LOCUS       DAOMVE010000001        38141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_126_length_38141_cov_3.467521, whole genome shotgun sequence.
ACCESSION   DAOMVE010000001 DAOMVE010000000
VERSION     DAOMVE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 38141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38141
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_38141_cov_3.467521"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLS64_00005"
     CDS             <1..282
                     /locus_tag="PLS64_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42246.1"
                     /translation="NKNNLNEKLKASDDLIYMTSDNKVDSIDVEPSYVLLLSYLKDTS
                     RDIWVLIYVKGDDVYVQADKRHANINLDTAGYDIYKSKYNKSQLEELIK"
     gene            536..1888
                     /locus_tag="PLS64_00010"
     CDS             536..1888
                     /locus_tag="PLS64_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL42247.1"
                     /translation="MLYRKIETVIEEHLKSDSKKILLIDGARQVGKTYIIRYVGKKIF
                     KNFIEINMVEDSLGNRLFENTKTVEDFYLQVSMLAGNKMGKKSDTLIFIDEIQAYPHL
                     LTLLKFLSQDGKFTFIASGSLLGVTLSQTTSIPMGSIRKVRMFPLDFEEFLYANGMNE
                     IIISAMQKKFENLESLDESMHNRMMDLFRKYLLVGGLPDAVNSYISERNIQSVREIQS
                     EIHEYYAADASKYDDENKLKIRRVYDLIPSNMENKKKRIVAKNIENKSGKRFSDYQDE
                     FEYLISAGIALNVQAISNPVFPLIESTGKNLLKLYLNDVGILTGILYGNNIRAVLDDE
                     TSVNLGSAYESVVASELAAHGYKLYYYDNRSKGEVDYLIDDYDSLSAVPIEVKSGKDY
                     TVHSALNTFVSNKDYHIKKAFVLSNERTVTSDGKINYIPIYYIMFFKNDVGKTGSFTF
                     "
     gene            complement(1952..2185)
                     /locus_tag="PLS64_00015"
     CDS             complement(1952..2185)
                     /locus_tag="PLS64_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRL42248.1"
                     /translation="MKDLLAVIAKGLVDDPDSVSVEADAPNEEGTVVYHIHVAENDMG
                     RIIGKQGRIAKAIRTIARSAAIRSEEKIMVEID"
     gene            complement(2209..2454)
                     /gene="rpsP"
                     /locus_tag="PLS64_00020"
     CDS             complement(2209..2454)
                     /gene="rpsP"
                     /locus_tag="PLS64_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRL42249.1"
                     /translation="MAVKIRLRRMGSKKAPFYRVVVADSRYPRNGRFIEEIGTYDILK
                     KPSEFNVDAEAVKKWIANGAQPTDTVKALLKKNGVIE"
     gene            complement(2540..3949)
                     /gene="ffh"
                     /locus_tag="PLS64_00025"
     CDS             complement(2540..3949)
                     /gene="ffh"
                     /locus_tag="PLS64_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRL42250.1"
                     /translation="MAFEGLADKLGNAFKKLKNKGKLSEKDVKEAMREVRLALLEADV
                     NYKVAKDFTNKVTERAVGQDVMQSLTPAQMVVKIVDEELTSLMGGEDARIEFASKPPT
                     VIMMCGLQGSGKTTHTAKLAKMLKKQGRNPLLAACDVYRPAAIDQLKVVGEKAGVPVF
                     EMGKANPVKIAKEAIKRAKDYGNDVVILDTAGRLHIDEALMDELKNIKKEVEPNEILL
                     VIDSMTGQDAVNVAKSFNELLDITGVILTKLDGDTRGGAALSVKAVTGRPIKFAGTGE
                     KLDDIEVFHPDRMASRILGMGDVLTLIEDAQNKMDAEKAEEMAQKMMSNKFDFNDLYD
                     QFEQVKKMGPLKGILSKIPGVGKQLEGVDIDDRQIDWVQAIILSMTPEERSHPHIMNP
                     SRKRRIAAGSGRSIEEVNRLIKQIDQMQKMMRQMTKGGKGSKKRRKMKGLPGLGGMPM
                     NGMGPAGGMGGPDGMPPFK"
     gene            complement(3960..4310)
                     /locus_tag="PLS64_00030"
     CDS             complement(3960..4310)
                     /locus_tag="PLS64_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HRL42251.1"
                     /translation="MEKNIEVSLLFDFYGELLKPSGKKAIDLYYNEDLSLAEVADETG
                     ITRQGVRDSIKRSEQQLFEFEEKLGLFKQFSKLEQGLDEISALALEIKELSQDTQITE
                     LADVIIEKSGSLKD"
     gene            4769..5743
                     /locus_tag="PLS64_00035"
     CDS             4769..5743
                     /locus_tag="PLS64_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HRL42252.1"
                     /translation="MENAVALFGSFDGNIKLIEKEFNVRVTCRDSELKVEGDAENTSK
                     ACKVIESLLQFINRGEALSEQNIRYCISLVNEGSEDKIEQLAGDCICITSKGKPIKPK
                     TIGQKRYCSQIAKNTVTIGVGPAGTGKTYLAVAMAVTAFRSKQINRIILTRPAVEAGE
                     KLGFLPGDLQSKVDPYLRPLYDALFDMLGAETYQKYVERGNIEVAPLAYMRGRTLDDS
                     FIILDEAQNTTREQMKMFLTRLGFNSKMVITGDITQIDLPNGAKSGLKDCIKILKNID
                     DIGTCFFDEKDVVRHRLVQDIIKAYAKFENTDINNLRNNGSKNNGSKN"
     gene            5805..6320
                     /gene="ybeY"
                     /locus_tag="PLS64_00040"
     CDS             5805..6320
                     /gene="ybeY"
                     /locus_tag="PLS64_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRL42253.1"
                     /translation="MAEHNKIRVIITNKQKAVKIPTGIRMLVRRCCNAVLRLEKFEGS
                     AEISVTFTDNEGIKALNKQYRDKDIETDVLSFPMGENGVYDTNPETGAQILGDVVISM
                     EKARDQAELFGHSLQREVGYLTAHSVLHLLGYDHMEKLERVRMREKEELVMEQLGLPA
                     SSSYIVDDDMA"
     gene            6343..6837
                     /locus_tag="PLS64_00045"
     CDS             6343..6837
                     /locus_tag="PLS64_00045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRL42254.1"
                     /translation="MKKQIKSFKSAFRGFCFAVKSESHMRFHLVATFYVFVFGCFYDF
                     SAAKWALLAVLCALVIALEMVNTCVEKLCDLFCDRYDFSVKIIKDIAAAAVLAVSFPA
                     VFTAVLFFLDFEVIGKIFLYFVNNPVMLVLLILSAVISAVFVCAGPVKVKNAILGIKR
                     KDKH"
     gene            6887..7804
                     /gene="era"
                     /locus_tag="PLS64_00050"
     CDS             6887..7804
                     /gene="era"
                     /locus_tag="PLS64_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRL42255.1"
                     /translation="MLNTDNKDKSAFIAIVGRPNVGKSSILNRLMGQKIAIVSSKPQT
                     TRNRITGVLTQGEYQLVFFDTPGMHKPKTSLGKYMVRSVNESVGGVDCCMLVVEAGKE
                     PGDTELNLIEKFKSMEMPAILAINKIDTLKEKEELMKQIARYSLLYDFDAVVPVSAQD
                     GNGMNELLDELKKQAGEGGHFFDDDTLTDQPERVIVAEIIREKILRLCDREIPHGTAV
                     VIEKMKTRDNGMLDIDATIFCEKETHKRIIIGKNGSMLKKISTFARQDIERFFDCRVF
                     LQTWVKVKEDWRNRAQILQNFGYDEKNFD"
     gene            7908..8081
                     /locus_tag="PLS64_00055"
     CDS             7908..8081
                     /locus_tag="PLS64_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42256.1"
                     /translation="MDNWSAWQIFFTTGSVLDYIRYKSIQDAKDTGAITVNREETDEI
                     PNRGTDYKGTEYR"
     gene            8032..8778
                     /gene="recO"
                     /locus_tag="PLS64_00060"
     CDS             8032..8778
                     /gene="recO"
                     /locus_tag="PLS64_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRL42257.1"
                     /translation="MKFRTEGLIIKEQNIGEQDKLVFALTKSNGVIRAFVKGAKNIKN
                     GKCAAASLLSYSRLTIYKGRESYIIGEAQPIRIFSKLRSDVTKMCLAQYFCELALTIC
                     PREQKADSFLSLVLNSLYLLSENKRSADLIKPCLEMRLASMAGYMPDLRMCRECGEYI
                     TPLMYFLPRIGALECADCRTDVSEPAIALNEGTLTALRHTVYADDDKLFSFALPKDDL
                     VVLNAASESYLKYRFEKDFKTLVFYKTISS"
     gene            8924..11287
                     /locus_tag="PLS64_00065"
     CDS             8924..11287
                     /locus_tag="PLS64_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRL42258.1"
                     /translation="MNRHYKTLELDKILERLAGMTSIEDAREMALNLEPVFELKKVEQ
                     LLQQTDDAVALSGRFGAPSFGGAGNCSGHLRRAQAGGCLTTGELLSVASTLRTIRTVK
                     EWHSRSGGGASSLDGYFSGLVSNKFLEDKITSAIISEEEISDKASPVLSDIRRKIRTA
                     SSKAREVLDKIIHSSTYIKYLQDTIVTQRDGRYVVPVRSECRGNVPGLVHDTSSSGAT
                     VFIEPMGVVQANNDIKLLQSKEEQEIERILFELSANAGDFADSIIHSYKNLAQLNLIF
                     AKADLAYSMKASKPIMNDRGMIELKQARHPLIDKNKVVPVDVMLGKEFDTLVITGPNT
                     GGKTVTLKTIGLLTLMAMCGLLVPCADNSELSVFRRVLVDIGDEQSIEQSLSTFSGHM
                     TNIVQIIKLANAGSLCLIDELGAGTDPVEGAALAIAILERLRDKHAKIASTTHYAELK
                     EFALRTPGVENGSCEFDVATLKPTYRLLIGVPGKSNAFAISKRLGIDDEIISRASELV
                     SNENRQFEDVVEKLEKRRQSLEKQLENANRLTAKANTEKQKAENEMQKAKQRAEREIE
                     KARQEAQRIISRTRAQADAVAEELEKARKAKDMSVQARTQLKKNIDKMEAHADPVKAR
                     NTPDEEYKLPRPLKVGDTVLIYDIDKNATVLAPPNKDGVVLVQAGIIKTRVDIKNLRL
                     LKSNNKPAKDRFSSTRNVPSRMDVRPETEVDVRGETAFDAINIVDKAIDNAVLSGVSK
                     MTIIHGKGTGVLKREINKYLKTNKAVKSQRLGVFGEGEDGVTIIELK"
     gene            11326..11841
                     /gene="rimM"
                     /locus_tag="PLS64_00070"
     CDS             11326..11841
                     /gene="rimM"
                     /locus_tag="PLS64_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060024.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRL42259.1"
                     /translation="MKKQFLDSGKIVGTHGIKGEVRIDPWCDSPEFLCAFKKLWLDEN
                     GTEFIEVKSRPHKNIALAKIKGVDTIEAAEKYRGKVVYINRDDINLAEGVHFVQDLIG
                     LEVKDAGNGRVYGKISDVLRTGANDVYEIKDADGKTYLAPVIDDVVKEINVNDGYVLI
                     VPMKGIFDDED"
     gene            11831..12523
                     /gene="trmD"
                     /locus_tag="PLS64_00075"
     CDS             11831..12523
                     /gene="trmD"
                     /locus_tag="PLS64_00075"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRL42260.1"
                     /translation="MRIDIITLFPEMFEPVLNGSIIGRAQKSGVLEIVCHQLRDYAFD
                     KHKRVDDTPYGGGMGMLMKAEPIALCFEDICKQIGKRPHFVYMSPQGKTLKQQRLREL
                     ADIENICILCGHYEGVDQRLLDEFIDEEISIGDYVLTGGEIPAMVFADALSRMVPGVL
                     SNNECFTEESHFNGLLEYPQYTKPSVWRGKEVPEVLMSGHHANIEKWRKEKSIEVTAL
                     KRPEMLEEYNKK"
     gene            12786..13049
                     /locus_tag="PLS64_00080"
     CDS             12786..13049
                     /locus_tag="PLS64_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRL42261.1"
                     /translation="MYVKEVLVQNKAGLHARPATFFIQKANEYKSTIWVEKEERRVNA
                     KSLLGVLSLGIIGGTTIKVIADGSDETEAVEGLVSLVESGFAE"
     gene            13145..14989
                     /gene="uvrC"
                     /locus_tag="PLS64_00085"
     CDS             13145..14989
                     /gene="uvrC"
                     /locus_tag="PLS64_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRL42262.1"
                     /translation="MNPKMSQLRKKAMALPLLPGVYIMHDKSGKIIYIGKAKALKNRV
                     SQYFGSQNNHAEKVRRMVDNVDDFEYIITDSEFEALILECSLIKQHTPKYNILLKDDK
                     GYSYIRISSGDWKKLSYTLQKKDDGAEYIGPYKSSYYVKNAVEEANKIFMLPTCNRQF
                     PRDCGKGRPCLNYHIKQCMAPCTGRVRLKDYNENLNRALEFLRGGSTNSIKKLTEEME
                     TAAENLEFERAASIRDIIKSVRKMSDKQKVVATKIDDGDVIAGFSDDGKTCYQVFRFE
                     GGRLFDRESFVFDSGESESETEEFLLRYYTLRDSVPKSIAIDREFDGMEEIAEWLSQK
                     HGSKVGITVPQRGDRAQLVAMCKSNAAETLAQKKGATVREYNVLEELRDLLGLKKLPE
                     YIESYDISNLAGTENVAGMIVYKNGKPLKSAYRKFKIKGFEGQDDYASMAEVLTRRFD
                     EYYKNPDENEGFGKLPDLILLDGGKGQVAAVKEVLQSKNIDVPLFGLVKDDKHKTRAV
                     TGEGGEIALSPGRSLFAFLAKMQDEVHRFAIGYHHSRRSKNTFKSSLTGIEGIGEVRA
                     RSLLKYFRTIENISNADLAELENAPKMTKDAALAVYKYYHPQDENKTE"
     gene            15132..15680
                     /gene="rsmD"
                     /locus_tag="PLS64_00090"
     CDS             15132..15680
                     /gene="rsmD"
                     /locus_tag="PLS64_00090"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HRL42263.1"
                     /translation="MRVITGSARGRRLETLEGEDVRPTTDRIKEAVFSIIQFETEGRN
                     FLDLFAGSGQMGIEALSRGAKSASFVDNAKKSLETVKRNLKATKLENSAKVFAMDFHS
                     FLSMNSQRFDIAFLDPPYRTGMLQDALELVPEAMNDTGVIIAENPLDEEILSNYGDFV
                     LDRQYRYGKIKITTFRHKDFQR"
     gene            15677..16162
                     /gene="coaD"
                     /locus_tag="PLS64_00095"
     CDS             15677..16162
                     /gene="coaD"
                     /locus_tag="PLS64_00095"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRL42264.1"
                     /translation="MNKTVICPGSFDPVTLGHLDVITRASKLFDRVIVGVLVNSSKKP
                     IFSIDERIELLKEVTEHLNNVEIVGFNGLLAQYCEEHGVDAIVKGLRAVTDFEYEFQM
                     ALTNKKLNPNLETLFLTAEADSMYLSSSMVREIASMGGDISNFVPACIHDRIVERLKN
                     K"
     gene            16220..16681
                     /locus_tag="PLS64_00100"
     CDS             16220..16681
                     /locus_tag="PLS64_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HRL42265.1"
                     /translation="MKVEDLILQLQDVISAAKSMPFSGGKVMVSSDEIYDILDQIQDA
                     MPAEVRQAKNIVADRKQILAEANRESENIIRSAEERKKAMLNQNELVREAQAKAKEIV
                     DDAKQKSAEIRKAANVYVDSIMKRTEEGVATQLEALRKTHANIVSSQKKQG"
     assembly_gap    16896..16905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16906..17085
                     /locus_tag="PLS64_00105"
     CDS             <16906..17085
                     /locus_tag="PLS64_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42266.1"
                     /translation="FGHNILLRKNMVSKLKAFEMICKYYLFFRNFDIDFFAPMVYNKT
                     KGVRRCHSVTFKTTE"
     gene            17120..17539
                     /gene="mraZ"
                     /locus_tag="PLS64_00110"
     CDS             17120..17539
                     /gene="mraZ"
                     /locus_tag="PLS64_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRL42267.1"
                     /translation="MFSGMTNHSIDAKGRIVLPAKFREQLGETYYLARGFGNKCIQVM
                     SSEQFNAMCEKILALPANLSMAVQYTFNATAVEVTPNAQGRVIIPQSLREFAEIEGDA
                     VVIGMTNRLEIWSKKNYEDYVASQNENLALALKELKL"
     gene            17550..18473
                     /gene="rsmH"
                     /locus_tag="PLS64_00115"
     CDS             17550..18473
                     /gene="rsmH"
                     /locus_tag="PLS64_00115"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRL42268.1"
                     /translation="MEFSHIPVLLEQAVDALAVKENGIYVDGTAGGGGHSSEILSRLT
                     TGKLISIDQDPDAILTLTERFKKNENSIIIKGNFADMVSLLHSRGVGRVDGVLLDIGV
                     SSHQLDTAERGFSFHEDAPLDMRMSQSGTTAAELVNTLPAGELRRILSVYGEEKFAGA
                     IVNGIVKARAEKPIETTLELAEIIKENVPQRVRRDGHPARKSFQALRIAVNDELGVLE
                     RGLDGAFELLGSGGRLAVITFHSLEDRIVKQKMASWCQGCTCPKDFPVCVCGNKPKAK
                     LVVRKPVCANETELRNNPRARSAKLRICEKI"
     gene            18746..19294
                     /locus_tag="PLS64_00120"
     CDS             18746..19294
                     /locus_tag="PLS64_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42269.1"
                     /translation="MAYENRNAAYDLSLFDDSSDYVPGSAAPERKEEVHKKQERKRRK
                     NNVVSLPKEELQKNRRRRHNKFKIALGTVSGAAATIIIGIIIVGQVRLTELNQEVITA
                     KETLANAQSVYTQTQMKLESKLSTSDIEKYAEDKLGMTKATNTQKEFVTLAGGDKAEV
                     SASQESDNLFTQFWSSVQNLWS"
     gene            19428..21698
                     /locus_tag="PLS64_00125"
     CDS             19428..21698
                     /locus_tag="PLS64_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRL42270.1"
                     /translation="MQSPGSKNNSKKRAEKGPAQRLRQRTAILILLILVLGFGAAVLR
                     LTYLTTVQSSELQESAVDLQLADTTVSAKRGTIYDANGNVLAESASVWQVVMSPVNFK
                     NDKQRQAAAKGLSEIFDLEYNDVLDDTKQQSHYVVVKRRIESDEREKVLELIDTLKKD
                     YSCSGVIQLLDDYKRYYPKNSLASSVIGFTGSDDQGLEGIEYEYDSYLSGTPGRIITA
                     QNARGTDMPFRYEQNVESEDGNNVYLTIDETIQSICEKYMQKGVEDNNVLNKGVCIAM
                     DVNTGAILAMVTTDGYDLNNPYELSAKDKKKIKSTPKKKQAEAESAALSNMWRNKAVA
                     DTYMPGSVFKMCVASAALEENLVNEKTSFTCTGSISVEGETIHCSNISGHGTQNFVEA
                     ISNSCNPAFIQIGQMLGAGKFRQYYQGFGFSDKTGIDLPGEAEDSFWKEGKMGGVDLA
                     VASFGQNFTITPIQMITACAAVSNGGYVVQPHVVSKITDSKGNVIKTVDKKVKRQVIS
                     DDTSKKMNEYLEYNTERQGAAAGYISGYKVAGKTGTTEKRGVTKFESSFSEDYISSFC
                     GYAPADDPQIAMLVFFDTPDGDAYYGSQVSSPVFINIMSEVLPYLDVKTSYTDEELGY
                     VDASAGDYTGVSVDEAKTAVEADGFTATVKGNGSTVISQIPTVSSGLQKGGSIVLYTD
                     SDSQSETVSVPSLIGLSPDEVNDVASAYGLNVSFSGATTSSGTSSSQNIEAGTSVSPG
                     TVITVSFADSSSTLNE"
     gene            21734..22759
                     /gene="mraY"
                     /locus_tag="PLS64_00130"
     CDS             21734..22759
                     /gene="mraY"
                     /locus_tag="PLS64_00130"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRL42271.1"
                     /translation="MIYGIWTFCAALLAFIISAVTGKFLIPYLRKLHFGQTILEEGPK
                     WHKGKQGTPTMGGIMFIVAIVVVTMISTVIYAFTGADLVNSFFADIPRECMFIFCGLG
                     LAVANGFIGFIDDYIKVVKKRNLGLTAPQKLILQFLVAAAFLAVLAIAGYGTTTTIPF
                     VGKVDLGFFYYIIAAVAIVGIVNSVNLTDGIDGLDGSVTFFACVAFMLIACVKSYLGI
                     TAMSAASAGACLGFLVWNFHPAKVFMGDTGSLFLGGLVCALAFAIDMPILLIPIGIIY
                     IAEELSVILQVSYFKITHGKRLFKMSPIHHHFEMCGWSEVKIVGVFSAVTAVFGAIAF
                     ALAYFGA"
     gene            22810..24066
                     /locus_tag="PLS64_00135"
     CDS             22810..24066
                     /locus_tag="PLS64_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRL42272.1"
                     /translation="MAPQKRMMLKADVNRIYDEEVYSEQKIREEKFFVRPTRQRKMKK
                     GKLFSVGMGLDLPFCIIILVLLIIGMIMMFSASYAYAYYNQGDSYYYLVRQLIFAVIG
                     VGIMFVLSFFNYNKLHKVAPVVLAISYLCLLVVLVLPGSEGVKRWIPLGIFNVQPSEI
                     AKFAIILFFSHWCSKYYNKMQFARYSVLPGIIVFGSTALLLFLEPHYSGIVIIGILSV
                     VMLYIGGMQTKYLGIGLGLIVGVVLILAVTNSLSYAMSRLDGWGQALEYTTDDMWWTT
                     YQTRNSLYAIGSGGLWGLGLGQSRMKYLYIPEPQNDFIFAVVVEELGLVGALMILLIF
                     ALLVWRGITLSLRAKDKFGKLLGIGLTSQIGIQVVLNILVITDWLPNTGISLPFFSYG
                     GSSLIMLLAQMGLVLSISRTANIERQ"
     gene            24129..25250
                     /gene="murG"
                     /locus_tag="PLS64_00140"
     CDS             24129..25250
                     /gene="murG"
                     /locus_tag="PLS64_00140"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRL42273.1"
                     /translation="MKVLLAGGGTAGHINPALAIAGYIKNKRNDAEFLFIGNRGGMEQ
                     RLVPQAGFEIKSITISGFKRSFSPKSMLENVKTVSRTFTSSREAKKIIAEFKPDICIG
                     TGGYVSGPVIRTAAKMGIPCIIHEQNAYPGITNKMLAKSVKKVMLAVPDAKKYFDKNV
                     DFVITGNPVRQEILTAKKEESRKELGLDNRPVVLSFGGSLGARKINEAVADLVARSGI
                     DGRYQHIHAYGSYGDWFPQLVEEKGTDIADCSNLDIRPYIDNMPTCMAAADLVICRAG
                     AITLSEIQAMGKPAILIPSPNVAENHQYHNAMALVNAGAADIIEEKDLTGAALMRKTD
                     KMLLNPEKLEKYSENSRKMAITDANERIYSVVKKVLGLV"
     gene            25313..26566
                     /gene="murA"
                     /locus_tag="PLS64_00145"
     CDS             25313..26566
                     /gene="murA"
                     /locus_tag="PLS64_00145"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRL42274.1"
                     /translation="MSKLLVTGRKKLDGEVEVQGAKNSALPIIAASILTKGENVIHNC
                     PSLSDVDASVNILRYLGCAVTRCDKTLLVKCDDINRFDVPVELMRKTRSSIVFLGAIL
                     ARTGRARISFPGGCEIGSRPIDLHLNSLREMGADIREKHGYLECCVPNGLCGTKITLS
                     FPSVGATEDIMIAACLAKGTTTIINAAREPEICDLADYLNSCGADISGAGEGVVVIEG
                     VSSLCGSVHTIIPDRIVAATLMSCAAVTGSKIILNGIISSHLAALIPIFKNAGCKIRI
                     SDGNLEINAPERLKSMKTIRTMPFPGFPTDAQAPLLATACVADGTTVFVENIFKNRYR
                     HIPELVRMGAAVKTEGKVAVAEGVEILYGASVEAPDLRGGAALVVAGLCAEGKTEIGG
                     VEYIERGYECIESTLTSIGADIKKI"
     gene            26642..28054
                     /locus_tag="PLS64_00150"
     CDS             26642..28054
                     /locus_tag="PLS64_00150"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRL42275.1"
                     /translation="MSDGKDRRPLTSKERKSLEKQRKNAAKQAEKYHKEKAKQDKRAK
                     KSPNPKKEQQKKAPKSKIEEAFLKQKEEQESQRQVENMSREEKFRRKGKAKIKNLKPK
                     DYEDGYYVDEFGEKQKQEKRAKEIRAQEREVIRRRKKSLTSTQIKHRRIALYSSIFAA
                     VLIIGVILSLTVLFKTEKIEVVGDKYYDEKQIIAFSGVEYQENIFMGAMYNTSDKIVK
                     NLSYIESASVSFNIPDTITITVVDATPSYVIPNGNGYLLVSSKGRILEEISENKDKLP
                     ELTCGDIKTTEVGQYVSFSDANVPDILEDVSQSLINNKVKNITGFDVTDTANIKLVYD
                     GRIDINIGLPDDIDYKIRTAMTIINEKLDPNNTGLVAGTLDVSACSTSKISHYKPAAT
                     QPTAATQATTQATTQAADSSADTGDGYTGNYTWDDGTYSGGDTGYYDNNSYNNYDNGT
                     YDNGDGGYDYGNADGAYTGE"
     gene            28211..29317
                     /gene="ftsZ"
                     /locus_tag="PLS64_00155"
     CDS             28211..29317
                     /gene="ftsZ"
                     /locus_tag="PLS64_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRL42276.1"
                     /translation="MAFELELENEYMPKIKVIGVGGGGGNAVNRMVATEVKNVEFIAI
                     NTDEHVLRLSKASQKIQIGEKLTRGKGAGSMPQIGQEAAEESRDEIAALLKDTDMVFV
                     TAGMGGGTGTGAAPVVAKIAKDMGILTVGVVTKPFAFEGKRRMTQAEQGIAELSACVD
                     SLIIVPNERLKYVSDTSITLQNAFAIADDVLRQGVQSISDLILLPGLVNLDFADVTSV
                     MKDAGYAHMGMGSATGKDKATVAADMAISSPLLETSIDGAKGLIINITASADVSLDDI
                     DAASTMIKDKVSDDANIIWGAVIDDKMEDAISVTVIATGFNADGQIAKKNLTDAVSSA
                     SAPVAAKEEPKSTTKDKTDDDDDTFYDIMSIFNK"
     gene            29484..30440
                     /locus_tag="PLS64_00160"
     CDS             29484..30440
                     /locus_tag="PLS64_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HRL42277.1"
                     /translation="MKKLLALLIALMTVLLCSCSSGGETIRFGAAGTGGIYNEAANSI
                     KSIADADGTLKIETKTTAGSAANVRLLSQNYLDAAIVQSDIAHDAFNGENNFEKSGSL
                     EGYSAVVGMFTEACHIVVRKDSDINSVDDLLGKTVSIGEEESGSELNAKQILSAYGLN
                     SKMVKEKNLNYADAAKQLQNGEIDAAFFTLGLNATVVEELSKQCDIKLIGIDDAAVKK
                     LKNTYSYVDCKIPKNTYHGQSDEVGTVAVKAVLIVNDRLSVEQVKKLTQLVFDNAQEL
                     QLTVSADVDITLEKAVEGVKIPFHKGAAEYYSERGITVKTAG"
     gene            30462..31649
                     /gene="gltS"
                     /locus_tag="PLS64_00165"
     CDS             30462..31649
                     /gene="gltS"
                     /locus_tag="PLS64_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HRL42278.1"
                     /translation="MNITLDMYQTIAVAVIVLMAGAFLKKRISFLQKFCIPAPVIGGL
                     LFAILTLVLYMTGVAVIDFDDTLKEVCMVFFFTSVGFQANLKVLKSGGKSLIVFLFLV
                     IMLIVMQNFSAIGLANLIGLDSLTGMTTGSIPMVGGHGTAGAFGPVLEDFGVQGATTV
                     CTAAATFGLIAGSLMGGPVGNRLIKKHNLIATIKTEDDSLLVEEEEKHERHFSMYAPA
                     VFQLIIAVGLGTVVSDLLSLTGMTFPIYIGAMIVAAIMRNIGEYTGKITIHMGEINDL
                     GGICLSLFLGIAMITLKLWQLADLALPLIILLAGQVVLMFIFTYFVVFNIMGRDYDAA
                     VLAAGTCGFGMGATPNAMANMQAICEKYAPSVKAYLIVPIVGSLFADFLNSLTITFFI
                     NCI"
     gene            31667..31855
                     /locus_tag="PLS64_00170"
     CDS             31667..31855
                     /locus_tag="PLS64_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42279.1"
                     /translation="MKDRKFTFDSENLAFPEIHTGKVKQNDVAEDNTDSTVVYDTVAV
                     PEVHLKKHKHKKQKKDNK"
     gene            complement(31902..32876)
                     /gene="bsh"
                     /locus_tag="PLS64_00175"
     CDS             complement(31902..32876)
                     /gene="bsh"
                     /locus_tag="PLS64_00175"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HRL42280.1"
                     /translation="MCTAATYKTKDFYFGRTLDYEFSYGDEITITPRNFPFKFRHINE
                     CNSHFAMIGMAHIADGYPLYYDAMNEKGLCMAGLNFVGNAVYFEPKNGKENIAQFEFI
                     PYILSHCDSVLQARKKLENLNITNTQFSDKLPCAQLHWIISDRNESLTVESTNDGLKV
                     YDNPVGVLTNNPPFDIQMFMLNNYMSLSPKQPENNFGKSAELTTYSRGMGAIGLPGDL
                     SSASRFAKVAFTKLNSVSGDSESESVSQFFHILGSVDQQRGCCEVSDGKYEITLYTSC
                     CNADKGIYYYTTYNNHQICAVDMHKIDLDIAELTAYTPITEGKVLFQN"
     regulatory      33036..33106
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            33169..34383
                     /locus_tag="PLS64_00180"
     CDS             33169..34383
                     /locus_tag="PLS64_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRL42281.1"
                     /translation="MKNKFVDFKVLATSLCCSVVMGLISFAFLKCLDYAADFRSFFPL
                     CYMFLPVAGIVTAFVYKRIGGKSSMGNNIIIESANDGEKVPKRLASLTFIFTCITHLF
                     GGSVGREGTAVQIGGSLTSNVADYLGFKNNDRSTIVLSGISSAFGSVFGTPFAGAFFG
                     MEVCCVGRLSAGAVIPCFACSYLANFVTQLLGFKHERYAISSIPDFDARFLFVFLIAA
                     VCLGLIGKLFALGIKYVKLAYSKIFKNYLLAAAVGAAIVSLLIFALGLNDFEGLSTWM
                     QHTAFKGDAKWYDMPAKYLLTVLTLGAGFQGGEVTPMFDMGASFGSWFGCVCGFDPTF
                     FAAIGFVCVFAAAINTPITAIVLGIEVFGASAAPYFVLAVLISFIASGNTTIYPSQKF
                     PVDKSLFHFQKK"
     gene            34492..34890
                     /locus_tag="PLS64_00185"
     CDS             34492..34890
                     /locus_tag="PLS64_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42282.1"
                     /translation="MKKFRAVCLAFVMMLSVAVMLCGCGDTKITDKVNFSDVDEITVV
                     MSDESTAVLGEVDFKTVKNILQSAETGGTSGEFDGGILIETRKDGIVTHNIRVGGADK
                     ICVDKDNSTVYKISDGDYKTLEKVMDNYKL"
     gene            complement(34994..35176)
                     /locus_tag="PLS64_00190"
     CDS             complement(34994..35176)
                     /locus_tag="PLS64_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42283.1"
                     /translation="MGIFDFERLFDLNHDGTLDPLEKGLEFTVIEDIMNDDEDNDDLF
                     SSDLDDEFDTDFGDDF"
     gene            complement(35261..35446)
                     /locus_tag="PLS64_00195"
     CDS             complement(35261..35446)
                     /locus_tag="PLS64_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HRL42284.1"
                     /translation="MAENTIPNDPMILLSFVNTKLRDFYPSLDKMCDDLGCSKEEIES
                     KLSAIDYKYNAELNKFV"
     gene            35585..37051
                     /locus_tag="PLS64_00200"
     CDS             35585..37051
                     /locus_tag="PLS64_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRL42285.1"
                     /translation="MDKKFDVVIVGTGAAGLYAALNFPESVNILLVSKRELQLSNSSL
                     AQGGVACVLDTVHDSYKLHITDTLIAGKYKNTLSAVEKLVTEGPSDVLKIKDLGVDFD
                     LNEDGTMCKTLEAGHSRNRIVHHKDSTGKAIVDGLIAKVTQLKNVTVCDNALVYSIDK
                     VLNGFYISILRNGEKLHYGCNYCLLATGGIGRVYKYTTNSAIATGDGIAFAYMLGARI
                     KNLSRIQFHPTAFAADHGRERFLISEAVRGEGAVLLNCNGERFASNYDSRGELAPRDV
                     VSNAVMLESIKTNSENFYLDITHKPADFVRNRFPMIYERCLEEGVDITKDRIPVFPCQ
                     HYLMGGIDVDLHSRTTVEGLYAAGECSHTGVHGANRLASNSLLEALVYSRTAALDITD
                     KINKFGRRTLGEEPVYRPVEGKTMPHGCRSKIREILQDAYFVLPKPEKYEESSKQIHE
                     IMSELFAEKYEINSDLVEARSAAIVASIIFDEIMEGTD"
     gene            37051..37890
                     /gene="nadC"
                     /locus_tag="PLS64_00205"
     CDS             37051..37890
                     /gene="nadC"
                     /locus_tag="PLS64_00205"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014257134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRL42286.1"
                     /translation="MTLNSIYVDNLIKTALLEDINYLDTTTDYLIDENQENTAIFLAK
                     SSGVLCGIEVALRVFEILQPNGFEAKVYKHDGDKLVKGEIIAEIHGKTRTILKGERTA
                     LNLIQHMSGIATATNKAVEIVSGTKASIADTRKTLPGMRPLQKYAVTVGGGRNHRYNL
                     SDAAMLKDNHIDAGGGIANAVSKLKSKLGHMTKVELEVRNLDELNQALEAGVDVIMLD
                     NMSPELMKQAVDITAGKALLEASGGITDETLREIAETGVDIISMGALTHSVKAFDISL
                     KIK"
BASE COUNT        11274 a         7523 c         8769 g        10565 t
ORIGIN      
        1 aacaaaaaca accttaacga aaagttgaag gcatctgacg atttgatata tatgacatct
       61 gataataagg tggattcgat agatgttgaa ccgagttatg ttcttctttt atcatacctg
      121 aaagatacca gtcgagatat ttgggttttg atatatgtca agggtgatga tgtctatgtt
      181 caggctgaca aaaggcacgc aaacataaac cttgacacgg caggctatga tatctataag
      241 agtaaatata ataagtcaca gcttgaagaa ctgataaaat aatcacagtg taaagttgcc
      301 tgaatttaaa agattttata aagtacggtg taaccataaa acataggttg caccgtgttt
      361 tttatatcaa atttctgttc ataatttgaa aagtgtagct tttgcccctc aattttcatt
      421 gaaaagtgta gtttttaacc cctaaattct attgaaaagt gtgtctttaa atggtataat
      481 cttattgaaa agtgtaaaaa actatactga aaatctattg aaaagtggga ttggtatgct
      541 ttacagaaag attgaaaccg taatcgaaga acatttgaag tcggattcaa agaaaattct
      601 tctcatagac ggtgcaagac aggtcggaaa aacatacatt atccgttatg tcggcaaaaa
      661 gatttttaaa aattttattg aaataaatat ggttgaagat tccctcggca accgactttt
      721 tgaaaacaca aaaacagtcg aagattttta cttgcaggtg agtatgctcg caggaaataa
      781 aatggggaag aaaagcgata ccttgatttt tattgacgaa attcaggcat atcctcatct
      841 gctgactttg ctcaaatttt tgtcacagga cggcaaattt acattcattg caagcggttc
      901 gcttttgggt gtaacgctgt cacagactac ctcaattcca atgggcagta ttcgcaaagt
      961 gcgtatgttt ccgcttgatt ttgaggaatt tctctacgca aacggaatga atgaaattat
     1021 tatttcggct atgcagaaaa aatttgaaaa tcttgaatct cttgatgaat ccatgcacaa
     1081 taggatgatg gatttattca gaaagtatct gcttgtcggc ggattgcccg atgcggttaa
     1141 cagctatatt tctgagagaa atattcagtc cgtcagagag atacaaagcg aaattcacga
     1201 atattatgct gcagacgctt caaaatacga tgatgaaaac aaacttaaaa tccgcagagt
     1261 ttatgacctc attccgtcaa atatggaaaa caaaaagaaa aggattgttg caaaaaatat
     1321 tgagaataaa agcggtaagc gtttcagcga ctatcaggat gaattcgagt atttgataag
     1381 tgcagggata gcccttaatg ttcaggcaat ttcaaatccc gtttttccgc ttattgaatc
     1441 aacgggcaaa aatctgctca agctttatct gaacgatgtc ggtattctga cgggaatttt
     1501 gtacggcaac aatatccgtg ccgtgcttga tgatgaaacg agtgtgaatc tcggttcggc
     1561 ttacgaaagc gttgtggcaa gcgaacttgc ggctcacggc tataaacttt actattatga
     1621 taatcgaagt aagggagagg ttgactatct tattgatgat tacgacagcc tttctgctgt
     1681 tccgattgag gtaaaatcgg gcaaggatta cacggttcac agcgcattga acacatttgt
     1741 aagcaataag gattatcaca ttaagaaagc ctttgttctt tcaaacgaga gaacagtaac
     1801 ttccgacggg aagattaact atatcccgat ttactatatt atgtttttca aaaatgatgt
     1861 gggcaaaaca ggaagtttta ccttttgata tttaataggg tgtaaaaaag cctcccgtgg
     1921 tgggaggctt ttgttcggtt atgaccgtta atcagtcgat ttcaaccatg attttttctt
     1981 cactgcggat agcggcactg cgggcaattg tgcggatagc cttggcaatt ctgccctgct
     2041 taccgatgat tctgcccatg tcgttttctg caacatgaat gtggtaaaca actgttccct
     2101 cttcgttggg agcgtctgct tcaactgata cggaatcggg atcgtcaaca agaccctttg
     2161 cgataactgc aagtaaatct ttcattgttt gtcctttccg aagcgcaatt attcaattac
     2221 gccgttcttc ttgagaagag cctttactgt gtctgttggc tgagcaccgt tagcaatcca
     2281 cttcttaact gcttcagcgt caacattgaa ctcagaaggc ttcttgagaa tatcatatgt
     2341 gccgatttct tcaatgaaac ggccgtttct cggatatctt gaatctgcta ctactactct
     2401 gtagaaagga gccttctttg aacccattct tctaagtctg atctttactg ccataatgtt
     2461 cacctcataa aaattgttaa tttatattca ccaaccgtaa ttggatgaaa aactttgttt
     2521 aatgtttgat taatgttaat tatttgaacg gaggcattcc gtccggtccg cccattccgc
     2581 ctgcaggtcc catgccgttc atcggcatac cgccgagtcc gggaagacct ttcatttttc
     2641 ttcttttctt agagcctttt ccgccctttg tcatctgacg catcatcttc tgcatctggt
     2701 caatctgctt gataagtcgg ttgacctctt caatgcttct gcccgaacct gccgcaattc
     2761 tgcgctttct tgacgggttc ataatgtgcg gatgacttct ttcttcgggt gtcattgaga
     2821 ggataattgc ctgaacccag tcaatctgtc tgtcgtcaat gtcaacaccc tcaagctgtt
     2881 ttccgacacc gggaattttt gagagaatgc ccttgagcgg tcccatcttc ttgacctgct
     2941 caaactgatc gtacaaatcg ttaaagtcga atttgttgct catcatcttc tgagccattt
     3001 cttcggcttt ttcggcatcc attttattct gtgcgtcctc aatgagagta agcacatcac
     3061 ccatgccgag aatacggctt gccattctgt cggggtggaa aacttcaata tcgtcaagct
     3121 tttcgcctgt acctgcaaat ttaataggtc tgcctgtaac agcctttacc gaaagcgccg
     3181 caccgccacg agtgtcaccg tcaagctttg tgagaataac acccgtaatg tcaagaagtt
     3241 cgttaaagct cttcgcaaca ttaactgcgt cctgacctgt catcgagtcg ataacaagca
     3301 aaatttcgtt tggttcaact tcttttttga tgtttttaag ctcatccata agagcctcat
     3361 caatatgaag tcggcctgcg gtatcgagaa taacaacatc gttgccgtag tcctttgcac
     3421 gcttgattgc ctcttttgca atcttaacgg gatttgcttt tcccatttca aaaacaggaa
     3481 cgcctgcctt ttcaccgaca accttcaact ggtcgatagc ggcgggacga tatacgtcac
     3541 aggcggcaag cagaggattt ctgccttgct ttttcagcat ttttgcaagc tttgccgtgt
     3601 gggttgtttt acccgaaccc tgaagaccgc acatcatgat gactgtcgga ggctttgacg
     3661 caaactcaat tcgtgcgtcc tcaccgccca taaggcttgt aagctcctcg tcaacaatct
     3721 taacaaccat ctgtgcagga gtaagacttt gcataacatc ctgaccgaca gcacgctctg
     3781 ttactttgtt tgtaaagtcc tttgcaactt tgtagtttac atcggcctca agaagtgcga
     3841 gtcgaacctc tcgcattgct tccttaacat ctttttcaga aagcttgcct ttgtttttca
     3901 gttttttgaa cgcattgcca agcttatctg caagtccttc aaatgccatg aaaactcctt
     3961 taatctttca gcgagcctga tttttcgatg ataacatccg caagctctgt gatttgtgta
     4021 tcctgtgaaa gttcttttat ttcaagtgca agagccgaaa tttcgtcaag cccctgctca
     4081 agcttactga actgtttgaa cagaccgagc ttttcttcaa actcaaaaag ctgttgctca
     4141 ctgcgcttga tactgtcacg cacaccctgt cttgttattc ctgtttcatc ggcaacctct
     4201 gcaagcgaca aatcttcatt ataataaagg tcaatcgcct ttttgcccga gggctttaaa
     4261 agctcgccgt aaaaatcaaa caagagagat acttcaatgt ttttttccat acctgacagc
     4321 tcccgtgatt ctggtctgcg tgtaaagaaa atttctttac acctgtgaat tataatatac
     4381 aattgcccgt ttgtcaagca ttttaagccg attttacggc tgagagtaat tttgataaat
     4441 ttttaatttt cgggaagtta tcaaatttgt acagttatat tacaaaaacg gcaatattgt
     4501 ggtagtttcg gaagatttaa ataactaaaa taagccgatt gtaatgcgat tttgagtata
     4561 attacgaaag agtaaaaaaa gtgtttctat cctttacaaa tgagtggcta tgtgaagttt
     4621 agttttcgtc aaaattcaca aaaattattt ttttagtgaa aaaagctatt tttaatgtac
     4681 attttcaagt aaaatgtgtt ataattgttt tgaattattg aagaaacggg agttttgctt
     4741 tacggaacaa attataaata tcgacagaat ggaaaatgcc gtagcacttt ttggcagttt
     4801 tgacggaaat attaagctga ttgaaaagga gtttaatgtc cgtgtcacct gccgtgacag
     4861 tgagcttaag gttgagggcg atgccgaaaa cacatcgaag gcctgcaagg ttattgaaag
     4921 cctgttgcag ttcatcaacc gtggtgaagc actttccgag caaaatatcc gctactgcat
     4981 ttcgcttgtc aatgaaggca gtgaggacaa aatagaacag cttgcaggcg actgcatctg
     5041 tattacttca aaaggaaagc ctattaagcc gaaaaccatc ggtcagaagc gctactgctc
     5101 gcagattgcg aaaaataccg tcacaatcgg cgtcggccct gccggtactg gcaagaccta
     5161 ccttgccgtt gcaatggcgg tcacggcttt cagaagcaag cagattaacc gaataattct
     5221 cacaagacct gctgttgagg caggtgaaaa gctcggtttt ctgcccggtg atttgcagag
     5281 caaggtcgat ccgtatcttc gccccctgta tgacgcattg tttgatatgc tcggtgcgga
     5341 aacatatcaa aagtatgttg aaagaggtaa cattgaggtt gcaccgcttg catatatgcg
     5401 aggaagaacg cttgacgaca gctttataat tcttgacgag gctcagaata cgacaaggga
     5461 acagatgaag atgttcctta cccgactcgg atttaactca aaaatggtaa tcacgggcga
     5521 tattacgcag attgaccttc cgaacggtgc aaagagcggt cttaaagact gcatcaaaat
     5581 cctcaagaac attgacgata tcggcacctg tttctttgac gaaaaggatg ttgtccgtca
     5641 tcgccttgtt caggatatca tcaaggctta tgcaaaattt gaaaacactg acattaataa
     5701 tttacgaaac aacggctcca aaaacaacgg ctccaaaaat taatttactt aattttcaca
     5761 tatcccacgc tatcgggtta aaaataatga aaggatgatc atgaatggca gagcataata
     5821 aaatcagggt aattatcaca aacaaacaaa aagcagtaaa gattccgaca ggtatcagaa
     5881 tgttggttcg tcgttgttgt aacgcagttc tgcgacttga aaaatttgaa ggttctgctg
     5941 aaatcagcgt tactttcacg gataacgagg gcatcaaggc tcttaacaag cagtatcgtg
     6001 acaaggacat tgaaaccgat gttctttctt tcccaatggg cgaaaacggt gtttatgaca
     6061 ccaatcccga aacaggcgca cagattctcg gtgatgttgt aatttctatg gaaaaggcaa
     6121 gagatcaggc agaactcttt ggtcacagcc tccagagaga ggttggctat cttaccgctc
     6181 acagtgttct gcaccttctc ggctatgatc acatggaaaa gcttgaaagg gtaagaatga
     6241 gagaaaaaga agagcttgtt atggaacagc tcggtcttcc tgcgtcatca agctacatag
     6301 ttgatgacga tatggcttga cagagtagtt atattacggg gtatgaaaaa gcaaatcaaa
     6361 agttttaaaa gtgcattcag aggtttctgc tttgccgtaa aaagcgaaag tcatatgaga
     6421 tttcaccttg ttgcgacatt ttatgttttc gttttcggtt gcttttacga tttttctgcc
     6481 gcaaaatggg cgttgctcgc cgttttgtgt gcgcttgtaa ttgcacttga aatggtcaac
     6541 acctgtgttg aaaagctttg tgatttgttt tgtgacagat acgatttttc cgttaagatt
     6601 atcaaggaca ttgcagccgc agccgtgctt gccgtgtcat ttccggctgt gtttacagcg
     6661 gtgctgttct tccttgattt tgaagtaatc ggaaaaatat ttttgtattt tgtaaacaat
     6721 cccgttatgc tcgttttgct gattttatct gcggtgattt ctgccgtctt tgtatgtgca
     6781 ggtcccgtaa aagtcaagaa cgcaattttg ggcattaaaa gaaaagataa acattaacaa
     6841 aggggcggca ttttgcttgc ccccgatttt tgtatagtga ggtaatatgt taaatacaga
     6901 taataaagat aaatccgcat ttattgccat tgtcggcaga cctaatgtgg gcaaaagttc
     6961 aattctcaac agacttatgg gacagaaaat tgcgattgtt tcaagcaaac cgcagactac
     7021 ccgcaacaga atcacaggcg tgcttactca gggcgaatat cagcttgttt tctttgatac
     7081 accgggtatg cacaagccga aaacctctct cggcaagtat atggtgcgtt ccgtcaatga
     7141 atccgtgggc ggtgttgact gctgtatgct cgttgttgag gcaggaaaag aaccgggtga
     7201 cacagaactt aaccttattg aaaagttcaa gtcaatggaa atgccggcaa tccttgcaat
     7261 caacaagatt gacactctca aagaaaaaga agaacttatg aaacagattg cccgttacag
     7321 tctgctctac gattttgacg cagttgttcc cgtgagtgca caggacggca acggtatgaa
     7381 cgaacttctt gatgagctta aaaagcaggc aggcgagggc ggtcatttct ttgatgacga
     7441 tactcttaca gaccagcccg aaagagttat tgttgccgaa attatccgtg aaaaaatact
     7501 ccgtctttgc gacagggaaa ttccgcacgg cacggctgtt gtaatcgaaa aaatgaaaac
     7561 aagggataac ggtatgcttg atattgacgc tactattttc tgtgaaaaag aaacccacaa
     7621 gcgtattatt atcggcaaaa acggctcaat gctcaagaaa atcagcacat ttgcccgtca
     7681 ggatatcgaa aggtttttcg attgcagggt attcctacag acatgggtaa aggtaaaaga
     7741 ggattggcga aaccgtgccc agattcttca gaatttcggc tatgacgaaa agaattttga
     7801 ctgataatcc gattaaaagc gattcgggta cacgatttga cacaatgtca gatttttcat
     7861 aatataataa acggcatccg tgaaacacta actgcggagg tgtttttatg gataactgga
     7921 gtgcgtggca aatattcttt accacgggaa gcgttcttga ttacattcgc tacaaaagta
     7981 ttcaggatgc caaagacacg ggtgcaatca ccgtgaacag ggaggaaacc gatgaaattc
     8041 cgaacagagg gactgattat aaaggaacag aatatcggtg agcaggacaa gctcgttttt
     8101 gctctcacaa agtcaaacgg tgtaatccgt gcatttgtaa agggtgcaaa aaacatcaaa
     8161 aacggcaaat gtgcggcggc atctctcctc tcatattccc gacttacaat ttataaaggt
     8221 cgggaaagtt acataatcgg cgaggcacag ccgattcgca ttttctcaaa gctccgcagt
     8281 gatgttacaa aaatgtgcct tgcacagtat ttttgcgaac tcgctctgac aatttgcccc
     8341 cgtgagcaaa aggcagactc atttctgagc cttgtgctta attcgctcta ccttttgtca
     8401 gagaacaaac gcagtgcaga cctcatcaag ccgtgtcttg aaatgaggct tgcaagtatg
     8461 gcgggctata tgcccgacct gagaatgtgc agagaatgcg gagaatacat tactccgctt
     8521 atgtactttc tgccacgcat aggcgctctt gaatgtgccg attgcagaac ggatgtgtcg
     8581 gaacctgcaa tcgctctcaa tgagggaacg ctcacggcgc ttaggcacac ggtttatgcc
     8641 gatgatgaca agctgttttc gtttgcattg ccgaaggatg accttgttgt gctgaatgcg
     8701 gcgagcgaaa gttatcttaa atacagattt gaaaaagact ttaaaacgct tgttttttac
     8761 aaaacaataa gcagttaatc agacaatgca gttttgtgtg aaaatgactt atttatttga
     8821 aacagaatga ctgaaaactg taggggcgct cattgggcgc ccgcgggcgg ataatatccg
     8881 cccctacgga cacaaaattg caattattga atttggtaga attatgaaca gacattacaa
     8941 aacacttgaa cttgataaaa tcctcgaaag gcttgcggga atgacttcga ttgaggacgc
     9001 ccgtgaaatg gcattgaatc tcgaacccgt gtttgaactg aaaaaggttg aacagctttt
     9061 acagcaaacc gatgacgcag ttgcgttaag cggaagattc ggtgctccgt cattcggcgg
     9121 tgccggaaat tgttcgggac atttacgcag agcacaggca ggcggttgcc ttaccacagg
     9181 cgaacttctg tcggttgcat caacgctcag aacaatccgc acggtcaagg agtggcactc
     9241 acgctcaggc ggaggagcaa gctctcttga cggctatttc agcggacttg tttccaacaa
     9301 gtttcttgag gacaaaatca cctcggctat tataagcgag gaggaaattt ccgataaggc
     9361 aagccctgtt ttgtcggata taagaagaaa aatccgcaca gcctcctcaa aggctcgtga
     9421 ggtactcgat aaaattattc acagctcaac atacataaag tatcttcagg atactatcgt
     9481 cacacagcgt gacggcagat atgttgttcc cgtaagaagt gaatgcagag gcaatgttcc
     9541 gggtcttgta cacgacacct cgtcaagcgg tgcaaccgtg tttattgagc ctatgggcgt
     9601 tgttcaggca aacaatgaca tcaagctttt gcagtcaaag gaagaacagg agattgaaag
     9661 aatcctcttt gagctttccg caaatgcagg cgattttgcc gacagcatta tccacagcta
     9721 caagaacctt gcacagctta atttgatttt tgcaaaggct gaccttgcgt attcaatgaa
     9781 agcgtcaaag ccgattatga acgacagggg aatgattgag cttaaacagg ctcgtcatcc
     9841 cctcatcgac aaaaacaagg ttgttcccgt tgatgttatg ctcggaaaag agtttgacac
     9901 gcttgtaatc acaggtccta acacgggcgg taaaaccgta actttaaaga caatcggtct
     9961 gctcacgctt atggcaatgt gcggacttct cgttccgtgt gccgacaaca gcgagctttc
    10021 cgtgttcaga agggtgcttg tggatatcgg tgacgaacag agcattgaac agtcgttgtc
    10081 aaccttttcg ggacatatga caaatatcgt tcagattata aagcttgcaa acgcaggctc
    10141 gctctgcctt attgacgagc tcggcgcagg aacagaccct gtcgagggtg cggctcttgc
    10201 aattgcaatt cttgaaagac tgcgtgacaa acacgcaaaa atcgcttcaa caacccacta
    10261 tgcggagtta aaggaatttg ctctgcgtac tcccggagtt gaaaacggca gttgcgaatt
    10321 tgatgttgca actctcaagc cgacttaccg acttctcatc ggtgttcccg gcaagagcaa
    10381 cgcgtttgca atttcaaaaa gactcggaat agatgatgag ataatcagcc gagcaagcga
    10441 acttgtttca aacgagaaca gacagtttga agatgttgtt gaaaagcttg aaaagcgccg
    10501 tcaaagcctt gaaaaacagc ttgagaatgc aaacagactt actgcaaagg ctaataccga
    10561 aaagcagaaa gcagaaaacg aaatgcagaa ggcaaagcag cgtgcggagc gtgagattga
    10621 aaaggcaagg caggaggctc agcgtattat ttcacgcaca agagcgcagg ccgacgcagt
    10681 tgccgaagaa cttgaaaagg cgagaaaagc caaggatatg agcgttcagg caagaacaca
    10741 gctaaagaaa aatattgaca agatggaggc tcatgccgat cctgtcaagg ctcgcaatac
    10801 tcccgatgag gagtacaagc tgccaagacc gctcaaagtc ggcgacacgg ttcttattta
    10861 cgatatcgac aaaaacgcaa ctgtacttgc accgccgaac aaggacggtg ttgtgcttgt
    10921 tcaggcgggc ataatcaaaa cgagggttga tatcaagaat cttcgtttgc tcaaatcaaa
    10981 caacaagccc gcaaaggaca gattttcaag cacaagaaat gtgccgagca gaatggatgt
    11041 ccgtcccgaa acagaggttg atgtccgtgg tgaaaccgcc ttcgacgcaa tcaatattgt
    11101 tgacaaggca attgacaatg ccgtactttc gggtgtgagc aaaatgacga ttattcacgg
    11161 caaaggcacg ggtgtgctta agcgtgaaat aaacaagtat cttaaaacaa acaaagccgt
    11221 caaaagtcag cgactcggtg ttttcggcga gggcgaagac ggtgttacaa tcattgaatt
    11281 aaagtaaaat ccgactgaat ttattttgtg aaatttgagg taattatgaa aaaacagttt
    11341 cttgattcgg gaaaaatcgt gggtacacac ggcataaaag gtgaggtcag aattgatccg
    11401 tggtgtgatt cccccgaatt tctctgtgcc tttaaaaagc tgtggcttga cgaaaacgga
    11461 acagagttca tcgaggtgaa atcccgtccg cacaaaaata tcgctcttgc aaaaatcaag
    11521 ggcgttgaca caatcgaggc agccgaaaaa tacagaggca aggttgtgta tatcaaccgt
    11581 gacgacatca atcttgcaga gggcgttcac tttgttcagg atcttatcgg tcttgaggtt
    11641 aaggatgccg gcaacggcag agtttacggc aaaatttccg atgttttgcg tacaggcgca
    11701 aacgatgttt acgaaatcaa agacgccgac ggcaagacat accttgctcc cgtaattgac
    11761 gatgttgtaa aagaaatcaa tgttaatgac ggctatgttc ttattgttcc catgaaagga
    11821 attttcgacg atgaggattg atattataac gcttttcccc gaaatgtttg agcctgtttt
    11881 aaacggaagc ataatcggca gagcacagaa aagcggtgtg cttgaaatcg tgtgtcacca
    11941 actgcgtgat tacgcttttg acaagcacaa aagagttgac gacactccct acggcggcgg
    12001 aatgggaatg cttatgaaag ccgaacccat tgccctctgc tttgaggata tctgcaaaca
    12061 aatcggcaaa agaccgcatt ttgtctatat gtcaccgcag ggcaaaacat tgaaacagca
    12121 aagacttcgt gagcttgccg atattgaaaa tatctgtatt ctctgcggtc attacgaggg
    12181 tgttgaccaa agacttcttg acgagtttat tgacgaagaa atttcaatcg gcgactatgt
    12241 tctcacgggc ggagaaattc ccgcaatggt ctttgctgat gcactttcaa gaatggttcc
    12301 gggtgtgctt tccaacaacg aatgttttac cgaggaaagc cattttaacg gacttcttga
    12361 gtatccgcag tacacaaaac cgtcagtctg gagaggcaaa gaagttcccg aagtgcttat
    12421 gagcggtcac catgcaaaca ttgaaaagtg gagaaaagaa aagagcatag aggtgactgc
    12481 cctcaagcgt cccgaaatgc ttgaagaata taacaaaaag taatttttgc agtatattat
    12541 cggtgtgttt acataaaata agacaagaaa aaacatatga aataccgtgt gtttacgggg
    12601 aaaatataat attttgtaaa aatgcacaat aaacagggta ataaattctc tcaaggtgca
    12661 tattaacacg aaattaattt attgtattga ccttgttcgg aaaaaagatt ataatacata
    12721 ttaaattgag ccgcaatttg caggcattgg tggttaaaaa tttaaaggca aagagggttt
    12781 taaaaatgta tgttaaggaa gtattagtac agaataaagc cggtttacac gctcgtccgg
    12841 caactttctt tattcagaag gctaatgagt ataagtcaac aatttgggta gagaaggaag
    12901 agcgtcgtgt aaatgcaaaa agtcttctcg gtgtgttgtc cctcggaatt atcggcggca
    12961 ctactattaa ggtaattgct gacggttctg acgaaactga agcagttgag ggtcttgttt
    13021 ctctcgttga gtcaggcttt gctgaataat cagctttaaa ttttacaaaa taccaagggg
    13081 cggtttttcc gcctcttttt tgtttctctc aaattaatct gaaatcgtaa aaggcggtga
    13141 ttttgtgaat ccgaaaatgt cacagcttcg caaaaaagca atggcacttc ctctgttgcc
    13201 gggtgtgtac atcatgcacg acaaaagcgg aaaaattata tatatcggca aggcaaaggc
    13261 tcttaaaaac agagtcagcc aatacttcgg ttcgcagaat aaccatgccg aaaaggttcg
    13321 cagaatggtt gacaatgttg atgattttga atatatcatc acggacagcg aatttgaggc
    13381 actcattctt gaatgcagtc ttatcaaaca acacaccccg aagtataata ttctgcttaa
    13441 agacgacaag ggctacagct atataagaat cagctcgggt gattggaaaa agctgtcata
    13501 tacattgcag aaaaaggatg acggagcgga atatatcggt ccgtacaaga gcagttacta
    13561 tgtaaaaaat gccgttgagg aggccaacaa aatctttatg ctcccaacct gcaaccggca
    13621 gtttccgaga gattgcggaa agggcagacc ttgccttaac taccacataa agcaatgcat
    13681 ggctccgtgt acaggcagag taaggcttaa agattacaac gaaaatctta accgtgcgct
    13741 tgaatttttg cgtggcggtt caacaaattc aatcaaaaag ctgaccgaag aaatggaaac
    13801 agccgcagaa aatcttgaat ttgaaagggc ggcgtcaatc agagatataa taaaatccgt
    13861 ccgtaaaatg agcgacaagc aaaaggttgt tgcaacaaaa attgatgacg gcgatgttat
    13921 tgcaggcttt tcggatgacg gcaaaacctg ttatcaggta tttcgatttg aaggcggaag
    13981 gttgtttgac cgtgaaagct ttgtgtttga cagcggtgaa agtgaatccg aaaccgaaga
    14041 atttttactg cgatattaca ctctgcgtga cagtgtgcca aagagcattg caatcgacag
    14101 ggaatttgac ggtatggaag aaattgccga atggctctcg caaaagcacg gcagtaaggt
    14161 cggcataacg gttccgcaga ggggtgacag ggcgcagctt gtggcaatgt gcaaatcgaa
    14221 tgccgcagaa acccttgcac agaaaaaggg cgcaaccgtt cgtgagtaca atgtgcttga
    14281 agaactgcgt gatttgctcg gacttaaaaa actgcccgaa tatatcgaat cctacgatat
    14341 ttcaaacctt gcaggcacgg aaaatgttgc gggtatgatt gtttataaaa acggcaaacc
    14401 gctcaaatcg gcttacagaa agtttaaaat caaaggcttt gaaggacagg atgactacgc
    14461 atccatggcg gaagttttga caagacgatt tgacgaatat tataaaaatc ccgatgagaa
    14521 cgagggattt gggaaattgc ccgaccttat cctgcttgac ggcggtaaag gtcaggttgc
    14581 ggctgtaaaa gaggttttgc agagtaagaa tattgatgtt ccgctgttcg gtcttgtaaa
    14641 ggacgataag cacaaaaccc gtgccgtaac gggcgagggc ggagaaattg cgctcagtcc
    14701 cggacggtcg ctgtttgcgt tcctcgcaaa aatgcaggac gaggttcacc gctttgcaat
    14761 cggctatcac cattcaagac gaagtaaaaa tacattcaaa agctcgctta caggcattga
    14821 gggtataggc gaagtcagag caaggtcgct tcttaagtat ttcagaacga ttgaaaacat
    14881 ctcaaacgca gaccttgcag agcttgaaaa cgctcctaaa atgacaaaag acgccgcact
    14941 tgcggtgtat aaatactatc atccgcagga tgaaaacaaa acagagtaaa atattgttgt
    15001 aaattatctt ttaatgtgtt atactaaaat ttgcagtttt aaacaattgt ttgaaattca
    15061 acttttcggt aatgccgtat gcctgcttgc ggtgttgttt tatgattttg cggaaaggct
    15121 gcgtgtgttt tatgagagtt attacaggct ccgcaagagg cagacgactc gaaacccttg
    15181 agggtgaaga tgtaagaccg acaaccgaca gaattaaaga ggctgttttt tcgattatac
    15241 agtttgaaac cgagggcaga aactttcttg acctttttgc aggctcggga caaatgggta
    15301 ttgaggcttt gagcagaggc gcaaagtcgg caagctttgt tgacaatgca aaaaaatcgc
    15361 ttgaaacggt aaaaagaaac ctcaaggcta ctaagcttga aaactcggca aaggtttttg
    15421 caatggattt tcacagcttt ctttcgatga attcacagcg ttttgacatt gcatttctcg
    15481 atccgccata cagaacggga atgctgcagg atgcccttga acttgttccc gaggctatga
    15541 acgatacggg tgtaatcatt gcggaaaatc ctcttgacga agaaattttg tcgaattacg
    15601 gtgattttgt acttgacaga cagtatcgct atggtaaaat aaaaataact acattcagac
    15661 ataaggattt tcaaagatga acaaaacagt gatttgtccg ggaagtttcg atcccgtaac
    15721 gctcggacac cttgatgtta tcacgagagc gtcaaagctt tttgaccgtg taatcgtggg
    15781 cgtgcttgtc aattcttcaa aaaagccgat tttttcgatt gatgagagaa ttgaactttt
    15841 gaaggaagtt accgagcatc tcaataatgt tgaaatagta ggcttcaacg gtttgcttgc
    15901 acagtattgt gaggaacacg gtgtggacgc aattgttaag ggactcagag cggtaactga
    15961 ctttgaatat gaatttcaga tggcgctcac caacaaaaag ctgaatccga accttgaaac
    16021 cctgttttta acggctgagg cggattcaat gtatctcagc tcgagcatgg ttcgtgaaat
    16081 tgcctcaatg ggcggtgaca tcagtaattt tgttcctgcg tgtatccatg acagaattgt
    16141 ggaaagactg aaaaataaat aagcgtttga ttttttaatt aaaggtggat taaaccaaac
    16201 atataaggag tggacagaaa tgaaagttga ggatttgatt ttacagcttc aggatgttat
    16261 cagtgccgct aagtcaatgc cgttttcggg tggtaaggta atggtgagca gtgatgagat
    16321 ttatgatatt ctcgatcaga ttcaggacgc tatgccggca gaggtaagac aggcaaagaa
    16381 tattgtcgct gacagaaagc agattcttgc cgaggctaac cgtgaatcag agaatataat
    16441 cagaagtgcc gaggaacgca aaaaggctat gcttaaccag aatgagctcg ttcgtgaggc
    16501 tcaggcaaag gctaaggaaa ttgttgacga tgcaaagcaa aaatcggcag aaatcagaaa
    16561 agctgcgaat gtgtatgttg acagcattat gaaacgcacg gaagagggcg ttgcaacaca
    16621 gcttgaggct ctcagaaaga ctcatgccaa catagtcagc tcacagaaaa agcagggctg
    16681 atttaaattt agcttataaa ggacagactc aatatgagcc tgttcttttt ttatgccgtt
    16741 tttatcgtat gtgttgtgtc acattatgac actgaaacga atgcttgcgg aatcggcggt
    16801 tgaaaaatat aaaaataatg tcggcttttg taacccgtgc ggtgcgtttt gtaattttta
    16861 agactgctgt ttttgctatg gaatgggtga aacccnnnnn nnnnntttgg gcataatatt
    16921 cttttacgaa aaaatatggt ttcaaagttg aaagcatttg aaatgatttg taaatattat
    16981 ttgttttttc gtaactttga tattgatttt tttgcaccta tggtttataa taagacaaaa
    17041 ggtgtcagaa ggtgtcattc ggtgactttt aaaaccacag agtgacggtt tctgaagaaa
    17101 agtgtatgga ggtgggcaga tgttttcggg aatgacaaat cattctattg atgcaaaagg
    17161 aagaattgta ctgcccgcaa agttccgtga acagctcggc gaaacatact atcttgcacg
    17221 aggcttcggc aacaagtgca ttcaggttat gtcaagcgaa cagttcaacg caatgtgcga
    17281 gaagattctt gcacttcccg caaacctctc catggctgtt cagtacacct ttaacgctac
    17341 tgcggttgag gttactccca acgcacaggg cagggtgatt attccgcagt ccctgcgtga
    17401 gtttgccgag attgaaggcg atgcggtagt tatcggtatg acaaacagac tcgaaatctg
    17461 gagcaagaaa aattatgagg actatgttgc ttctcagaat gaaaatcttg cacttgctct
    17521 caaggagtta aagctttaag gaggttgtta tggaattttc tcatattcct gtccttcttg
    17581 agcaggctgt tgacgctctt gctgttaagg aaaacggcat ttatgttgac ggcacagcgg
    17641 gcggaggcgg tcacagcagt gaaatcctca gtcgacttac tacgggcaag ctcatttcga
    17701 ttgatcagga tcccgatgca atactgacgc tgacagagcg ttttaagaaa aacgaaaatt
    17761 caataattat caagggtaat tttgccgata tggttagcct tcttcattca agaggagtgg
    17821 gcagagtcga tggtgttctg cttgacatcg gtgtttcatc tcatcagctt gatacagccg
    17881 aaaggggttt ttctttccat gaggacgcac ctctcgatat gagaatgagc caaagcggaa
    17941 caacagccgc agagcttgtc aacactctcc ccgcaggtga gttaagacga atcctcagcg
    18001 tttacggcga agaaaaattt gccggtgcaa ttgtaaacgg aatcgtaaag gcaagagccg
    18061 aaaagccgat tgaaactacc cttgagcttg cggaaattat aaaagaaaat gttccacagc
    18121 gtgtccgccg tgacggacat cctgcgagaa aaagctttca ggctttgaga attgcggtaa
    18181 acgatgaact cggagttctt gaaagaggtc ttgacggtgc atttgaactt ctcggaagcg
    18241 gcggaaggct tgcagttatt accttccatt cgcttgagga caggatcgta aaacagaaaa
    18301 tggcatcttg gtgtcagggc tgtacctgtc caaaggattt tcccgtgtgt gtttgcggaa
    18361 acaagccaaa ggctaagctt gttgtaagaa agccggtttg tgcaaatgaa acagaattga
    18421 ggaataaccc gagagcaaga agtgcaaagt tgagaatatg cgaaaaaatt taaaaaaatt
    18481 ctctgtgact atagacaaaa ctgcctgttt gtagtaaaat ataacaaagt tgtaaagatt
    18541 attacagaca caagcaattg tgctttcgtg tttgttaata acaaaatgta tggctttgaa
    18601 atatatgtcc gataaaatcg gattgattgt gtgttgtgtg tattttataa atttattaaa
    18661 ggtttgaaac ttttttgttt tatcgaaaat aaagcaaaaa tgtgtgtttt atgaaattga
    18721 tatagaaagg catcgggtgc gtttaatggc ttatgaaaac agaaatgcag cttatgattt
    18781 gagtcttttt gatgacagca gcgactatgt tccgggttcg gccgctcctg aaagaaaaga
    18841 agaagtacat aaaaagcagg aaagaaaaag aagaaaaaac aatgttgtaa gtcttccgaa
    18901 ggaggaactt cagaagaaca gacgccgcag acataacaag tttaaaatcg ctctcggaac
    18961 agtgagcggt gctgcagcga caatcataat cggcatcata attgtcggac aggtaagact
    19021 taccgagctt aatcaggaag ttattacggc taaagaaact cttgcaaatg cacagagcgt
    19081 ttacacacag acacagatga agcttgaatc aaagctttca acatcggata ttgaaaagta
    19141 tgccgaggat aaactcggta tgaccaaggc tacgaacaca cagaaagaat ttgttaccct
    19201 tgcaggcgga gataaagcag aggtttctgc aagtcaggaa agtgacaatc tctttacaca
    19261 gttctggagt tcggtacaga atctttggtc ataacattta gattaattta atagatatgt
    19321 tatgaaagaa tggagagtta agactgcgtc ttgactcttt tttcggtata tatgcggatt
    19381 gacacggagc gtaacggaca ctattttgcg gtgaaaggag ctttacattg caatcaccag
    19441 gatcaaaaaa caactcaaaa aagcgtgccg aaaagggacc tgcacaaaga ctgagacagc
    19501 gtaccgcaat attgatttta ctcatacttg tgcttggctt cggtgcggct gttttaagac
    19561 ttacatacct caccaccgtt cagagcagtg aacttcagga gtcggctgtt gaccttcagc
    19621 ttgccgacac aactgtttcc gcaaaacgag gaacaattta tgacgcaaac ggcaatgttc
    19681 ttgccgaaag cgcatcggtt tggcaggtgg taatgtcgcc tgtcaacttt aagaatgaca
    19741 aacagcgtca ggctgccgca aaaggtcttt cggaaatttt tgaccttgaa tacaacgatg
    19801 tgcttgacga tacaaaacag cagagccatt atgttgttgt caaaagaaga atcgagtcgg
    19861 atgagcgtga aaaggttctt gagcttattg acactctgaa aaaggattac agctgttcgg
    19921 gtgttattca gctgcttgat gattacaaga gatattaccc gaaaaacagc cttgcatctt
    19981 ccgtaatcgg ttttacgggc agtgacgatc agggacttga gggtatcgag tacgaatatg
    20041 acagctatct gtcgggtact cccggtcgaa ttatcacggc acagaatgca agaggtacgg
    20101 atatgccgtt ccgatacgaa cagaatgttg aatctgagga cggcaacaat gtgtatctca
    20161 caattgatga aacaattcag tcaatttgtg aaaaatatat gcaaaagggt gttgaggaca
    20221 acaatgttct taacaagggc gtctgcattg caatggatgt aaacacaggc gcaatccttg
    20281 caatggtaac aacagacggc tacgatctta acaatcctta tgagctttcg gcaaaggata
    20341 agaagaaaat caaatcaact ccaaaaaaga aacaggcgga ggcagaaagt gcggctctca
    20401 gcaatatgtg gagaaacaag gctgttgccg acacatatat gcccggttct gtatttaaga
    20461 tgtgcgttgc gtcagcggct cttgaagaaa accttgtaaa tgaaaaaaca tcatttacct
    20521 gtacgggttc aatttcggtt gagggcgaaa caattcattg ttcaaatata tcaggtcacg
    20581 gaacacagaa ctttgttgag gcaatttcaa actcatgtaa ccctgcattt attcagatcg
    20641 gtcagatgct cggtgcaggc aagttccgtc aatattatca gggattcggt ttcagcgata
    20701 aaacaggaat cgacctcccg ggtgaggcag aggacagctt ctggaaagaa ggcaaaatgg
    20761 gcggtgttga ccttgcggtt gcatcattcg gtcagaactt cacaatcact cctattcaaa
    20821 tgattacagc ctgtgcggct gtttcaaacg gcggttatgt tgttcagcct catgtggttt
    20881 caaaaatcac agattcaaag ggcaatgtaa ttaagactgt tgataaaaag gttaaaagac
    20941 aggttatttc agacgataca tccaagaaga tgaatgaata ccttgaatat aacacagaaa
    21001 gacagggtgc tgccgcaggt tatatttcag gctacaaggt agccggtaaa acaggtacaa
    21061 ctgaaaaaag gggtgtaaca aagtttgaaa gctcattttc tgaggactat atatcatcat
    21121 tctgcggtta tgctccggca gacgatccgc agattgcaat gcttgtattc tttgatactc
    21181 ccgacggtga cgcctactac ggttcacagg tttcatcacc tgtatttata aatattatga
    21241 gcgaggttct tccttatctt gatgttaaga catcatacac agatgaagaa ctcggttatg
    21301 ttgacgcatc tgcaggcgac tacacaggag tcagcgttga cgaggctaaa acagctgttg
    21361 aggctgacgg ctttacggca accgtaaagg gtaacggttc aactgtaatt tcacagattc
    21421 cgaccgtatc gtcaggactt cagaagggcg gcagtattgt cctctacaca gacagcgaca
    21481 gtcagtcgga aacagtaagt gttccgagtc ttatcggtct ttctcccgat gaggtaaacg
    21541 atgttgcaag tgcctacgga ttgaatgtca gcttctcggg tgcaacaaca tcctcgggaa
    21601 caagctcatc acagaacatt gaggcgggaa cttcggtcag ccccggtacg gtaataacgg
    21661 tatcttttgc ggattcctct tcaacactca acgaataaca aaaatattta ttagtctgaa
    21721 aggaaagata actatgattt acggaatatg gacattttgt gcggctttgc ttgcttttat
    21781 aatctcagcc gttacaggca agtttttgat tccatacctt cgcaagctcc attttggtca
    21841 aacaattctt gaggaaggtc ctaagtggca caagggtaag cagggaactc ccacaatggg
    21901 cggtataatg ttcatcgttg caattgtggt tgtcacaatg atcagcacag ttatatatgc
    21961 ttttacaggc gctgatttgg taaattcatt ctttgcagac attccaagag aatgcatgtt
    22021 cattttctgc ggactcggtc ttgcggttgc aaacggtttt atcggcttta ttgatgatta
    22081 catcaaggtt gtaaaaaagc gtaacctcgg tcttacagcg ccacaaaagc tgattctcca
    22141 gttccttgtt gccgccgctt ttcttgcagt tctggcaatt gcaggctacg gtacaacaac
    22201 aaccattcct tttgtcggca aggttgacct cggatttttc tattatatta ttgccgccgt
    22261 tgcaatcgtg ggtatcgtaa actcggtaaa ccttacagac ggtattgacg gtcttgacgg
    22321 ttcggtaaca ttctttgcgt gtgttgcatt tatgcttatc gcctgtgtaa agagctatct
    22381 cggtataaca gccatgagcg ccgcatctgc tggtgcctgt ctcggattcc tcgtatggaa
    22441 ctttcaccct gcaaaggtgt ttatgggcga cacgggttca cttttcctcg gcggtcttgt
    22501 atgtgctctt gcttttgcaa ttgatatgcc tattcttctt attccgatcg gaattatcta
    22561 tattgctgag gagctttccg ttattcttca ggtcagctac ttcaaaatca cacacggcaa
    22621 gcgtcttttc aagatgagcc ctatccatca ccactttgaa atgtgcggtt ggagcgaggt
    22681 taagattgtc ggtgtgttca gtgcagtaac cgcagttttc ggtgcaatcg cttttgcact
    22741 tgcatatttt ggtgcataaa taaacgcaga aatacttgtt taaaataaat tttaagggag
    22801 gaacggtctt tggctccaca aaaaagaatg atgcttaaag ctgatgtaaa cagaatatat
    22861 gatgaagagg tttactcgga gcagaagatc agggaagaaa agttttttgt ccgtcctact
    22921 cgtcagagaa agatgaaaaa aggaaagctg ttttccgttg gcatgggact tgaccttccg
    22981 ttttgcataa tcattcttgt acttctcatt atcggtatga ttatgatgtt ttctgcaagc
    23041 tatgcctatg cttattacaa tcagggcgac agctattatt accttgtaag acagctcatt
    23101 tttgccgtta tcggcgtagg cataatgttt gttctttcgt tcttcaacta taacaagctc
    23161 cacaaggttg caccggttgt tcttgcaatt tcatatttgt gcctgcttgt ggttcttgta
    23221 ctgccgggtt ccgaaggtgt aaaaagatgg attcctcttg gaattttcaa cgttcagcct
    23281 tctgagattg caaagtttgc aattatcctt ttcttctcac attggtgcag taaatactat
    23341 aataaaatgc agtttgcccg ttacagcgtt ttgccgggta taattgtttt cggttcaaca
    23401 gcacttttgc tttttcttga accgcactat tcgggcatcg taattatcgg cattctctcc
    23461 gttgtaatgc tttatatcgg cggaatgcag acaaagtatc tcggaatcgg tcttggactt
    23521 attgtcggag tagtgcttat acttgccgtc acaaacagcc tgtcctatgc tatgagccgt
    23581 cttgacggtt ggggtcaggc acttgaatat acaacagatg atatgtggtg gaccacatat
    23641 caaacacgaa attcactcta tgcaatcggt tcgggcggac tttggggact tggtctcggt
    23701 cagtcaagaa tgaagtacct ctatattcct gagccgcaga acgactttat ctttgccgtt
    23761 gttgttgagg agctcggtct tgtgggcgca cttatgattc tccttatttt tgcacttctt
    23821 gtgtggagag gcatcacact ttcacttcgt gcaaaggata agtttggcaa acttctcggt
    23881 atcggtctta cctcacagat cggcattcag gttgtgctga atattctcgt tatcacagac
    23941 tggctgccta acacgggtat cagccttccg tttttcagct acggcggcag ttcgcttatt
    24001 atgctgcttg cccagatggg acttgtgctt tcaatttcca gaacggcaaa tatcgaaaga
    24061 caataattta aaaatagttt tctcctgcat gagttttgca gaattttcaa aagaaaggta
    24121 agaaaattat gaaagtattg cttgcaggcg gcggcacggc aggacacata aatcctgcac
    24181 ttgcaatagc cggatatata aagaataaaa gaaatgatgc cgagtttttg tttatcggca
    24241 accgtggcgg aatggagcaa agacttgttc cgcaggccgg ttttgaaatt aagtcaatca
    24301 caatcagcgg atttaagagg agcttttctc ctaagagtat gcttgaaaat gttaagacag
    24361 tttcaagaac cttcacttct tcaagagagg caaagaaaat aattgccgag tttaagcctg
    24421 atatctgtat cggaacaggc ggatatgttt cgggtcccgt tatcagaaca gccgcaaaaa
    24481 tgggtattcc gtgcattatt cacgagcaga atgcatatcc gggcattaca aataaaatgc
    24541 ttgcaaagag cgtcaaaaag gtaatgcttg ccgttccgga cgcaaaaaag tattttgaca
    24601 aaaatgttga ttttgtcatt accggaaatc ctgtaagaca ggaaatcctc acggcaaaaa
    24661 aagaagagtc aagaaaagaa ctcgggcttg acaaccgtcc cgtagtgctt tcgttcggcg
    24721 gaagcctcgg agcaagaaaa ataaatgaag ctgttgcaga ccttgttgca agaagcggta
    24781 ttgacggcag atatcagcac attcacgcat acggcagtta cggcgactgg tttccgcagc
    24841 tcgttgagga aaagggtact gacattgccg attgttcaaa tcttgatata agaccgtata
    24901 ttgataatat gccgacttgt atggcggcgg cagaccttgt aatctgccgt gcaggtgcaa
    24961 ttactctcag cgaaattcag gctatgggca aaccggcaat ccttatcccg tcacctaatg
    25021 ttgccgaaaa tcaccagtac cacaatgcaa tggcacttgt gaatgcaggt gcggctgaca
    25081 ttattgagga aaaagacctc acgggtgcgg ctcttatgcg taaaactgac aaaatgcttc
    25141 tcaatcccga aaagcttgag aagtacagcg aaaattcacg caaaatggca attaccgacg
    25201 ccaacgaaag aatttattcg gtagtcaaaa aagttctcgg acttgtgtaa cggtaacatt
    25261 tataacgctt tctcatagaa tagaagggta ttctttttga gagggtgtta ttgtgtcaaa
    25321 actgcttgtt acaggacgca aaaagcttga cggagaggtt gaagttcagg gggcaaaaaa
    25381 cagcgctttg ccgattattg ccgcctcgat tcttacaaaa ggtgaaaatg taatccacaa
    25441 ttgtccttcg ctttccgatg ttgacgcatc tgtcaacatt cttcggtatc tcggctgtgc
    25501 cgtcacacgc tgtgataaaa cacttctcgt aaaatgtgac gatatcaacc gctttgatgt
    25561 tcccgttgaa ttgatgcgta aaacaaggag ttcaattgtt tttctcggtg caatccttgc
    25621 acgaacggga agggcgagaa tttcatttcc cggcggatgt gaaatcggtt caagaccgat
    25681 tgatttgcat ctcaattcac ttcgggaaat gggggcggat attcgtgaaa agcacggcta
    25741 tcttgaatgt tgcgttccaa acggactttg cggaacaaag ataacgcttt cgttcccgag
    25801 tgtgggtgca acggaagata ttatgattgc cgcctgtctt gcaaaaggca caacaacaat
    25861 catcaatgcc gcccgtgagc ctgaaatctg cgaccttgcg gattatctca attcctgcgg
    25921 tgccgatatt tccggagcag gcgagggtgt tgtcgtcatt gagggtgtaa gctctctctg
    25981 cggctctgtc cacacaatca ttccggacag aatcgttgcc gcaacgctga tgagctgtgc
    26041 cgccgtaacg ggttcaaaaa ttatacttaa cggaataatt tcgtcccacc ttgcggcact
    26101 tattccaatc ttcaaaaacg cagggtgcaa gataagaatt tcggacggaa atcttgagat
    26161 aaacgctccc gaaagactta aatcaatgaa aacaatcaga acgatgccgt ttccgggctt
    26221 tcccacggac gcacaggcgc ccttgcttgc aacagcgtgt gttgcagacg gaacaacggt
    26281 ttttgttgaa aatatattta aaaacagata cagacacatt cccgagcttg tcagaatggg
    26341 tgcagccgtt aagacagagg gcaaggtagc cgttgcagaa ggtgtggaaa ttctttacgg
    26401 tgcgtcggtt gaagctcccg atttgcgcgg aggtgcggca cttgttgttg caggcctttg
    26461 tgcagagggc aaaaccgaaa tcggtggcgt ggagtatatt gaaagaggct acgagtgtat
    26521 tgagtctact cttacatcaa tcggggcaga tatcaagaaa atttagggta atcagatttt
    26581 taactgtcgc agggcggatt tccgccctcg gcagataaca taaacgaagg gaggatttgc
    26641 tttgagcgac ggtaaagaca gaagaccgct tacatcaaaa gagcgaaaga gtcttgaaaa
    26701 acagcgtaaa aatgccgcaa agcaggcaga aaagtatcat aaggaaaaag caaagcagga
    26761 taagagagct aaaaaatccc ccaatccgaa aaaggaacag cagaaaaaag ctccaaaaag
    26821 taaaattgag gaggcttttc tcaagcaaaa agaagagcag gaatcacaaa gacaagtcga
    26881 aaatatgagc cgtgaagaaa aattcaggag aaaaggcaag gctaaaatca agaaccttaa
    26941 accaaaggat tatgaagacg gctattatgt tgacgaattc ggagaaaagc aaaaacagga
    27001 aaagcgtgca aaggaaatcc gtgctcagga gcgtgaagta attcgcagaa gaaaaaaatc
    27061 tctgacgagc actcagatta agcacaggag aatcgcactg tacagttcaa tttttgcggc
    27121 agtgcttata atcggtgtta tactaagcct tacagtcctt tttaaaaccg aaaaaattga
    27181 ggttgtgggg gacaagtatt atgacgaaaa acagataatt gcattcagcg gtgttgaata
    27241 tcaggagaac atcttcatgg gtgcaatgta caatacatcg gacaagattg tcaagaatct
    27301 ttcgtatatc gaaagcgcaa gcgtaagctt taacattccg gacacaataa caattactgt
    27361 tgttgacgca acaccgtcat atgttattcc aaacggtaac ggctatctgc ttgtaagctc
    27421 aaagggcaga atccttgagg aaatttccga aaataaggac aagcttcccg aactcacctg
    27481 cggcgacatc aagaccacag aggttggaca gtatgtgtca ttcagcgacg caaatgttcc
    27541 cgatattctc gaagatgttt cgcagagcct tatcaacaac aaggttaaga atatcacagg
    27601 ctttgatgtt accgacacgg caaacataaa gctcgtttat gacggacgaa ttgacattaa
    27661 catcggactt cccgatgata tcgactataa aatcagaact gcaatgacaa tcatcaacga
    27721 aaagcttgac ccgaacaaca cgggacttgt tgcaggaacg cttgatgttt cggcttgcag
    27781 tacatcaaaa atttctcact acaaacctgc cgcaacccag cccacagccg caacacaggc
    27841 aaccacacag gcaacaacgc aggcggctga ttcttcagcc gatacaggtg acggctacac
    27901 aggaaactac acatgggatg acggaacata ttcgggcggt gacacgggtt attacgacaa
    27961 taacagctat aataactatg ataacggtac ctatgacaac ggtgacggcg gatatgacta
    28021 cggcaatgcg gacggcgcct atacgggcga gtaatttttc agcagaattt tccgtataat
    28081 tttgcagaaa aacggctgaa aagcattgct attttgaaaa aagtatgata gtataagata
    28141 gtataattgt gtactatgaa gaataatgat ttctgactga aagttgtcaa tatataagga
    28201 ggacgaaaaa atggcttttg aactggaatt ggaaaacgag tatatgccta aaattaaagt
    28261 gatcggtgtc ggcggaggcg gcggaaacgc tgttaatcgc atggttgcga ccgaggttaa
    28321 gaatgttgaa tttatcgcaa taaatacaga tgaacatgtt ttaagacttt caaaggcttc
    28381 gcagaagatt cagatcggcg aaaagcttac aagaggtaag ggcgcaggct ctatgccaca
    28441 gatcggacag gaggcagccg aggagagcag agatgaaatc gcagcacttc tcaaggatac
    28501 ggatatggta tttgttactg ccggcatggg cggcggtaca ggtacaggcg ctgctcccgt
    28561 agttgccaaa atcgcaaagg atatgggcat tcttacagtc ggcgttgtta caaagccgtt
    28621 tgcttttgaa ggcaagagaa gaatgactca ggctgagcag ggtattgcag agctttctgc
    28681 ctgtgttgac tcgcttatca tcgttcctaa cgaaagactc aagtatgttt cggatacaag
    28741 catcactctc caaaacgcat ttgctattgc cgatgatgtt ctcagacagg gtgttcagag
    28801 tatttctgac cttatccttc tcccgggtct tgtaaacctc gactttgccg atgttacttc
    28861 cgtaatgaag gacgcaggct acgctcacat gggtatgggc agtgcaacag gcaaggacaa
    28921 ggctaccgtt gccgcagata tggctatttc aagtccgctt cttgaaactt caattgacgg
    28981 cgcaaagggt cttattatca acatcacagc atctgccgat gttagtcttg atgatatcga
    29041 tgccgcatca acaatgatta aggataaggt ttcagatgat gcaaacatca tttggggtgc
    29101 tgttattgac gacaaaatgg aagatgctat cagcgtgacg gttattgcaa ccggctttaa
    29161 cgcagacggt cagattgcta agaagaatct tacggacgca gtttcatcag cgtcagctcc
    29221 tgttgcggct aaggaagagc caaagtcaac aacaaaggac aagactgatg atgacgacga
    29281 taccttctat gatattatgt caattttcaa caagtaatac attctgatgt aatgaggcac
    29341 tccttttcgg gagtgccttt tcgtttgaca acggcaattc cgttatggta taatttgaac
    29401 ggaataaagg ggaattgtga gcttttgtgc ttttacaatt tctctttttt tgagcgaaat
    29461 ccaaaagcaa agaaggcgga aaaatgaaaa aactacttgc acttttgatt gccttgatga
    29521 ctgttctttt gtgcagctgt tcatcaggcg gtgaaacaat ccgattcggt gcggcaggta
    29581 ccggcggaat ttataacgaa gcggcaaatt cgataaagtc gattgccgat gctgacggca
    29641 ctctgaaaat tgaaacaaag acaacagccg gttctgcggc aaatgttcgc ttgctttcac
    29701 agaattatct tgacgccgca attgttcagt ccgacattgc acacgacgca tttaacggcg
    29761 aaaataattt tgaaaaatcc ggcagtcttg agggttacag cgctgttgta ggtatgttta
    29821 cagaggcttg tcatatcgtt gtaagaaaag attctgatat taacagtgtt gatgatttgc
    29881 tcggcaaaac cgtgagcata ggtgaagaag aatcgggttc ggagcttaac gcaaagcaga
    29941 ttctttcggc atacgggctt aactcaaaaa tggttaagga aaaaaatctt aattatgccg
    30001 atgccgcaaa acagttacag aacggcgaaa ttgatgcggc attctttaca ctcggactta
    30061 atgcaacagt tgttgaggaa ctttcaaagc aatgtgacat caagcttata ggaattgatg
    30121 atgcagctgt taaaaagctt aaaaacacct acagctatgt tgactgcaaa attccgaaaa
    30181 acacttacca cgggcagagt gatgaagtcg gcacagttgc ggtaaaggct gttctgattg
    30241 tgaacgacag actttcagtc gaacaggtga agaagcttac acagcttgtc tttgacaatg
    30301 cgcaggaact tcagctgact gtttcggcgg atgttgacat aacgcttgaa aaagccgttg
    30361 aaggtgttaa aattcctttt cacaagggtg cggcagaata ttattccgaa agaggaataa
    30421 cagtaaaaac agcaggataa ctgcgaaagg attggtgctg attgaatata actcttgata
    30481 tgtatcagac aatcgcagtt gctgtgattg ttctgatggc cggtgcattt ctgaaaaagc
    30541 gtatatcgtt tttgcagaaa ttttgtatac ccgcacctgt aatcggcggt ttgctttttg
    30601 caattcttac gctggttctc tatatgacag gtgttgcggt aattgatttt gatgatacac
    30661 taaaggaagt ctgcatggtg tttttcttta cctcagtcgg atttcaggcg aatctcaagg
    30721 tactgaaaag cggaggcaaa tcccttatcg tatttttgtt tcttgtaatt atgctcattg
    30781 taatgcagaa tttctctgca atcggacttg caaatttgat cggacttgac tcgctcacgg
    30841 gtatgacgac gggttcaatt cctatggtag gcggtcacgg tacggcgggc gctttcggtc
    30901 ctgtacttga ggatttcggc gttcagggtg cgacaactgt ttgtactgcc gccgcaacat
    30961 tcggtcttat cgcaggttca cttatgggcg gacctgtcgg aaacagactt attaaaaagc
    31021 acaaccttat tgctacaatc aaaacagagg atgacagcct tcttgttgaa gaagaagaaa
    31081 agcacgaacg acatttttca atgtatgctc cggcagtatt tcagctgatt atagcggtcg
    31141 gactcggtac ggttgtttca gatttgcttt cacttacggg catgacattc ccgatttata
    31201 taggtgcaat gattgttgcc gcaattatga gaaatatcgg tgaatacacg ggtaaaatca
    31261 ccattcatat gggcgagatt aacgacctcg gcggtatatg tctctcactt ttcctcggta
    31321 ttgctatgat tacactcaag ctttggcagc ttgccgacct tgcgcttccg cttattatac
    31381 ttcttgcagg tcaggttgtt cttatgttca ttttcacata ttttgttgtg ttcaacatta
    31441 tgggcagaga ttacgatgca gctgttcttg cggcaggtac ttgcggtttt ggtatgggcg
    31501 caacgccaaa tgcaatggca aatatgcagg caatctgcga aaaatacgca ccgtccgtta
    31561 aggcttatct tattgttccg attgtcggca gtttgtttgc cgacttcctg aacagtctga
    31621 cgataacatt ctttataaat tgcatttaga atcggaggca ggtattatga aggacaggaa
    31681 atttacattt gattccgaaa accttgcttt tcccgagatt cacacgggca aagtaaagca
    31741 gaatgatgtt gccgaggata acacagacag cacggttgtt tatgatactg tcgctgttcc
    31801 cgaggttcat ctcaaaaagc ataagcataa aaaacagaag aaagacaata aataaaacaa
    31861 cagccgtaca tttcaaaagt gtacggctga aatttttgtg tttaattttg aaataaaacc
    31921 ttaccttccg taatgggtgt gtatgcggta agttcggcta tgtcaaggtc gattttgtgc
    31981 atatcaaccg cacaaatttg gtgattgttg taggtggtgt agtagtagat tcccttgtcg
    32041 gcattgcaac aggaggtgta aagtgtgatt tcatactttc cgtctgaaac ctcacaacaa
    32101 cctctctgct ggtcaacaga accgagaatg tggaaaaact ggctcacgct ttcgctctcg
    32161 ctgtcacccg aaacggagtt cagctttgtg aacgcaacct ttgcaaaacg ggatgcggat
    32221 gaaaggtcgc ccggcagtcc gattgcaccc attccacggc tgtatgtggt aagctcggca
    32281 cttttgccga agttgttttc gggttgcttt ggtgagagtg acatatagtt gttgagcatg
    32341 aacatctgaa tgtcaaacgg aggattgttt gtcagcacac cgacagggtt gtcatagacc
    32401 ttgagtccgt catttgtcga ttcaactgta agcgattcgt ttctgtcgga aataatccaa
    32461 tgcaattgag cacaggggag cttgtcgctg aactgcgtat ttgttatgtt aaggttttca
    32521 agctttttcc ttgcctgcaa aacgctgtcg caatgtgaaa gtatatacgg aatgaactca
    32581 aattgtgcaa tattttcctt gccgtttttc ggttcaaaat atacggcatt gcccacaaaa
    32641 tttagccctg ccatacacaa gcccttttca ttcattgcat cgtaatatag cggataaccg
    32701 tctgcaatgt gagccatgcc aatcatcgca aagtggctgt tgcattcgtt tatatggcgg
    32761 aacttaaacg gaaaatttcg tggagtaatt gtaatttcgt caccgtatga aaattcataa
    32821 tcaagcgttc tgccaaagta aaagtctttt gttttatatg ttgccgctgt acacatatca
    32881 tcagcctccg attcttttaa taataaatat tcttccgctt ggtatagagt atcatactgc
    32941 ttaaacaaaa tgattgcaag gtgatttttg gaaaattaca aaaattgtcg aaatcacttg
    33001 aaaaattacc ctgctcggta tatactatat atgtcaatat gaatgggaat gaggttctcc
    33061 cacggctttt gtcgaaaccg cttttgctga tgacttctgt tttttgaaca ggggcattgg
    33121 ctttttttat gcgatgttct ttgtgcggaa attttgagga ggaaatttat gaagaacaaa
    33181 tttgttgatt ttaaggtgct tgcaacatct ctgtgctgtt ccgttgtgat gggacttata
    33241 tcgtttgcgt ttctgaaatg tcttgattat gcggcagatt tcaggagctt ctttccgctg
    33301 tgctatatgt ttttgcccgt agcaggtatt gtgaccgctt ttgtatataa gcgtatcggc
    33361 ggaaagtcgt caatgggcaa caacattatt attgagagtg caaatgacgg tgaaaaagtg
    33421 ccaaagcgac ttgcatcgct gacatttata tttacctgca taacccacct tttcggcggt
    33481 tctgtcggca gagagggaac tgccgttcag attggcggtt cgctgacaag taatgttgcc
    33541 gattatctcg gctttaaaaa taatgaccga agtacaatcg ttctcagcgg aatcagctct
    33601 gcattcggtt cggttttcgg cacaccgttt gcaggtgcat ttttcggaat ggaagtctgc
    33661 tgtgtcggaa gattgtcggc aggtgcggta attccgtgct ttgcgtgttc atatcttgca
    33721 aattttgtta cacagcttct cggctttaaa catgaaagat atgcaatctc gtcaatccca
    33781 gattttgacg caagatttct tttcgtgttt ttaattgcgg cagtttgcct cggcttaatc
    33841 ggcaagctgt ttgcactcgg aataaagtat gtaaaacttg catactcaaa aattttcaag
    33901 aactaccttc ttgccgcagc agtaggtgcg gcaatcgtca gccttttaat tttcgctttg
    33961 ggacttaacg attttgaggg cttaagcaca tggatgcagc acaccgcctt taagggcgat
    34021 gcaaaatggt acgatatgcc ggcaaaatat cttttgaccg tactcaccct cggtgcagga
    34081 tttcagggag gagaggttac tcctatgttt gatatgggtg cgtcattcgg cagttggttc
    34141 ggatgcgtct gcggatttga tcccacattt tttgcggcaa tcggatttgt atgtgttttt
    34201 gccgcagcaa tcaacacacc tattacagca atcgttctcg gtatcgaagt tttcggtgcg
    34261 tctgccgccc cgtattttgt gcttgcagtc ctcatcagtt ttatcgcatc cggcaataca
    34321 acaatttatc cgtcgcaaaa attcccggtt gataaaagcc tttttcattt tcaaaagaag
    34381 taatgcaata ggcttgagca aagtgtaagt cggtgtattt aggcgttgac tttaccaaaa
    34441 ttattattta tacttttctc ataatcaata gttagttgtg ggggagatta tatgaaaaaa
    34501 ttcagagcgg tttgtttggc ttttgtgatg atgttatccg ttgcggtaat gctttgcggt
    34561 tgcggtgata cgaaaattac agacaaggtt aatttttccg atgttgatga aataaccgtc
    34621 gtaatgtcgg acgaatcaac cgccgtgctt ggcgaggttg atttcaagac ggtcaagaat
    34681 attctgcaat cagccgaaac aggcggtaca agcggtgagt ttgacggcgg aatccttatt
    34741 gaaacacgca aagacggaat tgttacccac aatatccgtg tcggcggtgc tgacaaaatc
    34801 tgtgttgaca aggacaattc aaccgtgtat aaaatttccg atggtgatta taagaccttg
    34861 gaaaaggtta tggacaatta caagctgtaa cacttgaaca gaatcacgat aaataaaaat
    34921 ataagcgaga tgaattcaat cgaaaaattc atctcgcttt ttgcttgaag attaaattgt
    34981 ttgctgaata aaatcagaaa tcatcgccga aatccgtgtc aaattcatca tccaaatcag
    35041 acgaaaacaa gtcgtcatta tcttcgtcat cattcataat atcttcaatc acggtgaact
    35101 caagaccttt ttcaagcgga tcaagcgttc cgtcatggtt taaatcaaac aatcgttcaa
    35161 aatcgaaaat gcccataaaa tttcctcctt aacatatggg atatcctata tgttaatcat
    35221 atcaaatgaa ctgtacgata aaacggactt tcctttgatg tcagacaaat ttattgagtt
    35281 ctgcgttgta tttgtagtca attgcagaaa gcttgctttc aatttcttct ttactgcaac
    35341 cgagatcgtc acacatttta tcaaggctgg ggtagaaatc acgcagcttt gtattaacaa
    35401 agcttaaaag aatcatcgga tcgttcggaa ttgtattttc agccataata atcgcccttt
    35461 caaaattatt tactgtattt actaatctta caattagtag tataatatac aggtacaaat
    35521 ttgtaaacgg tattttgtgt gatgccgtgt gtaacagata aatttttttc aaaggaactt
    35581 ggaaatggat aagaaatttg atgttgtaat cgttggcaca ggtgcggccg gactttatgc
    35641 ggcacttaat tttcccgaaa gcgtgaatat tcttcttgtt tcaaagcgtg aacttcagct
    35701 ttcaaacagc tctcttgcgc agggcggtgt tgcgtgtgtg cttgatacgg ttcacgacag
    35761 ctacaagctg catattacgg atacattgat tgcgggaaag tataaaaata ctctttccgc
    35821 tgttgaaaag cttgttaccg aaggtccttc ggatgttctt aaaataaaag acctcggtgt
    35881 tgattttgac cttaacgagg acggcacaat gtgcaaaact cttgaggcag gtcacagcag
    35941 aaacagaatt gttcatcata aggattcaac aggcaaggca attgttgacg gacttatcgc
    36001 aaaggttact cagctaaaaa atgttaccgt gtgtgacaac gctcttgttt attcaattga
    36061 caaggtgctg aacggttttt atataagcat tttgagaaac ggtgaaaagc ttcactacgg
    36121 ctgtaactac tgtctgcttg caacaggcgg tatcggcaga gtttataagt atacaaccaa
    36181 ctccgcaatc gcaacgggtg acggcattgc gtttgcatat atgcttggtg caagaattaa
    36241 aaatctttcg agaatacagt ttcacccgac agcttttgcg gctgatcatg gcagagagcg
    36301 ttttcttata agtgaggctg ttcgtggtga gggtgcggtt ctgctcaact gcaacggtga
    36361 gagatttgcc tcaaattatg acagcagagg agagcttgct ccgagagatg ttgtttcaaa
    36421 tgcggttatg cttgaatcaa ttaagacaaa cagcgagaat ttttatcttg acataaccca
    36481 caagccggct gattttgtaa gaaatcgttt tccgatgatt tatgaaagat gccttgaaga
    36541 gggcgttgac attacaaagg acagaattcc tgtatttccg tgccagcatt accttatggg
    36601 cggtattgat gttgaccttc attcacgcac aaccgttgag ggcttgtatg ccgcaggaga
    36661 gtgctcgcac acaggtgttc acggtgcaaa ccgtcttgca agtaactcgc tccttgaggc
    36721 gcttgtttat tcaagaacag ccgctcttga tattacggat aaaatcaaca aattcggcag
    36781 aagaactctt ggtgaagaac ctgtttaccg ccctgttgag ggcaagacca tgccgcatgg
    36841 ctgtcgttca aaaatcagag agattttgca ggacgcatat tttgttcttc caaagcctga
    36901 aaaatatgaa gaaagcagta agcagattca cgagattatg agtgaactgt ttgcagaaaa
    36961 gtacgaaatc aattccgatc ttgttgaggc aagaagtgcg gcaattgttg caagtatcat
    37021 ttttgatgaa attatggagg gtacagactg atgacactta acagcattta tgttgacaat
    37081 cttatcaaaa ccgcattgct tgaggatatc aactatcttg acacaacaac cgattacctt
    37141 attgacgaaa atcaggaaaa cacggcaatt ttccttgcaa aaagttcagg tgttctttgc
    37201 ggtatcgagg ttgcgctccg tgtgtttgaa attcttcagc caaacggttt tgaggcaaag
    37261 gtttataagc atgacggcga caagcttgta aagggcgaaa tcatcgctga aattcacggc
    37321 aagacaagaa caattttaaa gggcgagaga acggctctca accttattca gcatatgagc
    37381 ggcattgcaa ccgcaacaaa caaggctgtt gaaattgtca gcggtacaaa ggcttctatt
    37441 gccgacacaa gaaagacact cccgggtatg cgtccgctcc agaagtatgc cgttacggtt
    37501 ggaggcggaa gaaatcaccg ttacaatctt tccgatgccg caatgctcaa ggataaccac
    37561 attgacgcag gcggcggaat tgccaacgct gtttcaaagc ttaaatcaaa actcggtcat
    37621 atgaccaagg ttgagcttga agtaagaaac cttgacgaac tcaatcaggc tcttgaggca
    37681 ggtgttgatg ttattatgct cgacaatatg agccccgagc ttatgaaaca ggctgttgat
    37741 atcacggcag gcaaggcact tcttgaggca agcggcggca tcacggacga aactcttcgt
    37801 gaaattgccg aaaccggtgt cgatatcatt tcaatgggtg cgctcactca ttcggttaag
    37861 gcttttgata tttcacttaa aataaaataa gcctgaatca aaaaggttat gaaaagattt
    37921 tgaactgaaa ataaatccta ttgaattttt acgcaaggtt ttgcagtaat tttcaatagg
    37981 atttttgctt tatatttatt tcagatttat tgttcttgct aagaaataaa atcacagaca
    38041 aacaagtgcc tgctgaatat ttgtgcgata atatgcaaaa atattaaata aaattgactt
    38101 ttaaaagaaa atgcgttaaa ctgtatttac tgaaaatatt t
//
LOCUS       DAOMVE010000002        33437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_173_length_33437_cov_3.269307, whole genome shotgun sequence.
ACCESSION   DAOMVE010000002 DAOMVE010000000
VERSION     DAOMVE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 33437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33437
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173_length_33437_cov_3.269307"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="PLS64_00210"
     CDS             <1..1630
                     /locus_tag="PLS64_00210"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRL42287.1"
                     /translation="WLIILSLFFPADMPAFPVFTPGSNCRWASVAAWLPLVGMTGVIA
                     YFQVCRKSWLKKLLAVLAVFACVPVLNSMFQLMNSSIYYARWFYMGVLMLVLATIKAF
                     ENRKTDWNRAIRWSAGITVGATLLIGLMPVSYTDEESGDIQNTVIGTQATFERYWLYV
                     LMALLSLLAFVLIIKKFRRNKKRFTVMLTVGILSVSLFTSYFIIATGYFSSSSTNTIK
                     EDIINRRDGITIADIDNVRSDFYECVDNTAMFWQIQSINCFQSSVSTSIMQFYDALGI
                     TRDVASRPDLDVYGLRPFLSCKYLFDYRGDGKSGSLNSFVDENGNTRMPGWKYLRTQN
                     RFDIYRNEYYIPMGYTFDKFIAEEEFDLVTNAHKSEALLYAMVLPRDLMKKYSDITGY
                     SDEKYKLLYGKHPEDYDSITEKFDYSNSDYKKVCNLRALNSCTSFEYTDNGFKAVYNN
                     KKGDDNLLFFSVPYSDGFTATVNGKAADVEKVDYGFMAVKIPKGEKCDIVFTYETPGF
                     STGVKISLCAFGAFLIYCAVIVTVKTVKKRKNKN"
     gene            1670..3031
                     /locus_tag="PLS64_00215"
     CDS             1670..3031
                     /locus_tag="PLS64_00215"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HRL42288.1"
                     /translation="MFGNKILDFKDELLKDLNTLLSFESVDGEKNDECDNALNFILKR
                     AEDFGLTGERTTDKSGHITLGDSGKLCGVLTHLDVVPAGNSWSVPPYALTEKDGRLYG
                     RGIADDKGAALVTLYCLKAIKDSGVKGVNTLRAIYGTGEETGMEDMENYFKKNPLPDM
                     AFTPDSDYGICFAEKGILQLEVSTLLNNATTLSQFHAGRAVNAVPDRAYVMLDSSDYD
                     EQTLMRLADASDGDFEFNYTIDGLMIISRGKAAHACEPDKGYNAAAALVDLISNVYTT
                     KETGSICSFIDYAINKETNGRSLGLKMSDAVSGSLTVNLSSVNIEGQTAKAVFDIRYP
                     VTVSVNRVLKQFKKVAKISDLKVTVLNHEEPLYADKDSKLVKLLSDAYESVTGEKAEL
                     YSTGGGTYARMLGGHGVAFGPAFKNDDVRMHNADESMDKENFFKHAQICLEAMYRMFT
                     DGE"
     gene            3074..3862
                     /locus_tag="PLS64_00220"
     CDS             3074..3862
                     /locus_tag="PLS64_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42289.1"
                     /translation="MNTEKTVRKQAKKVLEGNRSVIISEIMVAVLAFLTGLFAFSLAM
                     SVAGLYDVKNPNQTQQMLTMIFGLVFFAFVVVCLPLINGVYRSVCNVVRGRECSPLDV
                     FYYYKKPKLFFKSVILDVISVGLFFIISGLLNVFNYLSAVSDKIIDNSPSLTAVVAVL
                     LVFAFIVSTAVVVVCYIIFVHYTFLAYGFNEHLPLGIYLPRMMAFSIKNFISTIKLFI
                     GFIGWAALCFFVVPAFYAVPYFLTTSAMSAKWLFELEGSRDIIC"
     gene            3856..4935
                     /locus_tag="PLS64_00225"
     CDS             3856..4935
                     /locus_tag="PLS64_00225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL42290.1"
                     /translation="MLVSLCMIAYNESNALSGLFRDIALQDYPHDKIEVVFVDSMSTD
                     DTKEKMEKFRDTDYGFWNVSVVQCVKRNQATSWNAALMTAKGDVIIRVDAHARIPRNF
                     VSRNVYNIKQGENVVGGGRPNITSNVSSWKLTLLAAEDSLFGSSVASYRRPLAQKEYL
                     DSLFHAAYRREVIAKVGGFNENLGRTEDNEFHYRIRMAGYKMCCCPDIISYQHSRNDL
                     HGMIRQKYSNGRWIGLTLSECPGCLSYFHFAPFLFVMALLGCSVLAFLGLPLFLYILL
                     IIYGMFDIVNAVGCCTMKNVQPQFVFLPFIFPLLHVAYGIGTIVGLIQIPSWRKKIKN
                     SGAKEHIEKVKRKIRENTLPGHSYK"
     gene            4986..5861
                     /locus_tag="PLS64_00230"
     CDS             4986..5861
                     /locus_tag="PLS64_00230"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HRL42291.1"
                     /translation="MTEKVELTPELLRELQLKQLDMLVYFRDFCEKNNLTFYLIGGAL
                     IGALRNGGFVPWDDDVDVMLPREDYEKLYKLWHEQGADGRFRLLKTDSDMFTGNIFMT
                     VTDTNYTMVKTNQTEVDIPHGLVLDVFPLDVCPDSRFARKMQYVWTMLYSLFLAQVVP
                     EKHGGIVGAGSKALLSIFRGKKLREKIWRTAEKHMIKYRLDKNKCVTELCSGPHWMKI
                     EYPKHIYSGVDYVTFEGIKMPCMSGYDEYLRMVFGDYMQLPPVEDRVPHHDIAYLDLN
                     SPCEIYDSKRKNKQK"
     gene            5871..>6270
                     /locus_tag="PLS64_00235"
     CDS             5871..>6270
                     /locus_tag="PLS64_00235"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRL42292.1"
                     /translation="MELIRDNWTKNDYDDFVEELRSYADEKYRSFHSSLIPDNDKDFF
                     IGVRMPVMRKLGKEISKGNGRSFLEQTTDNSYEEKTMRGIVIGLLKTESYEEFLMYSD
                     DFVQRIDSWAVCDGFCTNLKQTKKYKSEFFP"
     assembly_gap    6271..6280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6281..6723
                     /locus_tag="PLS64_00240"
     CDS             <6281..6723
                     /locus_tag="PLS64_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42293.1"
                     /translation="NGACRIELKVLSCNKVALRLYEKLSFKETKKYKSEFFPHVCEYT
                     KSDNPWIIRAGLVLMLDYYLEDEYIDTVLKCCDNSKNPHYYVSMARAWLIATALAKCR
                     EQTMRYIKSNSLDNATFNKMIRKCVESFRIDDETKKYLKTLKRY"
     regulatory      6757..6878
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6986..7648
                     /locus_tag="PLS64_00245"
     CDS             6986..7648
                     /locus_tag="PLS64_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRL42294.1"
                     /translation="MTKSKAKTINSDNTAKTTKLAVTAMLAALSAVLMFFEFPLGFIA
                     PSFYEFDFSEVPVLVGTFSMGPIAGAVIEFVKILVKFLIKGTSTGGVGELANFLIGCS
                     FILPAGFIYKYKKTRVGAIVGMLTGTVAMAAIGVVLNTFVLVPLYSSFMPLTEIIKMG
                     QAIFPAIDGTFTFCLYCVGPFNIIKGLIISVVVFIIYKPLSRLINSLDALLTKKKKAT
                     VQ"
     regulatory      7723..7820
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            7908..8480
                     /locus_tag="PLS64_00250"
     CDS             7908..8480
                     /locus_tag="PLS64_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HRL42295.1"
                     /translation="MSQLKKIQSSLLEMKSIYALAAIAMLLALRIVLGFFANSTLPFF
                     GNNVKLSAAFLPIAVTGVMFGPIPAAIVGALGDILSFIIAPTGAYFPGFTINGFITGF
                     IYGMFLYKNNVTPLRVVLAWFTNMLCVETFLAAFWLYTLYGGGSTPYTVYLTARFISV
                     AVKCIPEIILVFALGKLAGKIRVPHAIKRT"
     gene            complement(8735..10162)
                     /gene="glgA"
                     /locus_tag="PLS64_00255"
     CDS             complement(8735..10162)
                     /gene="glgA"
                     /locus_tag="PLS64_00255"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HRL42296.1"
                     /translation="MRILYCASEANPFCGSGGLADVAGSLPHTLCRKGQDCRIVVPLY
                     GTIPQELKNSMNFITNFTVPVAWRHQYCGLFWARWNDCTYYFIDNEYYFKRGTLYGHY
                     DDAERFAFFSRAILEMLPYIDFHPDIIHCNDWQTALVPVYYYLFYSHRPWYYNIKSLF
                     TIHNIQYQGEYGWEVNTECVGIPASETKILEMNGKLNMMKGAIETATRVSTVSPSYAA
                     EIKDPWFSWGLHDELNLRHYKLCGIKNGIDTESYNPETDYQLYRNYSKDDMSGKGECK
                     RALQERLCLEQRWDVPLVGMVTRLVAHKGLDLVRMGLEELMDTENMQFVILGSGDKEY
                     EDYFNYMQAKYPGRLCFCHGFVPELARKIYSGADIFLMPSAQEPCGLAQMIALRYGTI
                     PVVREVGGLKDSVFDSADNYGNGFTFKNYECADMQLAVRRALWGIQDDQGWHQLMWRA
                     MMSDNSWDRSAQDYINLYNDTLKWA"
     gene            complement(10191..11303)
                     /gene="glgD"
                     /locus_tag="PLS64_00260"
     CDS             complement(10191..11303)
                     /gene="glgD"
                     /locus_tag="PLS64_00260"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HRL42297.1"
                     /translation="MRSNNVLGFIFAATLDDKIPELASSRTTASVPIGGKYRIIDFDL
                     SNMSNSGISNVGIVAKSNFQSLMDHVGSGSAYDLSKRRSNLSILPPYDGKAFSSFVET
                     IYNMHGYIEHCREEYVLISQGNVITNIDYTDVFDFHSKKKADITLIYKNHAIPQGYDR
                     PITLDVDNDGKVNQIFVKPGVVDKGVFNHAYGSILIKKDLLMSEIRNAISVNQLDVRR
                     LIQSSLGKYSIYAYENKGYCAAISSINDYFEFNMDLMKKEVRDELFNPQRPIYTKVRD
                     DAPSRYGLTSNVKNSIIAQGCVINGEVENCVISKGVYVGEGAKLSNCIVMQDTKIGCN
                     TNLNYVIIDKDVTIKDGRSLMGYDSYPIYIAKKSVV"
     gene            complement(11293..12504)
                     /locus_tag="PLS64_00265"
     CDS             complement(11293..12504)
                     /locus_tag="PLS64_00265"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRL42298.1"
                     /translation="MFPKKEVVAMLLAGGQGSRLGVLTKKLAKPAVPFGGKYRIIDFP
                     LSNCVNSGIDAVGILTQYQPLVLNEYIGNGQPWDLDSMHSGVNCLSPYQAVDGADWYS
                     GTANAIYQNINYIERYNPEYVVILSGDHIYKMDYNKMLEYHKEKNAACTIAVIDVSLE
                     EASRFGILNTNEDGSIYEFEEKPAHPKSTNASMGIYIFSWKELKKYLTEDEFKEGSSH
                     DFGKDVLPAMLDAGERMFAYPFEGYWKDVGTIDSLWEANMDLLDPKVTLDLKDIYSRN
                     PMMPPHYVSNEASIQNSLVADGCNVYGNLEFSILFAGVTIGKNATINSSIIMPGAVIE
                     DGAEVQFAIIAENTVVGKNAKVGQNPEETENIDDWGITVVGANTVIKENTVIKAKTMI
                     DSDTEVDGNEK"
     gene            complement(12577..14499)
                     /gene="glgB"
                     /locus_tag="PLS64_00270"
     CDS             complement(12577..14499)
                     /gene="glgB"
                     /locus_tag="PLS64_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRL42299.1"
                     /translation="MNYSFNEARPALEVFHTGDSVRAYDFLGAHLVNRNDKNGVVFRV
                     WAPTARSVSVAGDFNNWNNEANYMYNIGYGVWEVFVEGVKEFCTYKYCIESEYGDRLM
                     KADPYAFHAQTRPGQASVVYDIESYSWNDSEWFNKRKENNISSSPMNIYEIHAGSWRK
                     YPDGNFFNYQKLADELIPYLKEMHYTHVQLMPIMEYPYDGSWGFQTTGYYAPTSRYGT
                     PSDFMAFVDKLHGEGIGVILDWVPSNFPTDDFGLARFDGSPLYESNDPKTSKRDSWGT
                     CLFNYARFEVTSFLVSCAMFWLDKYHIDGLRIGALSSMLYLDYGKTEGEWEPNKFGGK
                     ENLDAVDFVKRLNTAVHMYHPDVMMFAEENTSWPKLTHKIEDGGLGFDFKWNMGWMND
                     MLHYMSLNSMWRPFNHDSLTFSFYYAFSEKFLLPISHDEVSHGKGSLIKQMPGKYDEQ
                     FAGVRAFITYMYAHPGKKLVFMGTEIGQFDEWNHEEAIQWDLLEFEKHKKLRTFFKEL
                     NKFYLDCKPLYELDTVWKGFDWIHHDDYTNSVIAFKRTDKNGDEIVSVCNFQPIRRDE
                     YCIGVPKYGLYDEVFNSDEERFGGSGVVNGNNIKTEVMKIHGFDQGLSLTLPPLSVIY
                     LRCAKELEPDEAQKEN"
     gene            complement(14762..15793)
                     /gene="gap"
                     /locus_tag="PLS64_00275"
     CDS             complement(14762..15793)
                     /gene="gap"
                     /locus_tag="PLS64_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRL42300.1"
                     /translation="MSVKVAINGFGRIGRLAFRQMFGAEGYEVVAINDLTDPAMLANL
                     LKYDTAQGGYCGKIGENLHTVEAGEGSIIVDGKEITIYAKPNAAELPWGELGVDVVLE
                     CTGFYTSKAKSQAHIDAGAKKVVISAPAGNDLKTIVYSVNEKTLTADDTIISAASCTT
                     NCLAPMADTLNKYAEIQSGIMSTIHAYTGDQMILDGPHRKGDKRRARAGAANIVPNST
                     GAAKAIGLVIPELNGKLIGSAQRVPVPTGSTTILTAVVKGSDVTVEGINAAMKAAASE
                     SFGYNEDPIVSSDVIGMRFGSLFDATQTMVSKIADDLYEVQVVSWYDNENSYTSQMVR
                     TIKYFAELG"
     gene            16358..17905
                     /gene="rny"
                     /locus_tag="PLS64_00280"
     CDS             16358..17905
                     /gene="rny"
                     /locus_tag="PLS64_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRL42301.1"
                     /translation="MWISIICIVAAVVIGAGAGIFLGIAIRKNTAEKEIGSAEEEAKK
                     IVADAIKTAEARKKEVVLEGKDEVHRFRNESEKEISDRRKEVQRQERRIQQKEETLDK
                     KLDNMERKEEKVNKKLRDADAKLEEVETLKKSQFEMLEKISGYTAEQAKSYLLSQLEN
                     ELAHEKSVKIMEYTEQLKEEADDKARNIISLAIQRLAAEQVSEATISVVPLPNDEMKG
                     RIIGREGRNIRAIETLTGVDLIIDDTPEAITLSCFEPVRREIARIALEKLIADGRIHP
                     ARIEEMVEKARREVESEIKRTGERAVLDAGVHNIHPELVKLLGRLRYRTSYGQNVLDH
                     SLEVSYIAGMMASELGLDPTIAKRAGLLHDIGKSIDHEVEGTHIQIGVDLAKKYKESD
                     AVIHAIHAHHGDVEARTIVACLVQAADAISAARPGARRENVENYIKRLQKLEEVASSF
                     DGVERTFAIQAGREVRVMVKPEIVNDERMIPLARDICKKIEEELEYPGQIKVNIIRES
                     RASDFAK"
     gene            18537..19787
                     /locus_tag="PLS64_00285"
     CDS             18537..19787
                     /locus_tag="PLS64_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRL42302.1"
                     /translation="MDSGNIGFVLLCAAFVFVMTPGLAMFYGGLVRKKNVVNTMMTSI
                     FIIGVGIVMWVLFGYSLSFAPSGNAFIGDFRWLGLEGVSLTEGYTDDASIPNMVFSAF
                     QMMFAVITPALITGAVAGRMKFKSIFVFTIFWSLIVYYPLAHMVWGQGGLLGADGIGA
                     LDFAGGDVVHISSGVSALVLCILLGKRHDYEHSIYRIHNIPTVVLGASLLMFGWFGFN
                     AGSALAADGLAAHAFMTTGVSAAAAMLSWMLCDVIKNKKPTLIGASTGMVAGLVGITP
                     GAGFVPMWAAVIIGLTTSPVCYIMISYGKKKFGFDDALDAFSCHGTGGIWGGLLTGVF
                     SCTAINSSAGNGLVYGEFAQFGAQAAGIGITIVIAVVGTLICYGITRLLTGKIRVDLR
                     DELMGLDVSQHGESAYPSFNGLDN"
     gene            19803..20189
                     /locus_tag="PLS64_00290"
     CDS             19803..20189
                     /locus_tag="PLS64_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HRL42303.1"
                     /translation="MENNNDIQLIKIEAVVREEMFVDVKKALTDIGVNGITAYQVVGC
                     GIQRGMSEYVRGQKVDVQVLPKIKFEIVVSSEEWERKTIEAIRKTAFTGNPGDGKIFT
                     YYLKQAVKIRTGETGYDAIQTPNFDD"
     gene            complement(20262..20705)
                     /locus_tag="PLS64_00295"
     CDS             complement(20262..20705)
                     /locus_tag="PLS64_00295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRL42304.1"
                     /translation="MTEKLYKAKVSYYETDCMNIVHHSNYIRYFEDARIQYMHDIGCD
                     VAEMQSKGLYIPNVDAYARYKKPLKFGDEFSVEVSLVFFTGSRMIFDYKIFNNKNGEL
                     SATGRTTHCFATPELKPISIKHSNPDYYNRLRDNTVSPEDITIRR"
     gene            complement(20741..22402)
                     /locus_tag="PLS64_00300"
     CDS             complement(20741..22402)
                     /locus_tag="PLS64_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL42305.1"
                     /translation="MNLTYDSQMFRETFESEFTWLNGFMRNVRRFSDKPAAIDPQSGR
                     TWSYKELNADANRFANAMKKDGVKKNDIVFMQLYNSPQFLFGYIAPQKLGAISNPANF
                     NLSPGETAEIMGHNKPVVYVYDADIMQTAVQALEICEHKPKLILCVNNSKKEVTLPEG
                     HIFFDDYIKDSSYENPPVDYAPHIYDEVLRLQTSGTTGTPKGVPLNAINEVLSAHDVI
                     MHFPLSPVDITMNMTPWFHRGGIHSGGPTPTLYAGACLVIMRTFNPRSCMDFIEKYGI
                     TFITGVPSTLEMLANRQEKHQKNLSSLKGIITMGSPLEKAACIRYQELLSPNIFNGYG
                     TTESFWNTFLRPYDLPEMAGSAGRSCTDDEVRLVNVYDDRKAEPDDTVPCDNKTEGEI
                     IIKCPNKTTYCYVNNEKATKDKFYKGWMYTGDIGTWNSEQFVTVAGRKDDMIISKGEN
                     IYPARIEEILNSHPKVQECIVTGVPDSTRGECVAAYVIKADESLTVAELINFCDSSPN
                     ISKYQAPRYYRFVTELPHTATGKKQHFKIRQQAAEDLKNGLLLKK"
     gene            complement(22491..23717)
                     /gene="fabF"
                     /locus_tag="PLS64_00305"
     CDS             complement(22491..23717)
                     /gene="fabF"
                     /locus_tag="PLS64_00305"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRL42306.1"
                     /translation="MKKIVVTGMGAVTPVGIGVENYWNNITAGASGIDTIKTFDPSEL
                     AVQIAGEVKDFNPSDYLPKDLIRKTDPFMQYAYIAAEEAMKQSGIKIEPERTGIVMGT
                     AMAGIATIAYTQEALTGASHKKVGPRFIPKILGNIAAANIAIDYNIQGPSFTVSTACS
                     SGGDAINTACMCMQSGKADIMLAVGAESVLCPLVIYSLANAKALSRRNDSPSTASRPF
                     DVTRDGFVIGEGGGALVLETEEHALARGAKIYAEIRGCGNTDDAYHVTAPHPEGRGAI
                     ACMKQAIAEAGINPSDIGYINAHGTATNKGDTVETSSIKKVFGNTLPYVSSTKGATGH
                     MMGAGGITETITCVKAIETGTVPPTINLNEVDPECAGIDFVANTAKKAEINFAMSNAF
                     GFGGQNSSIIVGKYGK"
     gene            23921..24832
                     /locus_tag="PLS64_00310"
     CDS             23921..24832
                     /locus_tag="PLS64_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL42307.1"
                     /translation="MDINQLNCFITVAQTLNFSEAARRNYVSQSTVSRYVSDLEKEFG
                     VQLFTRSHRDVIITSEGKALLPYAIEIVETLKKAKTVIKQMHDGGQGKITLGCDVTSL
                     SFPTKCIADFAEKYPGITIDVRQLDEADRTLAITGGEYDFCFMPRDMVPESSGIESII
                     THNETLSVVTAKNSKFSGRKSVKLSELADESILVLSESVAPIVYMEIMDLFRTFHITP
                     KIENSFDDLVSIYVSSSSGIGVSVIPTSVAAYTSDEYTDSYLIDDADTSIAYVMAWEK
                     NISNPAAKLFMEAVKKYAKGDDNIYGL"
     gene            24999..26210
                     /locus_tag="PLS64_00315"
     CDS             24999..26210
                     /locus_tag="PLS64_00315"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRL42308.1"
                     /translation="MYRFTLPRDLYHGKDALSSLKNLSGKKAVVVVGGGSMKRFGFLD
                     KAVDYLKEAGMEVKLIEGVEPDPSVETVMKGAAVMREFEPDWIVAMGGGSPIDAAKAM
                     WVFYEYPNTTFEEIITPFSFPTLRTKAKFCAIPSTSGTATEVTAFSVITDYAKGIKYP
                     LADFNITPDVAIVDPALAETMPAKLTAHTGMDAMTHAIEAYVSTLNCEYTDPLALHAI
                     ELIHDNLKKSYEGDMACRDKMHDAQCLAGMAFSNALLGIVHSMAHKTGAAFTGGHIIH
                     GCANAMYLPKVIKYNSKDPVAAERYAKIAKFINLKGETTEELVDALIAELRSMNDQLN
                     IPQGIKNYGPGGVKADESIIDEKEFLEKLPEVAKNAIADACTGSNPRQPNQEEMEKLL
                     KACYYDTEIDF"
     gene            26380..27075
                     /locus_tag="PLS64_00320"
     CDS             26380..27075
                     /locus_tag="PLS64_00320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRL42309.1"
                     /translation="MKIAVINGQNHKGSTYHIGKQVADKLGGEVTEFFLPRDFGDFCV
                     GCTQCFLKSETLCPHYERLKKITEAMDEADVLIFTTPVYVYHCTGSMKAFLDHYGYRW
                     MVHRPEQKMFTKQAVVISTAAGSGTKSANKDIADSMFFWGVPKTYRYGVNVRATNYSG
                     VTDELKKKIDKKTTSIANKVKKNNGKVKAGLKTKGFFFIMRMIQKSGWNEADKNYWAE
                     RGWNGKNRPWKNT"
     gene            27089..28372
                     /locus_tag="PLS64_00325"
     CDS             27089..28372
                     /locus_tag="PLS64_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL42310.1"
                     /translation="MAKNTEISLRNKVIYSVYVRNHTNDGTFNAVAEDLDRIKSLGTD
                     IIWFMPIHPIGVKNKKGSLGCPYANKDYRTTNPEYGTMDDFKSLVEKIHAKGMKCIID
                     VVYNHTSPDSNLSVEHPEFFYRKPDGNFGNKVGDWSDIIDLDYSNKELWQYQIDSLVM
                     WAKIVDGFRCDVASFVPVEFWKQAREAVAKVNPDCIWLAESVHSSFNVFSRKSGIYTA
                     SDYELFDAFDMEYDYDIREVFDKYLKGETSLSNYMDMFNYQEAIYPQNYDKMRCLENH
                     DQPRICHYVKNRSDLENYTAFLYFLKGSTLIYAGQEFGCDETPSLFDKDVFPRNTGID
                     LSNLFAKLDTIKKTVLDDDDYFKADADDENDIAILERDNNKSKKVGIFSLKSKSADVK
                     VDLPDGDYKNEISGETVTVSNGKVHCNGKAIIIRK"
     gene            complement(28581..29831)
                     /locus_tag="PLS64_00330"
     CDS             complement(28581..29831)
                     /locus_tag="PLS64_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRL42311.1"
                     /translation="MTKRTVANNFLYYIFCGFSGAVTSLIVWLFLKLMGLGIGLVWDT
                     IPSNFDFKFYPIIVCTAGGIILGIWQKLTNAVPDELDEVMKKVKKDKFYPYNKVLLLC
                     ISALLPLVFGGSLGPEAGLTGVIVGLCYWAGSHMKDAKSKIPELMQIGISATLSAVFY
                     APLFGLASVNEERLDSEEKPTDIRSTKLISNIIAVLFAAGTLFLLNSIFGGSMGLPRL
                     GGYNITNFERLWGIPLALLGAVCGFLFIISSKIFGKFFALIQGKLGIIISTTLGGIIL
                     GVMGTYLPLTMFSGEESITVVKESFAEFAPWILIISGVLKLVLTNICIKSGWKGGHFF
                     PVIFCGVSIGYGVAMLGGLDTAFCAAVVTAGLLGVTMKKPLAVTMLLLLCFDARVIPW
                     ILLAAFLGSIIPTKIFGNNKKKAK"
     gene            complement(30009..31460)
                     /locus_tag="PLS64_00335"
     CDS             complement(30009..31460)
                     /locus_tag="PLS64_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL42312.1"
                     /translation="MEKVKKMLSLAFASLFRRNKGQNTVKEKRRRSHQINMLEGPLGK
                     KILLFTLPLAGCSILQQLFNSTDVAVVGRFSSSQALAAVGSNGPLINMLVLLFTGLSV
                     GANVLIARYIGQNDRKKVSEAVHTVITLSLICGFLLLILGQVIAAPLLRLMNTPDDVI
                     DLAATYLRIYFMGMPFVMLYNFGSAILRSVGDTSRPLYCLVISGIVNVLLNLFFVIVC
                     DMSVAGVGIATVIADGISAGLVMMFLIRNEDESIRVNPKKLSFKKEHLIMILKIGAPA
                     GIQGMVFSISNVFIQTAINGFGSQAVAGSSAELNFEYITYYIVCAFSQTAVTFTSQNY
                     GALQFDRCKKVFRLCMLFSCIFTGCVSVIFMLGSGFFITFFTTDPTVIKYAIIRMAIV
                     MLLELMTGSYEISGGALRGIGHSLLPAILTIFGSVVFRIIWLFTVFKAFHTYDMLLMV
                     YPVSWLITGTSVLIAYFIIRKREFKKVSTAFEI"
     gene            31903..32295
                     /locus_tag="PLS64_00340"
     CDS             31903..32295
                     /locus_tag="PLS64_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42313.1"
                     /translation="MKKKIAVIVVAVVLCVAAAVFAVPKISFYACEPTVYFDVEYCDK
                     VDAKMSAEDAETVKKMFEGKSAYFDSPSCGFSEKASIRIGCNTYMPACDGDEIVKHGF
                     MYFSLSKSENNELRKIMKKYGADTRKAI"
     gene            32359..33333
                     /locus_tag="PLS64_00345"
     CDS             32359..33333
                     /locus_tag="PLS64_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRL42314.1"
                     /translation="MKNYNWATLGCGVIANELAAALKSRGQKLYSVANRTHEKAVAFA
                     EKYGIEKVYNDIDELFKDENVDIIYISTPHNTHIKYLRKALAAGKHVLCEKSITLNSD
                     ELDEAISLAEKNHVVLAEAMTIFHMPIYKALLEKVNSGDLGELRLIQMNFGSYKEYNM
                     NNRFFNRNLAGGAMLDIGVYALSFVRMFMSSCPNDVLSQVKYAETGVDEQASILLKNK
                     EEEMATVILSLHAKQPKRGTISFDKGYVEIFEYPRGMEAKITYTADGHCETISAGQTK
                     DALLYEVEDMEKAIEGDTALMHLDYTKDVMKIMTDIRKSWGMKYPEEE"
BASE COUNT         9610 a         6833 c         7306 g         9678 t
ORIGIN      
        1 ctggctgata attttaagcc tgtttttccc cgcagatatg cctgcgttcc ctgtatttac
       61 acccggctca aattgcagat gggcgtctgt tgcggcatgg ttgccgcttg tcggaatgac
      121 gggagttatc gcatattttc aggtttgccg aaaaagctgg ctcaaaaagc ttcttgccgt
      181 gcttgctgtg tttgcgtgtg tgcctgtttt gaacagtatg ttccagctta tgaacagctc
      241 aatttactac gcaagatggt tctatatggg cgtgcttatg cttgttcttg caacaatcaa
      301 ggcgtttgaa aacagaaaaa ccgattggaa cagggcgatt cgttggtcgg caggaattac
      361 ggtaggcgca actctgctga taggtcttat gcctgtgagc tatacggatg aggagagcgg
      421 cgatattcaa aatacggtta tcggcactca ggcaacattt gaacgctatt ggctctatgt
      481 tcttatggct ttgttaagtc tgcttgcgtt cgttctcatc atcaaaaaat tcagacgaaa
      541 caaaaagaga tttacggtta tgctcactgt tggtatcctg agcgtttcgc ttttcacctc
      601 atattttata atcgccacag gctatttctc aagcagcagt acaaatacca tcaaagaaga
      661 tatcataaac aggcgtgacg gtatcacaat tgccgatatt gacaatgttc gcagtgattt
      721 ttacgaatgt gttgacaaca cggcaatgtt ctggcagatt cagagcatta actgctttca
      781 gagttctgtt tcaacatcta taatgcagtt ttacgatgca ctcggaatta caagggatgt
      841 tgcgtcaaga cctgaccttg atgtatacgg acttcgcccg tttctgagtt gtaagtatct
      901 ttttgattac cgtggcgacg gaaaatcggg aagtctgaat tcattcgttg acgaaaacgg
      961 caacacaaga atgccgggtt ggaaatatct cagaacgcag aaccgttttg atatttaccg
     1021 caacgaatac tacattccta tgggatacac ctttgataag ttcattgccg aagaagaatt
     1081 tgaccttgtg acgaacgcac acaagtccga ggctttgctc tacgcaatgg ttcttccccg
     1141 tgaccttatg aagaagtatt ccgatattac gggctacagc gatgaaaagt acaagctttt
     1201 gtacggcaaa catccggagg attacgacag cattacggaa aagtttgatt acagtaattc
     1261 agattacaaa aaggtttgta atctcagagc cttgaacagt tgtacttcat ttgaatatac
     1321 agataacggc tttaaggctg tttacaataa taaaaagggt gacgataacc tgttgttttt
     1381 cagcgttcct tacagcgatg gttttaccgc aactgtaaac ggcaaggcgg cagatgttga
     1441 aaaggttgac tacggcttta tggctgttaa gattccaaag ggcgaaaagt gtgacattgt
     1501 gttcacctac gaaacacccg gattttcaac aggtgtcaaa atcagccttt gcgcattcgg
     1561 agcattttta atctactgtg cggttattgt gaccgttaaa acagttaaaa aaagaaaaaa
     1621 taaaaactga taaagataca atcagcattt aagtacggag gtatttatta tgtttggaaa
     1681 caaaatactt gacttcaaag acgaactttt aaaggatctt aacacacttc tttcttttga
     1741 atcggttgac ggtgaaaaga atgacgagtg cgataatgcc ttgaatttta ttcttaaaag
     1801 agcagaagat ttcggactga caggggagag aacaaccgac aagagcggtc atataaccct
     1861 tggcgacagc ggaaagcttt gcggtgtgct tactcacctt gatgttgttc ctgcgggaaa
     1921 cagctggagc gttccgccgt atgcgcttac cgaaaaggac ggcagacttt acggcagagg
     1981 aattgcagac gacaagggtg cggctcttgt tactctctat tgtttgaaag caatcaagga
     2041 cagcggtgta aagggcgtga acactctgcg tgctatttac ggcacgggtg aagaaacggg
     2101 tatggaggat atggaaaatt atttcaagaa aaatccactt cccgacatgg cattcactcc
     2161 cgacagcgac tacggcattt gctttgccga aaagggtatt cttcagcttg aagtcagcac
     2221 acttttaaat aatgcaacaa cactcagcca gttccatgca ggcagagcag ttaatgcagt
     2281 tcccgacaga gcgtatgtaa tgcttgattc atcagactat gacgagcaga ctctcatgcg
     2341 ccttgcagac gcaagtgacg gtgattttga atttaattac actattgacg gtcttatgat
     2401 aatcagccgt ggcaaagcgg ctcatgcgtg tgaaccggac aagggctaca atgccgcagc
     2461 ggcacttgtt gaccttatct caaatgttta cacaacaaag gaaacaggtt caatctgttc
     2521 atttattgac tacgcaatca acaaggaaac aaacggcaga tcactcggtc ttaaaatgag
     2581 cgacgctgtt tcgggctcgc tcaccgtaaa cctcagcagc gtgaatattg agggacagac
     2641 cgcaaaggct gtgtttgata tccgttatcc cgttaccgtg agcgtaaaca gagttctcaa
     2701 acagttcaaa aaggttgcaa aaatcagcga cctcaaggtt actgttctca atcacgaaga
     2761 accgctttat gccgataagg acagcaagct tgtaaagctt ctctctgacg cttatgaaag
     2821 cgtaacaggt gaaaaggctg agctttattc aacaggcggc ggaacatacg caagaatgct
     2881 cggaggtcac ggtgttgcat tcggtcctgc attcaaaaat gatgatgttc gtatgcacaa
     2941 tgcagacgaa agtatggata aggagaactt ctttaaacac gctcagattt gccttgaggc
     3001 aatgtacaga atgtttactg acggagaata atgcgtttcg ttatgaacaa attcagatga
     3061 aaagggaatg ctaatgaata cggaaaaaac tgttagaaaa caggcaaaaa aggttcttga
     3121 ggggaaccgg tcggtaatta tctcagagat aatggttgct gtgcttgcat ttttgacagg
     3181 gctgtttgcg ttcagccttg caatgtctgt ggcaggactt tatgatgtaa aaaatccgaa
     3241 tcagactcag cagatgctta caatgatttt cggacttgta ttttttgctt ttgttgttgt
     3301 gtgtctgccg ctcataaacg gagtttacag aagtgtgtgt aatgtagtca ggggcagaga
     3361 gtgcagtcct cttgatgttt tttactatta taaaaagccg aaattatttt ttaagtctgt
     3421 tattcttgat gtaatttcag tcggcttgtt ttttatcata agcggtctgc tcaatgtgtt
     3481 caattatctt agtgcggttt cggataaaat aattgacaat tcaccctcac tcacggcggt
     3541 tgtggctgta ttgcttgttt ttgcgtttat cgtcagcact gcggttgttg tggtgtgtta
     3601 cattattttt gttcattata catttttggc ttacggtttt aacgaacatc tgccgctcgg
     3661 aatttatctt ccgagaatga tggctttttc aataaagaac ttcatctcca caataaagct
     3721 gtttatcggt tttatcggct gggcggcttt gtgctttttt gttgttccgg cattttatgc
     3781 cgtaccttat tttctgacaa cctctgcaat gtctgcaaaa tggttgttcg agcttgaagg
     3841 gagcagagat attatatgtt agtttcactt tgtatgatag cctataacga atcgaatgct
     3901 ttgagcggac ttttcaggga tattgcactt caggactatc cgcatgataa aattgaggtg
     3961 gtatttgttg acagtatgtc aaccgatgat actaaagaga aaatggaaaa attccgtgat
     4021 accgactacg gcttttggaa tgtgtccgtt gttcaatgcg taaagcgtaa tcaggcaacc
     4081 tcatggaatg ccgctctgat gaccgcaaag ggcgatgtta tcataagagt tgacgctcac
     4141 gcaagaattc cgagaaactt tgtttcacga aatgtttata acataaagca gggcgaaaat
     4201 gttgtaggcg gcggcagacc gaatatcact tcaaatgttt caagctggaa gcttaccttg
     4261 cttgcggctg aggattctct gttcggcagt tcggttgctt cgtacagacg gccgcttgcg
     4321 caaaaggaat atcttgacag cctttttcat gcggcttacc gcagagaggt tattgccaag
     4381 gtcggcggat ttaacgaaaa tctcggcaga accgaggata atgaatttca ctaccgcatt
     4441 agaatggcgg gctacaagat gtgctgttgc cccgatatta tttcgtatca gcattcacga
     4501 aacgaccttc acggaatgat aaggcagaag tattcaaacg gacggtggat aggacttaca
     4561 ctctcggaat gtcccggttg tctttcatac tttcactttg caccgtttct ttttgtaatg
     4621 gcacttctcg gctgttccgt gcttgcattt ctcggacttc cgcttttttt gtacattctt
     4681 ttgataatct acggaatgtt tgatattgta aacgctgtcg gatgttgcac aatgaaaaat
     4741 gttcagcctc agtttgtgtt tttgccgttc attttcccgt tgctccatgt tgcctacgga
     4801 ataggcacaa ttgttggttt gattcagatt ccgtcatggc ggaagaaaat caaaaacagc
     4861 ggtgcgaaag aacacattga aaaggtaaaa agaaaaatca gggaaaacac acttcccggt
     4921 cattcataca aataattttt tgggcggata atctccgccc gtatttgata tttacggaga
     4981 tacttatgac tgaaaaagta gaacttaccc ctgaactttt gcgtgagtta cagcttaaac
     5041 agcttgatat gctcgtgtat ttcagggact tttgcgaaaa gaacaatctc acattctatc
     5101 tcatcggcgg tgcgttaatc ggtgcgctca gaaacggcgg attcgttccg tgggatgatg
     5161 atgttgatgt tatgctgcca cgagaggact acgaaaagct gtacaagctt tggcatgaac
     5221 agggcgcaga cggaagattt cgacttctca aaaccgacag cgatatgttc acgggcaata
     5281 tttttatgac cgtaaccgac acaaattaca ctatggtcaa gacaaatcag accgaagttg
     5341 atattccgca cggacttgtg cttgatgtct ttccgcttga tgtttgtccc gacagccgtt
     5401 ttgcacgcaa aatgcagtat gtgtggacaa tgctttattc gctgtttttg gctcaggttg
     5461 ttcccgaaaa gcacggcgga attgtggggg caggcagtaa ggctttgctt tcaattttca
     5521 gaggaaaaaa gctccgagaa aagatttgga gaactgccga aaagcatatg attaagtaca
     5581 ggcttgataa aaacaagtgt gtaacggagc tttgttcggg ccctcattgg atgaaaattg
     5641 agtatccgaa acacatttat tcgggcgttg attatgtcac atttgagggc ataaaaatgc
     5701 cgtgtatgtc gggctatgac gagtatctca gaatggtttt cggtgattat atgcagcttc
     5761 cgccggttga ggacagagtg ccacaccacg atattgcata tcttgacctg aactcaccgt
     5821 gtgaaattta tgacagcaaa agaaagaata agcaaaaata acggtgcatt atggagctta
     5881 taagagataa ttggactaaa aatgactatg atgattttgt agaggaactt cgttcatatg
     5941 ccgatgaaaa gtacagaagc tttcattcct cattgattcc cgacaatgac aaggactttt
     6001 ttatcggagt aagaatgcct gttatgcgaa agctcggcaa ggaaatttct aagggcaacg
     6061 gacgcagttt tttagagcag acaactgata attcttatga agaaaaaaca atgcgaggta
     6121 ttgtaatcgg tttgctcaaa accgaaagct atgaagaatt tttgatgtac agcgatgatt
     6181 ttgtacagcg tatagacagc tgggcggttt gcgacggctt ttgtacaaat ttaaagcaga
     6241 cgaaaaagta caaatcagaa ttttttcctc nnnnnnnnnn aaaacggcgc ctgccgcatc
     6301 gaactgaagg tgctatcatg caacaaagtc gctctgcgcc tttatgagaa actctccttc
     6361 aaagaaacca aaaagtataa atcagaattt ttccctcatg tttgtgaata caccaaaagc
     6421 gacaatccgt ggataattcg tgcaggtctt gtgctgatgc tcgactatta tcttgaagat
     6481 gagtacatag acacggtgct taaatgttgt gacaattcga aaaatccgca ttattatgtg
     6541 agtatggcaa gggcatggct tattgcaacg gcacttgcaa agtgcagaga acagactatg
     6601 cggtacataa agtcaaattc gcttgataat gcaaccttta ataaaatgat tcggaaatgc
     6661 gttgaaagtt tcagaattga cgatgaaacg aaaaaatatc tcaaaacact gaagagatat
     6721 tgacaaatcc gaaaatcggc atataatata atcaacagtt gtcttcgggg cggagtgaaa
     6781 ttctccaccg gtggttaaag tccacgaaca ggtgcaagcc tgccgagtct gtgaaattca
     6841 gacaccgacg gtcatagtcc ggatacgaga agatgcggca gaacaggcgg ttcgtctgtt
     6901 ttattttggt acgcaatccc gttacatttg tagcgggatt tttcttttcc cgacaactcg
     6961 aaataatttt tcaggagtgg tcgtaatgac aaaatcaaaa gcaaaaacaa tcaattcaga
     7021 caacacggca aagacaacaa agcttgccgt aaccgcaatg cttgcggcgc tttcggcagt
     7081 tcttatgttc tttgaatttc ctctcggatt catcgcacct tcattctatg agttcgattt
     7141 ttccgaagtt cccgttcttg tcggaacatt cagcatgggg ccgattgccg gtgcagtaat
     7201 tgaatttgta aaaattctcg ttaagttcct tatcaaaggc acaagcacag gcggagtagg
     7261 ggagcttgca aacttcctca tcggctgttc gtttattctg ccggcaggtt tcatctacaa
     7321 gtacaaaaaa acaagagtcg gtgcgattgt cggaatgctt acgggtacgg ttgcaatggc
     7381 ggctatcggt gtggttctca acacattcgt gcttgttccg ctctactcgt catttatgcc
     7441 gcttacagaa attatcaaaa tgggacaggc aattttccct gcaattgacg gcacatttac
     7501 attctgcctg tactgcgtag gtccattcaa catcattaag ggactcatca tttcggtagt
     7561 tgttttcata atctacaagc cgctttcaag acttatcaac agcctcgatg cccttcttac
     7621 aaagaagaaa aaagcaacag ttcagtaaac ccgaaattaa tttttggttg acaaacagca
     7681 aattcaggcg tataatagca tacggtgtcg aaaggcatct atcacagagt agaaatttgt
     7741 gcgtgaagtg ttttgcggac ggggagttgc cgcaagaacg aaaggtttat cctgcggtac
     7801 agatttcgca tcccgctgtt actttgggac atataattca gcgggcagag caatctgtcc
     7861 gcttttttct tcctgaagca acaacagaga ttcaggaggt aaaaaatatg tcacaactca
     7921 aaaaaattca aagctctttg cttgaaatga agagtatcta cgctcttgcg gcaattgcaa
     7981 tgctgcttgc acttcgtatt gttctcggat tttttgcaaa cagcacactt ccgtttttcg
     8041 gaaacaatgt aaagctcagc gcagcgttcc ttcccatagc cgtaacaggc gtaatgttcg
     8101 gaccgattcc tgccgcaatt gtcggtgctt tgggagatat tctttcattt atcattgccc
     8161 cgacaggcgc atattttccc ggctttacaa tcaacggctt tattacaggt tttatctacg
     8221 gtatgtttct ttataagaac aatgtaactc cgctcagagt tgttcttgca tggtttacaa
     8281 atatgctctg cgttgaaaca ttccttgcgg ctttttggct ttacacactc tacggcggcg
     8341 gttcaacacc gtacacagtt tatcttacag caagattcat cagtgttgcc gttaagtgca
     8401 ttcccgagat tattcttgtg tttgctcttg gtaagcttgc aggtaaaatt cgtgttccgc
     8461 acgcaataaa gagaacctga tgaaaaacta atgcaaaatt ttaaggttgg aaatcaactg
     8521 ctccccgcag tagtttccaa ccttttttgt ttgtgtagcg gcggatagta acggtacaat
     8581 atttgtaatt caacttgctt tataaataca acaatgtcat tgttaaactg tagaggcgaa
     8641 ccgtgttcgc ctctacaaat aaaataaaag tcggaaagca aatttgaaat ctgctttccg
     8701 actgaatttt ttgttataaa atttgactgc acgattaagc ccacttgagg gtatcgttgt
     8761 agagattgat gtagtcctga gccgaacgat cccaactgtt gtcgctcatc atagcacgcc
     8821 acataagctg atgccaaccc tggtcgtcct gaatgcccca caatgctcgt cttacggcaa
     8881 gctgcatatc tgcacattca tagtttttga atgtaaatcc attaccgtag ttgtcggcac
     8941 tgtcgaaaac agagtcttta agaccgccaa cctcacgaac aacaggaatt gtgccgtaac
     9001 ggagtgcaat catctgtgca agaccgcaag gctcctgagc agaaggcata aggaaaatat
     9061 ctgcgcctga gtaaatcttt cttgcaagtt cgggaacaaa gccgtgacag aagcaaagtc
     9121 tgcccggata cttcgcctgc atatagttga agtagtcctc gtactccttg tcacctgaac
     9181 cgagaattac aaactgcata ttttctgtgt ccataagctc ctcaaggccc attcttacaa
     9241 ggtcaagtcc cttgtgtgca acaagtcttg ttaccatacc gaccagcgga acatcccagc
     9301 gctgttcaag acacaaacgc tcctgcaaag cacgcttaca ttcgcccttg ccgctcatat
     9361 cgtcctttga atagttgcga taaagctgat agtctgtttc gggattgtag ctttctgtgt
     9421 caataccgtt cttgatacca caaagcttgt aatgacgaag attgagttcg tcatgaagtc
     9481 cccaactgaa ccacgggtct ttaatctcag ctgcatagct tggagaaaca gttgaaacac
     9541 gggtagctgt ttcgatagca cccttcatca tattgagttt gccgttcatt tcaagaatct
     9601 ttgtttcgga tgcaggaatg ccgacgcact ctgtgttaac ttcccagccg tattcaccct
     9661 gatactggat gttgtgaata gtgaacagac tcttaatgtt atagtaccat ggtctgtgtg
     9721 aatagaacag atagtagtaa acaggaacaa gagccgtctg ccagtcatta caatgaatga
     9781 tgtcgggatg gaaatcaatg taaggaagca tttcaagaat tgcacgggag aagaaagcga
     9841 atctttcagc gtcgtcataa tgaccgtaga gagtgcctct cttgaaataa tattcgttat
     9901 caataaaata gtatgtacag tcattccatc ttgcccagaa caaaccgcaa tactgatgac
     9961 gccatgcaac cggcacggta aagtttgtga tgaagttcat ggagttttta agttcctgcg
    10021 gaattgtgcc gtaaagcgga acaacaattc tgcaatcctg accttttctg caaagtgtgt
    10081 gcggaagact tcctgctaca tcagcaagtc cgccgctgcc gcagaaagga ttagcctctg
    10141 aagcacagta taaaattctc attgtaagat tacctcctgt ttaaacaact ttatacaaca
    10201 gattttttag caatataaat ggggtacgaa tcatatccca taagtgatcg accatccttg
    10261 atggtaacat ccttatctat gataacatag ttgaggttgg tattgcaacc tatttttgtg
    10321 tcctgcatta caatacagtt agaaagcttt gcgccttcgc cgacataaac accctttgaa
    10381 ataacgcagt tctcaacctc accgttgata acacaaccct gtgcaatgat tgaatttttc
    10441 acattgcttg taagtccgta gcgtgacgga gcgtcatctc ttacctttgt atagataggt
    10501 ctttgcggat tgaaaagctc atcccttact tcttttttca taagatccat attgaactca
    10561 aagtaatcgt tgattgagct gattgccgca cagtaaccct tattttcata agcataaata
    10621 ctgtatttgc ccagactgct ctgaataagt cgccttacat caagctgatt aacgctgatg
    10681 gcgtttcgga tttcactcat aagaaggtct ttcttgatga gaatagagcc gtatgcgtgg
    10741 ttgaataccc ctttgtcaac aacaccgggc tttacaaaaa tctgatttac cttgccgtca
    10801 ttgtctacat caagggtaat cggtctgtca tagccttgcg gaattgcatg attcttatat
    10861 ataagtgtaa tgtctgcctt cttctttgag tgaaagtcaa aaacatctgt gtaatcaatg
    10921 ttagtaatta cattgccctg tgaaataagc acatactcct cacggcaatg ctcaatgtag
    10981 ccgtgcatat tgtagattgt ttcaacaaag cttgaaaaag ccttgccgtc atatggtggg
    11041 agaattgaaa gattgctcct tctctttgaa aggtcatatg cactgcccga acccacatga
    11101 tccataagtg actgaaagtt gctttttgca actatgccta cattgctgat acctgagttt
    11161 gacatatttg aaaggtcaaa gtcgataatt ctgtattttc cgccgatcgg aacgcttgca
    11221 gttgttcttg aagatgcaag ctcggggatt ttgtcgtcaa gtgttgccgc aaagatgaaa
    11281 ccgagtacat tattacttct cattgccgtc cacctccgtg tcgctgtcta tcatggtttt
    11341 tgctttgata actgtgtttt ctttgataac ggtatttgca ccgacaacag taattcccca
    11401 gtcgtcaata ttttccgttt cttcggggtt ttgtccgact tttgcatttt tgcctacaac
    11461 ggtgttttcg gcaatgatag caaactgaac ctcggcaccg tcctcaatta ccgcaccggg
    11521 catgatgatt gaagaattaa tcgtagcatt tttaccgatt gtaacacctg caaagagaat
    11581 tgagaactca aggttgccgt aaacattgca tccgtcggca acgagtgaat tttggattga
    11641 cgcctcgttt gaaacatagt gcggaggcat catcgggttt cttgagtaga tatccttgag
    11701 gtctaaagta acctttggat cgagaagatc catattagcc tcccaaaggc tgtcgattgt
    11761 tcccacatcc ttccaatagc cttcaaaagg atatgcaaac atacgctcac ctgcgtcaag
    11821 catggcaggg aggacatcct taccgaaatc gtggcttgaa ccctctttga attcatcctc
    11881 cgtaagatac tttttaagtt ccttccagct gaaaatatag atgcccattg aagcgttagt
    11941 gctttttgga tgagcaggct tttcttcaaa ttcgtagatt gagccgtcct catttgtgtt
    12001 gagaataccg aatcttgagg cttcttcaag cgaaacatca attactgcaa ttgtacaagc
    12061 ggcattcttt tccttatgat attcaagcat cttgttgtag tccattttgt agatatggtc
    12121 gcccgaaagg atgaccacat attcaggatt ataacgctca atgtagttga tattctgata
    12181 aattgcattg gctgttcccg aataccagtc tgcaccgtca acagcctgat aagggctgag
    12241 acagtttaca cccgagtgca tactgtcaag atcccacggc tgtccgtttc ctatgtattc
    12301 gttaagaaca agaggctgat actgtgtgag aatacctaca gcgtcaatgc ctgaatttac
    12361 acagttagat aacgggaaat caataatacg atatttgccg ccaaacggta cggcaggctt
    12421 tgcaagcttt ttggtaagca cgccaagacg agagccttga ccgccggcaa gcagcatagc
    12481 cactacttcc ttttttggaa acatcttaaa atacctccga tatttatttt attatcttta
    12541 ttaaacttat taattacagt cttgtgactg aaaatatcaa ttttctttct gagcttcatc
    12601 gggttcaagt tcttttgcac acctcagata gattacgctg agagggggga gagtgagtga
    12661 aagtccctga tcaaaaccgt gaattttcat tacctcggtt ttgatattat taccgtttac
    12721 aacaccgctt ccgccgaaac gctcttcatc agagttgaaa acctcgtcat aaagaccgta
    12781 tttaggaaca ccgatgcagt attcgtcacg cctgatgggc tgaaaattac ataccgaaac
    12841 aatttcatca ccgtttttgt cggtgcgctt gaatgcaata acactgtttg tgtaatcgtc
    12901 atggtgaatc cagtcaaagc ctttccagac cgtgtcaagt tcgtagagcg gcttgcagtc
    12961 aagataaaat ttgttgagct ctttaaaaaa tgttctgagc tttttatgct tttcaaattc
    13021 aagcaaatcc cattgaattg cctcttcgtg attccactcg tcaaactgtc cgatttcagt
    13081 tcccatgaac acgagtttct tgcccggatg agcgtacata tatgttatga atgctctcac
    13141 gcctgcaaac tgctcgtcat actttccggg catctgcttt atgagtgagc ctttgccgtg
    13201 agaaacctcg tcgtgtgaaa tcggcagaag aaatttttct gaaaaagcat aataaaagct
    13261 gaaagtaaga ctgtcatgat tgaacggccg ccacatcgaa ttgagtgaca tatagtgcag
    13321 catatcgttc atccagccca tgttccactt gaagtcgaaa ccaagaccgc cgtcctctat
    13381 tttatgagta agctttggcc acgatgtgtt ttcctcggca aacatcataa catcggggtg
    13441 gtacatatga acagccgtgt tgagtctttt tacaaaatca acagcgtcaa gattttcttt
    13501 tccgccgaat ttgttcggct cccattcacc ctcggtttta ccgtaatcaa ggtagagcat
    13561 agaggagagt gcgccgattc tcaagccgtc aatatgatat ttgtcgagcc agaacattgc
    13621 gcatgaaaca agaaaacttg taacctcgaa tcttgcgtag ttgaaaaggc aggttcccca
    13681 tgagtcacgc ttgcttgttt ttggatcgtt gctttcataa agaggtgaac cgtcaaacct
    13741 tgcaagtccg aaatcgtccg tcggaaaatt tgacggaacc cagtcaagaa tcacaccaat
    13801 gccttcaccg tgaagcttgt caacaaaagc cataaaatca gacggtgtgc catagcgtga
    13861 agtcggggca tagtagccgg ttgtctgaaa accccagctg ccgtcatagg gatactccat
    13921 aatcggcata agctgaacat gggtgtagtg catctccttt aggtagggga taagctcatc
    13981 ggcaagcttt tgatagttaa aaaagttgcc gtcaggatat tttctccatg agcctgcatg
    14041 tatttcataa atattcatcg gtgacgatga aatgttgttt tctttgcgtt tattaaacca
    14101 ttcgctgtca ttccacgaat agctttctat atcatataca acagaggcct gaccggggcg
    14161 ggtttgagca tgaaaagcat acggatctgc tttcataagc ctgtcgccgt attcgctttc
    14221 aatgcagtat ttgtatgtac aaaactcttt tacaccctcg acaaacactt cccacacacc
    14281 gtaaccgatg ttatacatat agtttgcctc gttgttccaa ttattaaaat cgcctgcaac
    14341 cgaaacgctc cttgcggttg gcgcccacac acggaacacc actccgtttt tgtcgttacg
    14401 gttcacgagg tgtgcgccca aaaaatcata tgcacgcact gaatcgccgg tgtgaaaaac
    14461 ttcaagagcc ggtcttgcct cgttaaagct gtaattcata agaattgctc cttgtttaat
    14521 atctgttgat tgttgattgt ttgctcacac ttatataata ccaaaattat atgtcaattt
    14581 caagcaaatt caaaaaattt aaaactaatt tttcgtgtat attttataaa ctgcgactaa
    14641 atgttacatt ttaaagcata attttacgaa taaagaaaaa taagaataaa tatcttccga
    14701 aaaggaaaac aaaaagggct gtccaaaaag gacagcccaa agaataatct gtaaattcag
    14761 attagccaag ctcagcgaaa tacttaattg ttcttaccat ctggcttgtg tatgagttct
    14821 cgttgtcgta ccaagaaaca acctgaacct cataaagatc atcagcaatc tttgatacca
    14881 ttgtctgtgt agcatcgaag agtgaaccga atctcatacc gattacatct gaagaaacga
    14941 tcggatcttc gttgtaaccg aatgactcag aagcagcagc cttcatagca gcgttgatac
    15001 cttcaactgt tacatcagaa cccttaacaa cagctgtaag aattgttgtt gaacctgtcg
    15061 gaacaggaac acgctgtgca gaaccgatga gcttaccgtt gagctcagga attacaagac
    15121 cgatagcctt tgcagcacct gttgagttag gaacgatgtt agcagcacct gctcttgctc
    15181 ttctcttgtc gccctttctg tgaggaccgt caagaatcat ctgatcgcct gtgtaagcgt
    15241 ggattgttga catgataccg ctctggattt ctgcatactt gttaagtgtg tcagccatag
    15301 gagcgaggca gtttgttgta catgaagcag ctgaaatgat tgtatcgtca gctgtaagag
    15361 tcttttcgtt tacgctgtaa acgattgtct tgaggtcgtt gcctgcagga gcagaaataa
    15421 caaccttctt agcgccggca tcaatgtgag cctggctctt tgcctttgat gtatagaaac
    15481 ctgtgcactc gagaactaca tcaacaccga gttcgcccca aggaagctca gcagcgtttg
    15541 gcttagcgta gattgtgatc tctttgccgt caacgatgat tgagccttcg ccggcctcaa
    15601 ctgtgtgaag gttttcaccg atcttaccgc agtagccgcc ctgtgctgta tcgtacttga
    15661 gaaggtttgc gagcatagca ggatctgtaa gatcgttgat agctactact tcgtaaccct
    15721 cagcaccgaa catctgtctg aaagcaagac gaccgatacg gccgaaaccg ttaattgcaa
    15781 cttttactga cataaaaaaa ttcctcctaa aattctaagg tgcttctatt atattacatt
    15841 tttttcattt cttcaatagc ttgacaaaaa tttaacgaaa aatttttgag ttttttacaa
    15901 atacggtaaa aaacacggtc acattttgct tttttaaggc aaatttaagc tgtaaccgtc
    15961 aaaaagctgt aatctttttt atttccgtaa taattatctg caaaattccc gtgcttattc
    16021 ttgacaattt ggttatttta ttataaaatt gtatagtccg ctgatgcgga ttttggcaaa
    16081 agtacagttt ttgacttttg cgactttttt aatcagactc tttaaaagag ccgttatata
    16141 tagatatttt ttataatttt tatgacgttt cgccttttga aacgctctgt gcaacctgta
    16201 cggtgttttg cggttgcgta tgtatatata atgtattaaa aatatttaca aagtaaaaat
    16261 gaaaattgaa tatcttttta acggcacctt taaagtctaa ttgattggga actgtgaaat
    16321 attaaaaaat caaactaagg aggtgtccag attgccattg tggataagta ttatctgcat
    16381 tgtcgccgca gttgttatcg gtgccggtgc aggtattttc cttggaattg ccatccgcaa
    16441 aaacacagcc gagaaagaaa tcggaagtgc tgaggaggag gcaaagaaga ttgtggctga
    16501 tgcaatcaaa acagccgaag caaggaaaaa agaagtagtc cttgagggta aagacgaggt
    16561 tcatcgtttc agaaacgaga gcgagaaaga aatttccgat cgacgcaagg aagttcagcg
    16621 tcaggagaga agaatccagc aaaaggaaga aactcttgac aaaaagcttg acaatatgga
    16681 acgcaaggaa gaaaaggtca acaaaaagct tcgtgatgca gatgcaaaac tcgaagaagt
    16741 tgaaactctg aaaaagagcc agtttgaaat gcttgaaaaa atttcgggct acacggcaga
    16801 acaggctaaa agctatttgc tttcacagct tgaaaatgag cttgctcacg agaagtcggt
    16861 taagattatg gaatataccg aacagctcaa agaggaagca gacgacaagg caagaaacat
    16921 tatttcgctt gcaattcagc gacttgcagc tgagcaggtt tcagaggcaa caatttcggt
    16981 tgttcctctg cccaatgacg agatgaaggg cagaatcatc ggtagggaag gccgtaacat
    17041 cagagccatc gaaactctta cgggcgttga tcttattatt gatgatacgc cggaggctat
    17101 cacgctctca tgctttgagc ctgtaagaag agaaattgca agaattgcac ttgaaaagct
    17161 tattgcagac ggcagaatcc atcccgcaag aattgaggag atggttgaaa aggcaagaag
    17221 agaggttgaa tccgaaatca agcgtacagg tgagagagcc gtgcttgacg caggtgttca
    17281 taacattcat ccggagcttg tcaagcttct cggcagactt cgttaccgta caagctacgg
    17341 tcagaatgta cttgatcact ctcttgaggt ttcatacatt gcaggtatga tggcgagtga
    17401 gcttggtctt gatcctacaa ttgctaagcg tgcgggtctt ttgcacgata tcggtaaatc
    17461 cattgaccat gaggttgagg gaacacatat tcagattggt gtcgatcttg ccaaaaagta
    17521 taaggaaagc gacgctgtta ttcacgctat tcacgctcac cacggtgatg ttgaggcgag
    17581 aacaattgtt gcctgtcttg tacaggcggc agatgctatt tcagccgcaa gaccgggtgc
    17641 aagaagagaa aatgttgaaa actacattaa gcgactccag aagcttgaag aggttgcgtc
    17701 ttcttttgac ggtgttgagc gtacctttgc aattcaggca ggccgtgagg ttagagttat
    17761 ggttaagcct gaaattgtaa atgatgagcg tatgattccg cttgcaagag atatctgtaa
    17821 aaaaatcgaa gaagagcttg aatatcccgg acagattaag gttaatatta tccgtgaaag
    17881 cagagcgtcc gattttgcaa aataaaatat cttgatttta actacggttg acaggactat
    17941 gttgaccgta gtttttattt tgtagggacg atattatccg ccagtacggc gagccgccgt
    18001 acctgattcg tgtagatagg ttcattaaat gaaaaattga aaattgaaaa tggaaaattg
    18061 cggtcgggaa gatggtttgc aatataaaaa acatattatg cccgagcgta gtatagtagc
    18121 tgtttgcaca atttaatgta gcggcgaacg aggttcgccg ctacaaacgc tacaaagaat
    18181 atgctgtaat caaaaaaata atttgcaaga aaattaaata aacttgcaaa aattaaattt
    18241 gtgccgtagc ttagattttt gacctgttat tgtaaactgt aatttaaaaa acagcaaaaa
    18301 actttaaaaa atattcaaaa aagtattgac aatcggttaa taaggcttta taattcaaga
    18361 cagtaaggca aaggtcttac gaagttttcg gaaaactgaa tataaaaagg acaaggaagt
    18421 tcactttggt ttcttctgat tttgcaacaa atttttaaaa acttgcaaaa tcggataaaa
    18481 acattgtgag ctttattttt ttgcttcggc aaaaacggtt tatgtggagg ttaaaaatgg
    18541 attcaggaaa tataggcttt gtgctgcttt gtgcggcttt cgtatttgta atgactccgg
    18601 gtcttgcaat gttctacggc ggtcttgtac gaaagaaaaa tgttgtaaat acaatgatga
    18661 catcaatttt tattatcggt gtcggaattg ttatgtgggt gcttttcggt tattcgcttt
    18721 cgttcgcccc ttcgggaaat gcttttatcg gtgatttccg ttggctcggt cttgagggtg
    18781 tttcactcac ggagggctac acggatgatg cgtcaattcc gaatatggta ttctcagcgt
    18841 tccagatgat gtttgccgta atcactcctg ctcttattac gggtgcggtt gcaggcagaa
    18901 tgaagtttaa gtcaattttc gttttcacaa ttttctggtc gcttatcgtt tattatccgc
    18961 tcgctcacat ggtgtgggga cagggaggac ttttaggtgc agacggtatc ggtgctctcg
    19021 actttgcagg cggcgatgtt gtacatatca gttcgggtgt ttcggcactt gtactttgca
    19081 tactcctcgg caagcgtcac gattatgaac attcaattta cagaattcac aatatcccga
    19141 cagttgtact cggtgcgtcg ctccttatgt tcggttggtt cggctttaat gcgggttctg
    19201 cacttgccgc cgacggtctt gcggctcacg catttatgac aacaggtgtt tcagccgcag
    19261 ccgcaatgct ctcgtggatg ctctgcgatg ttatcaagaa caaaaagcct acgcttatcg
    19321 gtgcaagcac gggtatggtt gcaggtcttg taggtatcac gcccggtgcc ggctttgttc
    19381 cgatgtgggc ggcagttatt atcggtttga caacaagccc cgtttgctat attatgattt
    19441 catacggcaa gaaaaaattc ggctttgacg atgcgcttga cgcattcagc tgtcacggta
    19501 caggcggtat ctggggcgga cttctcacag gtgtgttctc atgcacggca atcaacagca
    19561 gtgcaggtaa cggtcttgtg tacggtgagt ttgcacagtt cggtgctcag gcggcaggta
    19621 tcggcatcac aattgtaatt gcggttgtcg gaacactcat ctgctacgga atcacaagac
    19681 ttcttacagg caaaatcagg gttgacctca gagatgagct tatgggactt gatgtttcac
    19741 agcacggtga atctgcttat ccgtcattta acggtcttga caactaacgg aggttttaaa
    19801 gtatggaaaa caataacgat attcagctta taaaaataga ggccgttgtt cgtgaagaaa
    19861 tgtttgtaga tgtcaaaaag gctctcacgg atatcggcgt aaacggaatt acagcgtatc
    19921 aggttgtcgg ctgcggaatt cagcgtggta tgtcggagta tgtaagagga cagaaggttg
    19981 atgttcaggt tttgccgaaa atcaagtttg aaattgttgt ttcttccgaa gaatgggaga
    20041 gaaaaacaat tgaggcaatc agaaaaacag cctttacggg caacccgggt gacggtaaaa
    20101 tatttaccta ctatcttaaa caggcggtta aaatccgtac gggagaaaca ggctacgatg
    20161 caattcagac tcccaacttt gacgactgat attttcctat tttcctatat caaaaaagtg
    20221 cgtaccgaat ttcggtacgc actttttgct tgtaaaattg tttatcttct tattgtaata
    20281 tcttccgggc tgacagtatt gtcacgaagt ctgttgtaat aatcgggatt tgaatgcttt
    20341 atgcttatcg gctttagctc gggagttgca aaacagtgtg ttgttctgcc tgttgcagaa
    20401 agttctccgt ttttattgtt gaagatttta tagtcaaaaa tcattcttga acccgtaaaa
    20461 aatacaaggc ttacttcaac ggaaaactca tcgccgaatt taagcggctt tttgtaacgg
    20521 gcatatgcgt caacatttgg aatatacaat ccttttgact gcatttcggc aacatcacaa
    20581 ccgatgtcgt gcatatactg aattcttgca tcttcaaaat atctgatata atttgagtga
    20641 tgaacaatgt tcatacagtc tgtttcatag tatgaaacct ttgctttgta taatttctcg
    20701 gtcatatttt ctccattaca tatatacgga aaatccgtaa ttatttttta agcaacaaac
    20761 cgtttttaag gtcctctgct gcctgctgtc ttattttgaa gtgctgcttt ttacctgtcg
    20821 ccgtgtgtgg caattctgtc acaaagcggt aatatctcgg agcctgatat tttgaaatat
    20881 tcggtgagct gtcgcagaaa ttgataagct ctgcaaccgt aaggctttcg tcggccttga
    20941 ttacatatgc cgcaacgcac tcgcctcttg ttgaatcggg aacacctgtt acgatacatt
    21001 cctgaacctt cgggtgtgag ttaagaattt cctcaattct tgcaggataa atattttcgc
    21061 ccttgctgat aatcatatca tcttttctgc ctgcaactgt aacaaactgc tcgctgttcc
    21121 aagtgccgat gtcgcctgtg tacatccaac ccttgtagaa cttgtctttt gtagcctttt
    21181 cgttatttac atagcagtag gtagttttgt tcggacattt gatgatgatt tctccctctg
    21241 ttttgttatc gcaggggact gtgtcatcgg gctctgcttt tctgtcgtca taaacattta
    21301 caagtcttac ctcatcatct gtgcatgatc tgcctgccga acctgccatt tcaggaaggt
    21361 catacggacg aagaaatgta ttccagaaag attccgttgt gccgtagccg ttaaaaatgt
    21421 tgggggagag aagttcctga tagcgaatgc aagcggcttt ttcaagcgga ctgcccatgg
    21481 taataatgcc cttgagcgaa gaaaggtttt tctgatgttt ttcctgacgg tttgcaagca
    21541 tctcaagagt tgacggaaca cctgtgatga atgtaatgcc gtatttttcg ataaagtcca
    21601 tgcagcttct cgggttgaat gttctcataa tcacaagaca cgcacctgca taaagggtag
    21661 gtgtaggacc gcccgaatga ataccgccac ggtgaaacca cggcgtcata ttcatcgtaa
    21721 tgtcaacggg tgagagcgga aagtgcataa ttacatcgtg agccgaaaga acttcgttga
    21781 ttgcgttaag cggaacgccc tttggagtgc ctgttgtacc tgaggtctga agtcttaaaa
    21841 cctcgtcata aatatgcggt gcatagtcaa caggcggatt ttcgtaactg ctgtccttga
    21901 tgtaatcgtc aaagaaaatg tgtccttcgg gaagagttac ttcctttttg gaattattaa
    21961 cgcagagaat caattttggc ttatgctcgc agatttcaag agcctgtacg gctgtttgca
    22021 taatgtctgc atcataaaca taaacaacag gcttgttgtg tcccataatt tctgcggttt
    22081 cgcccggtga taggttaaag ttagcgggat ttgagattgc accgagtttc tgcggcgcaa
    22141 tgtaaccgaa caaaaactgc ggtgagttgt aaagctgcat aaacacgatg tcgtttttct
    22201 tgacgccgtc ttttttcata gcgtttgcaa atctgtttgc atcggcatta agttctttgt
    22261 agctccatgt tctgccactc tgaggatcaa ttgcggcagg cttatccgaa aagcgtctta
    22321 catttctcat aaagccgtta agccatgtaa attcactttc aaatgtttct ctgaacatct
    22381 gtgaatcata tgttaaattc ataatatcct cctgtcctat tattatgtat gggtgtacat
    22441 tattttatac acacatatgt gtgttgcttg tatgttgttt tgtcggcaga ttatttgccg
    22501 tatttgccta cgataatact tgagttctga ccgccgaatc cgaatgcgtt tgacatggca
    22561 aagttgattt cggctttctt tgcggtattt gcaacaaagt caatgccggc acattcggga
    22621 tcaacctcgt tgagattgat tgtaggcgga actgtgcctg tttcaatagc cttgacacag
    22681 gtaattgttt ctgtaatacc gcctgcgccc atcatatgac ctgttgcgcc ctttgttgag
    22741 cttacatagg ggagagtgtt gccgaaaacc ttcttgattg aagatgtttc aactgtgtca
    22801 cccttgtttg ttgcagtacc gtgagcattg atgtagccga tgtcggaagg attgattcct
    22861 gcctctgcaa ttgcctgttt catacaagcg attgcacctc gtccttcggg atgcggagca
    22921 gttacatggt aagcgtcatc tgtgttgccg caacctctga tttctgcata aatctttgca
    22981 cctcttgcaa gagcgtgttc ctctgtttca agaacgagcg caccgccgcc ttcacctatt
    23041 acaaaaccgt cacgggtaac atcaaacgga cggcttgcag tgcttggcga atcgtttctt
    23101 cttgaaagag cttttgcgtt ggcaagcgaa taaattacaa gaggacagag aacggactct
    23161 gctcctactg cgagcataat atcagccttg ccgctctgca tacacataca agctgtgttg
    23221 attgcgtcac cgcctgacga acaagctgtg cttaccgtaa agctcggtcc ctgaatattg
    23281 tagtcgattg cgatatttgc ggcggcaatg tttccgagaa ttttgggaat aaaacgagga
    23341 cctacctttt tgtgtgatgc gcctgtgagt gcctcctgag tatatgcgat tgttgcaata
    23401 cctgccattg cagtacccat aacaataccc gttctttcgg gttcaatctt gattccgctc
    23461 tgtttcatgg cttcctcagc cgcaatataa gcgtattgca taaacggatc tgtttttctg
    23521 attaaatcct ttggaagata gtcggacgga ttaaagtcct ttacttcgcc ggcaatctga
    23581 acagcgagtt cagacggatc aaaagtctta attgtgtcaa tgcccgatgc acctgccgtg
    23641 atattattcc agtagttttc aacaccgatg ccgacaggtg ttaccgcacc cataccggtt
    23701 acaactattt tcttcattcg ttaagctcct tgttttaaaa taagttattt acattttgca
    23761 tattattatg ctatacttga attgtaccaa atatggaaaa attattcaat accgttgatt
    23821 atgcaaaaat gacgatttgg cagtcttaaa tttacagttt tgcataatat tgaaaaaaac
    23881 cgacaaaata ctgcgggaaa ttgtcaaaac ggaggttatt atggatataa atcagctcaa
    23941 ttgctttata actgtggctc agacgctcaa cttttcagag gcggcaaggc ggaactatgt
    24001 ttctcagtca accgtcagca gatatgtaag cgaccttgaa aaggagtttg gggttcagct
    24061 ttttacacgc tctcaccgtg atgttatcat aacaagcgag ggcaaggcac ttttgcccta
    24121 tgcgattgaa attgttgaaa ctttaaagaa agcaaaaacc gttatcaagc agatgcatga
    24181 cggaggtcag ggtaaaatta cgctcggctg tgatgttacc tcgctttcgt tcccgacaaa
    24241 atgtatagcc gactttgcgg aaaaatatcc gggaatcaca attgatgtgc gacagcttga
    24301 cgaggctgac cgcacgcttg caatcacagg cggtgaatac gacttctgct ttatgccgag
    24361 agatatggtt cccgaaagct caggtattga atcaatcatt acacataatg aaaccttgtc
    24421 cgttgtaaca gcgaaaaatt caaaattcag cggaagaaaa agcgttaagc tttccgaatt
    24481 ggctgacgaa agtattctgg tactttcgga atctgttgct ccgattgtgt atatggaaat
    24541 tatggatttg ttccgtacat ttcacataac cccgaaaatt gaaaattcat ttgacgatct
    24601 tgtttccata tatgtgtcat cgtcatcggg aatcggcgtg tctgtcatac caacctcggt
    24661 cgccgcatat acttctgacg aatacaccga ttcatacctt attgatgatg cggacacaag
    24721 catagcctat gttatggcat gggaaaagaa tatttcaaat cctgccgcaa agctttttat
    24781 ggaggctgtt aagaaatatg ccaagggaga tgacaacatt tacgggctgt aactgagcat
    24841 atttctcaat ataaattcca ataaagaact atagtgaaaa tttcacaaaa taaacacaca
    24901 ttattttcaa aaaaggtatt tactttataa atcttcggtg ttataattac ggtagtcaag
    24961 gagaagttga caaaatatcc tttcaggagg taattaaaat gtacagattt actttaccga
    25021 gagatctcta tcatggtaag gatgctctct caagtcttaa aaatttatcc ggcaaaaagg
    25081 ctgttgtagt tgtcggcggc ggttcaatga agcgtttcgg ttttcttgac aaggctgttg
    25141 actatcttaa agaagcaggt atggaggtta agcttatcga gggcgtagag cctgacccat
    25201 ctgttgaaac agttatgaag ggcgccgctg ttatgcgtga atttgaaccc gactggattg
    25261 ttgcaatggg cggcggttct ccgattgatg ccgcaaaggc tatgtgggta ttctatgagt
    25321 atcccaacac aacctttgag gaaatcatca ctccgtttag tttcccgaca cttcgtacaa
    25381 aggctaaatt ctgcgctatc ccgtcaacat cgggtactgc aacagaagtt acggctttct
    25441 cagttatcac agactatgca aagggtatca aatatccgct tgctgacttt aatatcacac
    25501 ctgatgttgc tatcgttgat cctgcacttg ccgaaacaat gccggctaaa cttactgctc
    25561 acacaggtat ggacgctatg actcacgcaa ttgaggctta tgtttcaact ctcaactgtg
    25621 agtacacaga tccgcttgca ctccacgcaa ttgagcttat tcacgacaac ctcaagaagt
    25681 cttatgaggg cgacatggct tgccgtgata aaatgcacga tgcacagtgc cttgcaggta
    25741 tggcattctc aaatgctctg ctcggtatcg ttcactcaat ggctcacaag acaggtgcgg
    25801 ctttcacagg cggtcacatt attcacggct gtgcaaacgc tatgtatctt cctaaggtta
    25861 tcaagtataa ctcaaaagat cctgttgcag ctgagcgtta tgctaagatt gctaagttca
    25921 tcaacctcaa gggcgaaaca acagaagaac ttgttgacgc actcatcgct gaactccgtt
    25981 caatgaacga tcagctcaac attccgcagg gcattaagaa ctacggtccg ggcggcgtta
    26041 aggctgacga gagcattatc gacgaaaaag aattcctcga aaagctcccc gaagttgcca
    26101 agaacgcaat tgccgatgct tgcacaggtt ctaacccacg ccagccaaat caggaagaaa
    26161 tggaaaaact tctcaaggct tgctactacg atacagaaat cgacttctga ttcgtttgac
    26221 aaatctattt gtacttatca cagctctccg ttttcgggga gctgtttttt taataaaaaa
    26281 tccgcataaa aatgctctta aaaggcataa aaatcatatg gacaatattg ctttgttgtg
    26341 ctaaaattga cttgaaattg atgaatttgg tgacataaaa tgaaaattgc agtaataaac
    26401 ggtcagaacc ataaaggctc aacctatcat attggcaagc aggttgccga taaattaggc
    26461 ggcgaggtta cggagttttt tcttccgaga gatttcggcg atttttgcgt aggttgtacg
    26521 cagtgttttc taaaatccga aacactttgt ccgcattatg agcgactgaa aaagattacc
    26581 gaggctatgg acgaggctga tgtactcatc ttcacaacgc ccgtgtatgt ttatcattgc
    26641 acaggctcaa tgaaagcatt tcttgatcat tacggttatc gttggatggt tcaccgtccc
    26701 gaacagaaaa tgttcacaaa gcaggcggtt gtaatttcga cagccgcagg ttcgggtaca
    26761 aaatctgcca acaaggatat cgccgacagt atgttctttt ggggcgttcc aaaaacttat
    26821 cgttacggtg tcaatgttcg tgcaacaaat tattcaggcg tcaccgatga actcaaaaag
    26881 aaaattgaca aaaagactac ctcaattgcc aataaggtaa agaaaaataa cggcaaagtt
    26941 aaggcaggtt taaaaacaaa aggctttttc tttattatga gaatgattca gaaaagcggc
    27001 tggaatgaag ccgataaaaa ttactgggca gaacgaggtt ggaacggtaa aaacagaccg
    27061 tggaaaaata cataaggaga tgttttaaat ggcaaaaaat actgaaatca gcttgagaaa
    27121 caaggttatt tattcggttt atgtccgtaa tcacacaaat gacggcacat ttaatgccgt
    27181 tgcagaggat cttgacagaa taaagtcact cggtactgac atcatttggt ttatgccgat
    27241 tcatccgatt ggcgtcaaga ataaaaaagg ctctctcggc tgtccttatg caaacaagga
    27301 ctatcgcaca acaaatccgg aatacggcac aatggacgat ttcaagagcc ttgttgaaaa
    27361 aattcacgca aagggcatga agtgcatcat tgatgttgtc tataaccaca cttcgcccga
    27421 ctcaaatctt tcggttgaac atcccgaatt tttctacaga aagccggacg gcaattttgg
    27481 caacaaagtc ggtgattgga gcgacataat cgaccttgat tattctaaca aagaattatg
    27541 gcagtatcag attgattcac tcgttatgtg ggcgaagatt gttgacggct tccgttgtga
    27601 tgttgcatcg ttcgttcccg ttgaattttg gaaacaggcg agagaggctg ttgcaaaggt
    27661 aaatcccgac tgcatttggc ttgccgaaag cgtacacagc tctttcaatg tgttctctcg
    27721 caagagcgga atttataccg caagcgacta tgagcttttt gacgcttttg atatggaata
    27781 tgactacgat atccgtgagg ttttcgacaa atatttgaag ggcgaaacat ctctttcaaa
    27841 ttatatggat atgttcaatt atcaggaggc aatttatccg cagaattacg ataaaatgcg
    27901 ttgccttgaa aaccacgatc agccccgaat ctgccattat gtaaagaaca ggagcgacct
    27961 tgaaaattac accgcttttc tgtatttctt gaagggttca accctcatct atgccggtca
    28021 ggagttcggt tgtgacgaaa ctccaagcct ttttgacaag gatgttttcc cacgcaacac
    28081 aggcattgat ttaagcaacc tttttgcaaa gcttgataca atcaagaaaa ccgttcttga
    28141 tgatgacgat tatttcaagg ctgatgcaga cgatgaaaac gatattgcaa ttcttgagcg
    28201 tgacaacaac aagagcaaaa aagtcggcat tttcagcctt aaatcaaagt cggctgatgt
    28261 aaaagttgac ctccccgacg gcgactataa aaacgaaatc agcggtgaaa cagttacagt
    28321 ttcaaacgga aaggttcatt gcaacggcaa agcaataatt atccgcaagt aaaataaaat
    28381 tccatataag aataatacaa cctccctgca aagcatttca gcttacgggg aggttgttct
    28441 ttatataaaa atccgtgttg catggttaca acacggattt cttggtttta tttaattgaa
    28501 ccgtcgccac ccataagagc ggtcattttt tcaatgcgtt ccaaaggaaa cggtggttcg
    28561 caaagcagtt ttttatatgc tcacttagct ttctttttat tattgccgaa aattttagtc
    28621 ggaataatgc ttccgaggaa tgcggcgagc aaaatccacg gaataactct tgcgtcaaag
    28681 cagagcaaaa gaagcattgt tacggcaagc ggttttttca ttgtgacacc gagaagtcct
    28741 gccgttacga ctgccgcaca gaatgccgtg tcaagtccgc cgagcattgc aacaccgtag
    28801 ccgatgctca ctccgcagaa gatgacaggg aagaagtgac cgcctttcca acccgacttt
    28861 atgcagatgt ttgtgagaac aagtttaagc acaccgctga taatgagaat ccacggtgca
    28921 aattcggcaa agctttcctt gacaacggta atactttctt cgcccgaaaa cattgtgagg
    28981 gggaggtatg tacccataac accgaggata attccgccga gagttgtgct gataataatg
    29041 ccgagtttac cctgaatgag tgcgaaaaat tttccgaaaa tctttgaaga tatgataaac
    29101 agaaaaccgc atacagcacc caaaagtgcg agaggaattc cccacaaacg ctcaaaattt
    29161 gtaatgttat aacccccgag tcgtggaagt cccatacttc cgccgaaaat cgagttaagc
    29221 aaaaacagag ttcccgccgc aaaaagtacg gcaatgatgt ttgaaataag ctttgttgaa
    29281 cggatatcgg taggcttttc ttcgctgtca agcctttctt cattgaccga tgcaagaccg
    29341 aaaagcggtg cgtagaatac cgcactcagg gttgcgctta tgccgatttg cattagttcg
    29401 ggaattttac ttttagcgtc tttcatatga cttcctgccc agtagcaaag tcccacgata
    29461 acgcctgtca aacctgcctc ggggccaaga cttccgccga aaacaagggg caaaagtgcc
    29521 gaaatgcaga gcaaaagcac cttgttgtac gggtaaaatt tatctttttt tacctttttc
    29581 ataacctcgt caagttcatc gggaacggca tttgtcagct tttgccatat tccgagaata
    29641 attccgcctg ccgtacagac gataatcgga taaaatttga aatcaaaatt tgacggaatt
    29701 gtatcccaca caagaccgat tcccaaaccc ataagtttta gaaacagcca tactatcagc
    29761 gaagtaaccg caccgctgaa tccgcaaaaa atgtagtata aaaaattgtt ggcaacagtc
    29821 cgtttagtca ttgtcgcacc tccgtgtgtt tataattgga ttataacata aaacacagca
    29881 aaaaaacaat tattccgcac aaaaagtacg aaataattgc tttttattat ttttttaatt
    29941 ttagagcctc ttctaaacaa aaacagctga caccaatgcc gatgtcagcc ataaaaacct
    30001 atttgatttt aaatttcaaa agcggtgctt acctttttga attctctttt tctgatgatg
    30061 aagtaggcaa tcaaaacgga tgtgcctgtg ataagccatg aaacggggta aaccataagg
    30121 agcatatcat aggtgtggaa tgccttgaat accgtgaaga gccagatgat tctgaacaca
    30181 acagaaccga agattgtgag gattgcaggg aggagtgaat gtccgattcc tctgagcgca
    30241 ccgccgctga tttcgtatga acctgtcata agctcaagga gcataacaat cgccattctg
    30301 atgattgcgt acttgataac ggtggggtcg gttgtgaaga aggtgatgaa aaatccgctt
    30361 ccgagcatga atataacgct cacacagcct gtgaaaatac agctgaaaag catacaaagc
    30421 ctaaacacct ttttgcatct gtcaaattga agtgcgccgt agttctgact tgtgaatgta
    30481 acggctgtct ggctgaacgc acatactata tagtatgtaa tgtactcaaa gttgagttca
    30541 gccgatgagc ctgcaaccgc ctgtgaaccg aagccgttta tggctgtctg aatgaataca
    30601 tttgaaattg agaaaaccat accctgaata cctgcaggag caccgatttt caaaatcata
    30661 atgagatgtt ctttcttgaa tgaaagtttt tttggattga ctctgattga ctcgtcttcg
    30721 tttctgatga ggaacatcat aacaagtccg gcgcttatgc cgtcggcaat tactgttgca
    30781 atgccgactc ctgcaacgct catatcgcag acgattacaa agaagaggtt caaaagaaca
    30841 tttacaatac ctgaaattac aagacagtag agcggacggc ttgtgtcgcc gacacttctc
    30901 aaaattgccg aaccaaagtt gtagagcatt acaaacggca tgcccataaa gtaaattctc
    30961 aggtatgttg ccgcaaggtc aattacatca tccggtgtgt tcatcagcct taacagcggt
    31021 gctgcaataa cctgaccgag tatcagaaga agaaatccgc atataagtga aagtgttatt
    31081 accgtgtgaa cagcttccga aaccttttta cggtcatttt gaccgatata tcgtgcaatc
    31141 aaaacatttg cgccgaccga aagtcccgtg aataacagca caagcatatt gatgagcgga
    31201 ccgttgcttc cgactgcggc aagtgcctga ctgcttgaaa atctgccgac caccgcaaca
    31261 tcggttgagt tgaaaagctg ttgaagaata ctgcatcctg caagcggaag tgtgaaaaga
    31321 agaatttttt tcccgagcgg accttccagc atatttatct ggtgtgagcg ccgtcgcttt
    31381 tcttttacag tattctgacc tttgtttctt ctaaacagac ttgcaaacgc aagtgagagc
    31441 atttttttga ctttctccat taatttctaa cctcttcaat cacataatct ttgtaaacaa
    31501 taccattctt ttccgttttc cacaattatt gtaatcctgt tgactgaaat aaacaagcaa
    31561 aataccttat aaacataaag tttactttgc tcacaatttt agttacaata actacctcgt
    31621 caaaattctt ctttttatgc ctttataata tggtaaaaac tgcttttctg catattttca
    31681 gaccgatttt tctcaataag caaggctttg aatttgttcg caatttgtgc cttttcaaca
    31741 aaagaaaatt gctgtcttgt aagcattgac gaaaaatttt ttaacctaca aataaatgat
    31801 tttaaaattg ttgtatatgc cgattaaatt ataatgatac aattatgata caagcttaat
    31861 ataatatatg tttaaaggga taagcgaaaa ggagtgaatg ttatgaagaa aaagattgct
    31921 gtaattgtgg ttgcagttgt tttatgcgtt gccgccgcag tatttgccgt gccgaaaatc
    31981 agtttctatg cgtgtgagcc taccgtgtat tttgatgttg agtactgcga caaggttgac
    32041 gctaagatgt ctgccgaaga tgccgaaacc gtgaagaaaa tgtttgaggg caaatccgca
    32101 tattttgatt caccgtcttg cggtttttcc gaaaaggcat caataagaat aggctgtaat
    32161 acatatatgc ctgcgtgtga cggtgatgaa attgttaagc acggatttat gtattttagt
    32221 ctttcaaaaa gcgaaaataa cgaacttcgc aagattatga aaaagtacgg agcagatacg
    32281 agaaaagcta tctaaaaccg ttgacagacg catttccgtg tgttaaactt ttctcgaaaa
    32341 atatacggag gtgtttttat gaaaaactac aactgggcaa cactcggttg cggagttatc
    32401 gcaaatgagc ttgcagccgc actcaaaagc agaggacaga aactttattc cgttgcaaac
    32461 cgtacccatg aaaaggcggt tgcatttgcc gaaaagtacg gcattgaaaa ggtttacaac
    32521 gatattgacg agctgtttaa agacgaaaat gttgacataa tttacatttc cacaccgcac
    32581 aacacgcata taaaatatct tcgcaaggct cttgcggcag gaaagcatgt tttgtgcgaa
    32641 aagtcgataa cgcttaattc agatgaactt gacgaggcaa tctctcttgc agagaaaaac
    32701 catgttgtgc ttgcagaggc aatgacaatt ttccatatgc cgatttacaa ggcacttctt
    32761 gaaaaggtaa attcaggtga ccttggcgag cttaggctta ttcagatgaa tttcggcagt
    32821 tacaaagagt acaatatgaa caaccgattt ttcaaccgca atcttgcagg cggtgcaatg
    32881 cttgacatcg gcgtgtatgc gctttcgttt gttcgtatgt ttatgagctc atgcccaaac
    32941 gatgtgttgt cgcaggttaa atatgcagaa acaggcgttg acgaacaagc gagtattctt
    33001 ttaaagaata aagaggagga aatggcaact gtcattcttt cacttcacgc aaaacagcca
    33061 aagcgtggca caatctcgtt tgacaagggt tatgttgaaa tctttgaata tcccagaggc
    33121 atggaggcaa agattacata cacggctgac ggccattgtg aaacaatctc ggcaggtcag
    33181 acaaaggacg ctttgcttta tgaggttgaa gatatggaaa aggcgataga gggcgacacg
    33241 gctcttatgc accttgacta cacaaaggat gttatgaaaa tcatgaccga catccgcaaa
    33301 agctggggca tgaaatatcc cgaagaagag taagtttttt gaatttaatt tgatactaaa
    33361 cacaaaactc caccgtgaaa tgcggtggag tttcctttat atatttgatt aaatcagaat
    33421 tagtagtttt ctgcgtg
//
LOCUS       DAOMVE010000003        30845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_204_length_30845_cov_3.884313, whole genome shotgun sequence.
ACCESSION   DAOMVE010000003 DAOMVE010000000
VERSION     DAOMVE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 30845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30845
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_204_length_30845_cov_3.884313"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    238..247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(321..1976)
                     /locus_tag="PLS64_00350"
     CDS             complement(321..1976)
                     /locus_tag="PLS64_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL42315.1"
                     /translation="MKKILSILLAGAMLAGCVLTGCSDSSSSKSDSSDGNTAKVADPI
                     EGVKATASTDKYRNYYEIFVNSFCDSNGDETGDLQGIISQLDYLNDGDPNSGDDLGVD
                     AIWLTPIMPSKSYHKYDVEDYYNIDPDFGTLDDFDKLIEECHKRGINVILDLVLNHAS
                     SKNPLFTKAVEEVADNKLDGNAEYFEIHKSSYFDSDTQTISLGNGYACEANFSQEMPE
                     WNLNSKKTREEFTKIAKFWLDRGVDGFRLDACKYFTNKETDGTEFLKWFYDTCKGIKE
                     DVYMVGENWTDDSDIQELYKSGIDSQFAFKFSTSTGTIISNIISQGGMATAKKIMNYD
                     NKMTESNPNAINAMFLSNHDQVRSGNALESQGLSSQKLAAAVYMLAPGNPFIYYGEEI
                     GVKAPNTTNDAAYRTQMVFDSDNLPDIYVNGIGDEPDVPTGGGVKQQLADKDSLLNYY
                     RRIITIKNQNPEIARGRIVGTQGFDDKAVGAYYVEYEDSKLLIIHNFSKNDAKELTIT
                     DDMIKNATLRADLIPESSSKHTELKDGKISVPPQSTVIIKSAE"
     gene            complement(2048..2911)
                     /locus_tag="PLS64_00355"
     CDS             complement(2048..2911)
                     /locus_tag="PLS64_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRL42316.1"
                     /translation="MIKSYKLRRHIANAFVYLFLIVMGFVWISPFIYLLMHSFRGGAE
                     IYGSTIIPQEWTLQNYIDLFTGSGGTASLNFPRWFLNTFVVACFSCVISTISVLMVSY
                     AFSRLRFKARKKFMNVGMILGMFPGFMTMIAIYYLLKAMGLTQTLVALVICYSCGAGL
                     GFQISKGFFDTIPRALDEAATIDGATKNQIFWKIILPMSKPIVVYTVLTSFIGPWTDF
                     ILAKIIMGDARDNYTVAIGLQLMIDQDFKNTYYKQFLAGSVVVAVPITLLFLKMQKYY
                     VEGVTAGGVKG"
     gene            complement(2908..4407)
                     /locus_tag="PLS64_00360"
     CDS             complement(2908..4407)
                     /locus_tag="PLS64_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRL42317.1"
                     /translation="MTYVEYKQLNPFQRFAYNTKKFFCNIPHAVAHFFTALGKAIVKF
                     FVGIGKGFANYGKTFVKGDWATKLSYLIFGVGDLSKGKYYKGILFFAVEVLYILYMAF
                     FGWGYLKMFPTLGIQAQRTEYINGIIPKQVPGDNSMLILLYSVLTLVITVVVFAIYIV
                     NIKDAYRHQIMKANGQKPTSFKYDMKQFLDGKYHITLMSFPVLMIGIFNVLPLIFMIL
                     IAFTNYDKQHMPPGTLFTWIGFDNFGSLFNLVEGAKKGYTFIKLTEWTLIWAVAATFS
                     NYILGLIFALMINKKGIKFKSLWRTLFVITIAVPQFVSLLLMNQMLQSNGAINILLSN
                     ITNSHVEIQWLNQDATLARWVVIIINCWVGIPYTILSMSGILMNIPEDLYESARIDGA
                     GPVKTFTAITLPYMLFVTTPQLITQFVGNINNFNVIYLLSNGNPTTEKFYQAGQTDIL
                     VTWLFKLTMNSQDYGLAAAIGILVFVICATLSLIVYNNSKSMKDEEAFS"
     gene            complement(4657..5994)
                     /locus_tag="PLS64_00365"
     CDS             complement(4657..5994)
                     /locus_tag="PLS64_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRL42318.1"
                     /translation="MKKILAILLAGMMTATALVGCGGGDASSKTDNGSKTDNGSSDGA
                     AKIEGSIVDDSYFGDEGTDSKPVTLKVWAPDAAVSLVKEQVEAFKKAYPNRKFKEISV
                     VAQGEKDAATNMLNDATTAADVFSFPSDQLNKLVDAGVISQVAFKDAVTEANSEETVK
                     AATLNDTLYAYPETNDNGYYLVYDKSVVSDEQAQTLEGILEACKKAGKKFIMNAGDGY
                     YACTFAFTAGVKIDGLEKDGITQKFVKYDEDEAVKTLQAFAKLIKDYRGTFQSLDVAN
                     IASGFASGKCGAGIDGSWDTASNQKALGDNFGAAKLPTINVNGTDKQLISMFGYKYIG
                     VNGSSKFPATAQILAHYLAGEECQLQRTEKLGWGPSNKKVQESKAVTDSPVLKAIGEQ
                     AKNACIQVNIANTFWDPMANLGNKIIADTCDPSDAAAMKKLLNETISNVRDEG"
     gene            6520..7986
                     /locus_tag="PLS64_00370"
     CDS             6520..7986
                     /locus_tag="PLS64_00370"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRL42319.1"
                     /translation="MKLKDLLINFEYTVQGNLDTEISDIIYDSRKVEKGTAFVCLKGY
                     TSDGHRYAKSAVEKGASALVISDSLDFEVPSDVAVVKVDDTRYALSLMSIEFFGHPED
                     ELYTVAITGTKGKTTTTAMVAEIFEHAGIKTGTIGTLGVVYGGETHKTDNTTPESYII
                     QKSMRDMINAGCKAMVIEASSIGLKHSRLAGMTFDVGIFTNFSDDHIGGVEHKDLAEY
                     LYCKSLLFRQCKFAAANIDDPAWKDITKDFKGSVLTYGFADNADLKGKNAHLLSENGF
                     VGVHFETEGIKNLSVDVDIPGKFNAYNALAAISAVSFFDEIEDQMIIDALKVAKVKGR
                     VERVPVPYNYTLIIDYAHNALSMENVLETLRQYNPHRLITMFGAGGNRPKVRRYEMGE
                     TSGRLSDLSVVTEDNSRFEDVMDIIEDIKTGLAKTDGKYVVVPNRRDAIKYCMDNAED
                     GDIIVLAGKGHEDYQEIKGVKYHMDEREIIADILNGKS"
     gene            complement(8064..8804)
                     /locus_tag="PLS64_00375"
     CDS             complement(8064..8804)
                     /locus_tag="PLS64_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL42320.1"
                     /translation="MMKKILVTGASGGIGSQTAVAFAKCGCDVALHYSKNSEKAESLA
                     EKLTNEYGINAFAVQADVSDYESVCKMFDEIDSRFGNLDVLVNNAGIAQQKLFTDITP
                     DEWKKMTGVNLDGVFYCSQQALKRYMIKNHSGVILNISSMWGQVGASCEVHYSASKAG
                     VIGLTKGLAKEVGLSGVRVNCICPGVIMTDMMKGFDEQTVQELKEETPLNMLGMPDDI
                     AETAVFLCSDRARFITGQIVGVNGGMII"
     gene            complement(8975..10666)
                     /locus_tag="PLS64_00380"
     CDS             complement(8975..10666)
                     /locus_tag="PLS64_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRL42321.1"
                     /translation="MADEIKNVEGEAAVQGNFIWDFIKEDTAEGGKFEGKKVHTRFPP
                     EPNGYLHIGHAKALCIDFGTAEHFGGICNLRMDDTNPTKEDVEYIDAIKEDIKWLGYD
                     WDDRFFYASQYFDQMYEFAVELIKKGLAYVCELTPDKVREYRGDTQTPAKSPYRDRPI
                     EESLDLFERMKNGEFEDGKMTLRAKIDLASGNFNMRDPVIYRINRLKHHRTGDKWCIY
                     PMYDFAHPIEDALEGITHSLCSLEFEAHRPLYDWVINNISVPCKPRQIEFARLGINNT
                     VMSKRKLRELVEKNYVSGWDDPRMPTLCGLRRRGYTPKSIRSFIEQIGVSKVNSIVEY
                     GFLEHCLRDDLNETAERTMCVLKPVKLVITNYPEGQSEEFEVENNPNRPEDGTRKVTF
                     SRELYIEETDFMEEPVKKYQRLYPGNEVRIKSAYIVKCTGCKKDENGNVVEVYAEYDP
                     ETKGGNTPDGRKVRGTIHWVDANNCLDAEVRLYDNLFTVPDPDTGDRNFLDYLNENSL
                     EVLTGCKAEKNIAGATAPKSYQFMRHGYFCIDNKDSSPEHLVFNRSVSLKDSFKK"
     gene            complement(10692..12149)
                     /gene="gltX"
                     /locus_tag="PLS64_00385"
     CDS             complement(10692..12149)
                     /gene="gltX"
                     /locus_tag="PLS64_00385"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRL42322.1"
                     /translation="MAEKTVRTRYAPSPTGFMHVGNLRTALYEYLVAKSQNGKFVLRI
                     EDTDRERLVEGAVDVIYDTMKLAGLKHDEGPDIGGDFGPYVQSERKDMYLPYAEQLIK
                     EGKAYRCFCTKERLEKLQEDSVGGGYDRHCRNLPQEEIDRLLAEGTPYVIRQKMPIEG
                     STTFTDAVFGEITVDNSELQDQILIKTDGYPTYNFANVIDDHTMGITHVVRGCEYLSS
                     TPKYNLLYEAFGWEIPTYIHLPLIMGKDADGNVSKLSKRHGATGFYDLINEGYLPQAI
                     INYIALLGWCPKDNQEIFTLAELEKEFDVSGISKSPSIFDYDKLSWFNGEYLKAMTPE
                     EFTDVCMPYYKKVFGDREMPFEKFAEILQKRTTKLTDIPEMLGFFAELPEYDKELFVN
                     KKSKTNLENAPVVLREAYERLKALPTWDNTSIHDCLINMAVEKELKNGTVMWPVRIAA
                     AGKTVTPGGAVEILDILGREESLKRLEIGLEKLGA"
     gene            complement(12436..13785)
                     /locus_tag="PLS64_00390"
     CDS             complement(12436..13785)
                     /locus_tag="PLS64_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HRL42323.1"
                     /translation="MMKKALSVALCILMAGTVLTACSNPDEFSDTENLSLKYTRSDKT
                     DDTFNYADGAKTAPSSYDTYSAKITDFELRLFRNRHKSGSYVFCPANAVLNLEAMANG
                     ASGDTQEEITNALCSGVTLENMNQCASYFASRIQSVASSDKSPESSQSSEKDSEKNSD
                     KSFVKLCNSLISNDNADIMTGFLQTTKDYYDTNVLRFNFSDSDALKKLDKKYADFTNN
                     GSVFENLDTKQSVISLSATDICDRWLNPYAQTDIEKAKFKSADGEKEVTYMTSNETYM
                     KTNKATAVMKYFSQTPLKMMVIMPNEGVSLDDYATDFTSLEYTTLLDSVDVTKTAKAK
                     IPEFSVSGDKSAENITQIVEKSGINSSFTADRSRYKNLSRSDDIAFSAMYDIAPSLSI
                     NAGGIGGTQSNGDKAELEKRTKELSKTDVTVEFNRPFMFVILDNESDIPLYMGTYAG"
     gene            complement(13782..14924)
                     /locus_tag="PLS64_00395"
     CDS             complement(13782..14924)
                     /locus_tag="PLS64_00395"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL42324.1"
                     /translation="MKIIIFTASTGGGHKRAAAAIEAKIKAVSPDTKVKVIDAMKTIG
                     RVYDKTVCDGYHFMATKIPKVYGKFYKITDRRTLMYKAVMQSNTMMSAKLLDTINEYK
                     PDAIIMCHPFVTTMISKLRRQHKIDVKAISLITDYDAHRTYIVPYVDAYVLAEPDMAT
                     KLIDEYGVDKSIIYPLGIPIFDRFTEPFDKKAICEREGLDPNKPTILLMAGSFGVTSV
                     LSFYKALVEQAPEMQFIVITGRNIKLFANLEKVIEETGMQDNTKLLYFVKNVEDYMHI
                     SDLIVTKPGGLTVTESLACSLPMAIYSAFPGQERDNAEFLLNKGAAIMLRKKTGADDI
                     VNLVKDKEKLDEMKEKCRELHRPDSAEKIFRLAQKLCNDSKGGNDK"
     gene            15375..17093
                     /locus_tag="PLS64_00400"
     CDS             15375..17093
                     /locus_tag="PLS64_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRL42325.1"
                     /translation="MNRNFMDDILVVDEDLVDINCDGNRQSDNKKGKHSSGGRMAKGE
                     KKSKKKLAIIISSVAAVAVALGVAGFCIFGGNLFNSVEEAIAGEFKFPDGTTVSGVSI
                     SGKTYDEAKKALEEKEESFIKPLDISVDVNGVISKVTEKDFKYTYDIESVLNEIKNEA
                     TDPSVETSTSKKSYTVTATVIAESVDEAAKKVAKKNYKEAENARVSKFHPYAKKRFEY
                     TEASQGQKVNETDLANQFKGVFASGASEYRIIADVEKTDAKISVDDLKKNIVLLSTYE
                     TVSTNTENGTENMRVSLKACNGSVIEPGATWSFNKCTGNSNLESLGYKPAGVISNGKS
                     DIGIGGGICQSSSTIYNAAVRANMKVEERYCHKWASSYVPTGLDATIDYGNLDLKLSN
                     PTDYQMFLECKVVDNTLYVSFWGWKSDSYDLIMTRNKLTNQGSSSYTVKAWRVYYKDG
                     KEIDSESLGSSTYDTENGYVFIDANNDPRAKYGDDVVIPDETVPKNDDSSSSSSSSQS
                     SYSEPSYSSSSSSSHSSNSSSSSSSSHSSSSHSSSSQSSSESKTEPQPKPDPEPTPTE
                     PESGEE"
     gene            17167..18177
                     /locus_tag="PLS64_00405"
     CDS             17167..18177
                     /locus_tag="PLS64_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HRL42326.1"
                     /translation="MIKRTGAYTIAFESQPVIAGWGSVVGKKEAEGPLKNYFDKIIYD
                     SYDGCDTFEQAESMFQGEALEKALERSKTHANEVDCVFAGDLLNQCIGSSFGLMKFGI
                     PYLGQYGACSTMAQSLIMAASAVESGGAKVSACVTSSHFASAERQYRFPLEYGGVRTP
                     TAQWTVTGAGSCILKSLKKGICVARATVGRIVDLGVSDANNMGAAMAPAAAQTLECYL
                     EDTKTSPEDYDMIITGDLGEVGSKSLYDLLERDGINIRKQHNDCGLMIFERSNQDVHA
                     GGSGCGCAASVLCSKIMDDFQNGLVQNILFMATGALMSPTSSGQGANIPSIAHLVNLK
                     IK"
     gene            18320..18868
                     /locus_tag="PLS64_00410"
     CDS             18320..18868
                     /locus_tag="PLS64_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL42327.1"
                     /translation="MFGRIQNIDNRVLDNISRIHKPALNKIMITASRAGNAGIVWWAI
                     CLPFLINSNWRATGANFVFGLAWAHLMGEIIIKHIVKRVRPCHTLDDDEQLIDRPRFY
                     SFPSGHTTASFAMVGVALMRCKVITFMPILMLAMLIAFSRIYLRVHYLTDVIAGMILG
                     LTCGICSVLIFNTVVPMWFPMV"
     gene            complement(18996..19337)
                     /locus_tag="PLS64_00415"
     CDS             complement(18996..19337)
                     /locus_tag="PLS64_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42328.1"
                     /translation="MKIAVIGSRTLTVRNLEKYIPKDTTEIISGGANGIDRCAKEFAV
                     KNNIRYTEFLPEYKIHGRYAPLKRNLEIIDNADFVLAFWDGKSRGTKFVIDNCRKINK
                     PIRVLISKGCL"
     gene            19447..19635
                     /locus_tag="PLS64_00420"
     CDS             19447..19635
                     /locus_tag="PLS64_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42329.1"
                     /translation="MKGKPKHLSLRIDEDLLRKFEYVAKYDDRSMNWYLLHLIKNEIA
                     DFEEKHGEIPTASDSKTE"
     gene            complement(20049..21305)
                     /gene="glyA"
                     /locus_tag="PLS64_00425"
     CDS             complement(20049..21305)
                     /gene="glyA"
                     /locus_tag="PLS64_00425"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRL42330.1"
                     /translation="MSEKTNIATLDYLNEYDPAIGNAMTDELKRQRRNLELIASENIV
                     SPAVMAAMGSLLTNKYAEGYPGKRYYGGCQCVDVVEEIARQRACELFGAEHANVQPHS
                     GAQANTAVYFAMLNPGDTVMGMNLNEGGHLTHGSPVNISGKYFNFVPYGVDPETHRID
                     YDKVLEIAKECKPKMIVAGASAYPRIIDFAKLREIADAVGAYLMVDMAHIAGLVAAGV
                     HPNPVPYCEFVTTTTHKTLRGPRGGLILCREEFAKQIDKAIFPGTQGGPLMHVIAAKA
                     VCFGEALKPEFKEYGKKIVSNCKALADGLLKRGNKLVSGGTDNHVLLMDLRDTDVTGK
                     ELEARLDDCYITVNKNTIPGEPRSPFVTSGVRIGTAAVTTRGLNEEDMDKIAEYITLV
                     LNDYENSKEKVRAGVEEICKKYPLYE"
     gene            complement(21364..22338)
                     /gene="dusB"
                     /locus_tag="PLS64_00430"
     CDS             complement(21364..22338)
                     /gene="dusB"
                     /locus_tag="PLS64_00430"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HRL42331.1"
                     /translation="MDNLNIGGVPLKSHAVLAPMAGVSDRAYRELCVRFGAAYCVSEM
                     VSSKALSFNSKKSEELMEISDLERPCGIQIFGDDPKCMADAAKHALENKPDIIDINMG
                     CPAPKISSNGSGSALMKNPRLCGEIVKAVTAVTDIPVTVKIRKGWDDDSVNAVEVAKI
                     CESAGAAAITVHGRTRQQYYKPPVDYDIIRAVRESVSVPVIANGDIDSAERAKEVMDI
                     TGCDLVMIGRATLGNPWIFSQINAYLENPNVKIHTPDLEERLGVMIEHIGKMVEYKGE
                     HMAMLQARKLVVGYFKGMKGAAALRNEAGKIKTLDDLYELRQKALSLQ"
     gene            complement(22360..22878)
                     /locus_tag="PLS64_00435"
     CDS             complement(22360..22878)
                     /locus_tag="PLS64_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42332.1"
                     /translation="MKSDFKKIFRKRILPTVLAVLALSVCSITAFAVGEDDSQTPDPQ
                     PDPVVTEYVPVETEPQYTEPIYTEPVYTEAPTTEYVEPETEEPTTEYVQPETEAQKQE
                     ETQYQNQYVETKATDSTEFQAPTIAKTVSEKKYSTSYTAGIVSWICVGLGVVAIAVVM
                     ISTKVGARKRIR"
     gene            complement(23054..23455)
                     /locus_tag="PLS64_00440"
     CDS             complement(23054..23455)
                     /locus_tag="PLS64_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRL42333.1"
                     /translation="MAYNDNSINREVILNYAFSTYGTEPEYPWESSPDNAVLRNMMSG
                     KWYAIIMCVKRQILGLDGDGFVDVMNVKCDPILVGSLRMKNGFLPAYHMNKDKWISIL
                     LDGSVSKEEIFPLIDESYNLVMPKAKKRSKN"
     gene            complement(23455..23631)
                     /locus_tag="PLS64_00445"
     CDS             complement(23455..23631)
                     /locus_tag="PLS64_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42334.1"
                     /translation="MNGNKPIEQIIENAAASVEMEGYTIDSKSKEWCQKLLRNEITMQ
                     EYISLVKKKAGVKA"
     gene            complement(23711..24787)
                     /locus_tag="PLS64_00450"
     CDS             complement(23711..24787)
                     /locus_tag="PLS64_00450"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRL42335.1"
                     /translation="MKRVGILTSGGDCQGLNATIRAVAKTLYNQLGNDVEIIGIIDGY
                     RGLIYGETRKMKPENFSGILTMGGTILGTSRQPFKLMRVVDENSVDKVEAMKKNYKKL
                     GLDCLVVLGGNGTQKTANLLREEGLNVVSLPKTIDNDLWGTDKTFGFQSAVDIATNVI
                     DCIHSTATSHGRVFIIEVMGHKVGWLTLYAGIAGGADIILIPEIPYDINSVVKTIKSR
                     TAGGKNFSILAVAEGAISKEIAALPKKQRKAAVAEMKYPSISYQIAHDIEEATGQETR
                     VTVPGHFQRGGSPDAYDRVISTRFGVKAAELIINKDYGKMVALVNNKVVAVPLEDIAG
                     KLKSVPKDSEVVETARKMGISFGE"
     gene            complement(24784..26964)
                     /locus_tag="PLS64_00455"
     CDS             complement(24784..26964)
                     /locus_tag="PLS64_00455"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HRL42336.1"
                     /translation="MSNSKNNNKKTSEKAKLMSKWQKMPYTNRELSWVDFNARVLEEA
                     FKKTNPIMERCNFLSITASNLDEFFMVRVAGVLDQIHHGRTSRDASGMTPTEVMDGLV
                     EKIHEFAKKQYSCYNRSIIPSLRSAGIVFKEADELDGDQKAFVEEYFEKVVFPVLTPM
                     AVDTSRPFPMLANKSLNIAVRLTNAENEEFFALVQVPSILPRFLELPTHEGRAFILLE
                     KIIAMHLGELFELYNIKQYDPFRITRDSDLDIDEDTEDLMAEIKKSIRKRKRGEPVRL
                     EFGKKCNREIREFLVDALDVTEREIYFQRGPLDLTFLSKFAHIKGCEDMCFEPIVPVN
                     PPAEFCGYDDIFEAIREKDRLVHHPYESFDVVVDFVKKAATDPNVLAIKQTLYRVSGN
                     SPIVAALIKAAENGKQVTVLVELKARFDEENNIQWATKLEKAGCHVIYGLTGLKTHCK
                     ICLVVRKDKDGIRRYLHMGTGNYNDSTARFYTDIGMFTCREEYGVDASSLFNVLTGYS
                     TPPEYNKFIVAPHGMRPFFEAMIIQETENAKLGLPAKITAKVNSLVDPEIISLLYDAS
                     NAGVKIDLIVRGICCLVPGVHGFSENIRVISIVGQLLEHSRIFIFENNSNPLYYMGSA
                     DWMPRNLDRRVELVFPVEDEDIKKRVQEVITVSFKDTVNARQQLSTGVYKSVDKRGKH
                     KTNCQKFFSKLALKAQKQAMKKTYASTVGTPIVPVEVKKEDSEQ"
     gene            27291..28646
                     /locus_tag="PLS64_00460"
     CDS             27291..28646
                     /locus_tag="PLS64_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRL42337.1"
                     /translation="MSKKRGSFSGGLGFVFAAAGSAVGLGNLWRFPYYAAKYGGGTFI
                     FIYIILALTFGFSLLTAEIAIGRKTKLSPILAYKKLCSKFSFLGYLATLVPIIIIPYY
                     CVIGGWVTKYMCVYAQGLVAPAAQDNFFSDFISDPLSPIVFFLIFMLLSAVVIMLGVN
                     KGIERLSKFLMPVLLVLTVGISIYTLTLPNAMEGVSYYLIPDFSKLSIWTFAAALGQL
                     FFSMSIAMGIMVTYGSYTKDEVNLTKSVNQIEIFDTAVALLAGLMVVPAVYVFSGEEG
                     LGASGPGLMFITLPKVFNEMPAGVIIGFLFFILVFFAAITSSISVMEAIVSSLMDKFK
                     LTRIKSCLIVIGICLIMGIPSSLGNGIWSNVKILGMDFLTFFDFLSNSIIMPIVALCT
                     CILIGWVIKPKTIADEVTRNGERFSRRALFDVMIKYIAPICLVFILISYTLAQFGIIS
                     L"
     gene            28828..30774
                     /gene="metG"
                     /locus_tag="PLS64_00465"
     CDS             28828..30774
                     /gene="metG"
                     /locus_tag="PLS64_00465"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRL42338.1"
                     /translation="MCQKKPFYITTPIYYPSGNPHIGHCYTTVACDSIARYRRMQGYD
                     VMFLTGTDEHGLKIEQKAAEKGVTPKEYVDEIVKTFKGLWKFMNISYDRYIRTTDDYH
                     IETVQKIFKELYDKGYIYKGEYKGKYCTPCESFWTESQLIDGKCPECGREVTEAKEEA
                     YFFKMSSFADRIEKLLTETDYLQPKTRAVELVNNFIKPGLEDLCVSRTTFKWGIPVTF
                     DEKHIVYVWIDALSNYISALGYKNEKFDEFDKYWPADVHMVAKDIMRFHAIIWPAMLM
                     ALDLPLPKHLAVHGWITFNGQKMSKSLGNVVDPFVLGERYGADAIRYHIMREMALGAD
                     SSFSNEIMINRINSDLANGLGNLVSRTVAMVQKYFGGTLPTERESGEFDDDLIETATS
                     LRAKVDDFMDKTQLQNALAEIFKLVSRANKYIDETAPWVIAKDETKKARLATVLYNLL
                     EAIRIACTLLSAFMPTTMPKALEQIGACEKCAGYENCDKFGVLPADVTVHKGDALFPR
                     IDVEKEIEELNSIIKNNEGESEETKALREELEGVAQIGIDDFAKVDLRVCEVKTCEPV
                     KKSKKLLQFTIFDGVKDRTVVSGIANYYKPEELIGKKIILVQNLKPAKLCGVESCGMI
                     LSAVHGDDLKVVFVDNMPVGSKIC"
BASE COUNT         8628 a         6738 c         6344 g         9125 t
ORIGIN      
        1 ttgtttttaa atataatttc tgccgttact cacgggagaa cgaggttttc ccctacaaaa
       61 cagcagtcat tttgcgacat ttataaaatt acatctgtca aacattttgt gatattactt
      121 gcagggctgt gaaataacag ccttacaaaa acaagaaggc acataaccgt cattgttttc
      181 ggttatgtgc cttttgtggt ttatatatcc ttatacaaat gttgcaaagc ttctgtgnnn
      241 nnnnnnntca actttggcac attgttgtgc tcggatgata tagtttttaa tacaaaataa
      301 ctgtctgcac gaacaaaaaa tcattcagca gacttaatga ttaccgttga ctgtggcggt
      361 acagagattt ttccgtcttt aagctcggta tgctttgaac tgctctcggg gataaggtct
      421 gcacggagag ttgcattctt aatcatatcg tctgtaattg taagctcctt ggcgtcattc
      481 ttgctgaagt tgtgaataat gaggagcttt gaatcctcat actcaacata gtaagcgccg
      541 actgccttat catcaaagcc ctgtgtgcct acaattctgc cccttgcaat ttcggggttc
      601 tgatttttga ttgtgataat tctacgatag tagttgagga gagaatcctt gtcggcaagc
      661 tgctgtttta caccgccgcc ggtaggcaca tctggctcat ctccgatacc gtttacataa
      721 atatcgggaa ggttgtcgct gtcaaaaacc atctgtgttc tgtatgccgc atcgtttgtg
      781 gtattcggag ccttgacgcc gatttcctct ccgtaataga tgaacggatt acccggcgca
      841 agcatataaa ctgcggctgc aagtttctga gaagaaagtc cctgagattc aagtgcgttt
      901 ccgctacgaa cctgatcgtg gtttgaaagg aacattgcat tgatagcatt gggatttgac
      961 tctgtcattt tgttatcgta gttcataatt tttttggcag tagccatacc gccctgcgaa
     1021 atgatgttgc tgataattgt gcctgttgag gttgaaaact taaatgcaaa ctggctgtcg
     1081 atgccggatt tgtaaagctc ctgaatatcg gaatcatcgg tccagttctc gccaaccata
     1141 tagacatctt ccttaatgcc cttgcaggtg tcgtagaacc acttgagaaa ttctgttccg
     1201 tcggtttcct tatttgtaaa gtatttgcaa gcgtcaagtc tgaagccgtc aactcctcgg
     1261 tcaagccaga atttcgcaat ttttgtaaat tcttctctcg tctttttaga attgagattc
     1321 cattcgggca tctcctgaga aaagttcgcc tcacaggcat aaccgtttcc aagactgata
     1381 gtctgtgtat cgctgtcaaa atatgaggac ttgtgaattt caaaatattc ggcattgccg
     1441 tcaagcttat tatcggcaac ctcttcaaca gcctttgtaa agagcggatt ttttgaagat
     1501 gcgtgattca aaacaaggtc aagaattaca tttatgccac gcttgtgaca ttcttcaata
     1561 agcttgtcaa aatcgtcgag cgtaccgaag tcgggatcaa tattgtaata atcttcaaca
     1621 tcatacttat gatatgactt tgacggcata atcggagtaa gccagattgc gtcaacaccg
     1681 aggtcgtcac ctgaattcgg atcgccgtca ttaaggtagt caagctgtga gatgattccc
     1741 tgaaggtcgc ctgtttcgtc accgtttgaa tcgcagaaag agtttacgaa aatttcgtag
     1801 tagtttctgt atttatcagt tgatgctgtt gccttaacgc cttcaatcgg gtcggcaact
     1861 tttgcagtat ttccgtcgga tgaatctgat tttgatgatg aagaatcaga gcagcctgta
     1921 agcacacagc ccgcaagcat tgcaccggca agaagaatgg aaagaatttt tttcatagta
     1981 tcctccgtaa aattaaaatg ggcggcagcc tgtgactgcc gcccgtgaga gcttgtatat
     2041 attgggatta accctttacg ccaccggctg ttacaccttc aacataatac ttctgcatct
     2101 tgaggaagag aagtgtaatc ggaacagcaa caacaacaga acctgcaagg aactgcttgt
     2161 agtatgtgtt cttaaagtcc tgatcaatca ttagctgaag accgatagca acagtatagt
     2221 tgtcacgagc atcacccatg ataatcttag caagaatgaa gtctgtccac ggtccgataa
     2281 atgaagtaag aaccgtgtaa acaacaatcg gctttgacat aggaagaata atcttccaga
     2341 aaatctggtt cttggtagca ccgtcaatgg ttgccgcctc gtcaagtgct cttggaattg
     2401 tatcgaagaa gccctttgaa atctggaaac caagaccggc accgcagctg tagcagatta
     2461 caagagcaac gagagtctga gtaagaccca ttgccttgag aaggtagtaa atagcaatca
     2521 ttgtcatgaa gccggggaac ataccgagaa tcataccaac attcataaac tttttacgag
     2581 ccttaaatct gagtcggctg aatgcgtaac taaccataag aaccgaaatt gtagaaatta
     2641 cgcagctaaa acaagcaact acgaatgtgt taaggaacca tctcgggaag ttaaggcttg
     2701 cagtaccgcc tgagcctgtg aacaaatcaa tgtagttctg gagtgtccat tcctgcggga
     2761 taattgtact tccgtaaatt tcagcaccgc cacggaatga atgcattaaa agatagatga
     2821 acggagaaat ccaaacgaaa cccattacaa taaggaacag gtaaacaaaa gcgttcgcaa
     2881 tgtgtcttct taacttgtaa ctttttatca tgagaacgcc tcctcatcct tcattgattt
     2941 tgagttatta tacacaatga gtgagagcgt tgcgcaaata acaaatacca aaatacctat
     3001 cgcggcggca agtccgtagt cctgagagtt catggtcagc ttaaagagcc atgtaacaag
     3061 gatatcggtt tgaccggcct gataaaattt ttcggtcgtc ggattaccgt ttgacaacag
     3121 gtaaataaca ttaaagttat tgatgttgcc gacaaactgg gtaatgagct gaggagttgt
     3181 aacaaagagc atatacggaa gtgtgatagc cgtaaatgtc ttgacaggac ctgcaccgtc
     3241 aattcttgca gattcgtaga gatcctcagg aatattcata agaatacccg acatagagag
     3301 gatggtatac ggaataccta cccaacagtt aatgatgata actacccaac gagccagcgt
     3361 cgcgtcctga ttcagccact gaatttcaac atgactgttt gtaatgtttg aaagcaggat
     3421 gtttatagca ccgtttgact ggagcatctg gttcatgaga agaagtgata cgaactgagg
     3481 aacagcaatt gtgataacga aaagtgttct ccagagtgat ttaaacttga tacccttctt
     3541 gttgatcata agagcgaaga tcaaaccaag gatatagttt gagaatgtag ctgcaacagc
     3601 ccagataaga gtccattctg tgagcttaat aaatgtatag cccttcttag caccttcaac
     3661 gaggttgaag agtgagccga agttgtcaaa accaatccat gtgaacagtg ttcccggagg
     3721 catatgctgt ttatcatagt tggtaaatgc aatcaaaatc ataaatatga gcggcagaac
     3781 attgaagata ccgatcataa gtacagggaa tgacataagt gtgatatgat acttaccgtc
     3841 aaggaactgc ttcatatcat atttgaatga agtcggtttc tgaccgttag ccttcataat
     3901 ctgatgtctg tacgcatctt tgatgtttac tatgtaaata gcgaaaacca ctactgtgat
     3961 tacgagtgtc aataccgagt aaagcagaat aagcattgag ttatcgccgg gtacctgctt
     4021 tggaatgata ccgttgatat attcagttct ctgagcctga ataccgagtg tcgggaacat
     4081 tttgagatat ccccaaccga aaaaagccat ataaaggata tagagaactt caacagcgaa
     4141 gaaaagaata cccttgtaat atttaccttt gctaagatca cctacaccga aaataaggta
     4201 ggaaagtttt gtagcccagt cgccttttac gaaggtcttg ccgtagttgg caaagccctt
     4261 gccgatgcct acgaagaact taactattgc cttgccgagt gctgtaaaaa aatgggcaac
     4321 ggcatgagga atgttacaga aaaacttctt tgtgttgtag gcaaatctct ggaaaggatt
     4381 gagctgtttg tattcaacat atgtcattag gcgctctcct taataatttg caaatctctc
     4441 taatacacca aactaataat gtcttttacg ctatcttata atagttgccg ttcgtaataa
     4501 aacattatta gtcaggcgaa tacacaaatt gataacgcaa gcggacagtc cgcatttttc
     4561 tttttttacg ggacactccg ttttcggagt gtcccgcagc taattaatcg attaaattaa
     4621 atcaatttaa cttacttaat ttaacttact taacgattag ccctcatccc taacgttaga
     4681 aattgtttcg ttaagaagct tcttcatagc agctgcatct gaaggatcgc atgtgtcagc
     4741 gatgatcttg ttgccaaggt tagccatagg atcccagaat gtattagcaa tattaacctg
     4801 tatacaagca ttcttagcct gctcaccgat agccttgaga acaggagaat ctgttacagc
     4861 tttgctttcc tgaaccttct tgtttgaagg tccccaaccg agcttctcag ttctctggag
     4921 ctggcactct tcacctgcaa gatagtgagc aagaatctga gctgttgccg ggaacttaga
     4981 agaaccgttt acaccgatgt acttataacc gaacattgag ataagctgct tgtctgtgcc
     5041 gttgacattg attgtaggaa gctttgcagc accgaagttg tcaccgagag ccttctggtt
     5101 agaagctgta tcccatgaac cgtcgatacc tgcgccacac ttaccgcttg caaaacctga
     5161 agcgatgtta gcaacgtcaa gcgactggaa tgtacctctg taatccttga tgagctttgc
     5221 aaaagcctga agtgttttaa cagcttcatc ttcgtcatat ttaacaaact tctgtgtaat
     5281 accgtctttt tcaagaccgt caattttaac acctgcagtg aaagcgaaag tacaagcata
     5341 gtaaccgtca ccggcattca taatgaactt cttgcctgcc ttcttacaag cctcaagaat
     5401 accctcaagt gtctgagcct gctcgtcgga aactactgac ttatcataaa cgagatagta
     5461 accgttatcg tttgtttcag gataagcata gagagtatca ttaagtgtag ctgcttttac
     5521 agtttcctca gaattagcct ctgttacggc atccttaaat gcaacctgag aaataacgcc
     5581 tgcgtcaacg agcttgttga gctggtcact cgggaagctg aatacatctg cagctgttgt
     5641 agcatcgtta agcatatttg tagcagcatc cttttcgccc tgtgctacaa ctgagatttc
     5701 cttaaatttt ctgttaggat aagccttttt gaaagcctca acctgctctt taacaaggct
     5761 tacagcagca tcaggagccc ataccttaag agttacaggc ttgctgtctg taccttcatc
     5821 gccgaagtag ctgtcgtcaa cgattgagcc ttcaatctta gcagcaccgt ccgaagaacc
     5881 gttgtctgtt tttgaaccgt tgtctgtttt cgatgatgcg tctccgccgc cgcagccaac
     5941 aagtgcagta gcagtcatca tacctgccaa taaaatggcg agaatctttt tcattggaaa
     6001 aatccctctt tctgaatatt ttcgtagtgt cctttcggat tacatacttt ctttgaggat
     6061 ataacgcaca aagtacatca tatccgctat ataatcatat caaaaaatcg cctgaatttc
     6121 aactcccttt gtacttttta tctatgttgt taaaaatcaa aattattttt gtgcaatatg
     6181 atatcttttt tattgaaatt ttacttattg cacaaattac attgacttga attgtttatt
     6241 ttgctaaaat gccatttgta caagttacac aatcacaccc gacggttatg tgacataaca
     6301 cccaaattat attctttatt cataaaataa gcttgaattt cggcaaattt tcctgatttt
     6361 agtaaaaaat gaccaatcac aaaacaaagg catttatttc aaacatcaca cattaatttt
     6421 ttaacataca gttgtaataa gtgctgacaa ttgttataat tataaagaca gcgaaaataa
     6481 gcaagccgac tttatgtcgg ggaaacggag atatatatga tgaaactgaa agaccttctt
     6541 ataaattttg aatacacggt tcagggcaat cttgataccg aaatttcgga tattatctat
     6601 gattcacgaa aggttgaaaa aggcacggcc tttgtgtgcc ttaaaggtta cacctcagac
     6661 ggacacaggt acgcaaagtc ggccgttgaa aaaggtgcat cggcactcgt tatcagcgac
     6721 agcctcgact ttgaagtgcc gagtgatgtt gccgttgtaa aggttgatga cacaagatac
     6781 gctctttcgc ttatgagcat tgaatttttc ggacatcccg aggatgagct ctacacggtt
     6841 gcaatcacag gcacaaaggg caaaaccaca acaacagcaa tggttgcgga aatttttgaa
     6901 cacgcaggaa tcaagaccgg cacaatcgga acactcggtg ttgtttacgg cggagaaacc
     6961 cacaaaaccg acaacacaac tcccgaatca tacattattc agaaatcgat gcgtgatatg
     7021 attaacgccg gctgcaaggc tatggtaatt gaggcatcgt caatcggact taaacacagc
     7081 cgtcttgcag gcatgacttt tgatgtcggc atctttacaa acttttcgga cgaccacatc
     7141 ggcggtgttg aacacaaaga ccttgcggaa tatctctact gcaagagtct gcttttcaga
     7201 caatgcaaat ttgccgctgc aaatattgac gatcccgcat ggaaggatat cacaaaggat
     7261 ttcaagggtt ctgttctcac ctacggtttt gccgacaatg cggacttgaa gggcaaaaat
     7321 gctcatcttc tctccgaaaa cggatttgtc ggtgttcact ttgaaacaga gggaatcaaa
     7381 aatctttctg ttgatgttga cattcccggc aaattcaatg catacaacgc acttgcggca
     7441 atttcggcag tttcattctt tgacgaaatt gaagatcaga tgattattga cgctctcaag
     7501 gttgccaagg tcaagggcag agttgagcgt gtgcctgttc cctacaacta cacactcatc
     7561 atcgactacg cacacaacgc actttcaatg gaaaatgtac ttgaaacact tcgccagtac
     7621 aatccgcaca gacttattac aatgttcggt gcaggcggca accgtcctaa ggttcgccgt
     7681 tatgaaatgg gcgaaacctc gggcagactt tcagaccttt cggttgtaac ggaagacaac
     7741 tcaagatttg aagatgttat ggacatcatt gaggacatca agacaggtct tgcaaaaacc
     7801 gacggcaagt atgttgtcgt gccgaaccgc agagatgcaa ttaaatattg catggacaat
     7861 gccgaggacg gagatattat cgtacttgca ggcaaaggtc atgaggatta tcaggaaatc
     7921 aagggtgtta agtaccatat ggacgagaga gaaatcattg ccgatattct caacggcaaa
     7981 agctaaaccc ttaaacatat aaatacagta aaaaatcacg gtcggaaatc aaattccgac
     8041 cgttttttca tactcttatt aagttaaata atcattccgc cgtttacacc gacaatctgt
     8101 cccgtgatga accttgccct gtcgctgcag agaaacacag cagtttcggc aatatcatcg
     8161 ggcataccga gcatattaag cggagtttcc tccttaagct cctgtacggt ctgctcatca
     8221 aagcctttca tcatatcggt cataatcaca ccgggacaaa tacagttgac acgcactccc
     8281 gacagaccga cctccttggc aagacctttg gtaagtccga ttacacccgc ctttgacgca
     8341 ctgtaatgta cctcacaaga cgcaccgacc tgtccccaca ttgagctgat attgagaata
     8401 acaccgctgt ggtttttaat catatatctt ttgagcgcct gttgcgagca gtagaaaaca
     8461 ccgtcaagat taacccccgt cattttcttc cattcatcgg gagttatgtc ggtaaacagc
     8521 ttttgctgtg caattcccgc attgttgaca agcacatcga ggttaccgaa acggctgtcg
     8581 atttcgtcaa acattttgca aacgctttca taatcggaaa catctgcctg aacagcaaag
     8641 gcatttatgc cgtattcatt tgtaagcttt tcggcaaggc tttcggcttt ttcggaattt
     8701 ttcgagtaat gcaacgcaac atcgcaaccg cattttgcaa aagcaacggc agtctgtgaa
     8761 ccgattccgc ccgatgcacc tgttacaagt atttttttca tcatattaat acccggtagg
     8821 ggcggttatc atccgcccgt tcgtgtgtat tgcacacgat tgaacacatt gtgttcaaat
     8881 ttaaacatat tattgattac atcaaaacca tatttaatga acacaaattc ttattaaagc
     8941 gggcggtcaa agaaaagacc gccctttgtt tattttactt cttaaaactg tctttaaggc
     9001 ttacactgcg gttgaatacg aggtgttcgg gtgagctgtc cttgttgtca atgcagaaat
     9061 agccgtggcg cataaactga tagctcttcg gagccgttgc acctgcaata ttcttttcag
     9121 ccttacagcc tgtgagaact tcaagtgaat tttcgttaag ataatcgagg aagtttctgt
     9181 cgcctgtatc gggatccgga accgtaaaga ggttatcgta aagtcttact tccgcatcaa
     9241 ggcagttgtt agcgtcaacc caatggattg tgcctcttac ctttctgccg tcgggagtat
     9301 ttccgccctt tgtttcggga tcatactctg cataaacctc aacaacattg ccgttttcat
     9361 cctttttgca gcctgtgcat tttacgatgt atgcagactt gattcttacc tcgttgccgg
     9421 gataaaggcg ctgatacttc tttacgggtt cttccataaa gtcggtttct tcaatgtaaa
     9481 gctcacggct gaaagtaacc tttcttgtgc cgtcctcggg acggttcggg ttgttctcaa
     9541 cctcaaattc ttcgctctgt ccctcaggat agtttgtaat aacaagctta acaggcttta
     9601 aaacgcacat tgtacgctct gctgtttcgt taaggtcatc acgcagacaa tgctcaagaa
     9661 agccgtattc aacaattgaa ttaacctttg aaacaccaat ctgctcaatg aacgaacgaa
     9721 ttgactttgg tgtgtaaccc cttctgcgaa gtccgcagag tgtaggcatt ctcggatcgt
     9781 cccaaccgct tacatagttc ttttcaacga gttcacggag ctttctcttt gacattaccg
     9841 tgttgttaat tccgagtctt gcaaattcaa tctgtcttgg cttgcacgga acggaaatat
     9901 tgttaattac ccagtcataa agcggacggt gagcctcaaa ttcaagtgaa caaaggctgt
     9961 gcgtaatacc ctcaagagca tcctcaatcg gatgagcaaa gtcatacata ggataaatgc
    10021 accacttgtc gcctgttctg tgatgcttga gtctgttaat tctgtagata acaggatcac
    10081 gcatattaaa gttacccgac gcaaggtcaa tctttgcacg gagagtcatt ttgccgtcct
    10141 caaattcgcc gtttttcatt ctttcaaaca aatcaaggct ttcttctata ggacggtcac
    10201 gatacggact ctttgcagga gtctgtgtgt cgcctctgta ttctctcacc ttgtcgggag
    10261 taagctcgca aacatatgca agtccctttt tgataagctc aacggcaaat tcatacatct
    10321 gatcaaaata ctgtgatgca tagaaaaatc tgtcgtccca atcataacca agccacttga
    10381 tatcctcttt gatagcgtca atatattcta catcttcctt tgtagggttg gtatcatcca
    10441 tacgaagatt gcagattccg ccgaaatgct cggcagtacc aaagtcaatg caaagagcct
    10501 ttgcatgacc tatgtgaaga tagccgttcg gttcgggagg aaaacgggtg tgaacctttt
    10561 taccctcaaa ttttccgccc tcggcagtat cctcttttat aaaatcccag atgaagttgc
    10621 cctgaacggc agcttcaccc tcaacattct ttatttcatc agccatttgc cgtgtacctc
    10681 cgtcagtaga attatgcgcc gagcttttca agaccgattt caagtctttt gagcgactct
    10741 tctcttccga gaatgtcaag aatttcaaca gcaccgcccg gagttactgt tttgcctgct
    10801 gcggcaattc ttacaggcca cataactgtg ccgtttttaa gctctttttc aaccgccata
    10861 ttgatgaggc aatcgtgaat tgaagtgttg tcccatgtcg gcagagcctt taatctttcg
    10921 taagcctcac gcaatacaac gggagcgttt tcaagattag tcttgctctt cttatttacg
    10981 aaaagctcct tgtcatattc gggaagttct gcaaagaaac cgagcatttc gggaatatcc
    11041 gtaagctttg tagttctttt ctgaagaatt tcggcaaact tctcaaaagg catttctctg
    11101 tcaccgaaaa ccttcttata gtatggcata caaacatccg taaattcttc gggagtcata
    11161 gcctttaaat actcaccgtt gaaccatgaa agcttgtcat agtcaaaaat tgacggtgac
    11221 ttgctgatac cgcttacatc aaactctttt tcaagttctg caagggtaaa gatttcctga
    11281 ttatcctttg gacaccagcc gagaagtgca atatagttga tgattgcctg tgggaggtag
    11341 ccctcgttaa taaggtcata gaagcctgtt gcgccgtgac gctttgaaag ctttgaaaca
    11401 ttgccgtcgg catccttgcc cataatgagc ggaaggtgga tatatgtcgg aatttcccat
    11461 ccgaatgctt cataaagaag attatatttc ggtgtggagc ttaaatattc acaaccacgg
    11521 acaacatgag taatgcccat tgtgtggtcg tcaattacat ttgcaaagtt gtatgtcggg
    11581 tagccgtcag tcttgataag aatctgatcc tgaagttcgc tgttgtcaac ggtaatttca
    11641 ccgaaaactg cgtctgtaaa tgttgtactg ccctcaatcg gcattttctg gcgaattaca
    11701 taaggtgtac cctcggcaag aagtctgtcg atttcttcct gcggaagatt acggcaatgt
    11761 ctgtcataac caccgccaac gctgtcttcc tgtaactttt caagtctttc ctttgtgcag
    11821 aaacaacggt aagcctttcc ctcttttata agctgttcgg cataaggaag atacatatcc
    11881 tttctttcgg actgaacata aggaccgaaa tcaccgccga tatcgggacc ttcatcgtgt
    11941 ttaagtcccg caagtttcat tgtatcatat ataacatcaa ccgcaccctc aacaaggcgt
    12001 tcacggtcgg tgtcttcaat tctgagaaca aatttgccgt tttgtgattt tgcaacgagg
    12061 tactcataaa gagcagtacg cagattgccg acatgcataa aacctgtcgg gcttggtgca
    12121 tatcgtgtac ggacagtttt ttcagccata ataatatccc tccgaaattt atgtttaggt
    12181 gtgtcggtca gcgcatattt cgcccgcaac accgttgtta catatatacg atttattata
    12241 tcataccgtt ttttcatttt caagtccgat gaacgcacct acgaacaatc cgaaaatttc
    12301 atataataaa aattgcatca gaacctttct cgggcatctg atgcaattca tttatctaac
    12361 tatcttctcg aacagacagc ggtggcacac cgccgaccat aacttttaac tattcacttt
    12421 tcactgctga cgaaattagc ctgcatatgt tcccatatac agcggaatgt cactttcgtt
    12481 gtcgaggata acaaacataa acggacgatt aaattctacc gtcacatcgg tttttgaaag
    12541 ttcttttgtt cttttttcaa gctctgcctt gtcgccgttt gactgtgttc cgccgatacc
    12601 gcctgcgtta attgaaagcg acggagcgat gtcatacatt gcgctgaacg caatatcatc
    12661 tgaacggctc agattcttgt atcttgaacg gtcagccgtg aatgatgaat tgattccgct
    12721 tttttcaaca atctgcgtta tattttctgc agacttatct ccgctgactg aaaattcagg
    12781 aatttttgcc ttggctgttt tggtcacatc aacgctgtca agcaatgttg tgtattcaag
    12841 gctcgtaaaa tcggttgcgt aatcatcaag cgaaacacct tcgttcggca taataaccat
    12901 cattttgaga ggtgtttgcg agaaatattt cataacggca gtcgccttgt ttgtcttcat
    12961 atatgtttcg tttgaggtca tatatgtaac ttctttttca ccgtcagccg acttaaattt
    13021 agccttttca atgtcggtct gtgcataagg attaagccat ctgtcacaga tatctgtcgc
    13081 agaaagtgaa atcacactct gctttgtgtc aagattttca aaaacagaac cgttgtttgt
    13141 aaaatcggca tattttttgt caagcttttt caaagcgtcg ctgtccgaaa aattaaaacg
    13201 cagaacatta gtatcataat agtcctttgt tgtctgcaaa aagcctgtca taatgtcggc
    13261 attgtcattt gaaatcagcg agttgcaaag ctttacaaag cttttgtcgc tgttcttttc
    13321 ggaatctttt tccgagcttt gcgagctttc gggcgatttg tccgaagatg caaccgattg
    13381 aatacggctt gcaaaatatg acgcacattg gttcatattt tcaagcgtca caccgctgca
    13441 aagtgcattg gtgatttctt cctgagtatc gcccgacgca ccgttagcca tagcctcaag
    13501 attaagaacc gcatttgcgg gacaaaatac atacgaaccg cttttgtgac ggtttctgaa
    13561 aagcctgagt tcaaaatcgg ttatttttgc ggaataggta tcatatgatg acggggcggt
    13621 ttttgcaccg tcggcataat tgaatgtatc atcggttttg tcggagcgtg tgtatttcag
    13681 cgaaaggttt tcggtgtccg aaaattcatc gggatttgaa cacgcagtaa gcaccgtgcc
    13741 tgccataaga atacacaatg caacagagag tgcttttttc atcatttgtc atttcctcct
    13801 ttggaatcgt tacaaagctt ttgcgccagt ctgaatattt tttctgccga atcggggcgg
    13861 tgcaactctc tgcacttttc cttcatctca tcaagttttt ctttgtcttt aacaaggttt
    13921 acgatatcat ctgcacctgt cttttttcgg agcataattg ccgcaccctt gttgagtaaa
    13981 aattccgcat tgtcccgttc ctgacccgga aatgcgctgt agattgccat gggaagcgaa
    14041 caggcaaggc tttcggtaac ggtaagtccg cccggctttg taacaataag gtctgagatg
    14101 tgcatataat cctcaacatt ttttacaaag tacaaaagct ttgtgttgtc ctgcataccc
    14161 gtttcttcaa ttaccttttc aaggtttgca aaaagcttga tgtttctgcc ggtgatgaca
    14221 ataaactgca tttcgggagc ttgctccaca agagctttgt agaagctcag cacgcttgta
    14281 acaccgaaag aacctgccat aagcagaatt gtaggcttgt taggatcaag cccttcacgc
    14341 tcacaaatcg cctttttgtc aaaaggctct gtaaaacggt caaaaatcgg aatgccgagc
    14401 ggatatatta tgctcttgtc aacaccgtat tcatcaataa gctttgtcgc catatcaggc
    14461 tctgcaagca catatgcgtc aacatacgga acaatatatg ttctgtgtgc gtcatagtca
    14521 gttataaggc tgattgcctt aacatcaatc ttgtgctgac ggcgaagctt tgaaatcatt
    14581 gttgtaacaa acggatgaca cataataatt gcatcgggct tgtattcatt gattgtgtca
    14641 agcagctttg ccgacatcat tgtgttggac tgcataacag ccttgtacat aagggttctt
    14701 ctgtcggtaa tcttatagaa tttgccgtaa acctttggaa ttttcgttgc cataaaatga
    14761 tagccgtcac aaacggtttt atcatatacc ctgccgattg tcttcatggc gtcaataacc
    14821 ttgaccttcg tgtcgggact gacggctttt atttttgcct caatagccgc agcggctctc
    14881 ttgtgccctc cgcctgttga tgctgtgaag atgattattt tcatatcagt tctccgtaat
    14941 tcaaaataac cgaaaacggt tttgcacaca ggggtgcttt tactattata cttttaatct
    15001 gcaagtattt caatagatta tttttgcatt tcatctgaat tttaaataaa aataattaaa
    15061 ttttcgtttg tgttttttaa tcggggcaac tgttccgggc ttttaaggcg gtaaaaattc
    15121 acactcggaa tatgagtcgg aaatattgct aaaatctttt ggtaaaaagg acttaaattt
    15181 ctataaaaaa tttttctaaa ttttgtgttt tcacacttta tttttttaaa attatattgt
    15241 aaaataaaaa ctgtactgta atatatttca gtatagggta attaaagaaa gtaataaaag
    15301 cgtttccctt tttgaaaatc aaatgcagta ggttttcaaa gggcaattat tgggtatata
    15361 tgaggtgaat ttttttgaac aggaatttta tggacgatat tcttgttgtt gatgaagatc
    15421 ttgttgacat caactgcgac ggcaacagac agtctgataa caagaaagga aaacattcat
    15481 cgggcggtcg tatggctaaa ggcgaaaaga aaagcaagaa aaagcttgca atcatcattt
    15541 catccgttgc cgcagttgca gtagctctcg gtgtggcagg tttctgcatt ttcggaggaa
    15601 acctgttcaa cagcgttgag gaggctattg ccggtgagtt taagtttcct gacggaacaa
    15661 ccgtttcggg tgtttcaatt tcgggaaaaa catacgacga ggcaaaaaaa gctcttgaag
    15721 aaaaggaaga aagctttatt aagcctcttg acatttcggt tgatgtaaac ggagtcatca
    15781 gtaaagtgac cgaaaaggat ttcaaatata cttatgacat tgagtctgta cttaatgaaa
    15841 tcaaaaatga agcaaccgat ccgtctgttg aaacttcaac aagcaaaaag agttacacgg
    15901 taacagctac cgtaatcgca gaaagtgttg acgaagcagc aaagaaggtt gcaaagaaaa
    15961 attacaaaga ggctgagaac gcaagagttt caaagttcca cccctatgcc aaaaagcgtt
    16021 ttgaatatac agaggcgtca cagggacaga aagtaaacga aacagacctt gcaaatcagt
    16081 ttaagggtgt gtttgcaagc ggtgcaagcg aatacagaat tattgccgat gttgaaaaaa
    16141 cagatgcaaa aatctcggtt gacgatttga agaaaaacat cgttcttctt tcaacatacg
    16201 aaaccgtttc caccaatacg gaaaacggaa cagaaaatat gcgtgtttcg ctcaaagcct
    16261 gcaacggttc ggttattgaa cccggtgcaa cttggtcatt caacaaatgc acaggcaact
    16321 caaaccttga atctctcggc tacaagccgg caggtgtaat ttcaaacggt aagtccgaca
    16381 tcggtatcgg cggcggtatc tgtcagtcaa gttcaacaat ctacaatgcg gctgtcagag
    16441 ccaacatgaa ggttgaggag cgttattgtc acaagtgggc atcaagctat gttcccacag
    16501 gtcttgacgc aacaatcgac tacggcaacc ttgaccttaa actttctaat ccgacagatt
    16561 accagatgtt ccttgagtgt aaggttgttg acaacacact ttatgtttca ttctggggtt
    16621 ggaaatcgga ttcatacgac cttattatga ctcgtaacaa gctcacgaat cagggtagtt
    16681 caagctacac ggtaaaggct tggagagttt attacaagga cggcaaggag attgattccg
    16741 agtcactcgg ttcatcaact tatgataccg aaaacggata tgttttcatt gatgccaaca
    16801 atgatccccg tgcaaaatac ggtgatgatg ttgtaatccc ggatgaaaca gttccgaaga
    16861 atgacgacag cagttcaagc tcatcaagtt cacagtcatc atacagtgaa ccgtcatatt
    16921 caagcagttc ttcatcaagt cattcaagca acagttcatc atcaagctca tcgagccaca
    16981 gttcatcgag ccacagttca tcaagccagt caagttcgga atcaaaaacc gagccacagc
    17041 caaaacccga tcccgaaccg acgcctacag aacctgaatc aggcgaagaa taattaagca
    17101 aaacaacttc cgataataat aaaaaactcc ctttcgcaga taataaaagc ggaagggagt
    17161 ttttgtatga taaaacgaac aggtgcatat acaattgctt ttgaaagcca gcctgttatt
    17221 gccggctggg ggtcggttgt cggaaaaaag gaagccgaag gtccccttaa aaactatttc
    17281 gacaagatta tttacgacag ctatgacggc tgtgacacct ttgaacaggc ggaaagtatg
    17341 tttcagggcg aagctcttga aaaagctctg gagcgttcaa aaacacacgc aaacgaggtt
    17401 gactgcgttt tcgcaggaga tttgctcaac caatgcatcg gatcaagctt tggacttatg
    17461 aaattcggaa tcccgtatct tgggcagtac ggagcgtgtt caacaatggc gcaaagtctt
    17521 attatggcgg catctgccgt tgaaagcggc ggagcaaaag tttccgcctg tgtcacctcg
    17581 tcacactttg cgtcggcgga acggcagtac cgttttccgc ttgaatacgg cggagttcgc
    17641 actccaaccg cacaatggac agttacgggt gcgggaagct gtattctgaa aagccttaaa
    17701 aaaggaatct gcgttgcaag agcgacagtc ggcagaatag ttgacctcgg tgtaagcgac
    17761 gccaataaca tgggggcggc aatggctccg gcggcagctc agacccttga atgttatctt
    17821 gaggatacca aaacatcgcc cgaggattac gatatgatta ttacgggtga tttgggcgaa
    17881 gtcggcagta aatcacttta tgatttgctt gagcgtgacg gaatcaatat aaggaaacaa
    17941 cacaacgatt gcggactgat gatttttgaa cgctccaatc aggatgttca tgcaggcggt
    18001 tcgggctgcg gatgcgccgc atcggttctt tgttcaaaaa ttatggacga ttttcaaaac
    18061 ggactcgttc aaaacatcct gtttatggca acgggtgcac tcatgagtcc gacatcaagc
    18121 ggacaggggg caaatatccc gtcaattgca catttggtga atctgaaaat taagtaaaat
    18181 ttttgcaaaa tatctgttca aatgaatttt aatgtgttat aatgaacctg tatgagtaat
    18241 gatagaatgc tcaatcagcc tgttttgcaa gcatattttg cgacagacag gataaatatt
    18301 acgaacagga gttgtgttta tgtttggcag aattcaaaac attgacaaca gggtacttga
    18361 taacatatcc cgaattcata agccggcact aaacaaaata atgattactg catctcgtgc
    18421 aggcaatgca ggtattgtat ggtgggcaat ctgcctgccg tttcttataa actccaactg
    18481 gagggcaaca ggtgcaaact ttgtgttcgg acttgcgtgg gctcacctta tgggagagat
    18541 tattatcaag catatagtaa agcgtgtgcg accttgccac acccttgatg atgacgaaca
    18601 gcttattgac agaccgagat tctactcgtt tccgtcaggt cacacgacgg catcgtttgc
    18661 aatggtcggg gttgctctta tgcgttgcaa agtcataacc tttatgccga ttcttatgct
    18721 tgcaatgttg attgcgtttt caagaattta tctgcgtgtt cactacctta cggatgttat
    18781 cgccggtatg attttaggct taacctgcgg aatctgctct gtccttattt tcaataccgt
    18841 agttccgatg tggtttccta tggtataact tttgaaaaaa taatatgata aatgaattgc
    18901 atcggtcgcc cgaacaaggc tccgatgcat ttttgtttgt acgcataact tttgagtgac
    18961 gaaaacagta tgtaatatgt ggcaataaca ttatgttata aacatccttt gctaatcaac
    19021 actcttatcg gtttatttat ttttctgcaa ttatctataa caaatttagt acctctgctt
    19081 tttccgtccc agaaagccaa aacaaaatct gcattgtcaa tgatttcaag attgcgtttc
    19141 agcggagcat atcttccatg tattttatat tcaggaagaa attccgtgta tctgatattg
    19201 ttttttaccg caaattcctt tgcacatctg tcaattccat ttgcacctcc cgaaatgatt
    19261 tctgttgtat ctttcggaat atatttttca agatttctga ctgtaagcgt ccttgaaccg
    19321 ataaccgcaa ttttcattaa caaagcactc cctcttttta tgaacccatt atgggttcac
    19381 ttttacatta tagcacaata aggacttata ataaacccat aataggttca tttgaggtgc
    19441 aattttatga aaggaaagcc aaaacatttg tcactacgca ttgatgaaga tttattgcgt
    19501 aaatttgagt atgtcgcaaa gtatgatgac aggtcaatga attggtatct tcttcatctg
    19561 attaaaaatg aaattgcgga ttttgaagaa aaacacggtg aaatccccac cgcctccgac
    19621 agtaaaacag aataatcaaa attaatttaa ggtcgaaaaa gcaatttttc ggtctttttg
    19681 tttgcctaca tattctttga tgtaatgaat gttatgtttt aatttggtat acacgattgt
    19741 acgcattgcg tgcaatcgtg tgcaatgcac acgaacggga gaacacggtt ctcccctaca
    19801 tttaaacaca acttctatat ataaaaagca gtcgggcgac aatgatttgc attttgcaaa
    19861 ctgtctgccc gaccacaatt tttaattttc cattttcaac tttcaattta aaaaaacttc
    19921 cgcttgagca taatatgttt ttaatattac aaactgtaac agcaaagcag tcggttggca
    19981 atataccaac cgactgactt tttaattatt cattttcact ttctacccga gtaatttatt
    20041 actcacgatt actcataaag cggatatttt ttgcagattt cttcaacacc tgctctaacc
    20101 ttttccttgc tgttctcata gtcgttaaga acgagggtga tgtactcggc aatcttgtcc
    20161 atatcttcct cgttaagacc tcttgtagta acagcggcag taccgattct tacaccgctt
    20221 gttacgaacg gtgaacgggg ttcgcccgga attgtgttct tatttactgt gatgtagcaa
    20281 tcgtcaagac gagcctcaag ctccttgccg gttacatctg tatcacggag atccataaga
    20341 agaacatggt tgtccgtacc gcctgaaaca agcttgttgc ctcttttgag aagtccgtct
    20401 gcaagagcct tacagttaga aacaatcttt ttgccgtact ccttgaactc aggctttaat
    20461 gcctcgccga agcaaacagc ctttgcggca ataacatgca taagaggacc gccctgtgta
    20521 ccggggaaga tagccttgtc aatctgcttt gcaaattctt cacggcagag gataagaccg
    20581 ccacgaggac cacggagagt tttgtgtgtt gttgttgtaa cgaactcaca atacggaaca
    20641 gggttcggat gaacgcctgc ggcaacaaga cctgcgatgt gagccatatc aaccataaga
    20701 tatgcgccta ctgcgtcggc aatctcacgg agctttgcaa aatcaatgat tcttgggtat
    20761 gcagacgcac ctgcaacaat catcttaggc ttgcattcct ttgcaatttc aagaacctta
    20821 tcataatcaa ttctgtgcgt ttcgggatca acaccgtatg gaacaaaatt gaagtattta
    20881 cctgaaatat taacaggtga accgtgtgta aggtgaccgc cttcgttaag gttcataccc
    20941 attaccgtat caccgggatt aagcattgca aaatataccg ctgtgttagc ctgagcgcct
    21001 gagtgaggct gaacatttgc gtgttcggca ccgaaaagct cacacgcacg ctgacgggca
    21061 atttcttcaa ctacatcaac gcactgacaa ccgccgtagt atctctttcc cggataaccc
    21121 tcggcatatt tgtttgtgag caagctgccc attgcagcca taactgccgg agaaacgatg
    21181 ttttcacttg cgataagttc aaggtttctg cgctgtcttt taagctcgtc tgtcattgcg
    21241 ttaccgattg cgggatcgta ctcgttgagg taatcaagag tagcgatatt tgttttttca
    21301 ctcattgtta tattcccctt atatttttat tttaggatac tgtgtatcct ttttgaattt
    21361 atgtcattgc aacgaaaggg ctttttgcct taattcgtac aaatcatcaa gcgttttaat
    21421 ttttcctgcc tcatttctca aggctgccgc acccttcatg cctttgaaat agccgactac
    21481 aagttttctt gcctgaagca ttgccatatg ctcgcccttg tattcaacca ttttgccgat
    21541 atgttcaatc attacaccga gcctttcttc aaggtcggga gtgtgaattt taacattcgg
    21601 gttttcaagg tatgcgttaa tctgcgagaa aatccacgga tttccgagcg ttgccctacc
    21661 gatcataaca aggtcacagc ccgtgatgtc cataacctcc tttgcccttt cggcactgtc
    21721 aatgtctccg tttgcgatta cgggaacgct gacggattct cttacagccc ttatgatatc
    21781 ataatcaacg ggaggcttgt aatattgctg tcttgttctg ccgtgaacgg tgattgccgc
    21841 cgcacctgcc gattcgcaga tttttgcaac ttcaacggca tttacgctgt catcatccca
    21901 acccttgcgg atttttacgg ttacgggtat gtcggtaacg gctgtcacag ccttgacgat
    21961 ttctccgcaa agccgaggat ttttcatcaa tgcacttccg ctgccgtttg agcttatctt
    22021 tggagcaggg catcccatat tgatatcaat aatatcaggt ttgttttcaa gtgcgtgctt
    22081 tgcggcatca gccatacatt tcggatcatc accgaaaatc tgaattccgc aaggacgctc
    22141 caagtctgaa atttccatca gttcttcgct ctttttactg ttgaaagaaa gcgcctttga
    22201 gctgaccatt tcgcttacgc agtatgccgc accgaatctt acgcaaagct cacggtaggc
    22261 cctgtcgctt acccccgcca tgggtgcgag aactgcgtgg gatttcagcg gaacaccgcc
    22321 gatgttaaga ttatccatat ttttacacat ttaatgctgt taccttattc tttttcttgc
    22381 accgactttt gtgctgatca ttacaaccgc aattgcaact actccaagac ctacgcaaat
    22441 ccacgaaacg attcccgccg tgtagcttgt ggaatacttt ttttcgctga ctgtttttgc
    22501 gattgtcgga gcctgaaatt ccgttgaatc ggtcgcctta gtttcaacat actgattctg
    22561 atactgcgtt tcttcctgtt tctgagcttc cgtttcgggc tgaacatact cagttgtggg
    22621 ttcttccgtt tcgggctcaa catactcggt tgtaggcgct tcggtgtaaa caggctcggt
    22681 ataaatcggc tctgtgtatt gcggttcggt ttcaacggga acatactccg tcactacggg
    22741 atcgggctgg ggatcgggag tttgcgaatc gtcctctcct accgcaaagg cggttattga
    22801 acaaaccgac aatgcaagaa ccgcaagtac ggtcggaagt attcttttcc taaaaatttt
    22861 cttaaaatct gacttcatat ttaatacccc gttcattctg cgtggcacat attttcatat
    22921 acatactacg attataacaa aagcaggcgt tcattgcaag atttcagaca acttttctgc
    22981 acacaattaa tgcccaaaca acaaaaaatc ggaaaaccga ttgtaaaacc gattttccga
    23041 cttgattatt atgtcagttt tttgagcgtt ttttagcctt tggcataaca agattgtagc
    23101 tttcgtcaat cagcggaaag atttcttcct tgctcacaga gccgtcaagc agaatactta
    23161 tccacttgtc cttgttcata tgataggctg gcagaaatcc gtttttcatt ctcagcgagc
    23221 cgacaagaat cggatcgcac ttgacattca tcacatcgac aaagccatcg ccgtcaagcc
    23281 ctaaaatctg ccttttgaca cacataatta ttgcgtacca tttgcccgac atcatattcc
    23341 gcagaaccgc attatcggga ctgctctccc acggatattc gggttctgtt ccgtatgtgc
    23401 taaaggcata atttaaaatt acctctctgt taatagaatt atcattgtat gccattaagc
    23461 cttaactcct gcttttttct taacgagtga aatatattcc tgcattgtga tttcgttacg
    23521 cagtaatttt tgacaccatt ctttgctttt gctgtcaatg gtatagcctt ccatttcaac
    23581 tgatgcggca gcattttcta ttatctgttc aatcggctta ttaccgttca cagtaacact
    23641 ccctttcgtg aaatctgaac ttcatatcat tttatcataa caaaggcata tgtcaaccaa
    23701 aaggtgaaaa tcattcgcca aagctgatgc ccatttttct tgctgtttca acaacctcgc
    23761 tgtctttcgg aacgctcttg agcttgcctg cgatgtcctc aagcggaacg gcaactacct
    23821 tattatttac aagggcaacc attttgccgt agtctttatt gataataagc tctgcggctt
    23881 taacgccgaa acgagttgaa atcacacggt cataggcatc gggactgccg cctctctgga
    23941 agtgacccgg tactgtaacc cttgtttcct gacctgttgc ctcctcaata tcatgggcaa
    24001 tctgatagga aattgacgga tatttcatct ctgcaacagc agcttttctc tgctttttag
    24061 gcaaagctgc aatttctttt gaaattgcac cctcggcaac tgcaagaatt gaaaagttct
    24121 taccgcctgc ggttcttgac ttgattgtct ttacaaccga gttgatgtcg tacggaattt
    24181 cggggataag gataatatcc gcaccgcctg caatacctgc ataaagtgtg agccagccaa
    24241 ccttatgtcc cataacctca ataatgaaaa ctctgccgtg agatgttgcg gttgaatgga
    24301 tacagtcgat tacatttgtt gcaatatcaa cggcactctg aaaaccgaat gtcttgtctg
    24361 taccccaaag gtcgttgtca attgtcttag gaagcgaaac cacattgagt ccctcttcac
    24421 gaagaagatt tgcggttttt tgagttccgt tgccgccgag tacaacaaga cagtcaagac
    24481 caagcttttt gtagttcttc ttcatagcct caaccttgtc aacgctgttc tcgtcaacaa
    24541 cacgcataag cttgaacggc tgtcttgatg tgccgagaat cgtaccgccc attgtgagaa
    24601 tacccgaaaa attctcgggt ttcatctttc ttgtttcgcc gtagataaga cctctatagc
    24661 cgtcaataat gccgatgatt tcaacatcat tgccaagctg attataaagt gtttttgcaa
    24721 cggcacggat tgttgcgtta agtccctgac aatcaccgcc gcttgttaaa atgcctactc
    24781 ttttcattgt tcggagtcct cctttttcac ttcaacgggt acaatcggag ttccgacagt
    24841 gctcgcatat gtctttttca ttgcctgctt ttgagccttt aacgcaagct ttgaaaagaa
    24901 tttctgacaa tttgttttgt gttttccacg cttgtcaaca gacttatata cacctgttga
    24961 aagctgttgg cgtgcattta cggtatcctt aaatgaaacc gtgataacct cctgcacacg
    25021 ctttttgata tcctcgtcct caacagggaa cacaagctca actcgtctgt cgaggtttct
    25081 cggcatccag tcggcacttc ccatataata aagcggattt gaattgtttt caaatatgaa
    25141 tattcggcta tgctccaaaa gctgaccgac aattgaaatt actctgatgt tttcgctgaa
    25201 tccatgaacc cccggaacaa gacagcagat acctcttact atgaggtcaa tttttacgcc
    25261 tgcgtttgac gcatcgtaca aaaggctgat gatttcagga tcaacaagcg agttcacctt
    25321 tgcggtaatt ttggcaggaa gtccgagctt tgcgttctcg gtttcctgaa taatcatagc
    25381 ctcaaagaac ggacgcattc cgtgaggtgc aacgataaat ttgttgtatt caggcggagt
    25441 tgagtaacct gtcaatacat taaagagtga tgatgcgtca acaccgtatt cttcacggca
    25501 ggtgaacata ccgatatcgg tatagaatct cgccgtactg tcgttatagt tacccgtacc
    25561 catatgcaaa tatcttctga ttccgtcctt atcttttctt actacaagac agattttgca
    25621 gtgggtttta agtcctgtaa gaccgtaaat tacatgacag cccgcctttt caagctttgt
    25681 tgcccactga atgttgtttt cctcgtcaaa tctcgccttg agttcaacaa gaacagttac
    25741 ctgcttgccg ttttcagctg ccttgataag agccgcaaca atcggtgaat ttccgctgac
    25801 acggtacaag gtttgcttga tggcaagaac attcggatcg gttgccgcct ttttgacaaa
    25861 atcaaccaca acatcaaagc tttcataagg atgatgcaca agtctgtcct tttcacggat
    25921 agcctcaaaa atatcgtcat aaccgcagaa ttccgcaggc ggatttacgg gaacgattgg
    25981 ttcaaagcac atatcctcac agcccttgat gtgggcaaat tttgaaagga atgtaaggtc
    26041 aagaggacct ctctgaaagt agatttcacg ctccgtaaca tcgagagcgt caacaagaaa
    26101 ttcacgaatt tctcggttgc atttcttgcc gaattcaaga cggacaggtt ctcctctctt
    26161 acgctttctg attgactttt taatttcagc cataagatcc tcggtatcct cgtcaatgtc
    26221 aaggtcggaa tctcgggtga ttctgaaagg atcgtactgc ttaatgttgt aaagctcaaa
    26281 aagctcgccg aggtgcattg cgattatttt ttcaagcaga ataaacgctc tgccctcgtg
    26341 agtcggaagc tcaagaaatc tcggaagaat tgacggaacc tgaaccaatg cgaaaaattc
    26401 ttcattctcg gcatttgtaa ggcgaaccgc aatgttaagg cttttgtttg caagcatcgg
    26461 gaacggacgg cttgtatcaa cagccatagg cgtcaatacg ggaaaaacta ctttttcaaa
    26521 gtattcctca acaaacgctt tctggtcgcc gtcaagctca tcagcctctt tgaacacaat
    26581 gcctgccgaa cgaagtgacg gaattattga acggttgtaa cagctgtact gctttttggc
    26641 aaactcgtga attttttcaa caagtccgtc cataacctct gtaggagtca tgcccgatgc
    26701 gtcacggctt gttctgccgt gatgaatctg gtcaagcaca cctgcaacac gaaccataaa
    26761 aaattcgtca aggttcgatg ccgtaattga aaggaagttg catctttcca taattggatt
    26821 ggtctttttg aacgcctctt caagcactct tgcattaaag tcaacccagc ttaattctct
    26881 gttagtataa ggcatctttt gccattttga cataagcttt gctttttcgg atgttttttt
    26941 attgttgttt tttgaatttg acatataatt caccaccata aaaactgata tataacccaa
    27001 tttaatatat ctgattaagt ataaaatacg aaacggatta tttcaagcga attgcactaa
    27061 attttacctt tggcaacgca ttctttacat atattttaca ataacacaaa taaaattgat
    27121 ttgcaataga tttgacaaaa ttcgcaaaga taaaaccaaa attttctttt tgcaaaatat
    27181 tcaaaaaatt tcaaaaaata ttgttttaat ctctgtatta tgttatactt gcgttgattg
    27241 ttttatttac agattttaaa aaattctaaa gaaaatgaga ggtaattatc atgagtaaaa
    27301 aaaggggaag cttttccggc ggactcggat ttgtatttgc cgctgcggga agtgcagtag
    27361 gcttgggaaa tctctggaga tttccgtatt atgcagctaa gtacggcggc ggaacattta
    27421 tctttatcta cataatcctt gcgcttacat tcggcttttc acttttgact gccgaaatcg
    27481 caatcggcag aaaaacaaag ctaagtccga ttctcgccta caaaaagcta tgcagcaaat
    27541 tttcgttttt aggctacctt gcaacgcttg ttcctataat cattattccg tactactgcg
    27601 ttatcggcgg ttgggttaca aaatatatgt gcgtttatgc acagggactt gttgcccctg
    27661 cggcacagga caactttttc tcggacttta tttccgaccc gttgtcaccc attgtgtttt
    27721 tcttaatatt tatgctgttg agtgcagtgg taattatgct cggagtaaac aagggcattg
    27781 agcgactgag caaatttttg atgcctgttc tgcttgttct gacagtcgga atttcaatct
    27841 acacactcac gctcccaaac gcaatggagg gtgtttcgta ctacctcatt cccgatttca
    27901 gcaaactgtc aatttggaca tttgcggcgg cactcggtca gctgttcttc tcaatgagca
    27961 ttgcaatggg aattatggta acctacggtt catacacaaa agatgaggta aacctcacaa
    28021 agtcggttaa ccaaattgag attttcgaca cagccgttgc acttctcgca ggtcttatgg
    28081 tagttcctgc ggtttatgta ttcagcggtg aagaaggtct tggcgcaagc ggtcccggac
    28141 ttatgttcat cacacttcca aaggtattca acgaaatgcc tgccggcgta atcatcggat
    28201 ttttgttctt tattcttgta ttctttgcag ctatcacaag ctcaatctcc gtaatggaag
    28261 caattgtttc ttcgcttatg gacaagttca agcttacacg aatcaagtcc tgtcttatcg
    28321 ttatcggcat ctgccttatt atgggtattc cgtcgtcact cggcaacggt atatggtcaa
    28381 atgttaagat tctcggaatg gacttcctta cattctttga cttcttgagc aacagcatta
    28441 ttatgccgat tgtcgcactt tgcacttgta ttctcatcgg ctgggttatc aagccaaaga
    28501 ctattgcaga cgaagtcaca aggaacggcg agcgtttcag cagaagggct ttgtttgatg
    28561 ttatgattaa atatatagct ccaatctgcc ttgtgttcat cttaatttcc tacacacttg
    28621 cgcagttcgg cattatttca ctttaacacg attaattatg aattcaaaca gtaatatcac
    28681 cgaatgaatc ataagtaatt gtctttaaat taagcataca acaattaact attctccgtt
    28741 tatcccatga actcttgaaa ttatatgcaa taattggtat aattacagtt acagctcatt
    28801 tgctgaattt tagggaggac aaatactatg tgtcagaaaa aaccatttta cattacaacg
    28861 cctatttatt atccgtcagg caatccccac atcggtcatt gctacaccac cgttgcgtgc
    28921 gattctatcg cccgttacag acgaatgcag ggatacgatg ttatgttcct tacgggtacg
    28981 gatgaacacg gtcttaaaat tgaacagaag gctgccgaaa agggtgttac tcccaaagag
    29041 tatgttgacg aaattgtaaa aacctttaaa ggcttatgga aatttatgaa cataagctat
    29101 gacagatata tccgcacaac agacgattat cacattgaaa cagttcagaa aattttcaag
    29161 gagctgtatg acaagggcta catctacaag ggagaataca agggcaaata ctgcaccccc
    29221 tgtgaaagct tctggacaga aagccagctt attgacggca aatgccccga atgcggacga
    29281 gaggttacag aggcaaagga ggaggcatac ttctttaaga tgtcatcttt tgccgacaga
    29341 attgaaaagc tcctcacgga aacagactat ttacagccaa agacaagagc ggttgagctt
    29401 gtaaacaact ttatcaagcc gggacttgaa gacctttgcg tatcgagaac aaccttcaaa
    29461 tggggcattc cggttacatt tgacgaaaag catattgttt atgtttggat tgacgcactt
    29521 tcaaactata tctccgctct cggctacaag aatgaaaaat ttgacgaatt tgacaaatac
    29581 tggcctgccg atgttcatat ggtagcaaag gacatcatgc gttttcacgc tatcatctgg
    29641 cctgcaatgc ttatggctct tgaccttccg cttccaaagc atcttgccgt tcacggctgg
    29701 attactttca acggacagaa gatgtcaaag tcactcggca atgttgttga tccgtttgta
    29761 ctcggcgagc gttacggcgc tgacgcaatc cgttaccaca ttatgcgtga aatggctctc
    29821 ggtgcagaca gctcattctc aaacgaaatt atgattaacc gtatcaattc cgaccttgca
    29881 aacggtcttg gcaaccttgt ttcaagaaca gttgcaatgg ttcagaaata tttcggcggc
    29941 acacttccca cagagcgtga gagcggcgaa tttgacgatg accttatcga aaccgcaaca
    30001 tcgctccgtg caaaggttga cgatttcatg gacaagactc agctccagaa cgcacttgcc
    30061 gaaatattca agcttgtttc ccgtgcaaat aaatacattg acgaaaccgc tccgtgggtt
    30121 attgccaagg acgaaacaaa gaaagcaaga ctcgcaacgg ttctctacaa ccttcttgag
    30181 gcaatccgta ttgcctgcac ccttctctcg gcatttatgc caacaacaat gccaaaggct
    30241 cttgagcaga ttggcgcttg tgaaaaatgt gccggctacg aaaactgcga caagttcggt
    30301 gttcttcctg ctgatgtcac agttcacaag ggagatgcac tcttcccacg aattgatgtt
    30361 gaaaaggaaa ttgaagaact caacagcatt atcaagaaca acgagggtga aagtgaagaa
    30421 acaaaggcac tccgtgaaga gcttgagggt gttgcacaga ttggcattga tgactttgcc
    30481 aaggttgacc tccgtgtgtg cgaggttaag acctgcgaac ccgttaaaaa gagcaaaaag
    30541 cttttacagt tcacaatttt tgacggtgta aaggacagga ccgttgtcag cggtattgca
    30601 aactactaca agcccgagga gcttatcggc aagaagatta ttcttgttca gaacctcaag
    30661 cctgcaaagc tttgcggtgt tgaaagctgc ggtatgattc tttctgcagt tcacggcgat
    30721 gatttaaagg ttgtttttgt tgacaatatg cctgtcggct caaaaatctg ttgatttatg
    30781 ttcgtgtaga cagacggcga gccgccgttc ccctttcgtg tagacagttt gataaattaa
    30841 aaacc
//
LOCUS       DAOMVE010000004        29889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_217_length_29889_cov_3.310149, whole genome shotgun sequence.
ACCESSION   DAOMVE010000004 DAOMVE010000000
VERSION     DAOMVE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 29889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29889
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_217_length_29889_cov_3.310149"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLS64_00470"
     CDS             <1..213
                     /locus_tag="PLS64_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRL42339.1"
                     /translation="YNMKIIKTKVDNSNGFGDGTLKAVKEAQRAGKVTVDGIVGEKTI
                     NAIYHLINDGIRAKDNKIANAKKALG"
     assembly_gap    279..288
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(429..515)
                     /locus_tag="PLS64_00475"
     tRNA            complement(429..515)
                     /locus_tag="PLS64_00475"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(479..481),aa:Leu,seq:cag)
     gene            complement(751..2238)
                     /locus_tag="PLS64_00480"
     CDS             complement(751..2238)
                     /locus_tag="PLS64_00480"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HRL42340.1"
                     /translation="MAKATGARVVVVNDSRIYGKAKPHRVYSENEYAELDATSPSSLA
                     GQLMRTRETTLHSVLKNSESTVTTLRTGIILGASSNFTSVLDPVFDDIANRRDTVVPA
                     TRDRCTFVYINDVLKAIVFAMTNLEENAVYNVGGKNCNASLIMIAAVLNDIYGSRCTI
                     ESGDFTELDGCAINSNKISVNECTPDIDLETMLKICIMDKMKSEKVLRIPHSHERRLD
                     SIHEIQLAFLLETDRICRKHNIKYFLGGGTLLGAIRHKGFIPWDDDADIMMLREDFDR
                     FCEIAPKELPSNMTFQSYHTDKACFYEFAKVRLDDTFFATDFAKDHHAMHNGIAFDIF
                     CHDNTANSAIGRKIHMAVTLFTRALVFNKWNNRKAENGSRIQSIVTNFCKKIFPLRFS
                     MWLEVRTLKFFKNKKNAKYLYDGMGRNIYNGAFPKEYLDDVAYADFEGYKFPVPKEYD
                     KYLTFLYGDYMELAPLSTRMGCHEIALCDIGKYDGFKIRKPDSEK"
     assembly_gap    2438..2447
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2448..2746)
                     /locus_tag="PLS64_00485"
     CDS             complement(<2448..2746)
                     /locus_tag="PLS64_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42341.1"
                     /translation="MRIKDFLNEFEADRAALPGVEKETLAKLRNKTIVISGGELARCL
                     CYAFLYNNEAKRLGIKVILLGKSRNAMASYHSELLLRDDFDFVDYNSALEISSAD"
     gene            complement(2746..3840)
                     /locus_tag="PLS64_00490"
     CDS             complement(2746..3840)
                     /locus_tag="PLS64_00490"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL42342.1"
                     /translation="MDCTVKGNLPSEIFEDIKKIADNRINFQQLKNKTVFVSDCHGLI
                     AYYIICTLLEGNDFFENNTRVITLAKSREDAEKQFGSLTLRKDFVVEIGESKNFPEIE
                     RADFVIYCNCPCEVAEEDCSNPEIADTITSGFANVLEYAKESNAESVLLVSSYMVYGE
                     VFSGKNNICENDLGYLDPTDADSAYAQSMRSAETLAVCYAEKFGMNVKIARPCPTLGG
                     VRMSDERKWAKLIVSAAKNQSIMLTDNGGEKFSFCYVTDTVSALIDILLNGKSGEAYN
                     ISNDNANVTMREFAQLVKSANPEKNLSVVFVHRKDEEEPEFSPSSPTPYVLCNDKIKS
                     LGFSPKTTLKDGIKRSIRATELRAELRRIK"
     gene            complement(3912..4658)
                     /locus_tag="PLS64_00495"
     CDS             complement(3912..4658)
                     /locus_tag="PLS64_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42343.1"
                     /translation="MAETKVSDPIITFKNVSKTYTLYKDDKARFKALFFKPRNAKTNK
                     ALNNVSMVINRGESVGIVGDNGAGKSTLLKMITGVAFPDEGEITVDGKVAALLELTAG
                     FSMEMTGRENIYLKGYVLGLEDSYIKQIEEKIIDFAELGDYIDQPVRTYSSGMKMRLG
                     FAINVNIEPDVLVVDEALSVGDATFKKKCKNKIKEIIESGVTVLYVSHNAASVKEICA
                     RSIFLKKGTVMFDGPTDETLRVYEESKKKK"
     gene            complement(4671..5492)
                     /locus_tag="PLS64_00500"
     CDS             complement(4671..5492)
                     /locus_tag="PLS64_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL42344.1"
                     /translation="MFTILKEHRGFGKQILLLAKNELIKTYKGAVIGPLWAVVKPAFT
                     IFVYWFAFSCGIKQLKTLHLDIGGAQPFSIDFFTFMFIGIIPWFFIQDSITQGAKELR
                     INRQFITKVKFPVSTILTYTEVSRLFVHLILMAFMYAYVLFTIGPSWYNLQFFLYCPL
                     MFIFFLALTWSTAPMSAFSKDFESLIVSVMSGIFWLSGIMYNSYGFDSPFLNKLMLIN
                     PINFFVNGYRNCFLYNRPFYANKTECLVFAIEFVVIFALGIFNYNRLRKKLPDVL"
     gene            complement(5869..6957)
                     /locus_tag="PLS64_00505"
     CDS             complement(5869..6957)
                     /locus_tag="PLS64_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HRL42345.1"
                     /translation="MKRIISIVLASAMTATCAACLSGCGGGASADSADAGEVNVYNWG
                     EYISNGEDDSLDIIEEFEKRTNIKVNYTTYETNEELYNMLKNSNVSYDVVIPSEYMIS
                     RLIDEDMLLELNFDNIPNYDNLMDRFKKLACDPEGKYTVCYSWGVTGMVYDKTKVKTK
                     PDSWDALWNKDLSGQILMFNNSRDAMAIAMQLEGINPANCTKADVDKAAAKLKEQKPL
                     LKKYVMDQVFTEMENSQSAIAPYYAGDIMTMIDNNEDLDYAMPKDGSNLFYDAMCIPK
                     CSKNKENAEKFINFMQDPEIAAANFEYLNYATPNQVAYDDYIDEDAKKNEFIFPSDEY
                     LDKCYVFSNVSDEVYSYMQEQFVKIQAD"
     gene            complement(6990..7796)
                     /locus_tag="PLS64_00510"
     CDS             complement(6990..7796)
                     /locus_tag="PLS64_00510"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL42346.1"
                     /translation="MKKKTGILSKIYIAILLIFLYSPIAVLIFYSFNNGKTTVWKGFT
                     FKWYIQLFRDDNIMNALWNTVIIAVLASVFATILGTAAAIGISNFKGKKRMLIQNASN
                     IPIISPDIVMGVSLMLLFTTLGVIFNFEMGFFTVLISHICFCVPFVVLNVMPRIKRMD
                     QSIYDAALDLGCNQWQAFYKVIIHELMPGIFSGLLMSFTYSLDDFIITYFTRGAKFQN
                     LSIEIYSMTHRRISPKINALSALLFLAVLIVMVIINLRDRHEEKVQKKRT"
     gene            complement(7796..8587)
                     /locus_tag="PLS64_00515"
     CDS             complement(7796..8587)
                     /locus_tag="PLS64_00515"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL42347.1"
                     /translation="MKSRKPSIPYIIWMVVFTMIPIIMIGFTAFTDKNGNFSLQAFTN
                     AFYYTNIFVKSLWIALISTAICLVLAYPLAYLLTRMKESTQNTLIMLLMIPMWMNFLL
                     RIYAWVTLLQDNGPIDMMLSLLGIHSTYIGNTGAVILGMVYEYLPFMVLPIYTVMSKI
                     DNGLIEAAQDLGSNNFTVFRKVVAPLSVPGIISGVTMVFVPSASTFLVAQYLGGPDDI
                     MIGDVIEKIFWTDQNTGSAISLILMIFIFVFLIIMNLFGDEEAIA"
     gene            complement(8972..10015)
                     /locus_tag="PLS64_00520"
                     /pseudo
     CDS             complement(8972..10015)
                     /locus_tag="PLS64_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377041.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            10573..13743
                     /locus_tag="PLS64_00525"
     CDS             10573..13743
                     /locus_tag="PLS64_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL42348.1"
                     /translation="MKKAKKIASLVVASALLTSSFAAITSVNAAEVDSAQTASADSTL
                     RDKVGDGVMLHAFNWSYNTIKENLPAIAAAGYTTVQTSPVQQPKDYSTSGDVTGQWWK
                     LYQPISFHIAEESWLGTKDDLKSLCDEADKYGIKIICDIVSNHIANADEARPETVSNQ
                     VKKYEPEFYKKRKTYTRTYKGDANDSSVQAVVQGHVSKCPDLVTNDTAVQGYIINLLK
                     ECIDCGVDGFRFDAAKHIETEDDGEYASDYWKNITTSASSYYTQKTGDDLYIYGEILN
                     NCGADRSYSSYTKYINVTDNRTGDAVLYNVTRGKASTATNAKYKSGVAASNAVLWAES
                     HDTYEGSSGSSGFSNTADVSDENVVKAWAIVASRKDSTALFFARPGTALMGNISTDST
                     YKSTAVSEINKFHNLFVGQSEKLGSSGDIAYVARGTSGIVLSNCKGTNASVSISGTGL
                     ADGKYTDTVSGAEFTVANGVLTGSIGNTGVAVVYNGTTTPKAINSVESGSFRGDTMTL
                     TLSLENATSGTYCLDDSTPVKFTGTTSIRIGSDYKPGETINLTVTATDGVKTSSMVYK
                     YAKSTAQESGVYVFFNPANKKGWSAPYQVYIFDETTNKGTVYKNANWPGEAMTLDPAT
                     GYYYYEVPKSSSISADEDDENQAASDFDLSTSANTRVIISEKGGEQYPGRTGTPISLN
                     GSSKAFSLTKAGTWEDTTLTPTKVEIEATDVTKGSAAEPTTKPVPSTQPTTAQPATEP
                     SGNAVYGDVNGDGDITVVDATLVQKHVVQLETLSADKQILADVNGDNTISVVDATLIQ
                     KYIVQLKDWGRTGDVYQAEQPTTPEPTTAEPTTVKPTAQPTTKPADTYTLYFKTKLGW
                     MTSDGVSLFAYDLDTGASYQFEQDTDAYPNVYTVEVPATVSNVSVYRSLSEVDEKPVG
                     GDDGNVYNCWDATVSKTNNCITLSNDEAVSTAPYVPEQKPAFTLSRVYFDNSKAHYTD
                     VYIYGWAESGLGNAAAHMNKIAGTDIWYYDFDTPLSPGSNCFLFKDTESTWKTQTNDL
                     TVADGKNCFRANPGSKSGGVWVSYSE"
     gene            13918..14487
                     /locus_tag="PLS64_00530"
     CDS             13918..14487
                     /locus_tag="PLS64_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42349.1"
                     /translation="MKTEQIFQEFEQRLEKEYYAQLASKEILTVPLLIEIFLDDDYAN
                     AHWAEQVLEYICEVNPKLVYPFFEFVAKGLDKCNGFLAWNTWKMITKLLPTDTENKFE
                     SVKERFYDALASKTPAEFSIACDCAVSVFINKADEQKKLLDILKKSAEHKFYIGDTEI
                     ENSGEIAKEKVQLFLERIMNYKTKAENNC"
     gene            complement(14696..15712)
                     /locus_tag="PLS64_00535"
     CDS             complement(14696..15712)
                     /locus_tag="PLS64_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRL42350.1"
                     /translation="MDIAVDLGSDRTRVFIENKGIVLDEASVVAFDVDTYDIVGVGNE
                     AYKMIGKTPAGIRAMHPIDDGVISRSELVEDMVSILIKEVSPSKVTMPRVVASIPSEV
                     TEVEKRAVVNAVSSIGVRKVYLIEAPKAAAMGCSLDITSPHGILIADVGSGTADIAVL
                     SLGGISVSKCIKSAGCAMDEEIVKYIRKEHNLIIGTNMAEACKKAVGCVKIPKEPKLF
                     RVKGRDAVSGLPKFIDVGYAEIKPVIEDIALNIIRAIKDVLEQTPPELVGDIYTDGII
                     LTGGLAKLTGFARLLSESTKLKVRVHKAAADCVINGCGKAIEYIDHPEKIQPGAVNPL
                     IEAF"
     gene            complement(15729..16391)
                     /locus_tag="PLS64_00540"
     CDS             complement(15729..16391)
                     /locus_tag="PLS64_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRL42351.1"
                     /translation="MTHFIKKVFGTIKGFFFTVRCPYCGRVIEPNKSCCDKCRKEFPE
                     MPLVRYATGGYICTSPFPYDGIFRRAVLNFKFHNCGAYAELLSHEMVRSIKDVYRDRE
                     FDLVTCVPMHKDTLRERGYNQAELLAKECARLLGIEYADLLAKTKKNKTQHSIKAVER
                     AKNVIGVYSPIDKNLIKGKRILIIDDIITTGSTLGECARILNKCKCGEICCCTLCTSL
                     LI"
     gene            complement(16375..18588)
                     /locus_tag="PLS64_00545"
     CDS             complement(16375..18588)
                     /locus_tag="PLS64_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HRL42352.1"
                     /translation="MNNDNLLQLEGAIESVVFRNDENGYTVIELADGDDYLTAVGIMP
                     QVSSGDVVKLTGKYTNHPSYGRQFAVQICEISRPTEAADILRYLSSGAIKGIGTQTAQ
                     RLVKEFGEATLDVLENQPERVAMLKGISSQKAEDFSKQLKQNTGVRTLMLFLGEYGIS
                     NTASVKIFNAYGPSCVDLIKKNPYILCQGDFGVSFESADFIAKKEKLEPDSNVRMRAG
                     IVYILHHNERNGHTCLPKEKLLKVSTDFLGIDGEKVSECMEEMLFDRSLIEDKIDGKK
                     FVFPPNVHLCEMYIASRIKMLLKFPSEKIKNIDKAIEKCEKEDGIEYADLQKKAITMA
                     LTEGMLVLTGGPGTGKTTTLNAIIKIMKANGKTVLLSAPTGRAAQRMSELTGDEAKTL
                     HRLLEVSWDKHDNPVFNKNERNQLKCDALIVDEVSMVDAFIMESVMRALSTGCRLILV
                     GDSDQLPSVGPGNVLGDLIESGLIPVVRLNEIFRQAQQSLIVTNAHKIVNGEIPILNS
                     ADKDFFFLQRNNKSEVSAVIADLCANRLPKAYGYSPFENIQVLAPSKKGELGTAELNH
                     KLQAALNPKDDDKAEITIGSKTFRTGDKVMQIKNNYDIRWFRENGETGEGIFNGDIGI
                     ITKIDRKTKSLVVKFFDKIARYTFEFAGDLDFAYAITVHKSQGNEFDAVIIPMFSGPP
                     QLYYRNLLYTAVTRAKKTLVLVGNPSTVEYMVNNNRRTKRYSGLKEFLLRDDTLY"
     gene            complement(18595..19317)
                     /locus_tag="PLS64_00550"
     CDS             complement(18595..19317)
                     /locus_tag="PLS64_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HRL42353.1"
                     /translation="MKILHLYHDIMNLYGEYANVSALERMLEKSGVKFTTDRLTLFDN
                     ADLGDYDFIYIGSGTEKNQKLVLQDFKKYKDFLVEYINSGKVILMTGNSFEMLGKTIT
                     DSDGKVFDGLGIYDFTVTEQNKRRITGDIVYTSDILTKPIVGFVNKCSEIKGIENHLF
                     TVKHGLGDYENAKAEGLKDKNLFATHVTGPIMIKNPHFLEYIALQISKNTDGFKPCTD
                     YLDYERAGYEITLGELTKRFEE"
     gene            complement(19320..20645)
                     /locus_tag="PLS64_00555"
     CDS             complement(19320..20645)
                     /locus_tag="PLS64_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HRL42354.1"
                     /translation="MKKLFTIIVCKVLKFVGGLLGKGSSLPGQIALKLCPDILGRIEL
                     PKYIVAVTGSNGKTSTVEMIAHILCESGKKVAWNKEGSNQIEGVTTLILGSCTLSGKV
                     KSDILLIESDERFAKYTFKYITPTHYVITNLYRDQLTRNGHPEWVYNAVKDSIHPETQ
                     LILNADDPLVSCFGYGRDNVVWFGAGNLPTDTKEFVGVYNDGAYCPHCKSPMTYSSYH
                     YDHIGNYECPNCGLKRQDTSYTVTSADLEKGEITINNKYKIELTLKSLYNVYNLLAAF
                     TVASITGVDGNTIAKSLSNYVLKNFRVVTFTLGNRKGTLVTSKHENSISYNQSLKLAA
                     SDKDKCDVLIIVDAVSRKYFTSDVSWLWDINFDILKSDNVKNIVLAGTYCNDLATRFS
                     FSKVDRNKIKVIKDIEEAVDYLDNDRKEKIYVITCFSDKGKFLEKVKVD"
     gene            complement(20883..21077)
                     /locus_tag="PLS64_00560"
     CDS             complement(20883..21077)
                     /locus_tag="PLS64_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42355.1"
                     /translation="MEEKRKTVTSTEVKRRYNQKVYSQISFSAPKELVEEFRGICRNI
                     GISQASVFKKFIADFVEKYR"
     gene            21334..21933
                     /locus_tag="PLS64_00565"
     CDS             21334..21933
                     /locus_tag="PLS64_00565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRL42356.1"
                     /translation="MTEKEKMLNSQMYNPMGDKQLRNERCSVKNLCREYNNLPFEDEE
                     TQKILIKKIVGSTKENFYITAPFWCDYGYNIELGENFYSNHNMVILDCAKVKFGDNVF
                     VAPNCGFYTAGHPVDYPRRNAGFEYAYPITVGDNVWIGGGVQVMPGVTIGSNVVIGGG
                     SVVVKDIPSDCVAVGNPCKPIRKITDEDMKTCFDKSMNK"
     gene            21951..23201
                     /gene="hflX"
                     /locus_tag="PLS64_00570"
     CDS             21951..23201
                     /gene="hflX"
                     /locus_tag="PLS64_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRL42357.1"
                     /translation="MELKSNEEKITRALLVSVDTGEYDAQASLDELFELVKSAGADPV
                     LSVTQNLQKIEKGTFVGTGKLAEIAEICESQEIDLLVFDSELSPTQIKNIEAETDVRV
                     IDRTTLILDIFAQRARSKEGKLQVELAQLKYMLPRLTGKGIAMSRLGGGIGTRGPGET
                     KLETDRRHIRRRIESLKEELSDLEKHRQMLRSRRKKDGVITCAIVGYTNAGKSTLMNC
                     LTDAGVLAQDKLFATLDPTSRALKLPSGVTVMMIDTVGLVRRLPHHLVEAFRSTLEEA
                     AQSDIILNVCDASSDEARTHMQVTTDLLESLGCGDTPIITVLNKCDLLDETMLAQDFK
                     ACVRISAKNGTGIDELLNAIENNLPVRMKRVKILLPFAQAGLANEIRNKGTLIYEEYV
                     AEGLSVEAVVDEALYAKLAKYECE"
     gene            23270..23875
                     /locus_tag="PLS64_00575"
     CDS             23270..23875
                     /locus_tag="PLS64_00575"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL42358.1"
                     /translation="MSFFDMIQSFDTSVLEFIQNTFKCSFLDPIMAFFSYMGEMGLFW
                     IAVGIVFIIFKKTRSMGVMMLVAMAIGFLVGEIGIKNLINRPRPFMVNIDYSARPSEL
                     NPDFGLNIAIPSGSSFPSGHSCSSLAAATVMLIKDKRFGIPALVLALLIVFSRLYNYV
                     HYPSDVLCGMLLGVICAVVVVLVFKKTGLDNRLSPQKVKEG"
     gene            23880..24101
                     /locus_tag="PLS64_00580"
     CDS             23880..24101
                     /locus_tag="PLS64_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42359.1"
                     /translation="MKNYRFVGKTSKETFLIKGINVFNERWIHEGSCATVTNPQNGKS
                     YVFSKYISGGVEFVAGKDASGYWLFFVEV"
     gene            24153..24827
                     /locus_tag="PLS64_00585"
     CDS             24153..24827
                     /locus_tag="PLS64_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HRL42360.1"
                     /translation="MENEKLLAKNLFDYSRTIAKPLLETVDYPWEALPKISEFIIELG
                     KTLDPEIYEQRGENVWVAKSAKVFDSAYLGGPLIICEDAEIRQCAFIRGNAIVGRGAV
                     VGNSTELKNSILFDGVQTPHYNYIGDSILGYKSHTGAGVITSNLKSDKSLVTVLCDGD
                     KVDTNVKKFGAMLGDHVEVGCNSVLNPGSVIGRNTNIYPLSFVRGYVPENSIYKRLGE
                     VAEKIE"
     regulatory      24984..25081
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            25152..26534
                     /locus_tag="PLS64_00590"
     CDS             25152..26534
                     /locus_tag="PLS64_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL42361.1"
                     /translation="MKKYDVIIVGAGPGGIFSAYELVTNNSEIKVAVFEKGHLLEKRK
                     CPIDGDKVKNCISCKTCSIMSGFGGAGAFSDGKYNITNDFGGTLYEHIGKKKAIELME
                     YVDKINMKFGGEGTKLYSTAGTSFKKLCLQNKLNLLDASVRHLGTDINYIVLKNLYDY
                     LENKVDFYFDTPVENVELKNGNDKEGYLVDTADENYECKKCIVSVGRSGSKWMEHVCH
                     NLNIPTKSNRVDIGVRVELPAAIFSHLTDELYESKIVYRTEQFEDNVRTFCMNPKGCV
                     VNENTNGIITVNGHSYEDKAKQTENTNFALLVSKHFSEPFKDSNGYGESIARLSNMLG
                     GGVIVQRFGDLIRGRRSNPSRIKEGLVVPTLDATPGDLSLVLPKRILDGIIEMIYALD
                     KIAPGTANDDTLLYGVEVKFYNMEVDVDEHLESPHKGLYIIGDGSGVTHSLSHASAGG
                     VFVAREILAN"
     gene            26975..27073
                     /gene="ffs"
                     /locus_tag="PLS64_00595"
     ncRNA           26975..27073
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLS64_00595"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            27142..28896
                     /gene="dnaX"
                     /locus_tag="PLS64_00600"
     CDS             27142..28896
                     /gene="dnaX"
                     /locus_tag="PLS64_00600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRL42362.1"
                     /translation="MYQVLYRKWRPKTFADVSGQEHITTTLLNEISTNRLNHAYLFTG
                     SRGTGKTTCAKILAKAVNCLHPVNGNPCGECEICKGIDDGSILDIVEMDAASNRKIDD
                     IRQIIDEVQFKPAKCKYRVYIIDEVHMLTTEAFNALLKTLEEPPEHVIFILATTEVHK
                     LPQTILSRCQRFDFHRIPPRAIADRLLYVANQEGVTLSDSAAMLAASVADGALRDALS
                     LLDRCIAISSEIDEDVVRSAAGLARKTYLFELSNCVINKNTAKALEIVDRLYGESKDM
                     ARLCDELLSHFRTLMLIKSVKNPRDIVIMADDEFEQAQTQSDYLSLADIVYYMDVLSK
                     AYQNMGRGTGDRTELEMAVVKLSSAELDGTIEALTARVTALEKAVKKGIKVNYVPEQV
                     QTVPQSVQNETPKPEVQNSEPEKSQETKPVSEKIKDEPVIPTPPVEMPTPKQPVQPIQ
                     EQPVSAQQKPKKTAQRQSVDLDAIYNNAQPFPDWAEVVNNMKPVSRAIAAAFANSAAY
                     TSGKYLLIDTDNEMAFELLKNNERRQEIRQLILETTGQHYNLGPYKRPSAKQEEKTDP
                     VDKLIESLQGSDVIITQE"
     gene            28916..29266
                     /locus_tag="PLS64_00605"
     CDS             28916..29266
                     /locus_tag="PLS64_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRL42363.1"
                     /translation="MKARLPKGYGSGGANNIQQLARQAQKLQDDMEAASKELETKEYE
                     ATAGGGAVKVTVTGKMELTKVEISPEVVDPEDVEMLSDLVMAAANEAIRAAAKEKEDK
                     METISGGLNIPGMF"
     gene            29269..29871
                     /gene="recR"
                     /locus_tag="PLS64_00610"
     CDS             29269..29871
                     /gene="recR"
                     /locus_tag="PLS64_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRL42364.1"
                     /translation="MSGYNVAPLENLVEQFERMPGIGNKTAQRLAYYVLNLSKTEAEQ
                     LAGAVMDAHTKIHYCSKCCNLTDKDLCPVCANPARDHSVICVVETPRDATAIENTHEF
                     KGVYHVLHGSISPLNDIGPDNLTIKELLARLNEESVEEVIMATNPTVEGDATALYISK
                     LLKPLGVKVTRLAYGIPVGGDLEYADEYTLAKALEGRNEI"
BASE COUNT         8666 a         6262 c         6340 g         8601 t
ORIGIN      
        1 tataatatga aaatcatcaa gactaaggtc gataattcaa acggtttcgg tgacggcact
       61 ttgaaagctg taaaagaggc acagagagca ggtaaagtca cagttgacgg tatcgttggc
      121 gagaagacca tcaatgctat ctatcatctc atcaatgacg gtattcgagc aaaagacaac
      181 aaaattgcca acgcaaaaaa ggcactcggc taattaaaac ctaaaggaca ttcttaatgt
      241 cctgacaaac acatgattgc aaaaaaaatc ccctcatcnn nnnnnnnnga ggggagtttt
      301 gtcatttgta gatttgttag ctacttgtta gctgtgtgtt agctacgata tgtatttttc
      361 cgtgttttag agtgatttaa gtatagcaaa accccagtaa atatcgtatt taccggggta
      421 aaaagctatg gtgcaggtaa caggacttga acctgcatga aattgctttc acatggacct
      481 gaaccatgcg cgtctgccaa ttccgccata cctgcttatt aaattgaaaa ttgaaaatgg
      541 aaagttgaaa atgattgtgt caacttttgc ataatcaatt taaattccct tgatttttac
      601 acggtgggga aaccgaggcg gagcttactt tcagataaat cttacttttc ggctgattcc
      661 gcaacagtac tcaaaatgtg ctatattatt atagcagacc gacaggtaag tgtcaagtca
      721 catttatttt atcggtctgt tttacgctga ttatttttca gaatcgggtt tgcggatttt
      781 gaaaccgtca tattttccga tgtcgcagag ggcaatttcg tggcaaccca ttcttgttga
      841 cagcggtgca agctccatat agtcgccgta gaggaaagta aggtacttgt catattcctt
      901 tggcacaggg aacttgtaac cctcaaagtc ggcataagca acatcgtcaa gatattcctt
      961 tggaaaagca ccgttataaa tatttctgcc cattccgtca tagagatatt ttgcattctt
     1021 tttgttttta aagaatttta aagtacggac ttcaagccac atactgaatc tgagcggaaa
     1081 tattttcttg cagaaatttg ttacaatgct ctggattcta ctgccgtttt cagccttgcg
     1141 attgttccat ttattgaaca ccagcgctct tgtgaacaga gtcacagcca tatgaatttt
     1201 tcgtccgatt gctgaattgg ctgtgttatc atgacagaag atatcaaacg caattccgtt
     1261 gtgcattgcg tgatggtctt ttgcaaagtc ggttgcaaag aaagtgtcgt caagtctgac
     1321 cttggcaaat tcatagaaac aagccttgtc cgtatggtac gattgaaaag tcatattgct
     1381 cggaagttcc tttggcgcaa tctcgcaaaa gcggtcaaaa tcttcacgca acatcataat
     1441 atcggcatca tcatcccacg gaatgaatcc tttgtgacgg attgcaccga gaagtgttcc
     1501 gccgccgaga aaatatttta tgttgtgctt tcgacaaatt ctgtcggttt caagcagaaa
     1561 tgcaagctga atttcgtgaa ttgaatcaag tctgcgctcg tgtgagtggg ggatacgcag
     1621 aactttttcg gacttcatct tgtccattat gcagattttc agcatggttt caaggtcaat
     1681 gtcgggagtg cattcgttta cggaaatctt gtttgaatta attgcacagc cgtcaagctc
     1741 cgtaaaatcg cccgactcaa ttgtacagcg actgccgtaa atatcgttga gaacagccgc
     1801 aatcataatc agcgatgcgt tgcagttttt gccgccgaca ttataaactg cgttttcttc
     1861 aagatttgtc attgcgaaaa caatcgcctt taaaacatcg ttgatataaa caaaggtgca
     1921 gcgatccctt gttgcaggaa caactgtgtc acggcggttg gctatatcgt caaaaacagg
     1981 atcaagcacg cttgtaaagt tgcttgacgc tcccaaaatt atgcccgttc tgagcgttgt
     2041 aacggttgat tcgctgttct tcaaaaccga gtgcaaggtg gtttctctcg ttctcataag
     2101 ctgacctgca agcgatgagg gagaggttgc gtcaagttct gcgtactcgt tttcggaata
     2161 aactctgtgc ggtttggctt tgccgtaaat tctgctgtcg ttcacaacga caactcttgc
     2221 gcctgtggct tttgcaattt tggcacaggc atttttatca gcttttttct tatttttaac
     2281 acttacatca ttttatgatt tttgcaaaag aaggcatttt ctttctgtct ggcagtgcct
     2341 atgaaaaatc aattttgccg atgcaggtta ttatgccgac gctcttgttt attggactga
     2401 ccaatgtcat gggcatccaa atgctcgtgc cacttggnnn nnnnnnntag tcggcacttg
     2461 aaatttccaa tgcagaatta taatcgacaa aatcaaaatc atctcttaac aagagttcgc
     2521 tgtggtatga tgccattgcg ttgcgtgatt tgccgagaag gataactttt attccgagcc
     2581 ttttagcctc gttattgtac agaaaggcat agcaaagaca ccttgcgagt tcaccgcccg
     2641 agatgacaat tgttttgttc ctgagctttg caagagtttc tttttcaact ccgggcaatg
     2701 ccgccctgtc ggcctcaaat tcgtttaaaa aatctttaat acgcattact ttatccttcg
     2761 taattctgca cgcagttctg ttgctcttat actgcgtttt attccgtctt taagcgtggt
     2821 tttcggacta aagccgagtg attttatttt atcgttgcac aaaacatacg gtgtggggga
     2881 tgacggagaa aattcgggtt cttcttcgtc ttttctgtga acgaacacta cagagagatt
     2941 tttttcggga tttgccgatt ttacaagctg tgcaaattcc cgcattgtca catttgcgtt
     3001 atcgtttgaa atgttgtatg cctcaccgct ttttccgtta agcaaaatat caatcaatgc
     3061 cgaaaccgtg tccgtcacat agcaaaagct gaacttttcg ccaccgttat ctgtaagcat
     3121 aatgctctga ttttttgccg cactgacaat cagctttgcc cattttcttt catcgctcat
     3181 tctgacacct ccgagtgtgg ggcagggacg ggcgattttt acattcatac cgaacttttc
     3241 agcatagcaa acagcaagtg tttcggctga acgcatactt tgtgcgtatg cactgtcggc
     3301 atcggtcggg tcgagatagc cgaggtcgtt ttcgcaaatg ttatttttac cgctgaaaac
     3361 ctccccgtaa accatatatg aagatacgag cagaaccgat tcggcatttg actcttttgc
     3421 atattcaagc acatttgcaa agcccgaagt gattgtatcc gcaatttcgg gattactgca
     3481 atcttcctct gcaacctcac acgggcagtt gcagtatatt acaaagtcgg ctctttcaat
     3541 ctccggaaag ttctttgact ctccgatttc aacaacaaaa tctttgcgga gagtaaggct
     3601 gccgaactgc ttttcggcat cttcacggct ttttgcaagg gtgattaccc ttgtgttgtt
     3661 ttcaaaaaaa tcattgccct caagcaatgt gcatataatg taatatgcaa taagtccgtg
     3721 acagtccgac acgaaaacag tcttgttttt taactgttga aaatttatcc tgttgtctgc
     3781 aattttttta atgtcctcaa aaatttcgga gggcagattg ccttttacgg tacagtccat
     3841 atgggtttac ctctcagata aatcagaaaa gctccgcaat tgtggagctt ttgctttatt
     3901 ccgaaacgga attatttttt cttttttgat tcctcataca ctctgagggt ttcatctgtc
     3961 gggccgtcaa acataacagt tccttttttc aggaagattg aacgcgcgca gatttcttta
     4021 accgaagcgg cattgtgact tacataaagt acggttacac cgctttcgat aatctcttta
     4081 attttatttt tacacttctt tttgaaggtt gcgtcaccga ccgagagcgc ctcatcaaca
     4141 acaagaacat cgggttcaat gttgacattg atagcaaaac caagacgcat tttcataccg
     4201 cttgagtatg ttcttacagg ctggtcaatg taatctccaa gctctgcaaa gtcgatgatt
     4261 ttctcctcga tctgcttaat gtagctgtcc tcaagaccga gaacataacc cttcagataa
     4321 atgttttccc tgcctgtcat ttccatggaa aagcctgcag tgagttcaag caatgcggca
     4381 accttgccgt caacggtgat ttcgccttcg tcgggaaatg caacgcccgt aatcatcttg
     4441 agcagagttg acttgcctgc accgttatcg ccgacaatac caaccgattc gccacgattg
     4501 attaccattg aaacattgtt aagtgcctta tttgtttttg catttctcgg cttaaagaaa
     4561 agagccttga atcttgcctt gtcatctttg tagagtgtgt aggttttgct gacattttta
     4621 aaggtaataa tcggatcgga tacttttgtt tcagccataa tattacctcc ttaaagtaca
     4681 tcaggaagtt ttttcctgag tctgttgtag ttgaaaatac caagggcgaa aattacaaca
     4741 aattcaattg caaacacaag acattccgtt ttgtttgcat agaacggtct gttgtagagg
     4801 aaacagttgc ggtagccgtt tacgaaaaag ttaatcggat tgataagcat gagcttgttg
     4861 agaaacggcg aatcaaagcc gtaggagttg tacataatac ccgataacca gaaaatacct
     4921 gacataacgg aaacaatcag gctttcaaag tccttactga acgcagacat cggggctgtt
     4981 gaccatgtga gcgcaaggaa gaagataaac atcaacggac agtacaggaa aaattgcaga
     5041 ttgtaccagc tcggtccgat tgtgaaaagt acatatgcgt acataaatgc cataagaatc
     5101 aggtgaacaa aaagccttga aacctctgta taagtaagga ttgttgaaac ggggaactta
     5161 acctttgtaa taaattgacg gttgattctc agttcctttg caccctgcgt tatgctgtcc
     5221 tgaataaaga accacggaat gataccgatg aacataaaag taaagaagtc aatggaaaaa
     5281 ggctgtgctc cgccgatgtc aaggtgaagt gttttaagct gtttgatacc gcaggaaaaa
     5341 gcgaaccagt aaacaaaaat tgtaaacgca ggcttaacaa cagcccaaag aggaccgatt
     5401 acggcgcctt tgtaagtttt aataagttcg tttttggcaa ggagcaaaat ctgctttccg
     5461 aagccacgat gttcttttaa aattgtaaac aattattata ccatcctatc tcttttgtga
     5521 tgcacaggcg cccgcctgca agtgaataaa atcgaaaaat gtattatgca ctctttaaca
     5581 gtatatatta tattactttt tcctaatttg tcaatcaaaa caaggcaatt gaaatctatt
     5641 tgtaagattg agcgaatccc cgttaattct gtgtattttg ggtgttaggg gtacgaatat
     5701 gacaacctgt cacggaaacc ttttaaaata aggaataata cttttaagat aacataaaat
     5761 ttaaaggctg tataaatggg ggatgattaa tttttttaaa aatttaccaa aattaaaatc
     5821 ccgaacagag cctttaaaag ctttgctcgg gattaatttt attgcttatc agtctgcctg
     5881 gatttttaca aactgttcct gcatatagga ataaacctca tcggaaacat ttgaaaatac
     5941 atagcatttg tcaagatact cgtcactcgg gaagatgaat tcgtttttct ttgcgtcttc
     6001 atcaatgtaa tcgtcatatg ccacctgatt gggtgttgca tagttgagat attcaaagtt
     6061 tgcggcggca atttcaggat cctgcataaa gttgatgaac ttctctgcat tttccttgtt
     6121 tttactgcat ttcggaatac acattgcgtc atagaaaagg tttgagccgt cctttggcat
     6181 tgcgtagtca agatcctcgt tgttatcaat cattgtcata atgtcgcctg cgtagtacgg
     6241 agcaattgcc gactggctgt tttccatttc agtaaatacc tgatccatga catatttttt
     6301 aaggagaggc ttctgctctt taagtttagc ggcggctttg tcaacatctg ccttggtgca
     6361 gttagcagga ttaatgccct caagctgcat tgcaattgcc attgcgtcac ggctgttgtt
     6421 aaacataaga atctgacctg aaaggtcttt gttccaaagt gcgtcccatg aatcgggctt
     6481 tgttttaacc tttgttttgt cataaaccat acctgttacg ccccagctgt aacatacggt
     6541 atatttacct tcgggatcgc aggcaagctt tttgaaacga tccataagat tgtcgtagtt
     6601 aggaatattg tcaaagttaa gctccaacag catatcctca tcaatcaatc tgctgatcat
     6661 atattcggac ggaatgacaa catcatagct tacattactg tttttcagca tattgtaaag
     6721 ctcttcgttg gtttcatagg ttgtgtagtt taccttgatg ttggtgcgtt tttcaaactc
     6781 ctcgataatg tcgagtgagt catcttcgcc gtttgaaatg tactcgcccc agttgtaaac
     6841 attaacttct cctgcgtcag ctgagtcagc cgaagcacct ccgccgcagc ctgaaaggca
     6901 agccgcacat gttgccgtca ttgcagatgc aaggacaatg ctgatgattc ttttcatttc
     6961 tcaatctccc tttaataaaa tttttaaaat caagttctct ttttctgcac cttttcttcg
     7021 tgtctgtcac gcagattaat aataaccata acaatgagta cagcaaggaa aagcagtgcg
     7081 gaaagagcgt tgattttcgg tgaaattctc ctgtgagtca tcgagtaaat ttcaatagag
     7141 aggttctgga attttgcacc tcttgtgaaa tatgttatga tgaaatcgtc aagagaatat
     7201 gtaaaactca tgagcagacc cgagaaaata cccggcataa gctcgtgaat aattaccttg
     7261 taaaatgcct gccattggtt acagccaagg tcaagggcgg cgtcgtaaat gctctgatcc
     7321 attcgtttga ttctcggcat aacattaagc acaacaaacg gaacacaaaa gcatatgtgc
     7381 gaaattaaaa ccgtgaaaaa tcccatttca aagttaaaga taactccgag agtcgtaaac
     7441 aaaagcatga gagaaacacc cattacaatg tcagggctga tgatcggaat gtttgacgca
     7501 ttctgaataa gcattctttt tttgcccttg aaattgctga tgccgattgc ggcggcagtt
     7561 ccgaggattg ttgcaaaaac agatgcaaga acggcaataa taacagtgtt ccacaatgcg
     7621 ttcataatat tgtcatcacg aaaaagctgt atataccatt tgaatgtaaa tcctttccag
     7681 acggtagttt ttccgttgtt gaaggaatag aagataagta cggcaatggg cgaataaagg
     7741 aaaataagca ggattgcaat gtaaattttt gaaagaatac ctgttttctt tttcattatg
     7801 caatcgcctc ctcatcaccg aacaagttca tgattatcag gaacacgaaa ataaaaatca
     7861 ttaatatcaa tgaaattgcc gaacccgtgt tttggtctgt ccagaatatt ttttcaatga
     7921 catcaccaat cataatgtcg tccggaccgc cgagatactg tgcaaccagg aatgtgcttg
     7981 cactcggaac aaataccatt gtcacacccg aaataattcc gggaacgctg agcggtgcga
     8041 caacctttct gaatacggta aagttgtttg aaccgagatc ctgcgccgcc tcaataagac
     8101 cgttgtcgat tttgctcata acggtgtaaa tcggcagaac cataaacgga agatattcat
     8161 acaccattcc gagaattacc gcacccgtgt tgccgatgta ggttgaatga atacccaaaa
     8221 gcgagagcat catatcaatc ggaccgttgt cctgcaaaag cgttacccat gcgtagattc
     8281 ttagcagaaa gttcatccac ataggaatca taagcaacat aatgagcgtg ttctgggttg
     8341 attctttcat tctcgtgaga aggtatgcaa gcggatatgc aagtacaaga cagattgccg
     8401 ttgaaataag cgcaatccaa agtgatttta caaaaatgtt tgtgtagtaa aacgcatttg
     8461 taaatgcctg aagcgaaaaa ttaccgttct tgtcggtaaa ggcggtaaag ccgatcataa
     8521 taatcggaat cattgtgaaa acaaccatcc agattatgta gggaatagag ggcttgcgtg
     8581 atttcattta tcagccctcc ttttccgaaa actctgagat tacctcaaac cgagcctttg
     8641 aaaagttgaa tgaaagcgga acataggttg cgacctgctc gtccgtatcg ctgagcataa
     8701 gccactttct ttcgtccgat tcaagaatga tttcgttgtg ttcacccttg taaactacgg
     8761 attcaatgta caaatccttt aaatcgccca cgccgtcgcc tgcaagcgaa agagcgtcgg
     8821 gcggcagagt gatttttagc tttgtacctt cttcatagta tttatcggga acggtaacgg
     8881 tttcgccctc aagctcaaat tcaaaggaat tgtcattctg attcgagtat gtaacggttg
     8941 tctcaataac attatcgtaa aacggaagaa gcttgttcat aatctgaatg ttgaacggga
     9001 taaccctcat accgattttg cttccgattt cagccgattc ggtggagtgg ataagaattt
     9061 cacactttcc gcatcttacg ctcatctcat agtgaacacc cttgaaggtt gtgttctcaa
     9121 cagttccgac aagctgaccg tcctcgggag aggtaacttc aatatcctcg ggacggatga
     9181 caacatcaac aggagtattc ctgccgaatc ccttgtcaac acattcaagc tctttgccga
     9241 gaatttgaat tctgcagtca tcaagcataa ttccgttgag aatatttgcc tcaccgataa
     9301 agtctgcaac aaatgcgttc acaggctcgt tgtatatatc ctcgggagaa ccaatctgct
     9361 cgattacacc gttgttcata acaacaacac ggtcgctcat ggtcagagcc tcttcctgat
     9421 cgtgagtaac atatataaat gttttctgag ttttctgttg gatttcctta agctcaagct
     9481 gcatactctt acgcagttta aggtcaagag caccgagagg ctcgtcaagc agaatgattt
     9541 cgggatcgca cacaagcgct cttgcaattg caacacgctg ttgctgaccg ccagaaagct
     9601 ttgcaacgga acgcttttca taacccttga ggtcaacggt tgcaagcata tcggttacac
     9661 gcttttttat ttcatctttc ggcatctttt taagcttgag tccgaaagcc acattatcaa
     9721 aaacattgag gtggggaaac aaagaatatt tctggaatac cgtgtttaca tttcttttat
     9781 tcggaggagt tccgttaatt cttttgccgc cgaaaatgac atcgccgctg tcgggttcaa
     9841 caaaaccgcc gatgatgcgc agggttgtgg ttttaccgca gcctgacgga ccgagaatag
     9901 ttacaaattc cttttcattt atatcaaggt tgatgtggtt aagaattacc tcaccgtcaa
     9961 atttgatgaa aatatccttg agtgaaacta ttgttttctt tgatgtctgt tccatttatg
    10021 cacccctgtt ccgttgtgat aacagtaaaa tccgtaaaaa ataaagcgga ttgtgtcagc
    10081 ctgaaaaatg caggcgaaca cagtccgctc tgccgtttct gcaaagacat aaccatgatg
    10141 ctcagaattt ttgaacagaa aaataagacg gctgtgtcgc cccgtccttt ttctttacac
    10201 aaaatcctta ttaaccgctt aaaagcgatt gcacgcaata caatttaaat tttccgaaat
    10261 gtcacccgtc acataagcat caggggacaa ctgctctttg attatattct aaatgcacaa
    10321 aaatgtcaat caatatcgtg taaaaagaaa atcaaatttg catcactaat attcttttaa
    10381 agaagtaaag ttcatacaga gtcagaaaag gttaaatata atgctttttg gtgcaaaatt
    10441 attgttgaaa aatttttaca accgtgtaat ctgcacaaaa aacagccctc gaatttgtaa
    10501 cataaagtat gtactttttt ggtgaaattt gttataatga aatcgagaat tttatgaaag
    10561 gatgttttcc ctatgaagaa agcaaaaaag attgcaagtc ttgttgttgc gtctgcgctt
    10621 ctgacatctt cttttgcggc aattacctcc gtaaatgctg ccgaggttga ttccgcacag
    10681 actgcatctg ccgacagtac ccttcgtgat aaggtcggcg acggtgttat gctccacgca
    10741 ttcaactggt cttacaatac aatcaaagaa aaccttcccg caattgcagc ggcaggatac
    10801 acaacagttc agacttctcc cgttcagcag ccaaaggact actcaacctc aggcgatgta
    10861 acaggtcagt ggtggaagct ttatcagccg atcagcttcc atattgcaga ggagtcatgg
    10921 ctcggcacaa aggatgatct taaatcactt tgtgatgagg ctgacaaata cggcatcaaa
    10981 atcatctgcg atatcgtttc aaaccatatt gcaaatgccg atgaagctag gccggagact
    11041 gtgagcaatc aggttaaaaa atatgagcct gagttctata agaaaagaaa aacctacacc
    11101 cgtacgtaca aaggcgatgc aaacgacagt tcggttcagg cagttgttca gggtcatgtt
    11161 tcaaagtgtc ctgaccttgt aacaaacgat actgctgttc agggatacat tatcaacctc
    11221 ctcaaggaat gcatcgactg cggtgttgac ggtttccgtt ttgatgccgc aaagcatatt
    11281 gaaacagagg atgacggcga gtatgcgtct gattactgga aaaatataac aacctcggct
    11341 tcttcttact acacacagaa aacaggcgat gacctctaca tctacggtga aattcttaac
    11401 aactgcggtg cagacagaag ctactcctca tacaccaagt atatcaatgt tactgacaac
    11461 agaacgggcg acgcagtgct ttacaatgtc acaaggggta aggcaagcac agctaccaat
    11521 gcgaaataca aatcaggtgt tgcagcgtca aatgcagttc tctgggcaga aagccacgat
    11581 acatacgagg gcagctcggg ttcatcggga ttctcaaaca ctgccgatgt ttcggatgaa
    11641 aatgttgtaa aggcatgggc aatcgttgca tcaagaaaag attcaacagc acttttcttt
    11701 gcccgtccgg gaacagctct catgggtaac atttcaacgg attcaactta caagagcaca
    11761 gctgtttccg aaattaacaa gttccacaac ctttttgtag gtcagtcgga aaagctcggt
    11821 tcttcaggcg acattgctta tgttgcaaga ggcacaagcg gtatcgttct ttcaaattgt
    11881 aaaggtacaa acgcaagcgt aagtatttcc ggcacaggtc ttgcagacgg caaatatacg
    11941 gatacggttt caggtgctga gtttacggtt gcaaacggcg ttctcacagg ttcaatcggc
    12001 aacacgggtg ttgctgttgt ttacaacggt acaacaaccc cgaaggcaat aaactctgtt
    12061 gaaagcggtt cgttcagggg cgacacaatg acccttacac tcagtcttga aaacgcaaca
    12121 tcgggtactt actgtcttga tgattcaact cctgttaagt ttacgggtac aacatcaatc
    12181 cgcatcggtt cagattataa gccgggcgaa acaatcaatc tcacagttac ggcaaccgac
    12241 ggtgtaaaga cttcttcaat ggtttataag tacgcaaaga gtaccgctca ggaatcggga
    12301 gtgtatgtat tcttcaaccc tgctaacaag aagggctggt cagcacctta ccaggtttac
    12361 atctttgatg aaaccacaaa caagggtact gtttacaaga atgcaaactg gccgggcgaa
    12421 gcaatgactc ttgatcctgc aacaggttac tattactacg aagttccgaa gtcatcaagt
    12481 atttcagctg atgaggatga tgaaaatcag gctgcgtcgg attttgacct ttcaacttca
    12541 gccaacacaa gagtaatcat ctctgaaaaa ggcggcgagc agtatccggg cagaacaggt
    12601 acacctatca gcctcaacgg ttcatcaaag gcattctcgc ttacaaaggc aggtacatgg
    12661 gaggatacaa ctcttacacc tacaaaggtt gagattgagg ctactgatgt tacaaagggt
    12721 tctgcagcag aacctacaac aaagcctgtt ccgtcaactc agcctacaac cgcacagccc
    12781 gcaacagagc cttcgggcaa tgctgtttac ggtgatgtaa acggtgacgg tgacattaca
    12841 gtcgttgacg ctacactcgt tcagaaacat gtcgttcagc ttgaaacact ttcggctgac
    12901 aagcagattc ttgctgatgt taatggggat aatacaatct ctgttgttga cgcaacattg
    12961 attcagaagt acattgttca gctcaaagac tggggccgca cgggtgatgt ttatcaggca
    13021 gaacagccta caacacctga gccgactacc gctgagccta caaccgtaaa gccgactgca
    13081 cagcctacaa caaagcctgc tgacacatac actctttact tcaagactaa gcttggctgg
    13141 atgacatctg acggtgtttc actctttgct tatgaccttg acacaggtgc aagctatcag
    13201 tttgagcagg atacagacgc ttacccgaat gtttacacag ttgaagttcc tgcaacagtt
    13261 tcaaatgtca gcgtttatcg ttcactttca gaggttgacg aaaagcctgt aggcggtgat
    13321 gacggtaatg tttacaattg ctgggatgca acagtttcaa agacaaacaa ctgcatcaca
    13381 ctcagcaatg acgaagctgt ttcaacagct ccgtatgttc cggagcagaa gcctgcattc
    13441 acactttcaa gagtttactt tgataactca aaggcacact atactgatgt ttatatctac
    13501 ggttgggcag agagcggact cggcaacgca gcagctcaca tgaataagat tgcaggtaca
    13561 gatatctggt actacgattt tgacacaccg ctcagccccg gttcaaactg tttcctcttt
    13621 aaggacacag aatcaacatg gaaaacacag acaaacgact tgacagttgc tgacggtaag
    13681 aactgtttcc gtgcaaatcc gggttcaaag tccggcggtg tctgggtaag ctattcagaa
    13741 taacagaact acagaacaac cttttcttaa tattctgcta acagtaaagc attccctccg
    13801 ataaaattcg gggggaatgt tacttttaat atgattttac ttgaaaaaga atgtaatata
    13861 cagtattata ttaggagtat taaaccaaaa tttaagtgaa tttatggacg ggaatttatg
    13921 aaaacagagc agatttttca ggaatttgaa caaaggcttg aaaaagaata ttatgcacag
    13981 cttgcaagca aagaaattct gactgttcct ttgcttattg agatttttct tgacgatgat
    14041 tacgcaaatg ctcactgggc ggaacaggtt cttgagtata tttgcgaagt aaatccgaaa
    14101 cttgtctatc cgttctttga atttgttgcc aaaggtcttg acaaatgcaa cggctttctt
    14161 gcgtggaata cctggaagat gattacaaag cttttgccga ctgacacgga aaataaattt
    14221 gaaagcgtaa aagaaaggtt ttacgacgca cttgcttcaa aaacaccggc agaattttcc
    14281 atagcctgtg attgtgccgt gtccgttttc attaacaaag cggatgaaca gaaaaaattg
    14341 cttgatattt tgaagaaatc cgctgaacac aagttttaca ttggcgatac tgaaattgaa
    14401 aacagcggag agatagccaa agaaaaagta cagttatttc ttgaaagaat aatgaactat
    14461 aaaacaaaag cggagaacaa ttgttaactg atataatccc cttaaagcag actcttgaaa
    14521 aacatggttt tcagggaatg ctttaagggg agatttttat gtaaaaaaag aaaaaaagta
    14581 cggtactgaa acgggataaa gacaactgtt aaatcatatt ttcggatttg acaataccat
    14641 gttacaaaaa atccgcagac ggtaaaatct gcggataaaa tgtttaaaat tataattaaa
    14701 atgcctctat aagcggattg actgcacccg gctgtatttt ttcgggatgg tcaatatatt
    14761 cgattgcctt gccgcaaccg ttgataacgc agtcagcggc ggctttgtga actcttactt
    14821 taagctttgt ggattcgctg agaagccttg caaatcccgt cagcttggca agaccgcctg
    14881 tcaaaattat accgtcagtg tagatgtcac cgacaagctc gggcggagtc tgttcaagca
    14941 catctttaat tgctctgata atgttaagcg caatatcctc gattacaggc ttgatttcgg
    15001 catagccaac atcaataaat ttgggcagac cgcttacggc atctctgcct ttaactctga
    15061 aaagcttagg ctctttcgga atttttacac agccaacagc ctttttgcag gcctctgcca
    15121 tgtttgtgcc gataatcagg ttatgctctt ttctgatgta tttaacgatt tcttcgtcca
    15181 ttgcacagcc tgccgacttt atgcactttg aaaccgaaat tccgccgaga gaaagcacgg
    15241 caatatctgc cgtaccgctg ccgacatctg caatcagaat gccgtgagga cttgttatgt
    15301 cgagcgaaca gcccattgcc gccgcttttg gtgcttcaat aagatacacc ttgcgaacac
    15361 cgattgagct tactgcgttt acaacagctc ttttttcaac ctcggtaacc tcactcggga
    15421 ttgatgcaac aaccctcggc atagtcacct ttgacggact gacctctttt ataagaattg
    15481 aaaccatatc ctcaacaagt tcggagcgtg aaataacacc gtcatcaatc gggtgcattg
    15541 ctcgtatgcc tgcaggcgtt ttgccaatca tcttataagc ctcgttgccg acgccgacaa
    15601 tgtcgtaggt gtcaacatca aacgcaacaa cgcttgcctc atcaagcact attcctttat
    15661 tttcaataaa aactcttgtt ctgtcagaac cgagatcaac agctatatcc attttaatca
    15721 ctccaatgct atatcagcag agatgtgcaa agcgtacagc aacaaatctc gccgcatttg
    15781 cacttgttaa gtattcttgc acattcacca agcgtactgc ctgttgtaat aatgtcgtca
    15841 ataatcagta ttcgcttgcc tttgattaaa ttcttgtcaa tcggcgagta aacgcctata
    15901 acattttttg ccctttcaac cgcttttatg ctgtgctggg ttttattttt ctttgttttg
    15961 gcaagcaggt cggcatactc aatgccgaga agccttgcac attcctttgc aagcaactca
    16021 gcctgattgt atccacgctc tctgagagta tccttgtgca tcggcacaca ggtgacaagg
    16081 tcaaattccc tgtcacggta aacatctttt attgaacgaa ccatttcgtg tgaaagcaat
    16141 tcggcatatg caccgcagtt gtgaaattta aagttaagaa ctgccctgcg gaaaattccg
    16201 tcatagggga aaggagaggt gcagatgtac cctcccgttg catatcgtac aagcggcatt
    16261 tcggggaatt cttttctgca tttgtcacaa cagcttttgt tcggttcaat aacccttccg
    16321 cagtacggac aacgaaccgt aaagaaaaat ccttttatcg tgccgaaaac tttcttaata
    16381 aagtgtgtca tctctcagta aaaattcttt aagaccgctg tacctttttg tacggcggtt
    16441 attgtttacc atatattcaa cggttgacgg attgccgaca agcacaagcg ttttcttcgc
    16501 ccttgtaaca gcggtgtaga gcagatttct gtagtaaagc tgaggaggtc ccgaaaacat
    16561 cggtataata actgcgtcaa attcattgcc ctgactttta tgcaccgtga ttgcgtaggc
    16621 aaaatcaaga tcgcccgcaa attcaaaggt gtatcttgca atcttgtcaa aaaatttaac
    16681 gacaagcgat tttgtctttc tgtcaatctt tgtgatgatt cctatgtcac cgttgaaaat
    16741 tccttcgccc gtttcgccgt tttcacgaaa ccagcgaata tcatagttgt tttttatctg
    16801 cataaccttg tcgcctgtac gaaaggtttt tgaaccgatt gtgatttccg ccttatcgtc
    16861 atccttcgga tttagcgccg cctgcaattt gtggttaagc tcggcagttc cgagttcacc
    16921 ttttttggac ggagcaagca cctgaatgtt ttcaaacgga gaatatccgt atgcctttgg
    16981 cagacgattt gcacaaaggt cagctattac cgctgaaact tctgatttat tgttcctttg
    17041 aagaaagaaa aagtccttgt cggcggaatt aagaatgggt atttcaccgt tcacaatctt
    17101 atgtgcgttt gtgacaataa ggctctgctg tgcctgacgg aaaatttcat tcaaccgtac
    17161 aacgggaata agtccgcttt cgataagatc gccgagcaca ttacccggac ctacggaggg
    17221 gagctggtcg gagtcgccta caagaataag tctgcatcct gtggaaagcg ctctcataac
    17281 gctttccata atgaatgcgt ccaccattga aacctcgtca acaatcaatg cgtcgcattt
    17341 aagctgattg cgttcgttct tgttgaaaac ggggttgtca tgtttatccc agctgacctc
    17401 aagcagtctg tgcagagttt ttgcctcatc gcccgtgagc tcgctcattc gttgagccgc
    17461 tctgcctgtg ggggcagaaa gcaaaacagt tttgccgttt gctttcatta tttttattat
    17521 agcattgagg gttgtcgttt ttcctgtacc gggaccgccc gtcagaacga gcataccctc
    17581 ggtaagtgcc atagtgattg ctttcttttg caaatcggca tattcgattc cgtcctcttt
    17641 ttcacatttt tcaatcgcct tgtcaatgtt tttaattttc tctgacggaa atttaagcaa
    17701 catttttatt cttgacgcta tgtacatttc gcagaggtga acattggggg ggaacacaaa
    17761 ttttttgccg tcaatcttat cttcgataag cgaacggtca aacagcattt cttccatgca
    17821 ctccgaaacc ttttcaccgt caattccgag aaaatcggtt gaaaccttca gcagcttttc
    17881 ctttggcaga caggtgtgac cgtttcgctc gttgtgatga agaatgtata cgattcctgc
    17941 acgcatacgg acatttgaat cgggttctaa tttttctttt ttagcaataa aatccgcact
    18001 ctcaaacgat accccaaaat caccctgaca gagaatgtac ggatttttct tgattaaatc
    18061 gacacagctc ggaccgtaag cgttgaaaat tttgaccgat gcggtgtttg aaattccgta
    18121 ctcgccgaga aaaagcatga gtgttcgcac gcctgtgttc tgctttaact gctttgaaaa
    18181 atcctcggct ttttgcgaag aaattccttt cagcattgca actctttcgg gctgattttc
    18241 aagcacatca agcgttgcct cgccaaattc ctttacaagc ctttgtgcgg tctgagtgcc
    18301 gattcccttg atagctccgg atgacagata acgcagaata tcagccgcct cggtaggtct
    18361 gctgatttcg caaatctgca cggcaaactg tctgccgtag ctcggatgat ttgtatattt
    18421 gccggtaagc tttacaacat cacccgacga cacctgtggc ataattccga cagcggtcaa
    18481 ataatcgtca ccgtcagcaa gctcaattac cgtgtagccg ttttcatcgt ttcggaaaac
    18541 cacgctttca attgctccct caagctgaag aagattgtcg ttgttcatta aaacttactc
    18601 ctcaaatctt tttgtgagtt cgccgagagt aatttcgtag cccgctcttt cgtagtcaag
    18661 gtaatccgta cacggcttga agccatccgt attttttgaa atctgcaaag caatgtattc
    18721 aagaaaatgc ggatttttaa tcataatagg acctgtaaca tgagtagcaa aaaggttctt
    18781 gtccttcaag ccctctgctt ttgcattttc gtagtcaccg agtccgtgtt taacagtgaa
    18841 aagatgattt tcaatacctt taatttcact gcacttgttt acaaagccga caatcggttt
    18901 tgtgagaatg tcagatgtat aaacaatatc gcctgtaatt ctgcgtttgt tctgctcggt
    18961 aactgtaaaa tcataaatcc cgagtccgtc aaaaaccttg ccgtcagagt ctgtaattgt
    19021 ttttccgagc atttcaaacg agttgcccgt cataagaatt accttgccgc tgttgatgta
    19081 ttcaacaaga aaatccttgt actttttaaa atcctgcaaa acaagcttct gatttttttc
    19141 tgtacccgaa ccgatgtaga taaaatcgta atcgcccagg tcggcattgt caaacagggt
    19201 aagcctgtcg gttgtaaatt ttacaccgct cttttcaagc attctttcaa gcgcagaaac
    19261 attggcatat tctccgtaca ggttcattat atcgtgatac aaatgaagaa ttttcataat
    19321 cagtccacct ttaccttttc aagaaattta cccttgtctg agaaacaggt aattacataa
    19381 atcttttctt tgcggtcatt gtcaagataa tcaacggctt cttcaatgtc cttgataacc
    19441 ttgattttgt ttctgtcaac ttttgagaaa gaaaatcttg ttgcaaggtc gttgcaatat
    19501 gttcctgcaa gcacaatgtt ctttacattg tcgcttttga gaatgtcaaa gttgatgtcc
    19561 caaagccatg aaacatcgct tgtaaaatac tttctgctga ctgcgtcaac aataatcaaa
    19621 acatcgcact tatccttgtc ggatgcggca agcttgagtg actgattgta cgaaatgctg
    19681 ttttcatgtt ttgatgtaac aagcgtaccc tttctgtttc cgagcgtgaa tgttacaaca
    19741 cggaagtttt taagaacata attgctcagg ctctttgcaa ttgtgttgcc gtcaacacct
    19801 gtgattgacg caacggtaaa tgccgcaagc aaattgtaaa cattataaag cgatttgagc
    19861 gtaagttcaa tcttgtactt attgttgatt gtaatttcgc ctttttcaag atcggcagat
    19921 gtaactgtgt agcttgtatc ctgtctttta agaccgcagt tagggcattc atagttgcct
    19981 atgtggtcgt agtgatagct tgaatatgtc ataggagatt tgcagtgagg gcaatatgca
    20041 ccgtcattat aaacaccgac aaactccttt gtatcggtcg gaagattgcc tgcaccgaac
    20101 catacaacat tatccctgcc gtagccaaag cacgaaacaa gcggatcgtc ggcgttaaga
    20161 ataagctgag tttcgggatg aatactatcc tttacggcat tgtaaaccca ttccggatga
    20221 ccgtttcgtg tgagctggtc acggtaaagg tttgtgataa cataatgagt cggtgtgatg
    20281 tatttgaagg tatattttgc aaagcgttcg tcactttcaa tgagaagaat atccgattta
    20341 accttgcccg aaagagtaca actgccgaga ataagagttg taacgccctc aatctggtta
    20401 gaaccctctt tgttccaggc aacttttttg ccgctttcac aaagaatatg tgcaatcatc
    20461 tcaacggtag atgtcttgcc gttactgcct gttacggcaa caatatattt cggcagctca
    20521 attcgtccga gaatatcggg gcagagttta agcgcaatct gtcccggcag actgctgccc
    20581 ttgccgagca atcctccgac aaatttcaga accttgcaca caataatagt aaatagtttt
    20641 ttcattttgc cacttcctta attaaagaca acctgtgttg cctctacata ccttgtatat
    20701 attcttcatc attataccac aattttattt ctttgaatat atattcttaa aattttttgc
    20761 aaattgaaac agcaggttga aaaaatttta aaacccaaaa ataaaaagcc cgaaattcgg
    20821 tcagcttgaa tttcgggctt ttctgcagac tgcaaccatc cgttcaacga gtggttttga
    20881 ttttatctgt acttttcaac aaaatcggcg ataaactttt tgaaaacact tgcctgtgaa
    20941 attccgatat ttctgcatat ccctctgaat tcctcaacaa gttcctttgg ggcggaaaat
    21001 gaaatttgac tgtaaacttt ttgattgtat ctgcgtttta cttcggttga tgtgacggtt
    21061 tttctttttt cttccataat aattcctcct caaatcaaaa cggaatctca taatttaatc
    21121 gttttttctg caaattccat tgatattttc aatgtaacta tattatacaa aagcctgctg
    21181 tcccattatc ctccctatga attttaaaat tgaattttgt tgtcagttga aaagtaaatt
    21241 aatttttgga tttacttgta ttgaaggtga gattataata taataagcgt tgttatgtaa
    21301 attttgttat gtgacttatt aacggagaaa attatgactg aaaaagaaaa aatgcttaat
    21361 tcgcagatgt ataatccaat gggcgacaaa cagcttcgca atgaacgctg ttcggttaaa
    21421 aatctttgca gagagtacaa caatcttccg tttgaagatg aagagactca aaaaattctt
    21481 ataaagaaaa ttgtcggcag tacaaaggaa aatttctaca ttaccgctcc gttctggtgc
    21541 gactacggct acaatatcga actcggtgag aatttctatt caaaccacaa tatggtgatt
    21601 ctcgactgtg caaaggtgaa attcggcgac aatgtttttg ttgcaccaaa ctgcggattt
    21661 tacacggcag gtcatcctgt tgattatccg agaagaaacg caggctttga atatgcctat
    21721 ccgattaccg tcggcgacaa tgtgtggatt ggcggaggag ttcaggtcat gccgggcgtc
    21781 acaatcggaa gtaatgttgt aatcggcggc ggaagcgttg ttgtaaagga tattccgtcc
    21841 gactgcgtag ctgtggggaa tccgtgcaag cctatcagaa aaatcacgga tgaggatatg
    21901 aaaacctgtt ttgacaaaag tatgaataaa taacttcaag tgaggatttt atggagctta
    21961 aaagtaacga agaaaaaatt acccgtgcat tgctcgtcag tgttgacacg ggcgaatatg
    22021 acgcacaggc gtcacttgac gaactttttg aacttgtaaa aagtgcggga gcagatcccg
    22081 ttttgtcggt aacgcagaat ctgcaaaaaa ttgaaaaggg tacttttgtc ggcacaggca
    22141 agcttgccga gattgccgaa atctgcgaat cgcaggagat agatttgctt gtgttcgaca
    22201 gcgagctttc tccgacacag attaaaaata tcgaggctga aacagatgtc agagtaattg
    22261 accgaacaac gcttattctc gatatttttg cccagagagc aaggtcaaaa gagggtaagc
    22321 ttcaggttga gcttgcacag cttaaatata tgctcccaag acttacgggc aagggtatcg
    22381 caatgtcaag actcggcggc ggtatcggta caagaggtcc gggtgaaaca aagcttgaaa
    22441 ccgacagacg acatatccgc agaagaattg aaagcctgaa agaagagctt tccgaccttg
    22501 aaaagcatcg tcagatgttg cgttcacgca gaaaaaaaga cggtgtaata acctgtgcga
    22561 ttgtcggcta cacaaacgca ggcaagtcaa ctcttatgaa ctgccttacg gacgcaggtg
    22621 tgcttgcaca ggacaagctt tttgcaacgc ttgatcccac ctcaagagcg ttgaagcttc
    22681 cgagcggtgt aaccgttatg atgattgata cggtaggtct tgttcgcaga cttccgcacc
    22741 accttgttga agcattccgc tcaacccttg aagaggcggc acagtcggat attattctaa
    22801 atgtatgcga cgcatcaagt gacgaggcaa gaacacatat gcaggttaca accgacttgc
    22861 ttgaatccct cggctgcggt gacacaccga ttataaccgt tctcaacaag tgcgatttgc
    22921 ttgacgaaac tatgcttgca caggacttta aagcctgtgt tcgcataagc gcaaagaacg
    22981 gcacgggtat agacgaactt cttaatgcaa ttgagaacaa tcttcccgtc agaatgaaaa
    23041 gagtaaaaat tcttctgccg tttgcacagg caggtcttgc aaacgaaatc cgcaacaaag
    23101 gcactctcat ttatgaagaa tatgttgccg aaggactttc ggttgaggct gttgttgacg
    23161 aagcacttta tgctaaactt gcaaaatatg aatgtgaata acttaaaatt tatttaattt
    23221 gacattctgt tttttggttg ttataatgat attatgaaag gaaaatgata tgtcattctt
    23281 tgatatgatt caatcatttg acacctcggt tcttgaattt attcagaaca catttaaatg
    23341 ctcttttctt gatccgatta tggctttttt cagctatatg ggtgaaatgg gacttttctg
    23401 gattgcagtc ggcattgtgt ttataatttt taaaaaaact cgctcaatgg gagttatgat
    23461 gcttgttgca atggcaatcg gctttcttgt cggtgaaatc ggaataaaaa atcttataaa
    23521 ccgaccacgg ccgtttatgg tgaatatcga ttattcagca agaccgtctg agttgaatcc
    23581 cgactttggg cttaacatag ccattccgtc aggcagcagt tttccttcgg gacacagctg
    23641 ttcgtcactt gcggcggcaa ccgttatgct catcaaggac aaacgattcg gaattcctgc
    23701 acttgtactt gcgcttttga ttgtattctc aagactttat aactatgttc attatccgtc
    23761 agatgttctc tgcggaatgt tactcggcgt gatttgtgcc gttgtcgttg tgcttgtgtt
    23821 taaaaagaca ggtcttgaca acaggctgtc accgcaaaag gtcaaagaag gctgatttta
    23881 tgaaaaatta cagatttgtc ggcaaaacct caaaagagac ttttctcatc aagggaatta
    23941 atgttttcaa cgagcgttgg attcatgagg gcagttgtgc caccgtgaca aatcctcaga
    24001 acggcaaaag ctatgtattt tcaaagtaca taagcggagg agttgagttt gttgcgggaa
    24061 aggacgcaag cggttactgg ctgttctttg ttgaggtata aaactgtaaa attctttgaa
    24121 agaataatat atattgggag taaatgttaa aaatggaaaa cgaaaaatta ttggcaaaaa
    24181 atctgtttga ttacagcagg actattgcaa agcccttgct tgaaaccgtt gattatccgt
    24241 gggaggctct tccgaaaatc agcgaattta tcattgaact cggaaaaact cttgatcccg
    24301 aaatctacga gcagagggga gaaaatgtgt gggttgcaaa aagtgcaaag gtttttgact
    24361 ccgcatatct cggcggaccg ctcattatct gcgaagatgc cgaaatcaga cagtgtgctt
    24421 ttatcagagg caatgcaatt gtcggcagag gtgcagttgt aggcaactca acagagctta
    24481 aaaattcaat tctttttgac ggtgttcaga ctccgcacta taactacatc ggtgacagta
    24541 ttctcggcta caagtcacac acaggcgcag gtgtaatcac atcaaacctc aagagtgaca
    24601 agagccttgt aactgttctt tgcgacggcg ataaggttga cacaaatgtt aaaaagttcg
    24661 gcgcaatgct cggtgaccat gtagaggtag gttgcaacag cgttcttaac cccggttcgg
    24721 taatcggcag aaacaccaac atctatccgc tttcatttgt aagaggttat gttcccgaaa
    24781 acagcatcta caagcgtctt ggcgaggttg ccgaaaagat tgaataatat ttatgcattt
    24841 gtcggcaagc ataattattg tgtagttttt atgcgataaa ggaaaaataa cagattttaa
    24901 atttttgcgt ttttttgttg acaattttga tgttatttgt tacaataacc aaggttttga
    24961 tgacttttat cagaacaatt ttctgccgtg tgcagaagtt ggattgtata agcccgtaat
    25021 acggacgggt gtttctacaa actgccttaa acagtttact ataatccccg ttaaggggcg
    25081 tagtattatg ttttggcggt ttttttattg cccttaaatg ttacccacaa caaataaagg
    25141 agaaaatgat aatgaaaaag tacgatgtta ttattgtagg agcaggtccg ggcggaattt
    25201 tttctgcata tgagcttgta acaaacaact ctgaaataaa ggttgccgta tttgaaaaag
    25261 gtcatcttct tgaaaagaga aaatgtccga ttgacggtga caaagttaaa aattgcattt
    25321 catgcaaaac ctgttccatt atgagcggtt tcggcggtgc gggtgcgttt tcggacggca
    25381 agtacaacat aacaaacgat ttcggaggca cgctctacga acatatcggc aagaaaaaag
    25441 ctattgagct tatggaatat gttgacaaaa tcaatatgaa gttcggcgga gagggtacaa
    25501 agctttattc cacagcaggc acaagcttta agaagctttg cttacagaat aagcttaatc
    25561 ttcttgacgc atcagtccgt catctcggta cggacatcaa ctacattgtg cttaaaaatc
    25621 tttatgatta ccttgaaaac aaggttgatt tctactttga tacacctgtt gaaaatgttg
    25681 aactcaagaa cggcaatgat aaggaaggtt atcttgttga cacagccgat gaaaattatg
    25741 agtgcaaaaa gtgcattgtt tcagtcggca gaagcggcag caagtggatg gaacatgttt
    25801 gtcataacct taacattccc acaaagtcga accgtgttga catcggtgtt cgtgtggagc
    25861 tccctgccgc aatcttttca cacctcacag acgaactcta cgaaagcaag attgtttacc
    25921 gcacagaaca gttcgaggac aatgtgcgta cattctgcat gaatccgaag ggttgcgttg
    25981 taaacgaaaa cacaaacggc attatcacag taaacggtca cagctatgag gacaaggcaa
    26041 agcagacaga gaacacaaac tttgcactcc tcgtcagcaa gcatttttca gagcctttca
    26101 aggacagcaa cggttatggt gaaagcattg cccgtcttag caatatgctg ggcggcggtg
    26161 taattgttca gcgtttcggt gatttgattc gaggtcgccg aagcaatccg agcagaatca
    26221 aagaaggact tgttgttccg actcttgacg caacaccggg cgatttgagt cttgtgcttc
    26281 caaagagaat ccttgacggt atcatcgaaa tgatttatgc tcttgacaaa attgcccccg
    26341 gcacagccaa cgatgacaca cttctctacg gtgttgaggt caagttctat aatatggaag
    26401 ttgatgttga tgagcacctc gagtcgcccc acaagggact ttatatcatc ggtgacggca
    26461 gcggcgttac acattcactt tctcatgcca gcgccggcgg tgtattcgtt gcaagggaaa
    26521 tcttagcaaa ttaaaaattg acggcgagcc gccgttccca attcgtgaag atagattgca
    26581 taataaacag catattatgc ccgagtgcaa aattgcaagt gtttatgcag tctatgctgt
    26641 aggggcgttc attggacgcc cgcattgatg taacattttg tttattcaca taacttatga
    26701 tttaatcaaa aatgtgtatt catttgcagc ggtgaacaag gtttgccgct gcaaaacata
    26761 cgatatcttt ctgtgactgc aaacaataaa aataaccaaa acctattgat tttattaatc
    26821 ggttatggta taataagctc gttgtaaagc agactgaaca atcagcttgt cttgcaatgg
    26881 tgttcttttg tacaatatat ttctttttac gaacaaagaa atgaataatt tagttcagaa
    26941 ttgcaagaag tcaaaaagcg tgcaattaag tgagcgacag gccctgtacg attggggtct
    27001 gtgaatcatg tcaggcgggg aaccgagcag cattaagcag tattgccgat gcgatgcagt
    27061 aagtctgtcg ttcactcatt tgtacgattg ccgttatttc ggctgacggc ttgcattttt
    27121 tgttgattgc ggggtgaatt tttgtatcag gtactctata gaaagtggcg accgaaaacc
    27181 tttgccgatg tttcgggaca ggaacacata accacaactc ttttaaacga aatcagcacc
    27241 aaccgtctta accatgcata tctttttacg ggttcaagag gtacgggcaa aactacctgt
    27301 gcaaaaattc ttgcgaaggc agtcaattgc cttcatcccg taaacggaaa tccctgcggt
    27361 gaatgtgaaa tctgcaaggg aattgacgac ggcagtatcc tcgacattgt cgaaatggat
    27421 gccgcatcca acagaaaaat tgatgatatc cgtcagatta ttgacgaggt tcagttcaag
    27481 cccgcaaagt gcaaatacag agtttacatc attgacgagg ttcatatgct cacaaccgag
    27541 gcgttcaatg ccttgctcaa aacgcttgaa gaacctcccg aacatgtcat attcattctg
    27601 gcaactaccg aagttcacaa gcttccgcag acaattctgt cacgctgtca gcgtttcgat
    27661 tttcacagaa tcccaccccg tgcaatagca gacagactgc tctatgttgc aaatcaggag
    27721 ggcgtgacac tgagcgacag tgccgcaatg cttgccgcat ctgttgctga cggtgcgttg
    27781 cgagatgctc tttcgcttct tgacagatgc attgcaatca gctcggaaat tgatgaagat
    27841 gttgttcgca gtgccgcagg acttgcgaga aagacatatc tttttgaact ctctaactgt
    27901 gtaatcaata agaacacagc aaaggcactt gaaattgttg acaggcttta cggtgagtcc
    27961 aaggatatgg caaggctttg cgatgaattg ctttcgcatt tcagaacgct tatgctcata
    28021 aagtcggtaa aaaatccgag ggatatcgtg attatggctg acgatgagtt tgaacaggct
    28081 caaacgcagt cggattacct ctcacttgcc gacattgttt attatatgga tgtgctttca
    28141 aaggcatatc agaatatggg caggggaaca ggcgacagaa cagagcttga aatggcggtt
    28201 gtaaagctgt catcggcaga gcttgacggt acaattgagg ctttgaccgc aagggtaacc
    28261 gctcttgaaa aggctgttaa aaagggaata aaggtgaatt atgttcccga acaggtgcaa
    28321 actgtaccgc agtctgttca gaatgaaact ccaaaaccgg aggttcaaaa ttcagaacct
    28381 gaaaagtcgc aggaaacaaa acctgtttcg gaaaaaatca aggacgagcc ggtaattccg
    28441 acgcctcctg ttgagatgcc gacaccgaaa cagcccgttc agccaataca ggaacaaccc
    28501 gtttctgcac agcaaaagcc taagaaaaca gctcaaagac agagcgttga ccttgatgca
    28561 atctataata acgcacagcc ttttcccgat tgggcagagg tagtcaacaa tatgaaacct
    28621 gtttcaaggg caattgcggc ggcatttgca aattctgccg cttacacaag cggaaaatat
    28681 ttgctgattg acacagataa tgaaatggct tttgaacttc tcaaaaataa cgaaagacga
    28741 caggaaatcc gtcagcttat tttagaaaca acaggtcagc actataatct cggtccgtat
    28801 aaaagaccga gtgcaaaaca agaagaaaaa accgatcccg ttgacaagct tattgaaagc
    28861 ttgcagggaa gcgatgtaat aataacacaa gaataaccga aaggaagata aaattatgaa
    28921 agcaagatta ccaaagggtt acggcagcgg cggagcaaac aacattcagc agcttgcccg
    28981 tcaggctcag aaacttcagg atgatatgga ggccgcatca aaggaactcg aaacaaaaga
    29041 gtatgaggca acagccggcg gcggtgcagt taaggttaca gtaacaggca aaatggaact
    29101 cacaaaggtt gagatttcac cggaagttgt tgatcccgag gatgtagaaa tgctctcaga
    29161 cctcgttatg gcagccgcaa atgaggcaat cagagcagcc gctaaggaaa aagaggacaa
    29221 aatggaaaca atctcaggcg gacttaacat tccgggaatg ttctgattat gtcgggatat
    29281 aatgttgctc cgcttgaaaa tttggtggag cagtttgaaa gaatgccggg aatcggtaac
    29341 aaaaccgctc aaagacttgc atattatgtt ctcaatctgt caaaaacaga agcggaacag
    29401 cttgcgggtg cggtgatgga tgcccacaca aagattcact attgcagtaa atgctgtaat
    29461 cttacagaca aagacctttg tcccgtgtgt gcaaatcccg caagggatca ttccgttatc
    29521 tgcgttgttg aaactccgag agatgctacc gctattgaaa atacccacga atttaagggt
    29581 gtttatcatg ttctccacgg ttcaatctca ccgcttaacg atatcggtcc cgacaacctt
    29641 acaataaaag aactgcttgc aaggcttaac gaggaatcgg ttgaggaggt cattatggca
    29701 accaacccga ccgttgaggg cgatgcaacg gctctgtata tttcaaagct cttaaagccg
    29761 cttggtgtaa aggtgacaag acttgcctac ggtatccccg ttggcggtga ccttgaatat
    29821 gccgatgaat acacccttgc aaaggctctt gagggcagaa atgaaattta atttcttgat
    29881 ttaaactat
//
LOCUS       DAOMVE010000005        27251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_264_length_27251_cov_4.238013, whole genome shotgun sequence.
ACCESSION   DAOMVE010000005 DAOMVE010000000
VERSION     DAOMVE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 27251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27251
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_264_length_27251_cov_4.238013"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..1910)
                     /locus_tag="PLS64_00615"
     CDS             complement(264..1910)
                     /locus_tag="PLS64_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL42365.1"
                     /translation="MQLYKNFCKETTDENGKLTSFELVFPENYNFGYDVVDKMAELVP
                     NDTAVVWTNPEKEEKIFTYSDISKLSNKAANVFRNHGIKKGDKVLVILKRNYEYWYVA
                     PALHKLGAVIIPATNLLTQSDISYRVNTAGIKAAVCTPDETTAEDILAVASQPNTLET
                     VFVPRKQIFGAVDFTEEVENAPDTLERVETKAEEPMVIYFTSGTTGYPKAVEHNHIYT
                     LGHIITAKYWQCVKDGGLHLTVAETGWAKASWGKIYGQWLCGSAVMVYDFDKFSPGKL
                     LRIMEKYKVTTFCAPPTVYRYFIKRGMNKYDLSALSHLTTAGEALNNEVFEEVFKQTG
                     IELCEGFGQTESVLMLANLKGDKAIAGSMGKPTPLYDVRIVNDECNEVKQGEVGEVVV
                     FPPKDRKQHGIFITYYNNENLYEKVWRHGVYHTGDTAYKDENGYFWYVGRADDLIKTR
                     GFRVGPFEVENVLMQYPNVLECAVIGVPDKDRGQAIKAIVKMTDGAPHDKELENKIKT
                     FCNKRMASYKWIQHLEIVDEFPKTISGKIKRTDLRENHKA"
     gene            2369..3436
                     /locus_tag="PLS64_00620"
     CDS             2369..3436
                     /locus_tag="PLS64_00620"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRL42366.1"
                     /translation="MRIVVLAGGLSTEREVSISSGTLVAEALRSKGHEVVLLDVFMGY
                     EENICDIDALFKQNYSFTDGNSIGDDVENTITNIKEAKENRLDKTSRYIGRNVIEICA
                     EADITFLALHGGEGENGQLQATLDLFGIKYTGSGYLGSALAMNKGLTKSVFMQKNINT
                     PSGELYKNEKDALAWNMFPCIVKPCSGGSSVGVSKVENADQYKKAVKEALKYEDEIVV
                     EQFVTGREFSVGLIGGKALPPIEIAPKSGMYDYAAKYQAGATVETCPADIDEETDKKL
                     RDAATEAYEALHLESYARIDFLLDKGGFTYCLEANTLPGMTPTSLLPQEAAVEGISYP
                     DLCEKIIEISLAKYPKEKSDF"
     gene            3449..4801
                     /gene="murF"
                     /locus_tag="PLS64_00625"
     CDS             3449..4801
                     /gene="murF"
                     /locus_tag="PLS64_00625"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRL42367.1"
                     /translation="MKDFTLAEIAKACNGEYVGDESLKNTKITSVERDSRQVKNGSLF
                     LAIKGERVDGHDFIEKCFAQGAVCALCEKAPENATKPCIVVPSTLDAVKKIGRAYREK
                     FDIPVIGISGSVGKTSTKEMLYAVLSQKFKTHKTQGNLNNELGVPLTLLSMPEDTEAA
                     VIEMGISDFGEMTRLSEMVQPTICVLTIIGECHLENLGDRDGVLKAKTEMFKNARENA
                     DFILNGDDDKLSTVKTVNGKKPVFFGLDAKNDFYAKNIKNSGDGKVLATLCFDNNDID
                     VEIPTIGTYMVTNALAAAAAGKLLGLTDEEIANGVASYKTVGSRANVINTGKIKIIDD
                     CYNANPTSVRASLDTLSNLDGRKVAILGDMKELGSEELKLHFDTGVYAKSKDIGTVIT
                     VGELAKELAKGADGKAFDTIEDAKPEILKTIKSGDVVLVKASHSMQFENIVEFLKQNF
                     "
     misc_binding    4860..5109
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5170..6630
                     /gene="trpE"
                     /locus_tag="PLS64_00630"
     CDS             5170..6630
                     /gene="trpE"
                     /locus_tag="PLS64_00630"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HRL42368.1"
                     /translation="MIKPSYDEVLNLKDNYSVIPIYKEIYADAFTPINLLRKIAGKSD
                     KFFLLESIEGGEKWARYSFIGFNPKARLSYKNGTLTVTGEGARVVKTAKPYDALREYL
                     ADYKTPHFKDIPPFTGGLVGYFGYAMISVAEPVLKLKRGDTNDFDMMLFDKIIAYDHL
                     KEKLIIIVNMKTNDTKAQYELAKKDIAEIISTITSPEPLPKLESDGNVEFTSTYSKEE
                     FCKMVEKTKEYIFDGDIFQCVVSRRFEADYKNSLINAYRVLRSTNPSPYMVYMSIDGD
                     EIISTSPETLVKLQNGVLNTFPIAGSRPRGKTDTEDNALADELIHDEKELAEHNMLVD
                     LGRNDIGKISEFNSVKVTKYQEVLRYSKIMHICSEVEGKLKDGLDAFDAIESLLPAGT
                     LSGAPKIRACEIIEELERTPRGVYGGALGYVDFNGNLDTCIAIRMAVKKNDKVYVQAG
                     AGIVADSVPESEYEETYNKALAVMNAVKNAGEVNAL"
     gene            6627..7199
                     /locus_tag="PLS64_00635"
     CDS             6627..7199
                     /locus_tag="PLS64_00635"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRL42369.1"
                     /translation="MILFIDNYDSFTYNLVQFCGSVNPDIKVVRNDEITVDEIKKLAP
                     SHIILSPGPGYPKDAGICEEVIKELAGQFPILGICLGHQAICEVYGATIAHAIKLMHG
                     KKSDIIVDTDKKIFNGLPKVVEGARYHSLIAKRDTVPDTLEVIAEDRDGEVMGVKHKD
                     FELYGLQFHPESILTSHGMEMIKNFITLCK"
     gene            7221..8231
                     /gene="trpD"
                     /locus_tag="PLS64_00640"
     CDS             7221..8231
                     /gene="trpD"
                     /locus_tag="PLS64_00640"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRL42370.1"
                     /translation="MIKEAIKKLVAGNDLTFDEAAQVMDEMFSGTATQSQMAAYLTAL
                     RIKGETIDEITASAQVMREKALHIKPNRDVLDIVGTGGDGTGTFNISTTAAFIIAAAG
                     IPVAKHGNRSMSSKSGSADCLEQLGININITPEKSEEVLNKAGICFMFAQGYHSSMKY
                     VGPVRKEIGIRNIFNVLGPLTNPAGADLQVTGVYSEALVEPIAQVFSNLGVKKGYVFY
                     GMDGMDEVTLTTTTKVCEIDNGKFNTFILNPEDYGLKLCAPEDLAGGDGKENAEITKE
                     ILSGEIKDAKRDIVVLNAALGLCTGGKADSIQDGIKLANEIIDSGKAYAKIEEFAKAS
                     NE"
     gene            8242..9024
                     /gene="trpC"
                     /locus_tag="PLS64_00645"
     CDS             8242..9024
                     /gene="trpC"
                     /locus_tag="PLS64_00645"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HRL42371.1"
                     /translation="MILETIADANRVRYEEIEKQVPLEVIKQKALSMETDNEFQFEKA
                     LAGKDISFICEVKKASPSKGIIAEDFPYVQIAKDYENAGTSAISVLTEPKWFKGENAF
                     LEEISKNVKIPLLRKDFTVCEYQIYEAKTIGASAVLLICSLLDTDTIRRWIKLCDSLG
                     LSALVEAHTADEVYSAIAAGARVIGVNNRNLRDFTVDINNCTKLRKLVPDNIIFVAES
                     GIKTRDDIKVLENAGVNAVLIGETLMRNPDKKAALDELSGRK"
     gene            9090..10274
                     /gene="trpB"
                     /locus_tag="PLS64_00650"
     CDS             9090..10274
                     /gene="trpB"
                     /locus_tag="PLS64_00650"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRL42372.1"
                     /translation="MTKGKFGIHGGQFVPETLMNAINEFEEAYNRYKDDPEFVAELDT
                     LMREYAGRPSLLYYAEKMTKDLGGAKIYLKREDLNHTGSHKLNNCLGQCLLAKRMGKT
                     RVIAETGAGQHGVATATVAALLGLECEIFMGKEDTIRQALNVYRMKLLGAKVNAVETG
                     TMTLKDAVNEAMREWTNRVSDTHYVLGSVMGPHPFPTVVRDFQSVIGKEIKSQMLEKE
                     GRLPDVVMACVGGGSNAMGAFYEFIKDENVKLIGCEAAGLGTDTPKTAATIATGTLGI
                     FHGMKSYFCQNEYGQIAPVYSISAGLDYPGIGPEHANLADTGRASYVPVTDEEAVNAF
                     EYLSRTEGIIPAIESAHAVAHCIKIAPTMDKDKIIVVNLSGRGDKDVAAIARYRGEDL
                     HD"
     gene            10267..11037
                     /gene="trpA"
                     /locus_tag="PLS64_00655"
     CDS             10267..11037
                     /gene="trpA"
                     /locus_tag="PLS64_00655"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRL42373.1"
                     /translation="MTDIKSAFQNGKAFIPFVTAGDPDLETTEKLLIEMSKNGADIIE
                     IGIPFSDPIAEGVVIQEADLRSLSAGTTTDKIFDMVKRIRPQIDAVLAVMTYMNPIFV
                     YGTERFMAKCQECGISAVIVPDTPYEEKHELTDYSGKYGIDVISLIAPTSHERIKMIA
                     KEAEGFVYCVSSMGVTGVRSEITTDVGEMVRLVKSVKDIPCAIGFGISTPEQAKKMSE
                     SADGVIVGSAIVKIIAKYGKDSVKPVCDYVKSMKEAIS"
     gene            11034..11759
                     /locus_tag="PLS64_00660"
     CDS             11034..11759
                     /locus_tag="PLS64_00660"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HRL42374.1"
                     /translation="MSTLEELQSVIDKSKRIVFFGGAGVSTESGIPDFRSVDGLYNQK
                     YDYPPEQILSHTFFVHKTSEFFRFYRDKMLCLDKKPNKAHYKLAELEKAGKLSAVVTQ
                     NIDGLHQAAGSKRVYELHGSVLRNYCTKCGMFYDAEFIKNSADIPKCTCGGIIKPDVV
                     LYEEGLDDSTVTGAVNAISKADTLIIAGTSLTVYPAAGFIRYFGGDNLVLINRDPTPM
                     DKNANLVFHEKVGELLDKITVNI"
     gene            11966..12226
                     /gene="rpsT"
                     /locus_tag="PLS64_00665"
     CDS             11966..12226
                     /gene="rpsT"
                     /locus_tag="PLS64_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRL42375.1"
                     /translation="MPNIKSAKKRVKVIATKTARNKATKSALRTAVKKAYYAVDTNAD
                     NKTEAVRLAIKKIDQAAAKGILHKNTAARSKSSLAKRLNASA"
     gene            12379..12534
                     /locus_tag="PLS64_00670"
     CDS             12379..12534
                     /locus_tag="PLS64_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42376.1"
                     /translation="MKNHKCRWALIIGIISAVAALSAALTAFFIVKDKQKKDDEELME
                     YLDSSIE"
     gene            12627..12905
                     /locus_tag="PLS64_00675"
     CDS             12627..12905
                     /locus_tag="PLS64_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42377.1"
                     /translation="MIREELDRLLCDAKSNAELKEKLLKTEQSENPIDNFCSLCRSHG
                     YKISAGELFALGLDESDTKLRSVNGGGVNAIDGWDDAYEQFILTLKWT"
     gene            12908..13939
                     /gene="fliB"
                     /locus_tag="PLS64_00680"
     CDS             12908..13939
                     /gene="fliB"
                     /locus_tag="PLS64_00680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HRL42378.1"
                     /translation="MKHYFPKYCEKFRCIANLCPDSCCRDWDVVVDDTSFDFYNTVKG
                     EFGDRLRSLMTIDEDGDRIFIRQGEKCPFWNSDMLCDIYINLGENHLCHTCKEFPRIT
                     QDYTVFCEHTLSFACPEAARLMLESDFDTGFATDIPQDLQTDYSTTEMNFLLSARKRT
                     FEILSDCKMSLSERLCRLLAFNERVQNMLDDEIFDITAMPTDEYKKQSCGDASFIFDL
                     HKSLDIMSNDWLCELEATADFAKNNTLSSRFDKPLTAYLDYYVRRYYLSAIDSFNVIS
                     TIKRMVCAYIVTAYELERPNNPTQTEIVKILQGYSKEVEHSYENGELLEDEFIFNPLF
                     STDNLIGIL"
     gene            complement(13981..15552)
                     /locus_tag="PLS64_00685"
     CDS             complement(13981..15552)
                     /locus_tag="PLS64_00685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL42379.1"
                     /translation="MKQSNKRFDRHYLPLNFKLLCYFLLFGFAIVIVLWVFQTFFLES
                     FYTATKSSQVEKNAQVISKSIEENKNALGTIENVASYNSLSVYVYDSTSLFTLKYCCE
                     YDNPVSNLNFQYHDVYSYYKLAEENDGHYMFITSNYKEDSIKGKFERQFFNEYSSSDT
                     AERNTIRYASAPDKNVQNMVYTDIINLQDGSECFLIVTSSITPLTNTVEIVRDQLAIV
                     SVVFVLLAVIISLYASYRIAKPISKTNTAAKELAKKNYDVDFNARGYLEVEELNETLN
                     YAKTELAATEKLQKELIANISHDLRTPLTMITGYGEVMRDLPGENTPENIQIIIDEAT
                     RLSTLVNDLLDLSKIQSGSIQPEKSEFCLTDSIKNIFTRYAKLKEQDGYNILFESDED
                     VYIFADELKISQVIYNLVNNAVNYVGEDKTVIVTQKVNGKKVLIEVTDHGDGIPPEKL
                     EYIWDRYYKVDKEHKRGVIGTGLGLSIVKGILDSHKARYGVRSTLGKGSTFWFELDIV
                     SVKKRNKKNSDENKE"
     gene            complement(15539..16222)
                     /locus_tag="PLS64_00690"
     CDS             complement(15539..16222)
                     /locus_tag="PLS64_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020813694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL42380.1"
                     /translation="MSRILVVDDEARIRQIIRKYAEFEGFEVEEAVDGMQAIEICRKE
                     DFDLIIMDVMMPELDGFSACREIRKFKKTPIIMLSARGEEYDKIHGFELGSDDYVVKP
                     FSPKELMMRVNAVIKRSQGAQNEENVKKDVFTYEGLSVDFSARIVTIDGKRIEMTPKE
                     YELFFYMVKNKGLALTREKLISEVWGYDFFGDERTLDTHIKLLRKSLGEYSRCIVTLR
                     GVGYRFETI"
     gene            16605..17456
                     /locus_tag="PLS64_00695"
     CDS             16605..17456
                     /locus_tag="PLS64_00695"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRL42381.1"
                     /translation="MEIRKIKAQTVCDTVKKLFTDCNYFIGKDIMCALETARDNESSP
                     VGKSVLSQIIENDKIAAREEVPLCQDTGMAVLFVEYGDRVVIEDGSFDEAVNEGVRRA
                     YIDGYLRKSVVNDPVFDRINTKDNTPAIIHTKIVSGNQIKITAGGKGFGSENMSQIKM
                     LTPSKGIEGVKQFILDTVFQAGPNPCPPMVVGVGIGGTFERAAQLAKKATFRPIDSKN
                     EDERYAQLEDELLTEINKMGFGPAGLGGNTTAIGVNIETSPTHIAGMPVAVNICCHAA
                     RHASATI"
     gene            17469..18020
                     /locus_tag="PLS64_00700"
     CDS             17469..18020
                     /locus_tag="PLS64_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HRL42382.1"
                     /translation="MEKKITLPITDEDIKNLKAGDSVLLSGSIITGRDAAHKRLYELI
                     QKGEKLPVDIKGELIYYVGPAPAKPGYAVGPAGPTSSYRMDKYAPSLLDLGLKGMIGK
                     GARNKEVVDAIVRNKGVYLVAIGGAAALIAKSIKREEILCYEDLGTEAVRRYEVEDFP
                     CIVAIDSNGNNVYETEPPKYRVK"
     gene            complement(18140..18376)
                     /locus_tag="PLS64_00705"
     CDS             complement(18140..18376)
                     /locus_tag="PLS64_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42383.1"
                     /translation="MRDYVIYDMYTDDRMFRYEVYKHPNGMYEVCVQSREAEDFAVNE
                     QYLYCDIPEEVTHFTDTLERAIEIGDCKLCKVSA"
     gene            complement(18429..18830)
                     /locus_tag="PLS64_00710"
     CDS             complement(18429..18830)
                     /locus_tag="PLS64_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL42384.1"
                     /translation="MKVKFFDLNADKPLKYAVICARYNGKWVFCKHKERDTYEIPGGH
                     REDGEDIEATAKRELWEETGAKDFDICPVCIYSFTDYGMLYFAEIRSFEKLPPLEIEK
                     INLFDDIPKNLTYPAIQPYLFERVQEFLETK"
     gene            complement(18842..19432)
                     /gene="lexA"
                     /locus_tag="PLS64_00715"
     CDS             complement(18842..19432)
                     /gene="lexA"
                     /locus_tag="PLS64_00715"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRL42385.1"
                     /translation="MKPLSKSQQKIFDYLKECAGEGRVPSVREICEETGLSSTSTVHH
                     HLKALEEKGLISREHGVNRCIQINGMEKNTDVPVLGRVAAGYPILAVENIECYVPVPD
                     SLKRGRELFALRVQGESMINAGIFPDDILIVHRTPVAENGEIVVALVGDEATVKRFYK
                     ENGHFRLQPENDNFEPIIVDEVALLGKVISLVRYFD"
     gene            19975..20580
                     /locus_tag="PLS64_00720"
     CDS             19975..20580
                     /locus_tag="PLS64_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRL42386.1"
                     /translation="MLENKLGLTSSAELAREEERISKKKAAMLFENHILDNLESGKFS
                     TLQTIHKYLFDEIYDFAGKLRTVNIAKGNFRFAPLMYIESALKNIDEMPQSNFDEIIE
                     KYVEMNIAHPFREGNGRSTRIWLDHMLKTEIGKVIDWSKVDKEDYLLAMERSPIRDIE
                     IKYVLKNALTDEIDSREVYMKGIDHSYYYEGYTAFKADELL"
     gene            complement(20801..21076)
                     /locus_tag="PLS64_00725"
     CDS             complement(20801..21076)
                     /locus_tag="PLS64_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42387.1"
                     /translation="MGEFLQNMYVWLWAALAILMIIVGIKYRHEHQGLLAVLMAVFFI
                     FMTVWYGLRTFAGYPMFEGTLNIIFRCILGGFLVALGLIYWFIRKRK"
     gene            21217..22320
                     /gene="hrcA"
                     /locus_tag="PLS64_00730"
     CDS             21217..22320
                     /gene="hrcA"
                     /locus_tag="PLS64_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRL42388.1"
                     /translation="MSIAISTHLSRVLKQQKPTRNEVNGMELAERKKKILSAVIESYI
                     NTGEPIGSKALIDETGLEVSSATVRNDLADLTNKGYLVQPHTSAGRIPTLQGYRYYID
                     NLMKITPVTQGGREYVESELYKSADSPESILKEASGVISRLTGCTAVTTTPSGEESRI
                     HRIRFVQTGSHTAMAVVIASNGIIKTKLFRCDFLLNPELLTVFDKAFNEIFSGMKLSS
                     VNRPFIQTAAARLGELSMFTPGVLVAIMEACETAKEVSVYLSGVTKPLFMSDVNFLNA
                     RNVMEFLNNRHDLAEMLENLPLDTSVSIGGENSRTELAGSAVISTRYRLDGNPSGVLA
                     VIAPVRTDYARAISILECVSESVSTLIDELIEI"
     gene            22350..22907
                     /locus_tag="PLS64_00735"
     CDS             22350..22907
                     /locus_tag="PLS64_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRL42389.1"
                     /translation="MTKKKSEKTEEKKVSEETVAEETETENTEEPAKDENADKIKALE
                     DELSAQKDKYMRLAAEYDNYRKRTANEKLSIYDDATSKACIELLPVADSVTLALANLK
                     DADPDIIKGIELISNQLAKSFEKLKIESYGKAGDAFDPNLHNAVSKIEDENLGADTIA
                     AVYQTGYKIGDKIIRHAMVQVANCD"
     gene            22979..24835
                     /gene="dnaK"
                     /locus_tag="PLS64_00740"
     CDS             22979..24835
                     /gene="dnaK"
                     /locus_tag="PLS64_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRL42390.1"
                     /translation="MAKTIGIDLGTTNSCVAVIEGGEPVVIANAEGARTTPSVVAFSK
                     DGERMVGQVAKRQAITNPDRTVASIKREMGTAHKVTIDGKSYTPQEISAMILQKLKSD
                     AEAYLGETVTQAVITVPAYFTDAQRQATKDAGKIAGLDVKRIINEPTAAALSYGVDKE
                     KDQKVMVYDLGGGTFDVSIIEMGDGVQEVLATAGNNRLGGDDFDKRIIDWMVASFKTE
                     TGIDLSQDKVAMQRLKEAAEKSKIELSGVTTSNINLPFITADQTGPKHLDLTLTRAKF
                     DELTSDLVEATMGPVRSALQDSGLQIGQIDKVLMVGGSSRIPAVQDAVKKLIGKEPFK
                     GINPDECVAIGAAIQAGVLGGEVEGLLLLDVTPLSLGVETMGGVMTKIIDRNTTIPTK
                     KSQIFSTAADNQTQVEINVLQGEREFARDNKQLGLFALTGIAPAMRGIPQIEVTFDID
                     ANGIVNVSAKDLGTGKEQKITISNSTSMSKEDIDKAVKEAEQFAAEDKKRREAVDAKN
                     EAENIVYQAEKLVSESGDKIPEADKNAINTKVAALKEAISKDDAALIGTAKEDLQKTL
                     NDAAAKLYQQAAPQGGAPDMNGAQPNGGNPTGNNGGDPNVYDADFTDVDDNK"
     gene            24958..26100
                     /gene="dnaJ"
                     /locus_tag="PLS64_00745"
     CDS             24958..26100
                     /gene="dnaJ"
                     /locus_tag="PLS64_00745"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRL42391.1"
                     /translation="MADKRDYYEVLGVQKGASDDEIKKAYRACAKKYHPDLHPGDKEC
                     EEKFKEVNEAYEVLSDSDKKARYDQFGHAGVDPNYGAGAGGAGSPFGQGIDIDDIFSS
                     FFGGFGGRRSNNANAPMRGSDIETTVYISFEEAAKGCKQTINYSCVTTCDDCHGTGAQ
                     PGTSPKTCSSCGGSGRVTINQRTPFGVVQTQRTCDACHGRGKIVDNPCKKCGGSGKQR
                     KNKTVDVTIPAGIKDQQILNVSGRGNSGTNGGPAGDLHVYVNVRPHPVFERRGDDVWC
                     EVPITFTQAALGADVVVPTLDGKVSYTVREGTQPGDVFRLKGKGIQRLGGRGRGDQYV
                     RVTVEVPKNLNGKQKELIKQLDGATGDKNYAKRRNFFDKIKKMFNE"
     gene            26304..27239
                     /gene="prmA"
                     /locus_tag="PLS64_00750"
     CDS             26304..27239
                     /gene="prmA"
                     /locus_tag="PLS64_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRL42392.1"
                     /translation="MESWTEIKISVDAKDIDRASDIANVVVPYGIYIEDYTNLEQEVE
                     DIAHIDLIDEDLLAKDRNKAFVHIYLEPDVSPSEAVAFLSERYNAEGIKHSIELLDCL
                     EEDWRNNWKKYFNPIEVGEKLLIRPSWRDDYDAGNRKVLNIDPGLAFGTGGHETTRLC
                     LEMCEKYLKEGDSVLDVGCGSGILGIATLLLGADRAVGVDIDETAVRTAAENAEINGV
                     ADRFTAICGSFTDKVEGKYDIVLANIVADAIMFLSKGIKDFMKDDAVYIMSGIIDTRA
                     EEVKASVSQYFDIIEEHLDNGWACFAARRAAVPRS"
BASE COUNT         8202 a         5766 c         6091 g         7192 t
ORIGIN      
        1 gcggttattt ctttatattc ttaatgactt cattaaaagc cacgaaaaca attgcaagta
       61 caaataaata tgtaatctct gccattggaa tatacacctc ctttccagag gtgtcgaaac
      121 agccgccgcc gtttccttac aaagtgatta tatcaaatat attgtttatg ttcaagtgct
      181 gattgtatat tacatagcat ctgtaaattt gcaatattat tctaataaag gcaatcctac
      241 tttaccgtaa gattgccttt attttatgct ttgtgatttt ctcttaaatc tgttctcttg
      301 atttttccgc tgattgtttt tggaaattca tcgacaattt caaggtgctg aatccacttg
      361 tagcttgcca tacgcttgtt gcagaaggtt ttgattttgt tttcaagctc cttatcatgc
      421 ggtgcgccgt ctgtcatttt gacaatcgcc ttaattgcct gacctctgtc tttgtccgga
      481 acacctataa ccgcacattc aagcacattc ggatattgca taagaacatt ttcaacttca
      541 aacggaccta ctctgaagcc tcttgttttg ataaggtcat cggctctgcc gacataccag
      601 aaatatccgt tctcgtcttt atatgccgtg tcgcccgtgt gatatacacc gtgtcgccac
      661 accttttcat aaagattttc gttgttatag tatgtgatga aaattccgtg ctgttttctg
      721 tcctttggag ggaatacaac aacttcgccg acctcaccct gcttaacctc attgcattca
      781 tcgttaacaa ttcgtacatc ataaagcgga gtaggcttgc ccattgagcc tgcaatcgcc
      841 ttgtcacctt tgagatttgc aagcataaga accgactcgg tttgaccgaa gccctcgcaa
      901 agttcaattc ccgtctgctt aaacacctct tcaaaaactt cattgttgag tgcctcgcct
      961 gctgtcgtaa ggtgagaaag tgcagaaagg tcgtacttgt tcatacctct tttaatgaag
     1021 tatctgtaaa ctgtcggcgg tgcgcagaag gttgtaacct tgtacttttc cattatacgc
     1081 aacagcttgc ccggagagaa tttgtcaaag tcatagacca taacagctga accgcagagc
     1141 cattgaccgt agattttgcc ccatgacgct tttgcccagc ctgtttcggc aacggtgagg
     1201 tgaagtccgc cgtcttttac acattgccag tattttgccg tgataatgtg tccgagtgtg
     1261 tagatatgat tgtgttcaac agccttagga tagcctgttg tgcctgaggt aaagtaaatt
     1321 accataggct cttctgcttt tgtttcaact ctttcaaggg tatcgggagc gttttcaact
     1381 tcttcggtaa aatcaaccgc accgaaaatt tgctttctcg gaacaaaaac ggtttcaagc
     1441 gtgttcggct gagatgcaac cgcaagaata tcttcggcgg tagtttcatc gggagtgcag
     1501 acagctgcct ttatccctgc cgtgttaaca cgatacgaaa tgtcgctttg agtcagaaga
     1561 tttgttgcgg gaataataac cgcaccaagc ttgtggagtg caggggcaac ataccagtat
     1621 tcgtagtttc gtttcagaat aacaagaacc ttgtcgcctt ttttaatgcc gtgatttctg
     1681 aaaacatttg cagccttgtt gctcagtttg ctgatgtcgg agtatgtgaa aatcttttcc
     1741 tctttttcgg gatttgtcca cacaactgcc gtgtcattcg gcacaagttc tgccattttg
     1801 tcaacaacat catagccaaa attatagttt tcgggaaaca caagttcaaa gcttgtcagt
     1861 tttccgtttt cgtcggtagt ttctttacag aaatttttgt ataactgcat tttttacatc
     1921 cttatttaag tcgttttttg gtttgccttt tcaattaata ttgtataaca caaacccttt
     1981 tctatacaat accgcccatt atgaaaaaac acggactaaa atttataatt attcatccac
     2041 ggaatgtagc tatccaaatt tgcataacac tatccgcata tcggtatatt gtgaataaca
     2101 ggagcaaatt attaacattt tcaacacatt tattcacata tcgtttcacg gagttgaaag
     2161 ccgtaaatct gaatattata cagtttttaa acggcaaaac agtatgatga taccctgttc
     2221 tacaaatttg cttttttgct tgcaatcgga taaataaaat attataatat aggttacagt
     2281 cttgtttcaa aaaggtaaca gactgacaga gttaaagaga ataaagaaat aaaaatagaa
     2341 acaaaacatt tcagagggag aaatatatat gagaatagtt gttcttgcag gcggactgag
     2401 cacagagcgt gaagtttcaa tttcatcagg cacacttgtt gccgaggctc tcagaagcaa
     2461 gggacacgag gttgttttgc ttgatgtatt tatgggctat gaggaaaaca tctgtgacat
     2521 tgacgcactt ttcaagcaga actacagctt taccgacgga aattcaatcg gtgacgatgt
     2581 tgaaaataca atcacaaata tcaaagaggc aaaggaaaat cgccttgaca aaacatcacg
     2641 ctatatcggc agaaatgtaa ttgaaatctg cgccgaggca gacatcacct tccttgcact
     2701 tcacggcggt gagggtgaaa acggtcagtt acaggcaacg cttgatttgt tcggaatcaa
     2761 gtacacaggt tcgggatatc tcggttctgc gcttgcaatg aacaaaggtc ttacaaagag
     2821 cgtgtttatg cagaagaata tcaacactcc gtcaggtgaa ctttacaaga acgaaaagga
     2881 cgctcttgca tggaatatgt tcccgtgcat cgttaagcca tgctcgggcg gttcaagcgt
     2941 gggtgtttca aaggttgaga atgcagatca gtataaaaag gctgttaaag aggctctcaa
     3001 atatgaggac gaaattgttg ttgagcagtt cgtaacaggc agagaatttt ccgtcggtct
     3061 tatcggcggc aaggcacttc cgccgattga gattgctcct aagtcgggaa tgtacgatta
     3121 tgccgcaaaa tatcaggcag gcgcaacagt tgaaacctgt ccggcagaca ttgacgaaga
     3181 aaccgacaaa aagctccgtg atgccgcaac agaggcatac gaggcacttc accttgaaag
     3241 ctatgcacga atcgacttcc ttcttgacaa gggcggtttt acatactgcc tcgaggcaaa
     3301 cactctcccc ggcatgactc ccacaagcct tcttcctcag gaggcggctg ttgagggtat
     3361 aagctatccc gatttgtgcg aaaagattat tgaaatttca cttgcaaaat atcccaaaga
     3421 aaaatctgat ttttaataag aggttagtat gaaagacttt actcttgccg aaatcgcaaa
     3481 ggcttgcaac ggcgaatatg tcggcgacga atcgcttaaa aacacaaaaa taacctctgt
     3541 tgagcgtgac agccgacagg tgaagaacgg aagtctgttt cttgccataa agggcgagcg
     3601 tgttgacggt catgatttta tcgaaaaatg ttttgcacag ggcgctgtgt gtgcattgtg
     3661 cgagaaagct cccgaaaatg cgacaaagcc ttgcattgtc gttccatcaa cacttgatgc
     3721 cgttaagaaa atcggcaggg catacagaga aaaattcgac attcccgtta tcggaatttc
     3781 gggcagcgtc ggaaaaacct ctacaaaaga aatgctctat gccgttcttt cacagaagtt
     3841 caagacccac aaaacacaag gcaatctcaa taacgagctc ggtgttccgc taacgcttct
     3901 ttcaatgccc gaggataccg aggcggctgt tatagaaatg ggtatttccg attttggtga
     3961 aatgacaaga ctttccgaaa tggttcagcc cacaatctgc gtgcttacaa tcatcggcga
     4021 atgtcatctt gaaaatctcg gcgaccgtga cggtgttctc aaggctaaaa cagaaatgtt
     4081 caaaaatgcc cgtgaaaatg cagatttcat tctcaacggc gatgacgata agctttcaac
     4141 cgttaagacc gtaaacggca aaaagcctgt tttcttcgga cttgacgcaa agaacgattt
     4201 ttacgcaaag aatataaaaa acagcggtga cggcaaggtt cttgcaacac tctgttttga
     4261 caataatgat attgatgttg aaattcccac gattggtaca tatatggtga caaatgctct
     4321 tgcggctgct gccgcaggca agcttctcgg acttaccgat gaagaaattg caaacggtgt
     4381 cgcctcgtac aaaacagtag gcagtcgtgc caatgttatc aatacaggca aaatcaaaat
     4441 cattgacgat tgctacaacg caaatccgac atccgtcaga gcatctcttg acacactttc
     4501 caaccttgac ggcagaaagg ttgcaatcct cggcgatatg aaggagctcg gaagtgagga
     4561 gttgaaactc cactttgaca cgggtgttta tgcaaaatca aaggatatcg gtacggttat
     4621 aaccgtaggc gagcttgcaa aggagcttgc aaagggtgca gacggcaaag cgtttgacac
     4681 aatcgaagat gcaaaacccg aaattcttaa aacaattaaa agcggagatg ttgtgcttgt
     4741 taaggcatca cattcaatgc agtttgaaaa cattgttgag tttctcaaac agaatttttg
     4801 atttaccgca aaaaaatgtt gacaaatcaa aaatacagtc atataattgt cttataaaga
     4861 aaaaacctgt gagcaagaat agtaggtttt tccaaagctt tcagagagtc agcggttggt
     4921 gtaagctgac agcagaggac ttgccgaatg gacttgtgag ggcgggccga aagccttttg
     4981 gcaagtagtt tccgacggag ctcaaccgtt aaagtgagag gcatatgtta gtatgctgtt
     5041 aaagcgggcg tttatcgtca atttgggtgg taccgcagaa gttgattttt caagactttt
     5101 gtcccttgtg gggacaaagg tcttttttgt ttatgtttaa aacttttacc aaagaaaggt
     5161 tggtgtacga tgattaaacc gtcttacgat gaggtgttga acctcaagga caattacagc
     5221 gttattccga tttataagga aatctacgct gacgcattta ccccgattaa cctgctcaga
     5281 aaaattgcag gaaaatccga caaattcttt cttcttgaaa gtatcgaggg cggtgaaaag
     5341 tgggcaaggt attcattcat cggattcaac cccaaggcaa ggctttcgta caaaaacgga
     5401 actctcacgg ttacgggcga gggtgcaaga gttgttaaaa cagcaaagcc gtatgacgct
     5461 ctccgtgagt atcttgccga ttacaaaact ccgcatttca aagacattcc gccgttcaca
     5521 ggcggtcttg tcggctattt cggttacgca atgatttctg ttgcagagcc tgttttaaag
     5581 ctcaaaagag gagatacaaa cgattttgat atgatgcttt tcgacaagat tatagcctac
     5641 gatcatctta aagaaaaact cattatcatt gttaatatga aaacaaacga caccaaggcg
     5701 cagtatgaac ttgccaaaaa ggatattgca gagataattt cgacaatcac atctcccgaa
     5761 ccccttccaa agcttgagag tgacgggaat gttgaattta caagcacata ttcaaaagaa
     5821 gaattctgca aaatggttga gaaaaccaag gagtacatct ttgacggcga catcttccaa
     5881 tgtgttgttt caagaagatt tgaggcggat tacaaaaatt ccctcatcaa tgcatacaga
     5941 gtgctgagaa gtacaaaccc gtcaccttat atggtttata tgtcaattga cggtgacgag
     6001 attataagca cctctcccga aacacttgta aagcttcaga acggagtgct taacacattc
     6061 ccgattgcag gttctcgccc gagaggtaaa acagatacag aggacaatgc ccttgcagac
     6121 gagcttattc acgatgaaaa ggagcttgca gagcataata tgctcgttga cctcggacga
     6181 aacgacatcg gaaaaatctc cgaatttaat tcggttaagg ttacaaaata tcaggaggtt
     6241 ctccgctatt caaagataat gcacatttgc agtgaggttg agggcaagct caaggacgga
     6301 cttgacgcct ttgacgcaat cgaatccctc ctccctgccg gcacactttc gggtgctccg
     6361 aaaatcagag cctgtgaaat cattgaagag cttgaaagaa ctccccgagg tgtatacggc
     6421 ggagcgctcg gatatgttga tttcaacggc aacctcgaca cctgtatcgc tatcagaatg
     6481 gcggtcaaga aaaatgacaa ggtttatgtt caggcaggcg caggcattgt agccgacagc
     6541 gttcccgaaa gcgaatacga ggaaacctac aacaaggctc ttgctgttat gaatgcagtt
     6601 aaaaatgcag gggaggtaaa cgctttatga tactttttat tgataactac gacagcttta
     6661 cctacaatct tgtacagttc tgcggcagcg taaatcccga tatcaaggtt gtaagaaacg
     6721 atgaaataac agttgacgaa atcaaaaagc ttgctccctc ccacatcatc ctttcgcccg
     6781 gtccgggtta tccgaaagat gccggaatat gtgaggaggt tataaaggag cttgcaggac
     6841 agtttccgat tctcggtatc tgcctcggtc atcaggcaat atgcgaggtt tacggtgcaa
     6901 caatcgccca cgcaataaag cttatgcacg gcaaaaagag cgatatcatt gttgacacgg
     6961 acaagaaaat ttttaacgga cttccaaagg ttgttgaggg tgcaaggtat cattctctga
     7021 ttgcgaaaag agatacggtt cccgacacac tcgaggtaat cgcagaagac cgtgacggcg
     7081 aggttatggg agttaagcac aaggattttg agctttacgg attgcagttc caccccgaat
     7141 caattctcac ctcacacggt atggaaatga taaagaattt tattacactt tgcaaataac
     7201 acggaaagga ttgaagtttt atgattaaag aggcaataaa aaagttggtt gcaggcaatg
     7261 acctcacttt tgatgaggcg gcacaggtta tggacgaaat gttctcaggc acagcaacgc
     7321 agtcgcagat ggcggcatat ctcacagcct tgagaatcaa gggtgaaaca attgacgaaa
     7381 ttaccgcaag cgcacaggtt atgagagaaa aagcactcca catcaagcct aaccgtgatg
     7441 tactcgatat cgtcggcaca ggcggcgacg gtacaggcac attcaatatt tcaacaacag
     7501 cggcatttat aattgccgcc gcaggaatcc ccgttgcaaa gcacggcaac cgttcaatgt
     7561 caagcaaaag cggttcggca gactgtcttg aacagctcgg aatcaacatc aacatcactc
     7621 ccgaaaagag cgaagaggtt cttaacaaag caggcatctg ctttatgttt gcacagggct
     7681 accattcatc aatgaagtat gtaggtcctg tccgtaagga aatcggaatc aggaacattt
     7741 tcaatgttct cggacctctc acaaaccctg caggcgcaga ccttcaggta acgggtgtgt
     7801 attctgaggc tcttgttgag ccgattgcac aggttttctc aaacctcggt gttaagaaag
     7861 gctatgtttt ctacggcatg gacggcatgg acgaggttac tctcacaaca acaacaaagg
     7921 tgtgtgagat tgacaacggc aagttcaaca cattcattct caatcccgaa gattacggtc
     7981 ttaaactttg tgcacccgag gatcttgcag gcggtgacgg caaagaaaat gctgaaatca
     8041 caaaagaaat tctcagcggt gaaattaagg acgcaaagcg tgatatcgtt gttctcaatg
     8101 ccgcactcgg actttgcaca ggcggcaagg cagattcaat tcaggacggc ataaagcttg
     8161 caaacgaaat tatcgacagc ggcaaggctt atgcaaagat tgaagaattt gcaaaggctt
     8221 ccaacgaata aaggtgacac aatgatactt gaaacaattg cagatgcaaa cagagtaaga
     8281 tatgaagaaa tcgaaaagca agttccgctt gaagtgataa agcaaaaagc actttcaatg
     8341 gaaacagaca atgaatttca gtttgaaaag gcgcttgcgg gcaaggacat ttcattcatt
     8401 tgcgaggtca aaaaagcgtc cccgtcaaag ggaattatcg cagaggattt tccctatgtt
     8461 cagattgcca aggattatga aaatgcaggc acaagtgcaa tttccgttct gacagagccg
     8521 aagtggttca aaggcgaaaa tgcttttctt gaagaaattt caaagaatgt aaaaattccg
     8581 cttttgagaa aagattttac cgtgtgtgaa tatcagattt atgaggcaaa gacaatcggt
     8641 gcgtcagccg tactgcttat ctgctcgctt cttgacacgg acacaatcag aagatggatt
     8701 aagctttgcg actctctcgg actttcggca cttgttgagg ctcacacagc cgatgaggtg
     8761 tattccgcaa ttgccgcagg agcaagagtt atcggtgtaa acaaccgaaa tcttcgtgac
     8821 ttcacggtgg acataaataa ctgtacaaag ctccgtaagc ttgttcccga caacattatt
     8881 tttgttgccg aaagcggaat taaaacacgg gatgacatca aggtgcttga aaacgcagga
     8941 gtaaacgcag ttctcatagg tgaaaccctt atgagaaatc ccgacaaaaa agccgcactt
     9001 gacgaattgt cgggcagaaa ataaaaaatt gatttataag ccgtgtgcaa agcacaccaa
     9061 tgatataaaa gtaattaagg ggaatcaaga tgacaaaagg aaaattcggt attcacggcg
     9121 gacagttcgt tcccgaaacg ctgatgaatg caattaatga atttgaagag gcatacaaca
     9181 ggtacaagga cgatcccgag tttgttgcag aactcgacac acttatgcgt gaatacgcag
     9241 gcagaccgtc acttctctat tatgccgaaa aaatgacaaa agacctcggc ggtgcaaaga
     9301 tttatcttaa gcgtgaggac ttgaaccaca caggttcaca caagctcaat aactgcctcg
     9361 gtcagtgcct tcttgcaaag agaatgggca aaacaagagt tattgccgaa acaggcgcag
     9421 gtcagcacgg tgttgcaacg gcaaccgtag ccgcactttt aggacttgaa tgtgaaatct
     9481 ttatgggcaa agaggacaca atccgtcagg ctctcaatgt ttacagaatg aaacttctcg
     9541 gtgcaaaggt caatgccgtt gaaacaggca cgatgactct taaagatgcc gtaaacgagg
     9601 caatgcgtga atggacaaac agggtttccg acacacacta tgttctcggc tcggtaatgg
     9661 gaccgcaccc gttcccgact gttgtccgtg attttcagag tgtaatcggc aaggaaatca
     9721 agtcacagat gcttgaaaag gaaggcagac ttcccgatgt tgttatggct tgcgtaggcg
     9781 gcggaagcaa cgcaatgggc gctttctatg agtttatcaa ggatgaaaat gtaaagctta
     9841 tcggctgtga ggcagcaggc ctcggcacag atactccaaa aactgccgct acaatcgcaa
     9901 caggcacact cggcatcttc cacggcatga agtcgtactt ctgtcagaat gaatacggtc
     9961 agattgctcc cgtatattca atttcggcag gtcttgacta ccccggtatc ggtcccgaac
    10021 acgcaaacct tgccgacacg ggcagagcaa gctatgttcc cgttacagac gaagaggctg
    10081 taaacgcatt tgaatatctt tcaagaacag agggtatcat ccccgcaatt gaaagcgcac
    10141 acgcagttgc acattgtata aaaattgccc cgacaatgga caaagacaag attatcgtag
    10201 tcaacctttc gggcagaggc gacaaggatg ttgcggctat cgcaagatac agaggggagg
    10261 atctccatga ctgatattaa atctgcattc caaaacggca aggcgttcat cccgtttgta
    10321 acagcgggtg atcccgatct tgaaacaaca gaaaagcttc ttattgaaat gagcaaaaac
    10381 ggtgccgata tcatcgaaat cggtattccg ttttcagacc cgattgccga gggtgttgta
    10441 attcaggagg ctgacctccg ttctctttcg gcaggcacaa caacagataa aatctttgat
    10501 atggtaaagc gtatccgtcc gcagattgac gccgttcttg ccgttatgac ctatatgaat
    10561 ccgattttcg tatatggtac ggaaaggttt atggcaaagt gtcaggagtg cggaatctcc
    10621 gcagttatcg ttcccgacac accgtatgaa gaaaagcacg agcttaccga ttacagcgga
    10681 aaatacggca ttgatgtaat ttctctcatc gctcctacat ctcacgagag aatcaagatg
    10741 attgcaaaag aggcagaggg ctttgtttac tgcgtttcat caatgggcgt tacaggcgta
    10801 agaagtgaaa tcacaaccga tgtaggcgaa atggtaagac ttgttaagtc agtaaaggat
    10861 attccgtgtg caatcggttt cggcatttca actccggagc aggcaaagaa aatgagcgaa
    10921 agtgcagacg gtgttatcgt tggttctgca atcgtgaaaa ttattgcaaa gtacggcaaa
    10981 gacagcgtta agcctgtttg cgactatgtt aaaagtatga aggaagcgat ttcgtgagta
    11041 cactcgaaga acttcaaagc gtaattgaca aaagcaaaag aatagtattt ttcggcggtg
    11101 cgggagtttc gacagaaagc ggaattcccg acttccgaag tgttgacggt ctgtacaatc
    11161 aaaagtacga ttatccgccc gagcagattc tcagccacac attttttgtt cacaaaacaa
    11221 gcgagttctt ccgcttttat cgtgacaaaa tgctatgcct tgacaaaaag ccgaacaagg
    11281 cgcactataa gcttgcagag cttgaaaagg cgggaaagct ttcggctgtt gtcacccaga
    11341 acattgacgg acttcatcag gcggcaggca gtaagcgtgt ttatgagctg cacggaagtg
    11401 ttcttcgcaa ctactgcaca aagtgcggca tgttctatga cgcagagttt atcaagaact
    11461 ctgccgatat tcccaaatgt acttgcggag gtatcataaa acccgatgtt gtgctttatg
    11521 aagagggact tgacgacagc acggtaacgg gtgcggtcaa cgcaatttca aaggctgaca
    11581 cgctgattat tgccggcaca agcctcacgg tctatccggc ggccgggttt atccgttatt
    11641 tcggcggcga taatcttgtg cttatcaacc gtgatcccac cccaatggac aagaacgcaa
    11701 atcttgtgtt ccatgaaaaa gtgggagaat tgcttgacaa aattaccgta aacatataaa
    11761 atttatgcga caggaaaatt tctgttattt ttctgttaca gcatatagat aaatgaaaaa
    11821 atcaatacac aagatgttgt tgcggaacag agaaaaccga taaaatccgc ataaaatcca
    11881 actttttttg taaaaagact tgatttttgc acattcttgt gctatcataa ttcctatatg
    11941 accgatactt taaggaggtg ggacaatgcc aaacatcaaa tctgctaaaa agcgtgttaa
    12001 agttattgct acaaagactg ctcgtaacaa ggctacaaaa tctgctctca gaacagctgt
    12061 aaagaaggct tattacgcag ttgacactaa cgctgacaac aaaacagagg cagttcgtct
    12121 tgctattaag aaaattgacc aggcagcagc taagggtatt cttcacaaga acactgcagc
    12181 aagaagcaaa tcatcacttg ctaaaagact taacgcatca gcataagtgc aataaatctt
    12241 acaggcttaa aagcagagaa tattctctgc ttttttattt tgttctgttg acatttttgt
    12301 aaaaactgtt agaatataaa taaagatacg gcagatacta ctgccataca agcatacaaa
    12361 atttggaggc gacagaatat gaaaaatcat aagtgcagat gggctttaat tatcggtatc
    12421 atctctgcag ttgcagctct ttctgctgct ctcacagcgt tctttattgt taaggataag
    12481 cagaaaaaag atgacgagga gcttatggaa tacctcgaca gctcaattga gtaattattg
    12541 agtaaatctt aaaagccgaa tgcggaccgt tcattttttg ggcggtccgt ttttatgaaa
    12601 cttcatttta ttttcgtggt gattttatga tacgggaaga acttgaccga cttctctgtg
    12661 acgccaaaag caacgcagaa ctcaaagaaa aacttctgaa aaccgaacag agcgaaaatc
    12721 cgattgataa cttctgttcc ctctgccggt cgcatgggta caaaatcagt gcaggcgaac
    12781 tttttgcact cggacttgac gaaagcgaca ccaagcttcg cagtgtgaac ggcggcggag
    12841 tgaacgcaat tgacggctgg gacgatgcct acgagcaatt tattcttaca ttgaaatgga
    12901 catgactatg aaacactact tcccgaaata ctgcgaaaaa ttcagatgca ttgcgaacct
    12961 ctgtcccgac agctgttgca gggactggga tgttgttgtc gatgatacaa gctttgattt
    13021 ttataataca gtcaaaggcg agtttggcga caggctcagg tcgcttatga caattgacga
    13081 ggacggcgac agaattttca tcaggcaagg tgaaaaatgc ccgttttgga acagcgatat
    13141 gctgtgcgac atttacatca acctcggtga aaatcacctt tgtcacacct gtaaggaatt
    13201 tccgagaatc actcaggact acaccgtttt ttgtgaacat acgctctcgt ttgcctgtcc
    13261 cgaggcggca aggcttatgc ttgaatcgga ttttgacaca ggctttgcaa ccgatattcc
    13321 gcaggactta cagactgatt acagcacaac cgagatgaat tttctgctct ctgcaagaaa
    13381 acgcactttt gaaattctct cagactgcaa aatgtccttg tccgaaagat tgtgcagact
    13441 tcttgccttc aacgagcgag tgcagaatat gcttgacgat gaaatttttg atatcacggc
    13501 tatgccgaca gatgaatata aaaagcaaag ctgcggagat gcgtcattca tttttgattt
    13561 gcacaaaagc cttgatatta tgagtaatga ttggctatgt gagcttgaag caaccgccga
    13621 ttttgcaaaa aataatacgc tgtcaagccg ttttgacaaa ccgcttaccg cctatcttga
    13681 ctactatgtt cgcagatatt acctttctgc aatcgacagt tttaatgtca tatccacaat
    13741 caagcgaatg gtctgtgcat acatagtaac cgcttatgaa ctcgaaaggc ccaacaatcc
    13801 gactcaaacc gaaattgtga aaattctgca gggatattca aaagaggttg aacattccta
    13861 tgaaaacggc gaactcctcg aagatgaatt tatcttcaat ccactttttt caaccgacaa
    13921 tttaataggt attttataaa taaatcccga acacatttta ctgtgttcgg gatttattgt
    13981 ttattcttta ttttcgtccg aattcttttt gtttcgtttt ttcacggaaa caatgtcaag
    14041 ttcaaaccag aatgtactgc ctttgccgag tgtactgcga acgccgtatc ttgctttgtg
    14101 gctgtcaaga atacccttga ctattgacaa gccgagtccc gtgccgataa cgccacgctt
    14161 atgctccttg tcaaccttgt agtaacgatc ccagatgtat tcaagctttt cgggaggaat
    14221 gccgtcaccg tgatccgtta cctcaatgag aacctttttg ccgtttacct tttgcgttac
    14281 aataacagtt ttgtcttcac cgacataatt gactgcattg ttcacaagat tatagattac
    14341 ctgtgaaatt ttcagttcat cggcaaaaat gtaaacatct tcgtcactct caaacagaat
    14401 gttgtatccg tcctgttcct tgagctttgc gtaccttgtg aaaatattct tgatgctgtc
    14461 ggtaagacag aactccgatt tttcgggttg aattgaaccc gattgaatct ttgagaggtc
    14521 aagcaaatcg ttgacaagtg ttgaaagtct tgttgcctcg tcaataataa tctgaatatt
    14581 ttcgggagtg ttttcaccgg gcaggtcacg cataacctcg ccgtagcccg taatcattgt
    14641 cagcggtgtt ctcaaatcgt gtgagatgtt ggcaataagt tctttttgca acttttcggt
    14701 tgcggcaagc tccgtttttg cgtagttcag cgtttcgttg agttcctcaa cctcaagata
    14761 tcctcttgcg ttaaagtcaa catcatagtt tttctttgca agctcctttg ccgccgtgtt
    14821 tgtcttagaa atcggtttcg caattctgta gcttgcgtaa agcgaaatta taacggcaag
    14881 aagcacaaac acaaccgaaa cgattgcaag ctggtcacgg acgatttcaa ccgtgttagt
    14941 cagcggtgtg attgacgatg ttacaatcaa aaagcattcg ctgccgtcct gcagattgat
    15001 aatgtcggtg taaaccatat tttgaacatt tttatcggga gcggaagcat accgaatagt
    15061 attcctctcg gctgtgtcgg atgaagaata ttcattaaaa aactgtcgct caaacttgcc
    15121 tttaatgctg tcctctttgt agttacttgt gatgaacata taatgtccgt cattttcctc
    15181 tgccagcttg tagtatgaat agacatcgtg atactgaaaa ttcaggtttg aaacggggtt
    15241 gtcatattcg caacaatact tcaatgtgaa aagagaagtt gaatcgtaaa catataccga
    15301 aagtgaattg tagcttgcaa cattttcaat tgttccgaga gcgtttttgt tttcttcaat
    15361 cgactttgaa ataacctgtg cgttcttttc aacctgtgaa gattttgttg ctgtgtagaa
    15421 cgattcaaga aaaaaggtct ggaacaccca caacacgatc acaattgcaa atccgaacag
    15481 aagaaaatag cataacagct taaaattcaa cggcagataa tggcggtcaa atcgtttgtt
    15541 agattgtttc aaaacggtaa cccacccctc gaagcgttac aatgcatctg ctgtattcgc
    15601 caaggctctt tctgagaagc ttaatgtgtg tgtcaagcgt tctttcgtca ccgaagaagt
    15661 cgtagcccca gacctcgctt ataagctttt cacgggtgag tgcaagtcct ttgttcttta
    15721 ccatatagaa aaagagttcg tattcctttg gcgtcatctc aattcttttg ccgtcaattg
    15781 taacaattct ggctgagaaa tcaaccgaaa gaccttcgta tgtaaataca tccttcttca
    15841 cattctcttc gttctgagcg ccttgtgaac gcttgattac ggcattaaca cgcatcataa
    15901 gctcctttgg cgagaacggc ttaacaacat agtcgtcact gccaagctca aagccgtgaa
    15961 ttttatcgta ctcctcgcct cttgccgaaa gcataatgat aggtgttttc ttgaattttc
    16021 tgatttccct gcaagcggaa aagccgtcaa gctcaggcat cataacatcc attatgataa
    16081 ggtcaaagtc ctctttacgg caaatttcaa ttgcctgcat accgtcaacg gcttcttcaa
    16141 cctcaaagcc ctcaaattcg gcatattttc tgataatctg tctgattctt gcctcatcgt
    16201 caacaacgag tatcctgctc atttttgtac gcccctttta ttaaaattat aagcgtcggt
    16261 taagacgcag tgtattccga ctgttactct atcatccgaa tgtgataaaa ttatgttaat
    16321 tttcaatttt gttggtgaag tatcgcacgg cgagccgccg taccccattc gtgtagatag
    16381 tttgcagtat taaaaacata ttatgcccga gcgtaatatc gtaattcttc gtaccacact
    16441 gttggggtga acctcgttcg ccgctgtaca tcaaatcaca actgttataa caatccgtta
    16501 aaatgattat accacatttc atttcttatt gaaataccgt tgaaaataaa ttataatgta
    16561 tagtgttaaa atttggtgct aaattcatat taaggagttc ggatatggaa ataagaaaaa
    16621 tcaaggcgca gacggtatgc gatacggtta aaaagctgtt taccgactgc aactatttta
    16681 tcggcaaaga cattatgtgc gcccttgaaa ccgcaaggga taacgaaagc tcgcctgtag
    16741 gcaaaagtgt actttcacag attatcgaaa acgataaaat tgcggctcgt gaagaagttc
    16801 cgctttgtca ggatacgggt atggctgtcc tttttgttga atacggtgac agggttgtta
    16861 ttgaggacgg cagttttgat gaggctgtca acgagggcgt tcgcagagcc tatattgacg
    16921 gctaccttcg caagagtgta gtaaatgatc ctgttttcga cagaatcaat acaaaggata
    16981 acactcctgc aattatccac acaaaaatcg tcagcggaaa tcagattaaa attaccgccg
    17041 gcggcaaggg cttcggcagt gaaaatatgt cgcagataaa aatgctcacc ccgtcaaagg
    17101 gcattgaggg cgttaagcag tttattcttg atactgtttt tcaggcaggt ccaaacccct
    17161 gtcctcctat ggttgtcggt gtcggaatcg gcggtacttt tgaaagagcc gcccagcttg
    17221 caaaaaaagc aaccttccgt ccgattgatt ccaaaaatga agatgaacgc tatgcacagc
    17281 ttgaggacga gctccttacc gaaatcaata aaatgggatt cggtcctgca ggacttggcg
    17341 gcaatacaac cgcaatcggc gtgaatattg aaacttcacc cacacacatt gcaggaatgc
    17401 ccgttgcggt caacatctgt tgccatgccg caagacacgc aagtgcaacg atctgaggag
    17461 gaaaagctgt ggaaaagaaa attacacttc cgattacgga cgaagatatc aaaaatctta
    17521 aagcaggcga cagcgttctg ctctcgggtt caatcatcac aggcagagat gccgcacaca
    17581 aaagacttta tgaattgatt cagaagggcg aaaaacttcc cgttgacatc aaaggcgagc
    17641 ttatctatta tgttggtcct gctcctgcaa agcccggtta tgcagtaggt cctgcaggtc
    17701 cgacatcaag ctatcgtatg gataagtacg ctccgtcact gcttgacctc ggactcaaag
    17761 gaatgatagg caaaggcgca agaaataaag aagttgtcga tgcgattgtt cgcaacaagg
    17821 gcgtgtatct tgtcgcaatc ggcggtgcgg cggcgcttat cgccaagagc atcaagagag
    17881 aggagattct ttgctatgaa gatttaggca cagaggctgt tcgcagatat gaggttgaag
    17941 atttcccgtg catagttgca atcgacagca acggcaacaa tgtctatgaa accgaaccgc
    18001 caaaatacag ggtgaaataa tcggcaatat aaaattttat ataaataaaa acggtcgggc
    18061 gacaatgctt gcatttggca aactgtcatc cgaccgtgaa ttttattaaa ctgtttctgc
    18121 aaagacaggc ggctgttaat taagcactta ctttgcacag cttgcaatcg ccgatttcta
    18181 tagctctttc gagggtgtcg gtaaaatgtg tgacttcttc gggaatatcg cagtagagat
    18241 actgctcgtt cacggcaaaa tcttccgcct ctctcgactg aacgcacact tcatacattc
    18301 cgttcgggtg cttatagacc tcgtaacgaa acattctgtc atctgtgtac atatcgtaaa
    18361 tcacatagtc acgcatttta tcacttcctc tcaatatatt tttgttgcgg attgcaaaca
    18421 gtacttggtt acttcgtttc taaaaactcc tgtactctct caaaaaggta cggctgaatt
    18481 gcaggatatg tcaaattttt tggtatatcg tcaaaaagat taatcttttc aatttcaagt
    18541 ggcggcagtt tttcaaaact gcgaatttcc gcaaaataga gcattccgta atccgtgaac
    18601 gaatagatgc acacgggaca gatgtcgaaa tcttttgcac ccgtttcttc ccaaagttca
    18661 cgctttgcgg ttgcttcaat atcttcgccg tcctcacgat gaccgcccgg aatttcatat
    18721 gtatcacgct ctttgtgctt gcagaaaacc cattttccgt tgtatctcgc acaaataacg
    18781 gcatatttca acggcttgtc tgcgttgagg tcgaaaaatt taactttcat agctaatcct
    18841 tttaatcaaa atatcttaca agcgagatca ccttgccgag caaagcgact tcgtcaacta
    18901 taatcggttc aaaattatcg ttttcaggct gaagtctgaa gtgaccgttt tctttgtaaa
    18961 aacgctttac ggtcgcctcg tcaccgacaa gagcaacaac gatttcgccg ttctccgcaa
    19021 caggtgtgcg gtgaacaatt aaaatatcgt cagggaaaat gcctgcgtta atcatacttt
    19081 cgccctgaac acgcagagca aaaagctctc tgcccctttt taagctgtcg ggaacaggca
    19141 cataacattc aatattctca acggcaagaa tcggataacc tgccgcaact ctgcccaaaa
    19201 caggaacatc ggtgtttttc tccatgccgt taatttgaat acaacggttc acgccgtgtt
    19261 ctcgtgaaat aagacctttt tcttcaagtg ccttgaggtg gtggtgaacg gttgatgttg
    19321 aactcagtcc cgtttcttcg cagatttcac gaactgacgg aactctgccc tcgccggcac
    19381 attcctttag ataatcgaaa attttctgtt ggcttttgct taaaggtttc atatcgcaac
    19441 gccccttgtt tgtaattaca gtatgattgt accatatatt ttcggaaaaa tcaaacattt
    19501 gttttatatt tttaaaaaaa ttttttattt cgtcgaataa ataaagaaat tatttgagtt
    19561 ttgattgatt aacagaaaaa acagatgctt ttcattccga tttcaaaaag ggttcacaca
    19621 gttttcatat ttgcattgtt taatataagc gtactcagaa agacggaacc gcatccgtca
    19681 gagtataatt gttctaccct cctcaatgcg agtctgaaca aagactcgta attgtacccc
    19741 tcatttttta aagaacataa gaaagcaccg ggtcgccgca agcccggtgc tttcgtcgtt
    19801 taaggacaaa ttaattgcaa cactgcagcg gtgaacaatt gcaatgccac gctcgggcat
    19861 aatagttttt taaatattgc aaactatcta cacgaactaa aataaacata aactaaaaaa
    19921 ttgaattatc atctgcacta tgatacaata tatatagtaa agtgaggtgc ttgtatgctt
    19981 gagaacaaac tcggattgac ttcttctgcg gagcttgctc gtgaagagga gcgaatcagt
    20041 aagaaaaagg cggcaatgct ttttgaaaat cacattcttg acaaccttga aagcggaaag
    20101 ttttcaacat tacagacaat ccacaagtac ctttttgacg aaatctacga ttttgcggga
    20161 aagttgagaa ccgtaaacat tgcaaagggc aatttcagat ttgcaccttt gatgtacatt
    20221 gaatcagctt tgaaaaatat tgatgaaatg ccgcagagca attttgatga gattatagaa
    20281 aagtatgtcg aaatgaacat tgcgcaccca ttcagagagg gcaacggtcg cagtacacga
    20341 atctggcttg accatatgtt gaaaacggaa atcggcaagg ttatcgactg gagcaaggtt
    20401 gacaaggaag attatctgct tgcaatggag cgcagtccca tacgggacat cgagataaaa
    20461 tatgttttga aaaacgcatt gaccgatgaa attgacagcc gtgaggtgta catgaaaggc
    20521 attgaccaca gctattatta tgagggctac acagcgttta aagcggacga acttttgtaa
    20581 attcaaaatt gaaaattgac ggcgagccgc cgttctcagt aaaatgcccg aatgaaaatg
    20641 tttattgaaa attgtgcaga caataacaat gccacgctcg ggcataattg gtttttaaac
    20701 attgcaaact atctacacga acgaaaaaat atgaacataa aaataatgga cagcagaaat
    20761 aaaatctgct gtccgatttg ttttttatac atatatatag ttattttctt tttcttataa
    20821 accaatatat caaaccaagt gcaacgagga aaccgccgag gatgcaacgg aagatgatat
    20881 taagcgtacc ctcaaacatt gggtagcctg caaaggttcg cagtccgtac catacggtca
    20941 tgaagataaa aaacacagcc attaaaactg caagcaagcc ctgatgttcg tggcgatatt
    21001 tgattcctac gataatcatt aatattgcaa gagccgccca aagccataca tacatattct
    21061 gaagaaattc gcccataaaa ttcaccctta aacattactg ataagtagat tatagagtat
    21121 cctcggcaca atgtcaaacc gtgttatgtg aagattttct gaaaacgaaa aattttttca
    21181 aaaacgcttg acttttactg actttggtat tatattatga gtatagcaat tagcactcac
    21241 ctatcgagag tgctaaaaca gcaaaaaccg acacgaaatg aggtgaacgg tatggagctg
    21301 gctgaaagaa aaaagaaaat attatcggca gtaattgaaa gctatatcaa cacaggcgag
    21361 ccgattggtt caaaagcgtt gattgacgaa acgggacttg aggtttcatc ggcaacggtg
    21421 cgaaatgacc ttgcagacct tacaaataaa ggctacttgg ttcagccgca cacctcggca
    21481 ggacgaattc caacgctgca gggctacaga tactacatcg acaatctgat gaaaattacc
    21541 cccgtaaccc aaggcggacg ggaatatgtt gaatccgagc tttacaaaag tgcagattcc
    21601 cccgagagta ttctcaaaga ggcaagcggt gttatttcaa ggctgacagg ctgcacggcg
    21661 gtaacaacaa caccttcggg tgaggaaagc agaatccaca gaataagatt tgttcagacg
    21721 ggttcgcaca cggcaatggc tgttgtgatt gcctcaaacg gaatcataaa aacaaagctg
    21781 tttcgttgcg atttcctttt gaatcccgaa cttttgaccg tgtttgacaa ggcatttaac
    21841 gaaatctttt cgggaatgaa gctttcatcg gtcaaccgac cgttcataca gacagcggcg
    21901 gcaaggctcg gtgaactttc aatgtttacc ccgggagtgc ttgtggcaat tatggaggct
    21961 tgtgaaacgg caaaggaagt cagcgtttat ctcagcggtg tgacaaagcc gttgtttatg
    22021 tccgatgtca attttcttaa cgcacgaaat gttatggagt ttttgaacaa cagacacgac
    22081 cttgcggaaa tgcttgaaaa tcttccgctt gatacctccg tcagcatagg cggcgaaaac
    22141 agcagaacag agcttgcagg gagtgcggta atctccacac gataccgact tgacggcaat
    22201 ccgtcaggtg tgcttgcggt gatagcaccc gtgagaacgg actacgcaag agcaatttca
    22261 attcttgaat gtgtgtccga aagcgttagc actcttattg atgagctgat agaaatatga
    22321 taaataaaaa tccttgaagg aggataaaga tgactaagaa aaaatccgag aaaaccgaag
    22381 aaaaaaaggt ttccgaagaa acagtcgcag aagaaactga gactgaaaat accgaagagc
    22441 ctgcaaagga tgaaaatgcg gataaaatca aggctcttga agatgagctt tcggcacaga
    22501 aggataagta tatgcgactt gccgccgagt acgacaatta ccgtaaaaga acggcaaacg
    22561 aaaagctttc aatctatgac gatgcaactt caaaggcttg tatcgagctt cttcccgttg
    22621 cagacagcgt aacgcttgct cttgcaaatc tgaaagatgc cgatcccgat atcatcaagg
    22681 gtattgagct tatttcaaat cagcttgcaa agagctttga aaagctaaag attgagtcct
    22741 acggcaaagc aggcgatgca ttcgatccga acttgcacaa tgcggtttca aagattgagg
    22801 acgaaaacct cggtgccgac acaatcgcag ccgtttatca gacgggttac aaaatcggtg
    22861 acaaaattat ccgtcacgca atggtacagg ttgccaactg tgactgattt catatataaa
    22921 tattcagcag aaaacacaca aacaaaaata attaaaaatt caattggagg aatttatcat
    22981 ggctaagaca ataggtatag atttaggtac aacaaactca tgcgtagcag ttatcgaagg
    23041 cggcgagcct gtagttatcg ctaacgcaga aggcgcaaga acaactccgt ctgttgttgc
    23101 tttttcaaaa gacggcgaaa gaatggttgg tcaggttgcc aagcgtcagg ctatcacaaa
    23161 ccctgacaga acagttgcat caatcaagag agaaatgggt actgctcaca aggtaacaat
    23221 tgacggcaag agctacactc cacaggagat ttcagcaatg attcttcaga agctcaagtc
    23281 agatgcagag gcttacctcg gtgaaacagt tacacaggca gttatcacag ttcctgcata
    23341 cttcacagac gcacagcgtc aggctacaaa ggatgccggt aagattgcag gtcttgatgt
    23401 taagagaatc atcaacgagc ctacagcagc cgctctttca tacggtgtag ataaggaaaa
    23461 agaccagaag gttatggtat atgacctcgg cggcggtaca ttcgatgtat ctatcatcga
    23521 aatgggcgac ggtgttcagg aagtacttgc aacagcaggt aacaaccgtc tcggcggtga
    23581 tgacttcgat aagagaatca tcgactggat ggttgcatca ttcaagacag aaacaggcat
    23641 tgacctttca caggataagg ttgcaatgca gagattaaaa gaggctgccg agaagtcaaa
    23701 gattgagctt tcaggtgtta caacttctaa catcaacctc ccgtttatca cagcagacca
    23761 gacaggtcct aagcaccttg accttactct tacaagagca aagtttgacg aactcacatc
    23821 agacctcgta gaagctacaa tgggtcctgt aagatcggct cttcaggatt caggtcttca
    23881 gatcggtcag attgacaagg ttcttatggt tggcggttct tccagaatcc ctgctgttca
    23941 ggacgctgtt aagaagctta tcggcaaaga gccgttcaag ggcatcaacc ctgatgaatg
    24001 tgttgctatc ggcgcagcta ttcaggcagg cgtactcggc ggtgaagttg aaggacttct
    24061 cctcctcgat gtaactccgc tttcactcgg tgttgaaaca atgggcggtg ttatgacaaa
    24121 gattatcgac agaaacacaa caatcccaac aaagaagagc cagattttct caactgccgc
    24181 tgataatcag acacaggttg aaatcaatgt tcttcagggt gagcgtgaat ttgcccgtga
    24241 taacaagcag ctcggtcttt tcgctctcac aggcattgct cctgcaatgc gtggtattcc
    24301 gcagattgaa gtaacatttg atattgatgc caacggtatc gttaatgtat ctgcaaaaga
    24361 cctcggtaca ggcaaagagc agaagattac aatcagtaac tcaacttcaa tgagcaagga
    24421 agatatcgac aaggctgtta aggaagctga acagtttgcc gcagaggaca aaaagcgccg
    24481 tgaggcagtt gacgctaaga acgaagctga aaacattgtt tatcaggcag aaaagctcgt
    24541 cagcgaaagc ggtgacaaga ttcccgaggc tgacaagaac gcaatcaaca caaaggttgc
    24601 agctcttaaa gaggctatct caaaggacga tgccgcactc atcggcacag ctaaggaaga
    24661 cctccagaag actcttaacg atgctgccgc taagctttat cagcaggctg ctccgcaggg
    24721 cggtgctccc gatatgaacg gtgctcagcc aaacggcggt aatcctacag gcaacaacgg
    24781 cggagatcct aatgtatatg acgctgattt caccgatgtt gacgataaca agtaatttat
    24841 gttagaataa catatagtca aaggagctgt tatattgcgg ctccttttgg ctgtataaga
    24901 cgataaccgg cttgcaatat tgcgtgattt cggtattttg acaaaggtga gagtaatatg
    24961 gcagacaaaa gagactacta cgaggtgctt ggcgttcaaa agggcgcaag cgacgatgaa
    25021 ataaaaaagg catacagagc ctgtgccaaa aaatatcacc cggatttgca tcccggcgat
    25081 aaagaatgcg aggaaaaatt caaggaagta aacgaggctt atgaggttct ttccgattcc
    25141 gataaaaagg ctcgttacga ccagttcggt cacgcaggcg ttgatcccaa ctacggcgca
    25201 ggtgcaggcg gagcaggaag tccgttcggt cagggtattg atattgacga tatcttctca
    25261 agctttttcg gcggattcgg cggcagacgc tccaacaatg caaacgctcc tatgcgtggc
    25321 agtgatattg aaactacggt ttacatttca tttgaagagg cggcaaaggg ttgcaagcag
    25381 acaatcaact attcatgcgt aacaacctgt gacgactgtc acggaacagg cgctcagccc
    25441 ggcacaagcc ccaagacctg ttcgtcatgc ggcggttcgg gcagggtaac aatcaaccag
    25501 agaacacctt tcggtgttgt ccagactcag cgtacctgtg atgcctgtca cggcagaggc
    25561 aagattgttg acaatccttg taaaaagtgc ggcggttcgg gcaaacagcg caagaacaag
    25621 actgttgatg taacaattcc ggcaggtatc aaggatcagc agattcttaa tgtaagcggc
    25681 agaggtaact caggtacaaa cggcggtcct gcaggcgacc ttcatgttta tgtaaatgtt
    25741 cgtccgcacc ctgtttttga acgcagaggt gacgatgtgt ggtgcgaagt tccgataacc
    25801 tttacacagg cggctctcgg agcagatgtt gtcgttccta ctcttgacgg caaggtttcc
    25861 tacacggttc gtgagggtac acagccgggt gatgtgttca gactcaaggg caagggtatt
    25921 cagcgactcg gcggcagagg cagaggcgac cagtatgtaa gagttaccgt tgaagttccg
    25981 aagaatctta acggcaagca gaaagagctt atcaaacagc ttgacggcgc aacaggcgac
    26041 aagaactacg caaaacgcag aaacttcttt gataaaatca agaagatgtt caacgagtaa
    26101 cacagaataa ataagatttg tggggcattt gtccccacaa attttttata cggcgtgccg
    26161 ccgttcccaa ttcgagcaga taatttgtaa tgaaaaaaac atattatgcc cgagcgtaca
    26221 attgtgattg tctgaacaat ttatccgcca ctacattaca aacaaaaata tcggcataaa
    26281 atgcccgtga atcagaggtt aaaatggaat cctggacaga aataaaaata tccgttgacg
    26341 caaaggatat tgacagagca agtgatattg caaatgtcgt agttccctac ggcatctata
    26401 ttgaagatta cacaaacctt gagcaagagg ttgaggacat tgcccatatt gaccttattg
    26461 acgaggattt gcttgcaaag gacagaaaca aggcttttgt tcacatttat cttgaacccg
    26521 atgtttcgcc gtcagaggcg gttgcattcc tttcggaacg ctacaatgcg gagggtataa
    26581 agcattcaat tgaacttctt gactgccttg aagaggattg gagaaacaac tggaagaaat
    26641 atttcaaccc gattgaagtc ggtgaaaaac ttcttatccg tccgagctgg cgagatgatt
    26701 acgatgccgg caaccgcaaa gttctcaaca ttgatccggg ccttgcgttc ggcacgggcg
    26761 gtcatgaaac cacaaggctt tgccttgaaa tgtgcgaaaa gtaccttaaa gagggcgatt
    26821 ctgttcttga tgtgggttgc ggcagcggta ttctcggtat tgcaacattg cttttgggtg
    26881 cagaccgtgc cgtcggtgtt gacattgacg aaactgcggt gagaactgcg gctgaaaacg
    26941 cagaaattaa cggcgttgct gaccgcttta cagctatctg cggcagcttt accgacaagg
    27001 ttgagggcaa gtatgacatt gttcttgcca atattgttgc cgatgcgatt atgtttctct
    27061 caaagggcat taaggacttt atgaaggacg atgccgttta cattatgagc ggtatcatcg
    27121 acactcgtgc cgaagaagtc aaggcatccg taagtcagta ttttgacatc atcgaagaac
    27181 atcttgacaa cggatgggct tgttttgccg cacggcgtgc cgccgttccc cgttcgtgaa
    27241 gatagtatga t
//
LOCUS       DAOMVE010000006        26331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_279_length_26331_cov_2.947443, whole genome shotgun sequence.
ACCESSION   DAOMVE010000006 DAOMVE010000000
VERSION     DAOMVE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 26331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26331
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_279_length_26331_cov_2.947443"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PLS64_00755"
     CDS             <1..1269
                     /locus_tag="PLS64_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRL42393.1"
                     /translation="FNPYAVADIGMLLSFSATTGIILWSRPIERSVLRFFHYKNKRKD
                     GFVGTVVYYIKRLFKICVNMLSVSVSAFLWILPITAVAFNRISPTVILVSLLCEPFVS
                     ALLVCSLLCSVLFICPFISFFAYPFGLVSGLCSKAILWLVSTFSQLPFSTIKTHSLYW
                     FVWIGVSVLLAIGGLFVINRKFYVKISVSVSISVLVIGASLNVLLTADNGEMKIYNVG
                     NGVFATVNCPDSCSVISCGGNRASADDVTADISERYSDIEYMIIPNQNNKYSSLERFA
                     VTKFDLNNILVYDNDIKKQNLLNAFDGNSRQTFGGNSHFTLNLSDTVTDEVICVDNTM
                     FQFIKGKNMTVLFVPTDADLSNLPEKYRNPDCLLIDSVPENFDLISCNTVIFSGSEKQ
                     FKKNYDSIKEISPTVISTSERNITVNLNGG"
     gene            1271..2314
                     /gene="holA"
                     /locus_tag="PLS64_00760"
     CDS             1271..2314
                     /gene="holA"
                     /locus_tag="PLS64_00760"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRL42394.1"
                     /translation="MPRITEADLKKQIKNKSFSPVYLIYGSEQMFVKSYTKKLCEAVA
                     GKNPSDFNYHTFSGDIDFQELAASLQIMPFMAERNCVVVTDIYFDNMVKDRLDTLKEL
                     TSRAWDGTVLIISMPTYVPSKNKTSFTALIKRVEKIGSVCCFEKINSQIIEKYVAKWA
                     NENGKLISHLNASKIISNVGDDLNLLKNEVNKIAAYAKGEEITDRDIDLLSTVNLEAR
                     TYDMADDVINGRGDRAFRKLDTLFCQREEPINILYALSSAYVDAYRMRVADECSVSKE
                     TVAKDFAYKNRAFVLNRARTSVSRTSTEALRKSLDVLVETDEKLKSVSVNQRFLIEQL
                     ISRLLLIAKEGRI"
     gene            2314..2892
                     /locus_tag="PLS64_00765"
     CDS             2314..2892
                     /locus_tag="PLS64_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4093 domain-containing protein"
                     /protein_id="HRL42395.1"
                     /translation="MLKIREAVIVEGRYDKIKLSSIVDAPIIETNGFRVFSDKEKQSL
                     IRKIAETRGILIMTDSDGAGFVIRNFLKGSVDNSKIKNCYIPQIKGKEKRKAKGGKEG
                     LLGVEGVSDEVIINAIRKSGAEIIGEENNIPERKITKADLFVLGLTGTENSEINRKKI
                     LKKLGLPSYLSTNAFLTAINCLYSFEELKEML"
     gene            2960..3352
                     /locus_tag="PLS64_00770"
     CDS             2960..3352
                     /locus_tag="PLS64_00770"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42396.1"
                     /translation="MKKSLRLRNDCILFSIGGAGYALLEILWRRKTHWSMAVTGGTAF
                     VSLFRFYKRFSKMNMLQKCISGSAIITALEYCCGCVVNLKFRLNVWDYSNCRLNLKGQ
                     ICPFYSTLWAFLCIPVSALCTSLCKKIQ"
     gene            complement(3399..4697)
                     /locus_tag="PLS64_00775"
     CDS             complement(3399..4697)
                     /locus_tag="PLS64_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRL42397.1"
                     /translation="MTSDIIMGVVILVLILLSAFFSAIETAFSFVNKVRVQRYKDDGN
                     KKAAAALYIIEHFDNALTTILICNNVVNLSCSSIATVLCMNLFGDAGSAIATGATTFL
                     VLTFGEIVPKCLAKEHCDAFSLKTAGLLRGLMTLLTPLVWIFTRFKMIALKIAGSSGD
                     APSVTENELKYIVESIEEEGVLEESESEMVRSALDFDETTAEEILTPRVDITFISVDD
                     SPEKIKNIIIENRYSRIPVYEGTVDHVVGILHTRDYLERLADGKAPDVKELMQPPYFV
                     FKTQQLSKILNAFKRTKIHLAVVTDEYGGTLGIVTMEDLLEEIVGEIWDEDEEIEHNY
                     YKIGKGEFLVNGDMELEDMLGLFDMDEDSLECDSVTVGGYILEHAGTIPHKRDNIEAD
                     GFKFTIMEVKDQRILRVVVKKNDTSEENESDEKSENKKSE"
     gene            4951..5760
                     /locus_tag="PLS64_00780"
     CDS             4951..5760
                     /locus_tag="PLS64_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRL42398.1"
                     /translation="MPQKSKSSKVSSKKGNYVKNPAPDDDPQKLANDSPISEEQTDRI
                     GETGSVLTTHKDCVLHCLTIIGQIEGHYILSQQNKTTKYEHVIPLLVSVEEDERIDGL
                     LVLINTVGGDVEAGLAIAEVISGMKKPTVSIVLGGGHSIGIPLAVAAKKSFIAKSASM
                     TVHPVRTTGLTLGVPQTFEYFQRMQDRITTFVAENSNITKDRYNQLVLNTGELVMDIG
                     TILEGEEAVEEGLIDEVGTVSDAIDALYDLIKENKESKPKSAKSRSKKQEK"
     gene            5904..6863
                     /locus_tag="PLS64_00785"
     CDS             5904..6863
                     /locus_tag="PLS64_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRL42399.1"
                     /translation="MSILDAIIQGVVQGLTEFLPVSSSGHLAITQHILGAGTGESNLF
                     FNVMLHVGTLVAVVAFYHKLVWDLIKEFFLMLKDIFTGKFKWSKMNYNRNLVVMLIIG
                     LIPLFLLFLPIPGTDMKLKDLADRLSASPVLIVTACSLILTSVLLTIGIIRNRKSVAS
                     THKHLATKSNAKATRDSFNVVDAILVGVTQVCAALLPGLSRSGSTLSVGEMRGISKQK
                     ALDYTFVLGIPSILAAALLEGVDAIKSPEGLSNIEFLPLIIGMAVSAVVGYLAIVIFK
                     WFLKSDKMEIFVIYTAIVGVAFIVISIIEMNTGVNLFTGAPIA"
     gene            6885..9641
                     /locus_tag="PLS64_00790"
     CDS             6885..9641
                     /locus_tag="PLS64_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRL42400.1"
                     /translation="MAANKNKADKTKNTASGRKKTSSKTSSKGAKTASRTKASAVPKR
                     SSGLSPRVRAILYAAVAAVFVCIIFIKGSNVWTMIRSFFFGVLGFGIFLVPVTLFYLC
                     VMTEKEKQVARYKLKLFLCVVIILFIGALIYAGSGAKYSDMNFFACLGNLFKDASDTS
                     RYMTFDCGIIGGLLGYPLVVLFGSTAALFTSFVVTAALILIVANISIKDMTAAASRTA
                     VHIREAGEERARVRRERREENRERKLAERGDIDIALEGENRKSSGSFIDIPLDDKKTK
                     VKKAKSAVNGIDTDIADSKEENAADKDLSQDLINIINRASKPMGKSADNTVSAIAEDI
                     SNEKSASKAEQTEVKPDAETENIVKKALLKSKKDNSEEAGDEIAEQLDRAVRSDNSQK
                     EEKKYQFPPIQLLKPKLNSDDSDAMEEMQNNAKKLIDTLTSFGVKASIVNICRGPSVT
                     RYELQPAPGVKISKITNLSDDIALSLAANGVRIEAPIPGKAAVGIEVPNKVVSMVTMR
                     ELIDSDKFRNSKSKLTTVLGRDISGEIVVTDLAKMPHLLIAGTTGSGKSVCVNSILMS
                     ILYKATPDEVKLLLIDPKMVEFSKYKGIPHLLIPVVSDAKKAAGALAWAVNEMLQRYK
                     IFSEYDCKDIDSYNSLIEKNMNYMEKNPPVVNEEGEEVQPVLEVNGLPVAKEKMSRVV
                     IAIDELADLMMAAPSEVEDSICRLAQMARAAGMHLILATQRPTVNVITGLIKANVPSR
                     ISLKVSSNTDSRTILDTGGGEKLIGRGDMLFSPVGAPKPIRVQGCYASDEEIEGVTHY
                     IKKAYSAQYNSEIEEKIKRIAAEEIAQGKKSGDSDSSSDEGLDIDSKMEEAIKCVIEA
                     GQASTSLLQRRLKVGYARAGRMIDDMEQMGVVGPHQGSKPRDVLMTYNEWLERRNALE
                     NRAD"
     gene            9662..11575
                     /locus_tag="PLS64_00795"
     CDS             9662..11575
                     /locus_tag="PLS64_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRL42401.1"
                     /translation="MAFLPMNAKEMRDRGWSEVDFVYVCGDSYVDHPSFGAAIITRVL
                     EDCGYKTAFLAQPNWKNDDDFTQFGKPRLGFMVSAGNIDSMVAHYTVAKRKRHDDAYT
                     AGGKNGRRPDRAVTVYCNIIRKLYPDSPIIIGGLEASLRRFAHYDYWKNEVMPSIIFD
                     SKADLLVYGMGELQTIEIAKRLSEGYPVESLYDIRGVCYKIRTDDYVPKSVVELPSYE
                     RVKEDKRDYAIASRRELEEADAVRGKTLIQRHGKYILVQNPPMQPLDTKQLDYVYSLP
                     YERWYPKCYESLGGVPGINEVLFSITHNRGCFGACNFCSLAFHQGRAVTVRSEKSIIE
                     EAESFLDNPRFKGYISDVGGPTANFRLPSCEKQKKLGLCKNRRCLAPTPCPNMQVSHT
                     EYLDILRKLRNLKGIKKVFIRSGIRFDYLIEDENDEFFRELVKYHISGQLRVAPEHCS
                     AAVLDKMGKPHIESYKRFCKMFYDFTGKENKDQYVVPYLMSSHPGSTLSDAVELALFC
                     KRENIHPKQVQDFYPTPGTISTSMFYTELDPYTLKEVYVPKSENEKAMQRALLQYFIP
                     ENKPLVIKALIKAGRRDLIGNDKKCLVTPMAGQTQGGYSKQNNNRNSQNQKGRGKNGK
                     DKFAKYRRKNKKK"
     gene            11706..12467
                     /locus_tag="PLS64_00800"
     CDS             11706..12467
                     /locus_tag="PLS64_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL42402.1"
                     /translation="MESDFSVSFINVGSADCTLIKCGDKSVLIDGGTNLVTDKITAYL
                     KRSSVTHLDAVIVSHPDSDHIGSLPDIIDEFGTDVVYFGKYSDSHKTPEYEKLVNSIK
                     ENNIKTVIPVSDKPVEIGNMTFKFYQPEKNFGNTNDNSLVTMLEYDEKRFLFTGDISS
                     KREESMVNSDKELKCDVLKVAHHGSKYSSSEDFLAKASPETAVISVSADDTALPDYYI
                     TALLNSVCKNVYRTDSDKTVMITVENGEICTKTHA"
     gene            12541..13767
                     /gene="tyrS"
                     /locus_tag="PLS64_00805"
     CDS             12541..13767
                     /gene="tyrS"
                     /locus_tag="PLS64_00805"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRL42403.1"
                     /translation="MGVYEELVARGLIAQVTDEEEIRELVNSGKATFYIGFDPTADSL
                     HVGHFMALCLMKRLQMAGNKPIALVGGGTGMIGDPSGRTDMRQMMTKETIQHNVDCFK
                     KQMSRFIDFSEGKALLVNNADWLLDLNYVELLRDVGACFSVNNMLRAECYKQRMEKGL
                     SFLEFNYMIMQSYDFYALYQKYGCNMQFGGDDQWSNMLGGTELIRRKLGKNAYAMTIN
                     LLLNSEGKKMGKTQSGAVWLSAEKTSPYDFYQYWRNVDDGDVIKCLKMLTFLPLEEID
                     ELAKLEGSEINKAKEILAHELTELVHGKEEADKAQEAARAIFSNKTNTDNMPSTTLTE
                     ADFADGEIGILDLMKKCGLIPSNGEGRRLIQQGGVSVDDEKVTDVYAKVAKSDFEKGY
                     VVIKKGKKVYHKAILG"
     gene            13801..14238
                     /gene="mscL"
                     /locus_tag="PLS64_00810"
     CDS             13801..14238
                     /gene="mscL"
                     /locus_tag="PLS64_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRL42404.1"
                     /translation="MKKFFSEFKEFIMRGNVLDLAVGVIIGAAFQAIVTSLTSDIISP
                     ILGIFGGVNFDQFQVTINGATIMYGKFITAVINFLIMAFVIFLIVKLVNKVMSLGKKT
                     EPEEAPTTKKCPFCFSDIDIKATKCPHCTGDIIDLTEKTDDKK"
     gene            complement(14378..14683)
                     /locus_tag="PLS64_00815"
     CDS             complement(14378..14683)
                     /locus_tag="PLS64_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRL42405.1"
                     /translation="MGITLLRIIIKEERPYVRYGVAPVFAKNSKPDSMPSRHTASAFI
                     IAMAILRFNVWAGIAYLFIAVMISVSRVCAGVHYVRDVIVGAAIAILCGIVFLFLLF"
     gene            complement(14923..16755)
                     /gene="asnB"
                     /locus_tag="PLS64_00820"
     CDS             complement(14923..16755)
                     /gene="asnB"
                     /locus_tag="PLS64_00820"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRL42406.1"
                     /translation="MCGIAGWIDHSQDMSERIDLLNRMSETLDRRGPDENGLYINRGA
                     ALMHRRLVVIDRENGKQPMSVRHKDTTYVIVYNGELYNTAELREELKASGFHFRGHSD
                     TEVVLKSYIKYGVDCCKKLNGIFAFAIYNTSDKSLFLCRDRIGVKPLFYYEYNGGLLF
                     ASEIKALLASKVVKPQIDEQGLNEIFFLGPARTPSFGVFKGVFELNPGECAMYRHSKL
                     RRKKYYTVEAKEHTDNEVTTIEKTRYLLTDAIQRQLVSDVPLCFFLSGGLDSSIIVKT
                     ASMYNKEHKLGKINTYSVEYRDNEKYFQKSNFQPTPDYEFISMMSKNADTKHREIVLD
                     NTLVCDALYESVQARDLPGYVDVDSSLLLFCKEIKQNYTVALSGECADELFGGYPWYH
                     NHEILFEDCFPWSKSQDIRRSILKDGVLKNGEEYVRQRYLDTISLAPKLKSDTDLDRR
                     MREMFYLNFYWFMQCLLERKDRCSMFSGLEVRVPFCDYRLVEYAYNMPWELKAWGNRE
                     KGIVRKAFEDILPEEICWRKKSPYPKTHNPIYFNECVKRVRKILKKRSILNELLDRKG
                     IEDIIENPDKITNPWYGQLMKAPQILAYIIELDYWFDKYNVEIV"
     gene            complement(16769..17839)
                     /locus_tag="PLS64_00825"
     CDS             complement(16769..17839)
                     /locus_tag="PLS64_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRL42407.1"
                     /translation="MFLSKSAVKAVNFFKAIGIFLLCITALLFSKECSKGAENGIGLC
                     LSTLVPSLFPFMVLASYITDSGLAEKIGRHLSWLTKPLFGLDGCFASAIILSLVGGYP
                     VGAKTVNSLYKKGAASESECKRAGLFLVCSGPGFLVNFIGAQLYSSIEVGLIIFAAQC
                     ISVFILGFALKFVCRNKLDDNSNLETLISTPQKGDALVKSVLDGARGMFAICAFVVLF
                     SAFTEIFCSHITDENIQKPFLILTEVCNAVTAVSKDLPIEVVAFSAGFGGLCVHFQIF
                     SALGDIKANKLLFFFCRILQGFITALLTHLGIKLFSVTTDVFSTSTVENFSFFGGTAL
                     SGAALLFTALCFLYSLKNYKQN"
     gene            17913..18185
                     /locus_tag="PLS64_00830"
     CDS             17913..18185
                     /locus_tag="PLS64_00830"
                     /inference="COORDINATES: protein motif:HMM:NF019488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HRL42408.1"
                     /translation="MPKKKRIADKNERLPSPMFDMGTMPYIEMFGNRRITVEGSTGIL
                     LYDSESIKINTCKTVISFCGRGMTLKCISSSCVEIEGFVTDINFLS"
     gene            18195..19373
                     /locus_tag="PLS64_00835"
     CDS             18195..19373
                     /locus_tag="PLS64_00835"
                     /inference="COORDINATES: protein motif:HMM:NF018591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HRL42409.1"
                     /translation="MLLKIIRFLRGSVDFTASGKFPERFMNITAKNGVNLWNPVPKKN
                     AISASMYLSDYKKIRILARKSRVKTRITAKHGLPFIINRYKKRTGLFAGAVCGIILCI
                     FLSNFIWSVKISGTEEISNTYLESLLGDNGISVGVWKNNLDTDQIERNIQLKCDDIRW
                     MSINITGSLVTVEVKETYKKPKLDTSKNPCNVKAIKDGVITRIQAYNGRPVVTKGSGV
                     VNGQILVSGLDETKQGDMRYLRANAKVYADITEQQTLSIPKKVTYSYADENYVDRKNF
                     RFLWLDFPCNLAFENYDNFVSTVHGENLFINDVVLPVGLKIRTDRELVTTENSVSKNK
                     AEEIAKADCILYEIFTKNKSKVISKQISVKENKGSYRVTTDYIFNENIADQVDFDVTE
                     "
     gene            19455..20228
                     /locus_tag="PLS64_00840"
     CDS             19455..20228
                     /locus_tag="PLS64_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRL42410.1"
                     /translation="MRILCIGDVVGSIGCEFLRNNLPTLKKVKGVDFVICNGENSADG
                     NGLTPVSAKHLFDSGVDAITLGNHSFRRKEVYDCLDSNPFIVRPANFPENSTPGKGII
                     NVDTGRRIVSIINIMGNSYMDNNLNCAFECVDKMIKQAESKIIIVDFHAEATGEKRAM
                     GYYLDGRVSAMFGTHTHVQTSDAQVLPKGTGYITDTGMTGTIHSVLGVKSEIIIAKLK
                     NKLPQRFDLASGECKMECTLFDVDDKSGKCVSAESMRIE"
     gene            20450..22096
                     /locus_tag="PLS64_00845"
     CDS             20450..22096
                     /locus_tag="PLS64_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRL42411.1"
                     /translation="MINGQILKQAVISGANNISNQKNSINDLNIFPVPDGDTGTNMSM
                     TVNAAVAALEDFDGTCAGDAAGITASAMLRGARGNSGVITSLIFRGFAQGLKDIEEVD
                     GKNLAAALGIGVDAAYKAVMKPTEGTILTVARMAYEAGIEAAKYDRDVVSVWQTIVDK
                     ANEALAITPQLLPVLKKAGVVDAGGKGLCVIFEGMLSVIKNGIMIEADDDTAPATVST
                     FESAAAEFDDDIRFTYCTEFIVGRNAEIETEIGELRSYLESIGDCVVVVNDDEIIKVH
                     VHTEQPGNALSKGLEFGQLLTVKVENMKEQHKNVKSTKKKAEKEKFVPAEPENDFGFV
                     AVAAGNGLKDLFKDLGCDNVVSGGQSMNPSTDDIYEAIMATPAKNVLVLPNNKNIILA
                     AEQTIPMVKDRNVIIVPTRTIPQGMTAMLNFDPEISAESNAQLMTDALASVGTGLVTF
                     AARSSEFGGKKIKEGDIIALENGRLTITEKSTVKALVKLAKDMVSRDTSFITLIYGED
                     VSEEEAEKAYGELREKFGSRTDITLVKGDQPVYYFILSVE"
     gene            22171..>23364
                     /locus_tag="PLS64_00850"
     CDS             22171..>23364
                     /locus_tag="PLS64_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRL42412.1"
                     /translation="MTSLYKTPVTNLNGIGEKRAALFARLGIKSVGDLINFYPRTYED
                     WSNIKHIDELEYGETVCIKAVVATSVNDTRISGGRIISKTAVYDETGSIQLVFFNNKY
                     ISSMLRQGGEYFFYGKISSDSYGMQIVSPTFAPAVKGTQIRPIYRQTAGLPSKSVESA
                     VRQALSMLPSKIKDPLPDAIRERFDLCSLRQALFDIHFPKNRQQLEAARKRLVTEELV
                     VLNLGMRNLRDHSRGVTSLEITKDYSKEFESLLPFTMTNAQKRAVSDCINDIINKSSP
                     LNRLVQGDVGSGKTAVAASVCYTVAKNGFQTAFMAPTEILARQHYKTFQKLFENTDIK
                     VGLLTGTLRESEKKQIRKSLADGSIDMVIGTHALITDKTEFKNLALAVTDEQHVAFLA
                     DHLGRG"
     assembly_gap    23365..23374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23375..24267
                     /locus_tag="PLS64_00855"
     CDS             <23375..24267
                     /locus_tag="PLS64_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRL42413.1"
                     /translation="QHRFGVAQRAKLLGKGNNPHLLVMSATPIPRTLGLIIFGDLDIS
                     IIDELPPSRKPVKTVLRTSAMRGGVYDFIRSEVKHGRQAYIVCPLVEEGELDDVASAE
                     EYAADLMLREFPDIAVGIVHGQMKSDEKDEVMRKFVENEYSVLVATTVIEVGVDVPNA
                     TVIVIENAERFGLSQLHQLRGRVGRGSSQSYCILISDKGSKTTRERLEVMCSTNNGFV
                     IADEDLKMRGPGDFFGHRQHGLPQMSIADFADTKSLELSQKIADCIMDRYGDIRNDEI
                     RLLKAEVDRLFERGGQNALN"
     gene            24295..>25623
                     /locus_tag="PLS64_00860"
     CDS             24295..>25623
                     /locus_tag="PLS64_00860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRL42414.1"
                     /translation="MRKTITKTLSLLCMLCIIICSAFTTAGAASYPNDVKTESDSILL
                     VNMDSGQTVYEKDADSKRYPASTTKIMTYIIAVENIADLDNTKIPIKQSVLDVLKNTG
                     SSLANVENHVGKSMTAIDLLYSMMVPSGNDAAMVLADYIGEGNVDNFVKLMNEKAKEL
                     GCENTHFANPDGLHDPDHYTTARDMYKITTYALTLPRFEEITNTCTYTCDGDDTALVT
                     TNYLIDANRGGEYYYMYAKGIKTGTTNEAGRCLVTTASADGYSYMAILLHAPYEEGVT
                     EDYATMTDAADLFRWALTSLEMNTVATSSKPLCEVKVNLAWGKNSTLLYPETDLSAIV
                     PKDCTDENIIIEQDVPESVDAPLDKGSVVGKATIYYKADANSEKQKLAEVNVVAGEKI
                     ERSGILYVFNIIGTVFQSYWFIVIIALIILVLIIYLIISKIHGKRKRKKRN"
     assembly_gap    25624..25633
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25737..26195
                     /gene="nrdR"
                     /locus_tag="PLS64_00865"
     CDS             25737..26195
                     /gene="nrdR"
                     /locus_tag="PLS64_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRL42415.1"
                     /translation="MKCPYCSYEESKVIDSRSADDGERIRRRRECLRCGKRFTTHEVI
                     ETVPIIVVKRDKSREVFDRNKLTGGILRACEKRPVSIEQIENMVNDIEAQLQSSLDRE
                     ITSKQIGELAMDQLKNIDEVSYVRFASVYRQFKDINTFMDELNKLLTEKK"
BASE COUNT         8056 a         5469 c         5692 g         7094 t
ORIGIN      
        1 ttcaacccat atgcggtagc cgatatcggt atgttgcttt cattttcggc aacgacgggc
       61 ataattctgt ggtcaagacc gattgaacgc tctgtgctga gattctttca ctacaaaaac
      121 aagcgtaagg acggttttgt cggaacggtt gtctattata tcaaaaggct gtttaaaatc
      181 tgcgtaaata tgctgtcggt ttcagtatct gcatttctgt ggattttgcc gataaccgct
      241 gttgcattca acagaatttc cccgacagtc atactcgttt ctctgttatg tgaaccgttt
      301 gtttcggcac ttttggtgtg ttctctgttg tgttcggtac tttttatttg cccgttcata
      361 tcattttttg catacccgtt tggactcgtt tcgggattat gttcaaaggc aattttgtgg
      421 ttggtttcaa ctttttcaca actcccattc agcacaatta aaacccattc gctctattgg
      481 tttgtgtgga taggtgtcag cgtacttctc gcaatcggcg gattgtttgt aataaatcga
      541 aaattctatg tcaaaatttc tgtttcggta tcaatttcag tcctcgtaat cggagcgtcg
      601 ctcaatgtgc ttttaacagc cgataacggt gaaatgaaaa tctataatgt cggcaacggc
      661 gtttttgcaa cagtcaactg ccccgacagc tgttccgtga tttcatgcgg aggcaacaga
      721 gcaagcgcag acgatgtgac agccgatatc agcgaaagat actcagatat tgaatatatg
      781 ataattccaa atcagaataa taaatactct tctcttgaac gctttgcggt cacaaaattt
      841 gacttaaata atattttggt atatgataat gacattaaaa agcaaaatct tttgaacgct
      901 tttgacggta acagccgaca gactttcggc ggaaacagtc attttacgct gaatctttcc
      961 gatacggtga cggatgaggt aatctgcgtt gacaacacaa tgtttcagtt cataaaaggg
     1021 aaaaatatga ctgtattgtt tgtgccgaca gatgcagacc tttcaaatct gcccgaaaag
     1081 tatcgcaatc ccgattgttt gctgattgat tccgtccccg aaaatttcga tttgatatca
     1141 tgcaataccg tgatattttc gggatcggaa aagcagttta agaaaaatta tgattctatt
     1201 aaagaaatca gtccaaccgt tatttcaaca tctgaaagaa acataacggt caatctgaac
     1261 ggaggataaa atgccgcgca tcaccgaagc agacctgaaa aaacaaatta aaaacaaaag
     1321 cttttcgcct gtgtacctaa tctacggttc ggagcagatg tttgtcaaaa gttacacaaa
     1381 aaagctttgt gaggctgttg caggcaaaaa cccgtccgat ttcaattatc atacattcag
     1441 cggtgacatt gattttcagg aacttgccgc atccctgcaa ataatgccgt ttatggcgga
     1501 acgcaactgt gttgttgtga ccgacattta ctttgataat atggtgaagg atcggcttga
     1561 tactcttaaa gagcttacaa gcagagcatg ggacggtacg gttctcataa tttcaatgcc
     1621 gacttatgtt ccgtcaaaga ataaaacttc ttttaccgct cttataaagc gtgttgaaaa
     1681 aatcggcagc gtctgctgtt ttgaaaaaat caattcacaa attatcgaaa agtatgttgc
     1741 aaagtgggca aacgaaaacg gcaagctgat ttcacacctc aatgcatcaa agattatttc
     1801 aaatgtcggc gatgacctca atcttctgaa aaacgaggtc aacaaaattg ccgcatatgc
     1861 aaagggtgaa gaaattaccg accgtgatat cgacctattg tcaaccgtaa accttgaagc
     1921 aagaacatat gatatggctg acgatgttat taacggcaga ggtgacagag cgttcagaaa
     1981 gctcgacaca cttttctgtc agcgggaaga accgattaac attctctatg cactttcatc
     2041 cgcctatgtc gatgcgtatc gtatgcgtgt tgcagatgaa tgctcagtca gtaaagaaac
     2101 cgtggcgaaa gattttgcct acaaaaatcg tgcgtttgtg ttaaacaggg cgagaacctc
     2161 cgtttcaagg acttcaaccg aggcacttcg gaaaagcctt gatgtgcttg ttgaaacaga
     2221 cgaaaagctg aaatcagttt ccgttaatca aagattttta attgaacagc ttatttcccg
     2281 tttgcttttg attgcaaagg agggcagaat ctaatgctca aaatccgtga ggctgtcata
     2341 gttgagggca gatatgacaa gataaagctt tcatcaattg tagatgctcc gataatcgaa
     2401 accaacggtt tccgtgtttt ttccgacaaa gaaaagcagt cgcttatccg caaaattgcc
     2461 gaaacaaggg gaatacttat aatgaccgac agcgacggtg caggctttgt aataaggaat
     2521 ttccttaaag gctcggtcga taattcaaaa attaaaaatt gctatattcc tcaaatcaaa
     2581 ggcaaggaaa aacgcaaggc aaaaggcggt aaagaggggc ttttgggcgt tgaaggcgtc
     2641 agcgatgagg ttataataaa tgcaatccgc aaaagcggtg ccgagataat cggcgaagaa
     2701 aataatattc ccgaaaggaa aattactaaa gccgatctat ttgtcctcgg acttacggga
     2761 actgaaaatt cagaaataaa ccgcaagaag attcttaaaa aactcggtct gccgtcatat
     2821 ctttcaacaa acgcattttt gaccgcaatt aactgtcttt attcttttga agaactaaaa
     2881 gagatgctct gacaaaataa aaaattcata ggttaaataa atcacctgtt tcggcaaaaa
     2941 ctaaaacagg tgatttctta tgaaaaaaag tttacggttg cgtaacgatt gcattctctt
     3001 ttcaataggc ggagcaggct acgctctgct tgaaattttg tggcggagaa aaactcattg
     3061 gtcaatggcg gtcacgggcg gaactgcgtt tgtgtcgctt ttcagatttt acaagcgctt
     3121 ttcaaaaatg aatatgctgc aaaaatgcat atcgggaagt gcgataatta ccgcacttga
     3181 atactgctgc ggctgtgttg tcaatttgaa attcaggctt aatgtctggg attattcaaa
     3241 ctgccgactt aatttaaaag ggcagatttg ccctttttac agcaccttgt gggcatttct
     3301 ttgtatcccc gtcagcgcat tatgcacttc tctctgcaaa aaaatacagt aaaaaaacag
     3361 gagcggtaag taaaaatact gctcctgtta tgttttattt attcagactt tttgttctcg
     3421 cttttttcat cggattcgtt ttcttcggaa gtgtcgtttt tcttgacaac aacccgtaaa
     3481 atacgctgat ccttaacctc cataatggta aacttaaagc cgtcggcctc aatgttgtca
     3541 cgcttgtggg ggattgttcc tgcgtgttca agaatatatc cgccgaccgt cacgctgtca
     3601 cattcaagac tgtcctcgtc catatcaaac agaccgagca tatcttcaag ctccatatca
     3661 ccgtttacaa ggaactcgcc cttgccgatt ttgtagtagt tatgctcaat ttcctcgtcc
     3721 tcgtcccata tttcgccgac aatttcttca agcaaatcct ccattgtaac aataccgagt
     3781 gttccgccgt attcatccgt aactaccgca agatgaattt ttgtgcgttt gaatgcattc
     3841 aaaatttttg aaagctgttg tgttttgaac acaaagtaag gcggctgcat aagctcctta
     3901 acatcgggcg ctttaccgtc tgcaagtcgt tcaagataat ctcttgtatg gagaatgccg
     3961 acaacatggt caaccgtgcc ttcgtacacg ggaattcttg aatagcggtt ttcaatgatg
     4021 atattcttga ttttttcagg cgagtcatca acgctgataa atgtgatgtc aaccctcgga
     4081 gtgagaattt cctcggcagt agtttcgtca aagtcaagtg cggagcgaac catctcgctt
     4141 tcgctctctt caagaacgcc ttcttcctca atgctctcaa caatgtattt aagctcattt
     4201 tcagtaactg acggagcgtc accgctgctg cctgcaatct ttaaggcaat cattttgaat
     4261 cgtgtgaaaa tccacacaag cggtgtaaga agtgtcataa gtcctctgag caatccggct
     4321 gttttgagtg aaaaggcatc gcaatgttcc tttgcaagac atttcggcac aatctcaccg
     4381 aatgtcagaa caagaaaagt tgttgcaccc gttgcaattg ccgagcctgc gtcaccgaaa
     4441 agattcatac acagcacagt cgcaattgaa gaacagctca ggtttacaac attgttgcag
     4501 atgagaattg ttgtaagggc attgtcgaaa tgctctatta tgtagagcgc tgctgccgct
     4561 tttttgttgc cgtcatcctt gtaacgctga acacgcacct tgtttacaaa cgaaaatgca
     4621 gtttcaatcg ccgaaaagaa cgccgaaagc aaaataagaa ctaaaatgac aacacccata
     4681 attatgtcag atgtcaactg aatatcactc cataacaact tccgacaggt tctgtcggaa
     4741 taatatttct ttaatactac tataattctt ttaaataatt attacttatt agaaataaag
     4801 aaataaaatt attgaaataa ttttgctgta atggtataat aagcactaaa ggctttttac
     4861 aattgaacag tgtggcttgt gcatcttatt ttttggtatg catacatttt gcttgcaatt
     4921 tcggtgaaac tattctcgga ggtgcataca atgccacaaa aatcaaaatc ttcaaaagtc
     4981 tcatcaaaaa aagggaacta tgttaaaaat cctgcgccgg atgacgatcc gcaaaagctt
     5041 gcaaacgaca gccccataag tgaagaacaa acagaccgaa tcggcgaaac aggttctgtt
     5101 ctcacaactc ataaggactg tgtgctgcat tgcctgacga ttatcggtca gattgaaggt
     5161 cattacattc tttcgcaaca aaacaaaacc acaaaatacg agcatgtcat ccctctgctt
     5221 gtttcggttg aggaggacga aagaattgac ggactgcttg tgctgattaa cacagtcggc
     5281 ggagatgttg aggcaggtct tgcaattgcc gaggtgattt cgggaatgaa aaagccgacc
     5341 gtttccatcg tactcggcgg aggtcattca ataggaattc cgcttgctgt tgcggcaaag
     5401 aaaagcttta tcgcaaagag tgcgtcaatg acggttcatc ccgttagaac aacaggcctc
     5461 acactcggag ttccgcagac ctttgaatat tttcagcgta tgcaggacag aatcacaacc
     5521 tttgttgccg aaaattccaa tattacaaaa gaccgttata atcagcttgt gcttaacacg
     5581 ggtgagcttg taatggatat cggaactatt cttgagggag aagaggctgt tgaagaaggc
     5641 ttgattgacg aggtcggaac agtttccgac gcaattgacg ctctttatga tttgattaaa
     5701 gaaaacaaag agagcaagcc aaagtctgca aaatcacgct caaaaaagca ggaaaaataa
     5761 tttcgggcgt tttgcccgaa tacatatata ttgtgacgct tatgatttgt atgaaatctt
     5821 aattatgcca acccatacgg cttttcagcg tacaaataac agagaagatt tgaaaactta
     5881 ttttttggag ggaatacata accatgtcaa ttttagatgc tattattcag ggtgttgttc
     5941 agggacttac cgaatttttg cccgtatcaa gcagcggcca cctcgcaatc acacagcaca
     6001 ttctcggtgc aggcacaggc gaaagcaatc ttttctttaa tgtaatgctc catgtcggca
     6061 cacttgttgc cgttgttgca ttctaccaca aacttgtgtg ggatcttatc aaagaatttt
     6121 tccttatgct caaggatatt ttcacaggca agttcaaatg gagcaaaatg aattataacc
     6181 gtaatctcgt tgtaatgctc atcatcggtc ttataccgct gttcttgctt tttctgccaa
     6241 tccccggaac agatatgaaa ctcaaggacc ttgcagaccg gctttccgca agtcctgttc
     6301 tcatagtaac tgcctgttcg ctcattctca caagcgtact tttaacaatc ggtattatcc
     6361 gtaaccgcaa gagtgttgcc tcaactcaca aacatcttgc aacaaagagc aatgcaaagg
     6421 caacaagaga tagttttaat gttgttgacg caatccttgt cggtgtaaca caggtttgtg
     6481 ccgcactcct tccgggactt tcccgttcag gctcaacact ttcggtcggt gagatgagag
     6541 gaatctcaaa gcagaaggca cttgactaca cattcgtact cggtatcccg tcaatccttg
     6601 cggcggcact tcttgagggt gtggatgcaa tcaagtcacc cgaaggttta agcaatattg
     6661 aatttcttcc gctgattatc ggaatggcag tttccgcagt tgtaggctat cttgcaatcg
     6721 taattttcaa gtggttcctg aaatcagaca aaatggaaat tttcgtaatc tacacagcga
     6781 ttgtcggtgt tgcatttatt gtaatttcaa tcatcgaaat gaatacgggc gtaaaccttt
     6841 tcacaggtgc gccaattgca taaattttaa atggagtgat tttattggca gctaacaaaa
     6901 ataaagctga caaaactaaa aatactgcgt cgggaagaaa aaagacttct tcaaaaacct
     6961 catcaaaagg tgcaaaaact gcaagcagaa ccaaagcatc cgctgttccg aaaaggagca
     7021 gcggactttc gccgcgtgta agggcaatcc tctatgccgc cgttgcggct gtttttgtct
     7081 gcataatttt cataaaaggt tcaaatgtct ggacaatgat acgcagtttc tttttcggag
     7141 ttctcggatt cggaattttt cttgttcccg taacgctttt ttatttgtgt gtcatgaccg
     7201 aaaaggagaa acaggttgca cgctataagc tgaagctttt cctttgtgtc gtcataattc
     7261 tgtttatcgg tgcgttgatt tacgcaggca gcggtgcaaa atattctgat atgaactttt
     7321 ttgcgtgtct tggcaatctg ttcaaggatg cctccgacac ctcaaggtat atgactttcg
     7381 attgcggaat aatcggcgga ctgctcggct atccgcttgt tgtcctgttc ggcagtacag
     7441 ccgcactttt tacaagcttt gttgtaacag ccgcacttat ccttattgtt gcgaacattt
     7501 ctattaaaga tatgacagcg gcggcgtcaa ggacagccgt acatatccgt gaagcaggtg
     7561 aggaaagggc aagagttcgc agagaacgca gagaagaaaa ccgtgagaga aagcttgccg
     7621 aaagaggcga tatcgacatt gcgcttgaag gcgaaaacag aaagtcctcg ggaagtttta
     7681 ttgacattcc gcttgatgac aaaaagacaa aggtcaagaa agcaaagtcg gcagtaaacg
     7741 gaattgacac ggatattgcc gattcaaagg aagaaaatgc cgcagacaag gatctgtcac
     7801 aggatctcat caacataatc aatcgtgcaa gcaagcctat gggcaaaagt gccgacaaca
     7861 cggtttctgc aattgcagag gatatctcaa acgaaaagtc agcttctaag gctgaacaga
     7921 cagaggtcaa gcctgatgcc gaaactgaga acatcgtcaa aaaagcactt ctcaaaagca
     7981 aaaaagacaa cagtgaggag gccggtgatg aaattgcgga acagcttgac agagctgttc
     8041 gttccgataa ttctcaaaaa gaagaaaaaa agtatcagtt cccaccgatt cagttgctca
     8101 aaccaaagct aaattccgat gacagcgatg caatggaaga aatgcagaat aatgccaaaa
     8161 agcttattga taccctcaca agctttggtg taaaggcaag cattgtaaac atctgccgag
     8221 gtccgtctgt aacacgctat gagttacagc ctgcccccgg tgttaaaatc agcaaaatta
     8281 caaatctctc cgatgatatc gcactcagcc ttgccgcaaa cggtgttcgt attgaagcgc
     8341 ctattccggg taaggcggca gtcggaattg aagttccgaa caaggttgta agcatggtaa
     8401 caatgcgtga attgattgat tcggacaagt tcagaaacag caagagtaag ctcacaaccg
     8461 ttctcggcag agatatctcc ggtgaaattg ttgttaccga ccttgcaaaa atgccgcact
     8521 tgctcattgc aggtacaacc ggttcaggta aatccgtatg tgtaaactca attcttatga
     8581 gtattctcta caaggcaact ccggatgaag taaagctttt gcttattgac ccgaaaatgg
     8641 ttgaattttc aaaatacaag ggtattccgc atttgctcat tcccgtagta tccgatgcca
     8701 aaaaggcggc gggtgcgctt gcatgggcag taaacgaaat gctccagaga tataaaattt
     8761 tctccgaata cgattgtaag gatattgatt catataattc tcttatcgaa aaaaatatga
     8821 attatatgga gaaaaatcca cctgttgtta atgaagaggg agaagaggtt cagcctgttc
     8881 ttgaagtaaa cggacttccc gttgccaaag aaaaaatgag ccgtgttgta attgcaattg
     8941 acgagcttgc cgatttgatg atggcggctc cgagcgaggt tgaggactca atctgccgac
     9001 ttgctcagat ggctcgtgcc gcaggtatgc accttatcct tgcaacccaa agacctaccg
     9061 ttaatgtaat cacaggtctt atcaaggcga atgtaccgag ccgtatttca ctcaaggtaa
     9121 gttcaaacac agactcccgt acaattcttg atacaggcgg cggtgaaaag ctcatcggcc
     9181 gaggcgatat gctgttttcg cctgtaggcg ctccaaagcc tattcgtgtt cagggctgct
     9241 atgcttccga tgaagaaatc gagggtgtaa cccactacat aaagaaagca tattcggctc
     9301 agtataattc tgaaatcgaa gaaaaaatta aaagaattgc tgcagaagaa attgctcagg
     9361 gcaaaaaatc gggtgattca gattcatcat ctgacgaagg tcttgatatt gacagcaaaa
     9421 tggaagaggc aatcaagtgc gtaattgaag caggacaggc atcaacctca ctcttgcaga
     9481 gaagactcaa ggtcggctac gcaagagcag gcagaatgat tgacgatatg gaacaaatgg
     9541 gagttgtagg ccctcatcag ggttcaaaac cgagagatgt tctcatgacc tacaacgaat
     9601 ggctcgaacg cagaaatgca cttgaaaaca gagccgatta ataaataaag gagggttcaa
     9661 tatggctttt ttaccgatga atgcaaagga aatgcgtgat cgaggttgga gcgaagtcga
     9721 ttttgtatat gtttgcggtg acagctatgt tgaccatccg tcattcggtg cggcaataat
     9781 cacgagagtt cttgaggatt gcggttacaa aacagcattc cttgcacaac ctaattggaa
     9841 aaacgatgat gattttacac agttcggaaa accaaggctc ggttttatgg tgtcggcagg
     9901 caacattgac agtatggttg cccactacac ggttgcaaag cgtaaaagac atgatgatgc
     9961 ctatacggca ggcggtaaaa acggcaggcg tcccgacaga gcagtcacag tttactgcaa
    10021 cataatcaga aagctatatc ccgattctcc cattataatc ggaggtcttg aggcatcact
    10081 cagaagattt gctcattatg attattggaa aaacgaggta atgccctcaa ttattttcga
    10141 cagcaaggca gatttgcttg tgtacggaat gggcgagctg cagacaattg aaattgcaaa
    10201 aagactttcc gagggctatc ctgttgaatc tctttacgac atcagaggtg tttgctataa
    10261 aattcgtaca gacgattatg tgccaaagtc ggttgtcgaa cttccaagct acgagcgtgt
    10321 aaaagaggac aagcgtgact atgcaattgc atcacgcagg gagcttgagg aggctgatgc
    10381 agtaaggggc aaaaccctca ttcaaaggca cggaaagtat attcttgtgc agaatcctcc
    10441 gatgcagccg cttgacacaa aacagcttga ttatgtctat tctctgccgt atgagcgttg
    10501 gtatccaaag tgttatgaaa gtctcggcgg cgtacccggt atcaacgaag tactgttttc
    10561 aatcacacac aaccgtggtt gtttcggtgc ttgtaatttc tgctcacttg cttttcatca
    10621 gggcagagcg gtaaccgtca gaagtgaaaa gagtattatt gaggaggctg aaagttttct
    10681 tgataatcca cgattcaagg gctacataag cgatgtcgga ggacctaccg caaacttcag
    10741 acttccgtca tgcgaaaagc agaaaaagct cggactttgt aaaaacagaa gatgtcttgc
    10801 accgacacct tgcccgaata tgcaggtgtc acacacggaa tatcttgata ttctgcgaaa
    10861 gctgagaaat ctcaagggaa ttaaaaaggt gtttatccgc agcggaatcc gttttgatta
    10921 tcttattgaa gatgaaaatg acgagttttt ccgtgaactt gtaaagtatc acatcagcgg
    10981 tcagctgaga gttgcccctg agcattgttc ggcggcggtt cttgacaaaa tgggcaagcc
    11041 gcatattgaa agctacaagc gtttttgtaa gatgttctac gattttacgg gcaaggaaaa
    11101 taaagaccag tatgtagtgc cgtatctgat gagctcacac cccggttcaa cactttcgga
    11161 tgccgtagaa cttgcacttt tctgcaagcg tgaaaacatt cacccaaaac aggttcagga
    11221 cttctatccg actccgggta caatttcaac gagtatgttc tatacggagc ttgatccgta
    11281 cactctcaaa gaggtttatg ttcccaagtc tgaaaatgaa aaggcaatgc aaagggcgtt
    11341 gctgcagtat ttcattcccg aaaacaaacc gcttgtaatc aaagcactca tcaaagccgg
    11401 cagacgggat ttaatcggta acgataaaaa atgtcttgta actccgatgg caggacaaac
    11461 acagggcggt tattcaaaac agaacaacaa ccgcaattcg cagaatcaga agggaagagg
    11521 caaaaacggc aaagataagt tcgcaaagta cagacgaaaa aacaaaaaga aataaaatct
    11581 ttttcatact gaaaatatcg gtatgcgtac ttttcattgc ctgtgtactg cttgtttcat
    11641 tcggaaaacc tctcggaatt cccgattgga acgatgtcta tacatttttc ggaatttcag
    11701 ccgatatgga gtccgatttt tctgtttcgt ttatcaatgt cggttcggct gattgtacgc
    11761 tgattaaatg cggagataaa agtgtgctga ttgacggagg aacaaatctt gtgaccgata
    11821 aaattactgc ctatctcaaa cgcagttcgg taactcatct tgatgccgta atcgtgtcac
    11881 accccgacag cgaccacatc ggttcgctgc ccgatatcat agatgaattc ggtacggatg
    11941 ttgtttattt cggaaaatat tccgacagcc acaaaacacc cgaatacgaa aagcttgtta
    12001 attctataaa agaaaataat ataaaaaccg taattccggt ttcagacaaa cccgttgaaa
    12061 tcggaaatat gacctttaaa ttttatcaac ccgaaaagaa cttcggcaac acaaacgata
    12121 attcgcttgt aacaatgctt gaatatgacg aaaagcgatt tctttttaca ggcgacattt
    12181 cgtcaaaaag ggaagaatca atggttaatt ccgacaaaga attaaaatgc gatgttctca
    12241 aggttgcaca ccacggcagt aagtattctt caagtgaaga ttttcttgca aaggcaagcc
    12301 ccgaaaccgc tgtaataagc gtttcggctg acgatacggc acttcccgat tactatataa
    12361 cagccttgct taacagcgtg tgcaaaaatg tttatcggac agacagcgat aaaaccgtta
    12421 tgataacagt cgaaaacggt gagatttgca ccaaaactca tgcctgaaag gttgactata
    12481 aattaaataa tgttataata ttattgttta actgacaaaa atcgaaagga atttttaaat
    12541 atgggagtat atgaagaact cgtagccaga ggacttatcg ctcaggttac ggacgaagaa
    12601 gaaatcagag agcttgtaaa cagcggtaaa gccacattct atatcggctt tgacccgaca
    12661 gccgacagcc tccatgtagg tcactttatg gctctttgcc ttatgaaaag acttcagatg
    12721 gcaggcaaca agccaattgc actcgtaggc ggcggcacag gtatgatcgg tgatccgtca
    12781 ggcagaacag atatgcgtca gatgatgaca aaggaaacaa ttcagcacaa tgttgattgc
    12841 ttcaaaaagc agatgagccg ttttatcgac ttttctgagg gcaaggctct tcttgtaaat
    12901 aacgcagact ggcttcttga ccttaactat gttgaacttc tccgtgatgt aggcgcttgt
    12961 ttcagcgtta ataatatgct tcgtgcagag tgctacaagc agagaatgga aaagggactc
    13021 agcttccttg aatttaacta tatgattatg cagtcatacg acttctatgc tctctatcag
    13081 aagtacggct gtaatatgca gttcggcggt gacgaccagt ggagcaatat gctcggcggt
    13141 acagagctta tcagaagaaa gctcggcaag aacgcatatg caatgacaat caaccttctc
    13201 ctcaactcag agggtaagaa aatgggtaaa acccagtcgg gtgcagtatg gctttcagcc
    13261 gaaaagacaa gcccctacga tttctaccag tattggagaa atgttgatga cggcgatgta
    13321 atcaagtgcc ttaaaatgct cacattcctt ccgcttgaag aaattgatga gcttgcaaag
    13381 cttgagggca gtgaaatcaa caaagcaaaa gaaattcttg ctcatgagct tacagagctt
    13441 gttcacggca aagaggaggc tgacaaggct caggaggccg caagagcaat cttcagcaac
    13501 aagacaaata ctgacaatat gccttcaaca actcttacag aggctgattt tgcagacggc
    13561 gaaatcggaa ttcttgacct catgaagaag tgcggactca tcccttcaaa cggtgagggc
    13621 agaagactta ttcagcaggg cggtgtttct gttgacgatg aaaaggtaac agatgtttac
    13681 gcaaaggttg caaagtctga ttttgaaaaa ggctatgttg taatcaaaaa gggtaaaaag
    13741 gtttaccata aggcaattct tggctgattt tgaaaattaa ataaagggga ttagggcaaa
    13801 atgaaaaagt ttttttcgga gtttaaggaa ttcataatgc gtggaaatgt gcttgatctc
    13861 gcagtcggcg ttatcatcgg tgcggctttt caggcaatcg taacttctct cacaagcgac
    13921 atcatttcgc cgattctcgg tattttcggc ggtgtcaact ttgaccagtt tcaggttaca
    13981 atcaacggcg caacaattat gtacggtaag tttatcacag cggtaatcaa cttcctgatt
    14041 atggctttcg taatcttcct tatcgtaaag cttgtcaaca aggttatgtc actcggcaaa
    14101 aaaactgaac ccgaagaagc tcccacaaca aaaaagtgtc cgttctgttt cagtgatatt
    14161 gatatcaaag ccacaaaatg tccccattgc acaggcgata tcatcgacct tacagaaaaa
    14221 acagatgaca aaaaataata tcgctgttac ataaacagat attacacaac ttaacaacca
    14281 acaaaaaagc cacagtagaa agttaaaatc tactgtggct ttttaaattg aaaattatga
    14341 ttgagcgtaa cattgttacc gtctgcacaa ttttatatta aaataacaaa aacaaaaata
    14401 caattccgca gaggattgct attgctgcac cgactatgac atcacgcaca taatgcactc
    14461 ctgcacacac tctggacacg gaaatcataa ccgcaatgaa gaggtatgca attcctgccc
    14521 acacattgaa ccgcaaaatt gccattgcaa tgatgaatgc gcttgcggtg tggcggctcg
    14581 gcatactgtc gggcttgctg ttctttgcaa aaacaggcgc aactccgtac cgcacatacg
    14641 ggcgttcttc ttttatgata attcgcagaa gtgttattcc caaaaacacc cccgcaggca
    14701 cagtcagcga ctttaaaaac aaatcttttt gccacaaaaa tgtatataca agcaaaatcg
    14761 gatagcttgc aaatactgtc agcggaaaaa ttttatatac gaatttcaac acattttttg
    14821 cggatttgtt attcttgaag aaatcggtca gtttaatata ttgtttacgg ctaattttct
    14881 acatctccca atgcttttta cactccgatt ttaacataac cgttacacta tttcaacatt
    14941 atatttatca aaccagtagt caagttcgat tatatacgca agaatctgcg gtgctttcat
    15001 cagctgaccg taccacggat ttgtgatttt atcgggattt tcaatgatat cttcaatccc
    15061 ttttcggtca agcagttcgt ttagaattga acgcttttta agaatttttc taaccctttt
    15121 tacacactca ttgaagtaaa tcggattgtg cgttttcggg tacgggcttt tctttcgcca
    15181 gcaaatttct tcgggcagaa tatcctcaaa agcctttcgc acaattccct tttcacggtt
    15241 gccccatgcc tttaattccc acggcatatt gtacgcatat tcaacaagtc tgtaatcgca
    15301 gaacggcact cttacctcaa gtccgctgaa cattgagcat ctgtcttttc gctcaagcag
    15361 acattgcata aaccagtaaa aattcaggta gaacatctct cgcattcgcc tgtcaaggtc
    15421 ggtgtcgctt ttaagctttg gggcaaggct tattgtgtca agataccttt gtctgacata
    15481 ctcctcgccg tttttcagca caccgtcctt gagaatgctc ctgcgaatat cctgactttt
    15541 tgaccacgga aaacaatcct caaaaagtat ttcatggttg tgataccacg gatatccgcc
    15601 gaaaagttca tcggcacatt ctcccgaaag tgcaacggtg tagttttgct tgatttcttt
    15661 gcagaagagc aaaagtgacg agtcaacatc aacatacccc ggcaaatccc ttgcctgaac
    15721 actttcataa agtgcgtcgc acacaagcgt gttgtcaagg acaatttccc tgtgctttgt
    15781 gtcggcattt tttgacatca tcgaaataaa ttcgtagtcg ggagtaggct gaaaattgct
    15841 cttttgaaaa tatttttcgt tgtcacggta ttcaaccgag taggtgttga ttttgccgag
    15901 tttgtgttcc ttgttgtaca ttgatgcggt cttgacaatt atactgctgt caagccctcc
    15961 cgaaaggaaa aagcacagcg gaacatcact cacaagctgt cgctgaattg catcagtcag
    16021 cagatatctc gtcttttcaa tggtagttac ttcattgtct gtatgctctt ttgcctcaac
    16081 ggtgtagtat ttttttcggc gaagctttga gtgcctatac attgcacatt cgcccggatt
    16141 aagttcaaat actcccttga aaactccgaa cgaaggcgtt cttgcaggtc ctaaaaagaa
    16201 aatttcattg agtccctgtt cgtcaatttg aggctttaca accttgcttg caagcaacgc
    16261 ttttatttct gacgcaaaca gcaagccacc gttgtattca tagtaaaaca gaggcttaac
    16321 gccgattctg tccctgcaca aaaacagcga tttgtcgctt gtgttataga ttgcaaaggc
    16381 gaaaattccg ttcagctttt tgcagcagtc aacaccgtat ttaatgtacg atttcagcac
    16441 aacctccgtg tcgctgtgac ctctgaaatg aaaacccgat gctttcagct cctcacgaag
    16501 ctcggctgtg ttgtagagtt cgccgttata gacaatgaca taggtcgtgt ccttgtgacg
    16561 aaccgacatc ggttgtttgc cgttttcacg gtcaattaca acaagccgcc tgtgcataag
    16621 cgccgcccct cggttaatgt acaaaccgtt ttcgtcagga cctcgcctgt caagcgtttc
    16681 agacattctg ttgagcaaat caattctttc gctcatatcc tgtgagtggt caatccatcc
    16741 tgctattccg cacataaagt aacctccgtc aattttgttt gtaatttttg agtgaatata
    16801 aaaagcataa agcggtaaaa agcaaagccg ctcccgaaag ggcagttcct ccgaaaaaac
    16861 tgaaattttc aaccgttgat gttgaaaaaa catcggttgt gacggagaaa agttttattc
    16921 ctaaatgcgt aagcaatgcg gtgataaatc cttgcaaaat tctgcaaaag aaaaataaca
    16981 gtttattcgc cttaatatct ccaagtgccg agaaaatctg aaaatgtacg caaagtccgc
    17041 cgaatcctgc cgaaaatgca acaacctcaa tcggcaaatc tttcgatacc gcagtaaccg
    17101 cattgcaaac ttccgtgagt atcaaaaacg gtttttgaat attttcatcc gtaatgtgtg
    17161 agcagaaaat ctcagtaaat gccgaaaaca gcaccacaaa tgcacagatt gcaaacattc
    17221 cccttgcacc gtcaagaaca gactttacaa gtgcgtcgcc tttttgcgga gtgcttatta
    17281 atgtttccaa attggaatta tcatcaagtt tgtttctgca aacgaatttt aaagcaaatc
    17341 caagtataaa aaccgagatg cattgggcgg caaatataat aagcccgact tcaatgctcg
    17401 aataaagctg tgcaccgata aaattcacaa gaaacccagg gcccgaacac accaaaaaca
    17461 gacctgcccg tttacactca ctttcacttg ccgcaccttt tttataaagc gaatttaccg
    17521 tctttgcacc aacgggataa cctccgacaa gcgagagaat tattgccgat gcaaagcaac
    17581 cgtcaagtcc gaaaagcggt tttgtcagcc atgaaagatg tctgccgatt ttttctgcaa
    17641 gaccgctatc tgtaatatat gaggcaagca ccatgaaagg gaaaagtgac ggtacaagcg
    17701 ttgaaaggca caagcctatg ccgttttcag caccctttga acattctttt gaaaacaaaa
    17761 gagccgtaat gcataaaaga aaaattccga ttgctttaaa aaaattcacc gcctttacgg
    17821 cacttttact caggaacata ttaatcacca attaatatat atgacggcag ttgcataaaa
    17881 ataaatgaga aacgaaaaca ggggagattt ttttgccaaa gaagaaacga attgccgata
    17941 aaaacgaaag attgccttct ccgatgttcg atatgggaac aatgccgtat atagagatgt
    18001 tcggaaaccg cagaataacc gttgagggtt caacgggaat tttgctctat gacagcgaaa
    18061 gcataaaaat taatacctgc aaaaccgtaa tttcattttg cgggagagga atgacattga
    18121 agtgcatttc aagctcctgc gttgaaattg aaggctttgt aaccgacatc aactttcttt
    18181 cgtgaggtga aactatgctc cttaaaataa tacgctttct cagaggaagt gtggacttta
    18241 ccgcaagcgg aaaatttccc gaaagattta tgaacataac cgcaaaaaac ggtgtgaatc
    18301 tctggaatcc cgttccgaaa aagaacgcaa tatcagcctc aatgtacctt tccgactaca
    18361 aaaaaatcag aattcttgca cgcaaatcaa gggttaaaac aagaataaca gccaaacacg
    18421 gactgccgtt tattattaac cgctataaga agagaacagg actttttgca ggtgcggtat
    18481 gcggaattat tctctgtatt tttctttcaa attttatctg gtcggtaaaa atttcgggta
    18541 cagaggaaat cagcaataca tatcttgaat cgttacttgg tgataacggg atttctgtcg
    18601 gtgtgtggaa aaacaatctc gatacagatc agatagaacg caatattcag ctgaaatgtg
    18661 acgatatccg ctggatgagc ataaacataa cgggcagtct tgtcacggtt gaagttaagg
    18721 aaacctacaa aaagccgaag cttgacacct caaaaaatcc ttgcaatgta aaagccatta
    18781 aggacggagt cataacacgg attcaggcat ataacggcag acctgttgtt acaaagggca
    18841 gcggagttgt gaacggtcag attcttgtca gcgggcttga cgaaaccaag cagggcgata
    18901 tgcgttactt gagagcaaac gcaaaggttt atgccgacat taccgaacag caaacgcttt
    18961 caattccgaa aaaagtaacc tacagttacg ctgacgaaaa ttatgttgac agaaaaaatt
    19021 tcagattttt atggcttgat tttccctgta accttgcctt tgaaaactac gacaattttg
    19081 taagcacagt tcacggagaa aatctgttta taaatgatgt tgttctgccg gtaggactta
    19141 aaatcagaac cgaccgtgaa cttgtaacaa ccgaaaactc tgtaagtaaa aacaaggctg
    19201 aggagattgc aaaggctgat tgtattctct atgagatttt tacaaagaat aaaagcaaag
    19261 ttatttcaaa acaaatttcc gttaaagaaa ataaaggtag ctacagggtg acaacagatt
    19321 atatttttaa cgaaaatatt gcagatcagg ttgattttga tgtgacagaa tagaaaagtt
    19381 gtgttataat caatttgtat gttttgcttt gaacgactga aattttgcaa tcggacacaa
    19441 acggagggat aactatgaga attttatgta taggtgatgt tgtaggcagt atcggttgtg
    19501 aatttcttcg caacaatctt cccacgctca aaaaggtgaa aggcgttgat tttgtaatct
    19561 gcaacggtga aaattccgct gacggcaacg gactgactcc cgtgtctgca aagcatcttt
    19621 ttgacagcgg agttgacgca attacactcg gcaaccattc tttccgcaga aaagaagtct
    19681 atgactgcct cgactcaaat cccttcattg tcagacccgc taattttccc gaaaattcaa
    19741 cacccggcaa aggtataatt aatgttgaca cagggcgaag aattgtgtcg attataaata
    19801 ttatgggtaa ttcctatatg gataacaacc tgaattgcgc ttttgaatgt gtagataaaa
    19861 tgattaaaca ggctgaaagc aaaatcataa ttgttgattt tcacgcagag gcaacgggtg
    19921 aaaaaagggc aatgggttat taccttgacg gcagagtttc cgcaatgttc ggaacacaca
    19981 cccatgttca gacctcggac gcacaggttc ttccgaaggg aaccggctac ataaccgaca
    20041 cgggaatgac gggtacaatc cattccgttc tcggtgtaaa gagcgaaatt ataattgcaa
    20101 agctgaaaaa taagcttccg cagcgttttg accttgcaag cggagaatgc aaaatggaat
    20161 gtacgctttt tgatgttgat gacaaaagcg gaaagtgcgt ttcagccgag tcaatgcgta
    20221 ttgaataaca caaatcagca taaagtaaat tgtttccaat tgtcaaaaaa tatgaaacaa
    20281 tcccgattaa gtcggctttt tttgcagata attagtgtat tatactattg caaaaaacaa
    20341 gctgataatg tataatgtta taatgtgaat tattgtaaaa ctgcgtttgt cggtgtaaaa
    20401 tcggcttatg cagttgatta agaaatgtac attaaaagga tgtgcctaaa tgataaacgg
    20461 acaaattctc aaacaggctg ttatttcggg tgcgaataat atttcaaacc aaaaaaacag
    20521 tataaatgat cttaatattt tccctgtacc cgacggagac acaggtacaa atatgtcaat
    20581 gacagtcaat gcggctgttg cagcgcttga agattttgac ggtacctgtg caggtgatgc
    20641 cgcaggtatc acagcctctg caatgctccg tggagcaaga ggcaattcgg gtgttatcac
    20701 ttcactaatc ttccgtggct ttgcccaggg acttaaagac attgaagaag ttgacggcaa
    20761 aaatcttgcc gcagcactcg gaatcggtgt tgacgccgct tacaaggctg ttatgaaacc
    20821 gacagaggga acaattctca cggttgcaag aatggcttat gaggcaggta tcgaagccgc
    20881 taagtatgac agagatgttg tgtctgtatg gcagacaatc gttgacaagg ctaacgaggc
    20941 gcttgcaatc actcctcagc ttttgcccgt gcttaaaaag gcaggcgttg ttgacgcagg
    21001 cggtaagggc ctttgcgtaa tctttgaggg tatgctctct gtaattaaga acggcattat
    21061 gattgaagct gacgatgaca cagctcctgc aaccgtcagc acatttgaaa gtgccgccgc
    21121 tgaatttgac gatgacatca gattcacata ttgcacagag ttcatcgttg gcagaaatgc
    21181 cgagattgaa accgaaatcg gcgagcttcg cagttatctt gaatcaatcg gtgattgcgt
    21241 agttgttgta aatgatgatg agattatcaa ggttcatgtt catacggaac agccgggcaa
    21301 tgctctttca aaaggacttg agttcggtca gcttctcacg gttaaggttg agaatatgaa
    21361 ggaacagcac aagaatgtca agtcaacaaa gaaaaaggct gaaaaggaaa agtttgttcc
    21421 cgcagaacct gaaaacgatt tcggctttgt tgcagttgcc gcaggtaacg gtcttaaaga
    21481 cctctttaaa gacctcggtt gtgacaatgt tgtttcgggc ggtcagagta tgaacccgag
    21541 tacagacgat atctatgagg caattatggc aactcctgcc aagaatgttc tcgttcttcc
    21601 aaacaataaa aacatcattc ttgccgcaga gcagacaatc ccgatggtaa aagacagaaa
    21661 tgtcatcatt gttccgacaa gaacaattcc gcagggtatg acggcaatgc tcaactttga
    21721 tcccgagatt tctgccgaaa gcaacgcaca gcttatgacg gacgcacttg cgtctgtggg
    21781 cacaggtctt gtaacctttg ccgcaagaag cagtgaattt ggcggtaaaa aaatcaaaga
    21841 gggcgacatt atcgcccttg aaaacggcag gcttacaatc acagaaaaga gcacagttaa
    21901 ggctcttgtc aagcttgcaa aggatatggt cagccgtgac acttcattca ttactcttat
    21961 ctacggtgag gatgtaagtg aggaagaggc agaaaaggca tacggagagc ttcgtgaaaa
    22021 gttcggttca agaaccgata ttactcttgt aaagggcgac cagcctgttt actactttat
    22081 tctcagcgta gaataagcat ttaaatcgga ttttttcagg gagcataatt tttacggtta
    22141 tgctcccgtt ttgtataacg gaggtgcggt atgacatcac tttataaaac tcccgttaca
    22201 aatttgaacg gaataggtga aaagcgtgcc gcactttttg caagactcgg gattaaaagc
    22261 gttggagatt tgattaattt ctatccacgc acatacgagg attggagcaa catcaagcat
    22321 attgacgagc ttgaatacgg tgaaaccgtc tgcataaagg ctgttgtcgc aacatctgta
    22381 aacgataccc gaataagcgg cggaagaatt atttccaaaa ccgcagtcta tgatgaaacg
    22441 ggttcaattc agcttgtgtt ttttaacaat aaatacatca gctcaatgct cagacagggc
    22501 ggagaatatt tcttttacgg aaaaatctca tccgacagct acggaatgca gattgtatct
    22561 ccgacttttg ctcctgctgt caagggaaca caaatccgtc cgatttacag acaaacggca
    22621 ggacttccga gtaaatcggt tgaatcagcc gtacgacagg cactttcaat gctgccgtca
    22681 aaaattaagg atcctttgcc cgatgccata agagaaagat ttgacctttg ctcactcaga
    22741 caggcgttgt ttgacattca ctttccgaag aaccgccaac agcttgaagc agcccgaaaa
    22801 aggcttgtta ccgaagagct tgttgtgctg aacctcggaa tgagaaatct gcgtgaccac
    22861 agcagaggag taacctctct tgaaatcaca aaggattatt caaaagaatt tgaatcgctt
    22921 ttgccgttca caatgacaaa tgcccaaaaa cgggcggtgt cggactgcat aaacgatata
    22981 atcaacaaat catctccgct gaaccgactt gttcagggcg atgtaggttc gggcaaaacg
    23041 gctgtggcag cctcggtctg ctacacggtt gccaaaaacg gatttcagac cgcattcatg
    23101 gcgccgactg aaatccttgc ccgacaacat tacaaaacat ttcaaaagct ttttgaaaat
    23161 acggatatca aggttggtct gctgacaggc acactcaggg aatccgaaaa aaaacaaatc
    23221 agaaaatcac ttgcagacgg ttcgattgat atggtaatcg gaacacacgc tctcattacc
    23281 gataaaaccg agtttaaaaa tcttgcgctt gccgtaaccg acgaacagca cgtcgcgttc
    23341 cttgccgatc atcttgggcg tggtnnnnnn nnnnaacagc acagattcgg cgttgcacaa
    23401 agagcaaagc ttctcggcaa gggcaacaat ccgcatctgc ttgttatgag tgccactccg
    23461 attccgagaa cactcggact tatcattttc ggcgacctcg acatttcaat aattgacgaa
    23521 cttccgccgt caagaaaacc cgtgaaaacc gtgttgcgaa cttctgcaat gcgtggggga
    23581 gtgtatgact ttatccgcag tgaagtaaaa cacggcagac aggcgtatat agtttgtccc
    23641 cttgttgaag aaggcgagct tgacgatgtt gcgtcagccg aagaatatgc cgcagatttg
    23701 atgttgcggg aatttcctga catagcagtc ggcatagttc acggacagat gaagtctgat
    23761 gaaaaagacg aggttatgcg taagtttgtc gagaatgagt attccgttct tgttgccaca
    23821 acggtaatag aagtcggcgt tgatgttccg aatgctacgg ttattgtgat tgaaaatgcc
    23881 gaaagattcg gactttcaca gcttcatcag ctgagaggaa gggttggcag aggcagttcg
    23941 cagtcctatt gtattctcat cagcgacaag ggcagtaaga caacaagaga acgacttgag
    24001 gtcatgtgtt caacgaacaa cggttttgtc attgccgatg aagatttgaa aatgagagga
    24061 cccggcgact ttttcggtca cagacagcac ggacttccgc agatgtcaat tgccgatttt
    24121 gccgacacaa aaagccttga actttctcaa aaaattgcag actgcataat ggaccgttac
    24181 ggcgatatcc gaaacgatga aatacgattg ttaaaagcag aggtagaccg cctgtttgag
    24241 cgtggcggac aaaatgcttt aaactgaaaa actataaatg ggagaattaa gtaaatgaga
    24301 aaaaccataa ccaaaacact ttcacttctc tgtatgcttt gcatcattat ctgttccgct
    24361 ttcacaacgg caggtgctgc gtcctatcct aatgatgtaa aaaccgagtc ggactcaatc
    24421 cttcttgtaa atatggattc aggtcagacc gtgtatgaaa aagacgccga ctcaaagcgt
    24481 taccctgcgt caacaacaaa aattatgacc tacataattg cggttgaaaa cattgccgat
    24541 cttgataata caaaaattcc gattaagcag tcggttcttg atgttctcaa aaacacaggc
    24601 agttcccttg cgaatgttga aaatcatgtc ggcaagagta tgactgcaat cgatctgctt
    24661 tacagcatga tggttccctc gggtaatgat gccgcaatgg ttcttgccga ttatatcggt
    24721 gaaggcaatg ttgataactt tgtcaagctg atgaatgaaa aggcaaagga gcttggttgc
    24781 gaaaacactc attttgcaaa tcctgacgga cttcacgatc ccgaccacta cacaaccgcc
    24841 cgtgatatgt acaaaatcac aacctatgcg ctcactcttc ctcgctttga agaaatcaca
    24901 aatacctgta catacacttg tgacggtgac gatacagccc ttgtaacaac caactacctc
    24961 attgacgcca accgtggcgg tgagtattac tatatgtacg caaagggcat caaaacgggt
    25021 acgactaacg aggcaggcag atgtcttgta acgacagcgt cggcagacgg ctattcatat
    25081 atggcaattc tccttcacgc accgtatgag gaaggtgtga ccgaggacta cgcaacaatg
    25141 accgatgcgg cagacctttt ccgctgggca ttgacaagtc ttgaaatgaa cactgttgcc
    25201 acctcgtcaa agccgctttg cgaagtaaag gttaaccttg catggggcaa aaacagtaca
    25261 ttgctttatc ccgaaaccga cctcagtgca attgttccga aagactgcac agatgagaat
    25321 ataattattg aacaggatgt tcccgaaagc gttgacgcac cgcttgacaa aggctcggta
    25381 gtcggcaagg caacaatcta ttacaaagca gatgcaaaca gcgaaaagca aaagcttgcc
    25441 gaggttaatg ttgttgcagg cgaaaagatt gaacgcagcg gaattctcta tgtgttcaac
    25501 atcatcggca cagtatttca gtcctattgg tttatcgtga ttatcgctct tatcattctc
    25561 gttctcatca tttatcttat tatttcaaaa attcacggta agcgtaaaag aaagaagaga
    25621 aatnnnnnnn nnnctgatgc aataaaagtg taattaaatt gtaaattttc tatacttgaa
    25681 atttgaaaac ggtatgttat aattatttta gggaataatt attcggaggt tttcatatga
    25741 agtgtccata ttgcagttat gaagaaagca aggttatcga ctctcgttct gccgatgacg
    25801 gcgaaagaat ccgcagaaga agagagtgcc tgagatgcgg caaacgcttt accacacatg
    25861 aggtgattga aactgttccg ataattgttg ttaagcgtga caaatcccgt gaggtttttg
    25921 accgcaacaa gcttacaggc ggaattctcc gtgcctgtga gaagcgtccc gtttcaattg
    25981 aacagattga aaatatggtc aatgatattg aggctcagct tcagtcaagc cttgaccgtg
    26041 aaatcacttc aaagcaaatc ggtgagcttg caatggatca gctcaagaat attgacgagg
    26101 tttcatatgt ccgttttgcg tctgtatatc gccagttcaa ggatattaac acctttatgg
    26161 acgaactcaa taagcttctt actgaaaaga aatagtttct tataaagaat aattccgata
    26221 ctgtacagcc tttctcggtt gtacagtatt tttttgcgat gtaccgtcgc tctccgctcg
    26281 tgcagatagt ttgcaatatc aaaaacatac tatgcccgag cgtaagtata g
//
LOCUS       DAOMVE010000007        25800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_291_length_25800_cov_2.946786, whole genome shotgun sequence.
ACCESSION   DAOMVE010000007 DAOMVE010000000
VERSION     DAOMVE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 25800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25800
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_291_length_25800_cov_2.946786"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="PLS64_00870"
     CDS             <1..1460
                     /locus_tag="PLS64_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRL42416.1"
                     /translation="RKGYTFKGWDKEIPETMPAENITVKAQWEINQYTITFDTNGGSE
                     IAPITQDYGTEITAPDNPTRKGYTFKGWDKEIPETMPAENITITARWKDTEKPTGEII
                     IGTNKWNEFLNELTFGIFFKDTQEVTINAVDNSGVVFVSYLVTDKELSEAELNSLVFR
                     AYEEPFCIDPNGEYIVYVMLVDENINITYLRSDRITLDNIQPVISGIENGKTYCEAQT
                     VTVDEKYVDTVTVNGTVVTLDADGGFVLPPTNGEQKIVVTDKAGNNAEMTVTVNNGHT
                     FGEWVSDNDGKHTRKCTVDGCDAFETENCSGGNATCTEKAVCDVCGKAYGEFDGTNHE
                     GGVQEWTTRTAFNHEQKWNCCGAVIVASEAHEWKDGVCRECGYVCLHNDADKDHICDY
                     CKKTISEHVDKDKNHICDYCEKTISAHEDAPTEEIKKADTVTAKLPDDSKSPQTGDNS
                     NLILWIALLIISGGVMKGVTAFGKSKKHSAKIKDK"
     gene            1930..3120
                     /locus_tag="PLS64_00875"
     CDS             1930..3120
                     /locus_tag="PLS64_00875"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRL42417.1"
                     /translation="MKISVNIVVPLSVETASENALVGGLLVRSCKKYPDFTVLSKKLS
                     SLYGADLTSSLSKHGESQVIKISVSGLDDRYSLDDVSIAKELSELLCSVIFEPNVKNN
                     AFIESELEQERRQLLDVIDSEFNEKRIYAMGQLIKHMCKDEVFGIKRYGTAEKIKAAT
                     AESLCKAWQNLLKTAKFEILYIGDSPADKAKEVFAKAFANIERNVVTSSTDVVKNVSK
                     EKHITEEMELSQSKLVMGFRTQISAGDEEAVAERLMCAVLGGTASSKLFNNVREKQSL
                     CYYCSSSYDSIKGIMYINSGVEGENLEKAEKAILKEIEDMKNGEITDFEIEATKLAVV
                     NSFKSSSDSVSGIENWYTGRIFNGDLETVEEVSAEVNAVTKEQIVNAANKLLLDTVYV
                     LKNK"
     gene            3135..4412
                     /locus_tag="PLS64_00880"
     CDS             3135..4412
                     /locus_tag="PLS64_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRL42418.1"
                     /translation="MNITEIKSEIAGDSYYKVEHPSGLTIFVYPKEGYTSSYAIFGTR
                     YGSINTKFSVNGGDIITVPDGIAHYLEHKLFESEEGDAFVRYAKTGANANAYTSFEKT
                     CYLFSCTDKFDESLEILLDFVQDPYFTAQTVAKEQGIIGQEIKMYDDSPDWRVMFNML
                     EGMYHNHPVKIDIAGTVETIAEITAEKLYEVYNVFYNLNNMILCVAGNVTVDGVLKVA
                     DKMLKPCEKKEIKNYFETEPYEIKEPYVEQTFPVSMPLFNLGFKEKADKPLNEKQLAC
                     TDILLSALASNTSILYRNLMDSNLINSSFSYELFEGPGYCSVIFGGESRAPKQAAEMI
                     KQYISDIKKNGIDKEDFEIARKSVYGDSVSSLNSVSAISNSIIDYAMQGNEIFAYIDA
                     VANAKLEDINARLAEMLDVDNCTLSVVKQPDEV"
     gene            4415..5290
                     /gene="lgt"
                     /locus_tag="PLS64_00885"
     CDS             4415..5290
                     /gene="lgt"
                     /locus_tag="PLS64_00885"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRL42419.1"
                     /translation="MPYHVQFPGFGWEFDINPIAFSIGDYKVYWYGIIIACGLLLAVL
                     YAWKSAPRYKVDGSKLVNCVFAGMITGIIGARLYFCFFKWDYYGQNPIEILYINNGGL
                     AIYGGIIGALLGGLTVAKIQKMEIMPVLDIAMVGFLIGQGIGRWGNFMNQEAYGSPTD
                     LPWRMMSEGTDNIGVHPCFFYESMWCLLGFVLLHFYGKYKQKYSGQIFFMYLVWYGFE
                     RTVVEGLRTDSLYLPFQVFGADIRVSQVLSAVIFITGIVFLIINRKKEDSFYADYRRK
                     KGIGTGKGRGKEADA"
     gene            5223..6074
                     /gene="folD"
                     /locus_tag="PLS64_00890"
     CDS             5223..6074
                     /gene="folD"
                     /locus_tag="PLS64_00890"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRL42420.1"
                     /translation="MQIIDGKKVSAQVKEEVKRQTLELKETHKITPGLAVVIVGDDPA
                     SRVYVNNKKKACEFVGFKSEEYALPAETTQEELLNLVETLNNKKDINGILVQLPLPKH
                     LDDKAVIEAISPLKDVDAFHAVNVGKIMLGEYDFLPCTPAGVMEMLHSYNIEVSGKNC
                     VVIGRSNIVGKPMAMLLLHENGTVTICHSRTKNLAEICSQADILVAAVGRPKFVTADM
                     VKEGAVVIDVGMDRDENGKLCGDVDFENVKDKCSFITPVPGGVGPMTISTLMKNTLKA
                     CKIQNNI"
     gene            6210..6518
                     /gene="rplU"
                     /locus_tag="PLS64_00895"
     CDS             6210..6518
                     /gene="rplU"
                     /locus_tag="PLS64_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRL42421.1"
                     /translation="MYAVIQTGGKQYKVTNDEVIFIEKLDAEANDTVTFDVVAVGTDD
                     GIKVGTPLVEGAKVTGEVVKNGKGKKIRVCTYKPKKGEKRAKGHRQPYTQVKITSIEA
                     "
     gene            6521..6838
                     /locus_tag="PLS64_00900"
     CDS             6521..6838
                     /locus_tag="PLS64_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HRL42422.1"
                     /translation="MTTVTFLKSDDIICGFEISGHSDFAEEGSDIVCAAISSAAYMTA
                     NTVTEILHINAQVTEDDGLMKVRMSPNDALKACDILSGLKLHLTALSEQYKNFIKVKF
                     SEV"
     gene            6843..7127
                     /gene="rpmA"
                     /locus_tag="PLS64_00905"
     CDS             6843..7127
                     /gene="rpmA"
                     /locus_tag="PLS64_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRL42423.1"
                     /translation="MLKINIQLFAHKKGMGSTKNGRDSESKRLGVKRADGQKVLAGNI
                     LVKQRGTHIHPGENVGKGSDDTLFAKIDGVVRFERVGKDRKRASVYPVEQ"
     gene            7262..8533
                     /gene="obgE"
                     /locus_tag="PLS64_00910"
     CDS             7262..8533
                     /gene="obgE"
                     /locus_tag="PLS64_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRL42424.1"
                     /translation="MFVDTAKIKIKAGDGGDGAVSFHREKYVAAGGPDGGDGGRGGNI
                     VFVADSNLSTLADFRYKRKYAAKKGENGRGGRCRGKNAEDLVIRVPVGTVVKEENSGR
                     ILADVSGDEPYIVAKGGKGGWGNPHFATPVRQVPRFAKPGLPGEEFDVVLELKLLADV
                     GLVGFPNVGKSTLVSVVSQAKPEIANYHFTTITPVLGVVSMGEGSSFVMADIPGLIEG
                     AWQGTGLGHQFLRHVERCRMLVHIVDVSGSEGRDPKEDFITINNELAKFNPELAERPM
                     VVAGNKCDMATDEQIADFKKFVEDQGYDFFPIMAAIRYDVDPLLKKIQEMLSKLPPIT
                     HYEAEPAPIVTVDDFDDHSVIIKNVNGIYTVEADWLLQVMKGINFDDYESLNYFQKVL
                     INGGVIDALRKKGCSDGDTVSIYELEFDFMD"
     gene            8556..8957
                     /locus_tag="PLS64_00915"
     CDS             8556..8957
                     /locus_tag="PLS64_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HRL42425.1"
                     /translation="MENTSVHSLADEISPLTLAFVGDGVYDLLVREYLVRKANRSVGE
                     LNKIKVSLVNCKSQAEFAKILMPTLTEKELAVYKRGRNAAPKCTPKNGSVADYHSATG
                     LEALFGYLQINKEFDRINQLFEIIIHTRFGE"
     gene            8962..9855
                     /locus_tag="PLS64_00920"
     CDS             8962..9855
                     /locus_tag="PLS64_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRL42426.1"
                     /translation="MQMNLKPNSCLAKAGTLFFDYLVIFAGAVLYGISVAMFTSPNNV
                     APGGLTGVATMLNYMFSLPIGIMIFVMNIPLFIWGFIENGKGFLTRTLVATALVSVMI
                     DVLTPILPAYTGDTILAAGFGGVLNGLGLGLIFYRGGSTGGTDIIALNLHKHFPFIST
                     GTIILVSDILVLLMVFFVYHSIENVLYALLVIFISIKVIDAVSYGTSRGNGKLMFVIS
                     DNYEEVSANIMKNLKRGVTYLNGEGAYSGNDKKVIMCAVRPQEVYKITSGIKNVDPNA
                     FIIVTTAGAIKGRGFVNIDSN"
     gene            complement(9907..10971)
                     /locus_tag="PLS64_00925"
     CDS             complement(9907..10971)
                     /locus_tag="PLS64_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase family 2/GtrA
                     family protein"
                     /protein_id="HRL42427.1"
                     /translation="MSHAIIIPIYCENRNTINLCNELQNFGAEHIVVVNDGLNPKSEY
                     FNELTSIGCHLVHLKNNNGKGASLKAGIKYAHDNLYNITGYITCDADGQHTANDVMKI
                     SRMLDLRNGSLILGKRDYKKSKMPINIRIGNRLSSAYFKVITGKSCRDTQTGLRGIPA
                     FLYDTVMKTKGSRFDFEMNFLTKCADMRVPFYFVNIIADCSNCSSNFRLIKDTYLIYR
                     TPLRFATASIGCTIIDLVLFTIFAYILPSHMFFNIMLATLMARVVSGGINFLINRKVI
                     FGNTDNGAKQALRFFILFFCIMCASSLIVSALWFLPIPVTLTKAIVDLLLWTVNYKMQ
                     RIWVFKDSNRLKRTPGSKRK"
     gene            11207..12913
                     /locus_tag="PLS64_00930"
     CDS             11207..12913
                     /locus_tag="PLS64_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL42428.1"
                     /translation="MFKKISAGILSALMITAVAVSGTGCSQTQPASIDYKTADKVADG
                     AILQAFCWDFNTIKESMGDIAAAGFSAVQTSPINECLEGEDGGMDLYGNGKWYYHYQP
                     TDFKIGNYQLGTRDEFKAMCDEAHKYGIKVIVDVIANHTTPATDEVSEDLIEAGGGSL
                     ETLYHKEGRTPLNDFGDRLACTTHEMGGLPDINTERPSFQKYFFNYINDCIACGADGF
                     RYDTAKHIGLSDDPKEDDGFKNNFWEKATKEVDNAENLFIYGEVLQGDNDRLADYIKE
                     IGRTTSSTYGSKIRSGIASGNIDTAVVSDYWIGNADPNIVTWVESHDNYINDCTYNNI
                     DSEQVVLGWAIITARKDGTPLFFDRPYNSSIDNSWGMNRIGTQGDDMYKDNRVSAVNF
                     FRTAMKGEDENLVNPNLDSTALMIERGTKGAVIVNTNDALKVDFETNLADGTYVDRVD
                     RKTEYTVKNGKITCDTDIPENSVVVLYNEGYTEYARPASVGVDSKTEFTYSDDTYEVT
                     LTCSNTDNATYSLDGGKAVSYKDGDKVTIKHGDSDVSKLELRAENAEGVKTYERLEFT
                     YM"
     gene            13125..13943
                     /locus_tag="PLS64_00935"
     CDS             13125..13943
                     /locus_tag="PLS64_00935"
                     /inference="COORDINATES: protein motif:HMM:NF018837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HRL42429.1"
                     /translation="MSELKGAKMEGSKVPAEEIKNPNLVAAIEKMQDDNTTENINKMI
                     DEVMKAKFILPAKVTPKTEAKTTNGQTVMQQSTQVQFRLLENGNKEKFFGVFTDTEEL
                     FKWKDTQNAQKVVTDFDSIAQMVMDPQADVKGFVINPFGKSVTFPKQMVHSFKQQKDY
                     MKLKNQTIEPGSPISLGEPKEYPIDLMAALINHFSTEPTVNAAYLRLIEQNGQKSYFI
                     VVDFFGDMESTFDAISKVANPFLDDEIQLSMMPYSMDFAKNAVKGVEPFYRKEN"
     gene            14184..16388
                     /locus_tag="PLS64_00940"
     CDS             14184..16388
                     /locus_tag="PLS64_00940"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HRL42430.1"
                     /translation="MKRTRKITSIILAALMVLSALVVSAGGVSAATSSGSEVYFDNSK
                     YGWKDVYVYAYGTKENAEWPGELMTKEDSGLYKASFASSFKSEKIIFNNGLEKGNGKE
                     QYPEAAGLSLKAGECKMLTAEKQWIDYGKPDDHAYGYTLTANNTSFSTESLDVKLALK
                     NADKGYYSVDGSAKKEFANGDSVKVGEGKIGNSKVTLTLYATGADGVETEQTYTFKKT
                     FTASKTTFSAKSDGHTTEPEGGYYGTNPEMQLGKHKTISVDGDLSDWDSSMIIAQGVA
                     NDDPRVYMPSSMHEQPWDAYALYSAWDDDNLYFLLELANTTYITSPEDNFAASNEARP
                     WRNSIPMYLALSIDPAKQATGKAVGTNKDGSVYTNPFVWGCTNGTAKDGGTGFTTHID
                     TLVAFDSNNSNGGASIFKADTQDTDGTYMFNYDTRIPIGVTSFQAQDNKNGFKIKYAN
                     GTKSTSLFGINAPKGSRVMGDNLDMNSNWVDFFDEGYKNSYGYVYEVAVPLNTLGIDR
                     SYIETQGIGAMQILTYGTSGMDTLPHDPSMLDQANLEYSYDPSTSHEKEDIDNITVPL
                     ARIGALLPDTEVNEAPFEVNFGANLNSGQSAGTPITLLAESYHATGDVTYSFTVNGET
                     VQNSNTDSCVWTPSADGTYSIGVVAVDANGNKAESTKTFVVGSSSSDETLKGDVNRDG
                     SVTVVDATLVQKYIVKLEDFDAETMKIADVNGNGIIEITDATLIQKIVVNLA"
     gene            complement(16472..17227)
                     /gene="hisF"
                     /locus_tag="PLS64_00945"
     CDS             complement(16472..17227)
                     /gene="hisF"
                     /locus_tag="PLS64_00945"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRL42431.1"
                     /translation="MYAKRIIPCLDVKNGRVVKGMSFVNLVDAGDPVESAMQYDKQGA
                     DELVFLDITASSDSRNITIDMVEKVANSIFIPFTVGGGIRSVEDFNVLLRAGADKVSV
                     NSAAVHRPELISEAAYKFGSQCVVAAIDAKRNGDSWEVYVNGGRTPVGMDAIEWAVKC
                     EKLGAGEILLTSMDEDGQKKGYDIALTRAVSEKVNIPVIASGGAGALEHFYDAFTEGK
                     ADAVLAASLFHFGEIPIPELKKYLNEKGIPVRL"
     gene            complement(17230..17853)
                     /gene="hisH"
                     /locus_tag="PLS64_00950"
     CDS             complement(17230..17853)
                     /gene="hisH"
                     /locus_tag="PLS64_00950"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRL42432.1"
                     /translation="MIAVIDYGAGNIRSVYKALKHIGCECVITRDKDEIMNADGAVLP
                     GVGSFGDTMDTMNSYGIKDTVIEYINSEKPFLGICLGLQLLFPESEESPNVKGLSVFD
                     GTITKIPSGEGLKIPHIGWNSLKINEKSRLFKGIEQNAYVYFVHSYFLNASDRSIVSA
                     QTEYGVTIDAAVEKGNVFATQFHPEKSGKTGLKILRNFADIVKGREE"
     gene            18064..18960
                     /locus_tag="PLS64_00955"
     CDS             18064..18960
                     /locus_tag="PLS64_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRL42433.1"
                     /translation="MITHLLTRIDLMMNSLSKGQKRIALFIEEHYDKAAFMTASKLGE
                     TVGVSESTVVRFATELGYDGYPKLQKAMQEMIRNKLTSVQRIEVTSNRIGNDNVLDSV
                     LNQDIDKIRRTIEETSHEDFARAVDEICKAERIYIFGVRSTAAIANFLAYYFEMIFDN
                     VRVITSGSQTETFEQIFRITDKDVMIGISFPRYSSMSVEAMDFARSRGAHAIAITDSM
                     VSPLVQSADSVLIARSDMASIVDSLVAPLSLINALIVATVLKKKDEVSDTFRRLELVW
                     NRDGVYTNRDREIMGNIEDDDK"
     gene            18947..20188
                     /locus_tag="PLS64_00960"
     CDS             18947..20188
                     /locus_tag="PLS64_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRL42434.1"
                     /translation="MMISKKVVVIGAGAAGLMAGATAALYGASVTIIEKNKRVGRKIM
                     ITGKGRCNLANNCDVQTFINNVPVNGRFLYSAINAFTPEDTIEFFESKGLKTKTERGN
                     RVFPASDKSVDVVDTMHDYAVESGCKIVCDTANALILEDGAVIGVKCEENTYYADSVI
                     ICCGGKSYPLTGSTGDGYTLAKQAGHTIVPLKPSLVPFTSGDKQCKDMQGLALKNVAL
                     RIVDKNSKKTVYSDFGEMLFTHFGMSGPMILSASSHIRDIQPDRYIAEIDLKPALDFE
                     HLDRRIQNDFKENSNRDVSNAFSKLLPRKIIVPVLKRWGVPFDKKCNSITKEERHALC
                     EILKCFTVEISGFRPIEEAIITSGGVKTSEINPKTMESKLVSGLYFAGEVIDCDAYTG
                     GFNLQIAWSTGRLAGENSAYI"
     gene            20230..20898
                     /gene="cmk"
                     /locus_tag="PLS64_00965"
     CDS             20230..20898
                     /gene="cmk"
                     /locus_tag="PLS64_00965"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRL42435.1"
                     /translation="MSIAIALDGPAGAGKSSIAKRVAKALDCIYVDTGALYRTIGLSA
                     TRNSVAPEDENAVKNLLSKITVDLTFNEKGEQIVLLDNEDVSGLIRTPEASMMASKIS
                     AVPIVRAYLLDLQRNMAKTHDVIMDGRDIGTVVLPDAQVKIFLTASPEARAERRYKEL
                     VEKGMDVKYDDILNDVITRDYNDTHRKTAPLKPAEGCVTVDTTELDFEQSVEKIISVI
                     KENI"
     gene            20898..21533
                     /locus_tag="PLS64_00970"
     CDS             20898..21533
                     /locus_tag="PLS64_00970"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL42436.1"
                     /translation="MGQSKALYAVGKAVCKPVFKLLYRYKTIIEEPLPLVGGMIIASN
                     HISNSDPVLLGLSQKKRRLFFMAKIELFKNKFFGALIRALGAFPVERGAGDGKAIKTG
                     EDLIRDGEIMTIFIEGGRSKTGELMRPRSGCALVAQQMQVPVVPACITIVGNKKHTFA
                     KRVIHFGKPLTPEKLGLTPDSGRRELKGATTMIMDEIKRMREQDLIEYKKG"
     gene            21514..23484
                     /locus_tag="PLS64_00975"
     CDS             21514..23484
                     /locus_tag="PLS64_00975"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HRL42437.1"
                     /translation="MSIKKADSAGFCFGVNRAINIVNELLEKGIKVATLGPIIHNMEM
                     VHELEERGCTPVKAVDELTDDTTLVIRSHGVEKSVIDSLVKRGINFEDATCPFVKKIH
                     KIVSNTNPENDVVLIAGNKNHPEVCGIIGHCASDCYTFNNEAELDDLLPNILKENNKQ
                     VQIVAQTTFDTQEWKKCVKKIKKLCTNAKIFDTICNATQVRQTEASQIAAESDFMVVI
                     GDRHSSNTGKLFDICKRQCENTVLIETAAELDLNKVSVAESIGVTAGASTPARIIKEV
                     LDTMSEVKSGETNFEPSFEEMLEESLKNFNTNERVMGTVLSIAPNEVQVDVGRKQTGF
                     IPASELSNDPNARPEDVVKVGDVIELLIMKTNDQEGTIMLSKRRVDAQKGWEELKEKA
                     ESQEVLSGKVTEAVKGGVIVLYNGSRIFIPASQATATREESLEDLVGQDVDFRLIEVS
                     QNGRRRRAIGSIRSVLKEQRAAQREEFWKTCEVGKRYKGVVKSLTSYGAFVDLGGVFG
                     MIHISELSWTHIKHPSEVVNVGDTVDVYIKDINEETKKISLGFKNAEDNPWEILKNNY
                     PEGTVVKATIVGLTTFGAFANIIPGIDGLIHISQIANKRIEKPADVLKVGDVVEAKIT
                     AIDFDKKRVSLSMRALLPEDEQAPAKSEEAAE"
     gene            23552..24880
                     /locus_tag="PLS64_00980"
     CDS             23552..24880
                     /locus_tag="PLS64_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HRL42438.1"
                     /translation="MKRLISLAVAVMLITLLGVCRSRAYDEYNLTDLAACSSVKSDSN
                     GKGGFVCAFSGNTVFSARLVPDFSAYSFSVGGRIRSVSQSGNYTYALVFDDAFTNKYS
                     VYSLNLNNGNVSKNTFVDENFMTSSFSVTDNRIYYIKTDSLKSYVACRDFSSDKKSKI
                     TFSDNVNEVFNNNGKSYARLCDGSIYKLSQSSSTYVADTGELKNIYNAGINRVINEDG
                     FIVSLSDNSRDYVSNATAENVSVSDGKIYSAVNYRLNLKTSEKTKSVSIPDRATAVVS
                     YKKQCAVLLDNGTVQVFGSGDFAEKKTSGNDGSNDNHNSSKSDIQPNNSEYLFSDGIV
                     YGIYSGETVVDFKNKTSAENVYKADGTIAKSGKLKTGFTAVIDSKTYVIAVCGDVTGE
                     GNVNSKDVTLLQKYLCDNAELDGAYLKAADFNLDGEADNRDLVLISRQKN"
     gene            complement(24940..25674)
                     /locus_tag="PLS64_00985"
     CDS             complement(24940..25674)
                     /locus_tag="PLS64_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRL42439.1"
                     /translation="MSGHNKWSTIKQKKGKNDAARAKVFTKIGRELIVAIKEGGSADP
                     SVNSKLKDCIAKAKAANVPNDNIERIIKKASSDADSTNYEAVTYEGYGPNGVAVIVEA
                     LTDNRNRTAGEVRHYFDKFGGNMGTQGCVSFMFSKKGVLVIEREDLEKDEDTVMSDAL
                     ECGASDFEADDDVFTIYTETDDFGAVRDELEKLGYTFVSAEIEMVPSTYTKLEDEETA
                     TKMQKMLDMFEDNDDIQNVWHNWEMD"
BASE COUNT         7906 a         5140 c         5699 g         7055 t
ORIGIN      
        1 caagaaaagg ttataccttt aagggttggg ataaggaaat ccccgaaacc atgccggcgg
       61 agaatataac ggttaaagca caatgggaaa tcaatcagta cacaataacc tttgatacca
      121 acggcggaag tgagattgca cccattacac aggattacgg tacggaaatt accgcccctg
      181 acaacccgac aagaaaaggt tataccttta agggttggga taaggaaatc cccgaaacca
      241 tgcctgccga aaacattaca atcacggcac ggtggaaaga taccgaaaag ccgacgggcg
      301 aaattatcat aggcacaaat aaatggaatg aattcttaaa cgagcttacc tttggcatat
      361 tctttaagga tacacaggag gtaactataa atgccgtaga taacagcgga gttgtgtttg
      421 tatcctattt ggtaacagat aaggagcttt ccgaggctga actgaactct cttgtattcc
      481 gtgcatatga agaaccgttt tgcatagacc ccaacggaga atatattgtt tatgtaatgc
      541 ttgttgatga aaatattaac ataacatacc ttcgctccga ccgtataacg cttgataata
      601 ttcagcctgt aatcagcggt attgagaacg gcaagaccta ttgtgaggca caaaccgtta
      661 ctgttgatga gaagtatgtt gataccgtta ccgtaaacgg tacggtggtt acgcttgatg
      721 cagacggagg ttttgttctc ccaccgacaa acggcgagca gaagatagtt gtcaccgaca
      781 aagcgggcaa taatgcagag atgaccgtaa cagtcaacaa cggtcatacc tttggcgaat
      841 gggtatctga caatgacggt aagcataccc gaaaatgtac cgtggacgga tgcgatgctt
      901 ttgaaaccga aaattgctca ggcggcaatg ccacctgtac cgaaaaggcg gtatgtgatg
      961 tctgcggtaa agcctacgga gaatttgacg gaacaaacca cgagggcggt gtacaggagt
     1021 ggacgacaag aaccgctttt aatcacgaac aaaaatggaa ctgctgtggt gctgtgattg
     1081 tggcaagtga agcacatgag tggaaagacg gtgtgtgccg agaatgcgga tatgtatgtc
     1141 tgcataatga cgcagataaa gaccatatct gcgattattg caaaaagaca atctctgagc
     1201 atgtagataa ggataaaaac catatctgcg actactgcga aaagacaatc tctgctcatg
     1261 aagatgcgcc aaccgaagaa atcaaaaagg ctgacaccgt aacggctaaa ttgcctgatg
     1321 attcaaagtc ccctcagaca ggcgacaaca gcaaccttat tttgtggatt gcattgctga
     1381 ttatcagcgg cggcgttatg aaaggagtaa cggcattcgg taagagtaaa aaacattctg
     1441 ccaaaattaa agataagtaa tattttacgc tctgtcatgg aaatatagtg aatacggcac
     1501 agtaggtata gaaaccgctg tgccgtattt ctttcagaat gactgctttt gaaaaacagg
     1561 cactttctcg gtcatcatac gataagcaaa gacaaagaat aaatgcgaaa tccggaattt
     1621 aatgccaata cttccaaaaa tcggcagaca agtttgtcaa taagttcgta tagttgcatt
     1681 tgtttttatt tatgatacaa ttaaaagcaa caaacttaac agattcttat gcaggtaaaa
     1741 ttcggaaaat atgtcgatag ccttgcacga aaaagtaaaa ttgtttcaaa ttgctattgc
     1801 acttttgtga ttttttctta tatctctttt ataataaatc aaaatgtgat acaatatgac
     1861 tgataatctg aaaacggagt tgattacggt gtttacttta caaacattaa ggacagccgt
     1921 tttaaaacta tgaaaatcag cgtgaatatt gttgttccgc tttctgtcga aacagcgtcg
     1981 gagaatgcgc ttgtgggcgg attgcttgtt cgttcatgca aaaaatatcc cgattttact
     2041 gttctcagca aaaaactcag ctcactttac ggtgcagacc tcacttcatc gctttcaaaa
     2101 cacggtgaaa gccaggtaat taaaatttcc gtatcaggtc ttgatgacag atattctctt
     2161 gatgatgtca gcattgccaa agaactttcg gagcttttgt gcagcgtaat ttttgagccg
     2221 aatgtcaaga acaacgcttt tattgaaagt gaacttgaac aggaacgcag acagttgctt
     2281 gatgtgattg attcggaatt caacgaaaag cgaatctatg caatgggaca gcttataaag
     2341 catatgtgca aggatgaggt tttcggaatc aagcgttacg gtacagccga aaaaatcaag
     2401 gccgcaactg ccgaatcact ctgcaaggca tggcagaatc ttctcaaaac cgcaaaattt
     2461 gagattctct atataggcga cagccctgcc gataaagcga aggaggtttt tgcgaaagcc
     2521 tttgcaaata tcgaaagaaa tgttgtgact tcatctactg atgttgttaa gaatgtttca
     2581 aaagaaaaac atattacaga ggaaatggag ctctcacagt caaaacttgt catgggattc
     2641 agaacacaga tttccgcagg tgatgaagag gcggttgccg aaagacttat gtgtgctgtt
     2701 ctcggcggta ctgcaagctc aaaactcttt aacaatgttc gtgaaaagca gagcctttgc
     2761 tattactgct cgtcctccta tgattcaatc aagggcataa tgtacatcaa cagcggtgtt
     2821 gagggcgaaa atcttgaaaa agccgaaaaa gctattctca aagaaattga ggacatgaag
     2881 aacggcgaaa ttacagattt tgaaattgag gcaacaaagc ttgctgttgt taattcattc
     2941 aagtcaagca gcgacagcgt aagcggtatt gaaaactggt acacaggaag aatctttaac
     3001 ggcgaccttg aaactgttga agaagtttcc gctgaggtta atgccgttac aaaagagcag
     3061 attgtaaatg ccgcaaacaa gctcttgctc gatacggttt atgttctcaa aaataagtaa
     3121 gggaggcgga cagaatgaat attacagaaa ttaaatcgga aattgcaggc gacagctatt
     3181 ataaagttga acatccatcg ggacttacaa tttttgttta tccaaaagag ggttacactt
     3241 cttcatatgc aattttcggc acacgctacg gcagtatcaa cacaaagttt tccgttaacg
     3301 gcggcgatat aattacagtt cccgacggta tcgcccatta ccttgaacac aagctttttg
     3361 aaagtgaaga aggcgatgcg tttgtaagat acgcaaaaac aggtgcaaat gcaaacgcat
     3421 atacaagctt tgaaaaaacc tgctatcttt tctcatgtac ggacaagttt gacgaaagtc
     3481 ttgaaattct tcttgatttt gttcaggatc cctactttac ggctcagaca gttgcaaagg
     3541 aacagggcat tatcggtcag gaaatcaaaa tgtacgacga ctctcccgat tggcgagtaa
     3601 tgttcaatat gcttgagggt atgtaccaca atcaccctgt aaaaattgat attgcaggta
     3661 cggttgaaac aattgcggaa attacagccg aaaagcttta tgaagtttac aatgtgttct
     3721 acaacctcaa taatatgatt ctctgcgttg cgggtaatgt aacggttgac ggtgtgctta
     3781 aagttgccga caaaatgctc aagccttgtg agaaaaagga aatcaaaaac tattttgaaa
     3841 cagagcctta tgaaatcaaa gaaccttatg ttgagcagac tttccctgtt tcaatgcctt
     3901 tgttcaatct cggcttcaaa gagaaagcag acaagcctct taacgaaaag cagcttgcat
     3961 gcacagatat tcttctttcg gcacttgcat caaacacgag catactctac agaaatctta
     4021 tggattcaaa cctcatcaat tcaagttttt cttatgaact ctttgaaggt ccgggttatt
     4081 gctcggtaat cttcggcggt gaatcaagag cgccgaaaca ggcggctgag atgataaaac
     4141 agtatatctc agatattaag aaaaacggta ttgataaaga ggactttgaa attgcaagaa
     4201 agtctgttta tggtgattcg gtttcatcgc tcaactcagt gagcgcaatt tcaaattcaa
     4261 tcatcgacta tgcaatgcag ggtaacgaaa tttttgccta cattgatgcg gttgcaaatg
     4321 caaagcttga ggatatcaac gcaaggcttg ccgagatgct cgatgttgac aactgtactt
     4381 tgtctgttgt aaaacagcct gatgaggtgt aattatgccg tatcatgtgc agtttcccgg
     4441 atttggctgg gaatttgata ttaacccgat tgcattttca atcggtgatt acaaggtcta
     4501 ttggtacgga ataatcatcg cctgcggact tttgcttgcc gtactttatg cgtggaaaag
     4561 tgctccccgt tacaaggttg acggttcaaa gcttgtcaac tgtgtttttg caggaatgat
     4621 aacgggaatt atcggtgcaa gactgtactt ctgtttcttc aaatgggatt attacggaca
     4681 gaacccgatt gaaattctct atatcaacaa cggcggactt gctatttacg gcggaataat
     4741 cggtgcgttg ctcggcggtc tgactgttgc caaaatacag aaaatggaaa ttatgcctgt
     4801 tcttgacatt gcaatggtcg gtttccttat cggacaggga ataggcagat ggggcaattt
     4861 tatgaatcag gaggcatacg gctctcctac ggatttgccc tggagaatga tgagcgaggg
     4921 aacagacaac ataggagttc acccgtgttt cttctatgaa tcaatgtggt gtctgctcgg
     4981 atttgtactt ctccatttct acggaaaata taagcagaaa tattcgggac agattttctt
     5041 tatgtacctt gtgtggtacg gatttgaaag aacagttgtt gaaggactga gaacagacag
     5101 cctttatctt ccgttccaag ttttcggagc agacataagg gtgtcacagg ttctttcggc
     5161 agtaattttt ataacaggta ttgtattctt aattataaac agaaaaaagg aggattcttt
     5221 ctatgcagat tatcgacgga aaaaaggtat cggcacaggt aaaggaagag gtaaagaggc
     5281 agacgcttga gttaaaggaa actcacaaaa taacccccgg tcttgcggta gtaatcgtag
     5341 gtgacgatcc ggcgtcaaga gtatatgtaa acaacaagaa aaaggcttgt gaatttgtag
     5401 gctttaagtc tgaggagtat gcactcccgg cagaaacaac acaggaagaa cttttaaacc
     5461 ttgttgaaac tctcaacaat aagaaggata tcaacggtat tcttgttcag cttccgttgc
     5521 cgaaacacct tgatgacaag gctgttattg aggcaatcag cccgcttaaa gatgttgacg
     5581 cattccatgc cgtaaatgtc ggcaaaatta tgctcggcga gtatgatttt cttccatgca
     5641 cgcctgcagg cgtaatggag atgctccatt cctacaacat tgaagtaagc ggtaaaaact
     5701 gcgttgtaat cggcagaagt aatatcgtag gcaagcctat ggcaatgctt ttgctccacg
     5761 aaaacggcac ggtaacaatc tgtcacagca gaacaaagaa tcttgctgaa atctgttcac
     5821 aggcagatat actcgtagcc gcagtcggca gaccgaagtt tgttactgcc gatatggtaa
     5881 aagagggtgc ggttgtgatt gatgtcggta tggacaggga tgaaaacggc aaactctgcg
     5941 gtgatgttga ttttgaaaat gtcaaggata aatgctcgtt tatcacaccc gtgccgggcg
     6001 gtgtaggtcc tatgacaatt tcaactctta tgaaaaatac tctcaaggct tgcaaaatac
     6061 agaataacat ttgatatact caaaaaatat cgggaaaatt gttgacagaa agttttcttt
     6121 gtggtatagt atataagccg catattgtgg gttttaagtg attcgttcca cctgcacata
     6181 gcgagcccaa tgtaaaggat ggtactaaaa tgtacgcagt tattcagaca ggcggcaagc
     6241 agtacaaggt aactaacgat gaggttatct tcatcgagaa gcttgacgca gaagcaaacg
     6301 acacagtaac atttgatgtt gtggcagtcg gcacagacga cggcatcaag gttggcacac
     6361 cgctcgttga gggtgctaag gttacaggtg aagttgtaaa gaacggtaaa ggcaaaaaaa
     6421 tcagagtttg cacttacaag cctaagaagg gcgaaaagag agcaaaaggt cacagacagc
     6481 cttacacaca ggttaagatt acttcaatcg aagcctaaaa atgactacgg taacattttt
     6541 aaaatcagat gatataattt gcggttttga aatatcgggg cactccgact ttgccgaaga
     6601 gggcagcgat attgtatgtg cggcaatcag ttctgccgca tatatgactg caaacactgt
     6661 caccgagatt ttgcatatta atgcacaggt taccgaggat gacggtctta tgaaagtaag
     6721 aatgtcaccg aatgatgctt taaaggcttg tgatatactc tcaggcttaa agcttcactt
     6781 aactgctctt tcggagcaat acaaaaattt tattaaagtt aaattttcgg aggtgtaagg
     6841 taatgcttaa aataaacatt cagttattcg ctcataaaaa aggtatgggt tctacaaaga
     6901 acggccgtga cagtgaatca aagcgtcttg gcgttaagcg tgcagacggt cagaaggttc
     6961 tcgcaggcaa catccttgta aagcagagag gcacacatat tcaccctggt gaaaatgtag
     7021 gcaaaggctc tgacgataca ctgttcgcaa agattgacgg tgttgtaaga tttgagcgtg
     7081 tcggcaaaga cagaaagcgt gcaagcgttt atcctgttga gcagtaatct caaaatttat
     7141 gagggtggat tttatatcca cccttatttt ttacatttct gtttatacta ataacaaaca
     7201 ggtctgagat tctttacatt tgtactgata attaaacagc aaaactgtta cggagaaaaa
     7261 tatgtttgta gatacagcga aaattaaaat aaaagcagga gacggcggtg acggtgccgt
     7321 ttcgtttcac cgtgaaaaat atgtagcggc aggcggtccc gacggcggtg acggcggcag
     7381 aggcggtaac attgtttttg ttgccgattc caacctttca acacttgccg atttccgtta
     7441 taaaagaaaa tatgccgcaa aaaagggcga aaacggcaga ggcggcagat gccgtggtaa
     7501 aaatgccgaa gaccttgtta tccgtgttcc tgtcggaact gttgtaaaag aggagaacag
     7561 cggaagaatc cttgccgatg tttcgggtga tgaaccttat atcgttgcaa aaggcggtaa
     7621 aggcggttgg ggcaatccac attttgcaac tcctgtccgt caggttcccc gttttgcaaa
     7681 accgggactt ccgggtgaag agtttgatgt tgttcttgaa ctcaagcttc ttgccgatgt
     7741 aggtcttgta ggtttcccga atgtcggaaa aagtaccctt gtttccgtag tttcacaggc
     7801 aaagcctgag attgcgaact accacttcac aacaatcact cccgttctcg gagttgtgtc
     7861 aatgggtgag ggctcatcgt ttgttatggc tgacattccc ggtcttatcg agggtgcatg
     7921 gcagggtaca ggtcttggac atcagttcct ccgccatgtt gagcgttgcc gtatgctcgt
     7981 tcacattgtc gatgtttcgg gcagtgaggg cagagatccg aaagaggatt tcataacaat
     8041 caacaatgag cttgcaaagt tcaatcccga gcttgcagag cgtccgatgg ttgttgcagg
     8101 caacaagtgc gatatggcaa ccgatgagca aattgcagac ttcaagaagt ttgtcgaaga
     8161 tcaaggttat gatttcttcc cgattatggc ggcaatccgc tatgatgttg atccgctcct
     8221 caaaaagatt caggaaatgc tttcaaaact tcctccaatc actcactacg aggcagagcc
     8281 tgctccgatt gtgaccgttg atgattttga tgaccattct gttataatca agaatgtgaa
     8341 cggaatctat acagttgagg ctgactggct gttgcaggtc atgaagggta tcaattttga
     8401 cgattacgaa agccttaact atttccaaaa agttctcata aacggcggtg taattgacgc
     8461 actccgaaaa aaaggctgtt cggacggcga cacggtttca atttatgagc ttgaatttga
     8521 ctttatggat taatattgcg tgatacggag ttaatatgga aaatacatct gttcattcat
     8581 tagccgatga aatttctccg cttacacttg cctttgtggg tgacggcgtc tatgacctgc
     8641 ttgtgcgtga ataccttgtt cgcaaggcta accgcagtgt cggagaactc aataaaataa
     8701 aggtttcgct tgtaaactgc aaaagtcagg ctgaatttgc aaaaattctt atgccgactc
     8761 taaccgaaaa ggagcttgcc gtttataagc gaggcagaaa tgccgcaccg aaatgtactc
     8821 caaaaaacgg ttctgttgcc gattatcaca gtgcaacggg acttgaagca cttttcggat
     8881 acttgcagat taataaagaa tttgacagaa taaatcagct ttttgaaata attatacata
     8941 ctcgtttcgg ggagtgattt tatgcaaatg aatttaaaac cgaattcctg tcttgcaaag
     9001 gcaggaacac tattttttga ttatcttgta attttcgccg gagcagttct ttacggcata
     9061 tcggtagcaa tgtttacatc acctaacaat gttgccccgg gcggacttac gggtgttgcc
     9121 acaatgctaa actatatgtt ctcgttgcca ataggtatta tgatttttgt gatgaacatt
     9181 ccgcttttca tctggggatt tatcgagaac ggaaagggct ttcttacaag aacccttgtt
     9241 gcaacggcac ttgtttcggt tatgattgat gttcttacac cgattttgcc tgcatacacg
     9301 ggcgatacaa ttcttgccgc aggattcggc ggagtgctta acggtctcgg actcggtctt
     9361 atcttttacc gaggcggttc aacaggcggt acagacatca tcgcactgaa tcttcacaag
     9421 cattttccgt tcatttcaac gggtacgata atccttgtgt ccgatattct tgtgctttta
     9481 atggtattct ttgtttatca cagcattgaa aatgtactct atgctttgct tgtaattttt
     9541 atcagcataa aggtaattga tgctgtaagc tacggtactt caagaggtaa cggcaagctc
     9601 atgtttgtaa tttcggataa ttatgaagaa gtgagtgcca atattatgaa aaacctaaag
     9661 agaggtgtaa catacctcaa cggcgagggt gcatacagcg gtaatgataa gaaagttatt
     9721 atgtgcgcag tccgtcctca agaggtttac aaaatcacat cgggaatcaa aaatgttgat
     9781 cccaatgcct ttattatcgt aacgactgca ggcgcaataa agggcagagg ttttgtaaat
     9841 attgacagca actgaaaatt atacaaaaat aacggtggca ttttcgtgtc accgttttcg
     9901 tttgttttat tttcttttgc ttccgggagt tcttttaagt ctgttgctgt ccttgaaaac
     9961 ccatattctc tgcattttgt aattgaccgt ccacaacaaa agatcgacaa tcgcctttgt
    10021 gagtgtaacg ggaatcggca ggaaccaaag tgccgataca ataagtgagc ttgcacacat
    10081 tatgcagaaa aacagaataa aaaatctgag tgcctgtttt gctccgttat ccgtattgcc
    10141 gaagatgact tttctgttga tgagaaaatt aattccgccc gaaacaactc gtgccataag
    10201 cgttgccagc ataatgttaa agaacatatg tgatggcaga atatacgcaa atatcgtgaa
    10261 taaaacaagg tcgattatcg tacagccgat tgatgccgtc gcaaatctca acggagttct
    10321 gtagataagg taggtatcct taataagacg aaaattgctg ctacagtttg agcaatcggc
    10381 tataatgttc acaaagtaaa acgggactct catgtcggca cattttgtga gaaaattcat
    10441 ttcaaaatca aaacgactgc cctttgtttt catcaccgtg tcatatagaa atgcaggaat
    10501 acctcgcagt cctgtttgag tgtccctgca gcttttgccc gtaataacct tgaaatatgc
    10561 cgaggacagt ctgtttccga ttcgtatatt aatcggcatt ttagactttt tgtaatctct
    10621 tttgccaaga atcagactgc cgtttctaag gtcaagcata cggctgattt tcataacatc
    10681 atttgcagta tgttgtccgt cagcgtcaca ggtaatgtag cccgtgatat tataaaggtt
    10741 atcgtgagcg tatttgatgc ctgctttaag gcttgcacct ttgccgttgt tatttttcag
    10801 gtgaacaaga tgacagccaa tacttgtcaa ttcgttaaaa tattccgatt tgggattaag
    10861 accgtcattg acaacaacaa tatgctcagc tccaaaattc tgtaattcat tgcagaggtt
    10921 gattgtgttt ctgttttcac aataaatagg aataattatc gcgtgactca tgcagctgtt
    10981 cctttcattt ttatattttt agtatacaac aatttttctt ttgtgacaat attcaatttt
    11041 tttctattgt aaacatcgta acgatttttt gctcaaaaac atagtttgtg tcattattgg
    11101 cgttttaata aaaaaatatg gcaaaaatgc caaaaactat ttaaaaatca tatcatttat
    11161 gttatactgt atctcaagta agttaaaaac agaaagatgg tgaattatgt ttaaaaaaat
    11221 atcagcagga attctttcgg ctcttatgat tactgccgtt gcagtgtcgg gaacgggctg
    11281 ctcacaaact cagccggcgt caattgacta caaaacagcc gacaaggtgg ctgacggtgc
    11341 gattcttcaa gcattttgtt gggatttcaa cacaataaag gagtcaatgg gggatattgc
    11401 cgcagcaggt ttttcggctg tgcagacatc tccgattaac gagtgccttg agggcgaaga
    11461 cggcggtatg gatctttacg gcaacggaaa gtggtactat cactatcagc cgactgattt
    11521 caaaatcggt aattatcagc tcggtactcg tgatgaattt aaggcaatgt gcgatgaggc
    11581 ccataagtat ggtataaagg ttattgtcga tgtaattgca aaccacacta ctcccgcaac
    11641 cgatgaggta agcgaagatc ttattgaggc cggcggcgga agtcttgaaa ccctttatca
    11701 caaggagggc agaacgcccc ttaatgattt cggcgacaga cttgcctgca ccacgcatga
    11761 gatgggagga cttcccgaca tcaacacgga aagaccttcg tttcaaaagt atttttttaa
    11821 ttacattaac gattgtattg cctgcggtgc cgacggtttc agatatgaca cggcaaagca
    11881 tatcggactt tccgatgatc caaaagaaga tgacggcttt aaaaataact tctgggaaaa
    11941 agcaacaaaa gaagttgata acgcagaaaa tctttttatc tacggcgaag tgcttcaggg
    12001 cgacaatgac agacttgccg actatataaa agaaatcggc agaaccacat caagcaccta
    12061 cggcagtaaa attcgttcgg gaattgcaag cggcaatatc gacacggcag ttgtgtctga
    12121 ttattggatt ggcaatgcag acccgaatat cgtaacatgg gttgaatccc acgataacta
    12181 catcaacgac tgcacataca acaatatcga cagcgaacag gttgtcctcg gctgggcaat
    12241 aatcacggca agaaaagacg gaacaccgct cttctttgac cgtccgtata acagttcaat
    12301 cgacaattca tggggcatga accgaatcgg cacacagggt gacgatatgt acaaggacaa
    12361 ccgtgtttcc gcagtgaatt tcttcagaac agcaatgaaa ggtgaagacg aaaatctcgt
    12421 taatcctaac cttgactcaa ctgctcttat gattgaaaga ggtacaaagg gagctgtaat
    12481 tgtcaataca aatgatgctc tcaaggttga ttttgaaaca aatcttgctg acggaacata
    12541 tgttgaccgt gttgaccgca aaaccgaata tactgttaaa aacggtaaga ttacctgtga
    12601 tacggatatc cctgaaaatt cagttgtagt tctttataat gagggataca ctgaatatgc
    12661 aagacctgca agcgtgggtg ttgactcaaa aacagagttt acatacagcg atgatacata
    12721 cgaggtaact ctcacctgtt caaacacaga taatgccaca tattcccttg acggcggaaa
    12781 ggctgtttcg tacaaagacg gtgacaaagt aacaatcaag cacggtgaca gcgatgtttc
    12841 aaagcttgag cttcgtgcgg aaaatgccga aggcgtcaaa acttacgaaa gacttgaatt
    12901 cacttatatg taaaagatga attttatgct atgcttgcac cgatgtctgt aatgcaaaac
    12961 cacacatcgg tgcagaaaca aaaataaaaa gacggaactt acgactgcat aatcaaaaga
    13021 gatttaaatt caccgaatat tattttcttt gacggtgaag tatccgacga tttgcagtat
    13081 ttcccgacaa aaatcataca gcagaataac ggaggtaaca aagcatgagc gaacttaaag
    13141 gcgcaaaaat ggaaggaagc aaagtgccgg cagaggaaat caagaatcca aacctcgtag
    13201 ccgctattga aaagatgcag gatgacaaca caaccgaaaa catcaacaag atgattgatg
    13261 aggttatgaa ggcgaaattc attctccctg caaaggtaac accaaagaca gaggctaaga
    13321 caaccaacgg tcagactgtt atgcagcagt caacccaggt tcagttcaga cttcttgaaa
    13381 acggcaacaa agaaaagttt ttcggtgtat ttacggacac agaggaactt ttcaagtgga
    13441 aagacactca aaatgcacag aaggttgtta cagactttga cagtattgcc cagatggtaa
    13501 tggatcctca ggctgatgta aagggctttg taatcaatcc tttcggaaag agcgtaacat
    13561 tcccgaaaca gatggttcat tctttcaaac agcagaagga ctatatgaaa ctcaaaaatc
    13621 agacaatcga accgggttca ccaatcagtc tcggcgagcc taaagaatat ccgattgatc
    13681 ttatggctgc tcttattaat catttcagca cagagccgac agtaaatgcc gcatatcttc
    13741 gcttgattga gcagaacggt caaaagagct acttcattgt ggttgatttc ttcggtgata
    13801 tggaaagcac attcgatgca atcagcaagg ttgcaaatcc gttccttgac gatgaaatcc
    13861 agctttcaat gatgccgtat tcaatggatt ttgcaaaaaa tgcagtaaag ggtgttgaac
    13921 ctttctacag aaaagaaaac tgattattca aatattgttt ttggaaaaca ttaaatccgc
    13981 tgacagttta tgtcagcgga ttttttatgg ttaaaatatg ttgcgatata gtcaaaaata
    14041 agctttattt aattaagtct acaaaactct gcacaataac ttgtacaaag aaacaaaaat
    14101 gtgataatga attgaaaaaa caagtgcaaa atgctataat aaaagcaatc gcattaccac
    14161 gattatgaaa tggaggaatc aaaatgaaaa gaacaagaaa aatcacgagc attattcttg
    14221 ccgcactcat ggtgctttca gcacttgtcg tgtcggcagg tggtgtttcg gcagcaacat
    14281 cgtcaggttc agaggtgtac tttgacaact caaagtacgg atggaaggat gtttatgttt
    14341 acgcatacgg cacaaaggaa aatgccgaat ggccgggaga gcttatgaca aaagaggaca
    14401 gcggtcttta taaggcaagc tttgcgtctt cttttaaatc ggaaaaaatc atcttcaaca
    14461 acggtcttga aaaaggcaat ggcaaggagc agtatcctga ggcagcaggt ctttcactca
    14521 aagccggcga atgtaaaatg ctcacagccg aaaagcagtg gattgattac ggcaagcctg
    14581 atgatcacgc ttacggttac acacttacag caaacaatac gtctttttca acagaatcac
    14641 ttgatgtaaa gcttgcgtta aagaatgccg acaagggcta ttactcagtt gacggttcag
    14701 ctaagaaaga gtttgcaaac ggtgattcgg taaaagtcgg tgagggcaaa atcggcaatt
    14761 caaaagttac gcttacactt tatgcaacag gtgcagacgg ggttgaaacg gagcagacat
    14821 ataccttcaa aaagactttt acagcgtcaa agacaacttt ctccgctaaa tcagacggtc
    14881 acacaacaga gcctgagggc ggctattacg gcacaaaccc tgaaatgcag ctcggcaagc
    14941 ataagacaat ctctgttgac ggtgatttaa gcgactggga cagctcaatg attattgctc
    15001 agggtgttgc aaacgatgat ccccgagttt atatgccttc ttcaatgcat gaacagccgt
    15061 gggatgctta tgctctctac agtgcatggg atgacgataa tctttatttc ctgcttgaac
    15121 tggctaacac aacatacata acttctcccg aggacaactt tgccgcatca aatgaggctc
    15181 gtccttggag aaacagtatt cctatgtatc ttgctctgtc aattgaccct gcaaagcagg
    15241 caacaggcaa ggcagtaggt acaaacaagg acggttctgt gtataccaat ccgttcgtat
    15301 ggggttgcac aaacggcact gcaaaagacg gaggcacagg ctttacaact catatagaca
    15361 cacttgttgc atttgactcc aacaactcaa acggcggtgc ttcaatcttc aaggcggata
    15421 ctcaggacac agacggaaca tatatgttca actatgacac aagaattcca atcggtgtaa
    15481 caagctttca ggctcaggac aataaaaacg gatttaaaat caaatacgct aacggaacaa
    15541 agagtacatc actcttcggt atcaacgctc caaagggttc aagagtaatg ggcgataacc
    15601 tcgatatgaa ctcaaactgg gttgacttct tcgatgaagg ttacaagaac tcatacggct
    15661 atgtttacga agttgcagtt ccgctcaata ctttaggtat tgaccgcagc tacattgaaa
    15721 cacagggtat cggtgcaatg cagattctca catacggcac atcgggtatg gatacacttc
    15781 cgcatgatcc ctcaatgctt gatcaggcaa atcttgagta cagctacgat ccttcaacct
    15841 cacacgagaa agaagatatc gacaatatca cagttccgct tgcaagaatc ggtgcgcttc
    15901 ttcccgacac agaagtaaac gaagctccgt ttgaggttaa cttcggtgca aaccttaatt
    15961 cgggacagag tgcaggcaca cctatcacac ttttggcaga aagctatcac gctacaggtg
    16021 atgtaactta ttcattcaca gttaacggtg aaacagttca gaactcaaac acagattcat
    16081 gtgtatggac tccgtcggct gacggcacat attcaatcgg tgtggttgca gttgatgcaa
    16141 acggcaacaa ggcagagtca acaaagacat tcgttgtagg ttcttcttca tcggatgaaa
    16201 ctctcaaggg cgatgttaac cgtgacggca gtgtaactgt tgttgatgca acacttgttc
    16261 agaaatacat cgtaaagctt gaagattttg acgcagaaac aatgaagatt gccgatgtaa
    16321 acggtaacgg cattattgag attacagatg caacattgat tcagaaaatc gtagttaatc
    16381 ttgcttaaac gataaaattt aatcttaaaa acttttaagg cggttgtgca tattctgcat
    16441 aaccgccttt tgtataaacc ttgtatgtaa attaaagcct tacgggaatt cctttttcgt
    16501 tgagatattt tttaagttcg ggaatcggaa tttcaccaaa gtgaaaaagt gatgccgcaa
    16561 ggactgcgtc agccttgcct tctgtaaaag catcataaaa atgctcaagt gcgcccgcac
    16621 cgccgcttgc aattacggga atgttcacct tttcggaaac tgcccttgta agtgcaatgt
    16681 catagccttt tttctgaccg tcctcgtcca tgcttgtaag gagaatttct cccgcaccga
    16741 gtttttcgca ttttacagcc cattcaattg catccatacc gacaggagtt cttccgccgt
    16801 tcacataaac ttcccagctg tcaccgtttc ttttggcatc tatagcggcg acaacgcact
    16861 gactgccgaa tttatatgcc gcctcgctga tgagttcggg acgatgaaca gcggctgaat
    16921 ttacggacac cttgtctgcg ccggctctga gcagaacatt gaaatcttca acgcttctga
    16981 taccgccgcc gaccgtgaac ggaatgaaaa ttgaatttgc aaccttttca accatgtcaa
    17041 tggtgatgtt tcttgaatcg cttgatgcgg taatgtcgag aaatacaagc tcgtcagcac
    17101 cctgtttgtc atactgcatt gcagattcaa cgggatcgcc tgcgtcaaca agatttacaa
    17161 aactcatacc ttttacaact ctgccgtttt ttacatcaag acatggaatt attctttttg
    17221 cgtacatatt tattcctccc tgccttttac aatgtcagca aaatttctga gaattttaag
    17281 tcccgttttg ccgctttttt cggggtgaaa ctgagttgca aaaacattgc ctttttcaac
    17341 agcggcgtca atcgtaacac cgtattccgt ctgagccgaa acaatgctcc tgtcgcttgc
    17401 gttcaaaaaa tatgagtgaa caaaataaac gtatgcattc tgttcgatgc ctttgaaaag
    17461 tctgctcttt tcattgattt taaggctgtt ccaaccgatg tgcggaatct ttaagccttc
    17521 gcctgacgga atctttgtga ttgtaccgtc aaaaaccgaa agacctttta cattcggact
    17581 ttcttcactt tcgggaaaga ggagctgaag tccaagacaa atgcctaaaa acggtttttc
    17641 actgtttatg tactcaataa ccgtgtcttt aatgccgtat gaattcattg tgtccattgt
    17701 atcgccaaac gaaccgacac ccggcaaaac agcaccgtct gcattcataa tttcgtcttt
    17761 atctcttgta atcacacatt cacagccgat atgctttaac gctttgtaaa cactgcggat
    17821 attgcctgcg ccgtaatcaa taactgctat catttttatc aactccgtca tttgctttct
    17881 gttaaacaat tttaccatac aacatttatt attgcaacat aaaccgacgc atatttcata
    17941 atttcgtaaa atttacaata atttaattta tatcttattg taaattgcaa gttcttgtgt
    18001 taaaatgaat tggttaagac agtgtgtctg tttttactat ttataacggg agatattaaa
    18061 aaaatgatta ctcatttgct cacaagaata gatttaatga tgaattccct ctcaaaggga
    18121 caaaaaagga ttgctctttt tattgaagag cattacgata aggcggcatt tatgaccgca
    18181 tcaaagcttg gtgaaactgt cggagtcagc gagagtacgg ttgttcgttt tgcaaccgag
    18241 ctcggctatg acggctaccc taagcttcaa aaagcaatgc aggagatgat tcgcaacaag
    18301 ctcacatctg ttcagcgtat tgaagttacc tccaaccgta tcggcaacga caatgtgctt
    18361 gacagcgttc ttaatcagga tatcgataaa atccgcagaa caattgaaga aacatctcat
    18421 gaggattttg cccgtgcagt tgatgaaatt tgcaaggcag agcgtattta catattcggt
    18481 gtgcgttcaa cagccgcaat cgcaaatttc cttgcgtact attttgaaat gatttttgac
    18541 aatgtgcgtg ttatcacaag cggcagtcaa accgaaacct ttgaacagat tttcagaatc
    18601 accgacaaag atgttatgat cggtatttca tttccccgtt acagttcaat gtcggttgag
    18661 gcaatggact ttgcccgttc aagaggcgct cacgcaattg caatcacgga cagtatggtt
    18721 tctcctcttg ttcagagtgc cgattctgtg cttattgcaa gaagcgatat ggcgtcaatt
    18781 gtagactcac tcgttgcacc gttaagtctt atcaatgctc ttattgttgc aaccgtgctt
    18841 aaaaagaaag atgaggtttc cgacacattc cgcagacttg aacttgtgtg gaaccgtgac
    18901 ggagtttaca ccaaccgtga cagagaaata atgggtaata tagaagatga tgataagtaa
    18961 aaaagttgtg gtaatcggag caggtgcggc aggtcttatg gcgggtgcaa ctgcggcgct
    19021 ctatggtgca tcagttacga taatcgaaaa gaacaagcgt gtcggcagga aaattatgat
    19081 aacgggcaaa ggaaggtgta accttgccaa caactgcgat gtgcagactt ttatcaataa
    19141 tgttcccgta aacggaagat ttctctattc tgcaatcaat gcatttactc ccgaggatac
    19201 aatcgaattt tttgaatcca aaggacttaa aacaaagacc gaaaggggaa atcgtgtgtt
    19261 ccctgcatcc gacaaatcgg ttgatgttgt tgatacaatg catgattatg ccgttgaatc
    19321 gggttgtaaa atcgtgtgtg atactgcaaa cgctcttatt cttgaggatg gtgccgttat
    19381 aggcgttaaa tgcgaagaaa acacctacta tgccgacagc gtaattatct gttgcggcgg
    19441 taaaagctat ccgcttacgg gctcaacagg tgacggctat acacttgcaa aacaggcagg
    19501 acacacaatc gttccgctca aaccgtcact cgtgccgttt acctccggtg ataaacaatg
    19561 caaggatatg cagggacttg ccctcaaaaa tgttgcgctc agaatagttg ataagaacag
    19621 taaaaagact gtttacagcg acttcggcga aatgcttttt acacatttcg gaatgagcgg
    19681 tccgatgatt ttatcggcaa gctcgcacat ccgtgatatt cagcccgacc gttatattgc
    19741 ggaaattgac ctcaagccgg cacttgattt tgaacacctt gacaggcgta ttcagaacga
    19801 tttcaaagag aattcaaacc gtgatgtttc aaacgcattt tcaaagcttt tgccgagaaa
    19861 aatcatagtt cccgttctca aacgctgggg agttcccttt gacaaaaagt gtaattcaat
    19921 tacaaaagag gagcgacacg ctctttgtga aattctcaaa tgctttacgg ttgaaatcag
    19981 cggtttcaga ccgattgagg aggcaatcat aacctcaggc ggtgtaaaaa cttccgaaat
    20041 caacccgaaa accatggaaa gcaagcttgt aagcggcctg tattttgcag gtgaagtaat
    20101 cgactgcgac gcctacacag gcggttttaa tctccagatt gcatggagta ccggacggct
    20161 tgccggagaa aacagtgcat atatctgatt tgattataca taatatattt gattgaaagg
    20221 atgtaattta tgtcaatagc aatcgctttg gacggtcctg caggagcagg taaatcctca
    20281 attgcaaaaa gagttgcaaa ggcacttgac tgtatttatg ttgatacagg agcgctttat
    20341 cgcacaatcg gtctgtcggc aacaagaaat tccgtagctc ccgaagatga aaatgcggta
    20401 aagaatttgc tttcaaaaat cacagttgac ctaacattca atgaaaaggg cgagcagatt
    20461 gttctgcttg acaacgaaga tgtgtccgga ctcatcagaa ctccggaggc atcaatgatg
    20521 gcgtcaaaaa tttctgccgt accgattgta agagcatatc ttcttgattt gcagagaaat
    20581 atggcaaaga cgcatgatgt aattatggac ggcagagata tcggcactgt tgttctgcct
    20641 gatgcacagg ttaaaatttt tcttacagcg tcccccgagg caagagccga aagaagatat
    20701 aaggagcttg ttgaaaaggg tatggatgta aaatacgacg acattttaaa cgatgtaata
    20761 acccgtgact acaacgatac tcaccgcaaa acagctccgc tcaagcctgc cgagggttgc
    20821 gttaccgttg acacaacaga gcttgatttt gagcagtcgg ttgaaaaaat aatttccgtt
    20881 ataaaggaga atatctgatg ggtcagtcaa aggcacttta tgcagtcggc aaagctgtct
    20941 gcaagcctgt tttcaaactt ttgtacagat ataaaactat aattgaagag ccgcttccgc
    21001 ttgttggagg catgattatt gcaagtaacc atatatcaaa ttcagatccg gttcttctcg
    21061 gtttgtcgca gaaaaagcgc agactgttct ttatggcaaa aattgaactt tttaaaaata
    21121 aattcttcgg tgcgttaatc cgtgcgctcg gtgcattccc cgttgaaagg ggtgcaggtg
    21181 acggcaaggc tatcaagaca ggcgaagacc ttatccgtga cggcgagatt atgacaatct
    21241 ttattgaggg cggtcgttcc aaaacaggcg agcttatgcg tccccgcagc ggatgtgctc
    21301 ttgttgcaca gcagatgcag gtgcctgttg tgcctgcctg catcacaatt gtaggcaaca
    21361 aaaagcatac atttgcaaag agagttatcc acttcggcaa acctcttaca cccgaaaagc
    21421 tcggacttac tccggacagc ggaagacggg aacttaaagg tgcaacaacc atgatcatgg
    21481 acgaaatcaa aagaatgagg gagcaggatt taattgagta taaaaaaggc tgattcggca
    21541 ggattctgct tcggcgtcaa ccgtgcaatc aatatcgtaa atgaactgct tgaaaaagga
    21601 ataaaggttg caaccctcgg acctattatc cacaatatgg aaatggttca cgagcttgaa
    21661 gaaagaggat gcactcccgt taaagcagtt gacgagctta ctgacgatac aacgcttgta
    21721 atccgttcac acggagttga aaaaagcgtt attgattctc ttgtaaaaag aggaattaac
    21781 tttgaggacg caacgtgtcc ttttgttaaa aaaattcaca agattgtatc aaacacaaac
    21841 cctgaaaatg atgttgtttt gattgcagga aataaaaatc atcccgaggt ttgcggtata
    21901 ataggtcatt gtgcaagtga ttgttacaca tttaataacg aggcagagct tgatgattta
    21961 ttgccaaata ttctaaaaga gaataataag caggtgcaaa ttgtcgcaca gacaacattt
    22021 gacacacaag aatggaaaaa atgtgtaaaa aagataaaaa agctatgtac aaatgcaaaa
    22081 atatttgata caatatgtaa tgctacgcaa gttaggcaaa ccgaagcttc tcagattgcc
    22141 gccgaatccg actttatggt tgtaatcggt gacaggcaca gctccaatac gggaaagctt
    22201 tttgatatat gcaaaagaca gtgtgagaac acggttctca tcgaaaccgc tgccgagctt
    22261 gatttaaaca aggtttcggt tgccgaatca atcggcgtaa ctgcgggtgc ttcaacgcct
    22321 gcaaggataa taaaggaggt actggataca atgagtgaag taaaatccgg tgaaacaaat
    22381 tttgaaccat cttttgagga gatgcttgag gagtccctca aaaactttaa tacaaacgag
    22441 agggtaatgg gcacagttct gagcatagct cccaacgaag ttcaggtaga tgtcggcaga
    22501 aagcagacag gctttattcc tgcttcagag ctttcaaacg atcctaacgc aagacccgaa
    22561 gatgtagtta aagtaggcga tgttattgag cttcttatta tgaagaccaa tgatcaggaa
    22621 ggcactatca tgctttcaaa gcgcagagta gatgcacaga agggttggga agagctcaaa
    22681 gaaaaggcag agtcacagga ggttctctcc ggcaaggtta ctgaggcagt taagggcggc
    22741 gtaatcgttc tttacaacgg ctcaagaatc tttatccctg catcacaggc aacagcaact
    22801 cgtgaagaga gccttgaaga cctcgtaggt caggatgttg atttcagact tatcgaagtt
    22861 tcacagaacg gcagacgcag aagagctatc ggttcaatcc gcagcgtact caaggagcag
    22921 agagctgctc agcgtgaaga attctggaaa acctgcgaag ttggcaagcg ttacaagggc
    22981 gttgttaagt cacttacaag ctacggcgca tttgttgacc tcggcggcgt gttcggcatg
    23041 attcacattt cagagctttc atggacacac attaagcatc cgagcgaggt tgttaatgta
    23101 ggcgatacag ttgatgttta cataaaggat atcaatgagg aaacaaagaa gatttctctc
    23161 ggcttcaaga atgctgaaga caatccgtgg gaaatcctca agaacaacta tcccgaggga
    23221 acagttgtta aggctacaat cgttggtctt acaacattcg gtgcattcgc aaacatcatc
    23281 cccggcattg acggtcttat ccatatttct cagattgcaa acaagagaat tgaaaagccc
    23341 gctgatgtac tcaaggttgg cgatgttgtt gaagctaaga ttactgcaat cgacttcgac
    23401 aagaagcgtg taagcctttc aatgcgtgca cttcttcctg aagacgagca ggcacctgct
    23461 aagtctgaag aagctgctga ataatcagaa cttttgtggg catccgaaag gatgcccttt
    23521 tgttttaaat taatttaatc gggtacaggc tatgaaacga ctgatttccc ttgctgtggc
    23581 tgttatgctt atcacacttc tcggtgtgtg cagaagccgt gcgtatgatg aatacaatct
    23641 gactgacctt gccgcttgtt cgtctgtaaa aagtgacagc aacggcaagg gcggatttgt
    23701 gtgcgcattt tcgggaaata cagtgttttc agcaaggctt gtgcccgatt tttctgcata
    23761 cagtttttct gtcggcggtc ggataaggag tgtgtcacag agcggaaact acacttatgc
    23821 acttgttttt gacgatgctt tcactaacaa atattcagtg tattctctta atcttaataa
    23881 cggaaatgtt tcaaagaaca cttttgttga cgaaaatttt atgaccagtt ccttttcggt
    23941 aaccgataat cgcatttact atatcaagac cgacagcctg aaatcttatg ttgcgtgtag
    24001 ggatttttca tctgataaaa agtcgaaaat aactttttca gacaatgtaa acgaggtttt
    24061 taataataac ggaaagtcat acgcaagact ttgtgacggt tcaatttaca agctctcgca
    24121 atcgtcatca acctatgttg ccgatacagg tgaacttaaa aatatttata acgcaggaat
    24181 aaacagagtt ataaacgaag acggatttat tgtttcgctg tcagataatt caagggatta
    24241 tgtttcaaat gccacagccg aaaatgtttc ggtatctgac ggaaaaatat attctgccgt
    24301 gaattatcga cttaatctga aaacatccga aaaaacaaaa agcgtgtcaa ttcccgacag
    24361 agcaactgct gttgtgtctt acaaaaagca atgtgctgtt ttgcttgata acggaacggt
    24421 tcaggtattt ggctcgggtg attttgcaga aaagaaaaca agtggcaatg acggcagtaa
    24481 cgataatcac aattcttcta aatcagatat tcaaccgaat aattccgaat acctgttttc
    24541 tgacgggata gtttacggga tttacagcgg agaaaccgtt gtcgatttta aaaacaaaac
    24601 ttctgccgaa aatgtatata aggcggacgg tacgattgca aagagcggaa agctgaaaac
    24661 aggctttact gccgttatag attcaaagac ttatgtcatt gcagtttgcg gagatgtgac
    24721 gggcgagggc aatgtaaatt caaaggatgt cacgcttttg caaaaatacc tctgtgataa
    24781 tgcagaatta gacggtgcat atctcaaagc cgccgatttt aaccttgacg gcgaagcaga
    24841 taaccgtgac cttgtcttaa tttcaagaca aaaaaattga caaaacaaaa accgccccaa
    24901 tcggagcggt ttaatttatt ttgtcagaaa taaactttat cagtccattt cccagttgtg
    24961 ccatacattc tggatatcat cgttatcctc gaacatatca agcatcttct gcatcttagt
    25021 tgcagtttct tcgtcctcaa gctttgtata tgtgcttgga accatttcaa tttcagcaga
    25081 aacgaatgtg tagccgagct tttcaagctc atctcttaca gcgccgaaat catctgtttc
    25141 tgtgtaaatt gtaaatacat cgtcgtcagc ctcaaagtct gatgcaccgc actcaagagc
    25201 atcgctcatc acggtatcct catccttttc aaggtcctca cgctcaataa cgagaacgcc
    25261 cttctttgag aacataaagc ttacacagcc ctgtgtaccc atattgccgc cgaatttatc
    25321 aaagtaatgt cttacttcgc ctgctgttct gtttctgttg tctgtaagtg cctcgacaat
    25381 tacggcaacg ccgtttggac cgtagccctc atatgtaaca gcctcgtagt ttgttgaatc
    25441 tgcatcgctt gacgctttct tgatgattct ttcaatgttg tcgttaggta catttgcagc
    25501 ctttgctttt gcaatacaat ccttgagttt tgagttaacg gaaggatcgg cacttccgcc
    25561 ttccttgatt gcaacaataa gttctctgcc gatttttgta aatactttag ctcttgcggc
    25621 atcgttctta ccttttttct gcttgattgt actccatttg ttatgtcctg acattaatat
    25681 cattcctttg tcaataaagt ataattatct tattaagtat atcacacaca cctttgtgat
    25741 gtaaacaaaa aaagttatta cagttttgta ataactccat tgtcaaatat cgcctgtggt
//
LOCUS       DAOMVE010000008        25293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_305_length_25293_cov_3.063515, whole genome shotgun sequence.
ACCESSION   DAOMVE010000008 DAOMVE010000000
VERSION     DAOMVE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 25293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25293
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_305_length_25293_cov_3.063515"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLS64_00990"
     CDS             complement(<1..270)
                     /locus_tag="PLS64_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRL42440.1"
                     /translation="MNKTELIAAVAEQSGLSKKDAEKAVSSTIDTIINTVAEGEKIQI
                     AGFGTFEQRQRNARTGCDPRTGKSIEIPASKVPAFKAGKGFKDVVN"
     assembly_gap    364..373
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<374..1095)
                     /gene="mazG"
                     /locus_tag="PLS64_00995"
     CDS             complement(<374..1095)
                     /gene="mazG"
                     /locus_tag="PLS64_00995"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRL42441.1"
                     /translation="MNFTEKENYNFNDLVEIVKILRAPDGCPWDREQTHKSIRSNFIE
                     ETYEAVEAIDTDDLDLLKEELGDVLLQVALHAEIESEQGTFDINDVCDGICKKLIIRH
                     PHVFGDVNADTTEKVLKNWDAIKMKTKSQKTQTQAILSVSKALPSLMRSTKIQQKAAK
                     VGFDWENVNGALDKLFEECEELKAAVENNDVENQREELGDVLFSAVNVARFLNIDSEH
                     ALYDACDKFTDRFSSVEKLANER"
     gene            complement(1174..3030)
                     /locus_tag="PLS64_01000"
     CDS             complement(1174..3030)
                     /locus_tag="PLS64_01000"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HRL42442.1"
                     /translation="MSKLKTKKYNAVANAEKDASQTFVKGAAILTLSMVIVKVFGLLD
                     KVILTGIFSGVSFEQAVAGGTETVNFASFGLGLYSNAYEIFVVIFTVATGGLPIAISR
                     LVSESTAQKRFNDVKQIHRVSIPFFVIVGILSFAILVGASFIYVQIIESPYSLLGMLC
                     LSPTVLFGCLVSIYRGYYEGQRNMFPTAVSEIIEATIKLFIGAFLAYIIAHLGMNSYA
                     ESGTVFGLYIANQTEAYQTIVSFSVAGAIMGITLGSAASFVFYILKFKIQGDGIPEEY
                     YRNSVEARSKRETFNKILKTAIPVAMGSLIMSVGSLIDQVIVQRVLLNMIETNPQALQ
                     AQYSKYFTADMFYADTKTIHTSPWGCYSAALTFLQLVTAVTQVFGSSAMPNVTSAWTK
                     GNKDELKKSIETVIRLTMLFTFPMALGMMALSFPIMGLVYNDPLITDVGGRILLIMGV
                     TTIFGAASTPVCSMLQGIGRVDLPMKLYTVCMAVKIGITWMFVSVPEINVQGATVGSM
                     ITYAIMTIVGMYLLIKYSKIKPDLFGTTVKPLIGAIASAASAFGAYHLMESIIGSARM
                     SIAVSLAVAIFVAVIVYIAVLLILRAFTATELKFLPKGEKIAKTLEKLGLIR"
     gene            complement(3055..3450)
                     /locus_tag="PLS64_01005"
     CDS             complement(3055..3450)
                     /locus_tag="PLS64_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRL42443.1"
                     /translation="MGKFDEIFDDVVVNAKACASAVSQKASTVYDVSKHKITAAEIRG
                     DINKKLRELGALTYKSEVHGTDNSQQIKLIVSEISDLKDNLDVVNEHIAVAKDQRRCP
                     GCNAVIPKNSVFCNICGIKIDDTDEDFEL"
     gene            complement(3597..5036)
                     /gene="hemZ"
                     /locus_tag="PLS64_01010"
     CDS             complement(3597..5036)
                     /gene="hemZ"
                     /locus_tag="PLS64_01010"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HRL42444.1"
                     /translation="MNLYVKNHNFHFELENLTRLFFPNEKITVIRDFSEPQPPYIYTE
                     VSDKITISVNIGSFNKSETAVKKLTDDDNELVSAQLLYKLLCDFTGLTQPWGILTGVR
                     PVKLLRRLAEESSEEQAVKKFEKDFFVSNEKIALSRETEHNERKILELSKPESFSLYV
                     GIPFCPSRCSYCSFVMASIERAEKLIEPYTKLLCEEIKRTAEIANKLGLRLETVYFGG
                     GTPTTLSAEQLDTVLGTVNKSFDMSTCREFTVEAGRPDTIDIAKLFALKENKVDRISI
                     NPQTVNDEVLKTIGRKHTAQQFFDAFELARKCGFDNINTDLIAGLPTDTPESFKNSLD
                     SIVRLNAECITVHTLCMKRASRLTTEGVTLDLQQARDAREMLAYTQNILGQNEYIPYY
                     MYRQSRMVGNLENVGWSKKGFESLYNVYVMDETHTILACGSGGVTKLKRNNPDYLERI
                     FNFKYPYEYIDRFDELIQRKSGIMQFYGQ"
     gene            complement(5038..5664)
                     /locus_tag="PLS64_01015"
     CDS             complement(5038..5664)
                     /locus_tag="PLS64_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL42445.1"
                     /translation="MIDVKIFNVTVLMTNCCLLTDRKTGKMAVTDPGAPNEELNSEIE
                     KNGGKLEYVILTHGHYDHISYAKQLAEKFDAKIVTGEKNNEFLSNPALNLTEKHRLSL
                     KPFSADILLKDGDNFMLGNTEIKYITTPGHTSGCGSYIFDDTIISGDTLFCESYGRTD
                     LPTGDDAEMAKSIRKLKNLEGDYRVIPGHGPTTTLDHERKYNPLMRIL"
     gene            complement(5661..6116)
                     /gene="dtd"
                     /locus_tag="PLS64_01020"
     CDS             complement(5661..6116)
                     /gene="dtd"
                     /locus_tag="PLS64_01020"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRL42446.1"
                     /translation="MKAILQRVTKAKVEVDSKTVGEIGTGFLILLGVENGDTEKEADA
                     LAAKIAGLRIFTDENDKMNLALADVNGSVLVISNFTLCADCSHGRRPNFMAAARPDTA
                     DPLYEYFCKKMHDNGIERVEKGIFGADMKVSLVNDGPVTIEINTKDLKI"
     gene            complement(6132..8330)
                     /locus_tag="PLS64_01025"
     CDS             complement(6132..8330)
                     /locus_tag="PLS64_01025"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRL42447.1"
                     /translation="MGKFSNVAHYQDFTELKKILSASAANTYDMDKIEKAYKFAEKAH
                     GDQRRVSGIPYILHPVSVACIVAELGMDTDSVCGALLHDTVEDTGVTLEEVTKLFGAD
                     VAKLIDGVTKISKIPYSTREQQQAENIRKMLIAMADDIRVIIIKLADRLHNMRTMNCM
                     PEQKRRDKSLENMQVFAPIAHRLGIKTIKDELEDRSLQYLDPIGYKEIEDDLLMTEEG
                     RDKFIESIKNQILAKTEGTIPGVYISGRVKSINSIYRKTYMQGKTMDQIFDIFAVRVI
                     VDTVSDCYNVLGVVHDIFKPIPNRFKDYISMPKPNMYQSLHTTVLDKNAIPFEIQIRT
                     WEMHYTAEYGIAAHWKYKLGMGAGGKNNDKMEENIKKVKDMILDQLEAEDVTDIAKNI
                     RNDFTENDVYVFSPRGDVFNLPQGSTPIDMAYLIHTQVGHRMVGAKINGKIVPIDYKL
                     KTGDICEIITQKEEHPNRGWVDICKTASAKSKIRSWYKHEKRDENIAEGRQMLDKEFK
                     RHGINLSEEEYPDFLQKLMIKKQYNSMDDFYAAVGYGGIQLWKIMPRLKEEYQKAYAS
                     DIEEIDVPQAPVKRPKASAGVVIEGADDVLVKFSKCCNPLPGDDIIGYITRGYGVSIH
                     KRHCTNVPKDISKSEEPERWVSCYWEDEVGEAFRSTLQITATDRTGLLADVTIKLSDM
                     HIFIHSLNSREAGNGTAVVTATIDVMGRNHLRGVISKLSDINGTIEVKRL"
     gene            complement(8330..10372)
                     /gene="recJ"
                     /locus_tag="PLS64_01030"
     CDS             complement(8330..10372)
                     /gene="recJ"
                     /locus_tag="PLS64_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRL42448.1"
                     /translation="MKLWSVAKLDKTKAGEIQSRYELPAIVAMLLQIRNITTKDEIEG
                     FLYNDSFIADPFEIKDMDKAVERINKALDCGEPICVYGDYDADGVTSTALLYSYLETI
                     DANAMYYIPSRETEGYGMNKNAVDKLNERGIKLIITVDNGIAAAQEVAYASSLGIDTV
                     VTDHHMPSGELPKACAVVDLHREDCKSRFKNLSGVGVAFKLIMALEGEYCDTTTLLDN
                     YSDLLSIGTIGDVVELKDENRVFVKHGLESITNTDRPGLQALIKNSGLTGKTVSSTNV
                     SFTIVPRINAVGRLGLSEKSVSLLLTEDYDEAAEISSQLCVDNSERQEIERDILEKID
                     GIIRRKPSLVNDKIIVIDGENWHQGVIGLIAAKVKEAYGKPAIVISKLGDIAKGSGRS
                     VEGFPLCDAVFACSDLLTHRGGHPMAVGLSLDSENIPAFRRKINEFADNFGKMPYDKI
                     NIECKLNPAYINLDLIDSLSLMQPYGAGNPTPVFGLYNMTLKNITPLSANRHLRLTLS
                     RNNIQITAMKFFTSTEEFPYKIGETLDLAVNLDRNEFNGNVSVSVIVKDIKSSDCDTE
                     KLLDSRTNYEDFCRGKQLDRSQLSDLMPDRNDFALLYRYLKSNGGYAFETATLVHMLD
                     NRLSFGKIKVILEAMRELRLIEISEGMKTSKISVLPVNGRVDLESAPIIKKLREVF"
     gene            complement(10409..12076)
                     /gene="recN"
                     /locus_tag="PLS64_01035"
     CDS             complement(10409..12076)
                     /gene="recN"
                     /locus_tag="PLS64_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRL42449.1"
                     /translation="MLRTLYIENIAVIEKTSIDFENGLNVLTGETGAGKSIIIDAINA
                     IMGQRTSKEIIRTEADSAFVSAQFDDINQSIVEKLDELGFSDDEGMLILQRTLNRNGK
                     SSCKINGKPATVSMLKELSKHLINIHGQHESYELFSPETHIDYIDRFGGCEKLLADYK
                     KCYDEYKILKKKLNSADSDESERLKEIDLLSYQVNELTESDVKQGEEQELESERTALM
                     SFEKIFSLLNDAKMALDGDENYGGGLESVDSASTALQKASSYNAEYEEIANTLTDAYY
                     NLKDCCDSISDAIDSLESDPQRLEEVEERLDLINRLTRKYNCPSDELSDLAEKYQARL
                     DELMNYDRNRDELAEKCRESEEKMLAAAEKLGTLRRKTAKTFATKVKSEMAFLNMPNV
                     ELVPYFEKCEPNSKGTDKMELLISANPGETPRPVAKIASGGELSRMMLAIKTVLASTD
                     TVDTLIFDEVDTGISGSAAEKVGLKLREVSKSSQVLCVTHQAQIAALADYHYLIRKQV
                     EKGRTFTNVTLLDHNGRAGELARIIGGVDITDAALKHAESMLEKYNN"
     gene            complement(12091..12537)
                     /gene="argR"
                     /locus_tag="PLS64_01040"
     CDS             complement(12091..12537)
                     /gene="argR"
                     /locus_tag="PLS64_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HRL42450.1"
                     /translation="MKTGRHARILDIIAEHPIETQDELLTRLREEGFKATQATISRDI
                     KDLRLVKTLGSDGKYRYVSASRSSTDIRTNFSNLFSTSVNSIDVAQNLVVIKTLSGMA
                     QAVCAALDSADYPSVVGTIAGDDTIFIACRTADLAVSLTEELKKLI"
     gene            complement(12537..13394)
                     /locus_tag="PLS64_01045"
     CDS             complement(12537..13394)
                     /locus_tag="PLS64_01045"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRL42451.1"
                     /translation="MKFALVVNLSKERAIKYAKKISLFLLDKNAEIAMIEECSQYFKG
                     IHIHYSKNITELFEYCDMAITVGGDGTIIHAAKYAAKADKQLIGVNVGRLGFAADVEP
                     HEYEQLERLITGDYTTEERILLDVEVIKEDGSKHYLAVNDAVVARGQLSKTIDLHLTL
                     DGDEISKYRADGLLFATPTGSTAYSLSAGGPILAPKMECILMTPVCPHSLFSRSVLFS
                     GESELSVHVKIPEECCCVLTIDGEKNVPILATDRVVIRKSDLKLKLALLHNRNFYKLL
                     NEKLKERDF"
     gene            complement(13407..14213)
                     /locus_tag="PLS64_01050"
     CDS             complement(13407..14213)
                     /locus_tag="PLS64_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005362886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRL42452.1"
                     /translation="MKKRLDILVYEKGFTDSREKAKAIIMSGQVYVDNQKADKCGTSY
                     DENVKIEVRGNTQKYVSRGGLKLEKAIDNFDFDLKDKITMDIGASTGGFTDCMLQNGA
                     KKVYSIDVGYGQLAWKLRNDPRVVNLERTNMRKVTREQVPDEIDFFSVDVSFISLKLI
                     LPVARQLMSENAQAVCLIKPQFEAGREKVGKKGVVRDPAVHVEVVRKIFDFCLENGFD
                     VLNLDYSPIKGPEGNIEYLIHLRKSDDPKSYTDVTPEQLVENSHAALDKK"
     gene            complement(14215..16086)
                     /gene="dxs"
                     /locus_tag="PLS64_01055"
     CDS             complement(14215..16086)
                     /gene="dxs"
                     /locus_tag="PLS64_01055"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRL42453.1"
                     /translation="MGEYELLSKIKSPHDVKKLNDEQLEKLCTEIREKLVETVSVNGG
                     HLSPNLGVVELTVALERSFNLPKDSIVWDVGHQAYTHKLLTGRYEQFDTIRKKGGISG
                     FPKRAESKYDDFNTGHSSTSISAAYGIARAKHLMGDQSYTVAVIGDGSFTGGLAFEGM
                     NNSGRFNKNFIVVLNDNKMSISPNVGALSRYLTTVRIQPWYMRVKKITEKILLHIPLI
                     GKPLKRFLQGGKSKLKNLVYKNTLFDCFGFTYLGPIDGHNIDELENAFECAKKVNSPV
                     LVHVVTKKGKGYQPAEDNPGGFHGIGRFDIDSGEPLSSHKGFSAEFGKTMCEFAEDDK
                     KICAITAAMSGGTGLTEFSRLFKERFFDVGIAEEHAVTFGCGLAAKGMRPVFAVYSTF
                     LQRAYDQLLHDAALGKLHLVIGVDRAGIVGDDGETHQGVFDVSILNTIPNTTIFSPAY
                     FDGMRKSLSTSIYICDSLAVVRYPRGGELYRPDDFGEENLSYDVYGNPNCKNLLITYG
                     RLFSYACKAKETLAKQGIEICILKLCRIKPIDENAVDFAADFDNVWFFEEGIKNGGIA
                     RNFSDLLCCTQFCGDYHIKAINDKFVKQMSVSEALHELKLDDDGMVNTITKNLKEKN"
     gene            complement(16126..17001)
                     /locus_tag="PLS64_01060"
     CDS             complement(16126..17001)
                     /locus_tag="PLS64_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRL42454.1"
                     /translation="MTKDYAKNIENALKSCLPSTDCREKKLIDAMAYSLEAGGKRVRP
                     YLVFSFNSLCGGSEESATAFACAVEMIHTYSLIHDDLPCMDNDDLRRGKPSNHKVFGE
                     DIALLAGDALLTLAFETLTNEKTVKICGTSACCRGVSVLSKYAGATGMIGGQVIDLMS
                     ENTNAPIEVLREMDYKKTACLIKAACELGCISAGANDKQIKAASEFGECIGIAFQIQD
                     DILDVTSSDEELGKPVGSDAENSKSTYVSLLGIEKCRELVDELTEKAVNALHGFDGDT
                     KELEEFAYKLAKRNK"
     gene            complement(16994..17218)
                     /gene="xseB"
                     /locus_tag="PLS64_01065"
     CDS             complement(16994..17218)
                     /gene="xseB"
                     /locus_tag="PLS64_01065"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRL42455.1"
                     /translation="MENKNLTFEQANELLEKTVSKMESGELTLEQTMEEYAKACELLA
                     YCMKQLEQCKGKIIDINERISKLSKGDDND"
     gene            complement(17208..18143)
                     /gene="whiA"
                     /locus_tag="PLS64_01070"
     CDS             complement(17208..18143)
                     /gene="whiA"
                     /locus_tag="PLS64_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HRL42456.1"
                     /translation="MSFSSDVKKEICSVEQFDREALKAELYGMLLFSRNFSPNKITFT
                     TESNYAGNRLIFLLQNLYMPIIEKQSALRTKSSDSHLYKIIVVDGNDCKRIFEDFGHT
                     DGQITLRVNRANLSSEELSQSFLRGVFLSCGSVSDPMKSYHLEFCVPHKNLSQDLCKI
                     LSEITECTLIPKTVVRSGSYIVYFKESEQICDLLTYIGSPIRSMEIMGTKAIKQVRNN
                     VNRRINGEVANIGKIATASAKQLSAIEHIKKTVGIDSLPEDLREIAYLRLENPDMSLR
                     DLGQNLSTPISRSGANHRMQRLLEYAGTEVKTNGK"
     gene            complement(18153..19016)
                     /gene="rapZ"
                     /locus_tag="PLS64_01075"
     CDS             complement(18153..19016)
                     /gene="rapZ"
                     /locus_tag="PLS64_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HRL42457.1"
                     /translation="MEFVIITGMSGAGKTGALHVLEDIGYYCVDNLPSSLLSTLYRLC
                     LKSEDDSMKRVAVVVDVRGNEKFEEMCSQIEHFKMHYENVKVLFFDAMTDRLVVRYKE
                     TRRRHPLSDKLKDGSVLSAVELERELLLPIKRTADYNIDTTYMSNKQLRERIMSMFME
                     DTSQSITLTFMSFGFKYGIPLEADLIMDVRCLPNPFYIPELKHLTGLDKDVRDYVLES
                     EETTEFLKRLLSMLDFSVPLYLKEGKSELVVGIGCTGGKHRSVTIAMQLNDHFREKGY
                     RCVIQHRDIDR"
     gene            complement(19037..19942)
                     /gene="murB"
                     /locus_tag="PLS64_01080"
     CDS             complement(19037..19942)
                     /gene="murB"
                     /locus_tag="PLS64_01080"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRL42458.1"
                     /translation="MDTTDIIYNCAQKLDCDARRFEPMSRHTSFKIGGKADVYIKVTN
                     LSQLMKILKECDVCKEKYILLGNGSNVLVPDEGIHGTVLRLDGDFRNISLIDDTTIYC
                     GAGAALGSLCKFAQKCGLSGLEFAWGIPGTVGGALFMNAGAYGGEMKDVVYSVSHITQ
                     NGDIGRTEAENLEFGYRTSVYRKNGCIITGAVFKLKKDDPEEIQNRMNDYMNRRSTKQ
                     PLEYPSAGSVFKRPEGAFAGALIEQCGLKGKTVGGAQVSEKHAGFIINKSKATADDVK
                     QLVSEIQKTVENETGYKLECELIFL"
     gene            complement(20011..20979)
                     /gene="hprK"
                     /locus_tag="PLS64_01085"
     CDS             complement(20011..20979)
                     /gene="hprK"
                     /locus_tag="PLS64_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRL42459.1"
                     /translation="MSVEFSVPLSQIAEALNLTEVYVAENYKETNISTVEINRPGLEL
                     TGYLEFFDNKRIQVLGNTEFSYLGRYGPEAQKMVIDSIFSFGPPAVIICRDIEPSNAI
                     LESAKLHKVSIFSTPQSTSDLTASLVQYLNKELAPRITRHGVLVEVYGEGCLLTGDSG
                     VGKSETAIELIKRGHRLVADDAVEIRRTAQTTLYGQSPENIRHFIELRGIGIINARKL
                     FGMGAIKLQEKIDMVINLEQWDSAKVYDRMGLDNEYMKILGVEVPTLTIPVKPGRNLA
                     VIIEVAAMNNRQKKMGYNAARELLKNLGMAVEDLPPSPKVIVDTWE"
     gene            complement(21154..21744)
                     /locus_tag="PLS64_01090"
     CDS             complement(21154..21744)
                     /locus_tag="PLS64_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42460.1"
                     /translation="MNKKLFLLPIMAIVLILGGCSNSNRIDTSSLVQTITAENKSGKA
                     VYNFYLLENSEDVQSVSVEADSLEKAVISAKKAYIPALTLSKLELYLIDSNLGEKTLK
                     TDIDYISKETAISPLTYTALSDTKTLQLFSKDKNALKKVKEHIVLLKNNNNNLSVTSL
                     SIFNNFKGKNNGYLSICYISSDKELKADIVKTVTEK"
     gene            complement(21737..22804)
                     /locus_tag="PLS64_01095"
     CDS             complement(21737..22804)
                     /locus_tag="PLS64_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HRL42461.1"
                     /translation="MQSNVTFPSKRLMLSLFVCSVLPILLMGRSFLQSDNIFSSLASI
                     GFGIFFTLLCFVPFYSIKHRTRLDFSAFAKNTSQTGFFFVSSYYCMYFTVITVSFLIR
                     FCDMFVSGVNPDVDTKVIAFLILCICVYGASKGINPLSRVSIFVLAFLIIAFAVIFFG
                     NIKSFEFAPNSTFVTPDFSNIRSNVADCLIFAFLPVIFGFNSTSTKNLSIKQPVITTL
                     IIFGTLMLTLFFTDFVIGAYSAQQPFSVQLLSKTAYFGDMKGFDSFYLAAVTACIFVF
                     VTAVLISVSNCIVNTSSKRTLVFVSIIAFIMFVLFVCADSYNAVKELLLNNIFLTVLN
                     FISALLIPIIYLFVYRRKLYE"
     gene            complement(22804..24258)
                     /locus_tag="PLS64_01100"
     CDS             complement(22804..24258)
                     /locus_tag="PLS64_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HRL42462.1"
                     /translation="MYNSLKENISTLKKMFKNSADFTVREMNLKGQCSIKSAIITIEG
                     MCGKDTLALSVINPLLDYYFDCQSPDKVFDLIKNTVLTSSEIVEFTTIDEAISFSTSG
                     FALLVVDGCSRMLAIGAQGFSFRSVSEPESEVVQRGCREGFTEPLRINMTLIRRRIKS
                     PDLVFETITSGYSSNTQMMICYLQNSVSKQILKAIRERLENCNLKMILASGYLSSYLE
                     DNNSKSLFSGVGISERPDTVCGKLSEGRVAILIDGTPSVIIIPHLFAEEFQSVDDYSN
                     RPYYAAFIRILKYISFLIAVFLPGIYTAFAQFHPEYFPTGLLVKTSDSLSQTPLPVTL
                     EVILITFIYEVMREAGLRIPKPLGHAVSIVGALVIGESAVSAGIISSSTLMVVATAAI
                     CSYVTFALYPPIMVLRFFCVIAGGMFGLWGIVLLTCGVLVNMCSKTSVGVPYMSSLAP
                     FSWRSMRDVFVRADWKRLSKHTIKVQKFPETEEM"
     gene            24340..25008
                     /locus_tag="PLS64_01105"
     CDS             24340..25008
                     /locus_tag="PLS64_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRL42463.1"
                     /translation="MLISIIRTILLYAFVILAVRLMGKRQMSDMQPSELVITMIVSDI
                     ASIPMQNTSQPLLSGVIPVAILVALEITASIIMMKWSGFRSLVCGKPVIVIKDGRILQ
                     SAMKKLRLTTEDLSVRLRQQGIFSIEDVQYCIVETNGEVSVLEKPEKRKPSAEDFGLK
                     IKDNKIETVVVSNGKILENSLNLCEKEKGYVKNILQKKNIKQSNVFLMTLDALGNYNI
                     IKKE"
     gene            25013..>25293
                     /locus_tag="PLS64_01110"
     CDS             25013..>25293
                     /locus_tag="PLS64_01110"
                     /inference="COORDINATES: protein motif:HMM:NF025634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HRL42464.1"
                     /translation="MKRIYIALSLVLAALCFASVETGFISGKTDSFIAKIECADRLMS
                     RLEFEDALKICEETEKEWYDNAKLIDTMLIHDYVDNIGLSISRMTVFAQ"
BASE COUNT         7007 a         5528 c         5017 g         7731 t
ORIGIN      
        1 gtttactaca tccttaaagc ccttgcctgc cttgaatgca ggaacctttg aagcaggaat
       61 ttcgattgat ttacctgttc ttgggtcaca acctgttctt gcatttctct gacgctgttc
      121 aaatgtgccg aaacctgcga tctgaatctt ttcgccttca gctactgtat tgatgattgt
      181 gtcaattgtt gaagaaactg ccttttctgc atccttcttt gaaagtccgc tctgctcggc
      241 aactgcagca ataagttctg ttttgttcat aataataaac ctccaattaa taattcttag
      301 ataatttaac ttcatcccaa agggaatcga gtacagataa cggtgccgtt ttcatatcta
      361 ttcnnnnnnn nnncgctcgt ttgcaagctt ttcaacagac gaaaaacggt cggtgaattt
      421 gtcacaggca tcgtagagtg cgtgttcgct gtcaatgttc aaaaatcttg caacattaac
      481 tgctgagaaa agcacatcac cgagttcttc acgctgattt tcaacatcat tgttttcaac
      541 agcggcttta agctcttcac attcttcaaa aagcttatca agcgcaccgt ttacattctc
      601 ccagtcaaag ccgaccttag cggccttctg ctggattttt gtactgcgca tgagcgacgg
      661 aagtgccttt gacacgctga gaattgcctg tgtctgagtt ttctgggatt ttgttttcat
      721 cttaatggca tcccagtttt tgagaacctt ttcggtagtg tcagcgttga catcaccgaa
      781 aacatgggga tgacggataa tgagcttttt gcagattccg tcacaaacat cgtttatatc
      841 aaatgttccc tgttcacttt caatttccgc atgcagagca acctgcagaa gaacatcacc
      901 gagttcttct ttaagcaaat caaggtcgtc ggtatcaatt gcctcaacag cctcataggt
      961 ttcctcaata aaatttgagc ggattgactt gtgagtctgt tctctgtccc acggacaacc
     1021 gtcgggtgca cgaaggattt ttactatctc aacaaggtca ttaaaattat aattttcttt
     1081 ttctgtaaag ttcattcgtg acatctccga taatcttatt agacaatgca aattttatct
     1141 gagaagcgga aacaaccgca acagatatat attttaccta attaatccta atttttcaag
     1201 tgtttttgca attttctcac ctttcggcaa aaatttcagt tctgttgccg taaaagcacg
     1261 caatatcaac aatacagcaa tataaacgat aaccgcaaca aagattgcaa ctgcaagcga
     1321 aactgcgatt gacattcttg cagagccgat aatgctttcc ataaggtgat atgcgccgaa
     1381 tgccgaagcg gcacttgcaa tcgcaccgat aagcggtttt actgttgtac cgaataaatc
     1441 aggcttgatt tttgaatact tgataagcag atacatacct acgattgtca taattgcata
     1501 ggtaatcatt gaaccgacag ttgcaccctg aacattgatt tccggaacgc ttacaaacat
     1561 ccatgtaatg ccgatcttga cagccataca aactgtgtat agcttcatcg ggaggtcaac
     1621 tctgccgatg ccctggagca tactgcatac aggagttgac gccgcaccga atattgttgt
     1681 tacacccatg atgaggagaa ttcttccgcc tacatctgta ataagcggat cgttatacac
     1741 aagtcccata atcgggaacg aaagtgccat cataccgaga gccatcggga atgtaaaaag
     1801 cattgtaagg cggataacag tttcgattga ctttttgagt tcgtccttgt tgccctttgt
     1861 ccatgcactt gttacattcg gcatagcact tgaaccgaac acctgtgtta cggctgttac
     1921 aagctgaaga aaagtgaggg cggctgagta gcagccccaa gggctggtgt gtattgtctt
     1981 tgtgtcggca tagaacatat cggctgtaaa atacttactg tactgtgcct gcagagcctg
     2041 cggattagtt tcaatcatat tgaggagtac acgctgaaca attacctggt caataagcga
     2101 acctacgctc ataatgagtg aacccattgc aacaggaatg gctgttttga gaatcttgtt
     2161 aaaagtttcc cttttggaac gagcctctac ggaattacgg tagtattctt ccggaattcc
     2221 gtcaccctga attttaaatt tgagaatata gaacacaaat gatgcggcgc ttccgagggt
     2281 gatacccata attgcaccgg caaccgagaa tgaaacgatt gtctgataag cctcggtttg
     2341 atttgcaatg taaagtccga aaaccgtgcc tgactcagca taagaattca taccgaggtg
     2401 cgcaatgatg taagcgagga atgcaccgat aaagagcttg attgttgcct ctataatttc
     2461 cgaaacggca gtcgggaaca tgttgcgctg accttcgtag tatcctctgt aaattgaaac
     2521 gaggcaaccg aataataccg tcggagaaag gcagagcata ccgagcaatg aatacggtga
     2581 ctcaataatc tgaacataga taaagcttgc accgacaaga attgcaaatg aaagaatgcc
     2641 gacaataacg aagaaaggaa ttgaaaccct atgaatttgt tttacatcgt taaatcgttt
     2701 ttgtgccgta ctttcagata caagtcttga aattgctatc ggcagaccgc ccgttgcaac
     2761 cgtgaaaatt acgacaaaaa tttcgtaggc attactgtaa agacccagac cgaaagatgc
     2821 aaaattgacg gtttccgttc cgcctgcaac agcctgttca aaagaaacac cggagaaaat
     2881 acctgtaaga ataaccttgt caagaagtcc gaaaaccttg acaattacca tacttaaagt
     2941 aagaattgcc gcacctttga caaaggtttg agaagcgtcc ttctcagcgt ttgcaacggc
     3001 attgtatttt ttagttttta atttagacaa aaataatcac ccattaataa aaaatcaaag
     3061 ttcaaaatcc tcatcagtat cgtcaatctt gataccgcag atgttgcaga atacagaatt
     3121 tttaggaata acagcattgc atccgggaca tcttctctga tcttttgcca cggcaatatg
     3181 ttcgtttaca acatcaagat tatctttcaa atcggaaatt tccgaaacga taagcttaat
     3241 ctgctgtgaa ttgtctgttc cgtgaacctc agatttgtat gtaagagcac cgagttcacg
     3301 aagctttttg ttgatatcgc ctctgatttc agcggcagta atcttgtgct ttgaaacatc
     3361 ataaactgtt gacgctttct gcgaaacagc agatgcacag gcttttgcat tgacaactac
     3421 atcgtcaaaa atttcatcaa attttcccat attaatccga cctttcttaa aattatctca
     3481 tcagaaaaac aaattatgaa caagaattct gttttgttca atatatgaca atttcacttc
     3541 cgtatgcaat cacatatgtc gtattataac accgttgaca gcattaatca actgtgttat
     3601 tgaccataaa actgcattat cccggacttt ctttgaataa gttcatcaaa acggtcaatg
     3661 tattcgtacg gatatttaaa attgaatatt ctttcgagat aatcgggatt gttgcgtttc
     3721 agttttgtaa caccgcccga accgcaggca aggattgtgt gggtttcgtc cataacataa
     3781 acattgtaca ggctttcaaa gccttttttt gaccagccga cattttcaag attgccgacc
     3841 atcctgctct gacgatacat atagtacgga atgtactcgt tttgtccgag aatattctgc
     3901 gtgtatgcaa gcatttctct tgcatctctc gcctgttgca aatcaagcgt tactccctcg
     3961 gttgtaagac gggacgctct tttcatgcac agcgtgtgaa ccgtaataca ttccgcattc
     4021 agcctgacga ttgaatcaag tgaattttta aagctttcgg gggtatctgt cggcaaacct
     4081 gcaatgaggt ctgtgttgat attatcaaag ccgcattttc ttgcaagctc aaaagcgtca
     4141 aaaaactgct gagcagtatg ctttcttccg atagttttca gcacctcatc gtttacagtc
     4201 tgcggattta tgctgattcg gtcaacttta ttttctttta aagcaaaaag ttttgcaatg
     4261 tcaattgtat cggggcgacc tgcctcgaca gtaaattcac gacaggttga catatcaaag
     4321 cttttgttta ctgttccaag caccgtatca agctgttcgg cactcagagt agtgggagtg
     4381 ccgccgccga aatatacggt ttcaagcctt aaaccaagct tgtttgcaat ttcggctgtc
     4441 cgtttaattt cttcgcataa tagctttgtg tacggttcaa tcagcttttc agccctttcg
     4501 attgacgcca ttacaaatga gcaataactg caccttgacg gacaaaaagg gattccgaca
     4561 taaagactga acgattcggg ttttgaaagt tcaagaattt ttcgttcgtt atgctcggtt
     4621 tcccttgaca atgctatttt ttcgttgctg acaaaaaagt ctttctcaaa cttttttaca
     4681 gcctgttctt cacttgattc ttcggcaagt cgtcttaaaa gctttacggg gcgaactccc
     4741 gtaagtattc cccacggctg tgtaagtccc gtaaaatcac aaaggagttt gtacaaaagc
     4801 tgtgccgaaa caagctcgtt gtcatcatcc gtaagctttt tcacagctgt ttcggactta
     4861 ttaaaagagc cgatattcac cgaaattgtg attttatccg aaacctccgt atagatatac
     4921 ggaggctgtg gttcagaaaa atctcttatt accgttattt tttcatttgg aaagaaaagc
     4981 cttgtaagat tttcaagttc aaaatgaaaa ttgtgatttt ttacatataa attcatatta
     5041 aagaatcctc atcagcggat tgtattttct ctcgtggtca agggttgttg tcggtccgtg
     5101 tcccggaatc acacggtaat caccctcaag atttttaagc tttctgattg actttgccat
     5161 ttcggcatcg tcacctgtgg gaaggtctgt tctgccgtat gattcgcaga aaagagtatc
     5221 tcccgaaata attgtgtcat caaaaatata tgaaccgcaa ccgcttgtat gtcccggcgt
     5281 ggtgatatat ttgatttcag tgttgccgag catgaaattg tcaccgtctt taagcagaat
     5341 atcggctgag aacggtttga gtgaaagtct gtgcttttcg gtaagattga gtgcaggatt
     5401 gctgagaaat tcgttattct tctcgcccgt aacaattttt gcgtcaaact tttccgcaag
     5461 ctgttttgca tagcttatgt gatcgtagtg accgtgtgta agaattacat attcaagctt
     5521 tccgccgttt ttctcaattt cagaattgag ttcttcattc ggtgcgcccg gatcggttac
     5581 agccattttg cctgtttttc tgtctgtaag caaacaacag tttgtcataa gaaccgtaac
     5641 attaaaaatt tttacatcta tcatattttc aaatcctttg tgttgatttc aatagtaacg
     5701 ggtccgtcat tcacaagcga aaccttcata tcggcaccga aaattccctt ttcaacacgc
     5761 tcaattccgt tatcgtgcat ttttttgcag aaatattcgt agagcggatc ggctgtgtca
     5821 ggtcttgctg cagccataaa gttagggcgt ctgccgtgtg agcagtcggc gcagagggta
     5881 aagtttgaaa ttacaagcac cgaaccgttt acatcggcaa gagcaagatt cattttatca
     5941 ttttcatccg taaagattct cagacctgca atttttgcgg caagtgcgtc cgcctctttt
     6001 tcggtgtcac cgttttcaac tccgagaaga ataagaaaac ccgtaccgat ttcgcccacg
     6061 gtttttgaat caacctcaac cttggccttg gtaactcttt gtaaaattgc tttcataaat
     6121 tataacctca attacagtct ttttacttcg attgttccgt tgatgtcgga aagctttgaa
     6181 attacaccac ggaggtgatt tctgcccatt acatcaattg ttgcggtaac tacggcagtg
     6241 ccgttcccag cctctcttga attaagcgaa tgtataaaaa tgtgcatatc cgaaagcttg
     6301 attgtaacat ctgcaagaag tcctgttctg tcggttgcgg taatctggag cgtacttctg
     6361 aacgcctctc ctacctcgtc ctcccagtaa cagcttaccc atctttcggg ttcttcactt
     6421 ttactgatat ctttcggcac atttgtgcaa tgtcttttgt gaattgaaac gccgtagccc
     6481 cttgtaatat agccgataat atcgtcaccc ggcagaggat tgcagcactt tgagaatttt
     6541 acaagaacat cgtctgcacc ctcaattaca acgcctgcgg atgccttcgg tcgctttacg
     6601 ggtgcttgcg gaacatcaat ttcttcaata tctgacgcat atgctttctg atattcttct
     6661 ttgagccttg gcattatctt ccaaagctga ataccgccgt agcctactgc agcatagaaa
     6721 tcatccatgg aattatactg ctttttaatc atcagtttct ggaggaaatc gggatattct
     6781 tcttcggaaa gattgatgcc gtggcgcttg aactccttgt caagcatctg cctgccttcg
     6841 gcaatatttt catcacgctt ttcatgctta taccatgagc ggatttttga ttttgccgat
     6901 gcggttttac agatatcaac ccatcccctg tttgggtgtt cctctttctg ggtgataatt
     6961 tcgcagatat cgcctgtttt aagcttatag tcaatcggaa cgatctttcc gttaatttta
     7021 gcaccgacca ttctgtgacc tacctgtgtg tgaataagat aagccatatc aatcggagtt
     7081 gaaccctgcg gaagattgaa cacatcgcct ctcgggctga acacataaac atcgttttcc
     7141 gtaaaatcgt ttctgatatt ctttgcgatg tcggtaacat cttctgcctc aagctgatca
     7201 agaatcatat ccttgacctt tttgatgttc tcctccattt tgtcattatt tttgccgcct
     7261 gcgcccattc caagcttata cttccaatgg gcggcaattc cgtattcggc ggtgtaatgc
     7321 atttcccatg ttcttatctg aatttcaaaa ggaattgcgt ttttgtcgag tacggttgta
     7381 tggagcgact ggtacatatt cggcttaggc attgatatat agtccttgaa acggttagga
     7441 ataggcttga atatatcatg cactacaccg agaacattgt agcaatcgga tactgtgtca
     7501 acgattacac gcacggcaaa aatgtcaaaa atctgatcca ttgttttgcc ctgcatatat
     7561 gttttgcggt aaatactgtt aatactcttt actcgaccgc tgatatacac accggggatt
     7621 gtgccctcgg tttttgcgag aatctgattc ttgatggatt caataaactt gtcacgaccc
     7681 tcttcggtca taagaagatc gtcttcgatt tccttatagc cgattggatc aaggtactga
     7741 agagaacgat cctcaagctc atcttttatg gttttaatac cgagccgatg agcaatcgga
     7801 gcaaacacct gcatattttc aagtgattta tctctccttt tttgttccgg catacagttc
     7861 attgtacgca tattatgaag cctgtcggca agcttgatga taattacacg gatatcatca
     7921 gccattgcga taagcatttt tcttatattt tcagcctgtt gctgttcacg ggttgagtac
     7981 ggaatttttg aaatttttgt aacaccgtca atcagcttgg caacatccgc accgaaaagc
     8041 tttgtaacct cttcaagtgt gactcctgtg tcctctactg tgtcatgaag caatgcaccg
     8101 cagacagaat cggtatccat accgagctct gcaacaatac aggctaccga aacgggatga
     8161 agaatgtacg gaattcccga tactctgcgc tggtcaccgt gagctttttc ggcaaattta
     8221 tatgcctttt cgattttatc catatcgtaa gtgttggcag cagatgcgga aagaatcttt
     8281 ttcagttccg taaaatcctg atagtgtgca acatttgaaa atttacccat cagaatacct
     8341 cccttaattt cttgataatc ggggctgatt cgaggtcaac cctgccgttt acgggcagga
     8401 ctgaaatttt tgaggttttc attccctctg aaatttcgat aagcctgagc tctctcatgg
     8461 cctcaagtat cactttaatt ttaccaaaag acagcctgtt gtcaagcatg tgaaccaaag
     8521 ttgcggtttc aaaagcgtaa ccgccgttgc ttttcagata tctgtaaagc aatgcaaaat
     8581 cgtttctgtc gggcattaaa tccgaaagct gtgaacggtc aagctgtttc cctctgcaaa
     8641 aatcctcata gtttgtacgg ctgtcaagga gcttttcggt atcgcagtcc gaagatttta
     8701 tatctttgac aattacggaa acagacacat ttccgttgaa ctcattgcgg tcaagattta
     8761 ctgcaagatc aagtgtttca ccgattttat acggaaattc ttcggttgat gtaaagaatt
     8821 tcatcgcagt aatctgtatg ttattgcggg acagagtgag tcttaaatgc ctgttcgccg
     8881 aaagcggagt aatgttcttc aatgtcatat tgtacaaacc gaaaacaggc gtcggattgc
     8941 cggcaccgta gggctgcata agcgacagag aatcaatcaa atcaaggttt atgtatgccg
     9001 gattgagctt gcactcaatg ttgattttat catacggcat cttgccgaaa ttgtcggcaa
     9061 attcgtttat tttcctgcga aaagcaggga tattctcaga atcaagagaa agcccgacag
     9121 ccataggatg accgcctctg tgtgtgagaa gatccgaaca ggcaaaaaca gcgtcacaaa
     9181 gaggaaatcc ctcaacactt ctgcccgagc cctttgcaat atcaccgagc tttgaaatga
     9241 cgattgccgg cttgccgtaa gcctctttga ctttggcggc aataagtccg attacgccct
     9301 gatgccagtt ttcaccgtca attactatga ttttatcatt gacaagcgac ggttttctgc
     9361 ggattattcc gtcaattttt tcaagaatat cacgctcaat ctcttgcctt tcggagttgt
     9421 caacgcagag ttgcgatgaa atttcagccg cctcatcata atcttccgta agaagaagcg
     9481 acaccgattt ttcggacaaa ccgagtcgtc ctactgcatt tattctcgga acaattgtaa
     9541 atgacacatt tgttgaagat acggtttttc ccgtaagacc ggaatttttg ataagcgcct
     9601 gcagtccggg acggtcggta tttgtaatgc tttcaagtcc gtgtttgaca aatactcggt
     9661 tttcatcctt caactcaaca acatcaccga ttgtgccgat gctcaaaagg tcggaatagt
     9721 tgtcaagcaa ggtggttgta tcgcaatatt cgccttcaag cgccataatc agcttgaagg
     9781 caacacccac ccccgaaaga tttttgaatc tgcttttaca gtcctctctg tgcaagtcaa
     9841 ctacggcaca agcctttggc aactcgcctg agggcatatg gtggtctgtg accacggtat
     9901 caattccgag tgagcttgcg tatgcaacct cctgtgctgc cgcaattccg ttatcaaccg
     9961 taattatgag ttttattcca cgctcattga gtttgtcaac agcgtttttg ttcattccgt
    10021 agccctcggt ttcacgggac ggaatatagt acattgcgtt tgcgtcaatt gtttcaaggt
    10081 aagaatataa aagtgctgtt gaggtgacac catcggcatc ataatcaccg taaacacaaa
    10141 tcggttcacc gcaatcaagc gccttgttaa tgcgttcaac agccttatcc atatctttta
    10201 tttcaaacgg atcggcaatg aaagaatcgt tgtaaagaaa gccttcaatt tcatctttcg
    10261 ttgtaatatt ccgtatctga agcagcattg caacaattgc aggcagctca tagcgggact
    10321 gaatctcccc cgccttagtt ttatcaagtt ttgcaaccga ccacagcttc aaaatgccgc
    10381 tccctttcgt ttatatagtt gatttatatt agttattata cttttcaagc attgattctg
    10441 cgtgtttaag tgcggcgtcg gttatatcaa caccgccgat tattcgtgca agttcgcctg
    10501 ctctgccgtt atggtcaagg agggttacat ttgtaaatgt tcttcctttt tcaacctgtt
    10561 tgcggataag atagtgataa tccgcaagag ctgcgatttg tgcctgatgt gtcacgcaga
    10621 gaacctgact gcttttgctg acttcacgca gttttaagcc gaccttttct gccgctgaac
    10681 ccgaaatacc tgtgtcaacc tcatcaaaaa tgagggtatc aaccgtatcc gtgcttgcaa
    10741 gaactgtttt gattgcaagc atcattcttg aaagctcacc gcccgatgcg atttttgcaa
    10801 cagggcgagg tgtttcaccc gggtttgcgg agatgagcag ttccatttta tccgtaccct
    10861 ttgaatttgg ttcgcacttt tcaaagtacg gaacaagctc aacattcggc atattcaaaa
    10921 atgccatttc ggattttact tttgttgcaa aggtttttgc ggtttttctg cggagagtac
    10981 cgagtttttc ggcagccgca agcatttttt cttcgctttc tctgcatttt tccgcaagct
    11041 cgtcacggtt gcggtcatag ttcataagtt cgtcaagtct tgcctgatat ttttcggcaa
    11101 ggtccgaaag ctcgtctgac ggacaattat attttcttgt aagacgattg ataaggtcaa
    11161 gcctttcttc aacttcttca agcctttgcg gatcggattc aagcgaatca atggcgtcgg
    11221 aaatgctgtc gcaacaatcc tttaaattgt aatatgcatc tgtaagcgta tttgcaattt
    11281 cttcatattc agcattatat gatgacgctt tttgaagggc agttgatgca gaatcgaccg
    11341 attcaagacc gccgccatag ttttcgtcac cgtcaagagc catttttgca tcattgagca
    11401 gagaaaagat tttctcaaag ctcataagcg ctgttctttc gctctcaagc tcctgttctt
    11461 ctccctgctt aacatcggat tctgtgagtt cattcacctg atatgaaagc aaatcaattt
    11521 ctttcaatct ttcactttcg tccgaatcgg ctgaatttaa tttctttttg agaattttat
    11581 attcatcata acatttttta taatcagcca aaagcttttc gcaaccgccg aaacggtcaa
    11641 tataatcaat atgagtttca ggagagaaaa gttcgtagct ttcgtgctga ccgtgaatgt
    11701 ttataaggtg ctttgaaagt tctttgagca ttgaaaccgt tgcaggcttg ccgttgattt
    11761 tacaactgct cttgccgttt ctgttgagag ttctctgcaa aatgagcatt ccctcgtcat
    11821 cggaaaagcc gagttcatca agcttttcaa caatgctttg gttaatatcg tcaaattgag
    11881 cgcttacaaa ggctgaatca gcctcggtgc gaataatttc ctttgatgtc cgctgtccca
    11941 taattgcatt gattgcgtca attattatac ttttacctgc acctgtttca cctgtgagga
    12001 cattaagacc gttttcaaaa tctattgaag ttttttcaat cacggcaatg ttttctatat
    12061 ataaagttct gagcatttca atcagtcctg tcaaataagc tttttgagtt cttcggtaag
    12121 cgacaccgca agatcggctg ttcggcaggc aatgaagatt gtatcgtcac ctgcaattgt
    12181 tccgacaaca gacggatagt cggcagaatc gagtgcggca caaactgcct gtgccatacc
    12241 gctgagggtt tttattacaa caagattctg tgctacatct attgaattta ccgaagtgct
    12301 gaaaagattt gaaaaattgg tacggatatc ggttgaactg cgagatgcag aaacatagcg
    12361 atattttccg tcacttccga gagttttaac aaggcgtaaa tccttgatat ctcttgaaat
    12421 tgtagcctgc gttgccttga aaccttcctc acgaagtcgg gtaagcaact catcctgtgt
    12481 ttcaatggga tgttcggcta taatatcaag aattcgtgcg tgtcgccctg ttttcatcaa
    12541 aaatccctct ctttcagttt ttcattgagc agtttataaa aattgcggtt gtggagtaac
    12601 gcaagtttaa gctttaaatc ggatttgcgg attacaaccc tgtcggttgc aagtatcgga
    12661 acattctttt caccgtcaat tgtcagcacg caacagcatt cttcggggat tttcacatga
    12721 accgaaagct ccgattctcc gctgaaaagt acggaacggg aaaataatga atggggacaa
    12781 acgggagtca taagaataca ttccattttc ggtgcgagga ttggtccgcc tgccgacaat
    12841 gaataagcgg ttgagcctgt cggagttgca aaaagcagac cgtccgctct gtattttgaa
    12901 atttcgtcac cgtcaagcgt caggtgcaaa tcaattgttt ttgaaagctg acctcttgcg
    12961 acaacggcat cgttcactgc aagatagtgc tttgagccgt cctcttttat gacctcaaca
    13021 tcaagcaaaa ttctctcctc tgttgtgtaa tcgcccgtaa taagccgttc aagctgttca
    13081 tattcgtgcg gttcaacatc tgcggcaaaa ccgagtctgc cgacattcac accgataagc
    13141 tgtttatctg cttttgcggc atattttgcg gcatggataa tcgttccgtc accgcctact
    13201 gtaattgcca tatcgcagta ttcaaaaagc tccgtaatat tttttgaata atgaatgtgg
    13261 attcccttaa aatactgact gcattcctca atcatagcaa tttctgcatt tttatcaaga
    13321 agaaaaagag aaatcttttt tgcatattta attgcccttt cttttgaaag attgaccact
    13381 aatgcaaatt tcataaaagc acctgctcat tttttgtcaa gagcggcatg tgaattttca
    13441 acaagctgct cgggtgttac atctgtgtat gactttggat catcactttt gcgaagatgg
    13501 ataaggtatt ctatattgcc ctcgggacct ttgattggcg aatagtcgag attaagcaca
    13561 tcaaagccgt tttcaaggca aaaatcaaat atttttctga caacctcaac atgaacggca
    13621 ggatcacgaa ctacgccctt tttgcccacc ttttccctgc ctgcctcaaa ctgcggtttg
    13681 attaggcaga cagcctgtgc gttttcgctc ataagctgtc ttgcaacagg cagaatcagc
    13741 tttaatgaaa tgaacgaaac atcaaccgag aaaaagtcga tttcatcggg aacctgctcc
    13801 cttgtaacct ttctcatatt ggtgcgttca agattgacaa cacggggatc gttgcgaagc
    13861 ttccaagcaa gctgaccgta accgacatca attgaataca ccttttttgc accgttttgg
    13921 agcatacagt cggtaaagcc gcccgttgac gcaccgatat ccattgttat cttatctttt
    13981 aaatcaaagt caaaattgtc tatggccttt tcaagcttta aaccgccacg gctgacatat
    14041 ttctgtgtgt tgccccttac ttcaatttta acattttcat cataagaagt tccgcacttg
    14101 tcggctttct gattgtcaac ataaacctgt cccgacatta taatcgcctt agccttttca
    14161 cggctgtcgg taaagccctt ttcatataca agaatatcaa gtcttttttt catcctaatt
    14221 cttttccttt aaatttttgg taatggtgtt caccattccg tcatcatcga gtttaagctc
    14281 atgaagagcc tcgctgacag acatctgctt gacaaatttg tcattaatag cctttatatg
    14341 ataatctccg cagaactgtg tgcaacagag taaatcggag aaatttctcg caataccgcc
    14401 gtttttgatg ccctcttcaa aaaaccatac attatcgaaa tctgccgcaa aatcaacggc
    14461 attttcgtca atcggtttaa ttctgcaaag ttttaatatg caaatctcaa tgccctgttt
    14521 tgcaagggtt tccttggctt tgcaggcata agagaaaagt cttccgtagg ttatgagaag
    14581 atttttacaa ttcggatttc cgtaaacatc gtatgaaaga ttttcttcac cgaaatcatc
    14641 gggtctgtaa agctctccgc cacggggata acgcacaaca gcgaggctgt cgcaaatgta
    14701 aattgaggtt gaaagagatt ttctcatacc gtcaaaatat gccggagaaa aaattgttgt
    14761 gttcggaatg gtgttgagta tcgaaacatc aaacacaccc tgatgagttt caccgtcatc
    14821 accaacaatt cccgcacggt caaccccgat tacgaggtga agctttccga gagccgcatc
    14881 atgcaaaagc tggtcatagg ctctctggag aaatgttgaa tacacggcaa aaacaggtcg
    14941 catacccttt gcggcaagtc cgcatccaaa agttactgcg tgttcttcag caataccgac
    15001 atcaaaaaat ctttccttaa aaagccttga aaattcggta agtcccgttc ctcccgacat
    15061 tgcggctgta attgcgcaaa tctttttatc gtcctcggca aattcgcaca tagtctttcc
    15121 gaactcagcc gaaaatccct tgtgacttga aagcggttca cccgaatcaa tatcgaacct
    15181 gcctattccg tgaaatccgc ccggattgtc ctcggcaggc tgatagcctt tgcctttttt
    15241 ggtaacaaca tgaacaagaa cgggagaatt cacctttttt gcacactcaa acgcattttc
    15301 aagctcatca atgttgtgtc cgtcaatagg tccgagatag gtaaaaccga agcagtcaaa
    15361 aagagtgttt ttgtacacaa gatttttaag tttagacttt cctccctgaa gaaatctttt
    15421 cagaggtttt ccgataagcg gaatgtgtaa aagaattttt tccgtaattt tcttgactcg
    15481 catataccac ggctgaattc ttaccgttgt aaggtagcgt gacaatgcgc ctacattcgg
    15541 tgaaatggac attttgttat cgttgagaac aacaataaag ttcttgttaa atctgcctga
    15601 gttgttcatt ccctcaaaag caagtccacc cgtaaatgaa ccgtcaccaa tcacggcaac
    15661 ggtgtagctt tggtcgccca taaggtgttt tgctcttgca attccgtatg cggctgatat
    15721 tgatgtactg ctgtgacctg tgttaaaatc gtcgtatttg ctctcggcac gcttcggaaa
    15781 gcctgaaatt ccgccttttt tgcggattgt gtcaaactgc tcgtagcgtc ccgtcaacag
    15841 cttgtgggta tatgcctgat gtccgacatc ccatacaatg ctgtctttcg gcagattaaa
    15901 gctacgctca agagcaacgg taagttcaac aacaccgagg ttcggtgaaa gatgtcctcc
    15961 gtttacagac acggtttcaa cgagcttttc ccttatttcg gtacacagct tttcaagctg
    16021 ttcgtcatta agctttttga catcatgcgg tgatttaatt ttagaaagca gttcatactc
    16081 acccataatt tatttcctat ccgtttaatt aatatttgca aagaattatt tatttctctt
    16141 tgcaagcttg taagcaaatt cttcaagctc ttttgtatca ccgtcaaagc catgaagtgc
    16201 gttaacagct ttttcggtaa gttcatcaac aagctcacgg cacttttcaa ttccgagaag
    16261 tgaaacatag gttgatttgc tgttttctgc gtcactgcct acaggcttgc cgagttcctc
    16321 atcggaggag gttacatcaa gtatatcgtc ctgaatttgg aacgcaatgc cgatgcattc
    16381 gccaaattca gaggcggctt taatttgctt atcgtttgca cctgcgctta tacagcccag
    16441 ttcacaagcc gctttgataa gacaggctgt ttttttgtag tccatttcac gaaggacttc
    16501 aatcggagcg tttgtgtttt cgctcataag gtcaatcacc tgaccgccta tcattcctgt
    16561 cgcacctgcg tattttgaaa gtacggaaac acctctgcaa cacgcagaag ttccgcagat
    16621 ttttactgtc ttttcgtttg taagggtttc gaaggcaagc gtcagaaggg cgtcacctgc
    16681 aagcaatgcg atatcttcgc cgaatacctt gtggtttgac ggcttgcctc ttctgaggtc
    16741 atcgttgtcc atacacggca aatcatcgtg aatgagagaa tatgtgtgaa tcatttcaac
    16801 tgcacaggca aatgccgttg cagactcttc acttccgccg caaaggctgt taaacgagaa
    16861 cacaagatac ggacggacac gctttccgcc tgcctcaagg ctgtaagcca ttgcgtcaat
    16921 cagctttttt tcacggcaat ccgtactcgg cagacagctc tttaaagcgt tttcaatatt
    16981 ttttgcataa tctttagtca ttgtcatcac cctttgaaag ctttgaaatt ctctcgttta
    17041 tatcaataat tttacccttg cattgttcaa gctgtttcat acaataagca aggagttcac
    17101 acgcttttgc gtactcctcc attgtctgtt cgagagtaag ttcaccgctc tccatttttg
    17161 aaaccgtttt ttcaagcagt tcgtttgcct gctcaaaagt caaattttta ttttccattg
    17221 gtttttacct ctgtacctgc atattcaagc agtctttgca tacggtgatt tgcaccgctt
    17281 cggctgattg gggttgaaag attctgtccc aaatcacgaa gtgacatatc gggattttca
    17341 agccttaaat atgcgatttc acgcaagtcc tcgggcaaag aatcgattcc gactgtcttt
    17401 tttatatgtt cgattgcact gagctgtttt gcagacgccg ttgcgatttt tccgatattc
    17461 gcaacctcgc cgttaattct gcggttcaca ttgttgcgaa cctgctttat tgcctttgta
    17521 cccataattt ccattgatct tatcggtgag cctatgtagg taagcaagtc acaaatctgc
    17581 tcgctctcct taaaatagac aatgtaactg ccgctgcgga caaccgtttt tggaataagg
    17641 gtacattcgg ttatttcgga aagaatcttg cacaaatcct gagaaagatt tttgtgcgga
    17701 acacagaatt caagatgata gcttttcatg ggatcggata ccgaaccgca gctaagaaaa
    17761 acgccacgca aaaacgattg cgaaagttct tcgcttgaaa gatttgccct gttgacacgc
    17821 aaagtaatct gaccgtccgt atgaccgaaa tcttcaaaaa tacgcttgca atcgtttccg
    17881 tcaaccacaa ttattttgta aagatggctg tcgcttgatt ttgttcgcaa agccgattgt
    17941 ttttcaatta tcggcatata taaattctgc aaaagaaata taagacgatt tcccgcataa
    18001 ttgctttctg ttgtgaaggt tattttgttc ggagaaaaat ttctgctgaa taaaagcatt
    18061 ccgtaaagct ccgctttgag agcctcacgg tcaaactgtt caaccgaaca aatttctttt
    18121 tttacatccg aggaaaatga catttctgca ccttacctgt caatatctct gtgctgaata
    18181 acacacctat agcctttttc acgaaagtgg tcgttcagct gcattgcgat tgtaacactt
    18241 ctgtgcttgc cgcccgtaca acctatgccg acaacaagct cgcttttgcc ctctttgaga
    18301 tagagcggta cggaaaaatc gagcatactc aaaagtcgct ttaaaaattc ggttgtttct
    18361 tcactttcaa gaacatagtc acgcacatct ttgtcaagtc ctgtaagatg tttgagttcg
    18421 ggaatgtaaa aaggattagg cagacaacgc acatccataa tgagatctgc ctcaagcgga
    18481 attccgtatt tgaaaccgaa cgacataaat gtcagcgtaa tgctttgtga agtgtcttcc
    18541 atgaacattg acataattcg ctctcggagc tgtttgtttg acatatatgt agtgtcaatg
    18601 ttataatcgg ctgttctttt aataggcaaa agaagctccc tttcaagctc aacggcagac
    18661 agtaccgaac cgtccttgag tttatccgaa agaggatgtc ttcttcttgt ttccttataa
    18721 cgaacaacga gcctgtccgt cattgcgtca aagaacaata cctttacatt ttcgtaatgc
    18781 attttgaagt gttcaatctg agaacacatt tcctcaaatt tttcgttacc tcttacatcg
    18841 acaacaaccg caaccctctt cattgaatca tcttcagatt tgaggcaaag ccgatagagt
    18901 gttgacaaca gacttgacgg aagattgtca acacaataat aaccgatgtc ttcaagcaca
    18961 tgaagagcgc ctgttttgcc tgcacccgac attcccgtaa tgattacaaa ttccataaaa
    19021 ttacaccttt tttgctttat agaaaaataa gttcacattc aagcttgtag cctgtttcat
    19081 tttcaacagt tttctgaatt tccgacacaa gctgttttac atcgtcagcc gtagcctttg
    19141 atttgttgat gataaatcct gcgtgctttt cgctgacctg agcaccgccg actgttttgc
    19201 ccttgagtcc gcattgctca atgagcgcac ctgcaaatgc accctcgggt cttttgaaca
    19261 cactgcctgc gctcggatat tcaagcggct gttttgttga acggcgattc atataatcgt
    19321 tcattctgtt ctgaatttct tcgggatcat cttttttaag tttgaatacc gcacctgtga
    19381 ttatgcaacc gtttttgcgg taaacgcttg ttctgtagcc aaactcaaga ttctccgcct
    19441 ctgttctgcc gatatcaccg ttctgtgtga tgtgagaaac gctgtataca acatctttca
    19501 tttcaccgcc gtatgcacct gcgttcataa acaaagcacc gccgactgtt cccggaattc
    19561 cccatgcaaa ttcaagtccc gaaagtccgc atttttgggc aaacttacac agacttccca
    19621 aagccgcacc tgcgccgcaa tagattgttg tatcgtcaat taatgaaata ttcctgaaat
    19681 caccgtcaag acggagaacc gtgccgtgga ttccctcatc cggaaccaga acattactgc
    19741 cattgccgag aagaatatac ttctccttgc agacatcgca ttcctttaaa attttcatca
    19801 gctgagaaag atttgtcacc tttatgtaaa catctgcttt tccgccgatt ttaaatgaag
    19861 tgtgtctgct catcggttca aatcgtcttg cgtcacaatc aagcttttga gcacagttat
    19921 atataatatc ggtagtgtcc attcaattac cctccgttgt tagtttagaa tatgaaattt
    19981 ctgtttcctg tattccgtta caaagcggaa ttattcccat gtatcaacaa taacctttgg
    20041 tgacggcgga agatcctcaa cagccattcc gagatttttg agaagttctc ttgcggcatt
    20101 gtagcccatt ttcttctgtc tgttattcat ggcggcaacc tctatgatta cggcaaggtt
    20161 acggccgggc tttacgggaa ttgtaagagt aggcacttcg acaccgagaa tcttcatgta
    20221 ttcgttgtca agtcccattc tgtcataaac ctttgcgctg tcccattgtt caaggttgat
    20281 taccatatca atcttttcct gcagcttgat agcgcccata ccgaaaagtt ttctggcatt
    20341 gataattccg atacctcgaa gctcaatgaa gtgacggata ttttcgggcg actgaccata
    20401 gagtgtagtc tgggcagttc ttctgatttc tacggcatca tcggcaacaa gacggtgacc
    20461 acgcttgata agctcaattg cggtttcact cttaccgacg ccgctgtcgc ccgtaagcag
    20521 acagccttcg ccgtacactt caacaagaac gccgtgacgg gtgattctcg gagcaagctc
    20581 tttgttaaga tattgtacaa gtgacgcagt aagatccgat gtactctgcg gggtgctgaa
    20641 aatagacacc ttgtgcagct ttgcactttc gagaattgcg tttgacggtt caatatcacg
    20701 gcagattatt acagccggag gaccaaagct gaaaatggag tcaataacca ttttctgagc
    20761 ctccggaccg tatctgccga gatatgagaa ctctgtatta ccgagaacct gaattcgctt
    20821 gttgtcaaag aattcaagat aacccgtaag ctcaagtccc ggtctgttga tttcaacagt
    20881 cgatatgtta gtttccttgt aattttcagc gacataaact tcagtgaggt tcaatgcttc
    20941 tgcaatttgc gacagcggaa cagaaaattc aacagacata aatcatcgtc ctatcctaaa
    21001 tatttattac ctttcacaaa tcagagcaac cgctctgccg cctaacaggc agatgtatgc
    21061 tctcaggcta tatacttatt gctattatac accataacgc agttttgata aagtcttttt
    21121 gtgaattatt tttcaatttt taataaaatt ttctcatttt tctgttactg ttttaacaat
    21181 gtctgctttt aattctttat cggaactaat ataacaaata ctcaaatagc cgttgttttt
    21241 acccttaaaa ttattgaata tggataaaga tgttacggaa aggttgttat tattattctt
    21301 caacagaaca atatgttctt taactttttt cagtgcgttt ttatcttttg aaaaaagttg
    21361 caaagttttt gtatccgaaa gggcagtata ggtcaaaggt gaaattgcgg tttcttttga
    21421 aatatagtca atatcggttt tgagagtttt ttctccgaga tttgagtcga taagatacaa
    21481 ttcaagcttt gaaagcgtca gcgcagggat ataggctttc tttgccgata ttacggcttt
    21541 ttcaaggctg tcagcctcaa ccgaaacact ttgcacatct tcgctgttct caagcagata
    21601 gaaattatac acggctttgc ccgatttgtt ttctgcagta atcgtctgaa caagcgaaga
    21661 agtatcaatt cggtttgaat ttgaacagcc gcctaaaatc agaacaattg ccataatcgg
    21721 cagtaaaaat aattttttat tcataaagct ttctccgata tacaaaaaga tagattatag
    21781 gaatcagaag tgcggaaata aaattgagta cagtcagaaa aatattgttc agcaataatt
    21841 cttttacagc attataacta tctgcacaaa caaacaaaac aaacattata aaagctatta
    21901 tcgaaacaaa aacaagcgtt ctttttgaag atgtatttac aatgcagttt gaaacgctga
    21961 tgaggacagc cgttacaaaa acgaaaatac aggcagttac tgcggcaagg taaaagctgt
    22021 caaagccttt catatcacca aaatatgcgg tttttgacaa tagctgaaca gaaaacggtt
    22081 gctgtgcaga atatgcaccg ataacaaaat cggtaaaaaa caaagtcagc attaatgtgc
    22141 cgaatataat aagagttgta attacaggtt gttttattga cagatttttg gtgcttgtgg
    22201 aattgaatcc aaaaataaca ggcagaaacg caaagattaa acagtctgca acattgctac
    22261 gaatatttga aaaatcgggt gtaacaaaag tactgttcgg tgcaaattca aaagatttta
    22321 tatttccgaa aaatatcacg gcaaaagcaa ttatgagaaa tgcaagcaca aaaatgctga
    22381 ctcttgaaag cggatttatc ccctttgacg caccgtaaac acaaatgcaa agtatcagaa
    22441 aggcaatcac ctttgtgtcg acatctggat taactcccga aacaaacata tcgcagaatc
    22501 taatcagaaa agaaacggtt atcacggtga aatacataca ataatatgaa gaaacaaaga
    22561 aaaagcccgt ttgcgatgtg ttttttgcaa aggctgagaa atcaagtctt gttctgtgct
    22621 ttatcgaata aaaaggaaca aagcacaaca gcgtgaaaaa tattccgaat ccgattgacg
    22681 caagacttga aaaaatattg tcgctctgca aaaagctccg ccccattaac aatatcggaa
    22741 gtacagaaca tacaaacagc gaaagcataa gtctttttga cggaaaagtt acattcgatt
    22801 gcattacatc tcctccgttt cggggaattt ctgaaccttg attgtgtgct ttgaaagcct
    22861 tttccaatct gctctgacaa agacatcacg catactccgc cacgagaacg gtgcaagcga
    22921 tgacatatac ggcacgccga ctgatgtttt tgaacacata ttgacaagca ctccgcaggt
    22981 gagcagaact attccccaca agccgaacat tccgcctgcg ataacgcaga aaaatctcaa
    23041 caccataata ggcggataga gtgcgaatgt gacataactg caaattgccg cagttgcaac
    23101 aaccataaga gttgacgagc tgattatgcc tgcgctgacg gcactttcac cgattacaag
    23161 cgctccgaca atgctgacag cgtgtccgag cggctttgga atacgcagtc cggcctctct
    23221 catgacttca tagataaagg ttatgagaat aacttcaagt gttacgggta agggtgtttg
    23281 cgacaaagaa tcggatgttt tcacgagcaa tccggtcgga aaatattcgg gatgaaactg
    23341 tgcaaaagcc gtgtatattc ccggcaaaaa tacggctata aggaacgaaa tatactttaa
    23401 aatccgtatg aatgccgcat aatacgggcg gtttgaataa tcatcaacgc tttgaaattc
    23461 ttctgcaaaa aggtgcggaa tgattatcac cgacggtgtt ccgtcaatca gaattgccac
    23521 tctgccctcc gacagctttc cgcagaccgt atccggtctt tcggaaatcc cgacacccga
    23581 aaaaagtgat tttgaattgt tgtcttcaag ataactgctt aaatatcccg acgcaagaat
    23641 cattttaagg ttgcagtttt caagccgttc tctgattgct ttaagaattt gctttgacac
    23701 gctgttttga agataacaaa tcatcatctg cgtgtttgaa ctgtatccgc ttgttatggt
    23761 ttcaaaaaca aggtcgggac ttttgattct tcgtcttatt aaagtcatat tgattctcag
    23821 cggttctgta aaaccttctc tgcagcctct ctgcactacc tcactttcgg gttccgaaac
    23881 acttctgaat gaaaagccct gtgctccgat tgcaagcata cgactgcaac cgtcaacaac
    23941 aagcagggca aaacctgatg ttgaaaagct gattgcctcg tcaattgtcg tgaactcgac
    24001 aatttcactt gatgtcagca cggtattttt gataaggtca aaaaccttat cgggggattg
    24061 gcagtcaaaa taataatcga gcagaggatt gattaccgac aatgcaagcg tgtcctttcc
    24121 gcacattccc tcaattgtga ttattgcgga ttttatactg cattgtccct ttagattcat
    24181 ttcacgaacc gtaaagtctg ccgaattttt gaacattttc ttcagagttg aaatattttc
    24241 ttttaatgaa ttatacattt aatcaccgtc ttaatatatc tgctttttat tttgcggtat
    24301 atcggctcaa attatgcaaa tattagtaac ggtgatgtta tgttaatttc aataatcaga
    24361 acaattttgc tctatgcctt tgtcattctg gcagtcaggt taatgggaaa gcgacagatg
    24421 agcgatatgc agccgagcga gcttgtaatc acaatgatag tgtcggatat cgcatcaatt
    24481 cctatgcaga acacatctca accgctgttg agcggagtta ttcccgttgc aattctcgtt
    24541 gcacttgaaa ttaccgcaag cattattatg atgaagtgga gcggtttcag aagtcttgtg
    24601 tgcggaaaac ccgttatagt aattaaagac ggaaggattc tgcaatcggc tatgaaaaag
    24661 ctgcggctta ctacagaaga tttgagcgtc agattaagac aacagggtat tttttcaatc
    24721 gaggatgttc aatactgcat tgttgaaaca aacggtgagg tgagcgttct tgaaaagcct
    24781 gagaagagaa agccttctgc cgaggatttc gggttaaaaa ttaaggacaa taaaattgaa
    24841 accgtagttg tcagcaacgg aaaaattctt gaaaattcgc ttaatttatg tgaaaaagaa
    24901 aagggttatg ttaaaaatat tctgcaaaag aaaaatatta agcagagcaa tgtttttctg
    24961 atgactcttg acgcactcgg aaattacaac attataaaga aggaataaca atatgaaaag
    25021 aatttacatt gcactttcac ttgttctggc ggcactatgc tttgcatctg ttgaaacagg
    25081 atttatttcg ggcaaaactg attcattcat tgcaaaaatt gaatgtgccg acagactgat
    25141 gagcagactt gaatttgaag atgcgctcaa aatatgtgaa gaaaccgaga aagaatggta
    25201 tgacaacgca aaattgattg acaccatgct cattcacgat tatgttgaca atatcggatt
    25261 aagcatttcg agaatgaccg tatttgcaca aaa
//
LOCUS       DAOMVE010000009        24821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_317_length_24821_cov_2.918719, whole genome shotgun sequence.
ACCESSION   DAOMVE010000009 DAOMVE010000000
VERSION     DAOMVE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 24821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24821
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_317_length_24821_cov_2.918719"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1400)
                     /gene="lysA"
                     /locus_tag="PLS64_01115"
     CDS             complement(102..1400)
                     /gene="lysA"
                     /locus_tag="PLS64_01115"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRL42465.1"
                     /translation="MFVSECLNVNNKGHLTIGGCDTLELAKEFGTPLYVLDETTIRNV
                     CKAYVKSFEKYYHGNGMPLYASKALSCKELCRIAKEEGLGLDVVSGGEIYTAVQAGFP
                     MEKVHFHGNNKTADEIRFALESNVGKFVVDNLYELELLNEICGEMGKKANISFRIKPG
                     VDAHTHNFIRTGQIDSKFGFALETGEAFEAVKKAQMYDNVNLTELHCHIGSQIFDIDP
                     FVTAAEIMLDFMGKIHDELGIVISELNLGGGFGIMYTKDDEPVPYENYMEKVSEAVKA
                     KAKEHGLPVPYIFIEPGRSIVGEAGITLYKVGGKKEIPNVRTYVSVDGGMTDNIRYAL
                     YQSAYTVVNAGKADKEPDEIVTVAGKCCESGDLVQEHTKVAKVEVGDTLAVLSTGAYN
                     YSMASNYNRIPRPAIVMVKDGKARVVVKRESYEDIVKNDI"
     gene            complement(1406..2008)
                     /gene="yihA"
                     /locus_tag="PLS64_01120"
     CDS             complement(1406..2008)
                     /gene="yihA"
                     /locus_tag="PLS64_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRL42466.1"
                     /translation="MNFNEVAFEFAAGRVDQLPESDMPEIVFSGHSNVGKSSLINKLV
                     QRKALARVSAQPGKTATINFYKLKEFRMVDLPGYGYAKVSKAEKDRWAKLVEGYFAQD
                     RQRPLVIQILDLRHPPTDDDLHMIDFLYRGGFNFAAVLTKSDKLKKTAFEKQIEYYKD
                     IFSTIEHVPLIPFSAQTGFGNDEIRKLIETSIANFKNTEV"
     gene            complement(2023..4434)
                     /gene="lon"
                     /locus_tag="PLS64_01125"
     CDS             complement(2023..4434)
                     /gene="lon"
                     /locus_tag="PLS64_01125"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HRL42467.1"
                     /translation="MDFDMQKNLTLPVLPLRGLVVFPKSLIHFDVGRKKSITAINKAM
                     KADQLVFLTSQKDAAINEPDIFDVYDTGVIAKVVQVLKQPENTTRIVIEGQCRATIIN
                     PVFDEKCLVAEVKPYEEESEYLTARDSALMRTVKNEFDKYLEISPKMPSDIIFKVALC
                     KRPGELADFITANLILDYRVKQSILETFPESERLESVLDVLVNENFVLKLEDEISQKA
                     KMRIDENQRDYFLREQKRAIEEELGEDDSPIDDAENYVDKIEKLNLDEKSADILYKEC
                     KKLSKMPYGSQEAAVIRTYLDTVLDLPWNKSSKDKIVISKVQKALDKTHYGLDKVKKR
                     IIEQLAVRVLSEKQKGQIICLAGPPGVGKTSIAQSIAKAIGRKSERIALGGVRDEAEI
                     RGHRKTYIGSMPGRIINALQHAGTNNPVLILDEIDKLAADYKGDPTSALLEVLDIEQN
                     NKFVDHYIELPFDLSNVMFITTANDISAIPAPLRDRMEIIEISSYTREEKFHIAKKHL
                     IPKQLDECGFTSKELKFTQKAIYALIDFYTREAGVRTLERLIASVMRKCAVEKLTEGT
                     EVFKIDEKEVERFLGHKKFIPDSLAKMDEIGIVNGLAWTSVGGEILPIEVAVMEGTGK
                     IELTGSLGDVMQESAKTAITCIRSHAAVLGIDSDFYKNKDIHIHAPEGAVPKDGPSAG
                     ITMATAIYSALTLKPARHDIAMTGEITLRGNVLPIGGLKEKSMAAFKNGITTVIIPKD
                     NEPDLEDVDKAVLEKVKFIPVRNFSEVVALAVNNTVRITDNQWNGIDMTAVRSATKTV
                     NAVKQ"
     gene            complement(4499..5839)
                     /gene="clpX"
                     /locus_tag="PLS64_01130"
     CDS             complement(4499..5839)
                     /gene="clpX"
                     /locus_tag="PLS64_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRL42468.1"
                     /translation="MANKNNEDIYCSFCGKPQEMAGRLIAGNGVYICDRCVELCMNIL
                     RESGELERENENKLSKSGEPQTPELSVDELLKPKEIKAILDEYVIGQDHAKVTLSVAV
                     YNHYKRAFSNDESVDFAKSNVLLLGPTGVGKTLLAQTLAKALDVPFAIADATTLTEAG
                     YVGEDVENILLKLIQAADFDIEKAEHGIIYIDEIDKIARKSENPSITRDVSGEGVQQA
                     LLKIVEGTVSNVPPQGGRKHPQQEYLQIDTKNILFICGGAFDGLEKIVEKRKGSSVIG
                     FESLVQSKQELDSTDWMKEVTAHDLVKYGIIPELIGRLPVITALSGLDTDALVKILTV
                     PKNSIVQQYKKLFELDGVELLFEEDALRAIAQQAQDQHTGARGLRGIMEDILTDLMFE
                     APSDPTIDKIIITEDVIKNGAAPQITRKKELLQTNSVKLTQKDAKKRQKRSTAS"
     gene            complement(5855..6436)
                     /gene="clpP"
                     /locus_tag="PLS64_01135"
     CDS             complement(5855..6436)
                     /gene="clpP"
                     /locus_tag="PLS64_01135"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRL42469.1"
                     /translation="MALVPYVIEQSSRGERSFDIYSRLLEDRIVMLNEEVNSASASVV
                     VAQLLYLESKDPTKDISLYINSPGGSVTDGFAIFDTMNYIKCDVSTICMGMAASMGAF
                     LLAAGAKGKRLALPNSDIMIHQPSGGAQGQATDMEIHTRHILDMKKRLNQILADNTGQ
                     PIDVIAKDTNRDNFMTAQQALEYGLIDKVIEKR"
     gene            complement(6491..7771)
                     /gene="tig"
                     /locus_tag="PLS64_01140"
     CDS             complement(6491..7771)
                     /gene="tig"
                     /locus_tag="PLS64_01140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRL42470.1"
                     /translation="MALKECTKKEQANSYELEVNVDGETFGNAVNRVYKKQVKSINIP
                     GFRKGKAPRHVIEKMYGSEVFYEDAMQDCYPDALYEAAKEAGIKIVSVETLEAIEASK
                     EGFSFKANVVVEPEMEINNYLGIEVTKKSTEVTDELVDEEIEKVRDRNSRMVTVDDRA
                     AQNDDVAVIDFEGFVDGEAFEGGKAENYNLKLGSGNFIPGFEEQIVGHKTGEEFTITV
                     KFPDDYQAEELKGKEAEFKINLHEIKAKELPEVDDEFVKDVSDKETLDEYKAELKETV
                     AKRLQDEADKDVDNQISEKLMELLEGEIPEAMYDNQANEMVREFDMRLRSQGLDMKTY
                     MQYMGMDANALKGMYKEEAEKRVKLRLALEAVARKENIEVTEADLDAEYGKMAEAYKM
                     DVDKVKEAVPAESLTEDVKVEKALNLVKDKAVIK"
     gene            complement(7862..8551)
                     /locus_tag="PLS64_01145"
     CDS             complement(7862..8551)
                     /locus_tag="PLS64_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRL42471.1"
                     /translation="MALFAIADLHLSLGEDKPMDVFAGWNDYTSRLENNWRKLVTDND
                     TVVVSGDISWAMKLEETLTDFTFIENLPGKKIFNKGNHDYWWSTKTKMDVFLKNNSLD
                     SISILFNNSYVVDDYAICGTRGWFLENESQNDIKVLNREVGRLEMSIQSAIKTGKEPI
                     VFLHYPPVYGTTKCDEIFNVLLKYDIKKCYYGHLHGKKNMRFAIEGEYEGIDFKLISC
                     DRLSFMPILVR"
     gene            complement(8551..10059)
                     /gene="thrC"
                     /locus_tag="PLS64_01150"
     CDS             complement(8551..10059)
                     /gene="thrC"
                     /locus_tag="PLS64_01150"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRL42472.1"
                     /translation="MLYNSTQNAKEVVSAAQAIAQGISKDGGLFVPQEFPKYDENTFK
                     ALLKTDYKGRAKKVFSDFLSDFTEEEISDCVEKAYTAQKFGGENPAPISYCNLDGTTL
                     NILELWHGPTCAFKDMALQILPHLLTKSLKKKYDGKTAVILVATSGDTGKAALEGFKD
                     VEHTKIVVFYPVDGVSPMQKHQMNTQEGENVRVCAIKGNFDDAQTGVKKIFTTPSVAE
                     KLAENNMLFSSANSINWGRLLPQIVYYISAYCDLVNDGKIELGDKINVVVPTGNFGNI
                     LASYYASLMGLPINKFICASNSNNVLTDFIKTGVYDKNRNFYTTISPSMDILVSSNLE
                     RLLYKLSGDNDEVTRGWMNALKSEGKYEVSDDVKAVINEKFYGGFCDDKNTEATIHEM
                     FEQDKYLCDTHTAVAINVYKQYVEETGDKTPSVIASTASPYKFSKAVLEAVEPNATLP
                     ETEFDMVDELHKVSGAEVPAPLANLKNKTARFSDVTEVNDMENYVLGALGID"
     gene            complement(10102..11040)
                     /locus_tag="PLS64_01155"
     CDS             complement(10102..11040)
                     /locus_tag="PLS64_01155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HRL42473.1"
                     /translation="MNFFDLHCDTLYKAVTEKSELDNPSYEVKLNNNSKSHRLQCYAI
                     WLPDTLDSNEAEKLFFESADYLKSECNRLGIELLGIGEFTDNAFSKYRNSAFFTVENG
                     KALNGKIENVKRFAELGVRIMTLTWNEMNEIGSGVLSEDKCGLTDFGKLAVAEMEKYG
                     IVIDISHASDELFYDVVNQTNKPFIATHSDSRTITQNPRNLTDEQIKIIIQRGGLIGL
                     NLHNAFLNNNPDKACMNDVLKHCEYMLSLGCENSLCFGTDFDGCDLPRDIVGSDSIGE
                     IYELFLRNNYNESVLKKIFYENAYNFFENFDNQRIM"
     gene            complement(11053..11415)
                     /locus_tag="PLS64_01160"
     CDS             complement(11053..11415)
                     /locus_tag="PLS64_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRL42474.1"
                     /translation="MIFTTKQTGDKGEAYAAKYLKKHKYKIIARNYKKPYGEIDIIAE
                     NKEVIAFVEVKTRKENSLTQPVDAVNRKKQMKILKTADMYMYENNIDKECRFDVCEVF
                     VNSDNLKLVGINYIEDAF"
     gene            complement(11412..12017)
                     /locus_tag="PLS64_01165"
     CDS             complement(11412..12017)
                     /locus_tag="PLS64_01165"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRL42475.1"
                     /translation="MDWLEYENKNIARGFLNICGVDEAGRGPLAGPVCAAAVILPENT
                     VIEGVNDSKKLSEKKREALFDVIKEQALAYSIAFASVEEIEKMNILNAAMLAMKRAVE
                     GLEPRADYAMIDGNRMPPLSIEGECIVKGDAKSMSIACASILAKVSRDRLLYEYDKEF
                     PQYLFAKHKGYGTKAHIEAILKYGPCKYHRPSFLKKIYNKK"
     gene            complement(12020..12891)
                     /gene="ylqF"
                     /locus_tag="PLS64_01170"
                     /pseudo
     CDS             complement(12020..12891)
                     /gene="ylqF"
                     /locus_tag="PLS64_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523819.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
     gene            complement(12920..13495)
                     /gene="lepB"
                     /locus_tag="PLS64_01175"
     CDS             complement(12920..13495)
                     /gene="lepB"
                     /locus_tag="PLS64_01175"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRL42476.1"
                     /translation="MPQKHKNNKIRIKINDKKITDYMFDFTRTIIFVFAIISVAFTFI
                     IRDANVVGNSMLDTLHSDDKIFITNFMYEPKCGDIVAINAENQIEKRIIKRVIAVEGQ
                     TLVVDYGKNAVYVDGIKIDEPYVSSLTREPSNPLQIPYVIPEGYIFVMGDNRIISLDS
                     RDKSIGLVSVDDVIGKAQFIFFPLDRFKYLY"
     gene            complement(13600..14277)
                     /gene="lepB"
                     /locus_tag="PLS64_01180"
     CDS             complement(13600..14277)
                     /gene="lepB"
                     /locus_tag="PLS64_01180"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRL42477.1"
                     /translation="MKKSKNNVNEANMAVLQPEGVNDSSDINPEMYVDKKPSISEKQF
                     LVAKEEGAMSGFYDWVRCILFAISVVVVCLIFLFRLVDVEGRSMNNTLATNDKVIVTN
                     LFYTPKNNDIVVISHGAEYNSPIIKRVIAVAGQTVKVDYDNDQIIVDGVVLDEPYIEG
                     STYGNNIGDNEIPEVIPEGKIFVMGDNRKDSMDSRSTKIGLIDVDDVIGKAQFVVYPF
                     DHFGYLY"
     gene            complement(14347..14694)
                     /gene="rplS"
                     /locus_tag="PLS64_01185"
     CDS             complement(14347..14694)
                     /gene="rplS"
                     /locus_tag="PLS64_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRL42478.1"
                     /translation="MDALKTIAAASVKETTPEFSVGDTVKVSVNIREGERERIQMFEG
                     TVIAKRGSGVAETFTVRRVAYGVGVERVFPLHSPNVKDVQVVRYGKVRRSKLYYLRDR
                     VGKAAKVKEQIRK"
     gene            complement(14899..15819)
                     /locus_tag="PLS64_01190"
     CDS             complement(14899..15819)
                     /locus_tag="PLS64_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRL42479.1"
                     /translation="MNEFKLNCETSGVRLDKFISDANIDLSRSAAVNLIENGAVLVND
                     SVVSKKYKLSQGDIVVVNVPDPTPYEAKAENIPLDIVYEDECLLVVNKPKGMVVHPAA
                     GNYDGTLVNALLYHCGDSLSGINGVLRPGIVHRIDKDTSGLLIVAKNDFTHRSLAEQI
                     KEHSFTREYESIVFGNLKDDEGTVDAPIGRNPKDRKKMCVIEKNSKNAVTHYSVITRY
                     KGYTHIKCKLETGRTHQIRVHMAYIGHPVSGDKVYGVKNEKVDFEGQCLHARKIGFIH
                     PKTNEYIEFTSELPDYFKKYLKKLENISSH"
     gene            complement(15812..16306)
                     /gene="lspA"
                     /locus_tag="PLS64_01195"
     CDS             complement(15812..16306)
                     /gene="lspA"
                     /locus_tag="PLS64_01195"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRL42480.1"
                     /translation="MEDKMPVIALIIGVLLAATDQIIKYFVLTYLQPVGSVTAIDGLL
                     DFTYVENRGVAFGMFSDMRWFFVAVTAVLIAIIIYIMFKKKPSGKMFYISAALIIGGG
                     IGNLIDRIFYGFVVDYISVSFFPPVCNFADYCITVGTLLLIVYVLFFSNVMKSEKKVN
                     KSNE"
     gene            complement(16357..19122)
                     /gene="ileS"
                     /locus_tag="PLS64_01200"
     CDS             complement(16357..19122)
                     /gene="ileS"
                     /locus_tag="PLS64_01200"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRL42481.1"
                     /translation="MSQDYNSTLNLPKTDFPMRAGLPKSEPVTLQNWEDEKVYENLMK
                     VNEGKPLFILHDGPPYANGDIHLGHALNKILKDFIVRYKNMAGFKAPYVPGWDTHGLP
                     TELKARKKAGIGNSAEISVVELRKMCEEFVKGYINDQRTQFKRLGVIGEWDKPYITLN
                     HEFEAEQIRVFAEMATKGYIYKGLKPVYWCPECKTALAEAEIEYAEDPCHSIYVKFNV
                     TDDKGVFSNMGIDPSKVKFVIWTTTTWTLPANVAICVGPRFEYSVIKTGDEYLVMASE
                     LYKSALDEAGITDFEVVATVKGSELEYIKTAHPFLDRESLVIVGEHVTLESGTGCVHT
                     APGHGVDDYNVCQNYPEIPIICPVDSNGVLTEEAGQFAGLTTDEANKKIAAYLEENGS
                     LFALKKIVHQYPHCWRCKTPILFRATDQWFCSVDDFKDEAVDAINTVKWIPAWGKDRI
                     TSMVRERKDWCISRQRKWGVPIPIFFCKDCGEALIDKDAMLAVADLFGKEGSNAWYNH
                     TAAEILPAGTKCKKCGCTEFDKEKDIMDVWFDSGSSHAAVLEQRDNLESPADLYLEGN
                     DQYRGWFQSSLLTSVATRGRAPYKAVLTHGMILDDESRKMSKSLGNGISPQDVIKQYG
                     ADVLRLWVASTDYQTDVHISKDILKQISESYRKIRNTARYILGNISDFNPDTDMVDFD
                     KVLPIDKWALAKLNDLITKVRTSYDNYEFHIVYHSIHNFCVVDMSNFYLDVLKDRLYT
                     EKADSLSRRAAQTVIYVILDAMTRMIAPLLAYTSDEIWKYMPHSSNENAEHVIFNEIP
                     ENIAIDVDDKFMAFWDRIHELRDNVKKSLESLIKDKTIKGSLEAKVTLCAGGETLEFL
                     KKAEPELCAAFIVSEVEIVDNGGELEIKPEKAEGEKCERCWAISKTVGQCAEHPTICF
                     RCVQNLK"
     gene            complement(19160..19834)
                     /locus_tag="PLS64_01205"
     CDS             complement(19160..19834)
                     /locus_tag="PLS64_01205"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HRL42482.1"
                     /translation="MLTIDEIKNISFRKATLNGGYRAEDVDSFIDEVIVSFEQLKKEK
                     TNLVHKIDVLATRVEQYRADEETVRNALLASQKVADACIREAKEKAAKIVRDAETKAQ
                     ALLVDANNMTAAEKEHYLQLQSDAANLRNELISLYSKHIKSIDDLPSTSDVEASKAEL
                     DEKYPTEEVTAPAQPQVEDDVKIAVPSEMKAEPIAETEEVSAHPKRQSKFSHLKFGDN
                     YDVTAD"
     gene            complement(19848..20615)
                     /locus_tag="PLS64_01210"
     CDS             complement(19848..20615)
                     /locus_tag="PLS64_01210"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HRL42483.1"
                     /translation="MSLRTTVFTSDDDKQLIARLNNGIDLCLTRQKPYFMPFMSERKQ
                     ALLVSELKKAYFDNYLFFGGYDDSERKMLGLFFDEPSTSMFPICGIEFSFRKCDRLTH
                     RDFLGSLMSLGIERETVGDILVEDGRAVVFVKAELSDYVKSQISKVGRVGVKVDDADL
                     SKLPQGRGVEEKTFIVSSLRLDNIVAAVCKLSREKTKTVIMSDSVCVNFEETKNVSFN
                     LKENDVFTIRGKGKFVLKGILGTTGKGRMRISVIHYK"
     gene            complement(20581..21048)
                     /gene="sepF"
                     /locus_tag="PLS64_01215"
     CDS             complement(20581..21048)
                     /gene="sepF"
                     /locus_tag="PLS64_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HRL42484.1"
                     /translation="MAGFVDKFKRMWDAPDDEYEYDEYGYADEGAEEDYKEEPVRNTK
                     KERDLGGGNKVVNINATTNLQVGLFKPERFGEETRTIADELMKAHTVVLNLEDTNKDM
                     ARRILDFLSGVAYANRGKIKRVATNTYIIIPSNVDLTGDDLLDELENNGVYFG"
     gene            complement(21067..21420)
                     /locus_tag="PLS64_01220"
     CDS             complement(21067..21420)
                     /locus_tag="PLS64_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRL42485.1"
                     /translation="MNKNCDVLVEVNIGREENKSGVMPEQLEELLCQIAELDGISVKG
                     LMAIPPICESSQKISEFFNKMHNIFIDISQKKLDNIDMNILSMGMSADYYEAILAGAN
                     MVRVGSALFGARNYT"
     gene            complement(21423..21746)
                     /locus_tag="PLS64_01225"
     CDS             complement(21423..21746)
                     /locus_tag="PLS64_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42486.1"
                     /translation="MTEKLLTDVEYNYKFIDERIAEAAEKSGRKREDITFLAATKMVE
                     PEVINHAISLGLDHIGENKVQELLSKYDKYNLDNCSLQFIGHLQTNKVRQIIKTPHRK
                     FLNRL"
     gene            complement(21724..23007)
                     /locus_tag="PLS64_01230"
     CDS             complement(21724..23007)
                     /locus_tag="PLS64_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRL42487.1"
                     /translation="MDKLLFRRILMIALSVLAVVYAAYLLISTNFKMYPTENAVQTTV
                     TDKIYSNGFIIRDENMIQNNTSGVLSYTVHDGDEVKAGGEIAKSYANDDDAASQSKIS
                     ALQEQLSDLQTLQKTSTAGNIGIDTINNNINNNIISQIRSINDGDLANIDNVTNNLLY
                     SINQRQIYTGKATNFNDRISELQAEIATLEGKTGKSTGNITTEKSGCFLSHCDGYENA
                     LDYNNLDRLTLSDLDNVKQTKVSGNIAGKVVTGLKWYVACKVTADEATRLSLWDGSAT
                     VLFSDASSESIPATIKRIYQESKDSDALLILECDYMNSDLAQARQEPIEIGLGSYTGL
                     RISKKAIHDDYVTKTTYDDNDNKHTEQVKVQGVYVLHGSEVRFKQISILYADDDYVIC
                     DPEPDSKDLVDGTTVELYDQVILEGDDLYDGKVVN"
     gene            complement(23000..23950)
                     /gene="miaA"
                     /locus_tag="PLS64_01235"
     CDS             complement(23000..23950)
                     /gene="miaA"
                     /locus_tag="PLS64_01235"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRL42488.1"
                     /translation="MEKSINTVSIVGPTASGKTKLSVELAKHFNGEIVSADSMQIYTG
                     MQIATAKPTKEEMDGIPHHMMDFLPPDKTYSVASYVTDASKCIADIHSRGKLPFIVGG
                     TGLYVDSLLNNVSFSDDKRDEDYCLELRKIAEEQGTDRLLEMLAKFDPESAERLRESR
                     NLKRIIRAIEFYKTTGKTITQQIEESHKIPSPYITVKIGLNCRDRQVLYDRINKRVDI
                     MLEEGLLEEAERVINSDLSYTSIKAIGYKELIPYFKENKNLNDCVEKLKMETRRYAKR
                     QITWFKRDSEINWIYIDEYNSFEEIYSYAKAVIERGLLYG"
     gene            complement(23955..>24821)
                     /locus_tag="PLS64_01240"
     CDS             complement(23955..>24821)
                     /locus_tag="PLS64_01240"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42489.1"
                     /translation="EAAIRSVTKSIDDIKVADISNPFDIFSKQAINKAKEEEKSKSTF
                     KPEIVQPTSLVEDEKTDTESITDKSPIVSPKEVNVIVSEESIEKSKEPVLVNDNQTSI
                     KYIGEAFDTYIIAEKNNKDILLIDKHAAHERIIYEKLKAEKAADYRQILLAPVTVPLG
                     KTEYDAAINNLDMFSDCGFEVEDFGNSTVIVRSSPQYIPAVEIEDCITEMSGYISQGK
                     TDIFTEKMDWFYSNVACRSAIKAGNKSTPEELIGIVKHLEEHPEIKYCPHGRPICIVL
                     TKGEIEKQFGRV"
BASE COUNT         7152 a         5467 c         4233 g         7969 t
ORIGIN      
        1 tatcctaaac acaatactca actccgaatc tatgcgctaa tctacacaac accgaacaca
       61 attaaacggt tcggtgttgc acaataatta aaattcaatt atcagatatc gttctttact
      121 atatcctcgt agctttcacg cttaacaaca actcttgcct tgccgtcttt aaccattacg
      181 attgcaggac gagggattct gttgtaattt gacgccatag aatagttata agcacctgtt
      241 gaaagaacag caagtgtatc gcctacttca accttagcaa cctttgtatg ctcctgaacg
      301 aggtcgccgc tctcgcaaca cttacctgct accgttacga tttcatcagg ctctttgtca
      361 gccttacctg catttactac ggtataagca gactgataaa gtgcatatct gatgttatct
      421 gtcataccac cgtcaacgga tacatatgtt cttacattag gaatttcctt tttgccgcca
      481 actttgtaaa gtgtaatacc tgcttcgccg acaattgaac gacccggctc aataaagata
      541 tacggaacag gaagtccgtg ttctttagcc tttgccttaa cagcctctga aaccttttcc
      601 atatagtttt cgtaaggaac aggttcatca tccttagtgt acataatacc aaaaccgccg
      661 ccgaggttaa gctctgaaat aacgattcca agctcatcgt gaatcttgcc cataaagtca
      721 agcataattt cagcagcagt gacaaacgga tcaatatcaa aaatctgtga accgatgtgg
      781 caatgaagtt ccgtaagatt tacattatcg tacatctgtg cctttttaac cgcctcaaaa
      841 gcttcgcctg tttcaagagc aaaaccaaat tttgaatcaa tctgacctgt tctgatgaag
      901 ttatgagtat gtgcgtctac accgggctta attctaaaag aaatattagc cttcttaccc
      961 atttcaccgc aaatttcatt gagaagctca agttcgtaaa ggttatcaac aacgaacttt
     1021 cctacattag attcaagtgc aaatctgatt tcatcagcag ttttattgtt tccgtgaaag
     1081 tgaacttttt ccatcggaaa gcctgcctgt acggctgtat aaatctcacc gcctgatact
     1141 acatcaagac cgagtccctc ttcctttgca attctgcaaa gttccttaca agaaagagcc
     1201 ttacttgcat acaacggcat accattaccg tggtaatact tttcaaagga ctttacataa
     1261 gccttgcaca catttctgat tgttgtttcg tcaagaacat ataagggtgt accaaattct
     1321 ttggcaagtt cgagtgtatc gcaaccgccg attgtgagat gtcccttatt gttcacatta
     1381 agacattcgc taacgaacat tttactcata cctccgtatt tttaaaattt gctattgaag
     1441 tttcaataag tttccttatt tcatcatttc cgaagcctgt ttgtgcagaa aacggtatca
     1501 gcggaacatg ttcaattgta ctgaaaatgt ccttataata ctcaatctgc ttttcaaatg
     1561 cagtcttttt gagtttatca ctctttgtaa gaactgcggc aaagttaaat ccgcctctgt
     1621 aaaggaaatc tatcatatgc aagtcatcat cagtcggagg atgacgcaag tcaagaatct
     1681 gaataacgag cggacgctgt ctgtcctgtg caaaatagcc ttccacaagc tttgcccatc
     1741 tgtctttttc tgcttttgaa acctttgcat aaccgtagcc cggcaaatca accatacgaa
     1801 actctttgag cttataaaaa ttaatggtag ccgttttgcc cggctgagcg cttactcttg
     1861 caagagcttt tctctgaaca agtttattga taagagagga ttttcctaca tttgaatgac
     1921 ctgaaaatac aatttcaggc atatccgatt ccggcaactg atcaacacga cctgccgcaa
     1981 attcaaaagc gacttcatta aagttcataa aatccctctc tttcactgtt ttactgcgtt
     2041 tacagttttc gttgcagaac gaactgctgt catatcaatt ccgttccatt ggttatcggt
     2101 aattctcacg gtattattca ccgcaagtgc aactacctcc gaaaaattcc taacaggaat
     2161 aaacttaacc ttttcaagaa ccgccttatc gacatcttca agatcaggtt cgttatcctt
     2221 tggaatgatt actgttgtaa ttccgttttt aaaagcagcc atgcttttct ctttaagacc
     2281 gcctatagga agaacattcc ctctgagggt aatttcacct gtcatagcta tatcgtgtct
     2341 tgcaggcttt aaagtcagcg ctgagtaaat tgcggttgcc attgtaatac ctgcagacgg
     2401 tccgtccttt ggaacagcac cctcgggagc atgaatatga atatccttgt ttttgtaaaa
     2461 atcggaatca attccaagaa ccgccgcatg acttctaata caggtgattg ctgttttagc
     2521 agactcctgc ataacatcac cgagagaacc tgtaagttca atctttcctg taccctccat
     2581 aacggcaact tcaataggta agatttctcc gcctacagag gtccatgcaa gtccgtttac
     2641 tattccgatt tcatccattt tggcaagtga atcaggaata aactttttat ggcctaaaaa
     2701 tctttctacc tctttttcgt caatcttgaa tacttctgta ccctctgtaa gcttttcaac
     2761 ggcacatttt ctcataactg aggcaataag cctttcaaga gttcttacgc cggcttccct
     2821 cgtatagaag tctatgagag cgtatatggc tttctgagtg aatttgagtt cctttgaagt
     2881 aaagccgcac tcatcaagct gtttaggaat gaggtgcttc tttgcaatat ggaatttctc
     2941 ttctcttgta taactgctga tttctataat ttccattctg tcacggagcg gtgccggaat
     3001 agctgaaatg tcatttgcag ttgtaataaa catcacattt gacaaatcaa acggaagttc
     3061 aatgtagtga tcaacaaact tattattctg ttcgatatca agaacctcaa gcaatgccga
     3121 tgttggatcg cccttataat cagcggcaag cttgtctatt tcgtcaagaa taagtacagg
     3181 attattagtg ccggcatgtt gcaatgcatt aataattcta cccggcatcg aaccgatata
     3241 agtctttctg tggcctctga tttcagcctc atcacgaacg ccgccaagag cgattctttc
     3301 actttttctg cctattgcct ttgcaatcga ctgagcaatt gaggttttac cgacacccgg
     3361 aggtcctgca agacagatga tctgtccttt ttgcttttca cttaacacac gaacggcaag
     3421 ctgttcaatg attctctttt taactttgtc aagtccatag tgagtcttat caagagcctt
     3481 ctgaacttta gatataacga ttttatcttt agatgactta ttccacggca aatcaagaac
     3541 cgtatcaaga tatgtacgaa tcacagccgc ttcctgactg ccataaggca ttttgctgag
     3601 tttcttacat tctttataaa gaatgtcggc agacttttcg tcaagattaa gcttctcaat
     3661 tttatcgaca tagttttcag catcgtcaat cggtgaatca tcttcaccga gctcttcctc
     3721 gattgctctt ttctgttcac gaagaaaata atcacgctga ttttcatcaa ttctcatttt
     3781 agctttttga gatatttcgt cctcaagttt aagaacaaaa ttttcattta caagtacatc
     3841 aagaacagat tcaagtcttt cggattctgg gaaagtttca agaatcgact gtttaactct
     3901 gtagtcgaga atgagattag ctgtgataaa gtcggcaagt tcacccggtc ttttgcaaag
     3961 ggcaacctta aaaataatat ccgacggcat cttcggagaa atctcaagat acttgtcaaa
     4021 ttcatttttt acggttcgca taagagcgga atctcttgct gtaagatact cgctttcttc
     4081 ctcatacggt ttaacttctg caacaagaca cttttcgtca aaaacaggat ttataattgt
     4141 agccctgcat tgaccttcaa ttacaattcg tgtagtattt tcaggctgtt tcaaaacctg
     4201 aacaaccttg gcaattacac ccgtgtcata aacatcaaat atatccggtt cgtttattgc
     4261 ggcatccttc tgagaagtaa gaaaaacaag ttgatcggct ttcatagctt tatttatagc
     4321 cgtaatactc tttttccgtc caacatcaaa atgaataagt gattttggaa aaaccaccaa
     4381 ccctcgcaac ggaagaacag ggagagtcag atttttttgc atatcaaaat ccatttaaat
     4441 tcctttcata aaagcaaaaa gctgtattgt aattgcaata cagctttaat tcacaatatc
     4501 aagacgctgt gctgcgcttc tgacgcttct ttgcatcttt ttgagtcaat tttacggaat
     4561 ttgtctgtaa caattccttt ttgcgagtaa tttgcggagc agcaccgttt ttgataacat
     4621 cctctgtaat aataatctta tcaatagtag gatcagacgg agcctcaaac ataaggtctg
     4681 taagaatatc ttccataatt cctcgaagtc ctcttgcacc tgtatgctga tcctgcgcct
     4741 gttgtgcaat agctctgagt gcatcttctt caaaaagaag ttcaacaccg tcaagttcaa
     4801 aaagtttctt gtactgctga acaatagagt tttttggaac agtaagaatc ttgacaagtg
     4861 catccgtgtc aaggccgctc aaagcagtaa taacaggcaa acgaccgatg agttcaggaa
     4921 taattccgta ctttacaaga tcatgtgctg tgacctcttt catccaatcg gtcgaatcaa
     4981 gctcctgctt tgactgaaca agtgactcaa aacctataac agaagaaccc ttacgctttt
     5041 caactatctt ttcaagaccg tcaaatgcac cgccgcagat aaaaagaata ttcttagtat
     5101 caatctgtaa atattcctgt tgcggatgtt ttctgccacc ctgtggcgga acattggata
     5161 cagttccctc aacaatttta agcaaagcct gctgaactcc ctcaccgctt acatctcgtg
     5221 taatagaggg gttttcggat tttctcgcaa ttttatcaat ttcatcaatg taaattatgc
     5281 cgtgctctgc cttttcaata tcaaaatcgg ctgcctgaat gagttttaag agaatgttct
     5341 caacatcctc accgacataa cctgcttcgg tcagcgtagt tgcatcggca attgcaaaag
     5401 gaacatcaag agcctttgca agcgtctgag caagtaatgt cttaccgaca cctgtcgggc
     5461 cgagaagcag cacattactc ttagcgaaat ctacgctctc atcatttgaa aaagcacgct
     5521 tatagtgatt gtaaacagca acgctcaggg taacttttgc atgatcctgt ccgataacat
     5581 attcatcaag aattgccttg atttcctttg gttttaaaag ttcatcaacg gacagttcgg
     5641 gggtctgagg ttctcccgat tttgaaagtt tattttcatt ttcacgttca agctcgcctg
     5701 attcgcgaag aatattcatg cagagctcaa cacaccggtc acaaatatag acaccgttgc
     5761 cggcaataag tctgcctgcc atctcctgag gttttccgca gaaagagcaa taaatatctt
     5821 cgttattttt gttagccatc agtttacctc acctttatct tttttcgata accttatcaa
     5881 tcaaaccata ctcaagtgcc tgctgtgcag tcatgaagtt atcacgattg gtatcctttg
     5941 caataacatc aatcggctga cctgtgttgt cagcaagaat ctgattgagt cgttttttca
     6001 tatcaagaat gtgtcttgtg tgaatttcca tgtcagtagc ctgaccctga gcaccgcctg
     6061 aaggctggtg aatcataatg tcactgttag gaagtgcaag acgcttaccc ttagcgcctg
     6121 cggcaagaag gaatgcaccc atacttgcag ccatacccat acagattgtg gaaacatcac
     6181 acttgatgta attcattgta tcaaaaatag caaagccgtc tgttacgctg ccgccggggc
     6241 tgttgatgta aagactgata tcctttgtcg gatctttact ttcaaggtaa agcaactgag
     6301 caactacaac gcttgcactt gcactgttta cctcttcgtt aagcataaca attctgtctt
     6361 caagaagacg ggaatagata tcaaaagagc gttcgcccct gcttgactgt tcgataacat
     6421 atggtactaa tgccatttaa tcataacctt tcttaaatag tgattaacag atataagaaa
     6481 aatattcaaa ttacttaata acagctttgt cctttacgag attaagagcc ttttcaacct
     6541 taacatcttc tgtgagtgat tcagcaggaa cagcctcctt aaccttatca acatccatct
     6601 tgtaagcctc tgccatcttg ccgtattcag cgtcaaggtc agcctctgta acttcaatat
     6661 tttcttttct tgcaactgct tcaagagcaa gtcttaactt aactctcttt tcagcctctt
     6721 ctttatacat acccttgagt gcgtttgcat ccatacccat gtactgcata tatgtcttca
     6781 tatcaagacc ctgtgaacgg agacgcatat caaattctct taccatctcg ttagcctgat
     6841 tatcatacat tgcctcagga atttcaccct caaggagttc cataagcttt tcggaaatct
     6901 gattgtcaac atccttgtca gcctcatcct gaaggcgttt agcaacagtt tctttaagct
     6961 cagccttgta ctcgtcaagt gtttccttat cggaaacatc ctttacaaac tcatcatcga
     7021 cttccggaag ttccttagcc ttaatttcgt gaaggttgat cttgaattca gcttccttac
     7081 ctttaagctc ttcagcctga taatcatccg ggaacttaac tgtgattgta aactcttcac
     7141 ctgtcttgtg acctacaatc tgctcctcaa agccggggat gaagttgcct gatccaagct
     7201 taaggttgta gttttcagcc ttaccgcctt caaacgcttc accgtcaaca aaaccctcaa
     7261 agtcaataac tgctacatca tcattctgag cagctctgtc gtcaacagtt accattcttg
     7321 agttgcggtc acgaaccttc tcaatttctt cgtcaacgag ctcgtctgta acttctgttg
     7381 acttctttgt aacttcaatg ccgaggtagt tattaatttc catttcaggc tcaacaacaa
     7441 cattagcctt gaatgagaaa ccttccttgc ttgcctcaat agcctcaaga gtttcaaccg
     7501 aaactatctt gatacctgct tctttagcag cctcataaag agcgtccgga taacaatcct
     7561 gcatagcatc ttcatagaat acttccgagc cgtacatttt ctcaattaca tgacggggag
     7621 ctttgccctt tctgaaaccg gggatattga tacttttaac ctgcttttta taaactctgt
     7681 taacagcgtt accaaaagtt tcaccgtcaa cattaacctc taattcatat gaattagcct
     7741 gttctttttt tgtacattcc tttaatgcca ttttaatatt cctccaaagt ttaaattcaa
     7801 aatcaagaaa tcatcttatt atatcacaac aaaataattt tgccaataga aaatacagaa
     7861 tttatctgac taaaattggc ataaatgaca acctgtcaca agaaatcagc ttaaaatcaa
     7921 taccttcgta ttcaccttca atcgcaaacc tcatattctt ttttccgtga aggtgtccgt
     7981 aataacattt tttgatgtca tatttcaaaa gaacattaaa tatttcgtca cattttgttg
     8041 ttccgtaaac agggggataa tgtaaaaaca ctattggttc ctttcctgtt tttatagctg
     8101 attgtattga catttcaagt ctgccgactt ccctgttcag gacttttata tcattttgag
     8161 attcattctc aagaaaccat cctcttgtac cgcaaattgc gtaatcgtca acaacatatg
     8221 aattattgaa aaggatagat atcgaatcaa gtgaattatt tttcagaaac acatccattt
     8281 ttgtcttggt gctccaccaa taatcgtgat tgcctttatt gaaaattttc ttaccgggaa
     8341 gattttctat gaaagtaaaa tccgtaagag tttcttcaag cttcattgcc caggaaatat
     8401 cgcccgacac aacaaccgtg tcattatcgg taacaagctt tctccaatta ttttcaagtc
     8461 ttgaagtata atcgttccag cccgcgaaaa catccatagg cttatcttca ccaagtgaaa
     8521 gatgtaaatc cgctatagca aataatgcca ttaatctata ccgagtgctc cgagaacata
     8581 attctccata tcattaactt ctgtaacatc agagaatcga gcagttttat ttttaagatt
     8641 tgcaagaggt gcaggcactt cggcaccgga aaccttatga agttcatcaa ccatatcaaa
     8701 ttctgtttca ggaagtgttg cgtttggttc aacagcctca agaacagcct tagagaattt
     8761 atacggactt gcagttgacg caattacaga cggtgtcttg tcgcctgttt cctcaacata
     8821 ctgcttgtac acattgattg caacagctgt gtgagtatca caaaggtact tatcctgctc
     8881 aaacatctcg tgaattgtag cctctgtgtt cttatcatca cagaatccgc cgtagaactt
     8941 ttcgttgata acagccttta catcatcact tacttcatac ttgccttctg atttgagtgc
     9001 attcatccag cctcttgtaa cttcgtcatt atcacctgaa agcttgtaga gaagtctttc
     9061 aaggttactt gaaacaagga tatccattga aggtgaaatt gttgtataga agtttctgtt
     9121 cttatcataa acgcctgttt tgataaaatc tgtaagaaca ttattagagt ttgacgcaca
     9181 aatgaacttg ttgattggaa gtcccatgag acttgcatag tatgatgcaa ggatgttacc
     9241 gaagttacct gtcggaacaa caacattaat cttgtcgcca agctcgattt taccgtcatt
     9301 tacaagatcg cagtaagccg aaatatagta aacaatctgc ggaagaagtc tgccccagtt
     9361 gattgagttt gctgatgaga aaagcatatt attctcagcg agcttttctg caaccgaagg
     9421 tgtagtgaat atcttcttaa cacctgtctg agcgtcatca aagttaccct tgattgcaca
     9481 aacacgaaca ttttcacctt cctgagtatt catctgatgc ttctgcatcg gacttacacc
     9541 gtcaacagga tagaaaacaa caatctttgt gtgttcaaca tctttaaagc cttcaagagc
     9601 agccttgcct gtatcgcctg atgtagcaac gagaataaca gctgtcttgc cgtcatattt
     9661 cttcttgagt gactttgtga gaagatgcgg gagaatctgg agtgccatat ccttaaacgc
     9721 acatgtagga ccatgccaga gttcaagaat attcagggtt gttccgtcaa gattacagta
     9781 tgaaatagga gcaggatttt caccgccgaa tttctgagct gtataagcct tttctacgca
     9841 atcgctgatt tcctcttctg tgaaatcaga aagaaaatca gaaaatactt tctttgctct
     9901 tcctttgtaa tctgttttca aaagtgcctt gaaggtattt tcatcatact taggaaattc
     9961 ctgcggtaca aaaagaccgc cgtcctttga aataccctgt gcaatggcct gtgccgcaga
    10021 aactacttct ttggcatttt gtgtgctgtt gtataacatt gaaatcttcc tttcaaattg
    10081 ctttaaacat caagtatttt actacattat tcgctgattg tcaaagtttt caaagaaatt
    10141 atacgcattt tcgtaaaaaa tctttttcaa aacgctttcg ttgtagttat ttctaagaaa
    10201 aagttcataa atttcgccga ttgaatcgct tccgacaata tccctcggca aatcgcagcc
    10261 gtcaaaatcc gtaccgaagc aaagagaatt ttcgcatccg agcgacagca tatattcgca
    10321 atgcttcaaa acatcgttca tgcatgcttt atccggatta ttattaagaa aagcattgtg
    10381 caaatttaga cctataagtc cgcctctctg tattataatt ttaatctgct catctgtaag
    10441 atttctcgga ttttgagtaa ttgtccgtga atctgaatga gttgcgataa acggcttatt
    10501 cgtctgattg acaacatcat aaaacagttc atcgcttgca tgggatatat caattacaat
    10561 accgtacttt tccatttcgg ctactgcaag ttttccgaag tccgttaaac cgcatttatc
    10621 ttccgataaa acacctgaac caatctcgtt catctcattc catgtgagag tcattattct
    10681 gacgccgagt tctgcaaatc gctttacatt ctcgattttg ccgttcaatg cttttccgtt
    10741 ttctactgta aaaaacgctg agtttctata ctttgaaaaa gcattatctg tgaattcacc
    10801 gatacccaac aattcaattc caagtcggtt acattcggat ttcagataat cagccgattc
    10861 aaagaacaat ttttccgcct cattactgtc aagcgtgtcg ggtaaccata tagcatagca
    10921 ctgtaacctg tgggatttgg aattgttgtt taatttcact tcatatgagg gattatccag
    10981 ttcggacttt tcggttactg ccttatagag cgtgtcacaa tgcaagtcaa aaaaattcat
    11041 ggcaacctcc cgtcagaatg catcttcaat ataattaatg ccgacaagct taagattatc
    11101 actgttgaca aaaacttcgc atacatcaaa tcgacattct ttatctatat tgttttcata
    11161 catatacata tcggcagttt ttaaaatttt catctgcttt ttacggttaa ccgcatcaac
    11221 gggttgtgta agagaattct cttttcttgt tttaacctca acaaaggcaa ttacttcctt
    11281 attctcggct atgatatcta tttcgccgta aggcttttta taatttctgg caataatttt
    11341 atacttatgc tttttcagat attttgcggc ataagcctct cccttatcac ctgtttgttt
    11401 agtagtaaaa atcatttctt attatatatt ttcttcaaga agctcggtct gtgatactta
    11461 cacggtccgt atttaagaat cgcctcaata tgagcctttg tgccgtaacc cttatgcttt
    11521 gcaaataggt actgaggaaa ttctttgtcg tattcataaa gaagtctatc ccttgatacc
    11581 ttggcaagaa ttgacgcaca ggcaattgac attgactttg catcgccttt tacaatacat
    11641 tcgccttcaa ttgacaacgg aggcattctg ttgccgtcaa tcatagcata atcagccctt
    11701 ggttcaagtc cttcaactgc ccgtttcatt gcaagcatag cagcattaag aatattcatt
    11761 ttctcaattt cttcaacgct tgcaaatgca atgctgtatg ccaaggcctg ttcctttatt
    11821 acatcaaaca aagcctctct ctttttttca gaaagctttt ttgaatcatt gactccttca
    11881 ataacggtat tttcgggaag tataactgcg gcggcacaca caggtcctgc aagcggtcct
    11941 ctgccggctt catcaacacc gcagatattc agaaaacctc ttgcaatatt tttattttca
    12001 tattcaagcc aatccattgt tatctcccct gcggcatttc aactgtaatt ctgccaagct
    12061 ttgcacttct gaattcatca agcaacataa ttgcggctct ctcggtatct atctcaccgc
    12121 ctgaaacaag catacctctc tttttgccaa tcattttcaa aagctcgtat gaatcaatat
    12181 tttcgatatc ttcgttttct aatttaaatc ttgttataaa atcagcaggc ttgagttcct
    12241 tgatgaaatc aagaagtctc accgccaaaa gttcgatatc aagaatttgg tctttaaccg
    12301 cacctgtgaa cgcaagatgc tctccgactg tcttatcgtc aaactttggc caaagcacac
    12361 cggagtgtca agcatttcaa ggttctttgc aattgtatac cattgattac ctcttgtaac
    12421 gcccggtctg tcctcaacct ttgcacggtt cttctttacc attttattga taaaagaaga
    12481 tttgcctaca ttcggaatac cgacaatcat aattctgatt gtgggattta ccattccttt
    12541 ttcttttaat cttgcaatct tatggctcat aaccgtattt acggcaggtg caaatttgtc
    12601 aagacctctg ccacttttac agtcaaccgg gatagcaaca agattctgct ttttaaaata
    12661 gtcaatccac attttcgtgg cagtttgatt agccatatcg catttattga gaagaataac
    12721 tctcggcttg ttctgaacaa gttttgcaag gtcggggtta cgactgctga caggaattct
    12781 tgcatcaata atttctgcga cagcatcaac aagtttaaga cttgactgaa tctgtctttt
    12841 tgttttggtc atatgaccgg ggaaccattg aatagtctgc atttcgctca tatatgtaca
    12901 tcctttaaaa ttttttctgt taatataaat acttgaatct gtcaagcggg aaaaatataa
    12961 attgtgcttt tccgattaca tcatcgacag acacaaggcc tatactctta tcacggctgt
    13021 caagagaaat aattctgtta tcacccatta caaagatata accttcaggg ataacatacg
    13081 gaatttgcaa tggatttgac ggctctcgtg ttaaagaaga aacatacggt tcatcaattt
    13141 ttataccgtc aacataaact gcatttttac cgtaatccac aacaagcgtc tgtccttcaa
    13201 cggctataac ccgttttata attctctttt caatctgatt ttcagcgttg atagctacaa
    13261 tatcgccgca ttttggctca tacataaaat ttgtaatgaa aattttatca tcgctgtgga
    13321 gcgtgtcaag catcgaattt cctacaacat tggcatctct gatgataaaa gtaaacgcga
    13381 cggatataat tgcaaacaca aaaattattg tccgtgtaaa atcaaacata taatccgtga
    13441 tttttttatc gttaatttta attctgattt tattattttt atgtttttga ggcatataac
    13501 cctccgtaaa aaataaagct tatttctatt ataaaacaga aataagcttt tcacaatatc
    13561 aaaatttaaa ttaacattaa atttttacaa tctttagcat cagtacagat agccgaagtg
    13621 gtcgaacgga taaactacaa actgtgcctt accgattaca tcatcaacat ctataagacc
    13681 gatttttgta ctgcggctgt ccattgaatc ctttctgtta tcacccataa caaaaatttt
    13741 gccctcagga ataacctccg gaatttcatt gtcacctata ttgttgccgt aggttgaacc
    13801 ctctatgtac ggttcatcaa gaacaacacc gtcaactatg atttggtcgt tgtcataatc
    13861 gaccttaaca gtctgtcccg caacagcgat aacacgctta ataatcggac tgttatactc
    13921 tgcaccgtga gaaataacaa caatatcgtt atttttggga gtgtagaata gatttgttac
    13981 gataaccttg tcgttagtgg caagtgtgtt attcattgat ctgccttcaa catcaacaag
    14041 gcggaacaaa aagataaggc aaaccacaac aaccgaaatt gcaaacagga tacatcttac
    14101 ccagtcataa aaaccggaca ttgcgccttc ctcttttgca acaagaaatt gtttttcgga
    14161 aattgacggt ttcttatcca catacatttc aggattgata tctgaagaat cgttcacacc
    14221 ctcaggctga agcacagcca tattagcctc attgacatta tttttgcttt tcttcaactt
    14281 tgtcacctgc tataaagaaa aatagtaaaa aaagggactt ttaaaagtcc cttttgattt
    14341 cgtgaattac ttacgaatct gctctttaac cttagcagcc ttaccaactc tgtcacggag
    14401 atagtagagc ttgcttctgc gaaccttacc gtaacgaaca acctgtacat ctttaacatt
    14461 gggtgaatgg agagggaata ctctttcaac accaacgccg taagctactc ttcttacagt
    14521 aaatgtttca gctacgccac tgcctctttt agcaataaca gtaccctcaa acatctggat
    14581 tctttctctt tcgccttcac gaatgtttac tgataccttt acagtatcac ctacgctgaa
    14641 ctcaggtgtt gtttccttca cgcttgctgc agcaatagtc tttaatgcgt ccatttttta
    14701 tcctccttat ttttccgaca ttcttactca aacgagcagc ggactgtcac atcaaaatga
    14761 cggttaaacc gttatttgaa cctcgtcgag agttacgacg gtttcaaatg ctttaatata
    14821 ataccacata cggaataata atgcaagtgt tttttcaatt tagtttaaaa aatcggaata
    14881 tatcccgtca agctccgatt aatgtgatga tatattctca agctttttaa gatatttttt
    14941 gaaataatca ggcaattcgg aagtgaattc aatatattca ttggtttttg gatgaataaa
    15001 accgattttt cttgcatgaa ggcattgtcc ttcaaaatca actttttcat tctttacgcc
    15061 atacacctta tcacccgaca cgggatgacc tatataagcc atatgcactc tgatttgatg
    15121 agtacgacct gtttcaagct tgcattttat atgtgtataa cctttatacc ttgttattac
    15181 cgaataatga gtaaccgcat ttttgctgtt cttttcgata acacacattt tctttctgtc
    15241 tttaggattt cttccgattg gagcgtcaac tgttccctca tcatctttca gattaccaaa
    15301 tacaattgac tcatactccc ttgtaaaaga atgctcctta atttgttctg caagagaacg
    15361 gtgagtaaaa tcattttttg caacaatcaa aagaccgctt gtatccttgt cgatgcggtg
    15421 aacaatgccg ggacgaagta cgccgttaat tcccgaaagg ctgtcaccgc aatgataaag
    15481 caaagcattt acaagagtac cgtcataatt tcctgcggca ggatgcacca ccattccctt
    15541 tggcttattc acaacaagca agcattcatc ttcataaact atatcaagag gaatattttc
    15601 cgctttagcc tcataaggcg tcggatcggg aacatttaca acaacaatat caccttgcga
    15661 aagcttgtac ttttttgaaa caacagagtc gtttacaagc acagctccgt tctcaataag
    15721 attaacagca gcagagcgtg ataaatcaat atttgcatcc gaaataaatt tatcaagtct
    15781 tacaccgctt gtttcacaat taagtttaaa ttcattcatt gcttttgttg accttttttt
    15841 cggatttcat aacattagag aaaaacagaa cataaactat aagaagtagt gttccgactg
    15901 taatgcagta gtcggcaaag ttacaaacag ggggaaagaa tgaaacgctg atgtaatcga
    15961 ctacaaaacc gtaaaaaatc ctgtcaatca aattacctat tccgccgccg attataagtg
    16021 ccgcagaaat atagaacatc tttcccgacg gtttcttctt gaacataata tatattatta
    16081 ttgcaataag aacagcagta accgctacga aaaaccaacg catatccgaa aacattccga
    16141 aagcaacgcc tctattctca acataagtaa aatcaagtaa tccgtcaatg gcagttaccg
    16201 aaccgacagg ctgaagatag gtaagcacaa aatattttat tatctgatca gtggcagcta
    16261 aaagcacacc gattataaga gcaataacag gcattttatc ctccataaac ataaatcggt
    16321 gtgttttatc acaccgattt attgtaatag cagttattat ttaagattct gaacacagcg
    16381 gaaacagatt gtaggatgct ctgcacactg acccaccgtc ttgctgatag cccagcaacg
    16441 ctcgcacttt tcaccctcag ctttttcagg tttgatttca agctcaccgc cgttatcaac
    16501 aatttcaact tcggaaacta tgaatgctgc acaaagctca ggctcagcct ttttaaggaa
    16561 ttcaagtgtt tcgccgcctg cacaaagtgt tacctttgct tcaagcgaac ccttaattgt
    16621 cttatccttt ataagggatt cgagtgactt tttaacatta tcacgaagtt cgtggattct
    16681 gtcccagaaa gccataaatt tatcgtcaac atcaatagcg atgttttcgg gaatctcatt
    16741 aaagataaca tgttctgcat tctcatttga ggaatgaggc atgtatttcc aaatttcatc
    16801 tgatgtatac gcaaggagcg gagcaatcat tcttgtcatt gcgtcaagga taacatagat
    16861 tacggtctga gcggctcttc ttgagaggct gtcagccttt tctgtataaa gtctgtcctt
    16921 gagaacatca agatagaagt tagacatatc aacaacacag aagttgtgaa tgctgtgata
    16981 tacaatatga aactcataat tatcgtatga agttctaacc tttgtgataa ggtcattaag
    17041 ctttgcaagt gcccatttat caatcggcaa caccttgtca aagtcaacca tatctgtatc
    17101 gggattaaag tcagaaatgt taccgagaat gtatcttgcg gtatttctga tttttctgta
    17161 tgactcggaa atctgcttga ggatatcctt ggaaatatga acatcagtct gatagtcggt
    17221 tgatgcaacc caaagtctga gtacatcagc accgtactgc ttaataacat cctgtgggct
    17281 gatgccgtta ccaagcgatt tactcatctt tcttgattca tcgtcaagaa tcataccgtg
    17341 agtaaggaca gccttgtaag gtgctctgcc tcttgtagca acggatgtaa ggagtgatga
    17401 ctggaaccaa cctctgtact ggtcgttacc ctcaaggtaa aggtcagcag gtgattcaag
    17461 gttatcacgc tgttcaagaa cagcggcatg tgatgaaccg ctatcgaacc aaacatccat
    17521 aatatctttt tctttatcaa attcagtaca accgcacttt ttacactttg taccggcagg
    17581 aagaatctct gcggctgtgt gattgtacca agcgtttgag ccttctttac cgaataagtc
    17641 tgcaacagca agcatagcat ccttatcaat aagagcctct ccgcaatcct tacagaagaa
    17701 aatcggaatc ggaacacccc attttctctg acgggaaata caccaatctt ttctttctct
    17761 taccattgat gtaattctgt ccttgcccca tgcaggaatc cacttaaccg tattgatagc
    17821 gtcaacagcc tcatccttga aatcgtcaac cgaacagaac cactggtctg ttgctctgaa
    17881 aaggatagga gttttacatc tccagcaatg cggatactga tggacaattt tctttaatgc
    17941 aaaaagtgag ccgttttctt caagataggc ggcaatcttc ttattagcct catcagttgt
    18001 aagacctgca aactgacctg cttcttctgt aagaacaccg ttagaatcaa caggacagat
    18061 aatcggaatc tcagggtaat tctgacatac attgtagtcg tcaacaccgt gtccgggagc
    18121 tgtgtgtaca caacctgtac cgctttcgag agttacatgc tcaccaacga taacaaggga
    18181 ctctctgtca aggaaaggat gtgctgtttt tatatactca agctcactgc cctttaccgt
    18241 tgctacaact tcaaagtctg ttatacctgc ctcatcaaga gcagacttat aaagctctga
    18301 cgccataaca aggtattcat cacctgtttt gattacagaa tattcaaatc tcggacctac
    18361 gcaaattgcg acatttgcag ggagtgtcca agttgtagtt gtccaaatta caaacttaac
    18421 ctttgaagga tcgattccca tatttgagaa aacgccctta tcgtcggtta cattaaactt
    18481 tacatagatt gaatggcaag gatcttcagc atactcaatc tcagcctctg caagagcagt
    18541 cttacactcc ggacaccaat aaacaggctt taagcccttg tagatatagc cctttgtagc
    18601 catttctgca aacactctga tctgttcagc ctcaaactca tggttaagag tgatgtacgg
    18661 cttatcccat tcaccgatta cgccaagtct tttaaactgc gtacgctgat cgttaatgta
    18721 gccttttaca aactcttcgc acattttacg aagttcaaca acagaaattt cagcagaatt
    18781 accgataccg gctttctttc ttgctttaag ttccgtagga agtccgtgtg tatcccaacc
    18841 cggaacatac ggagctttaa agcctgccat attcttatat cttacgataa aatccttaag
    18901 aatcttatta agtgcatgac caaggtgaat gtcaccgtta gcataaggag ggccgtcatg
    18961 aagaatgaac agaggctttc cctcatttac tttcataaga ttttcgtaaa ccttttcatc
    19021 ttcccaattt tgaagtgtaa caggctcgct tttcggtaga cctgctctca tagggaagtc
    19081 tgtctttgga agattaagtg ttgaattata atcctgggac attttatatt ctctccaata
    19141 tctaaaatct aaataaaaat cagtcggcag ttacatcata gttatcaccg aacttgagat
    19201 gactgaactt gctctgtctt ttaggatgag cagatacttc ttcagtttca gcaatcggct
    19261 cagctttcat ttccgaaggt actgcaatct taacatcatc ttcaacctga ggctgtgcag
    19321 gagctgtcac ttcctctgta ggatactttt cgtcaagttc agcttttgaa gcctcaacat
    19381 cggaggttga aggaagatca tcaattgact taatatgctt actgtaaagt gaaataagtt
    19441 catttctgag attagccgca tctgactgaa gctgtaaata atgctctttc tcggcagcag
    19501 tcatattgtt tgcgtctaca agaagtgcct gagcctttgt ttcagcgtca cgaacaatct
    19561 ttgcagcctt ttcctttgct tctcttatgc aggcatcagc aaccttctgt gaagcaagca
    19621 aagcgtttct tactgtttct tcatctgctc tgtactgttc aactcttgtt gcaagaacat
    19681 caatcttatg aacaaggtta gttttttctt tcttcaattg ttcaaaagaa acaatgactt
    19741 catcaataaa cgagtcaaca tcttcagctc tgtaaccacc gttaagagtg gcttttctga
    19801 agcttatatt tttaatttca tcaattgtaa gcatttatct gcactcctta tttataatgt
    19861 ataaccgaaa ttctcattcg accttttcct gtagttccaa gtataccttt taaaacgaat
    19921 ttgccttttc cgcgaattgt aaaaacatca ttttccttta ggttaaaact gacattttta
    19981 gtttcttcaa aattaacaca tacgctgtcc gacataatga cagtttttgt tttttctcga
    20041 gacagtttac atacagcggc aacaatatta tcaagtcgta aagaagaaac tataaatgtt
    20101 ttttcttcta ctcctcttcc ctgcggaagc tttgacaaat cagcatcatc aaccttaacg
    20161 ccgactctgc cgacttttga aatctgcgac ttgacataat cggacaattc cgcttttaca
    20221 aatacaacag cccgtccgtc ctcaacaaga atatccccga cagtttcacg ctctattccg
    20281 agcgacatca aagaaccgag aaaatcacga tgagtgagtc ggtcgcattt tctgaatgaa
    20341 aattcaatgc cgcatatcgg gaacatactc gtactcggtt cgtcaaaaaa caagccaagc
    20401 atttttctct cggaatcatc atacccgcca aagaaaaggt agttgtcgaa atatgctttt
    20461 ttaagttcgg aaacaagaag tgcttgtttc ctttcactca taaaaggcat aaaatacggt
    20521 ttctgtcttg tcagacaaag gtcaataccg ttattaagcc gagcaatcag ctgtttgtca
    20581 tcatccgaag taaacaccgt tgttctcaag ctcatcaagt aaatcgtcgc ctgtcaggtc
    20641 aacattgctc ggaataatga tatatgtatt tgtagcgaca cgcttgattt taccacggtt
    20701 tgcatacgca acaccgctga ggaaatcaag gattcttctt gccatatctt tatttgtatc
    20761 ttcaagatta agaactactg tgtgagcctt cataagctca tcggcaattg tgcgggtttc
    20821 ttcgccgaaa cgctcaggct tgaataaacc aacctgaaga ttggtggtag cattaatgtt
    20881 tacgaccttg tttccgccgc cgagatctct ttctttttta gtgtttctta caggttcttc
    20941 tttataatct tcttctgcac cttcgtcagc ataaccatat tcatcgtact catactcatc
    21001 atcgggggcg tcccacatgc gtttaaactt atctacaaaa ccagccatta tataatcctc
    21061 ctaaaattat gtataattcc ttgcaccgaa taacgccgat cctaccctga ccatatttgc
    21121 acctgcaagt atagcctcat aataatcggc agacataccc attgaaagaa tattcatatc
    21181 tatattatct aatttttttt gcgagatgtc aataaatata ttatgcattt tattgaaaaa
    21241 ttcagaaatt ttctgtgaag attcacaaat cggaggtatt gccataagac ctttaactga
    21301 aataccgtcc aattcggcaa tctgacaaag taattcttca agctgttcgg gcataacgcc
    21361 tgacttgttt tcttctctgc cgatatttac ctcaacaaga acatcacagt ttttattgat
    21421 ttttaaagcc tgtttagaaa tttccgatgc ggagttttaa taatctgtct gaccttattt
    21481 gtctgcaaat gaccgataaa ctgaagagaa cagttgtcaa ggttatactt atcatacttt
    21541 gacaaaagct cctgaacctt gttttcacct atgtggtcaa gtccaagtga aattgcgtgg
    21601 ttaataacct caggttcaac catttttgtt gcggcaagaa atgtaatatc ctcacgcttt
    21661 cttccggact tttcggcagc ctcggctatt cgctcatcaa taaatttgta attgtattca
    21721 acgtcagtta acaacttttc cgtcatacaa atcatctcct tcaagaatta cctgatcata
    21781 taactcaact gttgtgccgt caacaaggtc tttgctgtcc ggctccggat cgcaaataac
    21841 ataatcatcg tcggcataca gaatagatat ctgtttgaat cgcacttcac ttccgtgaag
    21901 cacatataca ccctgaacct taacctgttc ggtatgttta ttgtcattgt catcataggt
    21961 tgtctttgtt acatagtcat catgaatagc ctttttgctg attctcaagc cagtgtatga
    22021 accaagacct atttcaatcg gttcctgtct tgcctgagca aggtcggaat tcatatagtc
    22081 acactcaaga ataagaaggg cgtcgctgtc ttttgattcc tgataaatcc tcttgatagt
    22141 ggcgggaatg ctttcagacg atgcgtcaga gaacagtacc gtagcggaac cgtcccacaa
    22201 agaaagtctt gttgcctcat cagccgtaac cttacaggca acataccatt tcaatcccgt
    22261 tacaaccttg cctgcaatat tgccggacac ttttgtctgc tttacattat caaggtcaga
    22321 aagagtcaga cgatcaagat tattataatc aagcgcattt tcatatccgt cacaatgtga
    22381 gagaaaacaa cctgattttt cagtcgtaat attacctgtt gattttcctg ttttgccttc
    22441 aagagtggct atttcagcct ggagttctga aattctgtca ttaaagttag tggctttgcc
    22501 tgtgtaaatc tgtctttggt ttattgaata aagcagatta tttgtgacat tatcaatatt
    22561 tgccaaatcg ccgtcattga tactgcgaat ttgtgaaatg atattattat ttatattgtt
    22621 gttgatagta tcaataccta tgttgccggc cgtgcttgtt ttttgaagtg tctgcaaatc
    22681 ggaaagctgt tcctgtaatg cgctgatttt tgactgtgaa gctgcgtcat catcatttgc
    22741 atatgatttt gcaatttcac cgccggcttt tacctcgtca ccgtcatgca ctgtatatga
    22801 aagcacaccc gaagtattat tctgaatcat attttcatca cggataataa atccgtttga
    22861 ataaatttta tccgtgactg ttgtttgaac agcattttca gtcggataca ttttaaaatt
    22921 tgtacttatc agaagatagg cggcataaac aactgcaaga actgataacg caatcatcaa
    22981 aattcgtctg aaaagcaatt tatccataaa gtaagcctct ttcgattaca gcctttgcat
    23041 aactgtatat ttcttcaaat gaattatatt catcaatata aatccaatta atctccgaat
    23101 cccgcttaaa ccaagtaatt tgtcttttcg catatctgcg ggtttccatt ttcaattttt
    23161 caacacaatc attcagattt ttattttctt taaaataagg aatcaattcc ttgtatccta
    23221 tcgcctttat tgatgtatag ctcaggtcgg aattaataac ccgttcagcc tcctcaagca
    23281 aaccttcttc aagcataata tctacacgct tgtttattct gtcataaagc acctgtctgt
    23341 cacggcaatt aagaccgatt tttacagtta tatacggaga cggaatttta tgtgattctt
    23401 caatctgttg cgtaatagtt tttcctgttg ttttgtaaaa ttcaatggca cggattattc
    23461 tttttagatt tctgctttca cgaagtcttt cagccgattc aggatcgaat tttgcaagca
    23521 tttcaagcaa cctgtctgtt ccctgttctt cggcaatctt gcgaagttct aaacaataat
    23581 cttcatcccg tttatcatca ctgaaagaaa cattgttcag aagtgaatca acataaagac
    23641 ctgttccgcc gacaataaac ggtaatttgc ctcttgaatg aatatctgct atacattttg
    23701 aagcatcagt tacatatgac gcaaccgaat atgttttatc aggcggaaga aagtccatca
    23761 tatgatgagg aattccgtcc atctcttctt tggtaggttt ggctgttgca atctgcattc
    23821 ccgtataaat ctgcatggaa tcggcagata cgatttcacc gttaaaatgc tttgcaagtt
    23881 caaccgaaag ttttgttttg cctgatgcgg taggaccgac aattgaaacc gtattaatag
    23941 atttttccaa aaaatcacac cctgccaaat tgtttttcaa tttcaccctt tgtaagaaca
    24001 atgcaaatcg gtctgccgtg agggcaatac ttgatttcag gatgttcttc aaggtgtttt
    24061 acaatcccta taagctcctc aggcgtactt ttattgcctg cctttatagc tgaacggcaa
    24121 gcaacattgc tgtaaaacca atccattttt tcggtaaaga tatcggtttt accctgagaa
    24181 atgtagcccg acatctcggt aatgcaatct tcaatttcaa cagccggaat atactgcgga
    24241 gaacttctta caataaccgt actgttaccg aaatcttcaa cttcaaagcc gcaatcggaa
    24301 aacatatcaa gattattaat tgccgcatca tactcggttt ttccgagagg gactgtaaca
    24361 ggagcaagta aaatctgcct gtagtcagca gccttttcgg ctttgagttt ttcataaata
    24421 attctctcat gagcggcatg tttgtctatc aaaagaatat ctttattgtt tttttctgca
    24481 atgatatatg tatcgaaagc ctctccgata tatttgatgc tcgtctgatt atcgtttaca
    24541 agaacaggct cttttgattt ctcaatagat tcttccgaaa caattacatt aacctcttta
    24601 gggcttacaa tcggactttt atcggtaata ctttctgtat cagttttttc atcttcaaca
    24661 agcgaagttg gctgaacaat ttcaggcttg aaagttgatt ttgacttttc ttcctctttt
    24721 gccttattta tcgcctgttt gctgaaaata tcaaacggat tggatatatc cgcaactttt
    24781 atatcatcaa tggactttgt aacagaacga atagctgcct c
//
LOCUS       DAOMVE010000010        23376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_348_length_23376_cov_3.834227, whole genome shotgun sequence.
ACCESSION   DAOMVE010000010 DAOMVE010000000
VERSION     DAOMVE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 23376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23376
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_348_length_23376_cov_3.834227"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1257)
                     /locus_tag="PLS64_01245"
     CDS             complement(115..1257)
                     /locus_tag="PLS64_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HRL42490.1"
                     /translation="MRDLKEIRKDIDKIDNQLIELFKQRMDCARDVGIYKQANNIPVL
                     NEGRENEILDAVEEKGGEYGASARLLYSNIMELSRALQHNIVGSGKELKSVINNASTD
                     IPSSGIKVAYQGIKGANGHEATLRLFPNGEAVNYKSFADVFSAVDNGEVAFGVLPVEN
                     SSAGSVSAVYDLILKHRFYIVKALDLPIDYCLAGLKQSAFEDIEIVWSHPQSLSQCAQ
                     YIADHGFDSVPFSNTAIAARDVAKEKRLNVAAICSYKACEEYGLKVLDNHLQDNDENT
                     TRFIVISKKLYIPEDANRISLCFSLPHVTGSLYSLLCRFNSLGLNLTKIESRPRQGRQ
                     FEYLFYLDFSGNVRSENVIELVSQLSEEMPEFSFLGNYAEPEFPIK"
     gene            complement(1265..2335)
                     /gene="aroC"
                     /locus_tag="PLS64_01250"
     CDS             complement(1265..2335)
                     /gene="aroC"
                     /locus_tag="PLS64_01250"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRL42491.1"
                     /translation="MSSTYGDKIKISVFGESHGNGIGVVIDGLPAGFEVDFDRVLTQM
                     ARRAPGKDKTATPRKEKDLPKVLSGMLGNKLTGAPLCAVIENTNTKSGDYGNLLDCPR
                     PGHSDYTAFVKYNASNDIRGGGHFSGRLTAPIVFAGAVCRQILESKGVKIAAHISSIG
                     NVSDSSFCPTEIDDALIEKLNNSSFALIDETAEKPMRDAVEDARMAQDSIGGTIECCV
                     TGIDAGYGEPMFEGVEGVIAKAVFGVPAIKGIEFGKGFELSTMRGSQSNDPFEYKDGK
                     VVTTTNNCGGILGGITNGMPIIFRAAVKPTPSISQPQRTVNLQTKENAELVIHGRHDP
                     CIVPRAVPVIEAVTAVAIINLM"
     gene            complement(2316..3614)
                     /gene="aroA"
                     /locus_tag="PLS64_01255"
     CDS             complement(2316..3614)
                     /gene="aroA"
                     /locus_tag="PLS64_01255"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRL42492.1"
                     /translation="MALSTDKSSKPYVRYLPFVPNGEVNIPPSKSDVHRAIICAALTR
                     GKCTIAPVALSNDIKATIGVIEDMGCTASIKDGVLTVDGTNIFKTDNVTLDCGESGSA
                     LRFFIPVAAAGGINATFVGHGKLPERPIGIFTEALPKAGVKCETEGGLPLKISGRLES
                     GTFEIPGNVSSQFITGLLLALPLVEGDSDIVLTSPIESVGYINMTIHTMAQFGVEIET
                     TDNGWHIKGGQSYIPHDYTTDGDWSQAAFFMVAGALGGKVTVNGVNRNSAQGDRKIAE
                     LLARFGAKVTQTDTSVTVEKGELNAIDIDASQIPDLVPVLTVCAAFAKGTTRIYNAER
                     LRIKECDRLTATAKLINDLGGKVTELPDGLVIEGIDTLKGGFCEGFNDHRIVMSAGVC
                     AVRLNGEIESSYALSINKSYPDFYIDYNNIGGKANVLDLR"
     gene            complement(3651..4700)
                     /gene="aroB"
                     /locus_tag="PLS64_01260"
     CDS             complement(3651..4700)
                     /gene="aroB"
                     /locus_tag="PLS64_01260"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRL42493.1"
                     /translation="MKTIHVSTGSPYDIMIERGILDNCGEIIKKLSNAEKITIVTDTN
                     VAPLYKWRVLNSLSQQGYQTTAHVFQAGEQSKNLNTIADIYDTLADFKMTRKDLIVAL
                     GGGVTGDMAGFAAATYLRGIDFVQIPTSLLAQVDSSVGGKTGVDIAQGKNLVGAFYQP
                     KAVLIDPDTLSTLPDQFITDGMAEIIKYGCIKDSEFFEMLANGDALEHIDDVIETCVK
                     IKRDVVNRDEKEAGERMLLNFGHTLGHAIEKIYNYTGITHGMAVAVGMVMMTKASEKH
                     GLTEAGTAYKIEEVCATYGLPTTDDASLEKIAEAAQTDKKTSGSSINVVLLEKIGTSF
                     TKKVAISDLLDFITP"
     gene            complement(4719..5549)
                     /locus_tag="PLS64_01265"
     CDS             complement(4719..5549)
                     /locus_tag="PLS64_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HRL42494.1"
                     /translation="MNIAVVGLGIIGGSFCKAIKKYTDHRVIGINRTKSTAEKALKEG
                     AIDEIGTHESLENADLIILSMYPQAVVDYVSRYGENIKKGAVVTDVSGIKRAICPQMT
                     ELSEKFGFVFAGSHPMAGKETNGFDVSDADLYKNASYIIVPCKADNSVVNMLSDFAKS
                     IGFGTVKITTPEEHDRMIAFTSQLPHVLACSYVLSPCCPNHKGFSAGSYRDVSRVANI
                     NSTLWSELFLENKDPLISELDILIDNITKIKDAIKAEDTDALKALLEKGREVKQALGE
                     "
     gene            complement(5638..6654)
                     /gene="aroF"
                     /locus_tag="PLS64_01270"
     CDS             complement(5638..6654)
                     /gene="aroF"
                     /locus_tag="PLS64_01270"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRL42495.1"
                     /translation="MIIVLKPSATEEQITKFTNMLKESYDVKVNKWDGVHSTVLGLIG
                     DTTKIDIEYIDAQDIVENVKRVQEPYKKANRKFHPDNTVIKINDNVTIGDGSLHIMAG
                     PCSVESEEQIVQIAKDVKASGATLLRGGAFKPRTSPYAFQGLKAEGLDLLKTARRETG
                     LPIVTEIMRASHIDMFENVDIIQVGARNMQNFELLKELGKIDKPILLKRGLSATIEEW
                     LMSAEYIMAGGNDKVMLCERGIRTYETFTRNTLDISAIPIIKKLSHLPVIVDPSHASG
                     KSWLVEPLAMAAVAAGADGLIIEVHNDPPHALSDGAQSLTPKQFDGVAKKVFGLKKAV
                     DKLN"
     gene            complement(6765..7301)
                     /locus_tag="PLS64_01275"
     CDS             complement(6765..7301)
                     /locus_tag="PLS64_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRL42496.1"
                     /translation="MNNIVLCGFMGCGKSTVGKNIARKSGRKFLDMDSYIEEKAGMTV
                     SEIFVKYGEEKFRDMEHDACVELAGLEGLVIASGGGAFTFKRNVEAFKGKDKIVFLDV
                     PLGIIKSRLKNDTTRPLLQRPDRDRVMKDLYDSRYPIYHAAADIVVKGKNTPLKTAFA
                     IIDAVSGTDVAEKPHKNA"
     gene            complement(7320..8165)
                     /locus_tag="PLS64_01280"
     CDS             complement(7320..8165)
                     /locus_tag="PLS64_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRL42497.1"
                     /translation="METKKFAVIGHPIGHTMSPFIHTRLFELQGVKAEYTKLDIAPEN
                     LEYEFKNTLSKLDGFNITIPHKQAVIPFLDEIDAKAEMYGSVNTVSNKNGISKGYTTD
                     PDGFLKALDAAGIMLNGRIVIIGCGGVARTMAYEVVLKKQPLLFAVRKEDLKIAEALC
                     DEIRKTVAGCDVSFCLIDELSGDIDVLINATPIGMFPKIENQPVSDSVINRCANVFDA
                     VYNPLETMLVKKARANGATAESGMAMLVWQAVVSHEHWDGSVYDKGDIAQLCIDSAKE
                     LEKNF"
     gene            8412..8639
                     /gene="acpP"
                     /locus_tag="PLS64_01285"
     CDS             8412..8639
                     /gene="acpP"
                     /locus_tag="PLS64_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRL42498.1"
                     /translation="MVLEKVKAILAEQFDVEEDKITADTDLQEDLGADSLDVVDLLMS
                     IEDEFEVEVPDEEIENIKTVGSLVSYIEANS"
     gene            8913..10352
                     /gene="proS"
                     /locus_tag="PLS64_01290"
     CDS             8913..10352
                     /gene="proS"
                     /locus_tag="PLS64_01290"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRL42499.1"
                     /translation="MANNKKMVEAITSRDEDFAKWYTDIVKKAELVDYSGVKGCMVIR
                     PYGYAIWENIQADLDRRFKETGHENVYMPMFIPESLLQKEKDHVEGFAPECAWVTVGG
                     QEKLQERLCVRPTSETLFCEHYKKIINTYRDLPKLYNQWCSVVRWEKTTRPFLRTSEF
                     LWQEGHTMHETEEEAREETLRMLHVYSDFYKETLAIPAVIGRKTEKEKFAGAEETYTI
                     EPMMHNGVALQGGTSHYFGDGFAKSFGITYTGKDNKLHYPHQTSWGVSTRMIGALIMV
                     HSDDDGLVLPPKISPIQVALIPVAQHKEGVLEKAEELRKALAEKFRVKLDSSDHAPGW
                     KFAEYEMKGVPVRVEIGPKDIEKNQCVVVRRDTREKAFVSIDELVPFVENLMVTIHND
                     MYDRALKNTQEKTFTATTFDEFVDTAKNKPGFIKAMWCGDTACEEKIKEVTGGVKSRC
                     IPFNEEHLSDVCVCCGKPAKHMVFWGKQY"
     gene            10401..11333
                     /gene="metA"
                     /locus_tag="PLS64_01295"
     CDS             10401..11333
                     /gene="metA"
                     /locus_tag="PLS64_01295"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HRL42500.1"
                     /translation="MPIKVQSNLPAIKVLESENIFVMPNEVALRQDIRPLKILILNLM
                     PTKIETETQLLRLLGNSPLQVDIELLQMASHTSKNTSPHHLTTFYKTFNQVKNESFDG
                     MIITGAPVEQLDFEEVDYWGELCEIMEWSKHNVCSTFHICWGAQAGLYYHYGIKKHLL
                     PEKLSGIYTHKVLVPHHKLMRGFDDTFTIPHSRHTGIDEEALKRCRGLEVLAVSEIAG
                     SCVICSRNGRQVFVTGHAEYDRDTLAKEYFRDKNKGLNPNVPYNYFPNDDASKTPPMI
                     WRSNATLLYTNWLNYFVYQATPYDLRELINRYPH"
     gene            11455..12270
                     /gene="proB"
                     /locus_tag="PLS64_01300"
     CDS             11455..12270
                     /gene="proB"
                     /locus_tag="PLS64_01300"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRL42501.1"
                     /translation="MKICNETGVFMEKKRTVIKVGTSTLTYENGKINYRRVEQLCKVL
                     SDLQNRGEQVIFVSSGAIAVGMGKAGLDKRPTETKKKQALAAIGQCELMFMYDKLFGE
                     YNHSVAQLLLTRHAVETEQKRQNVINTIDELLRMNIIPVINENDTVTIDELEGNNFGD
                     NDMLSAIVAGLVAADRLIILTDIDGLYDSNPRTNPNAKKLDIIEKINAEIFEMAAGTG
                     SNRGTGGMITKLQAADYATKRGVEVSVINGSNPENLYEVIDGNKIGTKFLPVK"
     gene            12284..13528
                     /locus_tag="PLS64_01305"
     CDS             12284..13528
                     /locus_tag="PLS64_01305"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRL42502.1"
                     /translation="MTTLEQMGAKAKDASRFLMTAGSKKDIALGAIADALCENCEEIL
                     AANKIDLENGEKSGLTASLLDRLRLSEERINGMADGVRQVAALPDPVGRVLDGRTLKN
                     GLQIEKVTVPMGVIGIIFEARPNVTSDAAALCLKAGSAVILRGGKEAFHSNMAIAKVM
                     RDALEKAGFPRDCVALVEDTTRQSATELMQLSDYLDVLIPRGGAGLIKSVVENAKVPV
                     IETGVGNCHIYVDKSADIEMAKNIVFNGKTSRPSVCNALETLLVHKDIAEKALPVIKA
                     ELDKKNVEIRGCDRTKQILGDCVVPATEEDYRIEFLDYIIAVKVVDSLDDAIAHIQKY
                     STGHSECIVTSDYNSANEFTARIDSAAVYVNASTRFTDGGEFGLGAEIGISTQKLHAR
                     GPMGLNELTSSKYIIRGNGQIR"
     gene            complement(13529..13813)
                     /locus_tag="PLS64_01310"
     CDS             complement(13529..13813)
                     /locus_tag="PLS64_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42503.1"
                     /translation="MPSFEEEAFNRAQQMHRKPQFNREAPRREVPKQPEKQEEPKSEI
                     PEPPVPKKDDGLLNLMFKNKEQSLILLLVILLMEENTDPSVLLALMYLLM"
     gene            complement(13828..14181)
                     /locus_tag="PLS64_01315"
     CDS             complement(13828..14181)
                     /locus_tag="PLS64_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42504.1"
                     /translation="MSDLQDKLNQILQNPEAMKQVQSLGAQLGLTGEQKQPPKPKPPE
                     NNLMSVLGGDDTIGTIAKFAPLLSSLNNSDDTTRLLDSLRPFLSHERQEKLDKAEKML
                     KLFRLMPLLRENGLF"
     gene            complement(14183..14419)
                     /locus_tag="PLS64_01320"
     CDS             complement(14183..14419)
                     /locus_tag="PLS64_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42505.1"
                     /translation="MSDNKKVNKLLGEVSKKYGISKEQLESAAQSGNIENLLKNTNPN
                     QSKQIESVLSDPEKAKKLLQSPQAQALMKLLGGE"
     gene            14477..14947
                     /locus_tag="PLS64_01325"
     CDS             14477..14947
                     /locus_tag="PLS64_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRL42506.1"
                     /translation="MRILVVSDTHGDLRSLIKAVDAQRKAEIIVHCGDGEEQQRFLKD
                     NYKDKMIVAVRGNCDWNSFFPSKEIFKACGKTIFVTHGHLYDAKSGLYRITSAAREAG
                     ADILLFGHTHMPLTLYEDGLYIMNPGSCHGYYASYGIIDITDKGEIVTNTVALK"
     gene            15009..15872
                     /locus_tag="PLS64_01330"
     CDS             15009..15872
                     /locus_tag="PLS64_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42507.1"
                     /translation="MNFDGFDFDDNIKQTFNTLDIQGRVPHAVVIESRDSEKAMNLAV
                     FLSMYAVCTEPVKPCGHCEQCHKAKNKAHADIKYVELPNDRKQNSIEQMREIIGDSVI
                     KPNEADAKVYIFPFADTRLSDIVQNSFLKLLEEPPQNVYFIMLCENSKKLLPTILSRS
                     SVFRLKTKDVFSEDAVAGAKKIVKGILSTREYNLMQALYALSDKNLADEILLVVKLIL
                     RDGMAKSVGADAVFDEECAGELARRFTRAKLISMIELTENAKLKIPKHININLLTTWL
                     CGEYRRISWQR"
     gene            15860..16726
                     /locus_tag="PLS64_01335"
     CDS             15860..16726
                     /locus_tag="PLS64_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HRL42508.1"
                     /translation="MAEIIGVRFKEVGKVYYFDPLDNKLNTGDRVIVETARGLECGEV
                     ATPNKTVDDAEISHPLKPLIRIATEKDLNHLAENKLKEKEAYRICEQKIANHKLEMKL
                     VNVEYTFDNSKILFYFTADGRVDFRALVKDLASVFRTRIELRQIGVRDEAKMLGGLGI
                     CGKPFCCASFMGEFQPVSIKMAKEQGLSLSPVKISGTCGRLMCCLKYEQEAYTDLLKH
                     TPKVGAIVNTPEGRGLVVENNLIAGTLKVKLNNTPEDAAPKSFTVKQCKLVKDGYIKL
                     DKKEMEKFKGLE"
     gene            16871..17041
                     /locus_tag="PLS64_01340"
     CDS             16871..17041
                     /locus_tag="PLS64_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010075348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRL42509.1"
                     /translation="MAYTISDDCIMCGACADNCPCDAISEGDGKYEINADACVDCGSC
                     AEVCPVGAPQEG"
     gene            17118..17858
                     /locus_tag="PLS64_01345"
     CDS             17118..17858
                     /locus_tag="PLS64_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRL42510.1"
                     /translation="MNNKHLEPLGSGIKIYVSDSYRFSTDTILLADFSKQIGAKKCVD
                     LGTGCGTIPLLWYKNNNRLDITAVEIQENACKLFSQSLEYNNLQDKIRIVNSDLNKLD
                     GALPLGSFDLVACNPPYKISGGGITNPENAKLVARHESECTLDDVCRCGSSLLQFGGR
                     LCICQRPERLADAMEAMRKYSVEPKKLRLVQQRKSKAPKLFLLEGRRGGKRGFLEVMP
                     TLFIEDESGNFSEEMLNIYGSYKEDYIN"
     gene            17855..18679
                     /gene="rsmI"
                     /locus_tag="PLS64_01350"
     CDS             17855..18679
                     /gene="rsmI"
                     /locus_tag="PLS64_01350"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRL42511.1"
                     /translation="MSGILYVTGTPIGNLGDFSPRAAETLESVDFIAAEDTRVTLKLL
                     NHFGIKKPMVSYFEHNKRERGEIICNRIEQGENCAIVTDAGMPCISDPGEDLIKLCEE
                     RGIKTVVIPGPSAVISALAVSGLETGRFTFEGFLSVNKKSRNEHLDSLKNEHRTMIFY
                     EAPHKLPQTLRDLYNFFGDRKLSIVRELTKVHEEVIRTTTKYAAENYADGSLKGEFVL
                     VLEGKKQEEKSEEYTLEFAVETARKLIENGAKPTEAAKEAASITGFKKNEIYRELL"
     gene            18680..19990
                     /locus_tag="PLS64_01355"
     CDS             18680..19990
                     /locus_tag="PLS64_01355"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HRL42512.1"
                     /translation="MTYENAVEKIHSLLTFGSRPGLDRMRILLDRLGNPQDRLKFIHI
                     AGTNGKGSVCAMLSSALVAAGYKTGLFISPYITDFRERIQINNCMVSESVLTDAVEKT
                     FPIIEQLREEGTIITEFEYVNALQFYIHANADCDIVVLETGMGGLLDCTNTIKSPLCA
                     VITTIGLDHTAILGDTIEKIAQQKCGIFKPDSMAVTSKQDIRAMKVIESTAREKKIPL
                     ATSESVNIDVIETTLKGSRFMFNGVEIDLPLSGDHQLENAKTALATLDMLRCNSLISI
                     TDEQIANGFAKAVNPARLELLSEKPIVLLDGAHNPNGIEALKSAIQKFLPNKYIIAIT
                     GMLADKDVSTSVKLLDGVFDRVYTVPVHNPRAMHPAKLMQQYARFVDDTVTFESAEHA
                     FDMAFEQAEKTDCAVVICGSLYLAGEIRPYIINKIKAENESAEK"
     gene            20091..20393
                     /locus_tag="PLS64_01360"
     CDS             20091..20393
                     /locus_tag="PLS64_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356093.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HRL42513.1"
                     /translation="MLKTEFKNNILTAYIDGEIDHDSAAGIRTKIDAAVQTNKPKLLC
                     LDFSKVSFMDSSGIGLVMGRYRQMKLVGGALRVINIPDRLYRLFSMSGLESLGVLR"
     gene            20390..20830
                     /gene="spoIIAB"
                     /locus_tag="PLS64_01365"
     CDS             20390..20830
                     /gene="spoIIAB"
                     /locus_tag="PLS64_01365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HRL42514.1"
                     /translation="MKTVNEMKLCVPSKSCNEAFARCVVSAFVMSLDPTVNELSDLKT
                     AVSEAVTNCIVHGYRHQSGTIYINGKITSENKVIITIRDKGCGISDIKKAMEPLFTTA
                     PEEDRAGLGFAVMQSFCDKVRVRSKPDSGTTVTLEKIIGSENDR"
     gene            20820..21479
                     /locus_tag="PLS64_01370"
     CDS             20820..21479
                     /locus_tag="PLS64_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL42515.1"
                     /translation="MTDKNKLADKNMGLVHACCKRFSGKGIEYEELFASGCLGLAKAI
                     NNFDESRNLQFSTYAFPVIMGEIKRLFRDGGAIRVSRTLKELSLAISRLNNESKLKNG
                     RELTVSELAERLDTSEEKIIDALNSTRVPLSLTAEYDSEGNPQLDVPTDDIQDEISER
                     LSLEQAVKSLCETDRKIIQLRYYRSKTQTQTAKILSMTQVQISRREKKILTAIRDKMS
                     V"
     gene            complement(21594..22103)
                     /locus_tag="PLS64_01375"
     CDS             complement(21594..22103)
                     /locus_tag="PLS64_01375"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRL42516.1"
                     /translation="MKNIVLIGMPGSGKSTVGVLLAKALGYSFVDVDLIICKEAGKPL
                     QEILDNEGLDFFLHLEGKIGAELDYDKTVIATGGSMVLSENAMENLRKNGKVVFIDVD
                     LDEIKRRVTNIKTRGIAFGKGETLDDVYRVRYPLYKKYADITVSMELSSIETTVDAIV
                     EKLKLEKQN"
     gene            complement(22105..>23376)
                     /gene="pyk"
                     /locus_tag="PLS64_01380"
     CDS             complement(22105..>23376)
                     /gene="pyk"
                     /locus_tag="PLS64_01380"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRL42517.1"
                     /translation="GQNVAILLDTKGPEVRVGTFKDGSVELVEGAEFSLFKNKEEGDE
                     TGVSLSFPKLVDIFESEGQEAIGRELLLDDGIISLVVKSVNPESIVCTVNKGGVLKNR
                     KSINIPGYFINMPYVSAQDRKDIEFGLNHGATVVAASFVRNHDDVRTLRDFIDSLGYE
                     YVEIIAKIENQSGVDDMDAIIDYADGIMVARGDMGVEIPFIKLPEIQKTIIRKVVSRG
                     KYVITATQMLESMTTAPRPTRAEISDVANAVYDGTSAVMLSAESAAGKYPIESVKALD
                     AICSEAEENGEFTALHEYVEEHGMKDTNPIRSSICKAAKNIAQAVGAKAIIVESATGR
                     VARAMVHYRPDCPVIAVATSQLVCRKLCLNWGVMAVMGEEKRTSDSITKQAMEKALST
                     GVVKKGDTVVVLSSNKTCPTSSTDSLNVRIL"
BASE COUNT         6610 a         5176 c         5136 g         6454 t
ORIGIN      
        1 caaactgtct tcccgacatt aattttccat tttcaatttt caattttaca tttaattaaa
       61 actatctaca cgaattgggt gcagcgacac gctgccggcg gctcgccgtg cggtttattt
      121 aatcgggaac tcgggttctg cgtagttgcc caaaaagctg aattcgggca tctcttcgct
      181 aagctgactt acaagttcaa taacattttc acttctgaca ttgcccgaaa agtcgaggta
      241 gaacagatat tcaaactgtc tgccctgacg gggacgggat tcgatttttg tgaggttaag
      301 accgagcgaa ttgaatctgc acaaaagact gtacagcgat cccgtaacat gaggaagcga
      361 gaaacaaaga ctgattctgt tcgcatcttc gggaatgtac agctttttgg agatgacaat
      421 aaaccttgtt gtgttctcgt cattgtcctg aagatgattg tcaagaacct tcaagccgta
      481 ttcctcacac gctttgtatg aacagattgc cgcaacattg aggcgctttt cctttgcaac
      541 atcacgggcg gcaattgccg tgttggagaa cggaacagaa tcaaagccgt ggtcggcaat
      601 atactgcgca cattgtgaaa gtgactgcgg atgcgaccac acgatttcaa tgtcctcaaa
      661 agcagattgt ttaagacctg caaggcagta gtcaatcggc aaatcaagtg cctttacaat
      721 gtaaaaacgg tgcttgagaa taaggtcata aactgccgaa acagagcctg cgcttgagtt
      781 ttcaacagga agaacaccga atgcaacctc gccgttgtca acggcagaaa atacatctgc
      841 aaagcttttg tagttgacag cctcgccgtt cgggaaaagt cttaaggttg cctcatgacc
      901 gttagcaccc ttgattccct gatacgcaac cttgattccg cttgacggaa tatccgtgct
      961 tgcattgttg ataacgcttt taagctcttt gccgctgccg acaatgttgt gctggagcgc
     1021 ccttgaaagc tccataatgt ttgagtagag aagtcttgcg gatgcgccgt attcgcctcc
     1081 cttttcttca acggcatcaa gaatttcatt ctctctgccc tcgttaagaa cgggaatgtt
     1141 gtttgcctgc ttgtagatgc cgacatccct tgcacaatcc attctctgct taaaaagctc
     1201 aataagctga ttgtctattt tgtcaatatc ttttctgatt tcttttaaat ctctcataaa
     1261 gacctcacat aaggttaata attgcaacag ccgtaacagc ctcaataacg gggactgctc
     1321 tcggcacaat acacggatcg tgtctgccgt gaataacaag ctctgcattt tcttttgtct
     1381 gaagatttac agtcctttgc ggttgtgaaa ttgacggtgt aggctttaca gcggcacgga
     1441 aaataatcgg cattccgttt gtaattccgc ccaaaatacc accgcagttg tttgttgttg
     1501 taacgacctt gccgtctttg tattcaaacg gatcgttcga ctgcgaacct ctcattgttg
     1561 aaagctcaaa gcctttgccg aactcaatgc ccttaattgc gggaacaccg aaaacagcct
     1621 tggcaataac gccctcaaca ccttcaaaca tcggctcacc gtagcctgcg tcaatgcctg
     1681 ttacgcaaca ttcaattgtt ccgccgatgc tgtcctgtgc cattctggca tcttcaacgg
     1741 catctctcat aggtttttcg gcagtttcgt caatgagtgc aaacgatgag ttgttcagct
     1801 tttcaatgag tgcgtcatca atttccgtcg gacagaagga gctgtcggaa acattgccga
     1861 tactgctgat atgggcggca attttaacgc cctttgattc aagaatctga cggcagacag
     1921 cacctgcaaa aacaatcggt gctgtaagtc tgcccgaaaa atgaccgccg ccacgaatat
     1981 cgtttgacgc attatatttt acaaaggctg tgtagtcgct gtgaccgggg cggggacagt
     2041 cgagaagatt gccgtagtca ccgctttttg tgttggtgtt ttcaattacg gcacaaagcg
     2101 gagcgcctgt cagcttgttg ccgagcatac ccgaaagaac cttcgggagg tctttttctt
     2161 ttcgtggagt tgctgtttta tccttgcccg gagcacggcg tgccatttga gtgagaaccc
     2221 tgtcaaagtc aacctcaaat cctgccggaa gtccgtcaat tacaactccg attccgttgc
     2281 cgtggctttc gccgaaaacg gaaattttta tcttatcacc gtaggtcgag gacattcgcc
     2341 ttacctccaa tattattata atctatgtaa aaatcagggt aacttttgtt gattgaaagc
     2401 gcatagcttg actcaatttc accgttgagt cttactgcgc agacacctgc cgacattaca
     2461 attctgtggt cattaaagcc ctcgcagaat ccgcccttga gggtgtcaat tccctctatg
     2521 acaagtccgt caggcaactc agtcacctta ccgccgagat cgtttatcag cttagcggtt
     2581 gccgtaagac ggtcacattc ctttatgcga agtctttcgg cattgtaaat cctggttgtt
     2641 ccctttgcaa atgcggcaca aaccgtcagc acgggtacaa ggtcgggaat ctgcgaagca
     2701 tcaatgtcaa ttgcattaag ctctcccttt tcaacggtta cggaagtatc cgtctgcgtt
     2761 acctttgcac caaaccgtgc aagcaactcg gcaatcttcc tgtcaccctg tgcggaattt
     2821 ctgttgacgc cgttcacagt cacctttccg ccgagtgcgc ctgcaaccat aaagaaagcc
     2881 gcctgcgacc agtcgccgtc tgttgtgtaa tcatgcggaa tgtatgactg accgcccttg
     2941 atgtgccagc cgttgtcggt tgtttcaatt tcaacaccaa attgtgccat agtgtgaatt
     3001 gtcatattta tgtagccgac gctctcaatc ggagatgtca gcacaatgtc gctgtcaccc
     3061 tcaacaagcg gaagagcaag cagaagtccc gtaatgaact gcgaacttac attaccggga
     3121 atttcaaaag ttccgctttc aagccgaccg ctgattttga gcggcagacc gccctcggtt
     3181 tcacacttta ctcctgcttt tggcagagcc tccgtgaaaa ttccgatagg tctttcggga
     3241 agctttccgt gaccgacaaa ggtggcatta ataccgccgg ctgcggctac gggtatgaaa
     3301 aatcgcaaag cactgccgct ttcgccgcag tcgagcgtta cattatctgt tttaaaaata
     3361 tttgtaccgt caacagtcag cactccgtcc tttattgagg cggtgcatcc catatcctca
     3421 atcacgccga ttgtcgcctt gatgtcattt gacagagcaa cgggtgcaat tgtgcatttg
     3481 ccccttgtga gggcggcaca gattattgca cggtgaacat cgctctttga cgggggaatg
     3541 ttcacctcgc cgtttggtac aaacggaaga taccgtacat acggcttact gcttttgtcg
     3601 gttgacaaag ccatacttcc acagcctttc attaaaatat gtttaccgtg ttacggtgtg
     3661 atgaagtcga gcaaatcgga gattgcaact ttcttggtaa agcttgtgcc gattttctca
     3721 agcaaaacaa cattgattga cgagcccgaa gttttcttgt ctgtctgagc cgcctcggca
     3781 attttttcaa ggcttgcgtc atcggtagtc ggcaagccgt aggttgcaca aacttcttca
     3841 atcttgtatg cagttcctgc ctcggtaagt ccgtgctttt cgcttgcctt tgtcatcatt
     3901 accataccga cggcaactgc cataccgtgt gtgatgcctg tatagttgta gattttttcg
     3961 attgcgtgac cgagtgtgtg accgaagttt aaaagcattc tttcccctgc ttccttttcg
     4021 tcacggttta ctacatcacg cttaattttt acacaggttt caattacatc gtcaatgtgt
     4081 tcaagagcgt cgccgtttgc aagcatttca aaaaattcgc tgtccttgat acagccgtac
     4141 ttgataatct cagccatacc gtctgtgatg aactggtcgg gaagagttga aagagtgtcg
     4201 ggatcaatca aaacagcctt tggctgatag aatgcaccga caaggttctt gccctgtgca
     4261 atgtcaacgc ctgtttttcc tcctacggat gagtcaacct gtgcaagcag agatgtcgga
     4321 atctgaacaa agtcgattcc tctcagatat gttgcggcgg caaagcctgc catatcgccc
     4381 gtaacaccgc cgccgagtgc aacgataagg tcttttcttg tcatcttaaa atctgcaagt
     4441 gtgtcataga tatctgcaat tgtgttaaga ttctttgact gctcgccggc ctgaaaaaca
     4501 tgggcggtag tctgataacc ctgctgtgaa agagagttga gaactctcca tttgtacagc
     4561 ggagcaacat ttgtatctgt tacaatagtg attttttctg catttgaaag ctttttgata
     4621 atctcgccgc aattatcaag gatacctctt tcaatcataa tgtcataggg acttcccgtg
     4681 gaaacatgaa tagttttcat aaaagttctc cttcctcttt attctccgag tgcctgtttg
     4741 acctctctgc ccttttcaag aagagccttg agagcgtcgg tatcttcggc ttttatcgca
     4801 tctttgattt ttgtaatatt atctataagt atatcaagct cggaaatgag cggatctttg
     4861 ttttcaagga aaagctcact ccaaagcgtt gaatttatgt ttgcaactct cgaaacatct
     4921 cggtaacttc ctgccgaaaa gcctttgtgg ttcggacagc acggacttaa aacatatgaa
     4981 cacgcaagca catgcggaag ctgtgatgtg aaagcaatca ttctgtcgtg ttcttcggga
     5041 gttgtgattt ttactgtgcc aaagccgatt gattttgcaa agtcggacaa catatttaca
     5101 acgctgttat cagccttgca cggaacgata atgtagcttg cgtttttgta aaggtcggca
     5161 tccgaaacat caaaaccgtt tgtttccttg cctgccatcg gatgactgcc ggcaaaaaca
     5221 aagccgaatt tttcggaaag ctctgtcatc tgcggacaaa ttgccctctt tatgccggaa
     5281 acatcagtta caaccgcacc tttttttatg ttttcgccgt accttgaaac ataatcaaca
     5341 accgcctgcg gatacattga aaggataatt aggtctgcgt tttcaaggct ttcatgtgtg
     5401 ccgatttcat caatcgcacc ttcttttaaa gccttttcgg cagtagattt tgttctgttg
     5461 ataccgatta cacggtggtc ggtgtacttt tttatcgcct tgcaaaaact gccgccgata
     5521 attccgagtc ctacaactgc aatattcact gcattagcct ccgtttgtgt attctttagt
     5581 aaagttaatt aaagtccaaa tcggtacggc tgaaaaaccg taccgaaagt aaaaatctta
     5641 atttaatttg tcaacagcct tttttaagcc gaaaaccttc tttgcaactc cgtcaaactg
     5701 ctttggagta agtgactgcg caccgtccga aagtgcgtgc ggcggatcgt tgtgaacctc
     5761 tatgataaga ccgtctgcac ctgcggcaac tgctgccatt gcaagcggtt caacaagcca
     5821 tgacttgccc gatgcatggc tcggatcaac aataacagga aggtgagaaa gctttttgat
     5881 aatcgggata gccgaaatat caagagtgtt tcttgtaaat gtttcgtatg tgcggatacc
     5941 tctttcgcag agcataacct tatcgttacc gcctgccata atatattcgg cactcatgag
     6001 ccattcttcg attgttgcgg aaagacctct cttgagaagg ataggcttgt caattttgcc
     6061 gagctccttg agaagctcaa agttctgcat atttcttgca ccaacctgaa taatatccac
     6121 attctcgaac atatcaatgt gtgatgctct cataatttct gttacaatcg gcagacctgt
     6181 ttctcttctt gctgttttga gaagatcaag tccttctgcc ttgagtccct ggaaagcgta
     6241 tggtgaagta cggggcttga atgcgccgcc tctgagaagt gttgcgcccg atgctttaac
     6301 atcctttgca atctgaacaa tctgctcctc gctttcaaca gagcagggac ctgccataat
     6361 gtgcagactg ccgtcaccga ttgttacatt gtcattgatt ttaatgactg tattgtcggg
     6421 gtggaacttt ctgttagcct ttttgtacgg ctcctgaact ctctttacat tttcaacaat
     6481 gtcctgagcg tcaatatact caatgtcaat ctttgttgta tcgccgataa gaccgagtac
     6541 ggttgaatga acaccgtccc acttattaac ctttacatca tagctctctt tgagcatatt
     6601 tgtgaatttt gtaatctgct cctctgttgc agacggtttt aatactataa tcattgcttt
     6661 aatttccttt caattcactt ttgacgaaac gaatcgaagt tcgtcatatt tctttagtca
     6721 ttttagcact ttaaagcgtg atagtcaata gaataaatgc agaatcaagc gtttttatgc
     6781 ggtttttcag ccacatctgt tccgcttact gcgtcgatta tcgcaaaagc ggtttttaac
     6841 ggcgtatttt tgccctttac cacaatgtcg gcagcggcat gataaatcgg atatctgctg
     6901 tcataaaggt ctttcataac cctgtccctg tccggacgct gaagcagtgg tcttgttgtg
     6961 tcgtttttaa gacggctttt gatgattccc agcggaacat caagaaaaac aatcttgtcc
     7021 ttgcccttga aggcctcaac atttctcttg aatgtaaacg caccgccgcc cgatgcaatt
     7081 acaagacctt caagccctgc aagctcaaca caggcgtcat gttccatatc acgaaacttt
     7141 tcttcgccgt attttacaaa tatttcggaa actgtcattc ctgctttttc ttcaatgtat
     7201 gaatccatat caaggaattt tcttcccgat tttcttgcaa tgtttttgcc tacggttgat
     7261 tttccgcaac ccataaatcc gcataaaact atgttgttca ctttaaattt ctccgttcat
     7321 cagaaattct tttcaagctc ttttgcagag tcgatacaaa gctgagcaat gtcgcccttg
     7381 tcataaacag agccgtccca atgttcgtga gaaacaactg cctgccatac gagcattgcc
     7441 attccgcttt cggctgttgc accgtttgcc cttgcctttt taacaagcat tgtttcaagc
     7501 ggattgtaaa cagcgtcaaa tacatttgca caacggttta ttacactgtc gcttacaggc
     7561 tgattttcaa tcttaggaaa cattccgata ggagttgcgt tgataagcac atcaatgtca
     7621 cccgaaagtt cgtcaataag gcagaagctt acatcgcaac ctgcaactgt ttttctgatt
     7681 tcatcgcata aagcctcggc aattttcaaa tcctcttttc ttacggcaaa gaggaggggc
     7741 tgttttttga gaacaacctc atacgccatt gttcttgcaa cgccgccgca gccgatgatt
     7801 acgattctgc cgttaagcat aattcctgcc gcatcaagag cctttaaaaa tccgtccgga
     7861 tctgttgtgt aaccctttga aatgccgttt ttgtttgaaa ccgtgttcac actgccgtac
     7921 atctcggcct ttgcgtcaat ttcgtcaaga aacggaatga ctgcctgctt atgcggaatt
     7981 gtaatgttaa atccgtcaag ttttgaaagg gtatttttaa attcatattc aagattttcg
     8041 ggagcaatgt caagctttgt atattcggct ttgacgccct gaagttcaaa aagtcttgtg
     8101 tgtataaacg gtgacattgt gtgaccgatt gggtgaccga ttacggcaaa tttcttggtt
     8161 tccataataa aaaaatacct ccgaggttca tatttttagc tatgcagaaa taaacttaaa
     8221 agaaattatt tatgcaaaaa tcagaaattt ttcaaaaaaa tttcaaatat atattgacaa
     8281 aggggtaaat aatcaataat ataagggaag tgaagcaatc cgtttgcttt cataccattg
     8341 tagcataaac taagtgcttt tgtctataat ttactttgga caatcaatat ttttaccggg
     8401 aggaattatt aatggtatta gaaaaagtta aggcaattct tgctgaacag ttcgatgttg
     8461 aagaggataa gattactgcc gacacagatc ttcaggaaga tctcggcgca gattcactcg
     8521 atgttgttga ccttcttatg tcaatcgaag acgagtttga ggttgaagtt cccgatgagg
     8581 aaatcgaaaa catcaagaca gttggttcac tcgtttctta catcgaggct aactcataat
     8641 ttgctcttgt tttttcgcac ttaatttttg agaggtcgtt tttacggcct ctttttgttt
     8701 tgctcaaaaa ggttgaaaat ttgcagattt agtgtatatt gaaaaggctg aaaatttaaa
     8761 aattcagcct gtatcaaaaa ggttgaaaaa taaaagaatt agtgtataat agtaatgtta
     8821 tagatgttat gacagccgtg tattcggcat tttgaaaatg caaaaaaata tgttaaaaaa
     8881 taagtaaaat tttattttac ggagggaata taatggcaaa caacaaaaaa atggttgaag
     8941 ctatcacaag cagagatgag gactttgcaa aatggtacac cgacatcgtt aaaaaagctg
     9001 agctcgttga ctattcgggc gtaaagggtt gtatggttat ccgcccctac ggctatgcta
     9061 tctgggaaaa tattcaggct gaccttgacc gtcgtttcaa agaaacaggt cacgagaatg
     9121 tttatatgcc tatgtttatt cccgaaagtc ttttacagaa agaaaaagac cacgttgagg
     9181 gctttgctcc cgaatgtgca tgggttactg taggcggtca ggaaaagctt caggagcgtc
     9241 tttgcgttcg tcctacatcg gaaactcttt tctgtgaaca ttataaaaag attatcaaca
     9301 cataccgtga tcttccgaag ctctacaatc agtggtgcag cgttgtaaga tgggaaaaga
     9361 caacccgtcc gttccttcgt acatcggaat tcctctggca ggaaggtcat acaatgcatg
     9421 aaaccgaaga agaagcaaga gaagaaactc tcagaatgtt acatgtttat tctgatttct
     9481 acaaggaaac tcttgctatt ccggcagtaa tcggcagaaa gacagaaaaa gaaaagttcg
     9541 caggtgccga agaaacctac acaatcgagc cgatgatgca caacggtgtt gctcttcagg
     9601 gcggtacatc acactacttc ggcgacggct ttgcaaagag ctttggcatc acatacacag
     9661 gcaaggacaa caagcttcat tatccgcatc agacatcatg gggtgtttct acaagaatga
     9721 tcggtgcgct cattatggtt cactctgatg atgacggtct tgttcttccg ccgaagattt
     9781 cccctattca ggttgcactc attcctgttg cacagcacaa agagggtgtt cttgaaaagg
     9841 cagaggagct tagaaaggca cttgccgaaa agttcagagt aaaacttgat tcttcagacc
     9901 acgctcccgg atggaagttt gccgagtatg aaatgaaggg cgtgcctgta agagttgaaa
     9961 tcggtcctaa ggatattgaa aagaaccagt gcgtggttgt ccgccgtgat acccgtgaaa
    10021 aggcatttgt ttcaattgac gagcttgtac cgtttgttga aaaccttatg gttacaatcc
    10081 acaacgatat gtatgaccgt gctcttaaaa acactcagga aaagacattc acggcaacaa
    10141 catttgatga gttcgttgac acagccaaga acaagcccgg ctttatcaag gctatgtggt
    10201 gcggcgatac agcctgtgaa gaaaaaatca aagaagttac aggcggtgta aaaagcagat
    10261 gtatcccgtt caacgaggag catctctcgg atgtttgcgt atgctgcggc aagcctgcaa
    10321 agcatatggt attctggggc aagcagtatt gatgtataaa taatactttt tgtgtaaaaa
    10381 catagtaagg tggtgagaaa atgccaatca aggtacaaag taatcttccg gcaatcaagg
    10441 tgctggaatc ggaaaatatt tttgtaatgc cgaacgaggt tgctctcagg caggatatcc
    10501 gtccgctgaa aattcttatt ctgaatctga tgccgacaaa aatcgaaacc gaaacccagc
    10561 ttttaaggct gctcggcaac agtcctctgc aggttgatat cgagcttttg cagatggcat
    10621 cacacacctc taaaaacacc tctccgcatc acctgacaac tttttacaag acatttaatc
    10681 aggtgaagaa cgaaagcttt gacggaatga ttatcacggg tgcgcctgtt gaacagctcg
    10741 actttgaaga ggtcgattat tggggtgagc tttgtgaaat tatggagtgg tcaaagcata
    10801 atgtgtgttc aacattccac atctgctggg gcgctcaggc aggtctttat tatcactacg
    10861 gcataaaaaa gcaccttttg cccgaaaaat taagcggaat ttacacccac aaggtgcttg
    10921 ttccgcatca caagcttatg agaggctttg acgatacatt cacaattccg cattcaagac
    10981 atacgggaat tgatgaagag gcgttgaaaa gatgcagagg gcttgaagta cttgcggttt
    11041 ccgagattgc aggttcatgc gtaatttgca gtcgaaacgg cagacaggtg tttgttacag
    11101 gtcacgcaga gtatgaccgt gacacccttg ccaaggaata tttcagagat aaaaacaagg
    11161 gactcaatcc gaatgttccc tacaattatt ttccgaatga cgatgcgtca aaaactccgc
    11221 cgatgatttg gaggagcaac gcaaccttgc tctacacaaa ctggctcaac tatttcgttt
    11281 atcaggcgac tccgtatgac ctccgtgagc ttatcaacag atatcctcac taaattaaat
    11341 acaaaatcaa ccccgaagtg ccaaaacttc ggggttttgt tatgtaaagt gttgtattta
    11401 tcgtgtacat tatgttataa ttattttaat tgtacataat gaaaaagtgc aaaaatgaaa
    11461 atctgcaatg aaacaggtgt ttttatggag aaaaagcgta ccgtaatcaa ggttggtact
    11521 tcaacgctga cttatgaaaa cggaaaaata aattacagaa gagttgaaca gctttgcaag
    11581 gtgctttccg atttgcagaa ccgcggagaa caggttatct tcgtttcttc gggtgctatt
    11641 gctgtaggaa tgggcaaggc aggtcttgac aagcgaccta ccgaaaccaa gaaaaaacag
    11701 gctcttgccg caatcggtca gtgtgagctt atgtttatgt atgacaaact tttcggcgag
    11761 tacaaccact ctgttgctca gctgctgctt acccgccacg ctgttgaaac agagcagaaa
    11821 aggcagaatg ttatcaacac aattgacgag cttctcagaa tgaacattat ccccgtaatc
    11881 aacgaaaacg ataccgttac gattgatgag cttgagggca acaatttcgg tgataacgat
    11941 atgctttccg caattgtcgc aggacttgtt gccgcagaca gactcattat tcttacggat
    12001 attgacggtc tgtacgattc aaacccacga acaaatccaa atgccaaaaa gcttgatatt
    12061 atcgaaaaaa tcaatgcgga aatttttgaa atggcggcag gtacaggctc aaaccgtggc
    12121 acaggcggta tgattacaaa gcttcaggcg gctgattatg ccacaaaacg aggtgttgag
    12181 gtcagcgtta tcaacggctc aaatcccgaa aatctttatg aagtaattga cggaaacaaa
    12241 atcggaacaa aatttttacc tgttaaataa tcggaggaaa attatgacta cacttgaaca
    12301 aatgggcgca aaggcaaaag atgcgtcaag atttcttatg acagccggtt caaaaaagga
    12361 cattgccctc ggtgcaattg cagacgcact ctgcgaaaac tgcgaagaaa ttcttgcagc
    12421 taacaaaatt gaccttgaga acggcgaaaa aagcggtctt acggcatcac ttctcgacag
    12481 actcagattg tcggaggaga gaatcaacgg tatggctgac ggggtaagac aggttgcggc
    12541 tttacccgat cccgtgggca gagttcttga cggcagaacc ctcaaaaacg gcttgcagat
    12601 tgaaaaggta acagttccga tgggtgttat cggaatcatc tttgaggcaa gacctaatgt
    12661 aacttccgat gcggcggctc tttgccttaa agcaggcagt gccgtaattc ttcgtggcgg
    12721 taaagaggct tttcattcaa atatggcaat cgcaaaggtt atgcgtgacg cacttgaaaa
    12781 ggcaggattt ccccgtgact gcgtagcact tgttgaggac acaacaagac agagcgcaac
    12841 agagcttatg cagctttccg attatctcga tgtgctcatt ccccgtggcg gtgcaggact
    12901 tatcaaaagc gttgttgaaa atgcaaaggt tcccgttatc gaaacaggcg tgggtaactg
    12961 ccatatctat gttgacaaga gtgcagatat tgaaatggcg aagaatattg ttttcaacgg
    13021 caaaacctct cgcccatctg tgtgcaatgc gcttgaaaca ttgcttgtac acaaggatat
    13081 cgcagaaaaa gctctgcctg taatcaaagc agagcttgat aaaaagaatg ttgaaatcag
    13141 aggctgtgac agaacaaaac agattctcgg cgactgcgtt gttcctgcaa cggaagaaga
    13201 ttatagaatc gaatttctcg attacatcat tgccgtaaag gttgttgaca gccttgatga
    13261 cgcaattgct cacattcaga agtacagtac aggtcacagc gaatgcattg tgacaagcga
    13321 ctacaacagt gcaaacgagt tcacggcacg aattgattct gcggcggttt atgtaaacgc
    13381 atcaactcgc tttaccgacg gcggagaatt cggactcggt gcagaaatcg gaatttcaac
    13441 ccaaaagctc cacgcaagag gacctatggg acttaacgag cttacaagct caaaatatat
    13501 catcagagga aacggacaaa tccgataatc acatcagaag atacatcagc gcaagcagaa
    13561 ctgacggatc ggtgttttcc tccataagca gtatcacaag caaaagaatt aggctctgtt
    13621 ccttgttttt gaacatcaga ttcaaaagac cgtcatcctt tttcggaacg ggcggttcgg
    13681 gaatttcgga ctttggttct tcctgctttt cgggttgttt cggaacttcc cttcttggtg
    13741 cttcacgatt gaattgcggc tttcggtgca tctgctgtgc tcggttaaaa gcctcctctt
    13801 caaaactcgg caaaaaatca cctcctgtca gaacaatccg ttttcacgca gtaacggcat
    13861 aaggcggaat aatttcagca tcttttcggc tttgtcaagc ttttcctgcc gttcatgtga
    13921 aagaaacggt ctgagcgaat caagcagtcg ggtggtgtca tcgctgttgt tcagcgagct
    13981 taaaagcggt gcaaatttgg caattgtgcc gattgtgtcg tcaccgccca gcactgacat
    14041 aaggttattt tcgggcggtt tcggctttgg cggttgtttt tgctcacccg tcagaccaag
    14101 ctgtgcccca agactttgca cctgtttcat tgcctcgggg ttctgcaaaa tctgattgag
    14161 cttatcctgt aaatcagaca tatcattcac cgccgagcag tttcatcagc gcctgagcct
    14221 gcggactttg caaaagtttc tttgcttttt cgggatccga cagaacgctc tcaatctgct
    14281 ttgattgatt cggatttgta tttttcaaaa gattttctat gttaccgctt tgtgcggcac
    14341 tttcaagctg ttccttagag ataccgtact ttttcgatac ctcaccgagc agtttattaa
    14401 cttttttatt atcagacata ccaacacctc aacatatact atgcggaaat tttattttta
    14461 gaacggagaa ttttcaatgc gtattttggt agtttcggac actcacggag atcttcgcag
    14521 tcttatcaaa gccgttgacg cacagcgcaa ggcggaaata attgttcatt gcggtgacgg
    14581 tgaagaacaa cagcggtttt taaaagataa ttacaaagac aaaatgattg ttgccgtaag
    14641 gggcaactgc gattggaaca gcttttttcc gtcaaaggag atttttaaag cctgcggtaa
    14701 aaccatcttt gtaacacacg gacatctcta tgacgcaaaa tcgggacttt accgcattac
    14761 ctccgccgca agagaagcag gagcggatat tctcctcttc ggtcatacac atatgcctct
    14821 cacactctat gaggacggtc tgtatattat gaaccccggt tcatgccacg gatactatgc
    14881 gtcctacggc attatcgaca tcaccgacaa gggcgaaatt gttaccaata ccgtagcctt
    14941 aaaataattg tggaaattgc aaaaataaaa tgatataata aatgtgataa ataagcggag
    15001 gtgggatttt gaattttgac ggttttgatt ttgacgacaa tataaaacaa accttcaaca
    15061 cccttgatat tcagggcaga gttccccacg ctgttgtaat agaaagcagg gacagcgaaa
    15121 aggcgatgaa ccttgccgtg tttctgtcaa tgtatgcggt gtgtaccgaa cccgtaaagc
    15181 cttgcggtca ttgtgagcaa tgtcacaagg caaagaataa ggcacacgcc gacattaaat
    15241 atgttgaact tccaaatgac agaaaacaaa attcaattga gcagatgaga gagataatcg
    15301 gtgactctgt cataaagccg aatgaggcag atgcaaaggt gtatatcttc ccttttgccg
    15361 acacaaggct ttcggacatc gttcaaaact cgtttttgaa acttcttgaa gaacctccgc
    15421 agaatgtata tttcattatg ctctgcgaaa actccaagaa actgctcccg acaattctct
    15481 cccgttcaag tgtattccgt ctgaaaacaa aagatgtttt cagtgaggat gcagttgcag
    15541 gtgcaaagaa aattgtaaaa ggaattttat cgacacggga gtacaacctt atgcaggcac
    15601 tctatgccct cagcgacaaa aaccttgcag acgaaattct gcttgtcgta aagctgattc
    15661 tgcgtgacgg aatggcaaaa agcgttggag cggatgcggt ttttgacgag gaatgtgccg
    15721 gggagcttgc aagacgattt actcgggcaa agctgatttc tatgattgag cttaccgaga
    15781 acgcaaaact gaaaataccg aaacacataa atataaatct tctcacaact tggctgtgcg
    15841 gagaatacag gaggatttca tggcagagat aatcggagta cgatttaaag aagtcggcaa
    15901 agtgtattac tttgatccgc ttgacaataa acttaacaca ggcgacaggg taattgttga
    15961 aacggcaaga ggtcttgagt gcggagaggt tgcaactccg aacaagaccg ttgacgatgc
    16021 ggaaatttcg catccgctca agccgttaat cagaattgca accgaaaaag acctgaatca
    16081 ccttgcggaa aacaaactca aagaaaagga agcatacaga atctgtgaac agaaaattgc
    16141 caaccacaag cttgaaatga agcttgtaaa tgttgaatac acatttgaca acagcaaaat
    16201 tcttttctac tttacggctg acggacgagt tgacttccgt gcacttgtaa aagaccttgc
    16261 aagtgtgttc agaacaagaa ttgagcttcg ccagattggc gtgcgtgatg aggcaaaaat
    16321 gctcggagga ctcggcatct gcggtaagcc gttctgctgt gcaagcttta tgggcgagtt
    16381 ccagcccgtt tcaatcaaaa tggcaaagga gcagggcctg tcactctctc ccgttaaaat
    16441 ttcgggaacc tgcggcagac ttatgtgctg tcttaaatat gaacaggagg catacacaga
    16501 ccttcttaaa cacaccccaa aggtaggagc aattgtaaat actcccgaag gcagaggtct
    16561 tgttgttgaa aacaacctga ttgcaggaac tttaaaggtt aagctcaaca acactcccga
    16621 agatgccgct ccgaaatcat tcacagtcaa gcaatgcaag cttgttaagg acggctacat
    16681 caagcttgac aaaaaggaaa tggaaaaatt caagggactt gaataatttt ttgtaatgcg
    16741 tacagcgttc gtcaagttgc agtaaaaaag aactgttttc agtttaaaat ataaaattct
    16801 ttccaaaagg ttgctttttt caaaatttat gttacaataa aggtgaaatt catgaaggag
    16861 gtatttccct atggcatata ctatcagtga cgattgcatc atgtgtggtg cttgtgcaga
    16921 caactgccct tgcgacgcta tttcagaagg cgacggcaag tatgaaatca atgcagacgc
    16981 ttgtgttgat tgcggttcat gtgctgaagt ttgccccgtc ggcgctccac aggaaggctg
    17041 attaagggtt aatccacaaa gacaggcggc agggatttcc ttgccgcttt ttaattactg
    17101 ttttcagggg aataccgatg aacaataaac atcttgaacc gcttggcagc ggtattaaaa
    17161 tatatgtgtc agacagctac cgtttttcaa cggacacaat tttacttgcc gatttttcaa
    17221 agcaaatcgg tgcaaaaaaa tgtgttgacc tcggcacggg ttgcggaact attccgcttt
    17281 tgtggtacaa aaacaacaac cgccttgaca tcacggcggt agaaatacag gagaatgcct
    17341 gcaagctgtt ttcacaaagt cttgaataca acaatcttca ggacaaaatc agaattgtaa
    17401 actcggactt aaacaagctt gacggtgcat tgccgctcgg ctcgtttgac cttgttgcct
    17461 gcaatccgcc ctacaaaatc agcggaggcg gaattacaaa tcccgagaac gcaaagcttg
    17521 ttgcaaggca cgaatccgaa tgtacgcttg acgatgtgtg ccgttgcggt tcgtcgcttt
    17581 tgcagtttgg aggacggctc tgcatttgtc agcgccccga aagacttgcc gatgcaatgg
    17641 aggcaatgcg taaatattcg gttgagccga aaaaattaag gcttgttcaa cagcgaaagt
    17701 caaaagcccc gaagctcttt ttgcttgaag gcagaagggg cggcaaaagg ggatttcttg
    17761 aggttatgcc aaccctgttt attgaggacg aaagcggaaa cttttccgaa gaaatgctga
    17821 acatctacgg cagttacaag gaggactata taaattgagc ggaattttat atgtaacggg
    17881 aacgccgatc ggcaacctcg gagatttttc accgagagcc gcagaaaccc tcgaatcggt
    17941 tgatttcatt gcggcagagg acacaagggt aacgcttaag ctcttgaatc atttcggcat
    18001 aaaaaagcct atggtgagct attttgaaca caacaagcgt gagcgtggcg aaatcatctg
    18061 caacagaatt gaacagggcg aaaattgtgc aattgtaacg gacgccggaa tgccgtgcat
    18121 ttccgatccg ggtgaagatt tgataaagct ctgcgaggag cgtggaataa aaaccgtggt
    18181 gattcccggt ccgagtgccg tgatttccgc acttgcggtt tcgggacttg aaacaggcag
    18241 attcacattt gaaggctttt tgagcgtgaa caaaaagagc cgcaacgagc atcttgacag
    18301 tctgaaaaac gaacacagaa caatgatttt ctacgaagcg ccacacaagc ttccgcaaac
    18361 tctccgagat ttgtacaact ttttcggtga cagaaagctt tcgattgttc gtgaactcac
    18421 caaagttcac gaagaagtaa tccgcaccac aacaaaatat gctgcggaaa attatgcaga
    18481 cggcagtctg aagggcgaat ttgtgcttgt gcttgagggc aaaaagcaag aggaaaaatc
    18541 cgaagaatac acccttgaat ttgcggttga aaccgcccgc aagctgattg aaaacggtgc
    18601 aaagcccacc gaggcggcaa aagaggcggc aagtataaca ggctttaaga aaaatgagat
    18661 ttacagggag ctgttgtaaa tgacatacga aaatgctgtt gaaaaaattc attcactttt
    18721 gacattcggt tctcgtccgg gacttgaccg aatgagaatt ttacttgaca ggctcggaaa
    18781 tccgcaggac aggctgaaat ttatacacat tgcaggtacg aacggcaaag gctcggtttg
    18841 tgctatgctt tcatccgcac ttgttgcggc aggctacaaa acaggcttgt tcatttcacc
    18901 gtatatcacg gattttcgtg agaggataca aataaacaat tgtatggtga gcgagtccgt
    18961 tctcacggat gcggttgaaa aaactttccc tattatcgag caactgcgtg aagagggcac
    19021 aatcatcaca gaatttgagt atgtcaacgc tcttcaattt tatatacacg caaatgcaga
    19081 ttgtgacatt gttgttcttg aaacaggaat gggcggtctg ctcgactgca ccaatactat
    19141 aaaatcaccg ctttgtgccg ttatcaccac aatcggactt gaccacacgg caattctcgg
    19201 tgatacaatt gaaaaaattg cacagcaaaa atgcggaatc ttcaagcccg actcaatggc
    19261 ggtcacttca aagcaggata tccgtgcaat gaaggttatt gaatcaactg caagagaaaa
    19321 gaaaattccg cttgcaacga gcgagagcgt taatattgat gtcatcgaaa ccaccttaaa
    19381 gggcagtcgc tttatgttta acggtgttga aattgatctt ccgctttcgg gtgatcatca
    19441 gcttgaaaat gcaaaaaccg cacttgcgac acttgatatg ctccgttgca attctctgat
    19501 ttcaataaca gatgagcaga ttgcaaacgg ttttgcaaag gctgtcaacc ctgcaagact
    19561 tgagctgttg agtgaaaagc cgattgttct gcttgacggt gctcacaatc ctaacggaat
    19621 tgaggcgctt aaatcggcaa tccaaaaatt ccttccgaat aagtatataa ttgcaatcac
    19681 gggtatgctt gccgacaagg atgtcagcac ttcggtaaaa ctgcttgacg gagtttttga
    19741 cagagtttac acagttcccg ttcacaatcc aagggcaatg caccctgcaa agcttatgca
    19801 acagtatgca agatttgttg acgatacggt aacctttgaa agtgccgaac acgcatttga
    19861 tatggctttt gaacaggctg aaaagaccga ttgtgcagtt gtaatctgcg gttcgctcta
    19921 ccttgcaggc gaaatccgtc cgtatatcat caataaaatt aaggctgaaa acgaatcagc
    19981 cgaaaagtaa atttttcgtt acaacagaat aaaacaaatt ttttgctccg cacttattat
    20041 cattaagata acaggtgcgg agctcttatt ttttagaagg actgataaaa ttgctgaaaa
    20101 cggaatttaa aaataatatt ctcacggcat acattgacgg cgaaattgac cacgattcgg
    20161 cggcaggaat acgcacaaaa attgacgccg ccgttcagac aaacaaaccg aaacttttgt
    20221 gccttgattt ttcaaaagta tcgtttatgg actcatcggg cataggtctt gtaatggggc
    20281 gataccgcca gatgaagctt gtcggcggag cattgcgtgt gattaatatt cccgacagat
    20341 tgtacaggct tttttcaatg tcgggacttg aaagtttggg ggtgttgaga tgaaaacagt
    20401 aaatgaaatg aaactttgcg tgccgtcaaa aagctgtaat gaggcttttg cacggtgcgt
    20461 tgtgtcggca tttgtaatga gccttgatcc taccgtaaac gaactcagcg acctaaaaac
    20521 cgctgtgtcc gaggctgtga caaactgcat tgttcacggc taccgccacc aaagcggaac
    20581 gatttacata aacggaaaaa ttacctccga aaacaaggtg ataatcacaa tccgtgacaa
    20641 aggctgcgga atttccgata ttaaaaaggc aatggaaccg ttgtttacaa ccgctcccga
    20701 ggaggacagg gcaggactcg gctttgcagt tatgcagagc ttttgcgaca aggtgcgtgt
    20761 ccgttcaaag cccgacagcg gaacaaccgt aactcttgaa aaaattatag gttcggaaaa
    20821 tgaccgataa aaataaactt gccgacaaaa atatggggct tgtccatgcc tgttgcaaac
    20881 gattttcagg caaaggaata gagtacgaag aactttttgc atcgggttgt ctcggactcg
    20941 caaaggcgat taataacttt gacgaaagcc gtaacctgca gttttcaacc tatgcctttc
    21001 ctgtaattat gggcgaaatt aagcgacttt tccgtgacgg cggtgcaatc agggtcagcc
    21061 gtacattaaa ggagctttca cttgcaatat cgaggcttaa caatgaaagc aagctgaaaa
    21121 acggcagaga actcaccgtg agcgagcttg ccgaaaggct tgatacaagc gaggaaaaaa
    21181 tcattgacgc actcaacagc acaagagttc ctctctccct gactgccgaa tatgacagcg
    21241 agggcaaccc acagcttgat gtccctacag atgatattca agacgaaatc tcggaacgat
    21301 tgtcgcttga acaggcggta aaatccctat gcgaaaccga ccgaaaaata attcaactgc
    21361 ggtactatcg gagcaaaaca caaactcaaa ccgccaaaat tctttccatg acgcaggttc
    21421 aaatcagtcg gcgggaaaag aaaattctca ccgccattcg ggacaaaatg tcggtttagc
    21481 accccgattt tccgaatgtg gcgttcaatg aatcccctgc atatttttat ttataaaaaa
    21541 ttttcatatt taacgacaaa agcgcccgaa taacggacgc ttttactttt tctttaattc
    21601 tgtttttcaa gcttcagctt ttcaacaatt gcatcaaccg tggtttcgat actgctcagc
    21661 tccattgaca ccgtgatatc ggcatacttt ttatagagcg gataacgcac tctgtacaca
    21721 tcatcaagag tttcaccttt accgaatgca atgcctcttg ttttgatatt tgtaactctg
    21781 cgcttgattt catcgaggtc aacatcaatg aaaactacct tgccgttttt tctgaggttt
    21841 tccatggcat tttcactgag aaccattgaa ccgcctgttg cgataacggt cttgtcgtag
    21901 tcaagctctg caccgatttt tccctcaaga tgaagaaaaa agtcaagacc ttcattatca
    21961 agaatctcct gcaacggctt tcctgcctct ttgcagatta tcaaatctac atcaacaaag
    22021 gaataaccca gcgccttggc aagcaacaca ccgactgtgc ttttgcccga tcccggcatt
    22081 ccgattaaaa ctatattttt catattaaag aatccttaca ttaagtgaat ctgtgcttga
    22141 agtcgggcag gttttgtttg acgaaagcac aacaaccgtg tcgccttttt tgacaacgcc
    22201 tgtgctgagt gccttttcca tagcctgttt cgtgattgaa tcagatgtgc gtttttcttc
    22261 gcccatgaca gccataacac cccagttaag acaaagcttt ctgcacacaa gctgtgatgt
    22321 tgcaactgca ataacgggac agtcaggtct gtaatgaacc atcgctcttg caactcgacc
    22381 tgttgcactt tcaacaataa tagcctttgc gcctactgcc tgcgcaatat tctttgcagc
    22441 tttacagata cttgatctga ttgggttagt gtccttcatt ccgtgttctt ccacatactc
    22501 atgaagtgcg gtaaattcac cgttttcctc agcctccgaa caaattgcgt caagtgcctt
    22561 tacgctttca atcgggtact tacctgcggc ggattctgcc gaaagcatta ctgcgcttgt
    22621 gccgtcataa actgcattgg caacatcgct gatttcggct cttgtaggac ggggagccgt
    22681 tgtcatactc tcaagcatct gggttgcagt gattacatac ttacctcttg aaacaacctt
    22741 tctgatgatt gttttctgaa tttcaggcag cttgataaac ggaatttcaa cacccatatc
    22801 tccacgggca accataatac cgtctgcgta atcaatgatt gcgtccatat cgtcaacacc
    22861 gctctgattt tcaatctttg cgataatttc aacatactca tagccgaggc tgtcaataaa
    22921 gtcacgaaga gttctaacat catcgtgatt gcggacaaat gatgcggcaa caactgttgc
    22981 accgtggtta agaccgaact caatgtcctt tctgtcctga gcgcttacat acggcatatt
    23041 gataaagtag cccggaatgt tgatactctt gcggttttta agcacaccgc ccttgtttac
    23101 ggtacaaacg attgactcgg gatttaccga cttaacgaca agcgagataa taccgtcatc
    23161 aagcaaaagc tctctgccga ttgcctcctg accttcggat tcaaaaatat caacaagctt
    23221 cggaaacgaa aggcttacac ctgtttcgtc gccctcttct ttgtttttaa aaagactgaa
    23281 ctctgcaccc tcgacaagtt caacagaacc gtccttgaat gtgccgactc tgacctcagg
    23341 accctttgta tcgagcagaa ttgcaacatt ttgacc
//
LOCUS       DAOMVE010000011        22586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_372_length_22586_cov_3.682881, whole genome shotgun sequence.
ACCESSION   DAOMVE010000011 DAOMVE010000000
VERSION     DAOMVE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 22586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22586
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_372_length_22586_cov_3.682881"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1185)
                     /gene="rlmD"
                     /locus_tag="PLS64_01385"
     CDS             complement(<1..1185)
                     /gene="rlmD"
                     /locus_tag="PLS64_01385"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRL42518.1"
                     /translation="MSNRPETNKQSCPVYKKCGGCQLQNLSYPEQLEFKQRKVEKLLK
                     RFCTVEKIIGMKTPYHYRNKVQAAFYTDKKGKIISGVYQSGSHHVVGIDSCQTEDVIA
                     DKIIVAVRKLLPSFRMTTYNEDTGKGFLRHILVKRGFATNQIMLVLVTGTPVFPSKNN
                     FVKAILKQFPEITTIVQNINPYRTNLVLGDNQKVLYGKGYIEDILCGCRFRISPKSFY
                     QINPVQTEVLYGKAIEFANLKGNETVLDTYCGIGTIGIIAAKNGAGNVIGAELNGDAV
                     RDAIVNARANNLKNIRFYKADAGEFMREAADEDEKPDVVFMDPPRAGSDRKFLDSLIK
                     MSPKKVVYVSCNPETLARDLAYLTQNSPYKAQKIQPVDMFPHTAHIETVVLLSHKKPD
                     GHI"
     gene            complement(1370..2854)
                     /locus_tag="PLS64_01390"
     CDS             complement(1370..2854)
                     /locus_tag="PLS64_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HRL42519.1"
                     /translation="MGKPTGFMEYKREDAPAYSVKERIKNFDEFHDPLSKEDQQKQGA
                     RCMDCGVPFCQAGMQIGSAFSGCPLNNLIPEWNDLIYKGNWEQAYNRLKVTNNFPEFT
                     SRVCPALCEKACTCGANGDAVSVRANEYGIIENAYEEGLADARIPKVRTGKKIAIIGS
                     GPSGLAAADQLNQRGHSVTVFERNDRVGGLLMYGIPNMKLEKDVIERKINIMEEEGVT
                     FETCSNVGKDIKASEILKDFDRVILACGASNPRDIKVPGREANGIHFAVDFLKSTTKS
                     LLDCNLREGTFISAKGKNVMVIGGGDTGNDCVGTSIRHGAKSVLQLEMMPKLPDTRSA
                     DNPWPQWPRVCKTDYGQEEAIEVYGHDPRVYQTTVKQFIADKNGNLVGAELVKLKPEK
                     DAKTGRLMMKEVEGSEYKVDVELVLIAAGFLGSENYVTKAFGVETNARTNVATAEGSH
                     KTNVENVFVTGDMHRGQSLVVWAIREGRDVAKEVDESLMGYTNL"
     gene            complement(2868..7409)
                     /gene="gltB"
                     /locus_tag="PLS64_01395"
     CDS             complement(2868..7409)
                     /gene="gltB"
                     /locus_tag="PLS64_01395"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HRL42520.1"
                     /translation="MEQTVRENNKTLYSPKFEHDNCGIGAVVNIKGIKSHDTVANALT
                     IVETLEHRAGKDAEGKTGDGVGILLQISHKFFKKAAAEIGIKLGSERDYAVGMFFFPQ
                     NELARNQAKKMFEIIAEKEGLTVLGWRNVPSKPEVLGHRAVECMPAIMQCFIKRPHGV
                     KRGLPFDRKLYVARRVFEQSNEDTYVVSLSSRTIVYKGMFLVGDLRNFFLDLQDDDYE
                     SAIAMVHSRFSTNTNPSWQRAHPNRMIVHNGEINTIRGNADKMLAREETMRSEHLKGD
                     LDKVIPVVNTEGSDSAMLDNTLEFLVMSGMELPLAVMITIPEPWDNNGTMSQKKKDFY
                     QYYATMMEPWDGPASILFSDGDIMGAVLDRNGLRPSRYYITDDGNLILSSEVGALPID
                     PAKIVTKERLRPGKMLLVDTVQGRLIDDDELKEYYASKQPYGEWLDSNLVCLKDLKIP
                     NMKVEEHSYKERQKLQKAFGYTYEDVMTSILPMAKNGTESIAAMGTDSPLAVLSKEPQ
                     PLFNYFKQLFAQVTNPPIDAIREEVVTSTTIYIGEDGNILEERPENCKVMKIHNPILT
                     STDILKIKNMHIPGFKVAVIPILYYKNTRLSKAVKRLFIEADKAYNDGANILILSDRG
                     VDENHLAIPSLLAVSALHQHLVATKKRTSLAMILESGEPREVHHFATLLGYGASAINP
                     YLAQETIHELIGDDLLDKDYYAAVDDYNSAVLHGIVKIASKMGISTIQSYQGSQIFEA
                     IGIGKDVIDEYFTGTVSRIGGITIKDIEKNVDKLHTAAFDPLDLGVSDELESRGSHKF
                     RSGKEEHLYNPQTIYMLQQATRTGDYELYKKYSHMISEEMDPVNIRGLFDFNFAETPV
                     PLDEVESVDSIVKRFKTGAMSYGSISQEAHETLAIAMNQLHGKSNSGEGGESLERLLT
                     KGQKVDRCSAIKQVASGRFGVTSRYLTSANEIQIKMAQGAKPGEGGHLPGKKVYPWIA
                     KTRHSTPGVSLISPPPHHDIYSIEDLAQLIYDLKNSNKDARISVKLVSECGVGTVAAG
                     VAKAGAGVILISGYDGGTGAAPRNSIYNAGLPWELGLAEAHQTLIMNDLRNKVVIETD
                     GKLMNGRDVAIAAMLGAEEFGFATAPLVTLGCAMMRVCNLDTCPFGVATQNPELRKRF
                     CGKPEYVINFMKFVASELREYMSKLGVKTVDELVGRIDLIKRKEGITGQAAEVDLSNI
                     LDTNFVSEKVEYNKKSLYDFKLESTLDERILCKEFAKVLAKGTKKKVEVNVKNTDRSF
                     GTIFGSEVTRKHYNNLDDDTFTVVCNGSGGQSFGAFIPKGLTLELVGDSNDYFGKGLS
                     GGKLVVYPPKNSTFDAGNNIIIGNVALYGATSGKAFINGVAGERFCVRNSGATAVVEG
                     VGDHGCEYMTGGRVVVIGKTGKNFAAGMSGGVAYVLDEERDLYTKLNKEMVLFSEVTE
                     KYDIIELKTLIEEHVAATGSQRGKKILDNFDEYLPKFKKIIPHDYKRMMAAIAAYEEK
                     GLTNEQAQIEAFYEIVNNK"
     gene            8029..10122
                     /locus_tag="PLS64_01400"
     CDS             8029..10122
                     /locus_tag="PLS64_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRL42521.1"
                     /translation="MAKVPEIFGSMVFNDQKMQERLPKSTYKALKKTIQNGEPLDLSV
                     ANVVAAAMKDWAVEMGCTHYTHWFQPMTGITAEKHDSFIAPNGEGQVIMEFSGKELVK
                     GEPDASSFPSGGIRATFEARGYTTWDPTSYAFVKDGTLYIPTAFCSYTGEVLDKKTPL
                     LRSMERINTEAVKILHLLGKENVTRVTTTVGPEQEYFLIDKDAYDQREDLIYTGRTLF
                     GAKAPKGQELDDHYFGAIKTRVAAYMKDLDEELWKLGILAKTKHNEVAPSQHELAPIF
                     TTTNIATDHNELTMEVMKKVAERHGLVCLLHEKPFAGVNGSGKHNNWSISTNTGENLL
                     DPGKTPENNLQFQLFLAAVVKAVHEYQDLLRITVASAGNDHRLGANEAPPAIISMYLG
                     DDLGELVDSIINDREYVSKGKQKMRTGVDVLPDFMKDTSDRNRTSPFAFTGNKFEFRA
                     LGSSLNIACPNYMLNTMVAEELSEFYDELKDADDMDAAIKALVKKVFTEHQNIIFNGN
                     NYAPEWVEEAERRGLLNLKSLPDAMEHFLDKKNIDLFVKNKICSADEIRARYEIELES
                     YSKQINIEALTMIDMAKKNILPAVTSYVRDLTDTALAKKALSDAIPTSVEEDLITSLS
                     NKLVCFSKKTAELEEAVIKASDYSDDNLKYAKYYRETVFALMQELRAVGDAMETETAS
                     EYWPYPSYGELLFGV"
     gene            10256..11956
                     /locus_tag="PLS64_01405"
     CDS             10256..11956
                     /locus_tag="PLS64_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HRL42522.1"
                     /translation="MAELIYGGTGVWFMIGALLVFFMQCGFAMVETGFTRAKNAGNII
                     MKNLMDFCIGTVVFSLLGFGLMMSEDYVAGFIGVPNLNMFTDWMNFDWNSFVFQLVFC
                     ATAATIVSGAMAERTKFSAYCVYSAIISLVVYPIEAGWVWNGQGWLAQLGCIDFAGSI
                     VIHMVGGISALVGAIILGPRIGKYGKNGKVNAIPGHSITLGALGVFILWFAWYGFNGA
                     AAEDGARLGQILLTTTIAPAVATVTTMLFTWIKNGKPDVSMCLNASLAGLVAITAGCA
                     DVDAIGAAVIGIVSGILIVVAVEFVDIKLKIDDPVGAFSVHGVNGMWGGLAVGLFATG
                     NGQNGITGLFYGGGFAQLGKQALGIVTIVAWTVVCMIIVFTIIKKTVGLRVTKDEEMK
                     GLDICEHGLISAYADFMPIGVGTASLDETFDVDSNVPVTQAVPVQLAGKYDMATDGTK
                     ITKIDILLKQSKLEALKEEMDKLGITGMTVSQVLGCGAQKGKAEYYRGVAIESNLLPK
                     MKVEIVVCKVPVKAVVDAAVKALYTGHIGDGKIFIYDVEDVVKVRTGETGYDAMQDIE
                     "
     gene            12141..12704
                     /locus_tag="PLS64_01410"
     CDS             12141..12704
                     /locus_tag="PLS64_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL42523.1"
                     /translation="MTDYRKLCIDLFGTDNETELKDIAGRLKGGRKNKLTETDVKNAI
                     EMQKNGKTTAEIAQTFNVSRQTISKYLNKPLNGNYVMRLDFMFRQKVCTEIYVNFADK
                     KIKIVNRTNDIMKRAFGINENPDWNDFKQFLEERCFPKSRAFRKTILKKIGADGYDTL
                     QILEKTDGRTAEDNQYIRFTRKELYAF"
     gene            12701..13519
                     /locus_tag="PLS64_01415"
     CDS             12701..13519
                     /locus_tag="PLS64_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42524.1"
                     /translation="MIKEINLDNYSLIDTKSASSKGNQQKWLVNDFWYKADHMGYEGL
                     CEVIVSELLKKSNITDFAEYFPIKIEFAGRKLNGCYSKNFRQKNESIVTLEHLSKQWL
                     ASSMAKNLLKYDDVKDKISHTVDFIENKTGLKNVGKYITAMLELDAFFLNEDRHTNNI
                     AFILNDDTGEYRFCPYFDFGLSLLADTTEDYPLGEDIYNLIPKIHAKPFDVDFDVQLD
                     AAQELYGEQVKFSFMSSDIEKSFEKVSGYYPDEVVVRVKNLIYNQKQKYGNMFE"
     gene            13693..14262
                     /locus_tag="PLS64_01420"
     CDS             13693..14262
                     /locus_tag="PLS64_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HRL42525.1"
                     /translation="MKNALLISHSEQSAVSLSQLLGSEGYTEISVCQTSQEAKELANG
                     EVFDLICVNAPLEKENGIELSKYFAGTTRSSVVIIVPQRNADYVNDALTEHGVLVIAK
                     PVNKHLFHHFLQFTECFKMRMFRVIEENEKLKHMVADMKIINRAKFLLITCLNMSEDQ
                     AHRYLEKQAMDLRTSKLEVAKQVIRTYEN"
     gene            14300..15382
                     /locus_tag="PLS64_01425"
     CDS             14300..15382
                     /locus_tag="PLS64_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HRL42526.1"
                     /translation="MSRAAYLILENGTVFEGKAFGAEKETTGELVFTTAMTGYLETLT
                     DPSYYGQVVIQTFPLIGNYGVIPADFESDSPSLKGYIVREWCQVPSNFRCEGDLDTFL
                     KESGIPGIYGIDTRALTRIVREYGVLNCKISYSPDVTKEELDEIKNYVITEAVESTTI
                     KEKEHFDAENGDLNVVLMDFGAKHNIGRELVKRGCNLTVVPAHTTADEIKAMNPDGVM
                     LSNGPGDPSDNTEIIAELKKLCDFGIPTFGICLGHQLLALSQGAKTEKLKYGHRGANQ
                     PAKEVETGRVYITSQNHGYAVVSDSMPENGKVSFVNGNDNTCEGVNYTNMPAFSVQFH
                     PEACGGPHDTAFLFDRFIELMKNNRK"
     gene            15453..19505
                     /gene="carB"
                     /locus_tag="PLS64_01430"
     CDS             15453..19505
                     /gene="carB"
                     /locus_tag="PLS64_01430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRL42527.1"
                     /translation="MPLKKNIKRVMVIGSGPIVIGQAAEFDYAGTQACRALKEEGLEV
                     ILVNSNPATIMTDNAMADKIYIEPLTLETVKRIIKKERPDSLLSTLGGQTGLTLSMQL
                     AKEGFLDKYNVQLLGANPETIDKAEDRQLFKDTMIEIGQPVIPSLVVTDVESAVKFAD
                     EIGYPVIIRPAFTLGGTGGGIVDNEEELREITSNGLELSPITQVLVEKCISGWKEIEF
                     EVMRDSDGNVITVCSMENFDPVGVHTGDSIVIAPTVTLADKEYQMLRSAALNIISALK
                     VEGGCNCQFALNPETFEYAVIEVNPRVSRSSALASKATGYPIAKVAAKIAIGYTLNEI
                     KNAVTGTTYACFEPALDYVVVKFPKWPFDKFVYAKRTLGTQMKATGEVMAIAPTFEQA
                     IMKAVRGAEISHDTLNDKEFAELSNASVYDRLSVCDDRRIFCVYEALKRGITVEQIHN
                     ITLIDEWFLEKLKNLIAVENELKNGELTEDLYVRAKNKGFLDRTIEKITGRKVENPLV
                     PVYKMVDTCAAEFEAETPYFYSTFDKENEAEEFIREQKSDKKTVIVFGSGPIRIGQGI
                     EFDYASDHCVWALKKAGYDVVIVNNNPETVSTDFDTADRLYFEPLTTEDVMGIIKTEN
                     PFGVVVAFGGQTAIKLTKFLSDNGVNILGTSYDAIDMAEDRERFDELLEKYHIKRPRG
                     VTVMTTDEALDVADKIGYPVLLRPSYVLGGQNMIIAFNEDDVKEYMAIILAQNIENPI
                     LIDKYLQGTELEVDAICDGEDILIPGIMEHIERAGVHSGDSIAVYPAWNLSDRMTQII
                     IESSKNLAIELRTKGLVNIQYLIYKDELYVIEVNPRSSRTIPYISKVTGVPMVDLATR
                     AMLGEKLKDMGYGTGLYRKSPYIAVKVPVFSFEKLINVDNHLGPEMKSTGEVLGVAGT
                     LEEALYKGLIGAGYKMKKKGGVFITVRNSDKAEIGEIAKKYYDLGFRIYATEGTADVL
                     KKYGIDAVSVKKIHESKTNNTLTLIESGKIQYVISTSAKGRIPSRDSVKIRRKTVERN
                     IPCLTSLDTANALADCLKSHYSQHSTELIDINHMREEKLMLKFTKMQGIGNDYIYCST
                     FDQEISNPEALAVRLSDRHFGIGGDGIILVCPSKVADAKMKMYNLDGSEGKMCGNGIR
                     CVGKFLYDHGMVDINEKDEITIETLSGIKKLKAYTSGGKVNRLRVDMGKAILEPKEIP
                     VVLDGDKVVDRPVEIAGKNYNITCVSMGNPHCVVFMDDIDNLDIETVGPEFENDKLFP
                     ERVNTEFVTVLDDHTIKMRVWERGSGETWACGTGACAVAVAACENGFCKKGEDIKIKL
                     KGGDLIINYTDETVYMTGNAEKVFEGEVEV"
     gene            19508..21130
                     /locus_tag="PLS64_01435"
     CDS             19508..21130
                     /locus_tag="PLS64_01435"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRL42528.1"
                     /translation="MEITEHKKNKKYIFVTGGVVSGLGKGITAASLGRLLKARGLKIV
                     AQKLDPYINVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDEDLNKYSNLTTGKV
                     YSNVLEKERHGDYLGKTVQVIPHITNEIKSFIYNVGEKTNADVVITEIGGTVGDIESQ
                     PFLEAIRQVSLEVGRENSIFIHVTLVPYIKGSEEHKSKPTQHSVKELRSLGINPDIIV
                     LRCDEPLEESIFNKIALFCNVKPDCVIENITLPTLYQAPLMLEKSNFSNVVCRELNIV
                     TDKKPDLSEWEEMLSRINNRTKKCTIALCGKYVALHDAYLSVAEALRHGGYENSADVE
                     IKWVDCELLTKYKVDELLGDVDGILVPGGFGNRGIEGKIMAAKYAREHDIPYLGICLG
                     MQTAVIEYARDVLGYADANSGEFTPEGKHNVIDLMPEQEGIEDMGGTMRLGAYPCVIK
                     PDSLMAKAYGQHEISERHRHRYEFNNDFREEIENAGMKITGTSPNGLLVEAVEVPDNR
                     FFVAVQYHPEFKSRPNRAHPLFREFIKASLEK"
     gene            21174..22364
                     /locus_tag="PLS64_01440"
     CDS             21174..22364
                     /locus_tag="PLS64_01440"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRL42529.1"
                     /translation="MKINRNFDNLVPNYLFADVAKKTNAYIAAHPDKKVIKLGIGDVT
                     LPLAPVVVEAMKKGAEDLAHKETFKGYPDYEGYEFLRQAISDYYKSFGVAVGADEIFV
                     SDGAKSDCGNIGDIFSKDNVVLVTDPVYPVYVDSNIMAGREIVYATSNEANGFAALPD
                     ENVKADIIYLCSPNNPTGSAYNAEQLKTWVDYALKNDAIILYDSAYEAFITEDLPRSI
                     FAIEGARKCAIEMCSLSKTAGFTGTRCGYTVIPKELERDGHNIYATWYRRQATKFNGV
                     SWPVQCAAAAVFSEEGQKQIKENISYYQENARIIASALDELGIYYTGGKNSPYIWLKC
                     PNNMGSWEFFDLLLNEANVVGTPGEGFGENGAGYFRLTSFGDRENTIEAVERIKKVLT
                     NLSK"
BASE COUNT         6381 a         4738 c         5077 g         6390 t
ORIGIN      
        1 gatatgtccg tctggctttt tgtgggaaag cagtactacc gtctcaatgt gtgcggtgtg
       61 ggggaacata tctacgggtt ggattttttg ggctttgtag gggctgtttt gggttaggta
      121 ggctaaatct cttgccaatg tttcggggtt gcaggaaaca tagacaacct ttttcggaga
      181 cattttgatt agtgagtcga gaaattttcg gtcacttccg gctcttggcg gatccatgaa
      241 cacaacatcg ggcttttcgt cctcgtctgc agcttctctc atgaactcgc ccgcatcggc
      301 tttgtagaaa cggatatttt tcagattatt tgctcttgcg tttacgattg catccctcac
      361 ggcatcgccg ttaagttctg caccgataac attgcctgcg ccgtttttgg cggcaattat
      421 gccgattgtg ccgattccgc agtaggtgtc aagcacggtt tcgttgcctt tgagatttgc
      481 aaattcaatt gctttgccgt acaaaacttc ggtttggacg gggttaattt ggtaaaagct
      541 tttcggcgaa attctgaacc tgcaaccgca gagaatatcc tcaatataac ccttgccgta
      601 cagcactttc tgattgtcac cgagtacaag gttggtgcgg taggggttta tattttgcac
      661 aatggttgtg atttcgggaa actgtttcag aatcgctttt acaaaattgt tttttgacgg
      721 aaaaacaggt gtacctgtca caagcacaag cataatctga tttgtcgcaa agcctctttt
      781 cacaagaatg tgacggagaa aacctttgcc tgtgtcctcg ttataggttg tcattctgaa
      841 agaggggagg agcttgcgta ctgcaacgat gattttgtcg gcaataacat cctctgtctg
      901 acagctgtca attccgacaa catgatgact gcccgactga tacacacccg aaatgatttt
      961 gccctttttg tccgtataaa aagccgcctg aaccttgttg cggtagtggt agggagtttt
     1021 cattccgata attttttcaa cggtgcaaaa gcgtttgaga agtttttcga ctttcctctg
     1081 cttgaattca agctgttcgg gatatgacag attttgcagt tgacaaccgc cgcatttttt
     1141 gtaaacggga cagctttgtt tattggtttc aggtctgtta ctcataatat ttcctcacat
     1201 tttattgcat tgtatatatc ctttatttta tcatcaatca tattgatatg caacagaaac
     1261 aaaccctaaa tgacaaagaa aaacgcagga gggaaatccg ctcctgcgct ttctgattat
     1321 taaccggtat atcgaaaaaa ttgaggatta tcaggttatt tgtaagcaat taaagatttg
     1381 tgtatcccat aaggctttcg tcaacctctt tggcaacatc tctgccctca cgaatcgccc
     1441 agacaacaag tgactgtcct ctgtgcatat cgcctgtaac aaatacattt tcaacatttg
     1501 tcttgtgtga gccttctgcc gttgcaacat tggttcttgc gtttgtttca acaccgaatg
     1561 ccttggttac atagttttca ctgccgagga atcctgccgc aatgagtacg agttcaacat
     1621 caaccttgta ttctgagcct tcaacctctt tcatcataag tctgcctgtt ttagcatcct
     1681 tttcgggttt gagcttaaca agctccgcac ctacaagatt accgttcttg tcagcaatga
     1741 actgcttaac ggttgtctga taaactcgtg gatcgtgacc gtaaacttca atagcctctt
     1801 cctgaccgta gtctgttttg cagactctcg gccattgcgg ccatgggtta tcggcacttc
     1861 ttgtgtcggg aagtttaggc atcatttcaa gctggagaac ggattttgca ccgtgtctga
     1921 tgcttgtgcc gacgcagtcg ttacctgtgt cgccgccgcc gattaccatt acattcttgc
     1981 cttttgcgct gatgaatgta ccctctctga ggttgcagtc aagaagtgac tttgttgttg
     2041 atttgaggaa gtcaacggca aagtgaatgc cgttagcctc tctgccggga actttgatat
     2101 ctctcggatt tgatgcaccg caagcaagga ttacacggtc aaagtccttg agaatttcgc
     2161 ttgctttgat gtctttgccg acatttgagc aggtttcaaa ggttacgcct tcttcttcca
     2221 taatgttaat cttgcgttcg ataacatctt tttcaagctt catattcgga atgccgtaca
     2281 taagcagacc gcctactctg tcattgcgtt caaataccgt tacagagtga ccgcgctgat
     2341 tgagctggtc ggcagcggca agtcccgatg gacccgaacc gatgattgca attttcttgc
     2401 ctgttcttac ctttggaatt cttgcgtctg caagaccttc ttcataggca ttttcaatga
     2461 ttccgtattc gttggctctt acgctgacag cgtcaccgtt tgcaccgcag gtgcatgcct
     2521 tttcacagag agcaggacat acacgggatg tgaattcggg gaagttgttt gtaactttaa
     2581 gacggttgta agcctgttcc cagttgccct tgtagatgag gtcattccat tcgggaatga
     2641 ggttgttaag cggacagcct gagaatgcag aaccaatctg cattcctgcc tgacagaaag
     2701 gcacgccgca gtccatgcat cttgcaccct gtttttgctg atcctctttg ctgaggggat
     2761 cgtgaaattc gtcaaaattt ttgattcttt cttttacgga gtatgccggg gcatcctcac
     2821 gcttatattc cataaatcct gttggctttc ccataaatat cccctcctta tttattattc
     2881 acaatttcgt agaaagcttc aatctgtgcc tgctcgtttg taagaccttt ttcttcataa
     2941 gcggcaattg cagccatcat tctcttgtag tcgtgcggaa taatcttctt gaatttcgga
     3001 agatactcgt caaagttatc aaggattttc ttgcctctct gtgaacctgt tgctgcaaca
     3061 tgttcttcaa taagcgtctt gagttcgatt atatcgtact tttctgttac ctcagagaag
     3121 agaaccattt ctttgttgag ctttgtgtaa agatctcttt cttcatcaag aacataggca
     3181 acaccgccgg acatacctgc ggcaaagttt ttgcctgttt tgccgataac aacaacccga
     3241 ccgcctgtca tatattcaca accgtggtcg ccgacaccct caacaactgc cgttgcgcct
     3301 gagtttctta cgcagaaacg ctcgcctgca acaccgttga tgaatgcttt gccgcttgtt
     3361 gcaccgtaaa gtgcaacatt accgatgatg atgttgttac ctgcgtcaaa tgttgagttc
     3421 ttcggaggat aaactaccag cttaccgcct gaaagtccct tgccgaagta gtcgtttgag
     3481 tcgcccacaa gttcaagtgt gagtcccttt ggaatgaatg cgccaaagct ctgaccgccc
     3541 gaaccgttgc agactactgt aaatgtatcg tcatcaaggt tgttgtaatg ctttcttgta
     3601 acctcagaac cgaagattgt accgaatgaa cggtcagtgt tcttaacatt gacttcaacc
     3661 tttttctttg tgccttttgc aagaaccttt gcaaattctt tgcagaggat tctctcgtca
     3721 agtgttgatt caagcttgaa atcataaagg ctctttttgt tgtattcaac cttttccgaa
     3781 acaaagtttg tatcaagaat gttagaaagg tcaacctcgg cagcctgtcc tgtgatgcct
     3841 tcttttctct tgatgaggtc aattcttccg acaagctcat caacggtttt tacaccgagc
     3901 tttgacatat attcacgaag ttcgcttgct acaaacttca taaagtttat tacatattcg
     3961 ggcttgccgc agaatctttt tctgagctcg gggttctgtg tggcaacacc gaacgggcag
     4021 gtatcaaggt tacatactct catcattgca caaccgagag ttacaagcgg agcagttgca
     4081 aaaccaaatt cctctgcacc gagcattgct gcaattgcaa catcacggcc gttcataagc
     4141 ttaccgtcgg tttcaataac aaccttgtta cgaaggtcgt tcataatgag cgtctgatga
     4201 gcctcggcaa gaccgagttc ccacggcaga cctgcgttgt agattgagtt tctcggagca
     4261 gcgcctgtac cgccgtcata acctgaaata aggattacgc ctgcacctgc ttttgcaacg
     4321 cctgcggcaa ccgtacctac accgcattcc gaaacgagct taacagagat tcttgcgtct
     4381 ttgttagaat ttttgaggtc gtagataagc tgtgcaaggt cctcaattga gtaaatgtcg
     4441 tggtgcggtg ggggagagat gagcgataca cccggtgttg aatgacgggt ttttgcaatc
     4501 cagggataaa cctttttgcc cggaagatgt ccgccttcgc ccggctttgc accctgagcc
     4561 attttaatct gaatttcgtt tgcagatgtg aggtatcttg atgtaacacc gaaacgacct
     4621 gacgcaacct gcttgattgc agaacatctg tcaaccttct gtccttttgt gagaagtcgt
     4681 tcaaggcttt cgccgccctc accggagttt gacttgccgt gaagctgatt cattgcaatc
     4741 gcaagtgttt cgtgagcctc ctgtgagatt gaaccgtagg acatagcgcc tgtcttgaaa
     4801 cgctttacga ttgaatcaac gctttcaacc tcatcaagcg gaacaggagt ttccgcaaag
     4861 ttgaagtcga aaagacctct gatgtttaca ggatccattt cttcggaaat catatgagaa
     4921 tactttttgt aaagctcata atcgcctgtt cttgttgcct gctggagcat atatatagtc
     4981 tgcggattgt agaggtgttc ctctttgccg cttctgaact tgtgtgaacc acgggattca
     5041 agctcatcgc ttacaccgag gtcaagcgga tcaaatgccg ctgtgtgaag tttgtcaaca
     5101 ttcttttcga tatccttgat tgtgataccg ccgattctgc tgactgtgcc tgtgaagtat
     5161 tcgtcaataa catccttgcc gataccgata gcctcaaaaa tctgtgagcc ctgatatgac
     5221 tggattgttg aaatacccat ttttgacgca atctttacga tgccgtggag aactgcgctg
     5281 ttgtaatcgt caactgctgc gtagtagtcc ttgtcaagaa ggtcatcacc gataagctcg
     5341 tgaattgttt cctgagcaag atacgggttg attgcacttg caccgtagcc gagaagtgtt
     5401 gcaaaatgat gaacttctct cggctcaccg ctttcaagta tcattgcaag tgaggttctc
     5461 ttctttgttg caacaaggtg ctgatgaaga gcggatactg caagaagtga cggaattgca
     5521 agatggtttt cgtcaactcc tctgtcggag aggataagaa tatttgcacc gtcattgtaa
     5581 gccttgtcag cttcaatgaa aagtcgctta acagcctttg aaagtcttgt gttcttgtag
     5641 tagagaatcg ggataaccgc aaccttaaag cccggaatgt gcatattctt gattttgaga
     5701 atatcggttg atgtaagaat cgggttgtga atcttcataa ccttgcagtt ttcgggacgc
     5761 tcctcaagga tgttgccgtc ctcgccgata taaattgttg ttgatgttac aacttcctca
     5821 cggattgcgt caatcggcgg gtttgtaacc tgtgcaaaga gctgtttgaa gtagttgaaa
     5881 agcggctgcg gttcttttga aagaactgca agcggactgt ctgtacccat tgcggcaatt
     5941 gactctgtgc cgttctttgc cataggaagg attgatgtca taacatcttc gtaggtatat
     6001 ccgaaagcct tctggagttt ttgtctttct ttatatgaat gctcctcaac cttcatatta
     6061 ggaattttga ggtctttaag acaaacaaga ttactgtcga gccattcgcc gtaaggctgt
     6121 tttgacgcat aatactcttt gagttcatcg tcatcaatga gtctgccctg aaccgtatcg
     6181 acaaggagca ttttgcccgg gcgaagtctt tcctttgtaa caatctttgc gggatcaata
     6241 ggaagtgcgc cgacttccga tgaaagaatg aggttgccgt catccgtgat gtaatatctt
     6301 gacggacgca gaccgttacg gtcaagaacc gcacccataa tgtcaccgtc agagaaaagg
     6361 attgacgcag gaccgtccca cggctccatc attgttgcat aatactgata gaagtccttt
     6421 ttcttctggc tcattgtgcc gttattatcc cacggctccg gaattgtaat cataacggca
     6481 agaggaagct ccataccgct cattacgaga aattcaagtg tgttatcaag cattgcagag
     6541 tctgaaccct ctgtgtttac aacaggaata accttgtcaa ggtcgccctt gagatgctcc
     6601 gaacgcattg tttcctcacg ggcaagcatc ttgtcagcgt tgcctctgat tgtgttgatt
     6661 tcaccgttgt gaacaatcat tctgttagga tgagctctct gccagcttgg atttgtgttg
     6721 gttgaaaaac gggagtgaac catagcgatt gcagactcgt aatcatcgtc ctgaagatca
     6781 aggaagaagt tacggagatc gccgacaagg aacatacctt tatatacaat agttctgctt
     6841 gaaagcgaaa ctacatatgt gtcttcatta ctctgttcaa aaactcttct tgcaacatag
     6901 agctttctgt cgaacggaag acctcttttt acaccgtgag gtcttttgat aaagcactgc
     6961 ataattgcag gcatacattc aacagctctg tgaccgagta cctcgggctt tgacggaaca
     7021 tttctccaac cgagaactgt aagaccttct ttttctgcaa tgatttcaaa catcttcttt
     7081 gcctgatttc ttgcaagctc attctgaggg aagaagaaca tacctacagc gtaatctctt
     7141 tcggaaccga gcttaatgcc gatttcagca gctgccttct taaaaaattt gtgtgaaatc
     7201 tggagaagaa tacctacgcc gtcacctgtt ttgccttcgg catccttacc tgctctgtgt
     7261 tcgagagttt caacaattgt cagagcgttt gccacggtgt cgtgtgactt gataccctta
     7321 atattaacaa cagctccgat accgcagtta tcatgttcaa acttcggaga atacagagtc
     7381 ttgttgttct ctctgactgt ctgttccatt aatgatcacc ctttcctgtt aatcacaaaa
     7441 attattcagc ggacttttta tatacatata tatgtaaaag gaagtttgct ttcgtaaact
     7501 tatcggttca aagtggcaat taatgttctg ccggttaata atcaatcata tatatgtaat
     7561 tgtaaaatgc ccaatctctt ctcagcggta aaaccgctat gctcagcctg tgctatatcc
     7621 tgcaaaggtg aatgtgcagg tcggctgatg ttctgaaatg ccctgtcatc gttgatcaag
     7681 aaaaatcagc acttagatta tataactata cattgtgctt tgtcaaggga aaataaagga
     7741 aaattgcaac ttttaagggc aaaactttca ttttactgtg attgttgtag aaaatatgca
     7801 actcttattg gtatttcagt ttaattttgc aggaaattta ataaaacttg caatttcaaa
     7861 acttttttca aaaaatgtat tgacaaattc gcaaaagtgg aatatactaa caacatcaaa
     7921 gacagcaagg gcgctgtgag ctgaggctta cagtgtcctt ttctgttttt ataaagcaaa
     7981 aatcagacca ttaaaattta ttttcaaaat ggataggaga gaattaaaat ggcaaaagtt
     8041 cccgaaattt ttggcagcat ggtatttaat gaccagaaga tgcaggaaag acttccaaag
     8101 tcaacttaca aggctttgaa aaagacaatc cagaacggtg aaccgcttga cctcagcgtt
     8161 gcaaatgttg ttgcggctgc tatgaaggac tgggcagttg aaatgggctg tacccactac
     8221 acacactggt tccagcctat gacaggtatt acagccgaaa agcatgacag ctttatcgca
     8281 ccgaacggtg aaggtcaggt tatcatggag ttctcaggta aagagcttgt aaagggcgag
     8341 cctgatgctt cttctttccc aagcggcggt attcgtgcca cattcgaggc aagaggctat
     8401 acaacatggg atccgacatc atatgcattc gttaaggacg gaacacttta cattcctaca
     8461 gcattctgtt cttatacagg cgaggttctc gataagaaaa ctcctcttct tcgttcaatg
     8521 gagagaatta atacagaggc tgtaaagatt cttcatcttc tcggcaaaga aaatgttaca
     8581 agagttacaa caacagtagg tccggagcag gaatacttcc ttattgataa ggacgcttac
     8641 gatcagagag aggaccttat ttatacaggc agaacacttt tcggtgcaaa agctcctaaa
     8701 ggacaggaac ttgacgacca ttacttcggt gcaatcaaga caagagttgc tgcttatatg
     8761 aaagaccttg acgaggaact ctggaaactc ggtatccttg caaagacaaa gcataacgaa
     8821 gttgctcctt cacagcacga gcttgctcct atcttcacaa caacaaacat tgcaaccgac
     8881 cacaacgagc ttacaatgga agttatgaag aaggttgccg aaagacacgg tcttgtttgt
     8941 cttcttcacg aaaagccgtt cgcaggtgtt aacggttccg gtaagcacaa caactggtca
     9001 atttcaacca acacaggcga aaaccttctt gatcccggca agactcctga gaataacctt
     9061 cagttccagc ttttccttgc ggcagttgta aaggctgttc acgagtatca ggatcttctc
     9121 agaatcacgg ttgcatcggc aggtaacgac caccgtctcg gcgctaacga agcaccgcct
     9181 gcaatcattt caatgtatct cggtgatgac ctcggcgaac tcgttgattc aatcatcaat
     9241 gacagagaat atgtaagcaa gggtaagcag aagatgagaa caggcgttga tgttcttcct
     9301 gactttatga aggacacatc ggatcgtaac cgtacatcac cttttgcatt cacaggtaac
     9361 aagtttgaat tcagagcact tggttcttca cttaacattg cctgtcctaa ctatatgctc
     9421 aacactatgg tagccgaaga actttcagag ttctacgatg agcttaaaga tgcggatgat
     9481 atggacgcag ctatcaaggc tcttgttaag aaagtattca cagagcatca gaacattatc
     9541 ttcaacggca acaactacgc tcccgagtgg gttgaagagg ctgaaagaag aggacttctt
     9601 aacctcaagt cacttcctga cgcaatggaa cacttccttg acaagaagaa tattgacctc
     9661 tttgttaaga ataagatttg ttcggcagac gaaatccgtg caagatatga aattgagctt
     9721 gaaagctatt ctaagcagat taacattgag gctctcacaa tgattgatat ggctaagaag
     9781 aacattcttc ctgccgttac atcatatgta agagatctta cggatacagc acttgccaag
     9841 aaggcacttt cagacgctat tcctacaagt gttgaggaag accttattac aagcctttca
     9901 aacaaacttg tatgtttctc aaagaaaact gcagagcttg aagaggctgt tatcaaggca
     9961 agcgactaca gcgatgacaa cctcaagtat gctaagtact acagagaaac agtatttgca
    10021 cttatgcagg aactcagagc tgtcggtgac gcaatggaaa cagaaacagc ttcggaatac
    10081 tggccttatc cttcatacgg cgaacttctt ttcggtgtgt gattgataga acctctattt
    10141 atactaaata acattattta tattgtataa caggtaacac aacggcgtgt gattgacaga
    10201 attaaactgt caactccacg ccgtttttgt tattttgata aaaggagaga tttatatggc
    10261 agaactaatt tacggaggca caggcgtctg gtttatgatc ggcgcattac tcgtgttctt
    10321 tatgcagtgc ggatttgcaa tggttgaaac cggttttaca agagctaaaa acgcaggtaa
    10381 catcattatg aaaaacctga tggacttttg tatcggtacg gttgtattca gcctgttagg
    10441 cttcggactt atgatgtctg aggactatgt tgcaggattc atcggtgttc caaacctcaa
    10501 tatgttcaca gactggatga actttgattg gaactcattc gtattccagc ttgtattctg
    10561 tgctacagca gctactatcg tttcaggtgc tatggcagag cgtacaaaat tctcggcata
    10621 ctgtgtttac agcgcaatta tttcactcgt tgtttacccg attgaagcag gttgggtttg
    10681 gaacggtcag ggttggcttg cacagctcgg ctgtatcgac tttgcaggtt caatcgtaat
    10741 tcatatggtc ggcggtatct cggcacttgt cggcgcaatc attctcggac cccgtatcgg
    10801 taaatacggc aagaacggca aggtaaacgc tatccccggt cacagcatca cactcggtgc
    10861 tctcggtgta ttcattcttt ggtttgcatg gtacggcttt aacggtgccg ctgctgaaga
    10921 cggcgctcgt ctcggtcaga ttcttcttac aacaacaatt gctccggcag ttgcaacagt
    10981 aacaaccatg ctctttacat ggatcaagaa cggcaagcct gatgtttcaa tgtgtcttaa
    11041 cgcatcactc gcaggtcttg tagcaatcac ggcaggttgt gcagatgttg atgcaatcgg
    11101 cgcagcagtt atcggtatcg tttcaggtat cctcatcgta gtagcagttg aatttgttga
    11161 tatcaagctt aaaatagatg atcctgtcgg cgcattctcg gtacacggcg taaacggtat
    11221 gtggggcggc cttgcagtag gtcttttcgc aacaggtaac ggtcagaacg gcatcacagg
    11281 tcttttctac ggcggtggct ttgctcagct tggtaagcag gctctcggta ttgttacaat
    11341 cgtagcttgg acagttgttt gcatgattat cgtattcaca atcatcaaaa agacagtagg
    11401 tcttcgtgtt acaaaggacg aagaaatgaa gggcctcgat atctgcgagc acggtcttat
    11461 cagcgcatat gcagacttta tgccaatcgg tgtcggtaca gcttcacttg atgaaacatt
    11521 tgatgttgac agcaatgttc ctgtaactca ggcagttccc gttcagcttg ccggcaagta
    11581 tgatatggct accgacggca caaagattac taagattgac attcttctca agcagtcaaa
    11641 gcttgaagct cttaaagaag aaatggataa gctcggcatc acaggtatga cagtatctca
    11701 ggtactcggt tgcggtgctc agaagggtaa agcagagtat taccgtggtg ttgctattga
    11761 aagtaacctc cttcctaaga tgaaggttga aatcgtagtt tgcaaggttc ctgtaaaggc
    11821 agttgttgat gcggctgtta aggcacttta cacaggtcac atcggtgacg gcaaaatctt
    11881 tatctatgat gttgaagatg ttgttaaggt tagaacaggc gaaacaggct acgacgctat
    11941 gcaggatatt gaataatccc caatagctta attaacctat cctttttaat tcacataaac
    12001 cgagacgccc cgaacggcaa tagctgttcg gggtgttttg gcatataaat gtttagcaaa
    12061 gataattgac tttgttttgt atatgctata taatgatagt gtaaagaatg atatttgaca
    12121 ctattaaggc ggtggagcgt atgactgatt acagaaagct ttgtatagat ttgttcggaa
    12181 cagataacga aaccgagtta aaggatattg caggcaggct taaaggcgga agaaaaaata
    12241 agttgaccga aacggatgtt aaaaatgcaa ttgaaatgca gaaaaacggt aaaactactg
    12301 ctgaaatagc acaaaccttt aatgtaagcc gtcagacaat ttcaaaatat cttaacaagc
    12361 ccttgaacgg caattatgtt atgcggcttg attttatgtt ccgtcagaag gtgtgtacgg
    12421 aaatatatgt caactttgcc gataagaaaa taaaaattgt gaatcgcacc aatgatataa
    12481 tgaaaagagc tttcggtata aatgaaaatc cggattggaa cgattttaaa cagtttcttg
    12541 aagaaagatg ttttccgaaa tcaagggcat tccgaaaaac tattttgaaa aaaatcggag
    12601 cggacggcta cgatactttg cagatacttg aaaaaacaga cggaagaacg gctgaggata
    12661 accaatatat aaggtttaca agaaaggagc tgtacgcttt ttgattaagg aaatcaatct
    12721 tgacaattac tcacttatag atacaaaatc tgcttcttca aaaggcaatc agcaaaagtg
    12781 gcttgtaaat gatttctggt acaaagcaga ccatatggga tatgagggct tgtgtgaggt
    12841 tattgtatct gaattgttga agaaatcgaa tataaccgat tttgcggaat attttccgat
    12901 aaaaatagaa tttgcaggtc gaaagcttaa cggttgttat tcaaaaaact ttcgccaaaa
    12961 aaatgaaagc attgtcacgc ttgagcattt gtcaaagcaa tggcttgcgt catcaatggc
    13021 gaaaaatctt ttgaagtacg atgatgtgaa ggataaaatt tcccacaccg ttgattttat
    13081 cgagaataaa acaggtttga aaaatgtcgg aaaatatata acagcaatgc ttgagcttga
    13141 cgcatttttt ctgaatgagg acaggcatac caataatatt gcgtttatac tgaatgatga
    13201 tacgggcgaa tacagatttt gtccgtattt tgatttcgga ctttcgctgc ttgccgacac
    13261 aaccgaggat taccctctcg gagaggatat ttacaactta atcccaaaaa ttcacgcaaa
    13321 accttttgat gtagatttcg atgtacagct tgatgctgct caggaactgt atggtgaaca
    13381 ggtaaaattt tcgtttatga gttccgatat agaaaaatct tttgaaaaag tcagcggata
    13441 ttatcccgat gaagttgtgg taagagtgaa aaatttaatc tataatcaaa aacaaaaata
    13501 cggtaatatg tttgagtaat gttcaaatgg aaaattgccc gaaaaatgtt gaaaagtgta
    13561 aaacaaattt gcaatgaaaa tgtttttaca aaattgtaaa aaatattgtc ataattcctt
    13621 taagtaaata acaatagggg ttaatcttgt aaaataataa gatgaatgaa acaatatgcg
    13681 gtggtgaggt tcttgaaaaa tgctttgttg atttcacact ctgaacagag tgccgtttct
    13741 ttgtcacaat tgctcggaag cgaaggctat acggagattt ccgtctgcca aacctcacag
    13801 gaggctaagg agcttgcaaa tggtgaggtt tttgacctca tctgcgtcaa tgcaccgctt
    13861 gaaaaggaaa acggcattga gctttcaaag tactttgccg gaacaacccg ttcaagtgtt
    13921 gtgattatcg ttccgcagcg taatgcggat tatgtgaacg acgcacttac ggagcacggt
    13981 gttcttgtta ttgcaaagcc cgtaaacaag cacctgtttc accacttttt gcagtttacg
    14041 gaatgtttta agatgcgtat gttccgtgtg attgaggaaa atgaaaagct caagcatatg
    14101 gttgcggata tgaagataat aaacagagca aagttcctgc ttattacctg ccttaatatg
    14161 tccgaagatc aggcgcatcg ctatcttgaa aaacaggcta tggatctcag aacaagcaag
    14221 cttgaggttg caaagcaggt tatcagaaca tatgagaact gactgcaatt ttttagttta
    14281 ttgaaaggaa tgtgttttta tgagccgtgc ggcatactta atccttgaaa acggaacagt
    14341 ttttgagggc aaggcgtttg gtgccgaaaa agaaactacg ggagaacttg tttttactac
    14401 agcaatgaca ggttatcttg aaacactcac cgaccccagc tattacggtc aggtggttat
    14461 tcagacattc cctttaatcg gcaactatgg tgttatccct gccgattttg agagtgactc
    14521 tccgtcgctg aaaggttata ttgtacgaga atggtgccaa gttccttcaa acttccgttg
    14581 tgagggtgat cttgacacct ttttaaaaga gtcgggaatt cccggcattt acggaattga
    14641 cacccgtgcg ctcacaagaa ttgtgcgtga atacggtgtt ctgaactgca aaatctccta
    14701 ttctcccgat gtgacaaagg aagaacttga cgagattaag aactatgtta ttacagaggc
    14761 tgttgaaagc actactataa aagaaaaaga gcattttgac gctgaaaacg gcgacctcaa
    14821 tgttgtgctt atggattttg gcgcaaagca taacatcggc agagagcttg ttaagagagg
    14881 ttgtaacctt acagtcgttc ccgcccacac aacagcggat gaaattaagg ctatgaatcc
    14941 cgacggcgta atgctttcaa acggaccggg agatccttcg gacaacacag agattattgc
    15001 agagctcaaa aagctttgtg acttcgggat tccgaccttc ggtatatgtc tcggtcacca
    15061 gctccttgca ctttcacagg gtgcaaaaac agaaaagctc aagtacggtc acagaggtgc
    15121 aaaccagcct gcaaaagagg ttgaaacagg cagagtttac attacatcac agaaccacgg
    15181 ctacgctgtt gtaagcgaca gtatgcccga aaacggcaaa gtaagctttg taaacggtaa
    15241 tgataacacc tgcgagggcg taaactacac aaatatgcct gctttctcgg ttcagttcca
    15301 tccggaagct tgcggcggtc ctcacgatac agcatttttg tttgaccgtt ttattgaatt
    15361 gatgaagaac aacagaaaat aagagtccga aattcggatt actattcagc ataagaacag
    15421 atacaataat tacttgaacg aggaatgatt atatgccact caaaaagaat atcaaacgag
    15481 ttatggtaat cggttcgggc cctatcgtaa tcggtcaggc tgccgaattt gactatgcag
    15541 gcacacaggc ttgccgtgcg cttaaagaag aaggacttga ggttattctc gtaaactcaa
    15601 accctgctac aattatgact gataacgcaa tggctgataa aatctacatt gagccgttga
    15661 cgcttgaaac agttaagaga atcatcaaaa aggaacgccc tgacagcctt ctttcaaccc
    15721 tcggcggtca gacgggtctt accctttcaa tgcagcttgc aaaagagggc ttccttgata
    15781 aatacaatgt tcagcttctc ggtgcaaacc ccgaaacaat cgacaaggca gaggacagac
    15841 agctttttaa ggacacaatg attgaaatag gtcagcctgt tattccgtca cttgttgtta
    15901 cagatgttga atcagctgtt aagtttgccg atgaaatcgg ttatcctgta attatccgtc
    15961 ctgcattcac actcggcggt acaggcggcg gtattgtcga taacgaggaa gagcttcgtg
    16021 aaatcacatc aaacggtctt gaactttctc ctatcacaca ggtgcttgtt gaaaaatgta
    16081 tctcaggttg gaaggaaatc gagtttgagg ttatgcgtga cagcgacgga aatgttatta
    16141 ctgtctgctc aatggaaaac ttcgatcctg taggcgttca cacaggcgac tcaatcgtta
    16201 tcgctcctac ggtaacactt gccgacaagg aatatcagat gctcagaagt gcggctctca
    16261 acattatctc tgcacttaag gtagagggcg gttgtaactg ccagttcgca ctcaaccccg
    16321 aaacatttga atatgcggtt atcgaggtta atccccgtgt atcccgttca tcggcacttg
    16381 cgtcaaaggc aacaggttat cctattgcaa aggttgccgc taaaattgct atcggttaca
    16441 cacttaatga aatcaagaat gccgttacag gtacaacata cgcttgcttt gagccggcgc
    16501 ttgactatgt tgttgtaaaa ttcccgaaat ggcctttcga taaatttgta tatgcaaaga
    16561 gaacactcgg tactcagatg aaggcaacag gcgaggttat ggctattgct ccgacatttg
    16621 agcaggctat tatgaaagcc gtaagaggtg ccgagatttc tcacgatact ctcaacgaca
    16681 aggaatttgc agagctttca aacgcatcgg tttatgacag actcagcgtt tgcgatgaca
    16741 gaagaatctt ctgcgtatat gaggctctca agagaggcat tacagtagaa cagattcaca
    16801 acatcactct cattgacgag tggttccttg aaaagctcaa aaatcttatt gccgttgaaa
    16861 acgaactcaa gaacggcgag cttacggagg acctctatgt tcgtgccaag aacaagggat
    16921 tccttgacag aacaattgaa aaaatcacag gcaggaaggt tgaaaatccg cttgttcctg
    16981 tttataaaat ggttgatacc tgcgccgcag aatttgaggc tgaaactcct tacttctatt
    17041 caacatttga taaggaaaat gaagctgagg aattcatcag agagcagaag agcgataaga
    17101 aaacagttat cgtattcggt tcgggtccta tcagaatcgg tcagggtatc gaatttgact
    17161 atgcatctga tcactgcgta tgggcactca aaaaagcagg ctacgatgtt gttatcgtaa
    17221 acaacaaccc tgaaacagta tcaacagact ttgacacagc cgacagactt tattttgaac
    17281 cgctcacaac cgaagatgtt atgggcataa taaagacaga gaatccgttc ggtgtagttg
    17341 ttgcattcgg cggacagaca gcaattaagc ttacaaagtt cctcagcgac aacggtgtaa
    17401 atattctcgg cacatcatac gatgccattg atatggcaga ggacagagag cgttttgacg
    17461 agcttcttga aaagtatcac atcaagcgtc cccgtggcgt aacggttatg acaacagacg
    17521 aggctctcga tgttgcagac aaaatcggtt atcctgttct tctccgtccg tcatatgttc
    17581 tcggcggtca gaatatgatt attgcattca acgaagatga tgtcaaagag tatatggcaa
    17641 tcattcttgc acagaatatt gaaaatccga ttcttattga taagtacctt cagggtacag
    17701 agcttgaagt tgacgccatt tgtgacggtg aagatattct tattccgggt attatggagc
    17761 atatcgagcg tgccggtgtt cactcgggcg actcaattgc ggtttatccg gcatggaatc
    17821 tctcagacag aatgactcag attattattg aaagctcaaa gaaccttgca atcgaactcc
    17881 gtacaaaggg acttgtaaac attcagtacc ttatctataa agacgaactt tatgttatcg
    17941 aggtaaatcc ccgttcttca agaacaatcc cttacatcag taaggtaaca ggcgttccta
    18001 tggttgacct tgcaacaaga gcaatgctcg gtgaaaagct caaagatatg ggctacggca
    18061 caggtcttta cagaaagagt ccttacattg cggttaaggt tcccgtattc agctttgaaa
    18121 agcttatcaa tgttgataac cacctcggtc ccgagatgaa gtcaacaggt gaagttctcg
    18181 gtgttgcagg cacacttgag gaggctctct ataagggact catcggtgca ggctacaaga
    18241 tgaagaaaaa gggcggtgtg ttcatcacag tccgtaattc ggataaggct gaaatcggtg
    18301 aaatcgcaaa gaaatactac gacctcggat tcagaatcta cgcaacagag ggtacagcgg
    18361 atgtactcaa gaagtatggt atcgatgccg taagcgtaaa gaaaattcac gagtccaaga
    18421 caaacaatac tcttacactc attgaatcgg gcaagattca gtatgttatt tcaacatctg
    18481 caaagggcag aattccttca agagattctg ttaaaattcg tcgtaagact gttgaaagaa
    18541 atattccgtg tcttacatct cttgatacgg caaatgcact tgcagattgc ctcaagagcc
    18601 actattcaca gcacagcacg gagcttatcg acattaacca catgagagag gaaaagctta
    18661 tgttaaaatt cactaagatg cagggtatag gcaatgacta tatctattgc agcacttttg
    18721 atcaggaaat cagcaacccc gaggcgcttg ccgtaagact ttccgacaga catttcggta
    18781 tcggcggcga cggtattatt cttgtttgcc cttcaaaggt tgcagatgca aagatgaaaa
    18841 tgtacaacct tgacggttca gagggtaaga tgtgcggtaa cggtatccgt tgcgtaggta
    18901 agttcctcta tgaccacggt atggttgata tcaacgaaaa agacgaaatt acaattgaaa
    18961 cactcagcgg catcaagaaa ctcaaggctt atacttcagg cggaaaggtt aaccgtctca
    19021 gagttgatat gggcaaggct attcttgaac cgaaggaaat tcctgttgtt cttgacggtg
    19081 acaaggttgt tgacagacct gttgaaattg caggcaagaa ctataacatc acctgcgtat
    19141 caatgggcaa cccacactgc gtggtattca tggacgatat tgataatctt gacattgaaa
    19201 ccgtaggacc ggagtttgaa aacgataagc tcttccctga aagagtgaat acagaatttg
    19261 taacagttct cgatgaccat acaatcaaga tgagagtgtg ggagcgtggc tcaggcgaaa
    19321 catgggcttg cggtacaggc gcttgtgccg ttgcagttgc cgcttgtgaa aacggtttct
    19381 gcaaaaaggg cgaagacatc aagattaagc tcaagggcgg cgatcttatt atcaactata
    19441 ctgatgaaac ggtatatatg acaggtaatg cggaaaaagt atttgaaggc gaagttgagg
    19501 tgtaatcgtg gagataaccg aacataagaa aaataaaaaa tatatatttg taacaggcgg
    19561 tgtcgtatcc ggtcttggca agggtatcac agccgcatct ctcggcagac ttttaaaggc
    19621 aagagggctc aagattgttg ctcaaaagct tgacccgtat atcaatgtcg atccgggtac
    19681 aatgagcccg tatcagcacg gcgaggtata tgtaacggag gacggtgcag aaaccgacct
    19741 cgacctcggc cattatgagc gttttattga tgaggacctt aacaagtatt caaacctcac
    19801 aacaggtaag gtttactcaa atgttcttga aaaggagcgt cacggcgact acctcggtaa
    19861 gacagttcag gtaattcctc acatcacaaa tgaaattaag tcttttatct acaatgtagg
    19921 tgaaaagacc aatgccgatg ttgtaattac ggaaatcggc ggtacggtgg gcgatatcga
    19981 aagccagccg ttccttgagg ctatccgtca ggtatctctt gaagtcggca gagagaacag
    20041 catctttatt catgtaactc tcgttcccta tatcaagggc agtgaggaac acaaatcaaa
    20101 gccgacacag cattctgtaa aagagcttcg ttcactcggt atcaatcccg atatcatcgt
    20161 tcttcgttgt gacgaacctc ttgaggagag cattttcaac aagattgcac ttttctgcaa
    20221 tgtaaagccg gattgtgtaa tcgaaaatat tactctccca acgctttatc aggcacctct
    20281 tatgcttgaa aagagcaatt tctcaaatgt tgtatgtcgt gagctcaata ttgtaacgga
    20341 caaaaagccc gacctttccg agtgggaaga aatgcttagc cgtatcaaca accgcacaaa
    20401 gaaatgcacg attgctcttt gcggtaagta tgttgctctc catgacgcat atctttctgt
    20461 tgcagaggca cttcgtcacg gtggctatga aaattccgcc gatgttgaaa tcaagtgggt
    20521 tgactgcgaa cttcttacaa agtacaaggt tgacgagctt ctcggtgatg ttgacggtat
    20581 tcttgttccg ggcggcttcg gtaacagagg tatcgaaggt aagattatgg ctgcaaaata
    20641 tgcccgtgag catgatattc cgtatctcgg aatctgcctc ggtatgcaga ctgcagtaat
    20701 cgaatacgca agagatgttc tcggctatgc tgatgccaac agcggtgagt ttacaccaga
    20761 gggcaagcat aatgttattg accttatgcc tgaacaggag ggcattgagg atatgggcgg
    20821 cacaatgcgt cttggtgcat atccgtgtgt tatcaagccc gattcgctta tggctaaggc
    20881 ttacggtcag catgaaattt cagagcgtca ccgtcatcgt tatgagttca ataacgattt
    20941 cagagaggaa attgaaaatg caggcatgaa gattacaggt acttcaccta acggacttct
    21001 tgttgaggct gttgaagttc ccgacaaccg tttcttcgtt gctgttcagt atcaccccga
    21061 gttcaagtca cgcccgaacc gtgctcatcc gcttttcaga gaatttatta aggcatcact
    21121 tgaaaagtaa ttgatttttc cacttaactt tcagatttaa aggactgatt ttaatgaaga
    21181 ttaacagaaa ttttgacaat ctcgttccta actatctttt tgcagatgtt gcaaaaaaga
    21241 caaatgcata tattgcggct catcccgata aaaaggttat caagcttggt atcggtgatg
    21301 taacccttcc gcttgctcct gttgttgttg aggcaatgaa aaagggcgca gaggatcttg
    21361 ctcacaagga aaccttcaag ggctatcccg attatgaggg ctacgagttt ctcagacagg
    21421 ctatttcaga ttattacaag agctttggtg ttgcagtagg cgctgacgaa atctttgtat
    21481 cagacggtgc aaagtctgac tgcggtaaca taggcgacat cttctcaaag gataatgttg
    21541 ttctcgtaac agaccctgtt taccccgtat atgttgactc aaacattatg gcaggcagag
    21601 aaattgttta tgccacatca aatgaggcaa acggctttgc cgctctccct gatgaaaatg
    21661 taaaggcaga catcatctac ctttgctcac ctaacaaccc gacaggctct gcatacaacg
    21721 ctgaacagct caagacttgg gttgactatg ctcttaaaaa cgatgcaata atcctctacg
    21781 attcagcgta tgaggcattc atcacagagg atcttcctcg ttcaattttt gcaatcgaag
    21841 gtgcaagaaa gtgcgcaatt gaaatgtgct cactttcaaa gacagcaggc ttcacaggta
    21901 caagatgcgg ttacacggta attcctaagg agcttgagcg tgacggtcac aatatctatg
    21961 caacatggta ccgccgtcag gcaacaaagt tcaacggtgt ttcatggcct gttcagtgtg
    22021 ctgctgccgc tgtattcagc gaagaaggtc agaagcagat caaggagaac atttcttact
    22081 atcaggaaaa tgcaagaatt atcgcatctg ctcttgatga actcggcatc tactacacag
    22141 gcggtaagaa ctcaccttac atctggctca agtgtccgaa caatatgggc agttgggaat
    22201 tctttgacct cctcctcaac gaggcaaatg ttgtaggtac tccgggtgag ggctttggcg
    22261 aaaacggtgc cggctacttc cgtcttacat cattcggtga cagagaaaac acgattgagg
    22321 ctgttgagag aatcaaaaag gttctcacaa acctctcaaa gtaatttgat tgaattcaga
    22381 ttattatatt gaataaaaca cacagcggat aacccgaatt ttgaggttat ccgctgtttt
    22441 tgttatcggt tattatcagt aggggcgctc attgggcgcc cgctaagtaa cggcacaata
    22501 tttgtgataa gacagattat ataaatgcca caaaataatt gtcatactgt agcggcgaac
    22561 aaggttagcc gctactaatt tgtgcc
//
LOCUS       DAOMVE010000012        21748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_409_length_21748_cov_3.090813, whole genome shotgun sequence.
ACCESSION   DAOMVE010000012 DAOMVE010000000
VERSION     DAOMVE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 21748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21748
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_409_length_21748_cov_3.090813"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..184)
                     /locus_tag="PLS64_01445"
     tRNA            complement(100..184)
                     /locus_tag="PLS64_01445"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Leu,seq:gag)
     gene            complement(328..1308)
                     /locus_tag="PLS64_01450"
     CDS             complement(328..1308)
                     /locus_tag="PLS64_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42530.1"
                     /translation="MKKIIKRIVSCVLFVAILLGLLQVSSLIFQPKSNDKAAGIHYPR
                     ANGIFSEPKDSIDTVFIGDSEVYHSFIPLNIWRDYGITSYDVSSPAQKLVYSMEFLKK
                     TFEKQSPKIVFLETNAIFRKSYFEDEITYKAEQIFPVFRYHDRWKNLQLKDFSATVEY
                     TANENNKGYYFTKKSKPATDKAIKKYMKYSDVSAPILSTNKKYLKEIAKFCKKHGTKL
                     VLISTPSTKNWNYQRHNTMEAISKDLGVDYIDTNLLRDDIPIDWKKDSKDKGDHLNYK
                     GAVKLTNYIGKYLDDTKLFKDKRNDPSYDNWNTCLDKFEKQVKKGTSSEN"
     gene            complement(1321..2955)
                     /locus_tag="PLS64_01455"
     CDS             complement(1321..2955)
                     /locus_tag="PLS64_01455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRL42531.1"
                     /translation="MDLPSLLMAGSLDTLTSYFSVAFLAIFLPVSVVVYAVIPQKARK
                     YFLLIASFAFFWLISGQLIAYLCLIIMGVHYFGLWLDRIQTQKSAALKAVPKEEKKAL
                     KKVYLHRSRRVLLFAAVLMIGGLLVIKYSPFFTTNVNSLLLLLNIPIQFDIPSYIMPI
                     GISFFTLQAVSYLFDVYRGLIKADDNILRLALFMSFFPQIVEGPICRYGQTANQLWNV
                     KQIEFENLKLGAVRILFGLMKKLVIADRLNPMIKQIFSHYDDYQGGIIALGAIAYTIQ
                     LYCDFSGTMDAVMGVAQIFGVTMPENFQRPFFSKSISEFWKRWHITLGTWFKDYIFYP
                     VTMSKPMKNLTSSARKKLGNHFGPLLAGSIALFCVWFCNGLWHGSAWGYIFFGMYHFV
                     LILSGNIIAPAVTAFNKKLHINSECFAYKVFQIVRTCILVVIGEMFFRAERLTTGLSM
                     FGKMVTDFSFSSLNYELMKKLKIDMHDVMIVLIALALIFVISVLNEKGICVRGAIAKR
                     NVVIRWAAVYALIMFIIIFGAYGKGYVPVDPIYANF"
     gene            complement(2957..3187)
                     /locus_tag="PLS64_01460"
     CDS             complement(2957..3187)
                     /locus_tag="PLS64_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRL42532.1"
                     /translation="MDELIEILEDIKPDVDYEGCENLIDGHYLDSLSIISLIAEIEDA
                     FDVTVPAVEVVPANFNSVKAMWSMIERLQEEE"
     gene            complement(3189..4367)
                     /locus_tag="PLS64_01465"
     CDS             complement(3189..4367)
                     /locus_tag="PLS64_01465"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRL42533.1"
                     /translation="MNEILKQNKTAFYVFDVKTLKDRVAYLRKMLPEDVAICYAIKAN
                     TFITAELENDVDRFEICSPGEAEICDLLDIPDKMMVISGVYKTPEVMENMVANGKCDR
                     IFTVESLTQFNLFRELSEKYKKKISLLLRLTNGSQFGINSDEIEEIISKRNEFEYLDI
                     LGIQFFSGTQKTSLKKLKREIDKLDNLLILLKEKYDYSAEELEYGTGFPATYFKEDTI
                     NEDELIGGFAQLLNEMTSKPKITLELGRSIAAICGKYYTHIVDKKCNKGENYLLVDGG
                     MNHIVYYGQHMAMKQPYLSVCGKETEPCTESWNICGSLCSMNDIIAKQISLPDIEIGD
                     VICFENTGAYCMTEGISLFLSRDIPSVYIKKEDGTYLCVRDSFETFNLNKPNYRKETN
                     "
     gene            complement(4364..5884)
                     /locus_tag="PLS64_01470"
     CDS             complement(4364..5884)
                     /locus_tag="PLS64_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRL42534.1"
                     /translation="MNSSVLAYLENSAEYFPEKCAVTDEKVSYSYSELVKLSSSVGTA
                     LSELIASGDAVGIYMEKCADAVAAFFATVYAGGFYSVLNTELPQNRLQTTVSVLNPKV
                     IVTSESLLETAKEYFSERKIVTFEDLAKSEPDYAKLGEIRSHKIDTDPLYINFTSGST
                     GTPKGIVVCHRSVIDFIDHFTEIFGIDNNDVIANQAPFDFDVSVKDIYSAVKTGATLV
                     VVPRRMFSAPAELIDFICDRRITVMIWAVSALCLISTFHALDYRTPETVNKILFSGEV
                     MPLKALKNFRSHLPNAKYVNLYGPTEITCNCTYHILDNNCDYADGIPIGKPFPNEDVI
                     LLDENNNRISEVGKVGEICVRGTALALGYYSNPEQNAKAFVQNPLNPYYPELIYRTGD
                     LARYNENGELVFSGRKDFQIKYMGHRIELEEIEREMSAVDGVEQCCCIFDEKKSRLKG
                     FFVGSIEKDELAASMSRDLPAFMVPGVIRRVDEMPLTKNGKIDRKKLAEIAGGRRK"
     gene            complement(6054..7376)
                     /locus_tag="PLS64_01475"
     CDS             complement(6054..7376)
                     /locus_tag="PLS64_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL42535.1"
                     /translation="MKHIKFRILIIVWSSMILLFFAFAGILNIVLPSHFVSEAEDALE
                     YEIDYQHYISNSNDESEDDSSDYYEEDSNEESTLQKKFESGYQDSYFTSDIYYIDVTD
                     ALNDKNTSEGNSLVGSNSTSLKSTYELTEWVKNHNISEEQIYTLQTDNGYYVFTVYEE
                     PQNYDYWDDAESDENDSPVYLMYINIDHIVKYTRSLNWLISAIFLCAIVVMSIIGYRL
                     GVKIEESQKTQRRFFQNSSHELKTPLMAIQGYAEGIEMDVVDPQNAAGIIMQETERMT
                     GLVEEILSISKIDSRHFKLDLVKLDIKEVLYDCFRSLDAVQQMNGISIVPEFPDEEIY
                     VKCDEDQMARAFRNIIINGLKYSKKKITVACETNQKYVYIRFKDDGNGINRDDIEHIF
                     DRFYKGSDGGTGIGLSLANEIIHLHNGSVTAYNYLDGAVFEVKLPIID"
     gene            complement(7373..8080)
                     /locus_tag="PLS64_01480"
     CDS             complement(7373..8080)
                     /locus_tag="PLS64_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL42536.1"
                     /translation="MGASIYIADDEKNIRDLITKFLECDGYSVTAFETGDELMEEFLK
                     NPCELVILDIMMPGTDGLTICRQLRTITDVPIIMLTAKDSEYDYVRGITLGGDDYLTK
                     PFRLTTLLMRVKSLLRRMDMNTKKVIGQELSDIKIGNLRFSAQENCLYCKGKPVSLSK
                     TELKMLTYMLKEPKKAFSREEILEGVWGFDTEVETRVTDETLRRIRRKLSLAGSNVSI
                     GTIWGFGYRISIAEDKK"
     gene            8395..8877
                     /locus_tag="PLS64_01485"
     CDS             8395..8877
                     /locus_tag="PLS64_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HRL42537.1"
                     /translation="MPKTKFQEVIFTIIMVFFMVYAMICYNTALNIGGMKNEIFLSAF
                     KELSFMGPIAFILDFFIIGHIAKKLAFKIVNPAKDNPFFLVLAISAISVIFMCPLMSL
                     AATLIFKNAGVEIISVWLETTALNFPMALCWQLFFAGPIVRFIFRHIFRRSLTADNTH
                     "
     gene            complement(8950..9168)
                     /locus_tag="PLS64_01490"
     CDS             complement(8950..9168)
                     /locus_tag="PLS64_01490"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HRL42538.1"
                     /translation="MKKFIVDRIEGDKAVLECENGDMVNLELKALPKNIKEGDVINFQ
                     ENSYFLNSDETKARKEKIKNLMNSLFED"
     gene            complement(9171..10088)
                     /locus_tag="PLS64_01495"
     CDS             complement(9171..10088)
                     /locus_tag="PLS64_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRL42539.1"
                     /translation="MLKIKINSKLILKLTALFAVICLALCSCSININVNGSDSESKTT
                     VSEIGTLSVHYLDVGQGDSIFIELPNQKCMLIDAGENMYGKSITEYINNLGYTNIDYL
                     VATHPHADHIGSMAYVVKHNNIGEIYMPKVTTTTKTYENLLTAIADKGLKVKSAKAGM
                     NIIDDNDFSINILAPVTIDEDNLNNCSVILKITYKNDSFLFLGDAEKKELETVTADMS
                     AEVLKVGHHGSRTSTTKALLEKVNPKYAVISLGKDNSYGHPHKSTLKLLDEFGVETYR
                     TDENGTVVISTEGSGVTIKTGQTSMKRDE"
     gene            complement(10182..12080)
                     /locus_tag="PLS64_01500"
     CDS             complement(10182..12080)
                     /locus_tag="PLS64_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42540.1"
                     /translation="MPGPMGRRNRGPAQKVENSGQVLKRLFGIIFKKYKFQMIIVFVC
                     ILISVLANVQGSLFIQNLIDDYITPMIQSGSRDFGPMLGAILRVAIFYAIGAASAFIY
                     AKIMVYVTQGTMRDLRCQIFEHMESLPIKYFDTHPHGDIMSVYTNDIDTLRQMISQSI
                     PQLFNSGITIIAVLVSMFTLSIPLTILTLVMVGVMLFVTKQLASRSGRFFAKQQADLG
                     ATNGYIEEMMNGQKVVKVFNHEKKSIEEFNELNDRLFNSSYNANKFANILMPINAQIG
                     NISYVLCAIVGCIFALNNFLGFTIGKLVSFLTFNKSFTQPINQVSQQFNSIVMALAGA
                     DRIFKLLDEEPEVDDGYVTLVNVRKEGDRLVETEKKTGMWAWKHTHSITGETEYRELK
                     GALVMDDVDFGYTDDKIVLHNIDLYAEPGQKIAFVGSTGAGKTTITNLINRFYDIQDG
                     KIRYDGININKIKKGDLRRSLGIVLQDTHLFTDTVMENIRYGRLDATDEEVIAAAKLA
                     NADSFIRKLPDGYNTLLKGDGGNLSQGQRQMLAIARAAVADPPALILDEATSSIDTRT
                     EKMVQDSMDKLMHGRTTFVIAHRLSTIKNSDCIMVLENGRIIERGSHERLLEQKGRYY
                     QLYTGKTA"
     gene            complement(12083..13831)
                     /locus_tag="PLS64_01505"
     CDS             complement(12083..13831)
                     /locus_tag="PLS64_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42541.1"
                     /translation="MIKTLAKNIKGFVKESVLTPFFMILEVIVETIIPFLMSSMIDDG
                     INKGDMQHIFITGGYMGALALTGLFAGMMGGIYGAKASAGFARNLRRSMFANIQSFSF
                     ANIDRYSTAGLVTRMTTDVTNLQNAYQMLLRMCFRAPVSLICAMAMTFIINARLACIY
                     LIATILLSVVLAFIMKATYKYFSQVFEKYDELNASVQENVTSIRVVKAFVREKHEVGK
                     FRKAADNVYRLFVKAENAMVTVSPIMMVTSYASIILVSWFGANMIVSSELTTGELTSM
                     LTYCMNILMNLLMVAMVFVMLTMSLASGKRVAGVLNEKSTLANPENPVYEVKDGSIEF
                     DNVSFSYNTKAEKPILNDINLKIKSGETIGIIGGTGSSKTSLVNLIARLYDVTEGSVK
                     VGGKDVRDYDIETLRDKVSVVLQSNVLFSGTILDNLRWGNENATEEECVNACKLACAD
                     AFIESFPDKYNTKIEQGGTNVSGGQKQRLCIARALLKNPKILILDDSTSAVDTATDAS
                     IRKAFSENIPDTTKLIISQRVSSVSHADKIIVMDDGEISAVGTHEELIKNSPIYNEIY
                     VAQTSGNGDFDAKGEL"
     gene            complement(13849..14331)
                     /locus_tag="PLS64_01510"
     CDS             complement(13849..14331)
                     /locus_tag="PLS64_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRL42542.1"
                     /translation="MCDDKSVKSCKCNKRFNDSIGHEIKVLNQVLQRKLIYSAKDKGV
                     DKLTLMHGWIIVYLYDHLDEDVYQKDIENAFSIARSTVTSILKLMEKKNYITRESVES
                     DARLKKLVLTETGIELHYKIENMITKNETLLDSLLTKEEKKQFLFLINKILSGLENAN
                     "
     assembly_gap    14519..14528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14559..15284)
                     /locus_tag="PLS64_01515"
     CDS             complement(14559..15284)
                     /locus_tag="PLS64_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42543.1"
                     /translation="MKRKFSKKVISILLVFAMLIGGACFSCTSAAAFDLGYISEDGMK
                     LGYSIIKGYSKMSMKKGQYKDGKIAVYCGLDVNNLYVMGSLGWFYMTNGVDLDRAMTD
                     NDYAMEMIAKLDSHLQGAGITNDRAKEKKAEILKDAKYVYQCFDTLGLYIVDVDKNRA
                     DELMNDSGIDFVLDGGCVPKNMKDLNIDGKTDEKDAELIQKYLIKDLTFDDYDENQYA
                     LFVCDINGDKTPDIRDATEILKK"
     gene            complement(15336..16301)
                     /locus_tag="PLS64_01520"
     CDS             complement(15336..16301)
                     /locus_tag="PLS64_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRL42544.1"
                     /translation="MRIKFVDKAIKLYKRITGRFEPITSKCVHDNIFAIAGQSAFFII
                     LSAVPLSMFFVSLLQNLHIPIDFVYHGLSSVFSSKIADDLTYFLTSAYESAVGISLVT
                     LVVTLWSAAQGIHAITNGLNRIYDAYENRNWFFLRIRAMFYTIALFVILLASLVLIVL
                     GSTINDFLSPHLKSLPDIIGIIYHLRYILIFMFLTVLFALIYRNFPNISRSEHKEYGF
                     KSQLPGAFLCTVSWYVLSFGISVYVSDFNGFSVYGGLTRIAVIMIWFYFLMVCLMVCA
                     EINYVYHDMIKAFTVAKFKRKVKTKGSELNQIRKERKNNKSNKSK"
     gene            complement(16332..17099)
                     /locus_tag="PLS64_01525"
     CDS             complement(16332..17099)
                     /locus_tag="PLS64_01525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRL42545.1"
                     /translation="MKALITGASSGIGKEIARYLAELGYDLILVSRSTDKLNKLKEEL
                     SNVNVRVITLDFSRESDSLDLYEHIKNENEQIDFLVNNAGFGAYGKFLDVPLERELEL
                     IHTNVSTVHILTKLFLKDMAERNSGYILNVGSAAGFLAGPTFASYYASKNYVVRLTQA
                     IHEEMRRDNKNVKVSVLCPGPVKTNFNNVADVKFCIRGLDPKFVARYAVDMTMKGKMI
                     ITPGFEMKAVSFFRHFAPEKLLTRISYNVQMKKNGIK"
     gene            complement(17099..17743)
                     /locus_tag="PLS64_01530"
     CDS             complement(17099..17743)
                     /locus_tag="PLS64_01530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL42546.1"
                     /translation="MKYRKLVENAKKPNGFWGKMMIKAMNKGHSSLTSWGLEHMNIER
                     TATVLDIGCGGGKTVDRLCSIVANGKIYGIDYSELSVKSSEKLNSKNILCNKAKILQA
                     SVSDMPFDDNTFDNITAVETYYFWPDKENDVKEVFRVLKQGGTVMLLFEMLRTDDDPF
                     KWEKVENMLNIKSVTPQEIRAVLESAGFENINTYTKEGTTWLCATAQKPKDDEN"
     gene            complement(17794..18444)
                     /locus_tag="PLS64_01535"
     CDS             complement(17794..18444)
                     /locus_tag="PLS64_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42547.1"
                     /translation="MQNTLKIKRTKTALAFNLAIIACEIAAIIIVFFKLGCYPLVYYT
                     EDSNLFALVACAVMAGFQVRYLKSGKEIPHSVQMFKYMAAAVLSVTFIVVLAVLSPMR
                     GEGINGWLYMLFNKDFTFLHTLCPILTILSFVLFEKEPHLNTKSAFTATIPTLIYAIL
                     LIILNIVGAVDGPYAFLRVMNQPLYMSFVWFIVIVGGAFFLALLLQKLNSTGRKRK"
     gene            complement(18464..19828)
                     /gene="rlmD"
                     /locus_tag="PLS64_01540"
     CDS             complement(18464..19828)
                     /gene="rlmD"
                     /locus_tag="PLS64_01540"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HRL42548.1"
                     /translation="MTLKKNDIVNLTITSATAEGSGVGRTDDGIAVFVQGSAIGDELN
                     VRILKVKKTYAFGKIEEILVPSKARITPDCENFMKCGGCTWRHISYEEECKIKQQRVE
                     DAVTRIGGLDNVVFKPIISSDNITRYRNKAQYPVGLDRDGNVVTGFYSFHSHRIINCT
                     DCILQPEIFARVIEITKDFIAKTNTEIYDETTGKGRLRHIYIRIGEVSGELMVCYVVN
                     ANGLKQEDLLVKMLKSELPQLKSVIINSNREKTNVVLGRKNRTIYGSDHITDTLCGLQ
                     FKISPFSFWQINRKQAEKLYGKAKEYANLKSDEILLDLYCGTGTIGLTMADSCKQLIG
                     AEIVEDAVKDANENAKANGIENARFICGDASDAAFQLEKEGLKPDCVILDPPRKGCGE
                     ELVKTISRMSPSRVVYVSCDPATLARDLKFFTENGYTPKEITPCDMFSGTSHVESVAL
                     IERN"
     gene            20147..21514
                     /gene="murC"
                     /locus_tag="PLS64_01545"
     CDS             20147..21514
                     /gene="murC"
                     /locus_tag="PLS64_01545"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRL42549.1"
                     /translation="MDNIKRIHFIGIGGSGMCPLAEILHHEGYELSGSDMNEGETLDR
                     IKGYGIPVFMGHAAENIKGAELVVYSAAIKETNPERQAAKELGIPCIERSVMLGIVTR
                     RYRRSIAVSGTHGKTTTTAMLSQVLIGSGFDPSAIIGGKLPFIGGNSYVGHSDVIVCE
                     ACEYVDTFLQLNPFISIILNIDADHLDYFKNLDNIKKSFNKFAHQTTGLLVINGDDEN
                     TLDAVKDVEIEKITYGFGENCDYRAENISADKGVHEQFDLYFKGEKLTQIKLIVPGKH
                     NIYNALAAAATAHYLGATPKEISENLHKFGGVHRRFEILGTPDGITVADDFAHHPTEL
                     TATLNAAMKMGFNKVWAIFQPHTFSRTAMLLDDFAEALKIPDKAIISAILPVRETNTY
                     NIYSTDLGAKVPGSVCLDTFEEITDYVCKNAKEGDLILTMGGGNVYMCANMIYKQLKY
                     MEENK"
BASE COUNT         6226 a         4533 c         4105 g         6874 t
ORIGIN      
        1 ccacaggact gttttcttaa cggtcgcctt caagtcccgt catcgcttgc ttgcataaaa
       61 tagaaaagac ttgatacgca aggtatcaag tcttttctat ggtgcgagtg acgggacttg
      121 aacccgtacg tcgtgaaaca cacgcccctc aaacgtgcct gtctgcctat tccagcacac
      181 tcgcatttat ctctcacctt tttcgtgagc agtattgatt atacggtaaa actgtaattt
      241 tgtcaatact tttttaaaag ttttttaata tttctttccc ctgcattgaa ttgcaacaca
      301 ggggaaagat tttagattct aaataaatca gttttccgaa cttgtaccct ttttgacctg
      361 cttttcaaac ttatcaagac aggtattcca gttatcgtat gacggatcgt tacgcttatc
      421 tttgaagagc tttgtatcgt caagatactt tccgatataa tttgtcagct tcacagcacc
      481 tttgtagtta aggtgatcgc ctttatcctt tgaatccttt ttccaatcaa tcgggatatc
      541 atcacggaga agattggtat cgatataatc aacaccgaga tcctttgaaa ttgcttccat
      601 agtgttatgt cgctggtagt tccagttttt tgtgcttggg gtacttatca gcacaagttt
      661 tgtaccgtgc tttttacaga atttcgcaat ttctttgaga tactttttgt tggtagaaag
      721 tatcggtgca gaaacatcgg aatacttcat atatttcttg atagccttat ctgtcgcagg
      781 cttagacttt ttagtaaagt aataaccttt gttattctca ttcgcagtat attcaactgt
      841 agccgaaaaa tccttgagct gaagattctt ccaacggtca tgatatctga acacagggaa
      901 aatctgctct gccttatatg tgatttcatc ctcaaaataa gattttctga aaatcgcatt
      961 ggtttcaagg aacacaatct tcggactttg tttttcaaaa gtttttttca aaaattccat
     1021 tgagtacaca agcttttgag ccggagaaga aacatcatac gaggtaattc cgtaatccct
     1081 ccagatattg agcggaatga acgaatgata cacctcactg tcaccgataa atacagtgtc
     1141 aatactgtct ttaggttcgc tgaaaatacc gtttgccctc ggataatgaa tgcccgccgc
     1201 cttgtcattt gactttggct gaaatatgag tgaggatacc tgaagtaatc cgagaagtat
     1261 tgcaacaaac aggacacagc tgacaattct cttaattatt ttcttcataa tcagacctcc
     1321 ttagaagttc gcataaatcg gatcaacggg aacatagccc ttgccgtaag caccgaagat
     1381 tatgatgaac ataataagag cgtaaacagc cgcccatctt ataacaacat ttctctttgc
     1441 aattgcacca cgcacacata ttcccttttc attgagaacg ctgattacaa aaataagagc
     1501 aagcgcaata agcacaatca taacatcatg catatcaatt ttaagctttt tcataagctc
     1561 atagttaagg cttgaaaagc tgaaatcggt caccattttc ccaaacattg aaagacctgt
     1621 tgtaagtctt tctgctctga aaaacatttc gccgataaca acaagtatgc atgtacgaac
     1681 tatctgaaac actttgtacg caaagcattc ggaattgatg tgaagctttt tattgaatgc
     1741 ggtaaccgca ggggcaatga tgttgcccga cagaataagc acaaagtggt acataccgaa
     1801 gaatatgtaa ccccacgcag aaccgtgcca cagtccgttg cagaaccata cacagaacaa
     1861 cgctatgctt cccgcaagca acggaccaaa gtggttgccg agctttttgc gtgcggaaga
     1921 tgtaagattt ttcatcggct ttgacatggt gacgggatag aagatataat ccttgaacca
     1981 tgtgccgagt gtaatatgcc accttttcca aaattctgaa attgattttg agaagaaagg
     2041 tctttgaaag ttttcgggca ttgtaacgcc gaaaatctga gcaacaccca ttacggcatc
     2101 cattgtaccg gagaaatcgc aatagagctg aattgtatag gcaattgctc cgagagcgat
     2161 tattccgccc tgatagtcat cgtaatggct gaaaatctgc ttaatcatag gattaagtct
     2221 gtcagcgatt acgagttttt tcataagacc gaaaagaata cggactgcac cgagttttaa
     2281 gttttcaaac tcaatttgct ttacattcca cagctgattg gctgtctgac cgtaacggca
     2341 gataggacct tctacaatct gcggaaagaa agacataaac aacgcaagtc ttaatatgtt
     2401 gtcatcggct ttaataagtc ctctgtaaac atcaaagagg tacgatacag cctgcaaagt
     2461 aaagaatgaa ataccaatcg gcattatata actcggaata tcaaactgaa tcggaatatt
     2521 caaaagcaat aacagcgaat ttacatttgt tgtaaaaaac ggtgagtatt tgattacaag
     2581 cagaccgcct atcataagta cggcggcaaa caacagaact ctgcgactgc ggtggagata
     2641 taccttttta agagcctttt tctcctcctt aggcacagcc tttagtgccg cacttttttg
     2701 cgtttgaatc ctgtcaagcc acaagccgaa ataatggacg cccattatta tcaggcataa
     2761 ataggcaatc aactgaccgc ttatcagcca aaagaaagca aagcttgcaa taagcagaaa
     2821 atactttctt gctttttgcg gaatgacggc atacacaaca actgaaacag gcagaaaaat
     2881 ggcaagaaac gctaccgaaa aatatgaggt cagcgtgtca agactgcccg ccatcagtaa
     2941 tgatggaaga tccatcttat tcctcctctt ggagtctttc aatcatactc cacattgcct
     3001 ttactgaatt aaagtttgca ggaacaactt ccactgcagg aactgtcaca tcaaaagcgt
     3061 cctcaatctc agcaataaga gaaatgattg aaagagagtc aagatagtga ccgtcaataa
     3121 ggttttcgca gccttcgtaa tctacatccg gtttaatgtc ctctaaaatc tcaataagtt
     3181 catccatttt aattcgtttc ctttctgtaa ttgggtttgt ttaaattaaa tgtttcaaag
     3241 gaatcacgga cgcaaagata tgttccgtcc tcctttttta tgtaaaccga gggaatgtca
     3301 cgacttaaaa acaaagaaat tccctctgtc atacaataag cgcctgtatt ttcaaaacaa
     3361 attacatcac cgatttcaat atcaggcaaa gaaatctgtt ttgcgattat atcattcatt
     3421 gaacaaagcg aaccgcagat attccatgat tcggtacacg gctcggtttc ttttccgcaa
     3481 accgaaaggt acggctgttt cattgccata tgctgaccgt agtacacaat atggttcatt
     3541 ccgccgtcaa caaggaggta gttctcccct ttgttgcact ttttatcaac gatatgcgtg
     3601 tagtatttac cgcagattgc ggcaatgctt cgtccgagct caagcgtgat tttcggcttt
     3661 gaagtcatct cattcaaaag ctgtgcaaat ccgccgataa gctcgtcttc atttatggta
     3721 tcttccttga aataggttgc agggaatcct gtgccgtatt caagctcttc tgcggaataa
     3781 tcatatttct ctttgagaag aataagaaga ttgtcaagtt tgtcaatttc tctcttgagt
     3841 tttttaagag aggttttctg tgttcctgaa aagaactgaa taccgagtat gtcgagatat
     3901 tcaaattcgt ttctttttga aataatttct tcgatttcat cggaattaat tccgaactga
     3961 cttccgtttg taagtcttaa aagcaaagaa attttctttt tatatttttc ggataattct
     4021 ctgaaaagat taaactgtgt aagtgactct acggtgaata ttctgtcgca ttttccgttt
     4081 gcaaccatat tttccataac ttcgggagtt ttgtacacac ccgaaatcac catcattttg
     4141 tcgggaatat caagtaaatc gcagatttca gcctcacccg gtgagcagat ttcaaagcgg
     4201 tcaacatcat tttcaagttc tgccgtaata aatgtgttcg ccttgattgc atagcagatt
     4261 gcaacatctt cgggaagcat ttttctgaga tatgctactc tgtctttgag agtttttaca
     4321 tcaaaaacat agaatgcagt tttgttctgc tttaatattt cgttcatttt cttctgcccc
     4381 ctgcaatttc agcaagcttt ttacggtcaa ttttgccgtt ctttgttagc ggcatttcat
     4441 caaccctgcg gataacgccc ggaaccataa acgcaggcaa atcccttgac attgatgcgg
     4501 caagctcgtc cttttcaatt gagcctacga aaaagccctt gagccttgat tttttctcat
     4561 caaaaataca acagcattgc tcaacaccgt caacggccga catctcacgc tcgatttcct
     4621 caagctctat gcgatgtccc atatatttaa tctgaaaatc ctttcttccg ctgaaaacaa
     4681 gttcaccgtt ttcgttgtat cttgctaggt cgcctgtcct gtaaattaat tcgggatagt
     4741 acggattaag cggattctga acaaaagcct ttgcattctg ttcgggattt gagtagtaac
     4801 cgagtgcaag agcagtacct ctcacgcaga tttcaccgac tttgccaacc tctgaaattc
     4861 tgttattatt ctcatcaaga agaataacat cttcattggg gaacggtttg cctatcggaa
     4921 taccgtctgc atagtcgcaa ttgttgtcga gaatatggta tgtgcagtta caggtgattt
     4981 cagtcgggcc gtaaaggttg acatactttg catttggaag atgtgaacgg aaatttttaa
     5041 gcgccttgag cggcataacc tcaccgctga aaagaatctt attaacggtt tcgggagttc
     5101 tgtagtcaag cgcatggaat gtgctgataa ggcagagagc cgaaacagcc catatcataa
     5161 cagtaatcct gcggtcacat atgaaatcaa tgagttcggc aggagcagag aacattctcc
     5221 tcggcacaac aacaagagtt gcgcctgttt taacagccga atagatatcc ttgacggaca
     5281 catcaaaatc aaacggtgct tgatttgcta ttacatcgtt attatcaatg ccgaatattt
     5341 cggtaaaatg gtcaataaag tcaattacac ttctgtgaca cacaacaatt ccctttggag
     5401 tgcctgttga accggaagtg aagttgatgt agagcggatc tgtgtcaatt ttatggctac
     5461 ggatttcgcc aagctttgca taatcaggct ccgattttgc caaatcttca aaggtcacaa
     5521 tttttctttc agagaaatat tctttcgctg tttcaagcaa gctttcagaa gttacaatca
     5581 cctttggatt gagtacggaa actgtggtct gaagtctgtt ctgcggaagc tccgtgttga
     5641 gaacagaata aaatccgcct gcgtacacgg ttgcaaaaaa tgccgctacc gcatcggcgc
     5701 atttttccat atatatgcct actgcatctc ccgatgcgat aagttcagaa agggcggttc
     5761 cgacagatga gctgagtttt acaagctcgg aatatgaata ggaaaccttt tcgtcagtta
     5821 ccgcacattt ttcgggaaag tattcggctg agttttcaag ataagccagc acactgctgt
     5881 tcatagctgc acctcattat atatctgtat attaattatc aaattataaa ttcgttaacc
     5941 aaatattata tattgcaaac gcatagctat aataccacaa aattcgtcaa aaagcatcac
     6001 aaaaatgtca caagtaagcg attatttgta ttttcagcaa tttgcacaaa taatcagtca
     6061 ataatcggca gtttcacttc aaatacggca ccgtcaaggt agttatacgc tgttacgctg
     6121 ccattgtgaa gatgtataat ctcatttgca agcgacaaac cgatgcccgt tccgccgtca
     6181 ctgcctttgt agaatctgtc gaagatatgt tcaatatcat cacggtttat accgttaccg
     6241 tcatccttga acctgatata aacatatttc tgattggttt cgcaggcaac agtaattttc
     6301 ttttttgaat atttcaaacc gtttattatg atatttcgga atgcccttgc catttgatct
     6361 tcatcacatt tcacatagat ttcttcatcg ggaaattcag gcacaatgct tatgccgttc
     6421 atctgctgaa cagcgtcaag cgatcgaaaa caatcgtaga gaacctcttt tatgtcaagc
     6481 tttacaaggt caagcttaaa atgtcgtgaa tcaatttttg aaattgaaag aatttcctca
     6541 acaagacctg tcattctctc ggtttcctgc ataattatgc ctgcggcatt ttgcggatct
     6601 acaacatcca tttcaatgcc ctcggcatag ccttgaattg ccataagagg ggttttgagt
     6661 tcgtgagaag aattctgaaa gaatctgcgc tgagttttct gtgattcctc gattttaaca
     6721 ccgagtctgt aaccgattat gctcatcaca acaattgcgc aaagaaaaat tgctgaaata
     6781 agccagttga gcgaacgggt atattttacg atatggtcga tatttatgta cattaaatac
     6841 acaggcgaat cgttttcatc ggattctgca tcgtcccaat aatcataatt ctgcggttct
     6901 tcataaacgg taaatacata atagccgtta tctgtttgaa gtgtatatat ttgttcttcg
     6961 gatatattgt gattcttaac ccattcggtg agttcgtatg tactttttaa tgaagttgaa
     7021 tttgagccga caaggctgtt gccctcggag gtatttttat cattaagagc gtctgttaca
     7081 tcaatatagt aaatatccga ggtgaaatac gaatcctgat aaccgctttc aaactttttt
     7141 tgaagagttg attcttcatt ggaatcttct tcataataat ccgaagaatc atcttcactc
     7201 tcatcgttgc tgttagaaat ataatgctga taatcaattt cgtattcaag agcgtcttct
     7261 gcctcactta cgaagtggga cggcaaaact atatttaaaa ttcctgcaaa tgcgaaaaaa
     7321 agtaaaatca ttgacgacca tacaataatc aaaatacgga attttatgtg cttcactttt
     7381 tatcctccgc aatgcttatt ctgtaaccaa agccccatat cgtgcctatg ctgacattac
     7441 tgcctgcaag gcttaacttt ctgcgtattc ttcgcaaggt ttcatctgtt acacgggttt
     7501 caacttcggt atcgaatccc cacacacctt caagaatttc ttctcttgaa aatgcctttt
     7561 tcggttcttt taacatatat gtaagcattt taagttctgt tttgctcaaa gaaacaggct
     7621 tgcctttgca ataaaggcag ttttcctgtg ccgaaaatct aagattgccg attttaatat
     7681 ccgaaagctc ctgacctatt acctttttgg tgttcatatc cattcttctt aaaagggatt
     7741 ttactctcat taaaagggtt gtaagtctga acggctttgt caggtaatca tcgcccccga
     7801 gggttattcc ccttacatag tcatattcgg aatcttttgc ggtcagcata attataggaa
     7861 catccgttat cgttctgagc tgacggcata ttgtcaagcc gtctgttccg ggcatcatta
     7921 tatcaagaat tacaagttca cacgggtttt taaggaattc ctccataagc tcgtcaccgg
     7981 tttcaaatgc ggtaacgctg taaccgtcac actcaagaaa ctttgtaata aggtcacgaa
     8041 tgttcttttc atcatccgca atataaattg atgcacccat agtaaaaata ccctttctga
     8101 acagaaccgc aagttttaag tataattttc atcacaatca tatgtattat accaaatttc
     8161 aaagtaaaaa tccaccgaga aatgaatctg aattgtaata aaagtgtcac aaaatgtatt
     8221 aaacagagta attgcaatga aaattttttt gaagttgaca aaataaaaaa attatagtat
     8281 aatttctgaa atatataatg gtttaattgg aaacgcacat ttctccttaa ggaaatgata
     8341 acgaacaaca cttatatgtt tactaagtta tcatttttat aaaggagatt ttttatgcca
     8401 aaaactaaat ttcaggaagt tatttttacg attatcatgg tgttctttat ggtttatgcc
     8461 atgatttgct acaacacagc tcttaatatc ggcggaatga aaaacgaaat ttttctttcc
     8521 gccttcaaag aactttcttt tatgggaccg attgcattca ttcttgactt tttcattatc
     8581 ggtcacattg ccaaaaagct tgcttttaag attgtaaacc ctgcaaagga taatcctttc
     8641 ttccttgtac tcgcaatttc tgcaatttcg gttatcttta tgtgtcctct tatgagcctt
     8701 gctgccactc ttattttcaa aaatgcgggt gttgagatta tttcggtatg gcttgaaact
     8761 accgcactca acttccctat ggcactttgc tggcagttgt tttttgcagg cccgattgtc
     8821 agattcattt tcagacatat tttcagaagg tcacttacag cagacaacac acattgatta
     8881 cacaatcaca aataaaaatg ctctgtaccg tttgatttgg tacagagcat ttttgttttt
     8941 gcatgatttt taatcttcaa aaagcgaatt catcagattt ttaatttttt cttttctggc
     9001 ttttgtttca tcgctgttca gaaaatagct gttttcctga aagttgatta catcgccttc
     9061 tttgatattt ttcggcaggg ctttaagttc aagattcacc atatcgccgt tttcacactc
     9121 aagaacagcc ttgtcgcctt ctattctgtc aacaataaat tttttcaagc ttattcatcc
     9181 cttttcattg aagtttgtcc cgttttgatt gtcacacctg aaccctcggt tgaaataaca
     9241 actgttccgt tttcatcggt tctgtatgtt tcaactccga attcatcaag caactttaat
     9301 gtgcttttgt gcggatgtcc gtagctgtta tccttgccga gtgaaataac cgcatattta
     9361 ggatttacct tttcaagcaa cgcttttgtt gttgatgttc tgcttccgtg atgacctact
     9421 ttaagaactt ctgcactcat atctgccgta acagtttcaa gctctttctt ttcggcatct
     9481 ccgaggaaca gaaagctatc gtttttgtaa gttattttta gaattacaga gcaattgttc
     9541 agattatcct cgtcaatcgt tacgggagca agaatattta tgctgaaatc gttgtcatca
     9601 ataatattca tacccgcttt ggctgacttg actttaagtc ccttgtctgc aattgccgtg
     9661 agcagattct cataggtctt ggttgtggtt gtaaccttag gcatatagat ttcgccgata
     9721 ttattatgct ttacaacata agccattgaa ccgatatggt cggcatgagg gtgagttgca
     9781 acgagataat caatatttgt gtatccgaga ttattgatat attcggtaat acttttgccg
     9841 tacatatttt cgcctgcatc aatcagcata cacttttgat tcggcagttc aataaatatg
     9901 ctgtcaccct gtccgacatc aaggtaatga accgataatg ttccgatttc tgaaacagtt
     9961 gttttgcttt ccgaatcgga accgttcaca ttgatattga ttgaacacga acaaagtgca
    10021 agacagatta ccgcaaacaa tgcagtaagc tttaatatta attttgaatt aattttaatt
    10081 tttaacacct cacaattctg agtcttattg tacaataaac aagtcaatat ttcaagaaca
    10141 gaaaaaggag agcctttgcg actctcccct ttcgattatc attatgcggt tttgcctgta
    10201 taaagctgat agtatctgcc cttttgttca agaagccgtt cgtgtgaacc acgctcgatg
    10261 attctgccgt tttcaagaac cataatacaa tctgaattct tgattgttga aagtctgtgg
    10321 gcaataacaa aggttgttct gccgtgcata agcttatcca tgctgtcctg aaccatcttt
    10381 tcggtacggg tatcaattga acttgtagcc tcatcaagga taagtgcggg cggatctgca
    10441 acagcggctc ttgcaatcgc aagcatctgg cgctgtccct gactgagatt gccgccgtca
    10501 cctttgagca gggtgttgta gccgtcagga agttttctga taaagctgtc tgcgttggca
    10561 agctttgccg ctgcgataac ctcttcgtct gttgcgtcaa gtctgccgta acggatattt
    10621 tccattacgg tatccgtgaa caggtgtgta tcctgtaaaa ctataccgag tgaacggcga
    10681 aggtcgcctt ttttgatttt gttgatattg attccgtcat aacggatttt accgtcctga
    10741 atatcataga atcggttaat aaggtttgtg attgttgttt tgcctgcacc tgttgaaccg
    10801 acaaacgcaa tcttctgacc gggttctgcg tacaaatcaa tattgtgaag aacgatttta
    10861 tcatctgtat aaccgaagtc aacatcgtcc ataacaagcg cacccttgag ttcacgatac
    10921 tctgtttcgc ctgttatgct gtgagtatgc ttccaagccc acatacctgt ttttttctca
    10981 gtttcaacaa gcctgtcgcc ctctttgcgt acattaacaa gtgttacata tccgtcatca
    11041 acctcaggct cttcgtcaag aagtttaaag atacggtctg cacctgcaag cgccataaca
    11101 attgagttaa actgctggct gacctgattg ataggctggg taaagctctt gttaaatgtg
    11161 aggaagctta cgagtttacc gattgtaaat ccgaggaagt tgttgagagc aaagatacag
    11221 cctacgattg cacaaagtac atagctgatg ttaccgattt gtgcgttaat cggcataagg
    11281 atatttgcaa acttgtttgc gttgtatgaa ctgttgaaaa gcctgtcatt aagctcattg
    11341 aattcttcaa tgctcttttt ctcatggtta aatactttta caaccttctg accgttcatc
    11401 atttcctcaa tgtaaccgtt agtcgcaccg aggtctgcct gctgttttgc aaaaaacctt
    11461 ccgcttcgtg acgcaagctg ttttgttaca aaaagcatta caccaaccat tacaagtgta
    11521 agaattgtaa gaggaatact gagtgtaaac atactgacaa gaacggctat gattgtaatt
    11581 ccgctgttga aaagctgagg aatactctgg ctgatcatct gacggagggt gtcaatatcg
    11641 tttgtgtaaa cactcatgat atcgccgtga gggtgtgtgt caaagtattt tatcggaagg
    11701 ctttccatat gttcaaatat ctgacaacgc aaatcacgca ttgtaccctg tgttacataa
    11761 accataattt tggcgtagat aaacgccgac gctgcaccga tcgcatagaa aattgcaact
    11821 cttaaaattg ctccgagcat cggtccgaag tcacggctgc cgctctgaat catcggagtg
    11881 atgtaatcgt caataagatt ctgaataaac aatgaaccct gaacatttgc aagtacgctg
    11941 atgaggatgc agacgaaaac aataatcatc tgaaatttat attttttgaa gataataccg
    12001 aatagtcttt taagaacctg acctgaattt tcaacctttt gtgcaggtcc tcggtttctt
    12061 cttcccattg gtcccggcat aattacagct ctcctttcgc atcaaaatca ccgttgccgc
    12121 ttgtctgggc aacataaatt tcattgtaaa tcggactgtt tttgataagt tcttcgtgag
    12181 tacccactgc gcttatctca ccgtcatcca ttacaataat cttgtctgcg tgtgaaacgc
    12241 ttgatacacg ctgtgaaata atcagctttg tagtatcggg gatattctcg ctgaatgcct
    12301 tgcggatact tgcgtctgtt gcggtatcaa ctgcacttgt cgaatcatca agaataagaa
    12361 ttttcggatt tttcagcaaa gctcttgcaa tgcaaagacg ctgtttctga ccgcccgaaa
    12421 cattagtacc gccctgttcg atttttgtgt tgtacttatc ggggaagctt tcgataaacg
    12481 catctgcaca cgccagcttg caggcattga cgcattcttc ctctgtagca ttttcgttgc
    12541 cccatctgag gttgtcaagg attgtaccgc tgaaaagaac attcgactgg agcacaaccg
    12601 aaaccttgtc acgcagagtt tcgatatcat agtcacgaac atcttttcca ccgaccttaa
    12661 cagaaccttc ggttacatca taaagtcttg caataaggtt tacaaggctt gtttttgaag
    12721 aacccgtacc gccgataata ccgattgttt cgcctgattt gatttttagg ttgatatcgt
    12781 tgagaatcgg tttttcggct tttgtgttgt atgagaatga aacattgtca aactcaatgc
    12841 ttccgtcctt aacctcgtaa acagggtttt cgggatttgc aagcgttgat ttttcattaa
    12901 gcacacctgc aacacgcttg ccgcttgcaa ggctcattgt gagcattaca aacaccattg
    12961 ccaccataag aagattcata agaatgttca tacaatatgt gagcatactt gtaagttcac
    13021 ctgttgtaag ctcactgctt acgatcatat ttgcgccaaa ccagcttaca aggatgattg
    13081 atgcgtatga agtaaccatc ataatcggtg atactgttac catggcattt tcagccttaa
    13141 caaagagtct gtaaacattg tcggctgcct tgcggaactt gccaacttcg tgtttttcac
    13201 gaacgaaagc ctttacaact ctgattgaag ttacattttc ctgaacgctt gcgttgagtt
    13261 catcgtactt ttcaaaaacc tgactgaaat acttgtatgt tgccttcata ataaaggcaa
    13321 gaacaacaga aagtaaaatt gttgcaatga gataaataca ggcaagcctt gcattgatga
    13381 tgaatgtcat tgccattgca cagatgagcg atacaggcgc tctgaaacac attcttaaaa
    13441 gcatctgata tgcattttga agatttgtta catccgttgt catacgggta acaagtcctg
    13501 cggttgagta acggtcaatg ttggcaaacg agaatgactg gatatttgca aacattgagc
    13561 gtctgaggtt tcttgcaaag cctgccgaag cttttgcacc gtagataccg cccatcatac
    13621 ctgcaaacaa gcccgtgagt gcaagtgcac ccatataacc gcccgttatg aatatgtgct
    13681 gcatatcgcc cttgttgata ccgtcgtcaa tcattgatga cataagaaac ggaataattg
    13741 tttcaacaat tacttcgagt atcataaaga acggtgtaag aacagactct ttgacgaaac
    13801 ctttgatatt ttttgccaaa gtttttatca tacttatctc cttccgtttt aatttgcatt
    13861 ttcaagtccg ctgagaattt tatttataag aaaaagaaat tgtttctttt cttcttttgt
    13921 cagcagactg tcaagcaaag tttcgttttt ggttatcata ttttcgattt tgtaatgaag
    13981 ttcaatacct gtttcggtca agacaagttt tttgagtctt gcatcacttt caacgctctc
    14041 cctcgttatg taatttttct tttccataag tttaagaata cttgtaacgg ttgaacgggc
    14101 aattgaaaat gcgttttcaa tatctttttg ataaacatct tcatcaagat gatcatagag
    14161 atatacaata atccagccgt gcataagagt cagcttatcc actcccttat ccttggcgga
    14221 atagattaac tttctctgca aaacctgatt aagcaccttg atttcgtgac cgattgaatc
    14281 attaaagcgt ttgttgcact tacagctttt tacgcttttg tcatcacaca ttatttcacc
    14341 tccgaaatca cacttatgag atattgttct atatagaata gttcgataca aaacaatttg
    14401 tatcagtaat tatagcacct tcattttgaa tgtcaagaga aattacaaat aattattcaa
    14461 agtttcacat tgctgtcaca aatcaaaagc gacagctaaa attacagctg tcgcagttnn
    14521 nnnnnnnnat attcactttg cacaaattat gacaattgtc acttttttag gatttctgtc
    14581 gcatcacgga tatcgggggt tttatcaccg ttgatatcgc aaacaaaaag agcatactga
    14641 ttttcgtcat aatcgtcaaa ggtcaggtct ttgataagat acttctgaat aagctcagcg
    14701 tccttttcat ctgtttttcc gtcaatgttg aggtctttca tatttttggg aacgcaaccg
    14761 ccgtcaagca caaaatcaat accgctgtcg ttcatgagtt catcggctct gtttttgtca
    14821 acatcaacaa tgtaaagtcc caatgtgtca aagcattgat aaacatactt tgcatccttt
    14881 aaaatctcgg ctttcttttc ttttgccctg tcatttgtta tgcctgcacc ttgaagatgc
    14941 gaatcaagct ttgcaatcat ctccattgcg taatcgttat ccgtcattgc acggtcgaga
    15001 tcaacaccgt ttgtcatata gaaccaaccg agcgaaccca taacatatag attattcaca
    15061 tcaagtccgc agtaaacggc aattttcccg tccttgtact gacctttttt catcgacatt
    15121 ttggaatagc ccttgattat actgtaaccg agcttcattc cgtcctcgct gatatagcca
    15181 aggtcaaacg ccgctgcact tgtgcatgaa aagcacgctc cgccgatgag cattgcaaat
    15241 acgagcaata ttgaaatcac ctttttagaa aattttcttt tcataaaaaa cacctccatt
    15301 ggtagtatca ccgaacggaa gtgttttatt ttgatttatt tacttttatt tgacttatta
    15361 ttttttcttt cttttcttat ttgattaagc tctgaacctt tggtttttac ctttctttta
    15421 aattttgcaa cggtaaacgc ttttatcatg tcatgataga cataattgat ttccgcacaa
    15481 accataagac aaaccataag aaagtaaaac caaatcataa taacggcaat tcttgtaaga
    15541 cctccgtaca cggaaaatcc gttgaaatcc gacacataaa ccgaaatacc gaacgaaagc
    15601 acataccacg aaaccgtgca gagaaacgca ccgggcaact gtgacttaaa gccgtattcc
    15661 ttatgctcgc tgcgtgagat gttcggaaag ttcctgtaaa tcagcgcaaa caataccgtg
    15721 agaaacataa agataagtat atatctgaga tgatagatta ttccgattat gtcaggcaat
    15781 gattttagat gcggtgaaag aaaatcgtta attgttgaac cgaggacaat aagtacaagt
    15841 gacgcaagca aaattacaaa caatgcaatt gtgtagaaca ttgctcttat tcttaaaaag
    15901 aaccagtttc tgttttcgta agcgtcataa attctgttga gtccgtttgt gattgcgtgg
    15961 attccctgtg ccgccgacca cagagtgact acaagcgtta caagagaaat tcccacagca
    16021 ctttcatagg cagaggtcag aaagtatgtt aaatcatcgg caatttttga cgaaaacaca
    16081 ctactgagtc cgtgataaac aaaatcaatc ggaatgtgca ggttttgcaa aagtgacaca
    16141 aagaacattg agagcggcac agccgataat atgataaaga atgccgactg tccggcaatt
    16201 gcaaaaatat tatcgtgaac gcatttgctt gttatcggtt caaatctgcc tgttattctt
    16261 ttgtaaagtt ttatcgcctt gtcaacgaat tttatcctca atgtgtcacc tcccctattt
    16321 ctatatgata attattttat accgtttttc ttcatctgca cattataaga aattcttgtg
    16381 agaagttttt cgggtgcaaa atgtctgaaa aacgacaccg ctttcatttc aaagccgggt
    16441 gtgataatca ttttgccctt catggtcatg tcaacggcat accttgcgac aaacttcggg
    16501 tcaagtcctc ttatgcagaa ttttacatcc gcaacattat tgaagttggt ttttacggga
    16561 cccgggcaaa gtacggaaac cttgacattt ttattatccc tacgcatttc ctcatgaatc
    16621 gcctgagtaa gacggacaac ataattcttt gatgcatagt agcttgcaaa tgtcggtcct
    16681 gcaagaaatc ctgcggcaga acctacattg agaatatatc cgctgtttct ctccgccata
    16741 tctttaagaa aaagctttgt gagaatgtga accgttgaaa catttgtgtg aatgagttca
    16801 agctctcttt caagcggaac atccaaaaat tttccgtatg ctccaaagcc tgcgttgttg
    16861 acaagaaaat caatctgttc attttcattc ttaatatgct catacaaatc caggctgtca
    16921 ctttctcgtg aaaaatcaag cgtaattacc ctgacattca catttgaaag ttcctctttc
    16981 agcttgttaa gcttatctgt tgagcgtgat acaagaataa ggtcataacc gagttctgca
    17041 agatatcggg caatttcttt tcctatgccc gaacttgcgc ctgtaatcaa agccttcatt
    17101 agttttcatc atccttcggt ttttgtgctg tggcacaaag ccatgttgta ccttccttag
    17161 tgtatgtgtt tatgttttca aagcctgccg attcaagtac ggctctgatt tcctgcggag
    17221 ttaccgactt gatattcagc atattttcaa ccttttccca cttgaacgga tcatcgtctg
    17281 ttctgagcat ttcaaaaagc agcataaccg ttccgccctg tttgagaaca cggaaaactt
    17341 cttttacatc attttcctta tcaggccaga agtagtaggt ttcaactgcc gtgatattat
    17401 cgaatgtatt gtcatcaaaa ggcatatcgg atacggatgc ctgaagaatt ttcgctttgt
    17461 tgcagagtat attttttgaa ttgagttttt cggaggattt taccgagagt tcggaatagt
    17521 cgattccgta aatcttgccg ttggcaacaa ttgagcaaag tcggtcaacg gtttttccgc
    17581 cgccgcaacc tatgtcaagt acagttgcgg tgcgttcaat attcatatgt tcaagtcccc
    17641 atgaggtaag gcttgaatgt cctttattca tagccttaat catcattttg ccccaaaagc
    17701 cgtttggctt tttagcattc tccaccaact tgcggtattt cataaaaatc actccaatct
    17761 gaaattttca tctgtttact gtcttattat ctattatttt ctttttcttc ctgttgaatt
    17821 aagtttttga agcagaagtg caaggaaaaa tgcaccgcct actataacga tgaaccaaac
    17881 aaatgacata tacaacggtt gattcatcac acgcaaaaac gcatatggac cgtcaactgc
    17941 accgactatg ttaagtataa taagcagaat cgcatatatt aatgtaggga ttgtagccgt
    18001 gaaagctgat tttgtgttaa ggtgaggttc tttttcaaaa agcacaaacg ataaaattgt
    18061 aagaatcgga cacaaagtat gtaaaaaggt gaagtctttg ttaaaaagca tatagagcca
    18121 accgtttatg ccctctccac gcatcggaga aagtacagca agaactacga taaatgttac
    18181 cgaaagcaca gccgccgcca tatatttgaa catttgaacc gagtgcggaa tttcttttcc
    18241 gcttttgaga tatcgcacct gaaagcctgc cataaccgca caggcaacaa gtgcaaacag
    18301 gttgctgtcc tcagtgtaat atacaagcgg atagcagcct aatttgaaaa acacgataat
    18361 tattgcggca atctcacaag cgataatcgc aagattaaat gccaaagctg tttttgttcg
    18421 ttttatttta agtgtatttt gcattttaat tccttttcta ttgttagttt ctttcaatca
    18481 atgccacgct ctctacatgg gaggttccgg agaacatatc gcagggggtg atttctttgg
    18541 gggtgtagcc gttttctgtg aagaatttta aatctcttgc gagggttgcg gggtcacagg
    18601 atacatagac tacacggctc ggcgacattc ttgaaattgt ctttacaagc tcttcgccgc
    18661 agccttttct cggtgggtca aggattacgc agtcgggttt taaaccctct ttttcaagct
    18721 gaaatgcggc gtctgaggca tctccgcaga tgaatcgtgc attttcaatg ccgtttgctt
    18781 ttgcattttc atttgcatct ttcacggcat cttcaacaat ctccgcaccg ataagctgtt
    18841 tacaggaatc agccattgta agacctattg tacccgttcc gcagtataaa tcaagcagaa
    18901 tttcgtcaga tttaagattt gcatattctt tggctttgcc gtaaagcttt tcagcctgtt
    18961 ttctgttgat ctgccagaac gaaaacggtg aaatcttaaa ctgcaatccg caaagtgtgt
    19021 cggtaatatg gtcacttcca tagattgttc tgttttttct gccgagaaca acatttgttt
    19081 tttctctgtt agaattaata atcacacttt ttaactgcgg aagttcggat ttgagcattt
    19141 tcacgagtaa atcttcctgt ttaagaccgt ttgcgttgac aacatagcac accataagct
    19201 cgcccgaaac ctcgccgatt ctgatataaa tgtggcgaag tctgcccttg cctgttgttt
    19261 catcataaat ttccgtgtta gtttttgcga taaaatcttt tgtgatttca attacccttg
    19321 caaaaatttc gggctgtaaa atacagtcgg tgcaattgat tatgcggtgg ctgtggaatg
    19381 aataaaagcc cgtcacaaca tttccgtcac ggtcaagacc gacaggatac tgtgccttgt
    19441 tgcggtatct tgtgatatta tccgacgaaa ttatcggctt gaaaacaaca ttatcaagac
    19501 cgccgatgcg tgttacggca tcctcaactc tttgctgttt tattttgcat tcttcttcat
    19561 aagaaatatg tcgccatgtg caaccgccgc acttcataaa gttttcgcag tcgggagtaa
    19621 ttctcgcctt ggacggaacg agaatttctt cgattttgcc gaaagcgtag gttttcttaa
    19681 cctttaatat acgcacattg agttcgtcac cgattgcaga accctgtaca aaaacggcaa
    19741 tgccgtcatc tgttcttccg actccgctgc cctcggcagt tgccgaagtt attgtaaggt
    19801 ttacaatatc gttctttttt aaagtcattt tatcactctg ccattttatg tgtattttta
    19861 gttataatcc tattcccctt tattttagca tttactctac aatttggcaa caattaaaac
    19921 acagtatccg tacaatacac aaaaattgca caaatatcaa attaggtatt tatttttttg
    19981 tccattcgtc ttataatatg agtattgcag ttctgtcacg gcattgctct ttgaatgcaa
    20041 atcaacctgt gttccgactt aaatacaggc aaagcggaat gatttttctg acagacaaat
    20101 atacttatca ggaatggagt ggactattgt gaattacgac agtcttatgg ataacataaa
    20161 aagaatacat tttatcggta taggcggcag cggaatgtgt ccgctggctg agattcttca
    20221 ccacgagggc tatgagcttt cgggttcgga tatgaacgag ggtgaaactc ttgacagaat
    20281 taagggttac ggcattcccg tatttatggg acacgccgca gaaaatatta agggtgcaga
    20341 gcttgttgtt tactcagcgg ctattaagga aactaacccc gaaagacagg cggcaaagga
    20401 actcggtatt ccgtgcattg aaagaagcgt aatgctcggt attgttacaa gaagatacag
    20461 aagaagtatt gcggtttcgg gtacacacgg caaaacaact acaacagcta tgctttcaca
    20521 ggtgcttatc ggcagcggtt ttgatccgtc agctattatc ggaggaaaac ttccgttcat
    20581 cggcggtaac agctatgtcg gtcacagcga tgtaatcgtt tgtgaggctt gtgagtatgt
    20641 tgacacattc ttacagctca atccgtttat ttcgattatt cttaatattg acgcagatca
    20701 tcttgactac ttcaagaatc ttgacaacat taagaagtca ttcaacaaat ttgctcatca
    20761 gacaacaggc ttgcttgtta tcaacggtga cgacgaaaac actcttgatg ccgtaaagga
    20821 tgttgagatt gagaaaatca cctacggttt tggcgaaaac tgcgattaca gagctgaaaa
    20881 catcagcgca gacaagggtg ttcacgagca gtttgacctt tacttcaagg gtgagaagct
    20941 cacacagatt aagcttatcg ttcccggaaa gcacaacatt tataatgcac ttgccgctgc
    21001 ggctactgca cattatcttg gcgctactcc gaaggaaatt tccgagaatc ttcacaagtt
    21061 cggcggtgtt cacagaagat ttgaaattct cggcacacct gacggaatta ctgttgccga
    21121 tgactttgct catcatccca cagaacttac cgcaacactc aatgccgcaa tgaaaatggg
    21181 ctttaacaag gtttgggcaa tattccagcc gcatacattt tcaagaacag ccatgctcct
    21241 tgacgatttt gcggaggctc ttaaaattcc ggataaggca attatttcgg ctattctccc
    21301 tgttcgtgaa acaaacacat ataacatcta ttctactgac ctcggtgcaa aagttccggg
    21361 ttctgtatgt cttgacacat ttgaagaaat taccgactat gtttgcaaga atgcaaaaga
    21421 gggcgacctc attctcacaa tgggcggcgg taatgtgtat atgtgtgcca atatgattta
    21481 caaacagctt aaatatatgg aagaaaataa ataagatttt taaacaaaca gaagaaccgt
    21541 gccataaaag cacggttctt ttttctttta ttgaatattt aagcaaaaat gaacaaagct
    21601 ccgtgcggtt acggagcttt gttcgttaag agagagtttt tgtctacagc tttggctgta
    21661 tgaatattta tataaaaagc taaaaagctt gttggctgtt attgaccgaa catatagttc
    21721 cagattgagt cgttgtcact gttatctg
//
LOCUS       DAOMVE010000013        20188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_473_length_20188_cov_3.310982, whole genome shotgun sequence.
ACCESSION   DAOMVE010000013 DAOMVE010000000
VERSION     DAOMVE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_473_length_20188_cov_3.310982"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..787)
                     /locus_tag="PLS64_01550"
     CDS             complement(35..787)
                     /locus_tag="PLS64_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HRL42550.1"
                     /translation="MLNQFSRTQLLIGKEAMDRLSKTRVAIFGIGGVGGYTAEALARS
                     GVGELDLIDDDKVCLTNINRQIFATRKTVGKYKVDVAEERLKDINPDIIINKHQTFYT
                     PETSEKFDFSKYDYVVDAIDTVTGKIELVMQADKAKTPIICSMGAGNKLDPTAFEVAD
                     IYKTSVCPLARVMRHELKKRGIKKLKVVYSKEKPITPVDDMAISCKTNCICPPETERK
                     CTQRRQVPGSTAFVPSVAGLIIAGEVIKDLIK"
     gene            1110..2249
                     /gene="hemA"
                     /locus_tag="PLS64_01555"
     CDS             1110..2249
                     /gene="hemA"
                     /locus_tag="PLS64_01555"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRL42551.1"
                     /translation="MYCLSVSHKTSNVVVRKKLAFPDEQKKTFLDDLYYSENISECLI
                     LCTCNRTEVYFCGDESSVKTVETVLSDFSGIDFDELKKYICLFYGDRALLHLFRVAGG
                     IESMVIGEDEILGQLKRAYAFAKDNGTVAYELNMCVQAAVACAKKIKTETAISKTSVS
                     VATLASNEAAKFKDGELNVLVIGATGKVGSTVVKNLLSHKGISVTVTSRKHKAETVFE
                     NTGANVIDYDERYKAFDFADCVISATSGPHYTVTYYDLKNNIKTEKSRLFIDLAVPPD
                     IDENIPKISGVRLINIDCIKQLANENNALKLDSVESAKEIIFEEIEVLKKDLAFHEFL
                     PCMQSVKNAMPSDFSEKFIYKLKSDVSAEEFARILEIYRKCGENN"
     gene            2249..2857
                     /locus_tag="PLS64_01560"
     CDS             2249..2857
                     /locus_tag="PLS64_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HRL42552.1"
                     /translation="MAYFPFYIDIENKKILVVGGGTVALRKIEKLTPFSPDITVVAPK
                     ICDEIKALNVKIIDRRFCDSDLDGAFCVVSATDDETLNGRIFQLCNEKNILVNTVDDK
                     EKCGFIFPAIASKNGITAGITTSGKSPIYAKYLKELFVGILESMNENTTEVLWKYRPI
                     IKEKVEKEDDRRKIFEQLLSLCLSGLEPDEKTVENLIEEYEQ"
     gene            2854..3714
                     /gene="hemC"
                     /locus_tag="PLS64_01565"
     CDS             2854..3714
                     /gene="hemC"
                     /locus_tag="PLS64_01565"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRL42553.1"
                     /translation="MTIKIGTRKSRLALAQTEMLIKTLKNRFPNVKIETVYISTTGDK
                     TLDKPLAALGGKGVFIKELEMSLLSGETDVAVHSAKDLPTKIADGLEISAVLPRGSYG
                     DVFVTRNNFETKDGFVIGTGSSRRKLFAEKIYPNAKFKDIRGNVDTRLKKLLNGEYDA
                     LVLAKAGLERLDLCNGEDYTVTPFDADEFLPAPCQGIVAIESRKNSEVSKMLAEISDK
                     NTMLSFETERQVLHSLNADCSTPVGAISKVENDRITLSLSADCKKTVSGTDEISNRIK
                     LAKRLVSEIE"
     gene            3707..5191
                     /gene="cobA"
                     /locus_tag="PLS64_01570"
     CDS             3707..5191
                     /gene="cobA"
                     /locus_tag="PLS64_01570"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRL42554.1"
                     /translation="MSNGRVYIVGAGCGDFDLITMRGAEYIRHCDVLVYDSLIDVSLL
                     GFAPETAEKICVGKRSGRHSEKQENINEILVEKAREGKTVVRLKGGDPFVFGRGGEEI
                     ETLKSADIPFDIVPGISSSIAVPELAGIPVTHRNLSRSVHIITGHTAQDTLPENIKKC
                     AETDGTLVFLMGLRNLPDIVENLIRNGKSEDTPVAVVSNGACAKNQTVRGTLSTICEN
                     VKSAEVVPPAVIVVGETAKFDFAPTITRPLKNVSVTVTGTRKLSDKLGKMLTLSGAEV
                     KRHDLLKVIEYHDNEAFDNAINGIDGYDYVVLTSMNGAEIFMSRLRKLKKDIRSFANV
                     KFAVIGSGTAAVLEKYGIFADCIPNVYTTRELGILLAKTVKSGEKVLILRAENGSPEL
                     TRILADNNIDFDDVKTYDIQLEKKQDNSVVDTDFITFASASGVNTFFDGGAIISDRTS
                     VVCIGEITAEALESHGVNSFKIAKTKDTDGILKTIIKEVTLKEG"
     gene            5196..6167
                     /gene="hemB"
                     /locus_tag="PLS64_01575"
     CDS             5196..6167
                     /gene="hemB"
                     /locus_tag="PLS64_01575"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRL42555.1"
                     /translation="MRRFRRLRAGEQIRSLVRETRIDKGDLIYPIFIAEGENIKKPVD
                     SMPNVYQYSLDRADEILKEIENSGIAGLLIFGIPKHKDELATSAYDDNGVTQNAIRYI
                     KKNYPSLLIIADVCLCEYTSHGHCGVVCGHEILNDETLPLLSKMAVSLAKAGADIIAP
                     SDMMDGRVSAIRNALDENGLINTPIMSYSAKFASGYYSPFRDAAESAPEFGDRKSYQM
                     DYANGKEALREIADDIDEGADMVMVKPALAYLDIVKAASERFDLPLVAYNVSGEYAMV
                     KAAAEKGWIDEKKIVCENMIAIKRAGADIIITYHALDVAKWIDEFYK"
     gene            6180..7442
                     /gene="hemL"
                     /locus_tag="PLS64_01580"
     CDS             6180..7442
                     /gene="hemL"
                     /locus_tag="PLS64_01580"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRL42556.1"
                     /translation="MNTTQSEKLYAQALDFMPGGVNSPVRACRSVGRTPLFIDRAEGS
                     KIYDVDGNEFIDYICSWGPNILGHKQPDVIKAVTSACQNGLTFGACHSGEIELAELIR
                     KNMPSIEMLRLVNSGTEAVMSAIRAARGFTKRDKIVKFEGCYHGHSDGLLVKGGSGLL
                     TNSIPNSAGVPKGYTDTTLLAKYNDEESVQQLFDNCGDEIACVIVEPCAANMGVVPPK
                     KGFLKFLREITEKHDSLLIFDEVITGFRLSLGGAQKLYGVKPDLTTLGKIVGGGMPLA
                     VYGGRKEIMECVAPLGSVYQAGTLSGNPVAVSAGIATLKILEKNKDSIYPELERKSAK
                     IENAMKNAGLNVNRVGSIMTAFFTDKKVVDYDTATTADTKRYAEYYNHLLENGIYAAP
                     SQFEAMFVSFAHTDEDIEKTCQVIESFK"
     gene            complement(7505..7957)
                     /locus_tag="PLS64_01585"
     CDS             complement(7505..7957)
                     /locus_tag="PLS64_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRL42557.1"
                     /translation="MKISTKVEFGIIAVIDIASHSLNGNAVTTPEIAKRQNISKKYLE
                     QILSSLSSAGLVRGQKGSNGGYVIAKNLKEITFKDIINALDANVLSNVYFNTRGDDDV
                     MIRLIDNSLWSKMSSYLQEYAESITLAQLCDEYIKNNEIEHNIPMYYI"
     gene            8250..9497
                     /locus_tag="PLS64_01590"
     CDS             8250..9497
                     /locus_tag="PLS64_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRL42558.1"
                     /translation="MEFNTALLHKGFNGEKVFGSTLTPIYQVSAFSHSSAEQLEKVFN
                     NRAPGYAYSRIGNPTVTSFENRIASIENGVGAVACSSGMAAVTMALLNILESGDEVIA
                     SAGLFGGTIDLFGDLEPFGIVTRYVNKVTAEEIEPLINEKTKAVFAELIGNPGLEIAD
                     LDAVSELVHSHGVPLIIDSTTATPYLIQPFEHGADIVVHSSSKYINGGGNSISGIIVD
                     SGNFEWNTERYKGLAEYKKFGRLAYVAKLRNGIWRNIGCCLAPFNAFMNSVGLETLGL
                     RMERLCYNALELAKFFETQDGIEVNYPALEASPYYSLCQKLLKGKGGAILTIRAGSKE
                     RAFKLINNLKYATNATNIGDTRTLVIHPSSTIYAHGTEEQKRNAGVFEDTIRISVGIE
                     DIEDLKSDFKQAVDSINVNESEE"
     gene            9503..10363
                     /locus_tag="PLS64_01595"
     CDS             9503..10363
                     /locus_tag="PLS64_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRL42559.1"
                     /translation="MAVDYATLKKGGFMRQKQKNNFSLRLRVVGGNLTAKQLAKIAEV
                     SENYGDGYVHLTSRQSVEIPFVKLDDVEAVKDALAEGDVEPGVCGPRVRTITACQGEA
                     ICPSGCIDTYALAKELDDRYFAKELPHKFKFGITGCQNNCLKAEENDVGIKGGIKVSW
                     KEDACIGCGVCVKACRQGAITLEDGKISVDNSKCNYCGRCFKACPTDAWDTIHGYIVS
                     FGGLFGNSINKGETIIPFIENKEKLMEICDAALNFFAENAKPSERFKFTIDRVGREKF
                     EQRILEVYNG"
     gene            10356..10601
                     /locus_tag="PLS64_01600"
     CDS             10356..10601
                     /locus_tag="PLS64_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013977449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HRL42560.1"
                     /translation="MADFVIDDTVDITDVVCPTTFVKAKVALEELDEGQILSIKMNDG
                     EPVQNVPRSIKEEGHKILKLIDNEDGTYDLIVEKVGD"
     gene            10602..10925
                     /gene="trxA"
                     /locus_tag="PLS64_01605"
     CDS             10602..10925
                     /gene="trxA"
                     /locus_tag="PLS64_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRL42561.1"
                     /translation="MPQRVSKDNFQSEVLSADTLVLADFYSDSCIPCKRLSPVLFELE
                     KEYEGKLKIAKINVNFDSELASEYEVQAAPTLVFFKNGEEKERLRGAVKKSEIIEVTE
                     KLIKE"
     gene            10931..11137
                     /gene="thiS"
                     /locus_tag="PLS64_01610"
     CDS             10931..11137
                     /gene="thiS"
                     /locus_tag="PLS64_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRL42562.1"
                     /translation="MTITVAGNKKEVADNLTVAQLIIDENVETPEYVTVTINDEFVKS
                     GTFEETTLKDGDVVEFLYFMGGGQ"
     gene            11137..11949
                     /gene="thiF"
                     /locus_tag="PLS64_01615"
     CDS             11137..11949
                     /gene="thiF"
                     /locus_tag="PLS64_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HRL42563.1"
                     /translation="MAFSNEQLERYSRHIILKEVGAKGQKKLLNAKVLIIGAGGLGAP
                     AAMYLAAAGVGTIGIADADEVDLSNLQRQIIHSTQDIGKPKVESAKETMEAINPDVTV
                     KTYHTFVTSENILDLVKEYDFIIDGTDNFPAKFLINDACVMAKKPFSHAGIIRFKGQL
                     MTYVPGEGPCYRCVFKNPPPKDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYIIGQGEL
                     LTGYLLTYDALKMEFRKVKLPSDTHNCAVCGDHPTITELIDYEQAECDGVHL"
     gene            11946..12356
                     /locus_tag="PLS64_01620"
     CDS             11946..12356
                     /locus_tag="PLS64_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HRL42564.1"
                     /translation="MIKLTKSDFEKILAHAVKELPDEACGLIAGTVEGGDKEIKKVYL
                     LTNIDHSNEHFSLDPKEQLEAIKDMRKNGFVPLGNWHSHPESPSRPSEEDKRLAFDSK
                     ASYMILSLMDRENPVLNSFKITGDTAEKEELVIE"
     gene            12369..13280
                     /gene="trxB"
                     /locus_tag="PLS64_01625"
     CDS             12369..13280
                     /gene="trxB"
                     /locus_tag="PLS64_01625"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRL42565.1"
                     /translation="MYDIVIIGSGPAGLSAAIYAQRENLKAVVVEKEFMGTGQIAESE
                     RVDNYPGLYGETGFDLGEKFRTHAEKLGAEFVEGEVTEVERVNGNFSVNIDGGESLET
                     KTVIYAAGTQRRKLGVKGEKEFSGNGVSYCAVCDAAFYKDKITVVTGGGDTALGDALL
                     LSRFCKKVYLVHRRDSFRAGRALQNRVFATENIEALTNATVSEINGDTKVTSVTLLQN
                     GEEKQLDCNGVFVAVGSVPDTKPLANLVKTDKDGYIIADESGKTSADGIFAGGDIRTK
                     ALRQVVTAVSDGANCVISAEKYLDEKQ"
     gene            13301..14983
                     /locus_tag="PLS64_01630"
     CDS             13301..14983
                     /locus_tag="PLS64_01630"
                     /EC_number="1.8.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate reductase subunit alpha"
                     /protein_id="HRL42566.1"
                     /translation="MKTEKISTDVLIIGGGTAGCYAALTISENSDKKVLICEKAHIKR
                     SGCLAAGVNALNAYIVEGRKPQDYVDYAKKDADGIVREDLLLTMSEKLNEVTDRLEKL
                     GLVILKDENGKYVTRGNRNLKINGENIKPILADAVEKAKNVTVLNRVNIFDYSVKDNK
                     INGAFGFGIESGIFYTIEAKAVIIATGGAAGLYKPNNPGFSRHKMWYPPFNTGAGYAM
                     GIRAGAEMTTFEMRFIALRCKDTIAPTGTLAQGVGAEQINSLGEVYETKYGLTTSERV
                     YGTVNENQEGRGPCYLRTEGITAEQDESLLKAYLNMAPSQTIKWIESGRNPSRQNVEI
                     EGTEPYIVGGHTASGYWVDTDRATTIEGLFAGGDVAGGCPQKYVTGALAEGEIAGLSA
                     VKYIDSKESFEKISDEDTNYHLRETEKYLTDRHSLYTTEQLEEAMQTVMDSYAGGIKT
                     NYRFNEKQLDIADCKIRQLETLTDDLYAEDFQELMYICELKERLTVCKSVIAHLRARK
                     ETRWHSFAENLDYPEKDDRNFNKYVNSRLENGEIKIIIRDLVTGGEKYEHSN"
     gene            14967..15281
                     /locus_tag="PLS64_01635"
     CDS             14967..15281
                     /locus_tag="PLS64_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRL42567.1"
                     /translation="MSIVIDKSKCIGCKKCRNVCPGSLIKTDENGKAYIKYPKDCWGC
                     TSCIKECPAYAINFFLGSDIGGMGSKVHTEKNGDILSWLIEKPHGEVIKIDINQKESN
                     KY"
     gene            15323..16222
                     /gene="cysD"
                     /locus_tag="PLS64_01640"
     CDS             15323..16222
                     /gene="cysD"
                     /locus_tag="PLS64_01640"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRL42568.1"
                     /translation="MSQLSHLDELEAEAIYIIREVAAECEKPVMLYSIGKDSSVMLHL
                     AMKAFYPEKPPFPFLHIDTTWKFKDMIKFRDDTAKKLGIEMLVYTNEEGVKKGINPFD
                     HGAAYTDIMKTQALKQALNKYGFTAAFGGGRRDEEKSRAKERIFSFRNKAQAWDPKNQ
                     RPEMWKLYNTKINKGESIRVFPISNWTEKDIWQYIQREKIDIVPLYFAAKRPVVYRDG
                     NIIMVDDDRFPLKEGEVPELKSVRFRTLGCYPLTGGIESTATTLDEIIDETLSSVSSE
                     RTSRVIDNEAQGSMERRKREGYF"
     gene            16222..17910
                     /locus_tag="PLS64_01645"
     CDS             16222..17910
                     /locus_tag="PLS64_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL42569.1"
                     /translation="MKGLLKFITCGSVDDGKSTLIGHILYDAKLIYADQEKALELDSK
                     VGSRSGDIDYSLLLDGLMAEREQGITIDVAYRYFTTDNRSFIVADTPGHEEYTRNMAV
                     GASFADLAVILIDASQGVLVQTRRHARICKLMGIRHFVFAVNKMDLVKYSKSRFDEIV
                     KQIEELKNELLLDDIYIIPLSATEGDNVTVKSENIPWYNGVPLLQYLETVDVDSSEEE
                     EGFYLPVQRVCRPDHTFRGFQGQIESGSISIGDEIVTLPSNEKAHVKQILMTDKDVKT
                     AFKGQPVTITLDKEVDVSRGCVITKDTNLASYQELTASILWMDDEQLTAGKDYLVKLG
                     TKTISGIVSEIKYAVDVNTGEHIPADSLTKNGIAVCTILLAEPIAVDLFSKHKTLGEL
                     ILIDRVSNMTSACGVVDSVEEKADDAKKASFVLGSLEARGDIFEEFFYDTSSLNVLKY
                     QPVKETYTKGDTIPVEGESYKYPDSFDIIVLRDSVAVKVRDRKITDIVPTSEYSYGGV
                     PVVNGRGFEVLADSNEKIQQFLSEYSNLKSINDADFFAKWVKFDTYRKIAIQNR"
     gene            18080..18280
                     /locus_tag="PLS64_01650"
     CDS             18080..18280
                     /locus_tag="PLS64_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HRL42570.1"
                     /translation="MSKTAAVLITFFLGELGVHRFMTGKIGTGIIWLLTCGVFGIGWL
                     VDFIMVLTGKFTDKNGNVWGAN"
     gene            18298..18681
                     /locus_tag="PLS64_01655"
     CDS             18298..18681
                     /locus_tag="PLS64_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42571.1"
                     /translation="MAFGVKTKNIKTQNSYTIESFFDAIKDKEFTAGTPSLTKHGMAT
                     VITFPALDSQNQVWIMNNGFGKETQKFSIQKSTQAGMDNVAKNAALDSITGGLFSVGG
                     MISENTKKCEKLVDATAKELSEMNL"
     gene            18761..19294
                     /locus_tag="PLS64_01660"
     CDS             18761..19294
                     /locus_tag="PLS64_01660"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRL42572.1"
                     /translation="MFFNDYYNSPIGNITMASDGESLCGLWFDGQKYFASTVKGETED
                     KTLPVFEQTKKWLDIYFSGNEPDFMPKLSLNGSEFRKAVWDILLTIPYGSVMTYGEIA
                     KILAKQRGVAKMSAQAVGGAVGHNPISIIVPCHRVVGTNGNLTGYAGGIDKKISLLKI
                     EKTDVKKLIQKFPKRIN"
     gene            complement(19287..>20188)
                     /locus_tag="PLS64_01665"
     CDS             complement(19287..>20188)
                     /locus_tag="PLS64_01665"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRL42573.1"
                     /translation="VFGMFIILSSPLKIKTKLVVESITKPTIIMILIFLYLGGSMATF
                     GTYYIIPSSDYRTITLKILTLIVSIVFSFSIPILLYNQIKKSRYMYENDIYERQLNAQ
                     INYYKSITTSGYELRKIRHDYNDLSIGLKSLINSRKYDEVLPLLQKYDSEIISSFQIL
                     YNTGNDLTDAILSDKQKNADENIKITFEGSLANVPADNLEICVLFNNMIDIAFENVKE
                     FCGNGKQIIALKAETRAGFLFCCISFPIENEIKKAQNNVLYHSFAYKTLKNLAEKNGG
                     KIETSLSDDRLAITTGWVINNFS"
BASE COUNT         6191 a         4062 c         4654 g         5281 t
ORIGIN      
        1 ttctctcggt gctttgcttt gttaaaaatt atatttactt aatcaaatcc ttgataactt
       61 cgccggctat gataagacct gcaaccgacg gaacaaatgc ggtactgccg ggaacctgtc
      121 tgcgttgggt gcattttctt tcagtctcgg gagggcatat gcaatttgtt ttacagctta
      181 ttgccatatc gtcaacgggt gtgataggct tttcctttga ataaacaacc ttcagctttt
      241 tgatacctct ctttttgagt tcgtgtctca taacccttgc aagaggacag accgaggttt
      301 tgtaaatgtc ggcaacctca aacgctgtag gatcaagctt gttgcctgct cccattgaac
      361 agatgatagg cgtcttggct ttgtcggctt gcataacaag ctcaattttg cctgttaccg
      421 tgtcaattgc gtcaactaca tagtcgtatt ttgaaaagtc gaatttttcc gaggtttcgg
      481 gagtgtagaa ggtctggtgt ttgttgatga ttatgtcggg attgatgtcc ttgagccttt
      541 cctctgcaac atcaaccttg tatttgccta ctgtttttct tgttgcaaaa atctggcgat
      601 tgatgtttgt aaggcacacc ttgtcatcgt caataaggtc aagctcgccg actccgctcc
      661 ttgcaagagc ctccgccgtg tatccgccta caccgccgat tccaaaaatt gcaactcttg
      721 tttttgaaag cctgtccata gcttctttgc caatcaaaag ctgtgttctt gaaaactgat
      781 taagcataaa aacgctcctt aatacatatt caaatcattt agtattccta tcggtacaat
      841 aagtatttac atcggaataa aactcccgct gtttgctgac gggaggtatc tttgtatagt
      901 ttatattgta atcactgttt ttgtcaagca aaacccgaaa tttattgaca tggaacggtg
      961 aatgtagtat aatcaaaaca taccaaacag ataggtttag tggtaaaata actgactaac
     1021 tttaattgac aatagctgtc ggcagtaata aaataacttt aaaagtgtat tttactgtcg
     1081 gattttattt gtaacggagc gaatttttta tgtactgcct gagcgtaagt cacaaaactt
     1141 caaatgtggt tgtgagaaag aagcttgcct ttcccgatga gcaaaaaaag acatttcttg
     1201 acgatcttta ttattctgaa aatatcagcg agtgtttgat tctctgcacc tgcaacagaa
     1261 ccgaggttta tttttgcggt gacgaatcat ctgtgaaaac ggttgaaacc gttctttcgg
     1321 atttttcggg aattgatttt gacgaactaa aaaagtacat ctgtcttttc tacggtgaca
     1381 gggctttgct ccatcttttc agggtggcag gcggaattga atcaatggtt atcggtgaag
     1441 atgaaatact cggtcagcta aagcgagcct acgcctttgc aaaggacaac ggcacggttg
     1501 catatgagct taatatgtgc gttcaggcgg ctgttgcctg tgcaaaaaaa atcaagaccg
     1561 aaaccgccat ttcaaaaaca tcggtttcag ttgcaactct tgcgtcaaac gaggcggcaa
     1621 aattcaaaga cggtgagctg aatgtccttg taatcggtgc aacaggcaag gtcggctcaa
     1681 ctgttgtgaa aaatcttttg tcgcacaaag gtataagcgt taccgtgact tcacgaaaac
     1741 ataaagcaga aactgttttt gaaaatacgg gtgcgaatgt gattgattat gacgaaagat
     1801 acaaagcgtt tgattttgca gactgcgtaa tcagtgccac aagcggaccg cattacacgg
     1861 ttacatatta tgatttaaaa aacaatataa aaaccgaaaa atcaaggctg tttattgacc
     1921 ttgccgtacc gcccgacatt gacgaaaata tccccaaaat aagcggtgta aggctcatta
     1981 acattgactg cataaaacag cttgcaaacg agaacaatgc tctcaaactc gacagcgttg
     2041 aaagtgcaaa agaaataatt tttgaggaaa ttgaagttct caaaaaagac cttgcctttc
     2101 acgaatttct gccgtgtatg cagtctgtaa aaaacgcaat gccgtcggat ttttcggaaa
     2161 aattcattta taagctgaaa tccgatgttt cggcagagga atttgcaaga attcttgaaa
     2221 tctacagaaa gtgcggagag aataactaat ggcatacttc ccgttttata ttgacattga
     2281 aaacaaaaaa atacttgtag tcggcggcgg tacggttgca ctcagaaaga ttgaaaagct
     2341 gacgccgttc tcgccggaca taaccgttgt tgccccgaaa atctgcgatg aaatcaaggc
     2401 tttgaatgta aaaatcattg accgcagatt ttgtgacagc gaccttgacg gtgcgttttg
     2461 cgttgtcagc gcaaccgatg atgaaacgct caacggcaga atttttcagc tttgcaacga
     2521 gaaaaatatt cttgtcaaca cagttgacga taaggaaaaa tgcggattta tttttcctgc
     2581 aattgcaagc aaaaacggca taacggcagg aattacaaca tcgggcaaaa gtccgattta
     2641 cgcaaaatat cttaaagagc tttttgtggg aatccttgaa agtatgaacg aaaatacaac
     2701 cgaggttttg tggaaatatc gcccgataat taaggagaag gttgaaaagg aagatgacag
     2761 aaggaagatt tttgaacagc ttctttcgct ttgcctttcg ggacttgaac ccgatgaaaa
     2821 aactgttgaa aatttgattg aggaatacga acaatgacga taaaaatcgg aacacgaaaa
     2881 agcaggcttg cccttgcaca aaccgaaatg ttaataaaaa cgctgaaaaa ccgttttccg
     2941 aatgttaaaa ttgaaacggt ttacatttcg acaacaggcg acaaaaccct tgacaaaccg
     3001 cttgcagctt taggcggaaa aggtgtgttc atcaaggagc ttgaaatgtc gcttttgagc
     3061 ggagaaacag atgttgccgt tcacagtgca aaggacttgc cgacaaaaat tgcagacgga
     3121 cttgaaattt ccgccgtgtt gccaaggggc agctatggcg atgtgtttgt cacacgcaac
     3181 aattttgaaa caaaggacgg ctttgtcatc ggaacaggca gttcaagacg aaagcttttt
     3241 gcggagaaaa tctatcctaa tgcaaaattc aaggatataa ggggcaatgt tgacacccga
     3301 cttaaaaagc tgttgaacgg tgagtacgat gctcttgtgc ttgcaaaggc aggacttgaa
     3361 agacttgacc tttgcaacgg tgaggattac acggtaactc cgtttgatgc cgatgaattt
     3421 ctcccagcac cgtgtcaggg aatagttgcg attgaaagca gaaaaaacag cgaggtttcc
     3481 aaaatgctcg ctgaaatcag cgacaaaaat acaatgcttt cgtttgaaac cgaaagacag
     3541 gttttgcaca gcctcaatgc cgattgttca actcccgtcg gtgcaatttc aaaggttgaa
     3601 aacgacagga taacgctttc tctgtctgcc gactgtaaaa aaacagtcag cggaacagat
     3661 gaaatttcaa acagaatcaa acttgcaaaa aggctggtga gcgaaattga gtaacggaag
     3721 agtgtatatt gtcggtgcgg gatgcggcga ttttgacctg ataacaatgc gtggtgcgga
     3781 gtatatcaga cattgcgatg tgcttgttta cgacagcctt atagatgtgt cattgctcgg
     3841 ttttgcaccc gaaacggctg aaaaaatctg cgttggaaag cgttcgggca gacacagcga
     3901 aaagcaggaa aatattaacg aaatccttgt tgaaaaggcg agagagggca aaacggttgt
     3961 ccgtcttaaa ggcggcgatc cgttcgtgtt cggcagaggc ggagaggaga ttgaaaccct
     4021 caaaagtgcc gatattccgt ttgacattgt acccggaatt tcttcctcaa ttgccgttcc
     4081 agaacttgcg ggaatccctg ttacacacag aaatctgagc cgaagtgttc atataatcac
     4141 gggacatacg gcacaggaca ccctgcccga aaatataaag aaatgtgccg aaaccgacgg
     4201 aacgcttgtc tttcttatgg gattgagaaa cctccccgac attgtggaaa atctcattcg
     4261 taacggtaaa tcggaggata ctcccgttgc agttgtgtca aacggtgctt gtgcaaaaaa
     4321 tcagaccgtg aggggaactc tctcgacaat ctgcgaaaat gttaaatccg cagaggttgt
     4381 accgcctgcc gttatagtgg ttggcgaaac cgcaaaattt gactttgccc ccacaattac
     4441 aagacctttg aaaaatgttt cggttaccgt tacgggtacg agaaagctct ccgacaagct
     4501 cggcaaaatg ctcacgcttt cgggtgcgga ggtcaaaagg cacgaccttt taaaggtgat
     4561 tgaatatcat gacaacgaag cttttgacaa tgcaataaac ggcattgacg gctacgatta
     4621 tgtggtgctt acaagtatga acggtgcaga aatttttatg agccgactgc gtaagctgaa
     4681 aaaggatatc cgcagttttg caaatgtcaa gtttgcggtg attggttcgg gaactgcagc
     4741 tgtacttgaa aagtacggaa tttttgccga ctgtattccg aatgtctaca caacccgtga
     4801 acttggaatt ttgcttgcaa aaacggtgaa aagcggcgaa aaagtattga ttctgcgtgc
     4861 cgaaaacggt tcgcccgaac tcactcggat tttggctgat aacaacattg attttgacga
     4921 tgtcaaaacc tatgacattc agcttgagaa aaagcaggat aattccgttg ttgataccga
     4981 ttttattact tttgcgagtg cgtcgggtgt gaacacattt tttgacggcg gagcaattat
     5041 ttccgacaga acttccgttg tctgcatagg tgaaattacc gccgaggcac ttgaaagtca
     5101 cggcgtaaat tcgttcaaga ttgcaaaaac gaaggacaca gacggaattt tgaaaactat
     5161 aatcaaggaa gtaactttga aagaaggata aatatatgag aagattcaga agattaagag
     5221 caggcgagca gattcgcagt cttgttcgtg aaacaagaat tgacaagggc gatttgattt
     5281 acccgatttt cattgcagag ggtgaaaata ttaaaaagcc tgttgattca atgccgaatg
     5341 tctaccaata ttcgcttgac agagcagacg agattcttaa agaaattgaa aacagcggca
     5401 ttgcaggact tcttattttc ggaattccaa agcataaaga cgagcttgca acatcagcct
     5461 atgacgataa cggtgtaact cagaacgcaa tcagatacat aaagaaaaat tatccgtcac
     5521 tccttattat tgccgatgtc tgtctttgtg aatacacaag tcacggtcat tgcggtgttg
     5581 tttgcggtca cgaaattctc aatgacgaaa cacttccgct cctctctaaa atggctgtca
     5641 gccttgcaaa agcaggtgcg gatatcatcg ctccttcgga tatgatggac ggcagagtgt
     5701 ctgcaatcag aaacgcactt gacgaaaacg gacttatcaa cactccgatt atgtcataca
     5761 gtgcaaaatt tgcctcaggc tactattcac cgttccgtga cgccgcagaa tctgcccccg
     5821 aatttggcga cagaaaaagc tatcagatgg attacgcaaa cggcaaagag gctctgcgtg
     5881 aaattgcaga tgatattgac gagggtgcgg atatggttat ggtcaagcct gcacttgcat
     5941 atctcgatat cgtaaaagcc gcaagcgaac gctttgacct tccgcttgtt gcctataatg
     6001 taagcggtga atatgcaatg gtcaaggcgg ctgccgaaaa aggctggatt gacgaaaaga
     6061 aaattgtgtg cgaaaatatg attgcaatca agagggcggg tgcggatatc ataatcacat
     6121 accacgcact tgatgttgca aaatggattg acgaatttta caaataggga agtgctttta
     6181 tgaatacaac tcaaagcgaa aagctatacg cacaggcgct tgactttatg cccggcggtg
     6241 tgaacagtcc tgtccgtgcg tgtcgttcgg tcggaagaac tccgcttttt atcgaccgtg
     6301 cagagggttc aaaaatttat gatgttgacg gaaacgagtt cattgactat atctgctcat
     6361 ggggaccgaa tatcctcggt cacaaacagc ccgatgttat caaggccgtg acttctgcct
     6421 gtcagaacgg acttacattc ggtgcgtgcc acagcggaga aattgaactt gccgagctta
     6481 tccgcaaaaa tatgccgtca attgaaatgc tccgacttgt aaattcaggc acagaggcgg
     6541 ttatgagcgc aatccgtgcc gcaagaggct ttacaaaaag ggataaaatc gtaaaattcg
     6601 agggctgtta ccacggccat tccgacggct tgcttgtaaa gggcggttcg ggacttctga
     6661 caaattcaat tccgaacagc gcaggtgtgc cgaaaggcta cacagacaca actctccttg
     6721 caaaatataa cgatgaagaa tccgtgcaac agctttttga taactgcggt gatgaaattg
     6781 cctgtgtaat cgttgagcct tgtgccgcaa atatgggtgt tgttccgccc aaaaaaggat
     6841 ttttaaaatt cttgcgtgaa atcactgaaa agcatgattc acttcttatt ttcgatgaag
     6901 taatcacagg attcagactt tcactcggcg gtgctcaaaa gctttacgga gtaaaacccg
     6961 accttacaac cctcggaaaa attgtcggcg gcggaatgcc gcttgcggtt tacggaggtc
     7021 gaaaagagat tatggagtgt gttgctcctc tcggttctgt ttatcaggca ggcacacttt
     7081 cgggaaatcc ggtagcggta agtgcaggca ttgcaacgct taaaattctt gaaaaaaata
     7141 aagattcgat atatcccgag cttgaacgca agtctgcaaa gattgaaaat gcaatgaaga
     7201 acgcaggact taatgtgaac cgtgtgggtt cgattatgac ggcatttttt accgacaaaa
     7261 aggttgttga ctatgacacg gcaaccacag ccgacacaaa gagatatgcc gagtattaca
     7321 atcatctgct tgaaaacgga atctatgccg caccgtcaca gtttgaggcg atgtttgttt
     7381 catttgcaca cacggatgag gacattgaaa aaacctgtca ggttattgaa agctttaaat
     7441 aaaatccgat aaaaacaaga aaagccaccg acataaaaat cggtggcttt ttgtttgtaa
     7501 tctatcagat gtagtacatc ggaatgttgt gttcaatttc attgtttttg atatactcat
     7561 cgcaaagctg tgcaagtgta atgctttcgg catattcctg aagatatgac gacatcttcg
     7621 accaaagact gttgtcgata agtcttatca taacatcatc atcgccccga gtgttaaaat
     7681 aaacattgct cagaacattt gcgtcaagtg cgtttatgat atccttgaat gtaatctctt
     7741 taaggttttt tgcaataaca taaccgccgt ttgaaccctt ctgacctcgc acaagtcctg
     7801 ccgagctgag tgatgagaga atctgttcaa gatacttttt agatatattt tgccgtttgg
     7861 caatttcggg agttgttacc gcattgccgt ttagtgagtg tgacgcaatg tcaataacag
     7921 cgatgattcc gaattccacc tttgttgaaa tcttcaattt tagcatctcc tttccgtgcg
     7981 ttttgtttgc tgaaagagta tataataatg gtaacagttt ttaatttaaa aatcaatacc
     8041 tactaaacaa atatgcattg taggaaataa tggaattacg gcaaatttta aagaaaaatt
     8101 gtgcaagagg taaaatctta ttaaacctat tgaaatacta tgattaatgg agtataatct
     8161 acctatatca atcgaagccg tcagacaaat tcctacgacg gtcttacaca aaagggttgg
     8221 ttgtatgaaa ttaaggggtt ggtgatatta tggagtttaa caccgcatta cttcacaagg
     8281 gttttaacgg tgaaaaggtt tttggctcaa ccttaactcc catttatcag gtcagtgcat
     8341 tttcgcacag ctctgccgaa cagcttgaaa aggtgttcaa caacagagct cccggctacg
     8401 cctactctcg aataggcaat cccacggtaa catcttttga gaacagaatt gcttccattg
     8461 aaaacggtgt cggtgcggtt gcgtgttcgt caggtatggc agccgttacg atggcactcc
     8521 tgaacattct tgaatcaggt gacgaagtaa tcgcaagcgc aggtcttttt ggcggaacaa
     8581 ttgatttgtt tggagatctt gagcctttcg gaattgttac acgatatgtc aacaaggtga
     8641 cagccgagga gattgaaccg ctcataaacg aaaaaacaaa agcagtcttt gccgagctta
     8701 tcggcaatcc cggacttgaa attgccgatc ttgacgctgt atcagagctt gttcattcac
     8761 acggtgttcc gctcattatc gacagcacaa cggcaactcc gtatcttatt cagccgtttg
     8821 aacacggtgc ggacatcgtt gttcattcct cgtcaaagta cattaacggc ggcggaaatt
     8881 caatcagcgg aataatcgtt gacagcggca attttgaatg gaatacggaa agatacaaag
     8941 gtcttgcaga gtacaaaaaa ttcggtcggc ttgcatatgt tgcaaagctg agaaacggta
     9001 tctggcgaaa cataggctgt tgccttgcac cgtttaatgc ttttatgaac tctgtcggac
     9061 ttgaaacact cggacttcgt atggagcgtc tttgttacaa cgctcttgag cttgcaaagt
     9121 tctttgaaac acaggacgga atcgaagtca actatccggc tcttgaagct tctccatatt
     9181 acagcctttg tcaaaagctg ttgaagggaa aaggcggtgc aatcctcaca atcagggcag
     9241 gaagtaaaga aagagcgttt aagcttatca acaatttgaa gtatgcaaca aatgcaacaa
     9301 atataggcga cacaagaaca cttgtaattc atccgtcaag cacaatttac gctcacggaa
     9361 cagaagaaca aaaaagaaac gcaggcgtgt ttgaggatac aataagaatc agcgtcggca
     9421 ttgaggatat tgaggattta aagtctgact tcaaacaggc tgtagacagt ataaatgtga
     9481 atgaatcgga ggagtaagag aaatggcagt tgattatgct acgcttaaaa agggcggctt
     9541 tatgcgtcaa aaacagaaaa ataatttttc actcaggctc agagttgtag gcggcaacct
     9601 cacagctaaa cagcttgcga aaattgccga ggtttcggaa aactacggtg acggttatgt
     9661 tcacctcaca tcaagacaaa gtgttgaaat tccttttgta aagcttgatg atgttgaggc
     9721 agttaaggac gcacttgccg agggcgatgt tgagccgggc gtttgcggcc cgagagtaag
     9781 aacaattaca gcctgtcagg gtgaggcaat ctgtccaagc ggttgtattg atacatacgc
     9841 tcttgcaaag gagcttgacg acagatattt tgcaaaggaa cttccgcaca agttcaagtt
     9901 cggcataaca ggctgtcaga acaactgcct taaagctgag gaaaacgatg tcggtatcaa
     9961 gggcggaatc aaggtttcat ggaaagagga cgcttgtatc ggctgcggag tctgtgtaaa
    10021 ggcctgccgt cagggtgcaa tcactcttga ggacgggaaa atttccgttg acaattcaaa
    10081 gtgtaactac tgcggcagat gcttcaaggc ttgcccgaca gatgcgtggg acacaattca
    10141 cggctacatt gtttcattcg gcggactttt cggcaactca atcaacaagg gtgaaacaat
    10201 tattccgttt atcgaaaata aagaaaaact tatggaaatc tgtgatgccg ctcttaactt
    10261 ctttgcagaa aacgcaaagc cgagcgaacg ctttaagttt acaatcgaca gagtaggcag
    10321 agaaaaattt gaacagagaa ttttggaggt ttacaatggc tgattttgta attgacgata
    10381 cagtagatat taccgatgtg gtatgcccga caacttttgt aaaggcaaag gttgcccttg
    10441 aagaacttga cgaaggtcag atcctttcaa tcaaaatgaa tgacggcgag cctgttcaga
    10501 atgttccgag aagtatcaag gaagagggac acaaaattct caagctcatt gacaacgagg
    10561 acggcacata tgacctgatt gttgaaaagg ttggtgatta aatgccgcag agagtaagca
    10621 aagacaattt tcagagcgaa gttctttcag ccgatacact cgtgcttgcg gatttttatt
    10681 ccgacagctg tatcccttgc aaaagacttt cacccgtact ctttgagctt gaaaaagagt
    10741 atgagggcaa gctgaaaatt gcaaagataa atgttaattt tgattccgag cttgcatcgg
    10801 aatatgaagt acaggcggca cccacactcg ttttcttcaa aaacggtgaa gagaaagaaa
    10861 gactcagagg tgcggtcaaa aaatcagaaa taatcgaagt gacagaaaaa cttattaagg
    10921 agtaatcgtt atgacaatca cagttgcagg aaacaaaaag gaagttgcag acaatcttac
    10981 ggtagcacag ctcattattg acgagaatgt tgaaacaccc gagtatgtaa ccgttacaat
    11041 caatgacgaa tttgttaaga gcggtacatt tgaagaaaca accttaaagg acggcgatgt
    11101 tgtagagttc ctttacttta tgggaggcgg tcagtaatgg cattttcaaa cgaacagctt
    11161 gaaagatatt caagacacat tattcttaaa gaggtcggcg caaagggaca gaaaaagctc
    11221 cttaatgcaa aggttctcat tatcggtgca ggcggactcg gcgctcctgc cgcaatgtat
    11281 cttgccgccg caggtgtagg tacaatcgga attgcagatg cagacgaggt ggatctctca
    11341 aatcttcaaa gacagattat ccactcaaca caggatatcg gcaagcctaa ggttgagtcg
    11401 gcaaaggaaa caatggaggc tatcaacccc gatgttaccg taaagacata ccacacattt
    11461 gttacaagcg aaaacatact tgaccttgta aaggagtatg acttcatcat tgacggtacg
    11521 gataactttc cggcaaagtt ccttatcaac gatgcctgtg taatggctaa aaagcctttc
    11581 tcacacgccg gaatcatcag atttaaaggt cagcttatga cctatgtgcc gggtgaaggc
    11641 ccttgttacc gttgtgtatt caagaaccct ccgccaaagg acgcagttcc aacctgtaag
    11701 caggcaggtg ttatcggtgc aatgggcggt gtaatcggca gcctacaggc aatggaagca
    11761 atcaagtaca tcatcggtca gggcgagttg ctcacgggct atcttcttac atatgacgca
    11821 ctcaaaatgg aatttcgcaa ggtaaaactt ccgagtgaca cacacaattg tgcagtctgc
    11881 ggtgaccatc cgacaatcac agagcttatc gactacgaac aggcagaatg tgacggtgtt
    11941 catttatgat aaaacttaca aaaagtgact ttgaaaaaat acttgctcac gctgtaaagg
    12001 agcttcccga tgaggcttgc ggtcttattg caggaacggt tgagggcggt gacaaggaga
    12061 ttaaaaaggt ttatctcctt acaaatatcg accactcaaa cgaacatttt tcgcttgatc
    12121 caaaggaaca gcttgaggca atcaaagata tgagaaagaa cggatttgtt cctctcggca
    12181 actggcattc acatcccgaa tcgccgtcaa gaccgtctga agaggacaaa agacttgcct
    12241 ttgacagcaa ggcaagctat atgattcttt cgcttatgga cagagaaaat cccgttctca
    12301 attcattcaa aattacggga gatacagcag aaaaggaaga acttgttata gagtaaagga
    12361 gggagattat gtacgacatc gtgattatcg gcagcggccc cgcaggactt tcagccgcaa
    12421 tttatgcaca gcgtgaaaac cttaaagcgg ttgtggttga aaaagaattt atgggtacgg
    12481 gacagattgc cgagagcgag agagtagata attaccccgg actttacggt gaaacgggct
    12541 ttgacctcgg agaaaagttc agaactcacg ccgaaaaact cggtgctgaa tttgttgagg
    12601 gagaggttac cgaggttgaa agagttaacg gtaacttctc cgtaaacatt gacggcggtg
    12661 aaagtcttga aacaaaaacc gtaatctatg cggcaggaac acagcgcaga aagcttggtg
    12721 taaaaggcga aaaggaattt tcgggtaacg gagtttctta ttgtgcggtg tgtgatgcgg
    12781 ctttctataa ggataaaatt accgtggtaa caggcggcgg tgacactgct ttgggtgacg
    12841 cacttctgct ttcaagattc tgcaaaaagg tttatctcgt tcacagaagg gattccttca
    12901 gagcaggcag agccttgcag aacagggtgt ttgccacgga aaatattgaa gctcttacaa
    12961 atgcaacggt atctgaaatt aacggagata caaaggtgac ttcggtcaca cttttacaaa
    13021 acggagaaga aaaacagctt gattgcaacg gtgttttcgt tgcagtcgga agtgttcccg
    13081 atacaaaacc tcttgcaaat cttgtcaaaa ccgacaagga cggttacatt attgccgatg
    13141 aaagcggaaa aacctccgca gacggtattt ttgcaggcgg tgacatcaga acaaaagcac
    13201 ttcgtcaggt cgttacagcc gtgtcggacg gtgcaaactg tgtgatttcg gcagaaaaat
    13261 atcttgacga aaaacaataa cggcaaggga gtgaaatcct atgaaaacag aaaaaataag
    13321 caccgatgtc ctcattatag gcggcggaac ggcaggctgt tatgcggctc tgaccatttc
    13381 cgaaaattcg gacaaaaagg ttcttatctg tgaaaaagca cacatcaaaa gaagcggttg
    13441 tcttgccgca ggtgtaaatg ctcttaatgc atacattgtt gagggcagaa aaccgcagga
    13501 ctatgttgac tacgctaaaa aggacgccga cgggattgtc agagaggatt tgctccttac
    13561 catgtccgaa aagctcaacg aagtaaccga caggcttgaa aaactcggtc ttgtaatttt
    13621 aaaggacgag aacggaaagt atgttacacg aggcaaccga aaccttaaaa tcaacggtga
    13681 aaacataaag ccgattcttg cggacgcagt cgaaaaggca aagaatgtaa cggttctcaa
    13741 ccgtgttaat atttttgatt actcggtaaa ggacaacaaa atcaacggtg cattcggatt
    13801 cggcattgaa agcggaattt tctacacaat cgaagcaaag gctgtcataa ttgccacagg
    13861 cggtgcggca ggactttaca agccgaacaa ccccggattt tcccgtcaca agatgtggta
    13921 tccgcccttt aatacaggtg caggctacgc aatgggaatc agagccggtg cagagatgac
    13981 aacctttgaa atgcgtttta tcgcactcag atgcaaggac acaatcgctc ccacgggaac
    14041 acttgcacag ggtgtaggtg cagagcagat taattcactc ggcgaggttt atgaaacaaa
    14101 gtacggactt acaacttccg aaagagttta cggtacggta aatgaaaatc aggagggcag
    14161 aggtccctgc tacctcagaa cagagggaat cacagccgag caggacgaat cacttttaaa
    14221 agcctatctc aatatggcac cttcgcagac aatcaagtgg attgaaagcg gaagaaatcc
    14281 ttcacggcag aatgttgaaa ttgagggaac agagccgtat atcgtaggcg gtcacacggc
    14341 aagcggttac tgggttgaca cagaccgtgc aaccacaatt gaaggacttt ttgcaggcgg
    14401 tgatgttgca ggcggttgtc cgcagaagta tgttacgggc gctcttgcag agggtgaaat
    14461 tgcagggctt tcggcagtta agtatattga cagcaaagaa agctttgaga aaatcagcga
    14521 tgaagataca aattatcacc tcagggaaac cgagaaatat ctcacagacc gccattcact
    14581 ctacaccacg gaacagcttg aagaggcaat gcagacggtt atggacagct acgcaggcgg
    14641 tataaaaaca aactaccgtt tcaacgaaaa acagcttgac attgccgact gcaaaatcag
    14701 acagcttgaa acgctcacag atgacctcta tgcagaagat tttcaggagc ttatgtatat
    14761 ctgcgagctt aaagaaaggc ttacggtctg caaaagcgta attgctcacc tcagagcgag
    14821 aaaggaaaca cgctggcaca gttttgccga aaaccttgac tatcccgaaa aggacgacag
    14881 gaactttaac aagtatgtta attcccgtct tgaaaacggc gagataaaaa ttattattcg
    14941 tgaccttgta acgggaggtg aaaagtatga gcatagtaat tgacaagtcc aagtgcatag
    15001 gctgcaaaaa gtgtcgcaat gtctgccccg gaagtcttat caagactgac gaaaacggca
    15061 aagcgtacat caaatatccc aaggattgct ggggctgcac ctcctgcata aaagaatgtc
    15121 ccgcatatgc aatcaacttt ttcctcggtt ctgatatcgg cggtatgggc agtaaggttc
    15181 acaccgaaaa gaacggagat atcctctcgt ggttaattga aaaaccgcat ggcgaggtca
    15241 ttaagattga tataaatcaa aaagaatcaa acaaatacta aggaaacact ttgtaagtat
    15301 ttccgtgaaa aggagataag gtatgagtca gctttcacat ttagatgagc ttgaagcaga
    15361 agctatatac ataatcagag aagttgcggc agagtgcgaa aagcctgtta tgctctactc
    15421 aataggcaag gacagctcgg ttatgctcca ccttgcaatg aaggcgtttt atcccgaaaa
    15481 accgccgttc ccgttcctcc acattgacac aacatggaag ttcaaggata tgataaaatt
    15541 ccgtgatgac acagcaaaaa agctcggaat tgaaatgctc gtttacacca atgaagaagg
    15601 tgttaaaaaa ggcatcaacc cgtttgatca cggtgcggct tacacagata ttatgaaaac
    15661 acaggctctc aaacaggcac tcaacaaata cggctttact gcggcattcg gcggcggcag
    15721 acgagatgag gaaaagtcaa gagccaagga aagaattttc tctttcagaa ataaagcaca
    15781 ggcatgggat cctaagaacc agcgccccga gatgtggaag ctttacaaca caaaaatcaa
    15841 taagggcgaa agcattcgtg tgttcccaat ttccaactgg acagaaaagg acatatggca
    15901 gtacattcaa agggaaaaaa ttgacatcgt tccgctttac tttgcggcaa aaagacctgt
    15961 tgtttaccgt gacggcaaca ttattatggt tgatgacgat cgttttccgc tcaaagaggg
    16021 cgaagtaccc gaattgaaat cggttcgttt cagaacactc ggctgttatc ctcttacagg
    16081 cggcatagag tcaactgcaa caactcttga tgaaattatt gacgaaaccc tcagctccgt
    16141 ttcttcggag cgtacctcca gagtaattga caacgaggca cagggcagta tggaacgcag
    16201 aaaaagggag ggttacttct gatgaaaggt ttacttaaat ttattacctg cggcagtgtt
    16261 gatgacggta aatcaacact cataggtcac atactttatg acgcaaagct tatttatgcc
    16321 gatcaggaaa aggctcttga gcttgacagt aaggtcggca gtcgcagcgg tgacattgac
    16381 tattcgcttt tgcttgacgg tcttatggct gagcgtgagc agggcatcac aattgatgtt
    16441 gcttatcgct acttcacaac cgacaaccgc agttttatcg ttgccgacac acccggtcac
    16501 gaggaatata caagaaacat ggctgtaggc gcatcttttg cagacctcgc agttatcctc
    16561 attgacgcat cacagggcgt tcttgttcag acaagacggc acgcaagaat ctgtaagctt
    16621 atgggtataa gacattttgt ctttgccgtt aataagatgg atttggtaaa gtacagcaag
    16681 tcacgctttg acgaaatcgt aaagcagatt gaagaactta aaaacgagct tttacttgac
    16741 gatatctaca tcattccgct ttccgcaaca gagggcgaca atgttaccgt aaagtccgaa
    16801 aacattccgt ggtacaacgg tgttcctctc cttcagtatc tcgaaactgt tgatgttgat
    16861 tcctcggagg aggaagaagg attctatctt cctgttcagc gagtttgccg tcccgaccac
    16921 acattcagag gctttcaggg acagattgag tcgggcagta tcagcatagg cgatgaaatc
    16981 gtaacactcc cgagcaatga aaaggctcat gtaaagcaga ttcttatgac agataaggat
    17041 gttaaaactg ctttcaaggg acagcccgtt acaatcacac ttgacaagga agttgatgta
    17101 tcccgtggct gtgtaattac aaaggacaca aatcttgcgt cctatcagga gcttacagcg
    17161 tcaattctct ggatggatga cgaacagctt acagccggca aggattatct tgtaaagctc
    17221 ggcacaaaga ctatttcggg tatcgtttcc gaaatcaagt acgctgttga tgtgaacaca
    17281 ggcgagcata ttcctgccga ttcactcaca aagaacggaa tcgcagtctg cacaattctg
    17341 cttgcagagc cgattgcggt tgaccttttc tcaaagcata aaacactcgg tgagcttatt
    17401 ttgattgacc gtgtgtcaaa tatgacatca gcctgcggtg ttgttgattc ggttgaagaa
    17461 aaagccgatg atgcaaaaaa ggcgtcattc gtactcggct cacttgaggc aagaggtgat
    17521 atctttgaag agtttttcta cgacacttca agccttaatg ttctcaaata tcagcctgtt
    17581 aaggaaacct atacaaaggg ggacacaatc cctgttgagg gcgaaagcta taagtatccc
    17641 gacagctttg atattatagt tcttcgtgac agcgttgccg taaaggttcg tgaccgcaag
    17701 attaccgata tcgttccgac atcggagtac agctacggcg gtgttcctgt tgtaaacggc
    17761 agaggcttcg aggttcttgc cgacagcaac gaaaaaattc agcagtttct ttccgagtat
    17821 tcaaatctta aaagcatcaa cgatgcggat ttctttgcaa agtgggttaa gttcgatacc
    17881 tacagaaaaa ttgcaattca aaacagataa caggtttatt cattttaact cattcgcaga
    17941 gggcattgtc atttggcaat gtcctttgtg attttatgac tttcttgccg aaaaactgac
    18001 cttgctgcca aattgttgca tttataattt ttattctgta tagtgtactc aacaaataaa
    18061 ttttaaggag tgcttacaaa tgagcaaaac agcggcggta ctaatcacat tttttctcgg
    18121 tgaactcggt gttcacagat ttatgacagg caaaatcgga acaggtatca tctggctttt
    18181 aacttgcggt gttttcggta tcggttggct tgttgatttt attatggttc ttacaggcaa
    18241 attcacggac aaaaacggaa atgtctgggg tgctaactaa tctgaaagga agttactatg
    18301 gcattcggag ttaaaaccaa aaacattaag acacaaaatt cttatacgat tgagagcttt
    18361 tttgatgcaa tcaaagacaa agagtttacg gcaggaactc cgtcacttac aaagcatgga
    18421 atggctaccg taataacatt tcccgctctc gacagtcaga atcaggtgtg gattatgaat
    18481 aacggtttcg gcaaggaaac gcagaaattc tcaattcaaa agtccactca ggcgggtatg
    18541 gacaatgtag ccaaaaatgc ggcattggat tcaatcacag gcgggctttt cagcgttggc
    18601 ggaatgatca gcgaaaacac aaaaaagtgt gaaaaacttg ttgatgcaac cgcaaaagaa
    18661 ctttcagaaa tgaatctgta accaaagtcg gcacgatttt cgtgtcgatt ttttcttgcc
    18721 aaaaactgcc tttaacggta caattaggtg gggtgagatt atgtttttta acgattatta
    18781 caattctccg attggcaata taactatggc atctgacggt gagagccttt gcggtttgtg
    18841 gtttgatgga caaaagtact ttgcgtcaac cgtaaaaggt gaaactgagg ataaaaccct
    18901 gcctgttttt gaacagacaa aaaagtggct tgacatatat ttttcgggca acgaacccga
    18961 ttttatgccc aaactttcgc ttaacggctc agaatttcgc aaggctgtgt gggatattct
    19021 cctgacaatt ccctacggct cagttatgac ctacggcgaa attgcaaaaa ttttggcaaa
    19081 acagcgtggc gttgccaaga tgtccgcaca ggcggtcggt ggtgctgtgg gtcacaatcc
    19141 gatttcgata atcgtgccgt gtcaccgtgt tgtcgggaca aacggaaacc ttacgggtta
    19201 tgcgggcggc atagacaaaa aaatcagcct tctcaaaatt gaaaagactg atgttaaaaa
    19261 gcttattcaa aaattcccga aaagaattaa ctaaaattat taattaccca acccgtggtt
    19321 attgcaagtc tgtcatccga caggcttgtt tcaattttac cgccgttttt ttcggcaaga
    19381 tttttcaaag ttttatacgc aaagctgtgg tacagaacat tgttctgtgc cttttttatt
    19441 tcattttcaa tcggaaagct tatgcagcaa aagagaaatc cagcccttgt ttctgctttt
    19501 aaggcgataa tttgttttcc gtttccgcaa aattccttta cattttcaaa cgcaatgtca
    19561 atcatattgt taaacaaaac gcagatttca aggttgtcgg caggcacatt tgcaagcgaa
    19621 ccctcaaatg tgattttaat attttcatct gcatttttct gcttatcgct taaaatcgca
    19681 tcagtcagat cattgcccgt gttgtacaaa atctgaaatg agcttataat ctcgctgtca
    19741 tatttttgca ggagaggaag aacctcatca tatttccgac tgttgataag cgattttaat
    19801 ccgattgaca gatcgttata atcatgcctg atttttctca gttcatatcc cgaagtcgta
    19861 atgctcttgt aataattaat ctgtgcgtta agctgtcttt cgtagatatc gttttcatac
    19921 atatatcggc tcttttttat ctgattgtac aaaagaatcg gtattgaaaa cgagaataca
    19981 atcgacacaa tcaaggtcag aattttgagc gtaatcgttc tgtaatcaga tgacggaatt
    20041 atgtagtatg tgccgaaagt cgccatactt ccgccgagat acagaaaaat caatatcata
    20101 attattgtag gtttggtaat tgactcaaca acaagctttg ttttgatttt cagcggactt
    20161 gacaggatta taaacattcc gaaaacaa
//
LOCUS       DAOMVE010000014        19904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_489_length_19904_cov_2.875107, whole genome shotgun sequence.
ACCESSION   DAOMVE010000014 DAOMVE010000000
VERSION     DAOMVE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19904
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_489_length_19904_cov_2.875107"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="PLS64_01670"
     CDS             complement(<1..1991)
                     /locus_tag="PLS64_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HRL42574.1"
                     /translation="MKTKSKILSAILSVSILSASLSAFVSGSLGVQAAESSPTVSANK
                     YRLKDNIQDGVILHCFDWTYNDIKAELPKIAKAGFTSIQTSPAQPNGTGTWYWLYQPI
                     SFSIGTNGVGTKAELQSLCDEAEKYGIKIIVDVVANHLRGDHNNIDNDLKPSEYWHTF
                     GGGIDWKNRWQVTHGSIGMPDIATENPYVQQKVCNYVQELKSVGVDGLRWDAAKHIGV
                     PSEGDDFWKSVTQYGLYNYGEILGGPDDRSTGNEDIMKEYTDYISVTDSNYGKELRDS
                     FNSGKAPTSSGNWSEKGISNDKLLYWGESHDTWSNNKDWGFSNEMSQNVIDRAYAVAA
                     SRNKVTALYFSRPSSTNKESIKMGEKGSTHYTSSEVAQINKFHNAMDGKADYYTVSDG
                     CSVITRKDGGAVIVKGSGSGEVSVENGGGYAKPGTYTDAVSGNTFTITSSTISGTIGS
                     SGIAVVYDAEPEGPSASVTPGSTNYNTDELTLTLNCKNAKNAQYSIDDGAFVNYTNGQ
                     KITIGTNLAYDTVTTVTVKASDGKTTSDPETYTYTKVDPNAVKVVAYDNSSTKWSKVN
                     AYFWSDDNKEMTSWPGKKMTDKGNNIFDIEVPDGAEYVIFNNGNSQTDDLRIADGNKI
                     YSNGSWQNYSTVKPTQPTTAPSTTVRPTTIATQPSTTQP"
     gene            complement(2221..2566)
                     /gene="rnpB"
                     /locus_tag="PLS64_01675"
     ncRNA           complement(2221..2566)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLS64_01675"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2683..3855
                     /locus_tag="PLS64_01680"
     CDS             2683..3855
                     /locus_tag="PLS64_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRL42575.1"
                     /translation="MDIRKESLKKHYEWNGKIEVVSRVPINSAEDLSLAYTPGVAEPC
                     LEIQKDYDKSFELTRRNNLVAVVTDGTAVLGLGDIGPEAGMPVMEGKCALFKEFGDVD
                     AFPICVRSKDVDEIVNAIYLISGSFGGINLEDIAAPRCFEIEKKLKEKCDIPIFHDDQ
                     HGTAVIVAAGLINSLKLVHKKLDEIKVVMNGAGAAGIAIAKHLLNMGVKDITLCDSKG
                     IICKGDPRLNAVKQEMAEITNLSHLEGSLADAMKGADVFLGISAAGCVSEEMVKSMAK
                     DPILFAMANPTPEIMPDIAKKAGAAVVGTGRSDFPNQINNVLAFPGIFRGALDCRASD
                     INEEMKVAASFAIASLVSDEELNADYILPEAFDKRIGKTVAEAVKKAAIESGVARI"
     gene            3904..>4644
                     /locus_tag="PLS64_01685"
     CDS             3904..>4644
                     /locus_tag="PLS64_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRL42576.1"
                     /translation="MKKTVTLLVLISMLFSTVFLFTGCGDSSVKEIKTADDIKGASVG
                     VQTGTTGDTFVSDYEADGTKVMRYSKGADAIVALTQNKIDCVVIDSEPAKEFVKANAG
                     LKILDEPFAEEEYAICISKENHELTEKIDKALAELKDEGVLDKIKSYYIEGNTDTVPY
                     ESPKDIKYDGELHMATNAAFPPYEYVEGGKIVGLDPMMATAICDKLGKKLVIDDMEFD
                     SVITAVQTGKDDFGMAGMTVTDERLQSIN"
     assembly_gap    4645..4654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4655..5407
                     /locus_tag="PLS64_01690"
     CDS             <4655..5407
                     /locus_tag="PLS64_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL42577.1"
                     /translation="IIVNDSASGSLFGNLGESFKNTFITDNRWQQLLSGLLVTLEITL
                     FAGIIGVIIGFVIALVRATHDIQLDKRKCKSFGDCVLKFFNAICNIYITVIRGTPVVV
                     QLMIMYYIILASVRNGIFAAIIAFGMNSAAYVAEIVRSGIMAVDIGQTEASRSLGFNN
                     RQTMTMVVLPQAIKNVLPALGNEIITLLKETSVAGYVALADITYIGNMIRSRTYEAFF
                     PLITVALIYLVIVLVLSYILRRFERRLKKSER"
     gene            5397..6137
                     /locus_tag="PLS64_01695"
     CDS             5397..6137
                     /locus_tag="PLS64_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRL42578.1"
                     /translation="MKDNALISVRGLEKSFGKDIQVLKGIDIDIFKGDVVVVLGPSGS
                     GKSTFLRCLNRLEEPTGGHIFFEGTDITDPKVNINVHRQKMGMVFQQFNLFPHKTVID
                     NLTMAPVKLLGKSKDEAKKDAMDLLERVGLAEKANAYPNQLSGGQKQRIAIVRALAMN
                     PDVLLFDEPTSALDPEMVGEVLDVMKELAKAGMTMVVVTHEIGFAKEVATDVVFIDDG
                     VIKEKNNPQEFFNNPQDDRLKEFLSKVL"
     gene            complement(6190..7536)
                     /locus_tag="PLS64_01700"
     CDS             complement(6190..7536)
                     /locus_tag="PLS64_01700"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRL42579.1"
                     /translation="MQINKNAEKLIRQLNNQGYEAFIVGGCVRDYLLGLTPHDTDICT
                     NALPEQTKKCFEAYHTFDTGIKHGTISVVCGDEVYEITTYRIDGDYSDNRHPDSVSFT
                     RNINEDLQRRDFTVNAMAYNAEYGLVDPYGGKNDLKDKIIRCVGNPDTRFNEDALRIL
                     RALRFASVYGFSIEENTSKSIFKNADLLKNIATERVISEFLKLICGKDAVKILDAYRE
                     TIAVIIPEITVMFNFNQNNIHHSYDLWQHTLTALGTIEPNPILRMTMLLHDIGKPSVK
                     TTDNSGQSHFQGHQLESKKIADKILHRLRLPSDFINKTLLLIEYHDVRFNGSKKLMKK
                     VMNVLGTDLTKQLLEVEYADISAQSEYQREEKLGYLKTAKTHLNEIIKDNECFKLSQL
                     DINGKDVLNLGVKEGRDVGNILDYLLMLVVDANVPNKKSILISKAKEYIYGNQINK"
     gene            complement(7514..9901)
                     /locus_tag="PLS64_01705"
     CDS             complement(7514..9901)
                     /locus_tag="PLS64_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRL42580.1"
                     /translation="MENTNSNLVGLTDSEVQKRIDEGKVNISTNIKTKSIKRIFCDNI
                     FTLFNLINVILLAALIFVGSHKNMLFIGVVIANIIIGIVQEIRSKISVDKLTILSEKK
                     INVLRNGKIAEISKDEIVLDDILVLSRGSQIPADCIVCDGNCRVNESLLTGESNLIEK
                     NVGDELLSGSFIAAGKCYAQAVKVGADCYAAKINNEAKYIKKVNSQIMESFNFIIKIC
                     TFVLFPIGIAFFIRQFTLPDATLQSAVISTVASLVGMIPKGMILLTSSVLAVSIIRLS
                     SKKVLVQEMYCIETLARVDVLCLDKTGTLTTDEMTVTGVIPFGADENKIKKALSSIVK
                     ASDELNATLYALRDYSDSVSPYECSKFCDFSSETKWSGGTFENGKTYIVGATECILKS
                     REKYSEVYSEIEKINQTVRILVLAESDSEPDKDSLPDDLKPLALILIKDKLRDNVNET
                     VKYFKEQGVTLKVISGDSVKTVKNIAIDTGIEGAENAIDMSTVTTDKELEDAAERCNV
                     FGRVTPAQKKKLVVALKKHGHSVAMTGDGVNDVLALKEADCSVAMASGSDAARNVSQL
                     VLVNNDFGAMPSVVAEGRRTINNLERSSALYLVKTIYSVILSIFFIFFRTGYPFEPIQ
                     LTLVGALTVGLPSFVLALQPNKDIVKGNFTVNIIARSLPTAFCISADTILLAILKYMT
                     DMPQAQFSTLCVYLTAFSCMLLVIRLSIPFNPLRAVMVIGCSAGIIIGSIFFGGLFSL
                     APLTFENIILLVIFAVGTFVVFNILYNIAQHYIEKFKKESHLKLTAKRNANKQKR"
     gene            10077..11099
                     /gene="spoIVB"
                     /locus_tag="PLS64_01710"
     CDS             10077..11099
                     /gene="spoIVB"
                     /locus_tag="PLS64_01710"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HRL42581.1"
                     /translation="MRKFKEIISVALIFTLLFTVSTFSPYAADDKNEVYIGGEAFGVK
                     FYANGAMIIKLESYYDGEKYVCPAKDGGLKVNDIIKKVNNTKISTNEELKSEIEKSNG
                     NEITVEIDRNGKTENKTVLPIKNTVGVYLLGAWVRDSCAGIGTITYYNDSNNYFAALG
                     HGICDCDTAALLPLKSGEAVRAEISGIEKSVCGKAGSFCGYFTDVKFGNLSVNSPLGI
                     FGDLTSENYKRNKKYLIANDDEVKTGKAIMVTTPVGDTPQEYDIEIKRICNRDMTSNE
                     NFVIKITDSRLLENCGGIVQGMSGSPIVQNGKIIGAVTHVFLNNPKEGYGVFAQYMAN
                     RYNNQH"
     gene            11248..12018
                     /gene="spo0A"
                     /locus_tag="PLS64_01715"
     CDS             11248..12018
                     /gene="spo0A"
                     /locus_tag="PLS64_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HRL42582.1"
                     /translation="MNNKIKLIVTDDASEFTQENLNILQSKNISVIFCAKDGEELCDK
                     IKRESPDVVLMDSFMTRLDAIGVMRSITRESTKVPVFMVYSSFHSTVLEREIMNSGAS
                     YFVLKPYNISELSTIISDLSDLSKKNNFGKGRVIDAFGIEMKVTDILHEIGVPAHIKG
                     YHYLRDSIIMSVEHPEIINAVTKQLYPSVAKKYETTSSRVERAIRHAIEVAWDRGDID
                     VLNSYFGYTIHNDRGKPTNSEFIAMISDKLRLQIKNAG"
     gene            complement(12055..13320)
                     /gene="purD"
                     /locus_tag="PLS64_01720"
     CDS             complement(12055..13320)
                     /gene="purD"
                     /locus_tag="PLS64_01720"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRL42583.1"
                     /translation="MKILMIGSGGREHALIKKLLESPKCTKLYCAPGNGGISRDAELV
                     NIGVMDKENMVKFAKDNAIDLVFVAPDDPLADGMVDAMQEAGIRAFGPNANAAIIEAS
                     KVFSKNLMKKYGIPTAKYEVFNDAQKAIDYIKNENTAPVVVKADGLALGKGVIIAQSV
                     DEAIEAVKEIMEDKKFGDSGNNIVIEEFLTGPEVSVLAFTDGKCVKPMVSSKDHKRAF
                     DNDEGLNTGGMGTISPNPYYTDEIAKECMDTIFIPTIEAMNKEGRPFKGCLYFGLMIT
                     PNGPKVIEYNARFGDPEAQVVLPRLKTDLVDICEAVIDEKLSDLDIEWDNGAAACVVM
                     ASGGYPVSYKKGIEMFGLNDNGGVDGAIVYHAGTKYENGKFYTNGGRVLGVTATADTL
                     DEALEKAYAAVEKISFEGAHYRHDIGKTK"
     gene            complement(13334..14512)
                     /locus_tag="PLS64_01725"
     CDS             complement(13334..14512)
                     /locus_tag="PLS64_01725"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005334560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HRL42584.1"
                     /translation="MNELALKYGCNPNQKPSRIFMQDGKDLPVEVLNGKPGYINFLDA
                     FNSWQLVKELKAATGLPAAASFKHVSPAGAAVATELSDTLKKIYFVDDLELSPIASAY
                     AMARGADRMSSYGDWVALSDTCDVQTAILLKREVSDGIIAPDYTPEALEVLKSKKKGN
                     YNVVKIDPNYVPAPIEHKDVFGITFEQGRNELKIDEEMLLQNIVTDNKNFTEEAKRDL
                     LVALITLKYTQSNSVCYAKGGQAIGVGAGQQSRIHCTRLAGNKADIWYLRQHPKVMNL
                     PFVDNIRRPDRDNTIDVYISDDYEDVLADGIWQQFFKTKPEPLTKEEKKAWLATFDGV
                     SLGSDAFFPFGDNIERAKRSGVKFVAQPGGSIRDDNVIETCNKYNMTMSFTGIRLFHH
                     "
     gene            complement(14539..15255)
                     /locus_tag="PLS64_01730"
     CDS             complement(14539..15255)
                     /locus_tag="PLS64_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019160976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HRL42585.1"
                     /translation="MKPVSLYNDIASTSYPGRGIVIGKSADGSKAVIAYFIMGRSENS
                     RNRVFAETEDGIKTQAFDPSKLVDPSLIIYSPVRVLGNKTIVTNGDQTDTVYDLMNEG
                     QTFEQSLTTREFEPDGPNYTPRISGIVECENGKMNYAMSILKSADGNPDCCERYTFTY
                     DKPINGLGRFIHTYMSDGNPLPSFEGEPTLVEIGNDDIDTFAEKIWNSLNEDNKVSLF
                     VRYIDIASNKAESRIINKNK"
     gene            complement(15268..15894)
                     /gene="purN"
                     /locus_tag="PLS64_01735"
     CDS             complement(15268..15894)
                     /gene="purN"
                     /locus_tag="PLS64_01735"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRL42586.1"
                     /translation="MKNIAVLVSGGGTNLQALIDAQNRGEIKNGKISLVVSSNPNAYA
                     LERAKNNSIATEVIRRKDYAEFDEYDSAVTELLKSKDVDLVVLAGFMTILGKQFISAF
                     ENRIINIHPSLIPSFCGEGYYGLRVHEEALNRGVKVTGATAHFVNEVCDGGPIIIQKA
                     VEIQNGDTPEILQKRVMEQAEWKILPRAVSLFCEDKIIVKDNKTEILD"
     gene            complement(15894..16931)
                     /gene="purM"
                     /locus_tag="PLS64_01740"
     CDS             complement(15894..16931)
                     /gene="purM"
                     /locus_tag="PLS64_01740"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRL42587.1"
                     /translation="MNNSFSESYKAAGVDVTAGYKSVELMKKHVARTKIDGVISGIGG
                     FGGLFAPDFKDMEEPVLVSGTDGVGTKIKLAFLLDKHDTIGIDAVAMCVNDVICCGAK
                     PLFFLDYIAIGKNYPEKVAEIVSGIAEGCVQSGCALIGGETAEHPGLMPVDEYDVAGF
                     SVGIADKPKMIDGSKLCEGDVLLGLRSSGVHSNGFSLVRKIFDINEETINAEYPELDK
                     TLGETLLTPTKIYVKPLLALMDKVDVKAVSHITGGGFYENIPRMLTDGLSAKIKKDNI
                     PVLPIFKLMQRVGNIPEHDMFNTFNMGVGMIIAVAKEDADKALEVLAANGEDAVVLGE
                     VISGNDGVVFE"
     gene            complement(16945..18399)
                     /gene="purF"
                     /locus_tag="PLS64_01745"
     CDS             complement(16945..18399)
                     /gene="purF"
                     /locus_tag="PLS64_01745"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRL42588.1"
                     /translation="MIKNYVDIDTKLDKVNDECGVFGIYRNDDDIDVVAAANDALFSL
                     QHRGQQSAGITVNKDGEFTTVKELGMVSEIFTPKALEKLPNGKIAVGHVRYTSSESLD
                     RASNQPLVMRYIQGSIGIASNGSITNFNEIRQELETGGAVFQSNSNAEIMAYVIATER
                     CVTDTLEDAVLSSMRKLKGAYSTVICAPSRLIGFRDMHGFRPLCIGKLKNSWIFTSES
                     CVIDSLGGEFVRDVEPGEMVVVDEEGFHSYKLKLLPDKTSFCLFEYVYIARPDSVING
                     VCVHNARFRAGELLYDEFPIEADMVCGVPDSGLIAAQGYAKASGIPFSTGFVKNKYIG
                     RTVGSGKDKKKRLLKTRLTALKANVKGKRVVIIDDSIVRGETSKHIVRLMREAGAAEV
                     HMLISSPPFVCPCYFGVDIHDKESLIANKLTTSEICEYIGADSLGYLSINSLRKIAEG
                     ANIEFCDGCFTGSYDAEIPRTIFVDKYAKKINRK"
     gene            complement(18396..18893)
                     /gene="purE"
                     /locus_tag="PLS64_01750"
     CDS             complement(18396..18893)
                     /gene="purE"
                     /locus_tag="PLS64_01750"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRL42589.1"
                     /translation="MKKVAIIMGSDSDFPVVSKAVSKLKEFGVEYEVHVMSAHRTPDA
                     AIKFSAEAKKNGFGVIIAAAGMAAHLAGVLAAKTTLPVIGIPCKSAQLDGMDALLATV
                     MMPTGIPVATVAVDGAANAAILAVEMLALSDDSLAEKLENMKADMEKGVAEKDKAMQA
                     KVAEL"
     gene            complement(19123..>19904)
                     /locus_tag="PLS64_01755"
     CDS             complement(19123..>19904)
                     /locus_tag="PLS64_01755"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRL42590.1"
                     /translation="QTFESKEDKPFAYGFCFTKLFYLFVIGSFIGTILETIWAFCVDG
                     HFEMRVGMVYGPFIPVYGGGACFLTAALYKLYKLNDTLVFVISAFVGAGFEYFCSWLQ
                     EQMFGTVSWDYSDTPFNLDGRTNLMYALIWGFLGLVWVRYLYPWTAKLIEKIPKRAGA
                     IITTFLIVFMAFNGFMSVTATARWTQRTEGVPASNSFEEYLDEKFDNEKMEFLFPGMK
                     KAAEAGVTTEGIISNERPYLDATDPDNGMKIEQGKDVSEIN"
BASE COUNT         5726 a         4183 c         4020 g         5965 t
ORIGIN      
        1 ataggctgag ttgtagacgg ctgagttgcg attgtagtag gacggactgt cgtggaagga
       61 gctgttgtcg gctgagtggg ttttacagtc gaatagttct gccaagaacc gttgctgtaa
      121 attttattac cgtctgcaat cctgaggtca tctgtctgac tgttgccatt gttgaaaatt
      181 acatattctg caccatcagg cacttcaata tcaaaaatat tattaccctt gtcggtcatt
      241 ttcttgcccg gccacgatgt catttcttta ttatcatcac tccagaaata tgcattgacc
      301 ttagaccact tggttgatga gttatcataa gcaacaacct ttacggcatt cgggtctacc
      361 tttgtgtatg tatatgtttc cggatcggat gttgtcttgc cgtcgcttgc ttttactgtt
      421 actgtggtta cagtatcgta agcaaggtta gtaccgattg taattttctg accgtttgtg
      481 tagtttacaa atgcaccgtc atcaattgaa tactgagcat tttttgcatt cttgcaattg
      541 agcgtaagtg tgagctcatc tgtattgtag tttgttgaac ccggagttac tgacgctgaa
      601 ggtccttcgg gttcggcatc atagactact gcaatacctg atgaacctat tgtacctgaa
      661 atggtgcttg atgtaattgt aaatgtattg cctgaaacag cgtctgtgta tgtacccggt
      721 tttgcgtaac cgccgccgtt ttctaccgat acctcgccgc ttccgctgcc ttttacgata
      781 acagcaccgc cgtctttacg ggtaattaca gaacaaccgt ctgacacggt gtagtaatcg
      841 gctttgccgt ccattgcatt atggaactta ttgatttggg caacctcact gctggtgtaa
      901 tgagttgagc ctttttctcc cattttgata ctttctttgt tggttgaaga aggacgggaa
      961 aaataaagag cagtaacttt attacggctt gccgcaaccg cataggcacg gtcaataaca
     1021 ttctgactca tttcatttga aaagccccag tctttgttgt ttgaccatgt atcatggctt
     1081 tcaccccaat aaagaagctt atcgtttgaa atgccttttt cactccagtt tccgcttgaa
     1141 gtaggagctt tgcccgaatt aaacgaatca cggagttctt taccgtagtt actgtctgtg
     1201 acagaaatat aatcggtata ctctttcata atgtcttcat tgcctgtcga acggtcatcg
     1261 ggaccaccga gaatttcgcc gtagttataa agaccgtact gtgttaccga tttccagaaa
     1321 tcgtcaccct cgctcggaac gccaatgtgt tttgcagcgt cccaacgaag tccgtcaaca
     1381 cctaccgact tgagttcctg aacatagttg cagacttttt gctgaacata tggattttcg
     1441 gttgcaatgt ccggcatacc aatcgagccg tgtgttacct gccaacggtt tttccagtca
     1501 atacctccgc cgaaggtatg ccaatactcg gatggcttta aatcgttgtc tatgttgttg
     1561 tgatcgcctc tcaaatggtt agcgacaaca tcgacaataa tcttaatccc gtacttttct
     1621 gcctcatcac aaagcgactg aagttcagcc tttgtgccga caccatttgt tccgatactg
     1681 aatgaaattg gctgatataa ccaataccaa gtacctgttc cgtttggctg tgccggagaa
     1741 gtctgtatac ttgtaaatcc ggcttttgca attttaggca attcagcctt aatatcgtta
     1801 taagtccagt caaaacaatg taaaattaca ccgtcctgaa tattatcttt gagtctgtac
     1861 ttattagccg atacggttgg cgagctttct gccgcctgaa caccgagtga accggataca
     1921 aaggcagata ggcttgccga caggatgctc acgctcaata ttgcgctgag tatttttgat
     1981 tttgttttca aatttttaca cacctttcaa attaatttca aaactctact atgtgtcgaa
     2041 tcagtcgact gaaacaaatc aaattaaatc aatttcaata gatttcagca taaagcaaag
     2101 tacaataaag agcagcataa atgcaatgca ttttttaaat taattgcaaa tgtgcataac
     2161 atttgtacaa attgctaaaa ttcgacaata atattaaagc acccataaaa ggtgccctaa
     2221 tatgcgactg tgtctgtaag ccgggttttg tcgtgaacag ccatctatct tggcagaccg
     2281 ttaccgaatc tgctcgatgc cacctcctaa ggacacgccg agcaagcgta taatgtccta
     2341 ccacggtgtt gcttcaaata gggtttacac ggcagagcag tctcctgccc tccggtgagc
     2401 tcttacctca cctttccatc cttaccatat aatggcggta attttctgtt gcactatccc
     2461 tggagtcgcc tccggcggcc gttagccgtt attattgctc tgtgaagccc ggactttcct
     2521 cgcacagtac ctttcggcag ctgctcgcag ctgttcaaca cactcgcaca tttattttat
     2581 accttttttg tacaaaagtc aaatgaattt tgtgattttt acttgatata acggcttttt
     2641 aggggtataa tgagagggaa attattataa ggagttaccg atatggatat cagaaaagaa
     2701 tcactcaaaa aacactatga gtggaacggt aagattgaag ttgtttcaag agttccgatt
     2761 aactcggctg aagacctctc tcttgcctat actccgggtg ttgcagaacc gtgtcttgaa
     2821 attcagaagg actatgacaa gtcttttgaa ctcacaagaa gaaacaacct tgttgccgtt
     2881 gtaaccgacg gtacagctgt tctcggtctt ggtgacatag gtcctgaggc aggtatgcct
     2941 gttatggagg gcaaatgtgc gctttttaag gaattcggcg atgttgacgc atttcctatt
     3001 tgcgtaagaa gtaaggatgt tgacgaaatt gtaaacgcaa tttaccttat cagcggttca
     3061 ttcggcggaa tcaatcttga ggatattgcc gctccgagat gctttgaaat tgaaaagaag
     3121 ctcaaagaga agtgtgatat tccgattttc cacgatgacc agcacggtac tgccgttatt
     3181 gttgccgcag gtcttatcaa ctcactcaag cttgttcaca agaagcttga cgaaatcaag
     3241 gttgttatga acggtgcagg tgcggcaggt attgcaattg caaagcacct tcttaatatg
     3301 ggtgtaaagg acattacgct ttgcgacagt aagggtatta tctgcaaggg tgatccgaga
     3361 cttaacgctg ttaaacagga aatggctgaa attactaacc tttctcacct tgagggctcg
     3421 cttgcagatg caatgaaggg tgctgatgta ttcctcggaa tttctgccgc aggttgcgta
     3481 agcgaagaaa tggttaagtc aatggctaag gatcctattc tttttgctat ggctaatccg
     3541 actcctgaga ttatgcctga cattgctaaa aaggcaggtg cagctgttgt aggtacaggc
     3601 agaagtgact ttcctaacca gattaataat gtacttgctt ttccgggtat tttcagaggt
     3661 gctctcgact gccgtgcaag cgatattaac gaagaaatga aagttgccgc atcttttgca
     3721 attgcttcac tcgtgagcga tgaagagctt aatgcagatt atattcttcc cgaggcattt
     3781 gacaagagaa tcggcaaaac tgttgctgag gctgttaaga aggctgcaat tgaatcaggc
     3841 gttgcaagaa tctgataaag atttgcaaat aaatatttta agagaaagaa aaagaggaaa
     3901 acaatgaaaa agacagttac tttattggta ctgatttcaa tgctgttctc cactgttttt
     3961 ctgttcaccg gatgcggtga ttcatcggtt aaggaaatta agactgccga tgatatcaaa
     4021 ggagcatccg taggtgttca gacaggtaca accggcgata cttttgtatc tgactatgag
     4081 gctgacggca ccaaggttat gcgttacagc aagggtgccg acgctatcgt agctcttaca
     4141 cagaataaaa tcgactgtgt tgtaattgac agtgaaccgg caaaggaatt tgtaaaagca
     4201 aatgcaggac taaaaattct tgatgaacct tttgcggaag aggagtatgc aatctgcatt
     4261 tcaaaagaaa atcatgaact caccgaaaaa atcgacaaag ctcttgcaga gcttaaagac
     4321 gagggagttc tggacaaaat caaaagctat tacatagagg gtaataccga taccgtaccc
     4381 tatgaatcac ctaaggatat caagtatgac ggtgaacttc atatggctac gaacgctgct
     4441 tttccaccgt atgaatatgt tgaaggcggt aaaattgtcg gtcttgatcc gatgatggct
     4501 acagcaatct gtgacaagct cggtaaaaaa cttgttattg atgatatgga atttgattct
     4561 gttatcactg ctgttcagac cggcaaagac gatttcggta tggcagggat gacagtaaca
     4621 gatgaaagat tacagtcaat caacnnnnnn nnnnattatc gtaaacgaca gtgcatcggg
     4681 cagtttgttc ggcaatctcg gtgaaagctt taaaaacaca ttcatcacag ataacagatg
     4741 gcagcagctt ttaagcggtt tgcttgtaac ccttgaaatc acattgtttg caggtattat
     4801 cggtgttatt atcggttttg tcattgctct tgtaagagct actcacgata ttcagcttga
     4861 caagagaaaa tgcaagagct tcggtgattg tgttctgaaa ttctttaatg cgatttgcaa
     4921 catctacatt actgttatca gaggcacacc tgttgtagtt cagttgatga ttatgtatta
     4981 cattattctt gcatctgtaa gaaacggtat ctttgccgca atcattgcat ttggtatgaa
     5041 ctctgccgct tatgttgcag aaattgttcg ttcgggtatt atggctgttg acatcggtca
     5101 gactgaggca agccgttcac tcggttttaa caacagacag acaatgacaa tggtcgttct
     5161 tccgcaggca attaaaaatg ttcttcctgc actcggcaat gaaattatta cacttctcaa
     5221 agaaacttcg gttgcaggtt atgttgcact tgcagatatc acatatattg gtaatatgat
     5281 tcgttcaaga acttacgagg cattcttccc gcttattacg gttgcattga tttaccttgt
     5341 aattgtactt gtgctttcgt acatcctcag aagatttgaa aggagattga aaaagagtga
     5401 aagataacgc attaatctcc gtaagaggac ttgaaaaatc attcggtaag gatattcagg
     5461 ttcttaaagg tattgatatt gacattttta aaggcgatgt agttgttgtt ctcggtccgt
     5521 caggctcggg taagtcaact tttctccgtt gccttaacag acttgaagaa ccgacaggcg
     5581 gtcacatttt ctttgagggt accgatatta cggatccaaa ggttaatata aatgttcacc
     5641 gtcaaaaaat gggaatggtg ttccagcagt ttaacctttt cccccacaag actgttattg
     5701 acaaccttac aatggctcct gtcaagcttc ttggcaaaag taaagatgag gctaaaaaag
     5761 acgctatgga tcttttggag cgtgtaggac ttgccgaaaa ggcaaacgca tatccgaacc
     5821 agctttcagg cggtcagaaa cagcgtattg caattgtaag agcgcttgcg atgaatcccg
     5881 atgttctcct ttttgacgag ccgacatctg cacttgaccc cgaaatggtc ggcgaagttc
     5941 ttgatgttat gaaagagctt gccaaggcag gtatgacaat ggttgttgtt actcacgaaa
     6001 tcggctttgc aaaggaagtt gcaaccgatg ttgtatttat tgacgacggt gttatcaaag
     6061 aaaagaacaa tccgcaggag ttcttcaata atccgcagga cgaccgttta aaggaatttt
     6121 tatcaaaggt tctttaattt taaacaaaaa tcagaggatt tcagttttcg ctgaaatcct
     6181 cttttttaat tacttattta tttgattacc gtaaatatat tctttggctt ttgatatgag
     6241 tattgatttt ttgtttggaa catttgcatc tactacaagc attagcagat aatcgagaat
     6301 atttccgaca tctcttccct cttttacgcc aaggtttaaa acatctttac cgttgatatc
     6361 aagctgactc agcttaaaac attcgttatc tttgattatt tcattaagat gtgttttagc
     6421 cgttttaaga tatccgagct tttcttctcg ctgatattca gactgggcag atatatcggc
     6481 gtattcgacc tctaacaact gctttgtaag gtctgtaccg agtacattca taaccttttt
     6541 cataagcttt ttactgccgt taaaacggac atcatgatat tcaataagca gaagagtttt
     6601 gttaataaaa tcagacggta atcttagtct gtgcagaatt ttgtctgcaa tttttttact
     6661 ttcaagctga tgtccctgaa aatgcgattg accgctgttg tcggtggttt taacagaagg
     6721 cttgccgatg tcatgcagaa gcattgtcat acgaagtatg ggattcggtt caattgtgcc
     6781 gagtgcagtc agggtatgtt gccacaaatc atacgaatga tgaatgttgt tctgattgaa
     6841 attgaacata acggtaattt cagggataat aacagcaatc gtttctcggt aagcgtcaag
     6901 aatttttacg gcatccttac cgcatatgag ctttaaaaat tccgaaatca cacgctctgt
     6961 tgcaatgttt tttagcaagt ctgcattttt aaatatagac tttgatgtgt tctcttcaat
     7021 tgaaaatccg taaacagagg caaagcgtaa tgcccgcaag attcgcagag catcctcgtt
     7081 aaagcgtgta tcgggatttc cgacacaacg gataatttta tctttaagat catttttacc
     7141 gccgtatgga tcgacaagac cgtattcagc gttatatgcc attgcattta ccgtaaagtc
     7201 acggcgttgc aaatcttcgt ttatgtttcg tgtaaagctt acgctgtccg gatgacggtt
     7261 atcggaataa tcaccgtcaa tacggtaggt tgtgatttca tagacttcat cgccgcatac
     7321 aacggaaatt gtgccgtgtt tgatacctgt atcaaaagta tgataagcct caaagcattt
     7381 ttttgtctgc tcgggcaaag cgtttgtaca aatatctgta tcatgcggag tcaatccgag
     7441 aagataatca cgaacacaac cgcccactat aaaagcctca tagccttgat tattcagttg
     7501 tctgattaat ttttcagcgt ttttgtttat ttgcatttct ttttgcagtc agtttaagat
     7561 gactttcctt tttgaatttt tctatatagt gctgtgctat attgtagagt atattaaata
     7621 caacaaatgt gcctacagca aagattacaa gcaatatgat gttttcaaat gtaagcggtg
     7681 caagtgagaa caatccgcca aagaagattg aaccgattat tattcctgcg gaacatccga
     7741 tgaccataac ggctctgagc ggattgaacg ggattgataa tctgattaca agcagcatac
     7801 atgaaaatgc agtaagatat acgcagagag ttgaaaactg tgcctgtggc atatctgtca
     7861 tatactttaa aattgcgagt aatattgtat ctgccgaaat acaaaaagct gttgggagtg
     7921 atcttgctat tatatttacg gtgaaattac cttttactat atctttattc ggttgaagtg
     7981 cgagaacaaa tgatggcaat ccaaccgtta atgcaccgac aagcgtaagc tgtatcggtt
     8041 caaagggata tcctgtgcgg aagaatatga agaaaattga gagtataacc gaataaatcg
     8101 tcttaacaag atagagtgca gagcttcgtt caaggttatt gattgttcgt ctgccctctg
     8161 ccacaacgct cggcattgcg ccaaagtcat tattgacaag tacaagctgt gacacatttc
     8221 gtgcggcatc actgcccgac gccatagcta cggagcaatc cgcctctttg agtgcgagaa
     8281 catcatttac tccgtcacct gtcatagcaa ccgaatgtcc gtgcttttta agggcaacca
     8341 caagcttttt cttttgagcc ggagttactc tgccaaacac attacaacgc tctgccgcat
     8401 cttcaagctc tttatctgtt gtcacggttg acatatcgat tgcattttct gcaccctcaa
     8461 ttcctgtatc aattgcaata tttttaacag ttttaacgct gtcgcctgaa attaccttta
     8521 aggtaacacc ctgttcctta aaatatttta ccgtttcatt tacattatca cgaagtttat
     8581 ccttgataag aatgagtgca agcggtttta aatcatcagg caatgagtct ttgtccggtt
     8641 cactatcgct ttcggcaaga acaaggattc tgacggtttg atttatcttt tcaatctcag
     8701 aatacacctc ggaatacttt tctctgcttt ttaaaataca ttcagtcgca ccgactatgt
     8761 aggtcttgcc gttttcaaat gtaccgcccg accattttgt ttcggacgaa aaatcgcaga
     8821 atttactgca ctcatacggt gaaacggaat ccgaatagtc acgcagtgca tacaaagttg
     8881 cattcagttc atcggacgct tttacaattg aagaaagtgc tttcttgatt ttattttcat
     8941 cagccccaaa cggaatgaca cctgttaccg tcatttcgtc tgttgtaagc gttcccgttt
     9001 tatcaagaca aagcacatca actcttgcaa gtgtttcaat gcaatacatt tcttgtacaa
     9061 gcaccttttt ggatgaaagc ctgataattg atactgcaag aacagaactt gtaagaagaa
     9121 tcattccctt tggaatcata ccgacaagcg acgcaacagt gctgattaca gcagactgca
     9181 atgttgcatc tggcaaggtg aattgtcgaa taaaaaatgc aattccgatt gggaaaagta
     9241 caaatgtaca gattttgata atgaaattga atgactccat aatctgagaa tttacttttt
     9301 taatatattt ggcttcgtta tttatctttg cggcatagca atcggctccg acctttacag
     9361 cctgagcata gcattttcct gccgcaataa aactgcccga gagaagttca tctccgacat
     9421 ttttttcaat gagatttgat tcgcctgtaa gcaaactttc gttgactctg cagttgccgt
     9481 cgcatacaat gcagtcggca ggaatctggc ttcctcttga aagcacaaga atatcatcaa
     9541 gaacaatctc atctttactt atttctgcaa ttttaccgtt tctgagaaca ttgatttttt
     9601 tctcagacaa aatagtcagc ttgtcaacgc ttattttgga acgaatttcc tgaactatac
     9661 ctataattat atttgcgata acaacaccga taaaaagcat attcttgtgt gaacccacaa
     9721 aaatcaatgc cgcaagcaaa attacattta taagattgaa aagcgtaaaa atgttatcac
     9781 aaaatattct tttgattgat ttggttttaa tattagttga aatatttacc tttccttcat
     9841 ctatacgctt ttgaacttcg gaatcggtaa gaccgacaag gttagaattt gtgttttcca
     9901 tatatacctc ataataagaa aatgatataa tttcagaaaa taatttacaa tgcacggctg
     9961 tttatcgcct tttcggaaaa tcctctgcca gtaacatttt taatattttc atcaacgcac
    10021 taatctatat tataaatgca aatcataata aaggcaacgg aaagaaggtg aattttttgc
    10081 gaaaatttaa agaaattata tcagttgccc tgattttcac attattattt actgtttcga
    10141 ctttttcgcc ttatgccgct gatgataaaa atgaggttta cattggcggt gaggctttcg
    10201 gagttaaatt ttatgctaac ggagcaatga taatcaagct tgaaagctac tacgacggtg
    10261 aaaaatatgt ttgccccgca aaagacggcg gacttaaagt aaacgatatt ataaagaaag
    10321 taaataatac aaaaatcagc acaaatgaag aattaaagtc tgaaattgaa aaatcaaacg
    10381 gcaatgaaat tactgttgaa attgatagaa acggcaaaac cgaaaacaaa accgttttgc
    10441 cgattaaaaa taccgtggga gtatatctcc tcggtgcgtg ggttcgtgac agctgtgcag
    10501 gcatcggaac tataacctat tataatgaca gcaataatta ttttgccgca ctcggtcatg
    10561 gaatatgcga ctgtgacacg gctgctttgt taccgttaaa atcaggagaa gcggttcggg
    10621 ctgaaatcag cggcattgaa aaaagcgtct gcggtaaagc cggcagtttc tgcggatatt
    10681 ttaccgatgt aaagtttggt aatttgagcg taaactcccc tttaggaatt ttcggagatt
    10741 taacgagtga aaattataaa agaaacaaga aatatctgat tgcgaatgac gatgaagtta
    10801 aaacaggaaa ggctattatg gtaacaacgc ctgtcggaga tacgccacag gaatatgata
    10861 ttgaaatcaa aagaatatgc aaccgtgata tgacatcaaa tgaaaatttt gtcataaaaa
    10921 taaccgacag cagattgctc gaaaattgcg gcggtattgt acagggtatg agcggcagtc
    10981 cgatagttca aaacggtaaa attattggtg ctgttaccca tgtttttctg aataatccca
    11041 aagagggtta cggtgtgttt gcacaatata tggcaaatcg ctacaataat caacactaaa
    11101 tatatgatgg tttgttcatt tttgatttag taacgcaaaa tataaaactt ttttctcaat
    11161 ttttcaaaaa agttttatgg aaggtattgc caactttacc attttgtggt aatattagta
    11221 caacaaaaat tactggggga aatcacaatg aacaacaaaa taaaactaat cgtaaccgat
    11281 gacgcttccg aatttacgca ggaaaatctt aatatcttac aaagtaagaa tatttccgtt
    11341 attttctgtg caaaagacgg agaagaattg tgcgataaaa ttaaaagaga aagccctgat
    11401 gttgtactga tggattcatt tatgacaagg cttgacgcaa tcggtgttat gagaagcatt
    11461 acacgagaaa gcacgaaagt gcctgtgttt atggtgtatt cgtcattcca cagcactgtt
    11521 cttgagcgtg aaattatgaa ttcgggcgca tcatattttg tgttgaagcc gtataacatt
    11581 tccgaattaa gcaccattat cagcgatttg tcggatttga gcaagaaaaa caattttggc
    11641 aaaggcagag ttattgacgc tttcggaatt gaaatgaaag tgaccgatat tctccatgaa
    11701 ataggagttc cggctcacat caaaggctat cactatctgc gtgattcaat cattatgtct
    11761 gttgagcatc ctgaaattat caatgccgtg acaaaacagc tctatccgtc ggttgccaaa
    11821 aagtacgaaa caacttcttc acgggttgaa agagctataa gacacgcaat tgaggttgca
    11881 tgggatcgag gggacattga tgttctgaac tcatatttcg gatatacaat tcacaacgac
    11941 agaggtaagc ccacaaacag cgaatttatt gccatgattt ctgacaaatt aagactgcag
    12001 attaaaaatg caggctaaaa aaagagccgc tcgtaatgag tggctcttaa tatattattt
    12061 agttttgccg atatcgtgtc tgtaatgagc gccttcaaag gaaatttttt ctactgcggc
    12121 atatgccttt tcgagtgcct catcaagtgt atccgctgtc gcagtaacac cgagaactcg
    12181 accgccgttt gtatagaact ttccgttttc gtacttagtg ccggcatgat aaacgattgc
    12241 accatcgaca ccgccgttat cattaagacc gaacatctct ataccctttt tgtatgaaac
    12301 aggatatccg ccgcttgcca taacaacaca agccgcagca ccgttgtccc attcaatgtc
    12361 aaggtcggaa agcttttcgt caataacagc ctcgcagata tctacaaggt cagttttaag
    12421 tcttggaaga acaacctgag cctcaggatc gccgaaacgg gcattgtatt caataacctt
    12481 tggaccgttc ggtgtaatca taagtccaaa gtacagacaa cccttaaaag gtctgccctc
    12541 tttattcatc gcctcaattg tagggataaa gattgtatcc atacattcct tggcaatctc
    12601 gtctgtataa tatgggttcg ggctgattgt gcccataccg cctgtgttaa gaccttcgtc
    12661 attatcaaaa gcacgcttgt ggtcttttga gctgaccata ggttttacgc acttgccgtc
    12721 tgtaaatgca agaacagaaa cctcgggacc tgtaagaaac tcttctataa caatattgtt
    12781 acctgagtca ccaaacttct tgtcctccat aatttctttg actgcttcaa ttgcctcatc
    12841 aacagattga gcaataatta cacccttgcc gagtgcaaga ccgtcagcct ttacaacaac
    12901 gggagcggta ttttcgttct ttatatagtc aattgctttc tgagcgtcat tgaatacttc
    12961 atactttgca gtaggtatgc cgtatttctt cataagattt tttgagaaaa cctttgacgc
    13021 ttcaataata gcggcatttg cattaggacc gaatgctctg attccagcct cctgcatagc
    13081 gtcaaccata ccgtcagcaa gcggatcatc aggagctaca aaaacgaggt caattgcatt
    13141 gtcctttgca aacttaacca tattttcttt atccataact ccgatattaa caagctcggc
    13201 atctcttgaa ataccgccgt tgccgggagc gcaataaagc tttgtgcatt taggactttc
    13261 aagaagcttt ttaattaaag cgtgctctct gccgcccgaa ccaatcatta aaattttcat
    13321 aaccgcacct ctatcagtga tggaaaagac gaatacccgt aaatgacatt gtcatattgt
    13381 acttattgca ggtttcaata acattatcat cacgaattga accgccaggc tgtgcaacaa
    13441 acttaacgcc tgaacgcttt gctctttcaa tgttgtcacc gaatgggaag aatgcgtcag
    13501 aaccgagtga aacaccgtca aatgttgcaa gccaagcctt tttctcttcc tttgtaagag
    13561 gttcgggctt agttttgaag aactgctgcc atataccgtc agcaagcaca tcctcataat
    13621 catcagagat gtaaacatca atagtgttat ctctgtcggg acggcggatg ttgtcaacaa
    13681 acggaaggtt cataaccttc ggatgctgtc tgagatacca gatatcagcc ttattgcctg
    13741 caagtcttgt gcagtgaatt cttgactgct gacctgcgcc tacaccgatt gcctgaccgc
    13801 cctttgcgta gcaaacagaa ttagactgag tatatttaag agtaataaga gcaacaagaa
    13861 ggtcacgctt agcttcctct gtaaaattct tattatctgt tacaatgttc tgaaggagca
    13921 tttcttcgtc aattttgagt tcatttctgc cctgttcaaa tgtaatgccg aaaacatcct
    13981 tatgctcaat aggagcagga acatagttcg ggtcgatttt tacgacatta tagttgcctt
    14041 tcttttttga cttgaggact tcaagagcct cgggagtata gtcaggagca ataataccgt
    14101 cagaaacctc tctcttgagg aggattgcag tctgaacatc gcaagtatcg gaaagagcca
    14161 cccagtcacc gtatgacgac attctgtctg cacctcttgc cattgcatat gcgcttgcaa
    14221 tcggagaaag ctcaaggtca tctacaaagt agattttctt gagtgtgtca gaaagctcag
    14281 ttgcaactgc cgcaccggca ggacttacat gcttaaatga tgcagctgca ggaagacctg
    14341 ttgccgcttt gagttccttt acgagctgcc atgagttaaa tgcatcaaga aagttgatat
    14401 aaccgggttt gccgttgaga acctcaacag gaagatcttt gccgtcctgc atgaaaattc
    14461 tcgaaggctt ctgattcggg ttacagccgt attttaaagc aagttcgttc attggattca
    14521 tcctttccga aaaataaatt acttgttttt attgataatt cttgactctg ccttatttga
    14581 agcaatgtca atatagcgta caaaaagtga aaccttgtta tcctcattga gagaattcca
    14641 gattttctcg gcaaatgtgt caatatcatc gttaccgatt tctacgagtg taggttcacc
    14701 ctcaaagctc ggaagcggat tgccgtcact catatatgtg tgaatgaatc tgccaagacc
    14761 gtttatcggc ttgtcatatg taaatgtgta tctttcgcag caatcgggat tgccgtctgc
    14821 acttttgaga attgacattg catagttcat cttgccgttt tcacactcaa cgatacctga
    14881 aattcgagga gtgtagttag gaccgtcagg ctcaaattct cttgttgtga gtgactgttc
    14941 aaatgtctga ccttcattca taagatcata aacagtatct gtctggtcac cgttagtaac
    15001 gatagttttg ttgccgagaa ctcttacggg agaatagatg ataagtgacg gatcaacaag
    15061 ctttgacgga tcaaaagcct gagttttgat gccgtcctcg gtttctgcaa acactctgtt
    15121 tctgctgttt tcgcttctgc ccataatgaa gtatgcaata acagcctttg agccgtctgc
    15181 acttttgccg attacaatgc ctctgcccgg atatgaagtt gaagcaatgt cattgtaaag
    15241 tgatacaggt ttcatatttt tcctccgtta gtcaagaatt tcggttttat tgtcttttac
    15301 tataatttta tcttcgcaga aaagcgaaac cgcccttgga agaatcttcc attcagcctg
    15361 ttccataact cttttctgaa gaatttcagg tgtgtcaccg ttctgaattt caacggcttt
    15421 ctgaatgata atgggaccgc cgtcgcacac ttcgtttaca aaatgtgcgg tagcacccgt
    15481 taccttgacg cctctgttaa gagcctcctc atgaactctg agtccgtaat agccctcgcc
    15541 gcagaatgac ggaataagcg acggatggat attgattatt ctgttttcaa aagcagaaat
    15601 aaactgcttt ccgagaatag tcataaatcc cgcaagaaca acaaggtcaa catccttgga
    15661 tttcaaaagc tcggtcacag cagaatcata ctcatcaaat tcggcatagt cttttcttct
    15721 gataacctca gttgcaatcg aattgttttt tgctctttca agtgcataag cgtttggatt
    15781 tgaggacaca acaagagaga tttttccgtt tttaatctct cccctgttct gagcgtcaat
    15841 aagtgcctga agatttgtgc cgccgcccga cacaagaact gcaatatttt tcattattca
    15901 aacactactc cgtcattacc gctgattact tcaccgagta caacagcgtc ttcaccgtta
    15961 gccgcaagaa cctcaagagc cttgtctgca tcttccttgg caacggcgat tatcatacct
    16021 acacccatat tgaatgtgtt gaacatatcg tgctcgggga tattacctac tctctgcata
    16081 agcttgaaaa taggaagaac aggaatattg tcctttttga ttttggctga aagaccgtct
    16141 gtaagcatac ggggaatgtt ttcatagaaa ccgccgcccg tgatgtgtga aacagccttt
    16201 acatcaacct tatccataag agcgagaaga ggttttacat aaatttttgt tggagtgagc
    16261 aatgtttcac cgagagtttt gtcaagctca ggatactcgg cattgattgt ttcctcgttg
    16321 atatcaaaga tttttctgac aagcgaaaaa ccgtttgagt gaacacccga agaacggagt
    16381 cctaaaagta catcaccctc acaaagcttt gaaccgtcaa tcattttcgg cttgtctgca
    16441 ataccaacgc tgaaaccagc aacatcatat tcatcaacag gcataagacc cggatgctct
    16501 gcagtttcac cgccgataag agcacaaccg gactgaacac agccttctgc aatgcctgat
    16561 acaatttcag ctaccttttc gggatagttc ttgccgattg cgatataatc gaggaagaaa
    16621 agaggttttg ctccacagca aattacatcg tttacgcaca ttgcaactgc gtcaataccg
    16681 attgtgtcat gcttgtcaag aagaaaagca agcttgattt ttgtacctac accgtcagta
    16741 ccgctgacaa gtacaggctc ttccatatct ttgaaatcag gggcaaaaag tccgccgaaa
    16801 ccgccgatgc ctgaaataac accgtcaatt tttgtgcgtg ccacatgctt tttcataagc
    16861 tctacagact tatagcctgc tgttacatca acacctgccg ctttgtagct ttcggaaaag
    16921 ctgttgttca ttgtaatcac tccgtcactt tctgttaatt tttttagcgt atttatctac
    16981 aaagattgtt ctcggaattt cagcgtcata gctgcccgtg aaacaaccgt cacagaattc
    17041 tatatttgca ccctcagcaa tttttctgag actgtttata ctgagataac caagtgaatc
    17101 tgcaccgata tattcacaga tttcacttgt tgtcaacttg tttgcaataa gactttcttt
    17161 atcgtgaatg tccacaccaa agtagcacgg acatacaaac ggaggtgagc tgatgagcat
    17221 atgcacctcg gcagctcctg cctcacgcat aagacgaaca atatgctttg aggtttcacc
    17281 tctgacgatt gagtcgtcaa taataacaac tctcttgcct ttaacattag ccttcaaagc
    17341 ggtaagtctt gttttgagaa gtcttttctt tttatcttta cccgaaccta ctgttctgcc
    17401 gatatatttg ttcttgacaa aacctgtgct gaacgggata cccgaagcct ttgcataacc
    17461 ctgtgcggca ataagtcccg aatcgggaac accgcacacc atatctgcct caatcgggaa
    17521 ctcgtcataa agaagttcac ctgctctgaa tcttgcatta tgtacgcaga caccgttgat
    17581 tacgctgtcc ggtcttgcga tatatacata ttcaaaaagg cagaaagatg ttttatcggg
    17641 caaaagcttt aatttgtagc tgtgaaagcc ttcttcgtca actactacca tttcgcccgg
    17701 ctcaacatca cggacaaatt caccgccgag actgtcgata acacagcttt cggaagtgaa
    17761 aatccagctg tttttgagtt tgccgataca gagcggacgg aatccgtgca tatcacgaaa
    17821 gccgataaga cggctcggtg cgcaaattac agttgagtaa gcgcccttga gctttctcat
    17881 tgaagaaaga acagcatctt caagcgtgtc tgttacacat ctttcggttg cgatgacata
    17941 agccataatt tcagcattag aattggactg aaaaacagca ccgcctgttt caagctcctg
    18001 ccttatttca ttgaagtttg taattgagcc gttgcttgca ataccgattg aaccctgaat
    18061 atatcgcata acaagcggct ggttagatgc acggtcgagt gattccgatg aagtataacg
    18121 gacatgaccg actgcaatct taccgtttgg aagtttttca agtgctttgg gtgtaaaaat
    18181 ctcggacacc ataccgagtt ccttgacagt tgtaaattca ccgtctttat tcacggtgat
    18241 acctgcactc tgctgtcccc tgtgctgaag gctgaagagt gcgtcatttg cggcggcaac
    18301 aacatcgata tcgtcatcat tacggtaaat accgaacaca ccgcactcgt catttacttt
    18361 gtcaagtttt gtgtcaatat ctacatagtt cttaatcaaa gctctgccac cttagcctgc
    18421 attgccttgt ctttttcggc aacgcctttt tccatatcag ctttcatatt ttcaagcttc
    18481 tctgcaagag aatcgtcgga aagtgcgagc atttcaacgg caagaattgc agcgttagcc
    18541 gcaccgtcaa ctgctactgt tgcaaccgga attcctgtag gcatcataac tgttgcaaga
    18601 agagcatcca taccgtcaag ctgagccgac ttacatggaa ttcctatcac aggaagggtt
    18661 gtttttgccg caagaacacc tgcgagatgt gccgccatac ctgctgcggc aatgattacg
    18721 ccaaaaccgt tctttttagc ctctgccgaa aacttaattg ccgcatcggg agtacggtgt
    18781 gcgctcatta catgaacctc atactctacg ccaaattctt tgagtttact gacagccttt
    18841 gatactacgg ggaaatcact gtcggagccc ataataattg caaccttttt cataattact
    18901 catagccttt ccgcccactt gataaattgc cgcaaaaacc gtgccgtggg cagacaaagt
    18961 caggacaaat atgccaattt atgctttgaa tcgacataaa taaagcatta ctaattaatt
    19021 atataaaaaa atcggttaaa attcaatagt caggcgaaat tatttttaaa ataccgccaa
    19081 aaagcaaaaa gtgactgtgc aaagcatagc cacttcatga tattaattaa tctcggaaac
    19141 atcttttccc tgttcgattt tcatgccgtt atcgggatct gtagcatcaa gatacggtct
    19201 ttcgttggaa attatgccct cggttgtaac tcccgcctct gcagcctttt tcatacccgg
    19261 aaaaagaaat tccatctttt cattatcgaa tttttcatcg agatattctt caaagctgtt
    19321 tgacgcaggc actccctctg ttctctgagt ccatcttgcc gtagctgtta cggacataaa
    19381 gccgttgaat gccatgaata caatcagaaa tgttgtaata attgcacctg ctcttttagg
    19441 gattttttca ataagctttg ctgtccacgg atacagataa cgcacccaga caagaccgag
    19501 gaatccccag ataagggcat acataagatt agttctgccg tcaaggttaa acggagtgtc
    19561 actgtagtcc catgaaaccg tgccgaacat ctgctcctgc aaccacgaac agaaatactc
    19621 aaaacctgct ccgacaaaag cacttatcac aaaaacaagc gtatcattaa gcttgtaaag
    19681 cttatacaaa gcggctgtca gaaaacacgc accgccgccg taaacaggaa taaacggacc
    19741 gtaaaccata ccgacacgca tttcaaaatg tccgtcaacg cagaacgccc atattgtttc
    19801 gagaatcgtg ccgatgaaag aaccgatcac aaacagataa aaaagctttg taaaacagaa
    19861 tccgtaagca aacggcttgt cctctttgct ttcaaaggtt tgtg
//
LOCUS       DAOMVE010000015        19633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_499_length_19633_cov_3.340127, whole genome shotgun sequence.
ACCESSION   DAOMVE010000015 DAOMVE010000000
VERSION     DAOMVE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19633
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_499_length_19633_cov_3.340127"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..2230)
                     /locus_tag="PLS64_01760"
     CDS             complement(146..2230)
                     /locus_tag="PLS64_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL42591.1"
                     /translation="MLRDLKPTDNVGGFDVRPGNFLLNGATTVSGGVNFTIHSVYAVE
                     CTLLLFRPYAKFPYARLRFPDSYKIGNTYSMLVFGLDEIDFEYAYSFDGPYEPEKGII
                     FDKKKYILDPYAKAVIGQSGWGKKQEHEGVYKARVVNSDYDWGNCTQPKLPFEELIIY
                     ELHVRGFTQDGSSGVKNKGTFAGIREKIPYLKELGINAVEMMPIFEFDEMGSYRNYEG
                     RQLYDYWGYNTVCFFAPNTSYESDHKHHHEGRELKQLVRELHENGIEVILDVVFNHTA
                     EGNEMGPYFSFKGIDNNIYYMLTPDGKYYNFSGCGNVLNCNQPIVQQFILDCLRYWVT
                     EYRIDGFRFDLASILGRNEDGTPMDKPPLLKSLAFDPILGGVKLIAEAWDAGGLYQVG
                     SFPSWNRWAEWNGRYRDDLRRFLKGDSHLAWDAAQRITGSRDLYDPTYRGYNASVNFL
                     TCHDGFTLYDMYSYNEKHNLENGWNNTDGANDNNSWNCGAEGDTNDYNINKLRIKMIK
                     NAFATLMCSQGPALFLAGDEFCNTQFGNNNAYCQDNIISWLDWTRKEKHKDVFEFFKY
                     MIAFRKRFHIITDSRGKATCSYPPVSIHSNVAWSAKYYDDTRMIGVMYAGVSLKQQGL
                     DEFVYFGVNAYWDYVNVELPDLPEGYHWKLYVNTGNEPQDVILEDRNVILYDRRINMA
                     GRSVIVAVGERY"
     gene            complement(2447..4141)
                     /locus_tag="PLS64_01765"
     CDS             complement(2447..4141)
                     /locus_tag="PLS64_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HRL42592.1"
                     /translation="MGYINESVIYNIYPLGFCGAPKENDFKQEYRLDKIYGWIDHMKS
                     MSVNALVFNPVFESSKHGYDTIDYKKIDCRLGDNESFKKICKTLHDNGIKIMLDGVFN
                     HVGRDFFAFKDVQQNRENSRYASWFENVNFRNNSPYNDGFSYEGWAGYYDLVKLNLHN
                     DEVVNYLLDCARFWIDEFDIDGLRLDAADCIDIEFFKKLKNVCKEKKSDFWLYGEITH
                     GDYNHWANPDVLDSVTNYECYKGIYSSHNDHNYFEIAHSLNRQFANGGIYRNIYTYNF
                     VDNHDVNRVASMLKDKNHLNNVYTMMYTMPGVPSIYYGSEFGIEGMRTAYSDYELRPC
                     LDLDSIPNPNYELLEHIKKLGVVRLALEALKYGDFENINIQNEKLVYRRKTDNQTVFV
                     AFNLTDRVERIGFDTGCNAKLTDVLNGNEVIDVNGYYEIEMQPYQSRILVVNDGSFRV
                     DFNADNTDCRAAEIDEAPANEETSVKAVKTENCCESNSDNAENTTKEIKPGIYLHFKG
                     GEYEVINFATHSETGEKLVIYRNLHNTSEVWARPVEMFAEKVDVDGRKTDRFTFIG"
     gene            4312..4803
                     /locus_tag="PLS64_01770"
     CDS             4312..4803
                     /locus_tag="PLS64_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42593.1"
                     /translation="MLKMPENKTRKKLRITLCVLYLVEIVLCALPYFQYIDEKNVLHS
                     YSVFDIMSVWGLSGSNDAVFKYSLFMPIFFIIPIAGFFFCALDKERNMKNIVSIICCL
                     LGVLSIITMVSYTISLASVIALLLYILICFLTTLSIFARFTDKDATKAPVKQKNTDPN
                     REV"
     gene            complement(4965..5972)
                     /locus_tag="PLS64_01775"
     CDS             complement(4965..5972)
                     /locus_tag="PLS64_01775"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HRL42594.1"
                     /translation="MQHEITEKHNVFEENGEVMCAGWARTPVFEYNSEQSKTSGKHSE
                     RDCYFISNTEVSLYLSVENLGMEFAVKIAVADLKRGGVIYDCVVKKFVFSKNELPESG
                     SSGELLYTDKRLQLQLTNTPNGRFLKCDFIDFGGIKNLYFNINLKKTAGESLNEIAPF
                     ERDRKYFYLKRFVPKFVAGGIIRVGGMEYSLNETTTNAYFDWTRFSKPRKHNYQRLSS
                     DCFIDGKRFSLCLASRVGDNRYGNENCFFTDGHLVKLSQINVKSTGGKLDRPWYFKAG
                     YSAVDITFKPFTVKGEAMAAYMDKTTVIFGRLYGELKRVDMDKPLVLDNSQAHIVLTE
                     F"
     gene            6167..6532
                     /locus_tag="PLS64_01780"
     CDS             6167..6532
                     /locus_tag="PLS64_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42595.1"
                     /translation="MKKFVILLLICLCFLLCSCSADTSGYQNELTCTDWSAELDGGGK
                     ISLSFDGENASLIMMSRKDKSELCGKYVADDTTLVIFVPKLYRNYTFEYIPHGKKLDL
                     SYNGSKITLSRADNTQKTE"
     gene            6677..7183
                     /gene="ruvC"
                     /locus_tag="PLS64_01785"
     CDS             6677..7183
                     /gene="ruvC"
                     /locus_tag="PLS64_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRL42596.1"
                     /translation="MVVFGIDPGYAIVGWGAINFQSNTYKTLGYGAIETVAGTDFNKR
                     LEYIYDETYAILNRCRPDALAIEKLYFQTNQKTAINVAQARGVTLLAAQKLKIPIFEY
                     TPLQVKTAVTGYGKAKKPQVMEMTARLLKLKEIPKPDDTCDALAIAICHTQAAGSQLR
                     RVMYGKGL"
     gene            7186..7788
                     /gene="ruvA"
                     /locus_tag="PLS64_01790"
     CDS             7186..7788
                     /gene="ruvA"
                     /locus_tag="PLS64_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRL42597.1"
                     /translation="MLYSVRGKLIAIESNAAVVECGGVGYMCQTTMNTLKAVKLNTEV
                     TLYTYLNVREDAVDLFGFATKAELETFKNLISVSGVGPKAGLAVLSELSPEQVAMAIA
                     SDDLKTITRAQGIGKKIAQRIVLELKDKLAKAAKEDSSFAQAAQNSVNVSTGNVPKAI
                     EALGVLGYSPSDVSPVLATLDSALPVEQLISLTLKQMGRQ"
     gene            7801..>8831
                     /gene="ruvB"
                     /locus_tag="PLS64_01795"
     CDS             7801..>8831
                     /gene="ruvB"
                     /locus_tag="PLS64_01795"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRL42598.1"
                     /translation="MEFSDEFDFENRITDTSEIPEDTAESDNPLRPKTLSEYIGQEKA
                     KENLKVFIEAAKIRGETLDHVLLYGPPGLGKTTLSGIIANELGVSMRITSGPAIEKPG
                     DLAALLTNLNEGDVLFIDEIHRLSRSVEEILYPSMEDFAIDIITGKGQMAASYHLPLP
                     KFTLVGATTRAGQLTAPLRDRFGVVLRLELYTPEELAQIVERSAGILGIKIEHDGALE
                     IASRSRGTPRIANRLLKRVRDFAQVMSNGVITLETARTALDRLEIDELGLDRNDRRML
                     EAIVRFYNGGPVGLETLAAAIGEEAVTIEDVYEPYLLQIGFVSRTPRGRCITAEACKH
                     LGCEFPKGVV"
     assembly_gap    8832..8841
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8979..10328
                     /gene="glmM"
                     /locus_tag="PLS64_01800"
     CDS             8979..10328
                     /gene="glmM"
                     /locus_tag="PLS64_01800"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HRL42599.1"
                     /translation="MGRLFGTDGARGVANAELTPELAMNIGRAAAMVLISDEVEHPTI
                     LIGKDTRLSGDMLEGALIAGLCSVGANVHILGVVPTPAVAYLVGKYNADAGIMISASH
                     NPFEFNGIKIFSSDGCKLPDALENRIEEIVLDHVVPYASATDENIGRVTYDTEAADLY
                     IDHLVSAVDCDFEGMEIALDCSNGSSSRTAEKLFTKLGAKVHMLFDEPDGVNINRDCG
                     STHMSKLQKYVREHKLSCGLAFDGDADRCLAVDENGNLVDGDYLIAICANDMKQRGVL
                     KKNAVVGTIMTNMGFNKFCEENDMHFVSTKVGDRYVLEAMMQEGYNIGGEQSGHIILL
                     DYATTGDGQLSGAMILSIMKRTGEKLSQLAKIMERLPQVLINVKVSREGKLSFYTDRE
                     IKAEIERVSEILGDRGRILVRVSGTEPLVRVMLEGENLEEIQNLAEEAAQVVRERLA"
     gene            10328..11191
                     /locus_tag="PLS64_01805"
     CDS             10328..11191
                     /locus_tag="PLS64_01805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL42600.1"
                     /translation="MRLSDKWKDYELIDTSDGERLERWGDIILIRPDPQIIWSSEKKN
                     PLWYSAHARYHRSNRGGGSWEQYKKVPDRWTVNYGDLVFNIKPMGFKHTGVFPEQAVN
                     WDFAAEKIKNENRPLKILNLFGYTGCATLACMNAGATVCHVDASKGMVQWAKENAASS
                     GIADRPVRWLVDDCVKFVQREIRRGNRYDGIIMDPPSYGRGPGGEVWKLEEQLYSLVT
                     LCKQVLSDNPLFFILNSYTTGLSPAVMEYLLGVLLQKDFGGKVSSDEIGLKVSDTGLV
                     LPCGSTAIWEK"
     gene            11195..12040
                     /locus_tag="PLS64_01810"
     CDS             11195..12040
                     /locus_tag="PLS64_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRL42601.1"
                     /translation="MSFISLDNISFSYSDIESGQPYKNAVDGVSLNIEKGEFVALLGH
                     NGCGKSTVAKHLNAMLLPDKGKVYVDGDDTSDENKTYDIREKVGLVLQNPDNQLVASV
                     VEEDVAFGPENLGIPPKEIRQRVDDALKAVDMYDYREHAPFKLSGGQKQRIAIAGILA
                     MQPQCIVLDEPTAMLDPNGRDEVMTTLLKLNREKNMTVVLITHYMEEAVLADRVVVMD
                     AGKILTQGTPEEVFSQAELLKKHRLDVPQATELAYRLRGCGVKFDKLPLNAEQCVKML
                     EEVLS"
     gene            12037..12906
                     /locus_tag="PLS64_01815"
     CDS             12037..12906
                     /locus_tag="PLS64_01815"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRL42602.1"
                     /translation="MSKVLELKNLSYVYGTGTPFEKTAVNNLSLSIEKGEFIGIMGHT
                     GSGKSTLVQMLNGLMKPTSGQVLLDGEDIWANPKDIRKIRFKVGMVFQYPEYQLFEET
                     VAKDIAFGPTNMGKSGAELEKAVNDAARFTGLKDELLEKSPFDLSGGEKRRAAIAGVI
                     AMNPEVLVLDEPTAGLDPMGRDVLLSQIVQYHKERKNTVILVSHSMEDIARVADKIIV
                     MNKSNLVMFDKTKEVFSKGRELEKIGLRVPQITKIMLELREKGFNVPEGILTVDEAMD
                     CISSLLDKEGKIW"
     gene            12900..13712
                     /locus_tag="PLS64_01820"
     CDS             12900..13712
                     /locus_tag="PLS64_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRL42603.1"
                     /translation="MVRDVAFGQYFPANSVIHKCDPRAKLIMLVGFLVIIFCTFNYAS
                     LALVVLFTALFVAASKVPAKFYFKSMKVIIFIIIVTSILNVFYGSGDPLVQWGIFKIT
                     LNGINRAIFVSVRIICLILASSCLTFTTSPTELTDAIERLMKPLAKLHFPVHEIAMMM
                     SLALRFVPTLLEETDKITQAQKARGADMESGNIIQRVKAFIPILIPLFVSAFRRAYEL
                     ATAMECRCYRGGTGRTRMKTIHMAKRDYVSFVMVALLIGGVILCNIFLPAAV"
     gene            13682..14488
                     /gene="truA"
                     /locus_tag="PLS64_01825"
     CDS             13682..14488
                     /gene="truA"
                     /locus_tag="PLS64_01825"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRL42604.1"
                     /translation="MQHIPACGSLRNLLLTISYDGKKFHGWQIQQNAMTVQEAFQTAL
                     SKIIGNDFDVKGCSRTDSGVHANMYCISLKTEHPIKPERLKAALNRWLPLSIAVLDCK
                     EVAPDFHARYSCTSKEYIYKIWNSEVRNPFLDGYALHYRYKIDENLLDKAAQAYVGRH
                     DFTSFCTLDNREKGDMTRNVKAFSVTREGDLVTMKVEADGFLYNMVRIMVGTLLRIQQ
                     GKIAPDEIPSIIEKKNRKYSGPTAQACGLYLNRVNYENDNSSQKGVNADV"
     gene            14481..15776
                     /locus_tag="PLS64_01830"
     CDS             14481..15776
                     /locus_tag="PLS64_01830"
                     /inference="COORDINATES: protein motif:HMM:NF039834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5711 family protein"
                     /protein_id="HRL42605.1"
                     /translation="MFKKFKGHYSKRKTHKTDRDVMSYEDMPLDDYEQEKTEMSPQAV
                     KKIVLGACIAIAAGLIVFAFANRDKLTWENISNWWTYDVLGNAGHGYPVDIIGSEVSS
                     GNFAVSQGHAVYASDTSFVTLTNSGTEVARLQLTHSNPVMKNNENRFLTYGIGSSSYE
                     ISSFDETLYSGNAENKIYTGDVASNGTYCLVTEDNGYLTTLYAYSKNNNRIYKYSFSE
                     YYITSVAINKDGSGCVATGVSSDKGSAVTGVYVLDFSQEKPVSTYKISGDSVLDCKYL
                     TSDTAVLVGSQASYIVKKGEKNYKTVSYEDKTLSNYCFNTDTSTYTMALSRSGDGRSV
                     ALISYNSNGEAQYTINTEHKADSLSVYKGTVAILDGNVVYGYNQNGDLLYSTNSGTGS
                     KKIVLASDYTAYILSVNQIRFIDLDSVSSDDTAQTSKQE"
     gene            15782..16456
                     /locus_tag="PLS64_01835"
     CDS             15782..16456
                     /locus_tag="PLS64_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRL42606.1"
                     /translation="MVADIIIIAVFALLFFIDFKRGIAITILNVAGVALTGFLAYHIS
                     NFLASWVYTAFVQQTLTTNLQQMIDTQGINSAIANSFSALPNWVMGMLGFFLSIFGLD
                     SSVYTNDFQVPNSAAAAVSASVENLIQPVITGMFRLVIGVVISIIIFIIIKILVKKLA
                     RVFKIPVVKQINKLLGGVLGLAEAAILVFFAVNIFSGVLEFSNPEMLNNPMISGAVFK
                     FFSVAV"
     gene            16481..17098
                     /locus_tag="PLS64_01840"
     CDS             16481..17098
                     /locus_tag="PLS64_01840"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRL42607.1"
                     /translation="MEKEKDVWSFKTSDLVTIPNIITYVRFVLIIPFVYFFLAEQYIY
                     AAICIGLSGLSDCFDGMAARKLNQVTPLGKILDPIADKVTLFAVVICMVIYVPMVLPI
                     LVTLLVKDLIMLLGGADLIKKRLTPPSANWYGKVGTIIFYFSVCLIVFLKAFFAYENM
                     VLDLILLSVTAASMLYALYRYGKIYFAMIKEYNESLKKTADIKNK"
     gene            17201..19540
                     /locus_tag="PLS64_01845"
     CDS             17201..19540
                     /locus_tag="PLS64_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRL42608.1"
                     /translation="MLCTRCGKRPAVVFVSNNNSKDAPTAGYCLTCAKELGIKPVEDL
                     ISKMGISDEDLENVQDQMNTLMNSMGDGDMSQLMEQLGADNLAEQMGDFAKDGDGSED
                     DDFSHGAPAFPAFFNSMFGGQNNNATNQDNNKKGKKEKKEKNRKHINLYCEDLTRKAR
                     EGKIDRIVGRDSEIERVIQILSRRTKNNPCLIGEPGVGKTAIAEGLALRIAAGNVPQR
                     LKNKEIQLLDLTSLVAGTQFRGQFESRIKGLVSEIKENGNIILFIDEVHSLVGTGDNE
                     GTMNAANILKPALSRGEVQVIGATTFNEYRKYIEKDSALERRFQPVKVGEPTIAQSID
                     VIAGVKGYYEAHHRVIVDDDIVRKTVILSERYITDRFLPDKAIDLLDESCACAALRNK
                     NMERHDKLEDERQKLLVRKDALTNADEVNYEQLAEVNTSLARIDSDLKEIDPETLVSK
                     VTEEDIAKVIELWTGIPASRIKENELSKLADLENELKKKIIGQDEAVKALASAIRRSR
                     VQISPRRRPASFIFVGPTGVGKTELVKVLSKELFDTPETLIRLDMSEFMEKHSVSKII
                     GSPPGYVGYDEAGQVTEKVRRRPYSVLLFDEIEKAHPDVLNILLQILDEGRVTDAHGR
                     TVNFENTVIIMTSNAGSASKDSALGFGKTEEDASKEKVMKALSEFLRPEFIARVDEVI
                     VFNSLKKDDFVKIADLMLSEYVPTLEEKHIKFTFDEKVCQLLAEKSCNGASGARDLRN
                     TIRREVEEKIANAMIEAGAGGVTAMHLTAENGEPVLQSI"
BASE COUNT         5788 a         4116 c         4231 g         5488 t
ORIGIN      
        1 ttatacaaac ttttcaccga tatttcaagg aataaacttt ttatttatgc aatttctaca
       61 acaatccgaa acctataaaa acatgaagcc cccacacatt ttcgtgtagg ggcggttttc
      121 ttaaacaaag aaattgtcct tataattaat atctttctcc gacagcaaca atgacacttc
      181 tgcctgccat attaattcgt ctgtcataga gaataacatt tctgtcctca aggattacat
      241 cctgaggctc gttgcctgtg tttacataaa gcttccagtg gtatccttcg ggaaggtcgg
      301 gaagctcaac atttacataa tcccaataag cattgacgcc aaagtaaaca aactcgtcaa
      361 gcccctgttg tttaagactt acaccggcat acataacgcc aatcattctt gtatcatcgt
      421 aatacttggc actccacgca acatttgaat ggatgctgac aggcggataa ctgcatgttg
      481 ccttgcctct gctgtctgta ataatatgga atctctttct gaaagcaatc atatatttaa
      541 aaaattcaaa tacatcctta tgcttttctt ttcttgtcca gtcaagccat gagataatgt
      601 tgtcctgaca gtaagcattg ttgttgccga actgagtgtt gcagaattca tcacctgcaa
      661 ggaaaagagc aggtccctga ctgcacataa gggttgcaaa agcattcttt atcattttga
      721 tacggagctt gttgatattg taatcatttg tatcaccctc ggcaccgcag ttccagctgt
      781 tgttgtcatt agcaccgtca gtattgttcc agccgttttc aagattgtgc ttttcgttgt
      841 atgaatacat atcataaaga gtaaatccgt catgacaggt aagaaagttt accgacgcat
      901 tgtagcctct gtaggtaggg tcatacaaat ctcttgagcc tgtaattctc tgagccgcat
      961 cccatgcaag atggctgtcg cctttaagaa atcttctgag gtcgtcacgg tatcttccgt
     1021 tccattcagc ccatctgttc catgacggaa aactgccgac ctgataaagt ccgcctgcgt
     1081 cccatgcctc tgcaataagc tttactccgc cgagaatcgg atcaaacgca aggctcttga
     1141 gaagcggagg cttatccata ggagtaccgt cctcgtttct tcccaaaatt gacgcaagat
     1201 caaaacggaa accgtcaatg cggtattctg ttacccaata acgcagacag tcaagtataa
     1261 actgctgcac aatcggctga ttgcagttga gaacattgcc gcaaccgctg aaattgtaat
     1321 actttccgtc cggagtgagc atatagtaaa tgttgttgtc aatgcccttg aacgagaaat
     1381 acggtcccat ttcgttgccc tctgccgtgt ggttgaacac aacatcaagg ataacttcaa
     1441 ttccgttttc gtgaagctca cgaacaagct gtttaagctc tctgccttcg tgatgatgct
     1501 tgtggtcgct ttcatagctt gtgttcggag caaaaaaaca tacggtgtta tagccccagt
     1561 aatcgtagag ctgacgaccc tcataattac gatatgagcc catttcgtca aactcaaaaa
     1621 taggcatcat ttcaacggca ttgatgccca gttctttaag ataggggatt ttttcacgga
     1681 tacctgcgaa tgtgcctttg tttttaacgc ctgaggagcc gtcctgagta aagcctctta
     1741 catgcaattc ataaataata agctcttcaa acggaagctt cggctgagtg cagttgcccc
     1801 agtcatagtc gctgtttaca actcttgcct tataaacacc ctcatgctcc tgttttttac
     1861 cccagccgct ctgaccgatt acagcctttg cgtatgggtc aagaatgtat ttctttttat
     1921 caaaaataat tcctttttcg ggttcatacg gaccgtcaaa ggaatatgca tactcaaaat
     1981 caatctcatc aagaccgaac acgagcattg aataggtgtt gccgattttg taagaatccg
     2041 gaaatctgag ccttgcatac ggaaattttg cataaggtct gaataaaagc aatgtacact
     2101 caacggcata aaccgaatga atagtaaagt tcacaccgcc ggaaacggtt gttgcaccgt
     2161 ttaaaagaaa gttgccgggt ctgacatcga aaccacctac attatcggtt ggctttaaat
     2221 ccctcaacat aatattcaat ccccctaagt tactttcaaa aaaaacaaaa attcagtcaa
     2281 acacaacata tgtctatttt atcatattat gtgatttatt caacacacta tttgcaaaaa
     2341 tatagtcaaa tcttgtcatc acgattaaaa agcagtattt ttacacaaaa aaagaccgga
     2401 ataaaaaata tattcggtct ttttaatcat ttgcttacgc ttattgtcaa cctataaaag
     2461 taaatctgtc tgtttttctg ccgtcaacat caactttttc ggcaaacatt tcgacaggtc
     2521 ttgcccacac ttcacttgtg ttgtggaggt ttctgtaaat tacaagtttt tcacctgttt
     2581 cgctgtgggt tgcaaagttg ataacctcat attcgccacc cttgaaatgt aggtaaatac
     2641 ccggcttgat ttcttttgtc gtattttcgg cattgtccga attgctttca cagcaatttt
     2701 ctgttttaac agctttaact gaagtttctt catttgcagg agcttcgtca atttcagctg
     2761 ccctgcaatc tgtattgtct gcattgaaat caaccctgaa acttccgtca ttaacaacaa
     2821 gaatcctcga ctgatacggc tgcatttcaa tttcatagta accgtttaca tcaataactt
     2881 cgttgccgtt gagaacatct gtaagctttg cgttgcagcc tgtgtcaaaa ccgattcttt
     2941 caacacggtc tgtaaggttg aatgcaacaa aaacagtctg attgtcggtt tttcttctgt
     3001 atacaagctt ttcattctga atatttatgt tttcaaaatc gccgtacttg agagcttcga
     3061 gtgcaagtct tactacaccg agttttttga tatgctcaag aagttcatag ttcgggttcg
     3121 ggatggaatc aaggtcaaga cagggacgaa gctcataatc gctgtaggca gttctcatac
     3181 cctcaatgcc aaattcactt ccgtagtaaa ttgacggtac tccgggcatt gtatacatca
     3241 tcgtgtaaac attgttgaga tgatttttgt ctttaagcat acttgcaaca cggtttacat
     3301 cgtggttatc aacgaaatta tatgtgtaga tatttctgta aataccgccg ttagcaaact
     3361 gtctgttaag agaatgagca atttcaaaat agttgtggtc attatggctt gaataaatgc
     3421 ctttgtagca ttcgtagttt gtaaccgagt caagtacatc gggatttgcc caatgattgt
     3481 aatcgccgtg ggtaatctcg ccgtagagcc agaaatcgga ctttttctct ttgcaaacat
     3541 tttttaattt cttgaaaaat tcaatgtcaa tgcagtcagc cgcatcaagg cgaagtccgt
     3601 caatatcaaa ctcatcaatc caaaatcttg cacagtcaag aagatagttt actacctcgt
     3661 cattgtggag attaagcttt acaaggtcgt agtaacctgc ccagccctca tagctgaaac
     3721 cgtcgttata cgggctgttg ttccggaagt ttacattttc aaaccaagac gcatatcttg
     3781 aattttcacg gttctgctga acatctttga aggcaaagaa atctcttccc acatgattaa
     3841 aaacgccgtc aagcataatt ttgatgccgt tatcgtgaag tgttttgcaa atttttttga
     3901 aagattcgtt gtcaccgagc cgacagtcga tttttttata atcaatagta tcatagccgt
     3961 gttttgaact ttcaaaaaca gggttaaaaa caagtgcgtt taccgacatt gacttcatat
     4021 ggtcaatcca gccgtaaatt ttgtcaagtc tgtattcctg tttgaaatca ttctcctttg
     4081 gtgctccgca aaaaccgagc ggataaatat tataaattac ggattcattt atatatccca
     4141 ttatattatc tcctttttgc acagaataaa gcaatgtacg aatgcagtgt tttgtgtaca
     4201 ttagtcataa ttataacact aaaatttcta ttgtgcaact gaataatttg tgttacaatg
     4261 taacatattg atgatttaaa aatgatttaa aaaaaatctc ggaggaacaa aatgctcaaa
     4321 atgcccgaaa acaaaacaag aaaaaaatta agaatcacac tttgcgtgct ttatctcgtt
     4381 gaaatcgtac tctgtgcgtt gccgtatttc cagtacattg acgaaaaaaa tgtactccat
     4441 tcttattccg tatttgatat tatgtctgta tggggactca gcggttcaaa tgatgcggtt
     4501 tttaagtatt cgctctttat gccgattttc ttcattattc cgattgcagg atttttcttc
     4561 tgcgctcttg acaaagaacg caatatgaag aatatcgttt caatcatctg ctgtctgctc
     4621 ggtgtgcttt caattatcac aatggtaagc tatacaatca gccttgcttc tgtaattgca
     4681 cttcttttgt acattctcat ctgctttctc acaacgcttt caatctttgc aagatttacc
     4741 gacaaagatg ctacgaaagc tcctgttaag cagaaaaaca cagacccgaa cagggaagtg
     4801 taattttaca aattaaaagt tgaaaacgga aaattgaaaa tttcggtcgg gaacacagtt
     4861 tgcttaatgc aaagcattgt tttcccgact ttattttata aaattaaaac acggcagttc
     4921 attatctgaa atgattgccg tgtttatttt tgccgattta aattttaaaa ttccgtcaga
     4981 acaatgtgtg cctgagaatt atcgaggact aacggcttat ccatatcaac tctctttaat
     5041 tctccgtaga gtctgccgaa aattacggtt gttttgtcca tatatgccgc catagcctcg
     5101 ccttttacgg tgaacggctt gaatgtaatg tcaactgccg aatatcccgc cttaaagtac
     5161 cacggtctgt caagctttcc gcctgtgctt ttaacattta tctgcgacaa ttttacaaga
     5221 tgaccgtctg tgaaaaagca attttcgttg ccgtaacggt tgtcaccgac tctgcttgca
     5281 agacagaggg aaaagcgttt tccgtcaata aagcagtcgg agcttaatct ctgataatta
     5341 tgctttctcg gctttgaaaa gcgtgtccag tcaaagtaag cgtttgtggt tgtttcgttt
     5401 aaagaatatt ccataccgcc aacacggata attccgcctg cgacaaattt gggaacaaag
     5461 cgtttgagat aaaaatattt tctgtctctc tcaaacggtg caatttcgtt aagactttcg
     5521 cccgccgtct ttttgagatt aatgttaaag taaagatttt ttatgccgcc aaagtcgata
     5581 aaatcacatt ttaaaaatct tccgttcggt gtgtttgtca actgcagctg aagccttttg
     5641 tccgtgtaca aaagctctcc cgaactgccc gattcgggaa gctcgttctt gctgaaaaca
     5701 aatttcttta caacacaatc ataaatcaca ccgcctcttt taaggtcggc aaccgcaatt
     5761 tttaccgcaa attccatgcc taaattttca accgaaagat agagcgaaac ctctgtgttg
     5821 cttatgaaat agcaatccct ttcgctgtgt tttccgcttg ttttgctctg ttcgctgtta
     5881 tattcaaaaa caggcgttct tgcccagcct gcacacataa cctctccgtt ttcttcaaaa
     5941 acattatgct tttcggttat ctcatgttgc aaaacaatcc ctccgtgttc tcgtttttaa
     6001 aattttccat aaaatcacac catacaatat acacaaaata attatgcttt tcaagaatta
     6061 taacaaaact gtaaatttcg ttttctgccg tcaacggtat atgtactaat tttctgattt
     6121 catgaataat ctgtaataat atcatatacc tgtgagggcg gtttttatga agaaatttgt
     6181 cattttgctt ttaatctgct tatgttttct gctttgttca tgcagtgccg acacatcggg
     6241 atatcaaaac gagcttacct gcacagactg gagtgcagag cttgacggcg gcggaaagat
     6301 aagcctttcg tttgacggtg aaaatgcgtc tcttattatg atgagcagaa aagacaagtc
     6361 ggagctttgc ggaaaatatg ttgccgatga tacaacgctt gtcatatttg tgccgaaact
     6421 ttaccgaaac tacacctttg aatatatccc ccacggaaaa aagcttgatt tgtcatacaa
     6481 cggttcaaaa atcacgctca gccgtgccga taacacgcaa aaaaccgagt aaatcagccg
     6541 ttcaaagttt cttatgtaca ttttgagcgg aatacgcaga ttaacgaata attttattga
     6601 taaataaagt tgtgtgtgtt ataatgtttt aagctatgtt attatgccat ttttggattt
     6661 tttcggaggt attgttttgg tagtgtttgg aattgatccc ggatatgcca ttgtaggttg
     6721 gggagcaatc aattttcaat cgaatacata taaaactctc ggttacggtg caattgaaac
     6781 cgttgccgga actgatttta acaaacggct tgagtacatc tatgacgaaa cctatgctat
     6841 tctcaacaga tgtcgccccg acgcccttgc aatcgaaaaa ctttactttc agaccaatca
     6901 gaaaacggct atcaatgttg ctcaggcaag gggagtaacc ctgcttgcgg cacaaaaact
     6961 taaaatacca atctttgagt acacgcctct tcaggtcaaa acggctgtaa cgggatacgg
     7021 caaggctaaa aaaccacagg ttatggaaat gaccgcaagg cttttaaagc tgaaagaaat
     7081 tccgaaaccc gatgatacct gtgacgcact tgcaattgca atctgtcaca cacaggcggc
     7141 aggctcacag ctcagaagag taatgtacgg aaagggactt taaatatgct ttattccgta
     7201 agaggaaaac ttattgctat agaatcaaat gcggcggtag tagagtgcgg cggtgtcggc
     7261 tatatgtgcc agacaacaat gaacacactc aaagccgtaa agcttaacac agaggtaacg
     7321 ctctacacct accttaatgt aagggaagat gcggttgacc ttttcggctt tgcaacaaag
     7381 gcagagcttg aaacctttaa aaacctcatc agcgtaagcg gtgtaggtcc aaaggcagga
     7441 cttgcggttt tgtcggagct cagtcccgaa caggttgcaa tggcaattgc ctccgatgac
     7501 ctcaaaacaa ttacaagagc acagggaata ggcaaaaaaa ttgcacagag aattgttctt
     7561 gaactcaagg acaagcttgc aaaagcggct aaagaggaca gttcctttgc acaggcggca
     7621 cagaacagcg tgaatgtttc aacgggaaat gttccgaaag caatcgaggc actcggagtt
     7681 ttgggttatt ctccttcgga tgtttcgccc gtactcgcaa cactcgacag cgccttgcct
     7741 gttgaacagc ttatttcgct cacattaaaa cagatgggaa gacagtaaca tatgagtaaa
     7801 atggaattct cggatgaatt tgattttgaa aacagaataa ccgatacttc cgaaattccc
     7861 gaagatacgg cagagagcga caaccctctg cgaccgaaaa cactttcgga atacatagga
     7921 caggaaaagg caaaggaaaa cctcaaggtc tttatcgagg cggcaaaaat cagaggcgaa
     7981 acccttgacc atgtactgct ctacggccct ccgggactcg gtaaaacaac tctttcggga
     8041 atcattgcga atgaactcgg tgtgtcaatg agaataacct caggccctgc aattgaaaag
     8101 ccgggcgacc ttgcggcatt gcttaccaac cttaacgagg gcgatgtgct ttttattgac
     8161 gaaattcaca gactgagccg ttccgttgaa gaaatacttt acccttcaat ggaggacttt
     8221 gcaattgata tcatcacggg taaaggtcag atggcggctt cgtatcatct gccgttgcca
     8281 aaatttacac ttgtcggagc aaccacaaga gcaggtcagc tgaccgctcc tttgcgtgac
     8341 cgcttcggtg tggtgttaag acttgagctc tacactcccg aggagcttgc acagattgtt
     8401 gaaagaagtg ccggaatcct cggaataaaa attgaacatg acggtgcgct tgaaattgcg
     8461 tcccgttcaa ggggaacacc acgaattgca aaccgacttt taaagcgtgt gcgtgacttt
     8521 gcacaggtta tgtcaaacgg cgtcatcaca cttgaaaccg caaggacagc acttgacaga
     8581 ctcgaaattg acgaactcgg tcttgaccgc aacgacagaa gaatgcttga ggcaatcgtt
     8641 cgtttttaca acggcggtcc tgtcggactt gaaacacttg cggcggcaat cggtgaagag
     8701 gctgtaacaa tcgaggatgt ctatgaaccg tatcttttgc agataggttt tgtcagcaga
     8761 acaccgaggg gcagatgcat aactgccgag gcttgcaagc atctcggctg tgaatttcct
     8821 aagggcgttg tnnnnnnnnn nggtgacagg gagtcataaa tcagtcacaa tatgagtgta
     8881 taattcaaca gagtgtaagg tttttacttg ggttataccg aaaattgcag ttttgaaaat
     8941 ttacaatcta caaattattt aacggaggaa tatatataat gggaagatta ttcggtacag
     9001 acggtgcaag aggtgttgca aacgcagagc ttacacccga acttgcaatg aatataggca
     9061 gagcggcggc aatggttctt atcagcgatg aggttgaaca ccccacaatt ttaatcggta
     9121 aggacacaag actttcgggc gatatgctcg agggcgcact tattgcagga ctttgctcgg
     9181 taggtgcaaa tgttcacatt ctcggggtag ttcctactcc tgcggttgct tatcttgtag
     9241 gcaaatacaa tgccgatgca ggtattatga tttccgcatc acacaacccg tttgaattta
     9301 acggtatcaa aattttcagt tctgacggat gcaagctccc cgatgccctc gaaaacagaa
     9361 ttgaagaaat cgtacttgac catgttgttc cgtatgcatc cgcaacagat gaaaatatcg
     9421 gcagagtaac atatgataca gaggcggctg atttgtatat tgaccacctt gtttcggcag
     9481 ttgactgcga ctttgagggt atggaaatcg cacttgactg ctcaaacggt tcttcttcaa
     9541 gaacagccga aaagcttttc acaaagcttg gtgcaaaggt tcatatgctt tttgacgagc
     9601 ctgacggtgt gaacatcaac cgtgactgcg gttcaactca tatgagcaaa ttgcagaagt
     9661 atgttcgtga acacaagctt tcatgcggtc ttgcatttga cggtgacgcc gacagatgtc
     9721 ttgcggttga cgaaaacgga aaccttgttg acggcgatta tcttattgca atctgtgcaa
     9781 atgatatgaa gcagagaggc gtactcaaaa agaatgccgt tgtcggaaca attatgacaa
     9841 acatgggctt taacaagttc tgcgaagaaa acgatatgca ctttgtttca acaaaggttg
     9901 gcgacagata tgttcttgag gctatgatgc aggagggcta caacatcggc ggcgagcaga
     9961 gcggtcacat cattttgctt gactatgcaa caacaggcga cggtcagctt tcaggtgcaa
    10021 tgattctcag cattatgaag agaacaggcg aaaagctcag tcagcttgca aagattatgg
    10081 agcgtttgcc gcaggtgctc attaatgtaa aggtcagcag agagggcaag ctctcattct
    10141 atacagacag ggaaatcaag gctgaaatcg aaagagttag cgaaatcctc ggtgacagag
    10201 gcagaattct tgttcgtgta tcgggaacag aacctctcgt aagagtaatg ctcgagggcg
    10261 aaaacctcga agaaattcag aaccttgcgg aagaagccgc acaggtagta agggaaagac
    10321 ttgcataatg cgattatctg ataaatggaa agattatgaa cttatagata cctctgacgg
    10381 ggaaagactt gaaagatggg gagatataat ccttatccgt cccgatccgc agattatctg
    10441 gtcaagcgaa aagaaaaatc ctctttggta ttcggcacat gcccgttacc accgctcaaa
    10501 tcgtggcgga ggaagttggg agcagtacaa aaaagttccc gaccgctgga ctgtaaatta
    10561 cggcgacctc gttttcaaca tcaagcctat gggattcaag cacacgggtg tgtttcccga
    10621 acaggcagtt aactgggatt ttgccgcaga aaaaataaaa aacgaaaaca gaccgcttaa
    10681 aattctcaac cttttcggtt acacaggatg tgcaactctt gcgtgtatga acgcaggtgc
    10741 aaccgtttgc catgttgacg catcaaaggg catggttcag tgggcaaagg aaaatgccgc
    10801 atcaagcggt attgccgaca gacctgtccg ttggctcgtt gacgattgcg taaaatttgt
    10861 acagcgtgaa atccgccgtg gcaacaggta tgatggaatc attatggatc ctccgtcata
    10921 cggcagaggt ccgggcggtg aggtctggaa acttgaagaa cagctttatt cactcgtaac
    10981 cctgtgcaaa caggttctct cagacaatcc gctgtttttc attctcaatt cctacacaac
    11041 gggactttct cccgctgtaa tggagtattt gctcggagtt cttttgcaga aggacttcgg
    11101 cggaaaagta tcaagcgatg aaataggact gaaggtgagc gacactggac ttgtattgcc
    11161 gtgcggctca accgcaatat gggagaaata ataaatgagc tttatttcac ttgacaacat
    11221 cagcttttca tattccgaca ttgaaagcgg tcagccgtac aaaaatgcgg ttgacggtgt
    11281 ttctctcaat atcgaaaagg gtgaatttgt tgcccttctc ggtcacaacg gatgcggaaa
    11341 gtcaactgtt gcaaaacatc tcaacgcaat gctattgccg gataagggca aagtgtatgt
    11401 tgacggagat gacacatcag atgaaaacaa gacatatgac ataagagaaa aagtgggact
    11461 tgtacttcaa aatcccgaca accagcttgt tgcaagcgtt gttgaggaag atgttgcttt
    11521 cggccccgaa aacctcggaa taccgccaaa ggagatccga cagagggttg acgatgccct
    11581 caaagccgtt gatatgtacg attaccgtga acacgctccg ttcaagcttt ctggcggtca
    11641 gaaacagcgt attgcaattg ccggaattct tgcaatgcag ccgcaatgca tagttcttga
    11701 cgaacccacg gcaatgcttg accctaacgg ccgtgacgag gtaatgacaa cgcttttaaa
    11761 actcaaccgt gaaaagaata tgacggttgt cctcatcaca cactatatgg aagaggcagt
    11821 ccttgccgac agagttgtcg ttatggacgc aggaaaaatc ctcactcagg gtacacccga
    11881 agaagttttt tcacaggcgg agctgctcaa aaagcaccgt cttgatgttc cgcaggcaac
    11941 agagcttgca tacagactca gggggtgcgg agtgaaattt gacaaacttc ctctcaatgc
    12001 agagcaatgt gtaaaaatgc ttgaggaggt gctgtcatga gcaaggtgct tgaactgaaa
    12061 aatttaagct atgtttacgg aacaggcact ccgtttgaga aaaccgctgt caataatttg
    12121 agcctttcaa ttgaaaaagg cgagtttatc ggcattatgg gacacacggg ctcgggcaag
    12181 tcaacacttg ttcaaatgct caacggactt atgaaaccaa cttcgggaca ggttcttctt
    12241 gacggcgagg atatctgggc aaatcccaag gatatcagaa aaatccgttt caaagtcgga
    12301 atggtatttc agtatcccga atatcagctt tttgaagaaa ccgttgccaa ggacatcgca
    12361 ttcggcccta cgaatatggg caagagcggt gcagagcttg aaaaagcggt aaatgacgcc
    12421 gcaagattta ccggacttaa agatgaactt cttgaaaaat ctccgttcga cctttcgggc
    12481 ggtgaaaaga gaagggcggc aattgcaggc gttatagcaa tgaatcccga ggttctcgtt
    12541 cttgacgaac cgaccgcagg tcttgatcct atgggcagag atgtgctgtt gagccagatt
    12601 gtgcagtatc acaaggaacg caaaaacacc gttatccttg tatcccacag catggaagac
    12661 attgcaaggg ttgccgataa gataatcgtt atgaacaagt caaacctcgt tatgtttgat
    12721 aagacaaaag aagttttctc aaagggacga gagcttgaaa aaataggact tcgagtaccg
    12781 cagataacaa agattatgct tgaactgcgt gaaaaaggtt ttaatgttcc cgaaggaata
    12841 cttactgttg atgaggcaat ggactgtatt tcctcactgc ttgacaagga gggtaaaata
    12901 tggtaagaga tgttgcgttc ggtcagtatt ttcctgcgaa cagcgtaatc cacaaatgtg
    12961 atccccgtgc aaagctgatt atgcttgtcg gatttctcgt aattattttc tgtactttta
    13021 attatgcttc gcttgcgctt gttgtgcttt ttaccgcact ttttgttgcg gcaagcaagg
    13081 tgcctgcaaa gttctatttc aaaagcatga aagtaattat atttattata atagtaacct
    13141 caattctcaa tgtgttctac ggttcgggtg atccgcttgt acaatgggga attttcaaaa
    13201 ttaccttaaa cggcattaac agagcaattt ttgtttcggt aagaatcatc tgtcttatcc
    13261 ttgcaagctc ctgtcttaca ttcacaacct ctccgacaga gcttacggac gcaatcgaaa
    13321 gactgatgaa gccgcttgca aaactgcact ttcccgttca cgagattgca atgatgatgt
    13381 cgcttgcttt aagatttgtt cccacacttc ttgaagaaac cgacaaaatt actcaggctc
    13441 aaaaggcacg aggtgccgat atggagtcgg gtaatattat tcagagggta aaggcgttta
    13501 tccctattct tattccgctt tttgtttcgg cattcagaag agcctatgag cttgcaacag
    13561 ctatggagtg ccgttgctac cgtggcggaa caggaagaac acgaatgaaa actattcata
    13621 tggcaaagcg tgactatgta tcttttgtaa tggtcgcact tctaatcggc ggagtgattt
    13681 tatgcaacat attcctgcct gcggcagttt aagaaatctt cttctcacaa tttcatatga
    13741 cggaaaaaag ttccacggct ggcagattca gcagaacgca atgacggttc aggaggcttt
    13801 tcaaactgct ctttcaaaga ttatcggcaa tgactttgat gtaaagggat gcagtcgcac
    13861 agacagcgga gttcacgcaa atatgtactg catcagcctt aaaacagagc atcccataaa
    13921 gcccgaaagg ctcaaggcgg cgcttaaccg ctggctgccg ctaagcattg cggttcttga
    13981 ctgcaaagag gttgcacccg attttcacgc ccgctattcc tgcacaagca aggaatatat
    14041 ctacaaaatc tggaacagcg aggtgcgaaa tccgtttctt gacggatacg cacttcatta
    14101 cagatacaaa attgacgaaa atctgctcga caaagccgct caggcatatg tcggcagaca
    14161 cgatttcact tccttttgca cgcttgataa ccgtgaaaaa ggggatatga ccagaaatgt
    14221 aaaagccttt tcggtaaccc gtgagggcga ccttgttaca atgaaggttg aggctgacgg
    14281 ctttttgtac aatatggttc gtattatggt gggaacactt ctcaggattc aacagggtaa
    14341 aattgctccc gatgaaattc cctccattat agaaaagaaa aacagaaaat attcgggtcc
    14401 caccgcacag gcttgcggat tgtacctgaa tcgtgtgaat tatgaaaatg acaacagttc
    14461 acaaaaagga gtgaacgctg atgtttaaaa aatttaaagg acattattca aaacgaaaga
    14521 cacataaaac cgacagagat gtaatgagct atgaggatat gcctcttgac gattatgaac
    14581 aggaaaaaac cgaaatgtcg ccgcaggctg tcaagaaaat cgtactcggt gcgtgtattg
    14641 ccattgctgc aggacttata gtttttgcct ttgcaaaccg tgacaagctc acatgggaga
    14701 acatctcaaa ctggtggact tatgatgtac tcggaaatgc cggtcacggc tatcccgttg
    14761 acattatcgg ttccgaggtt tcatcgggca actttgcagt gtcacaggga catgccgtgt
    14821 atgcgtcgga cacctcgttc gtaaccctta caaattcggg tacagaggtt gcacgacttc
    14881 agcttacaca ttcaaatccc gtaatgaaaa acaatgaaaa cagatttctc acatacggaa
    14941 tcggcagttc aagctatgag ataagcagtt ttgacgaaac gctttactca ggcaatgccg
    15001 aaaataaaat atacacaggt gatgttgcct caaacggtac atactgtctt gttaccgagg
    15061 acaacggtta tcttacaacg ctgtatgcct acagcaaaaa taataacaga atttacaaat
    15121 attctttttc ggagtattat ataacatcgg tagcaattaa caaggacggc tccggttgcg
    15181 ttgccacagg tgtgtcaagt gataagggtt cagcggtaac gggcgtttat gtgcttgatt
    15241 tttcacagga aaagcctgtt tcaacctaca aaatttcggg tgacagcgtg cttgattgca
    15301 agtatctgac ctcagatacg gcggttcttg tgggctcaca ggcaagctat attgttaaaa
    15361 aaggtgaaaa gaattacaaa acagtaagct atgaggacaa aactctttca aattactgct
    15421 ttaacacaga tacatcaacc tatacaatgg ctctttcaag gagcggtgac ggaagaagtg
    15481 ttgctctcat aagctataac agtaacggcg aggcacagta cacaataaat accgaacaca
    15541 aagccgattc gctttctgtc tacaagggta cggttgcaat ccttgacgga aatgttgttt
    15601 acggttataa tcaaaatggt gatttgcttt attcgacaaa ttcgggaacc ggttcaaaaa
    15661 agattgtgct tgcctcggac tacaccgctt acattctcag cgtaaatcaa attaggttta
    15721 ttgaccttga cagtgtatcg agcgacgata ccgctcaaac ttcaaaacag gagtaataaa
    15781 tttggttgca gatataatta ttattgccgt gtttgcgctt ttgtttttta ttgatttcaa
    15841 gcgtggcata gcaattacaa ttctcaatgt tgcaggtgtg gctctcacgg gctttcttgc
    15901 atatcacatt tcaaattttc ttgcatcgtg ggtttatacg gcatttgttc agcagacctt
    15961 aacaacaaat ctgcaacaaa tgattgacac tcagggcatc aactcagcca ttgcaaacag
    16021 tttttcggct ttgccaaact gggttatggg aatgctcgga ttcttccttt caattttcgg
    16081 attggattca agtgtctata caaacgattt tcaggttccg aactctgccg cagcggcggt
    16141 gtcagcttcg gtggaaaatc ttattcagcc ggttatcaca ggaatgttcc gcttggttat
    16201 cggtgtagtt atatcaataa ttatctttat aattataaaa attcttgtta aaaaacttgc
    16261 aagagtgttt aaaatacccg tggtgaaaca gattaataag cttctcggcg gtgtgttggg
    16321 acttgcggag gcggcaatcc ttgtattttt tgccgttaat atcttttcgg gagtgcttga
    16381 gttttcaaat cccgaaatgc ttaataaccc gatgatttcg ggtgctgttt ttaaattttt
    16441 cagcgttgca gtttgaatga agaaacagga gggaacagta atggaaaaag aaaaagatgt
    16501 gtggagtttt aaaacatccg atttggttac tatacctaat attataactt atgtaagatt
    16561 tgtgcttata atcccgtttg tatatttttt ccttgcggaa cagtatatat atgcggcaat
    16621 atgtataggt ttgtcgggac tttccgattg ctttgacgga atggctgcca gaaaactcaa
    16681 tcaggtaact cctctcggaa aaatccttga tcccattgca gacaaggtaa cgctttttgc
    16741 ggtcgttatc tgtatggtga tttatgtgcc tatggttctt ccgattcttg taacacttct
    16801 ggtcaaagac cttattatgc tcctcggagg tgccgattta atcaaaaaaa gactcactcc
    16861 gccttctgca aactggtacg gaaaggtcgg aacaattatt ttctactttt ccgtatgtct
    16921 tatcgtattt ctcaaggcct tttttgccta cgaaaatatg gtgcttgatc tgatattgct
    16981 ttcggttaca gccgcatcca tgctatacgc tttgtatcgt tacggcaaaa tctattttgc
    17041 aatgattaag gagtacaacg aatcacttaa aaagacagct gacattaaaa ataaataaat
    17101 tccgacaaat aaatattgaa ttatttgtta aacagttgta taattcttat attaccatga
    17161 cagcaaatgt gcaatttggt gtcctaaaaa ggagtatgct atgctttgta caagatgcgg
    17221 aaaacggcct gcggttgttt tcgtttcaaa taataattcc aaagacgccc cgacggcagg
    17281 ttattgcctt acctgtgcaa aagagcttgg aatcaagccg gttgaggatc ttatcagcaa
    17341 aatgggtatc tcggatgagg accttgaaaa tgttcaggat cagatgaaca ccctcatgaa
    17401 cagtatgggt gacggcgata tgtcgcagct tatggaacag cttggtgcgg ataaccttgc
    17461 cgagcagatg ggcgattttg ctaaagacgg tgacggcagc gaggatgatg acttctctca
    17521 cggtgcacct gcattccctg catttttcaa cagtatgttc ggcggacaga acaacaatgc
    17581 gacaaatcag gataataata aaaagggcaa aaaagagaaa aaagaaaaga acagaaagca
    17641 catcaatctt tactgtgaag acctcacacg caaggcgaga gagggcaaaa tcgaccgcat
    17701 tgtcggcaga gacagcgaga ttgagcgtgt aattcagatt ctttcaagaa gaacaaagaa
    17761 caatccttgc cttatcggtg aacccggtgt aggtaaaacc gctattgcag aggggcttgc
    17821 tctccgcatt gccgcaggaa atgttcctca aagacttaaa aacaaggaaa ttcagcttct
    17881 tgacctcacc tcacttgttg caggtactca gttcagaggt cagtttgaaa gcagaatcaa
    17941 aggacttgtt tccgaaatca aggagaacgg caacataatt ctctttattg atgaggttca
    18001 cagccttgtc ggcacaggtg acaacgaggg tacaatgaat gccgcaaata tcctcaaacc
    18061 ggctctttca aggggcgagg ttcaggtcat cggtgctaca acattcaacg aatacagaaa
    18121 atatatcgaa aaggattctg ctcttgaaag aagatttcag cccgtaaagg tcggcgagcc
    18181 tacaattgct cagtcaattg atgttatcgc aggcgtaaaa ggctattatg aggcacatca
    18241 ccgtgtaatt gttgacgatg atatcgttcg caagacggtt atcctttccg aaagatacat
    18301 aacagaccgt tttcttcccg acaaagcaat cgaccttctt gatgaaagct gtgcctgtgc
    18361 cgctctccgc aataaaaaca tggagcgtca cgataagctt gaggacgaaa gacagaagct
    18421 tcttgtcaga aaggacgctc ttacaaatgc cgatgaggta aactatgaac agcttgccga
    18481 ggtcaacaca agccttgcaa gaattgacag cgaccttaaa gaaattgatc ccgaaactct
    18541 cgtttcaaag gttacagagg aggatatcgc caaggtcatc gaactctgga caggcatccc
    18601 cgcatcaaga atcaaggaaa acgagctttc aaagcttgcc gaccttgaga acgagcttaa
    18661 aaagaaaatt atcggtcagg acgaggctgt aaaggctctt gcatctgcaa tcagaagaag
    18721 cagagttcag atttcacctc gccgcagacc tgcatcgttc atctttgtag gtcctacagg
    18781 tgtcggtaaa acagagcttg taaaggttct cagcaaggaa cttttcgata cccccgaaac
    18841 tcttatcaga cttgatatga gtgaatttat ggagaaacat tcggtttcta aaattatcgg
    18901 ttcaccgccc ggatatgtcg gttacgatga ggcaggacag gtaacggaga aagttcgccg
    18961 cagaccttat tcggttctcc tctttgatga aatcgaaaag gctcatcccg atgttctcaa
    19021 tattttgctc caaattcttg acgagggcag agttacagac gctcacggca gaacagtaaa
    19081 ctttgaaaat actgttatca ttatgacatc aaatgccggt tctgcatcaa aggacagcgc
    19141 attaggcttt ggcaagactg aagaagatgc ctcaaaagaa aaggttatga aggcactttc
    19201 cgagttcctc cgtcccgaat ttatcgcaag agttgacgag gttatcgtat tcaacagcct
    19261 taaaaaggac gattttgtaa agattgcaga ccttatgctc tctgagtatg taccgactct
    19321 tgaggaaaag cacatcaagt tcacatttga cgaaaaggtt tgtcagctcc ttgcagaaaa
    19381 gtcatgcaac ggtgcaagcg gtgctcgtga cctcagaaac acaattcgca gagaggttga
    19441 ggagaaaatt gcaaacgcaa tgattgaggc aggcgcaggc ggtgttactg caatgcacct
    19501 tacagccgaa aacggtgagc ctgttcttca gtcaatctga tttttacggt aaaagttaac
    19561 acataaaatt tccgcatatc gggcagtcat cacgattgcc cgattttttt gttacaatat
    19621 aattctatta aag
//
LOCUS       DAOMVE010000016        19010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_530_length_19010_cov_3.261145, whole genome shotgun sequence.
ACCESSION   DAOMVE010000016 DAOMVE010000000
VERSION     DAOMVE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 19010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19010
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_530_length_19010_cov_3.261145"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..1339)
                     /locus_tag="PLS64_01850"
     CDS             complement(134..1339)
                     /locus_tag="PLS64_01850"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRL42609.1"
                     /translation="MSLIVQKFGGSSVANAERVMNVASIVTDTYAKGNDVVVVVSAQG
                     DTTDDLIEKANEINPKASKREKDMLLAAGEQISISLLAMAIEKLGYHAVSLLGWQAGF
                     NTTSAHTSARIRKVEPDRIKKELDKKNIVIVAGFQGISKYGDITTLGRGGSDTSAVAI
                     AAAMHADLCQIFTDVEGVYTADPRKVKNAKKLQEISYDEMLELATLGAQVLNNRSVEM
                     AKKYNIELEVLSSMTKASGTIVKEKTKMEKMLISGVAKDTDVARISVMGVPNIPGLAF
                     KMFSKLSAKDINVDIILQSIGHDGKKDISFTVAKNRGEEAVALMKEYTENLGAEEILY
                     DDKVAKISIVGAGMESHAGVATKMFEALYDAQVNIQMISTSEIKVSVLIDSADADKAV
                     SAIHSKFFD"
     gene            complement(1364..2599)
                     /locus_tag="PLS64_01855"
     CDS             complement(1364..2599)
                     /locus_tag="PLS64_01855"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRL42610.1"
                     /translation="MVSIAIMGHGVVGSGVAEIFTTHKQKLYASIGEEVYVKKILDLR
                     EFPDSPLADRFTKNFEDIINDLEIRVVVEVMGGLNPAYDFVKRCLKAGKSVVTSNKEL
                     VAAHGAELLEISKETNTNFLFEASVGGGIPIIRPMSQCLVANNVDEIAGILNGTTNFI
                     LTKMIEDGMQFDDALKLAQELGFAERNPAADIEGHDACRKICILASLAYGKHVYPDSV
                     HTEGITKITLEDVRYAEAFHCVIKLIGSVKRLADGKIDIIVAPMLVPNKSQLANIDYE
                     FNGIMVRGDCTGDVVFYGKGAGKLPTASAVVADVIDCCKHLKTRKFLYWADGNGKNIL
                     PWEESSRAMYVRANGSNVAEKAEKLFGEVTVINKADAPADEKAFVVKAMPYNEFAEKI
                     AQLEADGVSVLSSIRVADL"
     gene            complement(2645..5032)
                     /locus_tag="PLS64_01860"
     CDS             complement(2645..5032)
                     /locus_tag="PLS64_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRL42611.1"
                     /translation="MRNKRETDISQYKKDIQQNELSEKADGGVKRFFRGFGKFLITVC
                     SVCLVALLITGISLAVYIFTIASEPTGIDLKAKSMNQTSRIYIQNEDTKEFKEYQKLY
                     DTENRIWVDNQDIPQAMKDAVVAIEDKRFFDHNGVDWGRTLSAVANLATGSDSYGGST
                     ITQQLIKNITDDNEVSITRKLREITKALKLEQEYTKDQILEAYLNVVNFGNNCQGVES
                     AAQLYFGKSIKDCSIAECAAIAGITQNPSRWNPLVFPENNKERREIVLNEMYDQKKIS
                     KDEFDAAMKESATMKFVGWQASDDDDDDDEADVQNWYIDQVFRDLQKDIAEYYNISES
                     AASSKLYTEGLKIYCAMDENAQTYLENAALNIDKSNDSDLQIASTIMGYDGRVIATVG
                     SSTKKEGALSWDRSYNSVLQPGSSIKPVVVYPYAIESKKLYFSSMVLDEPLDNYRTNE
                     SGQLVSGPNNAYGYYNGNMSLPDAIEWSSNATAAQTMELIGGPSVAYQQVVTKMGFQN
                     LTEQDAQNTGALSIGGMNGGVTVREMAAAYTYLGNGGLYYEPYTYYYVTDSEDNIIID
                     NRDAVPKQAYSAETAGIMNRLLHYNVTNSAHTNAHYAQISGWDIIGKTGTTDDDKDSW
                     FCGCSPYAVMATWCGFDKPKTISYSGRTTATKFFANVMGKYLKGKENKEYKISDNLIE
                     ATYNPTTGLIVSTDNISGRYVGYYTEDNMPSSGGSYYSGNYEYGSDVSDSSSYYNPNY
                     GGDNSGSNYGGDTSNSGGDNSGGDTSGGGESSGGGESSGGGESSGGGEEAPPAAE"
     gene            5357..5686
                     /locus_tag="PLS64_01865"
     CDS             5357..5686
                     /locus_tag="PLS64_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42612.1"
                     /translation="MKKIVGLTGVILIACMMISSLFAPVNSAENTSATGNYSSSQNNG
                     TTEGDYIIKAEKGYIVVYKKGEDKAYLTTDCRVSNLPKGDALYLEKGIEIKGKENLMR
                     ALEDYCS"
     gene            5897..6847
                     /gene="fabK"
                     /locus_tag="PLS64_01870"
     CDS             5897..6847
                     /gene="fabK"
                     /locus_tag="PLS64_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HRL42613.1"
                     /translation="MISTVIDQSLGLEFPIFQGGMAWVADASLAAGVSNAGGLGIIAA
                     MNSNGEQLREEIRKCKTMTDKIFGVNIMLMSPFAEEVSDVVVEEGIKVITTGAGNPGK
                     YMAKWLEAGIKVIPVVPSVALARKMEKDGAFAVIAEGGESGGHVGDLTTMALVPQVVD
                     AVNIPVIAAGGIADGRQIAAAFMLGAQGVQVGTRFLVAKECTISQEYKNKILKARDID
                     TVVTGKRLGHPVRSIRNSFTREYTKAEYDSANVSDEELENMGLGRLRRAVREGDVSQG
                     TVLAGQVASMVKKEQPAREMIEEMFSQAEEVLNGATKWVK"
     gene            6835..7737
                     /gene="fabD"
                     /locus_tag="PLS64_01875"
     CDS             6835..7737
                     /gene="fabD"
                     /locus_tag="PLS64_01875"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRL42614.1"
                     /translation="MGKIAFIFSGQGAQYSGMGKDLYDNSPAAKAVYEMADSIRENTS
                     KQCFEGTMEELSKTVNTQPCIFTADLAAARALVEKGITPDCVAGFSLGEISALAFSGI
                     LSDEEAFRLVCKRGELMDKAATENPGAMAAVLKLTPEKVEEICSRFDKTYPVNYNSPA
                     QTVVATTSENADAFCEAVKAEGGRAKLLAVSGAFHSPFMADAAEGLGKYMENVDFAQP
                     KVQIYSDYTAQPYSGDFKTLVKAQVENPVKWQTIVENMINDGVDTFIEVGVGKTLTGL
                     VKRINGDVKAFKVETAADIDALEL"
     gene            7760..8503
                     /gene="fabG"
                     /locus_tag="PLS64_01880"
     CDS             7760..8503
                     /gene="fabG"
                     /locus_tag="PLS64_01880"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009254853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRL42615.1"
                     /translation="MRFENKTAVVTGGSRGIGLAIATKLAKEGANIAILYVGDENEGI
                     KAKEELSQYGTKVEQYFCDVSNFEESKKVVDKVIEEFGSIEILVNNAGITRDKLVLNM
                     DEKDFDAVINVNLKGTFNMIKHTYKHFMKKRYGRICSTASIVGLTGNAGQANYSASKA
                     GIIGLTKSVAKELAGRGVTANAVAPGYIATDMTSVLPDKVKDAMKAQIPAKRIGTPDD
                     VADVVAFLCSDDAAYVTGEVIRVDGGLAM"
     gene            8521..9750
                     /gene="fabF"
                     /locus_tag="PLS64_01885"
     CDS             8521..9750
                     /gene="fabF"
                     /locus_tag="PLS64_01885"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRL42616.1"
                     /translation="MSRRVVVTGMGAISPIGNDVPTMWKNMVDGVCGIETITAFDTSD
                     LKVHIAGTVKDFDPTQYIEKKEVRKLDPYTQYAIAAAQQAVDDSGIIGKIDENRFGVY
                     IGAGIGGLQTFVKNTVGMENGGPRKVSPFFIPMMIGNIATGNVAIRFNAKGVSLSVMS
                     ACATGTNSIGEAFHCIKDGYADAIIAGGAEAVVAPLTIAGFQNMKALSTNDDPKHASR
                     PFDKNRDGFIMGEGAGMLVLEEYEHAVARGAKIYAELSGYGNTCDAHHVTAPDPNGAG
                     LARAIKIAFEEANVADDAQLYINAHGTSTHLNDLTETMAIKTALGEKAYDANISSTKS
                     MTGHLLGATGAVEAIASVMALNDGVIPPTINLDEPDEGLDLNYTPNKAVKRDVNVAAS
                     TNLGFGGHDACLVFKKY"
     gene            9765..10238
                     /gene="accB"
                     /locus_tag="PLS64_01890"
     CDS             9765..10238
                     /gene="accB"
                     /locus_tag="PLS64_01890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HRL42617.1"
                     /translation="MNKIEEIKEYISLLENSSLSVLEIEKEDGTKIHLEKEHPAQQIF
                     NASPIGAYQPTATDVPAPAPVQPQAPAAQSAAPEEDAKTVNAPIVGVFYAASAPGKAP
                     FAPVGKKVSKGSTVCIIEAMKCMNEIQAEDDYEIVEVLVKDGDLVEYGQPLFKVK"
     gene            10251..10670
                     /locus_tag="PLS64_01895"
     CDS             10251..10670
                     /locus_tag="PLS64_01895"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HRL42618.1"
                     /translation="MNQEEIKKILPHRDNMLLVEEAESVDENTAKGRYTIKGDEFFLQ
                     GHFPGNPVVPGVILCEMMGQASCCLLADKVKNCTPYFTKMNNVKFRNPVKPGDTLESV
                     CTLTKSRGAFYFIDAKGYVDGKLCVSAELSFALVENK"
     gene            10740..12098
                     /gene="accC"
                     /locus_tag="PLS64_01900"
     CDS             10740..12098
                     /gene="accC"
                     /locus_tag="PLS64_01900"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HRL42619.1"
                     /translation="MFSKILIANRGEIAVRIIRACREMGISTVAIYSEADKDSLHVQL
                     ADESYCVGGNRVADSYLNMPAILTVAVESGAQAIHPGYGLLSENAKFVSLCEQCNINF
                     IGPSSEMIKLLGDKDNARKTMRAAGVPVTPGCDIVESVEQAKEEAEKIGFPLLIKARS
                     GGGGKGIRRVDSIEQFEEAFLSASSEAQSAFGDGACYIEKFIHPVKHIEMQLLCDKYG
                     NIVCLGERECSVQRKNQKMIEESPSPALDEKTRKEMMIAAVKAAKAVNYVNAGTVEFL
                     LDMDNNFYFMEMNTRLQVEHGVTEMVTGLDIVQWQIRIAAGAKLSRTQDSIFTHGNVI
                     ECRINAENPEKGFRPSCGRITRLHTPGGAFVRFDTAIYQDYFVPPYYDSMIGKLIVQA
                     RSRAEAIRKMKMALSELTIDGIDINRDLLAEILSDEQFVSGEYTTDFMAEFEEKHADR
                     KK"
     gene            12104..12967
                     /gene="accD"
                     /locus_tag="PLS64_01905"
     CDS             12104..12967
                     /gene="accD"
                     /locus_tag="PLS64_01905"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HRL42620.1"
                     /translation="MDLFAFRKPKNELESDEGESKNVPFVPEEMWIKCPKCNTMLLTT
                     DMEENLHVCTKCNHHFRMNGRDRVEMLADKDTFVEFDADLSSHNILDFPGYDEKLEKA
                     RKTGAKESVICGECKMNGIDTVLCVMDPTFMMGSMGVITGEKITRAFEYATENAMPIV
                     VCTAAGGARMQEGILSLMQMAKTSGAVKRHSDAGNLYITVLTDPTTGGVTASFAMEGD
                     IILAEPDCLVAFAGPRVIEQTIRQKLPKDFQTSEFVLQKGFIDSVVSRNDLKSTLVKL
                     LKLHGYSGEEA"
     gene            12970..13752
                     /locus_tag="PLS64_01910"
     CDS             12970..13752
                     /locus_tag="PLS64_01910"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HRL42621.1"
                     /translation="MTAYEKVMAARDANKLTSVDYIAHMFGDSFIELHGDRRYADDKA
                     VVAGLAMLFDTPVTVIGIEKGRNTKERVERNFGQAHPEGYRKALRLMKQAEKFRRPVL
                     CLVDTSGAYPGIGAEERGQGQAIAENLMEMMTLKTPVLTVLIGEGGSGGALALAVSDE
                     VWMLENSVYSVISPEGCASILWKDSSKAADAAEALRLTAQDLFDLGVIERIIRQPKAG
                     DHAMFESLKMLIKRTFEKNLALDDEELLNARYERFRKIGRPE"
     gene            complement(13839..14276)
                     /locus_tag="PLS64_01915"
     CDS             complement(13839..14276)
                     /locus_tag="PLS64_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42622.1"
                     /translation="MKCPNCGFETESAFCQMCGTKISEALHTQNNETTETEYTSPLPQ
                     TGFEQAEGFAPQIQNEMPELEINQPPIPYIPPAEQPIDKKKAFKIVKIVAACVIGAVI
                     IAGIAINVTAAISGNSKSIFGNNSISDWVNRPEMHDGDYSTYN"
     gene            14629..16170
                     /locus_tag="PLS64_01920"
     CDS             14629..16170
                     /locus_tag="PLS64_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRL42623.1"
                     /translation="MRGLYSSKTKIRNQIFTEVARFAYEGGDYSKFENLPYEIIPGEI
                     STYRESIFLERAIVGERLRLAMGLPLLPVSKQAPISTGVEESMIDEKVYDPPLINIIK
                     FACHKCAEKRVVVTDGCQGCLEHPCTEVCPKGAISIVHGKSHIDDEKCIKCGKCQGAC
                     PYNAIIKQERPCAAACGMKAIHSDEHGRADIDYNKCVSCGMCLVSCPFSAIVDKSQIF
                     QTIMAINSGTPVYAAVAPAIAGQFKGLPSNKIRNAFKALGFTDVVEVAVGADLCTVEE
                     AKDFMEEVPEKQPFMATSCCPAWSVMAKKTFPDIAPYISMALTPMVLTGRLTKQHYPD
                     CRVVFIGPCAAKKLEASRRSIRSDIDFVLTFEEVAGMFAAKEVDFNAVEVDEKPLSFS
                     SADGRGFAVSGGVAKAVVNAIHKLDPEREVKVANAQGLDECVKLLRMAKAGKYNGYLL
                     EGMACPGGCVAGAGTINNPDKSAMEVLKSKKESCFEGAVDTPFIDRLSTLENMYNEFD
                     KMKEI"
     gene            16193..16963
                     /locus_tag="PLS64_01925"
     CDS             16193..16963
                     /locus_tag="PLS64_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRL42624.1"
                     /translation="MVMLILRIIGIAAAVVFLGFFVIPMTHGIINPGNIAGTFLCLWL
                     LCVLIPPVHGAIKAFCFKHLFTKIVFRVVNIGFIAFAVYGAIVTSAMVWATCQAPPEN
                     STAIVLGAQVKPWGPSVILRGRIDAAKSYLDDSPNANAVLSGGQGSDEIMSEAQCMYN
                     VLTESKIDSSRLYMEDKSTNTTENIRNSMKIIEDNNLNTDITIVTDGFHQLRARIICS
                     QLGIKGNVGAVSSDTRLLYVPTYAVREWFALPFQLLFR"
     gene            complement(16908..17159)
                     /locus_tag="PLS64_01930"
     CDS             complement(16908..17159)
                     /locus_tag="PLS64_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42625.1"
                     /translation="MSFYDVNHNEGQHEKYPIELPSRNSKKEISPIVDVTEHEVYPLE
                     FANCHRTKPHFDTNGDITEIVTTETEAETEERTTHEQHK"
     gene            17408..>19010
                     /locus_tag="PLS64_01935"
     CDS             17408..>19010
                     /locus_tag="PLS64_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42626.1"
                     /translation="MTKKIMSMILSIAMVLSIAVVGISGVSAAVDSDGRYVPSEGVET
                     HRVYFAMPNSWKSAKYGADSAGIFWWNGSDTDGFVEGTERLAWPGYKMQVADAESNLY
                     YVDLPTDVIAVIFNNALNGGERIVDDYGNVTYEFSEGQFMAANQTADIISGSYSEGDD
                     DYYDNMLDGEFWSKAKAALNGDDKSFLGNFADNFYEDPDWGIAMNFDNMVYIVDLNNA
                     AENLVSGKKFYYGEYYFFYGKNEKTGKYEYGGLPNYEMAKENGVLYELNDTSTPDEPS
                     SEPTTTPEPTTPEPAVKEIKFDVKKSGWENVKNIYCHVWAADGTGVWPMWQTRSEKCV
                     YDPDTGIAAYDLSKTGNTISASDGKRYCVIFSANTGVQTYNVVMDGKCIGDTLYCTGN
                     KLENPDDSEKVALEAVWTENKDCGPEKRITTTGNVIGTTLTEGVTDETLLAQYLINYY
                     DFPEKSAYTQNLLNALLVSPKDVKTAAESILDSSDYLDKDTILSKVEEILAECSDPLE
                     SMIYGDVNNDGIITIVDATIVQKYIV"
BASE COUNT         5374 a         3947 c         4411 g         5278 t
ORIGIN      
        1 tgtcaagcct cgttgttctc cgctattcct aaaataactt ccactcgtct aatccgtttc
       61 aattactggc gtaattttca cagcttagtc ctcgccggtc gttattttag ggctgcgaac
      121 ctcggctctt ctattagtca aagaatttgc tgtggatagc gcttacagcc ttgtcagcgt
      181 ctgctgaatc aataagaacc gaaaccttga tttcgcttgt gctgatcatc tggatgttga
      241 cctgagcgtc ataaagagcc tcgaacatct ttgtagcaac gcctgcgtgg ctttccatac
      301 ctgcgccgac gattgaaatc ttagctacct tatcgtcata gagaatttcc tctgcaccga
      361 ggttctctgt gtattccttc ataagagcaa cagcctcttc gcctctgttc ttggcaactg
      421 taaagctgat gtccttcttg ccgtcatgac cgattgactg aagaataatg tcaacattga
      481 tatctttagc agaaagcttt gagaacatct taaaagcaag accggggata ttcggaacac
      541 ccataacaga aattcttgca acatctgtat ctttagcaac accgctgatt aacatttttt
      601 ccattttagt tttctccttt acaattgtac ccgaagcctt tgtcatactt gaaagcactt
      661 caagctcgat attatatttt ttagccattt caaccgaacg gttgttaaga acctgagcac
      721 cgagtgtagc aagctcaagc atttcgtcat aagagatttc ctgaagcttc ttagcgttct
      781 taacctttct cggatcagct gtgtaaacac cctcaacatc ggtgaaaatc tgacagagat
      841 cagcgtgcat tgcagccgca atagctacgg cacttgtgtc tgaaccgcct ctgccgagtg
      901 ttgtaatatc gccgtatttt gaaatgccct ggaagcctgc aacgattaca atgttcttct
      961 tgtcaagctc tttcttaatt ctgtcaggct caacttttct gattcttgcg cttgtgtgag
     1021 cagaagtggt attgaaacct gcctgccagc caagaagaga aacagcgtgg taaccgagct
     1081 tttcgattgc cattgcaaga agtgaaatag aaatctgctc gcctgcggca agaagcatat
     1141 ccttttctct ttttgaagcc tttggattga tttcgtttgc cttttcaatg agatcgtctg
     1201 ttgtgtcgcc ctgagcggat acaacaacta caacatcatt gccctttgcg taagtgtctg
     1261 ttacgattga tgcaacattc attactcttt cggcattggc aacagagctg ccgccaaatt
     1321 tctgaactat caaactcatg tacttaatac tccctctgta aaattataaa tcagccacac
     1381 ggatacttga aagtaccgaa acaccgtcag cctcaagctg agcaatcttc tcggcaaatt
     1441 cgttgtacgg catagccttg acaacaaatg ctttttcgtc ggcaggtgcg tcagccttat
     1501 tgattactgt tacctcaccg aataattttt ctgccttttc tgcaacatta ctgccgtttg
     1561 cacgaacata catggcacgg cttgactcct cccaaggaag aatgtttttg ccgttgccgt
     1621 ctgcccagta aaggaacttt cttgtcttga ggtgcttaca gcagtcgatt acatcggcta
     1681 caactgcgct tgctgtagga agcttgcctg cacccttgcc gtagaatact acatcgcctg
     1741 tgcagtcgcc tcttaccata atgccgttga actcatagtc aatgttagca agctggctct
     1801 tgttcggaac gagcatagga gcaacgataa tgtcaatctt gccgtccgca agacgcttaa
     1861 cactgccgat aagtttaatt acgcaatgga aagcctcagc gtatcttaca tcttcaagtg
     1921 taatttttgt aatgccctct gtatgtacag agtcgggata aacatgcttt ccgtatgcaa
     1981 gagatgcaag aatacaaatc ttacggcaag catcgtgacc ttcgatatca gcggcaggat
     2041 ttctctcggc aaagccaagc tcctgagcaa gcttgagcgc atcgtcaaac tgcataccgt
     2101 cttcaatcat ctttgtaagg ataaagtttg ttgtaccgtt aagaataccg gcaatctcgt
     2161 caacattgtt tgcaacaaga cactggctca tcgggcggat aatcggaata ccgccgccta
     2221 ccgatgcttc aaagaggaag tttgtgtttg tttcctttga aatttcaagg agttctgcac
     2281 cgtgagcggc aacaagctcc ttgtttgaag taacaacact cttacctgct ttaagacatc
     2341 tctttacaaa gtcataagca ggattaagac cgcccataac ttcaacaaca actcttattt
     2401 cgaggtcgtt gatgatatct tcaaagtttt ttgtgaatct gtctgcaagc gggctgtcgg
     2461 gaaattcacg aaggtcaaga atcttcttga cataaacctc ttcaccgatg cttgcataaa
     2521 gcttctgttt gtgtgttgta aagatttcgg caacgcccga acctacaaca ccgtgaccca
     2581 taattgctat tgaaaccata tctaaaaacc tttcctttcg agtttttggt tttaacctgt
     2641 tattttattc tgcggcaggg ggagcttcct caccgccgcc tgacgattca ccgcctccgg
     2701 agctttcgcc tccgcctgag gattcccctc cgccggaggt gtcgccgcct gaattatctc
     2761 ctccgctgtt gcttgtgtcg ccgccgtaat tgctaccgct gttgtcaccg ccgtaattgg
     2821 gattgtagta gcttgaggag tctgaaacat ctgagccgta ttcatagtta ccgctgtaat
     2881 atgaacctcc cgaactcggc atattgtcct cggtgtagta gcccacatac cgtcccgaaa
     2941 tattatctgt tgaaacaata agtcccgttg taggattata tgttgcctca atcaggttat
     3001 cggatatttt atattccttg ttttctttgc ctttaaggta cttgcccata acatttgcaa
     3061 agaattttgt tgccgttgtt cttccgctgt aggaaatggt tttaggctta tcaaaaccgc
     3121 accatgtagc cattacggca tatggtgaac atccgcagaa ccatgaatcc ttatcgtcat
     3181 cggttgtacc tgttttgccg ataatgtccc agcctgaaat ctgagcgtaa tgtgcgtttg
     3241 tgtgagcgct gtttgtaaca ttgtagtgaa gcagtctgtt catgattcct gctgtttcgg
     3301 ctgaataagc ctgtttcgga actgcgtcac ggttatcaat gattatgttg tcctctgagt
     3361 cggttacata gtagtatgtg tacggttcgt agtaaagacc gccgttgccg agatatgtgt
     3421 aagcagccgc catttctctt actgtaacac cgccgttcat accgccgatt gaaagagcac
     3481 ccgtattctg tgcatcctgt tccgtaagat tctggaatcc catttttgta acaacctgct
     3541 gatatgcaac gctcggaccg ccgataagct ccatggtctg agcggctgtt gcgtttgatg
     3601 accactcgat tgcatcggga agactcatat tgccgttgta gtagccgtat gcgttgttag
     3661 gacccgaaac aagctgaccg ctttcatttg tacgatagtt gtcaagcggt tcatcaagaa
     3721 ccattgatga gaaataaagc ttctttgatt cgattgcata agggtaaaca acaacaggct
     3781 tgattgaaga acccggctga agtacggagt tgtaggatct gtcccagctg agagcgccct
     3841 ctttctttgt tgaagagccg actgttgcaa ttactctgcc gtcatatccc ataattgttg
     3901 acgcaatctg aaggtcggaa tcatttgatt tatcaatatt gagtgccgca ttttcaaggt
     3961 aggtctgagc attttcatcc attgcacagt agattttaag tccctctgtg taaagctttg
     4021 aagatgccgc agattcgctt atgttgtaat attctgcaat gtccttttga agatcacgaa
     4081 agacctgatc aatataccag ttctgaacat cagcctcgtc atcatcgtca tcgtcatcgg
     4141 acgcctgcca gccgacaaat ttcattgtgg cactttcttt catagccgca tcaaattcgt
     4201 ctttagaaat ttttttctga tcatacattt cattgagaac gatttcacgg cgctccttgt
     4261 tgttttcggg gaaaacaagc ggattccatc ttgacgggtt ttgtgtaatt cccgcaattg
     4321 ccgcacattc tgcaattgaa cagtccttta tgcttttgcc aaagtaaagc tgagccgcag
     4381 attcaacacc ctgacagtta ttgccgaagt ttacaacatt gagataggct tcaagaatct
     4441 gatcctttgt atattcctgc tcaagcttta gtgcctttgt gatttcacga agttttcttg
     4501 taattgaaac ctcgttgtca tctgtaatgt tcttaatgag ctgttgggtg attgttgaac
     4561 cgccgtatga gtccgaaccg gttgcaagat ttgcaacggc ggaaagtgtt ctgccccagt
     4621 caacaccgtt gtggtcaaag aaacgcttgt cctcaattgc gacaaccgca tctttcattg
     4681 cctgaggaat atcctgattg tcaacccaga ttctgttctc ggtatcgtat agtttctgat
     4741 actccttaaa ttcctttgta tcctcattct gaatatatat tcggcttgtc tggttcatag
     4801 actttgcctt gaggtcgatg cccgtaggct cggacgcaat ggtgaaaatg tagacagcaa
     4861 gtgaaatgcc cgtaataagc agagcaacca ggcataccga acaaacggta atcaggaact
     4921 tgccaaaacc cctgaaaaat ctttttacac ctccgtcagc tttttcggat aattcatttt
     4981 gctgaatatc cttcttatac tgactgatgt cagtttcacg cttgtttcgc atattatctc
     5041 ctttatcaat tcacaattta cggttaaacg aacaaccgtc ggtttgtaat ttgtctgagt
     5101 gatttgcaca aacgcagtgc gccggctgta atacataaat tatataataa catatatgtt
     5161 caaaattaca agcagaaata ctcatttgat tattatatca cctgaaaaga aaaaaataaa
     5221 tatattaatg caaatttctc ttaaaaaata tgcagaatta aggcggtctt ttggttggaa
     5281 ttttataaaa atgatgaata taaccgttgg ttcgggaaaa aataaaagta aaaactgacg
     5341 gaaagagtgc tgtattatga aaaaaatagt tggattaaca ggtgtgattc tgattgcgtg
     5401 tatgatgata agctcgcttt ttgctcccgt gaattctgcg gaaaacacct ctgcaacagg
     5461 caattattcg tcgtcgcaga ataacggtac aacggaaggc gactacatca taaaagccga
     5521 aaaagggtac attgtagtgt acaaaaaagg cgaggacaaa gcgtatctga caaccgactg
     5581 cagagtatca aatcttccga aaggcgacgc actttacctt gaaaaaggaa ttgaaataaa
     5641 gggaaaagaa aaccttatga gggctcttga ggactattgc agttaaaggc ttaatggtaa
     5701 gataattctt catatatatt gacttttctg tgcttttata ttgacttttc tgtgcttttg
     5761 gagtaaaatc agactgatat gccataaagt atggtatcag gtgcagaatt tgccgtaaat
     5821 ttttcaaaat aatgaaatct tgcaaatttt ctgtttctga aaatcaagta aaaatgcaga
     5881 aaaggagata ttacatatga tttctactgt cattgaccag tcgctcggtc ttgagttccc
     5941 tatttttcag ggaggcatgg cttgggtggc tgatgcatcg cttgccgcag gtgtttcaaa
     6001 cgcaggcggt ctcggcatta ttgcagctat gaattccaac ggcgaacagc tccgtgagga
     6061 aatcagaaag tgcaaaacaa tgaccgataa aattttcggc gtaaacatca tgcttatgag
     6121 tccgtttgca gaggaagtaa gcgatgttgt agttgaagag ggcatcaagg tcatcacaac
     6181 aggtgcaggc aaccccggca agtacatggc aaagtggctt gaggcaggca tcaaggttat
     6241 tcctgttgtt ccgagcgttg cgcttgcaag gaaaatggaa aaagacggtg cttttgccgt
     6301 tatcgcagag ggcggcgagt caggcggtca tgtcggtgat ctcacaacaa tggctcttgt
     6361 tcctcaggtt gttgatgcag taaatatacc tgtaattgcc gcaggcggta ttgcagacgg
     6421 cagacagatt gccgcagcat ttatgctcgg cgctcagggc gttcaggtcg gcacaagatt
     6481 tctcgttgca aaggaatgta ctatctctca ggaatataag aacaaaattc tcaaagcaag
     6541 agatattgac acggttgtaa caggtaaaag actcggtcat cctgttcgtt caatccgtaa
     6601 ctcgtttacc cgtgaatata caaaggctga gtacgattcg gctaatgttt ccgatgagga
     6661 gcttgaaaat atgggactcg gcagactcag aagagccgtg agagagggcg atgtttctca
     6721 gggtaccgtt cttgcaggac aggttgcaag tatggttaaa aaggaacagc cggcaagaga
     6781 aatgattgaa gagatgttct cacaggcaga agaagtattg aacggagcaa caaaatgggt
     6841 aaaatagcat ttattttttc aggtcagggc gcacagtaca gcggtatggg caaggatctt
     6901 tacgataatt cgcctgctgc aaaggctgtt tacgaaatgg ctgattctat aagagaaaac
     6961 acttcaaagc agtgctttga gggtacaatg gaggagcttt caaagacagt aaatacacag
     7021 ccgtgtattt ttactgccga ccttgcggcc gcaagagctc ttgttgaaaa gggtatcact
     7081 cccgattgcg tagcaggctt ttcactcggt gagatttctg cacttgcttt ttcgggaatc
     7141 ctttccgatg aagaggcttt cagactcgtt tgcaaaagag gcgagcttat ggacaaagcc
     7201 gcaacggaaa atcccggtgc aatggctgcc gtgttaaagc ttactcccga aaaggttgaa
     7261 gaaatctgct caaggtttga caaaacatat cccgtaaact acaactcacc ggctcagact
     7321 gttgttgcaa caacaagtga aaatgccgac gctttctgcg aggcagttaa agcagagggc
     7381 ggaagggcaa aacttcttgc agtaagcggt gcgttccatt ctccctttat ggctgatgcc
     7441 gcagagggac taggaaagta tatggaaaat gttgactttg ctcagccaaa ggttcagatt
     7501 tattccgatt acacggctca gccgtattcg ggcgatttca aaacgcttgt caaagcacag
     7561 gttgagaatc ccgtaaaatg gcagactatt gttgaaaata tgattaatga cggcgttgac
     7621 acctttattg aggttggtgt gggcaaaact ctcacaggcc ttgtaaagag aatcaacgga
     7681 gatgtaaagg cattcaaggt tgaaactgcg gctgatattg acgcacttga actttgatac
     7741 tgttcggagg tataatataa tgagatttga aaataaaaca gccgtagtaa caggcggctc
     7801 aagaggtatc ggccttgcaa tcgcaacaaa gcttgcaaaa gagggtgcta acattgcaat
     7861 tctctatgta ggtgacgaaa acgagggcat taaggctaag gaagagcttt cacagtacgg
     7921 cacaaaggtt gagcagtatt tctgtgatgt ttcaaacttt gaagagtcaa agaaggttgt
     7981 cgataaggtt atcgaggagt tcggcagtat cgaaattctt gtaaacaacg caggcatcac
     8041 aagagataag ctcgttttaa atatggacga aaaagatttt gacgctgtaa tcaatgtaaa
     8101 cctcaagggt acattcaaca tgattaagca tacatacaag cactttatga aaaagcgtta
     8161 cggcagaatc tgcagcacag cgtcaattgt cggtcttacc ggcaacgcag gtcaggctaa
     8221 ctattctgca tcaaaggccg gtattatcgg tcttacaaag tcggttgcaa aggaacttgc
     8281 aggcagaggc gttacggcta atgcagttgc tccgggttac attgcaacag atatgacaag
     8341 tgtacttccc gataaggtaa aggacgcaat gaaagcacag attcctgcaa agcgtatcgg
     8401 tactcccgat gatgttgcag atgtagtagc tttcctctgc tctgacgatg ccgcttatgt
     8461 aacaggcgag gttatcagag ttgacggcgg acttgcaatg taattgattt ggaggatata
     8521 atgagcagaa gagtagttgt aacaggtatg ggcgcaattt caccgattgg taacgatgtg
     8581 cctacaatgt ggaaaaatat ggttgacggc gtttgcggta tcgaaacaat cacagctttc
     8641 gatacaagtg acctcaaggt tcacatcgca ggtacagtca aggattttga tcccacacag
     8701 tatattgaga aaaaagaagt aagaaagctt gatccatata cacagtacgc aatcgcagcg
     8761 gcacagcagg cagttgacga cagcggtatc ataggcaaga ttgacgagaa ccgtttcggt
     8821 gtttatatcg gcgcaggtat cggcggtttg cagacctttg ttaagaatac agtgggcatg
     8881 gaaaacggag gcccgagaaa ggtttctcct ttcttcattc cgatgatgat cggtaatatc
     8941 gcaacaggca atgttgcaat ccgttttaac gcaaaaggtg tgtctctttc ggttatgagc
     9001 gcctgtgcaa caggtacaaa ctcaatcggt gaggctttcc actgcatcaa ggacggctac
     9061 gctgacgcta tcatcgcagg cggtgcagag gcagttgttg ctcctctcac aattgcaggt
     9121 ttccagaata tgaaagctct ttcaacaaac gatgatccaa agcacgcatc ccgtccgttc
     9181 gacaagaatc gtgacggttt catcatgggc gagggcgcag gcatgctcgt tcttgaagag
     9241 tatgagcacg cagttgcaag aggtgctaag atttatgcag agctttcagg ctacggcaat
     9301 acttgtgatg cacatcatgt tacagctccc gatcctaacg gtgcaggtct tgcaagagca
     9361 attaagattg cttttgagga ggcaaatgtt gccgatgacg cacagcttta catcaatgct
     9421 cacggcacaa gcacacatct taacgacctt accgaaacaa tggctatcaa aactgctctc
     9481 ggtgaaaagg cttatgatgc aaatatcagc tcaacaaaat caatgacagg tcatcttctc
     9541 ggtgcaacag gcgcagttga ggctatcgct tctgttatgg ctctcaatga cggtgttatt
     9601 ccgccgacaa tcaaccttga cgaacccgat gaaggtcttg accttaacta tacacctaac
     9661 aaagcagtta agcgtgatgt aaatgttgcc gcttcaacca acctcggctt tggcggacat
     9721 gacgcttgtc ttgtatttaa aaaatattaa gcgagggaat tactatgaat aaaattgaag
     9781 aaatcaagga atatatttcc cttcttgaaa attcttcttt gagcgttctt gaaattgaaa
     9841 aagaggacgg tacaaagatt catcttgaaa aagaacatcc ggcacagcag atttttaatg
     9901 cgtctccaat cggtgcatat cagccgactg caacagatgt tcccgctcct gcgcctgttc
     9961 agccacaggc tcctgcggct cagtctgccg ctcccgagga agacgccaag actgtaaacg
    10021 ctccgattgt cggcgtattc tatgccgcat cggcaccggg caaggctcct tttgctcctg
    10081 tcggaaagaa agtaagcaag ggctcaaccg tttgcattat cgaggctatg aagtgcatga
    10141 acgaaattca ggctgaggac gactatgaaa tcgttgaagt tcttgttaag gacggagatc
    10201 ttgttgaata cggtcagccg ttattcaagg ttaaataatc ggaggactgt atgaatcagg
    10261 aagaaattaa aaagattttg ccgcatcgtg acaatatgct tcttgttgaa gaggctgaaa
    10321 gtgttgacga aaacacggca aagggcagat atacaatcaa aggtgacgag ttctttttac
    10381 agggacattt ccccggtaac cccgttgttc cgggtgttat tctctgcgaa atgatgggac
    10441 aggcaagctg ttgccttctt gcagacaagg ttaagaactg cacaccttac tttacaaaga
    10501 tgaacaatgt taagttcaga aatcccgtga aaccgggcga cactcttgaa agtgtctgca
    10561 cactcacaaa gagcagaggt gcattctact ttattgacgc aaagggttat gttgacggca
    10621 agctctgtgt aagtgccgag ctttcgtttg cacttgttga aaataagtga ttttaaaggc
    10681 tgtctgtgcc gttttgggtg cagacagccg taatgcgata aataactgga agtgaaaaaa
    10741 tgttctcaaa aattctgatt gccaaccgag gcgaaattgc agtaagaatt atccgtgcct
    10801 gccgtgaaat gggcatcagc acagtagcaa tttattctga ggcagacaag gactccctgc
    10861 atgttcagct tgccgatgaa agctactgcg taggcggtaa ccgtgttgcc gacagctacc
    10921 tcaatatgcc tgcaattctc accgttgccg ttgaaagcgg tgctcaggct attcatcccg
    10981 gctacggact cttgtccgaa aatgccaagt ttgtttcgct ctgtgaacag tgcaacatca
    11041 actttatcgg tccttcatcc gaaatgataa agcttttggg cgacaaggac aacgcaagaa
    11101 agactatgcg tgccgcaggt gttcccgtaa cccccggctg cgatattgtt gaaagcgttg
    11161 aacaggcaaa agaggaagct gaaaaaatcg gttttccgct tctcatcaag gctcgttcgg
    11221 gcggcggcgg aaagggtatt cgccgtgttg attcaataga gcagtttgaa gaggcatttt
    11281 tgtcagcttc aagcgaggcg cagtcagcat tcggtgacgg tgcctgctac atagaaaaat
    11341 tcattcaccc tgtaaaacac attgaaatgc agctcttgtg tgataaatac ggcaatattg
    11401 tttgtctggg tgagcgtgaa tgctctgttc agagaaagaa ccagaagatg attgaagaaa
    11461 gtccaagccc cgctcttgac gagaaaaccc gtaaagagat gatgattgcg gctgttaagg
    11521 cggcaaaggc agtcaactat gtcaatgcag gcacagttga atttttgctt gatatggaca
    11581 acaacttcta ctttatggaa atgaacaccc gtctgcaggt tgaacacggt gttacggaaa
    11641 tggtaacagg tcttgatatt gttcagtggc agataagaat tgccgcaggt gcaaagcttt
    11701 cccgcactca ggacagcatc ttcacacacg gcaatgtaat tgaatgccga atcaatgccg
    11761 aaaatccgga aaagggattc agaccgagct gcggaagaat tacaagactt cacaccccgg
    11821 gcggtgcatt tgtaagattt gatacggcga tttatcagga ctattttgtt cctccgtact
    11881 atgattcgat gatcggcaag ctgattgttc aggcaagaag ccgtgccgag gcgatcagaa
    11941 agatgaaaat ggctctttcc gagcttacca ttgacggaat tgacattaac cgtgaccttc
    12001 ttgccgagat tctctcggac gaacagtttg tgagcggtga atatacaaca gattttatgg
    12061 cagagtttga agagaaacac gccgacagga agaagtaaaa gatatggatt tgtttgcttt
    12121 cagaaaaccg aagaatgagc ttgagtccga tgagggcgag agcaaaaatg tacccttcgt
    12181 tcccgaagaa atgtggataa aatgccccaa atgcaacact atgctcctta cgactgatat
    12241 ggaggaaaat cttcatgtct gcacaaagtg caaccaccat ttcagaatga acggacgaga
    12301 cagagttgaa atgcttgccg acaaggatac ctttgttgag tttgacgcag acctttcaag
    12361 ccataatatt cttgattttc cgggatatga cgaaaagctt gaaaaggcga gaaaaacagg
    12421 tgctaaggag tctgttatct gcggagaatg taagatgaac ggcattgata ccgttctctg
    12481 cgttatggat ccgaccttta tgatgggaag tatgggcgta atcacgggcg aaaaaatcac
    12541 ccgtgcgttt gaatatgcaa ccgaaaatgc aatgccgatt gttgtttgta ctgccgcagg
    12601 cggtgcaaga atgcaggagg gcatcctctc gcttatgcaa atggcaaaaa cttctggtgc
    12661 ggtaaaaaga cacagcgacg caggtaatct ctacatcacc gtgcttacag acccgacaac
    12721 aggcggtgtt acggcatcgt ttgcaatgga gggcgacatt attcttgccg agcccgattg
    12781 tcttgttgcc tttgcaggtc cgagagtaat cgagcagaca atccgtcaaa agctccccaa
    12841 ggacttccaa acctcggaat ttgttcttca gaagggcttt atcgattcgg ttgtcagcag
    12901 aaatgatttg aaatctacac ttgtaaaact gcttaaattg cacggttaca gcggtgagga
    12961 ggcttaaaaa tgacagctta cgaaaaggtt atggcggcga gagatgctaa caagctgacc
    13021 tcggttgact atattgccca tatgttcggt gacagcttca ttgagcttca cggtgaccgc
    13081 agatatgccg acgataaagc agttgttgca ggacttgcaa tgctctttga cactcctgtt
    13141 actgtaatcg gaattgaaaa gggcagaaat acaaaagagc gtgttgaacg caacttcggt
    13201 caggctcatc ccgagggcta ccgcaaggct ttaagactta tgaaacaggc tgaaaagttc
    13261 agaagacctg ttctttgcct tgttgataca agcggtgcat accccggaat cggtgcggag
    13321 gaaagaggtc agggacaggc tattgccgaa aacctcatgg aaatgatgac tctcaaaact
    13381 cccgtactta ccgttcttat cggtgagggc ggcagcggcg gtgcgcttgc acttgcagtt
    13441 tccgatgaag tatggatgct tgaaaattcc gtttattcgg taatttctcc cgagggttgt
    13501 gcgtcaattc tctggaaaga cagctcaaag gcggctgatg cggcagaggc tctcaggctt
    13561 accgcacagg atttgtttga cctcggtgta atcgagagaa tcatccgtca gccaaaggca
    13621 ggcgatcatg ctatgtttga aagccttaaa atgctcatca aaagaacatt tgaaaagaat
    13681 cttgcccttg atgacgaaga gcttttgaat gcacgctatg agcgtttccg caaaatcggc
    13741 agacccgaat aatttatttt aaacctaaac ataaaaacgg caggaaagat aatttgcaat
    13801 actgcaatgc ctttcctgcc gttttatttt ttaatttttt aattatatgt gctgtaatct
    13861 ccgtcgtgca tttcaggacg attcacccag tcactaatgg agttgttacc gaaaattgat
    13921 ttgctgttgc ccgatatcgc cgcagttaca ttgattgcaa tgcctgcaat aatgaccgca
    13981 ccaatcacac aggcggcaac aattttcacg attttaaaag ctttcttttt atcaatcggc
    14041 tgttctgccg gcggaatgta gggaatgggt ggctgattta tttcaagttc gggcatttca
    14101 ttttgaatct gcggtgcaaa gccctccgcc tgttcaaagc ctgtctgtgg caaaggcgag
    14161 gtgtactcag tttcggttgt ttcattattc tgtgtatgta aagcttcgga aattttcgtt
    14221 ccgcacatct gacaaaacgc agactcggtt tcaaaaccgc aattaggaca tttcataggt
    14281 ttgtacctcg taatttttga tagtatgaca ccattttata acaatattca cttaaagtca
    14341 ataaatgtga caaaatcgct cggaataaag tttgatatta tgtaaataat atcgcaaaat
    14401 ttttgcaaaa taagcctgta tctgttgaaa acaagggtag tgtagtgtat aatctaagta
    14461 cgaattgcaa cattatgttg caattcagag attcaatgtt ccattgaacc tctgaggagc
    14521 taacgctcct atgtacaaga ttgcaagcaa ggcagagcgt tgctgcgatt taaatttagt
    14581 gaatgattga atagaaaata aaatacacac agtgaaagga tgagtgcctt gagaggactg
    14641 tattcgtcaa agacaaaaat ccgtaatcaa attttcaccg aggttgcaag gtttgcatac
    14701 gagggcggag attattcaaa atttgaaaat cttccgtatg aaattatccc gggtgaaatt
    14761 tcaacatacc gtgaaagtat atttctcgaa agagccattg tcggcgaaag acttcgcctt
    14821 gcaatgggac ttccgctttt gcccgtttca aaacaagcgc ctatctcaac aggtgttgaa
    14881 gaaagtatga ttgacgaaaa ggtttacgat ccgccgttaa tcaatataat aaaattcgcc
    14941 tgtcataagt gtgccgaaaa aagagtggtt gtgacagacg gctgtcaggg ctgtcttgaa
    15001 cacccgtgca cagaggtttg cccgaaggga gcaatctcga ttgtacacgg aaaatcgcat
    15061 attgacgatg aaaagtgcat caaatgcgga aaatgtcagg gtgcgtgccc gtacaatgca
    15121 atcataaagc aggagcgtcc gtgtgccgcc gcatgcggta tgaaagctat tcacagcgat
    15181 gaacacggca gagcagatat tgactataac aagtgcgttt catgcggaat gtgccttgta
    15241 agctgtccgt tcagcgcaat tgttgacaaa agtcagattt tccagactat tatggcaatc
    15301 aacagcggca cacctgttta tgccgccgtt gcacctgcaa ttgcaggtca gttcaaggga
    15361 cttccgtcaa ataaaatcag aaatgccttt aaggctctcg gctttaccga tgttgtcgag
    15421 gttgcggttg gtgccgacct ttgtacggtt gaagaagcaa aggattttat ggaagaagtt
    15481 cccgaaaaac agccgttcat ggcgacttcc tgctgcccgg catggagcgt tatggcgaag
    15541 aaaactttcc ccgatatcgc tccgtacatt tcaatggcac tcactcctat ggttctcacg
    15601 ggcagactta caaaacagca ttatcccgat tgcagagttg tgtttatcgg gccatgtgcc
    15661 gctaaaaagc ttgaggcaag ccgcagaagt atccgaagtg atatcgactt tgtgctgact
    15721 tttgaagaag ttgctggaat gtttgccgca aaagaggttg attttaacgc tgtcgaggtt
    15781 gacgaaaaac cgctttcatt ctcaagcgca gacggcagag gctttgccgt aagcggcggt
    15841 gttgcaaagg ctgttgtcaa tgcaatccac aagcttgatc cagaaagaga ggtcaaggtt
    15901 gccaatgcac agggacttga cgagtgcgta aagcttttgc gtatggcaaa ggccggcaag
    15961 tacaacggct atctgcttga gggtatggct tgccccggcg gatgtgttgc aggtgccgga
    16021 acaatcaaca atcccgacaa atctgcaatg gaggttctca aaagcaagaa agaatcgtgc
    16081 tttgagggcg ctgttgacac tccgtttatt gaccgtttgt caactcttga aaatatgtac
    16141 aatgagtttg ataagatgaa agaaatttaa caggtcgggt gatattttga aaatggtgat
    16201 gttaatttta cgcataatcg gcattgccgc agcagttgta tttctcggat ttttcgtaat
    16261 tccgatgact cacggaatta tcaacccggg caatatagca ggaacttttt tgtgtctgtg
    16321 gctgttgtgc gtgttaattc cgccagttca cggcgcaata aaggcatttt gctttaagca
    16381 tttgtttact aaaatagttt ttcgtgtagt gaatatcggc tttatcgctt ttgccgttta
    16441 cggtgcgatt gtcacctctg caatggtgtg ggcaacctgt caggctccgc ccgaaaactc
    16501 aactgcaatt gtcctcggtg ctcaggttaa gccgtgggga ccgtcggtaa ttttgcgtgg
    16561 cagaattgac gccgcaaaat catatcttga cgattctccg aacgcaaatg ccgttctcag
    16621 cggaggacag ggaagtgacg agattatgag tgaggcacaa tgtatgtaca atgtgctgac
    16681 ggagtcgaaa attgattctt caagacttta tatggaggac aagtcaacca acacaaccga
    16741 gaatataaga aattcaatga aaattattga ggacaacaac cttaataccg atattacgat
    16801 tgtgacggac ggttttcatc agctcagagc gaggataatc tgttcgcaac tcggcataaa
    16861 aggaaatgtc ggagcagtaa gctccgacac aagacttttg tatgtcccta cttatgctgt
    16921 tcgtgagtgg ttcgctcttc cgtttcagct tctgtttcgg tagtcacaat ctcggtgatg
    16981 tcaccgtttg tgtcaaaatg aggctttgtc ctgtgacagt ttgcaaactc aagcggataa
    17041 acctcgtgtt ccgtaacatc aacaataggg gaaatctcct ttttgctgtt ccgtgagggc
    17101 agctcaatcg gatatttttc gtgctgtccc tcgttatgat taacatcgta aaaactcata
    17161 ataaaaacca cctttccgtg aggtggtttt attttttgca gaattttaaa ttatatgcaa
    17221 agggaaaatt ttgttttatg gtaaaagttt acaaaaaaat gtatgaattg tcttaaattc
    17281 tattgacaaa atattgtatt agttgtatta tatgtatgag gttagaagtg cattgcagtg
    17341 caattttgca attcacaata catatactga atatttttta aaccgtaatc agaaagggga
    17401 ttttaaaatg acgaaaaaaa ttatgagtat gattctgtca atcgcaatgg tgctttctat
    17461 cgccgtagtc ggcatttcag gagtatctgc ggcagttgac agcgacggtc gttatgtccc
    17521 aagtgagggc gtagaaacac acagagttta ttttgcaatg cctaattctt ggaagagcgc
    17581 aaaatacggt gcagattctg cgggaatttt ttggtggaat ggatctgata ctgatggatt
    17641 tgtcgaggga acagagcgtc ttgcgtggcc gggttacaaa atgcaggttg ctgatgctga
    17701 atcgaatctt tattatgttg atctcccgac agatgtaatt gctgttattt tcaacaatgc
    17761 tcttaacggc ggtgaaagaa tagttgatga ttacggaaat gtaacatatg agtttagtga
    17821 gggtcagttt atggctgcaa atcaaaccgc cgacatcata agtggatctt attcagaggg
    17881 cgacgatgac tactatgaca atatgcttga cggcgaattt tggtcaaagg caaaagctgc
    17941 tttaaatggt gatgataaat cattcttagg taactttgcc gacaattttt atgaagatcc
    18001 cgattggggc atcgcaatga attttgacaa tatggtatat attgttgacc ttaacaatgc
    18061 tgctgaaaat cttgtttcag gtaagaaatt ttactatgga gaatattatt tcttctacgg
    18121 caagaacgag aaaacaggca agtatgaata cggcggactt cctaattatg aaatggcaaa
    18181 ggaaaacgga gtgctttatg agcttaatga tacatcaact cccgatgagc cttcgtcaga
    18241 acctacaaca actcctgaac cgacaacacc cgagcctgct gtaaaggaaa tcaaatttga
    18301 tgttaaaaaa tcaggttggg aaaatgtaaa aaatatttat tgccatgtat gggctgctga
    18361 cggtacaggt gtatggccta tgtggcagac aagatcagaa aaatgtgtct atgatcccga
    18421 tacaggaatt gccgcatatg acctttcaaa aacaggcaac acaatttctg cttcagacgg
    18481 aaagcgttat tgcgtaattt tcagtgcaaa cacgggtgtg cagacatata atgtagttat
    18541 ggacggcaag tgcattggtg atacactata ttgtacaggc aataagcttg aaaatcctga
    18601 tgattctgaa aaagtcgcac ttgaggcagt ttggactgag aacaaggatt gtggtccgga
    18661 aaagagaata actacaacag gcaatgtaat cggaactaca cttaccgagg gtgtgactga
    18721 tgaaacactc cttgcacagt accttattaa ttactatgat tttcctgaaa aaagtgccta
    18781 cacacagaat cttcttaatg cattgcttgt aagtccaaaa gatgtgaaga ctgccgcaga
    18841 aagtattctt gatagcagtg attatcttga taaggataca attttgtcca aggttgaaga
    18901 aattcttgct gaatgttctg atcctttgga aagtatgatt tacggcgatg tgaataatga
    18961 cggcataatt actattgttg acgcaacaat agtgcaaaaa tacatagtaa
//
LOCUS       DAOMVE010000017        17904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_603_length_17904_cov_3.943358, whole genome shotgun sequence.
ACCESSION   DAOMVE010000017 DAOMVE010000000
VERSION     DAOMVE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17904
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_603_length_17904_cov_3.943358"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..434)
                     /locus_tag="PLS64_01940"
     CDS             complement(156..434)
                     /locus_tag="PLS64_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HRL42627.1"
                     /translation="MYEDKTLVCKECGNEFVFTAGEQEFYAEKGFTNEPQRCKACRQA
                     RKNAAKTEREFFETVCAECGGTARIPFKPVEGKSYYCSECFAKRKAAE"
     gene            complement(540..1130)
                     /locus_tag="PLS64_01945"
     CDS             complement(540..1130)
                     /locus_tag="PLS64_01945"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL42628.1"
                     /translation="MTELQKSYGTLSDAQLANFVSLGDDRAFDELVVRYLDTISIIAR
                     KFSAEGYEHNDFVQEGLVGLLCSCKAFDQNGGASFKSYMSVVVERRFISIIRRGEQKK
                     AIPSSSIVPMDDVGESIEDSAKTPEELLMCREQLDQLLLRLQSVLSKSEYDVLMLYGN
                     GLSYKEIAEKLSVTEKAVDNALQRARKKVHGGLNVG"
     gene            1369..2898
                     /locus_tag="PLS64_01950"
     CDS             1369..2898
                     /locus_tag="PLS64_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRL42629.1"
                     /translation="MVVTCNSFGVSGMDGYKVELEASMYNGTREFDMVGLPDAAVRES
                     KDRVLSALKNCGFRYPAAHMTVNLAPADIKKEGPIYDLPIAVAILILLNQIKSNISDC
                     AFIGELSLSGEVRGINGVLPMVIKAKECGIKKIYVPKANASEGAVVDGIEVYGIDNIL
                     QLKDYLNHILEPAPAKPNTHSPTEERDYIPDFSQVKGQLEVKRALEIAAAGGHNILLI
                     GPPGSGKSMLAKRVPSILPDMSFDEMIETTKIHSIAGTLKHDGLITTRPFRSPHHTVT
                     PVGLGGGGTGTIRPGEVSLANNGVLFLDELPEFSRTALEVLRQPIEDGSITISRAGQK
                     CTYPCSIMVVAAMNPCPCGYYGDPTRKCTCSEQKIKRYLNRISGPLLDRFDIHVEVPA
                     VKFEELRDASSAECSADIKKRADRAREIQRERFKGSKTTCNAKINAEQFEKVCVIDKE
                     AEKTLKDAFESLGLTARAYDRVLKVARTIADLDESEIIRSEHVLEAVQYRSLDRKYWA
                     K"
     gene            3019..3213
                     /locus_tag="PLS64_01955"
     CDS             3019..3213
                     /locus_tag="PLS64_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRL42630.1"
                     /translation="MEITKNSIIGDVLDQYPDTAELFFSIGMHCLGCPASRGETIEEA
                     CMVHGADADALVADLNKAIA"
     gene            3213..3878
                     /locus_tag="PLS64_01960"
     CDS             3213..3878
                     /locus_tag="PLS64_01960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL42631.1"
                     /translation="MNGASDFSLDNRLALCADFVRDGAKVADIGTDHAYLPVWLCRIG
                     RCDTAIAADINPLPLERGIETINNSGLNNRIEARLSNGLEKISGDEADDIVIAGMGGE
                     LISQIIENWEFSKDRSKHFILQPMTKSEELMRWLFANGFSVIKRDCCTAANKCYTVIL
                     AEYSGEVRTVSESDLFLYGLDPKNNSMHRRFIEGCVRRLLKQAKGNPVCAETARILEE
                     SFK"
     gene            3875..4633
                     /locus_tag="PLS64_01965"
     CDS             3875..4633
                     /locus_tag="PLS64_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRL42632.1"
                     /translation="MKTINDILNFFEEFAPCATQMSFDNAGLIVGDKYTAVSRVLVAL
                     DITEDVVNEAESLGCELIISHHPVIFAPIKRLSPSSVPYLLAQKGISAVCMHTNLDLG
                     EKFGVNICLADALGVKNPVLSELGECMFTGELESETDIHDFAKLAKKNLDCKGLRYTD
                     IKDRVKKVAVSSGAGGSNVFDAALAGVDVLVTGEIKHHEIIAANSLGIDIIDAGHFKS
                     EDIVILPLVNKLSKEFSEIIFTKSQSCDDMIKFI"
     gene            4752..5420
                     /gene="trmB"
                     /locus_tag="PLS64_01970"
     CDS             4752..5420
                     /gene="trmB"
                     /locus_tag="PLS64_01970"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRL42633.1"
                     /translation="MRIRKKKWAEPELSVCNFYVKNPEENAGKWMQAFKKEQPLYLEI
                     GCGKGGFAGQLALKNPDKNIIALDIKVDMLGVGRRTIVKLFEDAGKTQDDITNLLLVK
                     YNVEMLDKIITADDKIDRLFINFCNPWPRAKHKKRRLTYYKKLEMYKTFLKPDSEIRF
                     KTDDDELFDESLEYFEQSGYEILYLTRDLHASDVTDNIETEHEKMFSEEGIKIKYLIA
                     RQKP"
     gene            5453..6790
                     /locus_tag="PLS64_01975"
     CDS             5453..6790
                     /locus_tag="PLS64_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42634.1"
                     /translation="MKIKKLISALVLGSMLVGLSGCNITDFSAENLLRPPKAVGDEAE
                     IEQLIAKTADSGYTLKYPKNGKFRSAIIMTDLDGNDSNEAVAFFRNSAETSSVHMLVM
                     YSKDNSWEIASDNIFETTDIDSVDFADLTGNGKAEIIVNSTTYTPNVGKLSCYSYSEG
                     KTNEVGSGQSCSSFVTGDFDNDGTSEIMSLVLYTTENEALASMLDYNEDSKSLYAKAM
                     ANMDPNVVKYKNIAVTKLDNDITGIVVDGTSANEVTNTQVIFYSRDLAILRNPLFKEK
                     AKNITRRSSQVICSDIDKDGKLEIPVTLDLPKQSGDSKNIYADKIIWNSFDTPKEALV
                     AKQAVIINYDYMFMMNYADKWSENKVTAITDLESETTSFYAWNGTKLDTELFEIKVFP
                     VSEWESGKSDDFTLITKDDKYAYTFNNINPDTQISLSDDEIKTGFSTLDQSGI"
     gene            6813..7520
                     /locus_tag="PLS64_01980"
     CDS             6813..7520
                     /locus_tag="PLS64_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL42635.1"
                     /translation="MKKILVCEDEAAIRDFVVINLTRAGYDVVEADCGEAALQKYEEN
                     NGDFDVAILDVMMPGIDGFQVCKELRNRNGGIGIIMLSAKTQEMDKVTGLMLGADDYV
                     TKPFSPSELLARVDSLYRRVALAQSHSENNFKEELKSGEFTLNLRNRALMKSGKMIEL
                     TQVEFQIMEYFFSNPGVALDRIDILNHVWGDAYVGEDKIVDVNIRRLRMKVEDEPSNP
                     KHIITVWGLGYKWDSGE"
     gene            7615..9042
                     /locus_tag="PLS64_01985"
     CDS             7615..9042
                     /locus_tag="PLS64_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL42636.1"
                     /translation="MSVILTIIILIVVSLIFIVTYIFQNSVEQNLNTTSSELSLVFSG
                     FQSDSSTSFTASARDYVENFDKKEEMEVLVINSSGRVVMTSTGFTPDDNEQIPDFNDA
                     LSNENGCAYWNGKLSSNESAMSLTRIITNENGTCVGAVRYIVSMEQVNTAIVLVSAAV
                     IACGLVIIALVILSGLMFIRSIVTPIRKLSEISSKIAHGDFSQAKKIEYKYDDEIGDL
                     CDAISDMALDLQTADQMKNDFISRVSHELRTPLTAIKGWAETMQLSERGKLDRKTFDR
                     GMGVIISESSRLTSIVEELLDFSRIQSGRMKLIKEKLDILAEFDDAVYFLKERAVTEG
                     KHLLYDEPEVVYPAVYGDKNRLKQVFLNVLDNALKYTPKGGVVAAQVIYSKDEPDIIK
                     IVITDTGCGISAEDLPKVKEKFYKANQTVGGSGIGLAVADEIMNLHNGSLDIESGEGV
                     GTTVTLTFPVYKEGSENSLPENIKL"
     gene            9084..10067
                     /locus_tag="PLS64_01990"
     CDS             9084..10067
                     /locus_tag="PLS64_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HRL42637.1"
                     /translation="MAKNTTKKITSIGGSALIEGIMMRGPKRTTVCVRTAPDKIYSED
                     ISINTIPSKCKLFKLPFLRGIAGLIDSMRLSYKALMLSADKAIESTEIEEEPSKFEKW
                     LDDKFGDKLVKVMMVIASILGVALAVGLFFFVPSFLFDLSANVVPAFKGDGGNAVFWK
                     SVFEGVLKIILFLLYIIVCSQMTEMKRVFMYHGAEHKTIFCYENEEELTVENVKKQSR
                     FHPRCGTSFMVLMLIVGILVGLFVPVAPFGIGFLRPVFKILLLPVSCGVGYELIKICG
                     KHDNAATRIIAAPGLWAQRITTKEPDDTMIEVAIEAIKAVIPEDGSDIVSK"
     gene            10067..10909
                     /gene="prmC"
                     /locus_tag="PLS64_01995"
     CDS             10067..10909
                     /gene="prmC"
                     /locus_tag="PLS64_01995"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRL42638.1"
                     /translation="MTVNEIYQSGLKLLENNNIENSKFEAQSLLQKAFSLDRVGFIIH
                     KTDKADENCSHNFLKFIEKRISGEPLQYILGEWSFMGFDFKVGRGVLIPRDDTEMVVN
                     LCIDFLKNRIDKKTVDLCSGSGAIAVALDKISGAKVTAVEIDETAFSYLETNVKENNS
                     SVKPVMADALEICDTFADGELDLIVSNPPYIKSADIETLQKEVQLEPRLALDGGEDGC
                     DFYREIVSRWSRKLKKGGALAFELGENQADAVKALMTDKGFSDFKISLDFGGVQRAII
                     GTLL"
     gene            11067..12188
                     /gene="recA"
                     /locus_tag="PLS64_02000"
     CDS             11067..12188
                     /gene="recA"
                     /locus_tag="PLS64_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRL42639.1"
                     /translation="MAVDKNKALETALTQIEKQFGKGAVMRLGENKHMNIEHISTGSL
                     SLDIALGIGGLPRGRIVEIYGPESSGKTTLSLHCIAEGQKNGGNVAFIDVEHALDPTY
                     AAALGVDVDSLLVSQPDTGEDALEIAEALIRSGAIDVIVIDSVAALVPKAEIEGEMGD
                     SHVGLHARLMSQALRKLAGAISKSNCVAIFINQLREKVGVVYGNPEVTTGGRALKFYS
                     SVRIDIRRVETLKSGGEMVGSHTRAKVVKNKIAPPFREAEFDIMYGEGISREGELLDL
                     AVKAEVVQKAGAWFSYDGRRLGQGRDKVKELLKTDKELAAEIEKKLWENIDKLYERKK
                     PAPKVKITEVPSANQEIKPDSADKTEKKSGAKIDVLVDD"
     gene            12188..12793
                     /locus_tag="PLS64_02005"
     CDS             12188..12793
                     /locus_tag="PLS64_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HRL42640.1"
                     /translation="MLITAVEPRRKAMCALYIDGEYVMNLDARTVIENRFDVGREIDD
                     EELHEIIRLSNERRAKEKALWLISYRDHSKKELTDKIRRTCDEESAEKAVERMEELGL
                     VNDENFARRYAEQLLFSKHNAPRGAVRQLVQKGIDRELAEEIIEEIDFDPCDGIRAVI
                     DRKYKNINDEKIRRRAVAALQRLGYGWDDIKTVLREYTEED"
     gene            12796..14136
                     /gene="rimO"
                     /locus_tag="PLS64_02010"
     CDS             12796..14136
                     /gene="rimO"
                     /locus_tag="PLS64_02010"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRL42641.1"
                     /translation="MNYKVGIVSLGCAKNQVDAEMLLYTLKNRGFIIVNDPADADAVI
                     VNTCGFIESAKQESINEIIELGKLKDEGKIKAIIVTGCLAQRYKSEISEQLYEVDSVI
                     GIGANETIADIVLETLDGQKCESFPDKVCLPLEGGRILSTPPYTAYLKIAEGCDNRCS
                     YCAIPMIRGRFRSRDIEDVVKEAEGLAERGVKELNVIAQDTTRFGEDKYGKPMLAELL
                     RRLCRIDGFKWIRVLYCYPDRITDELIDTIASEDKIVKYMDIPLQHCNKDILRRMNRH
                     GDRESLTALMNKIKDKIPNVIFRSTFITGFPGETEEQFNELAEFAAEMKFQRLGCFAY
                     SPEEDTKAAEMPDQIDEEIKQKRADIIMEHQQQVMAEYCESLVGKEIEVLVEGFDKLA
                     ECFFGRSYADAPEVDGCVFFTCDGEKPKAGDFVKVRVTDYTGCDPVGEFVGKID"
     gene            14190..14774
                     /gene="pgsA"
                     /locus_tag="PLS64_02015"
     CDS             14190..14774
                     /gene="pgsA"
                     /locus_tag="PLS64_02015"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HRL42642.1"
                     /translation="MNLPNKLTVGRIILVPFFVVALLANFPLNNAVALIIFIAASLTD
                     MFDGKIARKNGLVTDFGKFADPLADKILVLAAFLCFVQLGICDCVAVIIVLFREFSVT
                     SIRLIAASKGKVVAANMWGKAKTVSQMIAIIAVIAMQIVLELPEVGLNFINTATFEPI
                     FFVVGEILIWISVVFTIISGVVYIIQNRQFISQT"
     gene            14812..15465
                     /gene="cysE"
                     /locus_tag="PLS64_02020"
     CDS             14812..15465
                     /gene="cysE"
                     /locus_tag="PLS64_02020"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRL42643.1"
                     /translation="MFKTLKADIDAVMERDPAARSRAEVFFLYSGFKAVRSHRKANWC
                     LRHNLKFLARWISQRSRHKTGIEIHPGATIGKGLFIDHGMGVVIGETTIIGDNCTIYQ
                     NVTLGGTGKETGKRHPTLGNNVLVGSGAKVLGPFKVGDNARIAAGAVVLSEVPPNATA
                     VGVPARVVKVNGVRSETLDQIHVSDPVALALCNLEHKIFELQKQLDELKKENVKSEE"
     gene            15469..16875
                     /gene="cysS"
                     /locus_tag="PLS64_02025"
     CDS             15469..16875
                     /gene="cysS"
                     /locus_tag="PLS64_02025"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRL42644.1"
                     /translation="MILYNSLGQTKQEFVPHTPGKVNMYTCGPTVYHYAHIGNLRTYI
                     MEDVLEKYLRFSGYDVNRVMNITDVGHLSSDADTGEDKMLSGAKREHKTVMEIAKFYT
                     DAFFADCEKLNIKRPDVVEPATNCIDDFINMISVLLEKGYAYIAGGNVYFDTSKLDNY
                     YVFGNMNEDDLAVGVRDSVEEDENKRNKADFVLWFTKSKFDSQELKWDSPWGVGYPGW
                     HIECSCISYKHCGEYLDIHCGGIDNAFPHHTNEIAQSEAFLGHKWCPYWFHVLHLNDA
                     RGKMSKSKGGFLTVSLLEEKGYNPLVYRMFCLQSHYRKPLTFSYDNLDNTATAYNKLV
                     KRISALSRDGEPDMAKAEELTKGFSEALDNDLNTSLALTCVYDVLKADADDATKLYVI
                     GEFDKVLSLDLTKVPESNKADADDDFAKEVEELIAQRTKARAEKDWATADAIRDKLKE
                     MKVVVKDTKDGIEWHVEG"
     gene            16970..17755
                     /locus_tag="PLS64_02030"
     CDS             16970..17755
                     /locus_tag="PLS64_02030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRL42645.1"
                     /translation="MRLYIITKRNLISIGFCLLIGVLAGAIAVTSTVRAVQSAAAQRE
                     IPIYYVDTDKKQVALSFDAAWGNEQTEHLLEILDKYKVKSTFFLVGDWVKNYPDSVKD
                     IAKHGHDVGNHSNTHPHMTQMSSSDMVGQIQSCNEKIKELTGKTPTLFRAPYGDYNND
                     VVKSVNGCNMYCVQWDVDSLDWKDPTPEQIKQNIMKKIKNGSIILMHNGAKNTPEALP
                     TVIEAIKSEGYEIVPISQILLKGEYYTDVDGKMCSKTATQPSS"
BASE COUNT         5344 a         3775 c         4138 g         4647 t
ORIGIN      
        1 atcttaaaaa tataaaaaac ggtgagcaga tgcccaccgt ttttgtgttt agctttttaa
       61 atttgaaatt agtctgaaga taatcaagct tttaaaatta aaatatttaa cctcagttac
      121 gagaaattaa actaaaacaa taaaaattca attaattatt cagctgcttt tctttttgca
      181 aaacattcgc tgcaatagta gcttttgcct tcaacaggct tgaaaggaat ccttgcagtt
      241 ccgccgcatt cagcgcaaac ggtttcaaag aattctctct ctgtcttggc agcattttta
      301 cgagcctgtc tgcaagcctt gcaacgctgc ggttcgtttg tgaaaccctt ttcagcgtaa
      361 aattcctgct ctcctgcagt aaaaacaaat tcgtttccgc attctttgca gacaagtgtc
      421 ttatcttcgt acattggtat ttacctctct ttttctacct ctcggaggtt agtttgttgc
      481 cccgtgcgga gttgcaccgc aatactgtta aggacaaata ttaattttcg gttcaccgct
      541 cagccgacat tcagaccgcc gtgaaccttt ttccttgctc tttgcaaggc attgtcaacc
      601 gccttttcgg ttacggaaag cttttctgca atttccttat aactcaagcc gttgccgtag
      661 agcataagaa catcgtattc ggactttgac agcacacttt gaagtcgcaa cagcaactgg
      721 tcaagctgtt ctctgcacat caaaagttct tcgggagttt tggcggaatc ttcaatcgac
      781 tcgcccacat cgtccatcgg aacaatgctt gatgagggaa tggctttttt ctgttctccc
      841 cgtctgatta tcgaaataaa tcttctttca acaacaaccg acatatagct tttaaagctt
      901 gcaccgccgt tttggtcaaa ggctttgcag gaacaaagca gtcccacaag tccttcctga
      961 acaaagtcat tgtgttcata accctctgcc gaaaactttc gtgcaattat gctgattgtg
     1021 tcaagatatc ttacgacaag ctcatcaaaa gcacggtcat caccaagact cacaaaattt
     1081 gcaagctgtg cgtcggataa agtaccgtag gatttttgca attcggtcat aacctgcccc
     1141 taatacttat tcttatacgc ttaatagtat aacaaaaacg cacaaatgtc aacaacccga
     1201 aagcaatttc tgtaatattg cattatgata tggaaattat ttgtgcaaaa tagctgaaat
     1261 aaagtcgaaa ttacatacat tatgaaactt gttaagagaa gcagaaaatg ttatacttta
     1321 tattattaat ttattacgat ttttatttat cggttcgggg gaatttttat ggttgttacc
     1381 tgcaacagtt ttggcgtgag cggtatggac ggctacaagg ttgagcttga ggcgtcaatg
     1441 tacaacggca cacgggaatt tgatatggtc ggtctgcctg acgccgccgt gcgtgagagc
     1501 aaggacaggg ttctcagtgc attaaagaac tgcggattca gataccctgc cgcacatatg
     1561 accgtgaacc ttgcccctgc cgatatcaaa aaagaagggc ctatttacga tttgccgatt
     1621 gcggttgcaa ttcttatact gctcaatcag attaagagca acatctccga ctgcgcattc
     1681 atcggcgagc tttcgctttc gggagaagtc agaggaatca acggagttct gcccatggta
     1741 atcaaagcga aggaatgcgg tataaagaaa atttatgttc cgaaagccaa cgcaagcgag
     1801 ggcgctgttg ttgacggaat tgaagtgtat ggtattgaca acattcttca actgaaagat
     1861 tatctcaatc acattctcga acctgcaccc gcaaagccga atacacattc tcccacggag
     1921 gaacgggact acattccgga tttctctcag gtaaagggtc agcttgaggt gaagcgtgcg
     1981 cttgaaattg ccgctgccgg cggtcacaat attctgctca taggccctcc gggttcgggc
     2041 aaaagtatgc ttgcaaaacg agttccgtca attctgcccg atatgagctt tgacgaaatg
     2101 attgaaacga caaaaatcca ctcaattgca ggaacgctaa agcatgacgg acttatcaca
     2161 acacggccgt tccgttcgcc tcaccacacg gtaacgcccg taggactcgg cggaggaggt
     2221 acgggaacta tcagaccggg tgaggtttca cttgcaaaca acggtgtgct gttccttgac
     2281 gagctgcccg aattttcaag aacggcgctt gaggtactcc gtcagccgat tgaggacggc
     2341 tctatcacaa tttcaagagc aggtcagaaa tgcacctatc cgtgttcgat tatggtggtt
     2401 gccgcaatga acccatgtcc gtgcggatat tacggtgatc cgacaagaaa atgcacctgt
     2461 tcggaacaga agataaaacg ctacctcaac cgcatttcgg gaccgcttct cgaccgtttt
     2521 gatatccatg tagaagtgcc tgccgtgaaa tttgaagaac tgcgtgacgc ttcatctgcc
     2581 gaatgttcag ccgacatcaa aaagagagcc gacagagcaa gagaaattca gcgtgaaaga
     2641 tttaagggca gtaaaacaac ctgcaacgca aaaattaacg cagagcagtt tgaaaaggtc
     2701 tgcgtaattg ataaagaggc tgaaaagacg ctcaaagacg cttttgaaag tcttggactt
     2761 accgcaagag cgtatgacag ggttttgaaa gttgcaagaa ccattgccga tttggacgag
     2821 tcggaaataa tccgctccga gcatgttctt gaggcggttc aataccgcag tcttgaccgc
     2881 aaatattggg caaaataaga cctatacctt acacaaaaat taaaaatatc acttttaaaa
     2941 agtacggctt tatgctatac tactatagtg cgacatcaaa gcgatgttgc agtttcatga
     3001 cacaggaggt tttttataat ggaaattaca aagaattcaa taatcggtga tgttcttgat
     3061 cagtaccccg acacagcaga gcttttcttc agcatcggta tgcactgcct tggctgtcct
     3121 gcatcacgag gcgaaacgat tgaagaggct tgtatggtac acggtgcaga tgcagacgca
     3181 ctcgttgcag accttaacaa agcaattgca taatgaacgg cgcatctgat ttttcgcttg
     3241 ataaccgtct tgcactttgt gcggactttg tgcgtgacgg agcaaaggtt gccgatatcg
     3301 gcacagacca tgcctatctt ccggtgtggc tgtgccgtat tggcagatgc gacacagcga
     3361 ttgccgcaga catcaacccc ctgccccttg aacggggaat tgaaacaatt aacaacagcg
     3421 gtcttaacaa tcgaattgaa gcaagacttt caaacggtct tgaaaaaatt tcgggcgatg
     3481 aggctgatga catagtaatt gcaggaatgg gcggagaact catttcgcag attatcgaaa
     3541 actgggagtt ttcaaaggac aggtcaaagc actttattct tcagcctatg accaaaagtg
     3601 aagagcttat gcgttggctg tttgcaaacg gtttcagcgt tatcaagcgt gactgctgta
     3661 ctgccgcaaa caagtgctac actgtaattc ttgccgaata cagcggtgag gttcgcacgg
     3721 tatccgaaag cgaccttttt ctttacgggc ttgatccgaa aaacaactcc atgcaccgca
     3781 gatttattga aggctgtgtg cgcagacttt taaaacaggc taagggcaac cctgtctgtg
     3841 ccgaaaccgc acgcattctt gaggagagct ttaagtgaaa accataaacg atatacttaa
     3901 tttttttgaa gaatttgcgc cctgtgcaac tcagatgagt tttgacaacg cagggcttat
     3961 tgtgggcgac aaatacacag ccgtaagccg tgtgcttgtc gctcttgaca ttacggaaga
     4021 tgttgtgaat gaggcggaaa gtctgggatg tgagctgatt atcagtcatc atcccgtaat
     4081 ttttgcccct ataaaaaggc tttcgccttc ttccgttccc tatctgcttg cacaaaaggg
     4141 catttccgcc gtttgtatgc acaccaacct tgatctcgga gaaaagttcg gagtaaacat
     4201 ctgccttgca gacgcactcg gagtcaaaaa ccccgtgctg tcggaacttg gcgagtgtat
     4261 gttcacgggc gagcttgaaa gcgaaaccga tattcacgat tttgcaaagc ttgcaaagaa
     4321 aaatcttgac tgcaagggac ttcgctacac cgacattaaa gacagggtta aaaaggttgc
     4381 cgtatcgtcg ggtgcaggcg gttcaaatgt gtttgatgcg gctttggcgg gtgttgatgt
     4441 gcttgtcacg ggagaaatca agcaccatga gattatcgcc gcaaattcac tcggaattga
     4501 cattattgac gcaggtcact tcaaaagtga agatattgta attttaccgc ttgtaaacaa
     4561 gctttcaaaa gaattttccg agattatttt tacaaaatcg caatcctgtg acgatatgat
     4621 taaatttatt taacctgtaa gcatcattat caaaatattt gttgcataat ttgttacaat
     4681 ttgatataat gatacggtaa attactatat gtggtagtgc gccgtctgta cgcaccgcaa
     4741 aatgaggtat tatgagaatc agaaaaaaga aatgggcaga accggagctt tccgtttgca
     4801 atttttatgt aaagaatcca gaagaaaacg ccggaaaatg gatgcaggct ttcaaaaagg
     4861 aacagccgtt gtatcttgaa atcggctgcg gcaagggcgg ttttgcggga cagcttgcgc
     4921 ttaaaaatcc cgacaaaaac attatcgcac ttgacatcaa ggttgatatg ctcggcgtcg
     4981 gcagaagaac aattgtaaag ctttttgagg acgcaggcaa aacgcaggat gatattacaa
     5041 atcttttact tgtaaagtac aatgttgaaa tgcttgacaa aatcattacg gctgacgata
     5101 agattgacag acttttcatc aacttctgca atccgtggcc gagagcaaag cacaaaaagc
     5161 gcagacttac atactacaaa aagcttgaaa tgtacaaaac attcttgaag cctgacagcg
     5221 aaatccgttt caaaaccgat gatgacgagc tgtttgacga aagtcttgaa tactttgagc
     5281 agagcggata tgaaattttg tatctcaccc gtgatttgca cgcaagcgat gttaccgata
     5341 atattgaaac agagcatgaa aaaatgttct ccgaagaggg cataaaaatc aagtatctta
     5401 ttgcaagaca aaagccttga cagccccata aataaagacg ggagggattt ttgtgaagat
     5461 taaaaagctg atttcggctt tggttctcgg ttcaatgctt gtcggtttga gcggttgcaa
     5521 catcacggat ttcagcgcag aaaatcttct tcgtcctcct aaggcagtcg gagatgaggc
     5581 tgaaattgaa cagcttattg ccaaaacggc tgacagcggt tatacgctga aatatcctaa
     5641 aaacggaaaa ttccgcagtg caatcatcat gaccgacctt gacggaaacg acagcaacga
     5701 ggctgttgca ttttttcgca acagtgccga aacttcttcg gttcatatgc ttgtcatgta
     5761 ctcaaaggac aattcgtggg aaattgcgtc cgacaatatc tttgaaacca ccgatattga
     5821 cagcgttgac tttgcggatt taaccggcaa cggaaaggct gaaatcatcg ttaattcaac
     5881 aacctacact ccgaatgtcg gcaagctttc ctgctactcc tactcggaag gcaagacaaa
     5941 cgaagtgggt tcgggacaaa gctgttcaag ctttgttacg ggggattttg acaatgacgg
     6001 aacaagcgag attatgtcgc ttgttctcta cacaaccgaa aacgaggctc ttgcgtcaat
     6061 gcttgattac aacgaggaca gcaaaagcct gtatgccaaa gcaatggcaa acatggatcc
     6121 gaatgttgta aagtacaaga acattgccgt tacaaagctt gataacgata ttacgggaat
     6181 tgtcgttgac ggaacttccg caaatgaggt tacgaacaca caggttattt tttacagccg
     6241 tgaccttgca attctgcgta atcctctttt taaagaaaaa gcaaaaaata taacccggcg
     6301 ttcatcgcag gtcatctgtt cggacattga caaggacgga aagcttgaaa ttcccgttac
     6361 gcttgatctg ccgaaacaga gcggtgacag caaaaatatt tatgccgaca aaatcatttg
     6421 gaacagcttt gacacaccca aggaggcgct tgttgcaaaa caggcggtaa tcataaatta
     6481 cgattatatg tttatgatga actatgccga taagtggagc gaaaacaagg ttacggcaat
     6541 taccgacctt gaaagcgaaa ccacctcatt ctatgcatgg aacggcacta agcttgacac
     6601 ggagcttttt gaaataaagg tgttccccgt cagcgaatgg gaatcgggca aatcggatga
     6661 ttttacgctg attacaaaag acgataaata tgcatacaca tttaataata taaatcccga
     6721 cacacagatt tcgctaagtg atgacgaaat caagacaggt ttttccacac ttgatcagtc
     6781 gggaatctga tcacagcaac ggaggttttg atatgaaaaa aattcttgtt tgtgaggatg
     6841 aagcggcaat tcgtgacttt gtcgttatca atcttacaag ggcaggctac gatgtagttg
     6901 aggcagactg cggcgaggcg gctttgcaga aatatgaaga aaacaacggc gattttgatg
     6961 ttgcgattct tgatgttatg atgccgggta ttgacggttt tcaggtttgt aaggagctga
     7021 gaaaccgcaa cggcggaatc ggaattatta tgctttcggc aaaaactcag gaaatggaca
     7081 aggtaacagg acttatgctc ggtgcagacg actatgttac aaagcctttc tcaccgagtg
     7141 agcttcttgc aagagttgat tcactctaca gaagagttgc gcttgcacag tcgcattccg
     7201 aaaacaactt taaagaagag cttaaatcgg gcgagttcac actcaatctt cgcaaccgtg
     7261 cgcttatgaa gagcggaaag atgattgaac tcacacaggt tgaattccag attatggagt
     7321 acttcttctc gaatccgggt gttgctcttg acagaatcga cattctcaac catgtatggg
     7381 gagacgctta tgtcggcgag gataaaattg tcgatgttaa tatccgcaga ctcagaatga
     7441 aggttgagga cgaaccttca aatcctaaac atatcatcac ggtttgggga ctcggctaca
     7501 agtgggattc aggcgaataa ctttgcagaa cagcctgcat aaaatttata caatctaaag
     7561 aggagggcgg caacaatttt taagggtatt tcaaaaagat ggatgctcaa cacattgagt
     7621 gttatactga caatcataat ccttattgtt gtcagcctta ttttcattgt tacatatatt
     7681 tttcaaaaca gcgttgaaca gaacctgaac acaacaagca gcgaactttc acttgtcttt
     7741 tcgggatttc agagcgacag ttcaacctcg tttacggcgt ctgcccgtga ttatgttgaa
     7801 aactttgaca aaaaagaaga aatggaggtt cttgtaatca attcctccgg cagagttgtc
     7861 atgacttcaa caggattcac ccccgatgac aacgagcaga ttcccgattt taatgatgca
     7921 ctctcaaacg aaaacggctg tgcatattgg aacggcaagc tttcatcgaa cgaaagtgca
     7981 atgagcctga caagaatcat caccaacgaa aacggaacct gcgtaggtgc ggtaagatac
     8041 attgtttcca tggaacaggt caacacggca attgtgcttg tttctgcggc agttattgcg
     8101 tgcggtcttg ttatcatcgc ccttgtaata ttgtcgggac ttatgtttat ccgttcaatc
     8161 gttaccccga taagaaaact ttcggagatt tcaagcaaga ttgcccacgg cgatttttca
     8221 caggcaaaaa agattgagta caaatacgat gatgaaatcg gcgacctctg cgatgcaatc
     8281 agcgatatgg cgcttgattt gcagacagcc gatcagatga aaaacgattt tatttcacga
     8341 gtatcacacg agttgcgtac tccgcttacg gcgattaagg gctgggctga aacaatgcag
     8401 ctttccgaaa gaggaaagct tgaccgcaaa acctttgacc gtggtatggg agttattatc
     8461 agcgaaagct cacgccttac aagcattgta gaagagcttc ttgacttcag ccgtattcag
     8521 agcggaagaa tgaagctcat caaggaaaag cttgacattc ttgccgaatt tgacgatgcg
     8581 gtttacttct taaaggaaag agccgttaca gagggcaagc atcttcttta tgatgaaccc
     8641 gaggttgttt atcctgccgt ttacggcgat aaaaacaggc tcaagcaggt tttcctcaat
     8701 gtgcttgaca atgcgttgaa atacacacca aagggcggtg ttgttgccgc tcaggtaatc
     8761 tacagcaaag atgaacccga tattatcaaa attgtaataa ccgacacggg ttgcggaatt
     8821 tctgccgagg atcttccaaa ggttaaggaa aaattctaca aagcaaacca gactgtcggc
     8881 ggttcgggaa tcggacttgc ggttgccgat gagattatga acctccacaa cggttcactt
     8941 gatatcgaaa gcggagaggg tgtcggcaca accgtaacgc tcaccttccc tgtatataaa
     9001 gagggcagtg aaaactcact tcccgaaaac attaagcttt aatcttattt ctctgtgcag
     9061 cctaatacag aaaggaacag ctaatggcta aaaatactac taaaaaaatc acctcaatcg
     9121 gcggcagcgc cctgattgaa ggcattatga tgagaggtcc gaaacgcaca accgtgtgtg
     9181 ttcgtaccgc acccgacaag atttacagcg aggatataag cataaacaca attccctcaa
     9241 aatgtaagct tttcaagctt ccgtttttaa gaggaatcgc aggtcttatt gactcaatgc
     9301 gactttccta caaagcgctt atgctctctg ccgataaggc aattgaatca accgaaatcg
     9361 aagaagaacc gtcaaagttt gaaaaatggc ttgacgataa attcggtgac aagcttgtta
     9421 aggttatgat ggtaattgcg tcaatcctcg gtgttgcgct tgccgtggga cttttcttct
     9481 ttgtcccctc tttcctgttt gacctcagtg caaatgttgt gccggctttc aagggtgacg
     9541 gcggaaacgc tgttttctgg aagtctgttt ttgagggtgt tctcaagatt attcttttct
     9601 tactttatat tatagtatgt tcacaaatga cagaaatgaa aagggtgttt atgtaccacg
     9661 gtgccgagca caagacaatt ttctgttacg aaaacgaaga agaactcaca gttgaaaatg
     9721 ttaaaaagca gagccgtttt cacccgagat gcggaacaag ctttatggtt cttatgctga
     9781 ttgtcggcat tcttgtcggt cttttcgttc ctgttgcacc tttcggcatc ggatttttaa
     9841 gacctgtatt caagattctt cttctccccg tttcatgcgg agtcggttac gagcttatca
     9901 aaatctgcgg taagcacgac aatgccgcaa caagaatcat tgccgctccc ggactttggg
     9961 cgcagagaat cacaacaaaa gagcctgatg acactatgat tgaagttgca attgaggcta
    10021 tcaaagctgt tatccccgag gacggctcgg acattgtaag caagtaatga ctgtaaacga
    10081 aatttatcaa agcggactta aactgcttga gaataataat attgaaaatt caaaatttga
    10141 ggcgcaaagt cttttacaaa aggctttttc gcttgacaga gtcggcttta tcatacacaa
    10201 aactgataaa gccgatgaaa actgttcaca taacttttta aaatttatag aaaaacgaat
    10261 ttcaggcgag ccgttgcagt atattctcgg cgaatggagc tttatgggat tcgattttaa
    10321 ggtgggcaga ggcgtgctca ttccccgtga tgacacggaa atggttgtca atctctgcat
    10381 tgattttctc aaaaacagaa tcgacaaaaa aacggttgac ctctgttcgg gcagcggtgc
    10441 aattgccgtt gcacttgata aaatttcggg tgcaaaagtt acggcagttg aaattgacga
    10501 aaccgctttt tcatatcttg aaacaaatgt aaaggaaaac aattcgtcag taaagcctgt
    10561 tatggctgac gctctcgaaa tctgcgatac attcgcagac ggtgagcttg acctcattgt
    10621 ttcaaatccg ccttatataa aatcagccga catagaaaca cttcaaaaag aggttcagct
    10681 tgaaccaaga cttgcacttg acggcggcga ggacggctgc gatttttacc gtgaaatagt
    10741 gagccgttgg agcagaaaac tcaaaaaagg cggtgcgctt gcatttgaac tcggagaaaa
    10801 tcaggctgac gcagtaaagg cacttatgac tgacaaaggc ttttcggatt ttaaaatttc
    10861 acttgacttt ggcggtgtgc agagggcgat tattgggact ttgctctgat tttagaaatt
    10921 ttgttcgata aaattaccgc caaattattg cattttcccc tcacatattt tataatctta
    10981 gtgtgaacat aaccgccgat gaagtgacgg ttatgttaca gaccgaaaaa catcagctcc
    11041 ctttgcggag tacggaggta tatgaaatgg cagtagataa aaacaaagcg cttgaaactg
    11101 cgctcacaca aatcgaaaaa cagttcggca agggtgctgt aatgcgtctt ggcgaaaata
    11161 aacatatgaa cattgaacac atctcaacag gttcgctttc gcttgatatt gcactcggaa
    11221 tcggcggact tccccgcgga agaattgttg aaatttacgg acctgaatct tcgggtaaaa
    11281 caacactttc tctccactgc attgcagagg gacagaaaaa cggcggcaat gttgcattta
    11341 ttgatgttga gcatgcactc gatccgacat atgcggcggc tctcggtgtt gatgttgatt
    11401 cactcctcgt ttcacaaccc gacacgggtg aagacgctct tgaaattgca gaggcgctta
    11461 tccgaagcgg tgcgattgat gttatcgtaa tcgactccgt tgccgctctt gttccgaagg
    11521 ctgaaattga gggcgaaatg ggtgacagcc atgtaggtct gcacgcaaga cttatgtcac
    11581 aggcactgcg taaactcgca ggcgcaattt caaaatcaaa ctgcgttgca atcttcatca
    11641 accagttgcg tgaaaaagtc ggtgttgttt acggcaaccc cgaagtaaca accggcggtc
    11701 gtgcgctcaa gttctacagc tccgttcgta ttgatataag aagagttgaa acacttaaat
    11761 caggcggtga aatggtcggt tcacacacaa gagcaaaggt tgtaaaaaac aagattgctc
    11821 ctccgttccg tgaagctgaa tttgatatta tgtacggtga aggcatttcc cgtgagggcg
    11881 aacttcttga ccttgccgta aaggctgagg ttgttcaaaa agcaggagca tggttcagct
    11941 atgacggcag acgactcgga cagggacgcg acaaggtcaa ggaacttctc aaaaccgaca
    12001 aggagcttgc tgctgaaatt gagaagaaac tctgggaaaa tattgacaag ctttatgaac
    12061 gcaaaaaacc tgctccaaag gtaaaaatta ccgaagttcc gtctgcaaac caagaaatca
    12121 agcctgattc tgcggataag acagagaaaa agagcggtgc aaaaattgat gttctggttg
    12181 acgactgatg cttataaccg cagttgaacc acgaagaaag gcgatgtgtg cgctctacat
    12241 tgacggcgaa tatgttatga atcttgacgc acgcacagtt attgaaaacc gatttgatgt
    12301 cggcagagag attgatgacg aggagctgca cgagattatc aggctaagca atgagcgccg
    12361 tgcaaaggaa aaggctttgt ggcttatttc ctatcgtgac cactcaaaaa aagaactcac
    12421 cgacaaaatc agacgcacct gtgacgagga atcggcggaa aaggctgttg agcgaatgga
    12481 agaactcggt cttgtaaatg acgaaaactt tgcaagacga tatgccgaac agcttttgtt
    12541 ttcaaaacat aatgctccga gaggtgcggt aagacagctt gttcaaaagg gaattgaccg
    12601 tgaacttgca gaagagatta tcgaagaaat cgactttgat ccgtgtgacg gaataagggc
    12661 ggttatcgac agaaaatata aaaacataaa tgatgaaaaa ataaggcgaa gagccgttgc
    12721 cgctctgcaa aggctcggat acggatggga tgacattaag accgtcctca gagaatacac
    12781 agaggaagac tgattatgaa ttataaagta ggcattgtat cactcggttg tgcaaaaaat
    12841 caggttgacg ccgagatgct tttgtacaca ctcaagaaca gaggatttat tattgtgaac
    12901 gatccggcag atgcggacgc agtaattgta aacacctgcg gatttatcga aagtgcaaag
    12961 caggaaagca ttaacgaaat tatcgaactc ggcaagctca aggacgaggg caaaatcaag
    13021 gcaatcatcg taacaggctg tcttgctcaa agatataagt cggaaatttc cgaacagctt
    13081 tacgaggttg actcggttat cggaatcggt gctaacgaaa ctatcgcaga catcgttttg
    13141 gaaacacttg acggacagaa gtgcgaaagc tttcccgaca aggtttgtct gccgcttgag
    13201 ggcggaagaa ttctttcaac tcccccctac accgcttatc tcaaaattgc cgagggttgt
    13261 gacaaccgtt gttcctactg tgcaatcccg atgattcgag gaagattccg cagcagagat
    13321 attgaagatg ttgtaaaaga ggcggaaggt cttgctgagc gtggagtcaa ggaactcaat
    13381 gtcattgctc aggacacaac ccgtttcggt gaggataaat acggcaaacc gatgcttgca
    13441 gagcttctca gaagactttg taggattgac ggctttaaat ggataagggt tctctactgc
    13501 tatcccgaca gaattacaga cgagcttatt gacacaattg caagtgaaga taagattgta
    13561 aaatatatgg atattccact ccagcattgt aacaaggata ttctccgcag aatgaatcgt
    13621 cacggcgaca gagaaagcct cactgcactc atgaacaaga ttaaggataa aattccgaat
    13681 gtaatcttcc gctcaacctt tattacaggt ttccccggtg aaactgagga gcagttcaat
    13741 gagcttgcag agtttgccgc tgagatgaag tttcagcgac tcggctgttt tgcctactct
    13801 cccgaggagg atacaaaggc ggctgaaatg cccgaccaga ttgacgaaga aatcaagcaa
    13861 aagcgtgccg atattattat ggagcatcaa cagcaggtta tggctgaata ctgcgaaagc
    13921 cttgttggca aagagattga agttcttgtt gaaggctttg acaagcttgc cgaatgtttc
    13981 ttcggcagaa gctatgccga cgcccccgag gttgacggct gtgtattctt cacctgtgac
    14041 ggtgaaaagc caaaggcagg agattttgtc aaggtgcgtg taaccgatta cacaggctgc
    14101 gatcctgtag gcgaatttgt cggcaaaatc gactaagtgg ctaaaagact cagcaatttc
    14161 ataatattta actttgaaag gcacataaaa tgaacttacc aaataaactg acagtaggac
    14221 gaataattct tgttccgttc tttgtggtgg cattgcttgc aaactttccg ctcaacaatg
    14281 ccgttgccct gattattttt attgcggcgt cactcacaga tatgtttgac ggaaaaattg
    14341 caagaaaaaa cggtcttgtt acagacttcg gaaaatttgc agatccgctt gcagacaaaa
    14401 ttcttgtgct tgccgcattt ctgtgctttg tacagcttgg aatatgtgac tgcgttgcgg
    14461 tcataatcgt gcttttccgt gaattttccg tcacctcaat acggctgatt gccgcctcaa
    14521 aaggcaaggt tgtcgccgct aatatgtggg gcaaagcaaa gacggtttca cagatgattg
    14581 ctataattgc agtaattgca atgcaaatcg tgcttgaact gcccgaagtc ggacttaatt
    14641 ttataaacac ggcaacattt gaaccgattt tctttgtggt gggagaaatt ctcatctgga
    14701 tttcggttgt attcacaatc atttcgggcg ttgtatatat aattcaaaac agacagttta
    14761 tttctcagac ctgagaacag actctcacgg tcttgacagg agggtgattg cgtgtttaaa
    14821 accctgaaag cggatattga tgcggttatg gagcgtgatc ctgccgcaag gagcagagca
    14881 gaggtgtttt ttctctactc gggtttcaag gcggtgcgtt ctcaccgaaa agccaactgg
    14941 tgtttgcgac acaacctcaa gtttcttgca aggtggattt ctcaacgcag tcgtcacaag
    15001 acgggaattg agattcaccc gggtgcaacc atcggcaagg gactgtttat agatcacgga
    15061 atgggcgttg tcataggcga aactacaata ataggagaca actgcaccat ttatcagaat
    15121 gttactctcg gaggaacagg caaagaaacg ggaaaaagac accccacact cggcaacaat
    15181 gtgcttgtcg gctcgggtgc aaaggttctc ggtccgttca aggttggtga caacgcaaga
    15241 attgccgcag gtgcggttgt tttaagcgaa gttccgccga atgcaacggc tgtcggcgtt
    15301 ccggcaagag tagtaaaagt caacggtgtt cgcagtgaaa cccttgacca gattcatgta
    15361 tcggatccgg ttgcacttgc attgtgcaat cttgagcata aaatttttga acttcaaaaa
    15421 cagcttgatg agctgaaaaa ggaaaatgta aaaagtgagg aatagcaaat gattttatat
    15481 aattctctcg gacagacaaa gcaggaattt gtaccgcaca cacccggcaa ggttaatatg
    15541 tacacctgcg gtcctactgt ttatcactat gcccacatcg gcaatcttcg cacctatatt
    15601 atggaagatg ttcttgaaaa gtatcttcgt ttttcgggtt atgatgtaaa ccgtgtaatg
    15661 aacatcacag atgtaggtca tctttcaagc gatgccgaca caggcgagga taagatgctt
    15721 tcaggcgcaa agcgtgagca taaaactgtt atggagattg caaagttcta cacagacgca
    15781 ttttttgccg actgcgaaaa gctcaacatc aagcgtcccg atgttgtaga gcctgcaaca
    15841 aactgcattg acgattttat taatatgatt tccgttctgc ttgaaaaggg ctatgcctac
    15901 attgcaggcg gcaatgttta ctttgacact tcaaagcttg acaactacta tgtgttcggc
    15961 aatatgaacg aggatgacct tgctgtcggt gttcgtgaca gcgttgaaga ggacgaaaac
    16021 aagcgtaaca aggctgactt tgttctctgg ttcacaaagt caaagtttga ttcacaggag
    16081 ctcaagtggg acagtccgtg gggcgtaggt tatccgggct ggcacatcga atgttcatgc
    16141 atcagctata agcattgcgg tgaatatctt gacattcact gcggcggcat tgacaatgct
    16201 ttcccccatc acacaaacga aattgcacag agcgaggctt ttctcggtca caagtggtgt
    16261 ccgtactggt tccatgtact tcatctcaat gatgcaagag gcaagatgag caagtcaaag
    16321 ggcggtttcc ttacagtttc acttcttgaa gaaaagggct acaatccgct tgtttacaga
    16381 atgttctgcc tccagagcca ctaccgcaag ccgctcacat tctcatatga caaccttgac
    16441 aacacagcca cagcctacaa caagcttgta aagagaattt ccgctctttc aagggacggt
    16501 gagcctgata tggcaaaggc tgaggagctt acaaagggat ttagcgaggc gcttgacaat
    16561 gacctcaaca cttctcttgc tctcacctgc gtttatgatg ttctcaaggc tgacgcagac
    16621 gatgcaacaa agctttatgt aatcggcgaa tttgacaagg tgctttcact cgaccttaca
    16681 aaagttcccg aaagcaacaa agcggacgct gacgatgact ttgccaagga agttgaggag
    16741 cttattgcac agcgtacaaa agcccgtgcc gaaaaggatt gggcgacagc cgacgctatc
    16801 cgtgacaagc ttaaagagat gaaagttgtt gtcaaggaca caaaagacgg cattgaatgg
    16861 catgttgagg gttaacttga ccgctatcgc ccaatgaacg cacctaatta aaacaaaata
    16921 tgaataaata aaaatcccct tcatataaat ttggtatgga ggggatttta tgcgtttgta
    16981 tatcataaca aaacgaaatc taatatcaat cggattctgt cttttaatcg gagtgcttgc
    17041 aggggcaatt gcagtcacat caaccgttcg agccgttcag agtgcggctg ctcagcgtga
    17101 aattcccatt tactatgttg acacggacaa aaagcaggtt gccctgtcgt ttgatgcggc
    17161 atggggcaat gaacagaccg agcatctgct tgaaattctc gacaaataca aggtgaaatc
    17221 aacatttttc cttgtgggcg actgggttaa gaattatccc gacagcgtaa aagatattgc
    17281 aaagcacggt cacgatgtgg gcaatcacag caacacccat ccgcatatga cgcagatgtc
    17341 atcgtccgat atggtcggtc agattcagtc ctgcaacgaa aaaatcaagg agcttacggg
    17401 caaaactccc accctgttcc gagcccccta cggcgattac aacaatgatg ttgtaaagtc
    17461 ggtcaacggc tgtaatatgt attgcgtgca atgggatgtc gattcgctcg actggaaaga
    17521 tcctactccc gagcagataa agcagaatat tatgaaaaaa atcaagaacg gcagtattat
    17581 attgatgcat aacggtgcaa aaaatacccc cgaagcattg ccgacggtaa tcgaggcaat
    17641 caagtccgag ggctacgaaa tcgttccgat ttcgcaaatt ctgctcaaag gcgaatacta
    17701 cacagatgtt gacggcaaaa tgtgcagtaa aaccgccaca caaccgagtt cgtgagtaaa
    17761 gaatatttat aaataccgct acggataata cacaactttc attacatcaa aacattttag
    17821 taaaacaaat catttcatca aagcgggcga acgaggttcg cccgctacaa aatagcaata
    17881 acattcatac aaaaacagac tgac
//
LOCUS       DAOMVE010000018        17664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_618_length_17664_cov_4.037594, whole genome shotgun sequence.
ACCESSION   DAOMVE010000018 DAOMVE010000000
VERSION     DAOMVE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17664
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_618_length_17664_cov_4.037594"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1619)
                     /locus_tag="PLS64_02035"
     CDS             complement(84..1619)
                     /locus_tag="PLS64_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL42646.1"
                     /translation="MPNWLNDAVFYEIYPQSFCDTNGDGIGDIEGIISKLDYVKSLGC
                     NAIWLNPIYDSPFMDAGYDVRNYKKVAERYGTNDDLVRLFDEAHKKGIKILLDLVPGH
                     TSDQHEWFLEAKKAAKSEYSNRYIFTKSVWDAPPQYRMMCGLCERDGNYMVNYFSSQP
                     ALNYGFHEITHPEWQLPCDHPDCLATAEAMKDVMRFWLDKGADGFRVDMADSLVKNDD
                     DKIATAKIWRGVRDMLDTEYPNAAMVAEWCNPERAINNAGFHMDFYLDHYGNGYSRLF
                     RYGEFGDQAVFNPNGKGDIKAFADEYLPNYEKTKDNGYISFMTNNHDMPRVTAYLDKE
                     AIKLVNAFIFTMPGVPFLYYGDEIGMRYQKGIVSKEGGYSRTGSRTPMQWNSGKNLGF
                     STSDEPYLAVDKSADAPTVENQKDDPDSIYKVVTDIIALRHKYDDLKGNGELEFMYEE
                     GKIPFAYKRGNLVMYFNPLGESAVMNAKYTGKTVYALGNAEFANGKVTMSPQSFALVE
                     IDG"
     gene            1848..2402
                     /locus_tag="PLS64_02040"
     CDS             1848..2402
                     /locus_tag="PLS64_02040"
                     /inference="COORDINATES: protein motif:HMM:NF017226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HRL42647.1"
                     /translation="MTVDKLGDNKLLIKLDESDMENFELDFGKMSFKNEDNQKVIIGL
                     MRLACCRAGLDTNGKNVKIEAFLLGDECCILVTANPKKRKYRLKKSDSGLCWKMANAS
                     DFLNAVEALYRSNICCRKSSAYEMSGNYYLVFDYFSLPKHMIRVLSEYGERQKSRNAA
                     ARVRENANLLCHADAVAVIGKFLV"
     gene            complement(2499..2675)
                     /locus_tag="PLS64_02045"
     CDS             complement(2499..2675)
                     /locus_tag="PLS64_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HRL42648.1"
                     /translation="MDDNILRYTLRVNRTLFQKFRYVADYEGRSANREIEQYIKQRVK
                     SFEEEHGEIEVKKD"
     gene            2790..3128
                     /locus_tag="PLS64_02050"
     CDS             2790..3128
                     /locus_tag="PLS64_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HRL42649.1"
                     /translation="MKIAIVGSRSITVNNLGDYLPENTTEIVSGGAIGVDRSARNYAK
                     THNIKLKEFLPEYERYGRSAPLKRNLQIIDYADEVIAFWDGMSHGTRFVIENCKRKNV
                     PIKVYALANK"
     gene            complement(3252..3629)
                     /locus_tag="PLS64_02055"
     CDS             complement(3252..3629)
                     /locus_tag="PLS64_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42650.1"
                     /translation="MKVRVMCLVLCVFVFASIFCGCGSNGNTEIIGKWVPTTAKTDGE
                     TISYSELGIDEDQFGLDFKDSSNCTVTLAGISCDSAYTFNGTSVDLTVNGTAYKLSYD
                     SGVLTMVLNYDNNRIEFSFTKQR"
     gene            3838..6531
                     /locus_tag="PLS64_02060"
     CDS             3838..6531
                     /locus_tag="PLS64_02060"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRL42651.1"
                     /translation="MAKELAKSYNPSEFEDRIYDFWLKGNYFHAEVDKDKKPYTIVMP
                     PPNITGQLHMGHALDETLQDILIRWRRMQGYSALWLPGTDHASIATEAKIVEAMRKEG
                     ITKEDIGREEFLKRAWEWKKVYGGRITSQLKKLGSSCDWERERFTMDEGCSKAVKEVF
                     VRLYNDGKIYRGNRMVNWCPHCCTSISDAEVEYKEQNGHFWHLLYQVKETGEYLEIAT
                     TRPETMLGDTAVAVNKDDERYKHLHGCHVILPLLNKEIPIVCDEHADMEKGTGVVKIT
                     PAHDPNDFEVGQRCNLPIVRCFTYDGHLTGAKDVEEYNELKAKGQLAENEPEVLDCGK
                     YAGMTTMEAREAIVADLKEIGALKEVEELTHDVGTCYRCHTTIEPMVSKQWFVKMAPL
                     AKPAIDVVRKGDTKFIPERFEKVYFHWMENIKDWCISRQLWWGHRIPAWYCDDCGEVM
                     VAKDAPEKCCKCGSTHIHQDEDTLDTWFSSALWPFSTLGWPDKTPELEYFYPTSTLVT
                     GYDIIFFWVARMIFSACENMGSAPFKTVFMHGIVRDENGIKMSKSLGNGIDPLEVIDK
                     YGADALRFFLATGNTPGNDMRYSDKRVEACANFANKLWNASRYVHMNIDDHDVKNELP
                     KTLTTEDKWIVSTLNTVAKEVNENLEKFELGIAVQKIYEFIWDCYCDWYIELVKTRLS
                     SDGEDAQNARQVLLYVLDQILRLLHPFMPYITEEIWQTIPHEGETVMLAKYPEYNAEL
                     DYPEASDEMKKIMDAIRAIRNRRAEMNVPPSRKAKVYVATKFADTFRDGTQFIQKLAS
                     ASEVEVAESFEIEGAVNIVTADAKIYIPMDELVDREKELARLNKELEQVKKRLAQSEG
                     KLNNQGFVAKAPEAVIEKVKGQAAKEREQIALIEAAIAALK"
     gene            7264..8364
                     /locus_tag="PLS64_02065"
     CDS             7264..8364
                     /locus_tag="PLS64_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HRL42652.1"
                     /translation="MHALKRFGTAFGGYKMMENYPVPECGPDDIILEIKAAAICGADM
                     KHWGVDNGYDDTNITPDQQQSVRGHEFAGEIVKVGENVKDWHIGQRVVSDNSAHVCGV
                     CPACEQGDFLCCEHKVNLGLDNNTWGGGFSKYCKVPGEILAIHKHAIWEIPEGIKYEE
                     ACVLDPICNAYKAVAQQSHLIPGQDVVVFGTGPLGLFSVQVAKLMGAVNIVMVGLDDD
                     VKVRFGVAKELGATHCVNGSKEDVVARCTEICGRDNLGLVIECSGANIALKQAIEMTR
                     PNAEIVRVGMGFKPLEFSINDITAWNKSIIGHMAYDSTSWRNCIRLLQSGQIKVQPMI
                     THRLGLSQWQEGFDKMAKKEAIKVIFHYDYED"
     gene            8398..9099
                     /locus_tag="PLS64_02070"
     CDS             8398..9099
                     /locus_tag="PLS64_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose phosphate epimerase"
                     /protein_id="HRL42653.1"
                     /translation="MRKLMIAPSVGCCDLFHVEEQVKLINEKSDYLHMDIKDGVYVPS
                     YGIGPDYLDYLNKHVENLKPMDAHLMVKHPQQYLETFAKAGAAYITPHTDCIEGDAFV
                     TIHKIKELGCKAGVALSPSVPLSTIEYYLPLLDKVTIMIVDPGIAGQPVDPQMFVKIN
                     QLAKIRKERGLNFLIEADGSMNSSLYRPLYEAGADLVILGPPALWNKDTDFNKAWDIM
                     ESELERELDGAERKI"
     gene            9142..10530
                     /locus_tag="PLS64_02075"
     CDS             9142..10530
                     /locus_tag="PLS64_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL42654.1"
                     /translation="MANKTSVNIGARLDRLPQSKWHVKIWLVTAFALLVCWSNGIGGS
                     VQNILLNELHWLEPGSTLLAMWGTTYTAGQLFGALIGGPIGDKIGRKKSILLYEIIHI
                     IAMIGGALSPNIAVLYVFRLLQGLALGALLVVLFAGFTEYVPGKNRGTWSSRTSFIGN
                     WAHPICNGIALLIVSTGVSMNMNWRIQFMIPSILSIIACIFIYVKFPESPRWLESQGR
                     LDEADKIMTSIEKEIEKSTGKPLPPVEVTNPKPVKKLPFTALFKGKLLRRTIVGSLVL
                     IGMNTIQYTLMNWMPSMLSGLGYDTSKSQFMTMFGLFGAPFGIFIASLIMDKIPRRVM
                     GVILLAGMAGLGVATGLLAPTLGMTGLIVMTFLLNTIIYMYVCYASAVYVPEMWPTSA
                     KLSGSGFSNAMGRVSNIFFPFLVTNVAASSMGSGGVFILIAIVAAIIAVAVIFLGVET
                     RGESVENIGDVE"
     gene            10788..11843
                     /locus_tag="PLS64_02080"
     CDS             10788..11843
                     /locus_tag="PLS64_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRL42655.1"
                     /translation="MKNSEAILVTPKQFEIQECPVPEPKDHEILMKVEYVGMCGSDIH
                     GFEFGPFIPPKDPNQKIGLGHEVAGEVVKVGSKVTRFKAGDKVLIEPGVPDDKCEYCR
                     TGRYNICPDVDFMATQPNYKGALTQYMTHPEEWTYHIPEGMTTMEAALVEPAAVGMHA
                     AILGGAQLGKSIVILGGGTIGLMVLQACKSLGATDITVVDIMQNRLDLALKLGASRVI
                     NGKEQDTVALLRSPEYFGDHGCELVFEAAGSVFTAQQAVQIVARGGKIMMVGTQSKPV
                     PIDFLKINREVMIQTSFRYCNNFPQTIEAIASGKFNVKDMVTNIYDYKDVQQAFMDAI
                     DPEKKANMVKGVIKVAD"
     gene            11889..12728
                     /locus_tag="PLS64_02085"
     CDS             11889..12728
                     /locus_tag="PLS64_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HRL42656.1"
                     /translation="MLSDIRYWEKKATEGHYAIPHFNVWNAEMLMGVIDAAEELRAPI
                     IISFGTGFTGNTSFEDFCYMMDSMAKKATVPVIEHWDHGRNMTILQNAVNHCMNSVMR
                     DASALPYEENVAEIKRCVDYFHAYNIPVEAELGHVGNETVYEEALSEYQYTDPSKAKE
                     FVERTGCDSLAVAIGNVHGVYSSEPKLAFDVLEAVAKEVDVPLVLHGASGIGDEDIKK
                     AISLGIAKINIHTELCQAAMEAINAHKDEPFLAVEREVRKAVKERAMYKIKLFGDDGR
                     ADE"
     gene            12730..13701
                     /locus_tag="PLS64_02090"
     CDS             12730..13701
                     /locus_tag="PLS64_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HRL42657.1"
                     /translation="MSNTEKKLDVICLGAAVVDIPLRPVSRDIFDIESYPVDRIAMSV
                     GGDAMNEATIISRLGFKTGIIACIGDDAAGQFILRSCEKDNIDVEGIKIDPTIDTSMN
                     IGLVTDDGERTFVTNRNGSLWKENIEHVDFEKMKQAKILSLASIFNCPLLDGKTLVKI
                     FKEAKAAGMTITADMIKPRLGETLDDIREALSYVDYFFPNFDEACLMTGETEESKVAD
                     KLFECGVKNVIMKIGKRGCYIRNAAGAMIVPACKGVTAIDTIGAGDNFASGFISALLQ
                     GKDIRDCGIYANCTAAISVQYVGATTGVRNKEMVEEMLEKYKKDYIL"
     gene            13793..14785
                     /locus_tag="PLS64_02095"
     CDS             13793..14785
                     /locus_tag="PLS64_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRL42658.1"
                     /translation="MKTMKLGKTGIDISRMGLGTWSIGGGSAWGGDHDLQVVVDTIRE
                     APKLGVNLIDTAPGYNFGNSEKILGKALEGMNREDVVIITKCGVTWDQEMKGDLFNKV
                     NGIQLYKNLNSASIKREIEESLKRMGTDYVDVYMTHWQSIEGSEYYVPIQKTMEVLND
                     LKAQGKIKAIGAANVDIKQIQEYLKWGELDIVQAKYSILDRGIEDEIIPCCRENGVTI
                     QAYSPLEMGLLSGTFPRDYKPVGAQIPKKWFQPENMQKAMDVMDQWKPLCEKYNCTIA
                     NLALAWILAQGDFINLLSGSTTVDQIAENVKSAELELDSADVAMMRDMVEAIDK"
     gene            14927..15679
                     /locus_tag="PLS64_02100"
     CDS             14927..15679
                     /locus_tag="PLS64_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRL42659.1"
                     /translation="MAKRIDEIRQKVETEGEVFVSDLSRIYNVTEETIRRDLEKLKND
                     GIITRTFGGAVLNTTPQNDRVHFFKRKGINLESKKKIAKLLVDKFIGVNTMMIDNSTT
                     VLEAVKLFKDNENLTAITFSAQIFQELDTCKMKLVSTGGLYDEKTRSFYGKPAKEAMK
                     KYNVDLVLLGCKGLDMERGITDSSEGEADLKSEMASRAECVVLLADHSKFNHTAFVNF
                     LDWDRIDYLITDEKPSQEWLEFCEQMKIKLMY"
     gene            16083..17453
                     /locus_tag="PLS64_02105"
     CDS             16083..17453
                     /locus_tag="PLS64_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HRL42660.1"
                     /translation="MTKQKEKKKASIVKKMMIAVVAGFVVGFICLLIKSAVMGTDGEG
                     AWKVVEAILFQDITATKDIEGLGLFYIVGQLFMKGLQMMIVPLVLCSLTLALCSLANP
                     KKLGRIAGKTFVSYLCFYIVAAALAGAAAYLAKSLGWFTVNLPAQQAQDIVTMEGYNP
                     LVTIVNAVPSNMINAMSSNNTILCVVVIAIIFGLCMTKMGEKADPLKKVFENINDIVQ
                     MFLNFLINKIAPIAIFCMIVRALAVYGIEYISPTMMWIVVTIVVSLLLVCTIYPIGIF
                     ITTGLNPFIFLKKAAKIGMFAAATNSSAATLPLNKETCINELGCSEEISSFVLPTGMT
                     INMNGTTAMHMIAITFIATAAGVNITPATLCLTAFLSICTAVGTPAIPVAGTTMVYVV
                     MMGLGLNSDLCMIGYSLILAMNYLPGMAVITLNVIGDAATNVIVCAKEHCLNKEIYNS
                     KTPKNN"
BASE COUNT         5267 a         3612 c         3953 g         4832 t
ORIGIN      
        1 ttataataaa attcaactta aaacaccctt tcggtgaatt ccgaaagggt gtttttatgc
       61 ttaaactata aaattatatt taatcagccg tcaatttcta cgagggcgaa gctttgcggt
      121 gacattgtta ccttgccgtt tgcaaattca gcgttgccga gggcatatac tgttttgcct
      181 gtatattttg cgttcattac tgcactttca ccgagggggt tgaagtacat aacgaggttg
      241 cctctcttgt atgcaaacgg aattttgcct tcttcataca taaattcaag ctcaccgttg
      301 cctttaaggt catcgtactt gtgacgaagt gcaatgatgt ctgttacaac cttgtagatg
      361 ctgtcgggat cgtccttctg attctcaaca gtcggagcgt cggcactctt atcaactgca
      421 agatacggct cgtcacttgt tgagaaaccg aggttcttac cgctgttcca ctgcatagga
      481 gtacgggagc ctgttcttga gtaaccgccc tccttgctta cgatgccttt ctgatatctc
      541 atgccgattt catcaccgta gtagaggaac ggaacacccg gcattgtgaa aatgaatgca
      601 ttcacaagct tgatagcctc tttgtcaaga tatgccgtaa ctctcggcat atcgtggttg
      661 tttgtcataa agctgatgta gccgttgtcc tttgtctttt catagttagg aagatattcg
      721 tctgcaaatg ctttgatatc acccttgccg ttaggattga aaacagcctg atcaccgaat
      781 tcaccgtagc ggaaaagtct gctgtagccg tttccgtagt ggtcaagata gaagtccatg
      841 tggaaacctg cattgttgat tgctctttcg ggattgcacc actctgcaac cattgcggca
      901 ttcggatatt ccgtgtcgag catatcacgc acaccacgcc agatttttgc cgttgcaatt
      961 ttatcgtcat cgttcttaac aagtgagtca gccatgtcaa ctctgaaacc gtcagcaccc
     1021 ttgtcaagcc aaaaacgcat aacatccttc atagcctctg ccgttgcaag acagtcggga
     1081 tgatcacacg gcaactgcca ttcgggatgt gtgatttcgt ggaaaccgta gttaagagca
     1141 ggctgtgagc tgaagtagtt taccatatag ttgccgtcac gctcgcaaag tccgcacatc
     1201 atacggtact gcggcggtgc gtcccatacg ctctttgtaa agatgtaacg gtttgaatat
     1261 tcgctctttg cggctttctt agcctccaag aaccattcgt gctggtctga tgtgtgaccg
     1321 ggaacgaggt caagaagaat cttaatgccc tttttgtgag cctcgtcaaa aagacgaaca
     1381 aggtcatcgt ttgtgccgta acgctctgca accttcttgt agttgcgaac atcatagcct
     1441 gcatccataa aaggtgaatc gtaaatcggg ttgagccaga ttgcgttgca accgaggctt
     1501 tttacatagt caagcttgct gatgataccc tcgatgtcac cgattccgtc accgttggtg
     1561 tcacaaaaac tctgcggata aatttcatag aaaacagcgt cattaagcca gtttggcata
     1621 aaaatcattc cttttgcttt ttattttctg tggctgtatc ttacggcatc attgtagcat
     1681 taaattgtta tgttggcaac ctttttgtat tgcatacaca aagcctgaca attatgtgtg
     1741 aaaaaacagc caaattttat tccattaata tttacttgca aaagtgcttc gttcatcata
     1801 atatgtaaac agatgtatgt gtatcggaat ttaggggagg gagtattttg actgttgaca
     1861 aactcggcga taataaactg ctgattaagc ttgatgaatc ggatatggag aattttgagc
     1921 ttgatttcgg aaaaatgagc tttaaaaacg aggataatca aaaggtgatt atcgggctta
     1981 tgcgacttgc ctgttgcagg gcgggacttg acacgaacgg taaaaatgtg aaaatagagg
     2041 cttttctgct cggcgatgaa tgttgtatcc ttgttacggc aaatccgaaa aaacgaaaat
     2101 acaggctgaa aaagtccgac agcggtctgt gctggaaaat ggcgaatgcg tcggattttc
     2161 tgaatgcggt agaggctttg taccgcagta acatatgttg cagaaaaagc tccgcatacg
     2221 aaatgtcggg aaattattac cttgtgtttg attatttttc attgccgaag catatgatac
     2281 gggtgttgtc ggaatacggt gaaagacaaa aatcccgcaa cgcagcggca agagtgcgtg
     2341 aaaacgcaaa cctgttgtgt catgccgatg cagttgcggt gataggaaag tttcttgtgt
     2401 aaattgaaaa tggaaaattg aaagttgaaa attgttttgt ttgattaaaa aacacccttt
     2461 cggaaatact ccgaaagggt gttaatctat gcgtatgttt aatctttttt tacttcaatt
     2521 tcgccgtgtt cttcttcaaa ggacttaacc ctttgcttta tatactgctc aatttcacga
     2581 tttgcggaac gaccttcata gtcggcaaca tatctgaatt tctgaaaaag cgtgcggtta
     2641 actctcagag tgtatcgtaa aatattatca tccataatcg gtactcctaa atgttattat
     2701 gacactattt taatgtaatt attgcgtcaa aacgaaaatg gtgctattat gatgtcataa
     2761 tggtgtcata attttaggag tgtgaaaaaa tgaaaattgc gatagtaggc tcaagaagta
     2821 ttacagtgaa taatttaggc gattatttac ctgagaatac aactgaaata gtaagcggtg
     2881 gtgctattgg tgttgacaga agtgcgagaa attacgctaa gacacataat attaaactaa
     2941 aagaattttt acccgaatac gaacgatacg ggcggtcagc acctttgaaa agaaatttgc
     3001 aaataattga ttatgccgat gaagttattg cattttggga cggtatgtca cacggaacac
     3061 ggtttgttat agagaattgc aaaagaaaaa atgtgccgat taaggtttat gctttggcaa
     3121 ataagtaatg ttgcaaacga accgaaaatt tcatatataa aaaacggtcg agcaacatag
     3181 ctatgcgttt tgcaaagcaa ctgcccgacc gaaaattttt attttacatt ttcaattttc
     3241 aatttgtaaa actatctttg ttttgtgaat gaaaactcaa ttctgttgtt gtcataatta
     3301 agcaccattg tcagcacacc gctgtcatag ctcagcttgt atgccgtacc gtttaccgtc
     3361 aggtcaacgg aggttccgtt gaatgtgtag gctgaatcac acgaaattcc tgcaagggta
     3421 accgtgcagt tactgctgtc cttgaaatca agaccaaact gatcctcgtc aattccaagc
     3481 tcactgtatg aaattgtttc gccgtctgtc tttgctgttg tcggtaccca tttgccgatt
     3541 atttcggtat ttccgttgct tccgcatccg cagaaaattg atgcgaaaac aaacacgcac
     3601 agcacaaggc acataaccct gactttcata taatacacac ccttgaattt gtttgttcct
     3661 cttatgcagt atattttacc attttacgct ttattcgtca aactttaaat gttataaaat
     3721 tgaaattata tgccacattt tatttgcgga taattgacaa atcgacacat aaatgagata
     3781 ataaaaaggt atatgaactc ggtttatccg aacaataaat caagaggagt aatgactatg
     3841 gcaaaggaac ttgctaaatc ctacaacccg tcagagtttg aggacagaat ttacgacttc
     3901 tggctgaaag gtaactactt tcatgcagag gttgacaaag ataaaaaacc ttatacaatc
     3961 gtaatgccgc cgccaaacat cacaggtcag ctgcatatgg gacacgcact tgacgaaaca
     4021 cttcaggaca ttcttatccg ttggagaaga atgcagggct acagcgcact ttggctcccc
     4081 ggtacagacc atgcgtcaat cgctacagag gctaaaatcg ttgaggcaat gcgtaaagag
     4141 ggtatcacta aagaggatat cggcagagaa gaattcttaa agcgtgcatg ggaatggaaa
     4201 aaggtgtacg gcggaagaat cacaagtcag cttaaaaagc tcggttcaag ctgtgactgg
     4261 gagcgtgagc gtttcacaat ggacgagggc tgttcaaaag ctgttaaaga agtttttgtt
     4321 cgcctttata atgacggaaa aatctaccgt ggcaaccgta tggtaaactg gtgtcctcat
     4381 tgctgtacat caatttcgga tgccgaggtt gaatacaagg agcagaacgg tcatttctgg
     4441 cacttgctct atcaggttaa ggagacaggc gagtaccttg aaatcgcaac aacccgtccc
     4501 gaaacaatgc tcggtgatac tgctgttgcc gtcaacaagg acgatgagcg ttacaagcac
     4561 ctccacggct gtcatgtaat ccttccgctc cttaacaagg aaatcccgat tgtttgtgat
     4621 gaacacgcag atatggagaa gggaacgggt gttgttaaaa tcactcctgc acacgatcct
     4681 aacgactttg aagtcggtca gcgttgcaat cttccgattg tccgttgctt tacatatgac
     4741 ggtcacctta caggtgcaaa ggatgttgaa gaatacaacg aattaaaggc aaagggacag
     4801 cttgccgaaa acgaacccga ggttctcgac tgcggaaaat acgcaggcat gacaacaatg
     4861 gaggcaagag aggctattgt tgccgacctt aaagaaatcg gcgctcttaa agaggttgaa
     4921 gagcttactc acgatgtcgg cacttgctac cgttgtcaca caacaattga gcctatggtt
     4981 tcaaagcagt ggtttgtaaa aatggctccg cttgcaaagc ctgcaattga tgttgtccgc
     5041 aagggcgaca caaagttcat ccccgaaaga tttgaaaagg tttacttcca ttggatggag
     5101 aacatcaagg attggtgtat ttcccgtcag ctttggtggg gacacagaat ccctgcatgg
     5161 tactgcgatg actgcggcga agttatggtt gcaaaagacg cacccgaaaa gtgctgtaag
     5221 tgcggcagta cacacattca tcaggacgag gacactctcg acacatggtt cagttcggca
     5281 ctttggccgt tctcaacact cggctggccc gacaaaactc ccgaacttga gtatttctat
     5341 cccacaagca ctcttgtaac aggttatgat atcatcttct tctgggttgc aagaatgatt
     5401 ttctcagctt gtgaaaatat gggttctgct ccgttcaaga ccgtatttat gcacggtatt
     5461 gtaagagatg aaaacggcat caagatgtca aaatctctcg gcaacggtat tgatccgctt
     5521 gaagttattg acaaatacgg tgcagacgca ctccgtttct tccttgcaac aggcaacaca
     5581 cccggtaacg atatgcgtta ttccgacaag cgtgttgagg cttgcgctaa ctttgcaaac
     5641 aagctctgga acgcatcccg ttatgttcac atgaacattg acgaccacga tgttaagaat
     5701 gaacttccga aaactctcac aacagaggac aagtggattg tttcaactct caacacagtt
     5761 gcaaaagaag taaacgaaaa tcttgagaag tttgaactcg gcattgcagt tcagaagatt
     5821 tatgaattca tctgggactg ctactgcgac tggtacattg agcttgttaa gacaagactt
     5881 tcaagtgacg gtgaggacgc tcagaatgca agacaggtac ttctttatgt gcttgatcaa
     5941 attctcagac ttctccatcc gtttatgccg tacatcacag aagaaatctg gcagacaatt
     6001 cctcacgagg gcgaaacagt aatgctcgca aagtaccctg aatacaacgc agagcttgat
     6061 tatcccgagg catcggatga aatgaaaaaa attatggatg caatccgtgc aatccgtaac
     6121 cgccgtgcag agatgaatgt tccgccgtca agaaaagcaa aggtttatgt tgcaacaaag
     6181 tttgccgaca cattcagaga cggcacacag ttcattcaga agcttgcatc agcaagtgag
     6241 gttgaagttg cagaaagctt tgaaattgag ggtgctgtaa acattgtaac ggcagacgca
     6301 aagatttaca ttcctatgga cgagcttgtt gacagggaaa aggagcttgc aagactcaac
     6361 aaagagcttg aacaggttaa aaagcgtctt gcacagagcg agggcaagct taacaatcag
     6421 ggctttgttg caaaagctcc cgaggctgta attgaaaagg ttaagggaca ggccgcaaag
     6481 gaaagagagc agattgctct tatcgaagcc gcaattgccg cactcaaata attcggcaat
     6541 ccgataaaac agaataaaat tataatcaaa ggctgaaaga gcatagttgt tctttcagcc
     6601 tttttatgtt cgtgcagata gtcttaacag ggagagagta agctttttaa attgaaagtt
     6661 gaaaattgaa aatggaaaat tatagtcggg aagacagttt tcaaaataca aaacatcgtt
     6721 tcccaattgt tttggtttat ttggaattaa ctgttaaatt gtagcggcga acacggttcg
     6781 ccgctacaaa atatccgtaa ctatggtttg ctccatgaac aaaaataaca tccgaaacgg
     6841 tcaggaaaag tcaaaacgca gaattgtccg ctttaaaagt gaaatctgtg aaataaagaa
     6901 catttcttcc gaaacccaaa ttatcccttt cacacaatat gtagcaaaat ttgtacagaa
     6961 gcacgcctgt ttagcggtta taggtcggaa tatgggctga ttattgctat ttaactgttc
     7021 aatcttgata aaaataacag atatgtgaaa taaaaatatg tatatgtgct aattgcacaa
     7081 agaacggtgt tgttttttag gcattgctac aaatctgcga aaaacagaaa ataatgcttg
     7141 aaatccacat ttttgtgttg tattatatag gtgtcaaagg aaataaccga ttcggaaaac
     7201 ggaaatcaaa caaacgaatc ggaaacatta aatcttattt aataaagaaa aggagaaaat
     7261 attatgcatg ctttaaaaag atttggtaca gctttcggcg gatataaaat gatggaaaac
     7321 taccccgttc ccgaatgcgg tccggacgac attattcttg aaatcaaggc agccgctatc
     7381 tgcggtgccg acatgaaaca ctggggtgtt gacaacggtt acgatgacac aaacatcact
     7441 cccgatcagc agcagtcggt tcgtggtcac gagttcgcag gcgaaatcgt taaggtcggc
     7501 gaaaatgtaa aagactggca tatcggtcag agagttgttt cagacaacag cgcacatgtt
     7561 tgcggcgtat gtccggcttg tgagcagggc gacttcctct gttgcgaaca taaagtaaac
     7621 cttggacttg acaacaacac atggggcggc ggcttctcaa agtattgtaa agttccgggc
     7681 gaaatcctcg caattcacaa acacgcaatc tgggaaattc ctgagggcat caagtacgaa
     7741 gaagcttgtg tactcgatcc tatctgtaac gcatacaagg cagttgctca gcagtcacat
     7801 cttatccccg gacaggatgt tgtagtattc ggtacaggtc ctctcggact cttctcagta
     7861 caggttgcaa agcttatggg tgccgtaaac atcgtaatgg ttggtcttga cgatgatgta
     7921 aaagtaagat tcggcgttgc aaaagagctt ggcgctacac attgtgtaaa cggttctaag
     7981 gaagatgtag ttgcaagatg tacagaaatc tgcggcagag ataacctcgg actcgtaatt
     8041 gaatgttcag gtgctaacat agctctcaag caggcaatcg aaatgacaag accaaacgct
     8101 gaaatcgttc gtgtaggtat gggcttcaaa cctcttgaat tctcaatcaa cgacattaca
     8161 gcttggaaca agagcattat cggtcacatg gcttatgact caacatcatg gagaaactgc
     8221 atcagacttc tccagtcagg tcagattaag gttcagccga tgattactca tcgcctcggt
     8281 ctttcacagt ggcaggaagg tttcgacaag atggctaaga aagaggctat caaggttatc
     8341 ttccactacg attacgaaga ttaattaaag aaaatatgca tcagggagga ttttctaatg
     8401 agaaagctta tgattgcccc gtcagtgggt tgctgtgatt tgttccatgt tgaagaacag
     8461 gttaagctga ttaacgaaaa atcagattac ctccacatgg acattaaaga cggtgtttat
     8521 gttccgagtt acggaatcgg tcctgattat ctcgactacc ttaacaagca tgttgagaat
     8581 ctcaaaccaa tggatgctca ccttatggta aaacatcctc agcagtatct tgaaaccttt
     8641 gcaaaggcag gagctgctta cataacacct cacacagact gcatcgaagg cgacgcattt
     8701 gtaacgattc acaagatcaa agagcttggc tgtaaggcag gcgttgcatt aagtccctcg
     8761 gttccgcttt ctactattga atattacctc ccgttgcttg ataaggttac aatcatgatt
     8821 gttgatccgg gcatcgcagg tcagccggtt gatccgcaaa tgttcgtaaa aatcaaccaa
     8881 ctcgctaaga tcagaaagga aagaggtctt aacttcttaa ttgaagccga cggctcaatg
     8941 aacagcagtc tttaccgtcc gctttacgag gcaggtgcag atctcgtaat tctgggacct
     9001 ccggcattat ggaacaagga cacggatttc aataaggctt gggacataat ggaaagtgag
     9061 cttgagcgag agcttgacgg tgctgagaga aaaatctgat ttaaataaaa agtcaaagag
     9121 gaaattaaaa ggagaagaaa aatggctaac aaaacttctg tcaacattgg cgcaagactt
     9181 gaccgcttgc ctcaatcaaa atggcatgtt aagatctggc tggttacagc ttttgcactc
     9241 ttggtatgct ggtcaaacgg tatcggcggt tccgttcaga acattctttt gaacgaactt
     9301 cattggctcg aacccggttc aacacttctc gcaatgtggg gtacaactta cacagcagga
     9361 cagctcttcg gcgcactcat cggtggtcct atcggcgata aaatcggccg taaaaagagt
     9421 atccttcttt acgaaattat ccatatcatc gctatgatcg gcggcgctct ttcaccgaac
     9481 atcgcagttc tttatgtgtt cagactcctc cagggtcttg cactcggcgc tctccttgta
     9541 gttcttttcg caggcttcac agagtatgtt cccggtaaga accgtggtac atggtcaagc
     9601 cgtacatcat tcatcggcaa ctgggctcac ccaatctgca acggtattgc tcttcttatc
     9661 gtttcaacag gcgtaagcat gaatatgaac tggagaatcc agtttatgat tccttcaatc
     9721 ctttcaatca tcgcttgtat cttcatctat gttaaattcc ctgaatcacc gagatggctc
     9781 gaatcacagg gcagacttga tgaagctgat aaaattatga caagcattga gaaagaaatt
     9841 gaaaaatcaa caggcaaacc actcccaccc gttgaagtta caaatccaaa gcctgttaag
     9901 aagctcccat tcacagctct cttcaaaggc aaactcctca gaagaactat cgttggttct
     9961 ctcgtactca tcggtatgaa cacaatccag tacacactta tgaactggat gccgtcaatg
    10021 ctttcaggtc ttggctatga tacatcaaag tcacagttta tgacaatgtt cggactcttc
    10081 ggtgctccgt ttggtatctt tatcgcatct cttattatgg ataagattcc tcgccgtgta
    10141 atgggcgtta tcctccttgc aggcatggca ggtcttggtg ttgcaacagg tcttcttgct
    10201 ccgacactcg gaatgacagg actcattgtt atgacattcc ttcttaacac aatcatctat
    10261 atgtatgttt gctacgcatc agcagtttat gttcctgaga tgtggcctac atcggcaaaa
    10321 ctttcaggtt cgggcttcag taacgcaatg ggtcgtgtaa gtaacatctt cttcccattc
    10381 ctcgttacaa atgttgctgc aagcagcatg ggctcaggcg gtgtattcat tcttatcgca
    10441 attgttgcag caatcatcgc tgttgcagtt atcttcctcg gtgttgaaac cagaggcgaa
    10501 tccgttgaga acatcggtga tgtagagtaa ttctgcaaaa ccctctaata catacaacca
    10561 aaaattttag atgcggtgcc tgagtgcatc tggcaccgca aactaaaatc attagtaatg
    10621 gaaaacgaaa tacagcaatt ttaaaacatt caaaacttat ctttaaattt ttaacagcta
    10681 aaccatcaat tttttaatca caggcaaata aatttgaata tacgcagaag gcacatcggg
    10741 tgcaaatgct taaataaatt attataaaga aagtgcagga gattattatg aaaaattcag
    10801 aagctatctt agttactcca aagcagtttg aaattcagga atgtcccgtt cccgagccaa
    10861 aggatcacga gattcttatg aaggttgaat atgtcggaat gtgcggttcc gatatccacg
    10921 gctttgaatt cggtcctttc atcccaccta aggatcctaa ccagaaaatc ggtcttggtc
    10981 atgaggttgc aggcgaggtt gtaaaggttg gctcaaaggt tacaagattc aaagcaggcg
    11041 acaaggttct catcgaacca ggtgttccgg atgacaaatg cgaatactgc cgtacaggca
    11101 gatacaacat ctgtcctgat gttgacttta tggctacaca gcctaactac aagggcgcac
    11161 tcacacagta tatgacacat cccgaggagt ggacatacca cattcccgag ggtatgacaa
    11221 caatggaagc agctctcgtt gagccggcag cagtcggtat gcacgcagca atcctcggcg
    11281 gcgcacagct tggtaagagc attgttatcc tcggcggcgg cacaatcggt cttatggtac
    11341 ttcaggcttg taagagcctc ggtgcaacag acatcaccgt tgttgatatt atgcagaatc
    11401 gtcttgacct tgcattaaag cttggtgcaa gcagagttat caacggcaag gagcaggata
    11461 cggttgcact tctccgttca cccgaatatt tcggtgatca cggttgtgaa ctcgtattcg
    11521 aggcagcagg ttctgtattc acagctcagc aggctgttca gatcgttgca agaggcggta
    11581 agattatgat ggtcggcaca cagtcaaagc ctgttccgat tgacttcctc aagattaacc
    11641 gtgaggttat gattcaaaca tcattccgtt actgcaacaa cttcccgcag acaatcgagg
    11701 caatcgcaag cggtaagttc aatgttaagg atatggttac aaacatctat gattacaaag
    11761 atgttcagca ggcatttatg gacgctatcg accccgaaaa gaaagccaat atggttaagg
    11821 gcgttatcaa ggtagccgac tgatatacac agatttatta aaaattttca taaggaaagg
    11881 tgattaaaat gttatcagat atcagatatt gggaaaagaa ggcaacagag ggacactacg
    11941 ctattcctca cttcaatgta tggaatgcag aaatgctcat gggtgttatt gacgcagccg
    12001 aagagctccg tgctccaatc attatttcat tcggtacagg tttcacaggc aacacttctt
    12061 ttgaagactt ctgctacatg atggattcaa tggcaaagaa ggctactgtt cctgtaattg
    12121 agcattggga tcacggcaga aacatgacaa ttcttcagaa cgctgttaat cactgcatga
    12181 attcggtaat gcgtgacgca tcagctcttc cttatgaaga aaatgttgct gaaatcaaga
    12241 gatgcgttga ctacttccac gcttacaaca ttcctgttga ggctgagctt ggtcatgtag
    12301 gtaacgaaac agtatatgaa gaggcacttt cagaatacca gtacacagat ccttcaaagg
    12361 ctaaggaatt tgttgagaga acaggctgtg actcacttgc agttgcaatc ggtaatgttc
    12421 acggcgtata ttcttcagag ccaaagcttg ctttcgatgt actcgaggca gttgccaaag
    12481 aagtagatgt tccgctcgta ctccacggtg catcgggtat cggtgacgag gacattaaaa
    12541 aggctatctc actcggtatc gctaaaatca acattcacac agagctttgc caggctgcta
    12601 tggaagctat taatgctcac aaggacgagc cgttccttgc tgttgagcgt gaagtaagaa
    12661 aggctgttaa agagcgtgca atgtacaaga ttaagctctt tggcgatgac ggcagagcag
    12721 atgagtaata tgagtaatac agagaaaaaa cttgatgtta tctgtcttgg cgcagcggta
    12781 gttgacattc cacttcgtcc ggtatcaaga gatatttttg atatcgagtc atatccggtt
    12841 gacagaattg caatgtctgt cggcggtgac gcaatgaacg aggcaacaat catcagccgt
    12901 ttaggcttta aaacaggcat tatcgcttgt atcggtgatg acgctgcagg tcagtttatc
    12961 cttcgctcct gcgagaagga caacatagat gttgaaggca tcaagattga tccgacaatc
    13021 gacacctcaa tgaacattgg tcttgtaacc gatgacggtg aaagaacctt tgttacaaac
    13081 cgcaatggca gcttgtggaa agaaaacatt gagcatgttg actttgaaaa aatgaagcag
    13141 gcaaaaatcc tttctctcgc aagtatcttt aactgtcctc ttcttgacgg caagacgctt
    13201 gtaaagattt ttaaagaggc aaaggcggca ggtatgacaa ttaccgccga tatgataaag
    13261 ccaagactcg gtgaaactct tgacgatatc agagaagcat taagctatgt ggattacttc
    13321 ttcccgaact ttgacgaggc ttgccttatg acaggcgaaa ccgaagaatc aaaggttgcg
    13381 gacaagctct ttgaatgcgg cgttaagaat gtaatcatga agataggcaa gcgtggatgt
    13441 tacatccgca atgccgcagg agcaatgatt gttcctgcct gcaagggtgt aaccgcaatc
    13501 gacacaatcg gtgcaggcga caactttgca tcaggcttta tttcggcact tcttcagggc
    13561 aaggatatcc gtgactgcgg tatctatgct aactgcacag cggctatttc ggttcagtat
    13621 gtcggcgcaa caacaggcgt tcggaacaaa gaaatggttg aagaaatgct tgaaaagtat
    13681 aaaaaagatt atatcctgta atagtaataa atctgtgaca ttacgccctg tgttcgtaag
    13741 aatgcagggc aaccccccac caatatatta ttaaagaaag ggcagatgca aaatgaagac
    13801 gatgaagctt ggcaaaacag gcattgatat ttcaagaatg ggactcggca cctggtcaat
    13861 cggcggcggc tcggcatggg gcggcgacca cgaccttcag gttgttgtag atacaatccg
    13921 tgaagctccg aagctcggcg taaaccttat cgacacagct ccgggttaca acttcggcaa
    13981 cagcgagaaa atcctcggca aggctcttga gggcatgaac cgtgaagatg ttgtaatcat
    14041 tacaaagtgc ggtgttacat gggatcagga gatgaagggc gaccttttca acaaggtaaa
    14101 cggcattcag ctttataaga acctcaacag tgcttcaatc aagagagaaa ttgaagaatc
    14161 actcaagaga atgggcacag actatgttga tgtatatatg acacactggc agtcaatcga
    14221 gggcagcgag tattatgttc cgattcagaa gactatggag gttctcaacg atctcaaggc
    14281 tcagggcaaa atcaaggcta tcggtgctgc aaatgtagat atcaagcaga ttcaggagta
    14341 cctcaagtgg ggcgagcttg atatcgttca ggctaagtat tcaattcttg accgtggcat
    14401 tgaggatgaa atcattcctt gctgtcgtga aaacggtgtt acaattcagg cttactcacc
    14461 gctcgaaatg ggacttctct cgggtacatt cccaagagat tacaagcctg taggcgcaca
    14521 gattccaaag aaatggttcc agcccgaaaa tatgcagaag gctatggatg ttatggatca
    14581 gtggaagcct ctttgtgaaa aatataactg cacaattgct aaccttgctc ttgcatggat
    14641 tcttgctcag ggtgacttta tcaaccttct cagcggttct actactgtag atcagattgc
    14701 cgagaatgtt aagtcagccg aacttgaact tgattccgct gatgttgcaa tgatgcgtga
    14761 tatggttgag gctatcgaca aataattaag ggttgcttaa tacacctttg tgtatagggt
    14821 gaaggaatcc gctcccgatt gttaaaatta ttgtaaaaac agtcgaattc cctttacttt
    14881 tgttgataat tatgttagta tgtaataaaa gttgagggaa atgtttatgg caaagagaat
    14941 tgacgaaatc agacaaaagg ttgaaaccga aggcgaggtt ttcgtctcgg atttaagcag
    15001 aatatataat gtaaccgaag aaactatcag gcgtgatctt gaaaagctga agaatgacgg
    15061 tatcattacc agaactttcg gaggagctgt tcttaacaca actcctcaga atgacagagt
    15121 gcatttcttt aaacgcaagg gaatcaacct tgagagcaaa aagaaaattg ctaagctgct
    15181 tgttgataaa tttatcggtg tgaatacgat gatgattgat aacagcacaa ctgttcttga
    15241 ggctgttaag ctttttaagg acaatgaaaa tcttaccgca attacctttt ctgcacagat
    15301 ttttcaggaa ctcgacactt gtaaaatgaa gctggtttca accggcggac tttatgatga
    15361 aaaaacccgt tctttctacg gaaaacctgc aaaagaggct atgaaaaagt acaatgttga
    15421 ccttgttctc ctcggatgca aagggcttga tatggaaaga ggcataactg attcttccga
    15481 aggtgaggct gatttgaagt ctgagatggc aagccgtgcc gaatgtgtgg tattacttgc
    15541 agaccattca aaattcaacc atactgcgtt tgtcaatttt ttggattggg ataggatcga
    15601 ttatctgatt accgacgaaa agccgtcaca ggaatggctt gagttttgtg agcagatgaa
    15661 aataaaactc atgtactgat ttaccttgtc cggtacatag tttgagccag tgctttttat
    15721 aattttaccc attaaagctc ctgttctttt taatcaaaaa cggcagcagc ttttgcgctg
    15781 tcaaaacttt agcagattta acagttttga gaacgcaaag aaattatttt tattattatt
    15841 ggatagtatt gttaaatctg agttgaattt taaacatagt aattatttta tatatcacta
    15901 tggaaattct attaattacg gttatttttg cagacgggag accaatcgga gacggttccc
    15961 gtcacattaa ggacacaccg attaaatatg aggataggat gtgcaaatat tccttgagac
    16021 agtttattcg gcagttccgc aataaagggg ttacccaaat tcacagataa ggagatgtaa
    16081 aaatgacaaa acaaaaggag aagaagaaag cttcaattgt caagaaaatg atgattgccg
    16141 ttgtcgcagg ttttgtagtt ggctttatat gtctgctgat taaaagtgcg gtaatgggaa
    16201 cagacggcga aggagcctgg aaagttgttg aggcaatttt attccaggat atcaccgcaa
    16261 caaaagatat cgaaggactc ggcttgttct acatcgtagg tcagctgttt atgaagggac
    16321 ttcagatgat gattgttcct ctcgttcttt gttcgctgac acttgcactt tgcagccttg
    16381 caaacccaaa aaagctcggt cgaatcgcag gtaaaacatt tgtgtcctac ctttgcttct
    16441 atatcgtagc agcagctctt gcaggcgcag cagcttatct tgcaaagagc cttggttggt
    16501 tcacagtcaa tcttccggca caacaggctc aggacatcgt tacaatggag ggttacaacc
    16561 cgctcgttac aattgtaaac gctgttccga gcaatatgat taacgcaatg tcatctaaca
    16621 acacaattct ttgcgttgtt gttatcgcaa ttatctttgg cctttgtatg acaaagatgg
    16681 gcgaaaaagc agatccgctc aagaaagttt ttgaaaacat caacgatatc gttcagatgt
    16741 tcctcaactt ccttatcaac aagattgctc ctatcgcaat tttctgtatg atcgtaagag
    16801 cactcgccgt ttacggaatt gagtacattt caccgacaat gatgtggatt gttgtaacaa
    16861 ttgttgtaag ccttcttctt gtttgcacaa tttacccaat cggtattttc attacaacag
    16921 gacttaatcc gtttatcttc cttaaaaagg cggcaaaaat cggtatgttt gcagcagcaa
    16981 caaactcgtc tgccgcaacg cttccgctta ataaggaaac ctgtattaac gaactcggct
    17041 gttccgaaga aatcagcagc tttgttcttc caacaggtat gacaatcaat atgaacggta
    17101 caaccgcaat gcatatgatt gccattacat tcatcgccac agccgcaggc gtcaacatta
    17161 ctcctgcaac cctttgtctt acagcattcc tctcaatctg cacggcagtc ggtactccgg
    17221 caatccctgt tgcaggcaca acaatggttt atgttgtaat gatgggactt ggcttgaact
    17281 cagatctttg tatgattggc tactcgctca ttcttgccat gaactacctc ccgggtatgg
    17341 ctgttatcac acttaatgtt atcggtgacg ccgctaccaa tgttatagtt tgtgcaaaag
    17401 aacattgcct taacaaagaa atctataaca gcaaaacccc taaaaacaac tgatttcagt
    17461 aagataactc caggaaaccc cttgtaaaac cgaaccgccc tcgacagcgc atgcactgcc
    17521 gagggcggct tagttttacg gtgagccacc gttcccagtt cgtgaagata gtatggtata
    17581 aaaaaggaca tattatgccc gagcgaaagg tttaattaaa tgtaaaattg aaaattgaaa
    17641 atggaaaatt gaaaatggaa aatt
//
LOCUS       DAOMVE010000019        17499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_629_length_17499_cov_3.309161, whole genome shotgun sequence.
ACCESSION   DAOMVE010000019 DAOMVE010000000
VERSION     DAOMVE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 17499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17499
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_17499_cov_3.309161"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="PLS64_02110"
     CDS             complement(<1..1460)
                     /locus_tag="PLS64_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HRL42661.1"
                     /translation="MKKIAVGILAHVDSGKTTLSEALMYSSGNITKLGRVDHRDSFLD
                     TFSLERDRGITIFSKQAVMKYNNTEFTLLDTPGHVDFSAEAERTLQVLDYAILVISGT
                     DGVQSHTCTLWKLLKKYNVPCFIFVNKMDLDGADKLSVMAQLRTGLDENCVDFTYPNS
                     DGFRESVAVCDEKILEKYYETDAVEKTDITGAIKERKIFPCMFGSALKLDGVKAFLNL
                     LDEYTVMPSYGAEFGAKVYKIAEDNQGNRLTFMKITGGSLKVREILQSEKNTNAEKVN
                     RIRIYSGEKFTAVEEAVAGTVCAVTGITFTSSGDGLGVEKNSGVPVLEPVLTYKVELE
                     DGTDAHTALTKLKTLENEDPQLNVVWNSRLGEIHIRLMGEIQLEVLRCIIKERFGMNV
                     SFSTGSIIYKETIENTVEGVGHFEPLRHYAEVHLLLKPAKRGSGITINSRCKEDLLDR
                     NWQRLILTHLCEKTHLGVLTGSPITDIEITLVSGKA"
     gene            complement(1495..1971)
                     /locus_tag="PLS64_02115"
     CDS             complement(1495..1971)
                     /locus_tag="PLS64_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL42662.1"
                     /translation="MCEVTIRRAKFKDIPQLDKLLFEVHKIHSDIRPDLFTVGEKKYT
                     DEELERIIVDEQKPIFVAEHNGKVKGYAFCIFKQNLESKSVTNIKTLYIDDLCVDEDT
                     RGMHIGTKLYNHVIDFARKSGCYNVTLNVWAGNDGAMKFYERIGFKVQKIGMETIL"
     gene            2365..2907
                     /locus_tag="PLS64_02120"
     CDS             2365..2907
                     /locus_tag="PLS64_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HRL42663.1"
                     /translation="MYEEIYAQAKQACNELCDVAKLKEGSIVVIGCSSSEVAGGTIGH
                     NSSLETAQAVFKGIYEVLCERKIYLAAQCCEHLNRAIIVEREAVPNAEIVNVVPQPKA
                     GGSFATTAYKTFKDPVALEEIKADAGLDIGGTLIGMHLKRVAVPVRLGIDHIGQAILL
                     AARTRPKFIGGCRAVYDDSF"
     gene            3089..5803
                     /locus_tag="PLS64_02125"
     CDS             3089..5803
                     /locus_tag="PLS64_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRL42664.1"
                     /translation="MSGIIVSVIVPFLESNNKLIDTLSSISEQKNINQENIEVLIVDA
                     TDKTSGKIASGSMSMCKVIYAKSVKSFADACNLAASKATGKYITVCNCGDTFSDNYFE
                     SCIKVFNKKEKIPFVAGHRFCVNPVFREKKEFRLNKGQIESSVVSLFDNPNLINLELT
                     GTFYRTEIFKSYKMNNKLKYESADDFALRIQLDYPEYVYLDEIEFDYFMPVSDDFMYY
                     VPTNYKDWYTDSLNNFLKPLINDSKDRDGNIPLFIQFYIVFNITTKFLANMNNRNKRN
                     MNDEELAVFFETARECFKFANDGFVLNKDKYVSLGYSEEAAEMFYMIKHNCVFKDMPF
                     EYSEGKKEVYLNCNNVYISKLTDQRVGMHVMDYRDGKLVIDGSFRQVFDLEDFELYVQ
                     FGDKRQVLTNTDRYSLTKYFGISAYKKFTFHLELELDPEKPQQTVAFFARYKDSIIPL
                     KLSFLHHWSKFTIKPKNSYWRFNKYVAYIDNSSTIVICHASAMDTFKRELKFLPYVFM
                     ESKRSFITRIQYWLTRPFFKNKKIWLMYDKLYKGGDSCEYLYRYCADKKDGISRYYII
                     DKNTSDYKRLKADGLKPVKNRSFKHKMLFLNTDIALITNSNVFPFNGYSMDRSRFIRG
                     LCNFPSMCLQHGLSVQKCAMAQQRIVDNTQMYFLASKYEYKNLSNHVYNYQDFDILKM
                     TGIGRYDGLINNDKKQILLSPTWRMYNAMPVTTSEGEQRAYNPEFKHTTYYKIYNDLI
                     NNKKLIDTAKRTGYKIKYVLHPILSSQVNDFIPDPYVEVVSSVGDFNYETAFQESSLM
                     VTDYSGVQFDFAYMKKPLVYFHPSQLPAHYDDGGFFYDTMGFGEICTESDQLVDLLCE
                     YMENGCKMKPKYVERVEDFYQFDDHNNCERIYNEIIKYQQQVNKDKLK"
     gene            6105..7208
                     /locus_tag="PLS64_02130"
     CDS             6105..7208
                     /locus_tag="PLS64_02130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRL42665.1"
                     /translation="MSKIKRQSGIELLRIIAMIQIIFLHAYQYGLLHDASKAAGDIDG
                     ILVTFVWSLCRTPVDVFIMISGYFMITSQFDIKKTIRRGGKIYGAMIFYSIILSIIFF
                     ISDPSLININSVIKAFTPLMSRTWYFLSNYLIILLLSPFLNKMLASLSKKHYLYFMGI
                     VFVAVSLWSTLAEIHGINNVISVNKILDPYMGKSLGGFLLMYIIGGYLRLFVKQKPLE
                     KRKPNFRYLGIFVGLCVLDFVLASVFPQYKPAFGMFNNPIVLAESAMLVLFFRDFNFS
                     SKFVNTVAGTTLGIYAIHENPYVRDCLWNVANFSNKHLYDTLIYIPIVIITILLIFAG
                     CCVIELLRLKLFEFVGNKINARKSITSGNRCTK"
     gene            complement(7257..7814)
                     /locus_tag="PLS64_02135"
     CDS             complement(7257..7814)
                     /locus_tag="PLS64_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL42666.1"
                     /translation="MICFKKVTKQICDYKSVKELYLSAFPAVERVPFWLLMKKSKNEG
                     ADFYAIYDEEKWIGLIYAITDGEVVYVYYYAISEKERGHGIGVAVLDEFKNLYKGKRI
                     FLAIEQLEEENAPNQKQRMSRYKFYQRCGFLRLNAKIVEAKITFDVMAYGGEVNPESY
                     NKMMKKYLGKIMSHFIKTEMKNFKI"
     gene            complement(7867..9231)
                     /locus_tag="PLS64_02140"
     CDS             complement(7867..9231)
                     /locus_tag="PLS64_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL42667.1"
                     /translation="MNGSKSVRNLTQGKISSQILFFAIPVIIGNLLQELYNVVDTLIV
                     GQTLGEIKLAAVGSTSSLNFFALGFFIGLSAGCSVITSQHFGANDMERVKKSVAAHII
                     IGVVSAVVLTVSFVLLVNPLLTMLGTTSDTFEYASRYLTIIYLGIPATMLYNLTASLL
                     RSVGDSKTPLYLLLFSSVMNVGLDLLFIIVFRWDVSGAAIATVISQLVSAVLCCVYIF
                     FKVKMLLPNRNSFKNLTSTVRDELKVGFPMGLQNSVISIGMMVLQYFVNQFGSYAVAA
                     YTIGNRVQLLIQNPMNSMSTVIATFAGQNEGAGRYDRIKKGVNFCLLICIAYSIVIGA
                     VSMIFAQPITGIFTSGSSQQTIDYSVQFIFWNCPFEWSLAMLFIYRGTIQGLKNGIVP
                     MIGSLIEVVMRIMTPVIFSSVIGYASICVAGPAAWTGSMLLMIAVYYVKIRKLSKTKT
                     VVAN"
     gene            10166..10420
                     /locus_tag="PLS64_02145"
     CDS             10166..10420
                     /locus_tag="PLS64_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007440242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HRL42668.1"
                     /translation="MSLNGTLKALSDPIRRNILTMLKGKKMIAGDIAEELGISPASLS
                     YHLNLLKKNDLVIESKEKNYVYYELNTTLFDELIMWLKQF"
     gene            10432..11100
                     /locus_tag="PLS64_02150"
     CDS             10432..11100
                     /locus_tag="PLS64_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HRL42669.1"
                     /translation="MKKLNKIMLALSILPTLLTSVFLFFMNDTVPMHYNASGAVDRYG
                     SKYENFIFPAIILFMYAFYMVYTKIYTKYSTNSVEKINNNVKITAILVTSIIAAFNIL
                     QLMFLILAVLKPDSTTFTDNVPFIINTVMGLMLIIAGNFLPKTKRNSFIGVKTSWTQS
                     SDLAWYESNRAGGIAFVVTGVLTIIESSFIKGINSTFIMVAILLISIAVAYIYAYVKV
                     KKSA"
     gene            complement(11180..13306)
                     /locus_tag="PLS64_02155"
     CDS             complement(11180..13306)
                     /locus_tag="PLS64_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRL42670.1"
                     /translation="MKFKIVYDKPQRIRFRCGAYAFDKEYEGAIYNLVTASPYVNSAQ
                     VSSANGGILVNYTKGSRSKIIDLVRAINVKTLKKNEPSAEFGIQKIDSDFHDNLFTLV
                     AKHYLSKMFLPAPIRTAITLYRSAKYIKKALKTLWNGKLTVDVLDGASVVACLCQRKF
                     KTAATVMFMLRISGLLEEYTHARTKAVLTDSLAIKTDRVWLVTDDGDVLIPIEDLRVG
                     DKIRVQTGKVIPVDGVVADGEATVNESSMTGEPLPVLKREGISVYAGTVIEEGSIVVT
                     VRQLASNTKISKIIELIGNSEELKAGVQSRAEALADRIVPFSFIGFFATLIFTKNITR
                     AVSLLMVDYSCAIKLSTPIAVISAVKEAADNDITIKGGKYLEAFANADTIVFDKTGTL
                     TNAEPVLEKVVAFGDYTEDEVLRISACLEEHFPHSVANAVVIGAEKRGISHSEEHTEV
                     EYVVAHGIATTLHGKRAIIGSKHFVVEDENVTVTKEQQNIIDEKSGSCSVLYLAIGGE
                     LSGALCISDPPRKEAKQAIDMLKKQGIKNVVMLTGDSYRAAKATAAMLGITDYKCQVL
                     PEDKHRYVEEMKQNGQKVIMVGDGINDTPALAAANVSVAMNDASDIARETADITIKGS
                     DLRALVRVRKLSKDLMKRINKNYRFIIAFNSALLLSGFMGVIQPSVSAFLHNASTMMI
                     CAKSMTPLTKKNDKGKALSLPEKAEQ"
     gene            complement(13306..13563)
                     /locus_tag="PLS64_02160"
     CDS             complement(13306..13563)
                     /locus_tag="PLS64_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1490 domain-containing protein"
                     /protein_id="HRL42671.1"
                     /translation="MLKVFKNEKLWCAVAGAAAVIVGKKILKADKTRSLAVTGLAKGM
                     KLQKDAKAAFQNMKDEASDICYDAKTEAGIHEDAAAEEVTE"
     gene            complement(13706..14450)
                     /locus_tag="PLS64_02165"
                     /pseudo
     CDS             complement(13706..14450)
                     /locus_tag="PLS64_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009244412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
     gene            complement(14562..>17499)
                     /locus_tag="PLS64_02170"
     CDS             complement(14562..>17499)
                     /locus_tag="PLS64_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRL42672.1"
                     /translation="KPLTRTYVELDFEHRGKKYRIIRTTSNEVAKKRGTGSKIEGASA
                     ELTGDDITTPLTKTGEVNNKISEIIGLKCDEYRQIAMLAQGEFKKFIESGSEKRSEIF
                     RKIFHTDIYKEFQDKLKTESAEKLEEKKKVCELIKSKTVAVEIPDDDSFAEISEKISL
                     YTDRTDLTVIDIEQFLLHLSVLSEMQKKDNAEIEKKRKELEKTILEEEKKKALAVEIN
                     NKFDTLDAEKLRLEQLLQSKPHYDEMLKKAENDEKILHSVKPKYDSFAESRKRYDSAL
                     KNCKNAEINRKSAEENLKLKQNVFAEKKSLEGKIATLNEESGKLRAKEDTYRKYFNLT
                     ANLSVYESVLQNSEKEISSNRKSYSDTENLKTKAEKFISENSDTEQKIFDAKSKITAL
                     KDKSGKAERLFAEYENLEKINAKLTNVKAECADLAKSATALNNEYNTKHNIYIMNMAG
                     VLADTLEDEKPCPVCGSLHHPNPAKHSENAPDKDTLDALKARCEVAENAVHKKSNEVT
                     RLETESESAKTNVTEFANALEVDAETLSAEMISQLLTEQKKLLKALENEASDLEKVRE
                     QREVCKAEISRFDEKLKKLDLVHTELIRKNADAKSKYNSAKEETESLKAEIKYSSVEE
                     LLNAAKACADKAEQIKSEIDLAQSELSKAKSDFDSKVSVENETAKQLKSFESELENKK
                     SELDIVLSENNILECPDFNLLTEDSIKELKREAETFTDSLKKSRATVEACEKSVEGKQ
                     REDVLKINELIDSYTSEKEKWDNLYTAGDRSLHNNQNIYNSVEKLKNDFEEKLRIADV
                     YENLKKTANGEIVSDNSKITFERYIMGSYFEQVLYYANLRFSKMTGGRYEIVRGESRD
                     KRISSGLEISVRDNFNNKERDISSLSGGESFQASLALALGLSDIIQQRNGGIRLDSIF
                     IDEGFGSLDNDSLASAIETLNDLTGNGNRLVGIISHISELKNSIGNKIEVVGTKSGSS
                     IKIITD"
BASE COUNT         5019 a         3709 c         3301 g         5470 t
ORIGIN      
        1 tgagcttttc ccgaaacaag cgtgatttca atatctgtaa tcggcgagcc tgtcagcaca
       61 ccgagatgtg ttttttcaca gaggtgagta agaatcagcc tttgccagtt gcggtcgagc
      121 aagtcctctt tgcaacggga attaattgta attccgctgc cacgctttgc aggtttcaaa
      181 aggagatgaa cctctgcata gtgtcttaac ggctcaaaat gaccgacacc ctcaaccgta
      241 ttttcgatag tttctttata gattatactg cctgtactga acgatacatt cataccgaaa
      301 cgctctttta ttatgcacct cagcacctca agctgaattt cacccataag tcggatgtgg
      361 atttcaccga gtctgctgtt ccacacaaca tttagctgag gatcttcgtt ttcaagcgtt
      421 ttcagctttg tgagagccgt gtgggcgtct gtcccatcct ctaattcaac cttgtaagtc
      481 agaacaggtt cgagtacggg aactccggaa tttttctcaa cacccaagcc gtcacccgat
      541 gaagtgaatg ttattcccgt taccgcacaa acggttcctg caacagcctc ctcaacggct
      601 gtgaatttct cgcctgaata aattcttatt ctgtttacct tttcggcatt ggtatttttt
      661 tcgctttgga gaatttcacg cactttaaga cttccgcccg taattttcat aaatgtaagg
      721 cggttgccct gattgtcctc tgcaatttta tagacctttg caccgaactc agcaccgtaa
      781 gagggcataa ccgtgtattc gtcaagcaga ttcaaaaatg cctttacacc gtcaagtttg
      841 agtgccgaac cgaacataca cggaaagatt ttgcgttcct taatcgcacc tgttatgtcg
      901 gtcttttcaa cggcatctgt ttcataatat ttttcaagaa ttttttcatc acatacggca
      961 acactttcac gaaagccgtc ggagttcgga taggtaaaat caacgcagtt ttcgtcaagc
     1021 cctgtacgca gctgtgccat aacggaaagc ttgtccgcac cgtcaagatc cattttgttg
     1081 acgaagataa aacacggcac attgtacttt ttgagcagtt tccaaagtgt gcaggtgtga
     1141 ctctgaacac cgtcagttcc gctgattaca agaattgcgt agtcaagcac ctgaagtgta
     1201 cgctctgctt ctgccgaaaa atcaacatga ccgggtgtgt caagcagagt aaattccgtg
     1261 ttgttgtact tcataaccgc ctgttttgaa aaaattgtaa ttccacggtc acgctcaagc
     1321 gaaaaagtgt cgagaaacga atccctgtgg tcaacccgac caagctttgt tatgttgccc
     1381 gaggagtaca tcagcgcctc tgaaagcgta gtctttcccg aatcgacatg agccagaata
     1441 ccgactgcaa tttttttcat cttatcacca tacccgaaaa tttatatatt tcttttagag
     1501 aattgtttcc ataccgattt tttgaacctt aaaaccgata cgctcgtaaa atttcattgc
     1561 accgtcattg cctgcccaca catttagcgt aacattataa caaccgcttt ttcgggcaaa
     1621 atcaataaca tggttataca gctttgttcc tatatgcatc cctcttgtat cctcgtcaac
     1681 acacaaatca tcaatataca gggtttttat atttgtgact gacttgcttt caagattttg
     1741 cttgaaaata caaaaagcat atccttttac ttttccgtta tgttctgcaa caaaaatcgg
     1801 cttttgctca tcaacaatta ttcgctcaag ctcctcgtct gtgtatttct tctcaccgac
     1861 tgtaaaaagg tcggggcgga tatcgctgtg tatcttgtga acctcaaaaa ggagcttgtc
     1921 aagctgcggt atgtctttaa attttgcccg tcttattgtt acttcacaca taatttcatc
     1981 ttccaatcat ataaaaaata ccttttgcaa tttcgttttg ccgaaacctt caacatttat
     2041 tttaccacat taaaaaatta atgtaaacaa agcaaaaagg cttgcgacca aaattgaccg
     2101 caaacctttt cgggagtaaa tatgaacgaa agtaattatc agttttatgt tgttatataa
     2161 gatttttgta aagctcttaa atttcttcaa cacttgcaga ataaacctac ttgtctcatt
     2221 atacacctat atatctaaaa tttcaagaac cattttaaca tttctatgtc ttttgctgtc
     2281 atttgcatta aaaacttgta ctttgcaaaa agaatggtat aatataatca gaataaaatt
     2341 tacataacca aggagttgac atatatgtat gaagaaattt atgctcaggc aaaacaggct
     2401 tgtaatgagc tttgcgatgt tgccaagctt aaagagggca gtattgttgt aatcggctgt
     2461 tcttcaagcg aggttgcagg cggaactatc ggacacaatt caagtcttga aaccgctcag
     2521 gctgttttca agggtatata cgaagtgctt tgcgaaagaa aaatttacct tgccgcacaa
     2581 tgctgtgaac acctcaatcg tgcaatcatc gttgagcgag aggctgttcc gaacgctgaa
     2641 atcgtgaatg ttgttccaca gccaaaggca ggcggttcgt ttgccacaac agcatacaaa
     2701 acattcaaag atcccgttgc gcttgaagaa atcaaggctg acgcaggtct tgacatcggc
     2761 ggaacactta tcggcatgca ccttaaaaga gttgccgtac ccgtaagact cggcattgac
     2821 catatcggac aggcaattct gcttgccgca agaacccgtc ctaaattcat cggcggttgc
     2881 cgtgccgttt atgacgacag cttttaattt tattacaatt gacgggcggc agttttcgga
     2941 ctgtcgcctg ttttttatta ctgcttatta aaatttcaac atattacctt gttttccgca
     3001 aatttgcgac atacaatatc aaagaaaagt ttgcattttt cactaactgt ggtataatct
     3061 aatgagataa tttttttctg gaggtactat gagcggtata attgtttctg ttattgtccc
     3121 ctttcttgag agcaataaca aattaataga tactctgtca tcaatatccg agcaaaagaa
     3181 tattaatcaa gagaacattg aagtgcttat tgttgacgca acagacaaaa cttcaggcaa
     3241 aattgcaagt ggcagtatgt caatgtgcaa ggtgatttat gcaaagtcgg ttaaaagctt
     3301 tgccgatgcc tgcaaccttg ctgcgtcaaa agcaacaggc aaatatatca cggtgtgcaa
     3361 ttgcggcgac actttcagcg ataactattt tgaaagctgc attaaggtgt tcaacaaaaa
     3421 ggaaaaaatt ccttttgttg ccggtcacag attttgcgta aaccctgttt tcagagagaa
     3481 aaaggaattt agacttaaca aaggtcagat tgaatcaagc gttgtcagcc tttttgataa
     3541 tcccaacctc atcaatcttg agcttacggg tactttttac agaactgaaa ttttcaaatc
     3601 atacaaaatg aacaacaagc tcaagtacga aagcgcagac gattttgcac tcagaattca
     3661 gcttgattat ccggagtatg tatatcttga tgagattgaa tttgactact ttatgcctgt
     3721 aagcgatgac tttatgtact atgttcccac aaactacaag gactggtaca cagattcgct
     3781 taacaacttc ttaaagcctc tcatcaatga cagcaaggac agggacggaa atattccgct
     3841 gtttattcag ttctatattg ttttcaatat tacaacaaag ttccttgcaa atatgaacaa
     3901 ccgcaacaag cgtaatatga acgatgaaga gcttgcggtt ttctttgaaa cagcaagaga
     3961 atgttttaag tttgcaaatg acggttttgt tcttaacaaa gacaagtatg tttctctcgg
     4021 ctacagtgag gaagccgccg agatgttcta tatgataaag cacaattgtg ttttcaagga
     4081 tatgcctttt gaatattccg agggcaaaaa agaggtttac ctcaactgca acaatgtcta
     4141 tatttctaag cttacagacc agagagtcgg tatgcatgtt atggactacc gtgacggcaa
     4201 gcttgttatt gacggctcgt tcagacaggt ttttgacctt gaagattttg agctttatgt
     4261 tcagtttggc gacaaaagac aggttcttac aaacacagac cgttattctc tgacaaaata
     4321 tttcggcatc agtgcgtaca agaaattcac cttccacctt gagcttgagc ttgatcccga
     4381 aaagcctcag cagacggttg cattctttgc aagatacaag gattcaatta ttccgctcaa
     4441 gctttcattc ctgcaccatt ggtctaagtt tacaatcaag ccaaagaact cctactggag
     4501 attcaacaag tatgttgcct atattgacaa ctcaagcaca attgtaatct gccacgcatc
     4561 tgctatggat acattcaaga gagaactcaa gtttctccca tatgttttca tggagagtaa
     4621 acgcagcttc attacaagaa ttcagtattg gcttacaaga cctttcttca agaacaagaa
     4681 aatctggctc atgtacgata agctgtacaa gggcggtgac agctgtgaat atctttacag
     4741 atattgtgca gacaaaaagg acggcatttc aagatactat atcattgaca aaaacacttc
     4801 cgattacaag cgactaaagg ctgacggact caagcctgtc aagaaccgtt cattcaagca
     4861 caaaatgctg tttctcaata ccgatattgc gctcattaca aactcaaatg tattcccgtt
     4921 caacggctac agtatggatc gttcaagatt catcagaggt ttgtgcaact tcccttcaat
     4981 gtgcttacag cacggacttt cggttcagaa gtgtgcaatg gctcagcaga gaattgttga
     5041 caacacacag atgtacttcc ttgcgtcaaa gtatgagtac aaaaacctga gcaaccatgt
     5101 ttacaactat caggattttg acattctcaa gatgacaggc atcggcagat atgacggact
     5161 tatcaataac gataaaaagc agattctgct ttcacccacc tggcgtatgt acaatgcaat
     5221 gcctgttaca acaagcgagg gtgaacagag agcttacaat cccgaattca agcacacaac
     5281 ctattataaa atctacaacg accttatcaa taataaaaag ttgattgata cggcaaagcg
     5341 tacaggctac aagattaagt atgttcttca tccgatttta agctcacagg ttaacgactt
     5401 tatccccgat ccgtatgttg aggttgtttc atcggttggc gatttcaact acgaaacagc
     5461 ttttcaggag tcaagcctta tggtaacaga ctattcgggc gttcagtttg actttgctta
     5521 tatgaagaaa ccgcttgtat attttcatcc gtcacagctt cctgcgcact atgatgacgg
     5581 cggattcttc tatgatacaa tgggattcgg agaaatttgt accgaaagcg accagcttgt
     5641 tgacctgctc tgcgagtata tggaaaacgg ctgtaaaatg aagccaaagt atgttgaaag
     5701 agttgaagat ttctatcagt ttgacgatca caacaactgt gaaagaattt acaacgaaat
     5761 catcaagtat cagcaacagg ttaacaaaga caaattaaaa taatatttaa gcggtcagtt
     5821 tttgctgacc gtttttgttt tgccaatact tctcaagctc aatatacttt tattgctttt
     5881 tgtacatatg tacaaatttt gtatatattg tttaaaaatt acttgttttt ctcttgaaat
     5941 actacaccgc ataaattgtt ataacaggca aattatggta taattggtac ttgtagcaat
     6001 gttgcaaatt ttacgaaaat tgttaagaaa acgaagttcc tgtcagaaat tgacagacac
     6061 aaatccccta ctttttggga tttccgttta cggaggattt aaaaatgagc aaaatcaaac
     6121 gacagtccgg tattgagtta ctgcgaatta ttgcaatgat acagataatt tttttgcatg
     6181 cgtatcagta cggattactt catgacgcat caaaggccgc aggcgatatt gacggtatat
     6241 tggtgacatt tgtatggtca ttatgcagaa cccctgttga tgtgtttatt atgataagcg
     6301 gctacttcat gataacctcg cagtttgata taaaaaagac catacggcgt ggcggaaaaa
     6361 tctacggtgc aatgattttt tactcgatta tactttcgat aattttcttc ataagcgatc
     6421 cgtcgctcat aaatataaat tcagttataa aggcgttcac tccgctaatg tcacgaactt
     6481 ggtattttct gagcaattat ttgattattc ttctgctcag cccatttctc aacaaaatgc
     6541 ttgcgtcact ttctaaaaaa cattacctct actttatggg aattgttttt gtggcagtaa
     6601 gtctatggtc aacgcttgcg gaaattcacg gaataaacaa tgtgatcagc gtcaacaaaa
     6661 tacttgatcc ttatatgggc aaaagtcttg gcggattcct tttgatgtac ataatcggcg
     6721 gttatctgag gttgtttgta aagcagaaac cgcttgagaa aagaaaaccg aatttccgct
     6781 atctcggcat tttcgtcggc ctttgcgtat tagattttgt attggcatct gtttttccac
     6841 aatacaagcc ggcattcggt atgttcaaca acccgattgt cctcgccgaa tcggcgatgt
     6901 tggttctctt tttcagagat ttcaatttca gctcaaaatt tgtcaataca gtagccggca
     6961 caacgctcgg tatatatgca atccacgaaa atccgtatgt tcgtgattgt ctgtggaatg
     7021 ttgcaaactt cagcaataag cacctctatg acaccctgat ttacattccg attgtaatta
     7081 taacaatcct cctcatattc gcaggctgtt gcgtaattga gcttttaaga cttaaactct
     7141 tcgaatttgt cggcaataaa atcaacgccc gcaaatccat aacatccggc aaccgctgta
     7201 caaaataata tatcttataa aaaacaacac cgacagatta agccaaaagc taacctttaa
     7261 attttaaaat ttttcatttc tgttttgatg aagtgcgaca taattttgcc gagatatttt
     7321 ttcatcattt tgttatagct ttcggggttg acttcaccgc cgtatgccat aacatcaaat
     7381 gttattttcg cctcaacaat ctttgcattc agccttaaaa atccgcaacg ctgatagaat
     7441 ttataacgac tcattcgctg tttttgattg ggggcatttt cttcttcaag ctgttcaatt
     7501 gcaagaaaaa ttcttttgcc cttatacaaa tttttaaatt catcaagcac ggcaactccg
     7561 attccgtgtc ccctctcctt ttcggaaatt gcatagtagt acacatagac aacttcgccg
     7621 tccgtgattg catagataag tccaatccac ttttcttcat cataaattgc gtagaaatct
     7681 gcaccctcat tctttgattt tttcatcaaa agccaaaacg gtactctctc aacggcagga
     7741 aatgcggaaa ggtataattc ttttacagat ttataatcac aaatttgttt tgtgactttt
     7801 ttaaagcaaa tcataaaaat ccctttcgtt attataatca cagggaatat tgtaccacat
     7861 aaaggattaa tttgccacaa cagttttcgt ctttgaaagt tttcttattt taacataata
     7921 aaccgcaatc ataaggagca tacttcctgt ccatgcggca ggaccggcaa cacagattga
     7981 tgcgtaacct attacagaac tgaaaatgac gggcgtcata attcgcataa caacttcgat
     8041 aagcgaacca atcataggca caatgccgtt tttaagtccc tgaattgttc cacggtatat
     8101 aaacagcatt gcaagcgacc actcaaacgg gcagttccag aagataaact gaacggaata
     8161 atcaatagtc tgctgtgagc ttccgcttgt gaagattcct gttatcggct gagcaaaaat
     8221 cattgaaacc gcaccgatta caattgaata tgcaatgcaa attaacaggc agaaattgac
     8281 accttttttg attctgtcat atcttcctgc gccctcgttc tgacctgcaa atgttgcgat
     8341 aaccgtactc attgagttca tcggattctg gatgaggagc tgaacacggt tgccgattgt
     8401 gtaggcggca acggcatatg aaccgaactg atttacgaaa tactgcaaaa ccatcattcc
     8461 gattgaaatt actgagtttt gaagtcccat cgggaaaccg acttttagct cgtcccgtac
     8521 agttgaagtc aaatttttaa agctgtttct gttcggcaaa agcattttca ccttgaaaaa
     8581 aatgtacaca caacagagaa ctgccgacac aagctgagag attaccgttg caattgccgc
     8641 acccgaaaca tcccacctga acactatgat aaataacagg tcaagtccca cattcattac
     8701 ggatgaaaag agcaacagat aaagcggagt tttgctgtca ccaaccgaac gcagaagtga
     8761 tgccgtaagg ttgtagagca ttgtggcggg gattccgagg taaattattg taagatatct
     8821 gcttgcatac tcgaatgtat ccgaggttgt tccgagcatt gtaagcagcg gattaacaag
     8881 aagtacaaac gaaacagtaa gcacaacagc cgacacaacg ccaattatta tgtgggcggc
     8941 aacgctcttt ttcacccgtt ccatatcgtt tgcaccgaaa tgctgtgaag tgattaccga
     9001 acagccggcg gaaagtccta tgaaaaatcc gagtgcaaaa aagttgagcg aactcgttga
     9061 accgacagcc gcaagcttga tttcaccgag agtctgaccg acaatcagcg tatcgacaac
     9121 attgtaaagc tcctgtaaaa ggttgccgat tattacggga attgcaaaaa ataaaatctg
     9181 acttgaaatt tttccctgag tcagatttct aacagatttt gaaccattca taacattcac
     9241 acttcttatc aaaaattttt atgtttttaa tatctttttc gtttaaattt aagtcttggc
     9301 aacactctga ttttgattgt tcaaatgcag agtatgttgt atatagcaaa cagattggaa
     9361 tctgtgcaaa ggagcgatag ttcctcggga tttcagtcat tagactgaaa tcctaagaat
     9421 taccgcactg ccaagcggta cggtacttct gcactagatt atagcacagc gatttgtgaa
     9481 tgtaaatgta taggaatgac gaaataaaga cgcttttgtt ttgaaaaaac tttacacaat
     9541 gcacagtatt gattttgtcc tattttatta cagcataatt ttataaccaa ataataagta
     9601 tttcacatta tccgattatt attgactgtt ttaagaacac aaccgttttg atgaaaattt
     9661 gcaaattgtc aatattttgt tattctaagg tttcagcaaa aacaaaacag agccgtaaaa
     9721 acgactctgc cttgtaacat tagagaaaat atttatgaaa acaaactgtc ttattaatca
     9781 gtctttttta acattgaaag cgtccgtacc gactgctgta tcgttaatta ctgtattaat
     9841 atttcaactg attttgaaac accctgtcgg gaggtaagca catcctcccg agtaaaggct
     9901 ttcggtaccc gcaagggaag tccacatgat ttattcaaaa gagttagcct ctgctaactg
     9961 agtattatta tataccactc aacaaagaaa atcaagagaa attttttatt tttttctgca
    10021 atattcgtca gagatatttt aaagctattt aaaaatattt ttaaatagtt cttgactttt
    10081 cagtaaatca ggctataata aagctacaag ctatttaaaa tattttttaa atagatgtga
    10141 taggactttg tgaggaggta tctgcttgtc actgaacggt acacttaaag cactttcaga
    10201 tccgataaga agaaatattc tgacaatgct gaaaggcaag aaaatgattg caggcgacat
    10261 tgccgaagaa cttggaattt cacctgcatc gctttcatat cacctcaatc tgttgaagaa
    10321 aaacgacctg gtgattgaat caaaagaaaa aaattatgtt tattatgagc ttaacacaac
    10381 cctgtttgac gaactcataa tgtggcttaa acaattttag gagttgattt tatgaaaaag
    10441 ctgaataaaa ttatgcttgc actttccata ttgcccactt tgcttacttc cgtattttta
    10501 ttctttatga atgacaccgt tcccatgcac tacaacgcaa gcggtgctgt tgaccgttac
    10561 ggttcaaaat acgaaaactt cattttccct gcaatcattc tgtttatgta tgcattttac
    10621 atggtgtata ccaagatata tactaaatac agcacaaaca gcgttgaaaa gataaataac
    10681 aatgtaaaaa tcactgcgat acttgtgaca tcaatcattg cggctttcaa tattcttcaa
    10741 ttgatgtttc tcattctggc agttttaaag cccgacagca caacttttac agataatgtg
    10801 ccgttcatta taaatacggt tatgggttta atgttgatta ttgcaggcaa ctttcttccg
    10861 aaaacaaaac gaaattcgtt tatcggagta aaaacatcat ggactcaaag cagtgacttg
    10921 gcgtggtatg aatcaaaccg agccggcgga attgcctttg tagttaccgg agttttgaca
    10981 atcattgaaa gctcattcat aaagggaatc aattcaacat ttattatggt tgcaattctt
    11041 ctgataagca ttgccgttgc ctacatctat gcgtatgtta aagtgaaaaa atccgcttaa
    11101 tcaagcacaa aaccgctgtc gggattaaac ctgacagcgg ttgttttttg aaatatttac
    11161 ttataaatat ccactaaaat tactgctctg ctttttcggg gagcgacaaa gctttgcctt
    11221 tatcattttt ctttgtcagc ggagtcatac tctttgcaca aatcatcata gttgacgcat
    11281 tatgcaaaaa tgccgaaacc gacggctgaa taacgcccat aaagcctgaa agcaaaagtg
    11341 ccgaattgaa agcaattatg aaacgatagt ttttgttgat tcgtttcata aggtctttgc
    11401 tcaacttgcg aacacgcaca agcgcacgca aatcagagcc ttttatggta atatccgcag
    11461 tttcacgggc aatgtcggac gcatcgttca ttgcaaccga aacatttgcg gcggcaagtg
    11521 ccggagtatc gttgataccg tcaccgacca taattacctt ctgaccgttc tgcttcattt
    11581 cttcaacata tctgtgcttg tcctcgggga gaacctgaca cttatagtcg gtaattccga
    11641 gcattgcggc agtcgccttt gcggctctgt agctgtcgcc cgtgagcata acaacattct
    11701 ttatgccctg ttttttgagc atatcaattg cctgttttgc ttcttttcga ggcggatcgc
    11761 tgatgcacaa tgcacccgaa agttcaccgc cgattgcaag gtaaagcacg gaacatgagc
    11821 cggatttttc gtcaataata ttctgctgtt cctttgtaac ggtaacattt tcgtcctcaa
    11881 caacaaaatg cttactgccg attattgcac gcttaccgtg aagtgttgtt gcaataccgt
    11941 gggcaacaac atactcaacc tcggtatgct cctcggagtg agaaattcct cgtttttcag
    12001 caccgattac aacggcattt gctacgctgt gcggaaaatg ctcctcaaga caggctgaga
    12061 ttctgagaac ctcgtcctcg gtataatcgc cgaatgcaac aaccttttca agaacaggct
    12121 ctgcgtttgt gagagtacct gtcttgtcaa acacgattgt gtcagcattt gcaaaagcct
    12181 caagatactt tccgcccttg attgtaatat cgttgtcagc cgcctctttg acagccgaaa
    12241 ttacggcaat aggggttgac agcttgattg cacaggagta gtcaaccata agaagtgaaa
    12301 ctgcccttgt gatgtttttt gtgaagataa gagttgcgaa aaatccgata aagctgaacg
    12361 gaacaattct gtcggcaaga gcttcggcac ggctctgaac acctgccttg agttcctcgg
    12421 aattgccgat aagctcaata atcttgctga ttttagtatt tgatgcaagc tgacggactg
    12481 ttacaacaat actgccctct tcgattaccg tacctgcata aacggaaatt ccctcacgct
    12541 ttaatacagg caacggctcg cctgtcattg acgactcgtt tacggttgcc tcaccgtctg
    12601 caacaacacc gtcaacagga ataaccttgc ctgtctgaac acggattttg tcgccgacac
    12661 gcagatcctc aatcggaatg agaacatcac cgtcatctgt aacaagccat accctgtcgg
    12721 tcttgattgc aaggctgtct gtgagaaccg cctttgtcct tgcgtgagta tattcttcaa
    12781 gcaagcccga tattctgagc ataaacataa ccgttgcggc tgttttgaac ttgcgttgac
    12841 acaagcatgc cacaacagac gcaccatcaa gcacatctac ggtaagttta ccgttccaaa
    12901 gagttttgag agccttttta atatattttg cacttctgta aagagtgatt gccgttctta
    12961 tcggagcagg aaggaacatc tttgaaagat agtgctttgc aacaagagtg aaaagattgt
    13021 catgaaaatc gctgtcaatc ttctgaattc caaattctgc actcggttca ttctttttaa
    13081 gagtttttac attaattgct cttacaaggt caataatttt acttctgctg ccctttgtgt
    13141 aattgacaag aataccgccg tttgcagaac ttacctgtgc cgaattcaca taaggagatg
    13201 cggtcacaag attatagatt gccccctcat actccttatc aaatgcatat gcgccgcagc
    13261 gaaaacgaat tctctgcggc ttgtcataga caattttaaa tttcattatt ctgtcacttc
    13321 ttcagcggca gcatcttcgt gaatacctgc ctctgtttta gcatcatagc agatatccga
    13381 agcttcatcc ttcatattct ggaaagctgc ctttgcatct ttctgaagct tcataccctt
    13441 tgcaagacct gtaactgcaa gactgcgggt tttatctgct ttaagtatct ttttgcctac
    13501 gattactgct gctgcgcctg caactgcaca ccataatttt tcgttcttga atactttcaa
    13561 catataaagt cctcctttaa cgctcatatg cacaaatgta catatattta agttgtttta
    13621 cacaacaact ttaccattaa ttatataata tatgacaaat ttgtcaagaa tttgcagagt
    13681 gaaattttcc gattttataa aaaatttatc cgagagcaac atcaagaacc atcatcagcg
    13741 taaatcctat tgcaacaccg attgttccga tgttggaatg tttgccgttt tgtgattccg
    13801 gaataagctc ttcggttaca acatatatca ttgctcctgc ggcaaatgag agaagatacg
    13861 gcaacagcgg aactgctatt cctgcaaaaa tcaccgtaag tacagcaccg ataggctcta
    13921 caactcccga cataattccg agaaagcatg actttttgcg tgacgctccg cctgcatgga
    13981 gcggaagtga aattattgcc ccctcgggaa aattctgtat tgcaatacct accgcaagag
    14041 ccaatgcgcc tgcaacggtt attgccgtat tttccctccg ggatattgtg caaggtaacg
    14101 gcaagaacga gcatagtgtt ctttgaaagg cttgaacgaa cgccctcggg attgtcgctg
    14161 tcaaggtgca tatgcggtat aacgctgtcg agcaaaagca aaaatccaat tccgagcaaa
    14221 aatccgactg tcgcaggaat ccacgcaact atgttctgac tttctgccat ttcaattgac
    14281 ggaattaaca acgaccatat tgatgccgca atcataactc ccgatgcaaa tccgagcaaa
    14341 aatttttgca gcttttgatt gagctcacct tttgaaaaaa gtacaacggc tgagccgagc
    14401 gatgttccga gaaacggtat taaaattata atcagtccgt ttaaaagcat attgacctcc
    14461 ttgtagttag tctttgctaa ctacattgta gcacaacaaa ttttaaaata caatagcgga
    14521 acagaaaatc tctgctccgc tgcataatat gatgtgatat gttaatctgt aataattttt
    14581 attgaactgc ccgactttgt gccgacaacc tcgattttgt tgcctatgct gtttttcagc
    14641 tcgcttatgt gggaaatgat acccacaagg cggttgccgt tgcctgtaag gtcattcagc
    14701 gtttcaattg cagatgcaag gctgtcgttg tcaagtgaac cgaatccctc gtcaataaag
    14761 attgaatcaa gtctgattcc gccgtttctc tgctgaataa tgtcggaaag tccgagtgca
    14821 agcgcaagtg acgcctgaaa agattctccg cccgaaagag atgaaatatc cctttccttg
    14881 ttgttaaaat tatcacgaac gctgatttca agtcctgagc tgattcgctt gtcacggctt
    14941 tcaccacgga caatttcata gcgaccgcca gtcatttttg aaaaacgcag attagcatag
    15001 tacaaaacct gttcaaaata cgaacccata atgtagcgtt caaaggtgat tttgctgtta
    15061 tcggaaacaa tttcaccgtt ggcggttttc ttgagatttt cgtaaacatc ggctattctg
    15121 agcttttctt caaagtcatt tttcagcttt tcaacgctgt tgtaaatgtt ttgattattg
    15181 tgcagacttc tgtcgcctgc ggtataaaga ttgtcccatt tttccttttc agaagtatag
    15241 ctatcaatca gttcattgat tttaaggaca tcttcccttt gctttccttc aactgatttt
    15301 tcacaggctt caaccgttgc ccgagatttt ttcagagaat cggtaaaggt ttctgcctct
    15361 cgttttaatt cttttataga atcttcggtc aacaaattaa aatcgggaca ttccaagata
    15421 ttattctctg aaagaacaat atcaagctcc gattttttgt tttcaagttc agattcaaaa
    15481 cttttcagct gttttgcagt ttcgttttca acagaaacct tgctgtcaaa atctgacttt
    15541 gcctttgaaa gctcgctttg cgcaaggtcg atttccgact taatctgctc tgccttgtcg
    15601 gcacaagcct ttgcagcatt caaaagttct tcaaccgatg agtattttat ctcagcttta
    15661 aggctctcgg tttcttcttt tgcggaatta tatttgcttt ttgcgtcagc atttttgcgg
    15721 attaattctg tatgaacaag gtcaagcttt ttaagctttt catcaaacct tgagatttca
    15781 gccttgcaga cctcacgctg ttctctgact ttttcaaggt cagaagcttc gttttcaagc
    15841 gcttttaata gcttcttttg ttccgtcaaa agctgtgaaa tcatttcggc tgaaagtgtt
    15901 tcggcatcaa cctcaagtgc attcgcaaat tccgtgacat ttgtttttgc ggattctgat
    15961 tccgtttcaa gccttgtgac ctcatttgac tttttatgaa cggcattttc agcaacttca
    16021 caccttgcct tcaaagcatc gagcgtatcc ttgtcggggg cattttcgct gtgttttgcc
    16081 ggatttggat gatgcaatga accgcagacg ggacacggtt tttcatcttc gagtgtgtcg
    16141 gcaagcacgc ctgccatatt cattatatag atattgtgct tagtgttgta ctcgttgttg
    16201 agtgccgttg ccgactttgc aaggtcggca cattctgctt ttacattagt caactttgca
    16261 ttgatttttt cgagattttc atattccgca aaaagccttt cagccttgcc tgatttgtct
    16321 ttcaatgcgg ttattttgct cttggcgtca aagattttct gctctgtgtc gctgttttct
    16381 gagataaatt tttccgcttt tgtctttaga ttttccgtat ccgaataaga ttttctgttg
    16441 cttgaaattt ccttttcgga attttgcaaa actgattcat atacactcaa atttgccgta
    16501 aggttaaaat attttctgta agtatcttcc tttgcacgga gttttcccga ttcctcgttt
    16561 agcgttgcga ttttgccctc aagcgacttt ttctcggcaa aaacattctg ttttaatttc
    16621 aaattttctt cggcggattt tctgtttatt tccgcatttt tacagttttt gagtgccgaa
    16681 tcatatcttt tccgtgactc ggcaaagctg tcatattttg gctttacgga gtgcagtatt
    16741 ttttcgtcat tctcagcttt tttgagcatt tcatcgtaat gcggtttgct ctgcaaaagc
    16801 tgttcgaggc ggagcttttc ggcatcaaga gtatcgaatt tattattaat ttcaactgca
    16861 agagcctttt tcttttcttc ctcaagaatt gtcttttcaa gctcttttcg ctttttttca
    16921 atttctgcat tgtccttttt ctgcatctcg gaaagtaccg ataggtgcaa aagaaattgc
    16981 tcaatatcaa ttaccgtaag gtctgttctg tcggtataaa ggctgatttt ttcgctgatt
    17041 tcggcaaagc tgtcatcatc gggaatttca acggcaacgg tttttgactt gataagttcg
    17101 cagacttttt tcttttcttc aagcttttcg gctgactctg ttttcaattt atcctgaaat
    17161 tctttgtaga tgtccgtgtg aaaaattttg cgaaaaatct cgcttcgctt ttcacttccc
    17221 gactcaatga actttttgaa ttcgccctgt gcaagcattg caatctggcg gtactcatca
    17281 catttcaagc cgataatttc gctgatttta ttattgacct cgcccgtttt tgtgagcgga
    17341 gttgtaatgt catcaccggt aagctctgct gacgcaccct caattttact gcctgttcca
    17401 cgcttttttg cgacctcatt cgaggtggtt ctgatgatgc ggtacttctt tcccctgtgt
    17461 tcaaagtcaa gctcaacata ggtgcgtgtg agcggtttt
//
LOCUS       DAOMVE010000020        16713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_687_length_16713_cov_3.637231, whole genome shotgun sequence.
ACCESSION   DAOMVE010000020 DAOMVE010000000
VERSION     DAOMVE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 16713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16713
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_687_length_16713_cov_3.637231"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1047)
                     /locus_tag="PLS64_02175"
     CDS             complement(211..1047)
                     /locus_tag="PLS64_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRL42673.1"
                     /translation="MFCKNCGRQLLPNEKFCADCGAPVPVQESAVQNEQPIAENQSVE
                     QTADMNVRNEQTQPVENHQTDAYNQMPANDYSAPQYQTVNAQGGYNQYQNGQQGGYSY
                     DYNNQGVQPQYAQPIPKKKSKTPFIITACGIAVALVVAIAAFFIFNGLNKATPQGQIQ
                     GTWIAEENGVSLQLEFKPDGTIGIPNAKELGELISNYVGSSEYSDIINYDEIFNLIDM
                     KYSIDDEKTLSISLEVSFVNQSQSQSLKWSDDPANGGSDTWGIDGDTLYIAGTKLTRI
                     NN"
     regulatory      1297..1406
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1458..2279
                     /gene="thiM"
                     /locus_tag="PLS64_02180"
     CDS             1458..2279
                     /gene="thiM"
                     /locus_tag="PLS64_02180"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRL42674.1"
                     /translation="MIGNCLENVRKNVPLVHNITNYVTVNDVANVLLACGGSPIMSDE
                     PDDVADITSICGGLNINIGTLNKNSIEGMFIAGKKANELSHKILLDPVGAGASKLRTD
                     TAVKLAKEIKFDVIRGNISEIKTLALGSGTTKGVDADVSDAVTEENLDNAVAFVKDYA
                     KKSGCVIAITGAIDLVSDGEKCYVIRNGRPEMSKITGTGCQLSGMTTAFIVANPDNVL
                     EATAAAVCTMGLAGEIAFANMAETDGNSTYRNRIIDAIYNMDAETLEKGAKYELR"
     gene            2266..2910
                     /gene="thiE"
                     /locus_tag="PLS64_02185"
     CDS             2266..2910
                     /gene="thiE"
                     /locus_tag="PLS64_02185"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRL42675.1"
                     /translation="MNCDKKDLLLYAVTDRAWLNGETLCSQVEKAIKGGATFIQLREK
                     NLDQKHFLEEALEIQKLCKKYNVPFVINDNVEIAKEIDADGVHVGQSDMEAGNVRKIL
                     GDDKIIGVSAQTVEQALLAQKHGADYLGVGAVFKTGSKDDADDVSHDELQKICEAVDI
                     PVIAIGGISSKNVTELKGRGIVGIAVISAIFAQPDITKATAELKALTEKTVAND"
     gene            2903..3565
                     /locus_tag="PLS64_02190"
     CDS             2903..3565
                     /locus_tag="PLS64_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRL42676.1"
                     /translation="MIKSAIFDVDGTLLDSMKIWDDAGERYLSSVGKTAENGLSEKLC
                     DMSLTEGAEYMKKQYALSFSTDEIVSGVLKIIEDFYFYEVGLKNDAKEILQFLESNNI
                     KMIIATSSDKTHIKKAFERLGILKYFTDIVTCSQIGKGKTSPDIYLACADKLGTAPSE
                     TLVFEDAVFAAETAHKAGFKTVGVYDESSRNDKNRIKAVCDYYADSFEKAADWGHHLL
                     SL"
     gene            3592..4410
                     /gene="thiD"
                     /locus_tag="PLS64_02195"
     CDS             3592..4410
                     /gene="thiD"
                     /locus_tag="PLS64_02195"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRL42677.1"
                     /translation="MKTALTIAGSDCSGGAGIQADIKTMITNGVYAMSAITALTAQNT
                     TGVTAIMESTPEFLGEQLESIFTDIYPDAVKIGMVSSSSLIKTISDKLKKYDAKNIVV
                     DPVMVATSGAKLISDEAVSTLKEHLFPLATVLTPNIPEAEVLSEMKISNEEEMIKAAE
                     IISEKYGCAVLCKGGHQINDANDLLFKDGTYTWYNGKRINNPNTHGTGCTLSSAIAAN
                     LAKGFDLDASVKRAKDYISGALAAMLDLGKGSGPMNHAFDIQSKFTKENSEAEQ"
     gene            4407..5603
                     /gene="cytX"
                     /locus_tag="PLS64_02200"
     CDS             4407..5603
                     /gene="cytX"
                     /locus_tag="PLS64_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HRL42678.1"
                     /translation="MKKQTSVFENSLIWFGAGVSIAEILTGTYFAPLGFEKGILAVII
                     GHIIGCAMLFCAGLIGGKCRKSSMETVKMSFGNKGGLLFSVLNVIQLVGWTAIMIYDG
                     SLSVNSILNMGDTGRWIACIVIGALIVLWILVGISNLGKLNTIAMAALFILTIVMCFF
                     IFGNGNGMGAAGDDSMSFGAAVELSVAMPLSWLPLISDYTREAEKPFKATLASTLVYG
                     AVSCWMYIIGMSASILTGESDIAKIMLKSGLSIAGLVIVILSTVTTTFLDAYSAGISS
                     ESIFSKLKGKYVAVAVTIVGVIAAIVYPMDDITDFLYLIGSVFAPMIAIQIADFFILK
                     KDRASKAVDICNIIVWLAGFAAYRFLMNVDIIVGNTLPDMVFTIILCVIVTKIADKIT
                     SKSKKA"
     gene            complement(5659..7062)
                     /locus_tag="PLS64_02205"
     CDS             complement(5659..7062)
                     /locus_tag="PLS64_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL42679.1"
                     /translation="MFIGRKNELLQLNRLYNSGKFEFAVIYGRRRVGKTAIINEFVKD
                     KNAICFTGVETNAKQNLENLSECILEFSTGMKTDTAFSNFQSALEYVFKLSENKRIVL
                     CLDEYPYVARASRSLASTLQMLIDKYKDSSKLFLILCGSSMSYIEDHVLAYKAPLYGR
                     RTAQFKILPFEFCEACEYFNGMSPEDKALAYGLVGGTPQYLLQINNKISIEDNIKNIF
                     LNPVSALYEEPNNLLKQEVREPAIYNAVITAIATGASKISEISTKVGETSSTCVAYVK
                     NLVALGIVRKEVPYGEKSTKKTIYSIEDNMFRFWYRFVPENSSIISRGAVELAYSRIA
                     PYLSDYMGAVFEEICKQYLWKRLLNGDSEIDFTDIGRWWGTNPKTRQQEEIDIIGSAD
                     KSTALFGECKWRNESVDLGILETLVERSKLFNFDKKHFYIFSKSGFTKGCIDKANELG
                     NVSLIAYDDILKYNNEN"
     gene            7347..7889
                     /locus_tag="PLS64_02210"
     CDS             7347..7889
                     /locus_tag="PLS64_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation T C-terminal
                     domain-containing protein"
                     /protein_id="HRL42680.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLRIREGDPLEIYTATDGEV
                     IFKKYSPVGELAEFASQYADVLAKISSMPTIITDRDHVIAAAGVSKREFLERRVTTVL
                     EGYMENRKTYSATQQTLGDIQPIEGIEHYASVIYPIIAAGDVSGAVVMLTGEQTKIPT
                     NAEIKLAQTAAAFLGKQMEE"
     gene            8049..9149
                     /locus_tag="PLS64_02215"
     CDS             8049..9149
                     /locus_tag="PLS64_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42681.1"
                     /translation="MFLMNGFSDKVKQKLGYYVYALADPRDNKIFYIGKGINNRIFQH
                     EEKLDNSNKSNRIKEILSSGNKIKKLIISYGLSEKEAFVAESSLINIMNYIDSQSLTN
                     VVSGHHTAPVITAEDFEKIYGAEILSKEDIFRNLLIVKINSLYKYDMSDSQVMECARG
                     HWIIDTKRAENCDYLIAVNHGLIVGVYENMKWYSSGVETPFYPRLCKENLSRSNRKYC
                     TCQAVNKPNIYINKNIANLVNMTQNPISYINGRKNTAKVLKPYYEKFINNSMDIHDFE
                     MNFGNDLVKMGFKLGSFNDSKYEYNNKNILNITDYKQLKKMLKHTDYSTATSLLISKW
                     RYITHWSYMDYQQEKDLPFFKAVIERIFELSE"
     gene            complement(9576..10076)
                     /locus_tag="PLS64_02220"
     CDS             complement(9576..10076)
                     /locus_tag="PLS64_02220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HRL42682.1"
                     /translation="MKKTLIVIDMQNDFIDGSLGTDEAVKIVPNVRKKIEEYRSNGDE
                     IIFTRDTHGEDYLNTPEGKKLPVVHCVKNTHGWQIADGLDVPDAIHIDKPSFGYTHWD
                     KFCFERIELVGLCTDICVVSNALILKALFPNAEISVDSACCAGVTPETHNSALATMKM
                     CQIDVV"
     gene            10232..10765
                     /locus_tag="PLS64_02225"
     CDS             10232..10765
                     /locus_tag="PLS64_02225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRL42683.1"
                     /translation="MGHMYFVRHGQTVWNVENKICGSTDIDLTEKGHEQAVETGKKIL
                     SENIKADIILYSPLMRAEKTAKHISEITGIPAKAEPRLTEQNFGKYEGTPRNGEEFRI
                     AKRDFVSRYDGGESMLHLAQRIYNLLDDVKASGKVCVLVAHNGIARVVNSYFNDMTNE
                     EYADFGVKNCEILRYDF"
     gene            11368..12705
                     /locus_tag="PLS64_02230"
     CDS             11368..12705
                     /locus_tag="PLS64_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRL42684.1"
                     /translation="MPKITHRKKRLSSFKLIVLGFAGVIVLGALILMLPFSSTAGVVT
                     PFHEALFTSTSAVCVTGLVVQDTGSYWSAFGQTVILLLIQIGGLGVVTVAALFAMLSG
                     RKISLMQRSTMQDAISAPKVGGIVRLTRFIVRGTFLIELIGMIVMLPTFCGNYGIKGI
                     WMAAFHSVSAFCNAGFDILGTAENKYPSLTGYIGNPAINITIMLLIIVGGIGFLTWDD
                     IYTNKWHFKKYRMQSKVILVTTAILIIAPAVFFFFCDFTGLPLAERILASLFQSVTPR
                     TAGFNTADLPAMTGSSKAIMTLLMLVGGSPGSTAGGMKTTTLAVLILSAFSVCRKKDD
                     AEVCGRRIDGSAVKNAAAVAVMYFLLFFVGGIVISTAEGLPLSTCLYETASAVGTVGL
                     TLGITPQLGVLSQLILIVLMYLGRVGGLTLIYAAVSNKNHSGAKLPLEKITVG"
     gene            12721..13368
                     /locus_tag="PLS64_02235"
     CDS             12721..13368
                     /locus_tag="PLS64_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRL42685.1"
                     /translation="MKNILLIGLGRFGRHIALQLNKLGHEVMAVDINEERVNESLTIV
                     TNAQIGDSTNTEFLRSLGIGNFDVCIVTIGGNFQNSLETTSLLKELGAKLVVSRAERD
                     VQEKFLLRNGADEVIYPEKQVANWAAIRYTADHIRDYIEVDEGHAIFEVEVPEEWIGK
                     TVGELDIRRKYSINIMATKENGKINMAVSPETVLTDNITLLVLGAYKELQKCFRI"
     gene            13384..13638
                     /locus_tag="PLS64_02240"
     CDS             13384..13638
                     /locus_tag="PLS64_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42686.1"
                     /translation="MAELLLIVSLLAVFVFGFYLMSRLDRFLEENRKALLKENESCEP
                     SKVLLPHGLSESEILCEIRRFRETHGNVDIILSDGQKNDF"
     gene            13702..15678
                     /locus_tag="PLS64_02245"
     CDS             13702..15678
                     /locus_tag="PLS64_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HRL42687.1"
                     /translation="MKVQTEQSKFDTDEHILVCLSSSPSNARIIHTAAKMAKVLHARF
                     TAIYVQTDTVINDEDQSRLSQNIQLAQRLGAEIVMTHGENVALQISEYVRLSDVTKIV
                     IGQSSARRRGLFGRPTLTEKLISSAPDVDIHIIPDALNYTKSHRRRLFFGAEIPSLRD
                     IAVTVLALAVCTAIGFLFDHLDFPDTNIVTVYILGVLMISVLTKGYLCSIAGSLLSVV
                     LFGFFLTEPRLSFQTYAVGYPVTFAVMLASSVLTGTLASKLKDHARLSARSAFRVQVL
                     FDTDRLLQKAKSNDDVLSVTCMQLSRLLDRSIVAYTKGENGMLSGRLYAEKKDTHAEK
                     LLSDAERQTAEWVLQNGHRAGAATAQFGKSECLYLAIRAGGRVYGVIGIPMKPEKPDS
                     FESSIVLSVVNECALAMDNAHNAAEKERAADLAKSEQLRADLLRSISHDLRTPLCSVS
                     GNADTLLHNGNCLDEATKQQIYKDIYDDSEWLIGVVENLLYVTRLNDGRLKLELTDQL
                     VDEVVNEAVSHLKKKSVGHKVTVRCDDLILARMDAQLIVQVIVNLIDNALKYTEQGSE
                     ICVSAEKQGGDAVIRVTDNGNGIADDMKPHIFEMFYTGKNTVADSRRSFGIGLTLCKS
                     IVELHGGTLTLTDNVPHGCIFTFTLPLSEVTLNE"
     gene            15671..16372
                     /locus_tag="PLS64_02250"
     CDS             15671..16372
                     /locus_tag="PLS64_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL42688.1"
                     /translation="MNKPLVLVVEDDRPIRNLIVTTLKAHDYHYLTAENGHSAIIEAT
                     SHNPDIVLLDLGLPDIDGTQVIESIRSWSNMPIIVISARSEDKDKITALDAGADDYLT
                     KPFSIEELLARLRVTQRRLLLLQASGDADSPVFQNGKLKIDYAAGCAYLNGEELHLTP
                     IEYKLLCLLSHNVGKVLTHTYITQQIWGSSWENDIASLRVFMATLRKKLEPQRDCPQY
                     IQTHIGVGYRMLRVD"
     gene            16402..16614
                     /locus_tag="PLS64_02255"
     CDS             16402..16614
                     /locus_tag="PLS64_02255"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRL42689.1"
                     /translation="MCYPHFRRKAYLLPFYMIGYGTIRFFVEGLRSDSLYLLPGIRVS
                     QVLSGCLIILGIVILFIIRKRQAVRQ"
     gene            16611..>16713
                     /locus_tag="PLS64_02260"
     CDS             16611..>16713
                     /locus_tag="PLS64_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HRL42690.1"
                     /translation="MRKEWTLFLTFLKIGAFTFGGGYAMIALLENEFV"
BASE COUNT         4931 a         3554 c         3647 g         4581 t
ORIGIN      
        1 acagaaattg tgctgtcttt tctttttgta caaatctgta ggagcagata ttatccgccc
       61 gcaagtaagc gcacatattt tgtgattgtc atacagtatc gttcatcgga caacgacgga
      121 atccctttga cagagtatgt atattccaca caacaaaagg tcacgatttt gaaccgtgac
      181 cttgtttttt acgaattttt taagacaata tcagttattg attcttgtca gctttgtgcc
      241 ggctatgtag agagtatcgc cgtcgattcc ccatgtatcc gaaccgccgt ttgcgggatc
      301 gtcagaccat ttgagtgact gcgactgact ttgatttaca aaagacacct caaggctgat
      361 tgaaagagtt ttttcatcat caatgctgta cttcatatca atcaaattaa aaatctcatc
      421 atagttaata atgtcgctgt attccgagct gcctacataa tttgaaataa gttcgccgag
      481 ttcttttgca ttcgggatac cgattgttcc gtcaggcttg aactcaagtt gtaggcttac
      541 tccgttctcc tcggcaatcc atgtaccctg aatctgtccc tgaggagtgg ctttgttaag
      601 tccgttaaat ataaagaatg ccgcaattgc aacaacgagt gcaaccgcaa tgccgcacgc
      661 tgtaattata aacggtgtct ttgacttttt ctttggaatc ggctgtgcat actgtggctg
      721 aacgccctga ttgttataat cataagaata tccgccctgc tgaccgttct gatactgatt
      781 atatccgccc tgtgcattaa ctgtctgata ctgcggtgcg gaataatcat ttgcaggcat
      841 ctgattatat gcgtcagtct gatgattttc aacaggctgt gtctgctcat tccgaacatt
      901 catatcggca gtctgttcaa ccgactgatt ttctgcaatc ggctgttcgt tctgtacagc
      961 tgattcctgt acgggtacag gcgctccgca atcggcacaa aacttttcat taggcaataa
     1021 ctgtcttccg caatttttac aaaacatact tcttctcctg ttcaacaaaa aataatttcg
     1081 aggaaaaccc ctttacaaca gcaaattcaa ccatattatc tgttattttt attgtactgt
     1141 tttttcttct tcaagtcaac tgatatttaa aaatcagtgt ttttttacat aaaagatata
     1201 ctgaatacag aagattttac tgaggataga ttcgtactaa tcgttctcgg tgactatttg
     1261 tcaagttttc attgacaaat tacgacacaa attgtatatt ataagtaagg gagctgacaa
     1321 gttagtcggc tgagaggagg ctgaatgcct cgacctatga cctgatttgg ataatgccaa
     1381 cggagggaaa cagcgagcct ttacggacac accttttgtt ggtgcgtccg ttttattttt
     1441 ataaaggaga atccgctatg attggaaatt gtcttgaaaa tgtaagaaaa aatgtaccgc
     1501 ttgtacacaa catcacaaac tatgtaacag taaacgatgt tgcaaatgta cttctcgctt
     1561 gcggaggaag tccgattatg tcagacgaac ccgatgatgt tgccgacatc acaagcattt
     1621 gcggcggtct taacatcaac atcggtacac ttaacaaaaa ctcaattgag ggtatgttca
     1681 ttgcaggcaa aaaagctaac gaactttctc acaaaattct acttgatcct gtcggcgcag
     1741 gcgcaagcaa gctccgcaca gacacagcgg ttaagcttgc aaaagaaatc aaatttgatg
     1801 ttatcagagg taacatctcg gaaatcaaaa ctcttgcact cggaagcggc acaacaaagg
     1861 gtgttgatgc cgatgtatcg gatgcggtaa cagaagaaaa tctcgataac gcagttgcat
     1921 ttgtaaagga ttatgccaaa aagtcgggat gcgtaattgc aatcacaggc gctattgacc
     1981 ttgtaagtga cggcgaaaag tgctatgtaa tccgcaacgg tcgtcccgag atgagcaaaa
     2041 tcacaggaac aggctgtcag ctttcgggta tgacaactgc atttatcgtt gcaaaccccg
     2101 acaatgttct tgaggcaact gccgcagcag tttgtacaat gggacttgca ggcgaaatcg
     2161 cttttgcaaa tatggcagaa acagacggca actcaacata ccgcaaccga attattgacg
     2221 ctatctataa tatggatgcc gaaacacttg aaaaaggagc aaagtatgaa ttgcgataaa
     2281 aaagatttgt tgctctatgc cgtaaccgac agggcatggc ttaacggtga aacactttgc
     2341 agtcaggttg aaaaggctat taaaggcggc gcaacattca ttcagttaag agaaaaaaat
     2401 cttgaccaaa agcattttct tgaagaggct cttgaaattc agaaactctg caaaaagtac
     2461 aatgttccct tcgtaatcaa tgacaatgta gaaattgcaa aagaaattga cgctgacggt
     2521 gttcatgtcg gtcagagtga tatggaggca ggcaatgtcc gcaagattct cggtgacgac
     2581 aaaatcatcg gtgtttcggc tcagactgtt gaacaggctc ttcttgctca gaagcacgga
     2641 gcagattatc tcggagtcgg agcagttttc aaaacaggct caaaggacga tgcagacgat
     2701 gtttcacacg atgaacttca gaaaatctgt gaggctgttg acattcctgt aatcgcaatc
     2761 ggcggaatca gctccaaaaa tgttacggaa ctcaagggca gaggtatcgt aggcattgcc
     2821 gttatcagtg ctattttcgc tcagcccgat attacaaagg caacagcaga gcttaaagca
     2881 cttacggaaa agacggttgc aaatgattaa atctgcaata tttgatgttg acggcacgct
     2941 tctcgattca atgaagatat gggatgacgc aggagagcgt tacctctcgt ctgtcggcaa
     3001 aacagccgaa aacggacttt ccgaaaagct ctgtgatatg agtctgacgg agggtgcgga
     3061 gtatatgaaa aagcagtatg ctctttcctt ttcaactgat gaaatagttt cgggtgtgct
     3121 gaaaatcatt gaagattttt acttttatga ggtcggttta aaaaacgatg caaaagaaat
     3181 tttgcagttt ttggaatcga acaatatcaa aatgattatt gcaacatcaa gcgacaaaac
     3241 gcatattaaa aaggcatttg aaaggctcgg tattctaaaa tattttacgg acattgtgac
     3301 ctgttcacaa atcggaaaag gcaaaacaag tcccgacatt taccttgcct gtgcagataa
     3361 actcggaaca gctccgagtg aaacgcttgt attcgaggat gctgtttttg ccgcagaaac
     3421 tgctcacaag gcaggtttca aaacggtggg agtgtatgac gaatcaagca ggaatgataa
     3481 gaacagaata aaagccgttt gcgattacta cgcagacagc tttgaaaaag cggcagattg
     3541 ggggcaccac cttttgtcgc tgtaaaaaat taatgcaaag aggagattaa aatgaaaaca
     3601 gcattaacaa tcgctggcag tgactgcagc ggaggcgccg gtattcaagc ggatatcaaa
     3661 actatgataa caaacggtgt ttatgccatg agcgcaatca ccgcacttac cgcacagaac
     3721 acaaccggcg ttacggcaat tatggaatcc acacccgaat ttctgggtga acagcttgaa
     3781 agtatcttta cggatattta tcccgatgcc gtaaaaatcg gaatggtttc ttcatcaagc
     3841 cttatcaaaa caatttcgga caagctgaaa aagtacgatg caaaaaatat tgttgtcgat
     3901 ccggttatgg tggcaacaag cggagcaaag cttatcagcg acgaggcggt aagcactctc
     3961 aaagagcatc tctttccgct tgcaacagtt cttacaccga atattccgga ggcggaagtt
     4021 ctctctgaaa tgaaaatttc aaatgaggaa gaaatgataa aagccgctga aataatcagc
     4081 gaaaagtacg gttgtgcggt tctctgcaag ggcggacatc agattaacga tgcaaacgat
     4141 ttgctcttta aagacggcac ctacacatgg tacaacggca aaagaatcaa caatcccaac
     4201 acacacggaa caggctgtac actttcaagt gcaatagccg ctaatcttgc aaaaggcttt
     4261 gaccttgacg cttccgtaaa gcgtgcaaag gattatattt caggcgcact tgcggcaatg
     4321 cttgacctcg gcaaaggcag cggtcccatg aaccacgctt ttgacattca aagtaaattt
     4381 acaaaagaaa attcggaggc agagcaatga aaaagcagac aagtgtattt gaaaacagtt
     4441 tgatatggtt cggagcaggc gtttcaatcg ccgaaattct cacaggtaca tactttgctc
     4501 cgttaggttt tgaaaagggc attttagccg taatcatcgg tcacataatc ggatgtgcaa
     4561 tgctcttttg tgcaggactc atcggcggta agtgccgtaa aagctcaatg gaaaccgtaa
     4621 aaatgagctt tggcaacaag ggcggacttc ttttctctgt cctcaatgtt attcagcttg
     4681 tcggctggac agccattatg atttatgacg gctcactttc cgtgaacagt attctgaata
     4741 tgggcgacac aggcaggtgg attgcctgta tcgtaatcgg tgcattgatt gtactttgga
     4801 ttcttgtcgg catttcaaac ctcggcaagc tcaacacaat cgcaatggcg gcactgttca
     4861 ttctcacaat cgtgatgtgt ttctttattt tcggaaacgg caacggtatg ggtgcggcag
     4921 gcgatgactc aatgtcattc ggtgcggcag ttgagctttc ggtagcaatg ccgctttcat
     4981 ggttaccgct catcagcgac tacacaagag aggcggaaaa gccgttcaag gcaactctcg
     5041 caagcacact tgtttacggt gcggtaagct gttggatgta cataatcggt atgagtgcat
     5101 ctatccttac aggcgaaagc gatattgcaa aaatcatgct taaatcagga ctcagcattg
     5161 caggtcttgt aattgtaatt ctttcaacag ttacaacaac cttccttgac gcttattcgg
     5221 caggcatttc aagcgaatca atcttctcaa aactcaaagg aaaatatgtt gccgttgcag
     5281 taacaatagt aggtgtaatc gccgcaattg tttatcctat ggatgacatc accgattttc
     5341 tctatctcat cggttcggta tttgctccga tgattgccat tcagattgca gactttttca
     5401 ttctcaaaaa agacagagca agcaaagccg ttgacatctg caacatcata gtatggcttg
     5461 caggctttgc ggcatatcgt ttcctgatga atgttgacat tatcgtcggc aacaccttgc
     5521 ccgatatggt tttcacaatc atcctctgcg tcatagttac caaaatcgca gacaaaatca
     5581 cctcaaaatc caaaaaagca taatcgctta caacaaagtc aaatccccgt cagatttctc
     5641 cgacggggat ttttacattt aattttcgtt attatatttt aaaatatcat cataagcaat
     5701 caggctgaca tttccgagtt catttgcctt atcaatgcaa ccctttgtaa aaccggactt
     5761 tgaaaaaata taaaaatgct ttttgtcgaa attaaaaagc ttactgcgtt caacaagtgt
     5821 ttcaagaata ccgaggtcaa cactttcatt acgccactta cattcaccaa aaagagccgt
     5881 acttttatct gccgagccga ttatatcaat ttcttcctgt tgtcttgttt ttggatttgt
     5941 tccccaccat ctgccgatat ccgtaaaatc aatttcggaa tcaccgttca gcaacctttt
     6001 ccacaaatac tgcttgcaga tttcttcaaa cacagcaccc atataatccg acaaatacgg
     6061 tgcaattcga ctgtacgcaa gttcaaccgc acctcttgaa attatcgaag aattttccgg
     6121 cacaaaacga taccaaaatc tgaacatatt atcctcaata gaataaattg ttttctttgt
     6181 ggatttttca ccgtacggca cttcttttct cacaattccg agagcaacaa gattcttgac
     6241 ataagcaaca caagtgcttg aggtttctcc aacctttgtt gaaatctccg atatttttga
     6301 tgcacccgta gcaattgccg taatcacggc attataaata gccggctctc ttacctcttg
     6361 tttcaaaaga ttgttaggtt cttcatacag agccgataca ggattcagaa aaatattttt
     6421 tatattatcc tctattgaaa ttttgttgtt aatctgcaaa agatattgcg gtgtacctcc
     6481 cacaagcccg taggcaagtg ccttatcttc aggtgacatt ccgttaaaat actcacaagc
     6541 ctcacaaaat tcaaacggca aaatcttaaa ctgagccgtt cttctgccat acaaaggagc
     6601 tttgtatgca agcacatggt cctcaatata tgacattgat gaaccgcaga gaataagaaa
     6661 caactttgaa ctgtctttat atttatcaat taacatttgc agtgttgatg caagacttct
     6721 tgatgccctt gctacatacg gatattcgtc aaggcaaagg acgattcttt tgttctctga
     6781 caatttaaaa acatattcaa gtgccgactg aaagtttgaa aaagcagtat ccgttttcat
     6841 tcctgttgaa aattcaagaa tacactcgct caaattttca agattttgct ttgcatttgt
     6901 ttcaactcct gtgaaacaaa ttgcgttttt atccttgaca aactcgttta tgatagccgt
     6961 ttttcctaca cggcggcgac cgtatatgac ggcaaattca aattttccgc tgttatacaa
     7021 tctgttaagc tgtaaaagct cattctttct gcctataaac aaaattctca cctctgaccg
     7081 tttgcaaatt actcatttac gatttagtta tttacgatta actaaattat aaaccactgt
     7141 ttttgctaag tcaagattat tttgcaattt agttggttac gattaactaa atcgtgataa
     7201 agttatattt tttatatgtt gtaaaaatat gtcaccaaag aaaatttcgg tataaaaaaa
     7261 ctgtatgaat caaacaatat tactgcaaat aatttgctga atatgtatca gacaaagggc
     7321 aaaattgtcc gaagggagtt tttaatatga aagcaacagg aatcgtcaga agaatcgacg
     7381 atctcggaag agtggttata ccgaaagaaa tcaggcggac gcttcgtatt cgtgagggag
     7441 atccgctcga aatttatacc gcaacggacg gcgaggttat ttttaaaaaa tattcgcctg
     7501 tcggagagct tgcggagttt gcgtcacaat acgcagatgt gcttgccaaa atcagctcaa
     7561 tgccgacaat cataacagac cgtgaccatg taatcgccgc cgcaggggtt tcaaagcgtg
     7621 aatttcttga gcgcagggta acaaccgtgc ttgagggata tatggaaaat cgcaaaacct
     7681 attcggcaac acagcagacg ctcggcgata ttcagccgat tgagggcatt gaacattatg
     7741 catcggtaat ctatccgatt atcgccgccg gagatgtttc gggtgcggta gttatgctca
     7801 caggcgaaca gacaaaaatc ccgacaaatg ccgaaattaa gcttgcacaa accgccgccg
     7861 cattcctcgg caagcaaatg gaagaatgat ttttaaaaat aggtaataat aaaaaatgcg
     7921 gtcgggaaga caggtgtaaa acaatacacc gaattttccc gaccgtattt gtataaatca
     7981 agtacacaaa acaacgcttt accgtattta acatttatgg tatcattaaa taaattaagt
     8041 tgaaaggtat gtttcttatg aatggctttt ccgataaggt aaaacaaaaa ttaggttact
     8101 atgtttatgc ccttgcagat ccaagagata acaaaatatt ttacataggc aaaggaatta
     8161 acaaccgtat ttttcaacac gaagaaaaac tcgataactc aaataagtcg aatcgtataa
     8221 aagaaatact ctcatcgggt aacaaaatca aaaaactaat tatatcatat ggtctttcgg
     8281 aaaaagaggc attcgttgcc gaatcgtctt tgataaatat aatgaactac attgactctc
     8341 aatcactgac taatgtagtt tcgggacacc acacagcacc tgttattaca gctgaagatt
     8401 ttgaaaaaat ttacggtgcc gaaattcttt caaaagaaga tattttccgc aatcttttaa
     8461 ttgttaaaat aaattcactt tataaatacg atatgtcaga cagccaagtt atggaatgtg
     8521 cgagaggtca ttggataatc gacaccaaac gagcagaaaa ttgcgactat cttattgctg
     8581 taaatcacgg actgattgtc ggagtttatg aaaatatgaa gtggtattct tcaggtgtgg
     8641 aaactccatt ctatccacga ttgtgcaaag aaaatctttc aaggtcaaat cgcaaatact
     8701 gcacctgtca agcagttaat aagccgaata tttacataaa caaaaacatt gccaatttag
     8761 taaatatgac gcaaaatcct atttcataca taaacggaag aaaaaatacc gctaaggtgc
     8821 taaaaccata ctacgaaaaa ttcatcaata attctatgga tattcatgat tttgaaatga
     8881 atttcggtaa tgatttggta aaaatgggat ttaaattagg cagtttcaat gattcaaaat
     8941 atgaatataa taacaaaaac attttaaata ttaccgatta caaacagctt aaaaaaatgc
     9001 tgaaacatac ggattacagc acagccacat cattgttgat ttcaaaatgg agatatatca
     9061 cacattggag ttatatggat tatcagcagg aaaaagattt accatttttc aaagctgtta
     9121 tagaaagaat atttgaactt tccgagtagc ttcatacaaa aaattttaca attgatttat
     9181 tttgaataat cttcaatttt ctaaaaaact tgcacataca aaaaataaaa cttcaatctc
     9241 ttgtcaaccg aaattgaagt tttttattga cataattgtt catcaatgat aacatataag
     9301 taaggaaacg gctttagctg tttcgtaagt ttgaacaata aaaattaatt aaagattaac
     9361 cgctctgttc acgacacagg gcggttattt ctttatggtg aacacaataa aaaatgtgaa
     9421 aattactatc acagctatgt attccacgca atcaccccct ctctgataag ggagtcgaaa
     9481 cagccgccac cgtttcctta ccaagtaatt atcgcaaagt attttaatat attcaatatc
     9541 acgaacttaa aatcacaata tattttatta ttaaatcaca ccacatcaat ctggcacatt
     9601 ttcattgttg cgagggcgga attgtgggtt tcgggggtta cgccggcaca gcaagccgaa
     9661 tcaacgctga tttcagcgtt cgggaaaagt gcctttagaa tcaacgcatt tgacacaacg
     9721 cagatatcgg tgcaaagtcc tacaagctcg attctttcaa agcagaattt atcccaatga
     9781 gtatagccga atgacggctt gtcaatatga attgcgtcgg gaacatcaag tccgtctgca
     9841 atctgccagc cgtgagtatt ttttacgcaa tgtacaacgg gcagtttttt gccctcggga
     9901 gtgtttaggt aatcttcacc gtgagtatcc cttgtaaaaa taatttcatc accgtttgaa
     9961 cggtattctt caatcttttt gcgtacatta ggcacaattt tcacagcctc atcagtaccg
    10021 agactgccgt caataaaatc attctgcata tctattacaa tcaaagtttt cttcatacaa
    10081 atcactccgt ttttaatttt gaactgtaca tatcaaatat atcagctgac tttgccgctg
    10141 tcaatttatt tgcaaaaatt tgttttgtaa cttgattttc gttgtcggct caattaaaat
    10201 agtgtttaga aaaactgcaa ggagaacgct tatgggacat atgtattttg tcagacacgg
    10261 acagaccgtg tggaatgttg aaaataaaat ctgcggttca acggatattg acctcacgga
    10321 aaaaggtcat gaacaggctg ttgaaacggg caagaaaatt ctttcagaga atataaaagc
    10381 agatatcatc ctctattctc cgcttatgag agccgaaaaa accgcaaagc acatttcgga
    10441 aatcacggga attcctgcaa aagcagagcc gagattaacc gagcaaaact ttggaaaata
    10501 tgagggaacg ccaagaaacg gcgaagagtt tcgcattgcc aaaagagatt tcgtcagccg
    10561 ttatgacggc ggagagtcta tgctgcacct tgcacaaaga atttacaatc tgcttgatga
    10621 tgtgaaagca agcggaaagg tttgtgtttt agttgctcac aacggcattg caagagttgt
    10681 caactcatat tttaacgata tgacaaatga ggagtatgca gatttcggtg ttaaaaactg
    10741 tgaaattctg cgttacgatt tttaagtatt tctaaatatc cgcataaaat caaggagtac
    10801 cacgcattgt gacactcata ttttagtgtt gttatgaata ttgacgacag agacagagga
    10861 agaaaaggca tagaagaaat gcttgtgaga atgtcaaaaa tgactgatat gcccgacaat
    10921 cccaagaaaa acaaaacaca aatataaaac ataggcaagg aaaaattagg acgctccaaa
    10981 cggaacatcc ttttacattt atatatagct atttatgagt tgctttgata ctgctggaga
    11041 aaagcctgaa acgctagccg cgatgcaaat cgaaatccgt ttgtgaacgc ttgtagctca
    11101 ttccggtcgt aatttctaca tccgtttgtt aatttgttcg aacagagcct tttgttgttc
    11161 tgtgagagtg gctctgagct ttgtttcaag ccgcacaacg gttacaaacg atccgccttt
    11221 gtctgtgccg tttgctatgc tctgcgctgt ttttttaaag aatgagcgcc ttaatgcaat
    11281 cttaatacag agtgccgaat cattacaccg tttttttata tttcacggta tactctttgt
    11341 aaacaagtag aaacaggaga agtatatatg cctaaaatta cacatagaaa aaagcggctg
    11401 tcatccttta agctgatcgt tttgggcttt gccggcgtca tagtgcttgg agcgctgata
    11461 ttgatgctgc cgttttcatc gactgccgga gttgttacgc cgtttcacga ggcgctgttc
    11521 acatcgacct ccgccgtttg cgttacgggg ctggtcgtgc aggatacggg cagttattgg
    11581 tccgctttcg ggcagacggt aatactgctg ctgattcaga taggcgggct cggtgttgtg
    11641 acagttgcgg cacttttcgc tatgctgtcc ggcagaaaaa tatcgctgat gcagcgcagt
    11701 accatgcagg atgccatctc tgccccgaag gtcggcggga tcgtccgcct gactcgtttt
    11761 attgtgcggg gaactttttt gatcgagctt atcgggatga tcgtcatgct gccgacattt
    11821 tgcggaaact acggtattaa gggaatttgg atggcggcgt ttcattcggt ttccgccttt
    11881 tgcaacgccg gttttgacat actcggcacc gccgaaaaca aatacccgtc acttacggga
    11941 tacataggaa atccggcgat aaacattaca attatgcttt taattattgt gggcggtatt
    12001 ggatttttga cctgggacga tatttacaca aacaagtggc atttcaaaaa ataccgtatg
    12061 caaagtaagg tcatattggt aacaacggca atcctcatta tcgctcccgc agtattcttt
    12121 ttcttctgcg attttacggg gctgccgctc gccgaaagga ttcttgcgtc gctttttcag
    12181 tctgtcacac cgagaaccgc cggtttcaat accgctgatc ttcccgctat gaccggttct
    12241 tccaaggcaa ttatgaccct gctcatgctc gtcggcggct cgcccggctc cacggcaggc
    12301 ggtatgaaaa cgaccacgct tgccgttctg attctgagtg cattctccgt ctgtcgcaaa
    12361 aaggacgatg ccgaagtctg cggcaggcgc attgacggtt cggcagtgaa aaacgccgca
    12421 gccgttgccg taatgtattt cctgctgttt tttgtcggcg gcattgttat cagcacggca
    12481 gaaggtctgc cgcttagcac ctgcctttac gaaacggcgt ccgctgtcgg aacggtcggg
    12541 cttacccttg gaatcacacc gcaactcggt gttctttctc agcttatttt gatcgtcttg
    12601 atgtatctcg gcagagtcgg cggacttacc ctgatttacg cggcggtttc caacaaaaat
    12661 catagtggag caaagttgcc gctcgaaaaa atcacagtcg gataaaggag ttactatatt
    12721 atgaaaaaca tattgttaat aggtctcggc agattcggta ggcatattgc tttgcagctg
    12781 aataagctcg gacacgaggt gatggcggta gacatcaacg aagaaagagt caatgaaagt
    12841 ctgacgattg tcaccaatgc acagatcggc gacagcacaa acacggagtt tttaaggtcg
    12901 ctcggcatcg gaaactttga tgtgtgcatc gtcaccatcg gcggaaattt tcaaaactcg
    12961 ctggaaacca cttctttgct gaaagaactc ggcgcaaaat tagttgtatc ccgtgccgag
    13021 cgtgatgtgc aggaaaaatt ccttctgcgg aacggagcgg acgaggtcat ttacccggaa
    13081 aaacaggtag caaattgggc tgctattcgg tacaccgccg accacattcg ggattacatt
    13141 gaggttgatg agggtcatgc catttttgag gtagaggtgc cggaggaatg gatcggcaaa
    13201 accgtcggag aactggatat ccgcagaaaa tacagcatca atattatggc aaccaaggag
    13261 aacggaaaaa tcaatatggc agtttcaccg gaaaccgttc ttacggacaa tatcactctt
    13321 ttggttctcg gtgcttacaa agaactgcaa aagtgttttc gcatttaaca gaggtgaatg
    13381 aggatggctg agctattgct gattgtcagc ctgcttgccg tatttgtttt cggattttat
    13441 cttatgagca ggctggatcg atttcttgaa gaaaacagaa aggcactgtt aaaggaaaac
    13501 gaatcctgcg agccttccaa agtcctgctg ccgcatggac tgtccgaaag tgaaatatta
    13561 tgtgagatcc gccgttttcg tgaaacgcac ggaaatgtcg atattattct ttccgatggg
    13621 cagaagaatg atttttaggt tgcggtagat ttttttacag ttttactgta taataataaa
    13681 ataaaaaggg cggtgaaacc gatgaaagta caaaccgaac agtcaaaatt cgatacagac
    13741 gagcatatcc ttgtctgcct atcctcttcg ccgtccaatg caaggatcat acacaccgcc
    13801 gcaaagatgg ctaaggtcct tcatgcaaga tttaccgcca tctatgtgca gacggatacg
    13861 gtcataaatg atgaggatca gtcccggctt tcgcagaaca tacagctcgc gcaaaggctc
    13921 ggcgccgaga tcgttatgac gcacggcgag aatgtagcgc tgcagatctc ggaatatgtc
    13981 aggctttccg atgtcacaaa aatcgtgatc gggcaaagca gtgccagacg cagagggctg
    14041 ttcggaagac cgactctcac cgaaaagctg atctcctccg cccccgatgt tgacattcat
    14101 attattcccg acgcactgaa ctacacaaag tcgcacagac gccgtctgtt ctttggtgca
    14161 gagataccgt cgctgcggga tattgccgtg accgtcctgg cgcttgccgt atgtacggcg
    14221 atcgggtttc tgttcgacca tctcgacttt cccgatacca atatcgttac ggtatacatc
    14281 ctaggtgtgc tgatgatctc cgttttgacc aaaggatacc tttgcagtat agcaggctca
    14341 cttctaagtg ttgtattgtt cggatttttc cttaccgaac ccaggttgtc ctttcagacc
    14401 tatgccgtag gctatcccgt tacctttgca gttatgctgg cgtcttccgt tctcaccggc
    14461 acgcttgcct cgaagctgaa agatcatgca aggctctcgg ctcgctctgc gttccgtgtg
    14521 caggtgctgt ttgacactga ccgtctgctg caaaaagcaa aaagcaacga tgatgtttta
    14581 agcgtgacct gtatgcagct ctcgcggttg cttgaccgca gtattgttgc ctatacgaaa
    14641 ggcgaaaacg gaatgttgtc cgggcggctg tatgccgaaa aaaaggatac acacgctgaa
    14701 aagctgctaa gcgatgcaga gcggcagacc gcagaatggg tgcttcaaaa cggtcaccgt
    14761 gcaggagcag cgacagcaca gtttggaaaa tccgaatgtc tttatcttgc aatccgtgca
    14821 ggcggcagag tctacggtgt aattgggata ccgatgaaac ccgaaaagcc cgattcattt
    14881 gagagcagca tcgtactgtc ggttgtgaac gaatgtgcgc tggcaatgga taatgctcac
    14941 aacgccgccg aaaaggaacg ggcggcagat cttgcaaaaa gcgagcagct gcgcgccgac
    15001 cttttgcggt cgatctcaca cgatctgcgc acgccgcttt gctccgtttc gggtaatgcg
    15061 gacacgcttt tgcataacgg aaactgcctg gacgaagcaa ccaagcagca gatctataaa
    15121 gatatttatg acgattccga atggctgatc ggcgtggtgg aaaatctgct gtatgttaca
    15181 cggctgaatg acggacgcct gaagctggag cttaccgacc agcttgtgga cgaagtggtc
    15241 aacgaagccg tttcgcattt gaagaaaaaa tccgtcggac acaaggtgac ggtaaggtgc
    15301 gacgatctga tccttgcccg tatggacgca cagcttatcg tgcaggtcat tgtcaacctt
    15361 attgataatg ctttgaaata taccgaacaa ggctccgaga tctgcgtaag tgcggaaaaa
    15421 cagggcgggg atgccgtgat ccgagtcacc gataacggaa acggaattgc agacgatatg
    15481 aaaccgcata tttttgagat gttttacacc ggaaaaaata ccgtcgcaga cagccgcaga
    15541 agcttcggga tcgggcttac cctgtgtaaa tctatcgtag agctgcacgg cggaacgctg
    15601 acgcttacgg ataacgtacc gcacggctgc atatttacct ttactttacc gctcagcgag
    15661 gtgactctga atgaataaac cccttgtact cgtcgtggag gacgaccgcc ccatccgcaa
    15721 tctgatcgta acaacgctaa aggctcacga ttaccactac ctgaccgcgg agaacggaca
    15781 cagcgctatt atagaagcga cctcccataa cccggatatc gttctgctgg atctggggct
    15841 gccggatatc gacggcacac aggtgatcga aagtatccgc tcatggtcaa atatgcccat
    15901 tattgtgatc agtgcgcgca gcgaggataa agacaaaatc acggcgctgg atgccggggc
    15961 ggacgattat ctgacaaagc ccttttccat agaggagctt ttggcacgcc ttcgggtcac
    16021 gcaaagacgg ctgctgctgc ttcaggcaag cggcgacgcc gactcacccg ttttccaaaa
    16081 cggcaagctt aaaatcgatt atgccgccgg ctgcgcttat ctaaatgggg aggaattgca
    16141 cctgaccccg attgaataca agcttttgtg tctgctctct cataatgtcg gaaaggtgct
    16201 gacgcacacc tatatcacgc agcagatttg gggcagcagc tgggaaaacg atattgcttc
    16261 tctgcgggtg tttatggcga ccctgcgcaa aaagctggag ccgcaaaggg actgtccgca
    16321 atacattcag acgcatatcg gcgtcggtta ccgtatgctg cgggtagact gagaaaagcg
    16381 aggaaacaag gatttgaacc gatatgttac ccgcatttca ggcgaaaagc ctatcttctt
    16441 ccgttttata tgatcgggta cggaacgata cggttttttg tggaggggtt gcgctccgac
    16501 agcctttatc tccttccggg tatccgtgta tcacaggtgc tgtccggctg cctgattatt
    16561 ctcggtatcg ttatactttt tatcatccgt aaaagacagg cggtgcggca atgagaaaag
    16621 agtggacttt gtttctcacc tttcttaaaa tcggcgcctt taccttcggc ggcggatatg
    16681 ctatgattgc gctgcttgaa aacgaatttg tag
//
LOCUS       DAOMVE010000021        15999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_740_length_15999_cov_3.649649, whole genome shotgun sequence.
ACCESSION   DAOMVE010000021 DAOMVE010000000
VERSION     DAOMVE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15999
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_740_length_15999_cov_3.649649"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..360
                     /locus_tag="PLS64_02265"
     CDS             40..360
                     /locus_tag="PLS64_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42691.1"
                     /translation="MARTIINLPLRNSVDESYNTAVNILQANGFKEVSYNGETVWKKG
                     TGLLTAMQYIKIEFTDTTLVVSGWVQAGVGSVGGNEMALNGIVAAVPKKSVMKVIEQI
                     KASL"
     gene            375..758
                     /locus_tag="PLS64_02270"
     CDS             375..758
                     /locus_tag="PLS64_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42692.1"
                     /translation="MIKLRKISMVLCIITMLFTVMSLSGCSNNSITGIWAFVDDDGQV
                     SENSKMYFNDDGSCNDIDYHGPTSADPVSYKVMSDGKLVFTMEWDGTSSFDLAQSEEE
                     AMNDYHKYYISGDTLIFRKSTYKRV"
     gene            complement(811..993)
                     /locus_tag="PLS64_02275"
     CDS             complement(811..993)
                     /locus_tag="PLS64_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42693.1"
                     /translation="MNTSPMMNVMKGVAIGMTAGVCMGIVGKNMVDSNPKLKKKANKA
                     FKTMESVLDTAQYMFR"
     gene            1152..1670
                     /gene="trmL"
                     /locus_tag="PLS64_02280"
     CDS             1152..1670
                     /gene="trmL"
                     /locus_tag="PLS64_02280"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HRL42694.1"
                     /translation="MATLNLVLVEPEIPQNTGNVARTCAATGTRLHLVKPMGFEPDDK
                     KLKRAGLDYWHLLDITYYENIDDFFEKTKGGKYFFFSTKGTHRHSDVEYPDNCYLLFG
                     KETKGLPEELLMQHPDDTLRLPMIDEARSLNLSNSVAIAAYEVLRQWDYPALQNKGEL
                     HNHKWSDLNNMT"
     gene            1857..3065
                     /locus_tag="PLS64_02285"
     CDS             1857..3065
                     /locus_tag="PLS64_02285"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRL42695.1"
                     /translation="MQLNKVSVDDINVKGKKVLVRCDFNVPLKDGVITNDNRITAALP
                     TIKKLIADGGKVILCSHLGKPKNGPEEKFSLAPVAVRLSELLGQDVVFANDDEVVGEN
                     AKKAVAEMKDGDVVLLQNTRFRKEETKNLEPFSSELASLADVFVMDAFGSAHRAHCST
                     VGVTDHIKDTAVGYLMQKEIDYLGNAVETPVRPFVAILGGAKVADKLNVISNLLEKCD
                     TLIIGGGMAYTFLKAQGKEVGLSLVDDTKIDYCKEMMAKAEKLGKKLLLPIDTTIAAG
                     FPDPIDAEIEVKVVDSDKIPADMEGLDIGTKTQELFADAVKSAKTVVWNGPMGVFENP
                     TLAKGTIAVAKALADTDATTIIGGGDSAAAVIQLGFADKMSHISTGGGASLEYLEGKV
                     LPGIAAIADK"
     gene            3159..3926
                     /gene="tpiA"
                     /locus_tag="PLS64_02290"
     CDS             3159..3926
                     /gene="tpiA"
                     /locus_tag="PLS64_02290"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRL42696.1"
                     /translation="MDKSKRKAIIAGNWKMNKTPKEAKELLNAITPLVKDAGCEVIAC
                     VPYVDLATALEATEGTNVKIGAENCHWAESGAFTGEISTGMLKEMGVEYVVLGHSERR
                     QYFGETDETVNKRTKAALAAGLKPIVCVGELLWERECDITEEVIARQIKLDLFNVTEE
                     ELKNVVIAYEPVWAIGTGKTATADQAEEVCSFIRATIAKLYNQAAADAVTIQYGGSMN
                     DGNADELLSKTNVDGGLIGGASLVAEKFAAIVKAASK"
     gene            4053..5564
                     /gene="gpmI"
                     /locus_tag="PLS64_02295"
     CDS             4053..5564
                     /gene="gpmI"
                     /locus_tag="PLS64_02295"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRL42697.1"
                     /translation="MKKPVILMILDGFGIAPEKGNAIKAAKKPNIDKLFASNPLTQIG
                     ASGMDVGLPDGQMGNSEVGHTNMGAGRIVYQELTRITKTINEDKLKDNEAIVDAMDKA
                     LKNGTALHLMGLLSDGGVHSHIEHLYGILELAKKKGLKDVYIHAFLDGRDVPPSSAAE
                     YADKLLDKLKEIGIGKVATVEGRYYAMDRDNNWDRVEKAYAAMVYGEGNKADCPVCAI
                     KNSYNDGVTDEFVVPCVIEGGAQVKPNDSIIFFNFRPDRAREITRTFVDPDFKGFERK
                     NGFFPVNFVCMTQYDATMPNVEVAFKPEVLKNTLGEYVSDKGMTQLRIAETEKYAHVT
                     FFFNGGVEKQYPGEDRILVKSPAVATYDLQPEMSAYEVTDKLVPAIKSGKYDMIILNY
                     ANCDMVGHTGVFEAAVKAVEAVDTCVGKVVDAIKEMGGVALITADHGNADKMVAEDGS
                     PFTAHTTNPVPFCVVNYDCELREGGRLADIAPTMLQIMGLEQPEEMDGTSLIK"
     gene            5759..7498
                     /locus_tag="PLS64_02300"
     CDS             5759..7498
                     /locus_tag="PLS64_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42698.1"
                     /translation="MIKLRRYLAYYKKECIIGPLCKLFEAILELLVPLVMAGIIDNGV
                     RNGDTAYIWRMGGLLVILATVGLCSALVCQYLASKASQGVGTRLRRDLFLHINKLSHA
                     ELDKLGTPSLITRITYDTNQVQQSVAMAIRLLTRAPFIVIGSMVMAMTINLEMSIIFF
                     IAAILIAVTLYLIMTKSIPIFEKMQKKLDKISLICRENLSGNRVIRAFSKQKNEKKRI
                     DDSTEDLAKTSIRVSILSALLSPVTYIITNAAIILIIWFGAQNVNAGNGMLSGDIIAL
                     VNYMTQILLAMIVVANLVVLLTKASASAARLNEVFETQPSVCESTTENIEISESESTP
                     KIEFDNVNFTYGTGDDELEDISFKIKRGQTVGLIGGTGCGKSTLVNLIPRFYDATQGT
                     VSVDGRNVKDYPFLQLRSQIGIVPQQSILFKGTIRDNMKWQNENATDEDIIKALKIAQ
                     AYEFVSKLNKGLDSFVEQGGKNFSGGQRQRLCIARALTGSPKLLILDDSFSALDFATD
                     AALRKALRENTKDMTVIIVTQRCSTIKNADLILVLDDGRLVGKGPHDELFESCETYRE
                     ICLSQLNEEEAKR"
     gene            7495..9243
                     /locus_tag="PLS64_02305"
     CDS             7495..9243
                     /locus_tag="PLS64_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42699.1"
                     /translation="MKNSAKKSSSVKKVLKKIKPYSFYLILALVSAIISVSLTLYIPV
                     LTGNAIDNIIDKGNVDFASVIQILVYIGVGIGGVALFQWIMTYFTNIVSYRTVRDLRR
                     EVFEKFNTVPLSYIDTTPHGDLISRVINDVDAVGDGLTQMFLQLFSGIVTIVGTLVFM
                     LTINWKIALVVVVLTPLSLFVAAFIGKMTHNRFTRQQALNGDISSYVEEHIGNQRIVK
                     AFSYEDRAFEEFEKYNDELLEVGFKAQFAGALANPSTRFVNALVYAAVGIMGALFAVA
                     GTLSVGQLSCFLTYANQYTKPFNEVTGVLTQLQTGIAAAGRVFEVLEADNEIPDNSTK
                     ELEHCEGNVKIENVNFSYTKEKPLITNFSLNVKSGSHIAIVGPTGCGKTTFINLLMRF
                     YDTDSGKISIDGVDIMDITRDNLRKCYGMVLQDSWLFNGTIMENLRYGNENATEEEVI
                     AAAKAAYAHSFIRRMSDGYNTVISEDGGNLSQGQKQLLCIARAMLTNPSILILDEATS
                     SIDTLTEIRVQKAFAKMMKGKTSFVVAHRLSTIKESDVILVMKDGNIIEQGTHEELLA
                     KGGFYNKLYNSQFAKQ"
     gene            complement(9413..10585)
                     /locus_tag="PLS64_02310"
     CDS             complement(9413..10585)
                     /locus_tag="PLS64_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRL42700.1"
                     /translation="MTKLLVKLFVKDSDNCDNPKVRTAYGNLSGVCGIILNLLLFGGK
                     LFAGIVSASVSVIADALNNLSDAGSSVVTFLGFKLASRPADREHPFGHGRYEYVAGLG
                     ISVVILLMGVELIKSSIEKIITPEDKTAFSTLSLAIMIASIFVKLWMYFFNSGLSRKI
                     NSTALKAAALDSLTDCIATSVVIIGLLISASTGVQIDGWLGVCVSLFILFTGVSTFRE
                     SLSPLLGKAPDAEFVDEIKETVLKNDMIVGIHDLIIHDYGPGRCFISLHAEVPCDEDI
                     LEAHDAIDLTEKQLENRYNCFATIHMDPIASNDAFTNELKMKVEEGLCELNPEYSIHD
                     FRIVKGSTHTNVVFDMVIPYGLDLTERQIKQSAIEKIKSIDEKLYPIINVEKSLSD"
     gene            complement(10609..11505)
                     /locus_tag="PLS64_02315"
     CDS             complement(10609..11505)
                     /locus_tag="PLS64_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRL42701.1"
                     /translation="MKNLLQRARKTIKIKNMLFLTIAGIINAFGITIFLNPVNLYDSG
                     ISGTAMLAAQLTPESLSLSLFLIIFNLPLFLFGLKKQGKKFTFYAIYTVAVYSFVSWL
                     ITDVLPVDVSMASPLAGTDLLLCAVFGGVISGIGSGLAIKFGGAMDGIEVMAVIFAKK
                     IGVSVGSFVMAYNIVLYVVCGFILQSWILPLYSIVTYFAALKTIDFIVEGFDRAKAAT
                     IIASVSRCDEICNALSEEFQSGVTVMQAQGGYSGKDKVMIYFVVNRFQIGKLKEIVRE
                     IDKKAYISITEVADVFSRNDSL"
     gene            complement(11638..13710)
                     /locus_tag="PLS64_02320"
     CDS             complement(11638..13710)
                     /locus_tag="PLS64_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRL42702.1"
                     /translation="MKQYDAKKILNIALAGHSGSGKTSVAESILYLSKMSERLGKVAD
                     GNTVLDFDSEEIKRQTSIMTAVAPIEWKNTKINLIDTPGLFDFAGGVAEGMRAADTAL
                     VVVSGKDGVCVGTEKAVEAATKAGLTKVFFVNGLCDESARFYRVFESLKATFGPSVCP
                     VVVPYIVDGQANTYVNLFDYKAYEYGTDGSVKQTALPDMGDRLEGLREAIKEAVAETS
                     EELLDKFIMGEEFTPEEIILGVSQGVKDGTICPVFCGDAHNTFAIDQLLNSLTWLAPS
                     AADKSGEIGVDLDGEPVDVSVNDGGAAAAIVFKTVADPFIGRLSYVKVVSGKVSSDAP
                     LINMRTGAQERITKVLSVSGKKQSDVPYIGAGDIGAIPKLASTKTGDTLCSPLRKVVL
                     DGIDYPSSSYTMAVYPAKKGDEDKVAQGIAKLADEDPTIKLVSNHETHEMLISGLGEQ
                     HLDVVISRLKTKYNVDAVLKKPKIAYRETIRKKVSAQGRYKKQSGGHGQFGDVWIEFE
                     PCDSDGLVFAERVVGGAVPKNFFPAVEKGLQDSIKKGVLAGYPMVGIKATLYDGSYHP
                     VDSSEMSFKTAASLAYKNGIPNASPTLLEPIGSLKANVPDANMGDVMGEVNKRRGRVL
                     GMQPGENGTQIVEAEVPMAEMDDFATYIRQVTQGRGSFEFAFVRYEDTPANVAQKIIE
                     KAKADSQE"
     gene            complement(14029..15687)
                     /gene="ilvD"
                     /locus_tag="PLS64_02325"
     CDS             complement(14029..15687)
                     /gene="ilvD"
                     /locus_tag="PLS64_02325"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRL42703.1"
                     /translation="MKSSVIKDGVSTAPQRSLLNALGLTEEEIKKPFIGIVSSKNDIV
                     PGHMNIDKIVDAVKQGVALAGGVPIVFPAIAVCDGIAMGHEGMKYSLVTRELIADSTE
                     AMAKAHAFDALVMVPNCDKNVPGMLMAAARINVPTVFVSGGPMLAGHVKGKKTSLSSM
                     FEAVGAYTANKITAEELAEFENKACPSCGSCSGMYTANSMNCLTEAIGMGLQGNGTIP
                     AVYSDRIKLAKHAGMKVMELLEKDIRPRDILTEKAFMNALTVDMALGCSTNTMLHLPA
                     IAKEAGVKLNLDIANEISKKTPNLCHLAPAGHTYMEDLNEAGGVYAVMNELTKVGLLD
                     TDLITATGKTVGENIKGCEIKDTDIIRPVTNPYSKTGGIAVLKGNLAPDGSVVKRSAV
                     AEEMMHHKGAARVFDCEEDALSAIYDGKINPGEVVVIRYEGPKGGPGMREMLNPTSAI
                     MGSGLGNCVALITDGRFSGATRGAAIGHVSPEAAVGGPIALVKEGDMIEIDIPANTIN
                     LLVSDEELAKRKAEWKPRQPKITTGYLARYAKLVTSGAQGAVLE"
BASE COUNT         4662 a         3594 c         3437 g         4306 t
ORIGIN      
        1 gccaccaaaa acttaataat attgacggag gtttttataa tggcaagaac aattattaac
       61 ctgccactgc gaaacagtgt tgatgaatca tacaacactg ctgtaaatat tttgcaggca
      121 aacggtttta aagaggtttc ctacaacggc gaaactgtat ggaaaaaagg tacaggactt
      181 ttgactgcta tgcaatacat aaaaattgaa tttacagata caacgctcgt tgtatctggt
      241 tgggtacaag caggtgtcgg tagtgttggc ggaaacgaaa tggcacttaa cggtattgtt
      301 gccgctgtac caaaaaaatc tgttatgaag gtcattgaac agattaaggc aagtctgtaa
      361 acagaggtaa caatatgata aaattaagaa aaatatcaat ggtgttatgt ataattacga
      421 tgttgtttac ggttatgtca ttaagcggtt gcagtaacaa cagcataacc ggaatatggg
      481 catttgttga cgacgatggg caagttagcg aaaattcaaa aatgtatttt aatgatgacg
      541 gttcttgcaa tgatattgat tatcatggtc cgacaagtgc tgaccctgtg tcatacaagg
      601 tcatgagtga tggaaaactt gtattcacaa tggaatggga cggcacatca tcatttgacc
      661 ttgctcaaag cgaagaggaa gccatgaatg attatcacaa atattatatt tcaggcgaca
      721 cattgatttt caggaaaagc acatacaaga gagtataaaa ttaccccccg attcgtttga
      781 gtcggggggt tgtttatgat gaatatgatt ttatctgaac atatactgtg cggtgtcgag
      841 gactgactcc attgtcttaa aggctttgtt tgcctttttc ttgagttttg ggttgctgtc
      901 taccatattt ttgccgacaa tacccatgca gacgcctgcc gtcattccta ttgcaacgcc
      961 tttcatcaca ttcatcattg gacttgtatt cataatcaca cctcccaatc ttgcaaattc
     1021 attgacaaaa tgtgtgtttt ttcatctttg tcacacggct ttgtcaaaaa aacttgcatt
     1081 attattgttt acgatttaaa atgtatttaa acagaaaaag aatggaataa taaaaattgg
     1141 agtgataact tttggcaact ctcaaccttg tgcttgttga acccgaaatt ccgcaaaaca
     1201 cgggcaatgt ggcacgaacc tgtgccgcaa cgggaacaag acttcatctt gtcaagccta
     1261 tgggctttga acccgacgat aaaaaactca aaagagccgg acttgactac tggcacttgc
     1321 ttgacatcac atattatgag aacattgacg atttttttga aaaaacaaaa ggcggaaaat
     1381 actttttctt ttcaacaaaa ggaactcacc gccacagcga tgttgaatat cccgacaact
     1441 gctatcttct ttttggcaaa gaaacaaaag gcttgcccga ggagctttta atgcagcatc
     1501 ccgatgatac tttaagactg cctatgattg acgaggcaag aagtcttaat ctttcaaact
     1561 cggttgcaat tgcggcttat gaagttttaa ggcaatggga ttatcctgct ttgcagaaca
     1621 agggcgagct tcacaaccac aagtggagcg acctcaacaa tatgacataa gaataaaaat
     1681 aagtctctta aacttcctta aaatcagtat ttgtaaaggc agtgcaaaaa atacctttgc
     1741 aggcgaaatt tttaaagttt tctattgata aaaaggggtt tttgatatat aataatggtg
     1801 actgtaaaaa tactaaattt ttacaaattt cttcttacac gaaaggaatg tatattatgc
     1861 agctcaacaa agtatctgtt gatgacatca atgtaaaagg taaaaaagta cttgttcgtt
     1921 gtgacttcaa tgtaccgctc aaggacggcg taatcacaaa cgacaacaga attacagcag
     1981 ctcttcctac aatcaagaag cttatcgctg acggcggcaa ggttatcctt tgctcacatc
     2041 tcggcaagcc gaagaacggt cccgaagaaa agttctcact cgctcccgta gcagtaagac
     2101 tttctgaact cctcggtcag gatgttgttt tcgctaacga tgacgaggtt gtaggcgaaa
     2161 atgctaaaaa ggctgttgca gaaatgaaag acggcgatgt tgttcttctc cagaacactc
     2221 gtttcagaaa agaagaaaca aagaatcttg agccattctc atcagaactt gcttcactcg
     2281 ctgatgtatt cgtaatggat gcattcggtt cggctcaccg tgcacactgc tcaacagtag
     2341 gtgttacaga ccacatcaag gacacagcag taggttacct catgcagaag gaaattgact
     2401 acctcggcaa tgccgttgaa acacctgttc gtccgttcgt tgcaatcctc ggcggtgcta
     2461 aggttgctga caagctcaat gttatttcaa acctcctcga aaagtgcgac acactcatca
     2521 tcggcggcgg tatggcttac acattcctca aggctcaggg caaggaagtc ggtctttcac
     2581 tcgttgacga tacaaagatt gactactgta aggaaatgat ggctaaggct gaaaagctcg
     2641 gcaagaagct ccttctccca atcgacacaa caatcgctgc aggcttccct gatcctatcg
     2701 acgctgaaat cgaagttaag gttgttgatt cagacaagat tcctgctgac atggaaggtc
     2761 ttgacatcgg cacaaagaca caggaactct ttgccgacgc tgtaaagagc gcaaagactg
     2821 ttgtttggaa cggtcctatg ggcgtatttg agaacccgac tcttgcaaag ggtacaattg
     2881 ctgttgctaa ggctcttgcc gacacagacg caacaacaat catcggcggc ggcgacagcg
     2941 cagctgctgt tattcagctt ggttttgctg acaagatgag ccacatctca acaggcggcg
     3001 gcgcatctct tgaatacctc gagggcaagg ttcttccggg tatcgcagca atcgctgaca
     3061 aataatttaa tcaaataaaa gggcggattt ttccgccctt actgatttaa ccctgttact
     3121 ttattataat aaaaacatat tctgaaagga aaattatcat ggataagtca aagagaaagg
     3181 caattatcgc cggcaactgg aaaatgaaca aaactccaaa ggaagctaaa gagctcctca
     3241 acgctattac tcccctcgta aaagatgcag gttgtgaagt aatcgcttgt gttccttatg
     3301 ttgaccttgc aacagcactt gaggctacag aaggcacaaa tgttaaaatc ggtgcagaaa
     3361 actgtcactg ggctgaaagc ggtgctttca ctggcgaaat ctcaacaggt atgctcaagg
     3421 aaatgggcgt tgaatatgta gttctcggtc acagcgagag aagacagtat ttcggcgaaa
     3481 cagacgaaac tgtaaacaag agaacaaagg ctgctcttgc cgcaggtctt aagccaatcg
     3541 tttgtgttgg cgaactcctc tgggagcgtg agtgcgacat cacagaagaa gttatcgcaa
     3601 gacagattaa gcttgacctc ttcaatgtaa cagaagaaga actcaagaat gttgttatcg
     3661 cttatgagcc tgtatgggct atcggcacag gcaaaactgc tacagctgat caggctgaag
     3721 aagtttgctc tttcatccgt gctacaatcg ctaagcttta caatcaggct gctgctgacg
     3781 ctgttacaat tcagtacggc ggttcaatga atgacggcaa tgctgacgag cttctctcaa
     3841 agacaaatgt tgacggtggt cttatcggcg gtgcatcact cgttgccgag aagtttgccg
     3901 caatcgtaaa ggctgcaagc aagtaattta aacagtatat tcaaacacaa tgccgtgtcc
     3961 ttttgggcac ggcacaaaag tctgtaatcg gcttttggat tttgtttttt gcataacaag
     4021 attgtgtatg ctttatggaa aggacacgca aaatgaaaaa accggtaatt ttaatgattc
     4081 ttgacggctt tggtattgct cccgaaaaag gcaacgctat caaggctgct aaaaagccaa
     4141 acattgacaa gctttttgca tctaatccgc tcacacagat tggtgcgtcg ggtatggatg
     4201 taggtcttcc tgacggtcag atgggtaact ctgaggtcgg tcacacaaat atgggtgccg
     4261 gcagaatcgt ttatcaggaa ctcacaagaa ttacaaagac tatcaacgag gacaagctca
     4321 aagataacga ggctattgtt gatgcaatgg acaaggctct caagaacggc acggcacttc
     4381 accttatggg acttctctca gacggcggtg ttcacagcca cattgagcat ctctacggta
     4441 ttcttgaact tgctaagaaa aagggtctta aagatgttta cattcacgca ttccttgacg
     4501 gccgtgatgt tccgccttca agtgccgctg aatatgctga caagcttctt gacaagctca
     4561 aagaaatcgg cataggcaag gttgccacag ttgagggcag atactacgca atggacagag
     4621 ataacaactg ggacagagtt gaaaaggcat atgccgctat ggtatatggc gagggcaaca
     4681 aggcagactg ccctgtttgc gcaattaaga actcttacaa tgacggcgta acagacgaat
     4741 ttgttgttcc ttgtgttatt gagggcggtg ctcaggttaa gcctaacgac agcatcatct
     4801 tcttcaactt ccgtcctgac agagcaagag agattacaag aacatttgtt gaccctgatt
     4861 ttaagggctt tgagagaaag aacggtttct tccccgtaaa ctttgtttgc atgactcagt
     4921 atgatgcaac aatgcctaat gttgaagttg cattcaagcc agaggttctc aagaacacac
     4981 tcggcgagta tgtatccgac aagggtatga cacagctcag aattgcagaa acagaaaagt
     5041 atgctcatgt aacattcttc ttcaacggcg gtgttgaaaa gcagtatccg ggtgaggaca
     5101 gaatccttgt taagtcacct gctgttgcaa catatgacct tcagcctgaa atgagtgctt
     5161 acgaggttac agacaagctc gttcccgcaa tcaagagcgg taagtacgat atgattattc
     5221 tcaactacgc taactgcgat atggttggtc acacaggcgt attcgaggca gccgtaaagg
     5281 ctgttgaggc tgttgacacc tgcgttggca aggttgttga cgcaatcaaa gaaatgggcg
     5341 gtgttgctct tatcacagcc gaccacggca atgcagataa gatggttgca gaggacggtt
     5401 caccgtttac tgctcacaca acaaaccctg ttcctttctg cgtagtaaac tacgactgcg
     5461 agcttcgtga gggcggcaga cttgccgata tcgctccgac aatgctccaa attatgggac
     5521 tcgagcagcc cgaagaaatg gacggcacaa gccttattaa gtaatttatt ttactgatta
     5581 tatacagagc caccgatgtt ttatcggtgg cttttttgtg ttagcacaaa actgtagggg
     5641 tagccttgcg accgccgcaa attcccgcaa cgcaaaattt aagttgtaaa atatcgttaa
     5701 tcggtgtaaa ataatattac ttatgtattg ttaattatat ggcagagaag gggaaaattt
     5761 gattaagcta cgaagatatc ttgcatacta caaaaaggaa tgtatcatcg gtccgctttg
     5821 caagctgttt gaggcaattt tggagttgct cgttccgctt gttatggcag gaatcatcga
     5881 caacggtgtg cgaaacggcg acacggcata catttggaga atgggcggtc tgctcgttat
     5941 tcttgcaact gtaggacttt gcagtgcact tgtgtgtcag tatcttgcat caaaggcatc
     6001 tcagggtgtg ggaacaagac ttcgccgaga tttgtttttg cacatcaaca aactctctca
     6061 cgcagagttg gacaagctcg gcactccgtc acttataaca cgaatcacat atgacaccaa
     6121 tcaggttcag caatctgttg caatggctat ccgcctgctc accagagcac cgtttattgt
     6181 tatcggttca atggttatgg caatgacaat caaccttgaa atgtcaatta ttttcttcat
     6241 tgcggctata ttgattgccg ttactcttta cctcattatg acaaagagta ttcctatttt
     6301 tgaaaaaatg cagaaaaagc tcgacaaaat cagccttatc tgccgtgaaa atctttcggg
     6361 caacagggtt atcagagcct tttcaaagca gaaaaatgag aaaaagcgta ttgacgattc
     6421 aaccgaggat ttggctaaaa cttcgatcag agtaagcatt ctttcggcac ttttaagccc
     6481 tgtcacctac atcatcacca atgctgcaat tattctcatc atttggtttg gcgctcagaa
     6541 tgtaaacgca ggcaacggaa tgctttcggg tgacatcatt gcccttgtaa actatatgac
     6601 gcagattttg cttgcaatga ttgttgttgc aaaccttgtt gtacttctca cgaaggcaag
     6661 tgcaagtgcg gcaagactta acgaagtgtt tgaaactcag ccgagtgtgt gtgaaagcac
     6721 taccgagaac attgaaattt cagagagcga aagcactccg aaaatcgaat ttgacaatgt
     6781 aaacttcaca tacggcacgg gtgatgatga gcttgaggac atcagcttta aaatcaagcg
     6841 tggtcagacg gtcggtctta tcggcggtac gggttgcggt aaaagtacgc ttgtcaacct
     6901 tatcccccgt ttttacgatg caacacaggg tactgtaagc gttgacggca gaaatgtcaa
     6961 ggactatccg ttcttacagc tccgttcaca gattggtatt gttcctcagc agtcaattct
     7021 tttcaaggga acaatccgtg acaatatgaa atggcaaaac gaaaatgcaa ctgacgagga
     7081 tattataaag gctctaaaaa ttgcacaggc atatgagttt gtaagcaagc tgaacaaagg
     7141 gcttgacagc tttgttgaac agggcggaaa gaacttcagc ggaggtcagc gtcagcgact
     7201 ttgcattgcc agagcactca caggctcacc gaagcttctc attcttgacg acagcttttc
     7261 ggctcttgac tttgcaacag atgcggcact cagaaaggct ttgcgtgaaa acacaaagga
     7321 tatgaccgtg ataatcgtta cacagcgttg ctcaacaatc aagaacgcag accttattct
     7381 tgtgcttgat gacggcagac ttgtaggcaa aggtccgcac gatgaactgt ttgaaagctg
     7441 cgaaacctac cgtgaaatct gtctttcaca gcttaatgaa gaggaggcaa aaagatgaaa
     7501 aattcagcaa aaaaatcctc ctctgtaaaa aaggtactca aaaaaataaa gccgtattcg
     7561 ttttatctga ttttagctct tgtgagcgca atcatcagcg tttcgctcac gctctatatt
     7621 cctgttctca caggtaacgc aatcgacaac atcattgaca agggcaatgt tgactttgca
     7681 agcgttattc agattcttgt gtacatcggt gtaggcatcg gcggtgtggc acttttccag
     7741 tggattatga cctactttac caacattgtc agctaccgca ctgttcgtga cctcagaaga
     7801 gaggtttttg aaaaattcaa cacagttccc ctctcctaca ttgacacaac acctcacggc
     7861 gaccttatca gccgagtaat caacgatgtt gacgcagttg gtgacggtct tacacagatg
     7921 tttctacagc ttttttcggg cattgttacc attgtcggca cgcttgtatt tatgctgaca
     7981 atcaactgga aaatcgccct tgtggttgtt gttctcacac cgctttcgct ctttgttgcg
     8041 gcattcatcg gcaagatgac acacaaccgc tttacccgtc aacaggcact caacggtgac
     8101 atctcgtcat atgttgagga gcatatcggc aatcagagaa ttgtaaaggc tttttcatat
     8161 gaggacagag cctttgaaga atttgaaaag tacaacgatg aacttcttga agtgggattc
     8221 aaggcgcagt ttgcaggcgc acttgccaac ccgtcaacaa gattcgtaaa cgcattggtt
     8281 tatgccgcag taggcattat gggtgctctg tttgccgttg caggcacact ttcggtcgga
     8341 cagttatcat gcttccttac ctacgcaaat cagtacacaa agccgtttaa cgaggttaca
     8401 ggcgtactca cgcagttgca gacaggcatt gcggcggcag gacgggtttt tgaggttctt
     8461 gaggcagata acgaaattcc cgacaacagt acaaaagagc ttgagcattg cgaaggcaat
     8521 gtcaagattg agaatgtaaa cttctcctac acaaaagaaa aaccgctcat cacaaacttt
     8581 tcgcttaatg tcaagagcgg cagtcacatt gccattgtcg gtccgacagg ttgcggcaag
     8641 acaacattca tcaatcttct tatgagattt tatgacacgg acagtggcaa aatcagcatt
     8701 gacggagttg acattatgga tatcacccgt gacaacctca gaaaatgcta cggaatggtt
     8761 cttcaggaca gctggctgtt caacggaacg attatggaaa atctgcgtta cggcaacgaa
     8821 aacgcaaccg aagaagaagt tattgccgct gcaaaggcag catatgcgca ctcattcatc
     8881 cgccgtatgt ctgacggcta caacaccgta atcagcgagg acggcggtaa tctcagtcag
     8941 ggacagaaac agcttttgtg tattgcaagg gcgatgctta caaacccgtc aatccttatt
     9001 cttgacgagg cgacttcatc aattgatacc cttaccgaaa tccgtgttca aaaggctttt
     9061 gccaagatga tgaagggcaa gacgagcttt gttgttgccc acagactttc aacaatcaag
     9121 gaaagcgatg ttatccttgt tatgaaggac ggcaacatca ttgaacaagg cacacacgag
     9181 gagttgctcg ccaaaggcgg attctataac aaactttaca acagtcagtt tgcaaaacaa
     9241 taatacggct acggtcagta accgccaatc gaaacaattg taaaatcgtt tcgacaataa
     9301 accgaataac tgactactac actcgaacac acaattttta acaaatccga aaatatatac
     9361 aaaacgaggt catcagctaa aaaccgatga cctcgttttg tattatgaac ttttaatcag
     9421 aaagggattt ttcaacattt attatcggat aaagcttttc gtcaattgac ttgattttct
     9481 cgattgcact ctgtttaatc tgtctttctg taaggtcaag tccatacgga ataaccatat
     9541 caaagacaac atttgtatgc gtactgccct ttacaattct gaaatcgtgg attgaatatt
     9601 cgggattaag ctcacacaag ccctcctcca ccttcatttt aagttcattt gtgaacgcat
     9661 cgtttgacgc aatcggatcc atatgtatgg ttgcaaaaca gttgtacctg ttttcaagct
     9721 gtttttccgt caggtcaatt gcgtcgtgag cttcaaggat atcctcgtca cacggtacct
     9781 ctgcgtggag tgaaataaag catctgcccg gaccgtaatc gtgaataatc aaatcgtgga
     9841 tacctacaat catatcattt ttcaacactg tttccttgat ttcatcaaca aattcggcat
     9901 ctggagcttt gccgaggagc ggagagaggc tctctctgaa tgtgctgaca cccgtaaaaa
     9961 gtatgaacag cgaaacacac acgccgagcc aaccgtcaat ctgaactcct gttgaggcag
    10021 aaataagcaa tccgattatt acaaccgagg ttgcaatgca gtcggtcaaa gaatcgagtg
    10081 ccgccgcctt gagtgcggtt gaatttatct ttctcgacaa tcccgaatta aaaaagtaca
    10141 tccataattt tacgaaaatt gacgcaatca ttattgcgag tgaaagcgtt gaaaatgccg
    10201 ttttatcctc gggagtaatt atcttttcta tcgaactttt tataagctca acacccatta
    10261 aaaggattac aacggaaatt cccaagcctg ccacatactc ataacgaccg tgaccgaacg
    10321 gatgctctct gtcggcagga cggcttgcaa gcttaaatcc gaggaatgta acaaccgaac
    10381 tgcctgcgtc cgaaaggttg ttgagggcgt cggcaatgac ggaaaccgat gccgaaacaa
    10441 ttccggcaaa caattttcct ccgaacagta acaggttaag aattattccg caaacacccg
    10501 aaaggttgcc ataggcagtt ctaacctttg gattatcgca attgtcgctg tctttaacga
    10561 acagctttac aagtaatttt gtcaaataaa acaccaacta aaatttattt acagcgaatc
    10621 gtttcggctg aaaacatcgg caacctcagt aattgaaatg tacgctttct tgtcgatttc
    10681 acgcacaatt tccttcagct tgccaatctg gaaacggttg acaacaaagt aaatcatcac
    10741 cttgtccttg cctgaatatc cgccctgagc ctgcattacg gtaacgccgc tttgaaattc
    10801 ctccgaaagt gcgttacaaa tttcatcgca acggcttaca gacgcaataa tcgttgccgc
    10861 ctttgccctg tcaaaacctt caacaataaa gtcaatcgtt ttaagtgcgg caaaatatgt
    10921 aacaatcgaa taaagcggaa gaatccagct ttgcagaatg aaaccgcata caacataaag
    10981 tacgatattg tatgccataa cgaatgaacc aaccgataca ccgattttct tcgcaaagat
    11041 tactgccata acctcgatgc cgtccattgc accgccaaat ttgattgcaa gaccgctgcc
    11101 tatacccgaa atcacaccgc cgaacacggc acaaaggagc aaatcggttc ccgcaagcgg
    11161 agacgccata ctgacatcaa cgggcagaac atcggttatc agccatgaaa cgaacgaata
    11221 aactgcaacc gtataaattg cgtaaaatgt aaacttcttt ccctgctttt taagtccgaa
    11281 caaaaacagc ggcaggttaa aaattataag aaaaagtgaa agcgacaggc tttcgggtgt
    11341 aagttgtgcg gcaagcatag ccgtacctga aattccgctg tcgtaaaggt tcacgggatt
    11401 aagaaatatt gtaattccaa acgcattgat aattccggca attgtgagaa aaagcatatt
    11461 tttgattttg attgtctttc tcgctctttg taaaagattt ttcattgtca tcatcctttg
    11521 caataaattt ccatccccat cattcacata agtatagcct aattgtatca caataatgat
    11581 atttaggcaa gaaaagcaaa aggcaccgaa aaatcggtgc cttaatcagc ttattgatta
    11641 ttcctgagag tcagccttgg ctttctcaat aattttttga gcaacatttg caggagtatc
    11701 ctcatagcgg acaaacgcaa attcaaatga tcctctgccc tgtgttacct gacgaatgta
    11761 ggttgcaaag tcatccattt ctgccatagg aacttccgcc tcaacaatct gcgttccgtt
    11821 ctcacccggc tgcataccga gtacacgacc tctgcgcttg ttgacctcgc ccattacatc
    11881 acccatattt gcatccggaa cattggcttt aaggcttccg attggctcaa gaagcgtggg
    11941 agaagcattc ggaataccgt tcttataagc cagagaagcc gctgtcttga atgacatctc
    12001 ggaggaatca acgggatgat atgaaccgtc atagagagta gccttgatgc ctaccatcgg
    12061 atagcctgca agaacaccct tcttgattga gtcctgaagt cccttttcaa cagcagggaa
    12121 gaaatttttc ggaactgcac cgccgacaac tctttcggca aatacaagac cgtccgaatc
    12181 acacggctca aattcaatcc acacatcacc gaactgaccg tgaccgcctg actgcttttt
    12241 atatctgccc tgtgcggaaa cctttttgcg gatagtttct ctgtatgcaa tctttggctt
    12301 tttgaggact gcgtcaacat tatactttgt tttaagtctt gaaattacaa catcaagatg
    12361 ctgttcaccg agtcccgaaa ttaacatttc atgggtttcg tggtttgaaa cgagcttgat
    12421 tgtcggatct tcgtctgcaa gctttgcaat gccctgagca accttatcct cgtcaccctt
    12481 ctttgcggga tacacagcca ttgtatatga tgatgacgga tagtcgattc cgtcaagaac
    12541 aacctttctg agaggcgaac agagtgtgtc gcctgtcttt gttgatgcaa gcttcgggat
    12601 tgcaccgatg tcgcccgcac cgatgtacgg aacatccgac tgctttttac cgcttacgga
    12661 aagcaccttt gtaattctct cctgtgcgcc tgtacgcata ttgatgagag gagcgtccga
    12721 gcttaccttg cccgaaacaa cctttacata tgaaagtctg ccgatgaacg ggtctgcaac
    12781 cgtcttgaat acgattgcgg cagccgcacc gccgtcatta accgaaacat caacgggttc
    12841 accgtcaagg tcaacaccga tttcaccgct cttgtcagcc gctgacggag caagccatgt
    12901 aaggctgttc aaaagctgat caattgcgaa tgtattatga gcgtcaccgc agaatacggg
    12961 gcagattgtg ccgtccttaa cgccctgact tacaccgaga atgatttctt cgggagtaaa
    13021 ctcctcaccc ataataaact tgtcaagaag ttcctcgctt gtttcggcaa cagcctcctt
    13081 gatagcctca cgcaagccct caagcctgtc gcccatatca ggaagcgcag tctgttttac
    13141 actgccgtct gtgccgtact cataagcctt gtaatcaaac aaattaacat aagtgtttgc
    13201 ctgaccgtca acaatgtacg gaacaacaac aggacaaaca gacggaccga atgtagcctt
    13261 caagctctcg aatacacgat agaaacgggc actttcgtcg caaagaccgt ttacaaagaa
    13321 cacctttgta agccctgcct ttgttgccgc ctcaactgct ttttcggtac ctacacagac
    13381 accgtccttg cccgaaacaa cgacgagcgc tgtgtcagcc gctctcatgc cctctgcaac
    13441 accgcctgca aaatcaaaaa gaccgggggt gtcgattagg ttgatttttg tatttttcca
    13501 ttcaatcggt gcaaccgccg tcataattga agtctgtctt ttgatctcct cgctgtcaaa
    13561 atcaaggact gtgttgccgt ctgcaacctt accgagtctt tcgctcatct ttgagagata
    13621 gagtattgac tccgcaaccg aggttttgcc gctgccgctg tgtcctgcga gggcaatatt
    13681 aagaattttt ttagcgtcgt attgtttcaa aagtgaaatc ccctttcgta aaatttctat
    13741 tctaatcaag cttatttcga caaaattaaa caatagtgtc aattaatacg gaattattat
    13801 agcatatttg tataaaataa acaaccgaat tatggcaaaa taaaaaaatt caccctttgc
    13861 ccaaatggac aagggatgaa ttgtgcaact tgctttttta ttttatgtta agctgaaaaa
    13921 tatacagatt tttgcaaggt caattgtgca ttttaacgat tgggctttct gaaaaaacaa
    13981 ttttcgacaa aaggaaaggc tctcaaaacg agagccttga aaacttgatt attcgagaac
    14041 cgcaccctgt gcgcctgatg taacaagctt tgcataacga gcaaggtagc ctgttgtaat
    14101 cttgggctga cggggcttcc attctgcctt acgctttgca agctcttcgt cagaaacgag
    14161 gagattgatt gtgtttgcag gaatgtcgat ttcaatcata tcgccctctt taacaagtgc
    14221 aatcggaccg ccgactgctg cctcgggaga tacatgaccg atagccgcac ctcttgttgc
    14281 gcctgagaaa cgaccgtctg tgataagagc aacacagttg ccgagaccgc tgcccataat
    14341 agcggaagtc gggttgagca tttctctcat acccggaccg cccttaggac cttcgtatct
    14401 gataacaaca acctcaccgg gattgatttt gccgtcataa attgcactga gagcgtcctc
    14461 ttcgcagtca aacactcttg ccgcaccctt gtggtgcatc atttcctctg caacggcact
    14521 tctcttaaca acagaaccgt caggagcaag gttacccttg agaacagcaa taccgcctgt
    14581 cttactgtat ggatttgtaa cagggcgaat gatgtctgtg tccttgattt cacaaccctt
    14641 aatgttctcg cctacagtct tgcctgttgc cgtgataagg tctgtgtcaa gaagtccaac
    14701 ctttgtaagc tcgttcatta cggcataaac accgcctgcc tcgttgagat cctccatata
    14761 tgtgtgacct gcaggagcaa ggtgacaaag gttaggagtc tttttgctga tttcattggc
    14821 aatgtcaagg ttaagcttaa cacctgcctc ttttgcgatt gcaggaaggt ggagcattgt
    14881 atttgttgaa cagccgagag ccatatcaac agtaagagca ttcataaatg ctttttccgt
    14941 gagaatgtca cgaggacgga tatccttttc aaggagttcc ataaccttca tgccggcgtg
    15001 ctttgcaagc ttgattctgt ctgagtaaac agcaggaatt gtgccgttac cctgaagtcc
    15061 cataccgatt gcctcggtaa gacagttcat actgttggcg gtgtacatac ccgaacatga
    15121 accgcatgac ggacaagcct tgttttcaaa ctcggcaagc tcttcggcag taatcttgtt
    15181 tgccgtgtaa gcgccgactg cctcaaacat actcgaaaga cttgtctttt tgcccttaac
    15241 atgacctgca agcataggac cgccgcttac aaaaactgtc ggaacattaa ttcttgcggc
    15301 agccataagc atacccggaa cattcttgtc gcagttagga accattacaa gtgcgtcaaa
    15361 cgcatgagcc tttgccatag cctctgttga gtctgcgata agctcacggg ttacgagaga
    15421 atacttcatt ccctcatgtc ccatagcaat accgtcacaa acagcgattg cagggaatac
    15481 aatcggaaca ccgcctgcaa gagcaacgcc ctgcttaacg gcgtcaacaa tcttatctat
    15541 attcatatgt cccggaacga tatcgttctt ggaacttaca ataccgataa aaggtttctt
    15601 aatttcttct tcggtaagac cgagagcatt caaaagcgaa cgctgaggtg cggtacttac
    15661 gccgtccttt ataactgaac ttttcattgt aaacaccctt tccataatta tcaaatcgga
    15721 acaaaattca cataaagccc ttcaattgtt ccataataaa acacagtgaa aatttctgta
    15781 tcgttaatct taattttagc acatcggctg ttcaaaatca agtagagaac ctatattgcc
    15841 aaaatcattt attgcggctc aatcgttgat tattctgccc ttttcaacat tgggcataat
    15901 aaagttatcc cttgaatatt cccacagctt ttttgagccg tcggcagtat ttttgttgcg
    15961 gttaaggata ctgtgcaggt gaacaatatg ctctaccca
//
LOCUS       DAOMVE010000022        15999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_741_length_15999_cov_3.012356, whole genome shotgun sequence.
ACCESSION   DAOMVE010000022 DAOMVE010000000
VERSION     DAOMVE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15999
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_741_length_15999_cov_3.012356"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..773
                     /locus_tag="PLS64_02330"
     CDS             285..773
                     /locus_tag="PLS64_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HRL42704.1"
                     /translation="MYSIGEIVLYGSNGVCEITEITTKRIGKDSIEYYVLKPVCSDSS
                     TLFVPTQNEMLVSRMRVVLSSDEIKDILSQKTDNEIWIDNKAERCEKIKEIISGGDCM
                     KLVELIRRMHFHSKLQLKKGRRLHITDERFLKEAEKMVCDEVSVVLHIDRNDVLPLVL
                     AG"
     gene            945..2972
                     /locus_tag="PLS64_02335"
     CDS             945..2972
                     /locus_tag="PLS64_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRL42705.1"
                     /translation="MRNFKKTLSFLLSTVIIVSSVAGVGTTVFAATDEHTYKNFKYRI
                     LDDNTAEICGYTGKNAVVTVPDSIDSKPVTSIGRTAFYSGKIKEVTIPNTVRSIKWWA
                     FYGCESLEKINLNFGLKTVGYGAFMNCKNLKSVSIPMSVTQICDDSFAVSCSTKKGVF
                     DTYSKQTISTQQYSTDSSFTLEGYSGTVAEKYCNDNSLNFVSSGNVIYGDVNNNGTVE
                     AADAKLAKSLIDTVPTEEELTAADVDDDGKITENDVNLILQAVGNEFYAQLFPCAKAM
                     YSAPDYLSGRTMYCDGDSVAYGSGTDTMGNSTYSFCNYVAEKYNMTMTNKATPGTTLA
                     IRKDFIGTDHRSILERVREMKGSYDVILLDGGFNDLFKNVEMGAMTDINNKSGKYNEY
                     TTAGALESICYFLDKNYKDSIKLFVLCHNCSTRTKQSQYWSLMKNILDKWEIPYVDLS
                     EETELTGDNEEITTQYFRYNATTKKGDGIHPLAYANMKIYGPIVAEKLNETVQSKSEL
                     VLPKSDISMGLFESYTLNSEITELRGDIDVSYSSSNPSVASVDENGNIVATGIGDTVI
                     TISTSDGKTKNVNVNVKFLAMAVSFGKNKISLSEGNSSLLNLSVADGEATCSTTYSST
                     DPTVASVDENSGKITANKTGKTTVSCTTANGVTVRCLVYVTSSALTKMQKA"
     gene            2980..4215
                     /locus_tag="PLS64_02340"
     CDS             2980..4215
                     /locus_tag="PLS64_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRL42706.1"
                     /translation="MKIFNRKLKITSSALLTLCMVFVMTACAENSSQSEKSQPAEQTT
                     VQPTTMSAEEINDRKLDKFIADMTLEEKVGQMFFVRCPDEDAVQQVSEYNIGGYILFG
                     RDFDGKTKDEVVDDIHSYQNEADIPLLIGVDEEGGTVVRVSSNPNLRETPFLSPKDTY
                     ADGGWDAVKHDAEEKANLLLSLGINVNLAPVCDMTSDEYGFMYDRSFSSDVDMENRYV
                     RTVVETSKSKKLGTVLKHFPGYGNNSDTHTGIAYDDRDYSEFENTDFKPFYQGIESGA
                     DCILVSHNIVNCMDSEYPASLSQKVHDILRNEFKFDGVIMTDDLIMDAITDFTGDEAA
                     AVTAAKCGNDLLCCSSVDTQYPAVLEAVQNGEIPEAQVDASVKRILKWKQNLGIFNID
                     TYQKKVKSTEPTDTVGSEE"
     gene            complement(4677..5138)
                     /locus_tag="PLS64_02345"
     CDS             complement(4677..5138)
                     /locus_tag="PLS64_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HRL42707.1"
                     /translation="MTVLVDADACPVVKSVERIAKKHNVPVVLLCDTNHVLKSDYSEI
                     KIIGAGADAVDLALVNLCKKGDIAVTQDYGVAALVLGKGAYCIHQSGKIFSDDNIGGL
                     LMDRHLAKKARMSNGKHHIKGPKKRTKQDDENFEKSFESLLVKVFNNSNQE"
     gene            complement(5147..5818)
                     /locus_tag="PLS64_02350"
     CDS             complement(5147..5818)
                     /locus_tag="PLS64_02350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL42708.1"
                     /translation="MTKIKKISAIMVAAVILCTCFLMSGCGGDTESSSSEKATSAVSN
                     EETTSASTDTTDYKSSYTATIDVKDYGKIEVELYEELAPITVKNFVDLAKDGFYDGLT
                     FHRIITGFMIQGGDPKGDGTGGSEKTIKGEFAQNGVENNLSHTRGVISMARSNEPDSA
                     SSQFFIVQADSTYLDGQYAAFGKVTKGMDIVDKICEDTPVTDSNGTVEKKNQPVINSI
                     KIEEK"
     gene            5953..6795
                     /locus_tag="PLS64_02355"
     CDS             5953..6795
                     /locus_tag="PLS64_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL42709.1"
                     /translation="MKNNEITDITSKRRLFELNLGDVRKYRDLVMLFVKRDFKNSYKQ
                     TVLGPLWIIIKPFLSTFVFTVIFGMIANISTDGMPQFLFFMAGNILWSFFSSCLNGSS
                     STFIGNARLFGKVYFPRLVMPCANIIYNTIGFLINFVMFFILVLIYMFTGSNVHPNLC
                     ALLMPLILLSSALLGTGCGLIVSSVTTKYRDLGVLVSFGVTLWMYITPVVYPVSQVSD
                     SFRPLALLNPVAPLIETFRYGFLGSGTFPWQYLILSFAVTAVVLLFGIIVFNRVEKNF
                     IDTV"
     gene            6800..8074
                     /locus_tag="PLS64_02360"
     CDS             6800..8074
                     /locus_tag="PLS64_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42710.1"
                     /translation="MTEMNENCVIKIENLSKEYRLGAIGSGTLRHDLQSWYAKKRGKE
                     DPNQRIGAKQYTKGDTFLALDGVNLEVNRGERIGIIGANGAGKSTLLKVLCRITSPTD
                     GRVRFRGRIASMLEVGTGFHAELTGRENIYLNGAILGMTKKEVTSKIDSIIEFSECEK
                     FIDTPVKRYSSGMFVKLAFAVAAHLDAEIMIMDEVLAVGDMHFQKKCIGKMRDLATEE
                     DRTVLYVSHNMNTIRDLCDRCIVLDHGKIVFDGDVEDAVKIYLGSANENFRAIEYVGN
                     EHKHHADRNDLCLQFAGYNGKEDNIFYDDEQILLELTWKNKADIENLCLRVEVSDYRR
                     YPVTAFVVENFYSGKKGETHTAKLKIDISKIVRGGYYTRYVFYSRKNDAAPFETLEVA
                     DGLTFRRRKPDKYQNTWQKAWGSIEMNDIEVV"
     gene            complement(8274..8816)
                     /locus_tag="PLS64_02365"
     CDS             complement(8274..8816)
                     /locus_tag="PLS64_02365"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRL42711.1"
                     /translation="MPSVYLSPSLQEYNPYIDGGNEEYYMNLIADAMEPYLRASGIDF
                     RRNDPDMTLSQAIADSNKGNHDLHLAIHSNAAPEGSAGRYTGADIYYYPTGSNGKRFA
                     EILQKNYKDIYPEPNDVDIIPTTSLAEVRRTKSPSALIEVAYHDNPEEAQWIRDNINA
                     IGRNLAKSVAEYFNMPFVEP"
     gene            9099..9761
                     /locus_tag="PLS64_02370"
     CDS             9099..9761
                     /locus_tag="PLS64_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRL42712.1"
                     /translation="MTDEMRAKVEKVMKNLERNKMKPYFCENREEAQALVKTLIKKGE
                     TISCGGSKTLDETGIYQIINSPDYNFLDRSAPGMTRPEVEEVYRQTFRADTFFMSTNA
                     LTENGELYNVDGNSNRVAALLYGPKSVIVVCGINKIVKNIDEAIKRVKTIAAPQNTIR
                     LGIETPCGKTGECVSLRKENPELCDGCHGDTRICCNYVVSAQQRHIDRIKVIIIGEEY
                     GY"
     gene            complement(9821..9952)
                     /locus_tag="PLS64_02375"
     CDS             complement(9821..9952)
                     /locus_tag="PLS64_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42713.1"
                     /translation="MNFNQNRNNNQNKNEQNSQNKNQNNNQNKNQQNSQNKSQNNCR"
     gene            10005..11327
                     /locus_tag="PLS64_02380"
     CDS             10005..11327
                     /locus_tag="PLS64_02380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRL42714.1"
                     /translation="MNDFLLRMKSLLGDEFDEFLKFYNSDDFIKGLRVNTLKCLPEKL
                     CSLLDFELKKTPFCDEGFYIPSDVKSIGNNPLHHAGAFYVQEPSATSAVTMLDVHEGD
                     YVLDLCAAPGGKSTQIGAKLNGTGLLWSNEIVRSRANILLSNIERMGISNAVVSNCRP
                     DELCKRLENRFDRILVDAPCSGEGMFRKNDAEREWSIEHVKSCAARQLLILNSAKDAL
                     KNGGYMVYSTCTFSKEENEGVISRFLAENPDFELVDSGVTFGRPTLDYARRIFPMDGG
                     EGHFAAKLHKKGEPYSNYNIPKKNNKIDSKIFDFYDSIFIDRPFGENIEVIKDKIIVL
                     PQNYNFDVKGLQILRAGVILGEIVKNRIEPHHSAFTAAKKENCKSAVDFDVNSKEIAA
                     YLHGEEIAVSQDVKGYTAVCVNGITTGFGKASNSRLKNKYPKGLRILR"
     gene            11324..12178
                     /gene="rsmA"
                     /locus_tag="PLS64_02385"
     CDS             11324..12178
                     /gene="rsmA"
                     /locus_tag="PLS64_02385"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRL42715.1"
                     /translation="MKKLSDIGTIKDILSRHGFTFSKSLGQNFLINPSVCPRMAELSG
                     AGEGVGVIEVGPGIGVLTTELCSLADKVVAVELDKRLLPVLEETLSEHDNVKVVNDDI
                     LKIDLHKLIEEEFQGMDVVVCANLPYYITSPVIMKLLEDRLPICAITVMVQKEAAQRI
                     CAEVGSRASGAVTVSVNYYAEPEMLFSVSAGSFMPAPKVDSAVLRLNILKEPPVKVDD
                     EKKFFKVVKAAFSQRRKTLSNSLASGLSLPKAEVNAILENSSVPLNARAEQLKLEDFA
                     NIANNLGD"
     gene            12181..12462
                     /locus_tag="PLS64_02390"
     CDS             12181..12462
                     /locus_tag="PLS64_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42716.1"
                     /translation="MKRKMNGLYKTAFILMLVAAVLFIVATIVAYALERPYFSAVFSC
                     SGAFLAFLGIIFATCSKPKKRKRHLVTDEIVAENDDINKISENSVDNEE"
     gene            12668..15265
                     /locus_tag="PLS64_02395"
     CDS             12668..15265
                     /locus_tag="PLS64_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRL42717.1"
                     /translation="MKNWYQLTESEALDKLGVTSEGLSSQQADSLLEKHGKNVLEESK
                     KKSVFQVFLSQFADLMVIILIIAAIVSMFSGSVESTIVIFAVITLNAILGTVQHVKAE
                     KSLESLKSLSSPSAKVIRDGRKIEIDSENIVPGDIVVLEAGDLVVADGRIINNYSLQV
                     NESSLTGESTNIDKGVEAIEKEVPLADRTDMVFSGSLVTYGRAIVVVTETGMNTEIGK
                     IATLMNQAKSKKTPLQLSLDKFSSRLAVIIMAICVVVFGLSMFNGMSVLNSLMFAVAL
                     AVAAIPEALGSIVTIVQAMGTQKMAKENAVIKDLKAVESLGCVSVICSDKTGTLTQNK
                     MTVQKIYVNGESILEDQLDLNIESHRHLLYDMVLNNDSSIVDGKGIGDPTEYALLETF
                     QHIGLDENVLRDVLTRVEEVPFDSDRKMMSTKYKMHGVNHILTKGALDSILDRCVSIA
                     TKDGIRPITDEDKAEISRVNREYSEQGLRVLTFAYKESDEALTVDTENDYTFIGLVSM
                     IDPPREESKQAVADAIRAGIKPVMITGDHKITASAIAKQIGIFNDGDIAVTGLELDAM
                     SDKELDEKIEKISVYARVSPENKIRIVEAWQRKGNIVSMTGDGVNDAPALKKADIGVA
                     MGITGTEVSKDAASMILQDDNFATIIKAVANGRNVYRNIKNAILFLLSGNMAGIFAVL
                     FCSIMALPVPFEAVHLLFINLLTDSLPAIAIGMEKPEKDLLRQKPRDPKQGILTKSFS
                     ALMLGQGALIAVVTLISFYIGLQTSPAVASTMAFATLTLARLFHGFNCRSTHSIFKLG
                     LFSNTASIGAFFIGTLLLAFVLFVPFMQPLFSVTALTGAQAGFIALLAFIPTFIIQFV
                     KVIVENVRK"
     gene            15386..15607
                     /locus_tag="PLS64_02400"
     CDS             15386..15607
                     /locus_tag="PLS64_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009534666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HRL42718.1"
                     /translation="MAFSIRLTEQEKKIADSYARLHSISLGEAFKQALFEKIEEEYDL
                     AVFDDAYDEYKKSGEESRPISDFWKELDI"
     gene            15604..15876
                     /locus_tag="PLS64_02405"
     CDS             15604..15876
                     /locus_tag="PLS64_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRL42719.1"
                     /translation="MSYVVRTTPRFEKEFKKLDRYTMKMIKAWIEKNLSGCENPRVKG
                     KALTANRKGQWRYRIGDYRLLCLIEDEELVILALSIGHRREVYDNT"
BASE COUNT         4829 a         3068 c         3436 g         4666 t
ORIGIN      
        1 aataggcata aattcctcga cgcctccaat taaaatgcct tgcatttcca agagcctttt
       61 cgtaaaatat ccgcagagcg tattttgtac cctgtttcgg atttaaacca attctatttt
      121 aacggatttt ggtagaatat gctctaattt cccacagtga aaaaattgtc aaattcggta
      181 attttgccta taacaaaatt atgcaaaatg tggtacaata atattgtaag aactcagtgt
      241 ttatctgaga tttaagatga tgcgattata cggatggtga gagtatgtac agtattggcg
      301 agattgttct ttacggctca aacggagttt gtgagattac cgaaattaca acgaaaagaa
      361 tcggaaagga ttctattgaa tactatgttt taaaacctgt ttgctcggat tcttcaacgc
      421 tttttgttcc gactcagaat gaaatgcttg tgtcaagaat gcgtgttgtc ctttcttcgg
      481 atgaaattaa agatattttg tcacagaaaa ccgataatga aatatggatt gacaataaag
      541 ccgaaagatg tgaaaaaatc aaggagatta tttcgggcgg cgattgtatg aagcttgttg
      601 agcttatcag gagaatgcac ttccacagta agctccagct caaaaagggc agacgacttc
      661 acattactga cgagcgtttt ctcaaagaag ctgaaaaaat ggtttgtgac gaggtttcgg
      721 ttgttcttca tattgaccgt aacgatgttc ttccgcttgt gcttgcaggc tgatttttta
      781 aaaaatatat aaaggataca gaggattaac cggagcattc tttgaattgc tttcaatgtt
      841 gttcctctgt tttttatttt tggtaattct aggttgtttg cctatttaca aaatgttgca
      901 ttttgtagta aaatatagga ataatgattt atgggagcta tttaatgaga aatttcaaaa
      961 aaactttatc atttttactt tcaacggtaa taatagtttc atcagttgca ggtgtcggta
     1021 caaccgtttt tgccgcaacc gatgaacaca catacaaaaa ctttaaatac agaattcttg
     1081 acgataacac agccgaaatc tgcggttata cgggcaagaa tgcagttgtg actgttcccg
     1141 acagcattga ttcaaagcct gtaacctcaa tcggcaggac ggctttttat tcaggcaaaa
     1201 tcaaggaagt gacaattccc aacacagtaa gaagcatcaa atggtgggct ttttacggtt
     1261 gtgaaagcct tgaaaaaatc aacctgaatt ttggtttaaa aactgtaggc tacggtgcgt
     1321 ttatgaattg taaaaatctt aaatccgtga gcattccaat gtccgtaact cagatttgcg
     1381 atgattcgtt tgcggtaagc tgttcaacca aaaagggcgt ttttgacacc tacagtaagc
     1441 agacaataag cactcagcag tattcaaccg attcatcgtt cactcttgaa ggctattcgg
     1501 ggactgtcgc agaaaaatat tgcaatgata attcgctaaa ctttgtttcg tcgggcaatg
     1561 tgatttacgg cgatgttaat aacaacggaa ctgttgaggc agctgatgca aagcttgcca
     1621 aaagccttat cgacactgtt ccgaccgagg aagaactgac ggctgccgat gttgacgatg
     1681 acggcaaaat cacagaaaat gatgttaatc ttattttgca ggctgtcggc aacgaatttt
     1741 acgcacagct ttttccgtgt gcaaaggcta tgtattctgc tcccgactac ctttcgggca
     1801 gaactatgta ttgtgacggc gacagcgttg cctacggttc gggtacggac acaatgggaa
     1861 attcaacata ttctttctgc aattatgttg ccgaaaaata taatatgaca atgaccaaca
     1921 aggccactcc cggcacaact cttgcaataa gaaaggattt tatcggcaca gaccacagaa
     1981 gtatccttga gcgtgtccgt gaaatgaaag gcagttacga tgttattctg cttgacggcg
     2041 gttttaatga cctgttcaaa aatgttgaaa tgggtgcaat gaccgatata aataataaat
     2101 cgggaaaata caatgaatac accacagcag gtgcgttgga aagtatttgc tattttcttg
     2161 acaagaatta taaagactca attaagctgt tcgtactctg tcacaattgc tcaacaagaa
     2221 caaaacagtc acagtattgg tctttaatga aaaatatact tgataagtgg gaaattccct
     2281 atgttgacct ttcggaagaa acagagctta ccggtgataa tgaggaaatt acaacacagt
     2341 attttagata taatgcaaca accaaaaaag gtgacgggat tcaccctctt gcatatgcca
     2401 atatgaagat atacggtccc atcgttgccg aaaagcttaa cgaaacagta cagagcaagt
     2461 ctgaacttgt tttgccgaaa agtgacattt ctatgggact ttttgaaagt tacaccctta
     2521 acagcgaaat tacggagttg aggggagata tagatgtcag ctattcaagc agtaacccgt
     2581 cagttgcctc tgttgatgaa aacggcaata ttgttgccac gggcataggc gatacggtta
     2641 tcacgatttc gacatctgac ggtaaaacaa aaaatgtgaa tgtgaatgta aagtttcttg
     2701 caatggcggt tagctttggc aaaaataaaa tttcactttc agagggtaat tcaagcttgc
     2761 tgaatctttc tgtagcagac ggtgaggcga cctgttcaac aacctattcg tcaacagatc
     2821 cgactgttgc gtctgttgat gaaaacagcg gaaaaattac agccaacaaa acgggcaaaa
     2881 ccacagtatc atgtaccacg gcaaacggtg taacggtaag gtgccttgta tatgtcacaa
     2941 gcagtgcgct gactaaaatg cagaaagcgt aaggcgttta tgaaaatatt taacagaaaa
     3001 ttaaaaataa cttcatctgc tttgcttaca ttatgcatgg tttttgtaat gacggcttgt
     3061 gccgaaaaca gcagtcagtc cgaaaaatca cagcctgcag agcagacgac tgttcagccg
     3121 acaacaatgt cagccgaaga aattaatgac cgaaagcttg ataaattcat agctgatatg
     3181 acgcttgaag aaaaggtggg gcagatgttt tttgtacgct gccccgatga agatgctgtg
     3241 caacaggtca gcgagtacaa tatcggcggt tacatcctgt ttggcagaga ttttgacggt
     3301 aaaaccaaag atgaagttgt tgacgatatt cacagctatc agaacgaggc ggatattccg
     3361 cttttaatcg gcgttgatga ggagggcgga acggttgtgc gtgtcagcag taacccaaac
     3421 ctccgtgaaa ctccgtttct ttctccaaaa gatacttatg cagacggcgg ttgggatgct
     3481 gtcaagcatg atgcagaaga aaaagcgaat ttactccttt cactcggaat caatgtgaat
     3541 ctggctcccg tgtgcgatat gacctcggat gaatacggct ttatgtatga ccgttctttc
     3601 agctcggatg ttgatatgga aaacagatat gtgcgaaccg ttgttgaaac aagcaaatca
     3661 aaaaagctcg gcacggttct gaagcatttt cccggttacg gcaataacag cgacacacac
     3721 acgggaattg catacgatga ccgtgactat tcggaatttg aaaatacgga ttttaaaccg
     3781 ttttatcagg gaattgagtc cggtgccgac tgtattcttg tttcacacaa catagttaat
     3841 tgcatggaca gcgaatatcc tgcgtcactt tcacaaaagg tgcatgatat tctcagaaat
     3901 gaattcaaat ttgacggtgt aattatgacc gatgacctta tcatggacgc aatcacggat
     3961 tttacgggag atgaagcggc ggcagtcaca gcggcaaaat gcggtaacga tttgctctgt
     4021 tgttcatctg tcgataccca atatcccgca gttcttgagg ctgttcaaaa cggtgaaatt
     4081 cccgaagctc aggttgacgc atccgtaaag cgaatcctga aatggaaaca aaatctcggc
     4141 atttttaata ttgacaccta tcagaaaaaa gtcaaatcaa ccgaaccaac ggataccgtt
     4201 ggaagtgagg agtgaaaagt taatagctgt taggtgcggt cgggaagata gtttgaatcg
     4261 tttatatcat attttgcccg agtatgcgac tgcaaaaatt cttacggtaa aacataaaac
     4321 accccgaaga ttaagttctt cggggtgttc tgtttgtaaa tgagtgtcaa agtatatcga
     4381 catatttcaa tagatgtgat atactgattg tgctgttgac tctttccggc aggaaggagg
     4441 tgacccaatg gatcttctca tttcattttt attatctatt ggagcaagtg tagttgccta
     4501 ctacatttgc aagtggttgg atagataatg cttgaacagc acaacctaag aagaaaccct
     4561 caaagctacc gactttgagg gtttcgtttt gcccaatgga caactcattt cattttgcct
     4621 acaattattg tagcacacat ttctgtgcat ttcaagtata tgccgaaaaa ttattattat
     4681 tcctgattag aattattgaa cacttttaca agcaaagatt caaagctttt ttcaaagttt
     4741 tcgtcatctt gttttgtacg ctttttcggt cctttaatgt gatgcttgcc gtttgacatt
     4801 ctcgcctttt tggcaaggtg cctgtccata agcagaccgc ctatgttatc atccgagaaa
     4861 atttttcccg attgatgaat acagtacgca ccctttccaa gcacaagtgc cgcaactccg
     4921 taatcctgag ttaccgcaat gtcgcccttt ttacaaaggt tgacaagcgc caaatcaact
     4981 gcgtctgcac ctgcaccgat aatttttatc tcgctgtaat ctgactttag aacatggttt
     5041 gtgtcacaca aaagcacaac gggaacattg tgcttttttg caatcctttc aaccgatttt
     5101 acaacagggc aggcatccgc atcaacaaga acagttatac cgttcattat ttttcctcaa
     5161 tcttaattga gttgataaca ggctgatttt tcttttcaac agtaccgttt gaatctgtaa
     5221 caggtgtgtc ctcgcagatt ttatcaacaa tatccatacc ctttgttacc ttgccgaatg
     5281 cggcgtactg accgtcaaga tatgtgctgt cagcctgaac aataaagaac tgtgagcttg
     5341 cactgtcggg ttcatttgaa cgagccattg agataacgcc tcttgtatgt gaaaggttgt
     5401 tttcaacacc gttctgagca aattcgccct tgattgtttt ttcactgccg cctgtgccgt
     5461 caccctttgg gtcaccgccc tgaatcataa agccggtaat gattctgtgg aaagtaagac
     5521 cgtcataaaa accgtctttt gcaaggtcaa caaagttctt gactgtaatc ggagcaagct
     5581 cctcataaag ctcaacttca attttaccgt agtctttaac atcaatagtt gctgtgtagc
     5641 ttgatttgta gtcggttgtg tctgtgcttg cggatgttgt ttcttcgttt gaaactgcag
     5701 aagttgcttt ttcagaagaa ctgctctctg tgtcaccgcc gcaaccgctc attaaaaagc
     5761 atgtgcagag aataactgcg gcaaccataa ttgccgaaat ctttttaatt tttgtcattt
     5821 tcaaattcct ttcataaata aacttaattt attttatcat tattatgtct gcaaatcaac
     5881 ctgaattgac acaaaaatat aaatgtgtta aaattttttg tatgtgaaat accgacaaaa
     5941 ggggaatgtg gcttgaaaaa taatgaaata accgatatta cttcaaaacg cagattgttt
     6001 gagcttaatc tcggtgatgt ccgaaagtac cgtgaccttg ttatgctgtt cgtaaagcgt
     6061 gattttaaaa attcctacaa gcaaaccgta ctcggaccgt tgtggataat aataaagccg
     6121 ttcctttcaa catttgtttt caccgtaatt ttcggtatga ttgccaatat ctcaactgac
     6181 ggaatgcctc aattcctgtt ttttatggca ggcaacattt tgtggtcgtt tttttcaagc
     6241 tgtcttaacg gcagttcatc aacctttatc ggcaacgcaa ggctgtttgg caaggtgtat
     6301 tttccaaggc ttgtaatgcc gtgcgcaaac ataatttaca acacaatcgg ttttttgatt
     6361 aactttgtaa tgttctttat acttgttctt atatatatgt ttacgggttc aaatgttcac
     6421 ccgaatttat gcgcattgct tatgcctttg attctgcttt cttcggcact tttaggcaca
     6481 ggctgcggac tgattgtttc gtctgtaaca acaaaatacc gtgacctcgg tgtgcttgtt
     6541 tcattcggag taacattgtg gatgtatatt actcctgttg tttatcctgt ttctcaggtg
     6601 tcggattcat tccgtccgct tgcactctta aatcctgttg ctccgctaat tgaaacattc
     6661 cgttacggat tcttaggcag cggaacattt ccgtggcaat atctcatatt gagcttcgct
     6721 gttacggcag tcgttcttct gttcggaata atcgtcttta atagggtaga aaagaatttt
     6781 atagatacgg tatgatgtca tgactgaaat gaatgaaaat tgcgtaataa aaattgaaaa
     6841 tctttcaaaa gaataccgac tcggtgcaat cggttcggga actctccgtc atgatttgca
     6901 gagttggtat gcaaaaaaaa gaggtaaaga agatccgaat cagagaatcg gtgccaaaca
     6961 atacacaaaa ggcgacacat ttttagcact tgacggtgtg aaccttgagg taaaccgtgg
     7021 agaacgcata ggaattatag gcgctaacgg tgcaggcaag tcaactctgt taaaggtttt
     7081 atgcagaatt acctctccga cagacggcag agtgcgtttc aggggacgaa ttgccagtat
     7141 gcttgaggtc ggaacaggct ttcatgctga gcttaccggc agggaaaaca tctaccttaa
     7201 cggtgcaatc ctcggaatga caaagaaaga ggtcacttca aaaatagaca gtattattga
     7261 attttcagag tgcgaaaagt tcattgacac gcccgtaaag cgttattcat cgggtatgtt
     7321 cgtaaagctt gcgtttgctg ttgcggcaca ccttgatgca gaaatcatga ttatggacga
     7381 ggtgcttgct gtcggagata tgcactttca gaaaaagtgt atcgggaaaa tgcgtgacct
     7441 tgcaaccgag gaggacagaa ctgtcctcta tgtttcacac aatatgaata ctattcgtga
     7501 cctttgcgac aggtgcatag ttcttgacca cggcaaaatt gtttttgacg gcgatgttga
     7561 agacgctgta aaaatctatc tcggaagtgc aaacgaaaat ttccgtgcca ttgaatatgt
     7621 cggaaacgaa cacaagcatc atgccgacag aaacgacctt tgtctgcaat ttgccggata
     7681 taacggcaaa gaggataata ttttctatga tgacgagcag attttgcttg aactcacatg
     7741 gaaaaacaag gctgatattg aaaatctttg tctgcgtgtt gaagtgagcg attaccgaag
     7801 atatcccgtt accgcatttg tggttgaaaa tttctattcg ggaaaaaagg gcgaaaccca
     7861 cacggcaaaa ttgaaaatag acatatcaaa aattgtccgt ggcggatact atacacgcta
     7921 tgttttttac agccgaaaga acgatgccgc cccctttgaa accttagagg tcgcagacgg
     7981 tctgactttc agaagaagaa aacccgataa ataccaaaac acctggcaaa aagcctgggg
     8041 cagtattgaa atgaatgata ttgaagttgt ataaaaacaa attgaaattt gcggttgggc
     8101 aaacagttaa tatcaaaaga aatatgtgtt ccgaatgttg tgaacatcta attgaaatgt
     8161 aatgaaagaa acaatatgag cgaaagatct gattattttt aaacgcatat tttgatgaat
     8221 aacaaaaggt cagcacataa tcgtgttgac ctttattctt tgtcaaaatt aaattacggc
     8281 tcgacaaacg gcatattaaa atactcggca actgattttg caagatttct gccgattgca
     8341 ttaatgttgt ccctaatcca ttgagcttct tcggggttat cgtgatatgc aacctcaatc
     8401 aatgccgacg gtgattttgt ccgtctgact tccgcaagtg aggtggtggg gattatgtca
     8461 acatcgttcg gttcgggata aatatccttg taattctttt gcagaatttc agcaaaacgc
     8521 ttgccgttac tgcctgtcgg atagtaataa atatctgcac ccgtgtatct gcctgccgaa
     8581 ccctcgggag cggcattgct gtgaattgca aggtgcaaat cgtgattgcc cttgtttgaa
     8641 tctgcaattg cctgactcag cgtcatatcg ggatcgtttc gcctgaaatc tattccgctt
     8701 gccctcaaat aaggctccat ggcgtctgca ataagattca tataatattc ttcatttcct
     8761 ccgtcaatat aagggttgta ctcctgcaat gacggtgaaa gataaactga cggcataaaa
     8821 tctctcctaa aattaatctc ttatcatttt attcgcaaaa cccgtgccgt tgacaaataa
     8881 ttttcttttc aaaattcaat ttgtattgta taatgattac atatgatttt ggtgaaaatg
     8941 ttggcaagtg tatgatatca caattattat gtgtgagcag attgtaaccg taggcagaat
     9001 gtcaagcgta tgggcggata ttgttcattt aaagaactat tatttaatac aaaaacttgc
     9061 ttaattgctt tgtgaaatta tgaaacggag aaattattat gactgatgaa atgagagcaa
     9121 aagttgagaa agttatgaaa aatcttgaac gcaacaagat gaaaccttat ttttgcgaaa
     9181 acagggaaga ggctcaggca cttgtaaaaa cactcattaa aaagggcgaa acaatttcat
     9241 gcggaggttc aaaaactctc gacgaaacgg gaatttatca gataattaat tcacccgatt
     9301 acaattttct tgaccgttcc gcacccggaa tgactcgtcc cgaggttgaa gaggtttacc
     9361 gtcagacttt cagggcagac acatttttta tgagcacaaa cgctcttacc gaaaacggag
     9421 agctttataa tgttgacggc aactcaaacc gtgttgccgc acttctttac ggtccgaaaa
     9481 gcgttatcgt tgtatgcggt atcaacaaaa ttgtaaagaa tattgacgag gcaatcaaga
     9541 gagttaaaac aattgccgct ccgcagaata caatcagact cggcattgaa accccctgcg
     9601 gaaaaacagg cgaatgtgtt tcattgagaa aagaaaatcc cgagctctgt gacggttgtc
     9661 acggtgacac aagaatctgc tgtaactatg tggtatctgc tcagcaaaga catattgaca
     9721 gaatcaaggt tattatcatc ggcgaagaat acggttatta attcccataa agaaaaaagc
     9781 cgtatcaaaa gacacggctt gggagaactt cagaatgtaa tcatctgcaa ttgttctgac
     9841 ttttgttctg gctgttctgc tgatttttat tctgattgtt gttctgattc ttattctggc
     9901 tgttctgctc gttcttgttc tgattgttgt tccgattctg attgaaattc atttcaacac
     9961 ctcctttaca aaactattat taacccgaaa aggcttgttt attcatgaat gattttttat
    10021 tgcgaatgaa atcacttctg ggtgatgaat ttgacgaatt tttaaaattt tacaacagtg
    10081 acgatttcat aaagggactt cgtgtcaaca ccttaaaatg tttgcccgaa aagctttgtt
    10141 cacttcttga ttttgaactg aaaaaaactc cgttttgcga tgagggcttc tatattccgt
    10201 ccgatgttaa gtcaatcggc aacaatcctc tgcaccatgc aggagcgttc tatgttcagg
    10261 aaccgtctgc aacttcggca gttacaatgc ttgatgttca tgagggcgat tatgtccttg
    10321 atttgtgtgc cgctccgggc ggaaaaagta cgcaaatcgg cgctaaactt aacggaacag
    10381 gacttttgtg gagcaatgaa attgtaagaa gcagagcgaa tattctttta tcgaatattg
    10441 aacgaatggg tatttcaaac gcagttgtat caaactgccg tcccgatgaa ctttgcaaaa
    10501 ggcttgaaaa ccgttttgac aggattcttg ttgacgcccc ctgcagcggt gagggaatgt
    10561 tccgcaagaa tgatgcggag cgtgaatgga gcattgagca tgtaaagagt tgtgccgccc
    10621 gtcagctttt gattcttaac agtgcaaagg atgccctgaa aaacggcgga tatatggttt
    10681 attcaacctg tactttttca aaggaagaaa acgagggcgt aatttctcgg ttccttgcag
    10741 aaaatcccga ttttgaactt gttgattcgg gcgtaacatt cggcagacct acgcttgatt
    10801 atgcgagaag aattttcccg atggacggcg gagaaggaca ttttgccgcc aagcttcaca
    10861 aaaagggcga gccgtattct aattataata ttcctaaaaa gaataataaa attgactcga
    10921 aaatttttga tttttatgac agtattttta tcgacagacc gttcggtgaa aatattgagg
    10981 ttataaagga taaaattata gttcttccac agaattataa ttttgatgta aaaggcttgc
    11041 agattctccg tgcgggagtt attctcggcg aaattgtcaa gaacagaatc gaaccgcatc
    11101 acagcgcatt tacggcagct aaaaaggaaa actgtaaatc cgcagttgat tttgatgtga
    11161 acagtaaaga aattgcagcg tatcttcacg gtgaggaaat tgccgtgtca caggatgtca
    11221 aaggctatac ggctgtctgc gtaaacggaa tcacaacagg ctttggcaaa gcgtcaaatt
    11281 caagactgaa aaataaatat ccgaaagggc ttcgcatatt aagatgaaaa aattatcaga
    11341 tataggtaca atcaaagata tactttcaag acacggcttt actttttcaa agtcactcgg
    11401 tcagaatttc cttataaacc cgtcagtttg tccgagaatg gcagagcttt cgggcgccgg
    11461 agagggagtt ggcgtaattg aagtcggtcc cggaataggg gttctcacaa cagagctttg
    11521 ctcacttgcc gacaaggttg tagcggttga gcttgacaag cgacttcttc ccgttcttga
    11581 agaaacactc tccgaacacg acaatgttaa agttgtaaac gatgatattc ttaaaattga
    11641 cttgcacaag ctgattgaag aagaatttca gggtatggat gttgttgtgt gcgcaaatct
    11701 tccgtactac atcacatctc ccgtaattat gaaattgctt gaggacagac tgccgatttg
    11761 tgcaatcacg gttatggttc aaaaagaggc ggctcagcga atttgtgctg aggtgggttc
    11821 tcgtgcaagc ggtgcggtaa cggtatcggt caactattac gcagaacccg aaatgctgtt
    11881 ttcggttagt gccggctcgt ttatgcctgc tccaaaggtt gattcggctg ttttaagact
    11941 taacattctc aaagaaccgc ccgtgaaggt tgatgacgaa aagaagtttt ttaaggttgt
    12001 aaaagccgcc ttttcacagc gaagaaaaac actttcaaat tctcttgcat ccggactttc
    12061 tttgccaaag gcagaggtca acgcaattct tgaaaattcc tccgttcctc taaatgcaag
    12121 ggcggaacag cttaaacttg aagattttgc aaacattgca aacaatcttg gtgattgatt
    12181 atgaaaagaa aaatgaacgg tctttataaa accgctttta tattaatgct tgttgccgca
    12241 gtgcttttca ttgttgcaac aattgttgca tatgctcttg aacgcccgta tttttccgca
    12301 gtcttttcat gcagcggtgc ttttcttgca tttttgggca taatatttgc aacctgcagt
    12361 aaacccaaaa aacgcaaaag acaccttgtc acagatgaaa ttgttgccga aaatgatgat
    12421 atcaacaaaa tttcggaaaa ttctgttgac aatgaggaat aaatttgata gaatggttgc
    12481 agataaaggg gagtagttcg cagataatct gtgatattaa tcgtcagtac ataccgtttt
    12541 cggtatcggt tatatcttaa aggacaagac ttttattgta gggtgagttg caccttgcaa
    12601 taaaggtctt ttttatttgc ctttttcagt ctgtctgtat tctgcaatcc gaacggagga
    12661 gaaaattatg aaaaattggt atcagttgac cgagagcgaa gctcttgaca agctcggtgt
    12721 cacaagcgaa ggactttcgt cacaacaggc tgacagcctt cttgaaaaac acggaaaaaa
    12781 tgtgcttgaa gaaagcaaaa agaaatctgt ttttcaggtg tttttaagtc agtttgccga
    12841 ccttatggta ataatcctta taattgcggc aatcgtttca atgttttcgg gaagtgttga
    12901 aagcacaatc gtaatttttg cggtaatcac acttaacgct attctcggca cggttcagca
    12961 tgtcaaggca gaaaagtcac ttgaaagcct taaatcgctt tcgtcaccct cggcaaaggt
    13021 aatccgtgac ggaagaaaaa ttgaaattga ttcagaaaac atcgttcccg gcgacattgt
    13081 tgtgcttgag gcaggcgacc ttgttgttgc agacggaaga ataataaata actattcttt
    13141 acaggtaaat gaaagctcgc tcacgggtga atctacaaat attgacaaag gtgtggaggc
    13201 aattgaaaag gaagttccgc ttgccgaccg cactgatatg gtcttttcgg gcagccttgt
    13261 aacatacggc agagcaattg tcgttgttac ggaaacaggc atgaacacag aaatcggtaa
    13321 aattgctaca cttatgaatc aggcaaagag caagaaaact cccctccagc tcagccttga
    13381 taaattttcg agcagacttg ccgttattat tatggcaatc tgtgtagtag ttttcggctt
    13441 gagtatgttc aacggaatgt cggttcttaa ctctcttatg tttgccgttg cacttgcagt
    13501 agccgcaatc cctgaggcac tcggctcaat cgttacaatt gttcaggcaa tgggtacgca
    13561 aaaaatggca aaagaaaatg ccgttatcaa ggatttgaag gctgttgaaa gcctcggttg
    13621 tgtgtctgta atctgctccg ataagacggg tacgctcaca cagaacaaaa tgactgttca
    13681 gaaaatctat gtgaacggcg aaagtattct tgaggatcag ctcgacctta atattgaaag
    13741 ccaccgtcat ctcctttacg atatggttct aaacaacgat tcgagtatcg ttgacggaaa
    13801 gggaataggc gacccgaccg agtacgcttt gcttgaaaca tttcagcata tcggacttga
    13861 cgaaaatgtg cttagggatg ttcttacaag ggttgaggaa gttccgtttg attccgacag
    13921 aaagatgatg agcacaaagt acaaaatgca cggtgtaaat catattctca caaagggtgc
    13981 gcttgattca attcttgacc gttgtgtaag tatcgcaaca aaagacggta ttcgtccgat
    14041 tacagatgaa gataaggctg agatttcaag agttaaccgt gaatattccg aacagggact
    14101 ccgtgttctc acctttgcat ataaggaatc tgatgaggct ctcacggttg acacagagaa
    14161 tgattataca ttcatcggac ttgtttcaat gatagatcct ccgagagagg agtcaaaaca
    14221 ggctgttgcc gatgcaatcc gtgcaggaat caagcctgtt atgattacgg gcgaccacaa
    14281 aatcacagca agtgcaatcg caaagcagat tggaattttc aatgacggcg acatcgcagt
    14341 tacaggtctt gagcttgacg caatgagcga taaggagctt gacgaaaaaa ttgagaaaat
    14401 ttctgtttat gcacgagttt ctcccgaaaa caaaatcaga atcgttgagg cttggcaaag
    14461 aaaaggcaac attgtttcca tgacaggtga cggtgtaaac gatgcacccg ctctcaaaaa
    14521 agcggatatc ggtgttgcaa tgggtatcac aggtaccgaa gtttcaaaag acgccgcatc
    14581 tatgattctt caggatgata actttgctac aattatcaag gcggttgcaa acggcagaaa
    14641 tgtttaccgc aacattaaaa atgcaattct tttcttgctt tcgggtaata tggcaggcat
    14701 ttttgccgtg ttgttctgtt caatcatggc tctgcccgta ccgtttgagg cggttcacct
    14761 tctgtttatc aaccttctta cagactcact tcccgcaatt gcaatcggta tggagaagcc
    14821 cgaaaaagat ttgctcagac agaaaccccg tgatccgaaa cagggaatcc ttacaaaaag
    14881 cttttctgct cttatgctcg gacagggcgc tttgattgcc gttgtaacgc tcatttcatt
    14941 ctacatcgga cttcagacaa gtcctgccgt tgcaagcaca atggcatttg caacactcac
    15001 gcttgcaaga ctgttccacg gctttaattg cagaagtaca cattcaatct ttaaactcgg
    15061 acttttttca aatacggcaa gcataggcgc tttctttatc ggaacattac tgctcgcatt
    15121 tgtattgttt gttccgttta tgcagccgct tttcagcgtt accgcactca caggcgcaca
    15181 ggcaggcttt atagccctcc ttgcattcat cccgacattt ataattcagt ttgtaaaagt
    15241 cattgttgaa aatgtgagaa agtaaattta ataacattga acacggcaga ttgattttgc
    15301 cgtgttcttt tacttttatc gaataaaatt cattgacaaa gaaataataa tgtagtacaa
    15361 tgtactatac aaggaggtca gtattatggc attctcaatc agattaacag aacaggaaaa
    15421 gaaaatagca gacagctatg ctcggttgca ctcaatatca ctcggagagg cttttaaaca
    15481 ggctttgttt gaaaaaatcg aagaagaata tgaccttgcc gtttttgatg acgcttatga
    15541 tgaatacaaa aagagcggtg aagaaagcag accaatctct gatttttgga aggaacttga
    15601 catatgagtt atgttgtaag aaccactccg agatttgaaa aagaatttaa aaaacttgac
    15661 agatatacca tgaagatgat taaagcgtgg attgaaaaga atttgtcggg ttgtgaaaac
    15721 cccagagtaa agggaaaagc tctcacggca aatcgcaaag gtcaatggcg gtatcgtatc
    15781 ggagattaca gactgctctg ccttatagaa gacgaagaac ttgttattct tgctttgtct
    15841 ataggtcaca ggcgagaggt ttatgacaac acttaaaatt taaaacactt aaataatcaa
    15901 actcggtcat aatctttgaa taattatgac cgaaaattat ttattttgaa ttattttgat
    15961 tgaaattaca aaaattcctt gacaaagttt gacagattt
//
LOCUS       DAOMVE010000023        15957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_744_length_15957_cov_2.871588, whole genome shotgun sequence.
ACCESSION   DAOMVE010000023 DAOMVE010000000
VERSION     DAOMVE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15957
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_744_length_15957_cov_2.871588"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PLS64_02410"
     CDS             <1..509
                     /locus_tag="PLS64_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HRL42720.1"
                     /translation="GKFGKKGSMISAVDLLKGIAICAGMKSVDVEGATGYIDTNFDGK
                     CKAAIDEFKNGADFVYIHVEAPDECGHRGEIENKVKAIELIDEYILKPVTDFLRTFDD
                     FAVLVCPDHPTPLSIRTHTSNPVPYLIYDSKNEVDSGVSKFCEEEAKKTGNYIEKGFT
                     MMDYFLSK"
     gene            complement(713..967)
                     /locus_tag="PLS64_02415"
     CDS             complement(713..967)
                     /locus_tag="PLS64_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HRL42721.1"
                     /translation="MKYSYFPTGVCSRQINLELDDNGKITSCEFVGGCSGNTQGICML
                     VKGMDAKDAVDKLKGIDCRGRGTSCPDQLSKALVEALKLI"
     gene            1240..2337
                     /locus_tag="PLS64_02420"
     CDS             1240..2337
                     /locus_tag="PLS64_02420"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRL42722.1"
                     /translation="MSINIDLGAWNSIFAVPSKVVDEGLKFSDGVKLKVLLFVLRNAG
                     RSVDDTEISKATGVNVTDIPEALDYWVSMGVFNRIENTFTPVKNNEEFVSAPQTSVQT
                     EQTPQPVQTVLQPQQTVPQPVQAVQEPVKQETEQSPKENAEEPQKHFTATRPQKPDFV
                     FTAQRLAVDEELKMLVEETQTTLGKVLSNSDIATLLMLKDTCGLPLDVIFMLIHYCAS
                     IDKGNIRTIENIGIQWANDGVYSLEAADNKIKQIQKTTANFSIVSKAFGLKNVGSPTK
                     KQLEYGDKWVSEWKFSPEMLREAYERCVDTKGTMNLRYIDGILKRWNASNLHTLDELH
                     KYEKSASKPSQKQSSSYDINELDKFNSLDNF"
     gene            2351..3337
                     /locus_tag="PLS64_02425"
     CDS             2351..3337
                     /locus_tag="PLS64_02425"
                     /inference="COORDINATES: protein motif:HMM:NF005304.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL42723.1"
                     /translation="MGYSNSVYKTASDTLHERRLNAEKAAERRKEEVYKKFPRVKELE
                     KQISVGGIKTARAVLAGGDVKDAVSKLRDQNLAMQAEVRKILTDNGYPENYFEPDYFC
                     KKCNDKGYCDINGKTVRCSCMKSALIACACAELNRNAPLSLSTFESFNLEYYDKTVDP
                     KLKVSPYDHMSRVLRYCQNYADNFNSHSESILMKGATGLGKTHLSLAIANEAIKRGYG
                     VIYASAPQLVSKLEKIYFSNKEDDSTFDMLVECDLLIIDDLGTEFHSQFSVSQLYNIF
                     NSRMLSNKPVIINTNLTIRDLQENYTDRFVSRICGNARQLDFLGRDIRIRRK"
     gene            3487..3560
                     /locus_tag="PLS64_02430"
     tRNA            3487..3560
                     /locus_tag="PLS64_02430"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3519..3521,aa:Gln,seq:ctg)
     gene            3593..3669
                     /locus_tag="PLS64_02435"
     tRNA            3593..3669
                     /locus_tag="PLS64_02435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3627..3629,aa:Pro,seq:cgg)
     gene            3734..4198
                     /locus_tag="PLS64_02440"
     CDS             3734..4198
                     /locus_tag="PLS64_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HRL42724.1"
                     /translation="MKKVISLAVALVLCLSIFAGCGAKEVNLADLMDKMNSEYSVDAT
                     KYETKDDMYKYYNINADDIKQFAAEVGKSDTDSENTEVVLVEATDSDAASRVETALTN
                     RYNSIFQQYASYSAEKLDMVKNCKVTKDGNFVTMIIGEKASDMLKMFNDSIK"
     gene            4270..6519
                     /locus_tag="PLS64_02445"
     CDS             4270..6519
                     /locus_tag="PLS64_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL42725.1"
                     /translation="MVQTEIQKNFSHMNDMQKQAVFCTEGPLLILAGAGSGKTTVLVN
                     RIAYILQCELCKPWQILAITFTNKAAGELKERICAAVPEGGADIWAATFHSTCARILR
                     RYGDRIGYTSHFTVYGTDDQKKLVKDILKQLNIAEKTLPVKSILSEISKAKDKMLTPE
                     EMRKEAEFDSRKAQIAKVYEIYQTKLKTADAMDFDDMLCNTVKLFETAPDILDYYRNQ
                     FKYIMVDEYQDTNKVQYKFVSLLASKYGNICVVGDDDQSIYKFRGATIENILSFENTF
                     KNAKMIRLEQNYRSTQNILNAANEVISNNTMRKGKTLWTENGQGEKIKVHTAENERDE
                     ANFIAQTILDGVADGRKYSDYAILYRMNAQSNAIEQALSRSGVPHRVIGGHRFYDREE
                     IRDMVAYLQVINNPHDDVRLSRIINVPKRGIGATTVSHAADIAAGLGESIYDVIKEAE
                     NYPQLSRASAKLKAFVALIDGLIEAEQSGEYSLAELYNLVIEHTSYEQYLKTEKDNPE
                     VRIENIEELSSNIVKFEEDYGDEADLSAFLEEISLQTDIDNYDADADTCVMMTLHSAK
                     GLEFPVVFIAGMEEGMFPSVATMMNPDELNEERRLAYVGITRAKEILYFTKTNSRMLF
                     GSTSYNKGSRFLNEIPDNLLEYSGGRKQMFTQAASGFASGTGEKVSVGKSSGSSYSPN
                     KSFGFTKPPVKSGAVFNVGDCVQHKVFGKGMVMKAEKMGNDTMLEIAFDKAGTKTLMA
                     NYCSNMKKI"
     gene            6530..7741
                     /locus_tag="PLS64_02450"
     CDS             6530..7741
                     /locus_tag="PLS64_02450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRL42726.1"
                     /translation="MLISSKKVLCLAISALTAFSVCLTGCGKDTQSSVSATEKTTAVV
                     ASQPVTKAATSDEQTQEPTWNYSELKRKSSDISENLDAIITDNKFRGTVYVKIGNALE
                     YIGTNGFSDKDRHTKNSTDTCFRIASLTKQFTAVAVMQLVEEGKLSLDDTIDKYFPSY
                     KYGKKITVKNLLTMTAGIKDYINKDGDTNTYAYNESQLEYKVSAENSAKKNKKAVMDW
                     ILDQELNFEPDEKYMYSNSAYFLLGDIIEQVSETSYEKYIKENILKPSGMMNTGFEGT
                     DKLAVGYQDIYDNAWTLYPGVGYSATGLISNVPDLLKWVDALCTNKLISEKSFKEMTT
                     PYKGNYGYGFVVSKDSNMISHTGKIDKYNAALAFTKDENQIYIALSNYSNSSPINLFN
                     NIQKTLAPFYG"
     gene            7838..8704
                     /locus_tag="PLS64_02455"
     CDS             7838..8704
                     /locus_tag="PLS64_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42727.1"
                     /translation="MSDVPFCSSENACTEGGEQTNGCEPVCINVSRIYDSCGAKDCLS
                     NLPVMFTEANQKIIDGACSVKISNVRIITSTVEVEPVAFHRGFYSVDMMYYFAVTVEA
                     YTAAGAIPETVTGLAMYGKRVVLYGGEGCVKSFSSDKDVVCDTSDSDCPCKYPYCLPR
                     ATVQISNPMALSANLQDYNNANPITVCRTFPSCVIDYLDGEPAQPETQRVLVTIGIFT
                     ITRLERDVQIMIPSYDFCVPRKECPSAPDDPCEVFSKIDFPTDSFFPPNAIHRGDRDS
                     SHTSSFKCGCEN"
     gene            complement(8748..10112)
                     /locus_tag="PLS64_02460"
     CDS             complement(8748..10112)
                     /locus_tag="PLS64_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL42728.1"
                     /translation="MLYDFIIINKKDKTPLYRQIYMSVRKSIETGSLKKGTKLPSIRR
                     LSTDLDVSKTTVNGAYEQLCAEGYISNRPRSGYYVEAQFSGTPSKSDKMSSNTNKTKR
                     YYEYDFSSKSIDDGIININEWKKYVKEVLNQNYLLTSYGEEQGEEILRNALQKYSLGT
                     RSVNTSYENIIVGAGTQTLLHILCSLLGNNKKIALADYSYIQSEYVFNSHGYDIKYFR
                     CDKYGACIDSLEEIKPDIILINPNFTVNSNITMPVTRRLEIIKWAKENNVLIIEDDYN
                     GELRYSTHPMPCVQNYDTENTVYLGSFSKILLPSVRISYMVLPQKLTDEYNKIKKYTN
                     QTASKTEQIALAKYINNGKLDVHLRKARRIYLEKSNLMLSNIKKYFGTSVKIVFNETS
                     LYVSLIFKDKTIDDSVIKKIDDALIKTMQFNKAENMIVLSFSGIKDVKIESGIKLLYE
                     TISK"
     gene            10265..11656
                     /gene="murD"
                     /locus_tag="PLS64_02465"
     CDS             10265..11656
                     /gene="murD"
                     /locus_tag="PLS64_02465"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRL42729.1"
                     /translation="MNAMADKFFDSIKGKKVTFIGIGTSNLPLIKLFVEKGAKVTACD
                     KKNFEDLGQNGIKAKEYGAELLLGENYLSEIDADIVFRSPGTPFYKDELVALRNKGVV
                     LTSEMEVFFDLCPCRIIAVTGSDGKTTTTTIISEFLKAAGFNVHLGGNIGKPLLPEIE
                     SVNDDDCAVVELSSFQLISMRKSPDIAVVTNLAPNHLDIHKDMQEYIDSKKNIILHQN
                     AFSKAVLNLDNEITNGFSDSVRGQLAKFSVKENVSNGAFLDGDTICYNDYGKITRIMN
                     IHDIKIPGMHNVENYLAAISAVWGLVDAETMTAVAKTFGGVAHRAEFVREVDGVKYYN
                     DSIASSPTRTALGTLSLYSRKICIIAGGYDKHIPYEPLGPVINDKVKLLILLGDTAPK
                     IEKAVKEASNYDADEIEIVNVTNMEEAVAVARERTTKGDIVSLSPASASFGLYKNFEE
                     RGNHFKSIVNALK"
     gene            11665..12693
                     /locus_tag="PLS64_02470"
     CDS             11665..12693
                     /locus_tag="PLS64_02470"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRL42730.1"
                     /translation="MNTKELIFELCCAMGVSGREEPALSVAEKFLSDYTDDIKISNGS
                     LMAVCGNKNSDKTILIDAHIDRIGFMVTEINENGFVKASPCGGIDARTLADTLLCLQN
                     NPDVTGVVCCMPPHLSDGKEDKAMPIDKIWVDFGLPYDEVNKLISFGDMLTFASKPAN
                     LLNGRIVAPCLDNRCGVASLVKCAELLKDNETDYKVIILLSSQEETFGTGAKTGAFTV
                     EADESIVVDVSFASQPDVSGFYSSVELDKGPMICISSILNKEMSKSLESVAKNKNIPY
                     QLEPIAGRTGTNADHISISGKGVKTAVVSIPQRYMHTQNEVISVADVDNTAKLIAEYI
                     KCGGAFND"
     gene            12686..13735
                     /locus_tag="PLS64_02475"
     CDS             12686..13735
                     /locus_tag="PLS64_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HRL42731.1"
                     /translation="MIDIKLLEQLCLCNGISGDEGNVRNLIINEIKDCADDIKTDNLG
                     NLIVFKKGKKSAKSKLMVSAHMDEVGLMVTDITSDGYLKFDEVGGIDRRVLPGKRVCV
                     GRNGLNGVIGVKPIHLTNGDEKTAVPKMNDMYIDIGAESREDALKYVNYGDGINFEGD
                     FKINGKTVVTKALDDRFGCYVLINLIKSEPEYDMYFTFVVQEEVGLRGARPAAFTVNP
                     DFALVIESTTAADVAEVDASHQVCNLSKGATVTVMDRATVYDKEMISAAFELAEKNNI
                     AVQYKRAVAGGNDSGAIHQSRGGVRTLAVSLPSRYIHAPSTAANLDDCESVLKLAKVL
                     SDYIAGGNLAKGKTV"
     gene            13736..14509
                     /locus_tag="PLS64_02480"
     CDS             13736..14509
                     /locus_tag="PLS64_02480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL42732.1"
                     /translation="MIVTANENYINVIMNLSAEDPFACRIISQYNSYDSSLAFVDYWT
                     IIDDKTDECTGAISRCGTSFILFLTDKTNLEEVSSFMRVAGASSAICSGDFNLNLYGS
                     KSVSGVILKKSEPFENVDESINFDVPDIKEVYNLIVSCADENFVPPPFDDFYVDVNHK
                     LRHNATRMYGVYDGTKLVAMAMTVAESSNGAVLGAVSCHPDYRKHGYGSTVVKYISNR
                     LIAENKTVYLHRAKNANQSFYNNLGFEQCGSWQEYYFER"
     gene            14515..15774
                     /locus_tag="PLS64_02485"
     CDS             14515..15774
                     /locus_tag="PLS64_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006356643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HRL42733.1"
                     /translation="MSFFDIDERVIKASEKAMELCKDKLAEIDDIQEYNQQKMIKAFQ
                     LADVRESHLWGSTGYGYDDAGREALDKVYAYVFDAEDALVRHNFVSGTHALTVALFGV
                     LRPGDTMLSITGMPYDTIRSAIGIEGDYPGSLKDFSINFEMTELNPDGTLDYDKIASD
                     IAEKKPKMVYIQRSRGYSTRPTLTWREIKKLCEISKANSPESIIMLDNCYGEFIDKVE
                     PLSVGVDMMAGSLIKNPGGGIAPTGGYIAGKKELVEMCAYRLTTPGTGKEIGATLNAN
                     RELFMGAYHAPHVTGEALKTAVFASALFEILGYETSPKYNDARGDIIQLIMLGNAEKV
                     CRFCEGVQSGSAVDSFVSPIPSDMPGYESQVVMAAGAFTLGSSIELSADAPLREPYAV
                     WMQGSLNFHSGKLGVMLAASKILRDEK"
BASE COUNT         4908 a         2982 c         3394 g         4673 t
ORIGIN      
        1 tcggcaagtt cggcaaaaag ggcagtatga tttctgctgt tgaccttctc aaaggcatcg
       61 caatttgtgc cggtatgaag agtgttgatg ttgagggtgc aacaggctat attgacacaa
      121 actttgacgg aaagtgcaaa gccgcaattg atgaattcaa aaacggtgcg gactttgtat
      181 atatccatgt tgaagctccc gatgaatgcg gtcacagagg agagattgaa aataaagtta
      241 aggctattga actcattgat gagtatattc tcaagcctgt aaccgatttt ctcagaactt
      301 ttgatgattt tgccgttctc gtatgtcccg atcatcccac acctctttct atccgcacac
      361 acacttcaaa tcctgttcct tatcttatct atgacagcaa aaacgaagtt gacagcggcg
      421 taagcaaatt ctgtgaagaa gaggctaaaa agacaggcaa ctatattgaa aaaggtttca
      481 caatgatgga ttacttcctt tctaaataat ttttgctctg tactacataa taaaccaaaa
      541 aaataacatc cttgcagata taatgaaatt aaaaatctgt aaggatgttt ttatgttgtg
      601 tttgttcatt atcataacaa taatgagtgt gattaccttg tttgtaattg cgctttctta
      661 tgcagtaaaa ttccttatcg gaataataat taaacttata cgcaaaaacg atttagataa
      721 gttttaatgc ttcaacaaga gcctttgaaa gctgatcggg gcaggatgtg cctctgccac
      781 ggcagtcaat gccttttaat ttgtcaacag cgtcttttgc gtccatacct ttaacaagca
      841 tacaaatgcc ctgagtgttg cctgaacagc cgcccacaaa ctcacagctt gtgattttac
      901 cgttgtcatc aagttcaaga ttaatctgtc tggagcaaac tcccgtaggg aaatatgaat
      961 atttcattgt aatacctctt ttcataattt gaaatcatat tatcacagaa acagaaaaaa
     1021 atccagataa ttttattttt ttgtttttaa aattgaaaat ctatgttata atttaaatac
     1081 agattgcact ttgttacaaa ccttgctccc tgttatgaaa gcagagcgtt gttttaattg
     1141 aaatccaagt gcagatgttc cacagctctg atagaatgga actattacaa atattgtaac
     1201 agacatttac aaaaagcaaa tcacagaaag taggatataa tgtcaattaa tatagatttg
     1261 ggagcatgga actcaatttt tgcagttccc tccaaagtag tcgatgaagg attaaaattt
     1321 tctgacggcg taaagcttaa agtccttttg tttgtccttc gtaatgccgg tcgttccgtt
     1381 gacgacactg aaatttcaaa ggcaacaggt gtcaatgtaa ccgatattcc cgaggcgctt
     1441 gactattggg taagtatggg tgtgtttaat cgaattgaaa atacctttac tcccgtaaaa
     1501 aataacgagg aatttgtttc tgcaccgcag acttctgttc aaacggaaca gacaccgcaa
     1561 cctgttcaga ctgttttaca gccacaacag acagtgccac agcctgttca agcggttcaa
     1621 gagcctgtaa aacaggaaac agaacagtca ccaaaggaaa acgcagaaga accgcaaaaa
     1681 cattttacgg ctacaagacc gcaaaagcct gattttgtat ttacggcaca aaggcttgcg
     1741 gttgatgaag aattaaaaat gcttgttgaa gaaacgcaga caacactcgg aaaggttctt
     1801 tcaaattccg atattgcaac tctgcttatg ctcaaggaca cctgcggact tccgcttgat
     1861 gttatcttta tgcttataca ttattgtgca agcattgata agggcaatat cagaacgatt
     1921 gaaaatatcg gtattcaatg ggcaaatgac ggtgtttatt cgcttgaggc ggctgataac
     1981 aagattaagc agattcaaaa aacaacggca aacttttcaa ttgtttcaaa agcattcgga
     2041 cttaaaaatg tcggttctcc tacaaagaaa cagcttgaat acggtgacaa gtgggtaagc
     2101 gagtggaagt tttcgcccga aatgctcaga gaggcgtatg aaagatgcgt tgatacaaag
     2161 ggaacaatga atcttcgcta cattgacgga atcttaaagc gttggaatgc aagcaatctg
     2221 cacacgcttg acgaacttca taagtatgaa aaatcagcgt caaagccgtc gcagaaacag
     2281 tcgtcatctt atgatattaa tgaactcgac aaatttaatt cacttgataa tttttaatta
     2341 agaggttggt atgggataca gcaatagtgt ttataagact gcttcggaca cattacatga
     2401 acgcagactt aacgccgaaa aggcagcgga aagaagaaaa gaagaggttt ataaaaaatt
     2461 cccccgtgtg aaagaacttg aaaaacagat ttccgtgggc ggaatcaaaa cagcccgagc
     2521 agttcttgcg ggcggagatg tgaaggacgc cgtttcaaag cttcgtgatc agaatcttgc
     2581 aatgcaggca gaggtcagga aaatccttac agataacggt tatcccgaaa attattttga
     2641 acccgattat ttttgtaaga aatgtaatga caagggctat tgtgacataa acggcaaaac
     2701 tgtcagatgt tcatgcatga agtctgctct catagcctgt gcatgtgccg agcttaacag
     2761 aaacgctccg ctgtcacttt ctacatttga gagctttaat cttgaatatt atgacaagac
     2821 tgttgatcca aagcttaagg tatctcctta cgaccatatg agcagagttt taagatattg
     2881 tcagaattat gccgacaact ttaactcaca ttcggaaagt atacttatga aaggcgcaac
     2941 cggactcggc aaaactcatt tgtcgcttgc aattgcaaac gaggcaataa agaggggata
     3001 cggtgtaatt tatgcgtctg ctccgcaact tgtttcaaag cttgaaaaaa tatatttttc
     3061 aaacaaggaa gatgattcaa catttgatat gcttgttgaa tgcgaccttc ttattataga
     3121 tgacctcgga acggaattcc attctcaatt ttccgtatca cagctttaca acatttttaa
     3181 ctcaaggatg ttgtcaaaca agcccgtaat tataaataca aatctgacaa taagagattt
     3241 gcaggagaat tatactgaca gatttgtttc aagaatctgc ggaaatgccc gtcagcttga
     3301 ttttttgggc agagatataa ggatcagacg aaaataattt taaaatattt tcgtcaaaaa
     3361 tcctgttgct gtcggctgtt ttgaaggtct gaaaactttt ttgaaaaaat tttaaaaaaa
     3421 gtattgactt tttgaacgat atgtcttata attcatatcg ttgcaagttc ggtagcaatt
     3481 acatcttgtg gaatagtgta acggtagcac gacagactct gactctgttt gttggggttc
     3541 gaatccctat tccacagcca cagccccgaa gttattcggg gctcttttat ttcgaggtgt
     3601 agcgcagttt ggtagcgcac atcgttcggg acgatggggt cgcaagttcg aatcttgtca
     3661 cctcgaccag tttggcgccg tactttttta agttcggcgc attttctttt ttaaaaaatt
     3721 tggaggtaat tttatgaaaa aagtaatttc gcttgcagtt gctcttgttc tttgcctttc
     3781 gatatttgca ggttgcggtg caaaggaagt taatcttgct gatcttatgg ataagatgaa
     3841 ttccgagtac agcgtagatg caacaaagta tgaaacaaag gatgatatgt acaaatatta
     3901 caatatcaat gccgacgata tcaagcagtt tgccgctgag gtcggtaagt ctgatacaga
     3961 cagcgaaaat acagaggtag ttcttgttga ggcaactgat tcagatgccg cttcaagagt
     4021 tgaaactgct cttacaaacc gctataattc aatctttcag cagtacgctt cctattcggc
     4081 tgaaaagctt gatatggtga aaaattgtaa ggttacaaaa gacggtaact ttgttacaat
     4141 gattatcggc gaaaaggcat ctgatatgct taaaatgttt aatgacagca ttaaataatt
     4201 tatttaaaat ttactgaggg cgaacatcgt tcgccccttt cttttgcaat attttaaggc
     4261 aggttaaaaa tggtacagac tgagattcaa aaaaactttt cacatatgaa tgatatgcag
     4321 aaacaggctg ttttctgcac agaaggtcct ttgctcatac ttgcaggtgc cggttcgggc
     4381 aaaacaactg ttcttgttaa cagaatagcc tacattttgc agtgtgagct ttgtaaacca
     4441 tggcagatac ttgccattac ttttaccaat aaagccgcag gagagcttaa agaacgaatc
     4501 tgtgcggctg ttcccgaggg cggtgccgac atctgggcgg ctacctttca ttcaacctgt
     4561 gcaagaatcc ttcgcagata cggcgacaga atcggttata caagtcattt cacggtttac
     4621 gggactgacg atcagaaaaa actcgtcaag gatattttaa agcaactcaa tattgccgaa
     4681 aaaactttgc ctgtaaaatc aattctctcc gaaatttcaa aggctaagga taaaatgctt
     4741 actcccgaag aaatgcgtaa ggaggccgaa tttgacagca gaaaagcaca gattgcaaag
     4801 gtttatgaaa tctatcagac aaagcttaaa acagccgacg caatggattt tgacgatatg
     4861 ctttgcaata ccgtaaagct ttttgaaaca gcacccgata tacttgatta ttacagaaat
     4921 cagttcaagt acatcatggt cgatgagtat caggatacta acaaggtgca gtataagttt
     4981 gtaagcctgc ttgcaagcaa atacggaaac atctgcgttg tcggtgatga tgaccagagt
     5041 atctacaaat tccgtggtgc tacaatcgaa aatattcttt cttttgaaaa tacatttaag
     5101 aacgcaaaga tgattcgcct tgaacagaat tacagaagta cacagaatat ccttaatgcc
     5161 gctaacgagg tcatctcaaa taacacaatg cgtaagggta aaactttgtg gaccgaaaac
     5221 ggtcagggcg agaaaattaa ggtacataca gccgagaatg agcgtgacga ggctaatttc
     5281 attgctcaga ctattcttga cggtgtagcc gacggaagaa aatattccga ctatgcaatt
     5341 ctttacagaa tgaatgccca gtcaaacgca attgagcagg ctttgtcacg aagcggtgtt
     5401 ccgcaccgtg taatcggcgg tcacagattc tatgaccgtg aagaaattcg tgatatggtt
     5461 gcataccttc aggttataaa taatcctcat gatgatgtca gactttcaag aattatcaat
     5521 gttcctaaaa ggggaatagg tgcgacaacc gtttcccatg ccgcagacat tgccgcagga
     5581 ctcggcgaaa gcatttacga tgtaatcaag gaggcggaaa actatccgca gctttcccgt
     5641 gcctctgcaa agctcaaagc ttttgttgca cttattgacg gacttattga agccgagcag
     5701 agcggcgaat attcccttgc agaattatat aaccttgtga ttgaacacac atcatacgaa
     5761 cagtatctta aaaccgaaaa ggacaaccct gaagttcgta ttgaaaacat cgaagaactt
     5821 tcttcaaata tcgtcaagtt tgaagaggac tacggcgatg aggcagacct ctctgcattt
     5881 cttgaagaaa tttctctcca gaccgatatt gataactacg atgcagatgc agatacctgc
     5941 gtaatgatga cattacattc ggcaaaggga cttgaatttc ctgttgtgtt catcgcaggc
     6001 atggaagaag gaatgttccc gtccgttgca acaatgatga atcccgatga actcaacgag
     6061 gaaaggcgac ttgcctatgt aggaattaca agagcaaagg agattctcta ttttacgaaa
     6121 acaaattcga gaatgctgtt cggatcaaca agctataata agggctcaag attcttaaac
     6181 gagattcccg ataatcttct tgaatacagc ggcggaagaa agcagatgtt cacacaggcg
     6241 gcttccgggt ttgcatcggg aacaggtgaa aaagtttccg tcggcaaatc gtcgggaagc
     6301 tcgtattcac cgaataaatc attcggattt acaaaacctc ctgttaagag cggtgctgta
     6361 ttcaatgtag gagattgtgt tcagcacaag gtgtttggca agggtatggt aatgaaagcc
     6421 gagaaaatgg gcaatgatac tatgcttgaa attgcctttg acaaagccgg cacaaaaact
     6481 cttatggcga actattgcag taatatgaaa aaaatctgag gtgattaaag tgcttatatc
     6541 atcaaaaaag gttctgtgtc ttgcaatttc tgcgttgaca gctttttctg tgtgccttac
     6601 aggttgcgga aaagacacgc agtcttctgt atctgctacc gagaaaacaa ctgccgtggt
     6661 tgcttcacag cctgtaacaa aggcggcaac ctccgatgag cagacacaag aacccacatg
     6721 gaactattcc gaactcaaaa gaaaatcttc cgatatttcc gaaaatcttg acgcaataat
     6781 aaccgataac aaattcaggg gaacggttta tgtcaaaatc ggaaatgctc ttgagtatat
     6841 cggcactaac ggattttccg acaaggacag gcacacaaag aattctacgg acacatgctt
     6901 tagaattgct tcgctcacaa agcagtttac tgctgttgcg gttatgcagc ttgttgagga
     6961 gggaaagctt tcacttgatg atacaatcga caaatatttt ccgtcatata aatacggcaa
     7021 aaaaatcacc gttaaaaacc tgcttacaat gacagcgggc ataaaggatt acatcaacaa
     7081 agacggcgat acaaatactt atgcatacaa cgaatctcag cttgaatata aggtttcagc
     7141 cgaaaattcc gcaaagaaaa ataaaaaagc cgtaatggac tggatacttg atcaggaact
     7201 taattttgaa cccgatgaga agtatatgta ctcaaacagt gcttattttc ttctcggtga
     7261 tatcattgaa caggtcagcg aaacctcgta cgaaaaatat attaaagaga atattcttaa
     7321 accgtcggga atgatgaaca caggctttga gggcacagat aagctcgctg taggatatca
     7381 ggatatttac gataatgcgt ggacattgta tcccggagta ggatattctg caactggttt
     7441 aatttccaat gttcccgatt tactcaaatg ggttgacgct ctttgtacaa acaagctcat
     7501 ttcggaaaaa tcttttaaag aaatgacaac gccgtacaag ggtaactacg gttacggctt
     7561 tgttgtgtca aaagacagca atatgatttc tcacacagga aaaatcgaca agtataatgc
     7621 cgctctcgct tttacaaaag acgaaaatca aatttatata gcactttcaa attattcaaa
     7681 cagcagtccg attaatctgt ttaataacat tcagaaaaca cttgccccgt tttacggtta
     7741 attttcggct gttaatcacc aaaaatcaaa acctccataa aatgtattgt tacagtgcat
     7801 taattgaact gtcaatactt ttacggaggt tatttttatg tcagatgtac ctttctgttc
     7861 ctcggaaaat gcatgcaccg aaggcggcga gcaaactaac ggttgtgaac ctgtttgtat
     7921 caatgtttca cgaatctatg acagttgcgg agcaaaagat tgcctttcaa atcttcctgt
     7981 tatgtttacc gaagcaaacc agaaaattat tgatggggct tgttccgtaa agataagtaa
     8041 tgtgcgcata attacatcta cggtagaggt tgagcctgtt gcgttccatc gtggctttta
     8101 ttcggttgat atgatgtact attttgccgt caccgttgaa gcctatacag ccgcaggtgc
     8161 gattcccgaa actgtcacag gtcttgcaat gtacggcaaa agagttgtgc tttacggcgg
     8221 tgaaggctgc gtaaagtctt tctccagtga caaagatgtt gtttgcgata catcagattc
     8281 ggattgtccg tgcaaatatc cctattgcct gccgagagca acggttcaaa tttcaaatcc
     8341 aatggcattg tctgcaaatc tacaggatta caataatgcg aatccgataa ctgtttgcag
     8401 aacatttcca agttgtgtta ttgattacct tgacggagaa cctgcacagc ctgaaacaca
     8461 gcgagtgctt gttacaatcg gtatttttac gattacaagg cttgaaagag atgtacagat
     8521 tatgattccg tcctatgatt tttgtgttcc ccgcaaggaa tgtccgtcag cgcccgatga
     8581 tccgtgcgaa gttttcagta aaattgattt tcctacagac tcattcttcc cgccgaacgc
     8641 aatacaccgt ggcgaccgtg actcttctca tacatcttct tttaaatgcg gttgtgaaaa
     8701 ctgattatac agaaaaacgg ggctgatttc agtcccgttt tgttatgcta ttttgaaatt
     8761 gtttcataaa gtaattttat tccgctttca atttttacat cttttattcc cgaaaagctt
     8821 aacacaatca tattttcggc tttgttgaac tgcatagttt taattaatgc gtcgtcaatt
     8881 ttctttataa cagaatcgtc aatcgtttta tccttaaaaa tcagcgaaac ataaagcgaa
     8941 gtttcgttaa acacaatttt aacggaggtt ccgaaatatt tctttatgtt tgaaagcata
     9001 aggttgcttt tttcaagata aattcttctt gcttttctca gatgaacatc aagcttgccg
     9061 ttattaatat actttgcaag ggcaatttgt tctgtttttg acgctgtctg atttgtgtat
     9121 tttttaattt tgttgtattc atctgtgagt ttttgcggaa gaaccatata actgattctg
     9181 acagagggaa gaagtatctt tgaaaaagag ccgagataaa ccgtgttttc ggtatcataa
     9241 ttctgtacgc acggcatagg gtgcgtactg tatcgaagct caccgttgta atcgtcttca
     9301 ataatcaaaa cattattttc ttttgcccat ttgattatct caagtctgcg tgtaacaggc
     9361 attgtaatgt tgctgtttac ggtaaaatta ggatttataa gaattatatc tggcttgatt
     9421 tcttcaagtg aatcaataca ggctccgtat ttatcacatc tgaaatattt gatgtcataa
     9481 ccgtgactgt tgaacacata ttcactctga atgtagctgt aatccgcaag tgcgattttc
     9541 ttattattgc cgagaagtga acagagtatg tgcaaaagcg tctgagtacc tgctccgacg
     9601 ataatatttt cgtatgaggt gtttacactc cgtgtgccga ggctgtattt ttgcagagca
     9661 tttctcagaa tttcttcacc ctgttcttca ccgtaggaag tgagcagata gttttgattt
     9721 aaaacttctt taacatattt tttccattcg tttatgttga ttataccgtc atcaatgctt
     9781 ttactgctga aatcatattc atagtatctt tttgttttgt tggtatttga agacatttta
     9841 tcgctttttg acggtgtacc gctgaactgt gcctcaacat aatatccgct tcgtggcctg
     9901 tttgatatat atccctctgc acacaactgt tcatacgctc cgttgacagt agtttttgaa
     9961 acatcaaggt cggtactcaa tcgccttatt gacggaagct ttgttccttt tttcagactt
    10021 cccgtttcaa ttgattttct gaccgacata taaatctgcc tgtaaagcgg agttttatct
    10081 tttttattga taattataaa atcgtacagc ataacatcac ctaccgtatt ttaacatttt
    10141 gaaaatatta ttgcaatatc agtgtattac ggttgaaatt atttccgtta aaatgtataa
    10201 ttgtatggta tggcataaag tcactgaaat ggcataaaat actaattgca gaggggattt
    10261 tacaatgaat gctatggctg ataaattttt tgacagtata aaagggaaaa aagttacttt
    10321 tatcggaatc ggtacaagca atcttccgct tataaaactt tttgttgaaa agggtgcaaa
    10381 agttactgct tgcgataaga agaattttga agatttggga cagaacggta ttaaggctaa
    10441 agagtacggt gcagaacttt tactcggtga aaattatctt tccgagattg atgccgatat
    10501 cgttttcaga agtcccggca caccttttta taaggatgag cttgttgcac ttcgtaacaa
    10561 gggcgttgtt cttacatccg aaatggaagt tttcttcgac ctttgcccgt gcaggattat
    10621 tgctgtgaca ggttctgacg gcaaaacaac cacaacaacg attatttcgg aatttttaaa
    10681 ggcggcaggt tttaatgtac accttggagg taatatcggc aagccgcttc ttcctgagat
    10741 tgaaagcgtg aatgacgatg actgtgcagt agttgagctt tcaagttttc agcttatctc
    10801 tatgagaaag agtccggata ttgccgtggt aacaaacctt gctcccaacc atcttgatat
    10861 acacaaggat atgcaggagt acattgattc aaagaaaaat atcattcttc atcagaatgc
    10921 tttttcaaag gctgttctca accttgataa tgagattaca aacggctttt ccgacagcgt
    10981 aagaggtcag ctcgcaaagt tctccgtaaa agaaaatgtt tcaaacggtg cttttcttga
    11041 cggcgataca atctgctata atgactacgg aaaaattacc cgtattatga acattcatga
    11101 catcaaaatt cccggtatgc ataatgtaga aaattatctt gccgctattt cagccgtatg
    11161 gggacttgtt gatgcagaaa caatgacagc tgttgcaaag acttttggcg gtgttgctca
    11221 ccgtgcagag tttgtaagag aggttgacgg tgttaagtat tacaacgatt caattgcgtc
    11281 aagtcctaca agaaccgcac tcggcacact ttcgctttac agccgaaaaa tttgcattat
    11341 tgcaggcgga tatgataagc atattccgta tgagccgctc ggacccgtaa tcaacgataa
    11401 agtaaaactc cttattcttc tcggtgatac tgccccgaaa attgaaaaag ccgttaaaga
    11461 ggcttcaaac tatgatgcag atgaaattga aatcgtcaat gtaacaaata tggaagaggc
    11521 tgtagctgtt gcccgtgagc gtacgacaaa gggagacatt gtttcgcttt cacccgcaag
    11581 tgcaagcttc ggactttaca agaattttga agaaagaggt aatcacttca agtcaattgt
    11641 caacgctttg aagtaagata tacaatgaac acaaaagaac ttatttttga actctgttgt
    11701 gcaatgggtg tgtcgggcag ggaagaaccc gctttgtctg ttgccgaaaa gtttttatcg
    11761 gattacacgg atgacataaa gatatcaaac ggcagtctta tggctgtttg cggtaacaaa
    11821 aattcagata aaaccatatt gattgacgct cacattgacc gcataggatt tatggttacc
    11881 gaaataaatg aaaacggatt tgtaaaagct tctccgtgcg gaggaattga cgcaagaact
    11941 cttgccgata ctctgctttg tctgcagaac aatcctgatg taacaggtgt tgtatgctgt
    12001 atgcctcctc atctttccga cggaaaagag gacaaggcta tgccaatcga taaaatctgg
    12061 gttgatttcg gtctgccgta tgatgaagtt aataaactca tttcattcgg tgatatgctg
    12121 acatttgcct caaagccggc aaatcttttg aacggcagaa ttgttgcgcc ttgccttgat
    12181 aacagatgcg gtgttgcatc acttgttaag tgtgcagagt tattgaaaga caatgaaaca
    12241 gattataaag taattatcct tttaagctct caggaagaaa catttggcac aggcgctaaa
    12301 acaggtgctt ttacagttga agcagacgag tcaattgttg ttgatgtaag ctttgcatct
    12361 cagccggatg tttcagggtt ctattcttcg gttgaactcg acaaaggacc aatgatttgc
    12421 atttcttcaa ttctcaacaa agaaatgagc aaatcgcttg aaagcgttgc aaagaataaa
    12481 aatataccgt atcagcttga accgatagca ggcagaacag gtacaaatgc cgaccatatt
    12541 tcaatcagcg gaaaaggtgt aaaaactgct gttgtatcaa tcccacagcg atatatgcat
    12601 acacagaatg aagttatttc tgtagctgat gttgacaata cggcaaagct tattgccgaa
    12661 tacattaaat gcggaggtgc tttcaatgat tgacataaaa ttgcttgaac agctttgcct
    12721 ttgcaacggt atttcgggtg atgaggggaa tgttcgcaat cttataatta acgagattaa
    12781 agactgtgcc gatgatatta aaacagataa tctcggcaac cttattgtgt ttaaaaaggg
    12841 aaaaaagtct gcaaaaagca aacttatggt gtcggcacat atggatgaag taggtcttat
    12901 ggtaacggat ataacatctg acggttacct taaatttgac gaggtcggag gaattgacag
    12961 aagagttctt cccggtaagc gtgtatgtgt tggcagaaac ggtcttaacg gtgtaatcgg
    13021 tgtgaaaccg attcatctta caaacggtga tgaaaaaact gctgttccga aaatgaatga
    13081 tatgtacatt gatatcggtg ccgaaagcag ggaagatgcg cttaaatatg taaactacgg
    13141 tgacggcatt aattttgagg gcgattttaa gattaacggc aaaactgttg ttacaaaggc
    13201 acttgacgac cgtttcggct gctatgtgct tattaatctt ataaaatccg aacccgaata
    13261 tgatatgtat ttcacatttg ttgttcagga agaagtggga ttgcgtggtg caagacctgc
    13321 ggcttttacg gtaaatcctg attttgctct tgttatcgaa agcacaactg ccgctgatgt
    13381 tgcagaggtt gatgcatctc atcaggtatg caatttgtca aaaggcgcaa cggttaccgt
    13441 aatggatagg gcgacagttt atgataaaga aatgataagt gccgcttttg aacttgccga
    13501 aaagaataac atagctgttc agtataagag agcagttgca ggcggtaatg attcgggtgc
    13561 aattcatcag agcagaggcg gagtgagaac tcttgcggta tcacttccgt caaggtatat
    13621 tcatgctcct tcaactgcgg caaaccttga tgattgcgaa agcgttttaa aacttgcaaa
    13681 agtattgtcc gattatattg cgggcggaaa tttagcgaaa ggaaagacgg tctgaatgat
    13741 agtaaccgcc aatgaaaatt atataaatgt aattatgaat ctgtcagcgg aagatccgtt
    13801 tgcgtgcaga ataatttctc agtacaacag ttacgattca tcgcttgctt tcgttgacta
    13861 ctggacgata attgacgata aaacagatga gtgcacaggt gcaatttccc gatgcggaac
    13921 aagctttatt ttatttctta cagataaaac aaatcttgaa gaggtttcgt catttatgcg
    13981 tgttgcaggt gcgtcaagtg caatatgcag cggagatttt aaccttaatt tatacggcag
    14041 taaatccgtt tcgggcgtta ttctgaaaaa atcagaaccg tttgaaaatg ttgacgaaag
    14101 tataaatttt gatgttcccg atattaaaga ggtttataat ctcatcgtaa gctgtgccga
    14161 tgaaaatttt gttccgccgc cgtttgatga tttttatgtt gatgtgaatc acaagcttcg
    14221 tcataatgca acgagaatgt acggtgttta tgacggcaca aaacttgttg caatggctat
    14281 gaccgttgcc gaaagcagta acggtgccgt gttgggtgcg gtatcgtgtc atcccgacta
    14341 cagaaagcac ggctacggtt caactgttgt aaagtacata tccaacagac tgattgccga
    14401 gaacaagacc gtgtatcttc acagagccaa gaatgcaaat cagtcgtttt ataataatct
    14461 cggttttgaa cagtgcggat catggcagga atattatttt gaaaggtgat atatttgagt
    14521 ttttttgata tagatgaaag agttataaaa gcatccgaaa aggcaatgga gctttgcaag
    14581 gacaagcttg cagagattga cgatattcag gaatacaacc agcagaagat gataaaggct
    14641 tttcagcttg cagatgttcg tgaaagccac ctttggggtt ctacaggcta cggatatgat
    14701 gatgcaggca gagaggctct tgacaaggtc tatgcctatg tttttgatgc agaggacgca
    14761 cttgtccgtc ataatttcgt aagcggtact catgccctta cagttgcact tttcggcgtt
    14821 ttaagacctg gggatacaat gctttccata acaggaatgc cgtatgatac aatccgttct
    14881 gctataggca ttgagggtga ctatcccggt tcactcaagg acttttcaat caattttgaa
    14941 atgaccgaac ttaatcctga cggcacactt gactatgaca aaatcgcaag cgacattgca
    15001 gaaaaaaagc ctaaaatggt ttacatacag cgttcaagag gctacagcac aagacctacg
    15061 ctcacatgga gagaaatcaa aaagctttgt gaaatttcaa aagccaattc gcccgaaagt
    15121 attattatgc ttgataactg ttacggtgaa tttattgaca aggttgaacc tttgtcggtt
    15181 ggtgttgata tgatggcagg ctctcttatc aagaatccgg gcggcggtat tgctcctaca
    15241 ggcggatata ttgcaggtaa gaaagaactt gtagagatgt gtgcctacag acttaccact
    15301 cccggtacag gcaaagaaat cggtgctact cttaacgcaa acagagaact ttttatgggt
    15361 gcgtaccatg ctcctcatgt tacgggggaa gcacttaaaa ctgcagtatt tgcatcggca
    15421 ctttttgaaa ttctcggtta tgaaacaagt cctaagtaca atgatgcaag aggagatatc
    15481 attcagctta tcatgctcgg taatgcagaa aaggtatgcc gcttttgcga gggtgttcag
    15541 agcggttcgg ctgttgacag ctttgtatct ccgattccgt cggatatgcc gggttacgaa
    15601 agtcaggtag ttatggcggc aggcgcattt actctcggtt catctattga actttctgca
    15661 gacgcaccgt tgcgtgaacc gtatgcagta tggatgcagg gaagtcttaa cttccacagc
    15721 ggtaagctcg gtgtaatgct tgcggcaagt aagattttaa gagatgaaaa ataatataat
    15781 cttgttgctt tgattataac agcaaataaa aaatcccgct tttgtaagcg ggatttttgc
    15841 tatgattttt tattgcgtaa gtttttaaaa aagtcggtga gaagttttga acactcctcc
    15901 tgcataatac cgccgactga ttcgggtttg aaattgaacg gcatttggaa cagattc
//
LOCUS       DAOMVE010000024        15832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_756_length_15832_cov_3.689358, whole genome shotgun sequence.
ACCESSION   DAOMVE010000024 DAOMVE010000000
VERSION     DAOMVE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15832
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_15832_cov_3.689358"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /gene="glgB"
                     /locus_tag="PLS64_02490"
     CDS             <1..1815
                     /gene="glgB"
                     /locus_tag="PLS64_02490"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HRL42734.1"
                     /translation="RKYEDGNVFSYKKLAEELIPYVKEMGYTHIEFMPLTEYPFDGSW
                     GYQVTGYFAATSRYGEPKDLMYFVDRCHEEGIGVILDWVPAHFPKDAHGLGRFDGTGC
                     YEYEDPRIGEHKEWGTYIFNYGRYEVTSFLLSSAMFWLDKYHVDGIRVDAVASMLYLD
                     YNRKDGEWIANAYGGRENLVAVDFLQKLNTVVHMFHPEAMMIAEESTAWPNVTRYPIK
                     DMGLGFDYKWNMGWMNDMLSYISLDPLWRNYHHDTLTFSMVYAFSENFMLPISHDEVV
                     YGKGSLINKMPGDYDMKFSGVRGFIAYMMAHPGKKLMFMGSEIGQFDEWNANEELEWN
                     LLSFEKHRQLNLFFAEVNKFYRDVPAMHENDYDWDGFQWIALDDYKNSIISFRRIAYD
                     GSEIICVCNFQPVQHDNYVVGVPEYGIYEEVFNSEAEEFGGCGIRNEGELKAKKKKDH
                     ELNYSMEITVPPLAVMYFKLKKKLALPVKKKTTAKKDAALAKLKAEKAKKSAKAAPKK
                     EDKAEKVKVEAKSAKAEATAEKVEKKAEPAPEKPVKKAVKAVKEETPAPAEVEKKAEK
                     AVKVEKAEKVEKTETAVKAESKTVKAEKPAEKKTTAKK"
     gene            2029..3747
                     /locus_tag="PLS64_02495"
     CDS             2029..3747
                     /locus_tag="PLS64_02495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRL42735.1"
                     /translation="MKIDNKKARIILAVIIAVSLVISTVYMFAKQGFHEDELLTYNLA
                     NSSKQLNVDGGWNSPEDFNEYLAVSPDDRFDYAQVYENQIIDASHPPLYYALVHTVCS
                     FFPGVFSKWLAYSINVLAMAGILIMLYKIGRKITGNNLYALIGVGAYALSIACITTTI
                     YLRMYATLTFWVLAFVYMSIKLYEKKNTVGIKDCILLAVTVLCGVLTQYYFILFAGLT
                     GLVFLVFKIKEKCGKDLIKYIIAAVVGAGLALCIYPYIISNVLGGNRGLSSLDMSALD
                     MITVVTYVFYKLCTYVQILAKDMFINQVWLLGLCTAAVIGFGIYFRFVKKVKIPKKAM
                     FAVVPAVCYFIGISLVSPFNSDRYVMASLPLIAMLFAFSFIRIFTLFKNQKIRLAVPV
                     CILLASVTAFVTVKPYYTYGKTNLYEPKTDNCIFVGTAMLEWNKCIDKFMNYDSTMIV
                     QSSKFSPTLGDELEEFAEKRGVITNGKVSSLAEAFMFNGDTNKQETDSMEKLKTDKKL
                     ASLDEVTVYVSRLADKDDVLKYITENTKFKNYELIQADYSFEDFYNWYDYFVETESYC
                     NVYRFY"
     gene            3762..4670
                     /locus_tag="PLS64_02500"
     CDS             3762..4670
                     /locus_tag="PLS64_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRL42736.1"
                     /translation="MNSDKIDRKNSIGVAFSGGGLQGIAHIGAVQALYELGIHPQYVS
                     GTSSGAAMAAIVAMGCDSDEMKRVAKKHWKTLAEIDNGLIFKSLAQLILNKKIQKDGI
                     KSGELIEDVIRDIMNEKGITGFENLPINLSICTVDTLTTDECIFTTKEENLENEHIHY
                     ITGAPLETAVRASMSFPAIYTTCPYDKYNFIDGGSKDNLPVKILRDMGVGKVLAIGFD
                     IMTYNPDAGLGGLIKVIWRALDVYSIDGTRESKKMADLAIDIKNENTQLFSMDSVDET
                     IQEGYDAIMAHKDEILKVFGEQNSAE"
     gene            4745..5380
                     /locus_tag="PLS64_02505"
     CDS             4745..5380
                     /locus_tag="PLS64_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42737.1"
                     /translation="MNKTRKVSGKMSVPKQIMTTFFALLFGGGMGIVAKFLDLNRLPS
                     FLDWLDLSNFLGRTAPWIFIAVCLAVFSKSPLKAGINVFAFFVGMLIGYYVWTSVFAG
                     FYPDIPYLMRWLILAVVSPFLAFLIWYARGRGALAIGISSVLIAIFCCFAFNLNFADF
                     RILYFPEFILWLASIGILFKDVKQSAFSLLISIPVALSLEYTGLLGIIGIG"
     gene            5501..5680
                     /locus_tag="PLS64_02510"
     CDS             5501..5680
                     /locus_tag="PLS64_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRL42738.1"
                     /translation="MDISVGDRVEMKKQHPCGCKTFEILRVGMDFKIKCEKCGREVMA
                     PRAKIEKNIKKILTE"
     gene            5688..6761
                     /gene="prfA"
                     /locus_tag="PLS64_02515"
     CDS             5688..6761
                     /gene="prfA"
                     /locus_tag="PLS64_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRL42739.1"
                     /translation="MFEKIEIFDKRYSELSERLYQPSVAGNPEEYQKIMKEIKSIEEI
                     VLTYRKYKEAVQTEKDSLEILEETSDKELKELAQIELDEAKDNIKNLAEDLKILLLPK
                     DPNDERNVIVEIRGGAGGEESALFSAVLFRMYSMYAEKKGFKIEIINANETEIGGYKE
                     ISFMIEGEGAYSRFKYESGVHRVQRVPETESQGRVHTSTTTVAVLPEAEDVELEIDPN
                     DLKIDTFRSSGAGGQHINKTSSAIRITHLPTGMVVECQDERSQYKNKDKALKVLKSRL
                     LKEKQDKQASEIAENRKMQVGTGDRSERIRTYNYPQGRVTDHRIGLTLYKLEEILNGS
                     LDEVIDALVAADRAEKLKESMEK"
     gene            6775..7809
                     /locus_tag="PLS64_02520"
     CDS             6775..7809
                     /locus_tag="PLS64_02520"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRL42740.1"
                     /translation="MKTLLLKNTEEDIKTAAELLKNGGVVGIPTETVYGLAADALNPE
                     AVKKIFKAKGRPGDNPLIVHICDFSDIERLNLVTKIPDTAKKLADAFWPGPLTMIMKK
                     SDVIPNEVSAGLDTVAIRFPSHKTAQAIIRESGTVLAAPSANISGSPSPTTAQHVMND
                     MDGKIDAVLDGGASEVGLESTVITLAGDVPRVLRPGGITVEMLESVLGKVEVDDAVLH
                     KLADNVKVASPGMKYKHYAPRARVILLNCNDEQYIDYVNKKAAEGVAALCCDEDIPLL
                     KTPVFSLGKRNDYTRQAHTLFDELRRIDEDDSIKIVYSRLPKTNGVGMAVYNRLIRAA
                     GFEVIDLEQN"
     gene            7796..8407
                     /gene="coaE"
                     /locus_tag="PLS64_02525"
     CDS             7796..8407
                     /gene="coaE"
                     /locus_tag="PLS64_02525"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRL42741.1"
                     /translation="MNRIKIVGLTGQSGAGKSTVSAYFAKNGMCVINADEIVKNLYTP
                     DSVCLKAVSGVFGQDIILSDGNIDRPLLAKRAFSSKENTHLLNSIVHPFVTYEFMQMA
                     KQAQTDGKSVVIYDAPQLFESGADVFCDLIVSVTAKKEIRLERICKRDNIDISRAELR
                     MSAQLDEEFFRSHSDIVIENNFSIEQVELAAQKAAEAINNLMR"
     gene            8463..9032
                     /locus_tag="PLS64_02530"
     CDS             8463..9032
                     /locus_tag="PLS64_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HRL42742.1"
                     /translation="MAWIIVLAILAAGVFVFVSVFYEDAKKKLDEMNYPRTYSTYVEK
                     AAKDYDLDPALIYAVIHTESGFDPKAESGVGAKGVMQMMPSSFEWLQEQRGCAGQYTE
                     DDLFDPEICIDYGSYLLKYFYDYYGTERSAIAAYNAGFVVSDWLNDTNYSSDGKNLDS
                     IPYPETENYVDKVESAKEMYIKLYYSQSN"
     gene            9119..10531
                     /locus_tag="PLS64_02535"
     CDS             9119..10531
                     /locus_tag="PLS64_02535"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRL42743.1"
                     /translation="MAEEKSKTTLLKEKIMMTEPKGVKALSEEEIKKADSFCDGYKKF
                     LDNSPVEREAVRYTVELAKKAGFAEYEQDKEYKAGDRLYYVNRDKAVALAVIGKNGVK
                     NGARLAIAHIDSPRVDLKPNPLFEANNLAFFKTHYYGGLKKYQWTTIPLSLHGSVVKL
                     DGTRVDICWGDDENESCFCITDLLPHLAREQASKPMAKAIEGENLNVILGSRPYDADS
                     DEKDLVKLNVMAVLNEKYGICEGDFLSAELCLIPSFKSRDIGFDRSMIGGYGHDDKVC
                     AYPAVMAALDVEMPEQTCITYLTDKEETGSDGNTGMQSDFLRFFIYDLAKQDGVDGYR
                     VLSKSTCLSADVNAAFDPTYASAYEANNCSYINNGVIISKYTGHGGKYDTSDASAEYM
                     GKIRAMLENNDILWQVGELGKVDGGGGGTIAKYVANMNVDVVDLGVPVLSMHAPFEIV
                     SKTDVYMAYRAFFTFFDTKD"
     gene            10615..10691
                     /locus_tag="PLS64_02540"
     tRNA            10615..10691
                     /locus_tag="PLS64_02540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10649..10651,aa:Arg,seq:acg)
     gene            10941..11297
                     /locus_tag="PLS64_02545"
     CDS             10941..11297
                     /locus_tag="PLS64_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRL42744.1"
                     /translation="MSESILRNKSKEFAKQIVYMCRDIKANARESVLTNQLLRSGTSI
                     GANIHEAQYAQGKKDFISKLEIAQKECFETEYWLELLFETGYISETVYKPLQNQCGTI
                     RRMLISSINTVKKNDK"
     gene            11601..12812
                     /locus_tag="PLS64_02550"
     CDS             11601..12812
                     /locus_tag="PLS64_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRL42745.1"
                     /translation="MRNVLKSVFSKKVSAAVLTAVMLFVSVFSMSMIASAASFSPRLS
                     APSSSNKYYYSNLNVFYKYGYGMPNCTAYAYGRAYEILGSEPKLSWNNAEQWYDYNKS
                     NGYYKYGQTPKVGAIACWSYNGGGGHVAVVEKVENGQITFSNSAWSGKRFYLSYASTS
                     DKNAGGNSWWNFQGYIYLGDFSGSTSTTQATTKKVTYTTGTYKTDVDDYLNMRSGAGT
                     SYSYVTSVPGNVTLNVTKVSTAGGYTWGYTSYNGKTGWVALDFCTFVSSSTVQPTTAK
                     PTVQPTTAKATVQPTTAKATVQPTTAKATQPATTVPAATVQPTTVQATTAPVTTQPSN
                     DNNNDTPYMIGDVNLDGHVNVIDATEISKYIVSISTLSDVQRELADYNHDGEINVVDA
                     TEIQKLVVGNN"
     gene            complement(12931..14244)
                     /locus_tag="PLS64_02555"
     CDS             complement(12931..14244)
                     /locus_tag="PLS64_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL42746.1"
                     /translation="MDAFIKFLQAFFSFVIIIPLSLFCLLPLKNQLKYPISKIVTLFL
                     CSFIILGSLSSIVMTAFDIQNLNFVLFPDLVIFFFLIKSVTKAGTARCLFVFISVCCL
                     ISFFSLYSYFINSFFQEEISRGVNTSYSLIQMGLSVAAMGALVPLTKYYAWMIDNINI
                     GKVWYLFSILPIALMMSTIYTIPISYANIRVGKVYAKGFIITIFELALYLIVFILFYI
                     FSKTTIEKTKLTERNNILEIQKSYTESLQSYIKYTSKARHDFKHSVHVMSRLADEGNL
                     SALKDYIAQYENSLTVTAPVRICKSDALNALFNHYRQQAIENNVDINWRIDLPDKSRI
                     LDVDLCSIFGNILENAIDGCCTVEEGKRYFNLTSEIKGDCLYIVSTNNYGKPLEMDGE
                     TYSSTKHQGKGIGLHSMKSITEVYDGIFDAGNNNGEFFVDIMLKY"
     gene            complement(14247..14978)
                     /locus_tag="PLS64_02560"
     CDS             complement(14247..14978)
                     /locus_tag="PLS64_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRL42747.1"
                     /translation="MKIAVIDDEKPQADILYSICNAYFGGHKQNVQIDCFPDGIAFFN
                     SFKENTYKIAFVDIFMEKMNGIAVAEKLREKDRDILIVFVTTSPDFMMQAFSVHAFHY
                     ITKPYKAEQIYKVLDDASSYVSDNSKYIEVMCDRRNTLISLKEIVSVESDAHYINIGV
                     VSGLRYRTRMTITEFLKLADNDTRFIPVNRGIVLNADYIKDIEDSVCVTGNGERFPIK
                     IREKTRIEQSIREYMFKKIRENQRR"
     gene            15228..15731
                     /locus_tag="PLS64_02565"
     CDS             15228..15731
                     /locus_tag="PLS64_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42748.1"
                     /translation="MKKIKKLALLLAICMVGTVGLVGCGNDGDSSSKNESSASQTADD
                     NANSADDSAADSKDGDSSASVVGTWTVTALEGTDGTAVSIDDYATANGVDPETLKCTY
                     TFADDGTFSGEMSGVTVSGNYTFEGTDLVMTVGEQQFNYTYDADNDMLKYTDPNTGLS
                     SYCERQQ"
BASE COUNT         4767 a         3096 c         3585 g         4384 t
ORIGIN      
        1 agaaaatacg aggacggcaa tgtattcagc tataaaaagc tcgccgagga gcttattcct
       61 tatgtaaaag aaatgggcta cactcacatt gagtttatgc ctcttacaga atatccgttt
      121 gacggcagct ggggatatca ggttacaggc tactttgccg caacatcccg ttacggtgag
      181 ccgaaagacc ttatgtattt tgtggacaga tgtcatgaag agggcatcgg cgttatcctt
      241 gactgggttc ctgcccactt cccgaaggac gcacacggtc ttggcagatt tgacggtaca
      301 ggctgttatg aatatgaaga tccccgtatc ggtgaacaca aggaatgggg tacatatatc
      361 ttcaattacg gcagatatga ggtaacaagc ttcctccttt catctgcaat gttctggctt
      421 gacaagtatc atgttgacgg tatccgtgtt gacgccgttg catcaatgct ttatcttgac
      481 tacaacagaa aagacggcga gtggattgca aacgcatacg gcggaagaga aaaccttgtt
      541 gccgttgatt tcctgcagaa gctcaacacg gttgtacata tgttccaccc cgaggctatg
      601 atgattgccg aagaaagtac ggcatggccg aatgttacac gctatccgat taaggatatg
      661 ggactcggct ttgactacaa gtggaatatg ggttggatga acgatatgct ttcgtacatc
      721 tcacttgatc cgttgtggag aaattatcac cacgacactc ttacattcag catggtttat
      781 gctttttccg agaactttat gctcccgatt tctcatgatg aagttgttta cggcaagggt
      841 tctctcatca ataaaatgcc cggcgactat gacatgaagt tcagcggtgt gagaggtttc
      901 atagcgtata tgatggctca cccgggtaag aagcttatgt tcatgggcag tgaaatcggt
      961 cagtttgacg agtggaatgc gaacgaggag cttgaatgga accttctttc atttgaaaag
     1021 caccgtcagc ttaacctttt ctttgctgag gtaaacaagt tctatcgtga tgttcctgcc
     1081 atgcatgaga acgactatga ttgggacggt ttccagtgga ttgcacttga tgactacaag
     1141 aacagcatca tttcgttccg cagaattgct tatgacggca gtgaaatcat ctgcgtatgt
     1201 aacttccagc ctgttcagca cgataactat gttgttggtg tgcccgaata cggtatttat
     1261 gaagaagtgt tcaactccga ggctgaagaa ttcggcggtt gcggaatcag aaacgagggc
     1321 gagctcaagg ctaaaaagaa gaaagaccac gagctcaact attcaatgga aatcacagtt
     1381 ccgccgcttg ctgttatgta tttcaagctt aaaaagaagc tggctcttcc cgtaaagaaa
     1441 aagactacgg caaagaagga cgccgctctt gcaaagctta aagccgaaaa ggcaaagaaa
     1501 tcggcaaagg ctgctccgaa aaaggaagat aaggcagaaa aagtaaaggt tgaagcaaag
     1561 tctgcaaagg cagaagcaac cgctgaaaag gttgaaaaga aagccgaacc cgctcccgaa
     1621 aagcccgtta aaaaggcagt aaaagcagtt aaggaggaaa ctcctgctcc tgctgaggtt
     1681 gagaaaaaag cagaaaaagc agttaaggtc gaaaaggctg aaaaagtcga gaaaaccgaa
     1741 actgccgtaa aggctgaaag caaaaccgta aaagctgaaa agcctgccga aaagaaaaca
     1801 acagctaaaa aataatcaaa atattacaaa tcatttgtgc cgacggattt ttctgtcggc
     1861 atttttgttt gcccgaaaac gcattacata caaaattttt gtaaagtcta ttgttattgt
     1921 gaccgtattt gtggtataat catttaaata cggaatgccg aaaattgtcg tttgaacggc
     1981 acaaaattgt cggctgattt taattttgat tattacggag aattttatat gaaaatcgac
     2041 aacaaaaagg caagaataat tcttgcggtt attattgcgg tgagccttgt cataagcacg
     2101 gtttatatgt ttgcaaaaca gggctttcac gaggacgagc ttctgaccta taaccttgca
     2161 aattcctcaa aacagcttaa tgttgacggc ggttggaaca gtcccgagga tttcaacgag
     2221 tatcttgccg tgtcgcccga tgacagattc gactatgctc aggtatatga aaatcagatt
     2281 atagatgcgt cgcatccgcc tctctattac gcacttgttc acacggtctg ttcattcttt
     2341 ccgggcgtgt tctcaaaatg gcttgcctat tcaataaatg tgcttgccat ggcaggcatt
     2401 ttgattatgc tttataaaat cggacggaaa atcacgggca acaatcttta cgccttgatc
     2461 ggtgtggggg catatgctct gagtattgcg tgtattacca cgacaattta tctcagaatg
     2521 tacgcaacgc ttaccttctg ggtgcttgcg ttcgtttata tgagcataaa gctctatgaa
     2581 aaaaagaaca ccgtcggcat aaaggactgc attctgcttg cagttacagt gctttgcggt
     2641 gtgcttaccc agtattattt catcctcttt gcaggactta cgggacttgt tttccttgtg
     2701 ttcaaaatca aggaaaagtg cggtaaagac cttattaagt acattattgc ggctgttgtg
     2761 ggggcaggac ttgcactctg catttatccg tacataatct cgaatgtact cggcggcaac
     2821 cgtggactca gttcgcttga tatgtcggcg cttgatatga ttacggttgt gacctatgtt
     2881 ttctacaagc tttgcaccta tgttcagatt cttgcaaagg atatgttcat caatcaggtt
     2941 tggctgctcg gactttgtac agccgcagtt atcggattcg gaatttattt ccgttttgtg
     3001 aaaaaggtaa aaattcctaa aaaggcgatg tttgccgtag ttccggcagt ttgctatttc
     3061 atcggcattt cacttgtttc accgtttaac agcgaccgct atgttatggc gtcacttccg
     3121 ctgattgcga tgctttttgc attctcgttc atcagaattt tcacactttt caaaaatcag
     3181 aagatacgcc ttgccgtgcc tgtatgcatt ttgcttgcgt cagtcacagc gtttgttacg
     3241 gttaagccct attacacata cggcaaaaca aatctctatg agccaaagac agacaactgc
     3301 atttttgtcg gaactgcaat gcttgaatgg aacaagtgta ttgacaagtt tatgaactac
     3361 gacagcacaa tgattgttca gagttcaaag ttctctccga ctctcggtga tgagcttgaa
     3421 gaatttgccg aaaaacgagg agttatcacc aacggaaagg tttcttcact agccgaggca
     3481 tttatgttta acggcgacac aaataagcag gaaaccgaca gcatggaaaa actcaaaacg
     3541 gacaaaaagc ttgcgtcact tgacgaggta acggtttatg tttcccgcct tgcagacaaa
     3601 gatgatgtgc ttaaatacat aacggaaaac acaaaattta agaattatga gcttattcag
     3661 gctgattaca gctttgaaga tttctacaac tggtacgact attttgttga aaccgagtcg
     3721 tattgcaatg tttatcgttt ttattaacag aggtgtttaa aatgaattcg gataaaatcg
     3781 atcgcaaaaa ttctatcggt gtagcatttt cgggcggcgg acttcaaggt attgctcaca
     3841 tcggtgctgt tcaggctctt tatgaactcg gaatccatcc gcagtatgtt tcgggtacaa
     3901 gctcgggtgc ggcaatggcg gcaatcgttg caatgggctg tgattccgat gaaatgaaaa
     3961 gagttgcaaa aaagcattgg aaaactcttg ccgaaatcga caacggcctt attttcaaat
     4021 ctcttgcaca gcttattcta aataaaaaga ttcagaaaga cggcatcaag tcgggtgaac
     4081 tgattgaaga tgttatccgt gacattatga atgaaaaagg aataacaggc tttgaaaatc
     4141 ttccgattaa cctttcaatc tgtacggttg acacgcttac aaccgatgaa tgtattttta
     4201 caaccaagga agaaaatctt gaaaacgagc atattcacta cataacaggc gctccgcttg
     4261 aaactgctgt tcgtgcaagt atgtcgttcc ctgcaattta cacaacctgt ccttatgaca
     4321 agtacaactt tattgacggc ggttcaaagg acaatttgcc cgtaaaaatt ctgcgtgata
     4381 tgggcgtcgg caaggttctt gcaatcggct ttgatattat gacctacaat cccgacgcag
     4441 gccttggcgg tcttattaag gttatttggc gtgcgcttga tgtttattca attgacggca
     4501 caagagagtc aaagaaaatg gctgaccttg caattgatat caagaacgaa aacacacagc
     4561 ttttctcaat ggacagcgtt gacgaaacca ttcaggaggg ctacgatgcg attatggctc
     4621 acaaggacga aattttaaag gttttcggtg agcaaaacag tgcggaataa ttaagattaa
     4681 agaggacttc ttttttgaga agtccttttg atgtagatga aacgaggatt ttatgttaaa
     4741 aattttgaat aaaacccgaa aggtatcggg caaaatgtcc gtaccaaaac aaattatgac
     4801 aacattcttt gctctgcttt ttggcggtgg catgggaatt gtcgcaaagt ttcttgattt
     4861 gaacagactg ccgagctttc tcgattggct tgatttgtca aactttttgg gcagaacagc
     4921 accgtggatt tttattgccg tgtgccttgc cgtattcagc aaatcaccct taaaagcagg
     4981 aattaatgtt tttgcgtttt ttgtgggaat gttaataggg tactatgtgt ggacaagtgt
     5041 ttttgcaggc ttttatcccg atattccgta ccttatgagg tggctgattc ttgctgttgt
     5101 atcgccgttt ttggcatttc tgatttggta tgcaagggga cggggtgcgc ttgcaatcgg
     5161 aatttcttcc gtactcatag caatattctg ttgctttgca tttaatctca actttgccga
     5221 tttcagaatt ctgtacttcc ccgaatttat actttggctt gcaagcatcg gaattttgtt
     5281 caaggatgta aaacagagcg cattttctct gctgatttca attcccgttg ccctttcgct
     5341 tgaatatacg ggacttctcg gaattatcgg cattggttga tatttttaaa tctctgttgt
     5401 ataatctaag tgcagattat ttgcatctct aaagtgatgt aaataatcat agaaattcaa
     5461 aatgtgaaaa atgtaaaaaa tttgcaggag ggtgattttt gtggatatca gcgtcggcga
     5521 cagagttgag atgaaaaaac agcacccatg cggttgcaaa acctttgaaa ttctgcgtgt
     5581 gggaatggat tttaagataa aatgcgaaaa atgcggcaga gaggttatgg cgccccgtgc
     5641 aaagattgaa aaaaatatta aaaaaatttt gacggagtaa gatatatatg tttgaaaaaa
     5701 tcgaaatatt tgacaaaagg tactccgagc tttccgaaag gctctaccag ccgtcagtag
     5761 cgggcaatcc cgaggaatat cagaaaatta tgaaggaaat caagtcgatt gaggaaatcg
     5821 ttttgaccta ccgtaaatac aaagaggctg ttcaaaccga aaaagacagc cttgaaattc
     5881 ttgaggaaac aagcgacaaa gagcttaaag agcttgctca gatcgagctt gacgaggcta
     5941 aggataatat caaaaatctt gcggaagatt tgaaaattct gcttttgccg aaggatccga
     6001 acgatgaacg gaatgttatc gttgaaatcc gtggcggtgc aggcggtgag gagtcggctc
     6061 tgttcagtgc ggttctgttc agaatgtact cgatgtatgc cgaaaaaaag ggctttaaaa
     6121 ttgagattat caatgccaat gaaaccgaaa tcggcggtta caaggaaatt tcctttatga
     6181 ttgagggcga gggtgcatac tcacgcttta agtacgaaag cggtgttcac cgtgttcagc
     6241 gagtacccga aaccgaaagt cagggcagag ttcacacatc gacaacaacc gttgcggttt
     6301 tgcccgaggc agaagatgtt gagcttgaaa ttgatccgaa tgacctcaaa attgacacct
     6361 tccgttcaag cggtgcaggc ggtcagcata tcaacaaaac aagttccgca atccgaatca
     6421 ctcacttgcc cacaggtatg gtagttgagt gtcaggacga gagaagtcag tacaagaaca
     6481 aggacaaggc tcttaaagtt ctcaaaagcc gacttctcaa ggaaaaacag gacaaacagg
     6541 cgagtgaaat tgccgaaaac agaaaaatgc aggtaggtac gggcgaccgc agtgagcgaa
     6601 tccgcacata taactatccg cagggcagag taaccgacca cagaatcggt cttactctct
     6661 acaagcttga agaaattctc aacggcagcc ttgacgaagt gattgacgca cttgttgccg
     6721 ctgacagagc cgaaaagctc aaagaaagta tggaaaaata atccggtgat aattgtgaaa
     6781 acattgttgt taaaaaatac agaggaagat ataaaaaccg ccgcagagtt gcttaaaaac
     6841 ggcggtgttg taggaatccc gaccgaaaca gtttacggac ttgcggctga cgcactcaac
     6901 cccgaggccg taaagaagat ttttaaagca aagggcagac ccggggataa tccgctgatt
     6961 gtccacatct gcgatttttc cgatattgaa agacttaacc ttgtgacgaa aatccccgac
     7021 acggcaaaaa aacttgccga tgcgttctgg cccggaccgc tcacaatgat tatgaaaaag
     7081 tcggatgtta ttccgaatga agtttcggca ggacttgaca cggttgcaat ccgttttccg
     7141 agccacaaaa ccgcacaggc gattatccgt gaatcgggta cggttcttgc cgcaccttcg
     7201 gcaaatattt cgggctcacc cagtccgaca accgcacagc atgttatgaa cgatatggac
     7261 ggcaaaatcg acgctgttct tgacggcggt gcaagtgagg taggtcttga aagcacggta
     7321 attactcttg cgggagatgt tccgagggta cttcgcccgg gcggaattac ggttgaaatg
     7381 ctcgagagtg ttctcggcaa ggttgaagtt gatgatgcgg ttttgcacaa acttgcagac
     7441 aatgttaagg ttgcaagccc cggtatgaag tacaagcact atgcgccaag ggcgagagta
     7501 atacttctta attgcaacga tgagcaatat attgattatg taaacaaaaa ggcggctgaa
     7561 ggggttgcgg cactctgttg cgacgaggat attccgcttt tgaaaactcc cgttttctct
     7621 ctcggaaaac gaaacgacta tacaagacag gctcacacgc tttttgacga gcttcgcaga
     7681 attgatgagg acgacagtat taagattgtc tattcaagac tgccaaaaac gaatggcgtc
     7741 ggaatggctg tttacaaccg gcttataaga gcggcaggct ttgaggtgat tgaccttgaa
     7801 cagaattaaa attgtcggtc ttacgggaca gagcggtgcg ggaaaaagca cggtttcggc
     7861 atattttgcc aaaaacggaa tgtgcgttat aaatgctgac gagattgtaa aaaatcttta
     7921 cactcccgat tccgtatgct taaaggctgt ttcgggtgtt ttcggtcagg atattatcct
     7981 ttctgacggc aacattgaca gaccgttgct tgccaaaagg gcattttcaa gcaaagaaaa
     8041 cacacatttg ctcaattcaa ttgttcatcc gtttgttaca tatgaattta tgcagatggc
     8101 gaaacaggct cagacagacg gcaaaagcgt tgtgatttat gacgctcctc agctttttga
     8161 aagcggtgcc gatgtgtttt gcgaccttat tgtaagcgtt accgcaaaaa aggaaataag
     8221 gcttgagcga atctgcaaaa gggataacat tgacatttcc cgtgcagagc taagaatgtc
     8281 tgcacagctt gacgaggaat ttttcagaag tcacagcgac attgtcattg aaaacaattt
     8341 ttctatagag caagtcgagc ttgccgcaca aaaggcggca gaggctatta acaacctgat
     8401 gaggtgatag gatgccccaa tcaaggcgat ataaaaagaa aaaaagtgca ggaaaggcga
     8461 ttttggcatg gataattgtg cttgcaattc ttgcggcggg agtatttgtg tttgtttcgg
     8521 tcttttacga ggacgcaaag aaaaagcttg atgagatgaa ttatccacgc acctacagca
     8581 catatgttga aaaagccgcc aaggattatg accttgatcc tgcgttgata tatgccgtaa
     8641 tccacacgga aagcggcttt gacccgaaag ccgaatcggg agtcggagcg aagggtgtta
     8701 tgcagatgat gccatcgtcc tttgagtggc tgcaggagca aagaggttgt gcgggacagt
     8761 acaccgagga tgacctgttt gaccccgaaa tctgtattga ttacggaagt tatcttttaa
     8821 aatattttta cgattattac ggcacggaac gctcggcaat tgccgcatat aatgccggct
     8881 ttgttgtaag cgactggctc aacgatacta attacagttc ggacggaaaa aatcttgatt
     8941 ccattccgta tcccgaaaca gagaattatg ttgacaaggt tgaaagtgcc aaggaaatgt
     9001 atataaaact ttattattca caatctaatt aagtgcaaag atgttccgat gcctttggcg
     9061 gcggatttca aaataaaatg gttttgccgt gttttacggc acagaaagga cggttattat
     9121 ggctgaagaa aagtcaaaga caactttatt aaaggaaaaa attatgatga ccgaaccgaa
     9181 gggcgttaag gctctttcgg aggaagaaat taaaaaggca gacagctttt gcgatggtta
     9241 caaaaagttt cttgataact cacccgttga gcgtgaggct gttcgctata ctgttgagct
     9301 tgccaaaaaa gcaggttttg cagagtatga gcaggacaaa gaatataagg ccggtgacag
     9361 gctttattat gtaaaccgtg acaaggctgt tgctcttgcc gtaatcggta aaaacggtgt
     9421 taagaacggt gcaaggcttg caatcgctca tattgattct ccgagagttg acctcaaacc
     9481 gaatcctctt tttgaggcaa acaatcttgc tttctttaag actcattact acggcggtct
     9541 taaaaaatat cagtggacaa caattccgct ttcacttcac ggttcggttg taaagcttga
     9601 cggtacaaga gttgacatct gctggggcga tgatgagaac gaaagctgtt tctgcattac
     9661 agaccttctt ccgcaccttg caagagagca ggcgtcaaag ccaatggcaa aggcaattga
     9721 gggcgaaaac ctcaatgtta tcctcggctc tcgtccgtat gatgcagaca gcgacgaaaa
     9781 agaccttgta aagctcaatg taatggctgt actcaatgag aagtacggta tctgtgaggg
     9841 tgacttcctt tcggctgagc tctgccttat cccgtctttt aagtcaagag atatcggttt
     9901 tgacagaagc atgattggcg gttacggtca cgatgataag gtttgtgctt atccggctgt
     9961 tatggcggct cttgatgttg aaatgcccga gcagacttgt atcacatatc ttacagacaa
    10021 agaagaaaca ggcagtgacg gcaacacagg tatgcagagc gactttctcc gtttcttcat
    10081 ctatgacctt gcaaagcagg acggtgttga cggctaccgt gttctttcaa aaagcacctg
    10141 tctttctgcc gatgtaaatg ccgcatttga tccgacttat gcgtctgcat atgaggctaa
    10201 caactgctca tacattaaca acggtgtaat tatttcaaaa tacacaggtc acggcggaaa
    10261 gtatgacaca tcagatgcgt ctgccgaata tatgggaaaa atcagagcta tgcttgagaa
    10321 caacgatatt ctctggcagg ttggtgaact cggcaaggtt gacggcggcg gcggcggaac
    10381 aatcgcaaag tatgttgcca atatgaatgt cgatgttgtt gatctcggcg ttcccgtgct
    10441 ttcaatgcat gctccgtttg aaattgtttc caagacagat gtttatatgg catacagagc
    10501 atttttcact ttttttgata caaaagatta atttcggaaa aaatacaaaa aacacttgat
    10561 ttttgcaccg agttgtgata tcatactact tgcggctgat aaaaagccgc atatgcgccg
    10621 gtagctcagc tggatagagt gtctgactac gaatcagaag gtcgggagtt cgagtctctt
    10681 ccggcgcgcc aagaaaaacg cacttgtgta aacaagtgcg tttttcagtt atattcgcct
    10741 gtcggcgagt gatattgccg tgcagtgata ttcggtcata ccgagtgata ttgcctgcgg
    10801 cagttttaaa gcgaatataa tatcaccgag agcgaaagcg agcgataaca catttatatc
    10861 acgccgagtt aaacgaggca tatcaccgat aactttacag acattcggct ttgtgatata
    10921 ttgtattttg ggtgatgatt gtgtctgaaa gtattttacg gaataaatca aaagaatttg
    10981 caaaacagat tgtatatatg tgtcgtgata tcaaagcaaa tgcaagagaa tcggttttaa
    11041 ctaatcaact tttgcgttcg ggaacaagta taggtgcaaa tattcacgaa gcacaatatg
    11101 cgcagggtaa aaaggatttt atctccaagt tggaaatagc acaaaaagag tgctttgaaa
    11161 ctgaatattg gctggaactt ctttttgaaa caggatatat atccgaaacc gtatataagc
    11221 cattgcaaaa tcagtgcggc acaatcagga gaatgctgat ttcatcgatc aatacagtaa
    11281 agaaaaacga taaatgaaac atataaacat tctaacgccg taagcgtttc tgatgattaa
    11341 gtttatctaa tcctttacac cttaaaactc agacctcatc cttgtacaat aaacactatc
    11401 tattaaagaa tgttagttaa attttgtgca aagtatgcat aaaatgaaaa ttattgtgta
    11461 caatggctta aaaaaataga ttataaaggc tattttcagc atttctactc tttgaatttg
    11521 gagcaatttg tgatagaatg tacataaagt ggttacaaat aggtaacaat cactttgaat
    11581 tcaaaaaaag gagtgtttct ttgcgaaatg ttttaaaaag tgtttttagc aaaaaggttt
    11641 ctgcggcagt tctcacggct gtaatgctgt ttgtgtcggt tttctcaatg tcgatgattg
    11701 cgagtgcggc atcgttctca ccgagactgt ctgccccgag ttcaagtaac aaatattatt
    11761 acagtaattt aaatgttttt tataagtatg gttacggcat gcctaactgt acagcatatg
    11821 cctacggcag agcttatgag attctcggtt ctgagccaaa gctttcatgg aacaatgccg
    11881 agcagtggta cgattacaac aagtctaacg gctactacaa gtacggtcag actccaaagg
    11941 tcggcgcaat tgcctgctgg tcatataacg gtggcggcgg tcatgttgct gttgtagaaa
    12001 aggttgaaaa cggtcagatt acattttcta actctgcatg gagcggtaag agattctacc
    12061 tttcctatgc aagcacatcc gacaagaatg ccggcggtaa cagctggtgg aattttcagg
    12121 gctacattta ccttggcgat ttcagcggca gtacaagcac tactcaggct acaaccaaaa
    12181 aggtaactta cacaacaggt acatataaaa cagatgttga cgattatctc aacatgagaa
    12241 gcggtgcggg tacaagctat tcatatgtaa catctgttcc gggcaatgtt actcttaatg
    12301 taacaaaggt ttcaacagcc ggcggttaca catggggtta cacctcgtac aacggcaaga
    12361 caggttgggt tgctcttgat ttctgtactt ttgtttcttc atcaacggta cagccaacaa
    12421 cagcaaaacc tactgtacag cctaccacag ccaaagccac ggttcagccg actactgcaa
    12481 aggcaactgt tcagccgaca acagccaagg ctacacagcc tgcaacaact gttcctgctg
    12541 caacagtaca gcctactacg gtacaggcaa caacagctcc tgtaactaca cagccgagca
    12601 atgacaacaa caatgatact ccgtatatga tcggcgatgt aaaccttgac ggtcatgtca
    12661 atgttattga cgcaactgaa atcagcaagt acattgtttc aatttcaaca ctttctgatg
    12721 ttcagagaga acttgcggat tacaaccatg acggtgaaat caatgtcgtg gatgccacag
    12781 aaatccagaa acttgttgtc ggtaacaact gataacaaca aaacaaaatc aaaacacgct
    12841 ttgcaaaacg cagagcgtgt ttttttgttt ggaactttta taacaaagaa gggtatgcga
    12901 ggatgcatac cctttttgag tttaataagt ttaatattta agcattatgt caacgaaaaa
    12961 ttcaccgtta ttgttgcctg catcaaaaat tccgtcatag acttcggtta tgctcttcat
    13021 ggagtgaagt ccgatgccct tgccctgatg ctttgtggac gaataagttt cgccgtccat
    13081 ttccaaaggc ttgccgtagt tgtttgttga aacaatgtac aaacagtcgc cttttatttc
    13141 ggaagtcagg ttaaaatatc ttttgccttc ttctacggta cagcaaccgt caattgcgtt
    13201 ttcaagtatg ttgccgaaaa tactgcagag gtcaacatca agaattctcg atttgtcggg
    13261 caaatcaatc cgccagttaa tgtcaacatt gttctcaatc gcctgttgac gatagtgatt
    13321 gaacaaagca ttgagtgcgt cacttttgca gattcgcaca ggtgctgtta cagtcagcga
    13381 attttcatac tgggcaatgt agtctttcag cgccgaaaga tttccttcat ctgcaagtcg
    13441 gctcataaca tgaactgaat gtttaaaatc atgccttgct tttgaggtgt attttatgta
    13501 cgactgcaac gattctgtgt aacttttctg aatttcaaga atgttgtttc gttctgtaag
    13561 ctttgttttt tcaatagtag tcttgctgaa aatgtagaat aatatgaata ctataagata
    13621 caatgcaagc tcaaatattg taatgatgaa tccttttgca taaacctttc caacacgaat
    13681 atttgcgtag gaaatcggta tggtatagat ggttgacatc ataagtgcaa tgggcaaaat
    13741 tgaaaacaaa taccacacct tgccgatatt tatgttgtca atcatccatg catagtattt
    13801 tgtaagcgga acaagcgctc ccattgccgc aaccgaaagc cccatttgaa tcaggctata
    13861 tgaggtgtta actcctcgtg agatttcttc ctgaaaaaat gagttgataa aatatgaata
    13921 cagcgaaaag aatgaaatca aacagcacac acttataaaa acaaacaaac agcgtgctgt
    13981 tccggccttt gtaacgcttt taataaggaa gaaaaatatc accaggtcgg ggaagagaac
    14041 aaaatttaaa ttctgaatgt caaaagccgt catcacaatt gacgagagag aaccgagtat
    14101 gataaatgaa cacaaaaaga gcgttactat ctttgaaatc gggtatttta attgattttt
    14161 taacggtaaa aggcagaata aacttaacgg tattattatg acaaagctga aaaatgcctg
    14221 aagaaatttt atgaatgcgt ccatgttcac ctccgctggt tttctctgat ttttttgaac
    14281 atatattcac ggatactctg ttcaatccga gttttttcac gaattttaat cgggaatctt
    14341 tcaccgttgc ctgtaacaca tacagagtct tcaatgtcct ttatgtaatc ggcattcagc
    14401 acgattcccc tgtttacggg tatgaaacgg gtgtcgttgt ctgcaagctt gagaaactct
    14461 gtaatggtca ttcgtgtgcg atatctaagt cccgaaacaa cacctatgtt gatataatgt
    14521 gcatcggatt caacagaaac aatttcctta agcgaaatca gcgtgtttcg gcggtcacac
    14581 ataacttcaa tgtattttga attatccgat acatatgacg aggcatcatc aagtaccttg
    14641 taaatctgct cagctttgta gggctttgtt atgtaatgaa aagcgtgaac ggaaaatgcc
    14701 tgcatcataa aatcggggct tgttgtgaca aatacaataa gaatatcacg gtctttttca
    14761 cgaagctttt cagccacggc aatgccgttc atcttctcca taaaaatatc tacaaaggct
    14821 attttgtagg tgttttcctt gaatgaattg aaaaaagcta ttccgtcagg aaaacaatca
    14881 atctgcacat tttgcttgtg tccgccaaaa tatgcgttgc agattgagta gagaatgtct
    14941 gcctgaggct tttcgtcgtc aataactgca attttcaaga tatcgcctcc tttgaaataa
    15001 tttaattcgg agcaatgatt aactgcactt agattatacc ataaatttga aaaatattat
    15061 atccgattgt catatgtttt tgccaaaaga ttgatgtttg tgccaacttg ttgaaatgtt
    15121 gcattaaatt ggctatcatt gaggtgataa aagcgaaaca cgaaaattgc ggtgtttccg
    15181 taaatcaaaa tcggaaagta aaaaatatcg gaaagaggta atcaactatg aaaaaaatca
    15241 agaagttagc tttattgctt gcaatttgta tggtaggtac agtaggtctt gtaggttgcg
    15301 gtaatgacgg tgactcgtca agcaaaaacg aaagttcggc atcacagact gctgacgaca
    15361 atgcaaacag cgcagatgac agcgccgctg attcaaagga cggcgattca tcggcaagcg
    15421 ttgtaggtac ttggactgta acagcacttg aaggtacgga cggtacagcc gtttcaattg
    15481 atgactacgc aacagcaaac ggtgttgacc ccgaaacttt gaaatgtaca tacacatttg
    15541 cagatgacgg tacattctca ggcgaaatga gcggtgtgac tgtttcgggc aattacacat
    15601 ttgagggtac agatctcgtt atgacagtag gcgaacagca gtttaactac acatatgacg
    15661 ctgacaatga tatgctcaaa tacacagatc ctaacacagg cctttcaagt tattgcgaaa
    15721 gacagcagta attgtaatct aatcccaatt tcctaatact acaaaaacaa ccgctcactt
    15781 tcttggtggg cggttgtttg cttttgatat gcggttgtca ttctgttggg gc
//
LOCUS       DAOMVE010000025        15734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_766_length_15734_cov_3.736718, whole genome shotgun sequence.
ACCESSION   DAOMVE010000025 DAOMVE010000000
VERSION     DAOMVE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15734
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_15734_cov_3.736718"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLS64_02570"
     CDS             complement(<1..348)
                     /locus_tag="PLS64_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42749.1"
                     /translation="METTNKKNAELKELYQAFNIKKKVKFETEKYNGIQLLTCFDSYL
                     NADNKIMIYGQEANTDEGCVFDFSPQYQKDGYYKYEYKIAHVGEKGIPKSECRQTEYL
                     KTRELIACFDKSLN"
     gene            complement(406..>590)
                     /locus_tag="PLS64_02575"
     CDS             complement(406..>590)
                     /locus_tag="PLS64_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42750.1"
                     /translation="FSEETDDEITVTVNVNELAMEQFAKAFTPWIEVIKPVALRERMI
                     KNLNKSLGKYMSNIVL"
     assembly_gap    591..613
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<614..1519)
                     /locus_tag="PLS64_02580"
     CDS             complement(<614..1519)
                     /locus_tag="PLS64_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRL42751.1"
                     /translation="MIEIRGGMMFAKSPKKLIIMNILDILKRYTDENHRLSQKDIQDI
                     LEREYDMTVERKAVKRNLMNLIEFGYNINYSESVRIFKDKDGKEQENVILSDFYLEHE
                     FTDSELRLLIDSVLFSNHIPYKQDKELVDKLASLSNIYFKKRVNHIARMPEDKTDNKQ
                     LFYNVELLDEAIDKRRKVCFHYLEYHTDKKLHKRRNKNGKVREYIINPYQLVAKEGKY
                     YLICNYDKYDDISNYRIDRITDLEILDENIKPFDQLKGSDGRKLDLEEYMDKHVYMFS
                     GENVRAVFRADKSLISDIIDMFGKEV"
     gene            complement(1805..2164)
                     /locus_tag="PLS64_02585"
     CDS             complement(1805..2164)
                     /locus_tag="PLS64_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnpV protein"
                     /protein_id="HRL42752.1"
                     /translation="MTNTKITYTQQGDFLLPDLKLPEQPKVEIGIFGKRHLRYLERNR
                     KILYTNMLTKGKLTAYLADIDEQAENMFDQLVRQLAEREGVTEQLKANNQMLWVKKMN
                     SIRNRAEEIVNHELIYS"
     gene            complement(2369..2445)
                     /locus_tag="PLS64_02590"
     tRNA            complement(2369..2445)
                     /locus_tag="PLS64_02590"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2409..2411),aa:Met,seq:cat)
     gene            2797..4011
                     /locus_tag="PLS64_02595"
     CDS             2797..4011
                     /locus_tag="PLS64_02595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRL42753.1"
                     /translation="MGKNKTRSKSALKQNSVTAENKRLGDDGNINFTFKILSAIGIII
                     IVSGHCYNGGVSLMYDWFPKYSYNLALFVFISGYFYKEKHEEHIFKYIWGRTKRLLIP
                     AYLWNIVYGIIAFALTQVGYTIAQGKFDLYNLFVMPFIDGESFAYNLGSWFVYPLFCV
                     YVFNILFRKLLKKVHRGNEYVILAVYLAIGIFGVQYCIWNPAPKGALLLLFRSMFFLP
                     CFEFGRFYHEKLEKHDNLNSVAYFAIVLGVQLLLLTFCENLEYTPSKCVKFDNGCVIP
                     YVTAITGIAFWLRVAKLITPIIKNKKLVRMISDNTYSIMIHHIFAFMIVKWAFWGLSV
                     ITPLFKDFDILKLKSTIWYIYRPNGLNFYCIIYLAAGIFIPILIGTVTHRIFEFMKEH
                     TLKSMQKKQSDR"
     gene            4254..4967
                     /locus_tag="PLS64_02600"
     CDS             4254..4967
                     /locus_tag="PLS64_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42754.1"
                     /translation="MPIYRIADLNVEVNPKFKICRKRLESYAVKSNESGKTDIAISVS
                     DDEIRERLRLSDNLSEQGAEDVLILSKFCNAVLDGFDGFFFHSSCLELDGEGYVFSAV
                     SGTGKSTHTALWRRHFGNRVTMINDDKPVIRKCDGKFYAYGTPWMGKADIGTNTKAPV
                     KAVYILQRAEENTAVRVLPSQVMKQLLEATVIDVERSRMEKLLELLDEFFKETPLFLL
                     GCNMDESAVMTAYNAANGG"
     gene            5003..5863
                     /locus_tag="PLS64_02605"
     CDS             5003..5863
                     /locus_tag="PLS64_02605"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HRL42755.1"
                     /translation="MEFVQVVSVENMRKSDARTIAEHTPSAELMYRAAQGIFNSAKFV
                     GRVAIVCGKGNNGGDGYALACVLCKNGFTPTIFRASDGFSKDGLYYYKTAMSLGAEEM
                     PMSQAAAFTGFDIVVDCLLGTGFSGELKGEMLEAVEQINMTNAYVISADINSGINGDT
                     GVCSTAVNSDLTVSIGSFKTGLFLNDAPYYIGSVTNCDIGISLIEDEYKLIDYSLLHM
                     FEGYGSLVMTAEEFFEKYGYEPSKCNVARCVEEISKKERRTVVVKTDHSAVIADLKYI
                     YFCADYVINN"
     gene            5893..6726
                     /locus_tag="PLS64_02610"
     CDS             5893..6726
                     /locus_tag="PLS64_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL42756.1"
                     /translation="MKYKKVLLPPEPYYSGRVTRYPDRKVAFESGLRWHGSYEISYIK
                     KGKVRLNKLDCEIILNAGDVYVLNSQEVHSYEDIGEDTQAVMLNIIPKAIAPYVDDPH
                     IVPSFKQPEGEALEFIRKAMDIFHGYEEYESKLISMKVRAVMYSVAYYLIRDCIDNDI
                     QYVHGSQADDFDCAKSAIRYMHDNYKHEITLNEIANYVGMTPAHFSKYFKDKTDETFS
                     KYLRRVRLEHAIDDLRNKKITVRQAAIENGFPNVNSFILFCKSEYGRTPAELRNYSEK
                     N"
     gene            complement(6946..7791)
                     /locus_tag="PLS64_02615"
     CDS             complement(6946..7791)
                     /locus_tag="PLS64_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRL42757.1"
                     /translation="MKNFSIILGAIILVLFVTLVIWGVYCDNTIAITNYCVNNTKNDA
                     KLKFVVITDLHNKEYGEKNADLAELVKEQNPDFIAVCGDMVNRGDPDTTKMKDVLEKL
                     SKIAPTYCCLGNHERDNAAKFGTDFKSETNSTGAVLLDNEYIKFTKNGKSVLIGGMSD
                     YPYYEFYTPDDDVPSRTLWEEFAEKAKNNFTILLHHQPEYIAEDAKKTDIDLIVCGHT
                     HGGQIQLPFIGGVIAPNQGLFPKYDKGEFDLDGTKMIVCAGLSNTVFIPRINNQVEIG
                     VVTVV"
     gene            8032..9909
                     /locus_tag="PLS64_02620"
     CDS             8032..9909
                     /locus_tag="PLS64_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HRL42758.1"
                     /translation="MKKINLRKMFLALADVFIIVVSGILANYILLLVGYIGAASSKGL
                     LSYIVIDLVMCLFTLYISGTYSKLWRYFNAKDYIVCGTAVFSGFTLGFVISLFLQIPQ
                     RKIFCIVHFAIALVGILAFRFVFRRAFLDLTEAGRAEGGERTLIVGAGNAGRMIVREI
                     HNAKEQGDVNNPSKSIIPVCFVDDDLKKLNQKIEGIPVVGTCPEIVKICDEYRIDNII
                     VAVPSCEEDEKRKILDYCSATECKIKIIPYLGELLFDDGHTQLISQAKEIKIEDLLGR
                     KPIEFDRKEIHDLINGKVCLVTGGGGSIGSELVRQIAKNDPKQIIIVDIYENNAYDIQ
                     QELVLEYGTKLNLVTLISSVRDYDKMELIFKTYRPELVFHAAAHKHVPLMETVPEEAV
                     KNNIFGTFNVATLAEKYKAKKFVMISTDKAVNPTNVMGATKRACEMIIQYKAQHSTDT
                     EFVTTRFGNVLGSNGSVIPLFRREIENGAPVTVTHPDIIRYFMTIPEAVSLVLEAGAM
                     AKGGEIFVLDMGAPVKITTLAENLIRMYGKVPYKDVPIIFTGLRPGEKLFEELLMDEE
                     GLKSTQNKKIFIGNQIKIDETDMLSKLEVLKKTAENNDSEKTVELLAELVPTFCHKVN
                     D"
     gene            9928..11757
                     /gene="glmS"
                     /locus_tag="PLS64_02625"
     CDS             9928..11757
                     /gene="glmS"
                     /locus_tag="PLS64_02625"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRL42759.1"
                     /translation="MCGIVGYVGPKDCSEVLVNALTKLEYRGYDSAGIAVFDNGEIKT
                     VKTKGKLQDLKDKLAEVGTPKGHVGIGHTRWATHGEPSDVNSHPHSGTRVTIVHNGII
                     ENYMELKDFLISKGRTFLSDTDTEVVAQLLDYKYNGNPLETIDSVMAELKGSFALGIM
                     FKDFPDRVFAVRRESPLIVGVAEGECFIASDVPAILQYTRDYYLLAHDEIVTLSPDGV
                     SFVDEHLDPIEKELQTADWDMEAAEKGGYPHFMIKEINEQPEAIRTTIMPRIKEGLPF
                     LEECGITTETIKNFKNITIVACGTAMHAGMVGKYVIEDIARVPVNVDIASEFRYRNPI
                     VGEGDLVIIISQSGETADSLAAMRLAKQKGAKTLAIVNAKGSSIAREADMLIYTLAGP
                     EISVPSTKAYITQLSVMYLFAFELALAKETISTAECKRLVSALTKMPEAVQYVIDNCE
                     DTCKFVATKLVATDSLLYIGRGLDYALSMEGSLKLKEVSYIHSESYAAGELKHGTISL
                     IDEDVPVIAVATQTGIIPKTISNVVEVKSRGAKVILVCTDACARELKDGVADYVIEIP
                     HTDELLMPITAVVPLQILAYYTSINRGIDPDKPKNLAKSVTVE"
     gene            12305..14284
                     /locus_tag="PLS64_02630"
     CDS             12305..14284
                     /locus_tag="PLS64_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42760.1"
                     /translation="MMKTVFKRAVTLAICAVMVFCGIFACSGATKDYEIPQIDDMTLS
                     MPDGMSAVTRDSKSTDKYFSVFGLDYDTTMKTFQNGNIYLQGMDNMSSVILTVTMTKN
                     ESSENVGNYAQLSSSELSNIRNNFLNSDEYRSCTPDQKEKVVWLVFDATAQSSKGDVK
                     TFVANTVYDGMNINITMQRTEGDVTTVDYDTFSKIVSSVKFSDLPFSKGFIPYIIIGG
                     GVVILILIVLIIIFAVRIRKHKKAKKNNAILEELAVKYKLGEKGTDYDIDNFDDSPEK
                     PDKRSKKKQKYAEEVEEEPALPGRRYAPEPIIENMEEPEEITSEYSNFGYDTDDAKEK
                     EIDEIINSAKAYKSELEREAYKNNYGYEENSTEDEKKKEPTAADTSETEPEKIDENEK
                     DNAETPEKHEDISSSSGPTLVIPEPEHDLFETSEPDDIDREDDADVEETEEVSEQKHP
                     LEKKVDKYANIIFGSDDPEEKTGEEVDDEELVRAKAKKNKFDSGYDFFDEAPKKSMGV
                     IKSSDLRDAEDFDVIGEVERKAETVKQNALEKENNKETEGEPTDKRSTEEVLANVGKN
                     IGNALRGFAGGVKNFGIHCGYFCKNVSIMIKHKRAIAKRKKAEEERRERERQKAERAR
                     MRAERGESDGLVQVHSRTDRRPQQNRRPANRNKRR"
     gene            14310..15575
                     /locus_tag="PLS64_02635"
     CDS             14310..15575
                     /locus_tag="PLS64_02635"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRL42761.1"
                     /translation="MEMLIKNAVVVSPADKLNEKTDILIKDGVIAEIGSGITSDGEII
                     DADGLCAVPGFVDMHVHLRDPGQTHKEDIITGCKAAAAGGVTSLLAMPNTNPTTDSPE
                     IVKYILDKAKDADSRVYVAASVTKGLKSEEPTDIDAVKSAGAIALTDDGRPVENTKYL
                     SDAMKKAPKLGMTIVAHCEDLFLADGGKINEGEVSEKLGVKGIPAGAEDCGTAREIAL
                     AAALNEPIHICHVSTKTSVALIRDAKRRGVKVTAETAPHYVSLTDRELLRGDADYRMN
                     PPLRTQADVDEIINGLCDGTLDAIATDHAPHTPEEKADFVKAPNGSIGMETSFAVCNT
                     YLVKTGRMTLSQLVEKMSVNPARILKINAGTLSVGANADVVLINPDEEWTVDAGKLHG
                     KSKNTPFKGRTLTGKVKKTILGGKVVFED"
BASE COUNT         4522 a         3123 c         3566 g         4500 t
ORIGIN      
        1 gttaaggctt ttgtcgaagc aggctataag ctcacgcgtt tttagatatt ctgtttgcct
       61 gcattctgac ttcggaatgc ctttctcgcc gacatgagcg attttatatt catatttata
      121 ataaccgtct ttctgatact gcggtgaaaa gtcaaaaaca cagccctcgt ctgtatttgc
      181 ctcctgaccg taaatcataa ttttattatc tgcattaagg taagaatcaa agcaagtgag
      241 aagttggatg ccgttgtatt tttccgtctc aaacttaacc ttcttcttta tattaaaggc
      301 ttgatacagc tcttttaatt cggcattctt tttatttgtt gtctccaatg cctctcaaat
      361 atcctcgttt ttgaataatc catatctgtc attgtatctc acctgttata aaactatgtt
      421 tgacatatat tttcccaaac ttttatttag gtttttgatc atcctctccc gcaaagcaac
      481 tggcttgata acctcaatcc acggtgtaaa tgcctttgcg aattgctcca tagcaagctc
      541 gttgacgttt actgtcacag ttatttcgtc atcggtttct tcactgaatc nnnnnnnnnn
      601 nnnnnnnnnn nnngacttcc ttgccgaaca tatctataat gtcgctgatc aggctcttgt
      661 cagctctgaa cactgccctc acattctcgc ccgaaaacat atagacgtgc ttatccatat
      721 actcctcaag atcgagcttt ctgccgtcgc tgcccttgag ctgatcaaag ggcttgatgt
      781 tttcgtcaag gatctccaaa tccgttatgc ggtcgatgcg gtagttgctg atatcgtcat
      841 acttatcata gttgcaaatc agataatact tgccctcttt ggcaactaac tgataaggat
      901 tgatgatgta ttccctaacc ttgccgtttt tatttcgccg cttatgcagc tttttgtcag
      961 tgtgatattc aagatagtga aagcacactt tgcgtctttt gtcgatcgcc tcgtctaaca
     1021 gctcaacatt gtagaaaagc tgcttgttat ccgtcttatc ctccggcata cgggcgatat
     1081 ggttcactcg ttttttgaaa tagatattgg acagagaagc taacttatcg accagctcct
     1141 tatcctgctt ataggggata tgattcgaga acagaacgct gtcaatcagc agtcgtagct
     1201 cactgtcggt aaattcatgt tccagataaa agtcggaaag gattacattc tcctgttctt
     1261 tgccgtcctt gtctttgaat atgcgcacgc tctcgctata gttgatgtta tagccgaact
     1321 cgatcaggtt catcagattg cgcttgaccg ccttgcgctc aaccgtcata tcatattcac
     1381 gctcaagaat atcctgaata tctttctggc ttagccggtg attttcgtct gtgtatcgtt
     1441 tgaggatatc caaaatattc attattatca gttttttcgg agatttggcg aacatcatac
     1501 cacctcttat ctctatcata tcatattgaa attcattctg caataataat acactgcaag
     1561 atggtgcgtt tttagtacac ggatgtcttg tgaatctaaa gttaaacgcc ccttgataag
     1621 cgatttgcct atcaaggggc atttgattta tcttatgcgc tgataatatt gtttttgacg
     1681 agcagcttcg ggtcggcttt cagtacactg agaaaacgcc ttgccgttcc gatcggttta
     1741 ttggttcctg cttcccacgc ttcaattgtc tttaccgaaa cgccttttac ctcacgatct
     1801 gcttttagct gtaaatgagc tcgtgattaa cgatttcctc tgctcggttg cggatagaat
     1861 tcatcttctt aacccacagc atttgattgt tcgccttgag ctgttccgtt acgccctcac
     1921 gctctgcgag ctgccttacc agctgatcaa acatattctc cgcctgctcg tcgatatcgg
     1981 cgagataagc ggtcagctta cctttagtaa gcatatttgt gtagaggatt ttacggttac
     2041 gctccaaata gcgaagatgc cgtttcccaa atatgccgat ttccaccttt ggctgttcgg
     2101 gcaattttaa atcgggaaga agaaagtcgc cctgctgtgt gtaagtgatt tttgtgtttg
     2161 tcataacttt aacctccgaa aaatatttgt cggaaacgat ttcggcggct caggttttaa
     2221 tagtgactgc gatttaactg tatttattct gactcaactt ttcccaactc gattttgttg
     2281 ccgtttataa accgcataga taagccgttt tcgggcattt tcggaaaaat tggaaaaaga
     2341 aaagacggtt tcgaaaaacc gtcttgtttg gttgcgggag ccggatttga accgacgacc
     2401 ttcgggttat gagcccgacg agctaccgag ctgctctatc ccgcgatata caactctgct
     2461 gttttttaca gctttattat tatagcatta tgtaaaggtt gtgtcaatag ttttttgcat
     2521 attttttatt ttatgctttg tacggctttt ttgactgaat ttatttacat tttaatctta
     2581 gttttttaag ggtatgtaca caattgtatg gtggaacagt tgcaaatgaa agatttaaat
     2641 gcctgttgaa acaaaagtga gattgtgcaa aataatgatt tatatgtgtt tagcacggtg
     2701 aatttgatat attgattgcg gattgctttt gtggtacaat taccgtgtga atttgcggaa
     2761 tttttaaatt gcgacaaata cagatgagag ggaattatgg gaaagaacaa aacacgcagt
     2821 aaatctgcac ttaaacaaaa cagtgtcaca gctgaaaaca aaaggctcgg cgatgacgga
     2881 aacattaatt ttacttttaa aattttgtcg gcaatcggca ttattatcat agtttcgggg
     2941 cattgctaca acggcggtgt gtcgctgatg tatgattggt ttccgaagta ttcgtacaat
     3001 ctcgccttgt tcgttttcat ttcgggctat ttttacaagg aaaaacacga ggaacatatt
     3061 ttcaaatata tatggggacg gacaaaacgc ttgttgattc cagcgtattt atggaacatt
     3121 gtttacggaa taatcgcatt cgcacttact caggtcggct atacgattgc tcagggcaaa
     3181 tttgatctgt acaacctttt tgttatgccg tttattgacg gcgaaagttt tgcctacaac
     3241 ctcggttcgt ggtttgtata tccgctgttc tgtgtttatg tttttaacat actgttccgc
     3301 aaactgctca aaaaggttca caggggcaac gagtatgtca ttcttgccgt gtatcttgca
     3361 atcggaattt tcggtgtgca atactgcatt tggaatcctg ccccaaaggg tgcgttgctg
     3421 ttattgttca ggtcaatgtt ttttctgccg tgttttgagt ttggcagatt ttatcacgaa
     3481 aagcttgaaa aacacgataa tctcaacagc gttgcatatt ttgcaatcgt tctcggagtt
     3541 cagcttcttc tcctcacatt ctgtgaaaat cttgaataca ctccgtcaaa gtgcgtgaag
     3601 tttgacaacg gctgcgtcat tccttatgta accgcaatca cgggcattgc gttctggctg
     3661 agggttgcaa agctgattac tccgattatc aagaacaaaa agcttgtcag gatgatttcg
     3721 gacaatacct acagcattat gattcaccac atttttgcct ttatgattgt aaaatgggcg
     3781 ttctggggat tgagcgtaat tacaccgctg ttcaaagatt tcgatatttt aaaactcaag
     3841 tcaacaatct ggtacattta tcgtccgaac ggcttaaatt tttactgcat aatctacctt
     3901 gccgcaggaa tattcattcc gattcttata ggaacggtta cgcacaggat atttgaattt
     3961 atgaaagagc ataccttgaa gagtatgcaa aaaaagcagt cggacaggta aagtaaacaa
     4021 aattgaaaaa gtcgtaaaat aatgcttttt ttacaggctt taaaatgaat aaaaaataag
     4081 ctgtatgtgc attgttacaa aataacacgg cttgtattgt gcaatatttg cctttaaagc
     4141 tgtgcgactg aacaacttgc actaaaaggt aaaataatat ttattcattt cgccgtagca
     4201 aaaaacatta atttgtgtta caatatttca ttatagtatt tagcggagca gatatgccga
     4261 tatacagaat agctgatttg aatgttgaag ttaatccaaa atttaaaata tgcaggaaac
     4321 ggcttgaaag ctatgccgtt aagtcgaatg aatcgggaaa gaccgacatt gcgatatcgg
     4381 tttcggatga cgaaatccgt gagcgtctca ggctttccga caacctcagc gaacaaggtg
     4441 cggaggatgt tctgatactc tcaaaattct gcaacgccgt gcttgacgga tttgacggat
     4501 tcttttttca ttcgtcctgt cttgagcttg acggcgaggg ctatgttttt tcggcagtca
     4561 gcggaacggg caagtctacc cacacggctc tgtggcgaag gcattttggc aacagggtga
     4621 cgatgattaa cgatgacaag ccggttatac gaaaatgtga cggaaaattt tatgcttacg
     4681 gaactccgtg gatggggaaa gctgacatag gaacaaatac aaaagcaccc gttaaggctg
     4741 tgtatattct tcagagagca gaagagaaca cggcagtcag ggtgttaccg tcacaggtta
     4801 tgaaacagtt gcttgaggca acggttattg atgttgagcg gagcagaatg gaaaagctgc
     4861 ttgaattgct tgatgagttc tttaaggaaa caccgttgtt tttgctcggt tgtaatatgg
     4921 atgaaagtgc tgttatgacg gcttacaatg ccgcaaacgg cggataaggc tatggaaatt
     4981 tcagaaagcg gaggaatata agatggaatt tgttcaggtc gtttcggttg aaaatatgag
     5041 aaaaagcgac gcaaggacga ttgcggagca tactccttcg gcggagctta tgtacagagc
     5101 cgcacagggg atttttaatt cggcaaagtt tgtgggcagg gttgccatag tttgcggtaa
     5161 gggcaacaac ggcggcgacg gttatgcgct cgcctgtgtt ttatgcaaaa acggctttac
     5221 accgacaatt ttcagagcaa gcgacggctt ttcaaaggac ggcctttact attataaaac
     5281 cgcaatgtcg ctcggtgccg aggagatgcc gatgtcacag gcggcggctt tcacgggatt
     5341 tgacattgta gttgactgtc tgctcggaac aggcttttcg ggtgaattaa agggtgaaat
     5401 gcttgaagcg gttgagcaga ttaatatgac aaatgcctat gttataagtg cagacatcaa
     5461 cagcggaatc aacggtgaca cgggtgtgtg cagtacggcg gtaaactcgg atcttaccgt
     5521 ttcaatcggt tcgttcaaga caggcttgtt cctcaatgac gcaccgtatt acatcggttc
     5581 agtcacaaac tgcgatatcg gaatcagcct tattgaagat gaatacaagc tgattgacta
     5641 ttctcttttg catatgtttg agggctacgg ctctcttgtt atgactgcgg aagaattttt
     5701 tgaaaagtac ggctacgagc cttcaaagtg caatgttgca cgctgtgttg aggaaatcag
     5761 caaaaaagaa cgcagaacag ttgtagtaaa aacagaccat tctgcggtaa ttgcggatct
     5821 taaatatatt tatttttgcg ctgactatgt tattaacaat tgattgattt taattgagtt
     5881 gaggatgaaa ccatgaagta caagaaggtg ttgttaccgc ccgagccgta ttacagcggc
     5941 agggttacaa gatatccgga cagaaaagtt gcctttgaat cgggactcag gtggcatggt
     6001 tcatatgaaa tttcctatat aaaaaaaggt aaggtaaggc ttaacaagct tgactgtgaa
     6061 attattctca atgcgggcga tgtctatgtt cttaacagtc aggaggttca ttcctatgag
     6121 gacatcggtg aggatactca ggctgttatg ctcaatatca ttccaaaggc aattgcaccg
     6181 tatgttgacg atccgcatat tgtgccgagt ttcaaacaac ccgagggcga agctcttgag
     6241 tttatcagaa aagcaatgga catattccac ggatatgaag agtacgagag caagcttatc
     6301 agcatgaagg taagagcggt gatgtacagc gttgcgtact atctcatccg tgattgcatt
     6361 gacaacgata tacaatatgt tcacggttct caggctgatg attttgactg cgccaaaagt
     6421 gctatcagat atatgcacga taattacaaa cacgaaatca cgctcaatga aatcgccaat
     6481 tatgtaggca tgacgcctgc tcatttttca aaatatttta aggataaaac agatgaaaca
     6541 ttttcaaagt atcttcgccg tgttcgtctt gaacacgcta ttgatgattt gagaaacaaa
     6601 aaaatcaccg tcagacaggc ggcgattgaa aacggttttc cgaatgtaaa ttcgtttatc
     6661 ctgttctgca agtccgaata cggcagaact cctgcggaac ttaggaatta cagcgagaag
     6721 aactgacggc gagccaccgt tctcggttcg tgaagacagt cgtatcgatg cgaaagcttt
     6781 ttaaattgaa aatggaaaat tgaaaattgc ggtcggcggt gtgccaccgc aatctattcg
     6841 agaagatact cggataaagg aattgcatca gatgcccgaa tacggcattg atgcaatttt
     6901 tgctttatat gaaagtaaat gtatacttgt aggggagaac cgatgttata caaccgtcac
     6961 aacacctatt tcaacttgat tattgattct ggggatgaat acggtgttgc tcagacctgc
     7021 gcagacaatc atttttgtgc cgtcaaggtc aaattcgcct ttgtcatatt tcggaaaaag
     7081 tccctgattc ggagcgatta ctccgccgat gaacggcaat tgaatctgtc ctccgtgggt
     7141 gtgaccgcag acgattaagt ctatgtcggt tttctttgcg tcctcggcaa tgtattcggg
     7201 ctggtggtgc agaagaattg tgaaattgtt ctttgctttt tcggcaaatt cttcccacag
     7261 cgttcttgac ggaacatcgt catcgggagt gtagaactca taataagggt agtcgctcat
     7321 tccgccgata agcacacttt tgccgttttt ggtgaatttt atgtattcat tatcgagcaa
     7381 tacagcgcct gttgaatttg tttcgctttt aaaatccgtt ccgaatttgg cggcattgtc
     7441 acgctcatgg ttgccgagac agcagtatgt gggtgcaatt tttgaaagct tttcaagcac
     7501 atctttcatc ttcgttgtgt cgggatcgcc acggttgacc atatctccgc aaactgcaat
     7561 aaaatcggga ttttgttcct ttacaagctc tgcaaggtct gcgttctttt ctccgtattc
     7621 cttgttgtgc aaatccgtga tgacaacaaa tttcagcttt gcgtcgttct tagtattgtt
     7681 tacacagtaa tttgtgattg caattgtgtt gtcacagtaa acgccccata ttacgagagt
     7741 cacaaacagc acaagtatta ttgctccgag gattatcgaa aaatttttca ttcaatttac
     7801 cgcccatatt aagtatggta ttattttagg ctcgttttgt gcggttgtca acaaatgtgt
     7861 gtcgaacaag tctgattatt actgttcctt gtgctttgat tgtcggattt taagttgatt
     7921 taggaattgc tttgtttgac attccttttg cactgttgta taataaatca taattgtatt
     7981 tatatgttac tcgggaaatt tacacaaagt taaacagaag aggtatgggt tatgaagaag
     8041 attaatttgc gtaagatgtt tctggctctc gcagatgtgt ttattatcgt tgtcagcggt
     8101 attcttgcaa attatattct cttgcttgtc ggctacatag gtgctgcttc aagcaaaggc
     8161 ttgttgagct atattgttat tgatcttgtc atgtgtctgt ttacgcttta tataagcgga
     8221 acatattcaa aattgtggcg atactttaac gcaaaggact atattgtgtg cggaactgcc
     8281 gtcttttcgg ggtttacgct cggctttgta atctcgttgt ttttgcagat tccgcagaga
     8341 aagatttttt gtatagtaca tttcgcaata gcccttgtcg gaattcttgc attccgtttt
     8401 gttttcagaa gggcttttct tgaccttacc gaggcaggaa gggcagaggg cggagagcgc
     8461 acccttattg tgggcgcagg caatgccggc agaatgattg ttcgtgaaat tcacaatgcg
     8521 aaagagcagg gtgatgttaa taatccttca aaaagcatca ttccggtatg ctttgtcgat
     8581 gatgatttga aaaagctgaa tcagaaaatc gaaggaatcc ccgttgtcgg aacctgtccc
     8641 gaaattgtta aaatatgtga tgaataccgc atagacaata ttattgttgc agttccgtca
     8701 tgtgaggaag atgagaagag gaagattctt gactactgtt ccgcaacaga gtgcaaaatc
     8761 aagattatcc cttatctcgg tgagcttttg tttgatgacg ggcatacaca gcttatttca
     8821 caggcaaagg aaatcaagat tgaggatctt ctcggcagaa aacccattga gtttgaccga
     8881 aaggagattc atgaccttat caacggaaag gtttgtcttg ttacaggcgg cggcggttca
     8941 atcggcagtg agcttgtaag acagattgca aagaatgatc cgaaacaaat catcattgtt
     9001 gatatctacg aaaacaacgc atatgacatt cagcaggagc ttgtgcttga atacggcaca
     9061 aagcttaacc ttgtaacgct tatttcatcg gttcgtgatt acgacaaaat ggagcttatc
     9121 ttcaagacat atcgccccga actcgtattc cacgctgccg ctcacaagca tgttccgctt
     9181 atggaaaccg ttcccgagga ggctgtaaag aacaacatct tcggcacatt caatgttgca
     9241 acccttgcag aaaagtacaa ggcaaagaag tttgttatga tttcaaccga caaggctgtt
     9301 aatccgacaa atgttatggg cgcaacaaag cgtgcgtgcg agatgattat tcagtacaag
     9361 gcgcagcaca gcaccgatac cgaatttgta acaacaagat tcggtaatgt tctcggctca
     9421 aacggctcgg ttattccgct gtttcgccgt gagattgaaa acggtgctcc cgttactgta
     9481 acacatcccg atattatcag atattttatg actattcccg aggctgtttc gcttgttctt
     9541 gaggcaggtg caatggcaaa gggcggtgag atttttgttc ttgatatggg tgctcccgtc
     9601 aagattacaa cccttgccga gaaccttatc agaatgtacg gtaaggttcc gtacaaagat
     9661 gttccgatta tcttcacggg acttcgtccg ggtgaaaagc tttttgagga gcttcttatg
     9721 gacgaagaag gtcttaagtc aacacagaac aagaaaattt tcataggaaa tcaaattaaa
     9781 atagacgaaa cagatatgct gtcaaagctt gaggttctca aaaagaccgc agaaaacaat
     9841 gacagtgaaa agacagttga acttttggca gaacttgtac ctactttttg ccacaaggtc
     9901 aatgattaac cgaaaaggag aaattttatg tgcggtattg taggttatgt aggacctaag
     9961 gattgcagtg aggtgcttgt taatgctctc acaaagcttg aatacagagg ctatgactcg
    10021 gcaggtattg cggtttttga taacggagaa atcaagaccg taaagaccaa gggtaagctt
    10081 caggacttaa aggataagct tgcagaggtc ggcactccga aaggtcatgt cggcataggt
    10141 cacacccgtt gggcaaccca cggcgagcct tcggatgtaa actcacatcc acacagcggt
    10201 acaagggtta caatcgttca caacggtatt attgaaaact atatggagct taaagacttc
    10261 ctcatctcaa agggaagaac ttttttaagc gacacagata cagaggttgt tgctcagctc
    10321 ctcgactata aatacaacgg aaatcctctt gaaacaattg acagtgtaat ggctgagctt
    10381 aagggttcgt ttgcactcgg aattatgttc aaggatttcc ccgacagagt ttttgcggtt
    10441 cgccgtgaaa gtcctcttat cgtaggcgtt gcagagggtg agtgctttat tgcgtccgat
    10501 gttcccgcaa ttcttcagta cacaagggat tattatcttc ttgcccatga tgaaatcgta
    10561 acactttcac ctgacggtgt ttcgttcgtt gacgaacacc ttgatccgat tgaaaaggaa
    10621 cttcagacag ccgactggga tatggaggct gccgaaaagg gcggttatcc gcactttatg
    10681 attaaggaaa tcaacgaaca gcccgaggct atcagaacaa cgattatgcc gagaatcaaa
    10741 gagggacttc cgtttcttga ggagtgcggc atcacaacgg aaacaatcaa gaatttcaaa
    10801 aatatcacaa tcgttgcctg cggtactgct atgcacgcag gtatggtcgg caagtatgtt
    10861 attgaggata tcgcaagagt tcccgtaaat gttgacattg cgtccgagtt ccgttacaga
    10921 aatccgattg taggcgaggg cgaccttgta atcatcattt cacagtcggg cgaaacagcc
    10981 gacagccttg ccgcaatgcg tcttgcaaag cagaagggtg caaaaactct tgcaatcgta
    11041 aacgcaaagg gcagttcaat tgcccgtgag gcggatatgc tcatctacac actcgcaggt
    11101 cccgaaattt ccgttccgtc aacaaaggca tacatcacac agctttccgt tatgtatctc
    11161 tttgcctttg agcttgctct tgcaaaggaa acaatcagca cagccgagtg caagcgactt
    11221 gtttcggctc ttacaaaaat gcctgaggca gttcagtatg taatcgacaa ctgcgaggat
    11281 acctgcaagt tcgttgccac aaagcttgtt gcaaccgaca gcttgcttta cataggcaga
    11341 ggtcttgact atgccctttc aatggagggt tcgctcaagc tcaaagaggt ttcgtacatt
    11401 cactcagaaa gctacgccgc aggcgagtta aagcacggca caatttcgct tattgacgag
    11461 gatgttcccg ttatcgccgt tgcaactcag acaggtatta tccccaagac aatcagcaat
    11521 gttgttgagg ttaagtcaag aggtgcaaag gttattcttg tttgtacaga cgcctgtgca
    11581 agagaactca aggacggtgt tgcggattat gttatcgaaa ttccgcatac agacgagctt
    11641 cttatgccga ttacagcggt tgttccgctc cagattcttg cttattacac atcaatcaac
    11701 agaggtattg atcccgataa gccaaagaac cttgctaagt cggttactgt agaataaacg
    11761 gcggtcttgt atttaattga aaattgaaaa ttgaaaatgt aaaatttcgg ttggcggtgt
    11821 gccaccgcag tcaattcgag aagatagtca gataaagaaa ttgcattgat gcccgatacg
    11881 gcattgatgc aatttttgtt ttatatgaaa gtgaatgttt atttatataa ttttaatttg
    11941 tacaaatctt tgcgtggtta tctgcaggcg gataatatcc gtacatacag ttttgcaaca
    12001 acttccgtat ataacaaaaa tatacaaaaa cagtcgggcg acaatgtttt gcattcttgc
    12061 gaattgtctt ctcgaccata attttccatt ttccattttc cattttcaat tttcaatttt
    12121 atgcacgctc aggcgtaata tgttctttaa tatcatactg tattcacgaa tagggtgctc
    12181 ttgttttcaa atttaaacgg taaaaacctt gtattttttt gacagattat gtataattaa
    12241 ttagatatac attctaaaaa gcagtattga tgaatctaat ttagtttcgg gggaatatac
    12301 ggacatgatg aaaactgttt ttaaaagagc tgtaacgctt gcaatttgtg ccgtaatggt
    12361 attttgcggt atttttgcct gttcaggcgc aacaaaagat tacgagattc cgcagattga
    12421 cgatatgacg ctttcaatgc ctgacggtat gagcgctgtt acaagggaca gtaaaagcac
    12481 agataaatat ttttctgttt tcggtcttga ttatgacaca acaatgaaaa cctttcagaa
    12541 cggaaatatc tatcttcagg gtatggataa tatgtcatcg gttattctga ccgtgacaat
    12601 gacaaaaaat gagagcagcg agaatgtcgg caactatgct cagctttctt caagcgagct
    12661 ttcaaatata cgcaacaatt ttcttaattc cgacgagtat cggagctgta ctcccgacca
    12721 aaaagaaaaa gttgtgtggc ttgtgtttga cgcaactgct cagagcagta agggcgatgt
    12781 gaaaaccttt gttgccaaca cggtttatga cggtatgaac attaatatca caatgcagag
    12841 aacagagggt gatgttacaa ccgttgacta cgatactttt tccaaaattg tatcttcggt
    12901 aaaattcagc gacctgccgt tttcaaaggg atttattccg tacataatca tcggcggcgg
    12961 tgttgttatc cttattttga ttgttctcat cataattttt gccgtgagaa tcagaaagca
    13021 taaaaaggct aagaagaata acgctattct tgaggagctt gcagtcaagt ataaactcgg
    13081 tgaaaagggt acggactacg acattgacaa ttttgacgat tctcccgaaa agcccgataa
    13141 acgctcaaag aaaaagcaga aatatgcgga agaggttgaa gaagaaccgg ctttgccggg
    13201 cagaagatat gctcccgagc cgataatcga aaatatggaa gaaccggaag aaattacaag
    13261 tgagtattca aactttggtt atgacacgga tgatgccaag gaaaaagaaa ttgacgagat
    13321 aatcaactcc gcaaaggctt acaagagtga gcttgagcgt gaggcatata aaaacaacta
    13381 cggttacgag gaaaacagca cggaggacga aaaaaaaaag gagcctaccg cagcagatac
    13441 ctcagagact gaacccgaaa aaattgacga aaacgaaaaa gataacgcag aaacacctga
    13501 aaaacacgag gacataagct cttcgtcagg tcctacactc gttatccccg aacccgaaca
    13561 cgatcttttt gaaacaagcg agcctgacga tatcgaccgt gaggacgatg ccgatgttga
    13621 agaaaccgag gaggtttccg aacaaaagca tccgcttgaa aaaaaggttg acaagtacgc
    13681 aaacatcatt ttcggcagtg acgatcccga ggagaaaacc ggggaagagg ttgatgatga
    13741 ggagcttgta agggcaaagg ctaagaaaaa caagtttgac agcggttatg acttttttga
    13801 cgaagctccc aaaaagtcaa tgggggttat caaaagctca gacctcagag atgccgagga
    13861 ctttgatgtt atcggcgagg ttgaaagaaa agcagaaacc gtaaagcaga acgctcttga
    13921 aaaagagaat aataaagaaa ccgagggtga accgaccgat aaacggtcaa cggaagaggt
    13981 tcttgccaat gtaggcaaaa acatcggtaa cgctctcagg ggctttgcag gcggagttaa
    14041 gaattttggc attcactgcg gatatttctg caagaatgtt tcaataatga taaagcataa
    14101 gcgtgcgatt gccaaacgca aaaaggcaga ggaagagaga agagagagag aaagacaaaa
    14161 ggcagagcgt gcaagaatgc gtgccgaaag aggcgaaagt gacggacttg ttcaggttca
    14221 cagcagaacc gaccgccgcc cacagcagaa tcgccgccct gcaaacagaa acaagagaag
    14281 ataatttctt ttcttaacgg aggaaaaaca tggaaatgct aattaaaaac gcagtggttg
    14341 tttcccctgc cgacaagctt aacgaaaaaa cagatattct cataaaggac ggtgtgattg
    14401 ccgaaatcgg cagcggcatt acatcagacg gagagattat tgatgcagac ggtctttgtg
    14461 ccgttccggg atttgttgat atgcatgtgc atcttcgtga tcccggtcag actcacaaag
    14521 aggatattat cacgggatgc aaggcggctg ccgcaggcgg tgttacaagc ctgcttgcaa
    14581 tgcctaacac caaccccaca accgacagcc ccgaaattgt aaagtatatt cttgacaaag
    14641 ctaaagacgc cgacagccgt gtttatgttg cggcaagcgt taccaagggg cttaaaagcg
    14701 aagaacccac agacattgac gctgtgaaaa gtgcaggtgc aatagcactt accgatgacg
    14761 gcagacctgt tgaaaacaca aagtatttga gtgacgcaat gaaaaaagct ccaaagctcg
    14821 gcatgacaat tgtcgcacat tgcgaggact tgtttcttgc cgacggcggt aaaattaacg
    14881 agggcgaggt ttcggaaaag ctcggtgtca agggaatccc tgccggtgcc gaggactgcg
    14941 gaactgcaag agaaattgca cttgccgccg ctcttaacga gcctattcat atctgccatg
    15001 tcagcaccaa aacaagcgtt gcacttatcc gtgatgccaa aaggagaggt gttaaggtga
    15061 ccgccgaaac cgctccgcat tatgtttcgc tcacggacag ggagctttta agaggtgacg
    15121 ctgattacag aatgaatccg cctctcagaa ctcaggctga tgttgacgag attatcaacg
    15181 ggctttgtga cggtacgctt gatgccatag caaccgacca tgcaccgcac acacccgagg
    15241 aaaaggctga ttttgtaaaa gcccccaacg gttcaatcgg catggaaacc tcgtttgcgg
    15301 tctgcaacac atacctggtt aagacaggca gaatgacgct ttcacagctt gttgagaaga
    15361 tgagtgttaa tcctgcaagg attttgaaaa tcaacgcagg cacactttca gtcggtgcaa
    15421 atgccgatgt tgttcttata aatcccgatg aagaatggac agttgatgct ggtaaacttc
    15481 acggcaaaag caaaaacacc ccgtttaaag gcagaaccct cacaggtaag gtcaaaaaga
    15541 caatcctcgg cggtaaggta gtttttgaag attgagcaaa taaaacccgt aggggcggat
    15601 attatccgcc cgcaagtaat agtgcgaaat tgttgttaaa acaaattata taaatgaaac
    15661 aaagttaatg atatactgta gcggcgaacc gtgtttgcca atcggttaaa atgacagtca
    15721 atgactgtca gtaa
//
LOCUS       DAOMVE010000026        15682 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_774_length_15682_cov_3.485890, whole genome shotgun sequence.
ACCESSION   DAOMVE010000026 DAOMVE010000000
VERSION     DAOMVE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15682
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_15682_cov_3.485890"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..601)
                     /locus_tag="PLS64_02640"
     CDS             complement(179..601)
                     /locus_tag="PLS64_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42762.1"
                     /translation="MKEKIKVTAVVMFLGVIFSGILVAYKLVNTIIPSFDGGSASVFD
                     YLLIVTVTVSMVLKLAVGSMGLRAVSKKTSPPDMQKMLKTSGKLAVVLVLIGVLNIIF
                     NVVNGQVVFVIEVFDIATDILLGFVYILASASVRAGRE"
     gene            complement(631..2043)
                     /gene="gatB"
                     /locus_tag="PLS64_02645"
     CDS             complement(631..2043)
                     /gene="gatB"
                     /locus_tag="PLS64_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRL42763.1"
                     /translation="MSYELVAGFETHIELATKTKIFCGCTTKFGGAPNSHCCPVCIGL
                     PGTLPKLNREVVRYAIRAGLAVHGEIAEISKMDRKNYCYPDLSKAYQISQLYAPLIIG
                     GYVELSNGRKIRLHHIHIEEDAGKLIHQHGDTYVDYNRGGVPLIEIVSEPDIRSIDEA
                     REYVEKLQQVMRYIGISDCKMQEGSMRCDVNISVRPKGSEKLGTRTEIKNMNSINNIA
                     KAMEYEFERQVDLIENGGSVVQETLRYDDATNTTSSMRSKEDAHDYRYFRDPDLVTIH
                     VPKDVVEEIKAAMPELPADKCRRYIDELAIPEKDAQLLTKYRKISEYFDKACEGVKSP
                     KTVSNFIIGQIFRRTETEADKEIFDVAVTPEQLNELVKLLDSGKIRNNLAKATLEKML
                     DSGKGALEFISESDMGGLDENALNDLCKQAVESNPKAVEDYKNGKEKAIKSLVGNVMK
                     NSRGKADPALAESKIKELIG"
     gene            complement(2040..3494)
                     /gene="gatA"
                     /locus_tag="PLS64_02650"
     CDS             complement(2040..3494)
                     /gene="gatA"
                     /locus_tag="PLS64_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRL42764.1"
                     /translation="MGSIEKIHEMLVSKECSCTELTKSYLDEIEKADGELNAYVNVTG
                     EEALKTAEAVDRKIASGEEIGMLEGVPMTLKDNISTKGIETTCCSKILTGYKPIYNAT
                     VWETLAGENAVMLGKTNMDEFAMGSSCETSYFGGAMNPFDSNYVSGGSSGGVASAVGA
                     NIAAYGLGSDTGGSIRQPASFCGIVGLKPTYGAVSRYGLIAYASSLDQIGPITKSVED
                     AAIVFDAISKRDEKDSTSKGFVGDTYSKLNNDIKGMKIGIAKEYLEGVRDDVKEAVLK
                     AADIYKSMGAEIVYFDLPELKFALPVYYIIACAEASSNLGRYDGIRFGYKTEHYNGTH
                     DMVCRTRSEGFGEEVKRRILLGTYVLSAGYYDAYYKKAQNLRGTIVKAFNNAFEKCDV
                     ILAPTVPMTAFEKGHAVSDPIETYLTDICTVPVNIAGLPGVSVPCGFNAKGMPIGMQL
                     IGKSFGEAEILNAAYKYQQAAAENFKDTKWGVKL"
     gene            complement(3507..3785)
                     /gene="gatC"
                     /locus_tag="PLS64_02655"
     CDS             complement(3507..3785)
                     /gene="gatC"
                     /locus_tag="PLS64_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRL42765.1"
                     /translation="MVTREDVENIALLSKLFVPEEELDGLTKSMQEIIDFADTINNAP
                     TDGEEFDDINNLSNVFREDEVVESYDTKEILSNAPEQAEDHFLVRNRE"
     gene            complement(3875..5623)
                     /gene="aspS"
                     /locus_tag="PLS64_02660"
     CDS             complement(3875..5623)
                     /gene="aspS"
                     /locus_tag="PLS64_02660"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRL42766.1"
                     /translation="MIKANEYRTVTCGELREENIGQQVRVAGWVENIRDHGGVMFLDI
                     RDQYGVLQVVVHNDELLKDINKECTVTLSGTVVKRDEETINKKIATGFVEVHTEDIKV
                     LGKCKNVLPFEVATSTNTNEEVRLKYRFLDLRNPKVHNNIMIRSQIITFLRNKMNEMG
                     FMEIQTPILSTSSPEGARDYLIPSRKHKGRFYALPQAPQIFKQLLMVSGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDFEMAFATQEDVFAVAEEVLYETFKKFSNKEVSKPPFAR
                     IPYAESMLKYGTDKPDLRNPLVIVEISDMFEETDFAPFRKKTVRSINVPNAAGQSKKW
                     FKKMEEFALSIGMKGLGYVKVRDDLTFDGPIDKFLKEGDREELISRNGSKAGDVIYFI
                     ADTAQEAPKLAGQIRTEVAKRLDLIDTSRFEMCFIVDFPMFERDEETNQIIFTHNPFS
                     MPQGGLDSLLNKDPEEVLAYQYDIVCNGVELSSGAVRNHDIEIMKKAFELAGYSEDDL
                     KAKFSALYNAFQYGAPPHAGMAPGVDRMIMLLTEEENIREVIAFPMNSNAQDLLLGSP
                     GEVTEQQLREVHIKLR"
     gene            complement(5759..9178)
                     /locus_tag="PLS64_02665"
     CDS             complement(5759..9178)
                     /locus_tag="PLS64_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL42767.1"
                     /translation="MTKSLIKNTFREIKNTKARFISIMAIIALGVGFFAGIKATVPSM
                     YNLARTYTDEQELMDYRLVSTVGFDEDDIKAVKNTDGVTSVMPSYFCDVQTDADSGGR
                     TMRVIALPEKYGDNSVQNKLVLTEGRLPEKSGEIAADSTSFTAEGYKIGDKIRFAKQT
                     GDTDTSTELKTLEYTVVGLVQSPLYIAYQRGTTTVGNGKISDYFYICPEDFAFERYTE
                     LYVKTDISDKFLAYTDDYDNAIEDKAADFEKVGEDRSNSFTVEVIDKAKSDLADAKQE
                     YSDNKEKTEKKLADAKTAIDDGKKELKTKTDDAQKKLDSAKQQIEDGKTQLENSRNEY
                     NTKIADAQAQIAEKESQLNSAEKQYNSSKAEFDKEISKSQAEIENAEYQLQSAKDTFQ
                     TEQEPQLLAGISQAEEAVSALQYQISQTTDEETLAVLNAQLEQAQQKLDELNATYDSA
                     KAQLEESESRLSESKALFEQKKSEGQAQLDYAYSQISSGRTQLDAAKAELAQQSADGQ
                     AQLDSAESKLNDAQSEYDSGVAKLAEEKKNGEKKLSDAQTEYDDGVKKADEEFKKAED
                     KIKDAEEEINSLTEPKWYVFDRSDNPGYSTFSSNADRLGAVATVFPVFFLLVAVLVCV
                     TTMTRLIEEKRTEIGTLKALGYSNTSIVMKFVIYSLLAAVIGSVIGILIGIFTLPFVI
                     YDAYKIMYYIGDITLIPDYTSIIFGIVAAVVCTVVVSVVVCAKSLHEKPAAAMRPKAP
                     KAGKRILLERIKPLWSHMSFNSKLTARNLFRYKVRLCMTVIGVAGCTALIVAAFGLLN
                     SFEPMTHDQFETIYKYDAVVVPKDSGSADNLSFLTDTLDKNSNVKHSMLVSQQECTVT
                     HGNKIKDSDTNLVVPQNPEKFDEIVSLHTRKGKEELKLSDSGVMLSEKMCSELGIKTG
                     DKITLNVDGKKAEVKVSGIFEQYLYNFVYMTPTAYKSLFGSDCTYNMADVALKDTSDS
                     ACDKFGSQVLSDDKIAAVSYIASSLNEFKNMLNSLDMVVTVMIICAAALAFVVLYNLT
                     NINIAERVREIATFKVLGFYNRETSSFIYKENIILTLLGIFVGLFLGNLLTGFIIQTV
                     EVDNVMFGRDIYFTSYLYAAGLTFLFSILVNAVMSFKIKAVNMVESLKSVE"
     gene            complement(9201..9902)
                     /locus_tag="PLS64_02670"
     CDS             complement(9201..9902)
                     /locus_tag="PLS64_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42768.1"
                     /translation="MSLVEFNNVYKRYKTGEIVINAVDGISFKVEEGEFAIVVGASGA
                     GKTTVLNILGGMDSCSEGEIIVGGKDISKFKDKQLIEYRRHDIGFVFQFYNLVHNLTA
                     KENVELAAQICSNPLDSETALDSVGLLERANNFPAQLSGGEQQRVSIARALAKRPKLL
                     LCDEPTGALDYNTGRQILKLLQETCRKDKMTVVLITHNGAITPMADHIIRMKSGKIVE
                     DIYNENPKNVEEIEW"
     gene            complement(10084..10659)
                     /locus_tag="PLS64_02675"
     CDS             complement(10084..10659)
                     /locus_tag="PLS64_02675"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRL42769.1"
                     /translation="MYNTWEELKSACENCHRCELCETRTNVVVGVGNENAEVMFIGEG
                     PGENEDLQGEPFVGRAGKLLDKMLAAVDLDRNKNIYIANMVKCRPPKNRDPKPEEQEM
                     CIEWLRTQVRMIKPKIIVCLGRIAAARLIKSDIKITKEHGQWIEKNGVLMMAMLHPAA
                     ILRDPRRKPDAFDDFLKLRDKINEVCNHTYE"
     gene            10778..11347
                     /locus_tag="PLS64_02680"
     CDS             10778..11347
                     /locus_tag="PLS64_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HRL42770.1"
                     /translation="MKVMVETSAHHVHVTKETLEALFGKGATLTNKKNLSQPGQFACV
                     EKVTVVGPKSEMKMSILGPERSADQVEISLTDARKLGIAAPVRESGDIAGTPGCKLVG
                     PEGEVEIECGVIAAKRHIHLDPKTAEEFGLKDKQLVSVKVGENTGRATTFGDVVIRVN
                     ANYAPAMHIDTDESNAAGLGGTVDGEIIA"
     gene            complement(11415..11939)
                     /locus_tag="PLS64_02685"
     CDS             complement(11415..11939)
                     /locus_tag="PLS64_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL42771.1"
                     /translation="MNSKIDIKICTIDMLTDLQYLCRTTFYETFSDSTDEEDMRKFLA
                     ETYSAEKLSAELANSESVTFIAYKNGTPLGYLKLNIGNAQTEKCLDNALEIQRIYILK
                     SAKGQGIGSAFMQIAENFASEKKLSTIWLGVWEHNEPAKKFYKSKGYEKFSHHTFVIG
                     DDMQTDFLLKKEIL"
     gene            complement(11944..12726)
                     /locus_tag="PLS64_02690"
     CDS             complement(11944..12726)
                     /locus_tag="PLS64_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRL42772.1"
                     /translation="MADAKLHTIKLPYPDKDDRNVWVYVPSHSDGEKLPVVYMTDGQN
                     LFDDNSTPHGSWEVVKAVENEQKSGVGNAVIVGIDNGNAWRDSELTPKSIGEVQHLEM
                     LCEDFKPEGEIFDNFLMNTVIPYVENNFPVCTDRQNVAVCGSSSGGLMSFFSAFEHPE
                     KFGYAGVFSPAFLCYTREDWAKYLSTKIVENMPYLYIYTGNGDELEEMIFESTEMLYE
                     LLEESFYPYDRMNEVILFENAHNEKSWREIFPDFLHTFLSRL"
     gene            12853..13281
                     /locus_tag="PLS64_02695"
     CDS             12853..13281
                     /locus_tag="PLS64_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HRL42773.1"
                     /translation="MTNDIEMLNCVLQNAEMGCQGITSVRKSLKDSKVDGVLCEHLIK
                     YGKLYHCANKMLQNRGAEPHRVSNMTKAMTRYAAQRDLKRDSSSSHIAEMMIKGNTMG
                     VNKMSRKIREYNGNDPHVSLLAKRMLETEEENIKEMSAFL"
     gene            complement(13395..14999)
                     /gene="pckA"
                     /locus_tag="PLS64_02700"
     CDS             complement(13395..14999)
                     /gene="pckA"
                     /locus_tag="PLS64_02700"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HRL42774.1"
                     /translation="MAKIDLTKYGITGTTEIVHNPSYEELFKEETRPELEGYEVGQET
                     ELGAVNVMTGVYTGRSPKDKFIVMDENSKDTVWWTSDEYKNDNHPASEETWKAVKEIA
                     KKELSNKKLYVVDAFCGANKDTRMAVRFIVEVAWQAHFVTNMFITPSAEELENFEPDF
                     VVYNASKAKVENYKELGLNSETAVVFNITSREQVIINTWYGGEMKKGMFSMMNYYLPL
                     KGIASMHCSANTDKNGENTAIFFGLSGTGKTTLSTDPKRLLIGDDEHGWDDNGVFNFE
                     GGCYAKVINLDKESEPDIYNAIRRDALLENVTVDKEGKIDFNDKTVTENTRVSYPIDH
                     IDNIVRPVSSAPAAKNVIFLSADAFGVLPPVSILTPEQTKYYFLSGFTAKLAGTERGI
                     TEPTPTFSACFGQAFLELHPTKYAEELVKKMEMSGAKAYLVNTGWNGTGKRISIKDTR
                     GIIDAILDGSITKAPTKTIPHFNFEVPTELPGVDSGILDPRDTYANVADWEEKAKDLA
                     SRFVKNFAKYEGNEAGKALVEAGPKA"
     gene            complement(15306..15668)
                     /gene="rplL"
                     /locus_tag="PLS64_02705"
     CDS             complement(15306..15668)
                     /gene="rplL"
                     /locus_tag="PLS64_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRL42775.1"
                     /translation="MTAIIEELKGLTVLELSELVHAVEDEFGVSAAAAVAVAGPAADG
                     GAAAAEQTEFDVVLKAAGGNKIAVIKVVREVTGLGLKEAKALVDGAPKALKEGVSKED
                     AEAIKTKLEEAGAEVELK"
BASE COUNT         4201 a         3592 c         3201 g         4688 t
ORIGIN      
        1 ggctttgacg gcttgccaaa ggttgactaa tgcgagtgtc tcgcacccgg ttcgtatagg
       61 cggatattgt ccgcccatac ggttctaaaa gaaaaattac aaaaacaaaa aacggttgga
      121 ttactttttt cgtaaatcca accgttaatt tttttatttg gtaatctaaa atccgaactt
      181 attctctgcc ggcacgaacg cttgcggagg caagtatata cacaaaaccg aggagaatat
      241 cggttgctat gtcgaacact tcaatcacaa aaacgacctg tccgttgaca acattaaata
      301 tgatgttaag cactccgata agcacaagca ctactgcgag tttgcccgaa gttttcaaca
      361 ttttttgcat atccggcggt gatgtctttt ttgaaacggc tctcagtccc attgaaccta
      421 ctgcgagctt cagcaccatt gaaacggtga cagtcacgat tagcagatag tcgaataccg
      481 atgccgatcc gccgtcaaat gacgggataa tcgtattaac cagcttgtat gcaaccaaaa
      541 ttcccgaaaa tataactccg agaaacatta caactgccgt tactttgatt ttttctttca
      601 taagacacac ccccgtgaag gtttaagtta tcagccgata agttccttaa tttttgactc
      661 tgcaagagca ggatctgcct tacctctgct gttcttcata acatttccga caagcgactt
      721 aatcgccttt tccttgccgt tcttatagtc ctcaacagcc tttggattgc tctcaacagc
      781 ctgcttgcac aaatcgttga gtgcgttttc gtcaagaccg cccatatcgc tttcgctgat
      841 aaattcaaga gcacccttgc cgctgtcaag catcttttca agtgttgcct ttgcaaggtt
      901 gttgcggatt ttaccgctgt caagaagttt tacaagctcg ttaagctgtt ccggagtaac
      961 cgcaacatca aagatttcct tgtcagcctc ggtttctgtt ctgcggaaaa tctgaccgat
     1021 aatgaagttt gaaactgtct ttggtgactt aacgccctcg caagccttgt caaaatactc
     1081 gctgattttg cggtactttg taagaagctg tgcatccttt tcgggaattg caagttcatc
     1141 aatatatctt ctgcacttat cggcaggaag ctcaggcatt gcagccttga tttcttcaac
     1201 aacatctttc ggaacatgga tagtaacaag gtcgggatca cgaaaataac ggtaatcgtg
     1261 agcgtcctct ttacttctca ttgaagatgt tgtgttggtt gcgtcgtcat aacggagagt
     1321 ttcctgaaca acacttccgc cgttttcaat aaggtcaacc tgtctttcaa actcatactc
     1381 catagccttt gctatgttgt tgattgagtt catattttta atttcggttc ttgtaccaag
     1441 cttttcgctg ccctttggac gaaccgaaat gtttacatcg caacgcattg agccttcctg
     1501 catcttacag tcggaaattc cgatgtaacg cataacctgc tgaagctttt caacatactc
     1561 tcttgcctcg tcaattgaac gaatgtcagg ctctgaaacg atttcgataa gaggaacacc
     1621 gccacggtta tagtcaacat atgtgtcgcc gtgctgatga atgagctttc ctgcatcctc
     1681 ttcgatgtga atgtggtgca aacggatttt tctgccgttt gaaagctcaa cataaccgcc
     1741 tataataagc ggagcataaa gctggctgat ctgataagcc tttgaaaggt cgggatagca
     1801 gtagttcttt ctgtccattt ttgaaatctc tgcgatttca ccgtgaactg caagacctgc
     1861 acggattgca tagcgaacta cctcacggtt aagctttgga agtgttcccg gcagaccgat
     1921 acacacggga cagcaatggc tgtttggcgc accgccgaat tttgtagtac agccacagaa
     1981 gatttttgtc tttgtggcaa gctcaatatg ggtttcaaaa cccgcaacta attcgtaact
     2041 cacagcttaa caccccactt tgtatcctta aagttttcag cagcagcctg ctgatatttg
     2101 tatgccgcat taagaatttc agcctcgccg aagctcttgc cgataagctg cataccaatc
     2161 ggcataccct ttgcgttgaa tccgcacgga accgaaacac ccggcagacc tgcaatattg
     2221 acaggtacag tgcagatatc cgtaagatag gtttcaatcg gatcgcttac agcgtgtccc
     2281 ttttcaaatg ctgtcatagg aactgtcgga gcaaggatta catcgcactt ttcaaatgcg
     2341 ttgttgaatg ccttgacgat agtgccgcga agattctgag cctttttgta ataagcgtca
     2401 tagtaacctg cggaaagaac ataagtaccg aggaggattc tgcgtttaac ctcttcgccg
     2461 aatccctccg aacgggtacg gcaaaccata tcgtgagtgc cgttgtaatg ctcggttttg
     2521 taaccgaagc ggataccgtc atatctgccg aggtttgaag atgcctcggc acaggcgatg
     2581 atatagtaaa caggaagggc aaatttgagt tccggcaggt caaagtaaac aatctctgcg
     2641 cccattgact tgtagatatc ggcagccttt aagacagcct cttttacatc gtcacggaca
     2701 ccctcaagat attcctttgc aataccgatt ttcatgccct tgatgtcatt gttgagtttt
     2761 gaataagtgt caccgacaaa gccctttgag gttgaatctt tttcatctct ctttgaaatt
     2821 gcatcaaaaa caattgcagc gtcctcaaca gactttgtaa tcggtccaat ctggtcaagt
     2881 gaacttgcgt atgcgataag tccgtaacgg gaaaccgcac cgtaagtagg tttaagacct
     2941 acaataccgc agaatgatgc cggctgacgg attgatccgc ctgtatcaga gccaagaccg
     3001 taagcggcaa tgtttgcgcc taccgcactt gcaacaccgc ctgatgaacc gcctgaaaca
     3061 tagtttgaat caaacggatt cattgcaccg ccaaagtatg atgtttcgca tgaagagccc
     3121 attgcgaact cgtccatatt cgtcttgccg agcataactg cgttttcgcc tgcaagagtt
     3181 tcccaaacgg ttgcgttata aatcggctta tagcctgtga gaatttttga acaacaggtt
     3241 gtttcaattc cctttgttga aatattgtcc ttgagcgtca tcggaacacc ctcgagcata
     3301 ccgatttcct cgccggaggc aatttttctg tcaacagcct cggcagtttt cagagcctcc
     3361 tcgcctgtta cattaacata tgcgttaagc tcgccgtcag ccttttcgat ttcgtcaaga
     3421 tagctttttg taagctcggt acatgaacac tcttttgaca caagcatttc atgaattttt
     3481 tcaatagaac ccatttttac accgccttac tctctgtttc ttacaaggaa gtgatcctct
     3541 gcctgctcgg gagcatttga aagaatttcc tttgtatcgt aggactcaac aacctcatcc
     3601 tcacgaaaaa catttgacag attgttaata tcgtcaaact cctcgccgtc tgtcggagcg
     3661 ttattgattg tgtcggcaaa atctatgatt tcctgcattg actttgtaag gccgtcaagc
     3721 tcctcttcgg gaacaaaaag ctttgaaagc aacgcaatgt tttcaacatc ttctcttgtt
     3781 accataaaaa ccgtcctttt taaagaaaat agggcaagtc atataatata taactcgccc
     3841 tttgagtttg aatatatgac tgcaaatgca gtttttatct taatttaata tgaacctcac
     3901 ggagctgctg ttctgttacc tcacccggtg aaccgaggag aagatcctga gcattggagt
     3961 tcatcgggaa tgcaataact tctctgatgt tctcttcctc tgtaaggagc ataatcatac
     4021 ggtcaacacc cggagccata cctgcgtgcg gcggagcacc gtactggaac gcattgtaga
     4081 gtgcagagaa cttagccttg aggtcatcct cgctgtagcc ggcaagctcg aacgctttct
     4141 tcataatttc aatatcgtgg ttacgaacag cgcctgatga aagctcaaca ccgttgcaaa
     4201 cgatatcgta ctgataagca agaacctctt cgggatcctt gttaagaagg ctgtcaagac
     4261 cgccctgcgg cattgagaaa ggattgtgtg tgaagataat ttgatttgtt tcttcatctc
     4321 tctcaaacat cgggaagtca acgataaagc acatttcaaa acggcttgta tcgataaggt
     4381 caagacgctt tgcaacctct gttctgatct gacccgcaag ctttggagct tcctgagcag
     4441 tatcggcgat aaagtagatt acatcgcctg cttttgaacc gttacggctg ataagctctt
     4501 cacggtcgcc ctctttaagg aacttgtcaa tcggaccgtc aaatgtgagg tcatctctaa
     4561 ccttaacata gccaaggccc ttcatgccga ttgaaagagc gaactcctcc atcttcttga
     4621 accatttctt tgactgacct gcggcattcg gaacattgat tgaacgaact gtctttttgc
     4681 ggaacggagc aaagtctgtt tcctcaaaca tatcgctgat ttcaacaatt acgagcggat
     4741 tgcggaggtc aggcttgtct gtaccgtact tgagcatact ctcggcatac ggaatacggg
     4801 caaacggagg ctttgaaacc tctttatttg aaaacttctt gaatgtttcg taaagaacct
     4861 cttcagcaac agcaaataca tcttcctgtg ttgcaaaagc catttcaaaa tcaagctgat
     4921 agaactcacc cggtgaacgg tctgcacggg cgtcctcatc acggaagcaa ggtgcaatct
     4981 ggaaatacct gtcaaagcct gacaccataa ggagctgttt aaaaatctga ggagcctgtg
     5041 ggagagcata gaatctgccc ttgtgctttc tgctcgggat aaggtagtca cgagcgccct
     5101 ccggagatga agttgaaagg ataggagtct gaatctccat aaagcccatt tcgttcatct
     5161 tgttgcgcag aaatgtgata atctgcgaac gaatcataat attattgtga accttcgggt
     5221 tacggaggtc aagaaaacga tatttaagtc ttacttcttc attggtattt gtagaggttg
     5281 caacctcaaa cggaagaaca ttcttacact tgccgagaac cttgatgtcc tccgtgtgaa
     5341 cttcaacgaa acctgttgca atcttcttgt tgattgtttc ttcgtcacgc ttaacaacag
     5401 taccgctgag agttactgta cattccttgt tgatatcttt aaggagttca tcgttgtgaa
     5461 caacaacctg aagcacaccg tactgatcac ggatatcgag gaacattaca ccgccgtggt
     5521 cacggatatt ttcaacccag cccgcaactc tgacctgctg tccgatattt tcttctctta
     5581 actcaccgca ggtgactgtg cggtattcat tagcttttat cattactgta actatccttt
     5641 cacagatatt ttgcctgtca gcctatttat ttagcctatt cttctgtatt ataacacaat
     5701 tgctgtattc aatcaatatt ttacaaaaga ttttttaagc atacggcaaa atattccgtt
     5761 attcaacgct tttaagactt tcaaccatat taacggcttt aatcttaaag ctcataaccg
     5821 catttacaag aattgaaaac aggaatgtca gccctgccgc atagaggtat gaagtgaaat
     5881 aaatatctct tccgaacata acgttgtcaa cctcaaccgt ctgaataata aagcccgtca
     5941 aaaggtttcc gaggaacaga cctacgaaaa ttccgaggag agtcaaaatt atgttctctt
     6001 tgtaaatgaa tgatgaagtt tcacggttgt agaatccgag aaccttaaag gttgcaattt
     6061 cacggacacg ctcggcaatg ttgatattag tcaggttgta gagtacaaca aaggcaagtg
     6121 ccgctgcgca gattatcata accgtaacaa ccatatcaag cgagttgagc atatttttaa
     6181 attcgttcaa gcttgatgca atgtagctta ccgccgcaat tttatcatct gataaaacct
     6241 gtgaaccgaa tttgtcgcag gcggaatccg aggtatcttt gagtgcgaca tcagccatat
     6301 tataggtgca gtcactgccg aacaagctct tataggcggt tggcgtcata tagacaaaat
     6361 tgtaaaggta ctgttcaaaa attccgctga ccttgacctc tgcctttttg ccgtcaacat
     6421 tcagtgtgat tttgtcgccc gtttttattc caagctcact gcacattttt tcactgagca
     6481 ttactccgct gtccgaaagc ttgagttctt ctttgccttt tcttgtatgc agagaaacaa
     6541 tttcgtcaaa cttttcggga ttctgcggta caacaagatt tgtgtcgctg tccttaatct
     6601 tattaccgtg tgttaccgtg cattcctgct gtgaaacaag cattgagtgt ttgacattgc
     6661 tgtttttatc aagtgtatct gtcaaaaacg aaagattgtc ggcacttccg ctgtctttcg
     6721 gaacaacaac ggcgtcgtat ttatagattg tttcaaactg atcgtgtgtc atcggttcaa
     6781 agctgttgag caaaccgaat gctgcaacaa tcagagccgt acagcctgcc acaccgataa
     6841 cagtcataca aagtcttacc ttgtatctga acagatttct tgcggtcagc tttgagttaa
     6901 agctcatatg tgaccacaac ggttttatgc gttcaagcaa aattctcttg cccgcctttg
     6961 gtgctttcgg ccgcatagca gccgcaggct tttcgtgcag agattttgca cacactacaa
     7021 ccgacacaac aaccgtgcag acaactgcgg caacaattcc gaaaattatg cttgtatagt
     7081 ctggtatcag cgttatatcg ccgatgtagt acataatttt gtaagcatca taaattacaa
     7141 acggcaaggt gaaaatgccg attaaaatac cgattacact tccgataaca gccgcaagca
     7201 acgagtagat tacaaacttc attacaatgc ttgtgttgct gtagccgagt gctttcagcg
     7261 ttccgatttc ggtacgcttt tcctcaatta gtcttgtcat tgtggttacg cagacaagca
     7321 ccgctacgag caggaagaat acgggaaata ccgttgcaac agcgccgagt ctgtctgcat
     7381 ttgaagaaaa tgttgagtaa cccgggttgt cgcttctgtc gaagacatac cactttggtt
     7441 ctgtaagaga attaatttct tcctctgcat ctttgatttt atcctctgcc tttttgaatt
     7501 cttcatcggc ttttttaaca ccgtcatcgt attctgtctg tgcatcggag agttttttct
     7561 cgccgttttt cttttcctcg gcaagctttg caacaccgct gtcatactcg ctctgagcgt
     7621 cattcagctt gctttctgcc gaatcaagct gtgcctgacc gtctgccgac tgttgtgcaa
     7681 gttctgcttt tgcggcatca agctgtgtcc ttccgcttga aatctgtgaa tatgcgtagt
     7741 caagctgagc ctgtccctca ctttttttct gttcaaaaag tgccttgctt tcggaaagtc
     7801 ggctttcgct ttcttcaagc tgtgcctttg cggaatcgta tgttgcgttg agttcgtcaa
     7861 gcttctgctg agcctgttca agctgtgcgt tcaaaacagc aagcgtttct tcatcggttg
     7921 tctgactgat ttgatactgc aaagccgaaa cagcttcctc agcctgactt attcctgcca
     7981 aaagctgtgg ttcctgttcg gtttgaaatg tatcctttgc cgactgcaac tgatattccg
     8041 cattttctat ttcagcctgt gattttgaaa tttctttatc aaattctgct tttgaagaat
     8101 tatattgttt ttcggcagaa ttaagctgac tttccttttc ggcaatctga gcctgtgcat
     8161 cggcaatttt tgtgttatat tcattgcgtg aattttcaag ctgtgtcttg ccgtcctcaa
     8221 tctgctgttt tgccgaatca agttttttct gtgcatcatc ggtcttggtt ttcagctctt
     8281 ttttgccgtc atcaatcgcc gtctttgcat cggcaagctt cttttcggtt ttttctttat
     8341 tgtccgaata ctcctgtttt gcatctgcaa ggtcgctctt agccttgtcg ataacctcaa
     8401 ccgtaaagct gttgctcctg tcctctccca ccttttcaaa atcggcggct ttatcctcta
     8461 tggcattatc ataatcgtcc gtgtatgcca agaatttatc cgaaatatcg gtttttacat
     8521 aaagctccgt gtagcgttca aaggcgaaat cttcggggca aatgtagaaa taatccgaga
     8581 tttttccgtt gccgaccgtc gtagttcctc gctgatatgc tatgtaaaga ggtgactgca
     8641 caagaccgac caccgtgtat tcaagggttt taagttcggt tgaagtatct gtatcgcccg
     8701 tctgttttgc aaatctgatt ttgtcaccga ttttataacc ctctgccgtg aacgaggttg
     8761 aatcggctgc aatttcaccc gacttttcgg gaagtctgcc ttccgtcaaa acaagcttgt
     8821 tctgaacgga attatcaccg tatttttcgg gaagcgcaat gactctcata gtcctgccgc
     8881 cgctgtcggc atcggtctga acatcgcaga aatatgacgg cataacagag gttactccgt
     8941 ctgtattttt taccgccttt atgtcgtcct catcaaatcc gaccgttgac acaagtcggt
     9001 aatccataag ctcctgctcg tccgtgtagg ttcttgcaag attgtacatt gacggaaccg
     9061 ttgcctttat tcctgcaaaa aatcctactc cgagggcgat aatcgccata attgagatga
     9121 atcttgcttt tgtattttta atttctctga atgtgttttt tatcaaggat ttagtcattg
     9181 tgacacctcc ttttcatttt ttaccactca atttcttcaa catttttcgg attttcattg
     9241 taaatatctt caacaatttt tccgcttttc attcttataa tgtgatccgc catcggcgtt
     9301 attgctccgt tgtgagtgat gagaacaacg gtcattttgt ctttacggca ggtttcctgc
     9361 aacaatttca gaatctgtct gcccgtgttg tagtcaagcg cacctgtcgg ttcgtcacaa
     9421 agcaaaagct tcggtctttt ggcaagtgct cttgcgattg aaactctctg ctgttcaccg
     9481 cccgaaagct gtgcggggaa gttgtttgcc ctttcaagca agcccacgct gtcaagagca
     9541 gtttcactgt caagaggatt gctgcaaatc tgagcggcaa gctcaacatt ttcctttgcc
     9601 gttaggttat gaacaaggtt gtagaactgg aacacaaagc ctatgtcatg ccgtctgtac
     9661 tcaataagct gtttgtcctt aaatttgctt atatcctttc cgccgacaat gatttcgccc
     9721 tcggaacagc tgtccattcc gccgagaata ttcagaacgg ttgttttgcc tgcaccggac
     9781 gcaccgacta caattgcaaa ctcgccctcc tcaaccttga acgaaatgcc gtcaaccgca
     9841 ttgatgacaa tctcacccgt tttgtatctt ttgtaaacat tattaaattc tacaaggctc
     9901 ataaaatcac ccttttcata tgtttcttac agaaaaaatc actgtgcttt gcatactaat
     9961 tataccaaat taataccgca atatcaacag aaatcttacc gtattcgtca tctgtatgta
    10021 caatattttg aaattataca tataaaaaca gcagaggtca cactaaacct ccgctgcaaa
    10081 aaattattca tatgtatgat tacagacctc gttgatttta tcacggagct ttaagaaatc
    10141 gtcaaaagcg tcgggctttc tgcgtggatc acgcaaaatt gccgcaggat gaagcattgc
    10201 catcataagc acaccgtttt tctcaatcca ctgaccgtgt tcctttgtga ttttgatatc
    10261 cgatttaata agccttgccg ccgcaattct gccgagacag acaataattt tcggcttgat
    10321 cattctcacc tgtgttctga gccactcaat acacatttcc tgttcttcgg gctttggatc
    10381 acggttcttc ggcggacggc actttaccat attggcaatg taaatattct tgtttctgtc
    10441 aaggtcaaca gccgcaagca ttttgtcaag cagtttgcct gctctgccga caaacggttc
    10501 gccttgaaga tcctcgtttt cgccaggacc ttcaccgatg aacataacct ctgcattttc
    10561 gttgccgacg cccacaacaa catttgtgcg agtttcgcag agttcacacc tgtgacagtt
    10621 ttcacaagcc gattttaatt cttcccaggt attatacata attttctctc ccaaaataaa
    10681 gatttaaatt tatcaaaaat gttgaaattc caagaataaa gtagtaaaat aaacacgaag
    10741 ccgtacggcg aattctaatt gaatggtggt acacaaaatg aaagtaatgg ttgaaacatc
    10801 agcgcatcat gttcatgtta ccaaagaaac tcttgaggct ctctttggca agggcgcaac
    10861 tcttacaaac aaaaagaatc tttcacagcc cggacagttt gcctgtgttg aaaaggtaac
    10921 tgttgtaggt ccgaaaagtg aaatgaaaat gtcaattctc ggtcccgaaa gaagtgcaga
    10981 tcaggttgaa atctctctta cagacgctcg taagctcggc attgccgctc ctgtcagaga
    11041 atcaggcgat attgcaggca cacccggttg caagcttgtc ggtcccgagg gcgaagttga
    11101 aatcgaatgc ggtgtaattg ccgctaaaag acacattcac cttgatccga aaactgccga
    11161 ggagtttggt cttaaagaca aacagctcgt ttcggttaag gttggcgaaa atacaggcag
    11221 agcaacaaca ttcggtgatg ttgtaatcag agtaaacgca aactacgctc ctgcaatgca
    11281 cattgataca gacgaatcaa atgccgcagg tcttggcggt acggttgacg gcgaaatcat
    11341 cgcataattt cgatttaaac caaatcacat ttttgggaca gtctttaaag gctgtccttt
    11401 tgttttgcca aaaatcataa aatttccttt ttcaaaagga aatcggtttg catatcgtca
    11461 ccaatgacaa atgtgtgatg tgaaaatttt tcatatcctt ttgatttgta gaatttcttt
    11521 gcaggctcgt tatgttccca tacaccgagc catattgttg aaagtttttt ctcacttgca
    11581 aaattctccg caatctgcat aaaggctgag cctatgccct gtcctttggc acttttcaga
    11641 atatagatgc gttgaatttc aagcgcattg tcaaggcatt tttcagtctg cgcattgccg
    11701 atattcagtt ttagatatcc gagaggagta ccgtttttgt atgctataaa ggttacactt
    11761 tcggagtttg caagttctgc tgacagcttt tccgctgaat aggtttctgc aagaaatttt
    11821 cgcatatcct cctcatcggt tgaatcggag aaagtttcgt aaaaagttgt tctgcaaaga
    11881 tattgcaggt ccgtcagcat atcaatggtg catattttta tatcaatttt gctgttcata
    11941 ttatcaaagt ctgctcaaaa atgtgtggag aaaatcgggg aaaatttctc gccaagattt
    12001 ttcattgtgt gcgttttcaa aaagaatgac ctcgttcatt ctgtcatacg ggtagaagct
    12061 ttcttccaaa agttcgtaga gcatttcggt gctttcaaaa atcatctctt caagctcgtc
    12121 accgttaccc gtatagatgt agaggtacgg catattttca acaatttttg tcgaaagata
    12181 tttcgcccaa tcctctcttg tgtaacagag aaatgcagga gaaaacacac ctgcatagcc
    12241 gaatttttcc gggtgttcaa aagccgaaaa gaatgacata agtccgcccg atgaacttcc
    12301 gcagacggca acattctgtc tgtcggtgca tacggggaaa ttattttcaa catacggaat
    12361 gacggtattc ataagaaaat tgtcaaagat ttcgccctcg ggtttgaaat cctcgcagag
    12421 catttcaagg tgctgaacct caccgattga tttcggtgta agctcgctgt cacgccaagc
    12481 attaccgttg tcaataccta cgattacagc gttgcctaca ccgcttttct gctcattttc
    12541 aacagctttt acaacctccc acgaaccgtg cggagttgaa ttatcgtcaa aaagattctg
    12601 tccgtctgtc atataaacaa cgggaagttt ttcgccgtcg gaatgtgacg gaacataaac
    12661 ccaaacattt ctgtcgtcct tgtcgggata cggaagtttt attgtatgta actttgcgtc
    12721 agccataaaa tcacctgttt tttattatct aatagatatt aaaccataaa aacggctgtg
    12781 ttgcaaccta attttttacg gttgcataaa tacaaaaatt cccgtaaata atacctacag
    12841 aggtgatttg ttatgactaa cgatattgaa atgctcaact gcgttttgca gaatgccgaa
    12901 atgggctgtc agggaattac gagcgtgagg aaaagtttga aggacagcaa ggttgacggt
    12961 gttttgtgcg aacatcttat taaatacgga aagctgtatc attgtgcaaa taaaatgttg
    13021 caaaatcgtg gtgcagagcc acacagagtg agtaatatga caaaagcaat gacacgctat
    13081 gccgcacaaa gggatttgaa gcgtgactcg tcatcttcgc acattgccga aatgatgata
    13141 aaaggcaaca caatgggagt gaataaaatg tcacgcaaaa tccgagaata taacggcaat
    13201 gacccccatg tcagcctcct cgccaaaaga atgttagaaa ccgaagaaga aaacataaaa
    13261 gaaatgtccg catttttgta agtataatct caataaaaaa cacccgaaag gaatttttca
    13321 aaaacctttc gggtgaaatt tttatttaga taaaggttgc accaattatg atgcgttaag
    13381 cgcagtcggg caaattaagc cttaggacct gcttcaacga gagccttacc ggcttcgttg
    13441 ccttcgtact tagcaaagtt cttaacaaaa cggcttgcaa gatccttagc cttctcttcc
    13501 caatcagcaa catttgcata tgtgtcacga ggatcgagga tgcctgaatc aacgccgggg
    13561 agctctgtag gaacttcaaa gttgaagtgt ggaattgtct ttgtaggagc ctttgtgata
    13621 gaaccgtcaa ggatagcgtc gatgatacca cgagtatcct taatagagat acgcttgcct
    13681 gtgccgttcc aacctgtgtt tacgaggtat gccttagcac cgctcatttc catcttctta
    13741 acgagctctt cagcatactt tgtaggatga agctcgagga atgcctgacc gaagcaagct
    13801 gagaatgttg gagttggctc tgtgatgcca cgctctgtac ctgcaagctt agctgtgaag
    13861 cctgagagga agtagtactt tgtctgctca ggtgtgagga ttgaaacagg cggaagtacg
    13921 ccgaaagcat cagctgaaag gaagattaca ttctttgcag caggagctga agaaacaggg
    13981 cgaacaatgt tgtcgatgtg atcaatcgga tatgatacac gagtgttctc tgttacagtc
    14041 ttgtcgttaa agtcaatctt gccttccttg tcaacagtaa cattttcaag aagagcgtct
    14101 cttctgatag cgttgtagat atcaggctct gactctttgt caaggttgat aaccttagca
    14161 tagcagccgc cttcaaagtt gaatacgccg ttgtcgtccc agccgtgctc gtcatcaccg
    14221 atgaggagtc tcttaggatc tgttgaaagt gtagtcttac ctgtacctga aagaccgaag
    14281 aagatagctg tgttctcgcc gttcttatct gtgttggctg agcagtgcat tgatgcgatg
    14341 cccttgagcg ggaggtagta gttcatcatt gagaacatac ccttcttcat ctcaccgccg
    14401 taccatgtgt tgatgataac ctgctcacgg cttgtgatgt tgaatacaac agctgtttct
    14461 gagttaagac cgagctcctt gtagttttca accttagcct ttgatgcgtt gtaaacaacg
    14521 aaatcaggct caaagttttc aagctcttca gctgaaggag tgatgaacat atttgttaca
    14581 aagtgagcct gccaagcaac ctcaacgatg aaacgaactg ccattctggt gtccttgttt
    14641 gcaccacaga atgcatctac aacataaagc ttcttgttag aaagctcttt ctttgcgatt
    14701 tccttaactg ccttccaagt ttcctcagat gcaggatggt tgtcgttctt gtactcgtca
    14761 gaagtccacc aaacagtgtc ctttgagttc tcgtccataa cgatgaactt gtccttaggt
    14821 gaacgacctg tgtaaacgcc ggtcataaca ttaactgcgc caagctcagt ttcctgacca
    14881 acttcataac cctcgagttc gggtcttgtt tcttctttga aaagctcttc ataagaagga
    14941 ttgtgaacga tttcagtggt acctgtaata ccgtactttg ttaaatcaat ctttgccatt
    15001 tcagtatcaa cctttctatg ttttctgaat tcacatagat agtggaattg cgacaaatac
    15061 cccttgtcag cgttaatatc caacacccta tgttcgctaa tagtttatca caaaagaatc
    15121 ggctcgtcaa gcaaaacagt aatcaaaagc agttaaaaca gacattgtga ttatattgtc
    15181 catactcttt tattgtaaga taaaaaattg aatttatgct aaagcaaaaa tgacaaattt
    15241 taacaataaa cgcaaaaaag tccgacagaa atctgtcgga cttaaaatgc aagctataag
    15301 taaaattact taagttctac ttcagcaccg gcctcttcga gcttagtctt gatagcttca
    15361 gcgtcttcct tagaaacgcc ttccttgaga gccttaggag caccgtcaac gagtgccttt
    15421 gcttccttaa gaccaagacc tgttacttca cgaacaacct tgataacagc aatcttgttg
    15481 ccgcctgcag ccttaagaac tacatcaaac tctgtctgct cagcagcagc agcgccaccg
    15541 tcagcagcag gacctgcaac tgcaactgca gcagcagctg atacaccaaa ctcatcctct
    15601 acagcgtgaa cgagctctga aagctcaaga actgtgaggc cttttaattc ttcaataata
    15661 gcagtaattt tctctgatgc ca
//
LOCUS       DAOMVE010000027        15606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_785_length_15606_cov_3.167256, whole genome shotgun sequence.
ACCESSION   DAOMVE010000027 DAOMVE010000000
VERSION     DAOMVE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15606
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_785_length_15606_cov_3.167256"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLS64_02710"
     CDS             complement(<1..319)
                     /locus_tag="PLS64_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL42776.1"
                     /translation="MSITVREYGENDINGMISVWNEVVRDGVAFPQTEELDFESGKKF
                     FAEQTCCPVATDENGEILGMYILHPNNVGRCGHICNASYAVSSSSRGKGVGRKLVSDC
                     LERA"
     regulatory      476..572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            627..1808
                     /gene="metK"
                     /locus_tag="PLS64_02715"
     CDS             627..1808
                     /gene="metK"
                     /locus_tag="PLS64_02715"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRL42777.1"
                     /translation="MAKHLFTSESVTEGHPDKVCDQISDAVLDEILSQDKNAHVACEV
                     TATTGMIHVMGEISTDCYVDIADVARKVVNNIGYNDPKCGFDGNTCAVLTSIDAQSPD
                     IAMGVNESLELKDGESDTDNQIGAGDQGMMFGYACDETPELMPMPISLAHKLAKQLTK
                     VRKDGTLSYLRPDGKTQVTVEYDDDKIVGIDTVVISTQHDEDVTLEQIRKDLIEYVIK
                     PIIPAELMNDNTKIFVNPTGRFVIGGPVGDSGLTGRKIIVDTYGGFSRHGGGAFSGKD
                     PTKVDRSAAYYSRYIAKNIVAAKLAKKCEIQLAYAIGVAKPVSIMVDTFGTSKYSNEE
                     LANAVEKVFDCRPQSIIRQLKLTETKYLPLAAYGHMGREELGVEWEKTNKVDELLKAV
                     G"
     gene            complement(2091..2462)
                     /locus_tag="PLS64_02720"
     CDS             complement(2091..2462)
                     /locus_tag="PLS64_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRL42778.1"
                     /translation="MEKVYTKNAPDAIGPYSQAVKVNGLVFTSGQIAINPATGNVEAA
                     TIEAQTEQVCTNLKNVLEASGSSLEKAVKTVCFLKNMEDFGAFNEVYGKYFTSKPARS
                     CVAVKQLPKDVLVEVEVIAEV"
     gene            complement(2500..3693)
                     /gene="ilvA"
                     /locus_tag="PLS64_02725"
     CDS             complement(2500..3693)
                     /gene="ilvA"
                     /locus_tag="PLS64_02725"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HRL42779.1"
                     /translation="MLTLDKIYHASYVLKDVIRQTNIVQARGICDDCDVYLKPENLQI
                     TGSFKVRGSGYKISQLTEEEKARGVIACSAGNHAQGVALAATKYGIKSLICLPDGAPI
                     SKVEATKNYGAEVCMVPGVYDDAYNEALRLRDEKNYTFIHPFDDENVIAGQGTIGIEI
                     MNEMPDVDAVVCAIGGGGLISGVACAIKSLNPNIKVYGVQAEGAPSMVESIKNGEPVH
                     LDSVSTIADGIQVKEPGEHTFEYVKKYVDDIVTVSDDEISSAILKLIESQKMIAEGAG
                     AAPLAAVMFNKLPVKGKKVVCVVSGGNIDVTILSRVIKRGLLMTGRQQTFCVELQDKP
                     GQLVAVSNIIAEKGANVISIHHERGGEGTDVNGCYLRVVLETRDFAQIKEIRDALVDA
                     GFKLV"
     gene            complement(3939..4361)
                     /locus_tag="PLS64_02730"
     CDS             complement(3939..4361)
                     /locus_tag="PLS64_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRL42780.1"
                     /translation="MSKVAVVFWSGTGNTEQMAEAVAEGAKSAGAEVTLANVNDFDSS
                     SVADFDGIAFGCPAMGAEVLEDSEFEPVFNECESALNGKSIALFGSYGWGDGEWMRNW
                     EDTCKGDGANLICESVICNDAPDDDALEACKKLGASLA"
     gene            complement(4407..4961)
                     /locus_tag="PLS64_02735"
     CDS             complement(4407..4961)
                     /locus_tag="PLS64_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HRL42781.1"
                     /translation="MSEDLIIKHCSPTLAGIKTANLFNCPYSSRLECECDVRKLNKMF
                     CKKGIKVLPLKFSNGKALIYIFRPSKLAVDLSDEYACKLLESQGYHSHDAARCIRELR
                     HRLKDSTEFPHEIGLFLGYPPEDVRGFILHKGNCCKYSGCWKVYGNEEKAAKEFAKFK
                     KCSDIYYSKWLQGTPIQKLTVACN"
     gene            complement(5380..6336)
                     /locus_tag="PLS64_02740"
     CDS             complement(5380..6336)
                     /locus_tag="PLS64_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL42782.1"
                     /translation="MAVLQKIDNPYGMIQTVEHCESFSIYRSQDSTGDCEVTVYPVFS
                     GIELVYYDVHMQSCDINLAKGREMLIITHCQEGRIEFEYKNGEYLYLASGDLSIQKNT
                     ENIRHRYCPLSHYHGVSVAIDMNRVPRCFSCILDDVFVSPEELEMKFCSEKPYSIMRE
                     NISIEHIFSELYSVPENIRKGYHKVKVLELLLFLSGLEYKGESEERRYFSRSQVTAAK
                     EAKKYLLAHLDEHITITELADMLGISSTSLKICFKGVYGDTINGYITNCKMQKAASLL
                     KNTDKSVLEIAGIVGYNNGSKFAGAFRRVMNKSPNEYRKSLV"
     gene            complement(6352..6723)
                     /locus_tag="PLS64_02745"
     CDS             complement(6352..6723)
                     /locus_tag="PLS64_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRL42783.1"
                     /translation="MEYVFYGLLAVAFIAFVVLVIRSYRKKLASGCCGASDESVKKNK
                     VADKDKSHYPYEKTLVVDGMVCKNCSARVENALNSIDGVWAEADVSSGRVTVRMKREI
                     EDKVLKKTVNDIGAYTVMKAE"
     gene            complement(6866..7039)
                     /locus_tag="PLS64_02750"
     CDS             complement(6866..7039)
                     /locus_tag="PLS64_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HRL42784.1"
                     /translation="MLEWLAANIGTIIVALIVIAVIAFVVARMVKDKKEGKSSCGANC
                     AHCACHGDCHNHK"
     gene            complement(7073..>7415)
                     /locus_tag="PLS64_02755"
     CDS             complement(7073..>7415)
                     /locus_tag="PLS64_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRL42785.1"
                     /translation="LYPGGWTEIGTAFTGLSGFSFLLFNLLCAPCFAAMGAIKREMNN
                     IKWFWFAIGYQCVFAYVIAFMVFQFGSIFAGGLNVIGLIFAVAVFAFMIYMLVRPYKE
                     ATTLKVKSAKK"
     assembly_gap    7416..7425
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7426..9640)
                     /locus_tag="PLS64_02760"
     CDS             complement(<7426..9640)
                     /locus_tag="PLS64_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015566309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRL42786.1"
                     /translation="MEIKIALAGNPNCGKTTLFNALTGSNQFVGNWPGVTVEKKEGKL
                     KKHKDVTIMDLPGIYSLSPYTLEEVVARNYLVGERPDAILNLIDGTNLERNLYLTTQL
                     TELGIPVVVAINMIDLVKKNGDSINIDELSRQLGCKVMEISALKGTGIMEAAEAAIKA
                     AGSTKTIPMHSFNGAVEHAIAHIEEAAVHNMPEEQQRWYAIKIFERDDKVLAKLDIPQ
                     NVIDHIEKDIQAAEKELDDDAESIITNERYIYIASIIKSCYKKKNKGKLTTSDKIDKV
                     VTNRWLGLPIFAVIMFLVYYISMQTVGTAATDWANDGLFGDGWHLFGIGSSQAAEAEE
                     TYGDSDAIIEAFNAQYGNDDIAEAVDLESKNYSEDAAKAALAELVNLTPSDASVTYSV
                     QDEETLEITETPDTKKSDLEKAVSNYLNTDYKEGYGAPDAATYGIWVPGIPVLIGNGL
                     DAINCADWLNGLILDGIVAGVGAVLGFVPQMLVLFILLAFLESCGYMARIAFVLDRIF
                     RKFGLSGKSFIPMLVGTGCGVPGIMASRTIENERDRRMTIMTTTFIPCGAKQPFIAMI
                     AGAIFGGSPWIATSAYFIGMAAIVVSGIMLKKTKMFSGDPAPFVMELPAYHLPTVGNL
                     LRSMWERGWSFIKKAGTIILLSTILVWFTTYFGFVDGTFRMLGEDEIGNSILAAIGNG
                     LAWIFAPLGWGNWQATVASITGLVAKENIVGTMGILYGGGDGTVYQTMAASFTLVS"
     gene            complement(9729..9950)
                     /locus_tag="PLS64_02765"
     CDS             complement(9729..9950)
                     /locus_tag="PLS64_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRL42787.1"
                     /translation="MKTLKQAKVGETVKVVKLHGEGAVKRRIMDMGITKGVEIYIRKV
                     APLGDPIEITVRNYELSLRKADAEMIEVE"
     gene            complement(10036..10248)
                     /locus_tag="PLS64_02770"
     CDS             complement(10036..10248)
                     /locus_tag="PLS64_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRL42788.1"
                     /translation="MMPLMFANTGETNIIKKVGGSPEVKKHLENLGFVAGGEVTVISS
                     LGGNVIVNVKETRVAISEEMARKIMI"
     gene            10540..10911
                     /locus_tag="PLS64_02775"
     CDS             10540..10911
                     /locus_tag="PLS64_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006571805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRL42789.1"
                     /translation="MQLKESGEMYLETIYVLSKKSNYIRSIDVCEEMGYSKPSVSRAV
                     GLLKEGGYLKVGDDGSLILTDLGREIAEKTYERHTVLSKLFVMLGVNPETAAADACKM
                     EHIISDETFAALKNHLENHMQ"
     gene            complement(11119..11718)
                     /locus_tag="PLS64_02780"
     CDS             complement(11119..11718)
                     /locus_tag="PLS64_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL42790.1"
                     /translation="MRKLAVYVHGKGGNATESEHYKPLLPEYDVIGFDYKSETPWDAK
                     QEFTNLFDNYCKNYDYIVLIANSIGAFYSMNAFSDKQIDKAMFISPVVDMENLITNMM
                     KWANVTENELRDKKTISTEFGETLSWEYLCYVREHQISWNVPTSILYGENDNLTSFET
                     INKFAKKINADLTVMNGGEHWFHTAEQVKFLDNWLTKFI"
     gene            complement(11815..13962)
                     /locus_tag="PLS64_02785"
     CDS             complement(11815..13962)
                     /locus_tag="PLS64_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HRL42791.1"
                     /translation="MDYSQILSQQFNIKEEYAKNIIALLDDGNTIPFIARYRKEMHGS
                     MDDQLIREFAEKLEYLRGLDKRRDEIRSLIDAQEKLTDEINLALDKAATLSELEDIYR
                     PYRPKRKTRASIAKEKGLEPLANDILKQEKGFDPSKSAVDYIDEEKGVTSVEDAIQGA
                     MDIIAELVSDNAEIRKRIRNLTNANGVLVSVATDEEKDSVYKNYYDYKEPVKKIAGHR
                     VLAVNRGEKEGFLKVSVETDSEKPLNSIFRAMITDKNQLCSDIIREACTDAYQRLIYP
                     SIEREVRNDLTDTASENAMKVFAVNLKQLLMQPPVKGKTVLALDPGYRTGCKTAVVDS
                     TGKVLDTTVIYPTHSDKKIQESKQTLLRLIKKHHVDIISIGNGTASKETEMFTAETIK
                     EADHPVYYMVVSEAGASVYSASKLAATELPDLDLTLRSAVSIARRLQDPLAELVKIEP
                     KAIGVGQYQHDMPQKKLGETLDGVVEDCVNSVGADLNTASPALLSRVSGLNATVCKNI
                     VAYREENGAFSSRAELKKVPKLGPKAFEQCAGFLRVPESRNPLDNTGVHPESYKSAKE
                     LLGLLEYSDKEIKSGNFSDIQQRVKAKGTKSLADVLGIGLPTLDDIVKELSKPGRDPR
                     DELPAPMLRSDILDIKDLKEGMELKGTVRNVIDFGAFVDIGVHQDGLVHISQICDKYI
                     KHPSEVLKVGDVVNVKVLSVDPEKNRISLTMKF"
     gene            complement(14081..14923)
                     /locus_tag="PLS64_02790"
     CDS             complement(14081..14923)
                     /locus_tag="PLS64_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HRL42792.1"
                     /translation="MDSRFNSPAEIASLVSKSGVTKSMLSLPKMILLGLMAGAFIAFG
                     GAASDVAMHGVADVGLARVVAGVIFPVGLMLVVFTGSELFTGNCLMIIPTLEKKIKPV
                     SMIKNLVTVYISNFVGAMIIDLLIFFSGQLNYSNGGLGAFTIKVALAKTTINPATAVV
                     SGILCNILVCLAIVMATGATDAIGKIFGVFFPICAFVVCGFEHCVANMFYIPTGVMAA
                     MNPEYVAKAQELYGITAQQCQNLANLSGCESLLFVTIGNIIGGMVFVGLPLYFAYIRK
                     KKSA"
     regulatory      15167..15267
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15355..>15606
                     /locus_tag="PLS64_02795"
     CDS             15355..>15606
                     /locus_tag="PLS64_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRL42793.1"
                     /translation="MKTNGKKNALVMCECAIMIALAAVLSFVKILELPYGGSVTAFSI
                     VPIVIISYRHGVKWGLLSGFVFSIIQLIQGASTLSYATSF"
BASE COUNT         4517 a         3470 c         3016 g         4593 t
ORIGIN      
        1 tagcacgctc aaggcaatcc gacacaagct ttctgccgac accttttccc cttgacgatg
       61 aggaaaccgc atagcttgca ttacaaatat gaccgcatct gccgacatta ttcggatgaa
      121 gaatatacat tccgagaatc tcgccgtttt catcggttgc aacgggacaa caagtttgct
      181 ctgcaaaaaa cttttttccg ctttcaaaat caagttcttc agtctgcgga aaggcaactc
      241 cgtcccttac aacttcattc catacggaaa tcataccatt aatatcattt tccccgtatt
      301 ctctcacggt aatactcatt ttcagtcacc tcgggttaat tttagcacta taagttaaaa
      361 ttttcaatcc aaacggctga aatatttact atttttacct ttttaaaatt gcaaattaat
      421 cttgaaattt gtcgaaagat actgtataat ctatttatag aattgaataa gaatgatctt
      481 atcaagagtg actgagggga caggccctat gaagtccggc aacctgcgta tgcaaggtgc
      541 caaatcctgc ggtatttacc gaaagatgag taaacacagc acctttcgga tgaacggtgc
      601 ttattttttt ggaaggagtt ttctaaatgg ctaaacattt atttacatcc gagtctgtaa
      661 cggaaggtca tcccgacaaa gtgtgtgacc agatttccga cgcagttctt gacgaaatcc
      721 tctctcagga taaaaacgct catgttgcct gtgaggttac ggctacaaca ggcatgattc
      781 atgttatggg tgaaatttca accgattgct atgttgacat cgcagatgtt gcaagaaagg
      841 ttgtaaacaa cattggctac aacgatccga agtgcggttt tgacggcaac acctgtgcgg
      901 ttctcacatc aattgacgca cagtcacccg acattgcaat gggcgtaaac gaaagccttg
      961 agcttaaaga cggcgaaagc gataccgaca atcagatcgg tgcaggtgat cagggcatga
     1021 tgttcggtta tgcgtgtgat gaaacacccg aacttatgcc gatgccaatt tctcttgccc
     1081 acaagcttgc aaaacagctt acaaaggtaa gaaaagacgg cactctttca tatctccgtc
     1141 ccgacggcaa gactcaggtt actgttgaat atgacgatga caagattgtc ggcattgaca
     1201 cggttgtaat ttcaacacag catgatgaag atgttacact tgagcagatt agaaaagacc
     1261 ttatcgagta tgttatcaaa ccgattatcc ctgctgagct tatgaacgat aacacaaaga
     1321 ttttcgtaaa cccgacaggc agatttgtaa tcggcggccc ggtcggcgac tcaggtctta
     1381 caggcagaaa gattattgtt gatacctacg gcggattttc tcgtcacggc ggcggtgcat
     1441 tcagcggtaa agatccgaca aaggttgacc gcagtgccgc ttactactca agatatattg
     1501 caaagaacat tgttgccgct aagcttgcca agaagtgcga aattcagctt gcatatgcaa
     1561 tcggtgttgc aaagcctgtt tcaataatgg ttgacacatt cggcacatca aaatattcaa
     1621 acgaagaact ggcaaacgct gttgaaaagg tatttgactg ccgtccgcag tctatcatca
     1681 gacagctcaa gctcacggaa acaaagtatc tcccacttgc cgcttacggt catatgggca
     1741 gagaagaact cggtgttgaa tgggaaaaga caaataaggt tgacgagctt ttaaaggctg
     1801 tcggctaata gaattaaatt gttcgataat ttcgggagta aggtttgtgt acaagcgcaa
     1861 attcttgttc ccgatttttt tattttaaaa aatattgttg ataaattgta caaacaatta
     1921 caatactacg ctcgggcttg atatgttttg tgggcattac aaactatccg cccctacatt
     1981 ctcataacaa catatatttc aaatataaac acaaaaatcc cgattaactt tttacggtta
     2041 atcgggattg taatttttat acggatatta tttttaattc ttaatcagaa ttaaacttct
     2101 gcaataacct caacttcaac aaggacatcc tttggaagct gttttactgc aacacagctt
     2161 cttgccggct ttgatgtgaa atacttgccg taaacctcat tgaaagcacc gaaatcttcc
     2221 atatttttta agaaacaaac tgtcttaaca gccttttcaa gtgaactgcc tgatgcctca
     2281 agaacatttt taaggtttgt gcaaacctgt tctgtctgag cctcaattgt tgcagcctca
     2341 acattgcctg ttgcagggtt aattgcaatc tgaccggatg taaaaacaag accgtttacc
     2401 tttacagcct gtgaatacgg accgatagca tcgggagcat ttttagtata aaccttttcc
     2461 attataatga cctcctgtca atatattaaa aattactgat taaacaagct taaagcctgc
     2521 gtcaacaagt gcatcacgaa tttctttaat ctgagcaaaa tctcttgttt caagaacaac
     2581 acggaggtaa caaccgttta catccgtacc ctctccgcct ctttcgtggt ggatagaaat
     2641 tacatttgcg cccttttcgg caatgatatt tgaaacggca acgagctgac ccggcttatc
     2701 ctgaagttca acacagaaag tctgctgtct gcctgtcata agaagtccac gcttgataac
     2761 tcttgaaagg attgtaacat caatgttacc gcctgataca acgcaaacaa ccttcttgcc
     2821 cttaacaggg agcttgttga acattacagc agccaaagga gctgcgcctg cgccctcggc
     2881 aatcatcttc tgtgactcaa taagcttgag aattgcagat gaaatttcat catcacttac
     2941 cgttacgata tcgtcaacat acttcttaac atattcaaat gtatgctcac ccggttcttt
     3001 aacctgaata ccgtcggcaa ttgtagaaac gctgtcaaga tgaacaggct cgccgttctt
     3061 gattgactca accattgaag gagcgccctc tgcctgaaca ccgtaaacct taatgttagg
     3121 attaaggctc ttgattgcac aggcaacacc cgaaataagt ccgccgccgc cgattgcaca
     3181 tacaacagcg tcaacatcgg gcatttcatt cataatttcg ataccgattg taccctgacc
     3241 tgcaattaca ttttcatcgt caaacgggtg gataaatgta tagttctttt catcacgaag
     3301 tcttaaagcc tcgttataag catcatcata aacacccgga accatacaaa cctcggctcc
     3361 gtagttctta gtagcctcaa cctttgaaat aggtgcaccg tcaggcagac agataagcga
     3421 cttgataccg tactttgtag cagcaagtgc aacaccctga gcgtggttgc ctgcagaaca
     3481 ggcaataaca cctcttgcct tttcttcttc ggtaagctga gaaatcttgt aacctgaacc
     3541 tcttacctta aatgaacctg taatctgaag attttcgggt ttgagataaa catcacaatc
     3601 atcacaaata ccccttgcct gaacgatatt agtctgtctg ataacatcct tcaaaacata
     3661 tgatgcgtga tagattttgt ctaatgtaag cataccaatt acttcctttt ctatatgtat
     3721 ccactgctta ttttaataca acaaggcttt aaaatcaagt ttttttagaa taaacaaagg
     3781 gaatttttgc aggatttttt aaagtcttat tcataattcg caaacagaat ataaaataat
     3841 aataacgaac aaataaaaac acctgcttac aagcgtaaac aggcgtttct atgaaattat
     3901 gaaattggta ttaactaatt gaatacttac agcctaagtt aagcaagtga tgcgccaagc
     3961 tttttgcaag cctcaagggc gtcgtcatcg ggagcatcgt tacagataac gctttcgcaa
     4021 ataaggtttg caccgtcacc cttacaggtg tcctcccagt ttctcatcca ttcgccgtca
     4081 ccccagccgt atgaaccgaa gagggcaatg cttttgccgt ttaatgcact ttcacattca
     4141 ttgaaaacag gctcaaactc gctgtcctca agaacctccg cccccattgc aggacaaccg
     4201 aatgcaatgc cgtcaaaatc ggcaaccgat gaagaatcaa aatcgtttac atttgcaaga
     4261 gtaacttctg cgcctgcgga ctttgcaccc tcggcaacgg cttccgccat ctgctctgtg
     4321 ttacctgtac cgctccagaa tactactgct actttactca tattatatac tcacctttca
     4381 gtttttgtac ttgattacac tttaagtcag ttacacgcaa ccgtcagttt ctgaataggt
     4441 gtgccctgta accacttaga gtaatagatg tcagaacatt ttttgaattt ggcgaactct
     4501 ttggctgctt tttcttcatt accgtaaacc ttccagcaac ctgaatattt acagcaattt
     4561 cctttatgaa gaataaagcc acggacatct tcgggaggat aaccgaggaa cagtccgatt
     4621 tcgtgcggga actcggttga gtctttaagt cggtggcgaa gctcccttat acagcgtgcg
     4681 gcatcgtgtg aatgataccc ctgcgactca agcagcttgc acgcatattc atcggacaaa
     4741 tcaacagcaa gcttggacgg gcgaaaaata tagattaaag cctttccgtt gctgaacttc
     4801 agtggcagaa cttttattcc ctttttgcag aacattttat tcagctttct cacatcacat
     4861 tcgcactcaa gccgtgatga gtacggacag ttaaaaaggt tagccgtttt tattcctgca
     4921 agcgttgggg aacaatgctt gataattaaa tcttctgaca tcttttcctc taaataattg
     4981 aatgaatgat ttgaatagaa ttgaataaat acaccacagt gattatccga taattgtttt
     5041 gcagttagct tttgctaact taattccata agcaagacta ttttgttgtc tttatcttac
     5101 agttaaaata taccatcaat caatgttgtt atcggctctt aaaaggtttt ctacgctcca
     5161 aacggacaga taatttcagc aaagatttag ggacagcaaa cgctgtccct gaaaggagaa
     5221 gagatattat gaataattac ccggcaaagt aatcatcaaa tactctgcgg atatttccgc
     5281 agtctgtcag aaaagaatat aggcgtatct tttcgacaat gttattatag cactttacaa
     5341 agtcttttta ctgctcttta cggacatata aggctctgtt cagacgagcg attttctgta
     5401 ctcattcggc gatttgttca tcacccttct gaaagcaccc gcaaacttac tgccgttgtt
     5461 gtagccgaca attcctgcaa tttcaagcac acttttgtcg gtgtttttca aaagcgaggc
     5521 ggccttttgc attttacagt tcgttatgta gccgttaatc gtgtcgccgt agacaccctt
     5581 aaaacagatt ttgagagaag ttgaagagat gccgagcata tcggcaagct ccgtaattgt
     5641 tatatgctcg tcaaggtgag caagcagata tttttttgcc tcttttgcgg cggtaacctg
     5701 tgagcgtgaa aaatatcgtc tttcctcact ctcacccttg tattcaagac cgctcaaaaa
     5761 caaaagaagt tcaagcacct taaccttgtg ataacccttt cggatatttt cgggaacgga
     5821 atagagttcc gaaaaaatat gctcaatgct gatattttca cgcataattg agtacggctt
     5881 ttcggaacag aatttcattt caagctcttc ggggctgaca aaaacatcgt caagaataca
     5941 cgaaaaacac cttggcacac ggttcatatc aattgcaacc gacacaccgt ggtagtggct
     6001 cagcggacaa tatctgtgtc ggatgttctc cgtattcttc tgtatcgaca aatcacccga
     6061 cgcaaggtaa agatattcgc cgtttttgta ttcaaactca attctgccct cctgacaatg
     6121 cgtgatgatg agcatttctc ttccctttgc aagattaatg tcacaacttt gcatatgtac
     6181 atcgtaatac acaagctcaa tgcctgaaaa cacaggatac acggtgacct cgcaatcgcc
     6241 cgtgctgtcc tgtgaacggt atatgctgaa cgattcgcag tgttcaacag tctgtatcat
     6301 accgtatgga ttgtcaattt tctgcaatac tgccataagc tcctaaaacc cttattccgc
     6361 cttcataacg gtgtatgcac ctatatcgtt aactgtcttt ttgaggactt tatcctcaat
     6421 ttctcttttc attctgaccg taaccctgcc ggaagaaaca tcggcttccg cccacacacc
     6481 gtcaattgaa ttgagtgcgt tttctactct tgcggaacag tttttacaga ccattccgtc
     6541 aacaacaaga gttttttcat agggataatg tgacttatcc ttatcggcaa ccttgttttt
     6601 cttaacgctt tcgtcacttg caccgcaaca tccgcttgca agctttttcc tgtaacttct
     6661 gattacgaga accacaaacg caatgaatgc cacggcaagc aacccgtaaa aaacatactc
     6721 catatttatc tctcctttta ttttttacga ttcagaacag ttctgaatac actttacacc
     6781 gtaaaatttc ttcaacacca aattcataat ttcaataaca aacaagaagg ggtacagctt
     6841 tttagctgta ccccctacaa aacatttact tatgattatg gcagtcaccg tgacacgcac
     6901 agtgcgcaca gtttgcaccg caggaggatt tgccctcttt tttgtctttt accatacggg
     6961 caaccacaaa agcaatcacg gcaataacaa ttaatgcaac aatgattgtg ccgatattag
     7021 ccgctaacca ttccaacata tttattcctc ccattgttaa tcaataaatt tattacttct
     7081 tagccgactt aactttaaga gttgtagcct ctttgtaagg tcttacgagc atataaatca
     7141 taaatgcaaa tactgcaacg gcaaagataa gaccgattac attaagaccg cctgcaaaga
     7201 ttgaaccgaa ctggaatacc ataaatgcaa ttacataagc aaatacacac tggtaaccga
     7261 ttgcgaacca gaaccacttg atgttgttca tctctctctt gatagcaccc atagcggcaa
     7321 agcaaggagc gcagagaagg ttgaagagaa ggaacgagaa accgctgagt cctgtgaatg
     7381 cggtgccgat ttccgtccat ccgcccggat aaaggnnnnn nnnnncggaa acaagtgtga
     7441 aggaagctgc cattgtctga tatactgtac catcgccgcc accataaagg atacccattg
     7501 taccaacgat gttttcctta gcaacaagac ctgtgatgga tgcaacggtt gcctgccagt
     7561 tgccccagcc gagcggtgca aaaatccatg caagaccgtt accgattgcg gcaagaattg
     7621 agtttccgat ttcgtcctca ccgagcatac ggaatgtacc gtcaacaaat ccgaagtatg
     7681 ttgtgaacca aacaaggatt gtagaaagga gaataattgt gccggctttt ttgatgaatg
     7741 accagccacg ctcccacatt gaacggagaa ggttgcctac tgtcggaaga tgataagccg
     7801 gaagctccat tacaaacgga gcgggatcgc ctgagaacat ctttgtcttt ttaagcataa
     7861 tgcctgaaac tacgattgca gccataccga tgaagtaagc cgatgttgca atccacggtg
     7921 aaccaccgaa gatagcacct gcaatcatag cgatgaacgg ctgtttagca ccgcagggaa
     7981 tgaatgttgt tgtcataata gtcatacggc ggtcacgctc attctcgatt gtacgggacg
     8041 ccatgatacc cggaacaccg cagcctgtgc cgacaagcat tgggataaac gacttacccg
     8101 aaagaccgaa cttacgaaaa atacggtcaa gtacgaatgc gatacgagcc atataaccgc
     8161 agctttcgag gaaagcaagg agaatgaaga gtacaagcat ctgcggaaca aagccgagaa
     8221 ccgcacctac accggctacg ataccgtcaa ggataagacc gttgagccag tctgcacagt
     8281 tgattgcatc aaggccgttg ccgataagaa caggaatacc gggaacccag attccgtatg
     8341 ttgcggcatc gggagcaccg taaccctctt tataatctgt gttgaggtag tttgaaacag
     8401 ccttttcaag atcagatttc ttagtatcgg gagtttctgt aatttcaaga gtttcttcat
     8461 cctgaacaga atatgtaacg cttgcgtctg aaggtgtgag atttacgagt tctgcaagag
     8521 cggccttagc ggcgtcttcg gaataatttt tagattcaag gtcaacagct tcggcaatgt
     8581 cgtcatttcc gtactgagca ttgaatgcct cgataattgc atctgaatca ccgtaggttt
     8641 cttcagcctc ggcagcctgt gatgagccga taccgaaaag gtgccagccg tcaccgaaaa
     8701 gaccgtcatt tgcccagtca gttgcggcag tacctactgt ctgcattgag atgtagtaaa
     8761 caaggaacat aataaccgca aaaatcggaa gaccgagcca acggttggtt acaaccttat
     8821 caatcttgtc cgatgttgta agcttaccct tgtttttctt cttataacag ctcttgatga
     8881 ttgaggcaat gtagatgtat ctctcattag tgatgatact ctctgcgtca tcatcaagct
     8941 ctttttcagc cgcctgaata tctttttcaa tgtggtcaat aacattctgc ggaatgtcaa
     9001 gctttgcaag aaccttgtcg tcacgctcaa aaatcttgat agcgtaccaa cgctgctgtt
     9061 cctcgggcat attgtgaaca gccgcctctt caatgtgagc gattgcgtgt tcaacagcac
     9121 cgttaaagct gtgcatgggg attgtctttg ttgagcctgc ggctttgatt gcggcctcgg
     9181 cagcttccat aatgcctgtg cctttaagag ccgaaatttc cataacctta caaccaagct
     9241 gtcttgaaag ctcatcaatg ttgattgaat cgccgttctt cttaacgagg tcaatcatat
     9301 tgattgccac aacaacaggg ataccgagtt ctgtaagctg tgttgtgagg taaaggtttc
     9361 tttcaaggtt agtaccgtca ataaggttga gaatcgcatc ggggcgttca cctacaagat
     9421 agttacgggc aacaacctct tcaagagtat aaggtgaaag tgaatagata ccgggcaagt
     9481 ccatgattgt aacatccttg tgctttttga gcttaccctc ttttttctca acagttacgc
     9541 cgggccagtt accgacaaac tgattggaac ccgtgagtgc gttaaacaaa gttgtcttac
     9601 cgcagttagg gttacctgca agagcaattt taatttccat atatcagtta tcctctctta
     9661 gattagcaaa agctaaccgt aatgttataa aaatacggga tttctcccgt aggatttata
     9721 agtataaatt actcaacttc aatcatttca gcatcggctt ttctgagcga aagttcataa
     9781 tttcttacag taatttcaat cggatctccg agcggagcaa cttttctgat gtaaatttca
     9841 acaccctttg tgatgcccat atccataatt ctgcgtttga cagcgccctc accgtgaagc
     9901 ttaacaacct ttacggtttc acctactttt gcctgtttta aagttttcat aacagtttcc
     9961 tccatctaaa attgagcata aagccgtata aaaacacctc tcggaacaac tccgccgtgt
    10021 gtttgttaca tagaatcaaa tcataatttt acgagccatt tcttcgctga ttgcaactcg
    10081 tgtttccttt acgttaacaa taacatttcc gccgagtgac gaaataacgg taacctctcc
    10141 gccggcaaca aaaccgagat tttcaaggtg ctttttaacc tcgggagaac ctccgacttt
    10201 ctttataata ttggtttcac ctgtgttagc aaacatcaaa ggcatcataa aatcaatctc
    10261 ctttaagtta gcagatacta acaatatttg ttaatcaaag gttagctctc gctaaccgat
    10321 tatatggtac tataaaactc gaaatatgtc aataggcttg cgaaaaaatt ttattttatt
    10381 ttgaattttg gcaaattgaa tatttcacgc ctgtaattcg gatattattt ttgttgattt
    10441 tggtaaatcg ttttttcata attattgaaa gcacaattaa ttttattgat tttttgttca
    10501 tatcatgata tgattagaca atgtaaagga gtgcagacta tgcaattaaa agaatcaggt
    10561 gaaatgtacc ttgaaacaat atatgttctt tcaaaaaaga gcaattatat aagatcaatt
    10621 gatgtatgtg aagaaatggg atattcaaag ccgagcgtca gccgtgccgt tggtcttctc
    10681 aaagagggcg gttatcttaa agtcggcgat gacggctcgc ttattctcac cgacctcggc
    10741 agggaaattg cagaaaaaac atatgaaaga cacaccgttc tttcaaagct ctttgttatg
    10801 ctcggagtta atcccgaaac tgccgccgca gatgcctgca aaatggaaca cattatcagc
    10861 gacgaaacct ttgccgcatt gaaaaatcac cttgaaaatc atatgcagta aaaggctgaa
    10921 taattaccga atattacaca caaaaggctg acgatttctc gtcagccttt gtttttactc
    10981 aactgctttc aataaggcga tacacttttt cgattgattc aacaattcga cacgcttaaa
    11041 taaaacgcaa aaataacaat agtacaaatt attgcaactg cctttttcat aagcacacct
    11101 cgttattata aaatatcatc aaatgaactt tgttagccaa ttatccaaga atttcacctg
    11161 ttcagctgta tgaaaccaat gttcaccgcc attcataaca gtaaggtcag catttatctt
    11221 ctttgcaaat ttattgattg tttcaaacga tgtaaggtta tcattttcac cgtacaaaat
    11281 tgaggtggga acattccaag aaatttggtg ttcacgaaca tagcaaagat attcccaaga
    11341 taaagtttca ccaaattcag tagagattgt tttcttatca cgaagttcat tctctgttac
    11401 attcgcccat ttcatcatat tggtaatgag gttttccata tctacaacag gcgaaataaa
    11461 catagctttg tcaatctgtt tatctgaaaa ggcattcata gaataaaatg cacctatgct
    11521 attcgcaatc agaactatat aatcataatt tttgcaataa ttatcgaaca gatttgtaaa
    11581 ttcctgtttt gcatcccacg gtgtttctga tttatagtca aaacctataa catcatattc
    11641 aggtagcaaa ggtttgtaat gctccgattc tgtagcatta ccaccctttc cgtgtacata
    11701 tacagccagt ttacgcattt aaaattctcc ttttgcggaa caatatcaac agttgcggaa
    11761 taatgcaaag gtgatttgcg gaacaataca gttatctttt ttgctttatt cgcattaaaa
    11821 tttcattgtg agagaaattc tgttcttttc gggatcaact gaaagaacct ttacatttac
    11881 aacatcaccg actttgagaa cttcgctcgg gtgcttgatg tacttatcgc aaatctgcga
    11941 aatatgaaca agtccgtcct gatgaacacc gatatcgaca aatgcgccga agtcgattac
    12001 atttctgacc gtgcctttaa gctccattcc ctctttcaaa tccttgatgt cgagaatatc
    12061 acttctgagc attggtgcag gcagttcgtc acggggatct ctgcccggtt ttgagagttc
    12121 ctttacaata tcgtcaaggg ttggaagtcc gattccgaga acatcggcaa gtgactttgt
    12181 tccctttgct tttactctct gctgaatatc ggagaagttg cctgatttta tttctttgtc
    12241 agaatattca agaagtccta aaagttcttt tgcactcttg tagctttcgg ggtgaacgcc
    12301 tgtgttatca agcggatttc tgctttcggg aacacgcaaa aatcctgcac actgctcaaa
    12361 cgctttaggt ccgagtttcg gaactttctt gagttctgct cttgatgaga acgcaccgtt
    12421 ttcttcacgg taagcaacaa tattcttgca cacggttgcg ttaagtcccg acactctcga
    12481 caaaagtgca ggcgatgccg tattaaggtc tgcaccaacc gagtttacgc agtcctctac
    12541 aacaccgtca agtgtttcac cgagtttttt ctgcggcata tcgtgctgat actgaccgac
    12601 accgattgcc ttaggctcaa tcttaacaag ctctgcaaga ggatcctgaa gtcgtcttgc
    12661 aattgacacg gcacttctga gtgtgaggtc aaggtcggga agttctgttg cggcaagctt
    12721 tgatgccgaa taaaccgacg cacctgcctc gctgactacc atataataaa cggggtggtc
    12781 agcctctttg attgtttcgg ctgtgaacat ctcggtttcc ttgcttgcag taccgttgcc
    12841 gattgaaata atatcaacat gatgcttttt gatgaggcgg agcaaggtct gctttgattc
    12901 ctgaattttt ttgtctgaat gtgtaggata aattacggtt gtatcaagca ccttgcctgt
    12961 gctgtcaaca acggcagtct tacagcctgt gcgatatccc ggatcaagtg caagcacggt
    13021 tttgcccttt acaggcggct gcataagcag ctgcttgagg tttacggcaa aaaccttcat
    13081 agcattttcg gaggcggtat ctgtaaggtc gtttctgacc tcacgctcaa ttgagggata
    13141 aataagtcgc tgataagcgt ctgtgcaagc ctcacggata atgtctgaac aaagctgatt
    13201 tttgtctgta atcattgctc tgaaaattga attaagcggt ttttcgctgt cggtttcaac
    13261 gctgactttg aggaatccct ctttttcgcc acgattcacg gcaagcactc tgtgacctgc
    13321 aatcttttta acaggctctt tgtagtcata ataatttttg taaacgctgt ccttttcttc
    13381 atctgttgca acagacacaa gcacaccgtt tgcgtttgta agatttctta ttctcttacg
    13441 gatttccgca ttatccgaca caagttctgc aatgatatcc attgcaccct gaattgcatc
    13501 ttcaacggag gtaactccct tttcttcatc aatataatca actgccgact ttgacggatc
    13561 aaaacctttt tcctgcttta aaatatcgtt agcaagcggt tcaagtccct tttcctttgc
    13621 aattgacgcc cttgtctttc tctttggacg gtacgggcgg taaatatcct caagttcgct
    13681 tagcgtggca gccttgtcaa gtgcaaggtt gatttcatcg gtaagctttt cctgtgcgtc
    13741 aatgagtgaa cggatttcat ctctgcgctt gtcaagtcct cttaaatatt caagcttttc
    13801 ggcaaattca cgaatcaact ggtcatccat tgaaccgtgc atttcctttc tgtatcgtgc
    13861 aataaaagga atagtgttgc cgtcatcaag caatgcgatg atgttcttgg catactcctc
    13921 tttaatatta aactgctgtg aaagaatctg tgaataatcc attatatcct ccgtttttgt
    13981 tttgtaaggc ttattataac atacttttaa ataatttgca ctaaaaagaa aacgagcagt
    14041 caaaacgact gctctcatga attatttatg tttatgtatt ttaagcactt ttctttttgc
    14101 gaatataggc aaaatagagc ggaagtccta caaagaccat accgccgatt atgttgccga
    14161 ttgtaacgaa cagcagagat tcacagcccg aaagatttgc aagattctga cattgctgtg
    14221 ccgtaatacc gtaaagctcc tgagcttttg caacatattc gggattcatt gccgccataa
    14281 cacctgtcgg aatatagaac atatttgcaa cgcagtgttc aaatccgcag actacaaatg
    14341 cgcaaatcgg gaaaaataca ccgaaaatct tacctattgc atctgttgcg cctgttgcca
    14401 taacgattgc aaggcacacg agaatgttac aaagtatgcc gctgacaacc gctgttgcag
    14461 gatttattgt tgttttggca agtgcaacct tgattgtaaa agcaccgagt ccgccgtttg
    14521 aataattgag ctgtcccgaa aaaaatatca gcaaatcaat aatcattgca ccgacaaaat
    14581 ttgaaatata aaccgtaaca agattcttta tcattgaaac aggcttgatt ttcttttcaa
    14641 gcgtcggaat aatcataagg cagttacctg taaagagttc acttcccgta aacacaacaa
    14701 gcataagtcc tacagggaag attacgcctg caaccactct tgcaagcccc acatcggcaa
    14761 ctccgtgcat tgcaacatcg cttgccgcac cgccgaatgc gataaatgcg cctgccataa
    14821 gtccgagcaa aatcattttc ggcagagaaa gcattgattt tgttacgcct gattttgaca
    14881 ccagcgacgc aatttcagcc ggtgaattaa atctgctgtc cattaaaaaa ccttcttcaa
    14941 ttagtttatc aattgttgca aaatttactg ccattcggca ataaaccgca aaatcacagg
    15001 atacatatta tcacaaaagc aaagtgtttg tcaattaata tgcgattatc tgtaacattg
    15061 cacacaaaag gctttacaaa ttgcaaaata cagtttataa taatcctgtg agtgttctcc
    15121 gccgtttaaa cgggtgagta cattcttaag ttaaggtgag aataatcaca tacaaaacgg
    15181 gagctgttac cggctgagag gaaattcgat ttcgaccgta aacctgattt ggataatgcc
    15241 aacgaaggga catatcaagc ttgaagtgta cgcacttgga gtatgtcttt ttggcatact
    15301 ccttttttgt tgtgtttttt ctccgagaga attttatttt aaggagtagt tttaatgaaa
    15361 acgaacggta agaaaaatgc acttgttatg tgcgaatgtg caattatgat tgcacttgcg
    15421 gcagttttaa gctttgtaaa aatccttgaa cttccctacg gcggcagtgt taccgcattc
    15481 agcattgttc cgattgtaat tatctcatat cgccacggtg taaaatgggg attgctttcg
    15541 ggatttgtat tcagcattat tcagcttatt cagggagctt caaccctttc atacgcaact
    15601 tcattc
//
LOCUS       DAOMVE010000028        15437 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_801_length_15437_cov_3.094266, whole genome shotgun sequence.
ACCESSION   DAOMVE010000028 DAOMVE010000000
VERSION     DAOMVE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 15437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15437
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_801_length_15437_cov_3.094266"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..951
                     /gene="rpsB"
                     /locus_tag="PLS64_02800"
     CDS             229..951
                     /gene="rpsB"
                     /locus_tag="PLS64_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRL42794.1"
                     /translation="MAVVSMKQLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KSVKKLEEAYNFVRELSAEGKSVLFVGTKKQAQDSVKEEAERAGAYYVNARWLGGMLT
                     NFATIRRRIARLKQLRAMQEDGTFDLLPKKEVIKLNLEIEKLEKFMGGIKDMTEMPGA
                     LFIVDPRKERIAVSEAKKLNIPIVAIVDTNCDPDEIDYVIPGNDDAIRAVKLIAGAMA
                     NAVIEGREGRMGAAAVEEETAE"
     gene            1008..1919
                     /gene="tsf"
                     /locus_tag="PLS64_02805"
     CDS             1008..1919
                     /gene="tsf"
                     /locus_tag="PLS64_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRL42795.1"
                     /translation="MAAFTAQDVKTLREKTGCGMMDCKKALTNADGDMDKAVDFLREQ
                     GLAKQAKKAGRIAAEGVAYACTNDDLSVGVVVEVNAETDFVAKNDSFMDFVKACANTI
                     IEQNPADVEALLTLKATGSEQTIAEMLQEKVLTIGENIQIRRFERMEGACVAYVHAGG
                     KIGVLVNFDTDLAAKPEFVAYGKDVAMQIAALNTPYLNESEVPAEIIEHEKEVMRQQV
                     INEGKPEQIADKIVMGKIGKFYKENCLLDQEFVKDNKINIKQYTQNTAKELGGSIEIK
                     KFVRFEKGEGIEKRQDDFAAEVASMVK"
     gene            2253..2795
                     /gene="infC"
                     /locus_tag="PLS64_02810"
     CDS             2253..2795
                     /gene="infC"
                     /locus_tag="PLS64_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRL42796.1"
                     /translation="MRLWRCLPISKKEIQINEEIRDKELRIIGSDGQQLGIMSAKEAL
                     DLAEQKNLDLVKIAPQSNPPVCKIMDYGKYRFEQAKREKEARKNQRVVDIKEVRLSLN
                     IDTHDFNTKLNNALKFIKHGDKVKVSIRFRGREMGHPEIGLETMKRFADACSETAVVE
                     KPAKLEGRNMLMFLAPKTNK"
     gene            2819..3016
                     /gene="rpmI"
                     /locus_tag="PLS64_02815"
     CDS             2819..3016
                     /gene="rpmI"
                     /locus_tag="PLS64_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRL42797.1"
                     /translation="MPKIKTHSGAKKRFKLSKNGKVIRAHANKSHILNKKTTKRKRGL
                     RQTVAADKTNTAQIKRLIPYK"
     gene            3059..3409
                     /gene="rplT"
                     /locus_tag="PLS64_02820"
     CDS             3059..3409
                     /gene="rplT"
                     /locus_tag="PLS64_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRL42798.1"
                     /translation="MARVKGAMMTRKRRKKTLKLAKGYWGAKSRHFKMAKQAVMKSGN
                     YAYIGRKQRKRDFRRLWITRISAACKANGTNYSTFMNGLKKAGIALNRKMLSEIAISD
                     PAAFTSLVEQAKNA"
     gene            3482..4264
                     /locus_tag="PLS64_02825"
     CDS             3482..4264
                     /locus_tag="PLS64_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRL42799.1"
                     /translation="MTILSSKDNSNIKNAVKLRKSAKYRRESGLFIAEGVRICMDAML
                     SKAHIDTFFASEKASEKYADDFSNLQKYSDKTFIVSDKIFNQMCDTESPQGFLCVIKA
                     LDKTACFDKIKTGDKFLALDNLQDPGNLGTILRTAEALNVTGIVLSADCCDIYSPKVV
                     RGSMGAVFRLPFVVSDTISGYLNAHSELESYAAVVSASADKITTTPFVSPCIVAVGNE
                     GNGLKEETISACKHKITIPMEGRAESLNAAVAASIIMWEMMK"
     gene            4246..5520
                     /gene="dprA"
                     /locus_tag="PLS64_02830"
     CDS             4246..5520
                     /gene="dprA"
                     /locus_tag="PLS64_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRL42800.1"
                     /translation="MGNDEMISRNACYWIWITLSIGYNNPKVKRISEMYSDVSAFYYG
                     GISEWRLCGIFSQKDIERFSSTGLDDAKKIVDRCIECNYSILCIDDELFPKCLYNIEC
                     PPALIYVNGVMPDIDNTFSIGIVGTRRATKYGIENSYRFAYALSKYGTIIVSGGALGV
                     DGASHRGALATDGITICVRGCGLNCSYLRENSDIRSTIPKRGAVITEYPPDETPRNYY
                     FPARNRIIAALSDGLLVMEAGKKSGSLITANLAAEQGKTIFALLGNNSPQNEGSNALI
                     KEGLAIPVTDFMDILCEFDNLYATTDDEFDIDNISLADTGNFPVKGIRKQAPARIYVN
                     KQNDQQPAKTAVPYVSEKSETVVQKPVHKENLNLPKTAQDVYEYIGNEPVHIDKISAD
                     LKIPVFKVLTALTMLEMKDLVSALQGRNYILK"
     gene            5602..7692
                     /gene="topA"
                     /locus_tag="PLS64_02835"
     CDS             5602..7692
                     /gene="topA"
                     /locus_tag="PLS64_02835"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRL42801.1"
                     /translation="MSDLVIVESPAKAKTIKKYLGSGYEVVASMGHVRDLPKSRLSVD
                     IKKNFAPKYEIIKGKEKLVDDLKSAAEKSEHIYLATDPDREGEAISWHLAYILGLPTD
                     ETNRVEFNEITKSGVTNGMSNPRSINIDLVNAQQARRILDRLVGYKLSPFISQKIRRG
                     LSAGRVQSVAVRIIVDREEEIRKFKPEEYWSIDAKFIPKGSRKSFSASLYSDENGKIK
                     ITNGEQADKIEADLQNAEYVITKVKNGTRKKSPAPPFITSTLQQEASRKLGFQSRRTM
                     KVAQELYEGVDIEGMGATGLITYMRTDSLRISNVAIEEVTEYIKNSYGEKFLPPKPRF
                     FKSRSNAQDGHEAIRPSMPSLTPDSVKASLTSDQYKLYKLIWNRFTASQMADCIQKTT
                     QADIVANGYTFKASGYRVDFEGFTTLYVESKDVEEEKTTQLPPLEKDMIVRCKELKKN
                     QHFTQPPARYTEASLIKALEEYGIGRPSTYAATITTITSHEYVKREGKSLVPTELGEV
                     TTNLMKERFPKIVNVKFTNQMEEDLDKVEKGTEEWHALLDNFYGDFDKTLKKAKADME
                     GVKLHLKEDETDIICDKCGRQMVVKVGRYGKFIACPGYPECKNIIKYVEKTGVKCPKC
                     GGDVIIKRTKTKRIFYGCSNYPECDFVSWNEPTNEKCPQCGGILFKKKGKKPTLFCAT
                     EGCGYTKTADTDDK"
     gene            7693..9000
                     /gene="trmFO"
                     /locus_tag="PLS64_02840"
     CDS             7693..9000
                     /gene="trmFO"
                     /locus_tag="PLS64_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HRL42802.1"
                     /translation="MHINVIGAGLAGCEAAMQIASHGIKVHLYEMKPNKKTPAHHTEK
                     YAELVCSNSLKAMRVESAAGLLKEEMRRLDSFLMKCADACKVPAGGALAVDRDIFSSL
                     ATEGIKSCELIEVYEEEVCEIPKDEITVVASGPLTSEPLAEYIRGMFGSSLSFFDAAA
                     PIVTAESIDMEYAFCASRYDKGDGDDYINCPMNKEEYETFYNALISAERAPLHDCDVS
                     NPKVYEGCMPVEVMAQRGEGTLRFGPMKPVGLVNPKTGHRPWAVLQLRKENKAGNMYN
                     LVGFQTNLKFPEQKRVFSLIPALHNADFVRYGVMHRNTFLDSPRILNSDFSVKDNHSL
                     FFAGQITGVEGYMESAASGIMAGINACRVAEGKSTITLPNDTMIGALSAYISDSSVKK
                     FQPMGANFGVLPELENRPRDKKERGAVYSARALKSLDNYLKDI"
     gene            9011..9991
                     /gene="plsX"
                     /locus_tag="PLS64_02845"
     CDS             9011..9991
                     /gene="plsX"
                     /locus_tag="PLS64_02845"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRL42803.1"
                     /translation="MNIIVDAFGGDNAPLEIIKGCVEAVAEYNINVTLTGDETKIKNV
                     FSENSLSMNNIDICHCTQKITMEDSAESVLKEKKDSSMAVGLRLLNEGKGDAFVSAGN
                     SGALCVGATLAIKRIKGIKRPAFAPVMPSESGFFMLMDGGANVECRPEMLYQFAVMGS
                     IYMEKVMGIKNPRVGLANVGAEEHKGTELYRNTHELLQNSNLNFIGNVEGRDIPNGVC
                     DVVVCDGFTGNLILKTYEGVALVMMNQIKNMFMGSVKGKLAAGLVMKDLKNMKTHFDY
                     NRYGGAPILGASKPVFKAHGSAKAVTVKNAIRLSVEYVKANAIEAISSSL"
     gene            10026..10703
                     /gene="rnc"
                     /locus_tag="PLS64_02850"
     CDS             10026..10703
                     /gene="rnc"
                     /locus_tag="PLS64_02850"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRL42804.1"
                     /translation="MEDLQKRIGYTFKNTDLLTEALTHSSYANEHKAKHIKYNERLEF
                     LGDAVLSIVVSDYIFKHCPELPEGELTKLRASLVCEKTLFEFAKQIELGKYLVLSKGE
                     RNNGGAERPSILSDAFEALIAAIYIDGGIDPASKHILNFVIPAIKNSKKKKLNDYKTA
                     LQEIIQKNPGEQLEYVLVGESGPDHNKHFVVEVHLNSNVIGKGGGRSKKEAEQQAARE
                     ALELMGY"
     gene            10726..11691
                     /locus_tag="PLS64_02855"
     CDS             10726..11691
                     /locus_tag="PLS64_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRL42805.1"
                     /translation="MPHNGCPHMCSFCNQNKITGQAYQPTPLDVEQTLGKAASDLGER
                     TKNAEIAFFGGSFTAIDRTYMLSLLDATKKFRDKFCGIRISTRPDYIDKEILDILKSY
                     GVTSIELGAQSMSDDVLLANDRGHSAESVFKSSELIKSYGFSLGLQMMTGLYKSDIQK
                     DLYTAETFVKINPDTVRIYPTVIMKDTKLAELYLNGEYTPYSLEKSVNLCSKLIELFE
                     KNNIKIIRLGLHYSDSLVNNSYGDNYHPAFKELCENKLFYDKFIEKAKDFPDLNEIKT
                     VVINSKSLSKFLGQKRSNVKKLNELGYNFSVCFDDSLQKYELYLK"
     gene            11702..15277
                     /gene="smc"
                     /locus_tag="PLS64_02860"
     CDS             11702..15277
                     /gene="smc"
                     /locus_tag="PLS64_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRL42806.1"
                     /translation="MRLKSLEIQGFKTFPDKTKLSFEQGITSVVGPNGSGKSNISDAI
                     RWVLGEQSPKSLRCAKMEDVVFNGTDKRKRQGYAEVTLNIDNKDRALDFNGDDIAVTR
                     RYYRSGESEYLINKAAVRLKDINELFMDTGLGRDGYSMIGQGKIDSIVSSKGEDRREI
                     FEEAAGISRYRYRKIDAERKLKSTEDNLLRLRDIVTELEERVGPLEKQSEKAQKFLEY
                     SEEKRGLEIALWLLTLDRSQNELKVQDEKISIAKSQYDDAETALEQIQTESEEIYIRN
                     GEYSSKIENIRTEIADIESQISEKRSLVSVAENDILHNNENIDRIKNDIAQFDVNADE
                     LVKRIADKEKEISELDADIIAKKKRYSELSEQLNEINTTSGKSSDVLQEITAELSVLT
                     SKSADARVTEMTAESTVSELNSRIESLISSNDEKKTQSEELADISKDYDNKIGVAEEK
                     IESLKNTIDGLQLKLSSKEKKKDELREESNKLMLDVKEHMIKISFLENLERNLEGFSK
                     SVKTVVNAQSHGKVHGICGPVSRVISVPKEYGVAIETALGSAMQNIVTETDEDAKRAI
                     RYLKSVDGGRATFLPLNTIKPRELRENGLEDCYGFVGVAANLCSCDNKYNGILYSLLG
                     KIVIAEDLNCATTIAKKYSYRFKIVTLDGQVVNAGGSLTGGSLNRNTGLLSRANEIET
                     LKKQTTVLEQKSKDAENRSIEISREYSAFEAQLLGSRADLSNLRQDLVRLEAEKRACD
                     NELANAGASVENSMNEITNCRRRIETLEKDKNSAKQLLVELNKKILAAEEKVNELTGS
                     RAELTKKREEISLQLQNLRLEIVSSQKDIDALNSDIVFAKSTGNENLERKAELNQEIE
                     RIKSVILEKTELINSTNAEIEKLIANQNERSKAIEKINADREQLEKRSAEIRTVERDK
                     TSERETSGRELARLEERKINIQKQYDDIISKLWEEYELTRREAEDCAVEIDDSKQAQR
                     RLNELKQKIRSLGNVNVSAIEEYKEVSERYEFMSAQVNDVEKSKKEIEKLITDLTKQM
                     KEVFVESFDQINKNFTYTFKELFGGGTASLSLADPENILTSGIDILVHPPGKIVINLE
                     ALSGGEKALVAIALYFAIMKVRPAPFCVMDEIEAALDDVNVYRFAAYLRRMTDNTQFI
                     LITHRRGTMEEADVLYGVTMQDEGISKLLELRSTEVAEKLGIDSK"
     gene            15297..>15437
                     /locus_tag="PLS64_02865"
     CDS             15297..>15437
                     /locus_tag="PLS64_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRL42807.1"
                     /translation="MGFFKKIKEGLKKTRDAVVGQIDSMLKSFTKIDDELFEELEELL
                     VMG"
BASE COUNT         5060 a         2713 c         3445 g         4219 t
ORIGIN      
        1 gtggtattga tcggagtttc cgaatacaat ttaatattta agaaaatatc aggtgttttt
       61 gaaaaaactt tttcgaaaac acttgatttt ttttactttt atggtataat atacaggcaa
      121 ttcgcacgct gatgcttgtc aacagggtgg cagattcata gaatttgctt attgcgttga
      181 taaaaacaaa ggcacagcgg aggttttaac ctaaggagga tttttattat ggcagtagtt
      241 tcaatgaaac agcttcttga agcaggtgtt cactttggtc accagacaag aagatggaac
      301 cctaagatgg ctgagtacat ttttactgag cgtaacggta tctacattat cgaccttcag
      361 aagagtgtaa agaagcttga agaagcttac aactttgttc gtgagctttc agcagaaggt
      421 aagagcgttc tctttgtagg tacaaagaag caggctcagg attctgttaa ggaagaggca
      481 gagcgtgcag gcgcttacta tgtaaacgca agatggctcg gcggtatgct cacaaacttt
      541 gcaacaatca gaagaagaat tgctcgttta aagcagctcc gtgctatgca ggaagacggc
      601 acatttgatc ttcttcctaa gaaggaagtt attaagctca acctcgaaat tgagaagctt
      661 gagaagttca tgggcggtat caaggatatg acagagatgc ccggcgcact tttcattgtt
      721 gatccaagaa aagaaagaat tgccgtttca gaggcaaaga agctcaatat cccgatcgtt
      781 gctatcgtag atacaaactg cgatcctgat gagattgact atgtaatccc gggtaacgat
      841 gacgctattc gtgcagtaaa gcttattgca ggtgcaatgg ctaacgctgt tatcgaaggc
      901 agagagggcc gcatgggcgc tgcagctgtt gaggaagaga cagctgaata atcatttgtg
      961 attataaccc cgttttatat aaatttattt gaaaggatgt tttcaaaatg gcagcattta
     1021 cagctcagga tgtaaagaca ttaagagaaa aaacaggctg cggcatgatg gattgcaaaa
     1081 aggcactcac aaatgccgac ggtgatatgg ataaagcagt tgacttttta agagaacagg
     1141 gacttgctaa gcaggctaag aaggcaggca gaatagcagc agaaggcgtt gcatacgctt
     1201 gcacaaacga tgacctctca gtaggtgttg ttgtagaagt taacgctgaa acagactttg
     1261 ttgctaagaa cgattcattt atggattttg ttaaggcttg tgctaacaca attatcgagc
     1321 agaaccctgc tgatgttgag gctctcctca cactcaaggc aacaggttca gagcaaacaa
     1381 tcgctgaaat gcttcaggaa aaggttctca caattggcga gaatatccag attcgtcgtt
     1441 ttgagcgtat ggaaggtgct tgtgttgctt atgtacacgc aggcggtaag attggcgttc
     1501 tcgttaactt tgatacagac ctcgctgcaa agccggagtt tgttgcatac ggcaaggatg
     1561 ttgcaatgca gattgcagct cttaacactc cttacctcaa tgagagtgaa gttcctgcag
     1621 aaatcatcga gcatgagaaa gaagttatgc gtcagcaggt aatcaacgaa ggtaagccgg
     1681 agcagattgc tgacaagatt gttatgggta agattggtaa gttctacaag gagaactgcc
     1741 ttcttgacca ggagttcgtt aaggacaata agatcaacat caagcagtac acacagaata
     1801 ctgctaagga gcttggcggt tctatcgaaa tcaagaaatt cgttcgcttt gaaaagggcg
     1861 agggcattga aaagcgtcag gatgactttg ccgcagaagt tgcaagcatg gttaaataat
     1921 tattttggcg tacaaagcat aataatcgga gcagagaatg acttctctgc tctcggtttg
     1981 cagaaaactc gaaattcagt ttgaccgaat ttcgagtttt aatttttaag aaattttaaa
     2041 tatcgcaaat tgcaatagca agttgaaaaa ataaatttta ttttttcgaa attaacgctt
     2101 gacaaacaaa tgtatatgta atataatagt caggtaaaat aaagcaaagc agagatgctt
     2161 tcacctgtgg ggtgccgtct gcacgggtca ataccgaagt tttctagtat tgttatgcgg
     2221 acggagtgtt tctgtccgca tttattgttt ttatgagact ttggaggtgt ttacctatca
     2281 gcaagaaaga aattcagatt aacgaagaaa tccgtgacaa agagttgaga atcatcggct
     2341 ctgatggtca gcagcttggc attatgtcag ctaaagaagc acttgatttg gcagaacaga
     2401 agaaccttga cttggttaaa attgctccgc agagcaatcc tcctgtatgc aaaattatgg
     2461 attacggaaa ataccgtttt gaacaggcta aaagggaaaa ggaagcaaga aaaaatcaga
     2521 gagttgttga tatcaaggaa gtaaggcttt ctcttaatat cgatacacac gattttaata
     2581 caaagcttaa taatgctctg aagtttatca aacacggcga taaggttaag gtttcaattc
     2641 gtttcagagg tcgtgaaatg ggacaccctg aaatcggtct tgaaacaatg aaacgatttg
     2701 ctgatgcttg cagtgaaaca gctgttgtcg aaaagcctgc aaagcttgaa ggcagaaata
     2761 tgcttatgtt tcttgcacca aagactaata agtaattaaa gctaaggagg atactaatat
     2821 gcctaaaatt aaaactcaca gcggtgccaa aaagagattc aaactttcta agaatggtaa
     2881 ggttatccgt gctcacgcta acaaaagtca catcttgaac aagaagacaa caaagcgtaa
     2941 aagaggtctt cgtcagactg ttgctgcaga taagacaaac acagctcaga tcaagcgtct
     3001 tattccttac aaataatcat tgaggactac taactaacat aaacggaggt aatatataat
     3061 ggcacgagta aaaggtgcga tgatgactcg caaaagaaga aaaaagacat taaagcttgc
     3121 taagggttac tggggtgcaa aatcaagaca ctttaaaatg gctaaacagg ctgttatgaa
     3181 gtccggcaat tatgcatata tcggtcgtaa gcagagaaag agagatttcc gtcgtctttg
     3241 gattactcgt atttcggctg cctgcaaggc taacggcaca aattattcaa ctttcatgaa
     3301 cggcttgaag aaggctggca ttgctcttaa ccgtaagatg ctctctgaaa ttgcaatttc
     3361 agaccctgct gcttttactt cacttgttga acaggctaag aacgcttaat ttaacttact
     3421 ttgtaattgc gtttgggtta tcccaaacgc aattgttttt tacagaaaat tggatggttt
     3481 tatgactata ctttcaagta aagataattc taatataaaa aatgccgtaa agctcaggaa
     3541 aagtgcaaaa tataggcgtg aatcaggact tttcattgca gaaggtgttc gcatttgtat
     3601 ggatgcaatg ctcagtaagg cacatattga cacctttttc gcaagtgaaa aggccagtga
     3661 gaaatatgcg gatgactttt caaatctgca aaaatattcc gacaaaacat tcattgtatc
     3721 tgataagata tttaatcaga tgtgtgatac agagtcgcct caaggatttc tgtgtgtaat
     3781 aaaagcactt gacaaaactg cttgttttga taaaataaag actggtgaca aatttttggc
     3841 actcgataat ctgcaggatc ccggcaattt aggtacaatt cttagaacgg ctgaggctct
     3901 gaatgttacc ggtatcgttc tttctgccga ttgttgcgat atttacagtc ctaaagttgt
     3961 aagaggcagt atgggggcag tgttcagact tccgtttgtt gtgtcggata caatttcagg
     4021 ttatctgaat gcacacagtg aacttgaatc ttatgcggcg gtcgtatcgg cgtcggctga
     4081 taagattaca acgactccct ttgtttcgcc ttgcatcgtt gcagtaggta atgaaggaaa
     4141 cggtcttaaa gaagaaacaa taagtgcctg taaacataaa attactattc ctatggaggg
     4201 aagagccgag tcgcttaatg ccgccgttgc ggcgtcaata ataatgtggg aaatgatgaa
     4261 atgatttcgc gtaatgcctg ttactggatt tggattacac tttcgatagg ctataataat
     4321 ccaaaggtaa agagaatatc cgaaatgtat tccgatgtgt ctgcctttta ttacggtggt
     4381 atttcggagt ggcgtctttg cggaattttt tcacaaaaag acattgaacg attttcttca
     4441 accggtcttg atgacgctaa gaaaatcgta gacagatgta ttgaatgcaa ttattcgata
     4501 ttatgcattg acgatgaatt gtttccgaaa tgtctttaca atattgaatg tcctcctgct
     4561 ctcatatatg taaacggtgt tatgcctgat attgataata ctttttcaat cggtattgtc
     4621 ggtactcgca gagcaactaa gtatggaata gaaaattcct atcgctttgc atatgcactt
     4681 tcaaaatacg gaacaattat agtcagcgga ggcgcattag gtgttgacgg tgcgtcgcac
     4741 agaggcgcac ttgctacgga cggaattaca atttgtgtca gaggttgcgg acttaattgt
     4801 tcatatctca gggaaaattc agacattaga agtacaattc caaaaagagg tgctgtcatt
     4861 acagagtatc cgcctgatga aacaccgagg aattactatt ttcctgcccg aaacaggatt
     4921 atagccgctt tatcggacgg attgcttgtt atggaagcgg gaaagaagag cggttcgctt
     4981 attaccgcaa atcttgctgc agaacaaggc aaaacaattt ttgctctgct cggaaataac
     5041 agccctcaga atgaaggttc aaacgctctt attaaagaag gtcttgcaat acccgttacc
     5101 gattttatgg acatactctg cgagtttgac aacctgtatg caaccactga tgatgaattt
     5161 gatattgaca atatttctct tgctgatacg ggcaattttc ctgttaaagg tataagaaaa
     5221 caagctcctg caagaattta tgttaataaa caaaatgatc aacagccggc taaaaccgca
     5281 gtgccgtatg tatctgaaaa atcagaaacg gttgtgcaaa aacctgttca taaagaaaac
     5341 ttaaatttgc caaaaacagc tcaggatgta tatgaataca tcggtaatga gcctgttcat
     5401 atagataaaa tttcagctga tttgaaaatc cctgtgttta aggtgcttac cgcacttacg
     5461 atgcttgaaa tgaaagatct tgtttcggca ctacagggaa gaaattatat tttaaaatga
     5521 tatttaccga atattcttat tcgttatccg atattccgtt gcccttttac acataacagg
     5581 gcagaaaggc agtgaaaaca tatgtcagat ttagtaattg ttgagtcacc tgctaaggca
     5641 aaaacaatta aaaaatacct tggtagcggt tacgaagttg ttgcctcaat gggtcatgtc
     5701 cgtgatttgc ctaaatcaag attaagtgtt gatataaaaa agaactttgc tccaaagtat
     5761 gaaattataa aaggaaaaga aaaacttgtt gatgatttaa aatcagccgc tgaaaaaagc
     5821 gagcatattt atttagcaac cgaccctgac cgtgagggag aggcaatttc ttggcatctt
     5881 gcctatattc tcggtttacc cacagatgaa actaaccgtg tggaattcaa tgaaattaca
     5941 aaatccggtg ttacaaacgg tatgagcaat ccgagatcaa ttaatattga ccttgtaaat
     6001 gctcagcagg caagaagaat tcttgacaga cttgtcggtt ataaactcag tccgtttatt
     6061 tctcagaaaa tcagaagagg tctttctgca ggacgagttc agtctgttgc tgtaaggatt
     6121 attgttgaca gggaagagga aatcagaaaa ttcaagcctg aggaatactg gtcgattgat
     6181 gcaaagttca tcccaaaggg cagcagaaaa tctttttctg catctttata ttccgatgaa
     6241 aacggtaaga ttaagattac caacggtgaa caggctgata aaattgaggc tgatcttcag
     6301 aatgccgagt atgtaattac aaaggttaag aacggtacaa gaaagaaatc tcctgcacct
     6361 ccgtttataa catcaactct tcagcaggaa gcatcgagaa aactcggttt ccagtcgaga
     6421 agaacaatga aggttgctca ggaactctac gaaggcgttg atatcgaagg tatgggtgca
     6481 accggtctta tcacttatat gagaacagac tcactcagaa tttcaaatgt tgcaattgaa
     6541 gaggtaaccg aatacataaa aaattcttac ggtgaaaagt ttcttccgcc aaagccaaga
     6601 ttttttaaat caagaagtaa tgcacaggac ggtcatgaag ctataagacc gtcaatgccc
     6661 tctcttactc ccgacagcgt aaaggcaagc cttacatcgg atcagtataa gctatataag
     6721 ctcatttgga acagatttac tgcatcacaa atggctgatt gtatccagaa aacaacacag
     6781 gctgatattg ttgccaacgg ttatacattt aaggcgtcgg gatacagagt cgattttgaa
     6841 ggctttacaa cactctatgt ggaaagcaag gatgttgaag aagaaaagac tacacagctc
     6901 ccaccacttg aaaaagatat gattgtgaga tgtaaagagt tgaagaaaaa tcagcacttt
     6961 acacagccgc ctgcaagata tacggaggcg tcactcatca aagcgcttga agaatacggc
     7021 atcggcagac cgtcaactta tgccgcaaca attacaacaa ttacaagtca tgaatatgtt
     7081 aagcgtgagg gcaaaagcct tgttcctacg gaactcggtg aggttacaac caatcttatg
     7141 aaagagcgtt ttccaaagat cgtaaatgta aagtttacaa atcagatgga ggaagacctt
     7201 gataaggttg aaaaaggaac agaagagtgg catgctttgc ttgacaattt ctacggagat
     7261 tttgataaaa ctctcaaaaa agcaaaagca gatatggaag gcgtaaagct tcacttaaaa
     7321 gaagacgaaa ccgatattat ctgtgacaag tgcggtcgtc agatggttgt taaagtcggc
     7381 agatacggta aatttattgc ctgccccgga taccctgaat gtaaaaacat cattaagtat
     7441 gtagaaaaaa cgggtgttaa gtgtccgaag tgcggcggcg atgtcattat caagcgtacc
     7501 aagacaaaaa gaatttttta cggctgctcc aactatcccg agtgtgattt tgtaagttgg
     7561 aatgagccaa caaacgagaa gtgtccgcag tgcggaggaa ttctcttcaa gaaaaaaggc
     7621 aaaaagccga cattgttctg tgcaacagag ggatgcggtt atactaaaac agcagatacg
     7681 gatgataaat aaatgcatat taatgtaatc ggtgcaggac ttgcaggttg tgaggcggca
     7741 atgcagattg cctctcacgg aataaaagtg catctttatg aaatgaaacc gaataaaaaa
     7801 acgcctgctc atcatactga gaaatacgct gaacttgttt gttcaaattc tcttaaagcc
     7861 atgcgtgttg aaagcgccgc agggcttttg aaagaagaaa tgcgcagact cgattctttc
     7921 cttatgaagt gtgcagatgc ctgcaaagtt cctgcaggcg gagcgcttgc cgttgacagg
     7981 gatatttttt cttctcttgc aacggaaggg ataaagagct gtgagcttat tgaggtttac
     8041 gaagaagaag tttgtgaaat tccgaaagat gaaatcacgg ttgtggcaag cggaccgctt
     8101 acttctgaac cgcttgccga atatataagg ggaatgttcg gttcctcact ttcgtttttt
     8161 gatgccgccg ctcctattgt taccgctgaa agtattgaca tggagtacgc tttttgtgca
     8221 tcaagatacg ataagggcga cggtgacgat tatatcaact gtccgatgaa caaggaggaa
     8281 tatgaaacct tctacaatgc actcatatcg gcagaaagag cgcctttgca tgattgcgat
     8341 gtcagcaatc cgaaggtgta tgagggctgt atgcctgttg aggtaatggc tcagaggggc
     8401 gagggaacac ttcgctttgg acctatgaaa cctgtcggtc ttgtgaatcc aaagacaggt
     8461 cacagacctt gggcggttct tcagctcaga aaagaaaaca aagccggcaa tatgtacaat
     8521 cttgtaggat ttcagactaa tcttaaattt cccgaacaaa agagagtgtt ttcgcttatt
     8581 cctgcacttc ataatgcgga ctttgttcgt tatggtgtta tgcacagaaa tacttttctt
     8641 gattcgccga gaatacttaa cagcgatttt tctgttaagg acaatcatag tctttttttt
     8701 gccggtcaga ttacaggtgt tgaaggatac atggaatccg cagcttcggg aattatggca
     8761 ggaataaatg cttgtcgagt tgctgagggg aaaagcacaa ttacattacc aaatgataca
     8821 atgattggcg cactttcggc atacatctct gattcgtcgg ttaaaaagtt tcagccaatg
     8881 ggtgcaaact tcggagtttt gccggagctt gaaaaccgcc cgagggataa aaaagagcgt
     8941 ggtgcagttt attctgcaag agcgttgaaa agtttggata attatttaaa ggatatttaa
     9001 ggtgaatgga atgaacataa ttgttgacgc attcggcggt gataacgctc cattggaaat
     9061 tatcaaaggc tgtgttgagg cagttgccga atataacata aatgtaactt taaccggtga
     9121 tgaaaccaaa attaaaaatg tattttcgga aaactcactt tcaatgaata atattgatat
     9181 ttgtcattgt acacagaaaa tcacaatgga agattctgct gagtccgttc ttaaagaaaa
     9241 aaaggacagt tctatggctg taggtcttcg cttgcttaac gagggcaagg gcgatgcatt
     9301 tgtcagtgca ggaaacagcg gtgcattgtg tgtcggtgca actcttgcaa taaaaagaat
     9361 caaggggatt aaacgccctg catttgctcc tgttatgccg tctgaaagcg gattctttat
     9421 gcttatggac ggtggtgcaa atgttgagtg tcgtcccgaa atgctgtatc agtttgctgt
     9481 tatgggttca atatatatgg aaaaggttat gggtatcaaa aacccccgtg tcggacttgc
     9541 caatgtcggt gcagaagaac acaagggaac agaactttac agaaatacgc atgaacttct
     9601 tcagaacagc aacttgaatt tcatcggaaa tgtagaaggc agggatattc cgaacggagt
     9661 atgtgatgtc gtagtttgtg acggctttac aggtaatttg attctcaaaa catatgaggg
     9721 cgttgctctt gtaatgatga atcagattaa aaatatgttt atgggcagcg tcaagggtaa
     9781 acttgcagca ggtcttgtta tgaaggacct aaaaaatatg aaaacgcatt ttgattacaa
     9841 cagatacggc ggtgcgccta ttctcggtgc atcaaagccg gtgttcaaag cacacggaag
     9901 tgccaaggct gttacagtta agaatgctat cagactttct gttgaatatg taaaagcaaa
     9961 cgctattgaa gcgatctctt catcacttta atttttgcaa ataatgttgc tttaggagtt
    10021 ttataatgga agatttacaa aagagaatcg gctatacatt caaaaataca gatttgctta
    10081 cagaggcact tactcattct tcttatgcaa atgaacataa ggcgaaacac ataaaatata
    10141 atgaaagact tgaatttctc ggcgatgcgg tactttcaat tgttgtttcc gattatattt
    10201 tcaagcattg tcccgaactt cccgaaggcg aattgacaaa gctcagagcg tcacttgtct
    10261 gcgagaaaac tctttttgaa tttgcaaagc agattgaact cggaaagtac cttgtgttaa
    10321 gtaaaggcga aagaaacaac ggcggtgcag aacgcccgtc aattctttcg gatgcctttg
    10381 aggctttgat tgcagcaatt tatattgacg gtggtattga tcctgcgtcg aaacatattc
    10441 ttaattttgt aattcctgca atcaagaact caaagaagaa gaagcttaac gattataaaa
    10501 ccgccttgca ggagattatc cagaagaatc ccggtgaaca gcttgaatat gttctcgtgg
    10561 gtgaaagcgg tcctgaccac aacaaacact ttgttgttga agttcatctc aacagtaatg
    10621 taatcggcaa aggcggcgga agaagtaaga aagaagcgga acaacaggcc gccagagaag
    10681 ctttggagct gatgggttat taaacacagt aatgtatcaa tttttattcc gcacaacggt
    10741 tgtccgcata tgtgcagttt ttgcaatcaa aataagataa caggtcaggc atatcagccg
    10801 actccccttg atgttgaaca gacacttggt aaagcggctt ctgatttggg cgaacgcacc
    10861 aaaaatgctg agattgcatt ttttggcgga agttttacag ctattgacag aacctatatg
    10921 ttatctctcc ttgatgcaac taaaaagttc agagataaat tttgcggaat aagaatttct
    10981 acccgtcccg actatattga taaagaaatt cttgatattt tgaaaagcta tggtgtgaca
    11041 tcaattgaac ttggtgcgca atctatgtca gacgatgtac tgcttgcaaa tgacagagga
    11101 catagcgctg aaagtgtttt caaatcctca gagcttatta aaagttatgg cttttcactt
    11161 ggcttgcaga tgatgacggg actgtataaa tcagatattc aaaaggattt atacacagcc
    11221 gaaacatttg tgaaaattaa tcccgatacc gtaagaatct atcctacggt catcatgaaa
    11281 gatacaaagc ttgccgaact ttaccttaac ggagaataca caccgtatag tcttgaaaag
    11341 tccgtcaacc tttgttcaaa gctgattgag ctttttgaaa agaacaatat aaaaattatc
    11401 aggctcggac ttcattattc cgacagcctt gtaaataaca gctacggtga caactatcac
    11461 ccggctttta aggaattgtg cgaaaataag cttttttacg ataagtttat cgaaaaagca
    11521 aaagattttc ccgatttgaa tgaaattaaa acggttgtta tcaattctaa atctctgtcg
    11581 aaatttctcg gacaaaagag aagtaatgta aaaaaattga atgaactcgg atataatttt
    11641 tcggtttgtt ttgacgattc tttgcagaaa tatgaattat atttaaaata ggagcttgct
    11701 gatgcgatta aagtctttgg aaattcaagg tttcaagacc tttcccgata aaactaaatt
    11761 atcttttgaa cagggaatta cctcggttgt cggtcctaac ggttcaggca aaagtaacat
    11821 cagtgacgct atccgctggg ttctcggtga acagtccccg aagagccttc gttgtgctaa
    11881 aatggaagat gttgtcttta acggaacaga taagcgtaaa agacagggct atgccgaagt
    11941 tactctgaat attgacaaca aagaccgtgc tcttgatttt aacggtgatg acatagccgt
    12001 tacaagaaga tactacagaa gcggcgaaag cgaatatctt atcaataaag cggctgttcg
    12061 tcttaaagac atcaacgaac tctttatgga cacaggcctc ggtcgtgacg gttattcaat
    12121 gatcggtcag ggtaagattg acagcattgt ttcttcaaag ggtgaggaca gacgagagat
    12181 ttttgaagaa gctgcgggta tttctcgcta cagatacaga aaaattgacg ctgagagaaa
    12241 gctaaaaagt acggaggaca accttttaag acttcgtgat attgtgaccg aacttgaaga
    12301 aagagtcggt cctcttgaaa agcagtctga aaaagctcaa aaatttcttg aatattccga
    12361 agaaaaaaga ggtcttgaaa ttgcactctg gcttttaact cttgaccgtt cacaaaatga
    12421 attaaaggtt caggatgaga aaatttcaat tgcaaaatct caatacgatg acgcagaaac
    12481 cgcacttgaa caaattcaga ccgaaagtga agaaatttac ataagaaacg gtgagtattc
    12541 ttctaaaata gaaaatatca gaaccgaaat tgcagatata gaatcacaga tttccgaaaa
    12601 gcgttcttta gtgtctgttg ccgagaacga tattctgcat aacaatgaaa atattgacag
    12661 aataaaaaat gatattgcac agtttgatgt caatgcggat gaacttgtaa aaagaattgc
    12721 cgataaggaa aaagaaattt cggaacttga cgctgatata atcgctaaaa agaaaaggta
    12781 cagtgagctt tcggaacagc ttaacgaaat taatacgact tcaggtaaaa gcagtgatgt
    12841 tttgcaggaa attactgccg agctttctgt tcttacttca aagtcggctg atgcaagagt
    12901 taccgagatg actgccgaaa gcacggtttc ggaacttaat tcaagaatag aatctttaat
    12961 ttcatctaat gacgaaaaga aaacgcagtc tgaagaactt gccgatattt caaaagatta
    13021 tgataataaa atcggtgttg cagaagaaaa aattgaatcg ctgaaaaata caatagacgg
    13081 tttacagctt aaactgtctt caaaagaaaa gaaaaaagac gaactccgtg aagaaagcaa
    13141 caaacttatg cttgatgtca aggagcatat gataaaaatt tcattccttg aaaatcttga
    13201 gcgtaatctt gagggctttt caaagagcgt aaaaacagtt gtcaatgctc aaagtcacgg
    13261 caaggttcac ggaatatgcg gtcctgtttc acgagtaatt tcagttccga aagaatacgg
    13321 agttgcaatt gaaacggctc tcggttcggc tatgcagaac attgtaaccg aaaccgatga
    13381 ggatgcaaag cgggcaatta gatatcttaa atccgttgac ggcggtcgtg caacatttct
    13441 tccgcttaac acaatcaaac cgagagaact ccgtgaaaac ggtcttgaag attgctacgg
    13501 atttgtaggg gttgccgcaa atctttgttc atgcgataat aaatacaacg gaatcctcta
    13561 ttccttactc ggcaaaattg tgattgcaga ggatttgaac tgtgcaacaa caattgctaa
    13621 gaaatattct tacagattta aaattgtaac tcttgacggt caggttgtta atgccggcgg
    13681 ttcactcaca ggcggttcgc ttaacagaaa cacaggactt ctcagcagag caaatgagat
    13741 tgaaacactt aaaaagcaga ctaccgtcct tgaacagaag tcaaaagatg ccgaaaacag
    13801 aagtattgaa atttctcgtg aatattcggc gtttgaggct cagcttctcg gttcaagagc
    13861 agatctttca aatcttcgac aagaccttgt aagactcgag gctgaaaaga gagcttgtga
    13921 caatgaactt gccaatgcag gtgcttctgt tgaaaattca atgaatgaaa ttacaaattg
    13981 tcgtcggaga attgaaactc ttgaaaaaga taaaaattcc gcaaagcagc tccttgttga
    14041 actcaataag aaaatccttg ctgccgaaga aaaggtaaat gaacttacgg gcagcagagc
    14101 agagcttaca aaaaagcgtg aagaaatttc tttgcagtta cagaatctgc gacttgaaat
    14161 tgtttcttcg caaaaggata tcgacgctct taattcggat attgtttttg caaaatcaac
    14221 aggcaatgaa aaccttgaaa gaaaagcaga attaaatcaa gagattgaga gaatcaaaag
    14281 tgttattctt gaaaagacag agcttataaa ttctacaaat gcagaaattg aaaagttgat
    14341 tgccaatcag aatgaacgct caaaagctat tgaaaaaatc aatgctgaca gagaacagct
    14401 tgaaaaacgc agtgctgaaa tcagaacggt tgagcgtgat aaaacatcag agcgtgaaac
    14461 ctcaggcaga gagcttgcca gacttgaaga gagaaaaatc aacattcaga agcagtatga
    14521 tgatattatc tcaaagcttt gggaagaata cgaactgaca agacgagagg cagaagattg
    14581 tgctgttgaa attgatgact caaaacaggc gcagaggcga cttaacgagc ttaaacaaaa
    14641 aatcaggtct ttgggaaatg taaatgtatc cgctattgaa gagtacaaag aagtttccga
    14701 aagatatgaa tttatgagcg cacaggtaaa tgatgttgaa aagtcaaaga aagagattga
    14761 aaagcttatt actgacctca caaagcagat gaaggaagtg tttgtcgaga gctttgatca
    14821 gattaataag aactttacct acaccttcaa agaacttttc ggcggcggta cagcgtcact
    14881 ttcgcttgcg gatccggaaa atattctgac aagcggaatt gatattcttg ttcatccgcc
    14941 gggtaagatt gttataaatc ttgaggcgtt gtcaggcggt gaaaaagcgc ttgttgcaat
    15001 tgcactttat tttgctataa tgaaagtaag acctgctccg ttctgcgtaa tggacgagat
    15061 agaggcggct cttgatgatg taaatgttta tcgttttgcc gcatatcttc gcagaatgac
    15121 agataataca cagtttattc tcattacgca tcgccgtggt acaatggaag aagccgatgt
    15181 gctttacggt gttacaatgc aggatgaggg catttcaaaa ttactcgaac ttcgttcaac
    15241 cgaagttgcg gaaaaactcg gtatagattc aaagtaatta taaggacagt gataatatgg
    15301 gatttttcaa aaagattaaa gagggactga aaaaaactcg tgatgcggtt gtaggtcaga
    15361 ttgactcaat gctcaaatct tttacaaaaa ttgacgatga gctttttgaa gaacttgaag
    15421 aattgctcgt tatgggc
//
LOCUS       DAOMVE010000029        14948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_842_length_14948_cov_3.953267, whole genome shotgun sequence.
ACCESSION   DAOMVE010000029 DAOMVE010000000
VERSION     DAOMVE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14948
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_842_length_14948_cov_3.953267"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(703..1113)
                     /locus_tag="PLS64_02870"
     CDS             complement(703..1113)
                     /locus_tag="PLS64_02870"
                     /inference="COORDINATES: protein motif:HMM:NF018621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HRL42808.1"
                     /translation="MTKSLSFKFRPHHSLCVQFFEGKGYSDEFVCGMAKIVSVLENNN
                     ANLTLTGGCDEICSSCPNRDNGSCDVTGKASCYDKRCLDLLGMKIGDTADWKELSRIA
                     RENIILKDRLSQVCGDCKWSHICRDKADKMKSLH"
     gene            complement(1091..1639)
                     /locus_tag="PLS64_02875"
     CDS             complement(1091..1639)
                     /locus_tag="PLS64_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HRL42809.1"
                     /translation="MTEAKTNSKSKSNVLSLVYMAMFVAIITVCAQIQIPMTVPFTLQ
                     TLGIFMAAAMLGWKRGLISVAVYVLLGAIGVPVFAGFSGGIGVLGGPTGGYIIGFLFT
                     ALIVGLMTDFLGRKLWVLAVSMVAGLAVCYLFGTIWFMIAMHTDFVAALLTCVVPFLL
                     ADAAKIVVATVLVNRLDKIVKL"
     gene            complement(1910..2776)
                     /gene="fba"
                     /locus_tag="PLS64_02880"
     CDS             complement(1910..2776)
                     /gene="fba"
                     /locus_tag="PLS64_02880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HRL42810.1"
                     /translation="MPLVNAKEMLTKAKAGHYAVGQFNINNLEWTKSILLTAQELNSP
                     VILGVSEGAGKYMCGYKTVVGMVNGMLEELNITVPVALHLDHGSYEGAKKCIEAGFSS
                     IMFDGSHYPIEENIEKTKELVATCNKLGLSLEAEVGAIGGEEDGVIGMGECADPKECK
                     MVADLGVTMLAAGIGNIHGKYPANWKGLSFETLDAIQQLTGEMPLVLHGGTGIPADMI
                     KKAISLGVSKINVNTECQLAFAAATRKYIEEGKDLEGKGFDPRKLLAPGAEAIKATVK
                     EKMELFGSVNKA"
     gene            3309..5048
                     /locus_tag="PLS64_02885"
     CDS             3309..5048
                     /locus_tag="PLS64_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRL42811.1"
                     /translation="MDNFDNNNMGGFEPQKPFTEKPVQNNNMNTEQTIPVSEPETQEE
                     QAQNVQPQNGNTPYGDYIFGNNNQNTANNANPTENVPPYQQNQNPNPNTYAQPWQYGY
                     GQYPYSGQNPQYNQNNQQYGQYQNNTGYNQNVPPYQQNMYGQPPYGNNGTYNQQYNPQ
                     MFAQYPQKKTKGGIIALIIVLCSLLTIGFIGMMVYGFSADIKEDLNNDRSDSGNSFRL
                     PNKDSTTPFETLPDTSSQGKTHDESDYSDKVNKDYSGMKLESNPKDAKTNNSYTAAKA
                     SEKVSDSVVGILCYSDDVPDQADTTTASSQGSGIIFSQDGYVITNAHVIGNSKTAYAI
                     RVVTSDGKVYKAGVVGYDSRTDIAVLKMDDAKGLTPATFGDSSQLEVGQDIIVVGNPG
                     GLDYQNTTTKGVISALDRKLSTSSLTKYIQTDAAINPGNSGGPLVNYYGQVVGITTSK
                     IVSETYEGMGFAIPSQTVKSIVDTLVKNGYVEGRVKIGISGIAVTSDQASNYNIPQGI
                     YVQSIVSGGPCDGTSLKEGDIITEVDGETITSFADVYAILETHKPGDKIKVKYYSSSS
                     GDGEVEITLQEDK"
     gene            complement(5253..6050)
                     /locus_tag="PLS64_02890"
     CDS             complement(5253..6050)
                     /locus_tag="PLS64_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42812.1"
                     /translation="MKKRLKGFFQRLARWLNVREMRQHKVKAGVYIIIRLILVSLLTA
                     GILNGKWENVMTCVLTLGLLMLPLFIDRKLSVALPSVLETIVVLFVFAANVMGELGAF
                     YEKIPIWDSLLHTVNGFICAGVGFGLTDILNRSERVKLSLSPMFVCLFSFCFSMTVGV
                     VWEFFEFGADMLFEKDMQKDTVITAIHSGLISGKPNVIMHIRDITSTVVNGENLGING
                     YLDIGLIDTMKDLLVNFVGAVVFDTIGWFYLKGRSAGFLRNFIPVKK"
     gene            complement(6194..7519)
                     /locus_tag="PLS64_02895"
     CDS             complement(6194..7519)
                     /locus_tag="PLS64_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HRL42813.1"
                     /translation="MAELKYDKKTGRLLFTKQMKKEYTILMPNMAPIHFKILEKVFNY
                     YGYKSDLLDTTGPEIAQTGLKYVHNDTCYPALLVIGQFINALQSGKYDVHKVALMITQ
                     TGGGCRASNYIFLLRKALKKAGFEFVPVISLSFGMEHNSGFSLTLPLIRRFVGGLVYG
                     DQLMLLSNQTKPYEVNKGESMALVDEWTDKISDMLIQGKGYTLEEIDETLDKITKSFT
                     KIELNKVPKVKVGIVGEIYVKYSKMANNGLEDFLADQDCEVCVPGLMGFASFKVDNRI
                     EDYKMFGGSRVKYKFCSMLLNYLTKLEGLMVDAAEKYGFVPPHRYAHTKELVNGVIGY
                     GSKMGEGWLLTAEMIELADTGYENIVCTQPFGCLPNHINGKGAIRKIKEIRPNANIVT
                     IDYDPGAPKVNQENRIKLMLAVGKEELKKKLEAEKNGEKAESSEKSEKK"
     gene            complement(7537..10440)
                     /locus_tag="PLS64_02900"
     CDS             complement(7537..10440)
                     /locus_tag="PLS64_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HRL42814.1"
                     /translation="MKLGLDIGSTTIKCVVLDDENKLIYSTYERHYSRITEKIAEILA
                     LVRTRIDGVENASVALSGSAGMGVAESVGVDFIQEVYATRVAANTFIPGTDVIIELGG
                     EDAKILFLTNGLEVRMNGTCAGGTGAFIDQMATLLNVKLEDMDELAKQHEKTYTIASR
                     CGVFAKTDIQPLLNQGARKSDIAESIFNAVVSQTVAGLAQGREIEGQIVYLGGPLTFM
                     SELRNCFDRTLGTKGICPENSLYYVACGAALCAEKTIDFDEVIEKVKNYRGSGNFAFN
                     QPLFKNEEEYKEFCDRHAKADVKQKELKGYTGKAYIGMDAGSTTVKGVVLNDDGELLY
                     SKYLPSKGNPVEIMKQFLDEVYEINPEINVVSSAVTGYGEDIVKNAFAVDYGIVETIA
                     HFTAAKYFMPDVEFIIDIGGQDIKCFKIHNGAIDNIFLNEACSSGCGSFLQTFANALG
                     YEIADFAKLGLFAKRPVDLGSRCTVFMNSSVKQAQKDGATIEDISAGLSLSVVKNALY
                     KVIRASSPDELGKRVVVQGGTFLNDAVLRAFEQEMGVEVVRPNIAGLMGAYGAALYAK
                     KKSKGVGKSTITDKKGLDEFVHEIKVANCGMCNNNCRLTINSFGKGRKFIAGNRCERP
                     ITKKAPANDMNMYAYKLNLIDSYKPVEGIRGKLGIPMALNMYELYPFWYRFFTELKFE
                     VFHSPYSTRKLYQRGQQTIPSDTVCFPAKLVHGHIQTLIDMGAETIFYPCLSYNFDEH
                     LGDNHYNCPVVAYYPEVIKNNMKDIRKVHFIKEYFGIHRPNDFPKKAYERLSFHFPDL
                     TLNEVRNAAKLAYQEQENYRKKVIDKGNEIIAKAEKEGKKIFVLAGRPYHIDPEINHG
                     IDRLISGFDVAIVSEDVISPRVEHFRTHVLNQWTYHARLYAAAKYVTTRKDMELVQLV
                     SFGCGVDAITTDEVREILESKNKIYTQIKIDEITNLGAVKIRIRSLLAALEND"
     gene            complement(10726..11331)
                     /locus_tag="PLS64_02905"
     CDS             complement(10726..11331)
                     /locus_tag="PLS64_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42815.1"
                     /translation="MTILDSVKGTVNTAVDTISSVAQTIVEKNRTNAKLNRLRLVMKS
                     ESELMNRAYIALGKIMYDSRKKGTEINDAECEKLLKVIDTSKAKIAKARECYRKIVNS
                     SNDIFYGNPEVAPEVKEDDIVDITVACSNEGDYKSSPFKEEKPVEKQPEAVTEEKAEP
                     EHLTNICDLKAELEAKREEGFSESADSDEADDGEAPHGELF"
     gene            11524..12222
                     /locus_tag="PLS64_02910"
     CDS             11524..12222
                     /locus_tag="PLS64_02910"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HRL42816.1"
                     /translation="MPEKKITKAIVCPMCGEMSKADVYTSINPSVTKGVRRRALDGEL
                     FAWKCPSCGYSARLTYPILYNDMKNRFMVYFIPKIDHFQLCDNELEEKYSNLRNISKR
                     IVPTFNSFKEKIFIFESGLDDMSVELTKLAISQTVSKKLNGAEIHDGYLSMYDRERNT
                     MGFTYFTGEKHTPYVQTARLEIYVKSKKIVDSLAYKDKKLKGFLKIDREWAENILYRY
                     KRMKHIEKLNKLKE"
     gene            complement(12507..13511)
                     /locus_tag="PLS64_02915"
     CDS             complement(12507..13511)
                     /locus_tag="PLS64_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HRL42817.1"
                     /translation="MAVTFEGMERRMPKIEKCLAEYGIANLEEARKICNDLGFDPYDI
                     VKGVQPIAFENAGWAYTLGCAVAVKKGAKTASEAAEAIGIGLEAFCIPGSVAEQRNVG
                     RGHGNLGAMLLRDETKCFAFLAGHESFAAAEGAIGIARNANKARKEPLRVILNGLGKD
                     AAYIISRINGFTYVKTQFDYFTSELKIVEERAFSDGERAAVRCYGADDVREGVAIMQH
                     ENVGVSITGNSTNPTRFQHLVAGTYKKWANENGVKYFSVASGGGTGRTLHPDNMGAGP
                     ASYGLTDSMGRMHGDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVACAVAVYEAVNK
                     "
     gene            complement(13533..14225)
                     /locus_tag="PLS64_02920"
     CDS             complement(13533..14225)
                     /locus_tag="PLS64_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42818.1"
                     /translation="MIYSKEVENMCTVAKGPKHGPAPIPEEGKWVKSYDIKDISGLSH
                     GIGWCAPQQGACKLTLNVKDGIVEEALVETIGCSGMTHSAAMAAEILPNKTLLECLNT
                     DLVCDAINTAMKNIFEQLVYGRSQTAFSEGGLPIGAGMEDLGKGLRSQVGTMYSTNAK
                     GVRYMEMAEGYVLKTALDENDEVIGYQFVKLGKMLEDIRHGVEPNEAYKNNIGQYGRF
                     DGAAKYIDPREE"
     gene            complement(14439..14633)
                     /locus_tag="PLS64_02925"
     CDS             complement(14439..14633)
                     /locus_tag="PLS64_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL42819.1"
                     /translation="MKNLRLKSARAAKDLSQQQLAELVKVSRQTISAIEKGDYNPTIN
                     LCIAICKALDKTLDELFWEE"
     gene            complement(14630..>14948)
                     /locus_tag="PLS64_02930"
     CDS             complement(14630..>14948)
                     /locus_tag="PLS64_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42820.1"
                     /translation="VLEINWAELSVQMFIGLFLSTAVFVGISILNDAYFTSNKGRRSL
                     LGSLAFVAGAECLGIAFGNKTFVTNGIANLDIIPIIVMIWTISIFVIVVIKKIIDKKA
                     VEE"
BASE COUNT         4301 a         3425 c         2932 g         4290 t
ORIGIN      
        1 atacttaaag ttatacagtt taaataataa tccaatttcc tccaaaagtc aatattttca
       61 ttcgtgaaga tagtttttta agtcaaatac tttaatgccc gagcgttatt ttctatatta
      121 aggtaacttt gtagccgagt gcctcggcag tcaattcgtg aagacagttt gtacaataga
      181 aaactaaaaa tgcccgagcg tgactttcta tattaaggta gttttttcaa ccaagtgcct
      241 ctacagccaa ttcgtgaaaa tagtttgtac aatagaaaac taaaaatgcc cgagcgtgaa
      301 attgttattg tctgtaccaa cctgtagggg cgttcatcgg acgcccacag gtaacggcag
      361 aattttcata acagattaaa ttttataaat gccgcaaaac aattgttata ttgtaggggc
      421 gaaccgtgtg cattgcacac ggtttgcacg caatgcgtgc aaattcgata cacaacattt
      481 atcacatcaa aacatatgtg actaaacaaa atgtttcatc aaggcgggcg tccaatgaac
      541 gcccctacaa cacaaattgc aaaaacaatt acaaatattt gctcgggcaa aatatgtttt
      601 aaaatataca aactgtctgc acaaacaggg aacggcggct cgccgtaaac aaaagacctc
      661 acgaaattcg tgaggtcttt tatcatgata tatctatgag gattaatgaa gacttttcat
      721 tttatcggct ttatcacggc atatatgcga ccatttgcag tcgccgcaca cctgtgaaag
      781 cctgtctttt aaaattatat tttcccttgc aattctcgaa agctctttcc aatcggcagt
      841 atcgccgatt ttcattccga gtaaatcaag acatcttttg tcatagcatg acgccttgcc
      901 cgtaacatcg cagctgccgt tgtcacggtt cggacacgat gaacaaattt cgtcacaacc
      961 gcccgtaagt gtaaggtttg cgttgttatt ttcaagaacc gacactattt ttgccattcc
     1021 gcagacaaat tcatcgctgt aacccttgcc ttcaaaaaac tgcacacaaa ggctgtgatg
     1081 cggtctgaac ttaaagctta acgattttgt caagacgatt caccaaaact gttgcaacaa
     1141 caatctttgc agcatctgca agaaggaacg gaacaacgca ggtgagaagt gcggcaacaa
     1201 agtctgtatg cattgcaatc ataaaccaga ttgtaccgaa aagatagcat actgcaaggc
     1261 ctgcaaccat tgaaaccgca agcacccaaa gctttctgcc gagaaaatca gtcataagtc
     1321 caacgataag tgccgtaaag aggaaaccga tgatataacc gcctgtcgga ccgccgagaa
     1381 cgccgattcc gcctgagaat cctgcaaaca cgggaacacc gattgcaccg aggagaacat
     1441 aaaccgcaac gctgatgagt cctcttttcc aaccgagcat agccgcagcc ataaagatgc
     1501 cgagagtctg aagtgtgaac ggaactgtca tcggaatctg aatctgtgcg cacactgtga
     1561 tgattgcgac gaacatcgcc atatacacaa ggcttaatac attacttttt gatttactgt
     1621 ttgtttttgc ttctgtcata aaattacctc tcctttgcaa aaccgcttct tttgttttgc
     1681 agattgtcaa ccaaattatt ttatcggttg acaattatta tagtaccgat tatcgcattt
     1741 gtcaacccga tttatgtttt aaagtgacaa ttacaatccg ttttgatgta acaattgact
     1801 ttacaaccgt cttataatac aactgagtta accaaacaga acataaaaac agggacagca
     1861 ttttgctgtc cctgaaattt ttgtaaagat taagtttgcg tgcttacaat taagccttgt
     1921 taaccgaacc gaagagttcc atcttttcct taacagtagc cttgatagcc tctgcaccgg
     1981 gagcgaggag ctttcttgga tcgaagccct tgccttcgag atccttgccc tcttcaatgt
     2041 actttcttgt agctgctgca aatgcgagct ggcactctgt gttaacattg atctttgaaa
     2101 cgccgagtga gatagccttc ttgatcatgt cggcagggat acctgtaccg ccgtggagaa
     2161 cgagcggcat ctcgcctgta agctgctgga tagcgtcaag agtttcaaag ctgaggccct
     2221 tccagtttgc aggatactta ccgtgaatgt taccgatacc tgctgcgagc attgttacgc
     2281 cgaggtctgc aaccattttg cattccttag gatctgcgca ttcgcccatg ccgattacgc
     2341 cgtcttcctc gccgccgatt gcaccaacct cagcctcaag tgaaagaccg agcttgttac
     2401 aagttgcaac gagttccttt gttttttcaa tgttttcttc gatcggatag tgtgaaccgt
     2461 cgaacatgat tgagctgaaa ccggcttcga tgcacttctt agcgccttca tatgaaccgt
     2521 ggtcaaggtg gagagcaaca ggaactgtga tgttgagttc ctcgagcata ccgtttacca
     2581 taccaacgac tgttttgtag ccgcacatat atttaccggc accctcagat acaccgagga
     2641 taacaggtga gttgagttcc tgtgctgtta aaagaattga ttttgtccat tcaaggttgt
     2701 tgatgttgaa ctgacctact gcatagtggc ctgcctttgc ttttgtaagc atttctttag
     2761 cgtttactaa tggcatttta atcttccttt tttaaaaaat ttcagcagac aagcgtcccc
     2821 acctgccgat ttaagtttat tatacaatat atttttgaaa atataaagac ctaaaaagaa
     2881 gatttagtga aaattttaac aattagtcga aaaattcttc attttgtcct gttttagtca
     2941 attatcacta aactttcaaa ataaactcac acttccgtaa cacaacaagg ttgattttga
     3001 ttttgtgaaa aggtataata taaatgtaaa ttacaatact tgattttggt caagcgttgt
     3061 atgataatat tttttgtaaa ctatcccgta ggggtagtct tgcgaccgca agtaatgaca
     3121 gatattttat tgcaaacaaa gttttataaa tataacaaaa caaccgatat agtgactggc
     3181 ggatgatatc cgcccctacg aaatatttgt tcatttcggc aatttgcttg caaaaaattt
     3241 tgtttcatta cttaaggaca aacgcagttt gtaccccacg gtaacaaata aatgtaggag
     3301 taacttttat ggacaatttt gacaacaaca atatgggtgg gtttgaaccg caaaagccgt
     3361 tcaccgaaaa gcccgtacaa aacaataata tgaacacgga acagacaatt cccgtttccg
     3421 aacccgaaac acaggaagaa caggctcaga atgttcagcc gcagaacggc aacacaccgt
     3481 acggtgatta tattttcggc aacaacaatc aaaacacggc aaacaatgca aatccgactg
     3541 aaaatgttcc gccgtatcag cagaatcaaa atccgaatcc gaacacctac gctcagccgt
     3601 ggcagtacgg ttacggtcag tatccctaca gcggacagaa tccgcagtat aatcagaaca
     3661 atcagcagta cggacagtat caaaacaaca caggatacaa tcagaatgta cctccgtatc
     3721 agcagaatat gtacggacag cctccctatg gcaacaacgg tacatacaat cagcaataca
     3781 atccgcagat gttcgcacag tacccccaaa agaaaacaaa gggcggaata attgcgttga
     3841 ttatcgttct ctgttcactt cttacaatcg gttttatcgg catgatggtg tacggatttt
     3901 cggctgacat aaaagaagat ttgaacaacg accgaagtga ttcgggcaat tcgttcagac
     3961 ttccgaataa agacagcaca acaccgtttg aaacccttcc cgacacatct tcgcagggta
     4021 aaactcacga tgagtcggac tacagtgaca aggtgaacaa ggattactcg ggtatgaagc
     4081 ttgaaagcaa tccgaaagac gcaaaaacaa acaacagcta cacagccgca aaagcgtcag
     4141 aaaaggtttc cgactcggta gtcggcattc tctgttactc ggacgatgta cccgatcagg
     4201 ctgacacaac aaccgcatct tcacagggaa gcggaattat cttctcacag gacggctatg
     4261 ttatcacaaa cgctcatgta atcggcaaca gcaaaacagc ctatgcaatc agagttgtaa
     4321 catctgacgg caaggtatac aaggcaggtg ttgtaggtta cgattcaaga accgatattg
     4381 cagttctcaa aatggacgat gcaaagggac ttactcctgc aacattcggc gattcgtcac
     4441 agcttgaggt tggtcaggat attatcgtag tcggcaatcc cggcggtctt gactatcaga
     4501 acacaacaac caagggcgtt atttcggcgc ttgacagaaa actttcaaca tcaagcctta
     4561 caaagtacat tcagaccgat gccgcaatca atccgggcaa ctcgggcggt ccgcttgtaa
     4621 actactacgg tcaggttgtc ggaattacaa cttcaaagat tgtttccgaa acttacgaag
     4681 gtatgggctt tgcaattccg tcacagacag taaaaagcat tgttgacaca cttgtaaaga
     4741 acggctatgt tgaaggcaga gtaaaaatcg gcatttcggg cattgcagta acctccgatc
     4801 aggcatcaaa ttacaacatt ccgcagggca tttatgttca gagtatcgta tcgggcggtc
     4861 cgtgtgacgg cacaagcctt aaagaaggcg atatcatcac agaggttgac ggcgaaacaa
     4921 ttacaagctt tgcagatgtt tatgcgattc ttgaaaccca caagccgggg gacaaaatca
     4981 aggttaaata ttacagctca tcatcgggcg acggtgaggt tgaaatcaca cttcaggaag
     5041 ataagtaatt ttaatataag aatatcacca aaagcaagag ggcagtcacg gcaatgtgac
     5101 tgccctcttg ctaattgaaa attaaaagta aaaaattgca ggggtgaacc gtgttcgccc
     5161 gttcgtgtgc gatgcacacg attgaacgca tagcgttcaa atatcatatg caactcattt
     5221 atataattta attttgcata tacattgcgt cattattttt taacggggat aaaattccgc
     5281 aaaaatcctg cacttcttcc tttcaggtaa aaccaaccta tggtgtcaaa aactaccgct
     5341 cctacaaaat taacaagcaa atctttcatt gtatcgatca gaccgatgtc aaggtaaccg
     5401 ttaataccga gattttcacc gttcacaacg gttgatgtta tatctctgat gtgcattatc
     5461 acattcggct tgcccgaaat caaacccgaa tgtattgccg tgattacggt gtccttttgc
     5521 atatcctttt caaaaagcat atccgcacca aactcaaaaa actcccacac aacaccgact
     5581 gtcattgaaa agcagaacga aaacaggcac acaaacatgg gcgagaggct caatttcacc
     5641 cgctcgcttc tgttcaaaat atcggtcagt ccaaaaccaa cgcctgcaca gataaagccg
     5701 ttcacagtat ggagcaatga atcccatatc ggaatctttt cgtagaacgc accgagctca
     5761 cccataacat ttgccgcaaa cacgaacagc acaacaatcg tttcaagcac actcggcaac
     5821 gctaccgaca gctttcggtc gatgaacaac ggaagcatca acagtccgag cgttaacacg
     5881 caggtcatga cattctccca ttttccgttt aaaatgccgg cagtcaaaag tgacacaagt
     5941 atgagcctta taatgatgta aacgcctgcc ttaaccttgt gctgtcgcat ttcacgcaca
     6001 ttcagccacc gtgcaagcct ttgaaaaaat cctttaagtc gttttttcat aatatccccc
     6061 gatttttaca cttatttttt ccttaaaaac gagataaacc cctgtaaaat tgtattagct
     6121 ttttctgcct gatattaccg caaaataaaa aagccaccgc catctgcgat ggctcataag
     6181 cattgatatt aaattacttt ttctctgact tctctgacga ttcggctttt tcgccatttt
     6241 tttcagcctc aagctttttc ttgagttcct ctttgccgac agcaagcatc agcttgatac
     6301 ggttctcctg gttgaccttc ggagcacccg gatcatagtc gatggtaacg atattggcat
     6361 tcggtctgat ttctttaatc tttctgattg cgcccttacc gttgatatga ttcggcagac
     6421 aaccgaaagg ctgtgtgcat acgatattct catagcctgt atcggcaagt tcaatcatct
     6481 cggctgtcag caaccagccc tcacccatct tactgccgta gccgataaca ccgtttacaa
     6541 gctcctttgt gtgagcatat ctgtgaggcg gaacaaagcc gtacttttca gcggcatcaa
     6601 ccataagtcc ctcaagcttt gtgaggtagt taagaagcat cgaacagaat ttgtatttaa
     6661 ctctgcttcc tccgaacatc ttgtagtcct caatacggtt atcaacctta aatgatgcaa
     6721 aacccataag accgggtacg caaacctcgc aatcctgatc tgcaagaaag tcctcaagac
     6781 cgttgttagc cattttggag tattttacat agatttcgcc gacaataccg actttaacct
     6841 tcggcacttt gttaagctca atttttgtga atgactttgt aattttatca agagtttcgt
     6901 caatttcttc aagagtgtag cccttgccct gtatgagcat atcggaaatc ttatccgtcc
     6961 actcatcaac aagcgccata ctttcgccct tgttcacctc atagggcttt gtctggtttg
     7021 aaagcagcat taactggtcg ccgtacacaa gaccgccgac aaatctgcgg ataagcggaa
     7081 gtgtgagcga aaagccgctg ttgtgctcca taccgaacga aagtgaaatt acgggaacga
     7141 actcaaagcc tgcctttttg agagcctttc tcaaaaggaa aatgtagttt gacgcacgac
     7201 atccgccgcc tgtctgagta atcatcaggg caaccttgtg aacatcatac tttccgcttt
     7261 gcagagcatt gatgaactga cctataacca gcaaagccgg ataacaggta tcgttgtgaa
     7321 catatttaag acctgtctgc gcaatctccg gacccgttgt gtcgagcaaa tcagacttat
     7381 agccgtagta gttgaacacc ttttcaagta ttttaaaatg aataggcgcc atattgggca
     7441 taagaattgt gtattccttt ttcatctgct ttgtaaacag caaacgacct gtttttttgt
     7501 catacttcaa ctctgccata acagaatttc ctttcatcaa tcgttttcaa gcgcagcaag
     7561 caaactgcgg attcggattt taactgctcc gaggtttgta atttcatcta tcttaatctg
     7621 agtgtaaatc ttatttttgc tttcaagaat ttctcttacc tcatcggttg taatcgcatc
     7681 aacaccgcag ccgaaggata caagctgaac aagctccatg tcctttcttg ttgtaacata
     7741 ttttgccgcc gcataaagtc ttgcgtggta tgtccactgg ttgagaacat gagtccggaa
     7801 atgttcaact ctcggagaaa taacatcttc gctgacgatt gcaacatcaa agcctgagat
     7861 aagtctgtcg attccgtggt taatttcggg atcaatatga tatggtctgc ctgcaagaac
     7921 aaaaatcttt ttgccctctt tttcagcctt ggcaataatc tcgttgccct tgtcaataac
     7981 cttttttctg taattttcct gttcctgata tgcaagctta gccgcatttc ttacctcgtt
     8041 aagagtgagg tcggggaagt ggaacgaaag tctttcatag gctttcttag gaaaatcgtt
     8101 cgggcggtga ataccaaagt attcctttat gaagtgaacc ttgcgtatat ccttcatatt
     8161 gtttttgata acctcggggt agtaagcaac aacaggacag ttgtaatggt tatctccgag
     8221 atgctcgtca aaattatagg aaagacaggg gtagaaaatt gtttctgcac ccatatcaat
     8281 aagagtctga atgtgaccgt gaacaagctt tgcagggaag caaacggtat cgctcggaat
     8341 tgtctgctga cctctctgat aaagctttct tgttgaatac ggtgagtgga acacctcaaa
     8401 tttaagttct gtaaagaatc tgtaccagaa cggataaagc tcatacatat tgagcgccat
     8461 aggaattcca agcttacctc ttatgccttc aacaggcttg taggaatcaa tgagattcag
     8521 cttataggca tacatattca tatcgtttgc aggagctttc tttgtgattg gtctttcaca
     8581 acggtttccc gcaataaact ttctgccctt gccgaatgaa tttattgtaa gacggcagtt
     8641 gttgttgcac ataccgcagt ttgcaacctt gatttcgtga acgaattcat caagtccctt
     8701 tttgtcggta attgtactct tgccaacgcc ctttgacttt ttctttgcgt aaagagccgc
     8761 accgtaagca cccataagtc ctgcaatgtt aggacggaca acttcaacgc ccatttcctg
     8821 ttcaaatgca cgcaaaacag cgtcgttaag gaaagtaccg ccctgaacta caacacgctt
     8881 gccgagttcg tcggggcttg acgcacgaat aaccttgtaa agggcattct ttacaacgct
     8941 gagtgaaaga cctgccgaga tatcttcaat tgttgcgccg tctttctgag cctgcttaac
     9001 gcttgaattc atgaatacgg tacagcgtga gccgaggtca acgggacgct ttgcaaaaag
     9061 tccgagcttt gcaaagtcgg caatctcata tccgagagcg tttgcaaaag tctgcaaaaa
     9121 gcttccgcaa cccgatgaac aagcctcatt aaggaatata ttgtcaattg caccgttatg
     9181 tatcttaaag catttgatat cctgtccgcc gatatcgatg ataaattcaa catcgggcat
     9241 aaagtatttt gcggcggtaa agtgggcgat tgtttcaaca atgccgtaat caaccgcaaa
     9301 tgcgttcttt acaatgtcct caccgtaacc tgttacggca gaagaaacaa catttatttc
     9361 gggatttatt tcgtaaacct cgtcaaggaa ttgcttcata atctcaacgg ggtttccctt
     9421 tgatggcaga tattttgaat agagcagctc accgtcatca ttgagaacaa caccctttac
     9481 ggttgttgaa cctgcgtcca ttcctatata agccttgcct gtgtagccct ttagctcctt
     9541 ctgcttaaca tcagcctttg catgacggtc gcagaattcc ttgtactcct cttcgttttt
     9601 aaagagcggc tgattgaacg caaagttacc gctgccacgg tagttcttta ccttttcgat
     9661 aacctcgtca aaatcaatag tcttttcggc acacaaagcc gcaccgcagg caacataata
     9721 aagtgaattt tcggggcaga tgccctttgt tccgagcgtg cggtcaaagc agtttcgcag
     9781 ttcgctcata aatgtaagag gtccgccgag atatacaatc tgaccttcaa tttctctgcc
     9841 ctgtgcaaga cctgcaacag tctggcttac aaccgcattg aaaatactct ctgcgatgtc
     9901 gctcttcctc gcaccctgat taaggagcgg ctgaatatcg gtctttgcaa acacaccgca
     9961 tcttgatgcg attgtgtatg tcttttcgtg ctgttttgca agctcgtcca tatcctcaag
    10021 cttaacattc agaagtgttg ccatctggtc aataaacgca cccgtgccgc cggcgcaggt
    10081 accgttcatt cttacctcaa gtccgtttgt gagaaacaat atctttgcat cctcaccgcc
    10141 gagttcaatt ataacatctg tacccggaat aaaagtattg gcggcaactc ttgttgcgta
    10201 aacctcctga ataaaatcaa cgccgacaga ctcggcaaca cccattccgg cagaacccga
    10261 aagagccacc gaggcatttt caacgccgtc aatccttgtc cgtacaagtg caagaatttc
    10321 tgcaatcttt tccgtaattc gggaataatg cctttcatat gtgctgtata ttaatttgtt
    10381 ttcgtcgtca agcacaacac attttattgt tgttgagcca atatccaatc ctaatttcaa
    10441 cttgtgcctc cgaaatcaat acatagtaat ctcattgtgc atatctaatt aattatagta
    10501 ttttttgcca agaaaataat tctaattcag aatattttca cctttgatga attttaccat
    10561 ttttatcaca aaaattcaat acacataaag ggcataatgt ataatctact gcatataatt
    10621 tgccctcaaa agcaaagcgg agcattgttc cgactttaat atatcttaaa aatgcagaaa
    10681 aacctgccga aatcggcagg tctttagctt taagtattta aattatcaga aaagttcacc
    10741 gtgaggagct tcaccgtcat ctgcctcgtc tgaatctgcg gactcggaga atccttcttc
    10801 acgctttgcc tcaagctctg ccttgaggtc gcagatgttt gtaagatgct cgggttctgc
    10861 cttttcttcg gtaacagcct cgggctgttt ttcaacaggc ttttcctctt taaaaggaga
    10921 tgacttgtag tcgccctcat ttgagcaagc tactgtaata tcaacaatat catcctcttt
    10981 aacctcggga gcaacctcgg gattgccgta gaaaatatcg tttgagctgt tgacaatctt
    11041 tctgtaacac tctcttgctt ttgcgatttt ggcctttgaa gtgtcaataa ccttcaaaag
    11101 cttttcgcac tcggcgtcat taatttcagt gcctttcttt ctgctgtcgt acataatctt
    11161 tccgagagca atatatgctc tgttcataag ctcgctttcg cttttcataa caagtcttaa
    11221 tctgttgagc tttgcgtttg ttctgttctt ttctactatt gtctgagcta ccgatgaaat
    11281 tgtatcaacg gctgtattta ctgttccttt tacactgtca agtatcgtca tagtaaattt
    11341 ctcctttaaa accgctttgg cacaaacact ctgacacatt ctgccgtggc gaataatatt
    11401 aaacttattt ctattattat gacagatttt tttgataaaa tcaacatttt tttaaaattt
    11461 acttgttaaa atcacggtca attgctataa ttagtttcat agagtgttaa agaggtggtt
    11521 tggatgcctg aaaagaaaat taccaaggca atcgtctgcc caatgtgcgg tgaaatgagc
    11581 aaggctgatg tttacaccag catcaacccg tccgtaacaa agggtgtgcg cagaagagcg
    11641 cttgacggtg agctttttgc gtggaaatgt ccgtcatgcg gatactccgc aaggctcaca
    11701 tatccgattc tctacaatga tatgaagaat cgttttatgg tgtatttcat tcccaaaatt
    11761 gatcattttc agctctgcga caacgagctt gaagaaaaat acagcaatct ccgcaacata
    11821 agcaaacgaa ttgttccgac cttcaattcg ttcaaggaga aaatttttat ctttgagtcg
    11881 ggacttgacg atatgtcggt tgagcttaca aagcttgcaa tcagccaaac ggtttcaaaa
    11941 aagcttaacg gtgcggaaat tcatgacgga tacctttcaa tgtacgatcg tgaacgcaac
    12001 acaatgggat ttacatattt tacaggtgaa aagcatacgc cgtatgttca gacagcaaga
    12061 cttgaaattt atgttaaatc gaagaaaatt gtcgattcgc ttgcatataa ggacaaaaag
    12121 ttaaagggct ttcttaaaat tgaccgtgaa tgggcagaaa acattctcta ccgctacaag
    12181 cgcatgaagc atattgaaaa gcttaacaag ctgaaagagt aagctgttac tgtagtcagt
    12241 cgattctttt ggtttaaaaa agaatctgca aataagtaac atatacactg taaagaatat
    12301 ccgtaaacat aaactcgtta aagcaaacat gcataaaaat ttccgccatt acgattgggg
    12361 cggcggaatc attcagctta atattttttg tatttgcaat tccaacttgt taaaatgccg
    12421 atacaatcgg ggattttcgt tgcacaaaaa atcagggatc gactcaaaga gccgacccct
    12481 ttttaattta ttgaaaagat tggtaattac ttgttaacag cttcataaac tgcaactgcg
    12541 caagcaactg tagcaccaac catcgggttg ttacccatac cgataaggcc catcatttca
    12601 acatgagcag gaacagaaga tgaacctgcg aactgagcgt caccgtgcat tctgcccatt
    12661 gagtctgtaa gaccgtatga tgcaggacct gcacccatgt tatcggggtg aagtgtacgg
    12721 cctgtaccgc cgcctgatgc aaccgagaaa tacttaacgc cgttctcatt tgcccacttc
    12781 ttgtatgtac ctgcaacgag gtgctggaaa cgagtcgggt tagtagagtt acctgtgata
    12841 gatacaccta cattttcatg ctgcatgatt gcaacacctt cacgaacatc gtcagcgccg
    12901 tagcagcgaa ctgcagctct ttcaccgtct gagaaagctc tctcttcaac gattttaagc
    12961 tctgatgtga aatagtcaaa ctgagtcttt acatatgtaa agccgttgat tcttgaaata
    13021 atgtaagcag catccttgcc aagaccgtta aggataacac ggagcggctc ttttcttgct
    13081 ttgttagcgt tacgagcgat accgatagca ccctctgctg ctgcaaagct ttcgtgacct
    13141 gcgaggaatg caaagcactt tgtttcatct ctgaggagca ttgcgccgag gttaccgtga
    13201 ccacggccaa catttctctg ctcagctact gagccgggaa tacagaaagc ctcaagaccg
    13261 ataccgatag cttctgctgc ctctgaagca gtctttgcgc ccttcttaac tgcaactgca
    13321 caaccgagtg tgtaagccca gcctgcgttt tcaaatgcga tcggctgaac gccctttacg
    13381 atgtcgtagg gatcaaaacc gagatcgtta caaatttttc ttgcctcttc aagatttgca
    13441 ataccgtatt cggcaaggca cttctcaatt ttaggcattc ttctttccat accttcaaat
    13501 gttactgcca ttatggtgtc ctccttaccg aattattctt cacgagggtc aatgtactta
    13561 gctgcgccgt caaaacgacc gtactgaccg atgttgttct tgtatgcttc gtttggctca
    13621 acgccgtgac gaatgtcctc gagcattttg ccgagcttta cgaactgata gccgataact
    13681 tcatcgttct cgtcaagtgc tgtcttgaga acatagcctt cagccatttc catgtaacga
    13741 acgcccttag cgtttgtgct gtacattgta ccaacctggc tgcgaagacc cttaccgaga
    13801 tcctccatac ctgcgccgat tggaagaccg ccctctgaga aagctgtctg gcttctgccg
    13861 taaacgagct gctcgaagat gttcttcata gctgtgttga tagcgtcaca aacgaggtct
    13921 gtgttaagac actcaaggag tgttttgtta ggaagaattt ctgctgccat agctgcagag
    13981 tgagtcatac ccgaacaacc gattgtttca acaagagcct cttcaacgat accgtcttta
    14041 acattaagtg taagcttgca ggcaccctgc tgaggagcac accagccaat gccgtgtgaa
    14101 agacctgaaa tgtctttaat gtcataagat tttacccatt tgccctcttc gggaataggt
    14161 gcaggaccgt gcttaggtcc ctttgctaca gtgcacatat tttcaacttc ttttgaataa
    14221 atcatgtgta cttctccttt caataaattg tatgagtccg aaatcctcaa acacctatat
    14281 tatacggctt aacccgacat ttttcaagca tttttcgtta atttttacaa ttcccccaca
    14341 ttgtaatatt tgccgtggca attttccgtt tgattttgaa aaggtaaaaa taaaaacggc
    14401 aaccgtgaaa caaatcaccg attgccgtta aagtttactt attcttccca aaacaattcg
    14461 tcaagggttt tgtcaagcgc tttacatatt gctatgcaaa ggttaattgt gggattgtag
    14521 tcgccctttt caattgcgct tattgtctgt cttgacacct ttacaagttc cgcaagctgt
    14581 tgctgtgaca aatcctttgc ggcacgggcg ctttttaatc ttaaattctt cattcttcaa
    14641 ctgccttttt gtcaattatt tttttgatga caactatcac aaaaatactt attgtccaaa
    14701 tcattacaat gataggaata atgtcaagat ttgctattcc gtttgtaaca aaggttttgt
    14761 tgccgaaagc aattcccaaa cattctgctc ccgcaacaaa tgccaaagag cctaaaagac
    14821 tccttctgcc tttattcgat gtgaaatatg cgtcatttaa aatgcttatt ccgacgaata
    14881 cggcggttga aagaaacagt cctatgaaca tttgaacgct gagttcagcc caattaatct
    14941 ccaaaaca
//
LOCUS       DAOMVE010000030        14921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_846_length_14921_cov_2.785215, whole genome shotgun sequence.
ACCESSION   DAOMVE010000030 DAOMVE010000000
VERSION     DAOMVE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14921
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_14921_cov_2.785215"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="PLS64_02935"
     CDS             complement(<1..912)
                     /locus_tag="PLS64_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42821.1"
                     /translation="MRATNYKHYGCDNNSESSKRTRKPEKKSKRKGFKALSYAIAVSL
                     AAAALVVPTTTLAPDVDAFEDSRAASLAVGNINDFSLVITDNCVEETTAPVTQETTTS
                     AATTQKTTEKETKSAKTAKKKTESSQASKKAESLKSEPSESETSQSYTSYVDSSSDNS
                     SSASNSSDYGYTDSTSSSSNSQSGYLVSIANPDPSYSPSPVSLSSYDREKLERLVMGE
                     GGSMGYTGCALIAQSIRDAMNRSNTTSIDRIISEYQYYGSTDYAPNQDVKDAVSFIFD
                     QNGSAVQHRVLCFYVGSSSWHESQNYIT"
     gene            complement(1242..1529)
                     /locus_tag="PLS64_02940"
     CDS             complement(1242..1529)
                     /locus_tag="PLS64_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HRL42822.1"
                     /translation="MNCRFTDLRHKEVISACDGTRIGFIDDVIVDTKCATVVSIVIFG
                     RPGFFSLVGKCQDYIIPWEKIDLIGEDTVIISCQTPKPVKKPKKPRFGAKF"
     gene            1659..2531
                     /locus_tag="PLS64_02945"
     CDS             1659..2531
                     /locus_tag="PLS64_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRL42823.1"
                     /translation="MNYVFMTDASCDLTQELVNEIGVEVLPMEFHMEDKSYLHYPDCR
                     MMSLSEFYSKLKDGIDSKTTQINYDSFYKSFENYLKAGKDIIYTGISTGLSGTYNTCM
                     MAVKDLKEVYPDRKIIAIDSLCDSAGLGLLIYLAGKKYSEGATIDELKEYIEDTRIKV
                     CHWFVVEDLDQLKRGGRISSVSATFGKALQIKPLISVDDEGHLINVAKIRGKSNVVPA
                     LVKHLERDAENLKDSVVMVAHADNPEGAEELKKAIKGKCKEVVMTDIGPVIGTHVGSG
                     MLAVLFLGTRNLKS"
     gene            2798..3601
                     /locus_tag="PLS64_02950"
     CDS             2798..3601
                     /locus_tag="PLS64_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HRL42824.1"
                     /translation="MKSVYIDVLITVNVFIDFILILCTKKALCINTSFKKMLLASLLG
                     GVQSLIALFPPLPFFLNIPIDVLCAAGIVLCAFGKCPFKCFIKRISVFLSLSFSFCGI
                     MMFLYNAFKPKGMEVYNDTVYFNISPVLLIILTLVCYYILKLTKILTKGGNGSGVCKV
                     EIKVNEQSYFFPAKIDTGCNLVEPFSGNYVIIAEQNIFDDYVPDETKTRIIPFESLGG
                     NGIVKGFPPDKVKIDGKEICKSIYIGICNDVLKGDVKAIIPYELVKISE"
     gene            3612..4313
                     /gene="sigE"
                     /locus_tag="PLS64_02955"
     CDS             3612..4313
                     /gene="sigE"
                     /locus_tag="PLS64_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HRL42825.1"
                     /translation="MFLMKVIQKIRFALVIENEIFYINGSETLPPPLTREEEQHIMEN
                     IAKGDSKAREPLIVHNLRLVVYIAKKFETPNASAEDLISIGTIGLIKAVNTFSPEKNI
                     KLATYASRCIENEILMFLRKSSQLKNEVSIDEPLNTDWDGNELLLCDVLGSDPDTVNR
                     NIECELEKSLVLQAVSKLRDRECLIMELRFGLNGRKEHTQKQVADKLGISQSYISRLE
                     KKIIRQLKIDLDKAV"
     gene            complement(4396..4719)
                     /locus_tag="PLS64_02960"
     CDS             complement(4396..4719)
                     /locus_tag="PLS64_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42826.1"
                     /translation="MKKFLGLFAVALISVCLITGCGNDKNDSMTDTTSQTSQTSNTQE
                     TSKSMTENGEVSDSDGFIGNEENETGNSGSDMMDDIEDGIDDIMGDGDNNDSDTVTNP
                     SDTVL"
     gene            complement(4817..5605)
                     /locus_tag="PLS64_02965"
     CDS             complement(4817..5605)
                     /locus_tag="PLS64_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRL42827.1"
                     /translation="MNITEEKLLEYLSKALVCVAVIVIGYIITRLIIGILRKILNKSR
                     MDGTAEGFVLSVLKVIFYFIVAVTALGTIGVNVASLITALGAAALTAGLALQDLLKNV
                     VSGLVILINKPFVSGNILDFEGVKGTVEEINIFSTTVHTLDNKLVTIPNSRLTSNNVV
                     NCTMVEQRRVDCTFSVSYDDDIDKVREIILNLMATNEMILKDPEPTVRVGEHLDSGVQ
                     IKVFAWATPDDYYEVYFFLQENVKKEFDKNGITIPYPHLVIAKD"
     gene            complement(5608..7242)
                     /locus_tag="PLS64_02970"
     CDS             complement(5608..7242)
                     /locus_tag="PLS64_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42828.1"
                     /translation="MLEQSQKLTDGLIIYSVLVSDYEKLDFKNDALAGVRANYFNSGC
                     KEQKEITEIELHGMDYKMSCLLTKNEPLSWKVTRDNLPYQSVKRATGGVYSVITYNDR
                     GIVFKRQFFDRNHTWLRTEYFNRSIADKLITRIYPRKVSGIITLVTEDIDDNGIVSVK
                     TLFPSDKQSNENSRVLIYSNVGMLWYDASFLPSDMPFTEKKSYGNSGFVFNGDLFKND
                     FVPENAYNLEDCAYLTDEDIPNVSDTAEPVSKKDYSAYDIIEKILVEAHKTNKDLFGE
                     IINHTAEEDFEPAEVKIVTGEKDEQLSDDEVEENSQISEHEIADDTDAVAETADEQTE
                     DSENVVTVDSSDEDSENEETDFENVEANIGTHCQIDDVPTGESDEENPEIVNEEEPHC
                     DVVILTKSGRYTYFGNLDENNCRTGRGRTVSPDGMTSYDGEYLDDRRNGFGVCYYNNG
                     SINYVGNWTDNSRNGGGVGYRLSDGTMHAGKWDNNTPDGYGARFDRNGNLIDVSNYEN
                     GVRTGKSVSFDENGNIVVSVYENGEKISEFLVDAEV"
     gene            complement(7342..>7828)
                     /locus_tag="PLS64_02975"
     CDS             complement(7342..>7828)
                     /locus_tag="PLS64_02975"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRL42829.1"
                     /translation="HDRCSIVEVMGRRCGDIALQTGIATGATAILVPEVQYNIERDVI
                     ARIINTQKTGKKHFIVVVAEGVGGVRDLAKYVEQRLGIEARATVLGHVQRGGSPTLRD
                     RVVASQMGFKAVELLEQNIGNRVVAMQGGKIVDLDINEALDMPRVFDEELYKIAMTIS
                     I"
     assembly_gap    7829..7838
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7839..8302)
                     /locus_tag="PLS64_02980"
                     /pseudo
     CDS             complement(7839..8302)
                     /locus_tag="PLS64_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
     gene            8639..9592
                     /locus_tag="PLS64_02985"
     CDS             8639..9592
                     /locus_tag="PLS64_02985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL42830.1"
                     /translation="MTKLYLAIPCYNEEEVLRDSAEKLLNKYSTMMAEGKISADSRIV
                     FIDDGSKDRTWEIISELHTENPIFQGIKLSRNRGHQNALLCGLMTLKDKADAVISIDA
                     DLQDDINTFDEMVAKYEDGCDVVYGVRSKRETDTFFKRFTAEGFYKILNHMGAKVIFN
                     HADFRLMSKRALNAFSLYKETNIFLRGMVPMIGFKSDIVTYERSERLAGESKYPLKKM
                     LALAWEGITSLSIQPIRMITWLGAIVFGISLIMIIYSLISFFCGWTVSGWSSLLCSIW
                     AIGGLQLLAIGVIGEYIGKIYMETKRRPRFIVDKVLDDDEQ"
     gene            9582..11168
                     /locus_tag="PLS64_02990"
     CDS             9582..11168
                     /locus_tag="PLS64_02990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRL42831.1"
                     /translation="MNSNRITKVLTGIFGGVFTVCFVFLFVNIIIIGFDGNVDRRFDS
                     FSKMFILLIFAVLTVGICALYFHYTSDKSCKKIKAKSRFEFNDKNTKNVIFIGCGILL
                     IVEIIFALLTDFEPVADLHNIKRYAMYFSTHGNFNLIEQDYANDYQYLVRYPNNMALL
                     LIVSLVGRLNYLIFGHYVDFAPVVVNIFAINISIMLTAFTAKRLFGNKKALFVLAFCA
                     LFLPYLTYLPYYYSDSMSMPFLIGAVYLIVSALQVDNRKSMYAKLCAAGALIFLGYKV
                     KGSLIILFAVGLLLLFLKFRLKKAICLILVFTAGFGVIGFAYNTAVDAVNPITKQQYE
                     KYEYPVTHWLMMGLKGLGKYDEHDDYYTRSFPSKKEKQDANIKVIKERIKDYKFSGLY
                     EHMIEKAVWTWQDGTYYISYHNRKPKNDNILMDFLHIDGKYNKAFQNYSSALQLFILL
                     MICISAVKTLIKPKVDEMLLIKGVVFAAFLFFLLWETRSRYLFDMTPLFILLTVDGMD
                     TAKAFLDSFRKPKQQTEKQA"
     gene            11361..>13092
                     /locus_tag="PLS64_02995"
     CDS             11361..>13092
                     /locus_tag="PLS64_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRL42832.1"
                     /translation="MDYEKLKKRDTSLDILRIIAVFTVLSVHFFLHNGFYSQTIEGTP
                     MYVAVVMRTLFSVCVPLFMLLTGYLMSKKELSKKYYSGITKTLVVFVISTLACMIYKN
                     IAQGDVFDLKSFILGTLDFTGSNYSWYIEMYIGLFLLAPFLNLAYGKLKSKKQKQVLL
                     VTAVFLTIIPSLFNIFNFGSLDWWTNPTSSDEFQKLVPSWWQGFYPVAYYFVGCYIRE
                     YGLKMKTRTMLVLFVFSLFIFSTFNYFRSYGTTFKSGTYIYWYGFEPFVLSVLLFLLI
                     KRIKTDNFPKAAKIALWKVSDLALGIYLISFIFDSIVYPVLCEKVILMPDRLPYYFVT
                     VPIVFVLSAAASFIMNLVAKLLIDGFKSLANIIKDLRSKPDKGKWQHIIFAVLMAFAI
                     GFSLWKCYYGFGGNDESFYLTIPHRFTLGDSLLGDEWHLTQLSGFLLLPFVWLYTMIT
                     QSTVGIIFAARVFYVICHAVIVCVIYSRLKKYGYFSVFGCVLYFLFTPFDIMALSYNT
                     MGLDLIALTGVLMATADYSKKLPLIISGLAFAGAVLCCPYLAAVYVMYIIAIGVHYVI
                     KKTALNKNVFN"
     assembly_gap    13093..13112
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13113..14200
                     /locus_tag="PLS64_03000"
     CDS             <13113..14200
                     /locus_tag="PLS64_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42833.1"
                     /translation="FTLGVGILVAIFLVFVLSRVSINEIFSNLPYLLADPDHPQMGFM
                     AKMNYYFKTIVECHTHFKYVLMAYGATAIVMLLDRKRKQHRSIYLILTSAIVILSLVM
                     FMPTMTSVYYNAIMFPMIFMGITAYVLSENKQRELFASLFVLGIFYSVALCFSSNQYF
                     YVTAMACTASNIASFVFIGNLIKEMKANPDNLDYAVPCKYLAFVMTAFLIILQACFQI
                     TVKAEHCFWDSEPKQLTQTIQNGPAKGIKTTPNNAQTYEQIYADISQYQNLEKGNILF
                     LTQKTWTYLAAEDFPYGTLSAYVTGENQNSLARLRSYYSVNSKKIPKYIYIPKDSEWD
                     NLQQILLEAQQNGYSLSENEVSYKLVK"
     gene            14421..>14921
                     /locus_tag="PLS64_03005"
     CDS             14421..>14921
                     /locus_tag="PLS64_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRL42834.1"
                     /translation="MDIKQTYNEWLENAVEDKDLTAELESIKNNEDEIYDRFYTALKF
                     GTAGLRGIIGAGTNRMNIYVVRQATQGLANYVLKKYGNGSVAISHDSRIKADLFMNEA
                     ARVLAANGIKVYITSELQPTPVLSYLVRYFKCQAGIMVTASHNPAAYNGYKAYGESEE
                     GADQRVA"
BASE COUNT         4415 a         2917 c         2826 g         4733 t
ORIGIN      
        1 tgtgatgtaa ttctgtgatt catgccagct gcttgaaccg acatagaagc aaaggactct
       61 gtgctgaact gccgagccgt tctgatcaaa gataaaggaa acagcatctt ttacatcttg
      121 attcggtgca taatcagtcg aaccgtaata ctgatattcc gaaataattc tgtcaattga
      181 tgttgtgttt gaacggttca ttgcgtcacg gattgactgt gcgataaggg cacagcctgt
      241 gtaacccatt gaaccacctt cgcccattac aagtctttcg agtttctctc tgtcatagct
      301 tgaaagagaa acaggagaag gactgtagga cggatccgga ttggcgatac ttacaaggta
      361 gccactctga gagtttgacg agcttgatgt actgtctgta tagccgtaat ccgatgaatt
      421 agatgcggaa ctgctgttgt ccgatgaact gtcaacatag cttgtatagc tctgtgaagt
      481 ttctgactcc gaaggctctg actttaaact ttcagccttt ttggaagcct gtgaactttc
      541 ggttttcttc ttcgcagttt ttgcagattt agtttccttt tcagttgttt tctgtgtagt
      601 cgctgcggat gttgtagtct cttgggttac gggggcagta gtttcttcaa cgcagttatc
      661 tgtaataacg agtgaaaaat cattgatgtt accgactgca agcgaagcag cacggctgtc
      721 ttcaaaagca tctacatccg gtgcaagagt tgttgtcgga acaacaagag cagctgccgc
      781 cagagatacc gcaatagcat aggataatgc cttaaagcct ttgcgttttg acttcttttc
      841 gggtttgcga gttctctttg aactctccga attgttgtca catccgtagt gtttatagtt
      901 tgtcgctctc aaaaatttat cctccctgtg atttttgcac attaaggtaa ctactataac
      961 tgccgtcaaa agcaagcgta tatacaagat gtagttatct gtttaaaaat gtgccaagtc
     1021 ctttagtcgt aaaattttat caaacacaca attgcaattg tattaaagca caaacttttg
     1081 aaaattgcaa gtttgacagg ggaagataaa ttgtaacaat tgtaaccgaa atgttactta
     1141 tgtggtttta atttcttaaa aaaataatga tttgtgcatt atgcataatt tagcaaatat
     1201 ttcttaggtt attttgcctt aatgcgaaaa tttgaaaaaa ttcaaaattt agccccaaaa
     1261 cgtggttttt tcggcttttt tactggcttt ggagtctggc aactgatgat taccgtatcc
     1321 tctcctatga ggtcaatttt ctcccacgga attatgtagt cctgacattt tcccaccaga
     1381 ctaaaaaatc ccggtctgcc gaaaataaca attgacacaa cagttgcgca tttggtatcc
     1441 acaataacat cgtcgataaa tcctattctt gttccgtcac aggcgcttat gacttcctta
     1501 tgtcttaaat cggtaaatct gcaattcata taaccactcc gaaatctgtt tttgatatac
     1561 atatatatga aaaggaacgg tcattatatt cattgacatt tatatcataa tttgttagaa
     1621 ttgaatcaca atattttcgc aaaaaaggag cgataattat gaattatgta tttatgacag
     1681 atgcctcctg cgaccttaca caggagcttg ttaacgaaat cggcgttgaa gttcttccga
     1741 tggaatttca tatggaggac aagtcctatc ttcattatcc ggattgcaga atgatgagcc
     1801 tctcggaatt ctacagtaag cttaaagatg gtattgattc aaagacaaca cagattaact
     1861 atgacagctt ttacaagagc tttgaaaact atcttaaagc cggcaaagat atcatttaca
     1921 caggcatttc aacaggtttg agcggaacat acaacacctg tatgatggct gttaaggatt
     1981 taaaggaagt ttatccggac agaaagatta ttgcaattga ttctctttgc gattcggcag
     2041 gacttggact tctcatctac cttgcaggca aaaaatacag cgaaggcgct acaattgacg
     2101 aactaaaaga atacattgaa gacacccgta taaaagtatg tcattggttt gttgttgaag
     2161 accttgatca actcaagaga ggcggtagaa tttcctctgt ttccgcaaca ttcggcaaag
     2221 ctttgcaaat taaaccgctc atcagtgttg acgatgaagg tcatcttatc aatgttgcaa
     2281 aaatcagagg taagagcaat gttgttccgg ctcttgtaaa acaccttgaa agagacgccg
     2341 aaaatctcaa agacagcgtt gttatggttg cccatgcgga taatccggaa ggcgccgaag
     2401 aacttaaaaa ggcaatcaag ggtaagtgta aggaagttgt catgacggat atcggtcctg
     2461 taatcggtac tcatgtaggt tcgggaatgc tggcagtcct attcctcggc acaagaaacc
     2521 ttaaatcata atcaaaatac agcaaaatat gtaataagcg tgcagtctga attaaactgc
     2581 acgcttttct tttgtcagtc ccttgtgtaa tataaaaggc acattacact tacgccgaca
     2641 aatccgccta taaacaatcc gataattaat cctgcaatca ataatttacc tcctgaccat
     2701 attttgacaa ttattatttt ccgcttgtga gataataagc aaaggtaata aatttaatgg
     2761 aaatgttatt agaacagcat tccgcagggg gaataaaatg aaatctgttt acatagatgt
     2821 actgattacc gtaaatgttt tcattgactt tatacttatt ttatgcacca aaaaggcgct
     2881 ttgtatcaac acctcgttta aaaaaatgtt gcttgcgtcg cttctggggg gagtgcaaag
     2941 tcttattgca ctgttccctc ccctgccttt ctttttgaat atacctatcg atgttttatg
     3001 tgccgccgga attgtgttat gtgcattcgg caagtgtccg tttaagtgtt ttatcaaacg
     3061 aatatccgtg tttctttctc tctcattttc attctgcgga attatgatgt ttctgtacaa
     3121 cgctttcaaa ccgaaaggta tggaagtata caacgacaca gtgtatttta acatttcgcc
     3181 cgtcctcctg attattttaa ctcttgtctg ttattatatt ctcaaattga cgaaaatatt
     3241 gacgaagggg ggcaacggtt cgggagtttg caaagttgaa atcaaggtta acgaacaaag
     3301 ctattttttc cctgcaaaga ttgatacggg ttgcaacctt gttgaaccgt tttcgggaaa
     3361 ttatgtcatt attgctgaac aaaatatttt tgatgattat gttcccgatg aaaccaaaac
     3421 aaggattatt ccgtttgaaa gccttggcgg aaacggaatt gtaaaaggat ttcctcccga
     3481 taaggttaaa attgacggca aggaaatctg caaaagcatt tatataggca tatgtaatga
     3541 tgtattgaaa ggagatgtta aggcaatcat accgtacgaa cttgtaaaaa tatcagaata
     3601 ggagcaatgt tatgttttta atgaaggtta ttcagaaaat ccgatttgca ctcgttattg
     3661 aaaatgaaat attctacatt aacggcagtg aaactctccc cccaccgctt acacgagaag
     3721 aagaacagca tattatggag aatattgcaa aaggagacag taaggcaaga gaaccgctta
     3781 ttgtccacaa tttaagactt gtggtatata ttgcaaaaaa gtttgaaact ccgaatgcaa
     3841 gtgcggagga tttgatttca ataggcacaa tcgggcttat caaggcggtg aacacctttt
     3901 ctcccgaaaa gaatattaag cttgcaacct acgcatcaag atgtattgaa aacgaaattc
     3961 tgatgttttt acgaaaatca tcgcaactga aaaacgaggt atcgattgac gaaccgctga
     4021 acacggactg ggacggcaat gaactgctct tatgcgatgt tctcggaagc gatcctgaca
     4081 cggtaaacag aaatattgaa tgcgagcttg aaaaaagtct tgttttgcag gcagtttcaa
     4141 agcttcgtga ccgagaatgt cttattatgg agttacgctt cgggcttaac ggacgaaagg
     4201 aacacacgca aaagcaggtt gccgacaagc ttggaatttc gcagtcctac atatcaaggc
     4261 ttgaaaagaa aattattcgc caattaaaaa ttgaccttga caaagcagta tgatttttgt
     4321 ctgaacccga accaataaca tatttcgtat taaaaacgag agcaacaggc aattttgtcc
     4381 tgctgctctc gttttttata aaacagtatc tgagggattg gtgacagtat cagaatcatt
     4441 attatctccg tcacccataa tatcgtcaat tccgtcttca atatcatcca tcatatccga
     4501 acccgaattt cctgtttcat tttcttcgtt accgatgaaa ccgtccgaat ctgaaacttc
     4561 tccgttttca gtcatacttt ttgaagtttc ctgcgtattt gatgtttgtg aagtctgact
     4621 tgttgtatca gtcatactgt cgtttttatc attgccgcaa cctgtgataa gacatactga
     4681 aattaaagct acggcaaaca gacctaaaaa ttttttcaaa ctaatcaatc cttataaaaa
     4741 taaaatggta ctgaatcaac agcaccatta ttatttacgg aatttttcgt aatatttaat
     4801 ttggaattta ataattttaa tctttggcaa taacaagatg agggtacgga atcgtgatac
     4861 cgtttttatc aaactctttc ttgacatttt cttgcaggaa gaaataaacc tcgtagtaat
     4921 catcaggagt tgcccatgca aaaaccttaa tctgcactcc gctgtcaaga tgttcaccga
     4981 ctcttacagt aggttcggga tcttttaaaa tcatctcatt tgttgccata aggttgagaa
     5041 taatctcacg caccttgtcg atatcatcat catagcttac cgagaaagta cagtcaaccc
     5101 ttctctgctc aaccattgtg cagtttacaa cattgtttga tgtaagtctt gaattaggaa
     5161 ttgtcacaag cttattatca agtgtatgca cggttgttga gaaaatattg atttcctcaa
     5221 ccgttccttt tactccctca aaatcaagaa tattacccga cacaaacggt ttgtttatca
     5281 aaattacaag tccgctgaca acatttttca gcaaatcctg taatgcaaga cctgccgtaa
     5341 gggctgccgc accgagtgcc gtaataagcg acgcaacatt cacaccgatt gtcccgagtg
     5401 cagttactgc aacaatgaaa tagaaaatca ccttgagaac ggacaataca aagccttcgg
     5461 ctgtaccgtc cattcgtgat ttattaagga tttttcggag aatccctata atcaacctgg
     5521 taattatata tccgataaca attacagcta cacacacaag agcctttgaa agatattcaa
     5581 gcaacttttc ttcagtgata ttcatatcta cacctctgca tcaacaagaa attcagaaat
     5641 cttctctccg ttctcataaa cagaaaccac tatattgccg ttttcatcaa aagaaacgct
     5701 cttacctgtg cgaacaccgt tttcgtaatt gctcacatca ataagatttc cgtttctgtc
     5761 aaatcttgca ccatagccgt caggagtatt attatcccac ttgcccgcat gcattgtacc
     5821 gtcactcagc ctgtaaccga caccgccgcc gttgcgtgaa ttatcggtcc agtttccaac
     5881 ataatttata ctgccgttgt tataatagca aacaccgaat ccgttgcgcc tgtcatcaag
     5941 gtattcaccg tcatatgagg tcataccgtc aggagaaacc gttctgcctc tgcctgttct
     6001 gcaattattt tcatcaagat taccgaaata ggtatatctt cccgatttag taagaatcac
     6061 cacatcacaa tggggttctt cttcgtttac aatttcagga ttttcttcat cggattctcc
     6121 tgtcggcaca tcatctattt ggcaatgtgt gccgatattt gcttcaacat tttcaaaatc
     6181 agtttcttca ttttcggaat cttcgtcgga gctgtcaaca gttacaacat tttcggaatc
     6241 ttctgtctgt tcatctgctg tttctgcgac agcgtctgta tcatctgcaa tttcatgttc
     6301 tgaaatttga gaattttctt caacctcatc atccgaaagc tgttcgtctt tctcgcctgt
     6361 tacaatttta acttccgctg gttcaaaatc ttcttcggca gtatgattta taatttcacc
     6421 gaacaaatcc ttatttgttt tgtgagcctc aacaagaatt ttctcgataa tatcgtatgc
     6481 cgaataatct ttcttactca cgggttcagc cgtgtcggaa acatttggaa tatcttcgtc
     6541 cgtaagataa gcgcaatctt caagattata agcattttcg ggaacaaaat catttttgaa
     6601 caaatcaccg ttaaacacaa atccgctgtt gccgtaggat tttttctccg taaacggcat
     6661 atctgacggt aaaaaacttg catcatacca gagcataccc acatttgaat atatcaaaac
     6721 ccttgaattt tcgtttgact gcttatcaga cgggaaaaga gtttttacgg aaacaatccc
     6781 gttatcgtca atatcttcgg tgacaagagt tataattccg ctgactttgc gtggatatat
     6841 tctcgtgata agcttatcgg caatgcttct gttaaaatat tccgtgcgta accatgtatg
     6901 attacggtca aaaaattgtc ttttgaatac aattcccctg tcattgtaag ttataacaga
     6961 ataaactccg cctgttgccc tttttacgga ctgataagga agattatccc ttgttacctt
     7021 ccacgaaagc ggttcattct tcgtcagcag acaagacatc ttgtaatcca taccgtgaag
     7081 ttcgatttcg gtaatttctt tttgctcttt gcagcctgaa ttaaaatagt ttgcacggac
     7141 acccgcaagt gcatcattct taaaatcaag tttttcatag tctgaaacaa gaacggaata
     7201 tataattaag ccgtcagtca gcttttgaga ttgttcaagc attatgaatt attcctttca
     7261 agaaaattct gtgtagcgta accctaaaag aaaataaagg gcgaacaatg tccgccctga
     7321 aataatcgag ttatatttaa atcagataga aattgtcata gcaatcttgt aaagctcttc
     7381 atcaaacaca cgaggcatat caagtgcttc gttgatatca agatcaacaa tcttgccgcc
     7441 ctgcattgca acaactctgt taccgatatt ctgctcaaga agttcaacag ccttaaagcc
     7501 catctggctt gcaacaactc tgtcacgaag tgtcggagaa ccgccacgct gtacatgacc
     7561 gaggactgta gctcttgcct cgataccaag acgctgttct acatactttg caagatctct
     7621 tacaccgccg acaccctcgg caacaacaac gataaagtgt tttttgcctg ttttctgagt
     7681 gttgatgatt cttgcgatta catctctttc aatattgtac tgaacctcag gaacaaggat
     7741 agctgtagca cctgttgcga tacctgtctg gagagcaata tcaccgcagc gtctgcccat
     7801 aacctctacg attgaacaac ggtcatgtnn nnnnnnnntc aaccatctga atagctgtgt
     7861 tcatagctgt atcaaaacca accgtgtatt ccgaacaggc aatatcgtta tcaattgtgc
     7921 cgggaacacc aatacagtta atacctgcgt ttgtaaggca acgagcacct ctgaatgaac
     7981 cgtcaccgcc gattacaaca acaccgctta caccgatgct tctgcaaaaa tcggcagctc
     8041 tctgaatacc ggcaggagta gcgtactctt cgcttcttgc tgtgtaaagc attgtaccgc
     8101 cgttaccgat gatatcagat acacttctga ggttcatttc ctcaacatca ccgttgagca
     8161 aaccattata tccacggtaa acaccgtaaa ccttcattcc tttatttata ccggcacgaa
     8221 ctacagctct gacagccgca ttcataccgg gagcatcgcc gccgcttgtt aatactgcaa
     8281 ttgatttctg tgtctgtgac attataatct acctccatag gttattaact tctaccatta
     8341 taatacaatt gttttttgtt tacaagtgga ttttcgcaat tttaaaaaaa taaaagattt
     8401 tattacaaaa gcatttaatc ctttttaccg ttttgttaca gaaatctgtg aatttgttca
     8461 taaatacact gttttcacct ctctgtttcg ataaaataac aatattttgt aacaaagcat
     8521 gtaagtttgt tgacttttaa attatgaggt tttataatta tccttggata agagacagat
     8581 tttgtgcctg tcttgcgaca agtcacctga gtgacaattt ttcgggagga taatttatat
     8641 gactaagtta tatctggcta ttccctgtta taacgaagaa gaagttctga gggattcggc
     8701 ggaaaaactg ctaaacaaat acagcacgat gatggcagag ggcaaaattt ctgccgacag
     8761 ccgtattgtg tttattgatg acggctcaaa ggacaggaca tgggagatta tttcagaact
     8821 ccatacagaa aatcctattt ttcagggcat caagctttca agaaacagag gtcaccagaa
     8881 tgcacttctc tgcggtctta tgactctcaa agataaggct gacgctgtga tttcaattga
     8941 cgcagacctt caggacgata tcaacacctt tgatgaaatg gtggcaaagt acgaggacgg
     9001 ttgcgatgtt gtatacggtg ttcgttctaa aagagaaaca gacacattct tcaagagatt
     9061 tacagcagaa ggattctaca aaattcttaa tcatatgggc gctaaggtta tttttaacca
     9121 cgctgatttc agacttatga gcaaaagagc gctcaacgct ttttcattat ataaggaaac
     9181 aaacatattc ctgcgtggta tggttccgat gatcggcttt aagtctgata ttgttacata
     9241 tgagcgttcc gaaagacttg caggtgaaag taaatatccg ttaaagaaaa tgcttgcact
     9301 tgcgtgggag ggtattacat ctctcagcat tcagccaatc agaatgatta catggctcgg
     9361 tgcaatcgtg ttcggcatca gccttattat gattatctac tcactcatca gctttttctg
     9421 cggttggact gtaagcggtt ggtcaagcct tttatgttca atttgggcaa tcggaggact
     9481 tcagctcctt gcaatcggtg ttataggcga atacatcgga aagatttata tggaaacaaa
     9541 gcgcagacca agatttattg ttgacaaagt tcttgatgac gatgaacagt aatagaatca
     9601 ccaaagtcct gaccggaatt ttcggcggag tatttacggt ttgttttgtt tttctgtttg
     9661 taaatattat cataatcggc tttgacggaa atgttgacag aagattcgat tcattttcaa
     9721 agatgttcat tcttttgatt tttgccgttt tgaccgtcgg aatatgtgca ttgtattttc
     9781 attacacctc cgataaaagc tgtaagaaga ttaaagcgaa gtcgagattt gaatttaatg
     9841 ataaaaatac caaaaatgtt atttttatcg gatgcggtat tcttctgatt gttgaaatta
     9901 tttttgcgtt gcttaccgat tttgaacctg ttgccgattt gcataacatc aaaagatatg
     9961 caatgtattt ttcaacccac ggcaatttca acctcattga acaggattac gcaaacgatt
    10021 atcagtatct tgtaagatat ccgaataata tggcattgct tttgattgtg tcgcttgtcg
    10081 gaagattaaa ttaccttata ttcggtcatt atgttgattt tgcacctgtt gttgtaaaca
    10141 tttttgcgat taatatttca ataatgctga cggcatttac agcaaaaaga cttttcggca
    10201 acaaaaaggc tctttttgta cttgcttttt gtgcattgtt tttaccttat ctcacctatc
    10261 tgccgtacta ttacagtgat tcaatgtcga tgccgtttct tatcggagct gtttatctca
    10321 ttgtttccgc attacaggtt gacaacagaa agagtatgta cgcaaagctt tgtgctgcag
    10381 gtgcattgat tttcctcgga tataaggtta agggcagtct tatcatactg ttcgcagtcg
    10441 gacttttatt gttgttcttg aaattcagat taaaaaaagc aatctgcctt attctggtgt
    10501 ttacggcagg gtttggtgtg attggattcg catacaatac agccgttgac gctgtcaatc
    10561 cgatcacgaa acagcagtat gaaaaatatg aatatcctgt tactcactgg cttatgatgg
    10621 ggcttaaagg tcttggaaaa tacgatgagc atgatgacta ttacactcgt tcatttccct
    10681 cgaaaaaaga aaaacaggat gctaacatta aggttattaa agaaagaata aaagattaca
    10741 aatttagcgg tttgtatgaa catatgattg aaaaagctgt ttggacatgg caggacggta
    10801 catattacat ttcgtaccat aacagaaagc caaagaatga taatattctt atggattttc
    10861 tgcatattga cggaaagtac aataaggctt tccaaaatta cagttcggct ttacaactgt
    10921 tcattttatt gatgatttgt atttcggcag taaaaactct tatcaaacct aaagttgatg
    10981 agatgctgtt aatcaaagga gttgtatttg cggcattcct tttcttcctg ttatgggaaa
    11041 caaggtcaag atacctgttt gatatgacgc ctctgtttat tctgcttacg gttgacggta
    11101 tggataccgc aaaagctttt cttgattcat tcagaaagcc taaacaacaa acagaaaaac
    11161 aggcataatt taataaagtt ttcaagtgac agccgaaact gtcacttgtt ttttgtcata
    11221 aaatcaatgt tacaacatta caaacttttt cattaaaaat caatattttc gtttgcaatt
    11281 tattgtaatg atgcctaaat tatgctatta ttactatatg tgcaatgatt tgcaaatttc
    11341 aagcttgaaa ggtaaaaatt atggattatg aaaagctgaa aaagagggat acctcgctgg
    11401 acattttgcg aataatcgca gtctttaccg ttttatcggt tcattttttc ctacacaacg
    11461 gattttacag ccagacaatc gaagggacac cgatgtatgt tgctgttgta atgagaaccc
    11521 tgttttccgt ttgcgttccc ttgttcatgt tgctgacagg ttatttgatg agtaaaaaag
    11581 aactgagtaa aaagtactat tcgggaatta cgaaaacact tgttgttttt gtaatctcaa
    11641 cacttgcgtg tatgatttac aaaaacattg cacaaggtga tgtttttgac ctgaaatctt
    11701 tcatactcgg aacgcttgat tttaccggtt ccaactactc ctggtacatt gaaatgtaca
    11761 tagggttgtt tctgcttgca ccgttcctta atcttgctta cggaaaactt aaaagcaaaa
    11821 agcaaaagca agtactcctt gtaacagctg tttttctgac aatcatcccc tcgttattta
    11881 atattttcaa ttttggttca cttgattggt ggactaatcc gacttcatct gatgagtttc
    11941 agaaacttgt cccaagctgg tggcagggat tctaccccgt tgcatactac tttgtgggtt
    12001 gttacataag agaatacgga ttgaaaatga aaacaagaac aatgcttgta ctctttgttt
    12061 tttcactttt catattcagc acattcaatt atttcagaag ctacggcaca acattcaaat
    12121 cgggtacata catttattgg tacggatttg aaccgtttgt tttatctgta ctgctttttc
    12181 tcttaattaa gagaatcaag accgataact tcccgaaagc cgctaaaatt gcactttgga
    12241 aagtttctga ccttgcactt ggaatttatc ttatttcctt tatttttgac agcattgttt
    12301 accccgtact ctgcgaaaaa gtaatattaa tgcctgacag acttccgtat tactttgtta
    12361 cggttccgat agtttttgtg ctttccgccg ccgcatcatt tattatgaat cttgttgcaa
    12421 aactgcttat tgacggtttt aaatcgcttg cgaacataat aaaggatttg cgttcaaagc
    12481 ctgataaagg aaagtggcag cacataattt ttgctgtgct tatggctttt gctattggat
    12541 tttcactttg gaaatgctat tacggttttg gcggtaacga cgaatcgttt tatcttacaa
    12601 ttccgcaccg attcactctc ggtgattcgt tactcggtga cgaatggcac ctcacacagc
    12661 tttcaggatt tctacttttg ccgttcgtat ggctctacac aatgattacg caatctactg
    12721 tcggaataat ctttgcggca agagttttct atgtaatctg tcacgctgtt attgtgtgcg
    12781 ttatctattc aagacttaaa aagtacggat atttctcagt attcggttgt gtgctttatt
    12841 tcctctttac accgtttgac attatggcat tgagttataa cacaatggga cttgacctaa
    12901 ttgcgctcac gggtgtttta atggcaacag ccgattattc aaaaaaatta ccgctcataa
    12961 tcagcggact tgcatttgca ggcgcagttc tctgctgtcc gtaccttgcg gcagtttatg
    13021 taatgtatat tattgcaata ggagttcact atgtaattaa gaaaactgcc cttaataaaa
    13081 atgtgttcaa ttnnnnnnnn nnnnnnnnnn nnggtttacc ttgggagtag gaatacttgt
    13141 ggctattttc cttgtatttg tactttcaag agtaagtatt aatgagattt tctcaaatct
    13201 cccctatctt cttgccgatc ccgaccatcc gcaaatgggc tttatggcaa agatgaatta
    13261 ctactttaaa acaatcgtag aatgtcacac tcattttaaa tatgtactta tggcatacgg
    13321 tgctaccgca attgttatgt tgcttgacag aaagagaaaa caacaccgtt cgatttatct
    13381 tattcttaca agtgcaatcg ttattttgtc gcttgtaatg tttatgccga caatgacaag
    13441 cgtttactac aatgcgatta tgttcccgat gattttcatg ggcatcacag cgtatgttct
    13501 ttctgaaaat aagcaaagag aactgtttgc ttcactcttt gttctcggaa ttttttattc
    13561 tgttgcattg tgtttctctt caaatcagta tttttatgta actgcaatgg cttgcaccgc
    13621 atcaaatatt gcaagctttg tattcatcgg caatctgatt aaggaaatga aagcaaatcc
    13681 cgataatctt gactacgctg ttccgtgtaa atatttagca ttcgtgatga ctgcgtttct
    13741 gattattctg caggcttgtt ttcagattac cgtcaaggca gagcattgct tctgggacag
    13801 tgaaccgaaa cagctgacac agacaattca gaacggtcct gcaaagggta taaaaacaac
    13861 accgaataac gcacaaacct atgagcagat ttatgccgat atcagtcagt atcagaatct
    13921 tgaaaaagga aacatactgt tccttacaca aaaaacatgg acttatcttg cggcggagga
    13981 ttttccttac ggaacacttt cggcatatgt tacgggtgaa aatcagaaca gtcttgcaag
    14041 acttcgctca tattattcgg taaacagcaa aaaaattccg aaatatatct acattccgaa
    14101 ggactcagag tgggataatc ttcagcagat tcttcttgag gcacagcaga acggttactc
    14161 tttgagcgaa aacgaagttt cgtataaact tgtaaaataa ataatatttt acagatttca
    14221 gacataaata tcacgcaggg ataaaaatta tgatttctgc gtgatatttt tttgtttttc
    14281 agcacatatt cttgaaaatg tacaaataaa aaacttgttt ttttttgtga attatggtat
    14341 agtgaattat gataggcaag aagggcttgt cacagaattt ggtcgcattt ttgtgaaaat
    14401 tttaagttgg gggcataatc atggatatta agcagactta taacgaatgg cttgaaaacg
    14461 cagttgagga caaagacctt acggcagagc ttgaaagtat aaaaaacaac gaagatgaaa
    14521 tttatgatcg tttctacaca gctttaaaat tcggtaccgc aggcctcaga ggcattatcg
    14581 gtgcaggtac taacagaatg aatatttatg ttgttcgtca ggctacacag ggacttgcta
    14641 actatgtgct taaaaagtac ggcaatggtt ctgttgcaat ctctcacgac agcagaatta
    14701 aagcagattt atttatgaat gaggcggcaa gagttcttgc ggcaaacggc attaaggttt
    14761 acatcacatc agaacttcag cctactcccg ttctctctta cctcgttcgt tactttaaat
    14821 gtcaggcagg tattatggtt acagccagcc acaaccctgc agcttacaac ggctataagg
    14881 catacggtga gtccgaagaa ggcgctgatc agcgcgtggc g
//
LOCUS       DAOMVE010000031        14813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_858_length_14813_cov_3.237295, whole genome shotgun sequence.
ACCESSION   DAOMVE010000031 DAOMVE010000000
VERSION     DAOMVE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14813
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_858_length_14813_cov_3.237295"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..463)
                     /locus_tag="PLS64_03010"
     CDS             complement(236..463)
                     /locus_tag="PLS64_03010"
                     /inference="COORDINATES: protein motif:HMM:NF013753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRL42835.1"
                     /translation="MRFLLVRFAVQKEKQLPGLTAHAIHPISTGKLEGFNNKIKVAKR
                     IGYGYRDDDFFFTLIRYLSIPSVRSPSHKKS"
     gene            complement(594..1385)
                     /locus_tag="PLS64_03015"
     CDS             complement(594..1385)
                     /locus_tag="PLS64_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42836.1"
                     /translation="MKYKILSAIISIVLMISLVGCQQNNTIIKPDIETKTVTENSEGD
                     YITFSLDIPLNWKLYAQNYTSIACFSPDANETNAGEKISPYKLSIDNYIIPSLMPIDD
                     KYKQAYKDLFNGKYDGVKELICDNIEYINIGKIMDSFPDVNFETPESIMDYFNILVDG
                     TSTPDVTMPESWNDKKWATDFTFSEYNGKNGKIIAVEYSYVIIDKTYKAINCYRDDDF
                     SVCGAFDDDTDLSSGDLALWVADNMEITKHYKIEDNMIKQEGVDY"
     gene            1596..1853
                     /locus_tag="PLS64_03020"
     CDS             1596..1853
                     /locus_tag="PLS64_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42837.1"
                     /translation="MTDQILIALISFAGTLAGSLAGIFTASRLSNYRIEQLEKKVDKH
                     NNLVERVYLIEQHEAVNDNKIKTAEHRIADLENEQNKERKI"
     gene            1858..2094
                     /locus_tag="PLS64_03025"
     CDS             1858..2094
                     /locus_tag="PLS64_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42838.1"
                     /translation="MSKIKICLKKPCLKRAFRTFIQTALGYVITNVTLALGGLDFNDG
                     DVVKNALIGLAVAAVAAGASAVMNLKEGEDKDNE"
     gene            2087..2866
                     /locus_tag="PLS64_03030"
     CDS             2087..2866
                     /locus_tag="PLS64_03030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRL42839.1"
                     /translation="MSKKIYLSPSNQNGNTYATGGTNEMAQCDKIAAAAAKALKRCGF
                     DVMVAKSGTLMQTRCPESDKFGADIHMPIHTNAFNGKYTGGTRVFCLNSNGRKAAEAV
                     KSALGAISPGKDDSVSYKTDLYEINVPKALTVYVECEFHDTVTGSDWIRKNTVAIGEA
                     ICKGMCNYFDVKYKTDSAGSDSSKAGSDKAFRRYIVRITSSNGVNIRKGPGTNYDVNG
                     AVPKGGAYTIVDEKSGAGAAKWGKLKSGAGWIALDYTEKIR"
     assembly_gap    3407..3416
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3417..4680)
                     /locus_tag="PLS64_03035"
                     /pseudo
     CDS             complement(3417..4680)
                     /locus_tag="PLS64_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005360577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
     gene            5043..5249
                     /locus_tag="PLS64_03040"
     CDS             5043..5249
                     /locus_tag="PLS64_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42840.1"
                     /translation="MVNYFEWSMEYKNTADSIQDVIDRLKAEKRGKSEINKKELDLKI
                     AKYKIYYNECIHISNHLMDRYYGA"
     gene            5254..5553
                     /locus_tag="PLS64_03045"
     CDS             5254..5553
                     /locus_tag="PLS64_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL42841.1"
                     /translation="MKREFIHITDNNKHRLMSDSGFWHGSTNKNSRDKMKKILSMAVL
                     NELTERQRICIVDYYLNGKKEKEIAKELGVNSSTVSRHIMKARDKLRHIASYYVN"
     gene            complement(5550..6158)
                     /locus_tag="PLS64_03050"
     CDS             complement(5550..6158)
                     /locus_tag="PLS64_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42842.1"
                     /translation="MKEKLIAVVLLLILTAILPIAVSKCSERSFAKPTVSTSDTPEKP
                     KDSGEILCALTAGLYKDSYSAETLKAIAILMNTNYKANPDSFKANDFLYEENASGSIK
                     DVYGEIKKAAESAKNKTLRKNSEALFVPYSETSNGTTYKNENYRYIHSVASPWDCYQT
                     DFDANAECVGVSLSGIDYLCKNGCSAEEALLWYLPDFEIADD"
     gene            6400..7713
                     /locus_tag="PLS64_03055"
     CDS             6400..7713
                     /locus_tag="PLS64_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42843.1"
                     /translation="MKTKYSVIPFIPAVIAAIGLKIMSMFAVDGNGLLFGMNKSGINY
                     FIIEIILGLFAVCVLINLFDRKTAPVYPVKKNFVSGAFAVISGIAIVGSSFLALINAT
                     PGNTDNYLLSIIAALFSIPAAIAMIMIGKVHFSGKSIVSNASMFFVFPALWGCSELVT
                     EFLAATKVSISASDMTPLFCYIFITLYLFSSSMVVSRVKGRNPVKACFIYGMPAAAVS
                     LSYGVGAVLTSSVENTGISALVNGVMFIVLGIYIVSFSVEMFAGCLTKAEVEIIESLP
                     EDDDTYENSYISSGGYDELVVSDKKEDDVPASDDSYEAVAQGLSDFVMGYDEEKYDDA
                     TIKNENINDGLVLGYDNENQTQPAEKIATEPLKEETAKPVAEAPAEPEIPEEPVYAKR
                     LDVSEEKTEPVKAEAPVKAPVEDTEVSDDRMSEIDRLLKELEDKK"
     gene            8039..9985
                     /gene="thrS"
                     /locus_tag="PLS64_03060"
     CDS             8039..9985
                     /gene="thrS"
                     /locus_tag="PLS64_03060"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRL42844.1"
                     /translation="MINVELKGGAVKEFENGTTPAEIAKSIGAGLYKSVCCAKVDGDL
                     CDLRTPLEKDCKVELLTFDNVDGQKTFWHTASHVLAQAVKRLYPNAKCAIGPAVDNGF
                     YYDFDVEKPFSPEDLEKIKAEMKKIVKSGLELERVELSPEEAEKKLEEMNEPYKVELV
                     KEHSDKGEHITFYKQGEFIDLCAGPHLMSVAPIKAIELTACTGAYWRGDANNAQLCRV
                     YGVAFPKASMLEEHLKKLEEAKLRDHNKLGRELEYFTTVDYVGQGLPILLPKGARVVQ
                     LLQRWVEDVEQSKGCLLTKTPLLAKRDLYKISGHWDHYLDGMFVLGDPHDEEKECFAL
                     RPMTCPFQYQVYLNKQRSYRDLPMRLTETSTLFRNEASGEMHGLIRVRQFTISEGHYI
                     LRPDQLEQEFKGCLELAKYFLDTVGLLENCTFRFSQWDPENKNNKYEGTKEQWEESQA
                     VMKTILDDLDVDYEIGIDEAAFYGPKLDIQYKNVFGKEDTIVTIQIDMLLAERFGMYY
                     IDKDGQKKLPYIIHRTSLGCFERTLAYMIERFAGVMPLWLAPEQIRLLPIKEGNVEYA
                     QGIADRLTSLGMRVTVDSRDENIGPKIKAARLERIPYILVIGDNEMNSSTVTVRSRKR
                     GEIPNMSVDEFVALVKNEVDTKEK"
     gene            10013..10807
                     /locus_tag="PLS64_03065"
     CDS             10013..10807
                     /locus_tag="PLS64_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRL42845.1"
                     /translation="MSGGFTPFDKNSYRESKRRRKQALIEERTGRILPVSNPRRRYIA
                     LACIGVILVAAIAFGIFSILKSNQKSNPETPEQIQAESEMLLTVVDSANPLDKDYMPD
                     LETVNGFRVNRDALKPLENLLKASKENGTELKIKTAYVSYDEQEERFQSELQKMLQSS
                     KYTTVRAEAEVLKTTPHGGQSESQTGLLVEFDFSNSGSKAFLERNCVEYGFVQRYTES
                     KTSVTRMNPSDSLYRYVGIENAKRMRSYGMCLEEYKSYLQKQAIPK"
     gene            10897..11100
                     /gene="rpmE"
                     /locus_tag="PLS64_03070"
     CDS             10897..11100
                     /gene="rpmE"
                     /locus_tag="PLS64_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRL42846.1"
                     /translation="MKENIHPKYEKTTITCACGNVIETGSTKKDIRVEICSKCHPFFT
                     GKQKLVDTGGRVDRFKKRFGMDK"
     gene            11203..11832
                     /locus_tag="PLS64_03075"
     CDS             11203..11832
                     /locus_tag="PLS64_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HRL42847.1"
                     /translation="MAKDYKTVLSNAKDEFVEKRSRFIGYCKPVTCEQEAVDFINEKR
                     SEHWNATHNVYAYSLREGNIKRYSDDGEPSGTAGMPCLDVIVKNEIYDVCVVVTRYFG
                     GVLLGTGGLVRAYSHGAKIALDAAKIVMMQNCLVCSARCTYNRYGKVSALVTDNGGAV
                     DDTVYEGDVLIKFHIKPDLLPQLNKKLADATAGEVQAVFDEEKYYAVEL"
     gene            11976..13742
                     /gene="dnaG"
                     /locus_tag="PLS64_03080"
     CDS             11976..13742
                     /gene="dnaG"
                     /locus_tag="PLS64_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRL42848.1"
                     /translation="MAFPESFLQDLKMRNDITDVVSGYVNLKRRGRNMVGLCPFHGEK
                     TPSFNVYTENGSFYCFGCGVGGDVISFIMKIENLDYVDAVKYLAQRAGLEMPENSYDD
                     SMSRLRNRVFEANREAARFYYAALCSPQGRKGLDYFHSRALSDRTIRHFGLGYADDNW
                     SSLCNHLKSKGFNDSELVAANLAVRHRNGNGIYDRFTDRVMFPIIDLRGNVIAFGGRI
                     MSDAKPKYLNTSDTPVFKKSANLFSLNNAKNSGKRTLILCEGYMDVIAVNQAGFTNAV
                     ATLGTALTGEQAVLMKRYADEVIICYDADEAGQKATARAIPILRNSGLNVKVLTIPSG
                     KDPDEFIKSKGNDGPAAFKALLDKCGNDVEYRLQKLKNAHNIQLTEGKVAFLEEAAKL
                     IATISSPIERDVYSSKVASELGVDKNAFKQQVSRVSRRGERAEEKKQARQIQLELSRR
                     NDKINPEHFQKPRSSSAEEALLVYLLNNPDAYEEISARVKPEQFQNTLMRRFFEYFSA
                     RIERGEDPLTNTAADFTQDENSKLYQLMSQAIPGASTAEAMNEYINVINEESSKLNSG
                     DLADVSNEELMAYLDKIRKKKQ"
     gene            13764..>14813
                     /gene="rpoD"
                     /locus_tag="PLS64_03085"
     CDS             13764..>14813
                     /gene="rpoD"
                     /locus_tag="PLS64_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRL42849.1"
                     /translation="MAATPDKKTVVRELMELGKQKGQLTNQDILDAIGEIDFDPEQLE
                     KLYDSLAQLGIEIVEDSGDIKIDDIDLGFDEGKDGDSHAQIAETGSVSVDDPVKVYLK
                     EIGRVPLLSSDEEINLAIRIADGDVAAKQRLSEANLRLVVSIAKRYLGRGMQFLDLIQ
                     EGNLGLIKAVEKFDYTKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKVK
                     KVQSQLLHQNGHEPSADEIAAEIDMPVDKVREIMRVAQEPVSLETPIGEEEDSHLGDF
                     IPDNDAPAPADAASHTMLREQLSDVLSTLTPREAKVLKLRFGLEDGRSRTLEEVGKEF
                     NVTRERIRQIEAKALR"
BASE COUNT         4600 a         2789 c         3186 g         4228 t
ORIGIN      
        1 catatttgcc cgagcgttac ttttagattg taaataaaaa cgagaaataa aaaaacggtg
       61 tgcaacgaag cggcacaccg aaaaatgatt gacatattaa tgttagatgt taatatatag
      121 ataaggaaac ggctttagct gttccgcttt taagacaaat tatttttggt aaccgccttg
      181 tattcgcagt acagggcggt tatttcttta tggtaaacac aataaaaaaa gttcatcacg
      241 attttttgtg ggatggtgaa cggacagaag gaatagaaag atagcgaatc aaggtaaaga
      301 agaagtcatc atcacggtaa ccgtagccga ttcgtttagc gaccttgatc ttgttgttga
      361 agccttcgag cttgccggtg ctgattgggt ggatggcatg ggcagttaag ccggggagct
      421 gtttttcttt ttgaactgca aaccgtacca ataaaaatct cagtttatag ggggcgaata
      481 tctattttga atttagcgag agcttgtaaa atggcaccca ataaaatact ggagccgaag
      541 tagaaaaata cttcggctgt aattataata tatagatttt aaaaacataa aatttaataa
      601 tctactccct cttgctttat catattatct tcaattttat agtgtttggt aatttccata
      661 ttatcagcaa cccacagtgc taaatcgccc gaagaaagat ctgtatcatc gtcaaaagct
      721 ccgcatacgg aaaagtcgtc atcacggtag caattaatag ctttgtaggt tttatctata
      781 attacatagg aatattctac cgcaataatc tttccgttct taccgttata ttcactgaaa
      841 gtaaaatcag ttgcccattt tttgtcattc catgactccg gcattgtaac atccggtgta
      901 cttgtaccgt caacaagtat attaaagtaa tccataatgc tttcaggagt ttcaaaatta
      961 acatccggga aactgtccat tatcttacct atatttatat attcaatgtt atcgcaaatt
     1021 aattctttaa caccatcata tttgccatta aataaatctt tataagcttg tttatatttg
     1081 tcatctattg gcattagtga tggaatgata tagttatcta tgcttaactt gtagggagaa
     1141 attttttcgc cggcatttgt ttcgtttgca tcaggactaa aacaagcaat agatgtataa
     1201 ttctgagcgt ataatttcca gtttaaagga atatcaagtg aaaaagttat ataatcacct
     1261 tcgctgtttt ccgtcacagt ttttgtttct atatcaggct taataattgt attgttctgc
     1321 tgacagccta caagcgaaat cataagtacg atggatatta ttgctgataa gattttgtat
     1381 ttcatttgta tgctcctatt atatatttca atttaaaatc acttgagatt tttgatttta
     1441 ttcatttaat attactaaat tgtgatatct attgtactgt ttgtattgct ttttgtaaag
     1501 taataataaa aataaatata aagaaatttc ggagaaattg cattgtttag tgcaatttct
     1561 cctttttttg agcctgtaag tgggaggtga tttttttgac agaccaaatt cttatagcgt
     1621 tgatttcatt tgcaggaact cttgccggct cacttgcagg catttttaca gcgtcaagat
     1681 tatcaaatta tcgcattgaa cagctcgaaa aaaaggttga caagcataat aatcttgttg
     1741 agcgtgtata tcttattgag cagcacgagg cagttaatga caacaaaatc aaaacagcgg
     1801 aacacagaat agcggatttg gaaaacgaac aaaacaagga aaggaaaata taatataatg
     1861 agtaaaataa aaatatgctt aaaaaaaccg tgtttaaaaa gagcgttcag aacatttatt
     1921 cagactgctc tcggttatgt tatcaccaat gtaacgctcg ctttgggcgg tcttgatttt
     1981 aacgacggtg atgttgtaaa gaacgctctc atcggtcttg ctgttgcggc tgttgcggca
     2041 ggtgcatcgg cagtaatgaa tttaaaagag ggagaagata aagacaatga gtaaaaaaat
     2101 atatctcagt ccgtcaaacc aaaacggcaa cacctatgca acgggcggta cgaatgaaat
     2161 ggctcagtgc gacaaaattg ccgccgcagc agccaaagca ctcaagcgtt gcggattcga
     2221 tgttatggtc gcaaagtcgg gcacgcttat gcagacacgc tgtcccgagt cggataagtt
     2281 tggagcagac attcatatgc cgatccacac taatgcgttt aacggcaaat atacgggcgg
     2341 tacaagagtg ttttgcctga actcaaacgg cagaaaggct gccgaggcgg taaagtctgc
     2401 cctcggagca atctcgcccg gcaaggacga ttcagtcagc tacaaaaccg acctctacga
     2461 aatcaatgtg ccgaaggcat taaccgtgta tgttgaatgc gagtttcacg acaccgtgac
     2521 aggctcggat tggatacgca agaatacagt tgcaatcgga gaggcaatct gcaagggtat
     2581 gtgcaattat tttgatgtga aatacaaaac tgactctgca ggctcagaca gttcaaaagc
     2641 gggttcggat aaagctttca ggcgttatat cgtgagaata acctcgtcaa acggcgtgaa
     2701 tatccgtaaa ggtccgggta caaattacga tgtgaacggt gctgttccaa agggcggagc
     2761 gtacacgata gtcgatgaaa agtcgggtgc aggtgcagcc aagtggggca aactcaaaag
     2821 cggtgccggc tggatagcac ttgattatac tgaaaaaata agataaagca aaaaaggagt
     2881 cgtgattttt cacggctccg atttttatgc agaaattatg taattatgaa ttttttccaa
     2941 acgcaaaaaa gaccgtcaaa tgacgatcct tttcgcgatt gtgtgtttta tgaaaatgaa
     3001 tgtttcaata aagaagcaaa catttttgcc gaatatttaa tgaacaggta agattaaagt
     3061 tttgaacttt atctttattc attgtcttat attataccga tttttagggg taaattcaag
     3121 gtgagagggt gcaattctaa tattcataca gtcgaaaaga caaactgtac aaaaaatcaa
     3181 gaagatgttt gtgcagtatt tttttagaat cttaaattac ccgacaaaaa cgatgatttt
     3241 aataattggc tctatacaaa ataaacaaaa agtaaatcaa taagaaatat tgtttttact
     3301 gttaaacacc atttttaatt ggattaatct ttttcctgcg gatatttgcc ataaaaacaa
     3361 gtgcaacgat aaaggctaca gcttcgccga tcgggaatgc aatccannnn nnnnnnaaaa
     3421 gatgtatgca aggggcagag gaacaacaat aagtcgtaca agcgaaagta tcagcgaatg
     3481 cacaccgttg ccgagtgcct gaaaaattcc ctgatacgca atgttagcac cgacaaatat
     3541 aaatccgggt gtgattattc taattgcaag ctttgcaagt tcggttgtcg attctgtaag
     3601 cgagaatatt ccgagtatct gttttgcaaa aatttgaaga attataattc ctgcacaaag
     3661 aattgatacc gtgtaaataa gtccgtattt aatgctgtcg tttactcgct ttttatcctg
     3721 tttgccgtag ttgaacgcta taatcggaat catcgcattg ttcatgccga atgcggcaaa
     3781 aaatacaaac tgctgaattt tgtagtagat accgtaagcc gtaaccgcat ccgttgaaac
     3841 tcttacaaaa ataacattaa tgctgtatgt caaaatcgac ataagcgcct gcataataat
     3901 agcggggata ccaacctgat aaatctgcct gataatcctg ccttcgggtt tcatatactt
     3961 taatgatgtg ttgaaatgct ttgaattgca tctgaaatag aaatatgcgt caataaagaa
     4021 tgaaatgaac tgacctataa ccgttgcaat agcagcaccg tcaataccga gcttcgggca
     4081 accgcaccaa ccgaaaatca taatcggatc aagtatcatg ttcacaaccg caccgacaat
     4141 ctgcgcaact gttgtctgca ttgtatatcc cgttgcctgt aacagctttt caaaaatcat
     4201 gtacatacta acgcccagtg aactgtaggt gcagatttta agatacgaaa cagccattga
     4261 tgtgataatc ggatcgcttg tttgtgaggc tatgtatgcg tcaactccga aaattccgaa
     4321 aagcacaaat acaacacagg tgcaaagtgc aagaaaaatt gagttgcccg aaattttact
     4381 gcacttttct ttattgccct caccaagact tcttgacaaa atcgagttga tgccgactcc
     4441 cgtaccgact ccgaccgcaa ccataagcat ctgaacagga aatgccagcg tgagtgcatt
     4501 taccgcataa tcaccgagtg agggtatatt cggatctttc atacagctta caaagtagct
     4561 gtcaacgata ttgtacacag cctgtacaac cattgaaatt atcatcggaa cacccatagt
     4621 aagcaaaagc ctgcttattt tttcagttcc cattttattc attttttgcg ttttttccat
     4681 tatataacct ccgatgtttt tgacacaaaa atgtgcagca ttgaaaatcc ggacttatgc
     4741 cattcaatgc tgcacgatta ctcttattat acacttttta ttatgctttt gtaattgatt
     4801 tacacaccaa aattttggta aaaacgcaaa atctttttgt tgattatccg aattatatgt
     4861 cataaacaaa gaaattttga aaaaacagag catagtccgt acaaagggac agtgaatgta
     4921 tgtcacttat aatatcgaac aaatgttctt gaatttgttc gatatgttct gtataatgga
     4981 attagaaggt tgaaagcccc gccgaccttc taaatgaata aaatcaagga aaggatatcg
     5041 gtatggttaa ttattttgaa tggtcaatgg aatataaaaa tactgccgac agcattcagg
     5101 atgttatcga cagactcaaa gcagaaaaaa gaggcaaatc ggaaatcaac aaaaaagaac
     5161 ttgatttgaa aattgcaaaa tacaagattt attataatga atgcattcac atttcaaatc
     5221 atcttatgga tagatattac ggagcatgat aaaatgaaaa gagaatttat acatattaca
     5281 gataacaaca aacacagatt aatgtcggat tcgggatttt ggcatggcag tacaaataaa
     5341 aactcccgtg ataagatgaa gaaaattctt tcaatggctg ttctgaacga gcttaccgaa
     5401 agacagagaa tctgtattgt agattactat ctcaacggca aaaaggaaaa ggagattgca
     5461 aaagagctgg gagtcaactc gtcaacggtt tcccgtcaca ttatgaaggc aagggataaa
     5521 ctcagacata ttgcgtctta ttatgtaaat taatcgtcag caatttcaaa atcgggaaga
     5581 taccacagca atgcttcttc ggcactgcag ccgtttttgc aaaggtaatc aattcccgaa
     5641 agactcactc cgacacattc ggcatttgcg tcaaaatcgg tttggtaaca atcccacgga
     5701 gatgcaacgc tgtgaatata cctgtaattt tcgtttttgt atgtagtacc gtttgaagtt
     5761 tcggaatagg gaacaaacaa cgcttcgctg tttttgcgga gcgttttgtt ttttgcactc
     5821 tcagcggctt tttttatttc accgtaaaca tcctttatac ttcccgatgc gttttcttca
     5881 tacagaaaat cgtttgcctt gaaagaatcg ggattagctt tgtaatttgt gttcataaga
     5941 attgcaatcg cctttagtgt ttcggcagag tagctgtctt tatacaatcc tgccgtcaat
     6001 gcacagagaa tttcacccga atcttttggc ttttcggggg tgtcggatgt tgaaactgtc
     6061 ggttttgcaa aagaccgttc agaacatttt gaaactgcaa tggggaggat tgccgtgaga
     6121 atcaacagaa ggacaactgc aattaatttt tccttcatta ccatgctcct ttatttcgca
     6181 caaaaaaata aataatacta ttgtattttg tgacttattt tttcttgaca taacggttat
     6241 gttggcttaa aataatatat gattgattcg aaataataat tacttacaat tcattttgta
     6301 tttgtttttg ctgtttgatt tttcggcggc atatcaataa tcgtgtaaca aagtcgtttt
     6361 ttaaaattgt cttgttacgg tacagaaacg gagattttta tgaaaaccaa atattcagtt
     6421 ataccattta ttccggctgt aatagctgct ataggtctta aaattatgag tatgttcgca
     6481 gttgacggca acggattgtt gttcggcatg aacaagtcgg gcattaacta tttcataatt
     6541 gaaatcatac tcgggctttt tgcggtttgt gttttaatta acctttttga cagaaagacg
     6601 gctcccgtat atcccgttaa aaagaatttt gtttcgggtg cgtttgcagt aatttcgggt
     6661 attgcgattg tcggttcgtc atttttggct ttgattaacg caactccggg taacacggat
     6721 aattatcttt tgagcatcat tgcggctctg ttctcaattc ctgcggctat tgctatgatt
     6781 atgatcggca aggtgcattt ttcaggcaaa tcaatcgtat caaatgcatc gatgtttttt
     6841 gttttccctg cactctgggg atgcagtgaa ctcgttacgg aatttctcgc cgcaacaaag
     6901 gtttcaatct cagcaagcga tatgacaccg ctcttctgct acattttcat cacactttac
     6961 cttttctcaa gttcaatggt tgtttcaagg gtaaagggca gaaaccctgt taaggcctgc
     7021 tttatttacg gtatgcccgc tgccgctgtt tcactttcat acggtgtcgg tgctgttttg
     7081 acatcatccg ttgaaaacac aggcatcagt gctcttgtga acggagttat gtttattgtt
     7141 ctcggcattt atattgtttc gttttctgtt gaaatgttcg caggctgcct tacaaaggcg
     7201 gaggttgaga ttatcgaaag ccttcccgaa gatgatgata catacgaaaa cagctatatc
     7261 agctcaggcg gttacgatga gcttgttgtt tccgataaaa aggaagatga tgttcctgca
     7321 agcgatgaca gctacgaggc agttgcacag ggactttccg attttgtgat gggctacgat
     7381 gaggaaaaat atgacgatgc aacaatcaaa aacgaaaata taaatgacgg tcttgttctc
     7441 ggctacgaca atgaaaatca gactcagcct gcagaaaaaa ttgccacaga gcctttaaaa
     7501 gaggaaactg caaagcctgt tgcagaagct cctgcagaac ctgaaattcc cgaagaaccc
     7561 gtgtacgcaa agcgtcttga tgtttcagag gaaaaaaccg agcctgtaaa ggcagaagct
     7621 cctgttaaag cccctgttga agacacagag gtttcagacg acagaatgag cgaaatcgac
     7681 agacttctca aggaacttga agataaaaaa taattaagaa catcgaatta ggtgttgaca
     7741 ttcaggtttt agagtggtac tatacaaatg aaaagaaaat tgatttttga tgaggacacg
     7801 atttgcagtc ctgctttttc cagagagcga aattatttgc tgtgatattt cgcaaaaagc
     7861 attgcaagtt accaccttta agactgtgca ggaaatggca cggcgtttgg ttgcgttaaa
     7921 accgtcaaga gggctttaag ccgaacttgg gtggaaccac gatttattcg tcccaatatt
     7981 ccgtttttgg aatattggga ttttttgttt tcatcacata ttaaaaagga gaatggttat
     8041 gattaatgtt gaattaaaag gcggagcagt caaggaattt gaaaacggta caactcctgc
     8101 cgaaattgca aaatcaatcg gtgcaggctt gtacaagtct gtttgctgtg caaaggttga
     8161 cggcgacctt tgtgacctca gaacaccgct tgaaaaagat tgtaaggttg aacttctcac
     8221 atttgacaat gttgacggtc agaaaacttt ctggcacaca gcctctcatg ttcttgctca
     8281 ggcagtaaaa agactttatc cgaatgcaaa gtgtgcaatc ggtcctgccg ttgacaacgg
     8341 tttttactat gattttgatg ttgaaaagcc tttctcacca gaagaccttg aaaaaatcaa
     8401 ggctgagatg aagaaaatcg taaaatcagg tcttgagctt gaaagagttg agctttctcc
     8461 cgaggaggct gaaaagaaac ttgaagagat gaacgagcct tacaaggttg aacttgttaa
     8521 ggaacattcc gacaagggcg agcatatcac attctacaaa cagggtgagt ttatcgacct
     8581 ctgcgcaggt cctcacctca tgagtgttgc accgatcaag gctattgaac tcactgcctg
     8641 cacaggcgct tactggagag gcgacgcaaa caacgcacag ctttgccgtg tttacggtgt
     8701 agctttccca aaggcgtcaa tgcttgaaga acacctcaaa aagcttgaag aagcaaagct
     8761 tcgtgaccac aacaagctcg gtcgtgaact tgaatacttt acaactgttg actatgtcgg
     8821 tcagggactt cctattcttc ttccaaaggg tgcaagagtt gttcagcttt tacagagatg
     8881 ggttgaagat gttgagcagt caaagggctg tcttcttaca aaaactcctc ttcttgcaaa
     8941 gagagatctt tataagattt caggtcactg ggatcattat cttgacggta tgtttgtact
     9001 cggcgatccg catgatgaag aaaaggaatg cttcgcactt cgtcctatga cttgcccgtt
     9061 ccagtatcag gtttatctta acaaacagcg ttcatatcgt gaccttccga tgcgtcttac
     9121 ggaaacatca acacttttcc gtaacgaggc aagcggtgag atgcacggtc ttatccgtgt
     9181 tcgtcagttc acaatttcag aaggacacta cattctccgt cccgatcagc ttgaacagga
     9241 attcaagggt tgtcttgagc ttgctaaata tttccttgac acggtaggac ttcttgaaaa
     9301 ctgtactttc agattctcac agtgggatcc cgaaaacaag aataataagt atgagggtac
     9361 aaaggaacag tgggaagagt cacaggctgt tatgaaaact attctcgatg accttgatgt
     9421 tgactatgag attggtattg acgaggccgc attctacggt ccgaagcttg atattcagta
     9481 caagaatgta ttcggtaagg aagacacaat tgttacaatc cagattgata tgctccttgc
     9541 cgaaagattc ggtatgtact acatcgacaa ggacggtcag aagaagcttc cgtatattat
     9601 tcacagaact tcactcggtt gctttgaaag aactcttgca tatatgattg agagatttgc
     9661 cggtgtaatg ccgctttggc ttgctcccga gcagatcaga cttcttccga taaaagaggg
     9721 taatgttgag tatgcacagg gtattgcaga cagacttact tcactcggca tgagagttac
     9781 tgttgacagc agagatgaaa atatcggtcc aaagattaag gctgcaagac ttgaaagaat
     9841 tccgtatatc cttgtaatcg gcgacaacga gatgaattca tcaacagtta cggttcgttc
     9901 aagaaaaaga ggagaaatcc cgaatatgtc tgttgacgaa tttgttgctc ttgttaagaa
     9961 cgaagtagat acaaaagaaa aataattctg aaattttttc ggagggatgc tgatgtccgg
    10021 aggattcact ccgtttgata aaaacagcta cagagaatca aaacgcagac gaaaacaggc
    10081 gcttattgag gagagaacgg gaagaattct tcccgtatca aatcctcgcc gaaggtacat
    10141 tgcgttagct tgtatcggtg tgattcttgt cgcagctatt gcgttcggaa ttttttcaat
    10201 acttaaaagt aatcagaaaa gtaatcccga aactcccgaa cagattcagg cagaaagtga
    10261 aatgttgctg acggttgttg acagcgcaaa tccgcttgat aaggattata tgcccgatct
    10321 tgaaaccgtg aacggattca gagtgaacag ggacgcactg aaacctcttg agaatctttt
    10381 gaaagcgtca aaagaaaacg gcaccgagct taaaatcaaa actgcatatg tgagttatga
    10441 tgaacaggaa gaaaggtttc agtccgaact tcaaaagatg ttgcaatcat caaaatatac
    10501 aactgtcaga gcagaggcgg aggtcttaaa aaccacgcct cacggaggac agtcggagtc
    10561 acagacggga ttgcttgttg aatttgattt ttcaaattcg ggttcaaagg catttcttga
    10621 gcgaaactgc gttgaatacg gatttgttca gagatatacc gaaagcaaaa cctcagtaac
    10681 acgaatgaat ccgtcagact cgctgtacag atatgtgggg attgaaaacg caaagcgtat
    10741 gcgttcctac ggaatgtgcc ttgaagagta caaaagctac cttcaaaaac aggcaatccc
    10801 aaaataaaat aaatattttt taaaaacgct tgcattttgc aatattatgt tgtataatta
    10861 atccgttaat tgtgtaatgt caggtaggtg aaaacaatga aagagaatat ccatcctaag
    10921 tatgagaaaa caacaattac ttgtgcttgc ggtaatgtta ttgaaacagg ttcaacaaag
    10981 aaggacatcc gtgttgaaat ttgttcaaaa tgccacccat tctttacagg aaagcagaaa
    11041 ttagttgata ccggcggacg cgttgacaga ttcaagaagc gtttcggtat ggataaataa
    11101 tttattatgt aattgtttag gcggcacatt gtgccgcctt gtttacttta cggaaacttt
    11161 gcataaccgt gtgcagagtg tctgtataat tgaggtatta ttatggcaaa ggattacaaa
    11221 accgttttaa gtaacgcaaa agacgaattt gttgaaaagc gttcacgctt tatcggctat
    11281 tgcaagcctg taacctgtga gcaggaggct gttgatttta tcaacgaaaa acgcagtgaa
    11341 cattggaacg caactcacaa tgtctatgcc tattctctgc gtgagggcaa cataaagcgt
    11401 tacagcgatg acggtgaacc ctcgggtaca gccggtatgc cttgccttga tgtaattgta
    11461 aaaaatgaaa tttatgatgt atgtgttgtt gttacccgct acttcggcgg tgttttgctt
    11521 ggcactggcg gacttgtgag agcatattct cacggtgcaa aaattgccct tgatgccgca
    11581 aaaatcgtta tgatgcagaa ctgtcttgtc tgttctgctc gttgtacata caaccggtac
    11641 ggcaaggttt cggcacttgt aactgataac ggcggtgctg ttgacgatac tgtctatgaa
    11701 ggcgatgtgc tgattaaatt tcatattaaa cccgatttac tgccacagct taacaaaaag
    11761 cttgcagatg caactgcggg tgaggttcag gcggtttttg atgaggaaaa atattatgcc
    11821 gttgagttat aaagttttct ttatgacaaa actttttatg ctttcgacct caaaagctaa
    11881 aaaatgaaat acttttcaaa ataatttcaa atatttaaag gaatattcaa aaaaatttcg
    11941 taaaaagcta ataggtaagc gagaggagga gttttatggc tttccctgaa agtttcttgc
    12001 aggatttaaa aatgcgaaac gatatcacgg atgttgtttc gggttatgtc aatctcaaaa
    12061 gacggggaag aaatatggtc ggtctttgtc cgttccacgg cgaaaaaact ccctctttta
    12121 atgtatatac cgaaaacggt tcgttctatt gttttggctg cggagtaggc ggagatgtca
    12181 tctcttttat tatgaaaatt gaaaatcttg actatgtcga tgccgtgaag tatcttgcac
    12241 agcgggcagg acttgaaatg cccgaaaaca gctatgatga cagcatgagc cgtcttagaa
    12301 acagagtgtt tgaagctaac cgtgaggcgg caaggtttta ttatgccgct ttgtgttctc
    12361 cgcagggcag aaaaggtctt gattattttc attcaagagc cttatccgac agaacaatcc
    12421 gtcatttcgg actcggttat gccgatgata actggtcgtc actatgcaat catctcaaat
    12481 caaaaggctt taatgacagc gaacttgttg ccgcaaatct tgcggttcgg cacagaaacg
    12541 gcaacggaat ctatgacaga ttcacagacc gtgttatgtt tccgattatt gatttgagag
    12601 gaaatgttat tgcattcggc ggaagaatta tgtccgatgc aaagcctaaa tatctcaaca
    12661 cctccgatac ccccgtattt aaaaagagtg caaacctgtt ttcactcaac aacgcaaaaa
    12721 attcgggcaa gagaactctg attctttgcg agggctatat ggatgtcatc gctgtgaatc
    12781 aggcaggttt tacaaatgcc gttgcaaccc tgggaacggc tcttacaggt gaacaggctg
    12841 ttttgatgaa acggtatgcc gatgaggtga ttatctgtta cgatgccgat gaggcaggtc
    12901 aaaaggctac cgcaagggca attcctatac tcagaaattc gggacttaat gttaaggttc
    12961 ttacaattcc aagcggtaag gatcccgatg aatttatcaa atcaaagggt aatgacggtc
    13021 ctgccgcatt caaagcgttg cttgacaaat gcggtaacga tgttgaatac cgactgcaaa
    13081 agctaaaaaa tgcacacaat attcagctga cggagggcaa ggttgctttt cttgaggagg
    13141 cggcaaagct gattgcaaca atcagcagtc cgattgaacg ggatgtctat tcttcaaagg
    13201 ttgcatccga actcggagtt gacaaaaatg cattcaaaca gcaggtgtca agggtgtcac
    13261 gcagaggtga aagggctgaa gaaaagaaac aggctcgtca aattcagctt gaactcagcc
    13321 gaagaaatga caaaattaat cccgaacatt tccaaaagcc acgcagttca agtgccgaag
    13381 aagctctttt ggtttatctt ttaaacaatc ccgatgcata tgaggagatt tctgcaaggg
    13441 taaagcccga acaatttcag aatactctga tgcggaggtt ttttgaatat ttcagcgctc
    13501 gcattgagag gggagaagat cctcttacga atacggcggc agattttaca caggatgaaa
    13561 attcaaagct ttatcagctg atgtcacagg caattccggg ggcgtcaacc gctgaggcaa
    13621 tgaatgaata cataaatgtt atcaacgagg aaagctcaaa gctcaattcc ggcgatttag
    13681 ccgatgtttc aaatgaggag cttatggcat atcttgataa aataaggaaa aagaaacagt
    13741 aaacgcagga aaggaaaatg agtatggcag ctacacccga taagaaaaca gtcgtaagag
    13801 aacttatgga actcggtaag cagaaaggtc agcttacaaa ccaggacatt cttgatgcaa
    13861 tcggtgaaat tgactttgat cccgaacagc ttgaaaagct ttatgattca cttgcacagc
    13921 ttggcattga aatcgtagag gattcgggcg atattaaaat tgacgatatc gaccttggct
    13981 ttgatgaagg caaagacggc gactcacacg ctcagattgc agaaacaggc agcgtttcgg
    14041 ttgacgatcc cgtaaaggta tatcttaaag aaatcggacg ggttccgcta ctttcttccg
    14101 atgaagaaat caacctcgca atcagaatcg ctgacggtga tgttgccgca aaacagcgtc
    14161 tttccgaggc aaaccttcgt cttgttgttt caattgcaaa gcgttatctc ggcagaggca
    14221 tgcagtttct tgaccttatt caggaaggca atctcggcct tatcaaggct gtagaaaagt
    14281 ttgactatac aaagggcttt aaattctcaa catatgcaac atggtggatc agacaggcta
    14341 ttacccgtgc aattgccgat caggctcgca caattcgtat tcctgtccat atggttgaaa
    14401 caatcaacaa ggtaaaaaag gttcagagtc agctccttca ccagaacggt catgagccgt
    14461 ctgccgatga aattgccgca gaaattgata tgcctgttga taaggttcgt gaaattatgc
    14521 gtgttgctca ggagcctgtt tcacttgaaa ctcctatagg cgaggaggaa gacagccatc
    14581 tcggtgactt tatccccgat aacgatgctc ccgcacctgc cgatgccgca tcacacacaa
    14641 tgctccgtga acagctttcg gatgttcttt caaccctcac tccccgtgaa gccaaggttc
    14701 tcaaactccg tttcggtctt gaggacggca gaagcagaac ccttgaagaa gtcggcaagg
    14761 agtttaatgt aacccgtgag cgtatccgtc agattgaggc aaaggcatta aga
//
LOCUS       DAOMVE010000032        14783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_864_length_14783_cov_3.385524, whole genome shotgun sequence.
ACCESSION   DAOMVE010000032 DAOMVE010000000
VERSION     DAOMVE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 14783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14783
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_864_length_14783_cov_3.385524"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..1735)
                     /locus_tag="PLS64_03090"
     CDS             complement(362..1735)
                     /locus_tag="PLS64_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRL42850.1"
                     /translation="MAENKIPYKIYLDESEIPTKWYNVRADMKNKPAPLLNPATLKPM
                     THADLAPVFCDELIDQELDDTDAYIDIPEEIQNFYKMYRPSPLIRAYFLEKALDTPAK
                     IYYKFEGNNTSGSHKLNSAIAQAYYAKKQGLKGVTTETGAGQWGTALSMACSYFGLDC
                     KVFMVKVSYEQKPFRREVMRTYGASVTPSPSTTTEVGRKILEAHPGTTGSLGCAISEA
                     VEVATHTDGYRYVLGSVLNQVLLHQSVIGLEAKAALEKYNVKPDIIIGCAGGGSNLGG
                     LISPFMGEKLRGEKDYKFIAVEPASCPSLTRGKFAYDFCDTGMICPLAKMYTLGSGFI
                     PSANHAGGLRFHGMSSTLSQLYHDGLMEARAVEQTSVFAAAEQFARVEGILPAPESSH
                     AIRVAIDEALKCKETGEEKTILFGLTGTGYFDMVAYQKYNDGEMSDYIPTDADLQQGF
                     DGLPKVD"
     gene            2018..2935
                     /locus_tag="PLS64_03095"
     CDS             2018..2935
                     /locus_tag="PLS64_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRL42851.1"
                     /translation="MKSMTKKITATALFGVLAVSAFAGCGGAGSSSNGLSDTGSDAAK
                     FDASKTISVVTREEGSGTRDAFTELTGVLVKDGDNKTDNTTTSAVTINSTEAVITNVK
                     DNEAAIGYISLGSLNDTVKALKIGGVEATADNVKSGDYAVSRPFNIAYKGELSDVAQD
                     FVDYIMSSDGQKIVSDNGYVTVSENAAYSGKKPSGKISVAGSSSVSPVMEKLAEAYQK
                     VNTNAKVEIQTSDSSAGMQSAMGGTCDIGMASRDLKDEEKSALKVETIAKDGIAVIVN
                     NANTCDDLTLDQVKSIYTGETTVWSDIIK"
     gene            2932..3801
                     /gene="pstC"
                     /locus_tag="PLS64_03100"
     CDS             2932..3801
                     /gene="pstC"
                     /locus_tag="PLS64_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRL42852.1"
                     /translation="MRLNRFREKAMQGVFFLTAVTSIAAVVIICIFLFCNGVPAMAEI
                     GFFDFVGGQKWAPTNVPASYGIFDMIIASIYVTAGAIIIGVPIGILTAVFLARFCPAP
                     LYKVLKPATELLAGIPSIVYGFFGLVVVVPFIRDNFGGDGSSWLAASIVLGVMILPTI
                     IGVSESAIKAVPESYYEGALALGATKERSVFKTILPASKSGVLAGVVLGIGRALGETM
                     AVVLIAGNQVQIPHSVTDGLRTLTANIVLEMGYSTGLHRQALIATGVVLFVFVLLITL
                     SMQAIKSRSEKRG"
     gene            3884..4720
                     /gene="pstA"
                     /locus_tag="PLS64_03105"
     CDS             3884..4720
                     /gene="pstA"
                     /locus_tag="PLS64_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRL42853.1"
                     /translation="MKAYKRKPLSLVLLILVGLAAALSAAALIFIIAYVLVRGVPNLT
                     PELFSLEYNSDNVSCMPSIINTIVLTLVTLIISVPFGVGAAIYLAEYAKRGNKLVKVI
                     RTMAETLAGIPSIVYGLFGMLFFVYALNWGYSLLAGAFTLAIMVLPTIMRTTEEALLT
                     VPDSYREGSYGLGSGKLRTIMRIILPSAMPGILAGIILAVGRIVGETAALIYTYGSAT
                     GYAAGPLNSGRSLAVHMYVLTNEGLHTDQAWGTAVVLLVLVFAINTLSAFVAKKLGNN
                     KK"
     gene            4744..5493
                     /gene="pstB"
                     /locus_tag="PLS64_03110"
     CDS             4744..5493
                     /gene="pstB"
                     /locus_tag="PLS64_03110"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRL42854.1"
                     /translation="MDKFTIENMNLYYGKFHALKDVNIRMKKNRITAFIGPSGCGKST
                     LLKSLNRMNDLVEGCKITGDIRLDNEDIYGDMDVNILRKRVGMVFQKANPFPMSIYDN
                     IAFGPRTHGIKSKKELDRIVEESLRDAAIWDELKDRLKKSALGLSGGQQQRLCIARAL
                     AVKPEVLLMDEATSALDPISTGKIEELCLKLKNDYTIVMVTHNMQQALRISDETAFFL
                     LGEMVECNSTDAIFSNPQDKRTEEYITGRFG"
     gene            5493..6140
                     /gene="phoU"
                     /locus_tag="PLS64_03115"
     CDS             5493..6140
                     /gene="phoU"
                     /locus_tag="PLS64_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRL42855.1"
                     /translation="MREYFDKELTQLNDSLIEMGGLVEQAIKNTMKMIVDGEDLLDIA
                     REHENRINEAEKSIQSRCLHLLLHQAPVAHDLRYVSAALKMITDLERIGDQAVDIAEM
                     SVYIKDKKQVFNLTHILEMADNASKMVTEAIDAFVKSDIEVAKKVARQDDIVDELFNR
                     SKNETVELIHKNKELGSEALDIVMIAKYLERIADHAVNVAEWVAFSITGSCDLSD"
     gene            6315..6986
                     /locus_tag="PLS64_03120"
     CDS             6315..6986
                     /locus_tag="PLS64_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL42856.1"
                     /translation="MLIYIVEDDTDIRELESYAMKSSGYEVQSFGESKSFFKACTAEI
                     PQLVILDVMLPDFDGLSVLEKMRRDAVTKNVPVILVTAKGSEMDKVKGLDMGADDYIT
                     KPFSVLELISRVRAILRRCSVDDRQDVIKLGQIVFDDSKHIVTSCGEECNLTFKEYEL
                     LKYLLKNKGMALTRENIILNVWGYDFEGESRTVDMHITTLRQKLGSCGNLIKTVRNVG
                     YKAGE"
     gene            6988..8643
                     /locus_tag="PLS64_03125"
     CDS             6988..8643
                     /locus_tag="PLS64_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL42857.1"
                     /translation="MKLSKKLLKNFSVIAVVSILLTAVFSTAAFWFVFSDEQEAEVRQ
                     YTDKIISVYNSDSEIDLSKYYGIGDFRVTLIKSDGSVVSDSVDTDVNSMPNHSDRPEF
                     EQAIKDGNGSSHRMSETLNKITYYYAEKTADGSVIRVAKTIDSIYGIFLFVIPSILII
                     MIGVFVVCTILAKRSTKKIIEPIKEMADNGNGSPYDELLPLSQKIASQQRQIKRQMRR
                     LQFEKDKISTLIENMAEGFILIDVDKKVLMSNYSASKLLGADDDGVTDKTLIAFSRNE
                     VLNDCVDKALSGESKNGDTTVKGRALQIITNPVYSNGEKNGAICLIIDVSAKKKAEKM
                     RREFTANVTHELKTPLTSISGYAEIIASGLVKPDDIPNFANKIHKESGRLLSLISDIM
                     ELSQLDEKFSDEEFAPVDLAGVAAEVAEDLRSNAEKHGITITVDTKTAVINGNRNQIY
                     ELIYNLCDNAIRYNRENGSVKIITGDDNEHPFVKVADTGIGIPEKHHKRVFERFYRVD
                     KSRSKETGGTGLGLAIVKHITERHGGEISLESSEQGTTFTARF"
     gene            8893..10689
                     /locus_tag="PLS64_03130"
     CDS             8893..10689
                     /locus_tag="PLS64_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HRL42858.1"
                     /translation="MDLFMVLQLLCGLALFLFGMNSMGDGLESLSGGKLEKTLEKMTN
                     STMKGFILGAAVTAVIQSSSATTVMVVGFVNSGIMKLRQAIGIIMGANVGTTITSWIL
                     SLSGIEGDSLVMQLLKPSSFSAVFAFVGIILLMFCNSEKKKHLGTIFLGFGILMVGMD
                     QMSAAVEPLSDDPTFTGFLTLFNNPVFGILAGALLTAVIQSSSASVGILQALSKTGAI
                     SYSMAIPIVMGQNIGTCVTALISCIGAKKNAKRAAFVHLYFNIIGTLVICALFYGSNL
                     FINYGFLNDIVGPENIAVIHTAFNLLATAILLPFNKYLEKLACLTIKDKDEDSDVPFL
                     DERFLNTPSVATERAKSLAEKMARLSEGTLLGALELVDHYDEKVAETIHENEDMIDSY
                     EDVISTYLVKLSSKQLSADDSKTIQLILHTIGDFERISDHAVSIVKVAQEIHEKNISF
                     SKEAKAGLAVMVDALREIINNATVAFVDNDLALASKVEPLEQVIDRLRDKLKDAHVKR
                     LTNGTCTIELGFVFSDLITNIERVSDHCSNIAIGVIEINRNGYDAHEYLHELKNSDDI
                     QYNADYKAYKQKYTLPKEALSVREVSVGVPVN"
     gene            complement(10806..11453)
                     /locus_tag="PLS64_03135"
     CDS             complement(10806..11453)
                     /locus_tag="PLS64_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HRL42859.1"
                     /translation="MAKFKRKYTLGEEIFNSVSHGIGAGLSIAGTVVLIVTAAIHTNA
                     WGVVSSCIYGASLIILYTMSTLYHSFTNEKVKAFFRIMDHNTIFLLIAGTYTPITLYY
                     LNGVTGWILFGIVWASAIFGIVMNSINLEKARIPSIFCYIGMGWVIIFAIKPLMEHMP
                     SISGIFLIIGGVFYTVGIIFYAIKRVKYFHSIWHLFTVAGSVFHYFAILLGFFRI"
     gene            11697..12410
                     /locus_tag="PLS64_03140"
     CDS             11697..12410
                     /locus_tag="PLS64_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL42860.1"
                     /translation="MLRIEHLTKKYGDKKAVDDLSLHIQKGEIYGFIGHNGAGKTTTI
                     KSCCGILNFDEGEIYVDGVSVKEKPLECKRKIAYIPDNPDLYEFMTGIKYLNFIADIF
                     GISQSDRQARISKYAEMFEIKNALAQTIDGYSHGMKQKLAIVSALIHNPKLIIMDEPF
                     VGLDPKSSHLLKTLMREMCDNGGAIFFSTHVLEVAEKLCDKVAIIKSGKLIKSGTMDE
                     VKGDDSLEDVFLELEEKDA"
     gene            12403..14007
                     /locus_tag="PLS64_03145"
     CDS             12403..14007
                     /locus_tag="PLS64_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42861.1"
                     /translation="MLKTLIKLQFQKALARYTANSSNKKKNGKKSSFNSPAVYLALLA
                     FVGVVFAFMFYNMFSMMAPMLATSGFSWLYFLYVMGASFVISTIGCIFLSLSQLYEAK
                     DNELLLSMPIPPRSILFCRMLPLYAQNLLFCALVQVPAFAAYATNASVSVSLVVSQIV
                     ILLLVPLLSLSVSCILGWLIAFVTSRTRHKNVVTIVFSLVFFALYFYLYYQAEDLVKT
                     LAANSIGIGANIMDSAYPLYLTGLGASGDLLPLFGVAVVIIAFFAIVYAVLSHSFIKL
                     ATAKKGAKKAVYKEKTLKVSSASKALLRKERMMFTGNATYMLNSGLSALIMVGGTIYA
                     VFQLNTLKQFPSTLIEMISVYLPLVIAFVISLGPISAASISMEAKNLWLMQSLPVSPL
                     QVLTQKLKLHFIVNGVVSLVPSVVLSIMLGMKPVNIIITILIPIIFSLFSGLLGLMFD
                     LKKPKLDWNTTAEAVKQSASVMLSLLISVLAVCVPAIAYPVLLSFEIEISTEIFLACT
                     AFYFILISLPIWYWIKNKGTKVFANL"
     gene            14172..14744
                     /locus_tag="PLS64_03150"
     CDS             14172..14744
                     /locus_tag="PLS64_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42862.1"
                     /translation="MRKFRTLAVAVTLGLLLTFSGCSQASESSNTVDDIVSSVFEDET
                     TAEVTTEEPTTEEPTTEELTTEKPTEKPTEKPTQKPTKKPVKKVVEKHEDKSSQAETT
                     LEFTNVTSSVSAGSFASVSIHGAPNTTYSITVVYNSGISTASGLEDVTSDSNGNASWS
                     WKVGTRTKSGTYPITVSGGGQSESTSFSVY"
BASE COUNT         4299 a         3010 c         3204 g         4270 t
ORIGIN      
        1 ttaaatgtaa aattgaaaat tgaaaatgga aaattaatgt cgggaagaca gtttgtaatg
       61 tcaaaaaaca tattatgtcc gagcgtaaca ttgcaaccgt tcgcacaatt ctgtaggggc
      121 attcattgga cgcccgtcaa gtaacggcag aaaatttatg atgaaaataa actgtataaa
      181 tgagatacag ttaccgaaat aatacagttg aaaagaggtc agcattgctg acctcttttt
      241 tatgtaattc taaaaatgca ctcgcattct gaaaagttat gtgcgtgccg agtaatgctc
      301 gggcataata agttttagaa aaatgaaact gtcttcacga accgggtgcg agacactcgc
      361 attagtcaac ctttggcaag ccgtcaaagc cctgctggag gtcggcatct gtcggaatgt
      421 agtcgctcat ttcgccgtca ttatacttct gataagcaac catatcaaag tagcctgtac
      481 ctgtaagacc gaagagaatt gtcttttctt cgcctgtttc cttgcacttg agagcctcgt
      541 caattgcaac tctgattgcg tgactgcttt cgggagcagg aagaataccc tcaactcttg
      601 caaactgctc tgctgctgca aatacgcttg tctgctcaac tgctcttgcc tccataagac
      661 cgtcatgata cagctgagaa agtgttgaac tcataccgtg gaaacgaagt ccgcctgcgt
      721 ggttggctga cggaataaat ccgctgccga gcgtgtacat ctttgcgaga ggacaaatca
      781 tacctgtgtc gcagaagtca tatgcaaact tacctcttgt aaggcttggg catgatgccg
      841 gctcaacagc gataaacttg tagtcttttt cgccacggag cttttcaccc ataaacggag
      901 agataagacc gccgaggttt gaaccgccgc ctgcacagcc gatgataatg tcaggcttta
      961 cattgtattt ttcgagagcg gcttttgctt caagaccgat aacggactga tgaagaagta
     1021 cctgattaag cacacttccg agtacataac ggtagccgtc tgtatgagtt gcaacctcaa
     1081 cagcctcaga aatagcacag ccaaggcttc ctgttgtacc ggggtgagcc tcaagaatct
     1141 ttctgccgac ttccgttgtt gttgacggtg acggagtaac gcttgcaccg taggttctca
     1201 taacctctct tctgaaaggc ttttgctcgt atgaaacctt taccataaat accttgcagt
     1261 caaggccaaa atatgaacaa gccattgaaa gggcagtacc ccactgacct gcgcctgttt
     1321 cggttgtaac acctttaagt ccctgctttt tagcgtagta agcctgagca attgcgctgt
     1381 tgagcttgtg gcttccgctt gtgttgttgc cctcgaattt gtagtaaatc tttgcgggag
     1441 tgtcaagagc cttttcaaga aaatatgctc ttataagcgg agatggacgg tacattttat
     1501 agaagttctg aatttcttcc ggaatgtcaa tgtatgcgtc tgtatcgtca agctcctggt
     1561 caataagctc atcacagaac acgggagcaa ggtctgcgtg tgtcataggc ttgagtgttg
     1621 cagggtttaa aagcggtgca ggcttgtttt tcatatctgc acgaacattg taccattttg
     1681 tcgggatttc gctttcgtca aggtatattt tgtatgggat tttgttttca gccactaaaa
     1741 tgacctcctt ggaataatat ttctctgtaa atcttatgta agttgttttt gaaattttgc
     1801 aggaattatt ttacatcctg cacacttctg taatattgta ccacaaataa aaggctttgt
     1861 cacgcaaatt tgtaaaaatt tattttggca tacaaaacct atatttttat ttacactctt
     1921 tttacaattg acaaacagcg tattttacaa aacagtcata gactgatgtt gtacgaaaga
     1981 ggtacacaaa tgatatgaat ttaaggagaa aattcaaatg aaatctatga ctaaaaaaat
     2041 tacggcaaca gcattattcg gtgttcttgc agtttctgca tttgcaggct gcggcggtgc
     2101 aggctcttca tcaaacggtt tatccgacac aggttcggat gccgcaaagt ttgacgcatc
     2161 aaaaacaatc tctgttgtaa caagagaaga aggctcaggc acaagagacg cattcacaga
     2221 gcttacaggc gttcttgtta aggacggcga caacaagaca gacaacacaa ctacttcggc
     2281 tgtaacaatc aacagcacag aggctgttat cacaaatgtt aaggataacg aggcggcaat
     2341 cggttacatt tcactcggtt cactcaacga tacggtaaag gctcttaaaa tcggcggtgt
     2401 tgaggcaaca gccgataatg taaagtcagg cgactatgca gtaagccgtc cgttcaacat
     2461 tgcttacaag ggcgaactca gcgatgttgc tcaggatttt gttgattaca taatgagctc
     2521 agacggtcag aagattgttt cggacaacgg ttatgtaaca gtttcggaaa atgccgcata
     2581 cagcggtaag aagccgtcag gcaaaatcag cgtagcaggt tcttcttcgg tatcacctgt
     2641 aatggaaaag cttgccgagg cttatcagaa ggtaaacaca aacgctaagg ttgaaatcca
     2701 gacatccgac tcatcggcag gtatgcagtc agctatgggc ggtacatgtg atatcggtat
     2761 ggcgtcccgt gaccttaagg atgaggaaaa gagcgcactc aaggttgaaa caatcgcaaa
     2821 agacggtatc gcagtaatcg taaacaatgc aaacacatgt gatgacctca cacttgatca
     2881 ggtaaaatca atttacacag gtgaaacaac tgtttggagt gacataatta aatgagatta
     2941 aacagatttc gtgaaaaggc aatgcaggga gttttcttcc tcacagctgt aacaagtata
     3001 gcggcggttg tcataatctg tatattctta ttctgtaacg gtgttcctgc aatggcagaa
     3061 ataggcttct ttgatttcgt aggcggacag aaatgggcgc ctacgaatgt gcctgcatca
     3121 tatggaattt ttgatatgat tatcgcaagc atatatgtta ctgcgggagc aatcataatc
     3181 ggtgttccga taggaattct cacagcagta tttcttgcaa gattttgtcc tgcaccgctt
     3241 tacaaggtgc ttaaaccggc aacagagctt ttggcaggca tcccgtcaat tgtttacggc
     3301 ttcttcggac ttgtggtagt tgtaccgttc atccgtgata acttcggcgg tgacggttca
     3361 agctggcttg cggcgtcaat cgtactcgga gttatgattc ttccgacaat catcggtgtg
     3421 tcagagtcgg caatcaaggc agttcccgaa agctactacg aaggtgctct ggcacttgga
     3481 gcaacaaagg aaagaagtgt gttcaagaca attcttccgg catcaaaatc aggcgttctt
     3541 gcaggagttg ttctcggcat aggcagagcg ttgggcgaaa caatggcagt tgtacttatt
     3601 gcaggcaatc aggttcagat tccgcacagc gttacggacg gacttcgtac cctgaccgca
     3661 aacattgttc ttgaaatggg ttattcaaca ggcttgcacc gtcaggcact tattgcaacg
     3721 ggtgttgttc tgttcgtatt tgttctgctc atcacacttt caatgcaggc tataaagagt
     3781 aggagtgaaa agcgtggcta acagcgataa tataaaaaac gatgccgctg cggttgatgt
     3841 gtcacagatt aaggcaatca acaaagtatc gtttaaacag agaatgaaag cctacaaaag
     3901 aaagcccctt tcacttgtac tgctcattct tgtaggtctt gcggcggcac tttctgcggc
     3961 ggctctcata tttatcattg cctatgttct tgtgcgaggt gttccgaacc ttacccccga
     4021 gctgttctca cttgagtaca acagcgacaa tgtatcgtgt atgccgtcaa ttatcaacac
     4081 aattgttctt acgcttgtaa cccttataat ttccgtaccg ttcggtgtgg gagcggcaat
     4141 ctatcttgcc gagtatgcaa agcgtggcaa caagcttgtt aaggtaatca gaacaatggc
     4201 agaaaccctt gccggtattc cgtcaattgt ttacggtctt ttcggtatgc ttttctttgt
     4261 gtatgccctt aactggggtt attcacttct tgccggtgca tttacgcttg caattatggt
     4321 acttcccaca attatgcgta caaccgagga ggcacttctc acagttcccg attcataccg
     4381 tgagggcagt tacggactcg gttcgggcaa gctcagaaca attatgagaa ttattcttcc
     4441 gagtgcgatg cccggtattc ttgccggaat cattcttgct gtcggcagaa tagttggcga
     4501 aacagccgca cttatttata cttacggttc ggcaacaggc tatgcggcag gccctctcaa
     4561 ttcgggacgg tcacttgccg ttcatatgta tgtacttacc aacgaaggac ttcatacaga
     4621 tcaggcatgg ggtaccgcag ttgtacttct tgtacttgtt tttgcaatca atacactctc
     4681 ggcatttgtt gcaaagaaac ttggaaacaa caagaagtaa caggaaagga taattatttt
     4741 acaatggata aatttaccat agaaaacatg aatctttact acggaaaatt tcatgcgtta
     4801 aaggatgtaa atatccgtat gaagaaaaac agaatcacag ccttcatcgg tccgtcaggc
     4861 tgcggtaagt caactctttt aaagtcgctc aaccgaatga acgaccttgt tgagggttgt
     4921 aagataacag gcgacattcg ccttgacaac gaggatattt acggagatat ggatgtgaat
     4981 atcctccgca aaagggttgg tatggtgttc cagaaagcca atccgttccc gatgagcatt
     5041 tacgataaca tcgctttcgg tcctcgcaca cacggaatca aaagcaagaa agaacttgac
     5101 aggattgttg aagaatcctt gcgtgacgcc gcaatctggg atgagcttaa agacagactc
     5161 aaaaagagcg ctctcggtct ttcgggcggt cagcaacaaa gactttgtat tgcaagagca
     5221 cttgccgtaa aacccgaagt tctgcttatg gacgaggcaa cctctgcact tgatcctatt
     5281 tcaacgggta aaattgagga gctttgcctt aaactgaaaa acgattatac aatcgttatg
     5341 gtaacacata atatgcagca ggctttgcgt atttcagatg aaacagcgtt ctttttactc
     5401 ggtgaaatgg ttgaatgtaa ttcaaccgac gcaattttct caaatccgca ggataagcgt
     5461 accgaagaat acattacggg aaggtttggc taatgagaga atattttgat aaagagctta
     5521 cacagcttaa cgacagcctt attgaaatgg gcggtcttgt tgaacaggca atcaaaaata
     5581 caatgaagat gattgttgac ggcgaagatt tgcttgatat cgcaagagaa cacgaaaatc
     5641 gtattaacga ggcggaaaaa tccattcaaa gcagatgttt gcaccttctt cttcatcagg
     5701 caccggttgc acacgacctc agatatgttt ccgccgcact caaaatgatt accgaccttg
     5761 agagaatcgg cgatcaggca gttgatattg ctgaaatgtc tgtttacatc aaggacaaaa
     5821 agcaggtgtt caatcttact cacattttgg aaatggcaga caatgcctca aaaatggtaa
     5881 cggaggctat tgacgctttt gttaagagcg atattgaggt tgccaaaaag gtagcccgtc
     5941 aggatgatat cgttgacgaa ctttttaacc gttcaaagaa tgaaacggtt gaactcattc
     6001 ataagaataa ggaactcggc tcagaggctc tcgatatcgt tatgattgca aagtatcttg
     6061 aaagaattgc agaccatgcg gttaatgttg cagagtgggt tgctttttca attacaggca
     6121 gctgcgattt gtccgactga tatattttgc ttaacaaacg ggagcatagc tgtatgctcc
     6181 tgatttttgt tttattaaac tttacatttt tgcaaaaaaa gtctataatt atggcggatg
     6241 ttccgatttt aatttaagtg caggcgcccc tttgcggtgc atttaaatac acatttgttg
     6301 agggagagta ttagatgtta atttacattg ttgaagatga taccgatatc agagagcttg
     6361 aatcttatgc catgaaaagc agcggatatg aggttcagag ctttggtgaa agcaagagct
     6421 tttttaaggc ttgtacagcc gaaattcctc agcttgtaat tcttgatgtt atgttacccg
     6481 attttgacgg actttccgtg cttgaaaaaa tgcgcaggga cgctgtaacg aagaatgttc
     6541 ccgttatcct tgttactgca aagggaagtg aaatggataa ggtcaaaggt cttgacatgg
     6601 gtgccgatga ctatattaca aaaccttttt cagtccttga gcttatttca agagtccgtg
     6661 caatcctccg cagatgttct gttgatgaca gacaggatgt aataaagctc ggtcagattg
     6721 tttttgacga ttccaagcac attgtaacaa gctgcggtga ggaatgtaac ctcaccttca
     6781 aagaatacga acttttaaag tatcttctca aaaataaagg tatggcgctt acccgtgaaa
     6841 atatcattct caatgtgtgg ggatatgatt ttgagggcga gagcagaacg gttgatatgc
     6901 acattacaac cctcaggcaa aaactcggca gctgcggaaa ccttatcaaa accgtgagaa
     6961 atgtcggata taaggcgggg gaataaagtg aaactgagta aaaagctttt aaaaaacttt
     7021 tcggttatcg ctgtggtgtc cattcttctg acggctgttt tttcaacagc cgctttctgg
     7081 tttgtgttct ccgatgaaca ggaggcagag gttcggcaat ataccgataa aatcatcagc
     7141 gtttataatt ccgacagcga aatcgacctt tcaaaatatt acggaatagg cgattttcgt
     7201 gttactctca taaaaagtga cggttcggtt gtatccgaca gcgttgacac agatgttaat
     7261 tctatgccga atcacagcga ccgccccgaa tttgaacagg caatcaagga cggcaacggt
     7321 tcaagccaca gaatgtcgga aactttgaat aaaataacat actactatgc agaaaaaacc
     7381 gcagacggtt ctgtaatccg tgttgcaaag acaattgaca gcatttacgg aatttttctt
     7441 tttgttattc cgtcgattct tataattatg ataggtgtat ttgtcgtatg cacaattctg
     7501 gcaaagcgct ccacaaagaa aatcattgaa ccgattaagg aaatggcgga caacggcaac
     7561 ggctcgccgt atgatgagct tttgccgctc tcacagaaaa ttgcaagtca gcaaagacaa
     7621 atcaagcgtc agatgcgcag actgcagttt gaaaaggata aaatttcaac gcttatcgaa
     7681 aatatggcgg agggctttat tcttattgat gttgacaaaa aggtgcttat gagcaattat
     7741 tcggcgtcaa agcttctcgg tgccgatgat gacggtgtta ccgacaaaac tttgattgca
     7801 ttttcacgca acgaggttct taacgattgc gttgacaagg ctctttcggg cgagagtaaa
     7861 aacggcgaca caaccgtaaa aggaagagca ttgcagatta tcaccaatcc tgtttattct
     7921 aacggtgaaa aaaacggtgc aatctgtctt attattgatg tttcggctaa gaaaaaggct
     7981 gaaaagatgc gtcgtgaatt cacggcaaat gtaactcacg agctgaaaac tccgttgact
     8041 tcaatttcgg gttatgcaga aatcattgca agcggtcttg taaagcccga tgatatcccg
     8101 aattttgcaa ataaaattca caaggaatcg ggcagacttc tctctcttat aagcgatatc
     8161 atggagcttt cacagcttga cgaaaaattc agtgatgaag aatttgcccc tgttgacctt
     8221 gcaggggttg cagccgaggt tgccgaggat ctccgttcaa atgccgaaaa gcacggaatt
     8281 acaatcactg ttgatacaaa aactgccgtc ataaacggaa accgtaatca gatttacgaa
     8341 cttatctaca atctttgtga caatgcaatc cgttataatc gtgaaaacgg ttcggtaaaa
     8401 atcataacag gcgatgacaa tgaacatccg tttgttaaag ttgccgacac gggaatcggt
     8461 atccccgaaa agcaccacaa gcgtgtgttt gaacgctttt accgtgttga taagagccgt
     8521 tcaaaggaaa caggcggcac gggtcttgga cttgcaattg tcaagcacat taccgaaaga
     8581 cacggcggag agatttctct tgaaagcagt gaacagggaa caacatttac cgcaaggttc
     8641 tgatgtgaat atttttacaa tcttttgcag agaaaataaa ttacaagcgg tgctgtgcat
     8701 actatattgt gcagtgccgc ttttttattg ctcaaacaat acaatttcac gaatcttaca
     8761 taaaaaatgc cgtgtttcga caaatttcaa agataattac caagctttca gactttacaa
     8821 attctttaca attcattgac aatcctatga ctttagttta atattattga tgataaagga
     8881 gagtatgtat ttatggattt gtttatggtt ttacaactgc tctgcggtct ggcgctcttt
     8941 ttgttcggta tgaattcaat gggcgacggt cttgagagcc tttccggtgg taagcttgaa
     9001 aaaactttgg aaaaaatgac taacagcacc atgaaaggat tcattctcgg tgcggctgtt
     9061 actgcggtga ttcagtcctc ttcggcaaca accgttatgg ttgtcggctt tgtaaactcg
     9121 ggaattatga aactccgcca ggcaatcggt attattatgg gtgccaatgt cggcacaaca
     9181 atcacttctt ggattctcag cctttcgggt attgagggcg acagtttggt tatgcagctt
     9241 ttaaagccgt cgtctttctc tgccgtgttt gcctttgtag gaattattct tcttatgttc
     9301 tgcaattcgg aaaagaagaa gcatctcggc acaattttcc tcggattcgg tattcttatg
     9361 gttggtatgg atcagatgag tgcggctgtt gaaccgcttt cggatgatcc gacattcaca
     9421 ggcttcctca cactctttaa caaccctgta ttcggtattc ttgccggcgc attgctgacc
     9481 gcagttatac aaagctcaag cgcctctgtc ggtattttgc aggcattgtc caaaacaggc
     9541 gcaatcagct attcaatggc aatcccgatt gttatgggac agaatatagg cacttgcgta
     9601 accgcactta tttcctgtat cggtgcaaag aaaaacgcaa agcgtgccgc atttgtacat
     9661 ctttacttca atattatcgg cacactcgta atttgtgcat tgttctacgg ttctaacctc
     9721 tttatcaact acggtttcct taatgatatc gtaggtcccg agaacattgc ggttatccac
     9781 acagcattca atctgcttgc aactgcaatt cttcttccgt tcaacaagta tcttgaaaag
     9841 cttgcctgcc ttacaatcaa ggacaaggac gaggattcgg atgttccgtt ccttgatgag
     9901 cgttttctca acactccctc tgttgcaaca gagcgtgcaa agtcacttgc tgaaaaaatg
     9961 gctcgtcttt ctgagggtac gctcctcggc gcacttgaac ttgttgacca ttatgacgaa
    10021 aaggttgcgg aaacaattca tgaaaacgaa gatatgattg attcctatga ggatgttatt
    10081 tcaacatatc ttgtaaagct cagttccaaa cagctttcgg ctgacgacag taaaactatt
    10141 cagctcattc ttcacacaat cggtgatttt gagcgtatca gcgatcatgc ggtaagcatt
    10201 gtaaaggttg ctcaggaaat tcacgaaaag aacatttcgt tctcaaaaga ggcgaaagca
    10261 ggacttgccg ttatggttga cgcacttcgt gaaattatca acaacgctac tgttgctttt
    10321 gttgataatg accttgcact tgcctcaaag gttgaaccgc ttgaacaggt tatcgaccgt
    10381 ttgcgtgaca agcttaaaga cgcccatgtt aagcgtctta ctaacggtac ctgtacaatc
    10441 gaactcggtt ttgtattctc tgaccttatc acaaatattg agagagtttc cgaccattgc
    10501 tcaaacattg caatcggtgt tattgaaatc aatcgcaacg gctacgacgc acacgaatat
    10561 cttcacgaac tcaaaaattc cgatgatatt cagtacaacg cagattacaa ggcttacaag
    10621 cagaagtaca cacttccaaa ggaagctttg tctgtaagag aggtttccgt aggcgttccc
    10681 gtaaactaaa caaatctttt tataaaattt agggctacag aaaaacgaaa ggtttttctg
    10741 tagcccattt ttttgcgtgt gaaagattta cctgcggatt tttatgcggt cagctttcgc
    10801 tttcgttaaa tacgaaaaaa tccgagaaga attgcaaagt aatggaacac acttcccgca
    10861 accgtgaata agtgccatat cgagtgaaaa tattttaccc ttttaattgc gtaaaatata
    10921 atccccaccg tgtagaatac tccgcctatt atgaggaaga ttcccgaaat tgacggcata
    10981 tgttccataa gcggttttat tgcaaagatt attacccaac ccattcctat gtagcagaat
    11041 attgacggaa tccttgcttt ttcaaggttg attgagttca taactattcc gaaaattgcc
    11101 gatgcccaca caattccgaa cagaatccaa cctgtcacac cgttgaggta gtaaagagta
    11161 atcggtgtgt atgttcctgc aatcagcaga aaaattgtgt tgtgatccat tattcggaaa
    11221 aatgctttta ccttttcatt tgtgaaagaa tgatacagag ttgacattgt gtagagtatg
    11281 atgagcgatg caccgtaaat gcaagagctg acaacacccc acgcatttgt gtggattgcc
    11341 gccgtaacga taagcacaac cgttcccgca atcgacagac ctgcaccgat tccgtgtgat
    11401 acagagttga aaatttcttc cccgagtgta tattttcttt tgaactttgc caagatgaac
    11461 ctccaaaaaa taatctgtac taatattata aatcattcct gcacatattc aagatgattc
    11521 gtaatgcaaa atattccaac ggactttata tttcgtcaaa aatggtttct gttatccaaa
    11581 tttcgcagat ttattggttt gtaattcaaa cttgttgaat ttttataaca tattgacaca
    11641 agaatatttt gtgtagtata atttgcgtaa tttatttggc tataggagga gcgcttatgc
    11701 tcagaataga acatttaact aaaaaatacg gtgacaaaaa ggctgttgac gatttgtcac
    11761 ttcatattca aaaaggcgag atttacggct ttatcggaca caacggtgca ggtaagacaa
    11821 caacaatcaa atcctgctgt ggaattctca actttgacga gggcgaaatc tatgttgacg
    11881 gtgtgtcggt aaaagaaaaa cctctcgaat gtaagcgcaa aattgcttac attcccgaca
    11941 atcccgattt gtatgagttt atgacaggta ttaagtacct caattttatt gccgatattt
    12001 tcggaatcag ccagtctgac cgtcaggcaa gaatcagtaa atatgctgaa atgtttgaaa
    12061 ttaaaaatgc acttgctcag acaattgacg gctattctca cggtatgaaa caaaaacttg
    12121 caattgtttc cgcactcata cataacccca agcttatcat tatggatgaa ccgtttgtag
    12181 gtcttgatcc aaaatcatcc caccttctca aaactttgat gagagagatg tgcgataacg
    12241 gcggtgcgat tttcttctca acccatgtgc ttgaagttgc cgaaaagctt tgcgacaagg
    12301 ttgctatcat caaaagcggc aaacttatca agtcgggtac aatggatgag gtcaagggcg
    12361 atgattcgct tgaagatgta ttccttgaac tggaggagaa agatgcttaa aaccttaatt
    12421 aaactccaat ttcaaaaggc acttgcacgg tacaccgcaa attcgtcaaa caagaaaaag
    12481 aacggcaaaa aaagttcgtt taactcaccg gctgtttatc ttgctcttct cgcttttgtc
    12541 ggtgttgtat ttgcatttat gttctacaat atgttcagca tgatggcacc tatgcttgca
    12601 acatcgggat tcagttggtt gtattttctc tatgttatgg gtgcgtcgtt tgtaatcagc
    12661 acaataggct gtattttcct ttcgctctca cagctttatg aggcaaagga taatgaactt
    12721 ttgctttcaa tgcctattcc gcctcgcagt attctctttt gtagaatgtt gccactgtat
    12781 gcacaaaatc ttctcttttg tgcacttgta caggtccctg cttttgcggc ctatgcaaca
    12841 aatgcgtctg tttcggtttc gcttgtagta agtcagattg tgattctgct tttagttccg
    12901 cttttaagcc tgagcgtttc gtgcattctc ggctggctga ttgcatttgt aacatcaagg
    12961 acaagacata aaaatgttgt gacaatcgta ttttcacttg ttttctttgc actgtatttt
    13021 tacctttatt atcaggcaga ggaccttgta aaaacactcg ctgcaaattc aattggtatc
    13081 ggtgcaaata taatggacag tgcatatccg ctttatctca caggacttgg cgcatcgggc
    13141 gacttgttgc cgctttttgg tgttgctgtc gtaattattg cgttctttgc aattgtttat
    13201 gcggtgcttt ctcacagctt cataaagctt gcaacagcga aaaagggtgc gaagaaagcc
    13261 gtgtataagg agaaaaccct gaaagtaagt tctgcatcaa aggcgctttt acgcaaagaa
    13321 agaatgatgt tcacaggcaa cgcaacctat atgcttaaca gcggactcag tgcgttaatt
    13381 atggtaggcg gaacaattta tgcggtgttc cagcttaata cgctgaagca atttccaagt
    13441 acattgattg aaatgatttc agtatatctt ccacttgtga ttgcctttgt gatttcattg
    13501 ggaccgattt ctgcggcatc aatttcaatg gaagctaaaa acctttggct tatgcagtcg
    13561 cttcctgtat caccgttgca ggtgcttacg caaaagctga aacttcactt tattgtaaac
    13621 ggcgttgttt cacttgtacc ctcggttgtt ctctcaataa tgctcggaat gaaaccggtt
    13681 aatattatta tcacaattct tattccgata attttctcgt tgttctcggg acttctcgga
    13741 cttatgtttg acctgaaaaa gccaaagctt gattggaaca caaccgctga ggcagtaaaa
    13801 cagagtgcaa gcgttatgct ttcgttgttg atttcagtcc ttgccgtatg cgtacctgct
    13861 attgcgtatc cggttcttct cagctttgaa attgaaattt cgactgaaat tttcctcgcc
    13921 tgcacagcat tttattttat cctcatcagc cttccaatct ggtattggat taagaataaa
    13981 ggtacaaagg ttttcgcaaa tctttaaaat aaaaatctaa tacatttttt agaacagcct
    14041 tttggctgtt ctttttttac acagttatgt tctaaaaaat aacaattatt gattatttgc
    14101 acaaaaagct ctttacaaat aaaacaaatt gtactattat taaataaaat aattgtatga
    14161 ggtgacaata gatgagaaaa tttagaacat tagctgttgc ggtaacactg ggattgctcc
    14221 ttacattttc gggatgttcc caagcatcag agtcaagcaa tactgttgat gacattgttt
    14281 cgtctgtttt tgaagacgaa acaactgccg aagtgactac ggaagagccg acaaccgaag
    14341 aacctacaac ggaagagctg accaccgaga aaccgactga aaagcctaca gaaaaaccta
    14401 ctcagaaacc cactaaaaaa cctgtaaaaa aggttgtaga aaagcatgag gataagtcgt
    14461 cacaggctga aacaacgctc gaatttacaa atgttacatc aagcgtaagt gcaggttcgt
    14521 ttgcgagcgt tagtattcac ggcgctccaa acacaacata ttcaatcaca gttgtgtata
    14581 acagcggaat atcaacggca agcggacttg aagatgttac ttccgacagc aatggcaacg
    14641 catcgtggtc ttggaaagtc ggcacaagaa caaaatcggg aacttatccg ataaccgttt
    14701 caggcggcgg tcagagtgag agcacttcat tcagtgttta ttaaaaatcc aaattaagaa
    14761 taaacctccg aaaatttgca gat
//
LOCUS       DAOMVE010000033        13340 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1038_length_13340_cov_3.281897, whole genome shotgun sequence.
ACCESSION   DAOMVE010000033 DAOMVE010000000
VERSION     DAOMVE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13340
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_13340_cov_3.281897"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(435..1142)
                     /locus_tag="PLS64_03155"
     CDS             complement(435..1142)
                     /locus_tag="PLS64_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRL42863.1"
                     /translation="MFAALCDDDKYIIEELKKLLLTYAKENRIIIDIDEFESGEKLLN
                     SENNYDIIVLDFQLGSTDGLTVAKELRKRNVLSCIIFLTSYPHFMIDAFEVNTFRFLL
                     KPIDKSKFFKAIDDYVKIVDANYPITLIQNKELKKINSNEICFIEADGKYSNIHLNTK
                     VMHCSKTLSGVTNLLPKYCFVRTHRSFVVNLHYVKSYSSDTVYLSNGESAYLSKNYQK
                     SFRTSYMNFLKKNYVRL"
     gene            complement(1778..4108)
                     /locus_tag="PLS64_03160"
     CDS             complement(1778..4108)
                     /locus_tag="PLS64_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRL42864.1"
                     /translation="MSLKETMLSLCNGLTLVFASDEQTVNPMSLAEFFKENNVDAFNS
                     TPSRLLQYMELDEFAEAMANCKVILSGGEKYPDKLLRILREKTNATIINTYGPTEITV
                     SSNAKNLTNADEISIGRPLRNYTEYIVDSDNNLLPIGVVGELLIQGCGVALGYNKLPE
                     QTAKAFIEFNGERTYRSGDYAKWTTDGDVIILGRTDNQVKLRGLRIELGEIEKCLTAV
                     DGIKSGIALIRKVGKADAICVYYTADRQLDPDEIKSELAKSLTDYMVPSAYVQLDKMP
                     LTPNGKVNTKVLPEPKSNKSESGRLPKNELEKTFCDIFAEILELDNVFADDNFFDLGG
                     TSLTATRIVISASKKNIEVAYSDIFANPTPQTLAKFVSKDDSAEDDLENLSDYDYTNI
                     NKVLEKNNIDTFKNGELQKLGNVLLTGSAGFLGVHILYELLHKYNGKVYCMIRDKNNN
                     PAENRMNSIYYYYFEESLKERYPDRVTVISGDVTNRESFDKFIDKDINTVINCAANVK
                     HFSKGTDIEDVNLYGTLNVLDFCKKANARLVHVSTMSVGGMFVGEQGSVDKLKENQLY
                     FGQHEGSKYTLSKFLAERAILEEVSKGFNAKIMRVGTLAARNSDGEYQINFTTNTFMG
                     RLKSTLLIGKYPYEAMEMPFELSPIDFVAKAILLLAQAPKDCTVFHPFNNHTLIMGDL
                     YTEMNKIGLHSQGAEYEEYMIALDRAEQDPEKAKILSSMIAYQNMAHGQKTFTVGKSN
                     TYTMQVLYRMGFVWPVTSLDYMKRFINALRGLGFFD"
     gene            complement(4126..9105)
                     /locus_tag="PLS64_03165"
     CDS             complement(4126..9105)
                     /locus_tag="PLS64_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HRL42865.1"
                     /translation="MSNLINLLDTYKGEKRELYPLTPSQMGVYLSCMHNPKGTMYNIP
                     CTYVFDSGSLDTDRLINAVRKAVDNHPVMKIFIDNSTGSPMMKLRDSVEFDIPVVQVD
                     NLEQAGKDFVKPFDLEKDILFRFAIFECGDKSMFAMDLHHIISDGTSVSVICNDIALA
                     YDGKELEPEKFSQLDLSVFEEKLEETEEYQKSKNYYDSIFSAVESKSEITEDFAENSE
                     VEDKPNGSFELSTNGKFSVEDVRNFALANQITPYTVFLGAYEYAVAKFTNQSETTVCT
                     VTHGRFDKQLKNTVGMMVRTLPIHANIDEEDTVSDYLKKIRRNIKETVANDWFPFMKL
                     ASDYDLSADIMLAYQADIFNTFKIGGQALKLKLVPLKSAISKLNVMVFESETDFELRF
                     EYRNDLFKEETIRSFADSFLKIVEQFLKKSKLCEVELVNENQLAELDNFNKTEFPFDD
                     SKTVVDLFEEQCKKTPDKTAVVYKEKKFTYAEIDEITNRIAGYVHSKGIGKEEVVSIL
                     IPRCEYMPIASIGVLKSGAAYQPLDPSYPPERLQFMIKDANATLLIADENLLELLPDY
                     SGDILLTKDIPNLPKSDAVFAKPSPDDLFILIYTSGSTGTPKGAMLEHKNVRSFCDFH
                     IRNSDIDEHSVVSAYASYGFDACLSEMYPALIGGAELHIIEEDIRLDLVRLNRYFEDN
                     GITHAFMTTQVARQFATEIDNHSLKYLLTGGERLVPLEPPKNFELINGYGPTECTVYI
                     TTQPVDKLYHRIPVGKALDNIKLYVTDKYGRRLPTGALGELCVSGQQVSRGYLNRPEQ
                     TAKAYEKNPYCNENGYERLYHTGDIVRLTDEGKVDFIGRNDGQVKIRGFRIELSEVDK
                     IIRKYDGITNTAVIARKLDGGGQCINAYIVADRKIDIQKLNEFILEHKPPYMVPAATM
                     QIDKIPLNVNGKVDKRKLPEIKAESSKKKNSAPRELTFLEKKISAIIEKIIGHSDFDI
                     SENLINAGMTSLSVIKLAVELNKAFGFEAQVKKMMKGCSVLSIEDELQEFMFSGTANP
                     QTVKKEEKKEHKALYPLSKTQLGVYIDCMKNPYSTLYNIPSILTFSKSVDAQKLADCV
                     VKVIKAHPYIMTHLSLENDDIEQAYVDSAKPNVPVEKLTEEQLEAYKKDFVKPHNLMK
                     APLFRISVAETEKAVYLLSDFHHLIFDGASVALFFEQLKTLYEGGNIEPESYTYFDYA
                     ENEIKAESSDEFRNAEKFFDNMMKNFESASEITADLRGHAEDGALASQAVPVDMARVE
                     NFCSQHGITPAHLFLAGTFYAVSRFVNSRNVYISTISNGRSDMRLTNCFGMFVKTLAL
                     GIEIEDITSLEFVEKSKAVFTDSIENEIYPYAQLCAKYGYAPNIMYEYQLGVVDNLEI
                     DGKAVVRDYLEMNTAKFKTAIHIEDYKGKPSVVVQYNDALYSGELMRTLAKSVLCAVE
                     HIIENPNGKIRKVSLLDNAAIAQLESFKSTEIAPVKTKLLHKMFEEQVAKTPDRIALS
                     ACDGKLTYKELDRLANITANSLIEKGLEKGGKVLILLERTSKFFISLFGILKAGGAFI
                     PSCPDYPKERIDSIIEDSDADFVITEGELLGVYDRTVDVSKLLSGNNEENPNVDVQPD
                     DLAYLIYTSGSTGKPKGVMLRHIGIANYLAYSDSSMLRRLLTTVTHTAL"
     gene            complement(9124..10884)
                     /locus_tag="PLS64_03170"
     CDS             complement(9124..10884)
                     /locus_tag="PLS64_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL42866.1"
                     /translation="MSSTVLGRNAVTVNKKYTEYFLPTVLTAMATNIAMIVDSIIAGN
                     LLGQNSLAAINLMSPISQLYFSITILFGLGASSIISFAKGRRDEKQANRVFTSTFIVL
                     IALSVIFIAIQLPLADSISSLLTKDAELKSLLYQYYVPFIIGTPIYLLLMCSIHFVRA
                     DARPAFASNIVIVANAVNLGLDFLFMGAFKMGIAGSSIATVTGYAVGFVMMSTHFIMK
                     KSTLHFDFSILRNPVQFFKFLGKMVTIGLSGALGTMLITVKMLFLNTIIQSIGGSAGM
                     VSYSVCSSSQIFMSMFITGASQTMIPIIGVCLGEKDYDGVRYAFRRAARVLAVSSVVI
                     MLFICIEPEPIIKFFGITSPTDIANTVPAMRINALSFPGLSFSFLLLYYYMATQKRAI
                     STAISIINGIVILIPSALILGKFFGITGVWYSLVVAQVGTLVVVYFIDLAEKRKSGGK
                     YKNFYLLEETEDNELLALSFKGTKENAAGVSLYLSSFLSKNGIEESRANRIAVAVEEM
                     AASCAERMEGKKKFADIDIRIFADKNETIISVRDNGDEFDPLNDDKEFEMSPLTVVKA
                     ISDKVEYSRVLGFNRTIIKL"
     gene            complement(10894..11202)
                     /locus_tag="PLS64_03175"
     CDS             complement(10894..11202)
                     /locus_tag="PLS64_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRL42867.1"
                     /translation="MEINKAISNQNVIITLKGRLDTMTAPQLDDEAKSIDFDEVETVT
                     LNLKDLEYISSSGLRVILALYKNLKSKGGNLKIVNVSNTIMELFSMTGMSDYLDIEQG
                     "
     gene            complement(11370..11768)
                     /locus_tag="PLS64_03180"
     CDS             complement(11370..11768)
                     /locus_tag="PLS64_03180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL42868.1"
                     /translation="MPIVCVDAVKENLPNVIEFVTKMLGENADGKLVFELELICEEVF
                     VNICNYAYGENVGKAEIEVSKLSDCIVVKFIDSGKKFNPLEIAEPNIHAPLEERSIGG
                     LGIFMTKKLSDALSYERSNGKNIFTITKNF"
     gene            12277..13020
                     /locus_tag="PLS64_03185"
     CDS             12277..13020
                     /locus_tag="PLS64_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRL42869.1"
                     /translation="MGGKNLIKSNRKVILEGMRDGIPIGLGYLAVSFSLGIAAKTAGL
                     NAFQSFLASLLCNASAGEYSGFTVIAADSSYFVIALATLVANARYLLMSCALSQRISK
                     DTSIIHRLLLGFFVTDEFFGISISRSGKLNPFYTYGAILIGCPCWAFGSMLGTIAGNL
                     LPLRIVSALSVALFGMFLAIIVPPSRKNKTLLGIVLVSFAASFAFAYIPVINQIDEGT
                     RTIILTIVISAAAALLFPVKEEEENENVA"
     gene            13010..13315
                     /locus_tag="PLS64_03190"
     CDS             13010..13315
                     /locus_tag="PLS64_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRL42870.1"
                     /translation="MSHNPYIYILIMAAVTFAIRVLPLTLVKKQFTNTFVRSFLYYVP
                     YVTLSVMTFPAIINATHSPIAGIAAFAAGLVAAWFNAGLFKVSCVCCAVVFILEFFV"
BASE COUNT         3721 a         2694 c         2679 g         4246 t
ORIGIN      
        1 agctttgttt tgattttcag cggacttgac aggattataa acattccgaa aacaatcaaa
       61 ttagaaataa gttcaaaggc ggcagagctt tgagagttta actctatatg taaaattgaa
      121 catactaatt gatttatgga ataaataaaa aatgtcaatc ctgaaaaaaa caaggaagag
      181 aatattagca ttatctttct gttatgtccg agtgaaagag gaagaaatgt taatgcaata
      241 aaatgcaaaa ccggcagaat cacatattgt tgttcatctt ttaaaaaaca agcaaccgaa
      301 gcggttaata ttataaaaat taaagacagg gcaaaataca ttttatactt gataattgaa
      361 aatacttttg aagtaaagta aaaaactgca atcagtccca ctacaagctc cgcatagctg
      421 taaaatagca taaattataa cctcacataa ttttttttaa gaaaattcat atatgatgtc
      481 cgaaaggatt tttgataatt tttactgaga taagcagatt cgccgttact cagatacacc
      541 gtatctgatg agtatgattt tacgtagtga agatttacca caaaagaacg atgggttctt
      601 acaaaacaat actttgggag aagatttgta actcccgaaa gagtttttga acagtgcatg
      661 accttggtgt ttaaatgaat gtttgagtac tttccgtctg cctcaataaa gcagatttca
      721 tttgagttaa tttttttcaa ttccttattt tgaataagag ttatcggata atttgcatct
      781 acaattttta catagtcgtc aattgccttg aaaaattttg atttgtcaat cggtttcaaa
      841 agaaatctga atgtgtttac ttcaaacgca tcaatcataa agtggggata agatgtcaga
      901 aaaattatgc aggaaagcac attgcgtttt ctgagttcct ttgcaacagt cagtccgtcc
      961 gtactgccaa gctgaaaatc aagcacgatt atatcgtaat tattctcgga attcaaaagc
     1021 ttttcaccgc tttcaaattc atcaatatca ataattatac gattttcctt tgcatatgtc
     1081 aaaagcagct tttttagctc ttcgattatg tatttatcgt catcacacaa agccgcaaac
     1141 atattttcac cctcttactc tcaaaataga gtatcttgct ccaaactgta tttatgttgc
     1201 cttttttgtt tgctattgta atcccataca atagaataaa gggggagcaa aaagtgtttg
     1261 gacaattatt tcaatggctt ttcggttggc tctgatgcct gaaaaataaa aggcaatcgt
     1321 ttgattgctt tttatttgca aagtaatttc actcccgaaa accgcacttt tgctcctatt
     1381 cattataatt cttttacaca ataaattata atgtttctgc accttattgt caatatatgg
     1441 cattgccgtt gctgaaattt attatattat ttttttgata ttgtatgcag tgttcctttc
     1501 actcccaaaa accgctcttt tactccaagg tatatttatt ttcgactttg aatatttaaa
     1561 atgtagacag agattattat tgaaatcttg tttattaatt tgacagtaag tgcataattt
     1621 gacaagcatt taactttacc gtccttgtcc tgttttttgc agagcagggg cggtgctttt
     1681 tatgcttgcc gttattataa aatataaaaa cagcggacaa tccattgatt cggaatgtcc
     1741 gctgttttgc cttttattat gacttgatgt tgtattatta atcgaagaag ccaagacctc
     1801 ttaatgcgtt gatgaatcgt ttcatatagt caagcgaggt aacgggccac acaaatccca
     1861 ttctgtaaag aacctgcatt gtgtatgtgt tgctcttgcc gactgtaaat gtcttttgac
     1921 cgtgagccat gttctgatat gcaatcatac tcgaaagaat ctttgctttt tcgggatcct
     1981 gctcggctct gtcaagtgca atcatatatt cctcatactc tgcaccctgt gaatgaagtc
     2041 cgattttgtt catctcggtg tagaggtcgc ccataatgag agtatggttg ttgaacgggt
     2101 ggaacacggt gcaatccttc ggagcctgtg caagcagaag aattgccttt gccacaaaat
     2161 caatcggaga aagctcaaac ggcatttcca ttgcctcata tggatatttg ccgataagaa
     2221 gtgtagattt gagtctgccc ataaatgtgt tggtggtaaa attaatctga tattcaccgt
     2281 cgctgtttct tgcggcaagt gtgcctacac gcataatctt tgcgttaaag ccctttgaaa
     2341 cctcttcaag aatcgccctt tccgcaagga actttgacag ggtgtacttt gaaccctcat
     2401 gctgaccaaa gtaaagttga ttttctttga gcttgtcaac agaaccttgt tcgcccacaa
     2461 acataccgcc tacgctcatt gttgacacat gaacaagcct tgcattagcc tttttgcaga
     2521 agtcaagcac attgagagta ccgtaaaggt ttacatcttc aatgtctgta ccctttgaaa
     2581 agtgcttaac atttgcggca cagttgatga ccgtgttaat gtccttgtca ataaatttat
     2641 cgaatgattc acggtttgta acatcaccgc tgataacggt aactctgtcg ggatatcttt
     2701 ctttcagact ttcttcaaag tagtaatagt agattgagtt cattctgttt tcggcaggat
     2761 tattgttttt gtcacgaatc atgcagtaaa ccttgccgtt atacttgtga agaagttcgt
     2821 agagaatgtg tactccgagg aagccggcag agcctgtcag aagaacattg ccgagttttt
     2881 gaagctcacc gtttttgaat gtatcaatat tattcttttc aagaacttta ttgatatttg
     2941 tataatcgta gtccgaaaga ttttcaaggt cgtcttcggc tgaatcatcc ttgctcacaa
     3001 attttgcaag agtctgcgga gtaggatttg caaaaatgtc gctgtatgca acctcaatgt
     3061 ttttctttga tgcggagatt acaattcttg ttgctgtaag cgatgttccg ccgaggtcaa
     3121 agaagttatc gtctgcaaaa acattgtcaa gttcaagaat ttctgcaaag atgtcgcaga
     3181 atgttttttc aagttcattc ttcggcaacc gaccgctttc ggatttgttg cttttaggtt
     3241 cgggaagaac ctttgtgttg accttgccgt ttggtgtgag aggcattttg tcaagctgaa
     3301 catatgcgct cggaaccata taatccgtga gagattttgc aagctctgac ttgatttcat
     3361 cgggatcaag ctgtctgtcg gctgtatagt aaacgcagat tgcgtcagcc ttgccgacct
     3421 ttctgatgag agcaattccg ctcttgattc cgtcaactgc ggtcagacac ttttcaattt
     3481 caccgagttc aattcttaat cctctcagct taacctgatt gtcggttctg ccgagaataa
     3541 taacatcgcc gtctgttgtc cattttgcat agtcgcccga gcggtaggtg cgttcaccgt
     3601 taaattcgat gaatgccttt gcagtttgtt cgggaagttt gttgtagccg agtgcaacac
     3661 cgcatccctg aatgagaagc tcgcccacaa ctccgattgg aaggagattg ttgtctgagt
     3721 caacgatata ttccgtgtag ttgcgtaacg gcctgccgat tgaaatttcg tctgcattcg
     3781 taagattttt tgcgtttgaa gaaaccgtga tttcagtcgg accgtaggtg ttgattattg
     3841 ttgcgttcgt cttttcacgc agtatcctta atagcttgtc gggatatttt tcaccgcccg
     3901 agagtataac cttgcagttt gccattgctt ctgcaaattc gtcaagctcc atatattgca
     3961 aaagtcttga cggtgtagag ttgaacgcat caacattgtt ttctttaaag aattctgcaa
     4021 gtgacatcgg gtttaccgtc tgttcatcac ttgcaaaaac gagtgtaaga ccgttgcaga
     4081 gcgacagcat ggtttccttt agcgacatat caaagctgat tgtagttaca gagccgtatg
     4141 cgtgacagtt gtcaacaacc tccttaacat ggatgagtcg ctgtatgcaa ggtagtttgc
     4201 aatgccaatg tggcggagca taacgccctt tggcttgcct gttgaacccg aggtgtagat
     4261 gaggtacgca aggtcgtcag gctgtacatc aacattcgga ttttcttcat tattgcccga
     4321 aagaagtttt gaaacatcaa ccgtgcggtc atatacgcca agcagttcgc cctcggtaat
     4381 tacaaagtct gcgtcactgt cctcaataat actgtcgatt cgctccttcg gatagtcggg
     4441 acatgacgga ataaacgcac cgcctgcctt gagaataccg aaaagtgata tgaagaattt
     4501 tgaggttctt tcaagcagaa taagaacctt tccgcccttt tcaagacctt tttcaatgag
     4561 tgagtttgcc gtgatatttg caagacggtc aagctcttta taggtcagtt tgccgtcaca
     4621 agccgaaaga gcaattctgt cgggagtttt tgcaacctgt tcttcaaaca ttttgtgaag
     4681 aagttttgtc ttaacgggag caatttctgt tgacttaaag ctttcaagct gtgcaattgc
     4741 ggcgttgtca aggagtgaaa cctttctgat tttgccgttt ggattttcta taatatgttc
     4801 aacagcgcag agaacggatt ttgcaagcgt tctcataagt tcaccgctgt aaagtgcgtc
     4861 attgtactga accacaacgc tcggcttgcc cttgtaatcc tcaatgtgaa tggctgtttt
     4921 gaactttgca gtattcattt caaggtaatc acggacaacg gctttgccgt caatttcaag
     4981 gttatcaaca acaccaagct gatattcgta cataatgttc ggtgcatagc cgtattttgc
     5041 acaaagctgt gcataggggt aaatttcatt ttcaattgaa tccgtgaaaa cagccttgct
     5101 tttttcaaca aattcaagtg aggtgatgtc ctcaatttca atgccgagtg caagagtttt
     5161 tacaaacata ccaaagcagt ttgtaagccg catatcgctt ctgccgttgc tgattgtgct
     5221 gatgtaaaca tttctgctgt tcacaaatct tgaaacagcg tagaatgtgc cggcaaggaa
     5281 aaggtgggct ggagtgattc cgtgctgtga acagaagttt tcaacccttg ccatatcaac
     5341 ggggactgcc tgagatgcaa gagcaccgtc ctctgcatga cctctgaggt cagccgtgat
     5401 ttcgcttgca gattcaaaat ttttcatcat attgtcgaag aacttttcag catttctaaa
     5461 ttcatcactg ctttcagcct taatttcatt ctctgcataa tcaaagtagg tgtagctttc
     5521 aggctcaata ttacctccct catacagcgt tttaagctgt tcaaagaaga gtgcaacgga
     5581 cgcaccgtca aagataaggt ggtggaaatc cgaaagcaga tataccgcct tttcggtttc
     5641 tgcaactgag attctgaaca gcggtgcttt cataagattg tgcggtttta caaaatcctt
     5701 tttgtaggct tcaagctgtt cttccgtaag cttttcaacg ggaacattcg gttttgcaga
     5761 gtcaacatat gcctgctcaa tatcatcgtt ttcaagtgaa aggtgagtca taatatatgg
     5821 gtgagccttg attaccttta caacgcagtc ggcaagcttt tgtgcatcga ctgattttga
     5881 aaatgtgaga attgacggaa tattgtaaag tgtgctgtac ggatttttca tacagtcaat
     5941 atacacgccg agctgagttt ttgaaagggg atagagagcc ttgtgttctt tcttttcttc
     6001 ttttttgaca gtttgcggat ttgccgtacc gctgaacata aactcctgaa gttcgtcctc
     6061 aatcgaaaga accgaacagc ccttcatcat ctttttaacc tgtgcctcaa agccgaatgc
     6121 cttgttgagc tcaactgcaa gcttgataac cgaaagcgag gtcatacctg cgttgatgag
     6181 attttcggaa atatcaaaat cgctgtgtcc gataatcttt tcgataattg cggaaatttt
     6241 cttttcaagg aatgttagtt ccctcggagc ggaatttttc ttttttgaag attctgcctt
     6301 gatttcggga agcttgcgct tgtcaacctt gccgtttaca ttgagcggaa ttttgtcaat
     6361 ctgcatcgta gcggcgggta ccatatacgg cggcttgtgt tcaagaataa attcgttgag
     6421 cttttgaatg tcaatttttc tgtcggcaac aatgtatgcg ttaatgcatt gtccgccgcc
     6481 atcaagcttt cttgcgatta cagctgtgtt tgtaatgccg tcatatttgc ggataatctt
     6541 gtcaacctcg gaaagctcaa tgcggaatcc tctgatttta acctgaccgt catttcgtcc
     6601 gataaaatca accttgccct catctgtaag gcgaacaatg tcgcctgtgt gataaagcct
     6661 ttcgtaaccg ttttcattgc aatacgggtt cttttcgtat gcttttgcag tctgttcggg
     6721 acggttaaga taacctcttg aaacctgctg acctgataca cagagctcgc ccaaagcccc
     6781 tgtcggaagc cgtctgccgt atttgtctgt tacataaagc ttgatattgt caagcgcctt
     6841 gccgacagga attctgtggt agagcttgtc aacgggctgt gttgtgatgt aaaccgtaca
     6901 ttctgtcgga ccgtaaccgt ttatgagttc aaaattcttt ggcggttcaa ggggaacaag
     6961 ccgttcaccg cccgtaagaa gatatttgag cgagtggttg tcaatttcgg ttgcaaactg
     7021 tcttgcaacc tgtgttgtca taaatgcgtg ggtgattccg ttatcttcaa aatatctgtt
     7081 cagccttaca aggtcaagtc tgatgtcctc ctcaataatg tgaagctcgg caccgccgat
     7141 aagcgcagga tacatttcac tgaggcaggc gtcaaaaccg tagcttgcat atgccgaaac
     7201 aacgctgtgt tcgtcaatgt cagagtttct gatatgaaaa tcgcagaatg aacgaacatt
     7261 tttgtgttca agcatagcac cctttggagt gcctgttgaa cccgatgtat agataagtat
     7321 aaacaaatca tcgggtgagg gttttgcaaa aactgcgtct gatttcggaa gattcggaat
     7381 atcctttgta agaagaatat caccgctgta atcgggcaaa agctcaagca gattttcgtc
     7441 cgcaataaga agcgttgcgt ttgcgtcctt aatcataaac tgaagtcttt cgggcggata
     7501 tgacggatca agcggttgat atgccgcacc cgatttcagc acaccgattg acgcaatcgg
     7561 catatattcg catctcggaa tcagaattga aacaacctct tctttaccga taccctttga
     7621 gtgaacataa cctgcaattc ggtttgtaat ctcatcaatt tcagcgtagg tgaacttctt
     7681 ttctttatat acaaccgctg ttttgtcggg agtttttttg cattgctcct caaacaaatc
     7741 aacaacggtt ttgctgtcgt caaacggaaa ttccgttttg ttgaagttat caagctctgc
     7801 aagctgattt tcgtttacaa gctcaacttc gcagagcttg gattttttaa gaaactgctc
     7861 aacaatcttt aaaaagctgt cggcaaacga acgaatagtt tcctctttga ataaatcgtt
     7921 tctgtattca aaacgcaatt caaaatctgt ttctgattca aataccataa cattcaattt
     7981 tgaaatggca ctttttaacg gaacaagttt taattttaga gcctgaccgc ctattttaaa
     8041 ggtgttgaat atgtcagcct gataggcaag cataatgtcg gcagagaggt catagtcaga
     8101 cgcaagtttc ataaacggaa accaatcgtt tgcaactgtt tcctttatgt ttctgcgtat
     8161 ttttttgagg tagtccgaaa ctgtatcttc ttcgtcaata tttgcgtgga taggaagtgt
     8221 gcgaaccatc atgccgaccg tatttttaag ctgtttgtca aatcttccgt gggtaactgt
     8281 gcaaaccgtt gtttcactct gattcgtaaa ctttgcaact gcgtattcat atgcaccgag
     8341 gaaaacagtg tacggtgtaa tttggtttgc taaggcaaaa ttgcgaacat cctcaacgga
     8401 gaatttgccg ttcgtggata attcaaaaga accgttcggt ttatcctcaa cctctgaatt
     8461 ctctgcaaaa tcttccgtta tttccgactt tgactcaaca gccgaaaaaa tgctgtcata
     8521 gtagttcttt gatttttgat attcttcggt ttcttcaagc ttttcctcaa aaacagaaag
     8581 atcaagctga gaaaactttt cgggttcaag ctcttttccg tcatatgcaa gcgcaatgtc
     8641 attgcagata accgaaacag atgtgccgtc agatattatg tggtgaaggt ccattgcaaa
     8701 catagatttg tcaccgcatt caaaaattgc aaatctgaac agaatgtctt tttcaaggtc
     8761 aaacggctta acaaaatctt tgcctgcctg ttcaagattg tcaacctgta cgacaggaat
     8821 gtcaaattca acgctgtcac gaagtttcat catcggactt cctgttgaat tgtcaatgaa
     8881 aattttcata acagggtgat tgtcaacagc ttttcttacg gcatttatca gcctgtcggt
     8941 atcaagactt ccactgtcaa aaacataggt gcagggaatg ttgtacattg tgccttttgg
     9001 gttgtgcata cacgaaagat aaacacccat ctggctcggt gtaagcggat aaagctctcg
     9061 cttttcacct ttgtatgtat caagcaagtt gattaaatta ctcataatat tcaactccgt
     9121 ttgttaaagt ttaattattg tgcggttgaa accaagcacc cttgaatatt caaccttatc
     9181 cgaaattgcc tttacaaccg tgagaggact catctcaaat tccttatcgt cattgagcgg
     9241 atcaaattca tcaccgttgt cacgaacgga aattattgtt tcgtttttat cggcaaaaat
     9301 tctgatgtcg atgtctgcaa atttcttttt gccttccatt ctttcggcac agcttgcagc
     9361 catttcttca acggcaactg caatcctgtt tgcacggctt tcttcaattc cgttttttga
     9421 aagaaaagag gagaggtaga gcgaaacgcc tgccgcattt tcttttgttc ctttgaacga
     9481 aagggcaaga agctcgttgt cctcggtttc ttcaagaagg taaaagtttt tatactttcc
     9541 gcccgatttt ctcttttcgg caaggtcaat aaaatacaca actacaagtg ttcccacctg
     9601 tgcaaccacg agtgaatacc atacgcccgt gatgccgaaa aatttgccga gaattaaagc
     9661 tgacggaatg agtatgacga ttccgttgat gattgaaatt gcagtagaaa tagctctttt
     9721 ctgcgttgcc atatagtagt aaagcaacaa aaacgagaat gaaagtccgg ggaatgacaa
     9781 tgcgttgatt ctcattgccg gaactgtgtt tgcaatgtct gtgggtgaag taattccgaa
     9841 aaatttaatt atcggttcag gctcgataca gatgaaaagc ataattacaa cgcttgaaac
     9901 agcaagcacc cttgcggctc tgcggaatgc atagcgcaca ccgtcatagt ccttttcacc
     9961 aagacaaacc ccgataatcg gaatcattgt ctgacttgca cccgtgatga acattgacat
    10021 aaaaatttgg cttgacgagc ataccgaata tgaaaccata cccgcacttc cgcctatgct
    10081 ctgaataatt gtgtttaaaa acagcatttt aactgttatc agcattgtac ctaatgctcc
    10141 cgaaagtccg attgtaacca tttttccgag aaacttaaag aattgaacgg gatttctgag
    10201 aattgaaaag tcaaagtgca atgtgctttt cttcatgatg aagtgtgttg acatcatcac
    10261 aaaccctacc gcatagcctg ttacggtcgc aattgacgag cctgcaatgc ccattttgaa
    10321 agcacccata aacaggaagt caagtccgag atttacggca tttgccacaa ttacaatgtt
    10381 tgatgcaaac gcaggccttg cgtctgctct tacaaagtga attgaacaca tgagcaaaag
    10441 gtaaatcggt gtgccgatta tgaacggaac atagtattga tacaacagcg atttaagctc
    10501 tgcgtccttt gtaagcaacg agcttatcga gtcggcaagc ggcagctgaa ttgcaataaa
    10561 aattacgctg agtgcgataa gtacaataaa tgtagaagta aaaacacggt tagcctgctt
    10621 ttcgtcacgc ctgccctttg caaatgaaat tatcgaggac gcaccgagtc cgaaaagaat
    10681 agtaatcgaa aaatataact gcgaaatagg ggacataagg ttgattgccg caagtgagtt
    10741 ttgccccaga agattgcctg caataattga gtccacaatc atcgcaatgt ttgttgccat
    10801 agccgtcaga actgtaggaa gaaaatattc cgtgtatttt ttgttgacgg taacggcatt
    10861 tctgccaagt acggtactgc tcattttaac tccttatccc tgctcaatgt caaggtagtc
    10921 agacatcccc gtcattgaga acagctccat aattgtgttg cttacattga caattttaag
    10981 atttccgccc ttgcttttta ggtttttata aagtgcaaga ataaccctca gtcccgagct
    11041 tgaaatgtac tcaaggtctt taaggttgag agttacggtt tcaacctcgt caaaatcaat
    11101 gctctttgcc tcgtcatcaa gctgcggtgc tgtcattgtg tcaagacgac ccttgagtgt
    11161 gataataaca ttctgattgc ttatagcctt attgatttcc atagttagtt cctccaataa
    11221 attttattct taatttcaat cgttcaacat ttccattata gtgtcaatac cgtgacaaaa
    11281 tactgacaat ctgattatta taacctgatt ttggaaacat tgtattattc ggttttgaat
    11341 tttgagtgat gaataattga ttctttaaat tagaaattct ttgtaattgt aaaaatgttt
    11401 ttgccgttag atctttcata ggaaagagcg tcggaaagtt tctttgtcat aaaaattccc
    11461 agaccgccga tgcttcgttc ctcaagcgga gcgtgaatgt tcggctctgc aatttcaagc
    11521 ggattgaatt tttttccgct gtcaatgaat tttacaacaa tgcagtctga aagtttacta
    11581 acttcaattt cagccttgcc gacattttca ccgtaagcat agttgcagat gtttacaaac
    11641 acttcctcgc aaatcagttc aagctcaaaa acaagcttac cgtctgcatt ttcaccaagc
    11701 atttttgtca caaattctat aacattcggc agattttcct taacggcatc aacgcaaacg
    11761 attggcataa ttctcactca ctttcaaaac acctgtttca acataatgta cagttttata
    11821 caggtactta atcacaaaaa ttatacaaca ttttgaagaa gaaatctata gaatttgaaa
    11881 aaatattgct aaattgtgaa aatattttaa ttaattgcgt ttacctcgca ttcttttgaa
    11941 aaaacaaaac cgtgcaaaac aaaaatcagt tttgcacggt tttgtggttt ttatttactt
    12001 tttccaattc ccctattaat ttcgtcaagt gataatccat attgtaaaac ctgaaaaagc
    12061 caaaatcaat aatagcctaa aattagtttc taaatttggc attactcaca aagaaaagag
    12121 gtaagttttg ttgcacttta cctttttgaa aagcggttgt aaatttgtgt aatatattat
    12181 ttacttttgc cgagttaaat attataataa agggtatgct tgcaaaatat atctgcaata
    12241 ttttgtaaaa aatgaattta aagtatggag ttaaaaatgg gtgggaaaaa tttaataaaa
    12301 tctaaccgaa aggttattct tgagggaatg cgtgacggta ttccgattgg actcggctac
    12361 cttgccgttt cgttttcact cggcattgcc gcaaaaactg cgggacttaa tgcgtttcaa
    12421 agctttcttg caagcctttt gtgcaacgca tcggcaggtg aatattcggg ctttaccgta
    12481 attgcggctg attcgtcata ttttgtaatt gcgcttgcaa cccttgttgc caacgcaaga
    12541 tatcttctta tgagctgtgc gttaagtcag cgtatttcaa aggacacctc gattatccac
    12601 aggctgttgc tcggattttt tgttacagac gaatttttcg gaatttcaat ttcacggtcg
    12661 ggcaagctga atcctttcta tacctacggt gcaattctca taggttgccc gtgttgggca
    12721 ttcggcagta tgctcggcac aattgcaggc aatctgttgc cgcttcgtat tgtcagcgca
    12781 ctaagcgttg cacttttcgg aatgttcctt gcaatcatcg ttccgccgtc aagaaagaac
    12841 aaaacacttc tcggaattgt acttgtcagc tttgcggcaa gttttgcatt tgcatatatt
    12901 cccgtcatca atcagattga cgagggtaca cgcacaatta ttcttacaat cgtaatttcg
    12961 gcagcggcgg cattgctgtt ccctgtcaaa gaagaggagg agaacgaaaa tgtcgcataa
    13021 tccctatata tacatactca ttatggcggc tgtaacattc gcaatccgtg ttcttccgct
    13081 tacacttgtt aaaaagcagt ttacaaacac atttgtgcgt tcgtttctct actatgtgcc
    13141 gtatgtaacg ctttcggtaa tgacatttcc ggcaataatc aacgcaactc acagtccgat
    13201 tgcaggcata gcagcctttg cggcaggact tgtagcggca tggttcaacg caggtctttt
    13261 caaggtttca tgcgtatgct gtgcggttgt gttcattctt gaattttttg tgtgacattt
    13321 ttgacgcata gcctgttgtt
//
LOCUS       DAOMVE010000034        13174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1067_length_13174_cov_2.804101, whole genome shotgun sequence.
ACCESSION   DAOMVE010000034 DAOMVE010000000
VERSION     DAOMVE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1067_length_13174_cov_2.804101"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..995
                     /locus_tag="PLS64_03195"
     CDS             126..995
                     /locus_tag="PLS64_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRL42871.1"
                     /translation="MHPFINFIFFALTIGFTMFIMNPVCLAVSSLCALLTALYLNGKK
                     AVRLSLVFLLPMILLIVLVNPVFNHEGMTILTYFPWDNPLTLESIVYGIASAFLLSST
                     VLWFSSFNAVITSDKVVFLFGRIMPSLSLVISMALRFIPRFSAQMKLVRNAQHTIGRD
                     INEGTLFQRIRNAVKILSIMITWSLENAIETADSMKSRGHGLKGRTSYSLYKFDKRDA
                     FMLCTILFTGLVLMILLFTNAIKFRYYPSIKGDLFTVQSIIFYIFYTFLMLIPLSVNI
                     GEGIKWKHMQSKI"
     gene            968..>2656
                     /locus_tag="PLS64_03200"
     CDS             968..>2656
                     /locus_tag="PLS64_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL42872.1"
                     /translation="METYAIKNLSFRYPETGIDVLKDITFSVNEGEFITVCGPSGCGK
                     STLLRHLKPDLSPHGVLSGEIFFEEKPISDLSHREQSSKIGFVMQSPENQIVTDKVWH
                     ELAFGLESLGYDNNTIRKRVAETASFFGIQNYFEKSVLELSGGQKQILNLASIMAMQP
                     SVLILDEPTSQLDPIAASEFLHCVSRINHELGTTVIITEHRLDEVLPLSDRVLVIENN
                     GISAFDSPQKVGKILKEKDSKTFLSMPAPMQIWQAVTENDNTDCPVTVPSGKKWLSEY
                     AQNHKMYELTPENIQKHSDKEAVSLNDIWFRYEKSGTDVLKGLSLKIYQGEFAAILGG
                     NGMGKSTALSIICSLNKPYRGKVEISPLNKNSFDTLVAVLPQNPQTLFLKKTVLEDLY
                     EVFDGRKISKEEKERRVTAAVKLCRLESLCNRHPYDLSGGEQQRAALAKILLIRPQIL
                     LLDEPTKGLDAEFKIEFAQIIYDLNKAGITVLMVSHDVEFCAVYPSRCLMFFNGEVVS
                     EGTPRNFFSSNSFYSTSASRMSKGIIDNAVSSNDVIYACTGKNRDIQINRNTPDI"
     assembly_gap    2657..2666
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2667..3593
                     /locus_tag="PLS64_03205"
     CDS             <2667..3593
                     /locus_tag="PLS64_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRL42873.1"
                     /translation="KAENKKLSVFKKIFGFFGAVLFILGLIVNLEYIPNFSAKTLPTW
                     FNWGIIGVSVALLMIAFGTKSKRPIDLPRKSSKLSKRTVSMAIMVLLAIPVTIFVGMT
                     YLQDQKYLFISLLVMIECMIPFFLVFEGRHPKARELVIISVLCAIAVAGRLAFTMLPQ
                     FKPVVAIVIISGVAFGGESGFLVGAVSMLVSNLMFGQGPWTPWQMFAAGIIGFIGGIL
                     FKKGLLGRTRTSLCIYGFIATMVIYGGLMNLYSALTSHSAFNLNMLITFYVQGFPMDI
                     VHAVSTVIFLFFGAEPMLEKLDRIKVKYGLIE"
     gene            3789..5198
                     /locus_tag="PLS64_03210"
     CDS             3789..5198
                     /locus_tag="PLS64_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRL42874.1"
                     /translation="MGGFMGVAAKQDCVFDLYFGIDYHSHLGTRRGGMVVYDKEKGFD
                     RSIHNIENAPFRTKFDKDMQSMKGKFGIGCISDYEPQPLIVRSHHGTYAITTVSKINN
                     TDELVEEIFEKGGSHFLEMSGGEINATELIATLINQKENLVDGISYALEKVDGSVSLL
                     LMNKNGIYCARDKYGRTPVVIGKKEDAFCVAFECFSYKNLGYNDYKELGPGEITVITD
                     QNCITLKKPGDKMKICTFLWVYYGYPASSYEGTSVEQMRYNCGAKMAERDSVKPDIVA
                     GVPDSGIAHAVGYANASGIPFSRPFIKYTPTWPRSFMPTIQSQRNLIAKMKLLAVEDL
                     IRGKSLLLIDDSIVRGTQLRETTEYLFESGAKEVHIRPACPPLVYGCKYLNFSRSSSE
                     MDLITRRVIERLENGNVTDEILQEYTNPDSEKYEQMVDEICKELKFTSLRFNRLDDML
                     DSCGIDKCKLCTYCWDGKE"
     gene            complement(5268..6140)
                     /locus_tag="PLS64_03215"
     CDS             complement(5268..6140)
                     /locus_tag="PLS64_03215"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HRL42875.1"
                     /translation="MEKPGRNDPCWCGSGKKYKKCHIDFDEKIEEFEGAGHIVPSHEI
                     LKNAEQIEKIKESAKINIAVLDYVAEHIKAGISTAEIDKWVYDITTKMGGVPAPLNFE
                     GFPKSVCTSINNEVCHGIPSEDVIIKDGDIINVDVSTNLNGYFSDSSRMFCIGNVSEE
                     NRKLVEETKNAVYEGLKQVKPWGFLGDMGQAVNDYVKSKGYSVVREVGGHGIGLEFHE
                     DPWVSYISKKGTEMLMVPGMIFTIEPMVNAGKPDIFVDEDNGWTIYTEDNSMSAQWEI
                     QVLVTEDGYEIIAY"
     gene            6332..7042
                     /locus_tag="PLS64_03220"
     CDS             6332..7042
                     /locus_tag="PLS64_03220"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRL42876.1"
                     /translation="MNKKIPTFYALCFILFAIFCIIMVSVFFRLNPTKSVMTENKTEP
                     IIVIDAGHGGEDGGAVSESGVLEKDINLSIANNSADLLKIFGYKVVQTRTDDVSLDNG
                     EDSVHSRKVADLKKRLEIFNSDKNNIVISIHQNKFSQSKYYGTQIFYSPNNSQSQNLA
                     ECIRNSVKKCLQPDNERQCKKADGSIYLLKKAENPSVIVECGFISNPDELAKLQSDEY
                     QKEMATAIITGFLDYTHQ"
     gene            7061..8428
                     /gene="radA"
                     /locus_tag="PLS64_03225"
     CDS             7061..8428
                     /gene="radA"
                     /locus_tag="PLS64_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRL42877.1"
                     /translation="MASKIKSVYICSECGYESPKWYGKCPSCGEWNTMNEEIKDTSKQ
                     SATAKTRSFATYSKPYAISDISTEDEHRYDTGCGELNRVLGGGIVKGSLILLGGDPGI
                     GKSTVLMQICQYLGDTLKILYISGEESKRQLKLRAIRLGVTSPNLYVMTETDVEVICE
                     QIKNLKPDLVMIDSIQTMNLSELSSSPGSITQVRESANMLMRTAKGLDIPMFIVGHVN
                     KEGSIAGPKVLEHIVDTVLHFEGDKQMSCRILRAVKNRYGSTNEIGVFEMTDKGLNEV
                     ANPSVMLLSGRPKNVSGTCVTCTIEGSRPILAETQGLATQSGYGNARRMVTGYDYNRL
                     SMVLAVLEKRAGYYFSNCDTYINIVGGLRVDEPAIDLSIAMALISSLKDTPIDENAVV
                     FGEIGLAGEIRAVSNAQMRINEAVRLGFTKIILPYHNLKGVSCDDAELIGVKNIRQAF
                     EAVGQ"
     gene            8425..9120
                     /locus_tag="PLS64_03230"
     CDS             8425..9120
                     /locus_tag="PLS64_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42878.1"
                     /translation="MSSFVISNKYPCFADELSKMGHNVIFSDTVKAFPQPEQAHADMQ
                     ILTINNTVFVLQECEKLKTLPYKENLIICKSKAGKKYPENILLNFLFFNNKLYGKVSA
                     IDPTLYKYCVKNDIEIVNINQGYARCSTLMLNNRTAVTADISIKNALEKDGAEVLLIS
                     SGDIKLEGYDYGFIGGASGKISDNTVVFFGNAEMHPCYSSIRELCLKNKIEIKILCKN
                     MPLTDVGGIVKIL"
     gene            complement(9117..10100)
                     /locus_tag="PLS64_03235"
     CDS             complement(9117..10100)
                     /locus_tag="PLS64_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRL42879.1"
                     /translation="MAVDFRDEAPAILKDYLIYKQTIKNMSKKTVDEYFIDLRTFFRF
                     LKVMRGLVPDGTEFDEIKIDDVDLDLIKTVNLELAYDYMNFLYRDRNNKSASRARKCS
                     SLKGFFKYITNNKHLLDTNPVEQLESPKNKKALPKYLTLEQSIELLNAVDGNNKQRDY
                     CILTLFLNCGLRVSEMAGLNYNDIHSDGTMRVVGKGNKERIVYLNSACIEAYNEYMKV
                     RPIDEVKDKKALFISRNHRRMSVKTIQAMVYKYLEKIGLDSQGYSCHKLRHTAATLMY
                     QQGGVDVRVLKEVLGHENLGTTEIYTHLSSEQMKNAADSNPLAKIKPKKKV"
     gene            complement(10399..11730)
                     /gene="gdhA"
                     /locus_tag="PLS64_03240"
     CDS             complement(10399..11730)
                     /gene="gdhA"
                     /locus_tag="PLS64_03240"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRL42880.1"
                     /translation="MSYVSEQLEKLKAKNANEPEFIQAATEVLTSLEPVFEQNPKYEE
                     NGILERITEPERVIMFRVPWVDDNGKVQVNRGFRVQFNSAIGPYKGGLRLHPSVNLGV
                     IKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDREVMAFCQSFMTELCKHIGADTD
                     VPAGDIGTGAREIGYMFGQYKRIRNVYEGVLTGKGLNWGGSLARTEATGYGLLYLTEA
                     MLKDNGKDIKGATVCVSGAGNVAIYATEKATELGAKVVTMADSTGWIYDAEGIDLDAI
                     KEIKEVRRQRLTEYKNYRPNSEYHEGKFDWSVKCDVALPCATQNELNEEDAKRLIANG
                     CYAVAEGANMPTTLEATKLLQKSGILFAPGKAANAGGVATSALEMSQNSQRLSWTFEE
                     VDAKLKNIMVNIYKNISAAAKKYGHEGDYVVGANIAGFEKVADSMIQQGVC"
     gene            12143..12352
                     /locus_tag="PLS64_03245"
     CDS             12143..12352
                     /locus_tag="PLS64_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004844861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL42881.1"
                     /translation="MDYCKRLKELRVKNGYTQGQIAFMLHTRQEQYSKYENGKRELPI
                     RHLITLCCLYKTSSDYILGIEKFVK"
     gene            complement(12416..13060)
                     /locus_tag="PLS64_03250"
     CDS             complement(12416..13060)
                     /locus_tag="PLS64_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRL42882.1"
                     /translation="MSEAFRNCIFLALSGGFQDAYTYFTRDGVFANAQTGNVVLMSTN
                     FMTGKWGEGLKYLFPILAFALGVLVADLIQKKFKYFKKIHWRQGILLFEIIILFAVGF
                     MPKSMDTVAAVIVSFSCAMQVQTFRKVRGYSYASTMCIGNLRSGTAALSGFIHERKPE
                     QLRQAMYYFGVIFFFAVGAGLGGNLSLSFGTHIIWVSCGLLLVSFLMMFKEKIQ"
BASE COUNT         4210 a         2617 c         2443 g         3894 t
ORIGIN      
        1 ggctgttcac gatatgtgct gaaaaacggc gacgatgtgg tctggtgcta cacttgtaac
       61 ttaggctatg atgtcggcgg aggatacgca acaggagaat aatgatgaag gatacttttt
      121 caaatatgca cccctttatc aattttatat tttttgcact tacaataggg ttcacgatgt
      181 ttatcatgaa ccctgtttgt cttgctgtaa gttctttatg tgcgctttta actgctttat
      241 accttaacgg caaaaaagct gtgagattgt cacttgtttt tcttttacct atgatactct
      301 tgatagttct cgtaaatcct gtttttaatc atgaaggaat gactatactc acatattttc
      361 cgtgggataa cccactaacc ctcgaatcaa tcgtttacgg tattgcaagt gcatttttgc
      421 tttcgtcaac agttttatgg ttttcttcat tcaatgcagt aatcacttcc gacaaggtag
      481 ttttcctgtt tggcagaata atgccgtcat tgagtctggt gatttcaatg gcattgcgtt
      541 ttattccaag gttttcggca caaatgaagc ttgtgcgtaa tgcacaacac acaataggca
      601 gagatataaa cgagggaaca ttgtttcaaa gaataagaaa cgcagtaaaa atcctttcga
      661 ttatgataac atggtcgctt gaaaacgcaa ttgaaacagc cgattcaatg aaaagcagag
      721 gtcacggtct taaaggcaga acatcctatt cactttataa atttgacaaa agagacgcat
      781 ttatgctttg cacaatcctt tttaccggat tggtacttat gattttgctt tttacaaacg
      841 caataaaatt cagatactat ccgtcaatca agggcgattt gtttacggtg caaagtatta
      901 ttttttatat attctacaca tttttaatgt tgattccgct atcggtaaat ataggagagg
      961 ggataaaatg gaaacatatg caatcaaaaa tctgagtttt cgataccccg aaaccggtat
     1021 tgatgtactt aaagatatta ctttcagcgt aaatgagggc gagtttatca cagtctgcgg
     1081 tccgtcaggc tgcggaaaaa gcacacttct cagacatctg aaacccgatc tttcaccgca
     1141 cggtgttctt tcgggagaaa tattctttga agaaaaacct atatcagacc tttctcacag
     1201 ggagcaaagc agtaaaatcg gctttgttat gcaatcacct gaaaatcaga ttgttaccga
     1261 taaggtatgg cacgaacttg ctttcggact tgaaagtctt ggttatgaca acaatacaat
     1321 ccgaaaaaga gttgccgaaa cagcttcatt tttcggaatc caaaattatt ttgaaaaaag
     1381 tgttctcgaa ctttcgggcg gacaaaagca aattttaaac cttgcttcaa ttatggcaat
     1441 gcaaccgtcc gttctcattt tggatgaacc gacaagccaa ctcgatccga ttgcggcaag
     1501 cgaatttttg cactgcgttt cacgcataaa tcacgaactg gggacaacgg ttataataac
     1561 ggaacacaga cttgatgagg tgcttccgct ttcagacagg gtgcttgtca tcgaaaacaa
     1621 cggaatttca gcttttgact ctcctcaaaa ggtcgggaaa attcttaaag aaaaagacag
     1681 taaaacattt ctttctatgc ctgctccgat gcagatatgg caggctgtta cagaaaatga
     1741 caataccgac tgtcccgtaa ctgttccaag cggcaaaaag tggctttcgg aatatgccca
     1801 aaatcataaa atgtatgagc tcacgcccga aaacattcag aaacattctg ataaagaagc
     1861 agtaagtctt aatgatattt ggttcaggta cgaaaagtcg ggtacagatg tactaaaagg
     1921 tctttcacta aaaatatatc agggggaatt tgcggctata ctcggcggta acggaatggg
     1981 aaaatcaacc gctttgtcaa ttatctgttc acttaacaag ccatacagag gcaaagttga
     2041 aatctctccg cttaacaaaa attcttttga cacgctcgta gcagtattac cccaaaatcc
     2101 gcagacactg tttttgaaaa aaactgtact tgaagattta tacgaagtat ttgacggcag
     2161 aaaaatcagc aaggaggaaa aagaaagacg ggttaccgca gcggtaaagc tgtgcagact
     2221 tgaaagtctc tgcaatcgcc atccttatga tttatccggc ggtgaacagc agagagcggc
     2281 tcttgcaaaa attcttctca taagacctca aattcttttg cttgatgaac ctacaaaggg
     2341 acttgatgcc gaattcaaga ttgagtttgc tcagattata tacgacctca acaaagccgg
     2401 cataaccgtt ctcatggtaa gtcacgatgt agagttctgt gctgtatatc caagccgctg
     2461 tctgatgttt tttaacggtg aagttgtgtc ggaaggtaca ccgagaaact tcttttcgtc
     2521 aaacagcttt tattccacct ctgcaagccg aatgtcaaaa ggcataatag ataacgcagt
     2581 atcatcaaat gatgttatat atgcctgcac aggtaaaaac agagatattc aaattaacag
     2641 aaacacacct gatattnnnn nnnnnnaaag ctgaaaacaa aaagctttct gtttttaaaa
     2701 aaatcttcgg attttttggc gcagtactgt tcatactcgg tctgattgta aatctggaat
     2761 atattccgaa tttttcagcc aaaactttgc ctacctggtt caactgggga attatcggag
     2821 tatctgttgc gttgcttatg attgctttcg gaacaaaatc aaaacgaccg attgacctgc
     2881 cgagaaaaag ttcaaaactg agtaaaagaa ccgtcagtat ggcaattatg gttttgcttg
     2941 caattccagt tacaattttt gttggaatga catatcttca ggatcagaaa tatcttttta
     3001 tttcactcct ggttatgatt gagtgtatga ttccgttttt tctcgttttt gaaggcaggc
     3061 acccaaaagc gagagagctt gtcataattt ccgttctatg tgcaatagca gttgcaggac
     3121 gacttgcatt tacaatgttg cctcagttca agcccgttgt ggcaattgtg ataatttcgg
     3181 gagtagcatt cggcggtgaa tcgggatttc ttgtcggtgc tgtaagtatg cttgtttcaa
     3241 atcttatgtt cggtcaaggt ccttggacgc cgtggcaaat gtttgcggca gggataatag
     3301 gcttcattgg cggtattctc tttaagaaag gtcttttggg cagaacaagg acttcgctct
     3361 gtatatacgg tttcattgcc acaatggtca tttacggcgg acttatgaac ctttattcgg
     3421 cactcacatc acattccgca tttaatctaa atatgctcat aactttttat gttcagggct
     3481 ttccgatgga tattgttcac gcagtttcga ctgttatatt tctgtttttc ggagcagaac
     3541 ctatgcttga aaagcttgac agaattaaag ttaagtacgg acttatcgaa taaacctaaa
     3601 cagcagataa aatttctgct gtttttttgt gctgtaattc aaaaattttc gacaaaagta
     3661 tagacataat ccgaaaataa tattataatt ttggtgtgtc tgattttaat caaacagtat
     3721 ttacaatcta aaacggcaaa ctgtaaatac ttttaattca gaattaaaaa ggttaaagga
     3781 gaaaaaacat gggcggattt atgggtgttg cagcaaaaca ggactgcgta ttcgacttat
     3841 actttggaat tgactatcat tcacaccttg gcacaagaag aggcggtatg gttgtttatg
     3901 acaaagaaaa aggatttgac agatcaatcc ataatattga aaatgcacct ttcagaacta
     3961 aatttgataa agatatgcag agtatgaaag gcaaattcgg tatcggctgt atttccgact
     4021 acgaaccgca gcctcttatt gttcgttcac atcacggtac atatgcaatt accaccgtaa
     4081 gcaaaatcaa taatacagat gaacttgttg aagaaatttt tgaaaaaggc ggttcacatt
     4141 ttcttgaaat gagcggcggt gaaatcaacg caaccgagct tattgcaact ctaatcaatc
     4201 aaaaagaaaa ccttgttgac ggcataagct atgctcttga aaaagttgac ggctctgttt
     4261 cgctcctgtt gatgaataaa aacggcatct attgcgcaag agataagtac ggcagaactc
     4321 ctgttgttat cggcaaaaaa gaagatgcat tttgtgtggc gtttgaatgt ttctcataca
     4381 aaaacctcgg ctacaatgat tataaagaac tcggaccggg tgagattacg gtaattacag
     4441 accaaaactg cattacactc aaaaagcccg gtgataaaat gaaaatctgt acttttctgt
     4501 gggtttacta cggctatcct gcaagctcat atgagggtac aagtgttgag caaatgcgtt
     4561 acaactgcgg tgcaaaaatg gctgaaagag attccgtaaa gcccgatatc gttgcaggtg
     4621 tacccgattc aggcattgct cacgcagtag gctatgcaaa tgcatcggga attccgttct
     4681 caagaccgtt tattaaatat acacctactt ggccgagatc atttatgccg acaattcaga
     4741 gtcagcgtaa tcttattgca aaaatgaagc ttctcgcagt tgaggattta atcagaggaa
     4801 agagccttct tcttattgat gactcaattg taagaggcac acagcttcgt gaaacaacgg
     4861 aatatctttt tgaaagcggt gcaaaagagg ttcatatcag acctgcgtgt cctccgcttg
     4921 tatacggctg taagtatctt aacttctcaa gatcatcttc tgaaatggat ctcatcacaa
     4981 gacgagttat tgaaagactt gagaacggca atgttacaga cgaaattctt caggaataca
     5041 caaatcccga ttctgaaaaa tacgagcaga tggttgatga aatctgtaaa gaactcaagt
     5101 tcacatcctt gcgttttaac aggcttgacg atatgcttga ttcctgcgga attgacaaat
     5161 gcaaactctg tacttattgt tgggacggta aggaataatt aaattttatt tataaaaata
     5221 aggcatttgc aatgagaaat ctcaaagcaa atgccttttg tttttactta gtatgcaata
     5281 atttcgtagc cgtcctcagt tacaagaacc tgaatttccc actgagcaga cattgagtta
     5341 tcttctgtat agatagtcca gccgttatcc tcatcaacga aaatatcagg ttttcctgcg
     5401 ttcaccatag gttcaatggt aaaaatcata ccgggtacca taagcatttc cgtgcctttt
     5461 ttagaaatgt aacttaccca tggatcttcg tggaattcaa gaccgattcc atgacctcct
     5521 acttcacgaa caacagagta acctttactc tttacataat cattgacagc ctgacccata
     5581 tctccgagaa agccccaagg cttaacctgc ttgagtccct cataaactgc attttttgtt
     5641 tcttcaacaa gctttctgtt ttcctcgctt acattaccga tacaaaacat tcttgaagaa
     5701 tcggagaaat atccgttcaa attagttgaa acatcaacat tgattatatc gccgtctttg
     5761 attattacat cttccgacgg aataccgtga caaacttcgt tgtttattga agtacatacg
     5821 ctcttgggaa atccctcaaa attaagggga gccggaacac cgcccatttt ggttgtgata
     5881 tcatacaccc acttatcaat ttcagccgtg ctgatacctg ccttaatatg ctctgcaaca
     5941 taatcaagaa ctgcaatatt aatctttgca ctctccttga ttttttcaat ctgctcagca
     6001 tttttcaaga tttcatggga gggaacaatg tgacccgcac cctcaaattc ctcaattttt
     6061 tcatcaaaat ctatatgaca ttttttatac ttttttccgc tgccacacca gcaaggatca
     6121 tttctgccag gtttttccat tttactaact tcctttcatt tttccaattc ataatccttg
     6181 ataattatac caccaaacaa attttattac aacatctttg tcatatgcaa aaatccaaaa
     6241 gaaaaatctt gtcagaaaaa gttttgtagt atatactgaa taagtaatat ataatctcac
     6301 actcggcata tttttatgca gaggtgtttg tatgaataaa aaaatcccga cattttatgc
     6361 tctctgcttt atactgtttg caatattctg cattattatg gtatctgttt ttttcagact
     6421 taatccgaca aaatctgtta tgaccgaaaa caaaacagag ccgataattg taattgacgc
     6481 aggtcacggc ggcgaagacg ggggagcggt aagcgaaagc ggtgtgcttg aaaaggacat
     6541 caatctttct atagcaaata attcagctga tttattaaaa attttcggat ataaggttgt
     6601 tcaaacaaga acagatgatg tatctcttga taacggagaa gattcggttc attcaaggaa
     6661 agttgccgat ttaaaaaaac ggctcgaaat tttcaattca gacaaaaaca atattgtaat
     6721 cagtattcat caaaacaaat tttctcaaag caaatattac ggaactcaaa ttttttattc
     6781 cccgaataac agtcaaagtc aaaatcttgc ggaatgcatt agaaattccg taaaaaaatg
     6841 cttacagcct gataacgaac ggcaatgcaa aaaggcagac ggctcaatct atcttttaaa
     6901 aaaggctgaa aacccgtccg ttattgtgga atgcggattt atttcaaatc cggatgaact
     6961 tgccaagctg caaagtgacg aatatcaaaa agaaatggca actgcaatca taacaggatt
     7021 tttagattat actcatcaat aaatacagga agtgaaataa atggcttcaa aaatcaaaag
     7081 tgtttacata tgttcggaat gtggatacga atcgcccaaa tggtacggaa aatgcccgtc
     7141 atgcggcgaa tggaacacaa tgaatgaaga aattaaagat acatcaaaac agtcagcaac
     7201 tgcaaaaaca cgctcttttg caacctactc aaaaccatat gccatatcgg atatttcaac
     7261 cgaggatgag cacagatatg acacaggctg cggagaactc aaccgtgtac ttggcggagg
     7321 aattgtaaag ggcagtctta tacttctggg cggtgatccg ggcataggca aatcgaccgt
     7381 tcttatgcag atttgtcagt acctcggcga tactctgaaa attctctaca tatcgggaga
     7441 ggagtcaaaa agacagctca agctccgagc aataaggctg ggtgtaactt cgccgaatct
     7501 gtatgtaatg actgaaaccg atgttgaggt tatttgcgaa cagataaaga atctcaagcc
     7561 cgaccttgtt atgattgact caattcagac gatgaatctt tcagaattaa gctcgtcccc
     7621 cggcagcatt acacaggtac gagaatctgc caatatgctg atgagaaccg caaaaggact
     7681 tgatattcct atgtttatcg taggtcatgt caataaagag ggttcaattg caggtccaaa
     7741 ggtacttgaa catatcgttg acaccgtact gcattttgaa ggtgacaaac agatgagctg
     7801 ccgtattctt cgtgccgtaa aaaacaggta cggctcaaca aatgaaatcg gcgtttttga
     7861 aatgactgat aaagggctta acgaagttgc aaatccgtct gttatgttgc tttcagggcg
     7921 tccgaaaaat gtttcgggaa cttgcgtcac ctgtacaatt gaaggatcaa gaccaattct
     7981 tgccgaaaca cagggacttg ccacacaaag cggatacggc aacgctcgca gaatggttac
     8041 aggatatgat tataacagac tgtcaatggt acttgccgtt cttgaaaaaa gagcaggata
     8101 ctatttttca aactgcgaca cctacataaa tatcgtaggc ggattgcgag ttgacgaacc
     8161 tgcgattgac ctttcaattg ccatggctct tataagcagt ctgaaagata ctccgattga
     8221 cgaaaacgct gttgtattcg gtgaaatcgg tcttgcaggc gaaattcgtg ctgtatcaaa
     8281 cgctcagatg agaattaacg aagcggtaag actcggcttt acaaaaatca ttctgccgta
     8341 tcataatcta aaaggtgttt catgtgatga tgctgaattg atcggtgtaa aaaatattcg
     8401 tcaggcattt gaagcggtag gtcaatgagc agttttgtaa tatcaaacaa atacccatgt
     8461 tttgccgatg agctttcaaa aatggggcat aatgttatat tttcggacac tgtcaaagca
     8521 tttccgcaac ctgaacaagc tcatgccgat atgcagattt tgacgataaa taatactgtt
     8581 tttgtacttc aagagtgcga aaagctaaaa actttaccat ataaagaaaa tttgattata
     8641 tgtaaaagca aagcagggaa gaagtatccc gaaaatatat tgctgaattt tcttttcttt
     8701 aataataagc tttacggtaa agtatctgca atagatccga ctctttacaa atattgcgta
     8761 aaaaacgata ttgaaatcgt aaacataaac caaggatacg caagatgctc aactctaatg
     8821 ctcaataatc gcactgccgt aacagctgat atttccataa aaaatgccct tgaaaaagat
     8881 ggggcagagg tgttattaat atcctcgggt gatataaaac ttgaaggata tgattatgga
     8941 tttataggcg gtgcaagcgg aaaaatctct gataataccg tagtgttttt cggaaatgct
     9001 gaaatgcatc cctgctattc ttccataaga gaattatgtt taaaaaataa aattgaaatt
     9061 aaaatattat gcaaaaatat gccactcaca gatgtgggtg gcatagtaaa aattctttaa
     9121 acttttttct taggttttat ttttgcaagc ggatttgagt cagcggcatt tttcatttgt
     9181 tcggaactca aatgcgtata aatctcagtc gtaccgaggt tttcgtgtcc gagaacctct
     9241 ttaagcactc tgacatcaac tccgccttgc tgatacataa gcgtggcagc ggtatgccta
     9301 agcttatgac aggaatatcc ctgagaatca agtccgattt tctcaagata tttatatacc
     9361 atagcctgaa ttgttttcac agacattctg cggtgattac ggctaataaa caacgctttt
     9421 ttatccttaa cttcatcaat cggacgcacc ttcatatact cattataagc ttcaatgcag
     9481 gcagagttta gataaacaat gcgttcttta ttgcctttac cgacaacacg cattgtaccg
     9541 tcactatgaa tatcgttata gttaagacct gccatttcag acacacgcag tccgcaattg
     9601 aggaagagag tcaaaataca ataatcacgc tgtttgttat ttccgtcaac agcattaaga
     9661 agctcaatgc tttgttcaag agtaagatac tttggcaaag cttttttatt ttttggcgat
     9721 tcaagctgtt ccacgggatt tgtatcaagc aaatgtttat tattggttat gtacttaaaa
     9781 aagcctttca gactggaaca ttttcttgca cgggacgcac tcttattgtt gcggtcacga
     9841 taaagaaaat tcatataatc atatgcaagt tcaagattaa cggttttaat taaatcaagg
     9901 tcaacatcgt caattttaat ttcatcaaac tcagtaccgt caggcacaag accacgcatt
     9961 acttttaaaa atctaaaaaa agttctgaga tcaataaaat attcgtcaac ggttttcttc
    10021 gacatatttt tgattgtctg cttataaatt aaatagtcct tcaaaattgc aggagcttca
    10081 tcacgaaaat caactgccat aaacacacca ccgtaaattt atttcttata atggataact
    10141 cagaaaagtt gaactaaaat ctgtattaaa agaattataa ggctattata acacaaaaaa
    10201 gggcggtgta tattccaatt acgaaaatta tacaaaatga atattttgta taattcgggg
    10261 aacaagtaaa acaagataaa accgaaacat actactaatg ccaaactaat gccaatttac
    10321 agcatttaaa tcacaacagc cgtacagact caaacgaatc tgtacggctg taaattttaa
    10381 aggaatttaa cttttaaatt agcaaacgcc ctgctgaatc attgaatcag caaccttctc
    10441 aaagcctgca atattagcac ctactacata gtcaccttca tgaccatact tcttagctgc
    10501 agcagaaatg ttcttataga tgttaaccat aatgttcttg agcttagcgt caacttcttc
    10561 aaatgtccat gaaagacgct gtgagttctg gctcatctca agagctgatg tagctacacc
    10621 gcctgcatta gcagccttac ccggtgcaaa gagaatgccg gatttctgga gaagctttgt
    10681 agcctcaaga gttgtaggca tgttagcgcc ttcggcaaca gcatagcaac cgttagcgat
    10741 aagtctttta gcgtcttctt cgttaagctc attctgtgta gcacatggaa gtgcaacatc
    10801 acacttaact gaccaatcga atttgccttc gtgatactct gagttaggac gatagttctt
    10861 atactctgta agacgctgtc ttctaacttc cttgatttcc ttaatagcat caaggtcgat
    10921 accttcagca tcataaatcc aacctgttga atcagccatt gtaactacct tagcaccaag
    10981 ctctgtagcc ttttctgttg cgtagatagc aacattacct gcacctgata cgcaaacagt
    11041 agcacccttg atgtccttgc cgttatcctt aagcatagcc tctgtaagat aaagaaggcc
    11101 ataacctgta gcctctgttc ttgcgagtga accgccccag ttgagaccct tacctgtaag
    11161 aacgccttca tatacatttc tgattctctt atactgaccg aacatataac cgatctcacg
    11221 agcacctgta ccgatatcac cggcaggaac atctgtgtca gcaccgatgt gcttacaaag
    11281 ctctgtcata aagctctggc agaatgccat aacttctctg tcgctcttgc ccttaggatc
    11341 aaagtcagag ccacccttac cgccgccgat aggaagacct gtaagtgagt tcttgaaaat
    11401 ctgctcgaaa ccgagaaact taataacgcc gaggtttact gacggatgaa gtcttaaacc
    11461 acccttgtaa ggtccgatag cactgttgaa ctgaacacgg aaaccacggt taacctgaac
    11521 cttgccgttg tcatcaaccc aaggtactct aaacataata actctttcag gctctgtaat
    11581 tctttcgaga ataccattct cttcatactt tggattctgc tcgaaaacgg gctcaagtga
    11641 agtaagaact tcggtagcag cctgaataaa ctcaggctca tttgcgtttt ttgctttcag
    11701 cttttcaagc tgttcactta catatgacat aagtaagtcc tccttaataa aattcgcata
    11761 aattatacgg aaaaaattcc gattatgctg tagcacagta aagcactaca atcaacatat
    11821 taataatact atactttcaa cgattttgca atatcttttg tgagtttctt gcaaattttt
    11881 ttaaaattat tgaaaaaaca gccgatttca ttccttttct gcaattaatt ttacaaatct
    11941 cccaagttta gttgcatttt cgtacatttt acgagctgaa ttttatgaaa atgtattcat
    12001 gaatccaaga atttcatttg cattacaata ttttgtataa ttcatatttg atttaacaat
    12061 taaattttag ttgcaaacgg acatatttta tgataaaatc ctattagtaa tactatttgt
    12121 aatatttttg aaggtgattt atatggacta ctgcaaaaga ttaaaagaat taagagtcaa
    12181 aaacggatac acacaagggc aaattgcatt catgcttcac acgaggcagg aacaatacag
    12241 caaatacgaa aacggaaaac gagaactgcc tatcagacat ttgataacac tttgttgtct
    12301 ttataaaact tcaagtgatt atattctcgg aatagaaaaa tttgtaaaat aaagccgtcg
    12361 tcaataccaa atccgagtat tgacgacggc tttattgcta tataaaattg ccgaattatt
    12421 gaattttttc tttaaacatc atcagaaaac ttacaagaag caatccgcat gaaacccata
    12481 ttatatgcgt tccaaacgat aacgaaagat ttcctcccag acctgctccg actgcaaaga
    12541 aaaatataac accaaaataa tacattgcct gtctgagctg ttcgggcttt ctttcgtgaa
    12601 taaaacctga cagagcagcc gtaccgcttc tcaaattacc tatacacatc gtactggcat
    12661 aggaatatcc tctgactttt ctgaaagtct gaacctgcat tgcacaggaa aacgatacaa
    12721 ttaccgccgc aacggtatcc atactcttcg gcatgaatcc aaccgcaaat aatatgataa
    12781 tttcaaacaa gagtattccc tgtctccaat gtatcttttt gaaatacttg aattttttct
    12841 gtatcaaatc agctacaagg acacctaacg caaatgcaag aataggaaaa agatatttaa
    12901 gcccctctcc ccactttcct gtcataaaat ttgtactcat aagcacaacg ttacccgtct
    12961 gtgcatttgc aaatactccg tcacgggtaa aatatgtata tgcatcctga aatccgcctg
    13021 ataacgcaag aaatatacaa tttctaaatg cctcagacat ctgtcgtgtc ggctcaagtt
    13081 ccttaaaaaa acttcttatc ctaaacacaa tactccactc cgaatctatg cgctaatctg
    13141 cacaacacaa caccgaacac aattaaacga tttg
//
LOCUS       DAOMVE010000035        13156 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1070_length_13156_cov_3.102893, whole genome shotgun sequence.
ACCESSION   DAOMVE010000035 DAOMVE010000000
VERSION     DAOMVE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1070_length_13156_cov_3.102893"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1687)
                     /locus_tag="PLS64_03255"
     CDS             complement(<1..1687)
                     /locus_tag="PLS64_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRL42883.1"
                     /translation="MNDNFENNKNGYDGYSSDNNTADNNYQDIHSSSDSQSASTAENT
                     ESSYEWNTNSTSSVNSGEYRHSYINGNNENASHNPNRYESAYSSNTASGTSSQNYSSD
                     SYGSQYSSNPYSSQQTNNYSYGNPSQNPYGSQSGSPYANQQYSAPKAKKVKVKKPKKP
                     KKPASKGFVAAMLVAAIILGGGAGFGGSMLASSLNNNSSSSVNIKQASANSTSTTSNA
                     ALSGTTSEIVKKTADSVVEIATESVVTGGFAQQYVQQGAGSGVIISEDGYIITNYHVI
                     EGAENITVTLRDGATSYKAKIIGSDEDNDIALLKVDATGLSAATMGNSSDLAVGDYVV
                     AIGNPLGQLGGTVTDGIISALARQVTVEGKSMTLLQHNAQISPGNSGGGLFNSNGELI
                     GIVNAKDSATEVEGIAFAIPINNVLDIIDDLKTYGYVKGKVDLGMELTDITSDDSAFY
                     YGLSNTGCYVLSVTSGSNAEKAGFLRGDMITAVNGTSVSSKSDIETALKDSKVGDTVT
                     FTVTRSGKTANLKLTLAEYSPSGSSSTGNSNTQNKSSQSQGKSSDNSDSDSIWN"
     gene            complement(1959..2423)
                     /locus_tag="PLS64_03260"
     CDS             complement(1959..2423)
                     /locus_tag="PLS64_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42884.1"
                     /translation="MIIKFFDKLHYKLSLSEEHINDIIKSNSKYGADIKREDYSYDVK
                     FTDKGFKMKFVPAKNYGGIYSLSPIFSGKIEEDCGYSDLTVRMIPSPLMVVAFIIFSF
                     LFFIGCNLILTGTLNPRFKGVGFVIIFFCVFIQLTNRFFNNMKSIVDGLFSK"
     gene            complement(2451..3341)
                     /locus_tag="PLS64_03265"
     CDS             complement(2451..3341)
                     /locus_tag="PLS64_03265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL42885.1"
                     /translation="MSYVILDLEWNGSYSKVLHKFVNEIIEIGAVKLDDELNVCDTFT
                     MLVAPKIGKKLCSKVKQLTKITNEELKDDGVSFIKAISLFSDFLGDGVLMTWSDSDLH
                     ALIENYSYYTGRTRLPFLSRYCNLQSYCEDCLDLHDSSCQLGLGACAEMAGVDFSEDD
                     QHRALADVYLTLECMKAFYGKYPLKPYIKDAVCDEFYDRLLFKNHFVTDINSPDVDKS
                     VMFFDCEDCGKPLVQLSKWRLHNKSFTAEFSCKYCRKKFNGRVSFKKKFDEVSVKKKL
                     NEKVEKKPETKEQVNTVSAN"
     gene            complement(3350..5170)
                     /gene="typA"
                     /locus_tag="PLS64_03270"
     CDS             complement(3350..5170)
                     /gene="typA"
                     /locus_tag="PLS64_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRL42886.1"
                     /translation="MDVRENLRNIAIIAHVDHGKTTLVDQLLKQSGVFRANENVEERV
                     MDSNDLERERGITILSKPTAVMYNGIKINIVDTPGHADFGGEVERILQMVDGVVLLVD
                     AFEGCMPQTRFVLKKALGLGKKPLVVLNKIDRPGARPEEVVDEVLDLFIELGADEDQL
                     EFPVIYASARDGYASEDYHEPGTDMKPLFDAIVKEIPAPQGDMNGGLQLLISNIDSDK
                     FVGRIGVGRVERGTAKNGEAAVLCHTDGTTQNVRIAKLYQFEGLKRVECDTATVGDIV
                     CVSGVQDINIGETICSNDCVEPLPFVKIDEPTVSMNFIVNNSPFAGREGKYVTSRNLR
                     DRLFKEIETNVALRVEETDSADTFKVSGRGELHLSILIETMRRQNYEFQVSRPQVITK
                     MIDGKLHEPMEFLIIEVPDTYVGPVMEKLGSRKGELINMGTREGGVTHIEFRIPSRGL
                     MGYRPEFLTDTNGNGIMNHVFDGYEPYKGEIVTRHQGSLIAFETGETVTYGLYAAQER
                     GRLFIGPGVPVYEGMIVGASPKSEDITVNVCKKKHITNTRASGSDDALKLTPPTIMSL
                     EQCLEFIADDELVEVTPENIRMRKRILSKEQRMKQAFRKK"
     gene            5477..>6837
                     /locus_tag="PLS64_03275"
     CDS             5477..>6837
                     /locus_tag="PLS64_03275"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRL42887.1"
                     /translation="MGYTIAQKIIKNHLVCGEMTVGSDIGLKIDQTLTQDATGTMAYI
                     EYEAMGIPRVKTEKSVAYIDHNTLQTGFENADDHRFIQSVCKKHGIYFSRPGNGICHQ
                     VHLERFGKPGKTLIGSDSHTPTGGGIGMLAIGAGGLDVAVAMGGGAYYITMPKMVRVN
                     LTGKLSPWVSAKDIILAVLRVMSVKGGVGKIVEYGGEGVATLTVPERATITNMGAELG
                     ATTSVFPSDETTRKFLKAQGREEDYTELKADDDAVYDEVIEINLSELEPLAACPHSPD
                     NVKTIKELEGKKIDQVCIGSCTNSSYLDLMRVAHILKGKKVADNVSLAIAPGSKQVFN
                     MLALNGALGDMIAAGARILESACGPCIGMGQSPNSGGISLRTFNRNFEGRSGTADGQI
                     YLVSPETAAVSAINGVFTDPRCLGAAAEIEMPEKFLINDNMVIDPAPVEEMDNIEVLR
                     GPN"
     assembly_gap    6838..6847
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6848..7433
                     /locus_tag="PLS64_03280"
     CDS             <6848..7433
                     /locus_tag="PLS64_03280"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523656.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HRL42888.1"
                     /translation="RGPNIKSYPETHPLTDSIEASCSLKVGDNITTDHIMPAGAKILP
                     LRSNIPKISEHCFTVCDKEFPTRAKTLGKSIIVGGENYGQGSSREHAALAPLFLGVKA
                     VLVKSFARIHKSNLINAGILPLTFVNAEDYDKIKLGDMLELPNVKDEIANGKQVTVVN
                     KTTGDTIVADCELSDRTRNIIIAGGLLDYTKENS"
     gene            7435..8643
                     /locus_tag="PLS64_03285"
     CDS             7435..8643
                     /locus_tag="PLS64_03285"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRL42889.1"
                     /translation="MEKIKMVTPLVEMDGDEMTRVIWKQIKDILITPYVDLKTEYYDL
                     GLEHRNETDDQVTVDSANATKKYGVAVKCATITPNAARMTEYNLKSMWKSPNGTIRAI
                     LDGTVFRSPILVKGIVPYIPTWKKPICIARHAYGDVYKNTEMKVEAGSKAELCVTKPD
                     GTEERSTIFDFKTDGIIQGMHNIDKSISSFARSCFNYALDQKLDVWFATKDTISKIYD
                     HRFKDIFSEIFENEYKEKFENAGITYFYTLIDDAVARVIRSEGGYMWACKNYDGDVMS
                     DMIATAFGSLAMMTSVLVSPDGIYEYEAAHGTVQRHYYKYLKGEETSTNSVATLFAWT
                     GALRKRGELDGLDDLMKFADKLEAATIKTIEDGVMTGDLYAISTLENKKKVNTEEFLK
                     AIDERLAASL"
     gene            8672..9304
                     /locus_tag="PLS64_03290"
     CDS             8672..9304
                     /locus_tag="PLS64_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HRL42890.1"
                     /translation="MSKNDSISMSVIRRLPRYYRFLSELNDQNIDKISSTKLAQIMNV
                     TASQVRQDLNCFGGFGQQGYGYSVSQLKEEIKNILGLNNDYKAILIGAGNLGKAVAMH
                     MNFEKLGFELIACFDSNEQKVGSKLNGITVKNESELDDFCNKNHIDTAFLCIPRVCVE
                     NVLDKLYSHGIKNYWNFSHYDINARYNDTMVENVHLSDSLMTLCYRMNNN"
     gene            complement(9444..12623)
                     /gene="pulA"
                     /locus_tag="PLS64_03295"
     CDS             complement(9444..12623)
                     /gene="pulA"
                     /locus_tag="PLS64_03295"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HRL42891.1"
                     /translation="MRTTKKIVSAVLAACMLASTAVVSSFAATADDGSAVSSDYARDN
                     SYTKAAEDIDAQYAYSGNDLGVTYTKDATTFKVWSPTATGVKLNIFTKGSDDEQGASK
                     VASYTLEKMLVDGEWNGVWTITLVGEWKDYYYTYSVTTTDTTHIGSDATKTYETQDVY
                     STATGVNGKRSMIVDLDETDPEGWSNDNHVLLDKSTKSSVWELHIKDFSYDKASGVSD
                     ANRGKYLAFTESGTTLNGEGKVSTCIDYLKELGVTTVQLNPFYDFQSVNEAGDDSQFN
                     WGYDPVNYNVPEGSYSSNPYDGKVRIKECKEMIKALHDAGISVVMDVVYNHTYSTDSC
                     FQYTVPNYYYRMKTTGAFSNGSGCGNEGATERAMYRQYVIDSLKYWVNEYHVDGFRFD
                     LMGLMDVETMNMAREALDQIDPRITMWGEGWAGGDSYHPTNTCSGTKFYPATQANASR
                     LSDRIAIFNDGIRDGIKGSAMEINDVGFIQGSKSSAKGVSYGVRANSSGTYKWKAQAP
                     SQCVTYDACHDNATLYDQIIASTGLADYGERNSEAVKMNRLASAIIYTSQGISFTLAG
                     EEMARSKDGDTNSYKSAADLNMIKWQNVVDYADVVSYYKGMMQIKSAFSPLTAMDNSY
                     ADKYTFTKKVSASTNQISFTIQNDVEGEWNKMAVIYNNATTAADVTLSDTSVTDWVVI
                     ANGETAGLDSLGEVTGSTFTVPARSAIVAVDKAGYESAGIHSSKGKVKVNYVYEATGE
                     KLEDSVILQGSVGSGYVTVPSAVVPDTYIVSRIGGNAEGKYTSDMQEVTYYYTDYIPE
                     SLKNADFNGDGEVNVIDATLLQKYIVKLETPTVDESVLDLNYDGTFNVEDSTMIMKYV
                     VGIPVSSGKVTVNYYYTDADGKQQKLTDSIVFAGRAGSTYKSTAFKVVGYAVDPDRMP
                     ENQSGLIPYGDAEVNYYYIASSLDITLHVKHNGSLTWTPYLWIWGSDLKGKDSGNFMS
                     EWPGDPMTKGENGWFDYGFTYKGAGTYNVIVSDNATNQTIDYKGFVDNEMWIVIDDSA
                     VMGSTYLTFYTDNPDTNPNAPIAEQVTLG"
     gene            complement(12691..>13156)
                     /locus_tag="PLS64_03300"
     CDS             complement(12691..>13156)
                     /locus_tag="PLS64_03300"
                     /inference="COORDINATES: protein motif:HMM:NF018196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HRL42892.1"
                     /translation="PSIKNYKLVLDDLPTNGAGKLDSASKTVTYKYTRVTDDEDKTVC
                     RVNAIYMDDSGKILDTKTITGVEGQAYSLSQNTYEEKDLVSVPEKANGTFKSGEINVV
                     FSYSSNPDPLKQMLPFVYVGTGLIIALCAASVIYSSNKRKKRIMAELDIEED"
BASE COUNT         3677 a         2835 c         2771 g         3863 t
ORIGIN      
        1 agttccagat tgagtcgctg tcactgttat ctgaactttt gccctgtgat tgtgacgact
       61 tattctgtgt gtttgaattg cctgttgaag aactgccgct cggtgaatat tctgcaagag
      121 tgagcttgag atttgcggtc ttaccgcttc ttgtaacggt aaaagtaact gtatctccaa
      181 cctttgaatc cttgagggct gtttcaatgt ctgattttga agaaacactt gtaccgttta
      241 ctgcggtaat catatcgcct ctcagaaatc ctgctttttc tgcattagaa ccgcttgtta
      301 ccgaaagaac ataacagcct gtgttgctga gtccgtagta gaatgcggaa tcgtcggatg
      361 tgatatctgt aagctccata ccgaggtcaa ccttgccctt tacataaccg tatgttttga
      421 gatcgtcaat tatatcaaga acattgttaa tcggaatagc aaatgcaata ccttcaacct
      481 ctgttgcact gtcctttgcg tttacaatac cgataagctc accgtttgag ttaaagagtc
      541 cgccgcctga gttaccgggg ctgatctgag cgttgtgctg gagaagcgtc atactcttac
      601 cctcaaccgt tacctgacgg gcaagtgcgc tgataatacc gtctgttact gtaccgccga
      661 gctgtccgag cggattaccg attgcaacaa catagtcgcc tactgcaaga tctgagctgt
      721 tgcccattgt tgcggctgaa agacctgttg catctacttt aagaagtgcg atatcgttgt
      781 cctcatcact gccgattatc ttagccttgt atgaagttgc accgtcacgg agtgttaccg
      841 tgatattctc cgcaccttcg attacatggt agtttgtgat gatgtaaccg tcctcggaaa
      901 tgataacacc tgaacctgca ccctgctgaa catactgctg agcaaatccg cctgttacaa
      961 cgctttcggt agcaatttca actacgctgt cggctgtttt cttaacaatt tcggaagttg
     1021 ttccgctgag agcggcattg cttgtggttg atgtgctgtt tgcggatgcc tgtttgatgt
     1081 ttacacttga agatgagttg ttgttaagtg atgatgcaag catactgccg ccgaatcctg
     1141 caccgccgcc gagaatgatt gcagctacaa gcattgcggc tacaaaaccc tttgatgcgg
     1201 gtttcttcgg ctttttaggc tttttaactt ttactttctt agcctttggt gcggaatact
     1261 gttgatttgc atacggtgaa cccgactgtg agccgtatgg attctgtgac ggattgccgt
     1321 atgaatagtt gttagtctgc tgtgaagaat atgggtttga agaatactgt gaaccgtatg
     1381 aatccgatga gtagttctgt gaagatgtac ccgatgctgt gtttgaagaa taagcacttt
     1441 cataacggtt agggttgtgt gacgcattct cgttgttacc gtttatatac gaatggcggt
     1501 actcacccga atttacactt gatgttgaat ttgtgttcca ttcataagag ctttctgtgt
     1561 tttctgcagt tgatgcagac tgtgaatctg atgatgaatg aatatcctga tagttgttgt
     1621 cagcagtatt attatctgac gaataaccgt cataaccgtt tttattgttt tcaaagttat
     1681 cgttcataac tgtacctccg attaaaagtt atattttcaa aagacttcat cgaatgtcgg
     1741 tgttgttttt tgatgttgta gatataataa agccccaaag tgaaaagtat atgaaatacg
     1801 gctgaattaa tgttgaaagc aagaaaatag tatgtgaaaa ttcggtgtgc gttgtgagga
     1861 gtacgacaca atcatctgta ttcacgaaaa aagggcagaa caaaacgctc tgccctatat
     1921 aatgtttaag ataataacaa ttttaaatct cagttatcct atttactaaa taatccgtca
     1981 acaatacttt tcatattatt gaagaaccta ttagtaagtt gtatgaacac acaaaagaat
     2041 ataatcacaa agcctacacc tttaaaacga ggattaagcg ttcctgtcaa aataagatta
     2101 caaccgataa agaagagaaa cgaaaagatt atgaaagcaa caaccatcag cggacttgga
     2161 atcattctga cagttaaatc agaatatcca caatcttcct ctatcttacc tgaaaaaatc
     2221 ggactcaggc tataaattcc accgtaattt ttagccggta caaatttcat tttaaagccc
     2281 ttatccgtaa attttacatc ataggagtaa tcctctctct taatgtcagc accatactta
     2341 gaattacttt taattatatc atttatgtgt tcttcggata aagaaagttt ataatgaagt
     2401 ttatcaaaga attttataat catagaaata actccatata caaaaggcac tcagtttgcc
     2461 gaaacggtgt tgacctgttc ttttgtttcg ggcttctttt caaccttttc gttaagcttt
     2521 ttctttaccg aaacttcgtc aaacttcttt ttgaaggata ctctgccgtt gaacttcttt
     2581 ctgcaatatt tgcatgaaaa ttcggcggta aagcttttgt tgtgaagtcg ccactttgaa
     2641 agctgaacaa gcggttttcc gcaatcttca caatcgaaaa acataacgct tttgtcaaca
     2701 tcgggactgt tgatatctgt aacaaaatga tttttgaaca gcagtctgtc atagaattcg
     2761 tcacaaacag catccttaat gtacggcttt aacggatact taccgtaaaa agccttcata
     2821 cattccaatg ttaggtacac atcggcaaga gccctgtgct gatcatcttc cgaaaaatca
     2881 acacctgcca tttcggcaca ggcaccgagt cccagctgac aggatgaatc gtgcaaatca
     2941 agacaatctt cacaatagct ttgcagattg caatagcgtg agagaaacgg aagtctggtt
     3001 ctgcccgtgt agtagctgta gttttcgata agagcatgaa ggtcggagtc gctccatgtc
     3061 ataagcacac cgtcacccag aaaatccgag aacaggctga ttgctttgat aaagctgacg
     3121 ccatcgtcct tcaactcttc atttgttatt ttagtaagct gttttacctt actgcatagc
     3181 tttttgccga ttttcggagc aacaagcatt gtaaaggtgt cgcatacatt cagttcatca
     3241 tcaagcttaa ccgcaccgat ttcaattatt tcattgacaa atttgtgcag aaccttgctg
     3301 tatgaaccgt tccattcaag gtcaagaatt acataactca ttattttaat tatttcttac
     3361 ggaatgcctg tttcatacgc tgttcttttg aaagaatacg cttacgcata cggatatttt
     3421 cgggagtaac ctcaacaagc tcatcatcag caatgaattc aaggcactgt tcaagcgaca
     3481 taattgtcgg aggtgtaagc ttgagggcat catcagaacc cgatgcacgg gtgtttgtga
     3541 tgtgcttttt cttgcagaca ttaactgtga tatcctcaga cttaggactt gcgcctacaa
     3601 tcataccctc gtaaacagga acaccgggtc cgataaagag tctgcctctt tcctgagcgg
     3661 cataaagacc gtaggttaca gtttcgcctg tttcaaaagc gataagtgaa ccctgatggc
     3721 gggttacgat ttcacccttg tatggctcat aaccgtcaaa tacatggttc ataataccgt
     3781 taccgtttgt atctgtaaga aattcggggc gataacccat aagacctctt gacgggattc
     3841 tgaattcaat gtgagtaaca ccgccttcac gagtacccat attgataagc tcaccttttc
     3901 ttgaaccgag cttttccatt acaggaccga catatgtgtc gggaacttcg atgataagaa
     3961 actccatagg ctcatgaagc ttgccgtcaa tcatctttgt aataacctgc ggacgggaaa
     4021 cctgaaattc ataattctga cggcgcattg tttcgataag gattgaaagg tggagttcac
     4081 ctctgcccga taccttgaat gtatcggcgc tgtctgtttc ttcaacacga agtgcaacat
     4141 ttgtttcaat ttccttgaaa agtctgtcac gaaggttacg ggaggttaca tacttaccct
     4201 ctctgcctgc aaacggtgag ttgtttacga taaagttcat tgaaaccgta ggctcgtcaa
     4261 tctttacaaa cggaagcggc tcaacacaat cgtttgaaca gattgtttca ccgatgttga
     4321 tatcctgaac accgcttacg caaacgatgt caccaactgt tgcagtgtca cattcaactc
     4381 ttttaagtcc ttcaaactga tagagctttg caattcttac attctgagtt gtaccgtctg
     4441 tgtgacagag aacggcagcc tctccgttct ttgcagtacc tctttcaaca cggcctacac
     4501 cgattctgcc gacaaactta tcggagtcaa tgtttgaaat aaggagctgg agtcctccgt
     4561 tcatatcacc ctgaggagca gggatttctt ttacaattgc gtcaaagaga ggtttcatat
     4621 ccgtaccggg ttcatgataa tcctctgatg catagccgtc acgggcagac gcataaatta
     4681 cagggaactc aagctgatct tcgtcggcgc cgagttcgat aaagaggtcg agaacctcgt
     4741 caacaacctc ctcgggtctt gcaccgggac ggtcgatttt gttaagaaca acaagcggct
     4801 ttttgccgag gccgagtgcc tttttaagaa caaatcttgt ctgcggcata caaccctcaa
     4861 atgcgtcaac aagcaataca acaccgtcaa ccatctggag aatacgctca acctcaccgc
     4921 cgaaatcggc atgaccgggg gtgtcaacaa tgttaatttt gatgccgttg tacattacgg
     4981 ctgtcggctt tgaaaggatt gtgatacctc tttcacgctc aaggtcatta gagtccatta
     5041 ctctttcttc tacattttca tttgctctga aaacaccgct ctgcttcaaa agctggtcaa
     5101 ccagagtagt tttgccgtgg tcaacatggg cgataattgc aatatttctt aaattctctc
     5161 ttacatccat aaaaatacct cgttttgcta aaatcactta aatcttattg catacatcaa
     5221 gcattataac actataaaag ctaaaattca atttatattt ttcatcacct tttatgctaa
     5281 tatccgacac tttttgaaca tttttccgat agaaaaccgc aaaaaatatt ttttagtgaa
     5341 aaaataacga tttttgctca tttcgtgtga ttttttcttt acaatatctc aagattgtta
     5401 tataattaac atagtgtttt gcattttttt gcaggacctg aattatgata ggcattaatt
     5461 tttaggaggt aatattatgg gctacacgat tgcgcagaaa atcatcaaga atcatcttgt
     5521 ctgcggagaa atgaccgttg gcagtgatat cggtcttaaa atcgaccaga ctcttactca
     5581 ggatgcaaca ggcactatgg cttatatcga atatgaggct atgggcattc cgagagtaaa
     5641 aacagaaaaa agcgttgctt acattgacca caacacactt cagacaggtt ttgaaaacgc
     5701 cgatgaccac agattcattc agtctgtttg taaaaaacac ggtatctatt tttcaagacc
     5761 cggcaacggc atttgtcacc aggttcacct tgaaagattc ggtaaaccgg gaaagacttt
     5821 aatcggttct gacagccaca ctccgacggg tggcggtatc ggtatgctcg caatcggtgc
     5881 aggcggtctt gatgttgctg ttgcaatggg cggcggcgca tactatatta ctatgccgaa
     5941 aatggtaaga gttaatctta cgggcaaact cagtccgtgg gtttctgcaa aagatattat
     6001 tctcgcagtt cttcgtgtta tgagcgttaa gggcggcgtt ggcaagattg ttgagtacgg
     6061 cggagaaggt gttgccacac ttacagttcc cgaaagagca acaatcacaa atatgggtgc
     6121 agagcttggt gctacaactt ctgtattccc gtctgatgaa acaacaagaa agttcctcaa
     6181 ggctcagggc cgtgaggaag attatacaga actcaaagct gatgacgatg cagtttatga
     6241 tgaggttatc gaaatcaacc tttcagagct tgaaccgctt gcagcctgtc cgcattcacc
     6301 cgacaatgtt aagacaatca aagagcttga gggcaagaag attgatcagg tttgtatcgg
     6361 ttcatgtaca aactcaagct accttgacct tatgagagtt gctcacattc tcaagggcaa
     6421 aaaggttgca gacaatgtat cgctcgcaat tgctccgggt tcaaagcagg tatttaatat
     6481 gctcgctctc aacggcgcac tcggtgatat gattgcagca ggtgcaagaa tccttgaaag
     6541 tgcctgcggt ccgtgtatcg gtatgggaca gtcacctaac agcggcggta tctcactcag
     6601 aacattcaac cgtaactttg aaggcagaag cggaacggca gacggtcaga tttatcttgt
     6661 atctccggaa actgcggctg tttctgcaat caacggcgta tttacagacc cgagatgtct
     6721 tggtgcggct gctgagattg aaatgcccga aaaattcctc atcaatgaca atatggttat
     6781 tgatcccgct cctgttgaag aaatggacaa cattgaagta ctaagaggac caaacatnnn
     6841 nnnnnnnccg tggtcctaac atcaagagct atcccgaaac tcatccgctc acagattcta
     6901 ttgaggcatc atgttcactc aaggtcggcg acaacattac aaccgaccat attatgcctg
     6961 caggcgcaaa gattcttccg ctccgttcaa atattccgaa aatttcagag cattgcttta
     7021 cagtatgcga caaggaattc ccaacccgtg caaagacact cggcaaaagc attattgtcg
     7081 gcggtgaaaa ctacggtcag ggttcatccc gtgagcatgc ggctctcgct ccccttttcc
     7141 tcggtgttaa ggctgtactc gtaaagagct ttgcaagaat tcacaagagc aaccttatca
     7201 atgcaggaat tcttccgctt acatttgtaa acgcagaaga ttatgataaa atcaagcttg
     7261 gcgatatgct tgaactcccg aatgttaagg acgaaattgc aaacggcaag caggttactg
     7321 ttgtaaacaa gacaacaggc gacacaattg ttgctgactg tgagctttca gacagaacaa
     7381 gaaacattat cattgcaggc ggtctgcttg actacacaaa ggagaacagc tgatatggaa
     7441 aaaattaaaa tggtcactcc tctcgttgag atggacggcg acgagatgac aagagtcatc
     7501 tggaaacaga ttaaagacat tctcatcaca ccttatgttg acctcaaaac agaatattat
     7561 gacctcggtc ttgagcacag aaacgaaaca gacgatcagg ttacagttga cagcgccaac
     7621 gcaacaaaga aatacggtgt tgccgttaag tgtgccacaa tcacacctaa tgccgcaaga
     7681 atgacagagt acaacctcaa gagtatgtgg aaatcaccta acggtacgat tcgtgctatc
     7741 cttgacggta cggtattcag aagcccgatt cttgttaagg gcattgtccc ctacattcct
     7801 acatggaaaa agccaatctg cattgcccgt cacgcatacg gtgatgttta taagaacaca
     7861 gagatgaagg ttgaggcagg aagcaaggca gagctttgcg ttacaaaacc tgacggtacc
     7921 gaggaaagaa gcacaatctt tgacttcaag actgacggta ttattcaggg tatgcacaac
     7981 attgacaaga gtatctcatc atttgcccgt tcatgcttta actatgcact tgatcagaag
     8041 cttgatgttt ggtttgctac aaaggataca atcagcaaga tttatgacca cagattcaag
     8101 gacatcttca gtgaaatctt tgagaatgag tacaaagaga agtttgagaa cgcaggtatt
     8161 acatatttct acacacttat tgatgacgct gttgcaagag ttatcagaag tgaaggcggt
     8221 tatatgtggg cttgtaagaa ctatgacggt gatgttatga gtgatatgat tgcaactgca
     8281 ttcggttcgc tcgcaatgat gacaagcgtg cttgtttctc ccgacggcat ctatgaatac
     8341 gaggctgctc acggcacagt tcagcgccac tactacaagt atctcaaggg cgaagaaact
     8401 tccacaaact ctgttgcaac attgtttgca tggacaggcg ctctccgcaa aagaggcgag
     8461 cttgacggtc ttgacgatct tatgaagttt gcagacaagc ttgaggcagc aacaatcaag
     8521 acaattgagg acggcgttat gacaggcgat ctctacgcta tctcgacact tgagaacaaa
     8581 aagaaagtaa acacagaaga atttctcaag gctattgacg agcgccttgc agcatctctc
     8641 tgattttaaa gttaaaggag catagtgagt aatgtctaag aatgacagta tttcaatgtc
     8701 ggttatcagg cgtttgccaa gatattaccg ctttttatca gaactgaatg accagaacat
     8761 tgacaaaatt tcttcaacta agcttgcaca aattatgaat gtgaccgcct cacaggtaag
     8821 acaggatctt aactgcttcg gcggattcgg tcagcagggt tacggttaca gcgtaagtca
     8881 gctgaaagag gaaatcaaga acattctggg acttaacaac gattacaagg ccattcttat
     8941 cggtgcgggt aatcttggta aggccgttgc aatgcatatg aattttgaaa aactcggttt
     9001 tgagcttatc gcttgttttg acagcaacga acaaaaagtc ggttcaaagc tcaacggaat
     9061 tacagttaaa aacgaaagtg aactcgacga tttttgtaat aaaaatcata ttgacacagc
     9121 gtttttgtgc attccccgtg tttgtgttga aaatgttctc gataagcttt attcacacgg
     9181 aatcaagaat tactggaatt tcagccacta tgatataaat gctcgttata atgatactat
     9241 ggtagagaat gttcatctca gcgacagcct tatgactctc tgctacagaa tgaacaacaa
     9301 ctgataaacc aatgactccc cgacagtttt ataaactgtc ggggagtttt ccgttacaga
     9361 gcaataataa ccttttttag cgattaaaag ggctgaccgt ttaaggtcag ccctcttttg
     9421 caatttgtgt ttgtgatttc agattaaccg agtgtaacct gctcagcgat tggagcgttg
     9481 gggttagtgt cagggttatc tgtgtagaat gtaaggtatg tactgcccat aacagcagag
     9541 tcatcaatta caatccacat ttcgttatct acaaagccct tgtagtcaat tgtttggtta
     9601 gtagcgttat cggatacaat tacattgtat gtacctgctc ccttatatgt gaagccgtaa
     9661 tcaaaccaac cgttttcacc tttagtcata ggatcacccg gccactctga cataaagttg
     9721 cctgagtctt tgcccttgag gtctgaaccc catatccaga gatatggcgt ccatgtaagt
     9781 gaaccgttgt gctttacgtg aagtgtgatg tcaaggcttg aagcgatgta gtagtagttt
     9841 acctcagcat caccgtaagg aattaagcca gactgatttt ccggcattct gtccggatca
     9901 acagcgtagc caacaacctt gaatgcagtt gacttatatg tgctgcctgc tctgcctgcg
     9961 aatacgattg aatcagtgag cttctgctgt tttccgtcag cgtctgtgta gtagtagttt
    10021 actgttactt taccggaaga tacaggaatt ccaacaacat acttcattat cattgttgaa
    10081 tcttcaacgt tgaaagtacc gtcatagttt aggtcaagaa ctgattcatc aactgtagga
    10141 gtttcaagct ttacaatata cttctgaaga agtgtagcgt caattacatt aacttcaccg
    10201 tcaccgttga agtcagcgtt cttgagtgat tcaggaatgt agtctgtgta gtagtatgta
    10261 acctcctgca tatcggaagt atatttgcct tctgcatttc cgccaatacg gcttacaatg
    10321 tatgtatcgg gaacaactgc actcggaact gttacataac ctgagcctac agaaccctga
    10381 aggattacag aatcttcaag cttttcgcct gttgcttcgt aaacatagtt tacttttacc
    10441 ttaccttttg aagaatggat gcctgctgac tcatagcctg ccttgtcaac agctacgata
    10501 gccgaacgag caggtactgt gaatgttgag cctgtcacct cgccgagtga gtcaagacct
    10561 gctgtttcac cgttagcgat aacaacccaa tctgttacgg atgtatccga aagagttaca
    10621 tctgcagctg tggttgcatt gttataaata acagccatct tgttccattc accctcaaca
    10681 tcgttctgaa ttgtgaatga aatctgattt gttgaagcag aaaccttttt tgtgaatgtg
    10741 tatttgtcag catatgaatt atccatagct gtgagaggcg agaatgctga cttaatctgc
    10801 atcataccct tgtagtaaga aactacatcg gcatagtcaa caacattctg ccatttaatc
    10861 atattaaggt cggctgccga cttgtagctg tttgtgtcac cgtcttttga acgagccatt
    10921 tcttcaccgg caagtgtgaa tgaaataccc tgtgatgtgt agataattgc acttgcaagc
    10981 ctgttcatct taacagcctc ggaatttctc tctccgtagt ctgccagacc tgttgacgca
    11041 ataatctgat cataaagtgt tgcgttatcg tgacacgcat cataggttac gcactgtgaa
    11101 ggagcctgag ccttccactt gtatgtacct gagctgttgg cacgaacacc gtagcttaca
    11161 ccctttgcgg atgactttga gccctgtata aaaccaacat cgttgatttc cattgcactt
    11221 ccctttatac cgtcacggat gccgtcattg aaaatagcga ttctgtcgct gagtcttgac
    11281 gcattagcct gtgttgcagg atagaatttt gtgcctgagc aagtgttagt cgggtggtag
    11341 gagtcaccgc ctgcccagcc ttcaccccac attgtgattc gtgggtcaat ttggtcaagc
    11401 gcttctcttg ccatattcat tgtttcaaca tccataagac ccataaggtc aaaacggaag
    11461 ccgtcaacat ggtattcgtt tacccaatac ttgagtgaat caataacata ctgtctgtac
    11521 attgcacgct ctgttgcacc ttcgttgccg caacccgaac cgtttgagaa tgcgcctgtt
    11581 gttttcattc tgtagtagta gttaggaact gtgtactgga agcatgaatc agttgaataa
    11641 gtatggttgt aaacaacatc cattacaacg gaaatgccgg cgtcatggag agccttgatc
    11701 atttccttgc actctttgat tcttacttta ccgtcatacg gatttgaaga atacgagccc
    11761 tccggaacat tgtagtttac cggatcataa ccccagttaa actgagaatc atcgcctgcc
    11821 tcatttactg actggaagtc atagaacggg ttaagctgta ctgttgttac accaagttcc
    11881 ttgaggtaat caatacaggt tgaaaccttg ccctcgccgt taagagttgt acctgattct
    11941 gtgaaagcaa ggtacttgcc tctgttagca tcgcttacgc ccgaggcctt gtcataagaa
    12001 aagtccttga tatggagttc ccatactgat gactttgttg atttgtcaag aagaacatgg
    12061 ttatcgtttg accaaccctc aggatctgtc tcgtcaagat caacaatcat tgagcgcttg
    12121 ccgtttacgc ctgttgccgt tgagtataca tcctgtgttt cataagtctt tgttgcatct
    12181 gaaccgatgt gtgttgtgtc tgttgttgtg actgagtatg tgtaataata atccttccat
    12241 tcgccgacga gtgtaatcgt ccaaacaccg ttccactcac cgtcaacgag cattttttca
    12301 agtgtgtagc tggcaacttt agatgcaccc tgctcgtcat ccgaaccttt tgtaaagata
    12361 ttgagcttaa caccggttgc tgtcggtgac caaaccttga atgttgtagc atcttttgtg
    12421 taagtaacac caagatcatt gccggaataa gcatactgag cgtcaatatc ttcagcagcc
    12481 tttgtgtaag aattgtctct tgcgtagtcg cttgaaactg ccgaaccgtc atcggctgtt
    12541 gcggcaaagc ttgacacaac tgctgttgag gcaagcatgc acgctgcaag aactgcagaa
    12601 acaattttct tcgtagttct catagataat attcctcctt attaattctt ataatctatt
    12661 ccgtcagcaa acggaaataa ctacctttaa ttaatcttct tcaatgtcaa gctctgccat
    12721 aatgcgtttt ttacgcttgt ttgaagaata aataaccgat gccgcacaca gagcaattat
    12781 aagaccggtg ccgacataca caaacggaag catctgcttg agcggatcgg ggtttgacga
    12841 gtaactgaac actacattga tttcgcccga tttaaatgtt ccgtttgcct tttcgggaac
    12901 gcttacaaga tctttttcct catatgtatt ttgagagagt gaataagcct gtccttcaac
    12961 acctgtgatt gtctttgtat caagaatttt acctgaatcg tccatataaa tggcattgac
    13021 tctgcaaaca gtcttatcct catcatcagt tacacgggta tatttgtatg taacggtctt
    13081 gcttgcggaa tcaagcttgc ctgcaccgtt tgtcgggagg tcgtcaagaa caagcttgta
    13141 atttttaatt gacgga
//
LOCUS       DAOMVE010000036        12977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1096_length_12977_cov_3.885544, whole genome shotgun sequence.
ACCESSION   DAOMVE010000036 DAOMVE010000000
VERSION     DAOMVE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12977
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1096_length_12977_cov_3.885544"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1489)
                     /locus_tag="PLS64_03305"
     CDS             complement(<1..1489)
                     /locus_tag="PLS64_03305"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRL42893.1"
                     /translation="MANKWVYLFTEGNANMRELLGGKGANLAEMTGLGLPVPQGFTIT
                     TEACTQYYEDGREINAEIQAQINEYIEKMEEITGKKFGDKENPLLVSVRSGARASMPG
                     MMDTILNLGLNEDVVEVIAKKSNNPRWAWDCYRRFIQMYSDVVMEVGKKYFEELIDKM
                     KAERGVTYDVELTADDLKELAGQFKAEYKEKIGQDFPDDPKEQLMGAVKAVFRSWDNP
                     RANVYRRDNDIPYSWGTAVNVQSMAFGNMGDDCGTGVAFTRDPATGEKKLMGEFLINA
                     QGEDVVAGVRTPMPIAKMAEEFPEAFEQFQNVCQTLENHYRDMQDMEFTVENKKLYML
                     QTRNGKRTAQAALKIACDLVDEGMRTEEEAVAMIDPRNLDTLLHPQFDAAALKAATPI
                     GKGLGASPGAACGKIVFTAEDAENWNAKGEKVVLVRLETSPEDITGMKASQGILTVRG
                     GMTSHAAVVARGMGTCCVSGCGDIAMDEANKKFTLAGKEFHEGDYI"
     gene            1820..2569
                     /locus_tag="PLS64_03310"
     CDS             1820..2569
                     /locus_tag="PLS64_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRL42894.1"
                     /translation="MKNCAVILAGGEGKRMKSDKPKTLSEVLGRPMLCWVMSALRKAG
                     IDDICVVKGFKKECVEEYLSTLDFSVESVFQAERLGTGHAVMMAKDFLKKHSGNVVIL
                     NGDAPFMDSKTIEDSLKAHIENDCAATVISAKVDDPTGYGRIVRDENGNLRAIVEQKD
                     ADEETLKINEVNSGGFWFDCQLLLSVLDRIKSDNSAKEYYLPDAIKLLLEDGRKVGAF
                     TAQCSDTVLGANDPAQLEQLNEIARAKGYSC"
     gene            2632..3591
                     /locus_tag="PLS64_03315"
     CDS             2632..3591
                     /locus_tag="PLS64_03315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRL42895.1"
                     /translation="MNFHGKDIKIFTGSSNVDVAQGIAGCLGLPLGKSDCTQFSDGEI
                     SVSLHESVRGSDCFIVQSTCAPVNDNLMEMLIMIDAMKRASAARITVVMPYFGYARQD
                     RKAKARDPISAKLCADIITCAGADRVLTMDLHANQIQGFFNIPVDHLQGASLLANHMR
                     EKIGGNNDDYIVVSPDLGSVTRSRNFASKIGTGLAIIDKRRPKANVCEVMNIIGDARG
                     KKVILVDDMIDTAGTLCNAANAIVEKGGATEVYACATHAVLSGPAIERIQNSAIKEVV
                     LLDTIPVPKEKMIDKFTILPVAPTFAEAIARIYNDKPFTAAFG"
     gene            3710..>4851
                     /locus_tag="PLS64_03320"
     CDS             3710..>4851
                     /locus_tag="PLS64_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42896.1"
                     /translation="MFGIVYGSDSDNKIMNRFVDEYELWCNEGKNPDIASIDLDFALD
                     LQKKRLDEKGVKIQTAFTDKETVKNEVPINACTAYDMGECKSNIASKTYEVTEKYFKD
                     GKKKKKIKDRFFFYTMITRLENQNSEVACSCPNCGAVSSVRELLNGCKNCKTRFIMDD
                     LFPKVTNFYFIKTYSLANKSTKKVLAPYLLGGIVAVAAFTVWVVVKDGTFDPATANMV
                     YEIGIRAIPVLLGGLLAGYLAWALKTLFGLFVGAAKSIPMIGPHFNCQKRLPWLMKEV
                     NPNFSYEYFIGKVLALLKIMIFSDDYTNLAVYEGNPMKNPFGDIVDIKYRGVSKLNSF
                     NVTNGCCYVDMTVYATTVKNKNSSFRVKNEKFRLTVCRNVNAMDDGV"
     assembly_gap    4852..4861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5047..5640
                     /gene="pth"
                     /locus_tag="PLS64_03325"
     CDS             5047..5640
                     /gene="pth"
                     /locus_tag="PLS64_03325"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRL42897.1"
                     /translation="MFGKNKSTGSIEYIVVGLGNPDRKYENTRHNAGWLAIDRIADKL
                     GCDVNKIKYKSFIGTCTVNGAKVMLMKPTTYMNNSGQAVVEAMNFYKIPPENVIVIFD
                     DISLDVGKMRIRSKGSDGGQRGMRSIIYLSGSDKFPRIKVGIGAKPNPDWDLADWVLS
                     KFTPDEMKKLDEIFDNAEKAIELIIDGKTDRAMNLFN"
     gene            5655..>8648
                     /gene="mfd"
                     /locus_tag="PLS64_03330"
     CDS             5655..>8648
                     /gene="mfd"
                     /locus_tag="PLS64_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRL42898.1"
                     /translation="MNNLNFLVDVLKESPVFKTLEKNVKPGRTVCASGLSTINKSNII
                     YALCRLKGVTAFCLASDEKEAQTLCNDLSCMGLRAYVYPVRDFNFLDFQSRSHEYEHA
                     RLKVLLKLINHECDVAIACVDAAAQLTVPRNVLEQSVIEFEEGRELSLEKATKALTLL
                     GYERFDAVEGSGQFSLRGGILDFFMPDSDYPVRCEFWGDEITDLSYFDIETQRRFKKA
                     DKITLSPSTEIVIEDRAALADKIEHKAKLLRSKNSAKAKEKLFSEAELIRSGAMIANA
                     DKFINQIYDKPESLFDYLDRNTLVFASEFTAIQERGKSMDFISNETLMQGFEDGTLCR
                     GFDRFALTFNECTEFLQSHGTIVLENFVHGSMPIKLSEIISFSTKQLSAWGGSYKQLK
                     EDVDGLFTPESKGVIFAGTERAAKNLCDTFNADGINAVYSEGADKISKGELLVMQGAL
                     SAGFEYPSQKFFAITYSQVSYRPEKSKKKKKKTGQEIYSLSELAPGDYVVHNVHGIGV
                     FGGIRKIDTHGVIKDYIKIDYAKGDVLYVPVTQLDMVAKYIGPKEDSRVKLNRLGSGD
                     WQKAKARVKTSVKDIAKELIELYSQRMKAKGYAFSADNEWQRDFELSFEYDETPDQLR
                     CCEEIKHDMMRSSPMDRLLCGDVGFGKTEVALRAAFKCIADSKQCALLCPTTILAWQH
                     YQTVIKRFEGYPVRVELLSRFRTAKQQKEILQKLKRGEIDMIVGTHRLVQKDVEFRDL
                     GLAIIDEEQRFGVAQKERFKELCKNVDVLTLSATPIPRTLNMAMSGLRDMSVIEEAPT
                     NRQPVQTYVLEHDDAVINEAIRRELRRGGQVFYLHNNVETISSCAAKIQEAVPEAKIA
                     IGHGKMKEGELSEVWRQMLEQEIDVLVCTTIIETGVDLPNANTLIIENADCMGLSQLH
                     QLRGRVGRSSRRAYAYFTFRRNKVLTEIQQKRLAAIREFTEFGSGFRIAMRDLELRGA
                     GNIMGAQQHGHMESVGYDMYLKLLDE"
     assembly_gap    8649..8658
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8659..9122
                     /locus_tag="PLS64_03335"
     CDS             <8659..9122
                     /locus_tag="PLS64_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRL42899.1"
                     /translation="VSEEKGEQNVTKDLDCLIDISVDAHIPESYVESLTLRLDVYRRI
                     ADIRSNEDADDVIDELQDRFGKIPNSVLGLIDIALVRNKAYSMGIYEIRQNGTTLMLF
                     VNELKSPQVADLLIALGGKAKLVAGAKPCVAVECKNSSAPLEILTKIFGVQ"
     gene            9328..10524
                     /locus_tag="PLS64_03340"
     CDS             9328..10524
                     /locus_tag="PLS64_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42900.1"
                     /translation="MKPNIKVGSFLLAIVMMFSVFAIAGCTPTTLNKEWSYKTSDNEL
                     AIGVYIYSLNAAYSQAESFAKKLDDYDSTSDKWLDEKIKDDDGNEQVAREWIKDQAKK
                     MCLSYLVVDEQLKKENVNIGQATLDSATSQAESYWNVGPYASQGYVMPMKKQYEKYGV
                     SLDSFAYCTTLYNTKYEALFKAVYGKGGSKEVSDADLTKYFKENYTDYSYLPVNLYTS
                     TKDEAGSSKNVAMSDKEIKKVEDQLNGYKNDLNKGGSFDDVIASYKKSSGSGTDSSVS
                     NVEVLDKSSIGDELKKAIGKLETGKAETLKVGSGDSAIYYLVYKKDVSKDVDSYIGNE
                     SNRAGVLASMKSDEFSKYIDSLAEKLKYEENTSVIDKYKPELFFVAVEPTTSASTTSA
                     SDKSSK"
     gene            10582..11451
                     /locus_tag="PLS64_03345"
     CDS             10582..11451
                     /locus_tag="PLS64_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42901.1"
                     /translation="MKKHSKLCAIFLTALLSISAVSFSVSAVGEEVDPGGDSSVIVDP
                     VEPEPEPEPEPEPSPEPEPQPQPDPVYPDSSDSSSYVDPGYSSSDSSYDNNYNYDSSY
                     DYNNNSYDNNYSSSQTYYYSEPSYVGGGQSYVAPSNTAPSASLIKSDDNIDEKTLSSN
                     DWSDIAKNLKNTDTSSSDGDDFNFIKNNNSKGDNGHWMFYVSLVLLSLGVAGIAYFVI
                     STVLNRKKRVTVGGRSNAPKSKSAQPQRYSSNDDYADDYSSSGRKSERRSKYDTAEIQ
                     LPKNSQKGGRRYK"
     gene            complement(11553..11777)
                     /locus_tag="PLS64_03350"
     CDS             complement(11553..11777)
                     /locus_tag="PLS64_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007051012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HRL42902.1"
                     /translation="MDKKMLDRINELAKKKKEQGLTEAEQKEQKELYKIYLGEIRTQF
                     NATLDNVSVEEKDGTVKPFKEAYKKPYDKN"
     gene            11949..>12977
                     /locus_tag="PLS64_03355"
     CDS             11949..>12977
                     /locus_tag="PLS64_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42903.1"
                     /translation="MENFSLLHPTNSNASYRTFNKEALNDLSVDYICDALTKEQYERN
                     SIKQLLINITDDEEVIKYRCDVFEDFLRFPKLRDDLSALLVKLNDLREIERFQKDTEA
                     SSLWQLVNRLREMDGYVDCITRIKNTLETIEVKSQGLLELKKNVQSIYNDSGFPELKK
                     DIIDTLAKAQKLKSITLGVNLDDLLRPKSVGVISLNDKEFTDSGILKRFMKFTNRDSE
                     LHHGNDVSGFLSFHPSNPSTSQFGLGQFVVGAQQDVNHTMNSSLTGADPMSDALKNVV
                     TDILRRTVNDIKSMLNRYVNVNGYSFVSLMPEIIFYIRFAELCDKMKKKNLPLCKAEV
                     LPKEDRNCC"
BASE COUNT         3764 a         2785 c         2958 g         3450 t
ORIGIN      
        1 aaatgtagtc accctcgtgg aattccttgc ctgcgagtgt gaacttcttg ttagcttcgt
       61 ccattgcgat gtcgccgcaa cctgatacac agcatgtacc cataccacga gcaacaacgg
      121 cagcgtgtga tgtcataccg ccacgaactg tgaggatacc ctgtgaagcc ttcatacctg
      181 tgatgtcctc aggtgaagtt tcaagacgaa cgagaacaac cttttcgcct ttagcgttcc
      241 agttttcagc atcttcggct gtgaatacga tcttaccgca agcagcaccg ggagaagcgc
      301 cgaggccctt accgattggt gtagcagcct taagagcagc agcatcgaac tgtgggtgaa
      361 gaagtgtgtc aaggttacga ggatcgatca ttgcaacagc ttcttcttct gttctcatgc
      421 cctcgtcaac gaggtcacaa gcaattttaa gagcagcctg agctgttctc ttaccgtttc
      481 ttgtctggag catatagagc ttcttgtttt caacagtgaa ctccatgtcc tgcatatctc
      541 tgtagtggtt ttcgagtgtc tgacaaacat tctggaactg ctcgaaagct tccgggaact
      601 cttcagccat cttagcgatt ggcataggag tacgaacacc tgcaactacg tcttcgccct
      661 gtgcgttgat gaggaactca cccatgagct tcttctcacc tgtagcagga tctcttgtaa
      721 atgcaacacc tgtaccgcaa tcgtcaccca tgttaccgaa tgccattgac tgtacgttaa
      781 cggcagtacc ccatgaataa gggatatcgt tgtcacgacg gtaaacgttt gcacgagggt
      841 tgtcccatga acggaataca gccttaactg cgcccatgag ctgttcctta ggatcgtcag
      901 ggaagtcctg accgattttc tctttgtatt cagccttaaa ctggcctgca agctccttaa
      961 gatcgtcagc tgtaagctct acgtcataag taacgcctct ctcagccttc atcttgtcga
     1021 tgagctcttc aaaatatttc ttacctactt ccataactac atcggaatac atctgaatga
     1081 atcttctgta gcagtcccaa gcccaacgag ggttgtttga cttcttagcg ataacttcaa
     1141 caacatcttc gttaagaccg aggttaagga ttgtatccat catacccggc attgaagcac
     1201 gagcacctga acgaactgaa acgaggaggg ggttctcctt gtcgccgaac ttcttgcctg
     1261 tgatctcttc catcttctcg atgtactcgt tgatctgagc ctgaatttca gcgttgattt
     1321 ctctgccgtc ctcataatac tgagtacaag cttctgttgt aattgtgaag ccctgtggta
     1381 caggaagtcc aagacctgtc atttctgcaa ggttggcacc cttaccgccg aggagctcac
     1441 gcatgttagc attaccttct gtaaaaaggt aaacccattt gtttgccata actggtaatc
     1501 ctcctaaaaa taaattaaga tatacggctg tggcagactt accccttggt aaaacctgcc
     1561 tgcttgtata agcctatcga atattataac aaactttggt caaaatagct atattttttt
     1621 caaacttttt tcttataaaa aacgagtttt attttcggca atattgtata aatcgtttaa
     1681 aatgcctttt tttgcccgat ttttgtaatt ttctcttgaa atttgtgatt tttgtgagat
     1741 aatattatgg tatgaacgag tgccttgtga caggactgtc tgcatctttg ttgttttgtc
     1801 acagataaaa ggagaaaaaa tgaagaattg tgccgttatc ctcgcaggcg gcgagggcaa
     1861 aagaatgaaa tccgacaaac cgaaaacatt gtcggaggtg ctcggcagac cgatgctctg
     1921 ctgggttatg tcggctttaa ggaaagccgg tattgacgac atctgtgttg tcaagggctt
     1981 taaaaaagag tgtgtcgaag agtatctttc aaccctcgat ttttccgttg aaagcgtgtt
     2041 tcaggctgaa agactcggaa caggtcacgc tgtgatgatg gcaaaggatt tccttaaaaa
     2101 gcacagcggc aatgttgtta ttctcaacgg cgacgctccg tttatggaca gcaagactat
     2161 tgaggattcc ctgaaggctc atattgagaa cgattgtgcc gcaacggtta tttccgcaaa
     2221 ggttgacgat ccgacaggtt acggcagaat tgtccgtgac gaaaacggaa atctgcgtgc
     2281 aattgttgag cagaaagacg ccgatgagga aactctcaaa ataaatgaag taaactcggg
     2341 cggattctgg tttgactgtc agcttcttct ttcggttctc gacagaatca agtcggataa
     2401 cagcgcaaag gaatattatc ttcccgacgc aatcaagctt ttgcttgagg acggaagaaa
     2461 ggtcggagca ttcacggctc agtgcagtga tacggttctg ggtgcaaatg atccggctca
     2521 gcttgaacag cttaacgaaa ttgcaagagc aaagggttac agctgttaat tgctcggtta
     2581 atcattggta aacatatact acacacatta aaattcaaag gagttattgc aatgaatttt
     2641 cacggtaagg acattaaaat tttcaccggc agttcaaatg ttgatgtagc tcagggaatt
     2701 gcaggctgtc tcggtctgcc gcttggtaag agtgactgta cacagttctc agacggcgaa
     2761 atctctgttt ctcttcacga atctgttcgt ggcagcgact gctttattgt tcagtcaacc
     2821 tgtgctcccg tcaacgacaa tcttatggaa atgctcataa tgattgatgc catgaagcgt
     2881 gcatctgctg caagaatcac agttgttatg ccatacttcg gctatgcaag acaggacaga
     2941 aaagctaagg caagagatcc gatctcggct aagctttgtg cagacatcat cacctgtgca
     3001 ggtgctgacc gtgtgcttac aatggatctt cacgcaaatc agattcaggg cttcttcaac
     3061 attcctgttg accaccttca gggtgcatca cttctcgcaa accatatgag agagaaaatc
     3121 ggtggcaaca atgatgatta tatcgttgtt tctcctgacc tcggttctgt aactcgttca
     3181 agaaacttcg cttcaaaaat cggcacaggt cttgctatta tcgacaagag aagaccaaag
     3241 gctaatgttt gtgaagttat gaacatcatc ggtgacgcta ggggtaagaa agttatcctc
     3301 gttgacgata tgatcgacac agcaggtact ctctgcaatg ccgctaacgc tattgttgaa
     3361 aagggcggtg caacagaggt ttacgcttgc gctactcacg cagttctttc aggtcctgca
     3421 atcgagagaa tccagaacag cgcaatcaaa gaagttgttc ttcttgacac aattcctgtt
     3481 ccgaaggaaa agatgattga caagttcaca attcttcctg ttgctcctac atttgcagag
     3541 gctatcgcaa gaatctacaa cgacaagccg ttcacagccg ctttcggttg ataggcgaac
     3601 aatttaatta tgtaattaaa caggcgggtt ttaactaacc cgccatttcg ctgttttact
     3661 gcaaattttt caggaataaa acggtacaat atgtgagggt gatttaattt tgtttggtat
     3721 agtttacggc tccgattcag ataacaaaat aatgaaccgc tttgtcgatg agtacgaatt
     3781 gtggtgcaat gagggcaaaa atccggatat tgcgtcaatc gacctcgact ttgcgcttga
     3841 cctacagaag aaaagacttg acgaaaaggg tgtaaagatt cagaccgctt ttaccgataa
     3901 ggaaacggtg aagaatgaag tacccataaa tgcttgtact gcctacgata tgggcgaatg
     3961 taagagtaac attgcgtcaa agacatatga ggttacggag aaatacttta aagacggaaa
     4021 gaaaaagaaa aaaatcaagg acagattttt cttttacaca atgattacaa ggcttgaaaa
     4081 ccaaaattcc gaggttgcgt gttcgtgtcc gaactgcggt gcggtaagca gtgtaagaga
     4141 gcttctcaac ggttgtaaaa actgcaaaac tcgctttatt atggacgatt tgttcccgaa
     4201 ggttacaaac ttctacttta taaaaaccta ttcgcttgcg aacaagtcaa cgaaaaaggt
     4261 gcttgctccg taccttctcg gcggaattgt cgccgttgcg gcatttacgg tatgggtggt
     4321 tgtaaaggac ggaacttttg atcccgcaac cgcaaatatg gtctatgaaa tcggcataag
     4381 agcaatcccc gtactgctcg gcggactttt ggcggggtac cttgcatggg cattaaaaac
     4441 gcttttcgga ctttttgtcg gtgcggcaaa gagcattccg atgataggtc cgcacttcaa
     4501 ttgtcagaaa agacttccgt ggcttatgaa agaggttaat cccaacttct catatgaata
     4561 ctttatcggc aaggtgcttg ctctgctcaa gattatgatt ttcagcgatg attacacaaa
     4621 cctcgcagtc tatgagggca atccgatgaa aaatcctttc ggagatattg ttgacatcaa
     4681 gtacagagga gtgtcaaagc ttaattcatt caatgtcaca aacggctgtt gctatgttga
     4741 tatgaccgtt tacgcaacga ccgtgaaaaa caaaaattct tcatttaggg ttaaaaacga
     4801 aaagttcagg cttaccgttt gcagaaatgt gaacgccatg gatgacggag tnnnnnnnnn
     4861 nttttcacaa tgaaaaaagt aacctgtaaa agctgcggcg caagctttga tgcaacaaga
     4921 gaacgcaact gcccgtattg cggaaatcct tatcatctcg gcaacgatga ctgggttgtt
     4981 gtaagcttcg gaaaaggtta atttaaattt cagaatacat ataatataaa taagaggaaa
     5041 acggatatgt tcggaaaaaa caaatcaacg ggttcaatcg agtacattgt agtgggactc
     5101 ggcaaccccg acagaaaata cgaaaacact cgtcacaacg caggctggct tgcaattgac
     5161 cgcattgccg acaagctcgg ttgtgatgtg aacaagataa aatacaaaag ctttatcggc
     5221 acttgcactg taaacggcgc aaaagttatg ctgatgaaac ccacaacata tatgaacaac
     5281 agcggtcagg cagttgttga ggcgatgaac ttctacaaaa ttcctcccga aaatgtcatt
     5341 gtaatttttg atgacatttc gcttgatgtc ggaaaaatgc gtatccgttc aaagggcagt
     5401 gacggcggtc aaaggggaat gcgcagtata atctatctca gcggaagcga taaattcccc
     5461 cgcatcaagg ttggcatagg cgcaaagcca aatcccgact gggaccttgc cgactgggtg
     5521 ctttcaaagt tcactcccga tgaaatgaaa aagcttgatg aaatttttga caatgccgaa
     5581 aaagcaatcg agctgattat tgacggcaaa accgaccgtg ctatgaattt gttcaactga
     5641 aaagggacaa agaaatgaat aatctcaact ttcttgttga tgtcctcaaa gagtcacccg
     5701 ttttcaaaac gcttgaaaag aatgtcaagc cgggcagaac ggtctgtgct tcgggacttt
     5761 caacaatcaa caaatcaaat atcatctatg cgctgtgccg cctcaagggc gtcacggcat
     5821 tttgtcttgc ctccgatgaa aaagaggcgc aaactttgtg caacgacctt tcgtgcatgg
     5881 gacttcgtgc ctatgtttat cctgtgcgtg acttcaactt tcttgacttt cagagccgtt
     5941 cgcacgagta cgagcacgca agacttaaag ttttgctcaa gcttatcaat catgaatgcg
     6001 atgttgcaat tgcgtgtgtt gatgcggcgg cacagcttac cgttccgaga aatgtgcttg
     6061 aacagtcggt tattgaattt gaagagggca gggagctctc gcttgaaaag gcgacaaagg
     6121 cgcttactct gctcggctac gaacgctttg atgcggtaga gggcagcggt cagttttcgc
     6181 tgagaggcgg tattctcgac ttctttatgc ccgacagcga ctatcccgtg agatgtgaat
     6241 tttggggcga tgaaattacc gacctcagct attttgacat tgaaacacag agaagattta
     6301 aaaaggctga caaaattacg ctttcgccct caaccgaaat cgtaattgag gacagagccg
     6361 cccttgcaga taaaattgaa cacaaggcaa agctcctccg ttccaagaac agcgccaaag
     6421 ccaaggaaaa acttttttca gaggcagagc ttatccgcag cggtgcaatg attgccaatg
     6481 cggacaaatt tatcaatcag atttacgata aacccgagtc gctttttgat tatctcgaca
     6541 gaaacaccct cgtttttgcg tccgaattta ctgcaattca ggaacgggga aagtcaatgg
     6601 actttatcag caatgaaacc cttatgcaag gctttgagga cggaactctt tgcagaggtt
     6661 ttgaccgctt tgcgctcaca ttcaacgaat gtaccgagtt tttgcaaagt cacggaacaa
     6721 tcgtgcttga aaactttgtt cacggaagta tgcctatcaa gctgtcggaa atcatcagct
     6781 tttcaacaaa acagctctcg gcatggggcg gttcgtataa acagctaaaa gaagatgttg
     6841 acggactttt tacccccgaa agcaagggcg ttatctttgc aggaacagaa agagccgcca
     6901 aaaatctttg tgacaccttt aacgcagacg gcataaacgc tgtttacagc gagggtgcgg
     6961 ataagatttc aaagggcgaa ctgcttgtta tgcagggtgc attgagtgcg ggctttgagt
     7021 atccgtcaca aaagtttttt gcaataacct acagtcaggt ttcgtaccgt cccgaaaagt
     7081 ccaaaaagaa gaagaaaaag acgggacagg aaatttacag cctttccgag cttgcccccg
     7141 gtgattatgt tgtacacaat gttcacggta tcggtgtgtt cggcggtata agaaaaattg
     7201 atactcacgg agttataaag gactatatca agattgacta cgcaaagggc gatgtgcttt
     7261 atgtgcctgt cacacagctt gatatggttg caaaatatat aggtccgaaa gaggattcaa
     7321 gggttaagct aaaccgactc ggttcgggcg actggcaaaa ggcaaaggca agggttaaaa
     7381 cctctgtcaa ggacattgcc aaggagctta ttgagcttta ctcacagcgt atgaaagcca
     7441 agggctatgc cttttcggca gataacgagt ggcagagaga ttttgaactc agctttgaat
     7501 atgacgaaac tcccgaccag ctgcgttgtt gtgaggaaat caagcatgat atgatgcgtt
     7561 cgtcgccgat ggacagactt ctctgcggtg atgtcggatt cggcaaaaca gaggttgcac
     7621 tccgtgccgc ctttaagtgt attgcagatt caaagcaatg cgcattgctc tgccccacaa
     7681 caattctcgc atggcagcat tatcagacgg taatcaagcg ttttgagggc tatcccgttc
     7741 gtgtggaact gctttcaaga ttccgtacag caaaacagca gaaagaaatt ctccaaaagc
     7801 taaagcgagg agaaattgat atgattgtcg gaactcacag acttgtgcag aaggatgttg
     7861 aattccgtga cctcggactt gcaatcatag atgaggaaca gcgtttcggt gttgcccaaa
     7921 aggagaggtt caaggaactg tgcaaaaatg tcgatgtgct tacgctctcg gctactccta
     7981 ttccgagaac cctcaatatg gcaatgagcg gtctgcgtga tatgagcgtt atcgaagaag
     8041 ctccgacaaa ccgtcagcct gttcagacct atgtgcttga acacgatgat gcggttatca
     8101 acgaggcaat ccgccgtgaa ctccgccgtg gcggtcaggt gttctatctt cacaacaatg
     8161 ttgaaacaat ttcttcatgt gccgcaaaaa ttcaggaggc tgttcccgag gcaaaaatcg
     8221 caatcggtca cggcaaaatg aaagagggcg aactcagcga agtttggcga cagatgcttg
     8281 aacaggagat tgatgtgctt gtctgcacga caattatcga aacgggtgtc gatttgccga
     8341 atgccaacac ccttattata gaaaatgccg actgtatggg actttcgcag ttgcatcagc
     8401 tcagaggccg tgtgggcaga agttcacgaa gggcatatgc ctactttacc ttcagacgga
     8461 acaaggttct cacggagatt cagcagaaaa ggcttgccgc aatcagggaa tttacagaat
     8521 tcggcagcgg cttccgcatt gcaatgcgtg accttgaact gcgaggtgcg ggcaatatta
     8581 tgggcgcaca gcagcacggg catatggaat cggtcggtta cgatatgtac ctcaagcttc
     8641 ttgatgagnn nnnnnnnnca gtaagcgagg aaaagggcga gcagaatgtc acaaaagacc
     8701 tcgactgtct tattgatatt tcggttgacg ctcatattcc cgaaagctat gttgaaagtc
     8761 ttacacttcg tcttgatgtt taccgcagaa ttgccgatat ccgcagtaac gaggacgcag
     8821 acgatgttat tgacgagctg caggacagat tcggcaagat tccgaactcg gttctcggac
     8881 ttatcgacat tgcccttgtg cgtaacaagg cttactcaat gggcatttac gaaatcagac
     8941 agaacggcac aacgctcatg ctctttgtaa acgaactcaa atcccctcag gttgccgact
     9001 tgctcattgc tttgggcgga aaggcaaagc ttgttgccgg agcaaaaccg tgcgttgcgg
     9061 ttgagtgcaa aaacagttcc gctccgcttg aaatcctcac caaaattttc ggtgtgcagt
     9121 aatttaacgg tatgaaaccg taatttgtta agaaaaaacc tcccgaaagt acaaaaatgc
     9181 tgtttttcgg tggaaattct caacaaaatt ttataaaaat gtagacaatg acgatttttt
     9241 tgtgtataat cgtaaggtac attatcctgt gaaagtgtgt ttgcaggaca ctttgcattt
     9301 aatttttcag aaggacggta aaaaactatg aagcctaata tcaaggttgg ttcattttta
     9361 cttgcaattg ttatgatgtt ctcagttttt gcaattgcag gttgtacacc cacaactctt
     9421 aacaaagaat ggtcgtataa gacaagcgac aacgagcttg ccatcggcgt ttatatctac
     9481 tcactcaatg cagcttacag tcaggctgag tcatttgcta aaaagcttga cgattatgat
     9541 tcgacatctg acaagtggct tgatgaaaaa atcaaggacg atgacggcaa cgagcaggtt
     9601 gcaagagagt ggattaaaga tcaggctaag aaaatgtgcc tcagctacct cgtagttgac
     9661 gaacagctta aaaaggaaaa tgtaaacatc ggtcaggcaa ctcttgactc cgcaacaagt
     9721 caggctgaat catactggaa tgtcggtccg tatgcgtcac agggttatgt aatgccgatg
     9781 aagaagcagt atgaaaagta cggcgtttca ctcgacagct ttgcatactg cacaactctc
     9841 tacaacacaa agtatgaggc tctcttcaaa gcagtttacg gcaagggcgg ttcaaaggaa
     9901 gtttccgacg ccgaccttac aaagtatttc aaggaaaact atacggacta ctcatatctt
     9961 cccgtgaacc tttatacatc aacaaaagat gaggcaggtt catcaaagaa tgttgcaatg
    10021 agcgacaagg aaatcaagaa ggttgaggat cagcttaacg gctacaagaa tgaccttaac
    10081 aagggcggtt cttttgatga tgttattgca tcatacaaga agtcaagcgg ttcgggaaca
    10141 gattcttctg tttcaaatgt agaagttctc gacaagagct caatcggtga cgagcttaaa
    10201 aaggctatcg gcaagcttga aacaggcaag gctgaaactc tcaaggtcgg cagcggcgac
    10261 agcgcaatct actatctcgt ttataagaaa gacgtcagca aggatgttga cagctatatc
    10321 ggcaacgaat caaacagagc cggtgtactt gcgtcaatga agtcagacga attttcaaag
    10381 tacattgaca gtcttgcaga gaaactcaag tatgaagaaa acacttcggt tattgacaag
    10441 tataagcccg aactcttctt cgttgcggtt gagcctacaa catcggcgtc aacaacatcg
    10501 gcatccgata aaagctcaaa ataattgata tcggacatac aacagacgca ttttcagatt
    10561 attctgaaga aaggggcttt tatgaaaaaa cattcaaagc tttgcgcaat ttttctgacg
    10621 gcacttttga gcatttctgc tgtgagcttt tccgtatcgg cagtcggtga ggaggttgat
    10681 cccggcggag attcaagcgt tattgttgat cccgtagagc cggagcccga gcccgagccc
    10741 gaaccggaac catcaccgga gcctgagccg cagccgcagc ccgatcctgt gtatccggat
    10801 tcttcggaca gttcatcata tgtagatccc ggctattcgt caagtgattc gtcatatgac
    10861 aacaactata actatgacag tagctatgac tacaacaata acagttatga caataattac
    10921 agcagcagtc agacttacta ctactctgag ccgtcatatg taggcggcgg tcagtcctat
    10981 gttgctccgt caaatactgc tccgtcagcg tcgctcatca agtctgacga taacattgac
    11041 gaaaaaactc tttcaagcaa cgactggtcg gatatcgcaa aaaatcttaa aaataccgac
    11101 acatcgagtt ctgacggtga tgactttaac ttcatcaaga acaataacag caagggtgac
    11161 aacggtcatt ggatgttcta tgtttcactc gttttactct cactcggtgt tgcaggcatt
    11221 gcgtactttg taatttcaac tgttctcaac agaaagaaga gagtcacagt cggcggcaga
    11281 agtaacgctc caaagagcaa atctgcacag ccacagcgtt acagcagtaa cgatgattat
    11341 gccgatgatt actcatcttc aggcagaaag tccgaaagac gcagtaagta cgatactgcc
    11401 gaaattcagc ttccaaagaa cagccaaaag ggcggaagaa gatacaagta attaaataac
    11461 tttataaaac aaaagcagtt ccaaaatcgg aactgctttt ttgtttgact gtgtaattaa
    11521 attttcgcat gacttctgtt tgaatgacaa aatcagtttt tgtcatacgg ctttttgtat
    11581 gcctctttga acggttttac agtgccgtcc ttttcttcaa cgcttacatt gtcaagtgtt
    11641 gcgttgaact gtgtgcggat ttcgccgaga taaattttgt acagctcttt ctgttctttt
    11701 tgctcagcct cggtaagtcc ctgttccttt ttcttttttg caagctcgtt aattctgtca
    11761 agcatctttt tatccataag tcaatcctcc cgaccgttat tttgtttttg agagcaatct
    11821 gctccaaagc tatgatatca cacaatcagc caaagtgcaa attttcgatt tctcggttga
    11881 aaacaaaaat tttttgtgct atcattcaat taaagccgtt atacggcaaa ttttttcggc
    11941 ggtgaattat ggaaaatttc agcttattac acccgacaaa ttcaaatgca tcctacagaa
    12001 ctttcaacaa agaggcactg aatgatttgt cggttgatta tatatgtgac gctctcacaa
    12061 aagagcagta tgaacgcaac agcataaagc agctgctgat aaatattacg gatgacgagg
    12121 aagttataaa atatcgttgc gatgtttttg aggattttct tcgttttccg aaacttcgtg
    12181 atgatctatc cgcactcctt gtaaagctga acgatttgcg tgaaattgag cgctttcaaa
    12241 aggataccga ggcatcttcc ttgtggcagc ttgtaaaccg acttcgtgaa atggacggct
    12301 atgtcgattg cattacaaga ataaaaaaca cgcttgaaac aattgaagta aagtcgcagg
    12361 gtctgcttga gctcaagaaa aatgttcagt caatttacaa tgacagcggt tttcccgaac
    12421 taaaaaagga tataatagat acgcttgcaa aggctcaaaa gctcaagagc attacactcg
    12481 gtgtgaatct tgacgatttg ctccgcccaa agagtgtggg tgtaatctca ctcaatgaca
    12541 aagaatttac cgacagcggt attctcaaaa gatttatgaa gtttacaaac cgtgacagcg
    12601 aactgcacca cggcaacgat gtttcgggat ttttaagctt tcatccgtcc aatccgtcaa
    12661 cctcgcagtt cgggctggga cagtttgttg tgggcgctca gcaggatgta aaccacacta
    12721 tgaacagctc actgaccggc gcagatccta tgtctgacgc actcaaaaat gttgtgaccg
    12781 atatcctccg cagaaccgta aatgacataa aatcaatgct caacagatat gtcaatgtca
    12841 acggctactc gttcgtttcg cttatgcccg aaatcatttt ctatattcgt tttgcagagc
    12901 tttgtgataa gatgaaaaag aaaaatcttc cgctgtgcaa ggcagaagtt ttgccgaaag
    12961 aggacagaaa ctgctgt
//
LOCUS       DAOMVE010000037        12838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1109_length_12838_cov_3.910976, whole genome shotgun sequence.
ACCESSION   DAOMVE010000037 DAOMVE010000000
VERSION     DAOMVE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12838
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1109_length_12838_cov_3.910976"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLS64_03360"
     CDS             <1..614
                     /locus_tag="PLS64_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRL42904.1"
                     /translation="YAMKNELCNKDYSEYVDIVKHKGRNPNKRDREKFTKAEIDRLWK
                     ASDDKYYQIVLMLIYSGVRISEILNLKREDVNLKEQYFDIKLSKTDNGIRRVPIADKV
                     LPFFKNWYNDGESEYLLHTENGNKFDYRNYYDSYFTPLMVNLNMDHTPHCTRHTCISM
                     LAEAQVDQTTIKKIVGHSGAMTMTERVYTHLDVEVLINAINKI"
     gene            complement(812..1213)
                     /gene="rpsI"
                     /locus_tag="PLS64_03365"
     CDS             complement(812..1213)
                     /gene="rpsI"
                     /locus_tag="PLS64_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRL42905.1"
                     /translation="MYDKTPYFYGTGRRKSSVARVRLYQGTGKITINDRDIDDYFGLE
                     TLKLIVRQPLALTETDEKFDIVCRVSGGGVTGQAGAIRHGISRALLQYDTENLRGKLK
                     KAGFLTRDPRMKERKKYGLKAARRAPQFSKR"
     gene            complement(1233..1661)
                     /gene="rplM"
                     /locus_tag="PLS64_03370"
     CDS             complement(1233..1661)
                     /gene="rplM"
                     /locus_tag="PLS64_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007045755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRL42906.1"
                     /translation="MSTFLAKPNEVERNWYVIDAAGKSLGRVAAQAATLLRGKNKATF
                     TPNVDCGDHVIIINCKDAVLTGNKLTQKKWQRHTGYIGHLKETRYDTLMATKPTKAME
                     LAVKGMLPKNTLGRAALLRLRLFEGAEHNHQAQKPAMIED"
     gene            1978..2391
                     /locus_tag="PLS64_03375"
     CDS             1978..2391
                     /locus_tag="PLS64_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42907.1"
                     /translation="MKNTKSAVLACVTSQYNCDRIIRIAKEIADSSDCELRVMSVLKP
                     MSDYSSVSDEIEYLYLIAKESGADMTVMFSDDAPKVTAEFVGDNNVERIVTGMHDGKD
                     NSFLVKFNELSPTTPITMVSKDNMVYSMDLCRSYS"
     gene            complement(2666..3190)
                     /locus_tag="PLS64_03380"
     CDS             complement(2666..3190)
                     /locus_tag="PLS64_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL42908.1"
                     /translation="MGFEEVKKFKYKFKRISSINIELIEEFSCGLEELDKKLIQMKEN
                     DEGTTFVFLDETNGQIIGYCTYCASGLKKAYENDSITYPAAEIKYFAIDKTYQHKSYD
                     DDFKFSDLMLCEVLKKLIEISEEAISFDYILLYSVPEAVNFYKRNGFCEFTEFMKKDS
                     YNYIDGCIPMFFTL"
     gene            complement(3172..3333)
                     /locus_tag="PLS64_03385"
     CDS             complement(3172..3333)
                     /locus_tag="PLS64_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42909.1"
                     /translation="MAVLAVPMDKTFEVAVDKVENFDKKAKEHSGKHLLQDRLKKHNK
                     GDVEWDLKK"
     gene            complement(3579..3666)
                     /locus_tag="PLS64_03390"
     tRNA            complement(3579..3666)
                     /locus_tag="PLS64_03390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:taa)
     gene            complement(3676..3750)
                     /locus_tag="PLS64_03395"
     tRNA            complement(3676..3750)
                     /locus_tag="PLS64_03395"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3715..3717),aa:Glu,seq:ttc)
     gene            complement(3756..3832)
                     /locus_tag="PLS64_03400"
     tRNA            complement(3756..3832)
                     /locus_tag="PLS64_03400"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3796..3798),aa:Met,seq:cat)
     gene            complement(4016..4966)
                     /locus_tag="PLS64_03405"
     CDS             complement(4016..4966)
                     /locus_tag="PLS64_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRL42910.1"
                     /translation="MYRLGIDLGGTNIVAGVVDENYEIIAKASCKTNVPRPESEICDS
                     MADVALKAIEKAKLTIDDIESIGIGVPGAVNPKTGVIEYSANLFFHNWQVVKMMEERL
                     NTKICVENDANAAALGEYLAGSAKGAKNAIAITLGTGIGGGIIINGKIYSGSNYAGAE
                     LGHMVIVKDGKECACGRKGCWEAYASATGLINLTKQEILKENFDFSYMLKSCDGDINK
                     VTGKTAFDAVLAGDANAKTVIDEYIGYLACGLVNIINIFQPDILCIGGGISNQGENLL
                     APLRTIVEKERYTKHNDKQTVICKASLGNDAGIIGAAFLE"
     gene            complement(5022..6500)
                     /gene="guaB"
                     /locus_tag="PLS64_03410"
     CDS             complement(5022..6500)
                     /gene="guaB"
                     /locus_tag="PLS64_03410"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRL42911.1"
                     /translation="MLNTSYDEKFGKKGLTFDDVLLIPGESDVEPKNVDVSTHLTKTI
                     KLNTPLMTAAMDTVTETDMAIAMAREGGVGIIHKNMSIEKQADQVDRVKRSENGVIVN
                     PFFLSPENTVRDADELMGKYKISGVPICRDGKLVGIITNRDMRFMTGSDFAQPISAVM
                     THENLVTAPVGTTLKQAQQILREHRIEKLPIVGKDGSLKGLITIKDIEKSVQYPNSAR
                     DDKGRLICGAAIGATKDVLDRVAALVESQVDVVVLDSAHGHNSNVVQSVAKVKKAYPN
                     LPLIAGNIATAEAAKALIDAGADAIKVGIGPGSICTTRIVAGIGVPQITAIYDAACEA
                     SKYGIPVIADGGIKYSGDIVKALAAGGSVVMVGSLVAGCAESPGDNEIYQGRQFKVYR
                     GMGSLGAMGKGSADRYFQASNNKFVPEGVEGRVPYKGPLSDTIYQMLGGLRAGMGYTG
                     CATIKDLHEKARFVQISGAGLRESHPHDIQITKEAPNYSYHS"
     gene            6736..7551
                     /locus_tag="PLS64_03415"
     CDS             6736..7551
                     /locus_tag="PLS64_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42912.1"
                     /translation="MNITKQIFKKSVLVGTIALSIAVFAGCSDSANSSTADTTKPTEA
                     QTTVQATEGTAKSGTTNESFDLKSLHTCKENDEDDLVGTWQITSGEGSQYTSFYYMFN
                     GEGKSILISGTSGYFGKYSYDTDDDNNPTFTTKLVFGLNGTYTYKLSDDKKTAELTNT
                     DTKAVSTLKKTDDPDFIPTPDKDASIDDKLVGCWKSESGEYLCFDKSGIMYQNLFDYM
                     FAYSNYTASEGKIVSTYTTSDKKTTDKYTYSVDGDTLTLNNDTYSRISFSDLK"
     gene            complement(7667..8065)
                     /locus_tag="PLS64_03420"
     CDS             complement(7667..8065)
                     /locus_tag="PLS64_03420"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HRL42913.1"
                     /translation="MKKRLLKFSTAAIILVAAGVLYYLIVSFTGFAIPCVFRKITGLY
                     CPGCGVTGMLTHIVRLDFKGAFECNQVLFVISPFILYLLGKMLYGYIRYGRLTLKKFD
                     TVLTFVLIGILLVFGVVRNLPPFDFLRPYG"
     gene            complement(8069..8422)
                     /locus_tag="PLS64_03425"
     CDS             complement(8069..8422)
                     /locus_tag="PLS64_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HRL42914.1"
                     /translation="MKQRSVGIAILLSIITCGIYGIYWLIMLNDETNYVSGHQQDGTS
                     GGVVFLLTLVTCGIYGYYWCYKQGEKLNEAKMQRGIMVDSSASVLYLILSIFGLSIVS
                     YALMQSELNKMLPPQ"
     gene            complement(8559..9839)
                     /gene="serS"
                     /locus_tag="PLS64_03430"
     CDS             complement(8559..9839)
                     /gene="serS"
                     /locus_tag="PLS64_03430"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRL42915.1"
                     /translation="MIDIKFLRENPDAVKENIKKKFQDYKLPLVDQVIELDEQSRAVK
                     KQADELRSNRNKISKQIGSLMAQGKKEEGMALKEEVTKQAQQLAELEKQESDLSAKVT
                     EIMMQIPNIIDPSVPIGKDDSQNVEIERFGDPFVPDFEIPYHTEIMEKFNGIDLDSAR
                     KVAGNGFYYLMGDIARLHSAVIAYARDFMINRGFTYCVPPFMIRSNVVTGVMSFAEMD
                     SMMYKIEGEDLYLIGTSEHSMIGKFIDTINKEENLPYTLTSYSPCFRKEKGAHGIEER
                     GVYRIHQFEKQEMIVVCKPEDSKMWFDKLWQNTVDLFRSMDIPVRTLECCSGDLADLK
                     VRSLDVEAWSPRQKKYFEVGSCSNLGDAQARRLKIRVTGEDGKKYFAHTLNNTVVAPP
                     RMLIAFLENNLNEDGTIRIPEALRPYMGGMEVIK"
     gene            complement(9864..10712)
                     /locus_tag="PLS64_03435"
     CDS             complement(9864..10712)
                     /locus_tag="PLS64_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRL42916.1"
                     /translation="MAKKLGGLGKGLNAIFIENDSETEGGTVTLKISEIEPNRTQPRK
                     EFDEQALSELAESISQHGLLQPLLVRPLTLGGYQIVAGERRYRASRMAGLTEVPVIIK
                     ELTDTETMEIALIENLQREDLSPVEEALGYKALIDEHGFSQEEVAKSVGKSRPAVANA
                     LRLLKLPDNIMEYLKDGKISAGHARALLTLENEELMTELADEIVAKDLSVRQVEKICK
                     KKPTVQKEEKPEKKPSFYSMVELALSESLGRKISVSKSKGKQGGVLQIEFYSDEELTE
                     LSNKLK"
     gene            complement(10714..11460)
                     /locus_tag="PLS64_03440"
     CDS             complement(10714..11460)
                     /locus_tag="PLS64_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL42917.1"
                     /translation="MIGVNGVGKTTTSVNLAACLGKMGKKTLLVDADPQGNATSGVAI
                     DKSKVKFSTYSILVDGAKAEQCVLTTPYDNLHILPSSLDLAAAELEIAEKPHREALLK
                     NALLPIKQYYDYIIIDCPPSLGLITANALTVADTFLVPIQCEYYALEGLSQLINTVRR
                     IKRQYNESIEIEGVLLTMYDGRLNLTQQVVDEVKKFFPRKVFKTVVPRGVRLSEAPSF
                     GMPVIYYDKSCKGSQAYMALAEEIVGKERG"
     assembly_gap    11479..11488
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11826..12683)
                     /locus_tag="PLS64_03445"
     CDS             complement(11826..12683)
                     /locus_tag="PLS64_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRL42918.1"
                     /translation="MLTNIEAMRLNFMLKNDNKVLEIPIIKIRPNKAQPRKTFDETEL
                     AALSASISENGILQPLSVRKISAAEYELVAGERRLRASVLAGLRKVPCIVLKCSEKES
                     AIYALLENLQRSDLGIFEEARGISKLIRRYGLTQEEAANRLGKSQSTIANKLRLLRLT
                     YEEQEWIVNAGLSERHARALLRIEDDALRHEALSKIISENLNTSQTDELVGILINSVP
                     KTTKSKGTSKAVIKDLRIFLNTINKAIDTMRLAGIDAQSDKKDTDNFIEYTIRIPKRH
                     QEPDAEKTA"
BASE COUNT         3692 a         2810 c         2406 g         3920 t
ORIGIN      
        1 aatacgctat gaaaaatgag ctgtgcaaca aggattattc cgaatatgtg gacattgtaa
       61 agcataaagg ccgcaacccg aacaaaaggg atcgggaaaa gttcaccaaa gcagaaatcg
      121 accgcttatg gaaagcgtca gatgacaagt attatcagat cgtcctcatg ctgatttatt
      181 cgggtgtgag gatttccgaa atactgaatc taaaacgaga agatgtaaac ctgaaagaac
      241 aatattttga tatcaagctc agcaaaactg acaacggaat cagacgagta ccgatcgccg
      301 acaaggtact gccgttcttt aagaactggt acaacgacgg cgagagcgaa tatctgcttc
      361 acaccgaaaa tggaaacaag tttgattacc gcaattacta cgatagttat tttacgccgc
      421 ttatggtcaa tctgaatatg gatcacacac cgcattgtac acgacacaca tgtatctcaa
      481 tgctggcgga agcacaggtt gaccaaacca caattaaaaa gattgtagga cactcgggcg
      541 ctatgacgat gaccgaaaga gtgtacacac atttagacgt tgaggtgcta atcaatgcga
      601 tcaataaaat ctaaaagcaa aaaaagaaag agatgtcttt ctgaacttag aaaaacatct
      661 cttttgattg cttattgagg ttgagaactg ttgctactta tgtgttactt gcttgttgct
      721 tatctaacac atttaagcac atctcaaacc gtctgaatac tccaaaagcc ctgtaaacag
      781 tagccttgaa gcgtgctgga aagcacaaag attatctctt tgagaactga ggagctctac
      841 gagctgcttt aagaccgtat ttcttacgct ctttcattct cggatcacga gttaagaagc
      901 ctgccttctt gagtttgcca cggagattct ctgtgtcata ctgaagaaga gctcttgaaa
      961 taccgtgacg gatagcaccg gcctggcctg ttacgccgcc gcctgatact ctgcaaacga
     1021 tgtcgaactt ctcgtctgtt tctgtaagag cgagaggctg tcttacgatg agcttgagtg
     1081 tttcaagacc aaagtaatcg tcaatgtcac gatcgttgat tgtgattttg cctgtgccct
     1141 gataaagtct tacacgagca acagaacttt ttcttctgcc tgtaccgtaa aaataaggtg
     1201 tcttatcgta cattatcttt tgcctccttc aattagtctt caatcattgc aggcttctga
     1261 gcctgatgat tgtgttctgc accctcaaag agtcttaatc ttaaaagagc tgctctgccg
     1321 agtgtatttt taggaagcat acccttaaca gcaagctcca tagcctttgt aggctttgtt
     1381 gccataagtg tatcgtaacg agtttcctta agatggccga tatagcctgt gtggcgctgc
     1441 cacttcttct gtgtgagctt gttacctgta agaacagcgt ccttgcagtt gatgatgatt
     1501 acatgatcac cacagtcaac attgggagta aaagtagcct tgttcttacc tctgaggaga
     1561 gtagctgcct gagctgctac acgaccgagt gacttgccgg ctgcatcaat tacataccag
     1621 ttgcgctcaa cttcgtttgg tttagctaaa aaagttgaca taacaatatc ctcctgtatt
     1681 ttttcactgt gagattgatt atattacaat ttatgcccat tgtcaacact tttttgaaaa
     1741 aatttaattt agctttgaac aatctctcaa atacgcttgt ttactctcat atactctcgt
     1801 atactcacat actattttta aaataatttt ctgaaaatgc cgataaatac tgaatttttt
     1861 aaattttgcg cacatttatt aaaaaagcaa aaattcacaa taagttcaca atatgaatat
     1921 ttttccgtca aaaacggctg ttatgatata gtcacaataa aaaaggaggt gaaaaaattg
     1981 aaaaacacga agtcggcagt tcttgcctgc gtcacaagtc agtacaactg tgacagaatt
     2041 atccgaattg ccaaagaaat tgccgacagc tctgattgcg aactcagagt tatgagcgtt
     2101 ttaaaaccga tgtcagacta ttcgtcagta agtgatgaaa tcgagtatct ttaccttatt
     2161 gcaaaggaat cgggtgcaga tatgaccgta atgttttcgg atgacgcacc aaaggttact
     2221 gccgaatttg taggtgacaa caatgttgaa cgcattgtaa cgggtatgca cgacggaaaa
     2281 gacaacagct ttcttgtaaa gtttaacgag ctttccccca caacccccat aaccatggtt
     2341 tcaaaggaca atatggttta cagcatggat ttgtgcagat cctattcata aatttttttg
     2401 atacaaattt cctttctctc caccaaacat acataaatac aattttatta acaccgaaga
     2461 atcgtataaa tctttcttct taaaatatac tctctccgaa aatgacctgc tgtttttcag
     2521 taggtcattt tcatatacat ctgccatcgg cgaactatat ttgctacgca agctatacag
     2581 aaactgtttc aacataagtt tatgttccca cccaaattat aaaagccttg caattttttt
     2641 acttgcaagg ctttgttgtg tttaattata atgtaaaaaa catcggaata cacccgtcaa
     2701 tatagttgta agaatccttt ttcataaact ctgtaaattc acaaaatcca tttcttttgt
     2761 aaaagtttac agcctcgggt actgaataca ataagatata atcaaaagaa attgcttctt
     2821 cggaaatctc aatcaatttt ttcaaaacct cacacaacat taaatcgctg aatttaaaat
     2881 catcgtcata agatttatgc tgataggttt tatctattgc aaaatatttt atttctgctg
     2941 caggataagt tattgaatca ttttcataag cttttttcag tcctgatgca caataggtac
     3001 aatatccgat tatctgacca tttgtttcat ccagaaaaac aaatgtagtt ccctcgtcat
     3061 tttctttcat ctgtataagc tttttgtcaa gttcttctaa accacaagaa aattcctcaa
     3121 ttaattcaat gttaattgag gaaattctct taaatttata tttaaacttt tttacttctt
     3181 caaatcccac tcaacatcac ctttgttgtg ctttttcaac ctatcctgaa gaagatgttt
     3241 tccggaatgc tcttttgctt ttttatcgaa attttcaacc ttatcaacag caacttcaaa
     3301 tgttttatcc attggtactg caagaacagc catattagtt tcctccttcg tattattaat
     3361 agcacaccac acccttttct ttagcataca aatatctgta tgcaacaaaa aagtatttat
     3421 actatctgcc ttaattttaa actaataaac atatcttgtc aatgatatat tcacaaataa
     3481 tcaagaaaga ccatccaaaa cggatggtcc ttaaacagca acaactatac aattaaaaaa
     3541 tcaccgaaac gaaaaaaaca gccctcgtgg ctgttttttg gtgccggtga ccggacttga
     3601 accggtacgg tcgcaatgac cgagggattt taagtccctt gtgtctgcct attccaccac
     3661 accggcaaat atatttggtg acccggacga gaatcgaact cgtgttaccg ccgtgaaagg
     3721 gcggtgtctt aaccgcttga ccaccgggcc aaatatggta gcggaaatag gacttgaacc
     3781 tacgacactt cgggtatgaa ccgaatgctc tagccagctg agctattccg ccatgtaata
     3841 catgaggatt tctaaccgaa atcagcaaga tttattatag ggcaaaaagg acgatttgtc
     3901 aacacttttt ttaatttttt tcaaaaaagt ttttattatt ttaaaaatcc gcttttcggg
     3961 cagaaaatac atttttccgc ccgaaattac gaagtaaatt ttaacagttt gtcaattatt
     4021 caagaaaagc cgcaccgata atgcctgcat cattgccaag gcttgccttg cagatgactg
     4081 tctgcttatc gttatgcttt gtatatcttt ccttttcaac aatcgtacgg agcggagcaa
     4141 gaagattctc gccctgattt gaaataccgc cgccgataca aaggatatcc ggctggaaaa
     4201 tattaataat attcacaaga ccgcaagcga gataaccgat atactcgtca attacggttt
     4261 tggcatttgc atcacctgca agaacagcat caaacgcagt cttgcctgta accttgttaa
     4321 tatcaccgtc acatgacttg agcatatagc tgaagtcgaa gttctccttg aggatttcct
     4381 gctttgtgag gttaataaga cctgttgcag acgcatacgc ctcccaacag ccctttctgc
     4441 cgcacgcaca ttccttaccg tctttaacga taaccatatg tccaagctca gcgcctgcat
     4501 agttagaacc gctgtaaatc ttgccgttaa taatgatacc gccgccgata cctgtgccga
     4561 gtgttattgc aatcgcattt tttgcaccct ttgcactgcc tgcaagatac tcgccgaggg
     4621 cagcagcgtt tgcatcgttt tcaacgcaaa tctttgtatt gagtctttcc tccatcatct
     4681 tgacaacctg ccagttatgg aagaaaagat tagccgaata ctctataacg cctgttttcg
     4741 gattaaccgc acccggtaca ccgataccga ttgactcaat atcatcaatt gtgagttttg
     4801 ctttttcaat tgcctttaat gcaacatctg ccatactgtc gcagatttcg ctttcgggac
     4861 ggggtacatt tgttttacag cttgcctttg cgataatttc gtaattttcg tcaacaacac
     4921 cggctacaat gtttgttccg cccagatcaa ttccgagtct gtacataaac tgtcggcaca
     4981 tccttttctt ttattttatg caaatattta tgatttatca attatgaatg ataagagtag
     5041 ttaggagctt cttttgtaat ctgaatatca tgcggatggc tttcacgcag acctgcacct
     5101 gaaatctgaa caaatcttgc cttctcgtga agatccttga ttgttgcaca gcctgtgtag
     5161 cccatacctg cacgaagtcc gccgagcatc tgataaattg tgtctgaaag aggtcccttg
     5221 taaggtacac gaccttcaac accttcggga acaaacttgt tgttacttgc ctggaagtat
     5281 ctgtctgcac tgcctttgcc cattgctccg agactgccca tgcctctgta aaccttaaac
     5341 tgtctgccct gatagatttc gttgtctccg gggctttcgg cacaacctgc aacaagtgaa
     5401 ccgaccatta caacactgcc gcctgcggca agtgccttaa cgatatcacc gctgtacttg
     5461 ataccgccgt ctgcgattac agggataccg tactttgacg cttcacaagc ggcgtcatag
     5521 atagctgtaa tctgaggaac accgatacct gcaacgattc ttgttgtaca gatagaaccc
     5581 ggaccgatac caaccttaat agcgtctgcg ccggcatcaa taagagcctt tgctgcttct
     5641 gccgtagcaa tgttacctgc gataagagga aggttcggat atgctttctt aacctttgca
     5701 acagactgaa caacatttga attgtgaccg tgtgcagagt caagaacaac aacatcaacc
     5761 tgtgactcaa caagagcggc aactctgtcg agaacatcct ttgtagcacc gatagcagca
     5821 ccgcagatga gtctgccctt gtcatcacgg gcagagttgg gatactgaac gctcttttca
     5881 atatctttaa ttgtgataag tcctttaaga ctgccgtcct tgccgacaat cgggagcttt
     5941 tcgattctgt gctcacggag aatctgctga gcctgcttaa gagttgtgcc gacaggagcg
     6001 gtaacaaggt tttcgtgcgt cataacagca gaaatcggct gtgcaaaatc ggagcctgtc
     6061 ataaaacgca tatcacggtt tgtaataatg ccgacaagct tgccgtcacg gcaaatcgga
     6121 acacctgaaa ttttgtactt acccataagt tcatctgcat ctcttactgt gttctcggga
     6181 gaaaggaaga acggattaac gataacaccg ttttcacttc tctttactct gtcaacctga
     6241 tctgcctgct tttcaattga catattctta tgaataatac caacgccgcc ctcacgagcc
     6301 atggcaattg ccatatctgt ttcggtaact gtatccatag cggcagtcat aagcggagtg
     6361 ttaagcttga ttgtcttggt aagatgagtt gacacatcaa cattcttggg ttcaacatct
     6421 gattcgcccg gaataaggag aacatcgtcg aatgtaaggc ccttcttgcc aaatttttcg
     6481 tcataacttg tattcagcat attattcctc catactacaa aattcgattt ttattgttac
     6541 ctattattat aacaaatcga cctgtgatta tcaagacaga aaaagttttt ttaaacaaaa
     6601 aatttgtaat tcatttttct gtattgacaa tatgttccgt aaattataaa atagtattgt
     6661 attgcctgtt ttactgaata tttgctttat tttggttacc gtgacaggct tattatgtga
     6721 taacagaggt ataaaatgaa cataacaaaa cagattttca aaaaaagcgt tttagtcggt
     6781 acaatcgctc tttcgattgc agtatttgca gggtgttccg actcggcaaa ttcttcaaca
     6841 gctgatacca caaaacccac tgaagctcag acaaccgtac aggctactga aggcactgca
     6901 aaatccggta cgacgaatga atcgtttgac ttgaaatcat tgcatacctg caaagaaaat
     6961 gacgaagatg accttgtagg cacatggcag ataacctcgg gcgagggctc gcagtataca
     7021 agcttttact atatgtttaa cggcgaaggc aagtctatac tcatcagcgg aacttcggga
     7081 tatttcggca aatacagcta cgataccgat gatgacaaca atccgacatt taccacaaag
     7141 cttgttttcg gactgaacgg aacttacacc tacaagcttt cagatgacaa aaaaactgcc
     7201 gagctgacaa atacggatac aaaggcggtt tcaacgctca agaaaacaga cgatcccgac
     7261 tttattccca cccccgacaa agacgcatca attgatgata agcttgtagg ctgttggaaa
     7321 tcagaaagcg gtgagtatct ctgctttgac aagagcggaa ttatgtatca gaatcttttt
     7381 gactatatgt ttgcatattc aaactacacg gcaagcgaag gtaaaattgt ttcaacatac
     7441 acaacctccg acaaaaaaac aacagataaa tacacatact ctgttgacgg cgacactctt
     7501 acactcaaca atgatacata ctcaagaatt tctttcagcg atttaaagtg atgaatcacc
     7561 gttgattcaa tgttgaaaac ttttcgtatt ttcttaacat cataacaaca atccccgaac
     7621 acaaaaatgt tcggggattt ctaattttca gaaagttatt aacatcttat ccatacggtc
     7681 ttaaaaagtc aaaaggcgga agatttctga caactccgaa aacgagcagt attcctatca
     7741 gcacaaatgt cagaaccgtg tcgaattttt tcagagtaag tctgccgtac cttatgtagc
     7801 cgtaaagcat ttttccgagc agatacagaa tgaacggtga gattacaaac aaaacctgat
     7861 tgcactcaaa agcaccctta aaatcaagcc ttacaatatg agtcaacatt cccgtaacac
     7921 cgcagccggg gcaataaagt cccgttatct ttcggaatac acacggaatt gcaaagccgg
     7981 taaacgaaac aattaaataa tataaaacac cggctgcgac caaaataatc gcagccgttg
     8041 aaaattttaa taatcttttt ttcattgatt actgtggagg aagcatcttg ttaagctcac
     8101 tctgcataag agcataagaa acaatgctaa gaccaaagat tgaaagtata agataaagta
     8161 ccgaagcact tgaatcaacc ataatacctc tctgcatctt tgcttcattg agcttttcgc
     8221 cctgcttgta gcaccagtaa taaccgtaaa taccgcaagt cacaagtgtg agcagaaata
     8281 caactccgcc tgatgtaccg tcctgctgat gaccggaaac atagtttgtt tcgtcattga
     8341 gcataatgag ccagtaaatt ccgtaaatac cgcaagtgat gattgaaaga agaattgcaa
     8401 tacctacact tctctgtttc ataataatta cctccgttaa cctctttcaa gggattttag
     8461 caaggtaaat tataccatta gtacggtaca atgtcaatat aattttgttt tgatatttgc
     8521 tatagtttgc tgatttttgt gagaacacag tatgttgatt acttgataac ttccataccg
     8581 cccatgtaag gacgaagagc ctcaggaatc ctgattgtgc cgtcttcgtt aaggttgttc
     8641 tcaaggaacg caatgagcat tctcggaggt gcaacaactg tgttgttaag agtatgtgca
     8701 aagtatttct tgccgtcctc gcctgtaact ctgattttaa ggcggcgagc ctgtgcgtca
     8761 ccgagatttg aacatgaacc aacctcaaaa tatttcttct gacgaggtga ccaggcttca
     8821 acatcaagtg aacgaacctt gaggtcagca aggtcgcccg aacaacattc gagtgtacga
     8881 acaggaatat ccattgaacg gaaaaggtct actgtgttct gccaaagctt gtcaaaccac
     8941 attttgctgt cctcaggctt acaaacaaca atcatttcct gcttttcaaa ctgatgaatt
     9001 ctgtaaacgc ctctctcctc aatgccgtga gcaccctttt cttttctgaa gcaaggcgaa
     9061 tagcttgtga gagtgtaagg aagattttct tccttgttga ttgtgtcaat gaacttacca
     9121 atcattgaat gttcgcttgt gccgataaga taaaggtcct cgccctcaat cttgtacatc
     9181 attgaatcca tctcggcaaa gctcataacg cctgttacaa cattacttct gatcataaaa
     9241 ggcggtacac agtatgtgaa acctctgtta atcataaaat cacgggcata tgcgattact
     9301 gcactgtgaa gtcttgcaat atcgcccata agataataga aaccgttacc cgcaaccttt
     9361 cttgcagaat caaggtcgat accattgaat ttttccataa tttctgtgtg ataaggaatt
     9421 tcaaaatcag gtacaaacgg atcgccgaac ctctcaattt caacattctg actgtcatcc
     9481 ttaccaatcg gaacagacgg atcaataatg tttggaatct gcatcatgat ttccgttacc
     9541 tttgcagaaa ggtctgattc ctgcttttca agctctgcaa gctgttgtgc ctgctttgta
     9601 acttcttctt taagagccat accctcttct tttttgccct gagccataag agagccaatc
     9661 tgctttgaaa ttttgtttct gtttgaacgg agttcatcag cctgtttttt aacggctctg
     9721 ctctgctcat caagctcaat tacctgatca acaagcggaa gcttgtaatc ctgaaacttc
     9781 tttttgatat tttcttttac tgcgtcggga ttttctctta aaaatttaat atctatcatt
     9841 ttaaaatacc tctttatgta atgttattta agtttgtttg aaagctctgt aagttcttcg
     9901 tccgaataga attcaatctg aagaacaccg ccctgcttgc ccttgctctt tgaaacgctg
     9961 atttttcgtc caaggctttc cgagagtgca agttcaacca tgctgtagaa cgaaggcttt
    10021 ttctcaggct tctcttcctt ctgaactgtc ggttttttct tgcagatttt ctcaacctgt
    10081 cttaccgaaa gatcttttgc aacaatttca tctgcaagct ccgtcataag ttcttcattc
    10141 tcaagcgtaa gcaatgctct tgcatgacct gccgagattt tgccgtcttt gagatattcc
    10201 attatattat cgggaagttt aagaagtctt aatgcatttg caactgcggg acgggatttt
    10261 ccgaccgact ttgcaacttc ttcctgtgaa aaaccatgtt catcaataag agccttgtag
    10321 ccgagtgcct cttcaacagg gcttaaatcc tcacgctgaa gattttcaat aagagcaatc
    10381 tccattgttt cggtatcggt aagctccttg attataacag ggacttcggt aaggcctgcc
    10441 atacggcttg ctctgtatct tctctcacct gcaacaatct gataaccgcc gagtgtaagc
    10501 ggacgaacaa gcaacggctg taaaagtccg tgctgtgaaa tactctctgc aagctccgac
    10561 aaagcctgtt cgtcaaattc ttttctcggc tgagtgcggt tcggttcaat ctccgaaatt
    10621 ttaagtgtta ctgttccgcc ctctgtttcg gaatcgttct ctataaatat tgcgttaagg
    10681 ccttttccaa gaccaccgag tttttttgcc aaatcagcct ctctcctttc cgactatttc
    10741 ctcggcaagt gccatatatg cctgactgcc cttacaggat ttgtcgtagt agataacagg
    10801 cataccaaag ctcggtgctt cggaaagtct tactcccctc ggaacaactg ttttgaaaac
    10861 ctttcgtggg aagaattttt tcacttcatc tactacctgt tgtgtaagat taagtcgtcc
    10921 gtcatacatt gttagcagaa cgccttcaat ttcaattgat tcgttgtact gtctttttat
    10981 tcttcttacc gtgttaataa gctgtgacaa tccctcaagt gcataatatt cgcattgaat
    11041 cggcacaaga aatgtgtcgg caaccgtcaa cgcatttgcc gtgataagtc cgagtgacgg
    11101 aggacagtcg atgattatgt aatcataata ctgctttatc gggagcaaag cattctttaa
    11161 aagtgcctct ctgtgcggtt tttctgcaat ttcaagctct gcggcagcca aatcaaggct
    11221 tgacggaaga atatgcagat tgtcatacgg ggttgtaaga acacattgtt cggcttttgc
    11281 accgtcaaca agaattgagt aagttgaaaa cttaactttg ctcttatcaa tggcgacacc
    11341 gcttgtggca tttccctgtg gatcagcgtc aacaagcaat gttttctttc ccatttttcc
    11401 cagacaagcc gcaagattaa cggaggttgt ggtctttccc acaccattga ctccgatcac
    11461 aaggacaacg gatgtctcnn nnnnnnnngg tctttcccac acctcccttt tggtttgata
    11521 ttgcaattat ttttgccaaa aaatcacctt gtcttttgtt tttatgattt ttatatcttt
    11581 tactttaaaa ttatatcata atgcacacca tttttaaagc tattttgaag attttttggg
    11641 atttaatgca actttttttc ttaatcaaaa gcaaatgttt cacgtgaaac atttcctgtg
    11701 taaccaaaaa ataaaaacta tacaacgcaa aaagtcgcac attactgtgc gactctttgc
    11761 aaggggttta gataaggaaa tgggtatgag tggaacggat gctttcgcac cctctgaaaa
    11821 gtgaattaag cagttttttc tgcatcaggc tcttgatgtc ttttaggaat ccgaattgtg
    11881 tattcaataa aattgtctgt gtcttttttg tctgattgtg cgtcgattcc tgcaagacgc
    11941 attgtatcaa tagccttgtt gattgtgtta agaaaaattc gtaagtcttt tatcaccgct
    12001 ttgcttgtac ctttggattt tgttgttttt ggtactgaat taattaaaat gccgacgagt
    12061 tcatcagtct gacttgtgtt gagattctcc gaaattattt ttgacagagc ctcatgtctt
    12121 aaagcgtcat cttcaattct taaaagagcc cttgcgtgtc tttcgctcaa tcctgcattt
    12181 acaatccatt cctgctcctc gtatgtaagg cgaagaagtc ttagcttgtt tgcaattgtt
    12241 gattggcttt tgcccagtcg gttcgctgct tcctcttggg taagaccgta gcgcctgatc
    12301 agctttgaaa tcccgcgagc ttcctcgaat ataccgaggt cgcttcgctg gagattttca
    12361 aggagagcat aaatggctga ctctttttcc gaacacttta aaacgatgca cggcaccttt
    12421 cgtaatcctg ccaaaaccga tgccctgagg cggcgctcac ctgccacaag ttcatactcc
    12481 gcagccgaga tttttctcac cgacaacggt tgcaagatac cgttttcgct gattgatgcg
    12541 gagagagcgg caagttcggt ttcgtcaaag gttttgcgag gttgtgcttt gttcggtctg
    12601 attttaataa tcggaatctc taaaactttg ttatcgtttt tgagcataaa attcaaccgc
    12661 atcgcctcaa tgttcgttag catcaggggt tgtccctgtg ctattataat aacatagaca
    12721 cggtgcggtt gttgtcgctt tgtgagcaac ttttttaaga agttttacca caaaatggta
    12781 atttatcaaa aaagctgtcg atatttatca caacggttgt gactttattt tgcccgaa
//
LOCUS       DAOMVE010000038        12813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1113_length_12813_cov_3.340963, whole genome shotgun sequence.
ACCESSION   DAOMVE010000038 DAOMVE010000000
VERSION     DAOMVE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12813
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1113_length_12813_cov_3.340963"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..174)
                     /locus_tag="PLS64_03450"
     tRNA            complement(100..174)
                     /locus_tag="PLS64_03450"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(140..142),aa:Cys,seq:gca)
     gene            complement(432..977)
                     /locus_tag="PLS64_03455"
     CDS             complement(432..977)
                     /locus_tag="PLS64_03455"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRL42919.1"
                     /translation="MKVFNCIFGILSIIGAIYCMFYPGLTFLNTGWIVTILLGVWGIC
                     SVIDYFAKRKKAKAEQSEAIMGTLGLVVGIAAAVISILAMFMPGIRLMFDVIILCIFS
                     GWLVVDGISSVAMSLKVKKASSSGAWLIPLFCGILVIIGGIYGFFHLIFAAQTIGFMI
                     GVLLSIYGVKLILSAFEKPSV"
     gene            complement(996..>2098)
                     /locus_tag="PLS64_03460"
     CDS             complement(996..>2098)
                     /locus_tag="PLS64_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HRL42920.1"
                     /translation="PAEMNTYLPKYKALWMAENCSGTMHNLYTLRGAEVRDGNAWAQY
                     IMEAKELFGDKTEVVFQAHNWPHWGNDVINDYMANTASVYKYIFSQTLMYINQGYTST
                     EIANMMELPDELNKVWYTRQYYGTLKHNVKAVYQKYMGWYDENPIHLDELEPTEYSKK
                     LVEYLGDTDKVLEMAKKDFDKGEYQWVAQITNTLVYADPENKDARYLCADALEQLGYQ
                     AESGAWRNAYLTGAYELRNGTKNYPNSEGSGATALGMSTETMLDYLGICLDEKKLEDQ
                     NLVINLEVTDKNAKYLLRINHGVLIYSQEKWSDKADATIKTKSAGILGIAQNNQKLMD
                     AGIEKVEGNSDIIKTLTSSVAEFPLYFNIIEP"
     assembly_gap    2099..2108
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2109..>2992)
                     /locus_tag="PLS64_03465"
     CDS             complement(<2109..>2992)
                     /locus_tag="PLS64_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL42921.1"
                     /translation="LALAATLIASSALCGCQQSDSSTSAKFNSDVKDASKYTADENAQ
                     VYKTLDFSDEQEKEFAKKGFITAPDKLEIKTENGTVAWSQTAYDFIRNSSTPDSANPS
                     LWRNTELNSLYGLFEVVDGVYQVRGYDVANVTFVKSDNGWIVFDCTTSSETAKAALEL
                     LESKFGKAHIAAVVVSHAHIDHYGGIGGLIDEKNVADSSLPLDEQIKSGKTLIIVPEG
                     YEKAVMEENVFAGNAMKRRTNFQYGSLLDKGGKGSLSVGIGLTPSSGTTTYISPSYEV
                     KKATETIKVDGVEMVFQL"
     assembly_gap    2993..3002
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3196..3918
                     /locus_tag="PLS64_03470"
     CDS             3196..3918
                     /locus_tag="PLS64_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRL42922.1"
                     /translation="MRIAIVDDCENERNELCKRLSQSSFSRSYDIEICEYNSGTDFLN
                     EAMQNRFDLVFMDIYMEKENGIDAAQKLRKFDKDCLLVFTTTSTDHALEGFRVRALHY
                     LVKPYSDEELDMLLDEISQKISVEEKYIEIPSASDSLRIKLCDILYAEHFKHCIHIHC
                     TDKSEKSVRSTFAEFVMLFDDGDNFFVCNRGIIVNLEHIRDMNGNEFVLDNGERISIS
                     RSLVKSAKSTFGEYIFGRRDLH"
     gene            3915..5228
                     /locus_tag="PLS64_03475"
     CDS             3915..5228
                     /locus_tag="PLS64_03475"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRL42923.1"
                     /translation="MTNVFCPMIELGAIIPGVVLAYLPIKKHLKLSKLRLFPVMILSL
                     VCLCTLCGFVCYRYNLKSFVLAIPIVLALSAAYCCSLKTSMWKSVSVLLAVCGVFACI
                     ASITRAVDSITFPQNTTPWFEIQSAVLFNILCWMFVILAWYPATHGVSELLDDENIAQ
                     TWYVFWILPIIFILLNFFIIPWNPNILHTGRIMQGYIVISVSLLVLLLIFYALLYFIA
                     KSLNRNNKLAQKNQFLNMQRAQYDALKVAIEETRQARHDMRHHFAVLNALAEQKNWEE
                     LEKYLSSASQNIPDTELVLCKNHAVNAIIGHYYSKYKSNGIPFKLKIDIPEKLTVSES
                     DICLVIANLLENAFEASMKLDRDKRQIKMYARPHSEKILLISVENAFNGEITEKDGFF
                     GSSKRNGNGIGTQSVRRIAEKDGGYCRFSHENGIFTADVMLHGKN"
     gene            complement(5279..6301)
                     /locus_tag="PLS64_03480"
     CDS             complement(5279..6301)
                     /locus_tag="PLS64_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HRL42924.1"
                     /translation="MIDARKHTLDSVKKLTDYSDFNLYKMDICYDYSIDKIIDRGIFD
                     DESALGAILQEVLPDYPVNLKMPNFGCSAFTMKNPDSVMMGRNYDFKNDTSSMLVYCS
                     PKDGYRSVAFAALDNVGANNPELSDETRLATLLSPFISLDGINEKGVSIAVLTLDSKP
                     VRQQSGKPVIATTLAIRLILDRAASTEEAVTLFEKYDMFASSGRDYHFYVTDSNGDGR
                     VIEYDCNSDDRKLVVTKSPAVTNFFVMYKNFVKPYQKNGVYGHGRERYDKIINILEKN
                     NSYTKETAWEALVSASQAPNPNDITSNTQWSIVYDNTNLTADISIRRDWNTVTKYSLK
                     ENKITE"
     gene            complement(6471..7532)
                     /gene="trpS"
                     /locus_tag="PLS64_03485"
     CDS             complement(6471..7532)
                     /gene="trpS"
                     /locus_tag="PLS64_03485"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRL42925.1"
                     /translation="MKKVILTGDRPTGRLHVGHYAGSLKERVKLQNSGEYDDIYIMIA
                     DAQALTDNAEHPEKVRQNILQVALDYLACGIDPNKSTIFVQSMIPELTELTFYYMNLV
                     TVSRVQRNPTVKAEIQMRNFEASIPVGFFCYPISQAADITAFRATAVPVGEDQLPMLE
                     QCKEIVHKFNSVYGETLTEPQIILPSNKACLRLPGIDGKAKMSKSLGNCIYLAEEPED
                     IKKKVMSMFTDPNHLRVEDPGKVEGNPVFIYLDAFCRDEYFAEFWNDYSCLDELKAHY
                     QRGGLGDVKVKKFLNNVMQEELRPIRERRKEWENHLPDVLDILKEGSKVAKETAAKTL
                     EDVRHSMRIDYFTDNNLLK"
     gene            7774..8460
                     /locus_tag="PLS64_03490"
     CDS             7774..8460
                     /locus_tag="PLS64_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRL42926.1"
                     /translation="MEYNYHKNAGQLAFIKFVKGLVHLLIRPKVEWKDKSLKKSLKGK
                     PVVFVCNHTHHFDGVVISSVLSRYKPYMLVKKSWYDKSGTGSFIRICRSIPVDFDTMD
                     TNWYAQSEGAVENGYSMLIFPEGGIAREGKMLPFKPGAALLAAKKGLDIIPIASLGEY
                     KILFGKRQKILIGNPIKSECPENVRYSKYAKEISAEAEKQVKKLYLELEEKYGKGKVY
                     YKEDFSSENL"
     gene            8501..8743
                     /locus_tag="PLS64_03495"
     CDS             8501..8743
                     /locus_tag="PLS64_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HRL42927.1"
                     /translation="MNNAIADQIKEMLVENLRIPENILTYDSELFGDEIGLDSIDSIE
                     IVAGIDTLFGIDMTGADREHFQSIRALTEYVESKQG"
     gene            8745..10943
                     /locus_tag="PLS64_03500"
     CDS             8745..10943
                     /locus_tag="PLS64_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HRL42928.1"
                     /translation="MADKKRCVVTGLGLICGVGDNVKDCWDAVTLGHSGIDEVKSVDT
                     SNCYAHKGAEVDKASSELSPENYDRSSLLCIHAANEAFEDANIDASEKNIGVILGSCV
                     GGAASIDKYYTDEIKGDGGKKEDIFKMGASAIANNVSAHFGLEGITANIVNACAAGTM
                     SIGYACDLIREGKGDVFIAGGSDSFSSLAFSGFHALHALDENACSPFNHSTGITLGEG
                     SGILVIESYEHAVERGAKIYCEILGSGVSSDAYHITAPRPDGEGQMSAIRRAVESSAL
                     SFDDIDYINAHGTGTAKNDEAEFLSLHTLFDGNDHLSVSSTKSMTGHCLGAAGSIEAV
                     FSVKAIKENLVPPTIGYSDEDLKVLSEKAGNIDFIPNKSHTKDVHYAMSNSFAFGGNN
                     ASIIFSDNEHDIPDNSKNEKIYITGISKLTGTKTDEHSLNCNLTSEDYDKHGIKVAFY
                     RKLDRFSQLQLLSGMDALADADIKIDKDNEYKTGIVIGTADGPMTEIVDFQKKAITRG
                     TEKGSAFSFPNTVYNAAGGYLSIFSGIKGYNATVANGNQAGLQSICYAADILADGNES
                     VMLAAGTDENTKTNLELYTKAELLEKPLGEGSVTLVLETEESANGRNARKYAEIKGYA
                     QAHRPVSYDNSEVDSKAVVEVIEKACVNAGIEADNIDFVCCDDRKIIKTLMPCYDVSQ
                     EIGNARAAASAMTAAYAAELLSDSEKNYEYGLALSMGAGGTYSAVILKKA"
     gene            10959..11693
                     /locus_tag="PLS64_03505"
     CDS             10959..11693
                     /locus_tag="PLS64_03505"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HRL42929.1"
                     /translation="MSKTALVTGSSRGIGKACALKLAELGYDIAVNCNSNLEMAQKAV
                     DEITSLGRKAVAYKANTADIDEVKDMFRSIQQDFGGIDVLVNNAGVVDDAYLLMIKDE
                     SLERSLDINIKGYFNCARQASLKMLRKKSGIIINISSVSSVLAVEGQSVYSATKGAVN
                     AMTHTLAKELAKYGIRVNAVAPGFIETEMMNGIPEELQKKYLEAIPEKRFGTVQDVAN
                     VVGQLCSDDFSYMTGQVLVLDGGLSL"
     gene            11690..12145
                     /gene="fabZ"
                     /locus_tag="PLS64_03510"
     CDS             11690..12145
                     /gene="fabZ"
                     /locus_tag="PLS64_03510"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HRL42930.1"
                     /translation="MMNLLEINQRIKQRPPFQMIERVTELEPNVSATGIKNVSVNEPY
                     FAGHFPDTPIMPGVLLIESAAQLCSLVFAPESVEEDKLYVLLKVKDFKMLRPVIPGDT
                     LIISVNVTMSTKSAFEFACVIKVDGEVRAKGSLLFTTMDKNAVYKPSQE"
     gene            12148..>12813
                     /locus_tag="PLS64_03515"
     CDS             12148..>12813
                     /locus_tag="PLS64_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HRL42931.1"
                     /translation="MKVLVLNGSPKGEKSNTLNITKAFLDGFSADTEVEYITVKKETI
                     KPCMGCFSCWSRTPGKCVIKDDMQKIYEKINSADIIIESFPLYFFGMPSQMKALTDRC
                     LPYMLPYSGNVAEDKEASFHELRDEAMHEKRLAVITTCGYVSAEPMYPALIKQLDLIC
                     GNGNYTMIKCPEGELFIAEKAERQRKAYLDSITEAGREFCENRCLCDETMKKISKPIL
                     SPKG"
BASE COUNT         3717 a         2686 c         2792 g         3598 t
ORIGIN      
        1 tgtgaccctc tgcttgtaag gcagatgctc tcccagctga gctatgctcc cacattttta
       61 taatcggaaa gcactcccgt taagaagtgc tttccgtttt ggaggcgaca cccggatttg
      121 aaccggggcg tcagagtttt gcagactcgt gccttaccac ttggctatgt cgccgaatac
      181 tgagcaatgt cactcaacag gcattattct atcataataa aattcatttg tcaatactga
      241 atttgcattt taacaacaaa atttaacgga aaatttctac actgatattt tatgcatcaa
      301 acagaaaaat gacacctctg tttttgattc ctcataaagc cccgaaccct caaagatgca
      361 atactctgaa ggttcgggat tttggggtta tcatactttt cttaaaaaga gaagaaaaca
      421 agataagtat cttacacgct cggcttttca aatgccgaaa gaataagctt tacaccgtag
      481 attgacaaaa ggacaccaat cataaatccg attgtctgtg cggcaaatat aaggtggaaa
      541 aatccgtaga taccgccgat tatgacgagt attccgcaga aaagcggaat aagccatgca
      601 cctgaggaag atgctttttt gaccttgagt gacattgcaa cggacgagat accgtccact
      661 acaagccaac ctgagaaaat gcagagaatg ataacatcaa acataagtct tatgcccggc
      721 ataaacatag caagaattga aattactgcc gctgcaatgc cgacaacaag accgagtgtt
      781 cccattatcg cttcactctg ttcagccttt gcctttttgc gttttgcaaa gtagtcaatc
      841 acggagcata ttccccatac tccgagaaga attgtcacta tccaaccggt attaaggaag
      901 gtaagtcccg gatagaacat acaataaatc gcaccgatta tcgacaatat gccgaaaata
      961 caattaaata ctttcatagt cacctcatac tttaattagg gttcaataat attgaaatac
     1021 agcgggaatt cggcaacgct tgatgtaagg gttttgatga tgtcactgtt gccctcaacc
     1081 ttttcaatgc cggcgtccat aagtttctga ttgttctgtg caatgccgag aatacctgcg
     1141 ctcttggttt tgattgttgc gtcagcctta tcgctccact tttcctgtga atagataagc
     1201 acaccgtgat ttatgcgaag aagatatttt gcgtttttat cggtcacttc aagattgatt
     1261 acaaggttct gatcttcaag cttcttttcg tcaagacaga ttccgagata atccaacatt
     1321 gtttcggtac tcataccgag agctgttgca cctgaacctt cggaattcgg ataatttttt
     1381 gtaccgttac gaagctcata agcacctgta agataagcgt ttctccatgc accgctttca
     1441 gcctgatagc cgagctgttc gagtgcgtct gcgcaaagat agcgggcgtc tttattttcg
     1501 ggatctgcat atacaagtgt gtttgtaatc tgtgcaaccc actgatactc tcccttgtca
     1561 aagtcttttt tagccatttc aagaaccttg tctgtgtcgc cgagatactc tacaagcttc
     1621 tttgaatatt ctgtcggttc aagctcatca agatgaatgg ggttttcgtc ataccaaccc
     1681 atatacttct gataaaccgc ctttacattg tgcttgagag ttccgtaata ctggcgtgtg
     1741 taccaaacct tgttgagttc atctggaagc tccatcatat ttgcaatttc ggttgatgta
     1801 tagccctgat tgatgtacat aagtgtctgt gagaatatgt acttatagac agaggctgtg
     1861 tttgccatgt agtcattgat tacatcgttg ccccaatgag gccagttgtg agcctggaaa
     1921 actacttcgg ttttatctcc gaaaagctcc tttgcctcca taatgtactg cgcccacgca
     1981 ttgccgtcac gaacctctgc gcctctgagt gtgtagaggt tgtgcattgt gccggaacag
     2041 ttttcagcca tccacaaagc cttgtatttt ggaaggtagg tgttcatctc ggcaggcgnn
     2101 nnnnnnnnaa gctggaacac catttcaaca ccgtcaacct tgatggtttc ggtagctttc
     2161 tttacctcat aagacggtga aatgtatgtt gttgtgccgc ttgaaggggt aagaccgata
     2221 cctaccgaca aactgccctt accgccctta tcaagcaatg atccgtactg aaagttggtt
     2281 ctgcgtttca ttgcgtttcc tgcaaaaaca ttttcttcca taacagcctt ttcgtagcct
     2341 tcgggaacga taatgagcgt tttgccggac ttaatctgtt catcaagcgg aagtgatgaa
     2401 tctgcaacat tcttttcgtc aataagtccg ccgataccgc cgtagtggtc aatgtgagcg
     2461 tggcttacaa caacagcggc aatatgcgcc ttgccgaact tagattcaag cagttcaagc
     2521 gccgcctttg cagtttcgga gcttgttgta cagtcaaaaa caatccagcc gttgtcgctc
     2581 tttacaaagg ttacattggc aacatcgtag cctctgacct gataaacacc gtcaacaact
     2641 tcaaaaagtc cataaagcga gttaagctcc gtgtttctcc aaaggcttgg gtttgcactg
     2701 tcgggagttg acgagtttct gataaagtca tatgctgtct ggctccatgc aacagttccg
     2761 ttctcggttt tgatttcgag tttgtcgggt gctgttataa agcccttttt tgcaaattcc
     2821 ttttcctgtt catcggaaaa gtcaagggtc ttatacacct gtgcgttttc gtcagccgta
     2881 tattttgacg catcctttac atcgctgttg aattttgcgg atgtactgct gtcgctctgc
     2941 tgacatccgc aaagtgcaga tgaggcaatc aatgttgccg ccaaagcaag gcnnnnnnnn
     3001 nnaatcatag aatatttcct ccgtgttttt atctgtccat gattttttgg tttaagctga
     3061 attataataa ataattgtca tttagtccat acatttggca caaagtgaaa atttttgtca
     3121 cgaaatgcaa attaaagtca ctatttgaga tattatgtga tacaataaga gtgataatta
     3181 tgcaggaggt acggtatgag gattgcaata gttgatgatt gcgaaaatga gcgaaatgaa
     3241 ctttgcaaaa gactttctca aagctcattt tcaaggtcat atgatattga aatttgcgaa
     3301 tataacagcg gaacggattt tctgaatgag gcaatgcaga acagatttga ccttgttttt
     3361 atggatatat atatggaaaa ggaaaacgga attgatgcgg cacaaaagct gaggaagttt
     3421 gacaaagatt gtctgcttgt tttcacaacc acatcaaccg accacgcact tgaaggtttt
     3481 agggttcggg cattgcacta tcttgtgaag ccgtacagcg atgaagagct tgatatgttg
     3541 cttgatgaga tttcgcagaa aatttccgtt gaagaaaaat acattgaaat accctctgca
     3601 agtgactcgt tgcgtataaa gctttgcgac attttgtatg ccgagcattt taagcactgc
     3661 atacatattc attgtacgga caaatccgaa aagtctgtgc gcagtacatt tgccgaattt
     3721 gtaatgctgt ttgatgacgg ggataatttc tttgtgtgca accgaggaat catagtaaat
     3781 cttgaacaca tcagggatat gaacggcaat gaatttgttc ttgacaacgg tgaaagaatc
     3841 tccataagtc gaagtcttgt aaaaagcgca aaaagcacct ttggcgaata tattttcggc
     3901 aggagggatt tgcattgaca aatgttttct gtccgatgat tgaactcggt gcaatcatcc
     3961 ccggtgttgt acttgcatat cttccgataa aaaaacatct caaactatca aagctcaggc
     4021 tgtttcctgt gatgatttta tcacttgtgt gcctttgtac attgtgcggt tttgtttgtt
     4081 acagatacaa tctgaaatcg tttgtgcttg caataccgat agtgcttgct ctttcggcag
     4141 catactgttg ttcactcaaa acatccatgt ggaaatcggt gagtgtgtta cttgcggtat
     4201 gcggagtgtt tgcctgcatt gcgagcatta cacgggcggt tgattcgata acatttccgc
     4261 agaacacaac gccgtggttt gaaattcaat cggctgtgct gttcaatatt ctatgctgga
     4321 tgtttgtaat tcttgcatgg tatcctgcaa cacacggtgt gagtgaactg ctcgatgatg
     4381 aaaacattgc ccagacatgg tatgtattct ggattttacc gataatattc atcttgctca
     4441 acttttttat aatcccatgg aatcctaaca ttctccatac aggaagaatt atgcagggtt
     4501 acattgttat cagcgtatca cttcttgtgc tgttgctcat tttctacgct ttactgtatt
     4561 ttattgcaaa aagtctgaat cgcaataaca aacttgctca gaaaaatcaa tttttgaata
     4621 tgcagagggc acagtatgac gcattaaaag tcgctattga agaaacccgt caggcacggc
     4681 atgatatgcg acatcacttt gccgtactta atgctcttgc cgagcaaaag aactgggaag
     4741 aacttgaaaa atacctttcg tcagcatcgc agaatattcc cgacacagag cttgtactct
     4801 gtaaaaacca cgctgttaat gcgattatcg gacattatta ttcaaaatac aagagcaatg
     4861 gtattccgtt taaactaaaa attgacatcc ccgaaaagtt gacagtttcg gaatccgata
     4921 tctgtcttgt aatcgcaaat ttgcttgaaa atgcatttga ggcgagcatg aagcttgaca
     4981 gagataaacg gcagattaaa atgtatgccc gtccgcattc cgaaaagata ttgcttattt
     5041 ccgtagagaa tgctttcaac ggagagatta ccgaaaagga cggatttttc ggttcgtcaa
     5101 agcgaaacgg taacggtatc ggtacgcagt cggtacgcag aattgccgaa aaagacggcg
     5161 gttattgcag attttctcac gaaaacggaa tttttacggc ggatgttatg ctgcacggca
     5221 aaaactgaat cggacattat taaagggaag aaaccatttc ggtttcttcc ctgttttttt
     5281 attccgtaat tttattttct tttagcgaat attttgtcac cgtgttccaa tctcggcgga
     5341 ttgaaatatc agcggtaagg tttgtgttgt cataaacaat cgaccattga gtattgcttg
     5401 ttatatcgtt cggattcggt gcttgtgatg ccgaaacaag agcctcccat gctgtttctt
     5461 ttgtgtaact attattcttt tcaagaatat tgattatctt atcataccgc tctctgccgt
     5521 gaccgtaaac accgtttttc tgataaggct ttacaaaatt tttgtacatc acaaaaaagt
     5581 ttgtaacggc aggcgatttt gtaacaacaa gctttcggtc atcgctgttg caatcgtact
     5641 caataacacg accgtcaccg tttgaatcgg taacataaaa gtgataatct cttccgcttg
     5701 aggcgaacat atcgtatttt tcaaaaagtg taaccgcctc ttcggttgac gcagctctgt
     5761 caagtataag acggattgca agcgttgtgg cgataacagg ctttccgctt tgctgacgga
     5821 cgggcttgct gtcaagtgtg agaactgcga ttgacacgcc cttttcattg attccgtcaa
     5881 ggctgataaa cggtgagagc aaggttgcaa gccttgtttc atcactcaac tcggggttgt
     5941 ttgcacctac attatcaagt gccgcaaacg caaccgacct gtagccgtcc tttggcgaac
     6001 aatacacaag cattgacgag gtgtcgttct taaaatcata gtttcgcccc atcattacgc
     6061 tgtcgggatt tttcattgta aaggcactgc acccgaaatt cggcatttta agattgacgg
     6121 gataatcggg cagaacctcc tgcaaaattg caccgagagc cgactcatca tcaaaaatcc
     6181 ctctgtcaat gattttatca atgctgtaat cgtagcatat atccatttta taaaggttaa
     6241 aatcgctgta atcggtcagc tttttaacag aatcaagcgt atgctttctt gcgtcaatca
     6301 taatgtatcc tccttaaaac ggggtgtttt caacgcaata atagcataat atttttattt
     6361 tttcaacaat tttgagtcaa ttcgcaataa ttttttcata agaaaaatcg gaaagacaga
     6421 atttttgatt tctgtccttc cgacctgtat tttacatatt gaattttaaa ttatttaaga
     6481 agattattgt ctgtaaaata atcgattctc atagaatgac ggacatcctc aagtgttttt
     6541 gcggcagttt cctttgcaac cttactgccc tctttgagaa tatcgagaac atcgggaaga
     6601 tgattttccc attcttttct gcgttcacga ataggacgaa gctcctcctg cataacattg
     6661 ttaaggaatt tcttaacctt gacatcaccg agtccgccac gctgataatg agccttgagt
     6721 tcgtcaagac agctgtaatc attccaaaat tccgcaaaat actcgtcacg gcagaatgcg
     6781 tcaagataga taaacacggg attgccctca accttgccgg gatcctcaac acgaagatga
     6841 ttcgggtcgg taaacattga cataaccttt ttcttgatat cctcgggttc ttctgcaaga
     6901 taaatgcagt tgccgaggga tttgctcatc tttgccttgc cgtcaatacc gggcagtcgc
     6961 agacaagcct tgttgctcgg aagaataatc tgcggttccg taagagtttc gccgtaaacg
     7021 gaattgaact tgtgaacaat ctccttgcat tgttcaagca tcggaagctg atcctcgccg
     7081 acaggaacgg cagttgcacg gaatgccgtg atatcagccg cctgactgat tggataacag
     7141 aaaaatccca caggaatgct tgcctcaaaa ttacgcatct gaatttctgc cttaacggtg
     7201 gggttacgct gaacacggga cacggttacg aggttcatat aatagaaagt aagctctgta
     7261 agctcgggaa tcattgactg aacaaaaatt gttgacttgt tcggatctat tccgcaggca
     7321 aggtagtcga gcgcaacctg caaaatattc tgtcttacct tttcgggatg ctcggcattg
     7381 tcggtaagag cctgagcgtc tgcaatcata atgtatatat catcatattc gcccgaattc
     7441 tggagcttta ctctctcctt gagcgaacct gcatagtgac cgacatgaag tctgcccgta
     7501 ggacggtcac ctgttaaaat tacttttttc ataggtttct cctttgttta ccttgattca
     7561 aaggcaaaat tcttttctat gattatatca ccataaacaa cagccgtcaa catttgtaaa
     7621 aaagctaaaa agaaaaatat tccataataa ttcccatata ttttatgcca caaaattcga
     7681 cagaatgttg cataagacac acgcaaaatt catttgaccg ttgaattttt tttaaataaa
     7741 aatataatat tttaaaaaac gagggtaaag agtatggagt acaattatca taaaaatgca
     7801 ggacagcttg cattcataaa atttgtaaaa ggtcttgtgc atcttcttat tcgcccgaag
     7861 gttgaatgga aggacaagag ccttaaaaag agcctcaagg gcaagcctgt tgtgtttgta
     7921 tgcaaccaca ctcaccactt tgacggagtt gtgataagct ctgtactcag cagatacaag
     7981 ccgtatatgc ttgttaaaaa aagctggtat gacaagagcg ggacaggctc gtttatccga
     8041 atttgccgca gtattcccgt tgactttgac accatggaca caaactggta tgcacaaagt
     8101 gagggtgcgg ttgaaaacgg atattcaatg cttatcttcc ccgagggcgg aattgcaaga
     8161 gagggcaaaa tgctcccgtt caagcccggt gcggcattgc ttgcggcaaa aaaaggtctt
     8221 gacataatcc cgattgcatc tttgggtgag tataaaattc tttttggaaa gaggcagaaa
     8281 attttaatcg gcaatccgat aaaatccgaa tgtcccgaaa atgttcgcta ctcaaaatat
     8341 gcaaaagaaa taagtgccga agccgaaaag caggtcaaaa agctttatct tgagcttgaa
     8401 gaaaaatacg gcaaaggcaa ggtttattac aaagaagatt tttcttcgga aaatttataa
     8461 taaaatcaaa aaaatcaaaa ttttaagtga ggtaattaaa atgaacaatg ctattgcaga
     8521 tcagattaag gaaatgctcg tagaaaacct tagaattccc gaaaacattc ttacatacga
     8581 ttcagaactt ttcggtgacg aaatcggtct tgactcaatc gactcaatcg aaatcgttgc
     8641 aggcatcgac acacttttcg gtattgatat gacaggtgca gaccgtgagc acttccaatc
     8701 aatcagagca cttacagaat atgtagaaag caagcagggt taatatggct gacaaaaaga
     8761 gatgtgttgt cacaggactc ggacttatct gcggcgtcgg tgacaatgta aaggattgct
     8821 gggatgccgt aacactcggt cactccggca ttgatgaggt taagtcggtt gacacctcaa
     8881 actgttatgc acacaagggt gcagaggttg acaaggcatc gtcagagctt tctcccgaaa
     8941 actatgaccg ttcttccctt ctctgtattc atgccgcaaa cgaggctttt gaagatgcaa
     9001 acattgacgc atccgaaaag aacatcggtg ttattcttgg cagttgtgta ggcggtgcgg
     9061 caagcattga caaatattac acagacgaaa tcaagggtga cggcggtaaa aaagaagata
     9121 ttttcaaaat gggtgcttcg gcaattgcca acaatgtttc cgctcatttc ggacttgagg
     9181 gcattaccgc aaacatcgtc aacgcttgtg cggcaggcac aatgagcatc ggctatgcct
     9241 gtgaccttat ccgtgagggc aagggtgatg tgttcatcgc aggcggttcg gacagctttt
     9301 cttcacttgc attcagcggt ttccacgctc tccatgcgct tgacgaaaac gcctgttccc
     9361 cgttcaatca cagcacaggt atcacgctcg gcgagggttc gggtattctt gtaatcgaaa
     9421 gctacgaaca cgctgttgaa cgaggcgcaa agatttactg tgaaattctc ggttcgggtg
     9481 taagcagtga cgcttaccac attaccgctc cccgtcccga cggtgaaggt cagatgagtg
     9541 caatcagaag ggctgttgaa agctctgctc ttagttttga cgatatcgac tacataaacg
     9601 cacacggaac tggtactgcc aaaaatgacg aggctgaatt tctttcactt cacacacttt
     9661 ttgacggcaa cgaccacctt tctgtcagct caacaaaatc tatgacaggt cattgcctcg
     9721 gtgcggccgg ctcaattgag gctgtatttt ccgtaaaagc aataaaagaa aacctcgttc
     9781 cgcctacaat cggctactcc gatgaggatt tgaaggtgct ttccgaaaag gcgggcaaca
     9841 ttgactttat tccgaacaag agccacacaa aagatgttca ctatgcaatg tcaaactcat
     9901 ttgcattcgg cggcaacaac gcaagcatca ttttctcgga caacgagcat gatattcccg
     9961 acaacagtaa aaatgaaaaa atttacatta cgggcatttc aaagcttaca ggcacaaaga
    10021 ctgacgaaca cagccttaac tgcaacctta cctccgaaga ttatgacaaa cacggaatca
    10081 aggttgcatt ttacagaaag cttgaccgtt tcagccagct ccagcttttg agcggtatgg
    10141 acgcacttgc agacgctgac attaaaattg acaaagacaa cgaatataaa acaggtattg
    10201 taatcggcac ggctgacggc cctatgaccg aaattgttga tttccagaaa aaggcaatca
    10261 caagaggtac cgaaaagggc agtgcttttt cattcccgaa tacggtttac aatgccgcag
    10321 gcggttatct gagcattttc tcaggcatca agggctacaa cgcaactgtt gcaaacggca
    10381 atcaggcagg actgcagagt atttgctatg ccgctgatat tcttgcagac ggcaacgaaa
    10441 gcgttatgct tgccgcaggt accgatgaaa atacaaagac aaaccttgaa ctctatacaa
    10501 aggccgaatt gcttgaaaaa ccgctcggtg agggctctgt aactctcgtt cttgaaacag
    10561 aggaaagtgc aaacggcaga aatgcacgca agtatgcaga aattaagggc tatgcacagg
    10621 ctcaccgccc tgtatcttat gacaactcag aggttgacag caaggctgtt gttgaggtta
    10681 tcgaaaaggc ttgtgtgaat gccggcattg aggcagacaa tatcgacttt gtatgctgtg
    10741 acgacaggaa gattatcaag acgcttatgc catgctatga tgtatcgcag gaaatcggca
    10801 atgcaagagc cgcagcaagc gcaatgacag cggcatatgc ggctgagctt ctttctgaca
    10861 gcgagaaaaa ttacgaatac ggacttgctc tttcaatggg tgcgggcgga acatactctg
    10921 ccgtaatcct gaagaaagcg taaaaatcgg aggtaaatat gagcaaaacc gctcttgtaa
    10981 caggttcatc aaggggcatt ggcaaggctt gcgctttaaa gcttgcagag cttggatatg
    11041 acattgccgt gaactgcaat tcaaatttag aaatggctca aaaggctgtt gacgaaatta
    11101 catcactcgg cagaaaagcc gttgcctaca aggcaaacac ggcagatatt gacgaagtaa
    11161 aggatatgtt ccgcagtatt cagcaggact tcggcggaat tgatgtgctt gtcaacaacg
    11221 caggtgtggt tgacgacgct taccttttga tgattaaaga cgagtcgctt gaaagaagtc
    11281 ttgacattaa tatcaaagga tatttcaatt gtgcaagaca ggcatcgctt aaaatgctcc
    11341 gcaaaaagag cggaatcatc atcaacattt cttccgtaag ctctgttctt gcagttgaag
    11401 gtcagtcggt ttacagtgca acaaagggtg cggtaaacgc tatgactcac acgcttgcaa
    11461 aggaacttgc aaagtacgga atcagagtaa acgcagttgc acccggtttt atcgaaaccg
    11521 aaatgatgaa cggcattccc gaagaattgc agaaaaagta tcttgaggct attcccgaaa
    11581 agagattcgg cacagttcag gatgtcgcaa atgtcgttgg acagctttgc agtgacgatt
    11641 tttcatatat gacaggtcag gtgcttgtgc ttgacggagg actcagctta tgatgaatct
    11701 gcttgaaatc aatcaaagaa taaaacaaag accacccttt cagatgattg agagggtgac
    11761 cgagcttgag ccgaatgttt cggcaacagg tataaaaaat gtgtcggtga acgagccgta
    11821 ttttgcaggt cattttcccg acacaccgat tatgccggga gtattgctca ttgaatctgc
    11881 ggcacagctt tgcagtcttg tatttgctcc cgaaagcgtt gaggaggaca agctgtatgt
    11941 tcttctaaaa gtcaaggatt tcaaaatgct ccgccccgtt attccgggtg acacgcttat
    12001 tatcagcgta aatgttacaa tgtcaacaaa gtcggcattt gaatttgcgt gtgtcatcaa
    12061 ggttgacggt gaagtgaggg caaagggaag tcttttgttt accacaatgg ataaaaatgc
    12121 ggtttacaag ccgtcacagg agtgattatg aaggttctcg tattaaacgg tagtccaaag
    12181 ggcgaaaaaa gcaatactct caacattaca aaagcatttc ttgacggctt ttcagccgat
    12241 acggaagttg aatatatcac ggttaaaaag gaaacaatca agccgtgtat gggttgcttt
    12301 tcgtgttgga gcagaacacc cggcaaatgt gttataaagg acgatatgca gaaaatctac
    12361 gaaaagatta attctgccga tattatcatt gagagctttc cgctctactt tttcggtatg
    12421 ccgtcacaga tgaaagcatt aaccgacaga tgtctgccgt atatgctccc ctattcggga
    12481 aatgttgccg aggacaaaga agcctctttc catgaactgc gtgatgaagc aatgcacgaa
    12541 aagaggcttg cggttattac aacttgcggc tatgtgagtg ccgaaccgat gtatcccgca
    12601 cttataaaac agcttgattt gatttgcggt aacggcaatt acacgatgat aaaatgcccc
    12661 gagggcgagc tttttattgc agaaaaagct gaaaggcaga gaaaggcata ccttgattca
    12721 atcacggagg caggcaggga attctgcgaa aaccgctgcc tttgcgatga aacaatgaaa
    12781 aaaatttcaa agcctattct ctcccccaaa gga
//
LOCUS       DAOMVE010000039        12679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1126_length_12679_cov_3.406290, whole genome shotgun sequence.
ACCESSION   DAOMVE010000039 DAOMVE010000000
VERSION     DAOMVE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12679
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1126_length_12679_cov_3.406290"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="PLS64_03520"
     CDS             complement(<1..132)
                     /locus_tag="PLS64_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRL42932.1"
                     /translation="MAYSEKVMDHFANPRNVGEIENADGIGEVGNSKCGDIMKMYIKV
                     "
     gene            complement(202..1389)
                     /gene="nifS"
                     /locus_tag="PLS64_03525"
     CDS             complement(202..1389)
                     /gene="nifS"
                     /locus_tag="PLS64_03525"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HRL42933.1"
                     /translation="MRYVYADNSATTALSPKVLEKMMPYLTEVYGNPSSIYTIGAKAR
                     AAVETARENIAKNLGAANPNEIYFTSGGSESDNWALKGVCHALKAKGKKHIITSKFEH
                     HAILHTCEALEKEGFEVTYLDVYENGIVHPEDVENAIREDTAIVSIMYANNEIGTIQP
                     ISEIGAICRKHGVLFHTDAVQAAGYVKIDVQKENIDLLSMTAHKLHGPKGCGLLYIRK
                     GVRIENLICGGAQERNKRAGTENVAGIVGLDAALQLAVDGMDERNAKLEKMRDRLIDG
                     LLKIERSRINGDRVHRLPGNVNMCFEGIEGESLLLRLDLKGVSASSGSACTSGSLDPS
                     HVLLAIGLPHEIAHGSMRLSFSDDNTEEDIDYILEIVPEIVSYLRSMSPLWEKIKKQE
                     AQG"
     gene            complement(1419..1856)
                     /gene="rnhA"
                     /locus_tag="PLS64_03530"
     CDS             complement(1419..1856)
                     /gene="rnhA"
                     /locus_tag="PLS64_03530"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRL42934.1"
                     /translation="MKQVEIFTDGACSGNPGPGGWGAVLRYNGHEKEISGGEANTTNN
                     RMELSAVINALALLKEPCKVTLYSDSQYVCNALKLGWAKKWKANNWMRNKKEKALNPE
                     LWDRLLTLYDTHEVEIVWVKGHAGHPENERCDRLAVAAAKQFA"
     gene            2413..4287
                     /locus_tag="PLS64_03535"
     CDS             2413..4287
                     /locus_tag="PLS64_03535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRL42935.1"
                     /translation="MLKQSRYSKGKKNVKQYSKNNASKKSGSGKNSYNAYYKKNDDGF
                     QPKSNGKSNYGKKKYTSKSKGYPKKNAQKPSIKIAFLGGLNEIGKNITLVECENDIII
                     IDCGMAFPDGDMLGVDLVIPDFTYIEQNADRVKGIILTHGHEDHIGGLPFLLSKVNVP
                     IYGTPLTLGLVGNKLKEHSLYNQSQLNVINAGQSIKLGCMKIDFIHVNHSIPDAVAFA
                     IHTPGGTIVHTGDFKIDCTPITGDMIDLSSFARVGDEGVLALLSESTNAERPGYTPTE
                     RTVSDSLDNLFKSAENYRIIIATFASNVNRVQQIIDCAEKYGRKVAFSGRSMVNYMDV
                     AQKLGYLHLPENILIDIDMLDKYMPQQVVLVTTGSQGEPMSALSRMAYSDHRKVMVGE
                     GDFIIISANPIPGNEKTVGNVVDELLKKGCKVIYESMYEVHVSGHACQEELKIMQKLV
                     KPKYFIPVHGEQKHLRKHADLAMFLGMERKNIYIGDVGSVLEINEDYMKELPPVQAGK
                     VFVDGLGVGDVGSIVLRDRKHLGQDGLIIIVASLDVYDGHVISGPDIVSRGFVYVRES
                     ERLLEEVKQTALAILEDCAENNVHEWGIIKNRIKDDVSKLIGQRTRRAPMILPILQEV
                     "
     gene            4340..6295
                     /locus_tag="PLS64_03540"
     CDS             4340..6295
                     /locus_tag="PLS64_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRL42936.1"
                     /translation="MRKRNWTLTPWFIIFSVIMFAMAVVSYDYSPVVFYIELGVSVLS
                     LAVVLITSLRFTSYVKSTVKNIVGSIKGINEDYLEKFICPVAVVGRQGDIVWCNSRFR
                     KAMCGGKGAEGDNIKAYLSGKEIDCLKKGEGCDVAIDGREYTVYCRSTGEGAVCEFIE
                     NTYYKQITREYLASKPCVAIAVFDNAEDFSDNSDESFSEAMLDIEVCLQKWAEQYSAL
                     YKKIGNNRYMIIFRDADVEKMAADKFPILKTIRGITINNHSASISVGLCRGYNNIKES
                     ELNARKALEMALGRGGDQVAVIKKDNTYEFFGGKVAAAEKASKVRMRVIANAISRVVA
                     DCDKVFIMGHKFSDLDCVGAAIGLQCIMEKTFKRYSKVVINRETSMAKQLIEYTDEKL
                     DTDIFISPEAALSGLTQKSLLIIVDTHLKRSLESTELYEKCKKVIIIDHHRKAVDYIN
                     NALVFCHEPSASSTCEMCSEIISCLDDSPLTYVQADAMLAGITLDTKNFAVKTGVRTF
                     EAAAYLRKRGANTLTVKGMFSDNIETYREKVDIVCKAHVYRGCAISIAPSGDGDIRLA
                     SAQAADEMLNLKGVDASFVLFEDNSQINISARSYGNVNVQIIMEKLGGGGHQTMAATQ
                     LKNTTKELAYQQLLSEIDNTLDDEENS"
     gene            6312..6758
                     /gene="rplI"
                     /locus_tag="PLS64_03545"
     CDS             6312..6758
                     /gene="rplI"
                     /locus_tag="PLS64_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRL42937.1"
                     /translation="MKVILMQDVKSLGKKGELVEASDGYARNFLLPRKLAKEANAQAM
                     NEYKNAENSKNYKIATQKAQAEHDKKTLEGKTFKMTAKAGQGGRLFGSVTSKQVAEEI
                     KKQYNIDVDKRKVVLDCDIKEFGTYHAEVKLFTGVSAKIDVQVSEA"
     gene            6774..8147
                     /gene="dnaB"
                     /locus_tag="PLS64_03550"
     CDS             6774..8147
                     /gene="dnaB"
                     /locus_tag="PLS64_03550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRL42938.1"
                     /translation="MADLNFAGSYDGLHLPYSPEAEQAVLGAIILESDTFDKVVDTLK
                     SPDYFYVSLHKLIFAQMQEMIGLGLSIDFVTLLEKLKQNKAFDETTGKTYLMDLVNNC
                     PSISNAEEYAKIIAEKYNVRRLITASREIIDDATAGNDDPSVLIDSAEQKIFDIRQGN
                     EKHGLERINSVILQTFDRLDALNSETDNSMKPIPTGIGDLDRMITGLNRSDLIILAAR
                     PGMGKTSFALNIARNVACKSKKTVAFFSLEMSKEQLASRLLSSEALIGGTKLRTGQLT
                     EEEWSRLIPASDILSKTDLYLDDTPGITIPEMKSRLRRLHNLDLVVIDYLQLMSSSRK
                     IDSRVNEISEITRNLKILAKEMNVPVITLSQLSRAAEKRDDHRPQIADLRDSGSIEQD
                     ADIVLFLYREGYYDKEGGEDSAAPTADMNSGECIVAKNRHGETNIVKLHWQGEFMRFT
                     GTDNRDA"
     gene            8140..9468
                     /gene="tilS"
                     /locus_tag="PLS64_03555"
     CDS             8140..9468
                     /gene="tilS"
                     /locus_tag="PLS64_03555"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRL42939.1"
                     /translation="MLNRVTATVEKYGLLEKGDSVIVALSGGADSTALLSVFVSLKEK
                     YNLSIYAAHINHNIRGDEAKRDENFCKILCKKFNTELFIKSVDVPTLAKQQKISEELC
                     GRNVRYAFFEELSQKLCAKIATAHTASDNAETLIFNIARGASLTGLSAIPPKRGNIIR
                     PLIELSRGDIESYCEENNLEYVTDSTNLADDYTRNYIRHNIIPCLKKLNPSFERTALG
                     LSENAGESADFIKKCAEKAVHDCKSDFGYDCKKLLSLDSAVLKEMLFILLKNNCNISP
                     ERKTILLLCEIIRNGGSVELSKQCTAVSKQGILRFVCPKNSPDFNEIPLKNNMTFSYD
                     GKIYTVNEITEKVTNQNNLVSKKRLGENPVFRTRRAGDRFTYPDRMVTKPLRKVFNEQ
                     KIPSEMRDRLLLLAVSNTVLWCEKLGVSLQGKADDTEKTEKLLINIDKEG"
     gene            9471..10007
                     /gene="hpt"
                     /locus_tag="PLS64_03560"
     CDS             9471..10007
                     /gene="hpt"
                     /locus_tag="PLS64_03560"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRL42940.1"
                     /translation="MHKDIEKILYSEKDIENIVNTIAKQIEKDYNDKDFIMVGLLKGS
                     VAFMVDLMRKVNLDFSIDFIVASSYGSGTVSSGRVNIQKDISQSVEGKDILIVEDIID
                     SGNTLDFITKYLKAKKAKSVKLCTLFNKPDRRVADIHIDYAGAVIPDEFIVGYGLDYD
                     EKYRNLPYVGVLKPSVYS"
     gene            10094..12109
                     /gene="ftsH"
                     /locus_tag="PLS64_03565"
     CDS             10094..12109
                     /gene="ftsH"
                     /locus_tag="PLS64_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRL42941.1"
                     /translation="MDNKKKVRNLLLYLGIPILIIIITAAVLSTNKSTSPKTSELEQY
                     FVQDMVDSFNIDYGSGQIEITLKEGMSPIKSTDSDSQSATTATQPATEQQKTQSNSKK
                     SKIVVKGQLADIQRFLDDIKPYYNPASTDEIPHNLTRATDNSILMEMIPTLIIMIILI
                     VAWVLIMKKMGGGGLGGKEMSFGKAKIKNTNDEKRKTTFDDVAGADEEKEELAEVVEF
                     LKAPEKYNKLGARIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMFVGV
                     GASRVRDLFDQAKKNSPCIIFIDEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFG
                     ANEGVILIAATNRPDVLDPALMRPGRFDRQVVVSYPDVNGREAILRVHARKKPLAPDV
                     NLKTIAKTTAGFTGADLENLLNEAALLAARKDKKAITMDEIKEATVKVVVGAEKKSKV
                     MSEKEKKLTAYHEAGHAILFDKLETQDPVHEISIIPTGMAGGYTMPLPSEDKSYNSRR
                     GMYEDIVVSLGGRVAEELIMDDISTGASNDIEKATKTARAMVTKYGMTKELGCVCYGT
                     DNNSVFLGRDMGSRTQDYSEATAAKIDQLVLDMVNKAYGDATKILSDNMDKLHDIAKY
                     LIKHEKMGSEDFTAVMNGTYKEPEEVEQETADKEPSAETKVDESTEG"
     gene            12222..>12679
                     /locus_tag="PLS64_03570"
     CDS             12222..>12679
                     /locus_tag="PLS64_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRL42942.1"
                     /translation="MAQDKIIVRGAKEHNLKNVNVEIPRNKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARMFLGQMDKPNVESIEGLSPAISIDQKTTSKNPRSTVGT
                     VTEIYDYLRLLYARIGVPHCTVCGREIRQQTVDQIVDKIMTYETGTKIQI"
BASE COUNT         3808 a         2583 c         2848 g         3440 t
ORIGIN      
        1 aaccttaatg tacattttca taatatcgcc gcattttgag ttaccgacct cgccaatgcc
       61 gtctgcgttt tcaatttcgc ctacatttct cggattagcg aaatgatcca ttactttttc
      121 cgaataagcc attttaatcc tcctgaatat attgttgccg aatatctatt caattctata
      181 cgaattttaa cgggttgtca atcaaccctg agcttcctgc tttttaatct tttcccaaag
      241 aggtgacata cttctgaggt acgaaacaat ttcgggaacg atttcaagaa tataatcaat
      301 atcttcctct gtattgtcgt cagagaatga aagacgcata ctgccgtgag caatttcgtg
      361 gggaagaccg attgcaagaa gtacatgact cggatcaaga ctgcccgatg tacaagcaga
      421 acccgatgat gccgaaacac ccttgaggtc aagtcttaaa agaaggcttt caccctcaat
      481 accctcaaag cacatattga cattgccggg gagtctgtga actctgtcac cgttaattct
      541 tgaacgctca atctttaaaa gaccgtcaat aagtctgtca cgcatctttt caagctttgc
      601 gtttctttcg tccataccat caactgcaag ctgaagtgcg gcatcaagac cgacaatacc
      661 tgcaacattt tcggtaccgg cacgcttgtt cctctcctga gcaccgccgc agatgaggtt
      721 ttcaattctt acgccttttc tgatgtagag aagaccgcag cccttcggtc cgtggagctt
      781 gtgtgctgtc attgaaagaa ggtcaatgtt ttctttctga acatcaatct tcacataacc
      841 tgccgcctga actgcgtctg tatggaaaag aacgccgtgc tttctgcaaa ttgcaccgat
      901 ttctgaaatc ggctgaattg taccgatttc gttgtttgca tacataatcg aaactatagc
      961 cgtgtcctca cgaattgcat tttcaacatc ctcgggatga acgataccgt tttcgtaaac
     1021 atcaagataa gtaacctcaa agccttcctt ttcaagagcc tcacatgtgt gaagaatagc
     1081 gtgatgctca aactttgaag taatgatgtg ctttttgccc tttgccttga gagcgtgaca
     1141 aacacccttg agcgcccagt tgtcggattc acttccgcct gaggtaaagt aaatttcatt
     1201 cgggttagcc gcaccgagat tctttgcaat gttttctctt gcagtttcta cggcagccct
     1261 tgcctttgca ccgattgtat aaatgcttga cgggtttccg taaacctcgg tgaggtatgg
     1321 catcattttt tcaagcacct tcggcgaaag tgctgtagta gccgagttgt cggcataaac
     1381 atatctcatt ttatatccac ctttatataa gtttattgtt atgcaaattg ttttgcggct
     1441 gcaacggcaa gtcggtcgca acgctcgttt tcgggatgac ctgcgtgtcc ttttacccac
     1501 acaatttcaa cctcgtgagt atcatacagg gtaagaagcc tgtcccaaag ttcggggttc
     1561 aaagcctttt cttttttatt acgcatccag ttgtttgctt tccacttttt agcccaaccg
     1621 agttttagtg cgttgcaaac atactgcgag tcgctgtaaa gcgttacctt gcacggttct
     1681 ttgagcagag caagtgcatt gataacagcc gaaagctcca tacggttgtt ggttgtgttt
     1741 gcctcaccgc ctgaaatttc cttttcgtga ccgttatatc ttaaaacagc accccaaccg
     1801 ccgggaccgg gatttccgct gcaagcaccg tctgtaaaaa tttcaacttg tttcaaaaat
     1861 aattctcctt taattcagag tgtactaatt attcaaaata ttaaaatccg cactatagat
     1921 tataatacgc tgaaacgaca attgcaaccc attatttgat ataattctga taactttggt
     1981 aggtgtttga acaaattttg aaatttatac ataatattgc aattaaaaaa tcgcttacgg
     2041 gttttatttt acggaaattt caaattttta tgcataaaat tgcctttgtt atgcggattg
     2101 caagccttgg attttgtgtt tgttatgaaa atttcttgac attgcagtag gatatgcggt
     2161 aaaataaaat gaaaaactat gatagggtag ctatgtgttg ccataattac gggaataaaa
     2221 ttcgtatatt ttgcggatat tattataaat acagatgttc accgttatca gaacggagaa
     2281 tttattttat aaataaatta agacctcacg ggcgctcaga ggtctttgat atatttattt
     2341 ttcttttatt cagtacatat ttcagaaaac ggaggtagat ttgtgagtac agaaaagagg
     2401 aacgaaaact ttatgcttaa acagtcaagg tactcaaaag gcaaaaagaa tgtgaagcaa
     2461 tactctaaaa ataatgcttc aaaaaagagc ggttcgggta aaaattccta caacgcttac
     2521 tataagaaaa atgatgacgg atttcagccg aaatcgaacg gaaagtcaaa ttacggcaaa
     2581 aagaaatata cgtcaaagtc aaagggttat cccaaaaaga atgctcagaa gccgtcaatt
     2641 aagattgcat ttctcggcgg ccttaacgaa ataggcaaga acatcaccct tgttgaatgt
     2701 gaaaacgata taatcatcat cgactgcggt atggcatttc ctgacgggga tatgctcggc
     2761 gttgaccttg taattccgga tttcacatat attgaacaga atgccgacag agttaagggt
     2821 attatcctta cacacggaca tgaggatcac atcggtggtt tgccttttct tctttcaaag
     2881 gtgaatgttc cgatttacgg tacaccgctt acgctcggtc ttgtaggtaa caagctcaag
     2941 gagcatagtc tttacaatca atctcagctt aatgtaataa atgcaggtca gtcaataaag
     3001 ctcggttgta tgaagattga cttcattcat gtaaaccatt cgattcccga tgctgttgct
     3061 tttgcaattc acactccggg cggtactatt gttcacacgg gcgacttcaa gattgactgc
     3121 acacctatca cgggtgatat gattgacctt tcaagctttg caagagtggg tgacgaaggt
     3181 gttcttgcac ttctttcgga gagtacaaat gccgaaagac ccggatacac ccctacggaa
     3241 agaactgtat ccgacagcct tgacaatctt ttcaagagtg cggaaaatta cagaattata
     3301 attgcaactt ttgcgtcaaa tgtaaaccgt gttcagcaga ttattgactg tgcagaaaag
     3361 tacggaagaa aggttgcttt ttcgggcaga agtatggtca actatatgga tgttgcacaa
     3421 aagctcggat atcttcatct tcccgaaaat attcttatcg atattgatat gctcgacaag
     3481 tatatgccgc agcaggttgt tcttgttaca acaggcagtc agggcgagcc gatgagcgca
     3541 ctttcaagaa tggcatattc agaccaccgc aaggtaatgg tcggtgaggg tgatttcatc
     3601 ataatttcag ccaatccgat tcccggtaat gagaaaacag tcggcaatgt tgttgacgaa
     3661 cttcttaaaa agggttgtaa ggtaatttat gagtcaatgt atgaagttca tgtttcaggt
     3721 catgcctgtc aggaggaact caaaatcatg cagaagcttg taaagccaaa gtattttatc
     3781 cctgttcacg gcgaacagaa acacctcaga aaacatgccg accttgcaat gtttctcggt
     3841 atggaacgca agaacattta tatcggtgat gtcggaagtg ttcttgaaat caacgaggat
     3901 tatatgaaag agcttccgcc tgttcaggca ggcaaggtgt tcgttgacgg tcttggcgtt
     3961 ggtgatgtcg gaagtatcgt tcttcgtgac agaaagcacc tcggacagga cggacttatc
     4021 attattgttg cttctcttga tgtttatgac ggtcatgtta tcagcggccc cgatattgtt
     4081 tcaagaggat ttgtatatgt tcgtgagagc gaaagacttc ttgaagaggt caagcagacc
     4141 gctcttgcaa ttcttgagga ttgtgcagag aataatgttc acgaatgggg cattattaag
     4201 aacagaatca aggatgatgt ttcaaaactt atcggtcagc gtacaaggag agctccgatg
     4261 attcttccta ttcttcagga ggtttaagtt ttattcgctg atttattttt tgctattttg
     4321 ttattaacag aggtttgaca tgagaaaaag aaattggacg cttacaccgt ggttcataat
     4381 tttttcggtg ataatgtttg ccatggctgt tgtcagctat gactattcgc ctgttgtgtt
     4441 ctatatcgaa ctcggtgtgt ctgttctttc cttagccgtt gtgcttataa cttcattgcg
     4501 atttacaagc tatgtaaaaa gtacggtaaa aaatatagtc ggaagtataa agggtattaa
     4561 cgaagattac cttgaaaaat tcatttgtcc tgttgccgtt gtaggaagac agggtgacat
     4621 tgtatggtgc aattcacgat tccgcaaggc tatgtgcggc ggaaagggcg cagagggcga
     4681 taatatcaaa gcctaccttt cgggaaaaga aattgactgt ctgaaaaagg gtgagggctg
     4741 tgatgttgcc attgacggca gagaatacac ggtttattgc cgttctacag gcgagggcgc
     4801 tgtctgtgaa tttatcgaaa acacctatta caagcagatt acaagggaat atcttgcttc
     4861 caagccgtgc gttgcgattg ccgtttttga taatgcagag gacttttccg ataactcgga
     4921 cgagtcgttt tccgaggcaa tgctcgatat tgaagtttgc ctgcaaaagt gggctgagca
     4981 gtattcggct ctttacaaaa aaatcggcaa caaccgttat atgataattt tcagagatgc
     5041 cgatgttgaa aaaatggcag ccgataagtt tccgattctt aaaactatcc gaggaattac
     5101 gataaacaac cacagtgcgt caatctcggt aggtctttgc cgaggttata ataacatcaa
     5161 agagagcgag ctcaacgcaa ggaaagccct tgaaatggca ctgggcagag gcggcgatca
     5221 ggttgctgtc attaaaaaag acaatacata cgagttcttc ggcggaaagg ttgccgcagc
     5281 ggaaaaggca agcaaggtgc gtatgagagt tattgcaaac gcaatcagcc gtgttgttgc
     5341 cgactgcgac aaggtcttta ttatgggaca caagttttcc gaccttgact gcgtaggtgc
     5401 ggcaatcgga cttcaatgca taatggagaa aactttcaag cgttattcaa aggttgtcat
     5461 taaccgagaa acctcaatgg caaaacagct cattgaatat actgatgaaa aactcgacac
     5521 cgatatattc atttctccgg aagccgctct ttcgggactt acccagaagt cgcttcttat
     5581 catagttgat acgcacctga agcgttctct tgaaagtacg gagctctacg aaaaatgtaa
     5641 aaaggttatc attatcgacc accacagaaa ggcggttgat tacataaaca acgctcttgt
     5701 gttctgtcac gaaccctcgg cgtcttcaac ctgtgaaatg tgcagtgaaa taatcagctg
     5761 tcttgatgat tctccgctca catatgttca ggcagacgca atgcttgcag gcattacact
     5821 tgacacaaag aattttgccg ttaaaacagg cgtgcgcaca tttgaggcgg cggcttatct
     5881 gagaaaaagg ggcgcaaaca cactcaccgt aaagggtatg ttctccgata acatcgaaac
     5941 ctaccgtgaa aaggttgaca ttgtgtgcaa ggctcatgtt taccgtggct gtgcaatttc
     6001 gattgcgccg agcggtgacg gcgatataag gcttgcgtcg gctcaggcgg ctgatgaaat
     6061 gctcaacctc aaaggtgttg acgcatcctt cgttcttttt gaagacaaca gtcaaattaa
     6121 catttcagca agaagctacg gtaatgttaa tgttcagatt ataatggaaa agcttggcgg
     6181 cggcggtcat cagaccatgg cggcgacaca gcttaaaaac actacaaaag agcttgctta
     6241 tcaacagttg ctgtctgaaa ttgacaacac acttgatgat gaggaaaact cgtaagaaag
     6301 gttgattggt tatgaaagta attttaatgc aggatgtaaa atcactcggc aaaaaaggcg
     6361 agcttgttga agcgtctgac ggctatgcaa gaaactttct ccttccgaga aagcttgcaa
     6421 aagaggcaaa cgctcaggct atgaatgaat ataagaatgc cgagaattcc aaaaattata
     6481 aaattgcaac ccaaaaggct caggcagaac atgacaaaaa gactcttgag ggcaaaacct
     6541 tcaagatgac tgcaaaagca ggacagggcg gcagactttt cggcagcgta acttcaaagc
     6601 aggttgccga ggaaatcaaa aagcagtaca acattgatgt tgacaagcgc aaggttgttc
     6661 ttgactgcga cattaaggaa ttcggtacat atcacgcaga ggtaaagctc tttacaggcg
     6721 tttctgcaaa gattgatgtt caggtatccg aggcataata aacaggagta aaaatggctg
     6781 atttaaattt tgcgggcagt tatgacggac ttcatctgcc gtacagcccc gaagcggaac
     6841 aagcggtttt aggtgcaatc attcttgaaa gcgacacctt tgacaaggtt gttgatactc
     6901 tgaaaagccc cgattatttc tatgtttctt tgcacaagct gatttttgca caaatgcagg
     6961 agatgatcgg actcggcctt agcatcgact ttgtcactct gcttgaaaaa ttaaaacaga
     7021 ataaggcttt tgacgagaca acgggaaaaa cctaccttat ggatcttgtc aacaattgtc
     7081 cgtcaatttc aaatgccgag gagtatgcga agattattgc ggaaaagtac aatgttcgca
     7141 gacttattac ggcgtcccga gaaattattg atgacgcaac tgcaggcaac gatgatccct
     7201 cggttctgat tgattcggca gaacagaaga tttttgacat cagacagggc aatgaaaagc
     7261 acggtcttga gcgtatcaac tcggtaattt tacagacttt tgaccgactt gacgcactca
     7321 acagcgaaac cgacaacagt atgaaaccta tcccaacggg cataggcgac cttgaccgaa
     7381 tgattacggg acttaaccgt tccgacctta tcattcttgc cgcccgaccg ggtatgggta
     7441 aaacgagttt cgcactcaat attgcacgaa atgttgcgtg taagtctaaa aagaccgttg
     7501 cgtttttctc gcttgaaatg tcaaaggaac agcttgcaag ccgtctgctt tcaagtgagg
     7561 cgcttatagg cggtacaaaa ctgagaacag gtcagcttac cgaggaggag tggtcaagac
     7621 ttatccccgc aagcgatatt ctcagcaaaa ccgacctcta tcttgacgat actccgggta
     7681 ttacaattcc tgagatgaag tcaagacttc gccgacttca caaccttgac ctcgttgtaa
     7741 tcgactatct tcagcttatg tcgagcagca gaaagattga cagccgtgta aatgaaattt
     7801 cggaaattac acgaaatctg aaaattcttg caaaggaaat gaatgttccc gtaatcaccc
     7861 tgtcacagct ttcccgtgct gcagaaaagc gtgatgacca cagaccgcag attgccgacc
     7921 tgagagattc gggttctatc gagcaggatg ccgatatcgt actgttcctt tatcgtgagg
     7981 gctactatga caaagagggc ggagaggatt ctgccgcacc gacagccgat atgaattcgg
     8041 gcgagtgtat cgtagcaaag aaccgtcacg gtgaaactaa tattgtaaaa ctgcattggc
     8101 agggcgagtt catgcgcttt acgggaacgg acaaccgaga tgcttaaccg tgttacagca
     8161 accgttgaaa aatacggctt gcttgaaaaa ggtgacagcg taatcgttgc actttcgggc
     8221 ggagctgatt ccacggcttt gttaagcgtt tttgtttctc tgaaagaaaa atacaatctt
     8281 tcgatttatg ccgctcatat caaccacaat atccgtggtg atgaggcaaa gcgtgatgaa
     8341 aatttctgca aaattctttg taagaaattt aacacggaac ttttcataaa aagcgttgat
     8401 gttcccacac ttgccaaaca gcagaaaatc agcgaagagc tttgcggaag aaatgtccgc
     8461 tacgctttct ttgaagagct ttcgcaaaaa ctttgtgcaa agattgcaac cgctcacacg
     8521 gcatccgaca atgccgaaac gctgatattc aatattgcaa ggggtgcgtc tttgacaggc
     8581 ttgtcggcaa ttcctccaaa acgggggaac ataatcagac cgctgattga actttcgaga
     8641 ggtgacattg aaagctattg cgaggaaaac aaccttgaat atgttaccga cagtacgaat
     8701 cttgccgatg attacacacg aaattacatt aggcataaca ttattccgtg tcttaaaaag
     8761 ctgaatcctt cttttgaacg gacggcttta ggcttgagtg aaaatgccgg agagtcggct
     8821 gatttcatta agaaatgtgc cgaaaaagcc gtgcatgact gcaaaagtga ttttggctac
     8881 gactgcaaaa agctgttgag ccttgattct gccgttctga aggaaatgtt gtttattctg
     8941 ttaaagaata attgcaacat ttctcccgaa agaaaaacaa ttctactatt gtgtgaaata
     9001 atcagaaacg gaggaagcgt tgaactgtca aaacaatgca cagccgtttc aaaacaggga
     9061 atactccgct ttgtatgtcc taaaaactcc cctgatttta atgaaattcc gttaaagaat
     9121 aatatgactt tttcttatga cggaaaaata tacacggtaa atgaaattac cgaaaaagta
     9181 acaaatcaaa ataacctcgt ttccaaaaag cggcttggcg aaaatcctgt ttttcgcaca
     9241 cgcagagcag gcgacaggtt tacatatccg gaccgcatgg tcacaaaacc gctccgcaag
     9301 gtttttaacg aacagaaaat cccgtccgaa atgagggaca ggctgttact gcttgcggta
     9361 tccaacaccg ttttgtggtg tgaaaagctt ggtgtttcat tgcagggaaa ggcagatgac
     9421 accgaaaaaa ccgaaaaatt actcataaat atagacaaag agggttaatt atgcataagg
     9481 atattgaaaa aattctgtat tcggaaaaag atattgaaaa tattgtgaac actattgcaa
     9541 aacagattga aaaagattat aatgacaaag attttataat ggtcggtctg ctcaagggca
     9601 gcgttgcctt tatggttgac cttatgagaa aggttaatct tgatttttca atcgacttta
     9661 tcgttgcttc aagctacggc agcggtactg taagctcagg cagagttaat attcagaagg
     9721 atatttccca gtctgttgag ggcaaggata ttctcattgt tgaggatatt atcgactcag
     9781 gcaatactct tgactttatc acaaaatatc tcaaagccaa gaaggcaaaa tcagtaaagc
     9841 tctgtacact ttttaacaag ccggacagac gggttgccga tatccatatt gactacgcag
     9901 gtgcggtaat tccggatgaa tttatcgtcg gctacggtct tgactatgac gaaaagtaca
     9961 gaaatcttcc ttatgtaggc gtattaaagc cgtcagttta ttcgtgattt taatgcgaaa
    10021 ataagccgca aattttcgtg gcattacata tttccacctc ggaaaaataa tactatacag
    10081 aggagtgaca gcattggaca ataaaaagaa agtgcgcaat ctgctgcttt atctcggaat
    10141 tcccattctg ataatcatta taacagcggc tgtgctgagc acaaataagt caacttcacc
    10201 gaaaacctcg gagcttgagc agtattttgt tcaggatatg gttgacagct ttaacattga
    10261 ctacggttca ggtcagattg aaattaccct taaagagggt atgagtccta tcaaatcaac
    10321 cgattccgac tctcaatctg caacaactgc tactcagccc gcaaccgaac agcagaaaac
    10381 ccagtcaaac agcaaaaaat caaagattgt tgtaaaagga cagcttgccg atattcagcg
    10441 tttccttgat gacatcaagc cgtactacaa tccggcgtca actgatgaaa ttccgcataa
    10501 ccttacccgt gcaacggata attccatact tatggaaatg attccgacac taatcattat
    10561 gattattctc atcgttgcct gggtgctcat tatgaagaaa atgggcggcg gaggtcttgg
    10621 cggcaaggaa atgagctttg gcaaagccaa gattaagaat acaaacgatg aaaagcgcaa
    10681 gacaacattt gatgatgttg caggtgccga tgaagaaaag gaagagcttg cagaggttgt
    10741 tgaattcctc aaggctcctg aaaagtataa caaactcggt gcaagaatac caaagggcgt
    10801 tctccttgta ggccctcccg gtacaggtaa aacattgctt gcaagagctg ttgcaggtga
    10861 ggcaggtgtg ccgttcttct caatttcagg ttcagacttt gttgagatgt ttgtcggtgt
    10921 cggtgcgtcc cgtgttcgtg acctctttga tcaggcaaag aaaaactcac cttgtatcat
    10981 ctttatcgat gagattgacg ccgtaggtcg ccagagaggc gcaggtcttg gcggcggcaa
    11041 cgatgagcgt gagcagactc ttaaccagtt gcttgttgaa atggacggtt tcggtgctaa
    11101 cgagggtgta atccttattg ccgcaactaa cagacccgat gttctcgatc cggctcttat
    11161 gcgtccgggc agatttgacc gtcaggttgt tgtaagctat cccgatgtaa acggccgtga
    11221 ggctatcctc agggttcatg caagaaagaa acctcttgca cccgatgtaa atctcaagac
    11281 tattgctaaa acaacagccg gatttacagg tgcagacctt gaaaatcttc ttaatgaggc
    11341 cgcacttctt gccgcaagaa aagataaaaa agccattact atggacgaaa tcaaggaggc
    11401 aacagtcaag gttgttgtcg gtgcagagaa aaagagcaaa gtaatgagtg agaaagagaa
    11461 aaagctcaca gcctatcatg aggcaggtca cgcaattctc tttgataagc tcgaaactca
    11521 ggatcctgtt cacgagattt caattattcc gacaggtatg gcaggcggtt acacaatgcc
    11581 gctcccgagt gaggacaagt catacaactc acgcagaggt atgtatgagg atatcgttgt
    11641 aagtcttggc ggtcgagttg ccgaagaact tattatggat gacatctcaa caggtgcgtc
    11701 aaacgatatt gaaaaggcta caaaaacagc ccgtgcaatg gttacaaagt acggtatgac
    11761 taaggaactc ggttgtgtat gctacggcac agacaacaat tccgtattcc tcggcagaga
    11821 tatgggcagc agaactcagg attattccga ggctaccgca gctaaaatcg atcagcttgt
    11881 tcttgatatg gtcaacaagg catatggcga cgctactaag attctcagcg ataatatgga
    11941 taagctccac gacattgcaa aatatcttat caagcatgag aagatgggca gcgaggactt
    12001 tactgccgta atgaacggca catataaaga acccgaagaa gttgagcagg aaactgccga
    12061 caaagaaccc tcagccgaaa caaaggttga cgagtccaca gaaggttaat atcaaataaa
    12121 catagcctct cgcaatgaga ggctatgcgt gtatatgaat caatatttaa acgaatgtat
    12181 gaagttgtgt caggtgtttg acacagtaaa gggtagatga aatggcacag gataaaatta
    12241 ttgtgagagg tgcaaaagag cataacctca aaaatgtaaa tgttgaaatt ccccgtaaca
    12301 agcttgtcgt aattacagga ctttcgggtt cgggcaaaag ctcgcttgca tttgacacca
    12361 tctatgccga gggacagaga agatatgttg aaagcctttc ttcctatgca agaatgtttc
    12421 tcggtcagat ggataagcct aatgttgaaa gcatagaggg actttcgcct gcaatatcaa
    12481 tcgatcagaa aactacttca aagaatcccc gttcaacggt cggtacggtt acggaaattt
    12541 acgattatct ccgccttttg tacgcaagaa taggtgttcc gcattgtacg gtttgcggaa
    12601 gggaaatccg tcagcagaca gttgaccaga ttgtcgataa gattatgacc tatgaaacgg
    12661 gtacgaaaat tcagattat
//
LOCUS       DAOMVE010000040        12582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1137_length_12582_cov_2.907560, whole genome shotgun sequence.
ACCESSION   DAOMVE010000040 DAOMVE010000000
VERSION     DAOMVE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_12582_cov_2.907560"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLS64_03575"
     CDS             <1..937
                     /locus_tag="PLS64_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HRL42943.1"
                     /translation="RWGSCIAWYLDKIGHNVLSCGLADAPEFIQLKETHKNDYLTFPK
                     SIEVSSNLEYAIERAEVIIISISSQYLRSYFADISKYNLDGKTIVLCMKGVEATTGKR
                     LSEVVGEFVDESKTPVAVWVGPGHPQDYVRGIPNCMVIDSNNHDIKEKLVNEFTSDLI
                     RFYLGTDLIGSEIGAAAKNVIGIAAGMLDGLNYTSLKGALMARGTREIARLIKALGGN
                     EMSAYGLCHLGDYEATLFSKWSHNRMYGESFAKGDRFTKLAEGVMTSKALYKLGQEYG
                     VDLPIVESVYKVLFDGTDIKEALGTLFMRSVKKEF"
     gene            957..2639
                     /locus_tag="PLS64_03580"
     CDS             957..2639
                     /locus_tag="PLS64_03580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRL42944.1"
                     /translation="MGNTKAKNNSNKPKQTGKTVTSVKKQNTVEYVIVNILCLFVFIA
                     FAYIAIMSFVQTSVFDSANYGSEVILYQTDNIALNILFTSLFTVFIFKMKKHCDFFAK
                     VNLKYMHIGLAAFVMIVGLVWIFSVTSVPAADSYNIYETASQAAKGNYSSFHNNSGFY
                     NSDFYSGYSYYNFYPFQLGFVFISEIFYRIFGTDSTMPIQVFNVMCTAAAYIGIVNIT
                     RLLFKKRSVEFITILLLAGCFQPVLFCTFVYGNIIGMCFAIWASYFLIKYFQTNKYLL
                     LIPCAVLLVISTLAKYNNLIYLVAFVVMLIIHTIKAKKWQSIAFALAICIAVVGTSNL
                     VIISYENRSGVKLSSGVSQAMYLDMGINDSYMAPGWYNGIALNDYKNAGLDAKAANAQ
                     AWTNIKSRMNYFGKNTNYMIDFFSKKIISQWNEPTYESIWVSKVKSHTNELNWIGNGM
                     YDGSIGQFFELYFNFYMQILFIAFAAGIYFLFINRKTNIETVLLPLVILGAFGYHLLF
                     EGKSQYVLTYIILMIPTASFAFECILNGKYTKIKEFVGKLKEIPDGKESEKA"
     gene            complement(2661..3527)
                     /locus_tag="PLS64_03585"
     CDS             complement(2661..3527)
                     /locus_tag="PLS64_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRL42945.1"
                     /translation="MVRIITDSAADFEHSESEKFGISTIPLAVYIDGKEYKDDFLHSK
                     ERFYRLAKLSKTLPKTSQPSPYVYECALKKARDNGESVVVITVSSALSGSYQSAVLAR
                     DLLGYEGCYVIDSKSASAGQKLLVNEALRLRDNGFTAKEIAEHLLRFRSKIMVYACID
                     DMKYLYGGGRHTNAAALLGSTGRIKPVISITGSGSVAFEAKTIGMRHGMNHMLMSLKK
                     LGIDQNYPLYIMHTNAKNQALYLSKLIISKGIPFVEGSIVSVGSVVGSHIGEGSTGIA
                     FVKKEQRKSGHD"
     gene            complement(3633..>4509)
                     /locus_tag="PLS64_03590"
     CDS             complement(3633..>4509)
                     /locus_tag="PLS64_03590"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRL42946.1"
                     /translation="KEYPRSDFRSVSDVDILFDRKQADSVKKVFSKLGYTFLNEDDNQ
                     YHFEKKPMMNIEMHATLVHENEESYPLLVNQLDRSTKRDGYSYSYEMSHEDFYIYMLV
                     HNSNHFRIGGMGARMVLDSYVFLKNHQSELDYDYLNVMLEKIGIAKYEKRVREIAFNW
                     FAKPQAELKFDDIEEYILLSGTLGRVDVGTMINSHKTITENNKSKFSYLVSSIFPPKS
                     EMQYKYPYVKKTPFLLPISWCHMWGKRLFVDRNINFKSGIKNRMSYTDEDVNLYKSLL
                     DEMGFDGIGINNFGN"
     assembly_gap    4510..4519
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4611..>4796)
                     /locus_tag="PLS64_03595"
     CDS             complement(4611..>4796)
                     /locus_tag="PLS64_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42947.1"
                     /translation="AVLSLDKKYVSPEVYEKFKQNLSVEMLIDGNLSYETEKILKAFD
                     ENKIKNIPLKGYFMKKE"
     assembly_gap    4797..4810
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4972..5454)
                     /locus_tag="PLS64_03600"
     CDS             complement(4972..5454)
                     /locus_tag="PLS64_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HRL42948.1"
                     /translation="MNKEVGLDDVMQIIAEKLETGGSVTFNPKGTSMLPMLRDGDDTV
                     VLSKPKGRLHLFDLPLYRRKDGSYVLHRVVNFGSDGSYTMCGDNQFAVEKGITDDDVI
                     GVVTAFYRKGKPYTVDSMKYRAYLEFMFYSKPLRKVIASTVSRSKNAFSKLKTKKGKK
                     "
     gene            complement(5447..5710)
                     /locus_tag="PLS64_03605"
     CDS             complement(5447..5710)
                     /locus_tag="PLS64_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRL42949.1"
                     /translation="MKIKEDFVLRKVADSYVVVPVNKLTLDFNGIINLNETGAFLFEK
                     LQNDCEKADLLKAMLDEYEVSEEKASADIDTFIQKLREADVLE"
     gene            complement(5859..7487)
                     /gene="groL"
                     /locus_tag="PLS64_03610"
     CDS             complement(5859..7487)
                     /gene="groL"
                     /locus_tag="PLS64_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354131.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRL42950.1"
                     /translation="MAKQIAYGEDARKALMKGIDQLADTVKITLGPKGRNVVLDKKFG
                     APLITNDGVTIAKEIELEDPFENMGAQLVKEVSIKTNDIAGDGTTTATLLAQALIREG
                     MKNVTAGANPMVLKKGIADAVNVAVEAVKKNSKQVSGTDDIARVATVSSQDEFIGKLI
                     AEAMEKVTTDGVITVEESKTAETYSEVVEGMMFDRGYIAPYMVTDTDKMVAELDNPLI
                     LITDKKISTTQEILPLLEQIVQMGKKLLIIAEDVEGEALTTLVLNKLRGTFTCVAVKA
                     PGFGDRRKEMLQDIATLTGGTVITSDLGLELKDTTVDQLGTARQVKIEKENTIIVDGS
                     GDKEAIKGRVAQIRQQIETTTSDFDREKLQERLAKLAGGVAVIKVGAATEVEMKEKKL
                     RIEDALAATKAAVEEGIVAGGGIACMNAIPAVAEFVDTLEGDAKTGAKIVLKALEEPL
                     RQIVANAGLEGSVICDNVKKANKVGYGFNALTEEYTDMVSAGIVDPTKVTRSALQNAS
                     SVAAMVLTTESLVTDIKEPAAPAAPAAPDMGGMY"
     gene            complement(7516..7800)
                     /locus_tag="PLS64_03615"
     CDS             complement(7516..7800)
                     /locus_tag="PLS64_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRL42951.1"
                     /translation="MTIKPLLDRVVLKVEEAEEKTKSGIILSSAAQEKPQFASVVAVG
                     PGGVVDGKEVKMYVSVGDKVIASKYAGTQVKIDGEEYTIVNQSDILATLE"
     gene            complement(8064..9680)
                     /locus_tag="PLS64_03620"
     CDS             complement(8064..9680)
                     /locus_tag="PLS64_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRL42952.1"
                     /translation="MQEIGDNKICPSCGFDTTEKQQAPFLPYGTILQNRYIVGAGIDT
                     NGESTRYISHDKQTGDIVIICEFLPIGLFSREEGTTEVRINYENRLVYNKLKDDFLNY
                     YRILSELRELSALMNVHNIFEENNTVYVVEENEDLIPFEEYVERSNGHLEWDIARPLF
                     MPVISALEALHKRGVGHYAIAPKNMYITASGKIKISGFASENERKRGTPLKSQLFSGA
                     AAPEQYDDNFPLDDITDIYGFCATLFYALTGHMPKSAVERRKDSRLLMSTTTVKRLPP
                     HVVSALANGLQVERENRITDFDELRSQLSVAHTAKAIQDEISRTASMNLTKSERTRKT
                     SNGMSHASIIIIAAAITVLVLGIAGVFWLMQNPLAGMFSNNTDASVSSTQSTEWTGAT
                     VPNYIGMTYEEAVKSVDSNSSITIYRTFNDEYSDDYAEGKIMSQTPAAGSKITQEDSV
                     VVSVCVSKGSQMRTLPKVEGEKLDTVASDIAAQGLLATAEYEYSDKVAEGRVIRYKEH
                     VEGDTLEYGSTVVLVVSKGKKQTSSQSSNR"
     gene            complement(9870..9945)
                     /locus_tag="PLS64_03625"
     tRNA            complement(9870..9945)
                     /locus_tag="PLS64_03625"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ala,seq:ggc)
     gene            complement(10011..10661)
                     /locus_tag="PLS64_03630"
     CDS             complement(10011..10661)
                     /locus_tag="PLS64_03630"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRL42953.1"
                     /translation="MADKGVIMRCVIIAGSPDTNSDFLSEVIKPDDYVICADRGCNFA
                     KQAGITPNLVVGDFDSEPDVLFPNCETVRLIPEKDDTDTMHSVDLALEKRFDEIAILG
                     ALGGRFDHSFANVAVLSYIHEHGSKGVLLSEKEKIEFLPVGHYEYKNFKGKTFSLFPF
                     GCPSVCVSYSGTKYPLEKYCVSSSVTLGVSNVFTSDMTTIDIYDGNAILIINLIDC"
     gene            complement(10643..11530)
                     /gene="rsgA"
                     /locus_tag="PLS64_03635"
     CDS             complement(10643..11530)
                     /gene="rsgA"
                     /locus_tag="PLS64_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRL42954.1"
                     /translation="MDTKSGILMKSIGGFYYVRADGEDYECKARGSFRKSGNSPVAGD
                     RVNISVPNDGFCAIEEILPRKNKLRRPALANLDILLLVCSTVEPLPNFTIIDKMTAAA
                     VNNNMEPVIVVTKNDLENGDKIADIYRHAGFEVFLCSEDDSSQTEELKSYLSGKVSAF
                     IGNSGVGKSTLLNKLFPSLSLETGQTSKKLGRGRHTTRVVELFELDGCFVADTPGFST
                     VDLQRYEMIDKSRLQYCFPEFEKYLGDCMFTSCSHTCEKGCRILEALSDGEIEETRHR
                     SYVQMYNEVKDIKSWQIKE"
     gene            complement(11541..>12582)
                     /locus_tag="PLS64_03640"
     CDS             complement(11541..>12582)
                     /locus_tag="PLS64_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HRL42955.1"
                     /translation="VCAIFFALAMFRGCSTSQAKDVEVPDYVGKSFVSIKENNPNDFK
                     FEVKSEYDESKEMGVILDQEPKGGSMKVKSGSTITVTVNGTDTEVSVPYVTNYSEKEA
                     SQALKEKNLIPEIVYVENTKTPKGYTIECFPKAGVKTTIGSTVYVYIASGERTKQVTL
                     PSVDGLMLSEAKQTLVDLGLTVETVQDDESKEPKDTVLGMSPLQYGKVDAGSVVTLTV
                     SSGLGDENTVNIFVDLPSDVEDSVDMTVIVDGAVDSRNSKTLVPKYNKTYTLELKGSG
                     TSTVVIQLDGQAYREYSVDFASAAVTTTASHDYVRATTAPPETEPPTQYYTDPYVAPD
                     TTVQPVTDDPEL"
BASE COUNT         3503 a         2749 c         2438 g         3868 t
ORIGIN      
        1 acgctggggc agctgcattg cctggtatct cgacaaaatc ggtcacaatg ttttaagctg
       61 cggacttgcc gacgcacccg aatttataca gcttaaagaa acacacaaaa acgactatct
      121 cacctttcca aagtcaattg aggtttcttc gaatcttgaa tatgcgattg aaagagccga
      181 ggttattata atttcgattt catcgcagta tctgcgttcg tattttgcag atatttcaaa
      241 gtacaatctt gacggcaaga caattgtcct ctgcatgaag ggtgttgagg caactacagg
      301 caaaaggctc agtgaggttg tcggtgaatt tgttgacgaa agcaagacac ccgttgcagt
      361 ttgggtaggt ccgggacatc cccaggatta tgtaagaggt attccgaact gtatggttat
      421 tgacagcaat aatcacgata ttaaggaaaa gcttgtaaat gaattcacaa gcgaccttat
      481 cagattttac cttggaacgg atcttatcgg cagtgaaatc ggtgctgccg caaagaatgt
      541 tatcggcatc gccgcaggta tgcttgacgg acttaactac acctcactaa aaggtgcgtt
      601 aatggcaaga ggcacacgag agattgcaag actcattaag gcactcggag gcaatgagat
      661 gagtgcttac ggtctttgtc atctcggcga ctatgaggct accttgtttt caaaatggag
      721 tcacaacaga atgtacggtg aaagctttgc aaagggagac agatttacaa agcttgccga
      781 gggtgttatg acatcaaaag cgttatacaa gctcggtcag gaatatggtg ttgaccttcc
      841 gattgttgag tctgtataca aggttttatt cgacggaaca gatatcaaag aggcactcgg
      901 aactctgttt atgcgttctg tcaagaaaga attttaatcg ggcaggtaca tataatatgg
      961 gtaatactaa agctaagaac aattcaaata aacctaaaca gactggaaaa acggttactt
     1021 ctgtcaaaaa gcagaataca gtcgagtatg ttattgtaaa cattctttgt ctgttcgtat
     1081 tcatagcatt tgcatacata gcaattatga gctttgtgca gacaagcgtg tttgattcgg
     1141 caaattacgg cagtgaagta attttgtatc agacggataa cattgcgctg aacatcctgt
     1201 tcacttccct gtttaccgtt tttatattca aaatgaaaaa gcattgtgac ttttttgcaa
     1261 aggtcaatct gaaatatatg catataggtc ttgcggcatt cgttatgatt gtgggacttg
     1321 tctggatatt ctcggtaacc tctgttcctg cggctgacag ctacaacatc tatgaaacag
     1381 cgtcgcaggc ggctaagggc aactattcgt cattccacaa taacagcggt ttttacaaca
     1441 gcgattttta cagcggatat tcttactaca acttctatcc gttccagctt ggctttgttt
     1501 tcatcagcga aattttttac agaattttcg gtacggacag tacaatgccg attcaggtat
     1561 tcaatgtaat gtgtacagcc gccgcttata taggtattgt aaatatcaca agacttcttt
     1621 tcaaaaagcg ttcggttgaa tttattacaa ttcttctgct tgccggctgt tttcagcccg
     1681 tacttttctg cacattcgtt tacggcaaca tcatcggaat gtgctttgca atctgggcat
     1741 catacttcct catcaagtat tttcagacaa acaagtacct gctcctcatt ccgtgtgccg
     1801 tacttctcgt tatttcaaca cttgcaaaat acaacaacct gatttatctt gttgcgtttg
     1861 ttgttatgct gattattcac acaattaagg ctaagaagtg gcagagcatt gcgtttgcac
     1921 ttgcaatttg cattgctgtt gtcggaacaa gcaaccttgt tatcataagt tatgagaacc
     1981 gttcgggagt gaagctttca agcggtgttt cacaggctat gtaccttgat atgggcatta
     2041 acgattcata tatggctccc ggttggtaca acggaattgc cctcaacgat tataaaaacg
     2101 caggtcttga cgcaaaagcc gcaaatgcac aggcttggac caatataaaa tcaagaatga
     2161 attactttgg taaaaacaca aactatatga ttgacttctt ctcgaagaaa attatcagcc
     2221 aatggaatga accgacatac gaaagcatat gggttagcaa ggtcaagtct catacaaacg
     2281 aacttaattg gatcggcaac ggaatgtatg acggcagtat cggacagttc tttgaactgt
     2341 acttcaactt ctatatgcag attcttttca ttgcgtttgc ggcaggaatt tacttcttgt
     2401 tcattaacag aaagacaaat attgaaaccg tacttctccc tcttgttatt ctgggagcgt
     2461 tcggatatca cctcttattt gaaggcaagt cgcagtatgt gctgacttac ataattctta
     2521 tgattcctac tgcgtctttt gcatttgagt gtattcttaa cggcaaatat acaaaaatca
     2581 aagaatttgt cggaaaactg aaagaaattc ccgacggtaa agaaagcgaa aaagcctgat
     2641 aaaatgattg aagcggacag ttaatcatgt ccgctttttc tttgctcttt tttgacaaat
     2701 gcaatacccg tactgccctc tcctatatga ctgccaacaa cagaacccac gctgacaata
     2761 ctgccctcaa caaaaggaat tccttttgat attatgagct ttgataaata caatgcctga
     2821 ttttttgcgt ttgtgtgcat tatatacagc ggataatttt ggtctatgcc aagctttttc
     2881 agcgacatca gcatatgatt cataccgtgg cgcattccga ttgttttcgc ctcaaaggct
     2941 acactgcccg aacccgtaat gctgattacg ggttttattc ttcctgttga gccgagtaat
     3001 gcggcggcat ttgtatgtct gccaccccca tagaggtact tcatatcgtc aatacaggca
     3061 tataccataa ttttacttct gaacctcaga agatgttcgg caatttcttt tgctgtaaac
     3121 ccgttgtcac gcaggcgaag tgcctcgttt acaagaagct tctgacctgc cgaagcagat
     3181 ttgctgtcta tcacataaca cccctcatat ccgagcaaat ctcttgcaag aactgcactc
     3241 tgatatgaac cgctcaatgc cgaagaaaca gttatcacca caaccgattc gccgttatct
     3301 ctcgcctttt tcaaagcaca ttcgtagaca tacggcgagg gttgagaggt tttcggcaaa
     3361 gttttcgaca gctttgcaag cctgtaaaag cgttcctttg aatgtaaaaa atcatcctta
     3421 tattcttttc cgtcaatata cacagctaac ggaattgtag aaattccgaa tttttcactt
     3481 tctgaatgtt caaaatcggc ggctgaatcg gtaataattc tgaccattta ggtatcacct
     3541 caaaaataat atcttgaaat attattttca aatttacctt tattatgcaa aaatcccgac
     3601 agcaaaaact gtcgggaggt tgatttgcat attcaatttc cgaaattatt tattccaata
     3661 ccgtcaaagc ccatttcatc caaaagggac ttgtacaaat ttacatcttc atccgtgtat
     3721 gacatacggt ttttgatgcc cgatttaaaa ttgatatttc tgtctacaaa aagccttttt
     3781 ccccacatat gacaccatga aatcggtaaa aggaacggtg ttttttttac atatggatac
     3841 ttatactgca tttcggattt tggtggaaat attgaggaca caagatatga gaatttagat
     3901 ttgttattct cggtaattgt cttgtgggaa tttatcatcg tgccgacatc taccctgccg
     3961 agtgtaccgc tgagcagaat atattcttcg atatcatcaa atttaagttc agcctgtggc
     4021 tttgcaaacc agttgaacgc aatctcacga acacgctttt catactttgc aataccgatt
     4081 ttttcaagca tcacattaag atagtcataa tcaagctcag attgatgatt tttcaagaaa
     4141 acatagctgt caagcaccat tcttgctccc attccgccga ttctgaaatg attcgagtta
     4201 tgaacaagca tatagatata aaagtcctca tggctcattt catatgaata gctgtagccg
     4261 tcacgctttg ttgaacggtc aagctgatta acaagcaacg gataagattc ctcgttttca
     4321 tggacaagag ttgcatgcat ttcaatgttc atcatcggct ttttttcaaa atgatactgg
     4381 ttgtcatctt cattcagaaa agtgtatccg agtttggaaa atactttttt gaccgaatca
     4441 gcctgttttc tgtcaaaaag gatatctaca tcagacacgg aacgaaagtc ggaacgggga
     4501 tattcctttn nnnnnnnnna tgaataaatg tcttgttttt tcaccatatt ctgttctcat
     4561 ccaagaattg gatgtaataa aagttaaaac cccatttttt ttcaaaacat ctattccttt
     4621 ttcataaaat agccctttaa gggaatattc ttgattttgt tttcatcaaa agccttgaga
     4681 attttctcgg tttcatatga gagatttccg tcaatcagca tttcaaccga caaattctgc
     4741 ttgaattttt catatacttc gggcgaaaca tactttttgt caagcgacaa aaccgcnnnn
     4801 nnnnnnnnnn ggcaacaagc gattcaacac tgcaatagtt tgcaagcatt tttatcgact
     4861 gccattttat cccctcataa ggaatcggag gtgctgttcc gtttacagac gatgaaacaa
     4921 gctcaagcag atacttaaat tcaatttttt cgttatcgtt aagatgtttc ctcatttttt
     4981 cccttttttc gttttaagct ttgagaatgc atttttactt cttgacacag ttgatgcaat
     5041 aacctttctt aacggttttg aatagaacat aaattcaagg tatgctctgt acttcattga
     5101 gtcaaccgtg tagggcttgc cctttctgta aaaggctgtt accactccga ttacatcatc
     5161 atcggtaata cctttttcaa cggcaaactg attgtcaccg cacatagtat aactgccgtc
     5221 acttccgaaa ttgacgacac ggtgcaatac atacgagccg tcttttcgtc tgtataaagg
     5281 caagtcaaaa agatgaagtc tgcccttcgg ttttgataaa acaaccgtat cgtcaccgtc
     5341 tcggagcatt gggagcatac ttgttccctt cggattgaaa gtaaccgatc ctccggtctc
     5401 aagcttttcg gctataatct gcattacatc atcaagacct acttctttat tcaaggacat
     5461 cagcctctct tagcttttga ataaatgtat caatatcggc actcgccttt tcttcgctca
     5521 cttcatactc atcaagcatt gctttgagca aatcagcctt ttcgcaatcg ttttggagct
     5581 tttcaaaaag gaatgcaccc gtttcattga ggttgataat tccgttaaaa tcaagcgtca
     5641 gcttatttac gggaactaca acatacgaat ctgcaacctt tcgcagaaca aaatcttctt
     5701 ttattttcat aaaatcacct gttttccgcg tgaatttttc atccatatat tataatatac
     5761 ctttcgacaa aaagcaagaa aaaggatttg agcataaaat taccgcagac cttttgagtc
     5821 tgcggtaaaa attatctata atattacagc ttacgggatt agtacattcc gcccatatca
     5881 ggtgctgcgg gagctgccgg agctgcaggc tctttgatgt ctgtaacaag tgattctgtt
     5941 gtaagaacca ttgctgcaac agagcttgcg ttctggagag ctgaacgagt aacctttgtt
     6001 gggtcaacga tacctgccga aaccatatct gtgtattcct ctgtaagagc gttgaagcca
     6061 tagccaacct tgttagcctt cttaacatta tcgcagatta cgctgccctc aagacctgcg
     6121 ttagcaacaa tctgacggag cggttcttca agtgctttaa gaacaatctt agcacctgtc
     6181 ttagcgtcac cctcgagagt gtcaacaaac tctgcaacag ccggaatagc gttcatgcaa
     6241 gcgataccgc cgcctgctac gataccctct tcaacagcag cctttgtagc ggcaagagca
     6301 tcctcaatac ggagcttctt ttctttcatt tcaacttctg ttgcagcacc aaccttgata
     6361 actgctacgc cgcctgcgag ctttgcaaga cgctcctgaa gtttttcacg gtcaaagtca
     6421 gatgttgttg tttcaatctg ctgtctgatc tgagcaactc tgcccttgat agcttccttg
     6481 tcacctgagc cgtcaacgat gattgtgttt tccttttcaa tcttaacctg acgggcagta
     6541 ccgagctgat caactgttgt atccttgagt tcaagaccga ggtcggatgt gataaccgta
     6601 ccgcctgtga gtgttgcgat atcctgaagc atttcttttc ttctgtcacc aaagcccgga
     6661 gcctttacgg caacacaagt gaatgtacct ctgagtttgt taagtacgag agttgtaaga
     6721 gcctcgcctt caacatcttc agcgataata aggagcttct tacccatctg aacaatctgc
     6781 tcaagaagtg ggagaatttc ctgtgtagta gaaatcttct tatctgtgat aaggataagc
     6841 ggattgtcaa gctctgcaac catcttatct gtatctgtta ccatataagg agcgatgtaa
     6901 cctctgtcaa acatcatacc ttcaacaacc tcgctgtatg tttcggctgt ctttgactcc
     6961 tcaactgtga taacaccgtc tgttgtaacc ttttccatag cctcggcaat aagcttgccg
     7021 atgaattcat cctgtgatga aactgttgca actcttgcga tatcgtctgt accggatacc
     7081 tgcttgctgt tcttctttac agcctcaact gcaacattta cagcgtctgc aatacccttt
     7141 ttaagtacca ttgggttagc gcctgcagtt acattcttca taccctctct gatgagagcc
     7201 tgagcaagaa gtgtggcagt tgttgtaccg tcgcctgcga tatcgttagt cttgatagaa
     7261 acttccttta caagctgagc acccatattt tcaaaaggat cttcaagttc aatttcctta
     7321 gcaattgtta caccgtcatt tgtgatgagc ggagcaccga acttcttatc aagaacaaca
     7381 tttctgccct taggtccgag tgtgatttta acggtatcag cgagctggtc gatacctttc
     7441 attaaagctt ttcttgcatc ttcgccgtat gcgatttgtt tagccattgt aaattacctc
     7501 cgtatattta gttaattatt caagagttgc gagtatatcc gactggttta cgattgtgta
     7561 ttcttcgccg tcaatcttta cctgtgtgcc tgcatattta cttgcgataa ccttatcgcc
     7621 tacgctgaca tacatcttta cttccttgcc gtcaacaaca ccgccggggc caacagcaac
     7681 aactgatgcg aactgtggct tttcctgagc agcagatgaa agaatgatac cgctctttgt
     7741 tttctcttca gcctcttcaa cttttaaaac gactctgtcg agtaatggtt tgattgtcat
     7801 aaatacagcc tcctgtaaaa attttaaatt gcttttaatt aattagcact cttgttccct
     7861 gagtgctaat tatattttat accattttca agaaaagtca agagaaattt gactattttt
     7921 gacctttatt ttgtgacagt tttctgaaat tttttccaat ctcaatttta tcgagtattt
     7981 taagataatc agtaactata actgttttat ttcaataaaa aagacggact gcaaaaagtc
     8041 cgtcttaaac attgttatta attttatctg tttgatgatt gagaacttgt ctgtttcttg
     8101 cccttgctga caacaagaac taccgttgaa ccgtattcga gagtatcacc ctcaacatgt
     8161 tctttgtagc ggattaccct tccctcagca accttgtcac tgtactcata ttctgctgtt
     8221 gcaagaagtc cctgagcggc aatgtcggat gcaacggtat caagtttttc accctcaacc
     8281 ttcggcagag tcctcatctg tgatcccttg cttacgcaaa cactcactac tacggaatct
     8341 tcctgagtga tttttgaacc tgcggcagga gtctgtgaca taatcttacc ttctgcataa
     8401 tcatcgctgt actcatcgtt aaaggttctg tagattgtga tactgctgtt gctgtcaaca
     8461 ctcttaacag cctcttcata tgtcattccg atatagttag gaacagtagc acctgtccac
     8521 tctgtgcttt gagttgagct tacactcgca tcagtattat ttgagaacat tccggcaagc
     8581 ggattctgca taagccagaa cacaccggca ataccgagaa caagaaccgt gatagcagcc
     8641 gcaataataa taattgaagc gtgtgacata ccgtttgaag tcttgcgtgt acgctctgac
     8701 tttgtgaggt tcatgcttgc tgttcttgaa atttcgtcct gaattgcctt tgctgtatga
     8761 gcaacggaaa gctgtgaacg aagctcgtca aaatctgtaa tacggttttc acgctcaacc
     8821 tgcaaaccgt tagcaagagc cgaaacaaca tgcggaggaa gtctctttac tgttgttgta
     8881 ctcataagaa gtcggctgtc cttacggcgt tctaccgcac tctttggcat atgacctgtg
     8941 agggcataga aaagggttgc acaaaatccg tagatatccg taatatcgtc aagaggaaaa
     9001 ttatcgtcat actgttcggg agccgcagcg cctgagaaaa gctgagattt gagaggcgtg
     9061 cctcttttac gctcattttc cgacgcaaag cctgaaattt taattttacc cgatgctgtg
     9121 atatacatat tcttaggtgc gatagcatag tgaccgacac ctctcttgtg aagagcctca
     9181 agagcagaaa ttacaggcat aaaaagcgga cgggcaatat cccattcaag atgaccgtta
     9241 ctgcgttcaa catactcctc aaaaggaata agatcctcgt tttcctcaac aacataaact
     9301 gtgttatttt cttcaaaaat gttgtgaaca ttcataagag cggaaagctc acggagttct
     9361 gaaagaatcc tgtaatagtt aagaaaatcg tccttgagtt tattatatac aaggcggttt
     9421 tcataattta ttctgacctc ggtagttcct tcttcacgag aaaaaagtcc gataggaaga
     9481 aattcgcaga tgattacaat gtcacctgtc tgcttgtcgt ggcttatata gcgtgtgctt
     9541 tcgccgttgg tatcaattcc cgcacctacg atatagcggt tctgaagaat tgttccgtac
     9601 ggtaaaaaag gagcttgctg tttttcagtt gtatcaaatc cgcaggacgg acaaatcttg
     9661 ttgtcaccta tttcctgcat gcatcccata cacaatgaca tattattact caactccaaa
     9721 taagtatcaa tttcaaattt tgtctgaaaa gaattatacc ataaaaacat atttcttttc
     9781 aagacttaaa ggtgttaata tagttacaat tttgttattg caaagcctta taaagcaaat
     9841 tctttgcaaa tattgttgta taaaaaatgt ggtggagata acggggctcg aacccgtgac
     9901 cttctgactg ccagtcagac actctcccag ctgagctata cccccatata atgttgacat
     9961 tgcttttgac atatacaaga tacatttgca aacaatgtca agctgtaatt ttaacagtct
    10021 atcaaattaa ttataagtat tgcatttccg tcatatatgt caattgttgt catatcggaa
    10081 gtgaatacat ttgaaacacc cagagtaacg ctgctcgaaa cacagtattt ttcaagagga
    10141 tactttgttc ccgaatagct tacgcagaca ctcggacatc cgaaagggaa aagcgaaaat
    10201 gttttgcctt taaagttttt atattcataa tgacctacgg gaagaaattc tattttctct
    10261 ttttcagaaa ggagaacgcc cttacttccg tgttcgtgaa tataagagag aaccgccaca
    10321 tttgcaaagc tgtggtcaaa gcgaccgccc aatgcaccga gaattgctat ttcgtcaaac
    10381 cttttttcaa gtgcaaggtc aacagagtgc attgtgtcgg tatcatcctt ttcgggaata
    10441 agtcggaccg tttcgcagtt tggaaacagt acatcaggct cggaatcaaa atctcctaca
    10501 acaaggttcg gagtaatgcc tgcctgtttt gcaaaattgc agcctctgtc ggcacatatc
    10561 acatagtcat caggttttat aacctcggac aaaaaatccg aatttgtgtc aggcgagcct
    10621 gcaataatca cacaacgcat aattactcct ttatctgcca tgatttaatg tccttgacct
    10681 cgttatacat ctgaacataa ctgcggtgac gggtttcttc aatttcaccg tcagagagcg
    10741 cctcaagaat cctgcagcct ttttcacagg tatgcgaaca tgaagtaaac atacagtccc
    10801 ctaaatattt ttcaaattca ggaaagcaat attgcagccg gcttttgtca atcatttcgt
    10861 aacgctgaag atcaacggtt gaaaatccgg gtgtgtctgc cacaaaacaa ccgtcaagtt
    10921 caaacagttc aacaactctt gttgtatgtc ttcctctgcc gagttttttg cttgtctgcc
    10981 ctgtttcaag agaaagcgat ggaaaaagct tattgagcaa tgtggacttg ccgacgccgc
    11041 tgttaccgat aaaggctgaa accttgccgg acagatagct tttgagttct tcggtttgtg
    11101 aagaatcatc ttccgaacag agaaaaacct caaaacctgc gtgtctgtag atatcggcaa
    11161 tcttatctcc gttttccaga tcattttttg taacgacaat tacgggttcc atattattgt
    11221 ttaccgccgc cgcagtcatt ttgtcaataa tcgtaaaatt cggcaacggt tcaacagttg
    11281 aacacacgag aagaagaata tcaagatttg caagagcagg tctgcggagc ttgtttttgc
    11341 gtggaagaat ttcttctatc gcacaaaaac cgtcattcgg aacagatata tttaccctgt
    11401 cccccgctac gggagagttt ccgcttttgc gaaagctccc ccttgcctta cattcgtaat
    11461 cctcgccatc ggcacggaca tagtaaaatc cgccgattga tttcattaaa attccgcttt
    11521 tagtatccat acttcaccga ttaaagttca ggatcatccg taaccggctg aacggtcgtg
    11581 tcaggcgcta catacggatc tgtgtaatac tgcgtcggcg gttcggtttc gggaggagcg
    11641 gttgtcgcac ggacataatc atgtgacgcc gtagtggtta ccgctgccga tgcaaagtca
    11701 accgaatact ctctgtacgc ctgaccgtca agctgaatta ctacagttga agtgcctgag
    11761 cccttgagct caagagtata ggtcttgtta tatttcggaa caagtgtctt tgaattacgg
    11821 gaatcaaccg caccgtcaac aattacagtc atatcgactg aatcttcaac atccgacgga
    11881 aggtcaacga agatattaac cgtgttttcg tcaccgagtc ccgagcttac agtcagggtt
    11941 acaacagagc ctgcgtcaac cttgccgtac tggagaggcg acataccgag gactgtatcc
    12001 tttggttctt tgctttcatc atcctgaact gtttcaacgg taagtccgag atcaacaaga
    12061 gtctgctttg cttcggaaag cataagtccg tcaaccgacg ggagtgtaac ctgtttggtt
    12121 ctctcaccgc ttgcaatata tacatatacc gttgaaccga ttgtagtttt tacacctgct
    12181 ttcgggaagc actcaattgt gtagcccttt ggtgttttag tgttttctac atagacaatt
    12241 tcaggaataa gattcttttc cttgagagcc tgagaagcct ctttttcgct gtaattggtt
    12301 acataaggaa cagaaacctc tgtgtccgtt ccgtttacgg ttaccgtgat tgttgagcct
    12361 gacttaacct tcattgaacc gcccttaggc tcttgatcaa gaattacgcc catttccttt
    12421 gactcatcat attcactctt aacctcaaac ttgaaatcat tcgggttatt ttcttttatt
    12481 gaaacaaagg atttgccgac ataatcggga acttcaacat ccttagcctg tgaggttgag
    12541 cagcctctga acattgcaag tgcaaagaaa attgcacaca ct
//
LOCUS       DAOMVE010000041        12403 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1161_length_12403_cov_3.721250, whole genome shotgun sequence.
ACCESSION   DAOMVE010000041 DAOMVE010000000
VERSION     DAOMVE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12403
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1161_length_12403_cov_3.721250"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..620
                     /gene="rplF"
                     /locus_tag="PLS64_03645"
     CDS             78..620
                     /gene="rplF"
                     /locus_tag="PLS64_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRL42956.1"
                     /translation="MSRIGRKPINIPAGVTASIADGVITVKGPKGSLDFKYNPAMTVE
                     VKGDVIEVTRPDDTKENRSLHGLTRTLIHNMVIGVTEGYKKTLEVNGVGYRVQKQGNK
                     CVMNLGYSHQVIVEDTEDIKIEVPDPNKIIISGIDKQKVGQFAAEVREKRPPEPYKGK
                     GIKYADEVIRRKEGKAGKGK"
     gene            639..998
                     /gene="rplR"
                     /locus_tag="PLS64_03650"
     CDS             639..998
                     /gene="rplR"
                     /locus_tag="PLS64_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRL42957.1"
                     /translation="MVSRPDTKKARAKRHKRVRSKISGTASCPRLCVFRSTSNIYAQI
                     IDDVAGVTLAQASSLDKEITGNGGNKEAAKAVGKLVAERAKEKNIVDVVFDRGGNIYH
                     GRVKELAEGAREGGLNF"
     gene            1016..1513
                     /gene="rpsE"
                     /locus_tag="PLS64_03655"
     CDS             1016..1513
                     /gene="rpsE"
                     /locus_tag="PLS64_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRL42958.1"
                     /translation="MAKTVEVTGELKERVVTINRVSKTVKGGRIFKFAALVVVGDGNG
                     TVGFGIGKAGEVPDAIRKGIEDAKKNLMKVSLRGTTIPHEVIGEFGAGRVLMKPAAPG
                     TGVIAGGPVRAVVEAVGIKDIRTKALRSNNPCNVVRATLAGLGALRNAEEVAAIRGKS
                     VKEIL"
     gene            1531..1701
                     /gene="rpmD"
                     /locus_tag="PLS64_03660"
     CDS             1531..1701
                     /gene="rpmD"
                     /locus_tag="PLS64_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRL42959.1"
                     /translation="MTIKLVKSLIGSKKNQIATAHALGLKKIGDVTTQPDNAQTKGKV
                     AKIIHLVEVSEA"
     gene            1720..2163
                     /gene="rplO"
                     /locus_tag="PLS64_03665"
     CDS             1720..2163
                     /gene="rplO"
                     /locus_tag="PLS64_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRL42960.1"
                     /translation="MKLHELSPVEGSKKDSKRIGRGHGSGWGKTSGKGHKGQKARSGG
                     SIRPGFEGGQMPLQRRIPKRGFNNIFAKAVAAVNLSTLNRKFEDGATVDIEALVNAGV
                     VKNGFDAVKILGNGKLDKKLTVKVSAFSESAKAAIEAAGGKAEVI"
     gene            2164..3474
                     /gene="secY"
                     /locus_tag="PLS64_03670"
     CDS             2164..3474
                     /gene="secY"
                     /locus_tag="PLS64_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRL42961.1"
                     /translation="MFKTIKNAWSLPDLRKKILFTLLIIVVFRIGSVIQVPFLDTAAL
                     RSVMNPDDWSNTMLSYMNTLSGGAFSNATLFAMGITPYINSSIIIQLLCVAIPPLERL
                     AREGEAGRRKISAITRYVTVGLGIIQGTAYYFYLLNSKVTLYNSGFELWFSAIVIILV
                     FTAGTAVMMWLGEQINSHGIGNGISILLFAGIVAQFPQIINTLGQYWNLAVNGSTQFF
                     FLVPLWVVLFVAVVWIITFMQDSERRIPIQYAKRVVGRKMYGGQSSHLPIKVALGGVL
                     PIIFASSILSIPSTINLFLKIPAGEGFWGAFFAAFSTTGWLYMVLYFLFIIMFAYFYT
                     SIQYNPVEMANNLKANNGTVPGIRPGAPTADYIRKILSRITLIGAMFLAVIAMIPLIY
                     GAASGMGQMSIGGTSIIIVVGVALETVKQLESQMMMRHYKGFLD"
     gene            3498..4130
                     /locus_tag="PLS64_03675"
     CDS             3498..4130
                     /locus_tag="PLS64_03675"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRL42962.1"
                     /translation="MNIIMLGAPGAGKGTQAAVLCEHFGIPTISTGNMIREALKNGTE
                     MGLKAKSFMDEGKLVPDEVVIGIVKERLSADDCKKGFILDGFPRTIPQAEALDNMGVE
                     IQHVINIDISDEKIINRMSGRRVCENCGRPFHIVTLKPKKEGVCDDCGGTLVQRKDDH
                     PDTVLARLETYHKETEPLVSYYEKQGKLVTVKGQDDVEATTKAILEGIEA"
     gene            4134..4886
                     /gene="map"
                     /locus_tag="PLS64_03680"
     CDS             4134..4886
                     /gene="map"
                     /locus_tag="PLS64_03680"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRL42963.1"
                     /translation="MVVVKTARELSKMKDACRISAEALRVAGEAVKPGVTTYEIDNIV
                     RSYIEKQGAVPSFLGYGGFPASACISVNNVVIHGIPSKELVLKEGDIVSVDVGAFYGG
                     FHGDNAFTFPCGKISADAQALLDATKESLYEGIKQALAFNRVGDIGNAVQKYVEARSY
                     SVVRDFVGHGVGAKLHEDPSVPNYGTAHRGVRLIPGMTIAIEPMVNQGRCNVRVLDDK
                     WTTVTADGKLSAHFEHTVAITPDGPKIMTLCD"
     gene            4897..5142
                     /locus_tag="PLS64_03685"
     CDS             4897..5142
                     /locus_tag="PLS64_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42964.1"
                     /translation="MNTVKGSIVKAVAGREKNGFFVVLDCDSVFAYIADGKRRKVEKP
                     KKKKLIHISPTGTVIEGSIKTNPQIRKILNNFRKSEG"
     gene            5147..5365
                     /gene="infA"
                     /locus_tag="PLS64_03690"
     CDS             5147..5365
                     /gene="infA"
                     /locus_tag="PLS64_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRL42965.1"
                     /translation="MSKQDVIEIEGIVKEALPNAQFKVELPNGHVILAVISGKLRMNF
                     IRILPGDKVKVEMSPYDLTRGRITWRTK"
     gene            5407..5520
                     /gene="rpmJ"
                     /locus_tag="PLS64_03695"
     CDS             5407..5520
                     /gene="rpmJ"
                     /locus_tag="PLS64_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRL42966.1"
                     /translation="MKVRPSVKKMCDKCKVIKRKGKILVICENPKHKQRQG"
     gene            5575..5943
                     /gene="rpsM"
                     /locus_tag="PLS64_03700"
     CDS             5575..5943
                     /gene="rpsM"
                     /locus_tag="PLS64_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRL42967.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIYGIGRKTADDIIAATGVNPDTR
                     VRDLTEEDIAKLREYIEHHCRVEGDLRRDIAYDIKRLIDIGCYRGVRHRKGLPVRGQR
                     SKTNARTRKGPRKTMANKKK"
     gene            5959..6360
                     /gene="rpsK"
                     /locus_tag="PLS64_03705"
     CDS             5959..6360
                     /gene="rpsK"
                     /locus_tag="PLS64_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRL42968.1"
                     /translation="MAAPKGGKKVIRRRRERKHIEKGAAHIQSTFNNTIVTISDTQGN
                     AVSWASAGELGFRGSKKSTPFAAQSAAETAAKAAMEHGMKYVEVYVKGPGQGREAAIR
                     ALQTAGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            6387..6989
                     /gene="rpsD"
                     /locus_tag="PLS64_03710"
     CDS             6387..6989
                     /gene="rpsD"
                     /locus_tag="PLS64_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015564648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRL42969.1"
                     /translation="MAKNMQPIAKRCRALGISPAVMGYSKKTSNRNPGGKMRRKKSEY
                     SMQLTEKQKVKFIYGIMEKQFRAYYEKAEKAEGKTGAVLLTLIERRLDNVIFRLGLAA
                     TRREARQLVSHSHFTVNGKKVNIPSYLVKVGDVIEVKEKSRSTARFKAIAEGETSSIV
                     TPKWLEKNENLLGGKVVAAPQREDIDFPVEEHLIVELYSK"
     gene            7064..8017
                     /locus_tag="PLS64_03715"
     CDS             7064..8017
                     /locus_tag="PLS64_03715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRL42970.1"
                     /translation="MIEIEKPRIETEELTEDGKYGKFVVEPLERGFGNTLGNSLRRVL
                     LSSLEGCAVTSIKIDGVLHEFSTIPGVKEDVTEIVLNMKSLVAKLHETSPKVIEIAAE
                     GPCEVKAADIKCDDEVEILNPELHIATLGEGAKLNMEITVDKGRGYIPSERNKQLSGN
                     NVIGVLPIDSIYTPVLKVNYTVDNTRVGQITDYDKLTLDVWTNGVINAQEAVSLAAKV
                     LTEHLNLFVNLSDKASSAEVMVEKDDKGKEKILEMTIEDLDLSVRSFNCLKRAGINTV
                     EDLINKSEEDMMKVRNLGRKSLEEVVQKLNSLGFSLQKEDE"
     gene            8090..8431
                     /gene="rplQ"
                     /locus_tag="PLS64_03720"
     CDS             8090..8431
                     /gene="rplQ"
                     /locus_tag="PLS64_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRL42971.1"
                     /translation="MPGTRKLGRTTAQRTAMLRAMVTFLLENGKIETTLTRAKEVQAM
                     AEKMITTAKEDTLHNRRLVLAFVTKEDVTSKLFKEIAPKYSDRNGGYTRVTKIGPRRG
                     DAAEMAIIELV"
     gene            complement(8784..9734)
                     /locus_tag="PLS64_03725"
     CDS             complement(8784..9734)
                     /locus_tag="PLS64_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRL42972.1"
                     /translation="MRNYGKKKNYCLIAALASLVAFSTMAASVMSGCGENSDEQKTTK
                     IVHETRIDTQIETKIETYTEVVTDAQGNTEYVKETKAVPVNNNAGGNSKSGDSGNNSE
                     NSGGSSSGKNSNSQQSSVIEKSETSKTTNNSSSKTDSGKTNNNSNKNNNNSNKNNNSN
                     KNNNSSSSKSDSSKSDDSNSKSLKIGGKTFNVGDTIVCTYNLTTPAVLENYQATIKYD
                     SSKLTCKSAKMSVPAKYGAMLNIKIAGEISLSGVNIGDGYDYTNGGAFMTVVYEVKGG
                     GSTSPSYHWEVACRASDSKNLIDKKGKPASGFKLSSSYKD"
     gene            9982..11970
                     /locus_tag="PLS64_03730"
     CDS             9982..11970
                     /locus_tag="PLS64_03730"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRL42973.1"
                     /translation="MRTTKKALSIVLAGLMTVGGMSVFSVSAAQTSTPTLSFKTQNAL
                     YAHAVSGSDDSDAWVAWQCKHNEDMEELNTNRKYFFLPSSVSSTSVELYNAYSKSVTV
                     NNVTIPSGESREVSYTIDKAGNVTADGKTYSLTFLKSSAESAIYVNNSNADGNGKELI
                     SYLNEDKSNYSSATGAIVDRNGKIDNTSIKKIKGRGNSTWGKAKKPYNITYSDKVSIG
                     GMSKGKKFSLLANYQDDSLTRNRFLYDLADAVGTPYASDSRYVDFYSDGYYWGSYQMT
                     EKIEVGKSALVNDIDDTAYLDADGNVNKDFPFLCEVDSGAVDGEDYYVKCDDGIKVTI
                     KAPELSEGDKGYNEVKNYVREKYNAFYRAAKKADSDLSQYADVDSCAKLWLINELGKN
                     WDSGVSSVYFVYKQDSDGNYKFFGSPVWDYDNALGNAAGSAWDLQNFGVKDYTQYSGW
                     WCRFKDRQKRSQNSSNIINNISRNTQVNKAAVNIWFEQFVPAINYFAGKTTSYKGSDE
                     FYTKTQYFDLLKDSGAMNYKSGWHIRTSSWICDHTSMNKADFDMTTGTYTVSNTKTSY
                     NQGSFTDMYNYAADWMTSRAAWISSKWFSEYTPSAKIGDVDGDGEVTVMDATLVQKYI
                     VSLETLTDSQLNVADVNGDGEISVIDATQIQKIVVNLV"
     gene            12178..>12403
                     /locus_tag="PLS64_03735"
     CDS             12178..>12403
                     /locus_tag="PLS64_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRL42974.1"
                     /translation="MRKTKIVCTIGPACDSKEMMRKMIDAGMNVARINMSHGVYDRLE
                     VTIKNLKEAIAESGQNVAILLDTKGPEVRVG"
BASE COUNT         3669 a         2467 c         2872 g         3395 t
ORIGIN      
        1 aatgacagat cgtcaggctc gtgctgaaca tattggcggc gaagttcttg cttacatttg
       61 gtaaggaggt acgataaatg tctcgaattg gaagaaaacc tataaatatt cccgccggag
      121 ttacagcttc aatcgctgac ggcgttatca ctgttaaggg accgaagggc tcacttgatt
      181 tcaagtacaa cccggctatg acagtagaag ttaagggcga tgtaatcgaa gttacccgcc
      241 ctgacgacac aaaagaaaac cgctcacttc acggccttac aagaacactt atccacaata
      301 tggttatcgg tgttacagag ggttacaaga agactcttga ggttaacggc gttggttacc
      361 gtgttcagaa gcagggtaac aagtgcgtta tgaaccttgg ttattcacac caggttatcg
      421 ttgaggatac agaggacatc aagattgaag ttcccgatcc taacaaaatc atcatctccg
      481 gcattgataa gcaaaaggtt ggtcagtttg ctgccgaagt aagagaaaag cgtcctccgg
      541 agccatataa gggcaagggt attaagtacg ctgatgaagt tatccgccgc aaggaaggca
      601 aggccggtaa gggcaaataa ttaaggagtg aataattaat ggtaagcaga ccagatacaa
      661 aaaaggcacg cgcaaaacgc cataagagag tccgcagcaa gatcagcggt acagcttctt
      721 gtcctcgtct ttgcgtattc cgctccacaa gcaacatcta tgctcagatt attgacgatg
      781 tagccggtgt tacacttgct caggcttcaa gccttgataa ggaaatcaca ggcaatggcg
      841 gaaacaaaga ggcagctaag gctgttggca agctcgttgc tgagcgtgca aaagaaaaga
      901 atatcgttga tgttgtattc gacagaggcg gtaacatata tcacggccgt gttaaggaat
      961 tggccgaagg cgcccgtgag ggcggcctca atttctaagg attggaggaa taactttggc
     1021 taaaacagta gaagtaacag gcgaattaaa agaaagagta gttactatta accgtgtttc
     1081 taaaacagtt aaaggcggtc gtattttcaa gtttgcagct ctcgtagttg taggcgacgg
     1141 taacggaaca gttggtttcg gtatcggtaa agcaggcgaa gtacccgacg ctatccgcaa
     1201 gggtattgag gatgctaaga agaacctcat gaaggtttca ctcagaggta caacaatccc
     1261 tcacgaagta atcggtgagt tcggtgcagg cagagttctt atgaagcccg cagctcccgg
     1321 taccggtgtt attgccggcg gtcctgttcg tgccgttgtt gaagcagtag gtatcaagga
     1381 catcagaaca aaggctcttc gttccaacaa tccctgtaat gtagttagag ctactcttgc
     1441 aggtcttggc gcattaagaa atgctgaaga agttgctgca atccgcggta aatccgttaa
     1501 ggaaattctt taatagggag gttgtaaatt atgacaatta agttagtaaa gagccttatc
     1561 ggctctaaga aaaatcagat tgcaaccgcc catgctctcg gtcttaagaa aatcggtgat
     1621 gttactacac agcccgataa cgctcagact aagggtaagg tagcgaaaat cattcacctc
     1681 gtagaggtta gtgaagcata agcaaggagg tgcgaactaa tgaagttaca tgaactttct
     1741 cctgttgagg gttctaaaaa ggactcaaaa agaatcggca gaggccacgg ctcgggctgg
     1801 ggcaaaactt ccggtaaagg tcataagggc cagaaggctc gttcaggcgg cagcatccgt
     1861 cccggatttg agggcggtca gatgccttta cagcgtcgta tccctaaaag aggtttcaac
     1921 aacatttttg caaaagctgt agcagcagta aacctcagca ctttgaacag aaagtttgaa
     1981 gacggtgcga cagtagatat tgaggctctt gttaatgcgg gcgttgttaa gaacggcttt
     2041 gatgctgtta aaattcttgg caacggtaag cttgacaaaa aacttacagt aaaggtttct
     2101 gctttttccg aatcagctaa agctgcaatt gaggctgcgg gcggaaaggc agaggtgatc
     2161 taagtgttta aaaccatcaa aaatgcttgg tcactccccg atttaagaaa gaaaatttta
     2221 tttactctct taatcattgt tgtattcaga atcggctcgg ttattcaggt tccattcctg
     2281 gatactgcgg ctctcagaag tgttatgaat ccggatgact ggtctaacac aatgctttct
     2341 tatatgaata cactctcagg cggtgcattc tccaacgcaa cattgtttgc aatgggtatc
     2401 accccttata tcaacagctc aatcatcatc cagctcctct gcgttgctat tcctcctctg
     2461 gagagattgg caagagaagg cgaagccggc agacgcaaaa tcagtgctat cactcgttat
     2521 gtaacagtcg gtctcggtat tattcagggt actgcatatt acttctacct tctcaacagc
     2581 aaggtaacac tttacaacag cggttttgaa ctttggttct ctgctatcgt aatcatcctt
     2641 gtttttaccg caggtacggc ggttatgatg tggctcggtg aacagatcaa cagccacggc
     2701 atcggaaacg gtatttccat tctccttttt gcaggtatcg ttgctcagtt cccacagatt
     2761 atcaacacac tcggtcagta ctggaacctt gctgttaacg gcagcacaca gttcttcttc
     2821 ctcgtacctc tctgggtagt tctctttgtt gctgtcgtat ggattatcac attcatgcag
     2881 gacagcgaaa gaagaatccc gattcagtat gcaaagcgtg ttgtgggcag aaagatgtac
     2941 ggcggtcagt caagccacct tcctatcaag gttgctctcg gcggcgttct tccaatcatc
     3001 tttgcaagct caattctttc aattccaagt acaatcaacc tcttcctcaa gattcctgca
     3061 ggagaaggtt tctggggtgc attctttgca gcattcagca ccacaggctg gctgtacatg
     3121 gtactttatt tcttgttcat tataatgttc gcttatttct atacatcaat tcagtacaac
     3181 cctgttgaaa tggctaacaa tcttaaagct aacaacggca cagttcccgg tattcgtccg
     3241 ggtgctccga cagctgatta tatcagaaag attttgtcaa gaatcacttt aatcggtgca
     3301 atgttccttg ctgttatcgc tatgattccg cttatctacg gcgctgcttc cggaatggga
     3361 cagatgtcaa tcggcggtac atcaatcatc atcgtagttg gtgttgctct tgaaactgtt
     3421 aaacagctcg agtcacaaat gatgatgcgt cattacaaag gttttcttga ctaattgaga
     3481 aaaaaggagt ataatagatg aacattatca tgttaggtgc tccgggtgca ggtaaaggca
     3541 cacaggctgc tgtactttgt gaacactttg gtattcccac aatttcaacc ggaaacatga
     3601 tccgtgaagc gctgaaaaac ggcactgaga tgggcttgaa ggctaaatcc tttatggatg
     3661 aaggcaagct tgttcccgac gaagtagtaa tcggtattgt aaaggaaaga ctttctgccg
     3721 atgactgcaa gaagggtttc attcttgacg gttttccaag aactattcct caggctgagg
     3781 ctctggataa tatgggtgtt gagattcagc atgtcatcaa catcgacatt tctgatgaaa
     3841 aaatcatcaa ccgtatgtcc ggacgccgtg tttgcgaaaa ttgtggcaga cctttccaca
     3901 ttgtgacttt aaagcccaaa aaggaaggtg tttgtgacga ttgcggcggc acccttgttc
     3961 agcgcaagga cgaccacccc gataccgtgc ttgctcgtct cgaaacatat cacaaagaga
     4021 ctgagcccct cgtttcttac tatgagaagc agggtaagct tgttactgta aaaggacagg
     4081 acgatgtaga agctactaca aaggctatcc ttgaaggtat tgaggcttaa ttcatggttg
     4141 tagttaagac agcacgggaa ctttcaaaaa tgaaagatgc ctgcagaatt tctgccgagg
     4201 cattgcgtgt tgcgggagag gctgtaaagc ccggcgttac tacttatgaa atagataaca
     4261 ttgtccgctc atacatcgag aaacagggag cagttccctc gtttctcggt tacggcggct
     4321 ttcctgcgag tgcgtgcatt tccgttaaca atgtggttat ccatggcata cccagtaaag
     4381 aactcgtttt aaaagagggc gacatagtaa gcgtggatgt tggcgctttc tacgggggat
     4441 ttcacggcga caacgccttt acattcccct gtggcaaaat cagtgcagac gcacaggctt
     4501 tgcttgacgc cacaaaagag agtctgtatg agggaattaa acaggctctt gccttcaacc
     4561 gtgtcggtga cataggcaac gctgttcaga agtatgtcga agctcgcagt tactcggtgg
     4621 tacgagattt tgtcggccac ggcgtaggtg cgaaactgca cgaagaccca agcgtaccca
     4681 attacggcac tgctcacaga ggagtgcggc ttattccggg tatgactatt gccattgaac
     4741 ccatggtgaa ccagggcagg tgcaatgtgc gtgttcttga tgacaaatgg actaccgtca
     4801 ctgctgacgg aaagctttca gctcattttg aacatactgt tgcaataact cctgacggac
     4861 ctaaaattat gactttgtgc gattaaaggt gattttatga acacagtaaa aggcagtatc
     4921 gtaaaggctg tggcaggaag ggaaaagaac ggcttttttg ttgttctcga ctgcgattcc
     4981 gtttttgctt acattgcaga cggaaagcga agaaaggttg agaagcctaa aaagaaaaag
     5041 cttattcata tttctcctac aggaaccgtg atagagggtt caatcaagac caatccgcag
     5101 ataagaaaaa ttttgaacaa ttttagaaag agcgagggtt gacaatatgt caaaacagga
     5161 tgtaatcgaa attgaaggta ttgttaagga agctttaccg aacgcacagt ttaaggtaga
     5221 gttgccgaac ggtcacgtga tattagctgt tatttcaggc aaactgagaa tgaacttcat
     5281 tcgcatattg ccgggtgata aggtaaaggt ggaaatgtcc ccctatgacc tgacaagagg
     5341 cagaattacc tggagaacta aataattcgg gtaaaaacac gactttttag aaaggaatga
     5401 ttatacatga aagtcagacc ttcagtaaag aaaatgtgcg acaagtgcaa agtaatcaaa
     5461 agaaaaggta agattttagt tatttgcgaa aaccccaagc ataagcagcg ccagggttaa
     5521 tttacctaac gaaaacaaaa ctatatttta gaatttatat ggaggtgcaa ctatatggct
     5581 cgtatagcag gtgttgactt accgagagat aaaagagttg agattggttt gacctatatc
     5641 tacggtatcg gccgtaagac tgcagacgac attattgctg caacaggtgt taatccggac
     5701 actcgtgtca gagatttgac agaagaagat attgcaaagc tcagagaata catcgagcat
     5761 cactgccgcg ttgaaggtga ccttcgccgt gacatcgctt atgatattaa aagacttatt
     5821 gatattggtt gttaccgtgg cgttcgccac agaaaaggac ttcctgtaag aggtcagcgt
     5881 tcaaagacta acgctcgtac tcgtaagggt ccccgtaaaa ccatggctaa taagaagaag
     5941 taaggagggc aaaggactat ggcagcacca aagggtggta agaaggttat ccgccgtcgc
     6001 cgtgaacgca agcacattga aaaaggcgca gcgcatatcc agtctacatt taacaacaca
     6061 attgttacta tttccgatac acagggcaac gctgtttcat gggcaagcgc aggcgagctc
     6121 ggtttcagag gttcaaagaa gtcaactccg tttgccgctc agtcagcagc cgaaacagct
     6181 gcaaaggcag caatggaaca cggcatgaag tatgttgaag tatatgtaaa gggtcccggt
     6241 cagggtcgtg aagctgcaat ccgtgcattg cagactgcag gtcttgaggt aacaatgatt
     6301 aaggatgtta ctccaatccc gcacaacgga tgccgtcctc caaagagaag acgtgtatag
     6361 tattttaaaa ggaggaaaat tctactatgg ctaaaaatat gcagcctatc gctaagcgtt
     6421 gcagagctct tggcatttca cccgcagtta tgggttattc caagaagact tccaacagaa
     6481 atcccggcgg taagatgaga agaaaaaaga gcgaatacag catgcagctc actgagaagc
     6541 agaaggtaaa atttatctac ggcatcatgg agaagcagtt cagagcttac tacgaaaaag
     6601 ctgaaaaagc tgagggtaag acaggtgctg tattgcttac acttattgaa agaagacttg
     6661 acaacgttat cttcagactc ggtcttgcag ctacaagaag agaagcaaga cagcttgtta
     6721 gtcacagcca ctttaccgta aacggtaaga aggtaaacat tccttcatac cttgttaagg
     6781 ttggcgatgt aatcgaagtt aaggagaaga gccgttcaac tgcaagattc aaggcaatcg
     6841 cagagggcga aacatcttca attgtaacac caaagtggct tgagaagaac gaaaaccttc
     6901 tcggtggcaa ggttgttgca gctccccaga gagaagacat cgactttcct gttgaagaac
     6961 acctcatcgt tgagttgtac tccaagtaat cttatatttc acaggcagat tacacacaga
     7021 tgggctgctt ttgcagtcgc ataatgttta ggaggattcg cgaatgatag aaattgaaaa
     7081 gccaagaatt gaaaccgagg aattaaccga ggatggaaag tacggcaagt ttgttgttga
     7141 accgctcgag cgtggtttcg gcaacacact cggcaacagt ttgcgccgtg tgctgctttc
     7201 ctctcttgaa ggctgtgctg ttacatcaat caagattgac ggcgttcttc acgaattttc
     7261 aactattccc ggcgtaaagg aagacgttac ggaaattgtt cttaatatga aaagtcttgt
     7321 tgcaaagctc catgaaacaa gtcccaaggt tatagaaatt gcagccgagg gtccctgtga
     7381 ggtaaaggct gccgacatca aatgtgatga tgaggttgaa attctcaacc ctgaactcca
     7441 tattgctaca ctcggtgagg gtgctaagct caatatggaa atcacagttg acaagggcag
     7501 aggatatatt ccttcagagc gcaacaagca gttaagcggc aacaatgtta ttggcgtttt
     7561 gccgattgat tcaatttata ctcccgtgtt aaaggtaaac tatacagttg ataacactcg
     7621 tgtaggtcag attactgact atgacaagct cactttggat gtttggacaa acggagttat
     7681 caatgctcag gaggctgttt ctcttgcagc aaaggttctt accgaacacc ttaacctttt
     7741 tgttaacctt tccgacaaag cttcaagtgc tgaagttatg gttgagaagg atgacaaggg
     7801 taaggaaaag attcttgaaa tgacaattga agatcttgat ctttcagttc gttctttcaa
     7861 ctgcctcaag cgtgcaggca ttaacacggt agaggatttg atcaacaagt ccgaagagga
     7921 tatgatgaaa gtgcgtaacc tcggtagaaa atctctcgag gaagttgtac agaagctcaa
     7981 ttctctcggt tttagtcttc agaaggaaga cgaataactt cccaaagtct ttgacaggct
     8041 attctgtcaa attcttacca acaaactcaa cggtaaagga gtgaaagaaa tgccaggtac
     8101 aagaaaatta ggaagaacta ctgcacagag aactgcaatg cttcgtgcta tggttacttt
     8161 ccttttggaa aacggcaaaa ttgaaacaac tcttactcgt gccaaggaag ttcaggctat
     8221 ggcagagaag atgattacta cagctaaaga agatactctt cacaacagac gccttgtttt
     8281 ggcttttgtt actaaggaag atgttacaag caagctcttt aaggaaattg ctccaaaata
     8341 cagcgataga aacggtggct atacaagagt tactaaaatc ggtccccgtc gtggtgacgc
     8401 cgctgaaatg gcaattatcg agctcgttta attcttttta agttaataca agttcaattt
     8461 aactcccgac tttttagtcg ggagtttttg cgtttgtttc aaaatgtagg ctcttttact
     8521 accccaactt ttattataga gcccaaaaag ttcatcagca ggcgaacacg gtacggcgct
     8581 gtattgaaat tgtatgaaca actacaataa cacgctcggg cataaatgct ttttatttat
     8641 caaactgtat tcacgaacag aaaatggggt gcgccgtgct tttgtacaaa acaagacagg
     8701 ctgtgaagaa cgaagtctta aacttcattt cttcacagcc tcattttacg gttttaaaag
     8761 caattcaatt tttagttggc aacctaatct ttataagatg agctgagctt aaaaccgctc
     8821 gcaggcttac cctttttgtc gataaggttc ttactgtcgc ttgctctgca ggcaacttcc
     8881 cagtgataac ttggtgatgt tgagccgccg cctttaactt catatactac ggtcataaac
     8941 gcaccgccgt ttgtgtagtc ataaccgtca ccgatgttga cgccgcttaa agaaatctct
     9001 ccggcaatct ttatgttgag catagctccg tatttagcgg gtacagacat ttttgcactt
     9061 ttgcaggtaa gctttgatga atcatatttg attgttgcct gatagttttc gagaactgcc
     9121 ggtgttgtca gattgtatgt gcatacaatt gtgtcaccga cattgaatgt tttgccgccg
     9181 attttaagtg acttgctgtt gctgtcatca gattttgatg aatccgactt tgaagatgaa
     9241 ctgttattat ttttgttgct gttgttattt ttattgctgt tgttattatt cttattactg
     9301 ttgttattag ttttgcctga gtctgttttt gaagaactgt tgttagtagt tttgcttgtt
     9361 tcactctttt caataacgga actctgctga ctgtttgaat ttttgccgga agaactgccg
     9421 cctgagtttt cggagttatt gcctgaatcg cctgacttgc tgttgccgcc ggcgttgttg
     9481 tttacaggca cagcttttgt ttctttaaca tattcagtgt tgccctgagc gtctgttaca
     9541 acctcggtgt atgtttcaat ttttgtttca atctgagtat ctatgcgtgt ttcgtgaaca
     9601 atctttgttg ttttctgttc atccgaattt tctccgcaac cactcataac gcttgcagcc
     9661 atagttgaaa aagctacaag tgatgcaagt gccgcaataa gacaatagtt tttctttttg
     9721 ccgtaattac gcattatgcc acttccttaa tgtgatttaa aatttctgta agtgttaccg
     9781 tatctttacc ataatattat atcaaattaa tcaacgaaaa taaagaaatt tcgcaaaatt
     9841 gtaccgttat atattgaaaa attacttgta ttaattttat gcaaatggta caataatttc
     9901 gacggatgtg attaacaagt gaactttttg tgaacatatc taaaagttca ttattgatat
     9961 aattttttag gagtgattat agtgaggaca acaaaaaagg cgctttcaat tgtacttgct
    10021 ggtttgatga ccgttggcgg tatgtcggta ttttctgttt ctgccgctca aacatcaaca
    10081 ccgactcttt catttaaaac tcagaatgca ctttatgcac acgcagtaag cggttctgac
    10141 gattctgacg catgggttgc atggcaatgt aaacacaacg aggatatgga ggaattgaat
    10201 actaatagga agtatttctt ccttccaagc agtgtttcgt caacaagcgt tgagctttat
    10261 aacgcatatt caaaaagcgt tacggttaat aatgtaacta taccgtcagg cgagtcgagg
    10321 gaggtttcgt acacaattga taaggcaggc aatgttactg ccgacggaaa aacctattcc
    10381 ctcacattct taaagtcctc tgccgagtct gcaatctatg tgaacaacag caacgctgac
    10441 ggcaacggta aggaacttat ctcttatctt aacgaggata agagcaacta ttcctctgca
    10501 acaggcgcta ttgttgacag gaacggcaag attgataata cttcaatcaa gaaaatcaag
    10561 ggcagaggca actcaacatg gggcaaggct aagaagcctt acaacatcac atattccgat
    10621 aaggtttcaa tcggcggtat gtcaaagggc aaaaagttct ctcttcttgc aaactatcag
    10681 gatgattcac tcacaagaaa ccgttttctt tatgaccttg cagatgcagt aggaactcca
    10741 tatgcttcgg attcaagata tgttgatttc tactcagacg gttattactg gggttcatat
    10801 cagatgaccg aaaaaattga ggtgggtaag agtgcccttg tcaacgatat tgacgatact
    10861 gcataccttg acgctgacgg caatgtaaac aaggatttcc cgttcctttg tgaagttgat
    10921 tccggtgctg tagacggtga agattactat gtaaaatgtg atgacggtat caaggttaca
    10981 attaaagctc ctgagctttc ggaaggcgac aagggctata atgaagttaa gaactatgtc
    11041 cgtgaaaagt acaatgcttt ttacagagcc gctaaaaaag cagactccga cctttcacag
    11101 tacgctgatg ttgattcatg tgcaaagctg tggcttataa atgaactcgg taaaaactgg
    11161 gattccggtg tttcaagcgt ttactttgtt tataagcagg attctgacgg taactataag
    11221 ttcttcggct cacctgtatg ggactacgat aatgcgctcg gtaatgctgc aggcagtgct
    11281 tgggatcttc agaacttcgg tgttaaggat tacacacagt attcaggctg gtggtgcaga
    11341 ttcaaggaca gacagaaacg ctcccagaat agcagtaata tcattaacaa tatttcaaga
    11401 aacactcagg ttaataaagc tgccgtaaat atctggtttg agcagtttgt tcctgcaatc
    11461 aactattttg caggcaagac cacatcttac aagggctctg acgaattcta tacaaaaaca
    11521 cagtattttg accttctcaa ggacagcggt gcgatgaatt acaaaagcgg ctggcatata
    11581 agaacgagca gttggatttg tgaccacaca tcaatgaaca aggctgactt tgatatgaca
    11641 acaggtacat ataccgtaag taacactaag acaagctata atcagggcag ttttacggat
    11701 atgtataact atgcggctga ttggatgaca agccgtgcgg catggatcag cagtaagtgg
    11761 tttagcgagt acactccttc cgctaaaatc ggtgatgttg acggcgacgg cgaagttact
    11821 gtaatggacg caactcttgt tcagaagtac attgtaagtc ttgaaacact cacagacagt
    11881 cagctcaatg ttgccgatgt aaacggtgac ggtgaaatca gcgttattga cgcaacacaa
    11941 attcagaaga ttgtcgtaaa tcttgtataa tttaggcaca gaatcgggca ggaaactgcc
    12001 cgattttttg ttattgtttt gaaaattttt gtacaagcca cagaatgtaa aattttacaa
    12061 atcaaagatt gaaattgtgt tgtgaataat atataatgag tgtaatcaaa atttttacac
    12121 agatttatac atatgcacaa taacagaata taagcatttt aaaaggacgg agaaaacatg
    12181 agaaaaacta agatagtttg taccatcgga cctgcctgcg attcaaaaga aatgatgcgt
    12241 aaaatgattg acgcaggtat gaatgttgcc agaatcaata tgtcacacgg cgtttatgac
    12301 cggcttgagg ttacaatcaa aaaccttaaa gaggcaattg cagagagtgg tcaaaatgtt
    12361 gcaattctgc tcgatacaaa gggtcctgag gtcagagtcg gca
//
LOCUS       DAOMVE010000042        12088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1210_length_12088_cov_3.915150, whole genome shotgun sequence.
ACCESSION   DAOMVE010000042 DAOMVE010000000
VERSION     DAOMVE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12088
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1210_length_12088_cov_3.915150"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLS64_03740"
     CDS             <1..672
                     /locus_tag="PLS64_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42975.1"
                     /translation="YAQPIYNQGGYGQPIGVPPAVEYQKSYFDGTGFQLMGWNILCSI
                     IIFITFGFGAAWASCLKLRWETSHTVVNGKRLYFNGTAAQLFGKMIAWEIIVGLILSI
                     LPIMAITTTKYSYNILPNLIVAIIVDAIISMFIVPFYSVYIKKWVIKHTTFVDDRGSA
                     MPTAPYYQNQVQYQNQSQYQNPQTYNGAANDNVATVNLKRCPFCYTDNSEDSEFCQNC
                     GRKLD"
     gene            971..1441
                     /locus_tag="PLS64_03745"
     CDS             971..1441
                     /locus_tag="PLS64_03745"
                     /inference="COORDINATES: protein motif:HMM:NF015550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HRL42976.1"
                     /translation="MTGREKKFCSLFLGSGNSELAAEKAGYTGDCEQKGEELICRPEI
                     SAELERLSRLREKSLANMAAAGYQRLAFGSICDAISLLYKSDPSKEDLEGMDLFLVSE
                     IKRPKDGSMEIKFFDRLKALEKLGAGGEHETGAKQLFDAISNSARAVNDKENGN"
     gene            1431..2690
                     /locus_tag="PLS64_03750"
     CDS             1431..2690
                     /locus_tag="PLS64_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HRL42977.1"
                     /translation="MEIKAFSKKQLTVLSWWNRESAFRDRDAIICDGAVRSGKTFCMS
                     LSFILWSFYDFANSDFALCGKTIRSLRRNMITPVIPILKSLGFKCEEKLSQNILTVSV
                     NGVMNRFYLFGGKDESSASLIQGMTLSGVLFDEVALMPRSFVEQALARCSVSGSRFWF
                     NCNPEFPEHWFYREWIKKCGDKNALYLHFTMQDNPSLKPEVIKRYESLYSGVFYERFV
                     KGRWVAVFGAVYPFMDNERMYCDIPSDIESWAVSCDYGTVNPASFGLWGRKNGVWYRV
                     DEYYFNSRTQGFQKTDEEHYDGLEKLIDGRKIECVIVDPSAASFIEVIRRHGKYTVVS
                     AENNVINGIRQTSQALKDRKIRICKNCRAARREFSLYRWDGSGRSDAPVKENDHAMDD
                     IRYFVATKIYGCDGFFAVATKRQEETA"
     gene            2687..3841
                     /locus_tag="PLS64_03755"
     CDS             2687..3841
                     /locus_tag="PLS64_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HRL42978.1"
                     /translation="MRLGRKNKKTESIKTVQTVLRETRNNSPIFSRFAVQTRTERQLY
                     TTLRESVPIIDAALCKIIRLIGGFKIVTSSAESQKIADSFVKNVRTNGEMTGLESFVL
                     CYLDSLLTYGQAVGEIVPDSGGEGICALYNASLDDVEIRADSSPLKLAVYTLGNGTAE
                     EPKHPERIFATLLNPKPGTVCGTSILSGLPFVSSILLRIFESVKTNWERVGDIRFAVT
                     LNPDSNGSAVSRENAQAVADEWKKAMRSDSVCDFVSVGDVSIKVIGAESDMPDCDIPV
                     RHILEQIIAKLGIPPFLLGISWSSTERMSEQQADILTSELAYYRTVLEPVITKIVSAH
                     LKMCGYNDSFKIEWDKINLQDAVELSQARLNNANAMNIERQIGAEVQNEG"
     gene            3831..4826
                     /locus_tag="PLS64_03760"
     CDS             3831..4826
                     /locus_tag="PLS64_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42979.1"
                     /translation="MKDNKLIKSGVSGVVDGENQTVGDDELELINRFTRRNLAKNEVY
                     AFSVVLCDNDVDRDGERFTTDSLYELEKLFVGKTGIIDHNPSAKNQTARIFSCKVEKI
                     DGQKTALGDDYYRLKARAYLPVCESNRDIILAIDSGIIKEVSVGCAVGRVVCNVCGED
                     ISMCTHKKGEVYGSKLCCGELVNPYDAYEWSFVAVPSQKRAGITKGHKIFGKENDMEK
                     ILKAIENKKAFTLDESDSRKLCEYIDGLKKSAKDGVLYRESLTRDVVGLAAFVQPDIS
                     GETMESVAKSMTIEQLREFKSAFEKKKKAAFEPVPQLYCKQDKRNNTVENGQFSI"
     gene            4840..5175
                     /locus_tag="PLS64_03765"
     CDS             4840..5175
                     /locus_tag="PLS64_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42980.1"
                     /translation="MNVNFNGFGENAATFIADETLTEAGVPVKMKDNGTVAKCDASEN
                     FCGVCVSVRGGYAVVQLSGYVKVKSDKKIAVGYKKLSATADGGVSVTTTGREYLVLDS
                     TDTSVGFIL"
     gene            5194..6201
                     /locus_tag="PLS64_03770"
     CDS             5194..6201
                     /locus_tag="PLS64_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HRL42981.1"
                     /translation="MANFENITIEKGMYQTKGGISGALEKLDPSENYRGTALEGLDAF
                     SRQLKRFDIKVKGRNSDCVEKFFQSSNSAALFPEYVSRAVMQGMERADILPNLVATVT
                     DIEGMDYRSIASVPSEDDKSLKLVGEGAKIPQTEVKTRENLVKLHKRGRMLVASYEAL
                     RFQRLDLFTVTLNQIGAYIARAQLKDAIDVLVNGDGNENPAGTLNVATGGKVTYEDLL
                     KLWTELAPYELNTILASTPEMQKILSLSQLQDSNAGLDFQATGRMITPLGASLLHTPE
                     LEGGKIIGLDKNCALEMVQAGGVVTDYDKLIDRQLERAAVTCTAGFSKIFTEASKVMS
                     C"
     gene            6214..6567
                     /locus_tag="PLS64_03775"
     CDS             6214..6567
                     /locus_tag="PLS64_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42982.1"
                     /translation="MNIANITKRFALYSGIDGAEAYKWKSIIDDAVVYVNSIVTKENL
                     SEDDELRLENLCAVYAFKLYSLCNDDSISSFSAGDLKISSSADGESRAEKLWREYADK
                     SQDLIGREKFLLGVI"
     gene            6564..6923
                     /locus_tag="PLS64_03780"
     CDS             6564..6923
                     /locus_tag="PLS64_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42983.1"
                     /translation="MMNISPSIGKILNRYGCDVTVKNGGKSVRTKAFISPLRYNSNQN
                     YDSVRHKLGMRKTKLFLFIAPPDVLLDSEKSVIESENGKYTVKRCEKYYVKDNPIYVR
                     AVLCAYREETRDDFESN"
     gene            6910..7341
                     /locus_tag="PLS64_03785"
     CDS             6910..7341
                     /locus_tag="PLS64_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42984.1"
                     /translation="MNRIEKQVDRIIAGLKVNEALKNVRFIREYGSDEAPSPVNGMIA
                     VVSVRDMSTEKSYIGGYLSPSIKGESYNAGVEIRVYAPATENGSGLSEVVSEILLGLK
                     TADAEKTITHSEAASIEFDPDMNAIYRTVSFNMEFCLCEEV"
     gene            7342..7707
                     /locus_tag="PLS64_03790"
     CDS             7342..7707
                     /locus_tag="PLS64_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42985.1"
                     /translation="MDGFEFENCGNAMLKCEGKILGGVEKATCTRKNSFTEIKEFFND
                     KPVERIVSNEWELTFVMKITDKTPFLERDSFKSLELDLAKKKIIYTDCKVLEFSSVTQ
                     GSGSILATVKISADERKII"
     gene            7704..7853
                     /locus_tag="PLS64_03795"
     CDS             7704..7853
                     /locus_tag="PLS64_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42986.1"
                     /translation="MNDKNSDELYRLAESENGGKDTEMFGEFLERESRRYSRRLDEEE
                     EAKSL"
     gene            7850..8365
                     /locus_tag="PLS64_03800"
     CDS             7850..8365
                     /locus_tag="PLS64_03800"
                     /inference="COORDINATES: protein motif:HMM:NF018818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HRL42987.1"
                     /translation="MKPVPMKFGEYVWHHNPQNISFECDRSVAEMKSPFGESSVQDMG
                     RKNMKISGSGQLYGENCAEQFERLFEVFRNSGKEVLSVPNLPSIYAVFEKLEIKGEPK
                     PNVLEYSFVFREVMEKKQKTVITYFDCENGQTLWDIAYKTGVKIDELVRLNPDVKFPD
                     ENLGTRRVKLC"
     gene            8359..9315
                     /locus_tag="PLS64_03805"
     CDS             8359..9315
                     /locus_tag="PLS64_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42988.1"
                     /translation="MLTYFFTDKNGKRCEIKNVLTAEISADVDVPADELVMTVPYDEK
                     FGNADMLEAYDGKSLVFVGQADEIVSIVRTDGAIVRLSARSLAGRLLDNEAEPVTYVN
                     PAAKFIFERHLKPFGIVGYDGDEHPFMGTIKIEKGMTEWQVLEKFCNGRYGKSPRITG
                     AGFALMCGTYGGAKPIVFGRNGVGYTSLREYIKPCKVISQIKLRTEEYGGYKSVISNK
                     CVADRIKRVRYVNAFLDNNAVKTADRMIENGNRQSFEIMLECAECLCGVVGRRAVIDD
                     SLIGKREGLVVKSIKYSLGKNGESTTVVLGKENGDVADELHN"
     gene            9299..9619
                     /locus_tag="PLS64_03810"
     CDS             9299..9619
                     /locus_tag="PLS64_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HRL42989.1"
                     /translation="MNYITKNSITAPKAEKGGVKSSGNTVSVDSSEEHRGIKCCVPYG
                     FASVVPVGESAVVLPLANGEVSLGVLAKNVELDEGEVMLSSKGGASIVLKNDGRVLIN
                     GKAV"
     gene            9621..9950
                     /locus_tag="PLS64_03815"
     CDS             9621..9950
                     /locus_tag="PLS64_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42990.1"
                     /translation="MRDTMIKNGDIVIGSSGNTVLLEGSDAKFQQAVLCISAKLGGFV
                     YDRNFGSKVLLQDKTLSAKQTELLANESLAKMKNTYASVKSVGRQITIDLTVDDITRK
                     VQINGNL"
     gene            9937..10995
                     /locus_tag="PLS64_03820"
     CDS             9937..10995
                     /locus_tag="PLS64_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HRL42991.1"
                     /translation="METYDEIYGRMKNAYEHETGDSFNEVSDIAIRLKVLAGEIFKLQ
                     TNLEWWKRQMFAVSASGECLDKLASQRGIERKKAMKSTGEITFNISQPCSHDIVIPKG
                     CVVATADLVPIRFVTTEDEEISAGNTLVSVYAEAEQAGSNGNIGLGCAVVPVSVPTEI
                     ETVTNREKFTGGCDAETDDELRKRIRDTYINTSNGTNAAYYEQLALTVDGVAKASAIG
                     KARGVGTVNVYVTGADASLGTNVVAKVQSLLEKQRELNVDVIVANAQRTACNMSVVAY
                     AEDGYSSGEVKELLKNAFAEYVNSIPIGGTFRLSELGARLIDTGCITNYNWNTDMQDV
                     TVAKSQCFTVGTVTIGVK"
     gene            10995..11474
                     /locus_tag="PLS64_03825"
     CDS             10995..11474
                     /locus_tag="PLS64_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42992.1"
                     /translation="MNSFDSMKTKLESTGLYKVTAKSNIRAELLAYAEGLNTEFDMLE
                     TMERELFIDTAENCGITERERFVGKINADYPLEKRREMLKISEQKVGGKCTPDDFKRI
                     VRGYGVENFTIAEAPTRNRVDIKISDTKTDAEKKLIEKRVKADFPLHLNVIISYVNA"
     gene            11792..11992
                     /locus_tag="PLS64_03830"
     CDS             11792..11992
                     /locus_tag="PLS64_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL42993.1"
                     /translation="MNKIAEFIKQKRKEAGLTQEDFAIRSGLGLRFVRELEQGKETVR
                     MDKVNQALSMFGMEAVPGKKDD"
BASE COUNT         3580 a         2158 c         3193 g         3157 t
ORIGIN      
        1 tatgctcagc ctatttataa tcaaggcggt tacggtcaac cgataggcgt tccgccggct
       61 gttgaatatc agaaatcgta ttttgacgga acaggttttc aacttatggg ctggaacata
      121 ctttgttcaa ttattatttt tataacattc ggttttggtg cggcatgggc gtcctgcctt
      181 aaactgaggt gggaaacaag tcacacggtt gtgaacggaa aaagacttta ttttaacggt
      241 acagccgctc agcttttcgg aaaaatgatt gcgtgggaga ttattgtcgg tctgattctt
      301 agtattcttc ctattatggc aattacgacc acaaaatatt catataatat tttgccgaat
      361 ttgatagtcg caattattgt tgatgctata atttcgatgt ttattgttcc gttttattct
      421 gtttacatta aaaaatgggt aataaaacat acgacattcg ttgatgacag aggttcggca
      481 atgcctactg caccgtatta tcagaatcag gtgcaatatc aaaatcaatc gcagtatcaa
      541 aatccgcaga catacaacgg tgcggctaat gacaatgttg caacggtaaa tttaaaacgc
      601 tgtccgtttt gttacacgga taattctgag gacagcgaat tttgccagaa ttgcggaaga
      661 aagcttgact gacaattata ttaatcggtt ttacctcaca aaagtggtaa ataatattaa
      721 gcaaagggtt tgcacataca gattttctgt atgtgtaaat ccttttttta ttatctgatt
      781 cggaaaagac ggctttctga gggggatcag gcggatatct ttgactattg atttttggtt
      841 gtgaataatt atttttaaaa atatttttga aaattgcatt aaagtatgca atttttttga
      901 attttgcagg tataagtgag gggcgttgat aaagcctctc gaaaaacgga ggtgacgaga
      961 tatcagaaaa ttgacaggta gggaaaagaa gttttgcagt ttatttctcg gttcgggaaa
     1021 ttccgagctt gccgcagaaa aggcaggcta cacgggggat tgtgagcaga agggggaaga
     1081 gcttatctgc cgtcccgaaa tttcagccga gcttgaacgg ctgtcacggc taagagaaaa
     1141 atcccttgcc aacatggcgg cggcagggta tcagcggttg gctttcggga gcatttgcga
     1201 tgcaatttct ttgctttaca aaagtgatcc gagcaaggag gatcttgagg gaatggattt
     1261 gttccttgtg tcggagatta aaagaccgaa ggacggttca atggaaatca agttttttga
     1321 caggctaaag gcacttgaaa aactcggtgc cggcggagag catgaaacag gcgcaaaaca
     1381 gctttttgac gccatttcaa acagcgcaag ggcggtgaat gacaaggaaa atggaaatta
     1441 aagctttttc taaaaaacag cttaccgtgc tttcgtggtg gaacagggag tcggcttttc
     1501 gtgacaggga cgcaatcatc tgtgacggtg ctgtgcgcag cggaaagaca ttttgtatgt
     1561 cgctgtcttt tattctgtgg agcttttacg attttgcaaa ttcggacttt gcactttgcg
     1621 gaaagacaat ccgttcttta aggcgaaata tgattacgcc cgtgattccg attttgaaat
     1681 cactcggttt taagtgtgaa gaaaagctgt cgcagaatat tttgaccgtg agcgttaacg
     1741 gagtgatgaa caggttttat cttttcggag gcaaggacga gtcatccgca tcgctcattc
     1801 agggcatgac gctttcgggt gtgctttttg acgaggttgc gttgatgccg aggtcgttcg
     1861 ttgaacaggc attggcgaga tgttccgtgt cgggttcaag attttggttt aactgcaatc
     1921 ccgaatttcc tgagcattgg ttctaccgtg agtggattaa aaagtgcggt gacaaaaatg
     1981 cgttgtatct gcactttaca atgcaggaca atccgtcttt gaagcccgag gttatcaagc
     2041 ggtatgaaag tctgtattcg ggtgtgtttt acgagaggtt cgtaaaaggc agatgggtag
     2101 ccgttttcgg tgcggtttat ccgtttatgg acaatgaaag gatgtactgc gatattccgt
     2161 cagatattga aagctgggcg gtatcgtgcg attacggtac tgtaaatccc gcatcattcg
     2221 gtttgtgggg cagaaaaaac ggtgtgtggt acagggttga cgaatactac ttcaactcac
     2281 gcactcaggg ctttcaaaag accgacgagg agcattatga cggacttgaa aagctgattg
     2341 acgggcggaa aatcgaatgt gtgattgtcg atccgtctgc cgcaagcttt attgaggtta
     2401 taaggagaca cggaaaatac acggttgtgt cggctgaaaa caatgttatc aacggcataa
     2461 gacagacttc gcaggctttg aaggacagaa aaatcagaat ctgcaaaaat tgcagagccg
     2521 caagaaggga attttcgctt taccgttggg acggttcggg gcgcagtgac gcacctgtta
     2581 aggaaaacga tcatgcaatg gacgacataa gatattttgt cgctacgaaa atttacggtt
     2641 gtgacggatt ctttgccgtt gcaaccaaaa gacaggagga aacagcttga ggcttggcag
     2701 aaaaaataaa aagaccgaga gcataaagac ggtgcagacc gttttgagag aaacgagaaa
     2761 taattcgccg attttctcac gatttgccgt tcagacgaga acggaaaggc agctgtacac
     2821 aactttgcgt gagtctgtgc cgattattga tgcggcactc tgcaaaatta tcagacttat
     2881 cggcggattc aaaattgtga cttcatcggc tgaaagtcag aagattgccg acagctttgt
     2941 taaaaatgtc cgcacaaacg gtgaaatgac gggacttgaa agttttgtac tttgctatct
     3001 tgattcgctt ctcacctacg gacaggcggt cggtgagatt gttcccgata gtggcggtga
     3061 aggaatttgc gcattgtaca atgcaagcct tgatgatgtt gaaatcagag cggattcttc
     3121 tccgctgaag cttgcggttt acacactcgg caacggtaca gccgaagaac ctaagcatcc
     3181 ggaaaggatt tttgcaaccc tgcttaatcc aaagccgggt actgtgtgcg gtacttccat
     3241 actcagcggt ttgccgtttg tcagctcaat acttttgagg atttttgagt cggtaaaaac
     3301 aaactgggag agggttggcg atatccgttt tgcggttact ctcaatcccg attcaaacgg
     3361 ttcggctgtg agcagagaaa atgcacaggc ggttgccgat gagtggaaaa aggcgatgag
     3421 aagcgacagc gtgtgtgatt ttgtgtcggt cggcgatgtc agcattaagg ttatcggtgc
     3481 tgaaagcgat atgcccgact gcgacattcc cgtaaggcat attcttgagc agattattgc
     3541 aaagcttggt attccgccgt ttttgctcgg catttcgtgg tcgagtacgg agagaatgag
     3601 cgaacagcag gcggatattc tcacaagcga gcttgcctac taccgcaccg tgcttgaacc
     3661 cgtgattaca aaaattgtgt cggctcatct taaaatgtgc ggttataacg acagctttaa
     3721 gattgagtgg gacaagatta atcttcagga tgcggttgag ctttctcagg caagacttaa
     3781 caatgcaaat gcgatgaaca ttgaaagaca gattggagcg gaggtgcaga atgaaggata
     3841 acaaacttat taaaagcggt gtttcgggcg ttgttgacgg tgaaaatcag actgtcggcg
     3901 atgatgaact tgaactgatt aaccgcttta caaggcgaaa tcttgcaaaa aatgaggtgt
     3961 atgcgttttc ggttgtgctg tgtgacaacg atgttgaccg tgacggcgaa cgctttacaa
     4021 cagattcgct ttatgagctt gaaaagcttt ttgtcggcaa gacgggaatt attgaccaca
     4081 atccgagtgc caaaaatcag acggcaagaa ttttcagctg taaggttgag aaaattgacg
     4141 gtcagaaaac ggctttgggt gacgattact acaggctcaa ggcaagggca tatcttcccg
     4201 tttgtgagag caacagggat attattcttg cgattgacag cggaattatc aaggaagtaa
     4261 gcgttggctg tgccgttggc agggttgtgt gcaatgtgtg cggtgaggac atctcgatgt
     4321 gtactcacaa aaagggcgag gtttacggct caaagctttg ttgcggtgaa cttgtgaacc
     4381 cgtatgacgc atacgaatgg agctttgttg ccgtgccgtc acaaaagagg gcaggcatta
     4441 cgaaaggtca caaaattttt ggaaaggaaa atgatatgga gaaaattctt aaagccattg
     4501 aaaacaaaaa ggcttttaca cttgatgaga gcgacagcag aaagctgtgc gaatatattg
     4561 acgggcttaa aaagtcggct aaggacggtg tgctgtaccg tgaaagcctt acccgtgatg
     4621 ttgtgggact tgccgctttt gttcagcctg acatttcggg ggaaacaatg gagagcgttg
     4681 caaagagcat gacaattgaa cagctcagag aatttaagtc agcatttgaa aagaaaaaga
     4741 aagcagcttt tgagcctgtt ccgcagcttt actgcaagca ggacaagaga aataacaccg
     4801 tggaaaacgg tcagttcagt atttaacgga ggtattatta tgaatgtaaa ttttaacgga
     4861 ttcggcgaaa atgccgcaac atttattgca gacgaaacac ttacagaggc aggcgtgcct
     4921 gttaagatga aggacaacgg cactgttgca aaatgtgacg caagcgagaa cttttgcggt
     4981 gtgtgcgtaa gcgtgagagg cggttatgcg gttgttcagc tttcgggcta tgtaaaggtt
     5041 aagagcgaca agaaaatcgc cgtcggctac aaaaagcttt ctgcaacggc agacggcggc
     5101 gtgtcggtta caacaaccgg cagagagtac cttgtgcttg actcaacaga cacttcggta
     5161 ggatttattc tttgataagg ggaggaagat attatggcaa attttgaaaa tattacaatt
     5221 gaaaagggta tgtatcagac aaagggcgga atttcgggcg cacttgaaaa gcttgatccg
     5281 tcagaaaatt acagaggtac tgcacttgag ggacttgacg cattttcccg tcagctcaaa
     5341 cgctttgaca ttaaggttaa gggcagaaac agcgactgtg ttgaaaagtt ttttcagagt
     5401 tcaaactctg cggcactttt ccccgaatat gtgagcagag ccgttatgca gggcatggag
     5461 agagcggata ttctcccaaa tcttgtggca accgtgacag acattgaggg tatggattac
     5521 cgcagtattg catctgttcc gagtgaggat gacaagagtc ttaaactcgt cggcgagggt
     5581 gcaaagattc cgcagactga ggttaagaca agagaaaacc ttgttaagct tcacaagcgt
     5641 ggcagaatgc ttgttgcatc atatgaggcg cttcgctttc agcgtcttga cctctttacc
     5701 gtaacactca atcagattgg cgcatatatt gcaagagcac agcttaaaga tgcgattgat
     5761 gtgcttgtga acggtgacgg caatgaaaat cccgccggca cacttaatgt tgcaacaggc
     5821 ggcaaggtta catatgagga ccttttaaag ctctggacag agcttgctcc gtatgaactc
     5881 aacacaattc ttgcgtcaac ccccgaaatg cagaagattc tttcgctctc tcagcttcag
     5941 gattcaaacg caggtcttga ttttcaggct acgggcagaa tgattacacc tctcggtgca
     6001 agccttcttc acactcccga gcttgagggc ggtaagatca tcggtcttga caaaaactgt
     6061 gcgcttgaaa tggttcaggc aggcggtgtt gttacagatt acgacaagct tattgaccgt
     6121 cagcttgaaa gagccgcagt tacctgtacc gccggttttt caaaaatctt tacagaggcg
     6181 tcaaaggtga tgagctgtta aggagggatt gccttgaaca ttgcaaacat tacaaagcgt
     6241 tttgccttat acagcggtat tgacggtgct gaagcataca aatggaagag cattattgac
     6301 gatgccgtgg tgtatgttaa ttcgattgtt acgaaggaaa atctttcgga agatgacgaa
     6361 ttaagacttg aaaacctgtg tgccgtttac gcttttaagt tgtattccct ttgcaatgat
     6421 gacagcattt cttctttttc cgcaggtgat ttgaaaattt catcatctgc ggacggcgaa
     6481 agccgtgccg aaaagctgtg gagggaatat gccgacaagt cgcaggacct tatcggcaga
     6541 gaaaaatttt tgcttggggt gatatgatga atatttcacc gtctatcggg aaaatattaa
     6601 acagatacgg ctgtgatgtt accgttaaaa acggcggtaa atcggttagg acaaaggcct
     6661 ttatttcacc tttgagatac aacagcaatc agaattatga cagtgtacgg cataaactgg
     6721 gtatgagaaa aacaaagctg tttttattta ttgcaccgcc cgatgttctg cttgattcgg
     6781 aaaaaagcgt aatagaaagt gaaaacggta aatatactgt taaaaggtgc gaaaaatatt
     6841 atgtgaagga caatccgatt tatgtaaggg ctgttctgtg tgcatacaga gaagaaacga
     6901 gggatgattt tgaatcgaat tgagaaacag gttgaccgta ttattgcagg attaaaggta
     6961 aatgaggctt tgaaaaatgt cagatttata agagaatacg gctctgatga agcaccgtca
     7021 cccgtgaacg gaatgattgc cgttgtgtcg gtgagagata tgtcaacgga gaaaagctat
     7081 atcggcggat acctttcgcc gtctatcaag ggtgaaagct acaatgcagg agttgaaatc
     7141 agggtgtatg ctcctgcaac cgagaacgga agcggtcttt cggaagtggt aagtgaaatt
     7201 cttctcggac ttaaaactgc cgatgcggaa aagacgatta cccacagcga ggcggcgtca
     7261 attgaatttg atcccgatat gaacgcaatt tacagaacgg tgagttttaa tatggaattc
     7321 tgtctttgcg aggaggttta aatggacggc tttgaatttg aaaattgcgg aaatgccatg
     7381 ttgaaatgtg agggaaaaat tctcggcggc gttgaaaagg caacctgtac aagaaagaac
     7441 tccttcacgg aaatcaagga atttttcaat gacaagcccg ttgaaaggat tgtttcaaat
     7501 gaatgggaac ttacctttgt gatgaagatt acggataaaa ctccgttttt ggagcgtgac
     7561 agctttaaga gtcttgaact tgaccttgca aagaagaaaa tcatttacac ggattgcaaa
     7621 gtgcttgaat tttcaagcgt tactcagggc agcggaagta ttcttgcaac cgtgaaaatc
     7681 agtgccgacg agaggaaaat tatatgaatg ataaaaattc agacgaactt tacaggcttg
     7741 cggagtctga gaacggcggt aaagataccg aaatgttcgg tgaattcctt gaaagggaaa
     7801 gccgtcgtta cagtcgcaga cttgacgaag aagaggaggc gaaaagccta tgaaaccggt
     7861 gccgatgaaa ttcggtgaat atgtgtggca tcacaatccg cagaatatca gctttgaatg
     7921 tgacaggagc gttgcagaaa tgaaaagtcc gttcggcgaa tcttccgttc aggatatggg
     7981 gcggaagaat atgaaaatca gcggttcggg acagctgtac ggcgagaatt gtgcagaaca
     8041 gtttgaaagg ctgtttgagg tgttcagaaa cagcggaaaa gaagtgctct ccgtgccaaa
     8101 cctgccgagc atttatgctg tgtttgaaaa gcttgaaata aagggcgagc caaagccgaa
     8161 tgtgcttgaa tacagctttg tgttccgtga ggttatggag aaaaagcaga aaacggtaat
     8221 tacatatttt gactgtgaaa acggacaaac cctgtgggac attgcataca aaacaggggt
     8281 gaaaattgac gagcttgtgc gactgaatcc cgatgttaag ttccccgatg aaaacctcgg
     8341 aacaaggagg gttaagctgt gctgacttac ttttttactg ataaaaacgg caaaaggtgt
     8401 gaaattaaaa atgttctcac ggcagaaatt tcggcagatg tcgatgtgcc tgccgatgag
     8461 cttgtgatga ctgtgccgta tgacgagaag ttcggaaatg ccgatatgct tgaggcttat
     8521 gacggcaagt cgcttgtgtt tgtgggacag gctgacgaga ttgtcagcat tgtgagaacc
     8581 gacggtgcga ttgtaaggct gagtgcaaga agtcttgccg gaaggcttct cgacaatgag
     8641 gcagagcctg ttacatatgt gaacccggcg gcaaagttca tttttgaaag gcatttaaag
     8701 ccgttcggaa ttgtcggata tgacggtgac gaacatccgt ttatgggcac aatcaaaatt
     8761 gaaaagggta tgaccgagtg gcaggtgctt gaaaaattct gcaacggcag atacggcaaa
     8821 agtccgagaa ttacgggtgc gggatttgct ttgatgtgcg gaacttacgg cggtgcaaag
     8881 ccgattgtgt tcggcagaaa cggagtaggc tacacatctc tccgtgagta cataaagccg
     8941 tgcaaggtga tttcgcaaat caaactacgc accgaggaat acggcggtta caagagcgtt
     9001 ataagcaaca aatgcgttgc cgacaggatt aaaagggtga gatatgtaaa cgcttttctc
     9061 gacaacaatg cggtaaaaac agccgacaga atgattgaaa acggcaacag gcagagcttt
     9121 gaaataatgc ttgaatgtgc agaatgtctg tgcggagttg tcggcagaag ggctgtgatt
     9181 gatgactctc ttatcggaaa aagagagggc ttggttgtga aaagcattaa atattcactt
     9241 gggaaaaacg gtgaaagcac aacggttgtg cttggaaagg agaacggcga tgtggctgat
     9301 gaattacata actaaaaatt cgattaccgc cccgaaagcc gaaaagggcg gtgtgaaaag
     9361 ttcgggaaac acggtttcgg tggattcctc ggaagaacac agggggataa aatgttgcgt
     9421 gccgtatggc tttgcaagcg ttgttcccgt gggagagtcg gcggttgtct tgccgcttgc
     9481 taacggtgaa gtgagccttg gcgtgcttgc aaaaaatgtt gaacttgatg agggcgaggt
     9541 tatgctctcg tcaaagggcg gagcgagtat tgtgctgaaa aatgacggca gggttcttat
     9601 caacggcaag gcggtgtagt atgagggata cgatgattaa aaacggtgat atcgttatcg
     9661 gctcttcggg caatacggta ttgcttgagg ggagtgacgc aaaattccaa caggctgtgc
     9721 tttgcatttc ggcaaaactc ggcggatttg tctatgacag aaatttcggt tcaaaggtgc
     9781 ttttgcagga caaaacactc tcggcaaagc agactgaact gcttgccaat gaatcgcttg
     9841 caaaaatgaa aaatacctat gcaagcgtta agtcggttgg cagacagatt acgattgacc
     9901 ttacggttga cgatattaca aggaaggtgc agataaatgg aaacctatga tgaaatttac
     9961 ggcagaatga agaatgccta tgagcatgaa acgggtgaca gctttaacga ggtgagcgac
    10021 attgcaatca ggctcaaggt gcttgccggc gagattttta agctacagac gaatcttgaa
    10081 tggtggaaaa gacagatgtt tgcagtgagc gcaagcggtg aatgccttga caaactcgca
    10141 tcgcagagag gtattgaacg caaaaaggcg atgaagtcaa cgggcgaaat taccttcaat
    10201 atttctcagc cgtgcagtca cgatattgta attccaaagg ggtgcgttgt ggctactgcc
    10261 gaccttgtgc cgatacgatt tgttacgacc gaggatgaag aaatcagtgc cggcaacacg
    10321 cttgtgagtg tttatgccga ggctgaacag gcgggaagta acggtaatat cgggcttggt
    10381 tgtgcggttg ttcccgtgag tgtgccgaca gagattgaaa cggttacaaa ccgtgagaaa
    10441 tttacgggcg gttgcgatgc cgaaacggac gatgaacttc gcaaacgcat aagagataca
    10501 tatataaaca cctccaacgg cacgaatgcg gcatattacg aacagcttgc actcacggtt
    10561 gacggtgttg caaaggcgag tgccatcggc aaagcgaggg gcgtaggtac ggttaatgtc
    10621 tatgttacgg gtgcggatgc atcattgggt acgaatgttg ttgcaaaggt tcagtcgctt
    10681 ttggaaaagc agagagagct taatgttgat gttattgtgg cgaatgccca gcgtaccgct
    10741 tgcaatatga gtgttgttgc ctatgcggag gacggatatt cttcaggtga agtcaaggag
    10801 ttgctcaaaa atgcctttgc ggaatatgtg aattcaatcc ctatcggcgg aacattcaga
    10861 ttgtcggaac tcggcgcaag actgattgac acgggttgta taaccaacta caactggaac
    10921 acggatatgc aggatgtgac ggtggcaaag tcgcaatgtt ttactgtcgg tacagttacg
    10981 attggggtga agtgatgaac agctttgatt cgatgaaaac caaattagaa agtacggggc
    11041 tttacaaagt tacggcaaaa tcaaatatca gagcggaact tttggcatat gcagagggtt
    11101 tgaacacgga atttgatatg cttgaaacta tggaacggga gttgtttatt gacacagcgg
    11161 aaaactgcgg aattaccgaa agggaaagat ttgtcggtaa aatcaatgcc gattatccgc
    11221 ttgaaaaacg aagggaaatg cttaaaatat ctgagcagaa ggttggcgga aaatgcactc
    11281 ccgacgattt caaaagaatt gtcagaggtt acggtgtgga aaattttaca attgctgaag
    11341 ctcccacaag aaaccgtgtg gacattaaaa tttcggatac aaaaacagac gcagagaaga
    11401 agctcataga aaagcgtgtg aaagcagatt ttccgttaca tcttaatgtg ataatttctt
    11461 atgtaaatgc ataaaatcct gattaaaatt ttaatcaagc tatgttaata aattttgcag
    11521 ctttgcaaaa ccattcaaat aattaaataa ccatgaccaa aaataaaaat gacaagttga
    11581 aaaatctcag cttgtcattt ttctgtgtga atataaatcc ttataaaaag cactctgcaa
    11641 acgcttaggc ttaattatat ttttgctatt attatacctc atcgggtata aatgatgaat
    11701 tcagtgggta aaacaatccc tatcgggtat aatttatctg ttaagataat ataatattga
    11761 aattatacct gatagggtgt ataatgatga tatgaataaa attgctgaat ttattaaaca
    11821 aaaaagaaaa gaagcaggac tcactcaaga ggactttgca atcagaagcg gactgggact
    11881 tcgttttgtt cgtgaacttg aacaaggcaa ggaaacagtt cgtatggaca aggtaaatca
    11941 agctctctca atgtttggta tggaggctgt tcccggaaag aaggatgact gatggaagca
    12001 tacagaacag cgtatgtata tgtgaggaac aactttgccg gagttttaaa ggaaaaggat
    12061 tttggctatt tgtttgtata tgacagtg
//
LOCUS       DAOMVE010000043        12049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1219_length_12049_cov_4.498416, whole genome shotgun sequence.
ACCESSION   DAOMVE010000043 DAOMVE010000000
VERSION     DAOMVE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12049
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1219_length_12049_cov_4.498416"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /gene="aspS"
                     /locus_tag="PLS64_03835"
     CDS             complement(<1..1178)
                     /gene="aspS"
                     /locus_tag="PLS64_03835"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRL42994.1"
                     /translation="MAEFMTGMKRTDYCGDLRIGDVGREVTVCGWVQRCRDLGQLIFI
                     DLRDRTGIVQLAFNDKTDKEIFDKAFSCRSEFVLAAKGVVCERSSKNSEIPTGDIEVV
                     VSDMRVLSKAQTPPFEIVDDTNTNEELRLKYRYLDLRRRPLLNNLMMRSKIAKVARDY
                     FHDNGFVEIETPMMIKSTPEGARDYLVPSRIHNGKFYALPQSPQIYKQLLMLSGFDRY
                     IQLARCFRDEDLRADRQPEFTQIDMELSFVDVDEILEINEGFFKKLFKEVLNIDLEEH
                     FQRMTYKEAMESYGSDKPDIRFDMKIQDISDLVKDCGFSVFTSAIENGGSVRAIVAKN
                     ASSVYTRKEIDKLTEYAKGIGAKGLAYVRWVDEPNASFKKFMSEEELSAIYERLGAEK
                     G"
     gene            complement(1248..2540)
                     /gene="hisS"
                     /locus_tag="PLS64_03840"
     CDS             complement(1248..2540)
                     /gene="hisS"
                     /locus_tag="PLS64_03840"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HRL42995.1"
                     /translation="MALITKKIKGTEDVLPKESYKWQFVEKIMREEARSYGFREIRTP
                     VFEHTELFARGVGQTTDVVQKEMYTFDTKGGESVTLRPEGTAGAARAVLEHALENEGL
                     PIKASYFVSCYRYEKPQAGRLREFHQFGIEEYGTQDPVADAELISLASSVINRLQLDS
                     VHLQINSIGCPTCRAEYHKALKSYFEGYKDKLCDTCLSRLDKNPMRILDCKSPICSEI
                     AKDAPKITDYLCDECRNHFDAVQKYLDAAGIEYTVDPTIVRGLDYYTKTVFEFVTDCI
                     GAQGTVCGGGRYDGLIEELGGKHLPSLGFAMGMERLLMVMDKQGIEIPDNDECMLYIA
                     TMGDAAKVKAFELIKQVRSCGMIAETDVIGRGLRAQMKYADKIDARYSIVLGDNEIEQ
                     NTAKVKNMETGETAEVTLDGHFMEKFIEIQLSDEQKNK"
     gene            complement(2657..3052)
                     /gene="mgsA"
                     /locus_tag="PLS64_03845"
     CDS             complement(2657..3052)
                     /gene="mgsA"
                     /locus_tag="PLS64_03845"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HRL42996.1"
                     /translation="MNIALIAHDEKKELMVQFCIAYCGVLSRNNLCATGTTGKMVSEA
                     TGLTIQKFLAGSQGGSQQIAARIACNEIDMLLFFRDPLNPKPNEPNDMNMLRLCDMHN
                     IPVATNIATAEVLIHGLERGDLDWRNILK"
     gene            complement(3125..3865)
                     /locus_tag="PLS64_03850"
     CDS             complement(3125..3865)
                     /locus_tag="PLS64_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HRL42997.1"
                     /translation="MDNVIVVASGKGGTGKSTVCICLSVALVKQGKRVLLIDCDCGMR
                     GLDIMLDMEQDIIFDASDAVCGNCTFGEAVYKSKNNENLYLMAAPFDTENELSPSVFT
                     QLVNSVKDSFDFVLIDSPAGIGSGFETAAAPADRALIVTNAEPTGVRGAVKVRRKLES
                     MGKTNIRLVINRFDRKLFTQLGFYEDLDSVIDATQTQLIALVPFDIRISVIVQRGVAG
                     LNWSAAASVFDCLAQRLEGKHIPLAFKG"
     gene            complement(4136..6157)
                     /locus_tag="PLS64_03855"
     CDS             complement(4136..6157)
                     /locus_tag="PLS64_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRL42998.1"
                     /translation="MRFGKSRKNGKKSKKSRTTVCIIITAAIFAGFAIRLVDWQLVQG
                     KSYKSLAAKSTGYTEKTDATRGEIVDRNGVGLVVNTTKYKIVLNKLYIEEDRLDGILL
                     ELADILTKTGDARTDSLPISVGSDGSCVYKTSREEDAEKLLSSDFLDMDRNTSAGDCF
                     DALLKRYKISDRLSLSQKTTLVSIHYNMELEKYSNSNPYVFAKNISRSAVNAVSENTQ
                     GVSGVEIQTYLTRGASDPDLAPHILGALGSLSEDEYNELKKDGKDYSYNDKIGKFGIE
                     LACEDDLKGKGGTRVIQRNADGTLVESIETESAKPGNTVYLTIDSKLQKTAVKSLEKN
                     VKAAKQAGKESGQKNNGEDCETGAVVMLSVKDFSVLAAASYPTYDLNRYSEYGSYYVK
                     LSENKNSPMYNRATVGTFACGSVFKPCVAGAALEEKIITDKTKIYCKQDYDFYPTNVV
                     RCMHYHGSLDVTGAIEQSCNYFFADTGRRLGIEPMYLYAQKFGLGEYTGVEIEESKGT
                     LAGRDSTDWQIGNTVSAAIGQSDNAFTPVQLATYVATIANNGERLKTHIVDKVTNYER
                     TKTVKSTDVESYGDCGISKKNLDIVRKAMLGVTQDENGTANYMFGDYKVKVAAKTGTA
                     ENSGSDHTTFICYAPYEKPEVAIAVVIEHGAKGRYSMQVAKDLLDAYFN"
     gene            complement(6181..6684)
                     /locus_tag="PLS64_03860"
     CDS             complement(6181..6684)
                     /locus_tag="PLS64_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL42999.1"
                     /translation="MMRASRTFFRFFGYSLLLIILYVFQGSGFIIPEIFGGRPLMLIS
                     LALCIASFENTLPSVIFGALCGVLTDISFDGSIGYFAFALTIVCYAENFVFSKYFVPS
                     FVTVIVFSFAAVMTVMLIYYLLFVLIAGLDSGCVLFVRHYISRILYTFAVTIPLYFLT
                     AFLNKNL"
     gene            complement(6681..7502)
                     /gene="mreC"
                     /locus_tag="PLS64_03865"
     CDS             complement(6681..7502)
                     /gene="mreC"
                     /locus_tag="PLS64_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRL43000.1"
                     /translation="MRKFLHNKNSKILIITLVILTVMSVFSHNGSNIFSGAVNTVTKG
                     LSTLTAGAASSDKSKEELEKENEQLKKENAELREQLVDYLDAKEENAKLWKYYELKKE
                     NPSYDILPAGVLRRDANDDFYSFTLDKGSTDNVEKNDPVITENGLIGWVSDVELTTCR
                     VRTILSPDTKASAIDKKTSDNGIISGNAEYCDDNLTCLTKIAENNKIKVGDIVVTSGT
                     GGVYPKNLIIGKVKELKFNSYDTTRYAVVEPYEDIRTVTSAAIITDFDGKGEVKK"
     gene            complement(7577..8587)
                     /locus_tag="PLS64_03870"
     CDS             complement(7577..8587)
                     /locus_tag="PLS64_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRL43001.1"
                     /translation="MFSFSKDIGIDLGTANTLVCLKGKGIILREPSVVAVDMRTDEVL
                     AVGSQAKEMIGRTPGSIVAVRPLKDGVIADFKITAKMLQHFIKKAVKPGIFSKPRVII
                     CMPSGVTEVERKAVEDAAYQAGAKPPVVLIEEPMAAAIGAGMPVDEPCGSMVVDIGGG
                     TSEVAVISLGDIVTACSVRVAGDKFDEAISTYVKRKYNLLIGERTAEDIKMKIGSAYP
                     GEEEKNLVVKGRNLVDGLPKDITISSAEVREALADPLATIVEAIRTTLEKTPPELSAD
                     IIDHGIMLTGGGALLKGLDMLVMQQTGMPVHIAERPLDCVVDGAGKRLGKPLGVQYKH
                     DR"
     gene            complement(8698..9138)
                     /gene="dut"
                     /locus_tag="PLS64_03875"
     CDS             complement(8698..9138)
                     /gene="dut"
                     /locus_tag="PLS64_03875"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRL43002.1"
                     /translation="MTVKIKKVKDNAKIPKRATNGSAGMDLYACIDEKITINPGQLVL
                     VPTGIAIELPDNSVAAFLYARSGLGIKHGICLSNGVGVVDSDYRGEICAGLCNVSDKP
                     YEIEPFERICQMVIAPVVPCDVEEVTELGNTERGEGGFGSTGKK"
     gene            complement(9308..11371)
                     /locus_tag="PLS64_03880"
     CDS             complement(9308..11371)
                     /locus_tag="PLS64_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRL43003.1"
                     /translation="MKKIEILAPAGSFDSVIAAVRSGADAVYLGEKAFSARSSAKNFD
                     DEELKKATAYCHIHGVKVYVTINTIVFDDELEKLKKAIISAAEADVDALIVQNMGVAR
                     LAHRLVPDLALHASTQMSIHTASGVKALYEMGFKRVVLAREMSKKEIEKCCEIPVELE
                     VFVHGALCMCVSGQCYLSAMIGARSGNRGSCAQPCRLPFSVNNQKGGHALSLKDNSIV
                     NYLDELQNMGVASAKIEGRMKRPEYVSAAVRACVEQRDFGFISDKTQKMLRGVFSRTG
                     FTDAYYIGKTGSHMFGTRTKSDVVSADEKLFSAIRSSYKDEIGNVEITFDFTAKLGEN
                     PVLVASDGVHTVKKIADTVTEKAINRPIDAEKCRKQLEKTGSTAYNPTNVNINIDDDI
                     SIPLSIINSLRRDVLAELDTARSVVHNYKINRDYEITFPKFTPPVEKSTRARVPQTKL
                     SDAFKKCELVFVPLFADKRELVRLKNEGFNIGVEIPRGMFGREDTIAKKLSEMKEIGI
                     SDVLCNNLGALYIAKNLGFTLHSGFGMNFVNTLDLLWAEEYGIKDAELSFELDFKRIN
                     ALGGNIPRGIISYGYLPLMLCRSCPVKGAGIDCKTCKNHSKMKDRLGKQFLLKCDGNC
                     TEVLNCDLLFVPDKQNLTLLTSFNILRFSVENYVETVESLNESSLYPMLKDKLTYGLY
                     RRGVN"
     gene            complement(11368..12012)
                     /gene="zapA"
                     /locus_tag="PLS64_03885"
     CDS             complement(11368..12012)
                     /gene="zapA"
                     /locus_tag="PLS64_03885"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HRL43004.1"
                     /translation="MEKKRVSVQIEGRSYSLITTDDEKYVQKVANEVLSHIRKAAQST
                     KQLDTRDCAVLAALNFCDDRNKALKKNKDYVEKADKIIQQTNDLNRLCKEYREKLTEA
                     MNDNTRLINNNKELEKRLAVLGHEVSQLKEKLESVEKSKEKAKSEKSEPEKNSVPKEK
                     KTSNAKNNMTEQSDDIPVNVLSNEAKKTEQELRNEKLLGYVPMTQFSLFDEDKK"
BASE COUNT         3148 a         2833 c         2383 g         3685 t
ORIGIN      
        1 cccttttctg caccgagtct ttcgtaaatt gccgaaagct cttcttcact cataaacttc
       61 ttgaatgatg cgttaggctc gtcaacccat cttacataag caagtccctt tgcgccgatg
      121 ccctttgcat attctgtgag cttgtcaatt tcttttcttg tgtaaaccga cgacgcattc
      181 ttggcaacaa ttgctcttac agaaccgccg ttctcaattg cacttgtaaa tacgctgaaa
      241 ccgcagtcct taacaagatc cgaaatgtcc tgaatcttca tgtcaaaacg gatatcgggc
      301 ttgtccgaac cgtagctttc catagcctct ttgtaggtca ttctctggaa atgctcctca
      361 aggtcaatgt ttaaaacctc tttgaaaagc tttttgaaga agccttcgtt gatttcgaga
      421 atttcatcaa catcaacaaa tgaaagctcc atatcaatct gtgtgaattc aggctgacgg
      481 tctgcacgaa gatcctcgtc acggaaacat cttgcaagct gaatgtaacg gtcaaagccc
      541 gaaagcataa ggagctgttt gtaaatctgc ggtgactgag gaagagcata aaacttgccg
      601 ttgtggattc ttgacggaac aaggtaatct cttgcgccct cgggagttga cttgatcatc
      661 ataggagttt caatttcaac aaagccgttg tcgtgaaaat aatctcttgc aacctttgca
      721 atcttacttc tcatcataag attgttgaga agaggacggc ggcgaaggtc aagataacga
      781 tatttaagtc ttaactcctc gtttgtgttt gtatcgtcaa caatttcaaa aggcggcgtc
      841 tgtgcctttg aaagcacacg catatcgctt acaacaacct cgatatcgcc tgtggggatt
      901 tcgctgtttt ttgaacttct ttcgcaaaca acaccctttg cggcaagaac aaattcactt
      961 ctgcatgaaa atgccttatc aaaaatttct ttgtctgttt tgtcattgaa tgcaagctga
     1021 acaatgcctg tgcggtcacg caagtcaatg aaaatgagct gaccgaggtc acggcaacgc
     1081 tgtacccaac cgcagacagt tacctctctg ccgacatcac cgatacgcag atctccgcaa
     1141 taatcggtac gcttcattcc tgtcataaat tctgccattg tatatgctcc tttatatgaa
     1201 tcggtcggca tttttaatgt gcctgaccga ccgaaaattt tcttttatta cttatttttc
     1261 tgttcgtccg aaagctgaat ttcgatgaac ttctccatga aatgcccgtc aagagtaacc
     1321 tcggctgttt cgcctgtttc catattctta accttggctg tgttctgctc gatttcgttg
     1381 tcgcccaaaa cgattgaata tcttgcgtca atcttgtcgg catacttcat ctgagcacga
     1441 agtcctctgc cgataacatc ggtttcggca atcattccgc agctgcgaac ctgtttgata
     1501 agctcaaaag ccttaacctt tgcggcatcg cccattgttg caatgtagag catacactcg
     1561 tcattgtcgg gaatctcaat gccctgctta tccattacca tgagaagtct ttccataccc
     1621 attgcgaaac cgagggaggg aaggtgctta ccgccgagct cctcgataag accgtcataa
     1681 cgaccgccgc cgcaaacagt accctgagca ccgatacaat cggttacaaa ctcaaatact
     1741 gtctttgtat agtaatcaag acctcttaca attgtgggat caaccgtgta ttcaatgcct
     1801 gcggcatcaa gatatttctg aactgcgtca aagtggtttc tgcactcgtc acaaagatag
     1861 tctgtaatct taggtgcgtc ctttgcaatc tcggaacaga taggactttt gcagtcgaga
     1921 attctcatcg ggtttttgtc aagacgggaa agacaggtgt cacacagctt gtccttataa
     1981 ccctcaaaat atgatttcaa agccttgtga tattccgcac ggcaggtcgg acagccgata
     2041 gaattaatct gaagatgaac gctgtcaagc tgaagtctgt tgattacgga gcttgcaagg
     2101 ctgataagct ctgcatcggc aacaggatcc tgtgtgccgt actcctcaat accgaactgg
     2161 tggaactcac gaagtctgcc tgcctgtggc ttttcatatc ggtagcatga aacaaagtag
     2221 cttgccttaa tcggaagtcc ttcgttttca agagcgtgtt caagaacggc tcttgccgca
     2281 ccggctgtac cctcggggcg aagtgttacg ctttcgccgc cctttgtgtc gaatgtgtac
     2341 atttctttct gaacaacatc ggttgtctga ccaacgccac gggcaaaaag ctctgtgtgt
     2401 tcaaacacgg gagttctgat ttctctgaaa ccgtaggagc gagcctcctc acgcataatc
     2461 ttttcaacaa actgccattt gtagctttcc ttcggaagaa catcttccgt acccttgatt
     2521 ttcttggtta taagtgccat tttttcctcc gaaaattaca atcttttata gaaaagggaa
     2581 acgaaaaaat ttccctaaaa aacacaacaa tcgggcagta ttacaccacc cgatatgtaa
     2641 agcgaaaata attaaattat ttgagaatat ttctccagtc aaggtcgcct ctttcaagac
     2701 cgtggataag cacctcggca gttgcaatgt ttgttgcaac cggaatgttg tgcatatcgc
     2761 aaagacggag catattcata tcgttaggct cgttcggctt tggattgagc ggatctctga
     2821 aaaagagaag catatcaatc tcattgcacg caattcttgc cgcaatctgc tgacttccgc
     2881 cctgagagcc tgcaaggaat ttctgaattg taagacctgt tgcctctgac accattttgc
     2941 ctgttgtgcc ggtggcgcaa aggttgttgc ggctgagaac accgcagtaa gcaatgcaga
     3001 actgcaccat cagttccttt ttttcatcat gagctataag ggctatattc atatatattt
     3061 cctccagaca tttatttcat atcaaattag tttcaaaact ttaactcaaa ataccccgca
     3121 caaactaccc tttgaacgca aggggaatat gttttccctc aagcctctgt gcaagacagt
     3181 caaaaacgga cgccgccgca ctccagttaa gtccggcaac acctcgctga acaatcaccg
     3241 aaatacggat gtcaaacggc acaagcgcaa taagctgtgt ctgtgttgcg tcaataacgc
     3301 tgtcgaggtc ttcataaaaa ccgagctgtg taaaaagctt tcggtcaaag cggttgataa
     3361 caagtcggat atttgttttt cccattgact caagctttct tctcacctta accgcaccac
     3421 gcacacccgt cggctctgca ttggtaacaa tcaaggctct gtcggcagga gccgccgcag
     3481 tttcaaagcc cgaaccgatt ccggcagggg agtcgataag gacaaaatca aagctgtcct
     3541 ttactgagtt gacaagttga gtaaacaccg agggactgag ttcgttttcg gtgtcaaagg
     3601 gagccgccat aaggtagagg ttctcattgt ttttgctttt gtaaacagcc tcaccgaatg
     3661 tacagttgcc gcatacggca tccgacgcat caaaaattat gtcctgctcc atatcgagca
     3721 taatgtcaag accacgcatt ccgcagtcac aatcaataag caagactctt ttgccctgct
     3781 ttacaagagc aacggaaaga caaatgcaga cggttgattt acccgttccg ccttttcccg
     3841 acgctacaac tataacatta tccataataa attctacctc tgctatattc tatcacaaaa
     3901 tccgttaaag agcaagaaac gaacattatt tttatcagcc aaaatttttg ttgatatttt
     3961 agtgatagtt aataaaatta ttcttcattt ttaacggttt gcacaataat ttcattgatt
     4021 tttaatgcta aaagcaaaaa ataagttttt tatgtttggt cgttcagcca ttatacggtc
     4081 gggatttttg ttttatcttt taacatatcg tattctcgac cataattttc aattttcaat
     4141 taaaatatgc gtccagcaaa tcctttgcaa cctgcattga atatctgccc tttgcaccgt
     4201 gttcgattac aacggcaatt gcgacctcgg gcttttcata cggagcatag caaatgaatg
     4261 ttgtatggtc tgagcccgag ttttcagccg tacctgtttt ggcggcaacc ttaactttat
     4321 aatcaccaaa catataattt gccgtgccgt tttcgtcctg agtaactccg agcatcgcct
     4381 tgcgaacaat atcaagattc ttctttgaaa ttccgcaatc accgtagctt tcaacatctg
     4441 tcgattttac ggtttttgtc cgctcataat ttgtaacttt atcaacaatg tgtgttttga
     4501 gcctctcacc gttgttggcg attgtcgcaa cataggttgc aagctgaacg ggagtaaatg
     4561 cgttgtcgga ctgaccgatt gcggctgaaa ctgtgttgcc gatctgccaa tctgtgctgt
     4621 ctctgcctgc aagagttccc tttgattcct caatttcaac acccgtgtat tcaccgagac
     4681 cgaacttctg cgcatataga tacatcggtt caattccgag ccttctgccc gtgtcggcaa
     4741 aaaagtagtt gcacgattgt tcaattgcgc ccgtaacatc aagactgccg tgatagtgca
     4801 tacaccttac cacattggta gggtaaaaat catagtcctg tttgcagtag attttcgttt
     4861 tatcggttat gattttctcc tcaagagccg cacccgcaac acacggcttg aacaccgaac
     4921 cgcaggcgaa tgttccgact gttgcacggt tgtacatcgg agaatttttg ttttcggaaa
     4981 gtttgacata gtacgagccg tattcgctgt acctgttcaa atcgtatgta ggatagcttg
     5041 ccgccgcaag taccgaaaaa tccttgaccg acagcattac aactgcacct gtttcacagt
     5101 cctcaccgtt atttttctgt cccgattcct tgcctgcctg ctttgccgct ttgacatttt
     5161 tttcaagcga ttttacagcg gttttctgca attttgaatc aattgtaagg tagaccgtgt
     5221 tacccggttt tgcactctcg gtttcaatgc tttcaacaag agttccgtcg gcatttcgct
     5281 gaataaccct tgttccgccc ttgcctttga ggtcatcttc acaggcaagt tcaattccga
     5341 atttgcctat cttatcatta taggagtaat ccttgccatc ttttttcagc tcattgtatt
     5401 catcttccga caacgaaccg agtgcgccga ggatgtgcgg tgcaaggtcg gggtcagaag
     5461 cacctcgtgt gagataggtc tgaatttcaa caccgctcac gccctgagta ttttcgctga
     5521 cggcattcac ggcacttcgg ctgatatttt tggcaaaaac atacggattt gaatttgaat
     5581 acttttcaag ttccatattg taatgaattg aaacgagcgt tgttttttga cttagactta
     5641 acctgtcgga aattttgtag cgttttaaaa gtgcgtcaaa gcaatcaccg gcacttgtgt
     5701 tcctgtccat atcaagaaaa tccgatgaca gaagtttttc cgcatcttct tcacggcttg
     5761 ttttataaac gcaactgccg tccgagccga ccgaaatcgg caatgaatct gttcttgcat
     5821 ctcctgtttt tgtgagaata tcagcaagct caagtaaaat tccgtcaagc ctgtcctcct
     5881 caatataaag cttattgagg actattttat actttgtggt gtttacaaca agacctacac
     5941 cgttgcggtc aacgatttcg ccccttgttg cgtcggtttt ttcggtatat cccgttgatt
     6001 ttgcggcaag actcttgtag ctttttccct gtacaagctg ccaatcgaca agcctgattg
     6061 caaagcctgc aaaaattgcg gctgttatta ttatgcagac tgttgttctg ctctttttac
     6121 tttttttgcc gttttttctg cttttgccga atctcatacc gaacctccct ttcacaaaga
     6181 ttaaagattt ttgtttaaaa atgccgtgag aaaatacagc ggaattgtca cggcgaatgt
     6241 gtaaagtatt cttgaaatgt agtgccgtac aaacaacacg caaccgctgt caagccctgc
     6301 aatcaagacg aacagcagat aataaatcaa cattaccgtc atcaccgccg caaatgaaaa
     6361 cacgataacg gtaacaaatg acggtacaaa atatttgctg aatacaaagt tttccgcata
     6421 gcagacaatc gtaagtgcaa aggcaaaata gccgatactg ccgtcaaatg aaatatccgt
     6481 cagcacaccg cacaacgcac cgaaaattac cgacggcaaa gtgttttcaa aagaagcaat
     6541 gcagagtgca agtgaaatca acataagcgg tctgccgccg aaaatttcgg gaattataaa
     6601 acccgaaccc tgaaaaacat acagaattat taacagcagg gaatagccga aaaatcgaaa
     6661 aaaagttctt gaagctctca tcatttttta acctcgccct tgccgtcaaa atcggtaata
     6721 attgcggcag aggtcacggt gcggatgtcc tcatacggct caacaacagc ataacgggtt
     6781 gtgtcatagg aattgaactt cagttcttta accttgccga ttatgagatt tttcggataa
     6841 actccgcctg ttcccgaggt tacaacaatg tcgcctactt tgattttgtt gttctctgca
     6901 atctttgtca gacaggtgag attatcgtca caatattcag cgtttccgct tatgattccg
     6961 ttgtcggaag tctttttgtc aattgcagac gctttggtgt cgggcgaaag aattgttctt
     7021 acccgacagg ttgtgagttc aacatcactc acccagccga taagaccgtt ttccgtgatt
     7081 acgggatcat ttttttccac attgtctgtt gaacccttgt caagagtgaa cgagtagaaa
     7141 tcatcgttcg catcccttct cagcacacct gccggaagaa tgtcgtatga cggattttcc
     7201 tttttcagct cataatattt ccacagcttt gcattttctt ccttggcgtc aaggtagtca
     7261 acaagctgtt cacggagttc ggcattttcc tttttaagct gttcgttttc tttttcaagt
     7321 tcttcctttg acttgtcgga agacgccgca cctgcggtca gcgttgaaag tccttttgta
     7381 accgtgttta ccgcacccga aaaaatgtta ctgccgttat gagagaaaac actcatcaca
     7441 gttagtatga cgagtgtaat tatcaaaatt tttgagtttt tattatgtaa aaattttctc
     7501 atatctcata tttatattac aaatacggtc gggcggtgtt ttttcaatct gcccgacctt
     7561 actttgtcga tttttattat ctgtcgtgct tgtactgaac accgagcggt ttgccgagtc
     7621 ttttgcctgc accgtcaaca acacagtcaa gcggtctttc ggcaatgtga acaggcatac
     7681 ccgtctgttg cataacaagc atatcaagtc ccttgagtaa cgcaccgccg cccgtgagca
     7741 taataccgtg gtcgattatg tcggcagaaa gctcaggcgg agttttttca agagtggttc
     7801 tgattgcctc aacaattgtt gcaagcggat cggcaagagc ctctctgacc tcggctgagg
     7861 aaattgtaat atcctttgga agtccgtcaa caaggtttct gcccttgaca acaaggtttt
     7921 tttcttcctc acccggatat gcggagccga ttttcatttt gatgtcttcg gcagttcttt
     7981 caccgatgag aagattatat tttcttttga cataggttga aatcgcctcg tcaaacttat
     8041 ctccggcaac tcttacggaa caggctgtaa caatgtcacc gagagaaatt acggcaacct
     8101 cgcttgtgcc tccgccgatg tcaacaacca tacttccgca cggttcgtca acgggcatac
     8161 ctgcgccgat tgcggcagcc attggttctt cgataagaac aacgggaggc tttgcacctg
     8221 cctgatatgc ggcgtcctca acagcctttc tctcaacctc ggtaacaccc gaaggcatac
     8281 agattataac tctcggcttg ctgaaaattc cgggtttcac cgcctttttg ataaagtgct
     8341 gaagcatttt tgcggttatt ttaaaatcgg caataacacc gtccttgagc ggacgaaccg
     8401 caactatgga accgggagtt ctgccaatca tttcttttgc ctgtgagccg actgcaagaa
     8461 cctcgtcggt acgcatatca actgcgacaa cgctcggttc acgcaaaatt attccctttc
     8521 ctttaagaca aacaagagtg tttgcagttc caaggtcaat tcctatatcc tttgaaaacg
     8581 aaaacatccc acagcctcct tttacgctgt aatacccaaa tgtattttac tcaggcagta
     8641 tacttttatc atatatcttt tcacaaataa gggcaatgat aaattgtcga aattctttta
     8701 ttttttaccc gttgagccaa aaccgccctc gccacgctct gtgttgccaa gctccgtaac
     8761 ctcttcaaca tcacacggaa caacaggagc gatgaccatc tgacagattc tttcaaacgg
     8821 ctcaatctca taaggcttgt ccgaaacatt gcaaagacct gcgcagattt ctccacggta
     8881 gtcgctgtca acaacaccca caccgtttga aaggcagatg ccgtgcttga ttcccagacc
     8941 gcttcttgca tagagaaatg cggcaacgct gttgtcggga agttcaattg ctatacctgt
     9001 cggaacaagc acaagctgac ccggattgat tgtgattttt tcgtcaatgc aagcatataa
     9061 atccataccg gcagaaccgt ttgtggctct tttcgggatt tttgcattgt cttttacctt
     9121 ttttatttta acagtcatag tttaccatcc ttcatttcct gcaaaaattt gcaggaatta
     9181 ttcattactg tttttatttt tctctaaaaa aattatttat tgcaaaaatg taattataaa
     9241 tctctgttaa acggaaaaat cactttttcc acaaaacaat aatgattatt ttaacatttt
     9301 caacaatcta attaacacct cggcggtaca gtccatatgt taatttatct ttcaacatcg
     9361 gataaaggct tgattcgtta agactttcca cggtttccac atagttttca acactaaatc
     9421 ttaaaatgtt gaaagatgtt aaaagtgtta aattttgttt atcaggaacg aaaagcaagt
     9481 cacaattcag aacctcggta cagtttccgt cgcatttcag taaaaattgt ttaccgagtc
     9541 ggtctttcat ttttgaatga tttttgcaag ttttgcagtc tatacctgca cccttgacag
     9601 ggcaactcct gcaaagcata agaggaaggt agccgtagct gataattccc ctcgggatgt
     9661 tgcctccgag tgcgttaatt cgcttaaaat cgagttcaaa actgagttct gcgtccttga
     9721 ttccgtattc ctccgcccac aaaaggtcga gagtatttac aaaattcatt ccgaaacccg
     9781 aatgaagtgt gaaaccgaga tttttggcaa tgtataatgc tccgagattg ttgcacaaca
     9841 catcggatat gccgatttct ttcatctcgg aaagcttttt tgcaatcgta tcttcccgtc
     9901 cgaacattcc tctcggaatt tcaacaccga tgttaaagcc ctcgtttttg agccttacaa
     9961 gctccctttt gtcggcaaaa agcggaacaa aaacaagctc gcatttttta aaagcatcgg
    10021 aaagctttgt ctgaggcact cttgcacggg tggatttttc cacaggcggt gtgaatttag
    10081 gaaaagttat ttcgtaatca cggttgattt tgtagttgtg aacaacactt ctcgccgtgt
    10141 cgagttcggc aagaacatct ctgcgcaaag aattgataat tgaaagcgga atagaaatgt
    10201 catcatcaat atttatgttc acattggtgg gattatatgc cgttgagcct gttttttcaa
    10261 gctgttttct gcacttttcc gcatcaatcg gtctgttgat tgctttttcg gttactgtat
    10321 ctgcaatctt tttgacggtg tgaactccgt cagaggcgac taaaacggga ttttcgccga
    10381 gttttgccgt aaaatcaaaa gttatttcaa catttccgat ttcgtccttg taggaagaac
    10441 ggattgccga aaacagcttt tcatcggcag aaacaacatc cgattttgtt cttgtgccga
    10501 acatatgact tcccgttttg ccgatgtaat aagcgtctgt aaagcctgtt cgtgaaaaaa
    10561 cccctctgag catcttctga gttttgtcgc tgataaagcc gaaatcacgc tgttcaacac
    10621 acgctcttac tgcggctgaa acatactcgg ggcgtttcat tctgccctca attttagccg
    10681 aggcaactcc catattttga agttcatcaa ggtaatttac aatactgttg tctttaagac
    10741 ttaaagcgtg accgcctttt tgattgttta cggaaaacgg cagtcggcac ggctgggcac
    10801 aggaaccacg gtttccgctc cttgcgccaa tcattgcgct gaggtagcat tgtcccgaaa
    10861 cgcacataca caatgcaccg tgaacaaaaa cttcgagttc aaccggaatt tcacagcatt
    10921 tttcaatctc ttttttgctc atttcacggg caagaacaac acgcttgaaa cccatttcat
    10981 aaagtgcctt tacaccggat gcggtgtgaa tgctcatctg ggttgacgca tgaagtgcaa
    11041 ggtcgggaac aagcctgtgt gcaagccttg ctacacccat attctgaaca atgagtgcgt
    11101 caacatcagc ctctgcggct gaaattatgg cttttttcag cttttcaagc tcgtcatcga
    11161 acacaatcgt attgattgtg acatacacct ttacaccgtg aatgtgacag tatgccgttg
    11221 ctttctttag ttcttcatca tcaaaatttt tggcagatga acgggcggaa aatgcctttt
    11281 cgccaagata cactgcgtct gctccgctcc ttacggcggc aattacactg tcaaagcttc
    11341 ccgccggagc aagtatttct atctttttca tttcttatct tcatcaaaca gagagaactg
    11401 cgtcattgga acataaccga gaagtttttc attacgcaat tcctgttctg tttttttagc
    11461 ctcgtttgac agaacattca cgggtatatc atctgactgc tctgtcatgt tattttttgc
    11521 atttgaagtc tttttttcct ttggaactga atttttttca ggctctgatt tttcagattt
    11581 ggctttttct ttgcttttct caacactttc gagcttttct ttaagctgtg aaacctcgtg
    11641 tccgagaaca gcgagtcttt tttcaagctc tttattatta tttataagtc gagtgttatc
    11701 gttcatggcc tccgtaagct tttctctgta ttccttgcaa agcctgttaa gatcatttgt
    11761 ctgctgaata attttgtcgg ctttttcaac ataatctttg ttctttttaa gagccttgtt
    11821 gcggtcatcg cagaagttaa gtgccgcaag aaccgcacaa tcacgggtat caagctgctt
    11881 tgtgctttgg gcggcctttc ttatgtgaga aagcacctcg tttgctacct tttgaacata
    11941 cttttcatca tctgttgtga taagtgaata acttcttccc tcaatctgaa cgcttactct
    12001 ctttttctcc ataggaaatg catctccctt ttatagcttt ttcgtcctc
//
LOCUS       DAOMVE010000044        12003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1228_length_12003_cov_2.739036, whole genome shotgun sequence.
ACCESSION   DAOMVE010000044 DAOMVE010000000
VERSION     DAOMVE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 12003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12003
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1228_length_12003_cov_2.739036"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLS64_03890"
     CDS             <1..640
                     /locus_tag="PLS64_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRL43005.1"
                     /translation="SANSGNRLSAATFEFTYDPDYIEFRKVKAVNNGSKIQVNKKSGK
                     VKVVFLNSEGQNISGGSDVLTLDFKTIKEGTAYIDYTVNQCVDSDVNFMDVGSCTASC
                     IRIYKNASGTVSGGSSSSSNKNSTQATGKGGKSKHPTEPTTVNASDDEYIDYNPIKDK
                     QIKFFLAGMGITAGCLAILAVAYAIGKKSAQKKAEKEKNHNDNTLENKTSDD"
     gene            complement(653..1555)
                     /locus_tag="PLS64_03895"
     CDS             complement(653..1555)
                     /locus_tag="PLS64_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523893.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HRL43006.1"
                     /translation="MNYYSLNKYLKNTFGEKVYKISLNGNMTCPNRDGTLGTRGCIFC
                     SRGGSGEFASDALLPVHGQIDQAKLRIKKKSDCKKFIAYFQPFTNTYAPVEYLEKIFT
                     DAISEPDIVALSIATRPDCLGNNVLGLLEKLNKIKPVWVELGLQTIHEKSADYIRRMY
                     PLSVYDSATENLHKIGINVITHIILGLPNESKEMMLESVKYAGSKTDGIKLQLLHVLK
                     NTDLCDDYESGKFDVLSMEDYIDILCDCVEVLPENVVIHRLTGDGDKKLLVAPMWSAD
                     KKRVLNSINREFAKRDITQGRKAK"
     gene            complement(1771..2454)
                     /locus_tag="PLS64_03900"
     CDS             complement(1771..2454)
                     /locus_tag="PLS64_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43007.1"
                     /translation="MFEFTIRENDFLNGFLNLLSDYVNKSYKFTMSYLTSPLLSDVRE
                     SERLVLLVVNKSVFQNMYSFIKLNDSNMQFASFSCLETAVNSMRLYNVLATNPDYMHE
                     YITGGNFSLEVCENEISEKQKKYKKGEEEFSLREFATGLGKFNSFELKNAAVASQLVD
                     GNIFLGLSCGNELGEDLQDEVRKSMIGAYLSLSKHNKMFFNGGIDDTLEELEDKLYAK
                     FLEYIRKFS"
     gene            complement(2463..3122)
                     /locus_tag="PLS64_03905"
     CDS             complement(2463..3122)
                     /locus_tag="PLS64_03905"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HRL43008.1"
                     /translation="MKSVRIWFKKDFECRYISHLDLNRCMLRALHKSKIPIWHTEGFN
                     PHPFATFPLPLSLGFRGVNECMDVKLEDESFPFEEIISRLNGCLPRGLRVFDVTEPVM
                     KAGKIAFASFTIKIACDGENSRTVCEQLKELLTSESIEVEKKSKKGIKTVDIKQGIKS
                     FAVTDKFDFTELDVVLSAGSSDNVNPNLLVSAFENRFLVSCDIDITRNDLYNADMELF
                     R"
     gene            complement(3097..4953)
                     /locus_tag="PLS64_03910"
     CDS             complement(3097..4953)
                     /locus_tag="PLS64_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HRL43009.1"
                     /translation="MVSKEVEKLLLKVQKPGRYVGGELNEVIKDKKKVDCRFAFCFPD
                     TYEVGMSHLGMKILYSLMNAVPYIWCERVFAPWVDMEEEMIKHNIPLYALESGDPVSD
                     FDFIGFTLQYELSFTNMLNMLRLSGVPIKSCDRKELKNIVVAGGPCACNPEPIADFVD
                     IFFIGEGEEVDLEVIELFRKCRAEGKSKQEFLELSSKIEGVYVPALYDVTYNEDGTIK
                     SFTPKGDAPAKIHKRIMLDMDKSFYPDNFVVPLVEIVHDRAVQEIFRGCIRGCRFCQA
                     GMIYRPVREKSPETINKQCRALCDNTGYDEVSLSSLSSSDYSQIVPLLDKLNEWSKDE
                     KVSISLPSLRVDGFTDDIMNKIKTVRKSGLTFAPEAGSQRMRDVINKNVREDELLQTC
                     TTAFAGGWTTVKLYFMIGLPTETMDDVAAIAGLGQSVVDAYYKCPDKPKGKSVRVTVS
                     ASSFVPKPFTPFQWEPQDTIPVLHEKQQHIKDSITTRKIQFNYHDADTSYLEAVFARG
                     DRKLCKVMELACERGFHFDGWNDCFSLEKWLELFDECGVDPSFYANRHRSFDEILPWD
                     HIDYGVTKDFLIRECKKAYADTTTPHCRLKCNNCGAAKYGKGVCFEKRKNMV"
     gene            complement(4966..5466)
                     /locus_tag="PLS64_03915"
     CDS             complement(4966..5466)
                     /locus_tag="PLS64_03915"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRL43010.1"
                     /translation="MAVIIHECGHLFAMLICKSPPDKIKISLFEIKITNSSRQLNTTR
                     QNIFIIFFGPLVNFICFILFYLLYLCNSEFLLPIAMANLCTGLFNMLPVMSLDGGQLM
                     YVILSRKFSEKSAERIINITAVIILFPLTVLGFMVLLNSKYNFSLLFVCTYLIASLLC
                     KNNRYY"
     gene            complement(5549..5929)
                     /locus_tag="PLS64_03920"
     CDS             complement(5549..5929)
                     /locus_tag="PLS64_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43011.1"
                     /translation="MTENNDSRNTITYDDDWKSVSYSEYPKVSDELDLDEEDVNSLSA
                     ENSDEKKNSPKQLVITIQLVCCIIIALAAFLLNLFGGEVYAATREWYYSNLEKSVVFD
                     TGDNKIDLSNLFTATSDEVSSAEH"
     gene            complement(5981..>7071)
                     /locus_tag="PLS64_03925"
     CDS             complement(5981..>7071)
                     /locus_tag="PLS64_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL43012.1"
                     /translation="DGATHGLVTCIFNYVIFLLLGLFAVRPFMAMYTQNEAIVNYGIQ
                     YLTVVLCFSFFSIVQIMVEKTLQATGNMIFPMLSQLFGAIVNIIFDPLLIFGIGIFPE
                     MGVLGAAIATVFGQFCGMVFCLCILFFKNNEVKVSFKHFKLNGSTLKSIYVVGFPSVI
                     MQSIGSVMIIGLNAILAVSEAAVTVLGIYYKLQSFVFMPCFGLNQGVMPIIGYNYGAR
                     KKKRMYSALKRGIIIGVIIMAVGTILMWTIPEQLIAMFGGTQDIMDIGVPAFRIISLC
                     FIPAAAGIIFTTLFQAVGKGLRSLIMSFCRQLVLILPIAWVLSMVFDYTAVWYAFPIA
                     EFFSLMLAIAFFVNLTKGDFKSLDQKIE"
     assembly_gap    7072..7108
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7109..7337)
                     /locus_tag="PLS64_03930"
     CDS             complement(<7109..7337)
                     /locus_tag="PLS64_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL43013.1"
                     /translation="MENSVKPHENKMGTMPMTRLILTMSLPAIFSMTIQAMYNVVDSI
                     FIGNYDADGLTATSLAYPLQMLLIAFAAGTAV"
     gene            complement(7378..7848)
                     /locus_tag="PLS64_03935"
     CDS             complement(7378..7848)
                     /locus_tag="PLS64_03935"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRL43014.1"
                     /translation="MKTNSCSMRFRIIMFVLTASVIAFAFIHSSMPSVESAQESESVL
                     DFVTVILKFFGIDPNLSDHIIRKLAHFTEYTVLGALLCSCAYSFDRIKPIKFVPYTVS
                     IGLFTCFVDETIQLGIEGRSGQVSDMWIDFFGVLLGTAVMLVAFWIYRKIRKIN"
     gene            8045..8521
                     /locus_tag="PLS64_03940"
     CDS             8045..8521
                     /locus_tag="PLS64_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HRL43015.1"
                     /translation="MWYLIVFFISMVPIVEIRGAVPVAVANDLNLFWTYVICILGNML
                     PVPIIYLFARKVLVWGSDKKFIGRFFSFCLEKGEKGGRKLEAKAGNGMFVALMLFVGI
                     PLPGTGAWTGTLAASILDLGFKKSVIAVMCGVVIAGIIMGIGSVLVANGINFIFAP"
     gene            8572..>8911
                     /locus_tag="PLS64_03945"
     CDS             8572..>8911
                     /locus_tag="PLS64_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL43016.1"
                     /translation="MEKKNIYDAIIIGGGASGLMCAITAKKHDRSKKIAIIEKNDRLG
                     KKLMSTGNGRCNLTNHCISPDKYVGSFKKQSKKIFERFSGDEMANIFKNLGLLSFYDN
                     EGRYYPISKHA"
     assembly_gap    8912..8921
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8922..9804
                     /locus_tag="PLS64_03950"
     CDS             <8922..9804
                     /locus_tag="PLS64_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRL43017.1"
                     /translation="DVLRLQVETLGIDVFTKQNVNSIKKVSNGFKISSDDSEKKYDFI
                     CNKLVIANGSKAAPKLGGNASAIDYLKNFGHKVVSFSPALCPVKVKSDVLKTLKGLRV
                     TGKAQLYGEHGRLIKEETGEIQFTENSLSGICVFNLSLFAKQNDKIVLDLLPDYSENE
                     LIKIIRQHISLFSDLPCEQLLTGIFQKRIGQAILKISRVDLSKICSKLSKDEVSGICK
                     TIKSMSFTVSGKEDFSHAQCALGGVLGKEIDENTMMSKIVKNLFICGEAIDLCGECGG
                     FNLHFAFAGGIIAGENL"
     gene            9801..11294
                     /locus_tag="PLS64_03955"
     CDS             9801..11294
                     /locus_tag="PLS64_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43018.1"
                     /translation="MIRVNNIHLSLDYDDKSVRKAVAQQLRIEEKSIKSCKIFRRSVD
                     ARKKDNIYFLTTVDAELNINEEKVCKKCGNAQIARSGAKPILIERGRDVDRRTADVNR
                     FWTSGKLDITSNVQFGEGGAGTFSDGKLNSGTKDIRQRKVLEEFVSHGAPDEILYNAK
                     PHIGTDMLKGTIKNIRNEIIELGGRVMFETKLVSMAFSDNKLKSITVETANGDEIIET
                     DNVILAIGHSARDTFEMLYDLKLPIEAKPFSVGARIEHLRQKVDKAQYGRFAGNKKLG
                     SANYKLSTHLDNGRGVYTFCMCPGGKVVNASSEENRLCTNGMSEFARNAENSNSALLV
                     GINPDDYESDHPLAGMYLQRKLESKAFVAGGENYNAPIQRVDDFLNNRKSTHLGDVKP
                     SIGPNYEFSNLNEILPEYVCTSMAQGIVKMGRMLHGFDDGDAVLTGVESRSSSPVRIM
                     RKTDTLESVLIEGLYPCGEGAGYAGGIISAAVDGIKCAEKIIEKSNK"
     gene            complement(11495..>12003)
                     /locus_tag="PLS64_03960"
     CDS             complement(11495..>12003)
                     /locus_tag="PLS64_03960"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HRL43019.1"
                     /translation="VCDSTEVQKYIANISTLSDEALIAADVDKDSVISVKDATMIQAY
                     IVKLIVEDSYCGTYTENVAPTQPTTNVTEPTTEPTSIQTKNYVYFNNTENWSTVNVYV
                     WSSKDSTYMSWPGKAMESIGNNTYRFELPNGAEYAIFNNGSAQTGDLKIPDVNMIYSN
                     GQSSKYSE"
BASE COUNT         3791 a         2352 c         2352 g         3461 t
ORIGIN      
        1 ttccgcaaat tccggcaatc gactttctgc cgcaacattt gaatttacat atgaccccga
       61 ttatatcgaa ttcagaaagg ttaaagcagt gaataacggc tcaaaaattc aggtcaacaa
      121 gaaaagcggc aaggttaaag ttgtttttct taattctgaa ggacaaaata tttcgggcgg
      181 aagcgatgtc ttaacgcttg atttcaaaac aataaaagaa ggtactgcgt atattgacta
      241 cacagtgaat caatgtgttg attccgatgt gaactttatg gatgttggaa gttgcactgc
      301 atcgtgtatc agaatatata aaaatgcatc cggaacggtt agcggcggaa gttcctcgtc
      361 aagcaataaa aacagtacgc aagctacagg aaagggcggt aaaagtaaac acccgaccga
      421 gcctacaact gttaatgcct cagatgacga atatattgat tacaatccca ttaaagataa
      481 gcaaataaaa tttttccttg ccggcatggg cataactgcg ggatgcttag ctatattggc
      541 tgttgcatat gcaatcggta aaaaatcagc tcagaagaaa gctgaaaaag agaaaaatca
      601 caacgacaat actcttgaaa acaagacttc cgatgattga ttcggaagtc ttttactttg
      661 cctttcttcc ctgcgtgata tcacgctttg caaattcacg gttgattgag tttaaaacac
      721 gctttttatc ggcactccac ataggtgcaa cgagcagttt tttatccccg tcacctgtta
      781 aacggtgaat tacaacattt tcaggcaaca cttcaacgca atcgcaaaga atatcaatat
      841 aatcttccat tgaaagcaca tcaaattttc cgctttcata gtcatcacac aagtctgtgt
      901 tttttaaaac atgcaacaac tgcagtttaa ttccgtcggt tttactgcct gcatatttta
      961 cagattcaag catcatttct ttagattcgt tcggaagtcc caaaataatg tgtgtaatta
     1021 cattgattcc gattttatga agattttctg tagctgagtc ataaacggat agcggataca
     1081 ttcttcttat ataatctgcc gatttttcat gaattgtctg caatccgagt tctacccaga
     1141 caggttttat cttgttcagt ttttcaagaa gacctaacac attatttccc aggcaatcgg
     1201 gacgggttgc gattgaaagg gcaacaatgt cgggttctga aattgcatct gtaaatattt
     1261 tttcaagata ttcaacaggc gcataggtat ttgtgaacgg ctgaaagtat gcaataaact
     1321 ttttacagtc ggatttcttt tttattcgca gtttagcctg atcgatttgt ccgtgaacag
     1381 gaagaagtgc atcggaggca aattctccgg agcctcccct gctgcaaaaa atacagcccc
     1441 ttgttccgag ggttccgtca cgattcggac aggtcatatt accattcagc gaaattttgt
     1501 acaccttttc accgaatgta tttttgagat acttattaag ggagtagtaa ttcataataa
     1561 aacacctgat aaattaaaaa ggtcggcaga aaagccgacc tttgtgtctt tcgacatatt
     1621 agagaggaaa tctatgaaaa gttcttgtaa gcattatcta tgtgaagtta aagtgttttt
     1681 tatttatctt taacttacaa gcatataata actgtcttat gtgacagaaa attgaacgct
     1741 tgtgtaaagt aatgtgaaag tttaaaaaga ttatgagaac tttctgatat attcaagaaa
     1801 ctttgcatag agcttgtcct caagctcttc aagagtatca tctataccgc cgttaaaaaa
     1861 cattttattg tgctttgaaa gtgaaaggta tgcaccaatc atactctttc tgacttcatc
     1921 ctgcaaatcc tctccgagtt cattgccgca ggaaagtccg agaaagatat tgccgtcaac
     1981 aagctgtgac gcaacagctg catttttcaa ttcaaaagaa ttgaatttgc caagtccggt
     2041 tgcaaattca cggagcgaga actcctcttc acctttcttg tatttttttt gtttttctga
     2101 aatttcattt tcacatactt caagcgaaaa gtttccgccg gtaatatatt cgtgcatata
     2161 atccggattt gtagcaagga cattgtaaag tcgcattgag ttcacggctg tttcaagaca
     2221 ggaaaatgat gcaaactgca tattgctgtc attcagcttg atgaaactgt acatattctg
     2281 aaaaacagat ttgttgacta caagcaaaac aagtctttcg ctctcacgca catctgacaa
     2341 aagagggctt gtcaggtacg acatagtgaa cttatacgac ttatttacat agtcactcaa
     2401 taaattcaaa aatccgttta aaaaatcatt ttctcgtatt gtaaattcaa acatatattc
     2461 acctatctaa acaattccat atctgcgttg tagaggtcat ttcttgtgat gtcgatatca
     2521 caggaaacca aaaaacggtt ttcaaaagcc gatacaagca gattcggatt cacattgtcg
     2581 gaactgcctg ccgacaaaac aacatcaagt tctgtaaagt cgaacttatc ggttactgca
     2641 aagctcttta taccctgttt tatgtcaacg gtttttatgc cttttttaga tttcttttca
     2701 acttcaatac tttcgcttgt cagcagttct ttgagttgtt cgcatactgt acgggagttt
     2761 tcaccgtcac aggcaatctt gattgtaaat gatgcaaagg caatctttcc ggctttcata
     2821 acgggttccg tcacatcaaa cacacgaagt cctctgggca gacaaccgtt aagccttgag
     2881 atgatttctt caaacggaaa gctttcatcc tcaagcttga catccataca ttcgtttaca
     2941 cctctgaaac caagcgaaag cggcaacgga aatgttgcaa acggatgtgg gttaaatcct
     3001 tctgtatgcc agatagggat tttgctcttg tgcaatgcac ggagcataca tcgattgagg
     3061 tcaaggtgcg aaatatagcg acattcaaaa tcttttttaa accatattct tacgcttttc
     3121 aaaacacaca ccctttccgt atttagccgc accgcagttg ttgcatttca gtctgcaatg
     3181 cggtgttgtt gtatctgcgt acgctttttt acattctctg ataaggaaat ccttagttac
     3241 accgtagtca atatgatccc acggaagaat ttcatcaaaa cttctgtgtc tgtttgcata
     3301 aaatgacgga tcaacaccgc attcatcaaa gagttcaagc cacttttcaa ggctgaaaca
     3361 atcattccag ccgtcaaaat gaaaacctct ttcacatgca agctccatta ccttacaaag
     3421 ttttctgtca ccccttgcaa atactgcttc aagatagctt gtatcggcat cgtgatagtt
     3481 aaactgaatt tttcttgttg taatactgtc cttgatgtgc tgttgctttt catgaagaac
     3541 agggattgta tcctgcggtt cccattgaaa cggtgtgaac ggttttggta caaaagagga
     3601 tgcagacacg gttacacgca ccgacttgcc ctttggcttg tcgggacatt tgtaatatgc
     3661 atcaactaca gactgaccga ggccggcaat tgcggcgaca tcgtccattg tttcggtagg
     3721 cagaccaatc ataaaataga gttttaccgt agtccagccg cctgcaaatg cagttgtgca
     3781 agtctgcaaa agttcatctt cacgaacatt tttattgata acatcacgca tacgctgact
     3841 gcctgcctcc ggagcaaagg tgagtccgct cttacgtacg gttttaatct tattcataat
     3901 gtcatctgta aaaccgtcaa cacgcagtga cggaagtgaa atgctgactt tttcgtcttt
     3961 gctccactca ttcagcttgt caagcaacgg tacaatctga ctgtagtcac ttgaactgag
     4021 tgaagatagc gaaacttcat cgtaacccgt gttatcacat aatgctctgc attgtttatt
     4081 tatagtttcg ggagattttt cacggacggg acggtaaatc atacctgcct gacagaaacg
     4141 acagcctctt atacagccac ggaaaatttc ctgaaccgct ctgtcatgaa ctatctcaac
     4201 gagcggaact acaaaattat caggatagaa cgacttgtcc atatcaagca taatacgctt
     4261 gtggatttta gcgggagcat ctccttttgg agtgaatgac ttgattgtgc cgtcctcgtt
     4321 atatgttaca tcatagagtg caggaacata tacaccctca attttagatg agagttcaag
     4381 aaactcctgt tttgatttac cctcggctct gcactttctg aaaagttcaa taacctcaag
     4441 gtcaacctct tcaccctcac cgatgaagaa aatatcgaca aaatctgcaa tcggctcggg
     4501 gttgcaggca cacggtccgc ctgcaactac tatgttttta agttcttttc tgtcacagct
     4561 tttaatcggc acacccgaaa gcctgagcat attgagcata tttgtaaagc tgagttcata
     4621 ctgaagagtg aaaccgataa agtcaaaatc ggacacggga tctccgcttt caagcgcata
     4681 aagcggaata ttgtgtttta tcatctcttc ttccatatca acccatgggg caaaaactct
     4741 ttcgcaccaa atgtacggca cagcgttcat aagactgtaa agaattttca ttccgagatg
     4801 ggacatacct acttcatagg tatcgggaaa gcagaaggca aatctgcaat ctactttctt
     4861 tttatccttg attacttcgt tgagttcacc gccgacatat cttcccggct tttgaacttt
     4921 cagaagtaat ttttcaactt ctttgctgac catagaaatc ctccgtcagt agtacctgtt
     4981 gtttttacat aaaagtgacg caatgagata cgtacacaca aacaacagcg aaaaattata
     5041 ttttgaattt agcaaaacca taaagccgag taccgtcagc ggaaaaagaa ttataacagc
     5101 agttatattt atgattcttt cggctgattt ttccgagaat tttctgctca gaataacata
     5161 cataagctgt ccgccgtcaa gcgacataac cggcagcata ttaaaaagtc ccgtgcaaag
     5221 gttcgccatg gcaatcggca ataaaaattc actattacat aagtatagca gataaaacag
     5281 aataaaacaa atgaaattga ctaaaggtcc gaaaaaaatt ataaaaatgt tctgacgggt
     5341 tgtgtttaac tgccttgaag agttggtaat cttgatttca aacagcgaaa ttttgatttt
     5401 atcaggcgga gatttgcaaa tgagcattgc aaaaagatga ccgcactcat gaataatcac
     5461 agccataaca caacatagaa atcctaaaaa tatattcagt atcacacata ttgctccaag
     5521 acaaacgagt gaatacgaaa ccgtaaaatc aatgttcagc agacgaaact tcatcgcttg
     5581 ttgcagtaaa caggtttgat aaatctattt tattatcgcc cgtatcaaaa acaacggatt
     5641 tttcaaggtt tgaatagtac cattcccgtg ttgcggcata gacctcaccg ccgaataaat
     5701 taagcaaaaa agccgcaaga gctataataa tacaacacac aagttgaatc gttatcacaa
     5761 gctgtttcgg agaatttttc ttttcatcac tattttcagc cgataaagag ttcacatcct
     5821 cctcatctaa atcaagctca tccgatacct tcgggtattc cgaatatgaa acagatttcc
     5881 agtcatcgtc atatgtaatg gtatttctgc tatcattatt ttctgtcaaa aaaatcacct
     5941 caatgcaata tatgcaatga ggtgaacaaa tttaactgcg tcattcaatc ttttggtcaa
     6001 ggctcttgaa atcgcctttt gtaaggttga caaaaaatgc aattgcaagc attaacgaga
     6061 aaaattccgc aatcgggaaa gcataccata cggctgtata atcaaacacc atagaaagca
     6121 cccacgcaat cggaagaatg agtacaagct gacggcagaa tgacataata agactgcgca
     6181 gtccctttcc gaccgcctga aaaagtgttg taaagataat gccggcagca gcaggaataa
     6241 agcaaaggct tatgattctg aatgcaggga caccgatatc cataatatcc tgtgtgccac
     6301 caaacatagc aataagctgt tcggggattg tccacataag aattgtgccg accgccataa
     6361 taattacgcc aatgatgatg ccgcgcttta aagcggaata cattctcttt ttctttcttg
     6421 caccgtagtt ataaccgata atcggcatta cgccctgatt gagtccgaaa caaggcataa
     6481 atacaaagct ctgtaattta taatatatac cgagaacagt aacggcagcc tccgaaacag
     6541 caagaattgc attgagtccg ataatcataa cggaacctat tgactgcata attacagacg
     6601 gaaagcctac aacataaata gatttcaatg tggaaccatt caatttgaaa tgtttgaacg
     6661 aaactttaac ttcgttattc ttaaagaaaa gaatgcaaag gcagaaaacc ataccgcaga
     6721 attgaccgaa tacagtagca attgccgcac cgagaacgcc catttcaggg aaaattccta
     6781 taccgaaaat caggagcggg tcaaaaataa tatttacaat tgcaccgaaa agctgggata
     6841 acatcgggaa tatcatattg cctgttgcct gaagcgtttt ttcaaccata atctgtacaa
     6901 ttgaaaagaa cgagaagcaa agtacaaccg tcagatactg gattccgtaa tttacaatag
     6961 cctcattctg cgtgtacatt gccataaacg ggcgaaccgc aaaaagtccg agcaataaaa
     7021 atattacata attaaaaata caggttacaa gaccgtgcgt tgctccgtcg tnnnnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg actgccgtac cggctgcaaa cgcaatcaaa
     7141 agcatttgca gaggatacgc aagcgaagtt gcggtcagtc cgtctgcgtc atagttgccg
     7201 ataaaaatac tgtcaacaac attatacatt gcctgaattg tcattgaaaa aattgcaggc
     7261 agactcattg ttagaattag ccttgtcatt ggcatagttc ccattttgtt ttcatggggc
     7321 tttactgaat tttccatatt tcccatactg attcctttct tattcgcata catatagtta
     7381 gttgatcttt cgtatcttcc tgtaaatcca aaatgcaaca agcattacag ccgtaccaag
     7441 caacacaccg aaaaagtcaa tccacatatc gctcacctgt cccgatcttc cctcaatccc
     7501 aagctgaatt gtttcatcaa caaaacaggt aaacagtccg atagaaacgg tgtagggtac
     7561 aaattttatc ggcttgattc ggtcaaatga ataggcacat gaacagagca atgctcccag
     7621 aacggtatat tccgtaaaat gagcaagctt tctgattatg tgatccgaaa gattcggatc
     7681 gattccgaaa aatttcaaaa ttactgtgac aaaatcaagg acgctttcac tctcttgtgc
     7741 cgactcaact gacggcattg aagaatgaat aaacgcaaat gcaatcactg acgcggtcaa
     7801 aacaaacata attattctaa atcgcattga acaggaattc gttttcatca aatcactcct
     7861 aaatatattt tacagattat tcagtcaatg tcaacttttg aataattatt tatcataatg
     7921 tataaataaa tcaaatttta aggctttatt atggaagatt ttatcttgct caaatattga
     7981 aaaaaacttt taaatctgtt atacttatca ggtattggca aaatataatt tctaaggagc
     8041 aaatatgtgg tatttaatag tattttttat ttcaatggtt ccgatagtag aaatcagagg
     8101 tgctgtgcct gttgcggttg caaatgactt aaatcttttt tggacatatg taatctgcat
     8161 tttaggcaat atgctcccgg ttccgattat ctacctcttt gcaagaaagg ttcttgtttg
     8221 gggttctgac aagaagttta tcggcagatt cttttctttc tgtcttgaaa aaggcgaaaa
     8281 aggcggcaga aaacttgagg caaaagcagg caacggaatg tttgttgcac ttatgctttt
     8341 cgtaggcatt cctcttcccg gcacaggcgc atggacagga acacttgcag caagtattct
     8401 tgacctcgga tttaagaaaa gcgttatagc cgttatgtgc ggtgttgtta tcgcaggtat
     8461 tataatgggt atcggttcgg tacttgttgc aaacggaatc aactttattt ttgctccgta
     8521 aataattttg ggcagcacat cgctgccctt attttttggt gaatttataa aatggaaaag
     8581 aaaaatatat atgatgcaat tataatcggc ggcggtgcat cgggacttat gtgtgcaatc
     8641 acagcaaaga aacatgaccg ttcaaagaaa attgcaataa tcgaaaaaaa tgacagatta
     8701 ggcaaaaagc tgatgtcaac aggcaacggc agatgtaacc tgacaaatca ctgtatatct
     8761 cccgataaat atgtcggttc tttcaaaaag cagagtaaaa aaatttttga aagattcagc
     8821 ggtgatgaga tggcgaatat ttttaaaaac ttaggtcttt taagttttta cgataacgag
     8881 ggcagatatt acccgataag caaacacgcc gnnnnnnnnn nggatgttct gcgcttacag
     8941 gttgaaacac tcggaataga tgttttcaca aaacagaatg tcaattctat aaaaaaagtt
     9001 tcaaacggtt ttaaaatttc gtctgatgac agcgaaaaaa agtacgattt catatgtaac
     9061 aagcttgtga ttgcaaacgg ttcaaaagcc gcacctaagc ttggcggaaa cgcaagtgca
     9121 attgattatc ttaaaaattt cggtcataag gttgtttcgt tctcccctgc tttatgccct
     9181 gttaaggtta aaagcgatgt tctgaaaaca ttaaaaggtc tgagagttac gggcaaggca
     9241 cagttatacg gtgaacacgg tcgattaatc aaggaagaaa caggcgaaat tcaatttacc
     9301 gagaattcgc tgtcgggaat ttgcgtgttc aatctttcat tgtttgctaa acaaaacgac
     9361 aaaatagtgc ttgatttatt gcccgattat tcggaaaatg agctgattaa aataatcaga
     9421 caacatatat cattgttttc tgatttgccg tgtgaacagc ttttgacagg gatttttcag
     9481 aagagaatcg gacaggcaat tctgaaaatc agccgagttg atttgtcaaa gatttgttcc
     9541 aagctttcaa aggacgaagt atcaggaatt tgcaaaacaa taaaatcgat gtcatttaca
     9601 gtaagcggaa aagaagattt ctcacacgca caatgtgctt tgggcggtgt tttaggcaaa
     9661 gagattgacg aaaacactat gatgagtaaa attgtgaaaa atctttttat atgcggtgag
     9721 gcgattgacc tttgcggtga atgcggagga tttaatcttc attttgcatt tgccggcgga
     9781 attattgcag gagaaaattt atgatcagag tgaacaatat acatttaagc cttgactacg
     9841 atgataaatc ggttcgcaag gcagttgccc aacagctgag gattgaagaa aaatccataa
     9901 aaagctgtaa gatttttcgc agatcggttg acgcaagaaa aaaggataat atctactttc
     9961 ttacaaccgt tgacgctgaa cttaatatca acgaggaaaa ggtatgtaaa aaatgcggta
    10021 acgctcaaat tgcgagaagc ggtgcaaagc caattcttat tgaaagaggt cgtgatgttg
    10081 acagacgaac tgccgatgtg aacaggttct ggacaagcgg taagcttgac ataacttcaa
    10141 atgttcagtt tggcgaaggc ggtgcgggaa cattttctga cggcaaactg aattcaggta
    10201 caaaagatat aagacaacgc aaggtgcttg aagaatttgt aagccacggc gcacctgatg
    10261 aaattctcta taacgcaaag ccgcatatcg gaacggatat gctaaaagga acaataaaaa
    10321 atatccgcaa cgagattatt gaactcggcg gaagggttat gtttgaaacg aagcttgttt
    10381 caatggcttt tagtgataac aagctgaaat ctataactgt tgaaacggct aacggcgatg
    10441 aaattataga aacagacaat gttattcttg caatcggtca cagtgcaagg gatacttttg
    10501 agatgcttta tgatttaaag ttgccgattg aggcaaagcc tttctcggta ggagcaagaa
    10561 ttgaacatct tcgtcaaaaa gttgataagg cacaatacgg caggtttgcg ggcaataaaa
    10621 aactcggctc ggcaaattat aagctgagta cgcaccttga taacggacgg ggtgtgtata
    10681 cattttgtat gtgtccggga ggcaaggttg tcaatgcgtc aagcgaagaa aacagacttt
    10741 gcaccaatgg tatgagtgaa tttgcacgaa atgccgaaaa ttcaaattca gcgttgcttg
    10801 taggaataaa cccggatgat tatgaatccg accatccgct tgccggaatg tatttgcaga
    10861 gaaagcttga atcaaaagca ttcgttgccg gcggtgaaaa ttataatgca cccatacaaa
    10921 gagttgacga ttttcttaat aatagaaaat caactcatct tggtgatgta aagccaagta
    10981 tcggtccgaa ttatgagttt tcaaatctga acgagatttt gcctgaatat gtctgcactt
    11041 ctatggctca gggcattgtg aaaatgggca gaatgttaca cggatttgat gacggtgacg
    11101 ctgttttgac cggcgttgag agcagaagct cttcccccgt cagaatcatg agaaagacag
    11161 atacgctcga aagtgtactt attgaaggac tgtatccgtg cggtgaaggt gcaggctatg
    11221 caggcggaat tatctccgcc gcagttgacg gcattaaatg tgccgaaaaa attatagaaa
    11281 aatcgaataa ataaacctcc gaaatcaatc ggattgtgaa acgcccccta aaggtcagag
    11341 caaaaatctg acttttaggg ggcaatcatt ttttacaggc ggtttattta gtattgtgat
    11401 ttaaacaaat aacagtaccg ccccgacagt aaaaatcaaa acagcaaaat gtgcttgatt
    11461 gtgcggggcg gctcttagat aaacttatta aattttactc ggagtattta ctcgactgtc
    11521 cgtttgagta aatcatattt acatcgggga tttttaagtc acctgtctgt gctgaaccgt
    11581 tattgaatat agcatattct gcaccattcg gaagttcaaa tctgtatgtg ttattaccta
    11641 tactttccat agccttgccg ggccagctca tataagtcga atcttttgaa ctccatacat
    11701 agacatttac tgttgaccag ttttccgtat tattgaaata aacataattc ttcgtctgaa
    11761 ttgaagtcgg ttcggttgtt ggctctgtga catttgttgt aggctgggtg ggtgcaacat
    11821 tttctgtgta agtaccgcag tatgagtctt caacaataag cttaacgata taagcctgaa
    11881 tcatagttgc gtcttttaca gaaataacag aatccttgtc tacatctgcc gcaatgagag
    11941 cctcatcgct taaagtcgat atgttagcaa tgtatttttg aacctctgtt gaatcacaga
    12001 ctg
//
LOCUS       DAOMVE010000045        11977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1234_length_11977_cov_3.828804, whole genome shotgun sequence.
ACCESSION   DAOMVE010000045 DAOMVE010000000
VERSION     DAOMVE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_11977_cov_3.828804"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..2434)
                     /locus_tag="PLS64_03965"
     CDS             complement(110..2434)
                     /locus_tag="PLS64_03965"
                     /inference="COORDINATES: protein motif:HMM:NF039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRL43020.1"
                     /translation="MEAKTTLARRQDAVQGDFMRKMLTNAGCLLCGILVSRGAVLGSL
                     APFGASFAAAVPRKYLLSSLLGTAFGYVLLKPSDSFRYLAVVAAIGGLRWLLGDLDKV
                     TKSKVFAPLVAFVPIFATGVSLLFVSTSTLTTFADCVTEAVIAGAAAYFISTALHLAG
                     DNRSFEVFSQQETASVVMSGCILILAFGSIAWQNISLGRIIAMLVILLCSRYGSVTGG
                     AISGISTGAIFSIASRENGYICGGFAFGGLMAGLFSQLGKLGCAIAFVISNGVMCLAF
                     GSQFGTPSGVLVESLAASAVFMVLPKEVGNVISPVFSSDKNTSLGEALRKNIVMRLDF
                     ASKAVGNVKNDVSKVSEKMKKLYSPTFDAVCEGTRNEVCETCGLKMYCYEHKGGVTRD
                     DFARLEEYLESNGTIGERDVEKSFVKNCCKKGEIARSMNANYREYQSALEAQQRITDV
                     RSVVAGQFSGIGDILHDLADEFRNTMRCDNESAQRIISALTSLGAIVEECICLVSNGG
                     RMSVELTLSNKSEKLSKGEVMREISRCCGRRFDLPTISREGNRIRIAMCEMPVFDVEI
                     GSDQHTADNGKLCGDCINYFNDGFGKTYALVCDGMGTGGRAAVDGNMAASVMTRLLRA
                     GLSADSCLQIVNSALMVKSEDESLSTVDVTSVDLYTGKTTFKKAGAPVTFVKKNGRVT
                     VREMPSLPAGILNGIKFSTDTVNLTTGDMIVMVSDGVITGDDKWLEKLIRTWNEGSTQ
                     DLAKAVVDEAVKHRKADREDDVTAVAIRITENGH"
     gene            2671..3096
                     /gene="ruvX"
                     /locus_tag="PLS64_03970"
     CDS             2671..3096
                     /gene="ruvX"
                     /locus_tag="PLS64_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRL43021.1"
                     /translation="MIIMSVDLGKARTGIAVCDKTEFLASPYTVIFEKSPKQLLEKVS
                     NTAKETKAELIVVGLPKNMDGSEGESAQNARAFAENLSNETGIECVMQDERGTTITAH
                     NFLNTTNTRGKKRKNVVDEVAATIILQDYLDKRKSGLNK"
     gene            3319..3513
                     /locus_tag="PLS64_03975"
     CDS             3319..3513
                     /locus_tag="PLS64_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL43022.1"
                     /translation="MYFKRLRDLREDHDMKQSEVAEYLGIQQTVYSRYERGFQSIPVE
                     HLIKLADLYKVSTDYILGRK"
     gene            complement(3783..7376)
                     /gene="rpoC"
                     /locus_tag="PLS64_03980"
     CDS             complement(3783..7376)
                     /gene="rpoC"
                     /locus_tag="PLS64_03980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRL43023.1"
                     /translation="MDNNVFDSIKIGLASPEQMREWSYGEVKKPETINYRTLKPERDG
                     LFCERIFGPTKDWECHCGKYKRIRFKGKICDRCGVEVTRSKVRRERMGHIELAAPVSH
                     IWYFKGIPSRIALMLDISPKLLENVLYFSQYIVTDPGDCRDLAKYQCLTEGEYNDMRE
                     KYEDDFEAGMGAEAIKKLLAEIDLDALSASLKEELETASGQKRVRLLKRLDVVESFRM
                     SGNRPEWMILDVVPVIPPDIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLLELNA
                     PEIIIRNEKRMLQESVDALIDNGRRGRPVTGPNNRALKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKMYQCGLPKEMALELFKPFVMKRLVETKAEQNIKSARKAVER
                     AKDNVWDALEVVIKGHPVLLNRAPTLHRLGIQAFEPVLVEGRALKLHPLACTAYNADF
                     DGDQMAVHVPLSAEAQAEARFLMLAAGNLLKPSDGQPVTVPTQDMILGSYYLTLDKDG
                     ERGEGKVFRDVDEVMMAYQTGVIELHSKIKVRRELEIDGKIESGIVDTTIGKIIFNNP
                     IPQDLGFIDRSIPENRFKFEVDFLVGKSGLKKIIDKCIKIHGPVRTSVVLDHIKAQGY
                     KYSTKGAITVAVCDAVIPPQKKAIIAEAEKEVDVIRDEFMMGLRSNEERYEEVLRIWE
                     KATNDVTDALQKNLDRYNPIFMMADSGARGSMSQIRQLAGMRGLIANTSGKTIEIPIR
                     ANYREGLNILEYFISSRGARKGLADTALRTADSGYLTRRLVDVSQEVIIREEDCGTTE
                     GLEIFDIKAGESNVIEGLHERLIGRYLLDDFCDSNGNVLVSKDVMMGDKEADIIVNSG
                     VDHIKIRSVLECRAKHGACRKCYGSNLANRQPVTVGEAVGIIAAQSIGEPGTQLTMRT
                     FHTGGVASAEDITQGLPRVEELFEARKPKSLAIISEIDGDVRFEEIKNAKHAVIFNKE
                     TGEERAYLIPFGFRVKVFDGDHIKKGDKITDGAVNPHDILDILGPEAVMNYLISEVQS
                     TYRLQGVEINDKHIEVIVRQMMRKVKVEDAGDTGFMSGQTYDKNEVLYENEQIKKRIA
                     NGEEGLREATFTQLLLGITKAALATDSFLSAASFQETTRVLTDAAIKGKVDPLVGLKE
                     NVILGKIIPAGTGMQRYADVQLESNSEAEIVENDDTDDVIVALEDDEIMDF"
     gene            complement(7392..11117)
                     /gene="rpoB"
                     /locus_tag="PLS64_03985"
     CDS             complement(7392..11117)
                     /gene="rpoB"
                     /locus_tag="PLS64_03985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRL43024.1"
                     /translation="MVNVKPVKLGNTERMSFGKIDEVIDMPNLIDIQKASYKWLLDEG
                     LKEVFKDVSGITDYQDNLVLDFIDYTLDVDHPNYSVIECKVRDATYSAALRVTARLLN
                     KSTGEIKESNVFMGDFPLMTASGTFVINGAERVIVSQLVRSPGVYYKMDHDKTGKELY
                     STTVIPNRGAWLEYETDVNDVFYVRIDKNRKLPVTSFIRALGLGTDAEILDYFGDDER
                     MRATIEKDAASNVEEGLIEVYKKLRPSEPPTVDSAQTHINNLFFDPGRYDMSRVGRYK
                     YNKKLGIANRLEGQVLAEPIANPRTGEVMALRDEKITKEKAFEIENAGVKIAYVKAAD
                     ETIVKVISNGMVDIACYVDFDAEAECDIKENVRFDVLCEILDSCENEDDLKEMLRSRA
                     DELVPNHITVDDIFATINYLNCVAHGVGNTDDIDHLGNRRIRCVGELLQNQFRIGFSR
                     LERVVRERMTTQSQDMESLSPNSLINIRPVTAAIKEFFGSSPLSQFMDQGNPLAELTH
                     KRRLSALGPGGLSRDRAGFEVRDVHYTHYGRMCPIETPEGPNIGLISYLASFARINKY
                     GFIEAPYRKIDKETGVVTDEVVYMTADVEDNYAVAQANEPLDENGRFVHSRVVGRYRD
                     EFVEYSPERFDFMDVSPKMVVSVATAMIPFLENDDANRALMGANMQRQAVPLLRSEAP
                     IVGTGMEYKAGTDSGVCILAEEDGIVMSVDARNIRVQYDSGRVQDFEVIKFLRSNQGT
                     CINQRPIVSRGQRVKKGEVLADGPATENGEVALGKNALIGFMTWEGYNYEDAVLINEK
                     IVRDDVYTSIHIEEYDTEARDTKLGPEEITRDIPNVGEDVLKYLDEDGIIQIGSEVKA
                     GDILVGKVTPKGETELTAEERLLRAIFGEKAREVRDTSLRVPHGECGTVVDVKVFTRA
                     DSRNELQPGVNKVVRVYLALKRKISVGDKMAGRHGNKGVVSRILPMEDMPFLPDGTPL
                     DIVLNPLGVPSRMNIGQVLEVHLGYAAKALGWKIMTPVFDGAHEQDIFQCLKDAGYSE
                     DGKTWLVDGRTGERFDNPVTVGYMYYLKLHHLVDEKIHARSTGPYSLVTQQPLGGKAQ
                     FGGQRFGEMEVWALEAYGAAYTLQEILTVKSDDVVGRVKTYEAIVKGQNIPAPGIPES
                     FRVLIKELQSLSLDVRVLNSDGEEIDIKQKFDEDEDIVDATNPEFEEDHVMTSMDGYT
                     LDEGGEEGNMFDDSLADVEEDSYTSDDFSDDFGSDDM"
     gene            complement(11576..>11977)
                     /locus_tag="PLS64_03990"
     CDS             complement(11576..>11977)
                     /locus_tag="PLS64_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43025.1"
                     /translation="SKRNVKYMRKYVDYDLGINWVSHRKNLLCLFYNFGTKDTHGCQS
                     TVRPYFYGRVFKTKKGNRIIGIALPEITFLLLSLVIFIFCAADMVESFWLGLGGCIFA
                     LFVFVLFNLDMPSGISRIKDYLEKQFKSEYD"
BASE COUNT         3272 a         2819 c         2505 g         3381 t
ORIGIN      
        1 tttctgacat ctatataaat ttattttaca taaattctgt gcctttactt gcgtgtgtcc
       61 gattaacctc gctatatttt ctgtttttca atttttaact taatttgaat caatgtccgt
      121 tctccgtaat tctgattgca acggcggtga catcgtcctc acggtcggcc ttacggtgct
      181 ttaccgcctc gtcaacaaca gcctttgcaa gatcctgggt actgccctca ttccatgtgc
      241 gaataagctt ttcgagccat ttgtcatcgc ccgtaattac accgtctgat accataacaa
      301 tcatatcccc cgttgtcaga tttaccgtgt cggttgaaaa ttttataccg tttagaattc
      361 ctgcagggag cgacggcatt tcacgaaccg ttaccctgcc gtttttcttt acaaatgtta
      421 cgggtgcgcc tgctttttta aaagtggttt tgccggtgta gaggtcaaca cttgtgacat
      481 caaccgttga aagcgattcg tcctcacttt tcaccatgag ggcggagttc acaatctgca
      541 aacagctgtc ggctgaaagt cccgccctta gcagtcttgt cataacagac gccgccatat
      601 tgccgtcaac ggcagctctg ccgcccgtac ccataccgtc acacacgagc gcatatgttt
      661 tgccgaaacc gtcattgaaa taatttatgc agtcaccgca gagcttaccg ttgtcggcgg
      721 tgtgctggtc gcttccgatt tcaacatcaa acacgggcat ttcgcacatt gcaatacgga
      781 ttctgttacc ctcacggctg attgtcggaa ggtcaaatct tcttccgcaa cagcgtgaaa
      841 tctcacgcat aacctcgccc ttagaaagct tttcggactt attcgagagc gtaagttcca
      901 cgctcattct tccgccgttt gacacaaggc agatgcactc ctcaacaatt gcaccgaggc
      961 ttgtgagggc gctgattatc cgttgtgcag attcgttatc gcaccgcatt gtgttgcgaa
     1021 attcatcagc caaatcgtgg agaatatcgc cgattccgga aaactgccct gcaacaacgc
     1081 tccgcacatc ggtaatcctt tgttgtgcct caagcgccga ctgatattca cggtagtttg
     1141 cgttcattga acgggcaatt tcgccttttt tacagcagtt ttttacgaaa gatttttcaa
     1201 catctctttc gcctattgtg ccgttcgatt caagatactc ttcaagcctt gcaaaatcgt
     1261 cacgggtgac accgcccttg tgttcatagc agtacatttt cagtccgcag gtttcgcaga
     1321 cttcatttct tgtaccctca caaacagcgt caaatgtggg cgaatagagc tttttcatct
     1381 tttcagaaac ttttgagaca tcgtttttca cattgccgac tgcctttgag gcaaagtcga
     1441 gtcgcataac aatatttttt cgcagagcct ctccgagcga tgtgtttttg tcgcttgaaa
     1501 acacgggcga aatcacattg ccgacctctt tcggaagaac cataaacacc gcacttgcgg
     1561 caaggctttc aacaagcaca cccgacggtg ttccgaactg cgaaccgaac gcaaggcaca
     1621 tcacaccgtt tgagattaca aaggcaatcg cacagcccaa ttttccgagc tgtgaaaaaa
     1681 gccccgccat aagtcctccg aatgcaaaac cgccgcagat gtagccgttc tctcttgacg
     1741 caatgctgaa aatcgcccct gttgaaattc cgctgattgc accgcccgta accgagccgt
     1801 agcgtgaaca gagcaaaatg acgagcattg caataattct gccgagcgag atattctgcc
     1861 acgcaatact gccgaaagcc agaatcagga tacaccccga cataaccacg cttgcggttt
     1921 cctgttgcga aaaaacttca aaacttctgt tgtcgcctgc aaggtgcagt gccgtgctga
     1981 taaaatatgc cgccgcacct gcaattaccg cctcggttac gcaatcggca aaggttgtca
     2041 gcgtgcttgt gcttacgaaa agcaacgaaa cacccgttgc aaaaatcgga acaaatgcca
     2101 caagcggagc aaaaaccttg ctttttgtaa ccttgtcaag atcaccgagc agccaccgca
     2161 gaccgcctat tgccgccaca acggcaaggt aacgaaagct gtccgagggt ttgagcagga
     2221 cataaccaaa tgccgtgcct aagagtgatg acaaaagata ctttctcgga actgccgccg
     2281 caaatgacgc cccgaacggt gcaaggcttc cgagaaccgc tccccgtgac accaaaattc
     2341 cgcagagcag acaacctgcg tttgtgagca ttttccgcat aaaatcgccc tgaacagcgt
     2401 cctgtctgcg agccaaagta gtttttgctt ccataaaaat caacatccct ttaatgattt
     2461 acttcttttt gtatttttat tataggatat tatgggtaac agttttgtct ttttgtgaat
     2521 gagaattttt atattctttt gtagaaacaa agctttgaaa aaacttaata aagtaattac
     2581 gcaaaagatt gttcaacatt aaaaaaagcc gttgttaaaa ttctgatttt gcattataat
     2641 ggagtaaacc aaagtgaaag gacaaacaaa atgattataa tgtcagttga cctcggaaaa
     2701 gcacgcacag gcattgccgt atgcgacaaa accgagtttt tagcgtcgcc atatacggtt
     2761 atttttgaaa agtcaccgaa acagttgctt gaaaaggtgt caaatacagc caaagaaaca
     2821 aaagccgagc ttattgttgt gggacttccg aaaaatatgg acggcagtga gggcgaaagc
     2881 gcacagaatg ccagagcgtt tgccgaaaat ctgagcaatg aaacaggcat tgaatgcgtt
     2941 atgcaggatg agcgtggaac aacaattacg gctcacaatt ttctcaacac cacaaataca
     3001 agaggaaaaa agcgtaaaaa tgtcgttgac gaggttgccg ccacaataat tcttcaggat
     3061 tatcttgaca aaagaaaatc gggcttaaac aaataaaatc ataaaagatt tacccttccg
     3121 atgatttcgg agggttattt aatgtacatt ttgagaattt tataacctaa aattcaagca
     3181 cgccgcagtt tttctcactt gaaactgcgg cgtgctttta agttaaatgc tttagtactt
     3241 ttaagtacta taaaatcatt atatcaccca tttatgataa agtcaatagt accgatagat
     3301 actatacagg gtgataatat gtattttaaa agactgcgtg atttgcgaga agatcatgat
     3361 atgaaacaaa gtgaagttgc cgaatattta ggaattcagc agacggtcta ttcacgctac
     3421 gaaagaggat ttcagtcaat tcctgttgag catttaatta aactggctga tttgtacaaa
     3481 gtttccacag attacattct cggcagaaaa tagttacaaa ttaaaaatta aagtcgggca
     3541 gcagattttc aatgcaaaat ttctgtttcc cgactttttt atttgtgata cggcgagccg
     3601 ccgttccctg ttcgtgaaga cagtttgtaa tgtcaaaagc atattatgcc agagcgaaag
     3661 ttttttaaaa tggaaaattg aaaattgcgg tcgggcagac agtttgcaaa cgaaaaaata
     3721 cacggtatgt accatttggc acataccgtg gtttttttat acattatatg ttttcagtta
     3781 aatcagaagt ccataatttc atcgtcttca agagcaacga taacatcgtc tgtatcgtca
     3841 ttctcaacga tttctgcctc agaattactc tcaagctgaa catctgcata tctctgcata
     3901 cctgtacctg cagggataat cttaccgagg ataacattct ctttaagacc gacgagcgga
     3961 tcaaccttac ccttgatagc ggcatctgta agaactcttg ttgtttcctg gaatgatgcg
     4021 gctgaaagga agctgtctgt tgcaagagct gcctttgtga taccgaggag gagctgagtg
     4081 aatgttgcct cacgaagacc ttcttcgccg ttggcaatac gctttttaat ctgctcattc
     4141 tcgtaaagaa cttcgttctt gtcgtaggtc tgacctgaca taaagcctgt gtcgcctgcg
     4201 tcctcaacct taaccttacg catcatctga cgaacgataa cctcaatgtg cttatcgttg
     4261 atttcaacac cctgtaaacg gtaggtactc tgtacttccg aaataaggta gttcatgact
     4321 gcctcgggtc cgagaatatc gagtatatcg tgcggattaa cagcaccgtc ggtaatcttg
     4381 tcaccctttt tgatgtggtc gccgtcaaat acctttacac ggaaaccgaa aggaatgagg
     4441 taagctcttt cttcgcctgt ttccttgtta aagataacag cgtgctttgc gtttttgatt
     4501 tcttcaaaac gaacatcacc gtcgatttca ctgatgattg caaggctctt tggtttacga
     4561 gcctcgaaaa gctcttcaac tctcggaaga ccctgtgtga tatcttcggc agatgcaaca
     4621 ccgccggtat ggaatgttct cattgtaagc tgtgtacccg gctcaccgat tgactgagcg
     4681 gcaataatac cgacagcctc accgacagta acaggctgac ggttggcaag gttagaaccg
     4741 tagcacttac ggcaagcgcc gtgttttgca cgacattcaa gtaccgaacg aatcttgatg
     4801 tggtcaacac ccgaattaac gatgatgtca gcctctttgt cgcccatcat aacatccttt
     4861 gaaacaagaa cattaccgtt agaatcgcag aagtcatcaa ggagatatct tccgataaga
     4921 cgctcgtgga gtccttcgat aacatttgat tcgcctgctt tgatgtcaaa gatttcaaga
     4981 ccttctgtag taccgcagtc ctcttcacgg ataataactt cctgtgatac atcaacaaga
     5041 cggcgggtaa ggtaacctga gtcggctgta cgaagagctg tatcggcaag acctttacga
     5101 gcaccacgag atgaaataaa gtactccaag atgttaaggc cttcacggta gttagctctg
     5161 ataggaattt cgattgtttt acctgatgta ttggcgataa gaccacgcat acctgcaagc
     5221 tgtctgatct ggctcatact accacgggca cctgagtcag ccatcatgaa gattgggttg
     5281 tagcggtcaa ggtttttctg gagggcatcg gttacatcgt tagtagcctt ttcccagata
     5341 cgaagaactt cttcgtaacg ctcctcgttt gaacgaagac ccatcataaa ctcatcacga
     5401 ataacatcaa cttccttctc tgcctcggca ataatcgcct tcttctgagg aggaataact
     5461 gcgtcacaaa cggcaacggt aatagcaccc tttgtagaat acttatagcc ctgtgcctta
     5521 atgtggtcaa gaacaacgct tgttcttacc ggaccgtgaa ttttaataca tttatcaata
     5581 atcttcttaa gacccgattt accaacgagg aagtcaacct caaacttgaa tctgttttcg
     5641 gggatacttc tgtcgataaa gccgagatcc tgaggaatag gattgttaaa gataatctta
     5701 ccgattgttg tgtctacaat accggattcg atttttccgt caatttcaag ctcacggcga
     5761 accttgattt ttgagtgaag ctcgatgacg cctgtctgat aagccatcat aacttcatca
     5821 acatcacgga atacctttcc ttcgcctctc tcaccgtcct tatcaagtgt aaggtagtaa
     5881 gaaccgagga tcatatcctg tgtaggaact gtaacaggct gaccgtcaga aggcttgaga
     5941 aggttgcctg ctgcgagcat aaggaatctt gcttctgcct gtgcctctgc cgaaagaggt
     6001 acatggacag ccatctggtc gccgtcaaag tcagcgttgt atgctgtaca agcgagcgga
     6061 tgaagtttaa gagcacgacc ttcaacaaga acaggctcaa atgcctgaat accaagacgg
     6121 tgaagtgttg gagcacggtt gaggagaacg gggtgtccct tgattacaac ttcaagtgcg
     6181 tcccaaacat tgtcctttgc acgctcgaca gcttttcttg ctgatttgat attctgctct
     6241 gctttagttt caacaaggcg tttcataacg aacggcttga agagttcaag tgccatttcc
     6301 ttaggaagac cgcactgata cattttgagt tcaggtccta caacgataac cgaacggcct
     6361 gagtagtcaa cacgcttacc gagaaggttc tgacggaaac ggccctgctt acctttaagc
     6421 atatccgaaa gtgacttgag ggcacggttg ttaggtcctg taacgggtct gcctcgtctg
     6481 ccgttgtcga tgagtgcgtc aacggattcc tgaagcattc tcttctcgtt gcggatgatg
     6541 atttcaggtg cgttaagctc aaggagcttt ttaagtctgt tgttacggtt gataactcgg
     6601 cgatagaggt cgttaaggtc acttgttgca aaacgaccgc cgtcaagctg aaccatagga
     6661 cggatatccg gcggaattac cggaacaaca tcgagaatca tccactcggg acggttgccc
     6721 gacattctga agctttcaac aacatcaagt cttttaagga gtctaactct cttctgaccc
     6781 gatgcagttt cgagttcttc tttaagtgaa gcggaaagag catcaaggtc aatctcggca
     6841 aggagctttt tgattgcctc ggcacccata cctgcttcaa agtcgtcttc atacttctca
     6901 cgcatatcat tgtactcacc ctcggtaagg cactgatact ttgcaaggtc acggcagtcg
     6961 ccgggatctg ttacgatgta ctgtgagaaa tagagtacat tctccaaaag cttgggagaa
     7021 atatcaagca taagggcaat tcttgaagga attcccttga aataccaaat gtgcgacacg
     7081 ggagcggcaa gctcaatgtg tcccatacgc tctcttctga cctttgaacg ggttacttca
     7141 acaccgcaac ggtcacagat tttaccctta aaacggattc tcttgtattt accgcagtga
     7201 cattcccagt cctttgtcgg tccgaaaatg cgttcgcaga acaagccgtc tctttcaggc
     7261 ttgagagtac gatagttaat tgtctcgggc tttttaactt cgccgtaaga ccattctctc
     7321 atctgctcag gagaagcaag tccgatttta attgagtcaa aaacattatt atctatcatt
     7381 gcatttcctc cttacatatc gtcactgcca aagtcatcgc tgaaatcatc cgatgtgtag
     7441 ctgtcctctt caacatcggc aagtgaatcg tcaaacatat tgccttcttc gccgccctcg
     7501 tcaagagtgt agccgtccat tgatgtcatt acatggtctt cttcaaattc cggatttgtt
     7561 gcatcaacaa tatcctcgtc ctcatcaaac ttctgcttaa tgtcaatttc ttcaccgtca
     7621 gagttaagaa cacgaacatc aagtgaaagt gactggagtt ccttaataag aactctgaat
     7681 gactcaggaa tacccggagc aggaatattc tgacctttaa cgatagcctc gtaggtctta
     7741 acacggccga caacatcgtc cgacttaact gtaagaattt cctgaagtgt ataagcagca
     7801 ccgtaagcct caagtgccca aacttccatc tcaccgaaac gctgaccgcc gaactgggct
     7861 ttaccgccga gtggctgctg tgtaacgaga gagtaaggac ctgtgcttct tgcgtggatt
     7921 ttttcatcaa cgaggtgatg gagcttgagg tagtacatat aacctactgt tacagggtta
     7981 tcgaaacgct cgcctgtacg accgtcaaca agccatgtct taccgtcctc gctgtaacct
     8041 gcgtctttaa gacactggaa aatgtcctgc tcatgagcac cgtcaaatac aggagtcata
     8101 atcttccagc caagcgcctt tgcggcataa ccgaggtgaa cctcaagaac ctgaccgatg
     8161 ttcatacgag aaggtacgcc caacgggtta agtacgatgt caagcggagt accgtcagga
     8221 aggaacggca tatcttccat tgggagaatt cttgaaacaa cacccttgtt accgtgacga
     8281 cctgccatct tatcgcctac gctgattttt ctcttgagtg caaggtaaac tcttacaacc
     8341 ttgtttacac cgggctggag ttcgtttctg ctgtctgcac gggtaaacac cttaacatca
     8401 acaacagtac cgcattcacc gtgaggcaca cggagagatg tatctctgac ttctcttgcc
     8461 ttttcaccga agattgcacg aaggagtctt tcctcggctg taagctctgt ttcacccttt
     8521 ggagttactt taccgacaag gatatcgcct gctttaacct cagaaccgat ctgaatgata
     8581 ccgtcctcgt caagatactt gagaacatct tcacctacat tcgggatatc tcttgtaatc
     8641 tcttcgggtc cgagctttgt atctcttgcc tctgtgtcat attcttcaat gtgaattgat
     8701 gtataaacat catcacgaac gattttttcg ttgataagaa cagcatcctc gtagttgtaa
     8761 ccttcccatg tcataaagcc gatgagagcg ttcttaccga gagcaacttc accgttttcg
     8821 gtagcgggac cgtctgcgag aacttcgccc ttcttaactc tctgtcctct tgacacgata
     8881 ggacgctggt tgatacatgt accctggtta gagcggagga acttgataac ctcaaagtcc
     8941 tgaactctgc ccgagtcata ctgtacacga atgttacggg cgtcaacgct cataacgata
     9001 ccgtcttcct cagcgaggat acaaacgcct gagtctgtac ctgccttgta ctccatacct
     9061 gtaccgacga taggagcttc actgcggaga agcggaactg cctgacgctg catgttcgca
     9121 cccatgagag cacggtttgc gtcatcgttt tcaaggaacg gaatcatagc cgtagcaaca
     9181 gatacaacca tcttaggaga tacatccatg aagtcgaatc tttcgggaga atactctacg
     9241 aactcgtcac ggtaacgacc tacgactctt gaatgaacaa atctgccgtt ttcatcaagc
     9301 ggttcgttag cctgtgcaac agcgtagtta tcctcaacat cggctgtcat ataaacaacc
     9361 tcgtcggtta caacgcctgt ttccttgtca attttacgat aaggagcttc gataaagccg
     9421 tacttgttga tacgggcaaa tgaagcaaga tatgagataa gaccgatgtt aggaccttca
     9481 ggagtttcga taggacacat tcggccatag tgtgtgtagt gtacgtcacg aacctcaaat
     9541 cctgcacgat ctcttgaaag accgcccgga ccgagggctg aaagacgacg cttgtgggta
     9601 agctctgcaa gtgggttacc ctgatccatg aactgtgaaa gcggtgaaga accgaagaat
     9661 tctttaattg ccgctgttac gggacggata ttgatgagtg agttaggcga aagcgactcc
     9721 atatcctgtg actgggttgt cattctctct cttactactc tttcaagacg ggagaaaccg
     9781 attctgaact ggttctgaag aagttcgcct acgcagcgga ttcttctgtt gccgaggtgg
     9841 tcgatatcat ctgtgttacc gacaccgtga gcaacacagt tgaggtagtt gattgttgca
     9901 aagatatcgt caaccgtgat gtggttagga acaagctcat ctgcacggct tctgagcatt
     9961 tccttgaggt catcctcatt ctcacaggaa tcaagaattt cgcaaagaac atcaaaacga
    10021 acattttcct tgatatcaca ctctgcttct gcgtcaaaat caacatagca agcgatgtca
    10081 accataccgt ttgaaataac cttaacgatt gtttcatcgg cagcctttac ataggcaatc
    10141 ttaacacctg cgttttcgat ttcaaaagcc ttttcctttg tgattttttc atcacgaaga
    10201 gccataacct cgcctgtgcg tgggttggca atcggctctg caagaacctg accttcaaga
    10261 cggtttgcaa taccgagctt tttgttgtat ttatatctgc ctactcttga catatcataa
    10321 cgaccggggt caaagaacag gttgtttatg tgtgtctgcg cactgtcaac tgtcggaggc
    10381 tcagacggac ggagcttttt gtaaacctcg ataagacctt cttcaacatt tgaagcggca
    10441 tccttttcga tagtagctct cattctttca tcgtcaccga aatagtcgag aatttcggca
    10501 tccgtgccaa gaccgagtgc tctgataaat gatgtaacgg gaagcttacg gttcttatca
    10561 atacgaacat aaaaaacatc gtttacatcg gtttcatact caagccatgc accacgattc
    10621 gggataactg ttgttgaata cagttcctta ccggttttat cgtgatccat tttatagtaa
    10681 acgcccgggc ttcttacaag ctgtgaaacg ataacacgct cagcaccgtt gataacgaat
    10741 gtgccgcttg cggtcatcag cgggaaatcg cccataaata cattgctctc tttgatttca
    10801 cccgttgact tgttcaaaag tcttgcggtt actctgagag cagctgaata tgttgcatca
    10861 cgaaccttac actcaattac gctgtagttc gggtgatcaa catcaagtgt gtagtcgata
    10921 aaatcgagaa caaggttgtc ctgataatcg gtaatgcccg aaacatcttt aaatacttct
    10981 ttaagacctt cgtcaagcaa ccatttgtat gacgctttct gaatgtcgat aagattaggc
    11041 atatcaatta cttcatcgat tttaccaaaa ctcattcttt cggtgttgcc aagctttacg
    11101 ggtttgacat taaccatagg cgtatcactc cttaaaaaat ctatgaaacg gggcaagcgg
    11161 tactgaaaat ctgctcataa aaagctcttg acacaaaagg atttttgtga taatatcccc
    11221 ttgtcggata aagttttttg cggattttcc cgacctcttc ggtacatatc cccattatgg
    11281 tacattttat tattctatat tgaaataatc aataagtcaa gagaattttc ggattttttt
    11341 cggattttac tgactttttt atatttttac aggttttatt tcccgaaaag aattattttt
    11401 cttttcgggc tattttttac atcccaatgc aacacccgac acggtgtatg gttttaggtc
    11461 atgtaatgat aagacacaaa gagcgatata tgcactgtaa catttgcttc catatataaa
    11521 aatcgggtga caaggacttg caattttgca aatctgtcac ccgattttta ttaaatcagt
    11581 cgtattctga tttgaattgt ttttccagat aatcttttat tcggcttatg cctgatggca
    11641 tatcaaggtt gaatagtaca aatacaaaca acgcaaatat acaaccgcct aaaccaagcc
    11701 aaaatgattc aaccatatct gcggcacaaa aaataaagat tacaagcgat aaaagtaaaa
    11761 aggtaatttc cggtagggcg atacctataa ttctatttcc ttttttcgtt ttgaatactc
    11821 ttccgtaaaa atatggacgc accgttgatt gacaaccgtg tgtgtccttt gtaccgaaat
    11881 tgtaaaacag acaaagcaaa ttttttcgat gcgacaccca gtttatgcca aggtcatagt
    11941 ccacatactt tcgcatatat tttacatttc tttttga
//
LOCUS       DAOMVE010000046        11801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1278_length_11801_cov_3.147625, whole genome shotgun sequence.
ACCESSION   DAOMVE010000046 DAOMVE010000000
VERSION     DAOMVE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1278_length_11801_cov_3.147625"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..1495)
                     /locus_tag="PLS64_03995"
     CDS             complement(92..1495)
                     /locus_tag="PLS64_03995"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HRL43026.1"
                     /translation="MAKKIRVTETALRDAHQSLIATRMTTEEMLPVLEKLDKIGYYSL
                     ECWGGATYDSCLRFLNEDPWDRLRTIREHCPNTKLQMLFRGQNMLGYRHYADDCVEYL
                     VQRSIANGIDIIRIFDALNDIKNLKTAIKAANKEGGHAQVAISYTTGPVFTDEYYVEY
                     AKRIADAGADSICIKDMAALLTPTRTESLVKAIKAAVPELPLQLHTHYTSGLASMCLL
                     KGVEAGADGIDTAISPLALGTSHAPTESMVAALSGTEFDTGLDLKQFSDIRAYFMTLR
                     EKYIKSGLLDPKMLATDANALIYQVPGGMLSNLLSQLKQAGKEDKLDEVLAEVPRVRK
                     DSGYPPLVTPTSQIVGTQAVFNVITGKRYSMCTNEFKGLVAGEYGTTPMPIDPAFQKK
                     IIGDKKIIKGRPADQLKPELDKLRGEIEQWIEQPEDVLSYAQFPKVALDFFEKRRNAK
                     VGINGDKLDKKNMVHPV"
     gene            complement(1526..1891)
                     /locus_tag="PLS64_04000"
     CDS             complement(1526..1891)
                     /locus_tag="PLS64_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2118 domain-containing protein"
                     /protein_id="HRL43027.1"
                     /translation="MKNLRITVNGTAYDVQVEELGGSSAPAAAAPAPAAAPAPAAKPA
                     APAGAQGSVVVKAPMPGTVVNVVVSAGQAVKAGDDLVFIEAMKMETPVKAPQDGTVAT
                     VDVAKGESVDSGKVLVTLN"
     gene            complement(1930..3243)
                     /locus_tag="PLS64_04005"
     CDS             complement(1930..3243)
                     /locus_tag="PLS64_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HRL43028.1"
                     /translation="MSIEKINQAKAEIAATSAYKTITAFFDADSFCEIDAFAKSGEGF
                     AEVVAGFGTVEGMPVYAFAQNSDICGGAMSKAQAAKLKKLYGLALKTGAPVVGFYDSV
                     GGRLNQGTELLAGCGDVLNAAASLSGVVPQISVVLGTCLGTNALNAASADIVIMSKDA
                     QLSLSVTGKHSDAAYNAENGIASIVCDDADKAIKAAKELILYLPSNNLTMAPQSFEEA
                     PAEDGCGCVVSRTVDGNSIVKLFNDYGKEANVRFARLGGQVVGIVVTKGKELGCKSAN
                     KVAKFVRFCDAFSLPVVTFVNCPGFESIKSATKASAAYAEATTVKISVVTGKAIGSGY
                     VALAGTGANADVVYALPDAVISPVNVEAAAFIMAPEVMNVATDKQAEVAQKFADENLS
                     AYNAAQNGYVDGIVEEAQLRQTLISALDMLSGKRVSTLSKKHSTI"
     gene            complement(3474..4223)
                     /locus_tag="PLS64_04010"
     CDS             complement(3474..4223)
                     /locus_tag="PLS64_04010"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRL43029.1"
                     /translation="MAKIEPIRLQKFIAQCGIASRRKAEELILGGHVKVNGKTAILGD
                     KVLPTDKVFVRGKRIVVPKGGNYRYIMLNKPRGFITTMNDERGRKCVAQLVENVGERV
                     YPIGRLDKDSEGMLVFTNDGDFANKVMHPKNNVYKIYRVTVRPSIDEDQLVKLETGVE
                     LDGRKTAPAYVHVIHKEQGRVVLEMILHEGKNREIRRMCEAVGLEVARLKRTQIGGVK
                     MGMLKQGDWRDLTEQEVKKLLANPFLNGKQL"
     gene            complement(4267..5391)
                     /locus_tag="PLS64_04015"
     CDS             complement(4267..5391)
                     /locus_tag="PLS64_04015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRL43030.1"
                     /translation="MKKIISLAVCFVMPFVLMGAKTAEDTPPTTSAQAFVLYCPDNNT
                     VICSKNADERMKPASTTKIMTSLITLEEAASCNSEVTFKQEMVAEGSSMYLKVGEKVR
                     LSDLASGMMMASGNDAANAAAYTISGSPEKFSQRMNEKAKQIGMTNTNFVTPSGLDDD
                     NHYSSAKDMALLMSYALENDDFANLTAKKSVTVEFLEPKSKKTAYANHNRLLSLYPYC
                     TGGKTGYTTVAGRCLVSSAKKDGVTLVCVTLNDRNDWNDHISLYDYAFEKYRGVDCKD
                     ADFCADIPCVGGDKDSVTVTGEKNNTIVLKREDCDKIKKKIFIDSFLYAPIEKNESVG
                     RIEYTLGGKLLYKCNIVAGEKIKSDKKSVSIFSAIGNLFS"
     gene            complement(5443..5868)
                     /gene="ytfJ"
                     /locus_tag="PLS64_04020"
     CDS             complement(5443..5868)
                     /gene="ytfJ"
                     /locus_tag="PLS64_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HRL43031.1"
                     /translation="MAMEHPIGSLMDTTMEKIKEMIDVNTIIGEPITSPDGTLIIPVS
                     KVSYGFAAGGSDLPTKKENKDCFGGGSGAGVTIQPVAFLTVYQGDVRLVSVDREEGTA
                     DKLVNMIPDVLKKVKGVFKKDKSESADDFSEITQDDIDG"
     gene            complement(5893..6513)
                     /locus_tag="PLS64_04025"
     CDS             complement(5893..6513)
                     /locus_tag="PLS64_04025"
                     /inference="COORDINATES: protein motif:HMM:NF022615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HRL43032.1"
                     /translation="MLWLYILLGIVLFIALVMFIPITLRASYKEEFWCAVYIGFVKLQ
                     LVPAKPKKEKKKKKAKKQTQKTEQPKKATEKKPSLIKKYGIEWLLNLIKKVAELAVSA
                     LQDFFSHILVKKLSLSISVAGDDAADTAIKYGKYCAVVYPAVGTIVRVVKCKGYGVDI
                     NPNFSEKAETEINFDFAARIFAFRLIGLAVKHGIKGIKLLAEVKND"
     gene            complement(6533..7147)
                     /gene="scpB"
                     /locus_tag="PLS64_04030"
     CDS             complement(6533..7147)
                     /gene="scpB"
                     /locus_tag="PLS64_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRL43033.1"
                     /translation="MAVENIRGAIEAILFANGASVEFSRIAQALEISEKKVKEHISAL
                     IDDYEASGRGITIILLDNSCQMVSKKEFAPQIRTVMDLRRNTPLSQAALEVLAVVAYN
                     QPVTKAFVEQVRGVDCSGVIGSLTTKGLVEEKGRLELPGRPLLYGTTENFLRCFNISS
                     LDELPPLPESDEDKESEENGEEQEKTENGNEQIDLLADQPVDRQ"
     gene            complement(7147..7920)
                     /locus_tag="PLS64_04035"
     CDS             complement(7147..7920)
                     /locus_tag="PLS64_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRL43034.1"
                     /translation="METQQLQYKLSAFEGPLDLLLTLISKNKIDIYDISISELLDQYM
                     EQINMMQENQMDIASEFLTMASRLVYIKSVMLLPKYEEEAEELKKELSGQLLEYAVCR
                     EMAAKLGDIYDFDSFYRDASPVEFDLTYNRIHPSEDIAKAYVSAVGRGKRKLPPSKDA
                     FSGIVSHKIVSVNSKIIHLMRRLWHGKRLEYHEIFEVCEGKSDLVATFLATLELVKGK
                     RIRIEGSGENAVVHMIEDKNAMPLPQVDEDENNENEEVQ"
     gene            complement(7965..8663)
                     /locus_tag="PLS64_04040"
     CDS             complement(7965..8663)
                     /locus_tag="PLS64_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRL43035.1"
                     /translation="MLSLLLNGIRNGNLDFGSVIAEILAVLVIIFLILPFHEWAHAFT
                     ASLLGDKAVKGRGRLSINPLSHIDPMGALCMLLFGFGWAKPVPIDPRNFKNPKVGMAI
                     TAIMGPIANIVAAFVGLLIYYPIILFTGAFPLSTIGGYVVQFLSFYISCNVGLAVFNL
                     IPIPPLDGSKVLFVFLPDKAVEFFYRYQMYFFIGLFVLLYVGILNTPINWLSNQILNF
                     ISWLASLPYMPFVG"
     gene            8863..9051
                     /gene="rpmB"
                     /locus_tag="PLS64_04045"
     CDS             8863..9051
                     /gene="rpmB"
                     /locus_tag="PLS64_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRL43036.1"
                     /translation="MAKCEFCGKDVKFGIKVSHSHRRSNRTWKPNVQRVKAIVDGSPK
                     RVYACTRCLRSGKVTRAN"
     gene            complement(9197..10801)
                     /locus_tag="PLS64_04050"
     CDS             complement(9197..10801)
                     /locus_tag="PLS64_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43037.1"
                     /translation="MSKKVISLLLAVMLTAAMIVVAAISVSADTDDEGSYVPSEGTET
                     YHIYFCKPDDWENKYTSDAGVYWENGSDTPSAWPGYSARNADIKNVYCSDVPTDVDKV
                     IWNNALKGTDDPTNPEYKLAYRTTDINFKGYDKGESDYYPDGVESFDNMIYIINPEFT
                     SENDKDELIFTGEWFYYYGNGEYGNAPKKADLKAGDYYGKDGVFPTEYNPSGEETTAE
                     STTEVVTESETTESATDGAAATEGTTSSAESSLALTVNATSNYFPMASAKYNAVTNQV
                     TVTYFMQSQKNVLDTQWHISYDPSILSVADVNTAKSVCPTMDGRGVYVNFKNKSEGVG
                     YVRFGASNLGLYDFSEKTLFATVVFDVKDISKVAPVSTTVDLVVEVLRVSEVNPDTEM
                     TDGSKEVILVDKEVVKKAPVAAAVKNDLSTELTPSTYAEPTTAEPSTAESSTEATKAT
                     EKTQESTKDTIAPVTSQDATSVTQPVTKKISSTSDTPDTSDNGNNNNANAAVQTGEAP
                     LAVVILTLLIGSTCVMFVLRKKEEIL"
     assembly_gap    11042..11051
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11160..>11801)
                     /locus_tag="PLS64_04055"
     CDS             complement(11160..>11801)
                     /locus_tag="PLS64_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43038.1"
                     /translation="QFKAAKQTADVSCQYYSEGDNEYYDSLLDGKFWAKAQEAFDGDD
                     KTFLGKFADNFFEDPDWGISMYLTNMIYVIDPTNTSVSVTGKETYAGEFYFYYGLNEE
                     TGKYEYGSLPNYDMAKEAGLVQTVDKNAKPATPDQPTTTTPSNNNNTDPTKNTVPTAG
                     QNPTSSANAVSTADTAKSTDNSAVQTGATTAAAVLAVLLVAGGAVLYTRKRFE"
BASE COUNT         3225 a         2715 c         2408 g         3443 t
ORIGIN      
        1 aaaaaagagg tgattttcaa gcaactttag tttacaaaaa atgccaccct cgttaatgaa
       61 ggtggcataa atgttaattt tagttgtcaa atcaaacagg gtgaaccata tttttcttgt
      121 caagcttgtc gccgttgatg ccaaccttgg catttcttct cttttcaaag aagtcgagag
      181 caaccttcgg gaactgtgca taagaaagaa catcctcagg ctgttcaatc cactgctcaa
      241 tctcgccacg gagcttatca agctcaggct taagctgatc tgcaggacga cctttgataa
      301 tcttcttgtc accgataatc ttcttctgaa acgcaggatc aatcggcatt ggagttgtac
      361 catactcacc ggcaacaagt cccttgaact cgtttgtgca cattgagtag cgcttgcctg
      421 tgattacatt gaatacagcc tgtgtgccga caatctgtga tgtcggagta acaagcggag
      481 gataacctga atccttacga acacggggta cttctgcaag aacttcgtca agcttgtctt
      541 ctttaccggc ctgcttgagc tgtgaaagaa ggttggaaag cataccgccg ggaacctgat
      601 agataagagc gttagcgtct gttgcgagca tcttaggatc aagaagtcct gacttgatgt
      661 acttctcacg gagagtcata aagtaagcac ggatgtcaga gaactgttta aggtcaagac
      721 ctgtgtcgaa ctctgtgcct gaaagagcgg caaccattga ctctgtaggt gcgtgtgatg
      781 taccgagagc gagtgggctg attgctgtgt caatgccgtc ggcacctgcc tcaacaccct
      841 ttaaaagaca cattgatgca agacctgatg tgtagtgggt gtggagctga agcggaagct
      901 ccggaacagc ggctttgata gcctttacaa ggctttcggt tcttgtcgga gtaagaagag
      961 cagccatatc cttgatacag attgaatctg cacctgcgtc tgcaattctc ttagcatact
     1021 caacatagta ttcatctgtg aatacaggac ctgttgtgta gctgatagca acctgtgcat
     1081 gaccgccttc tttgttagcg gccttgatag ctgttttaag attcttgatg tcgttaagag
     1141 catcaaaaat acggataatg tcaataccgt ttgcgattga acgctgaaca aggtactcaa
     1201 cgcagtcatc ggcatagtga cgataaccga gcatattctg tcctctgaaa agcatctgga
     1261 gctttgtgtt agggcaatgc tctctgattg tgcggagtct gtcccacgga tcttcgttta
     1321 agaatctgag gcatgagtca tatgttgcac cgccccagca ttcgagtgag taataaccga
     1381 ttttatcgag tttttcaaga acagggagca tttcctctgt tgtcattctt gttgcaatga
     1441 gtgactgatg agcgtcacgc agagcagttt cggtaactct gatttttttt gccattttac
     1501 agcatccctt tctccgaatt attgattagt taagtgttac gagaacctta cctgagtcaa
     1561 cagactcacc ctttgcaaca tcaactgttg caactgtgcc atcctgcgga gccttaacgg
     1621 gagtttccat cttcatagcc tcgatgaata cgaggtcatc tccggcctta acagcctgtc
     1681 ctgccgaaac aacaacatta acaactgtac cgggcatagg agcctttaca acaacagaac
     1741 cctgagcgcc tgcgggagct gccggctttg ctgcaggagc gggagcggct gccggagcag
     1801 gagcggctgc tgcgggagct gaagagccac cgagttcttc aacctgaaca tcatatgctg
     1861 tgccgttaac tgtaattctt aaattcttca tttttagtat ccccttttat tttaacaagt
     1921 aatcatttat cagatagttg aatgcttctt tgaaagtgtt gaaacacgct tgcccgagag
     1981 catatcaaga gcagaaataa gagtctgacg gagctgagct tcttcaacaa taccgtcaac
     2041 atagccgttc tgagcggcat tgtaagcaga aagattctcg tcagcaaact tctgagcaac
     2101 ctcagcctgc ttatctgtgg caacattcat aacttcggga gccataatga atgcggcagc
     2161 ctcaacatta acaggtgaaa taaccgcatc gggaagtgcg taaacaacat ctgcattagc
     2221 gcctgtgcct gcaagagcaa catagcctga accgattgcc ttgcctgtaa caacagaaat
     2281 cttgactgtt gttgcctcag cataagcggc tgacgccttt gttgccgact tgattgactc
     2341 aaagcccggg cagtttacga atgttacaac agggagtgag aaagcgtcgc agaaacgaac
     2401 aaactttgct accttgtttg cagacttgca accaagctcc ttgccctttg taacaacaat
     2461 acctacaacc tgaccgccga gtcttgcaaa acgaacattt gcttccttgc cgtaatcatt
     2521 gaagagcttt acaatgctgt tgccgtcaac tgttctgctg actacacaac cgcaaccgtc
     2581 ctctgccgga gcttcttcaa atgactgagg agccattgtg aggttatttg acggaagata
     2641 aaggataagt tcctttgcag ccttgattgc cttgtctgcg tcgtcacaaa cgattgaggc
     2701 aataccgttt tcagcattgt aagcggcatc ggaatgcttg cctgttaccg aaagtgaaag
     2761 ctgtgcgtct ttgctcatga taacgatgtc tgcgcttgcg gcgttaagag cgtttgttcc
     2821 gaggcaagtg ccgagaacaa ccgagatctg cggtacaaca ccggaaagtg acgcagccgc
     2881 attgagaaca tcaccgcaac ctgcgagaag ctctgtaccc tgattaagtc tgccgccaac
     2941 gctgtcatag aagcctacaa caggagcgcc tgtcttgagt gcaagaccat acagcttttt
     3001 aagcttggca gcctgtgcct tactcatggc accgccgcaa atgtcgctgt tctgtgcaaa
     3061 cgcataaaca ggcatacctt ctactgttcc aaagcctgca acaacctcgg caaaaccttc
     3121 accggatttt gcaaaggcat caatttcaca aaagctgtct gcatcaaaaa acgccgtaat
     3181 tgtcttataa gcggaagttg cggcaatttc agcctttgcc tgattgatct tttcaatact
     3241 catcttaaaa tcctccaata ccatgtattg aatatgcacg aaaactgagc gtgcatacta
     3301 attttctaca caatacatta tactctataa tcttcaaaag tacaagaaaa acacgctgta
     3361 ctatttcaat tttgaacaca tttaatagtt attacaccaa aaccgtaaat ttattgtcga
     3421 attaacgaat agttttattg accgtttcgg aagtattttt caaaaacgga aaattaaagc
     3481 tgtttgccgt ttaaaaacgg gtttgcaagc agttttttaa cctcttgttc ggtcaaatct
     3541 ctccagtcgc cctgtttgag catacccatt tttactccgc cgatctgggt gcgtttgagt
     3601 cttgcaacct caagcccgac agcctcgcac attcttctga tttcacggtt cttaccctcg
     3661 tgaagaatca tttcaagaac tactctgccc tgctccttgt gaattacatg gacatatgca
     3721 ggagcggttt tcctgccgtc aagctcaacg cctgtttcaa gctttacaag ctgatcctcg
     3781 tcaattgacg gtcgaacggt tactctgtaa atcttgtaaa cattgttttt cgggtgcata
     3841 accttgtttg caaagtcacc gtcatttgta aaaacaagca taccctcgct gtctttgtca
     3901 agtctgccta tcggataaac tcgctcgccg acattctcaa caagctgtgc aacacacttt
     3961 ctgccacgct catcattcat tgtggtaatg aatccacggg gcttgttgag cataatatat
     4021 cggtaattac cgcccttcgg cacaacaatc ctcttgcctc ttacaaacac cttgtctgta
     4081 ggaagaacct tatcgccgag aattgcggtt ttgccgttca ccttgacatg accgccgaga
     4141 ataagctcct cggcctttcg ccttgacgca ataccgcatt gcgctatgaa tttctgaagt
     4201 cttataggtt caattttagc catatgtaaa ttccttttta ttaaaatttt ctgtatattt
     4261 aagctgtcac gaaaacagat ttcctattgc cgaaaatatt gacacactct ttttatccga
     4321 tttaatcttt tcgcctgcaa caatgttgca tttataaagc agctttccgc cgagtgtgta
     4381 ttcaattctg ccgaccgatt cgttcttttc aatcggagca tacaaaaagc tgtctatgaa
     4441 gatttttttc ttgattttat cgcagtcctc acgctttaaa acgattgtgt tgttcttctc
     4501 acctgtaacc gttacgctgt ccttatcacc gcccacgcac ggaatatcgg cacaaaagtc
     4561 agcgtccttg cagtcaactc ccctgtactt ttcaaaagcg taatcataaa gagaaatatg
     4621 gtcattccaa tcgttgcggt cattcagagt gacgcacaca agcgttacac cgtctttttt
     4681 tgcagaggaa acaaggcacc gccctgccac cgttgtatag cctgtttttc cgcctgtaca
     4741 gtagggataa agcgacaaaa gcctgttatg atttgcatat gcggttttct ttgatttcgg
     4801 ttcaagaaat tcaacggtca cgctcttctt agccgtaaga tttgcaaaat cgtcattttc
     4861 aagtgcatat gacataagca gagccatatc ctttgcactt gagtaatggt tgtcatcgtc
     4921 aagtccgctc ggcgttacaa aattggtatt cgtcataccg atttgctttg ccttttcatt
     4981 cattcgttgt gaaaactttt cgggactgcc cgaaatcgta tatgccgccg cattcgccgc
     5041 atcattaccc gacgccatca tcattcctga cgcaaggtcc gaaagtcgga ctttttcacc
     5101 gactttaaga tacatcgaac tgccctcggc aaccatttcc tgtttgaagg ttacttcgct
     5161 gttacaggat gccgcctcct caagcgttat gagcgaggtc ataatctttg tggtgctcgc
     5221 aggcttcatt ctttcatctg cattttttga acagataacc gtgttgttat cgggacaata
     5281 aaggacaaat gcctgtgcag aagttgtggg aggagtatcc tccgcagttt ttgcgcccat
     5341 aagcacaaac ggcataacaa agcacaccgc aagcgagatt atttttttca taaggacacc
     5401 ccctgcataa atctatgccg aggggtgttg caatattacg aatcagccgt caatatcgtc
     5461 ctgtgtgatt tcggaaaaat cgtctgcgga ttcgctctta tcctttttga aaactccctt
     5521 tactttcttg agaacatcgg gaatcatatt aacgagcttg tcggctgtac cttcttccct
     5581 gtcaacggaa acaagcctta catcgccctg ataaacggta agaaatgcaa caggctgaat
     5641 tgtcacacct gcaccgctgc cgccgccaaa gcagtctttg ttttcctttt tggtaggcag
     5701 atccgaaccg cctgccgcaa agccgtagct tacctttgaa acaggaatga tgagtgtgcc
     5761 gtcaggcgaa gttataggct cgccgataat agtattgaca tcaatcattt ccttgatttt
     5821 ttccattgtg gtatccatta aactgccgat aggatgttcc atagccattc tcctttttca
     5881 acaatattta tatcaatcat ttttaacttc tgcaagaagt ttaatcccct tgattccgtg
     5941 cttaacggca agaccgataa gtcggaatgc gaaaattctt gcggcaaagt caaagttaat
     6001 ttctgtttct gctttttccg aaaaattcgg atttatgtca actccgtagc ctttgcattt
     6061 tacaacacgg acaattgtgc ctactgccgg ataaacaacg gcacagtatt tgccgtattt
     6121 tatagccgtg tcagccgcat cgtcacctgc aacgctgata ctcagcgaca attttttaac
     6181 cagtatgtgg ctgaaaaagt cctgcaatgc agatactgca agctctgcaa ctttttttat
     6241 caggttcaaa agccactcaa taccgtattt ttttataagg ctcggctttt tttctgtcgc
     6301 ttttttaggc tgttcggttt tctgagtttg tttttttgct tttttcttct tcttttcctt
     6361 tttgggtttt gccggcacaa gctgaagctt tacaaatccg atatacacgg cacaccaaaa
     6421 ctcctcttta tatgatgcac gcaaagtaat cggaataaac atcacaagcg caataaacag
     6481 cacaattccg agtaggatat atagccaaag caaagggaat tccccctaaa ttttactgcc
     6541 tgtcaacagg ctgatcggca agcaaatcaa tctgctcatt gccgttttca gtcttttcct
     6601 gttcttcacc gttttcttcg gactccttgt cctcatcact ctcggggagc ggaggaagtt
     6661 cgtcaagact tgaaatgtta aagcaacgca aaaagttttc ggttgtgccg taaagcaacg
     6721 gtctgccggg tagctcaagt ctgccctttt cttcaacaag tccctttgtt gtcaaactgc
     6781 cgattacacc actgcagtca acaccacgca cctgctcgac aaaagccttt gtaacaggct
     6841 gattgtacgc aacaactgca agaacctcaa gagccgcctg tgaaagcggg gtgtttcttc
     6901 tcaaatccat aacggtacga atctgcggag caaattcctt ttttgaaacc atctgacagg
     6961 aattgtcaag caatattata gttatgcccc gtcctgacgc ctcataatca tcaatcagtg
     7021 cagaaatatg ctctttaacc tttttttcgg aaatttcaag tgcctgcgct attcgggaaa
     7081 attcaaccga cgcaccgttt gcgaacaaaa tcgcctcaat cgcaccacga atattttcaa
     7141 cagccattac tgcacctcct cattttcatt gttttcgtcc tcgtcaacct gtggcaaagg
     7201 cattgcgttt ttatcctcaa tcatatgaac aacggcattt tcaccgctgc cctcaattct
     7261 gatacgcttg ccctttacca gctccagcgt tgcaagaaat gttgccacaa ggtcgctttt
     7321 gccctcgcac acctcaaaaa tttcgtgata ttcaagcctt ttgccgtgcc agagccttct
     7381 cataagatgg ataatcttac tgtttaccga cacgatttta tgcgacacaa tacccgaaaa
     7441 tgcgtctttt gacggcggaa gcttacgctt gccccgtccg actgcgctga catatgcctt
     7501 tgcgatatcc tcactcggat gaatacggtt gtaagtcaag tcaaattcaa caggagatgc
     7561 atctcggtaa aagctgtcaa agtcataaat atcgccgagt tttgccgcca tttcacggca
     7621 aacggcatat tcaagaagct gtcccgaaag ttctttcttg agttcttcag cctcttcctc
     7681 atattttggc aggagcatta cagacttgat ataaacaagc cttgacgcca ttgtcagaaa
     7741 ttccgatgcg atatccatct gattttcctg catcatatta atctgctcca tgtactggtc
     7801 gagcaattcg gaaatgctga tgtcgtaaat atctattttg tttttagaaa tcagcgtcag
     7861 caaaaggtca agagggcctt caaatgccga cagcttatat tgaagttgtt gtgtttccat
     7921 aaataagttt cagttatgat ttgatgttac gaatgatttt gaaatcaacc gacaaacggc
     7981 atatacggca ggcttgcaag ccagctgatg aagttaagaa tctgattgct gagccagtta
     8041 atcggagtgt tgagaattcc cacatacaag agaacaaaaa gaccgatgaa gaaatacatc
     8101 tgatatctgt aaaagaattc aacagccttg tcgggtaaaa atacgaacag aacctttgag
     8161 ccgtcaagag gcggaatcgg gataaggttg aacacagcaa gtccgacatt gcacgaaatg
     8221 tagaagctca agaattgaac aacatatcct ccgattgtcg aaagcggaaa tgcgcctgta
     8281 aaaaggataa tcggatagta aatgaggagt ccgacaaaag ccgcaacgat atttgcaatc
     8341 ggacccataa ttgcggtgat tgccataccg acctttggat ttttaaaatt tctcggatca
     8401 atcggaacag gctttgccca accgaatccg aacaaaagca tacacaaagc acccataggg
     8461 tcaatgtggc tgagcggatt aatcgaaagt ctgcctctgc cctttaccgc cttatcaccg
     8521 agaagtgacg cagtaaaagc gtgcgcccat tcatggaacg ggagaattaa aaatataata
     8581 acgagtacag ccaatatttc ggcaattacc gagccgaaat caaggttgcc gtttctgatg
     8641 ccgttaagta ataatgagag cataagagcc tcctgaaatc tgaattttta ggtaacaatc
     8701 gtgtaactat atttattcct aattatataa tatatcgtca aaaattacaa gagcaaaaaa
     8761 caagttaaga aattttcaaa aaacacttgc attttctctc attttctgtt attataacaa
     8821 agttagaaat agaattagtt ttctttaagg aggtgcagac gcatggcaaa atgtgaattc
     8881 tgcggtaagg atgtaaagtt cggtattaag gtatctcact cacacagaag atcaaacaga
     8941 acatggaagc ccaacgtaca gcgtgttaag gctattgttg acggttcacc aaagcgtgta
     9001 tatgcttgca cccgttgttt gcgttcaggt aaggttacca gagctaactg attttacaga
     9061 ttacgaaaca gctccactat ttacggtgga gctgtttttt tgtattttta tcagaatttt
     9121 aaacctcaat cacagcacag aaagagggct gacctttgtt tggtcagccc tcttaattat
     9181 tagtctttat cagccgttaa aggatttctt ccttcttacg aagaacgaac attacacaag
     9241 ttgagccgat gagaagtgta agaattacaa cagccagagg agcttcacct gtctgaacag
     9301 cagcatttgc attgttattg ttgccgttgt cagatgtatc cggagtgtcg gatgttgagc
     9361 tgattttctt tgttacaggc tgtgttacag atgttgcatc ctgtgaagtt acaggagcaa
     9421 ttgtatcctt agttgattcc tgcgttttct ctgtagcctt agtagcctca gtagatgact
     9481 ctgcggttga aggctcggct gttgtcggtt ctgcataggt tgaaggagta agctcagttg
     9541 aaagatcatt ttttactgcc gcagcaacag gagctttctt aactacttct ttatcaacaa
     9601 gaataacttc ttttgaaccg tcagtcattt ctgtgtccgg atttacttcc gaaacacgaa
     9661 gcacctcaac aacaaggtca accgtcgtcg aaaccggtgc aacctttgaa atgtctttaa
     9721 catcaaaaac aactgttgca aaaagcgtct tttcgctaaa gtcataaaga ccgagattac
     9781 ttgcaccgaa tcttacataa ccaacgcctt ccgacttatt cttgaagttt acatatacac
     9841 ctctgccgtc cattgtcggg caaaccgact tagcagtatt cacatcagca accgaaagga
     9901 ttgaagggtc ataagaaatg tgccactgcg tgtcgagcac attcttttga gactgcataa
     9961 aatatgtaac cgttacctga tttgtaactg cattatactt tgcacttgcc attgggaagt
    10021 aatttgatgt tgcatttact gtaagagcaa gcgaagattc agcagatgat gttgtaccct
    10081 ctgttgcagc agcaccatca gttgcagact cggttgtttc cgattctgtt acaacctctg
    10141 ttgttgattc ggctgttgtt tcctcacccg atggattgta ctctgtcggg aacacaccgt
    10201 ctttgccgta ataatcgccg gccttgaggt ctgccttttt aggagcattg ccgtattcac
    10261 cgttaccgta gtagtagaac cactcaccgg taaaaatcaa ttcatcctta tcgttctcgg
    10321 aagtaaattc aggattgata atataaatca tattgtcaaa gctttcaaca ccgtcgggat
    10381 aataatcaga ctcgccttta tcataaccct taaaattaat atcggttgtt cgataagcaa
    10441 gtttatattc aggatttgtc ggatcgtctg tacccttaag agcgttgttc cagattactt
    10501 tatcaacatc ggtcggtaca tcagaacagt aaacattttt gatatcagca ttgcgagctg
    10561 aataacccgg ccacgctgaa ggagtatccg aaccgttttc ccaataaaca cctgcatcag
    10621 atgtatattt gttttcccag tcatcaggct tacagaaata tatatggtag gtttcagtgc
    10681 cctcagaagg cacataactg ccttcatcgt ccgtatcggc agaaacgctt attgcagcaa
    10741 ccactatcat tgccgctgtg agcataacag ccaataaaag actgataact tttttggaca
    10801 tttgtatttc ctccttatga ttttccgaca ccatacttgc atctgtatgc ttattatagt
    10861 ataaaacgca gtaaaaatca agtgttacag ggaattttga gccttaatct caatgtaata
    10921 acaaccaaat ttttgtgtta aattccacca aatttgcatt ttacggaatt ttggcatatt
    10981 ttcaaccgta atttccgctt attcagtcat acataagaga acaaataata aacattaaag
    11041 cnnnnnnnnn ncttatgcga acaaaggcag tacaaacaac tgcaaatttt atctatgata
    11101 aaagcaaaaa tgccctccaa agcggagggc atcaaataat tatgtataat tatttaagat
    11161 tattcaaatc tcttgcgtgt gtaaagaact gcaccgcctg caacgaggag aactgcgaga
    11221 actgcagctg ctgttgtagc acctgtctga actgcgctgt tgtctgtgct ctttgctgtg
    11281 tcagcagttg aaactgcgtt tgcagaagat gttgggttct gacctgcagt aggaactgtg
    11341 ttctttgtag gatctgtgtt gttgttattt gacggagttg ttgttgtagg ctgatcaggt
    11401 gtagcaggtt ttgcattctt gtctactgtc tgaacaagac ctgcttcctt agccatatca
    11461 tagttaggaa gtgaaccgta ctcgtacttg cctgtttctt cgttaagacc atagtagaag
    11521 tagaactcac cggcatatgt ttccttacct gttacgctta cagaagtgtt ggtagggtct
    11581 ataacataaa tcatgttggt gaggtacatt gagatacccc agtcaggatc ctcaaagaag
    11641 ttgtcagcaa atttaccgag gaatgtcttg tcatcaccgt caaatgcttc ctgagccttt
    11701 gcccagaatt tgccgtcaag caaactgtca taatattcat tgtcgccttc cgaatagtac
    11761 tgacaagaaa catcggcagt ctgcttagca gccttaaact g
//
LOCUS       DAOMVE010000047        11635 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1307_length_11635_cov_2.920984, whole genome shotgun sequence.
ACCESSION   DAOMVE010000047 DAOMVE010000000
VERSION     DAOMVE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1307_length_11635_cov_2.920984"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..673)
                     /locus_tag="PLS64_04060"
     CDS             complement(107..673)
                     /locus_tag="PLS64_04060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HRL43039.1"
                     /translation="MNVYDFDKTIYYGDSTADFYLFCLKRHKKILTLAPSLLGAFLKF
                     YVFKKGTKTDFKEKMYRFLTYCDTEKDVNDFWKEYIGNIKPFYLEQKKDDDVIISASP
                     EFLLKPVCKRLKIKNLMASKVDMHSGKYSGVNCHGKEKVKRFYEAFPDGKIDNFYSDS
                     YSDSPLAEIAEKAFMVDGDKVEGWIFFK"
     gene            900..1763
                     /locus_tag="PLS64_04065"
     CDS             900..1763
                     /locus_tag="PLS64_04065"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HRL43040.1"
                     /translation="MFGYLQIQKSELLVRESEAYKAVYCGLCRQMGKDYSVFTRFTLS
                     YDCTFYAMLLMSLNRSCKGFKDGRCTCNPLKKCKFATDSGDAYRKAAAFSMISVYYKI
                     IDDIQDSGFFKKLLCRIIKPFFSHQRKKAADKYPDMDKAVSDMMKMQYDAEHSEKPSV
                     DMSAHPTALMLAAVLSAEAHDEIQKRVLYEFGYHIGRWIYLVDAADDIEKDIKSNGFN
                     PFVNKKTGEVKSSDFIKAVLNQSLARAYDAYNLMNFTDFKGILDNMMLLGFPASQNRV
                     TSKLDTEVNNE"
     gene            1756..2361
                     /locus_tag="PLS64_04070"
     CDS             1756..2361
                     /locus_tag="PLS64_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRL43041.1"
                     /translation="MNNPYEVLGVSETATDEEVKAAYRNLAKKYHPDNYTDSPLADVA
                     EQKMKEINEAYDMINQMRQKGKGTGSTGSSSYNANSSSSQFYNVRIYIQRGLLDQADQ
                     ILENTAQSMRTAEWHYLKGMIAYRRGWSEQAFSYFTTACNMDPNNTEYRAALNNMQSQ
                     RAYNYGGYTQTNPNATGSSFCDVCAGMMCANCLCDCCRMGC"
     gene            2363..2920
                     /locus_tag="PLS64_04075"
     CDS             2363..2920
                     /locus_tag="PLS64_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43042.1"
                     /translation="MKKNGIKKGTMRIAVCGIIAALSLVFMMMTSLVPIGTYALPCLA
                     GILISCIVVEYGYGWAIGVFCVTAVLSTLLAGDKEAVIYFIALFGYYPILKGAFEFKI
                     KNRAVQYILKFAVFNVAAIGSFFVATWLLSIPTDEFTVFGFYVPWAFLIAGNIFFLLY
                     DYAITVFVMQYVRRLRGKIFGNFHK"
     gene            2957..5410
                     /locus_tag="PLS64_04080"
     CDS             2957..5410
                     /locus_tag="PLS64_04080"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRL43043.1"
                     /translation="MKKQKLFVRIVCIVLVVLMAVSVGAVAITAFSANAAQSTEEVSA
                     SVSAGASGYINASYVNLRSGAGTNYSVITCMAKNTKFTFVDGKLYNSKWYKIKLKSNS
                     KTGYVTKEYVAVSSSATASSVTGYVSDDYVNLRSGAGTNYSVVGCLRKNTKLTFVSTS
                     LYNSAWYKVKVTSTAKTGYIKKDYVKMNSSTQPTTKATQPTTKATKPVTQPTASASTV
                     SGYITDDYVNLRSGAGTSYSVVECMRVNTKVTFVSTKLYNNDWYNIKLTSNSKTGYVK
                     KDYVKMNGQTQPTTTRPTTTKPSTGSSVKLSVSSKSIFTGNRFAITATASGSVSWSSS
                     NTGVATVDSRGIVTAKKAGSATITAKSGSHSATCKITVKSGSSVNISNSNVNLPWQKS
                     MLLKSSTSGVTWSSSNTKIATVKNGVVDTVGKGYVTITASTSYGAATCLIHVMPRESV
                     RFCYASPNSAPLNSNVSFKAITDTGRVGVYFVVTNGSTSYKVTAKNKVKDGNSYIWTG
                     TQKLSKSGKWSVKAYSKFKTESKYYTTAGGGEGEVFVTSTTNKTTTACAERRASDEVI
                     KLIANYEGFLSKVTADSITTDPTLGYGKVVISGEQFYNNITSNQAYAYLCQTVNKGGY
                     TTTTNSYLVNNGIKFNQRQFDALVCFAYNVGSGVFYNDSELQSVLLNTGSSGTIKAGA
                     SGTVTGSDVNLRRGAGTNYSVVTRMNYGTKLKFVDGKRYNTNWYKVKLSNGTTGYIHK
                     DYVSASGGSRDLNNVNKQNLIDALLQYHHAAGSCYWGLLYRRVDEAETFLYGDYDRDG
                     QHNYHHFHFSCYSNPSFGI"
     gene            5439..6239
                     /gene="proC"
                     /locus_tag="PLS64_04085"
     CDS             5439..6239
                     /gene="proC"
                     /locus_tag="PLS64_04085"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRL43044.1"
                     /translation="MKKIGFIGAGNMATAIIKGLMAQNDGKADFINVFDVSEEKCAAM
                     KNMGANVMTSADEIAKNSSIVVLAVKPQNYPEVLESLKNSITTAKTVVSIAAGISIAY
                     VRKGLECDCPVVRVMPNTPLLLKKGATALCPSENISDDDKTIVYNMFAGSGVCEYIDE
                     SHMNEIIAVNGSSPAYIYLFAKAMADYAKNCGIDYDKAMNLVCATLEGSAAMLRDSGE
                     PVETLIDRVCSKGGTTIAAMDKLKEHGFYEAVLDGMDACTKRAEELGK"
     gene            6311..6979
                     /locus_tag="PLS64_04090"
     CDS             6311..6979
                     /locus_tag="PLS64_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HRL43045.1"
                     /translation="MKSIVFSFKKRYLFRRSKGISPSDVRFFIRRYSVVLIFVSAFLT
                     GLVFGSIYASKADKQMLDSLDFLFTTNLDARLGQNAVSTFCACFASDFIFLTVVFLLG
                     LAPWGIPFLPFVSAFKGFGTGLTAAYLIITYSLKGAGFYLLVVLPGTFLFCLALVKLS
                     AYAFEISKQMFFSLIGHSHQTNSLKNEVIDFCSRSVSALIMTFCSTLLDTALWCLFSG
                     AFNF"
     gene            6994..7413
                     /locus_tag="PLS64_04095"
     CDS             6994..7413
                     /locus_tag="PLS64_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HRL43046.1"
                     /translation="MASEKNAVALTGFFSSLFHNLPRLLLTNVLFALPFAVIFGIFFL
                     INTLTGLNSMFIYFLTIIPLFPFYAGVTQVTSHMVRGEENVDVFSNFIGGIKENLLRF
                     LIHGVVMYAAVFISYYSIVLYLGLGSKNGMFYVPLVF"
     gene            7434..7937
                     /locus_tag="PLS64_04100"
     CDS             7434..7937
                     /locus_tag="PLS64_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43047.1"
                     /translation="MFFYVPPMTVTFDIKMKDIYKNSALMTIGELKHNLFAVFGILIL
                     FLVCATVLMCSFTPVLLIIFTIVLALFIVPSILSFIINSAVYKNMYSMIVDKDSKSKT
                     IDKKMENRRKGQFRDDEEPVAEDYSDLEIDESADGDEFIFYNGKMMKRSYLLKLKKEA
                     EERKNAK"
     gene            7934..8584
                     /locus_tag="PLS64_04105"
     CDS             7934..8584
                     /locus_tag="PLS64_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43048.1"
                     /translation="MSGKHAVKSSKKSLIIIICVVVLVAVIAGAVILFMNLSGKNNNS
                     ATTVPTVAASTTADNTNSTQNVSDSVSETTQPATKSGEASQEPTEQGYTAETSSNDSE
                     IVVPTISGEKQKPKFSATLIPYYAKDENSSEEYSLRDLFGSAYSGGGFTFNEDGTFID
                     GITSASANSGAYIVEGDSVVITYSNDKNVIAAVTKWNGDVPAEITVNYGGIIVSFK"
     gene            8600..9106
                     /locus_tag="PLS64_04110"
     CDS             8600..9106
                     /locus_tag="PLS64_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43049.1"
                     /translation="MSKKSVKKPDYAKFEANRAVKKKNKKKKTTAIVSSVIAACVFII
                     AAIVAVNVLSPSVTAELTSSAWVPEGAKNASGDEVEMSEVYNTNYSAYQGSMSFSDDG
                     TFTLWLSPGSPDDGTHSGKYTVSASDKVNLSFDDGTNTSAALTQKNGKISAVVLKYND
                     YEITFVKQ"
     gene            complement(9165..10037)
                     /locus_tag="PLS64_04115"
     CDS             complement(9165..10037)
                     /locus_tag="PLS64_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRL43050.1"
                     /translation="MHKVKAKGILSAANGMNIYRGCQHGCIYCDARSKCYQMNHRFED
                     IEVKENAPELLENALKRKRRKCMIGTGAMSDPYIPLEKELKLTRRCLEIINRYGFGVT
                     VQTKSASVMRDIDLFTKINDKSKAVLQMTLTTADESLCRIIEPNVSVTKERFETLKAF
                     HENGIPSVVWFSPFLPFINDTKENIDGLLKYCIDAKVRGIICFGIGLTLRDGDREYFY
                     SRLDKHFPNMKERYIYTYGNSYQLTSGNNNVLMNRISEVCRNHGIMFGVENVFSYLHK
                     FESKTEQLSLFDGI"
     gene            complement(10050..10283)
                     /locus_tag="PLS64_04120"
     CDS             complement(10050..10283)
                     /locus_tag="PLS64_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43051.1"
                     /translation="MKKYVKVTATFDCDGDLIPEYIVWDDGRRYRIDKISDVRYAASL
                     KAGGAGIRYTCHILGKERYLFLEENRWFVDAKQ"
     gene            complement(10348..11622)
                     /gene="dinB"
                     /locus_tag="PLS64_04125"
     CDS             complement(10348..11622)
                     /gene="dinB"
                     /locus_tag="PLS64_04125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRL43052.1"
                     /translation="MNINCAVDKSKYKKDRVILHSDCNGFYASVECLHHPEIRNKPVA
                     VSGDAENRHGIILAKNEIAKKYNIKTGEAIWQAKQKCPDLITVPPHFDLYKRFSKMAR
                     RIYSEYTDKIEPFGLDEAWLDVTDNKNMNGKEIALEINRRIKQELGITVSIGVSFNKI
                     FAKFGSDYKKPDAITEITRENYRDIVWNCPAKDLLYVGRATGRKLESIGIYTIGDIAT
                     ADVTLLRSYLGKWGDLIYGFANGYDSSPVAHMNENSEVKSIGNSTTTPKDMVTFNDVK
                     QVIFVLCDSVCRRMREQGFMAKTVCITIRDNELMSFNRQHTTEIHTNLTKDITNTAIE
                     LFKKSYKMEKPLRSIGVSVTDFIHDDQPHQMSLLRDEKRLIQNEQLDKTLDRLKKRFG
                     NYAVRPAVLLDDKELSDFNPKEDHTIHPVGYL"
BASE COUNT         3415 a         2325 c         2343 g         3552 t
ORIGIN      
        1 ttcaacgggt aaaaccgttg aaacgtttgc ccgaaacaaa cacggctcaa tgttttggca
       61 acccgaaaaa aataaaaata cggaacagat ttctgctccg taaaaattat ttaaagaata
      121 tccaaccttc aaccttgtcg ccgtcaacca taaaagcttt ttctgcaatt tccgcaagcg
      181 gcgaatcact gtaactgtcc gaataaaaat tgtcaatttt tccgtcagga aaagcctcat
      241 aaaatcgttt taccttttcc ttgccgtgac agtttacacc cgaatatttt ccgctgtgca
      301 tatctacctt tgacgccata aggtttttta ttttgagcct tttgcaaacg ggtttcagca
      361 aaaattcggg tgatgccgaa ataattacat catcatcttt cttttgctca aggtaaaacg
      421 gttttatatt tcctatgtat tccttccaga aatcatttac atccttttca gtatcgcaat
      481 aggtaagaaa tctgtacatt ttttctttaa aatcagtttt tgtacctttt ttgaatacat
      541 agaattttaa aaatgctccc aaaagcgatg gggcaagagt gagtattttt ttatgccttt
      601 taaggcagaa taaatagaag tcggctgttg agtccccgta gtaaatcgtt ttgtcaaaat
      661 cgtaaacatt cataatatgc aaattccttt ttagttttgc aaataatttt tgacattcaa
      721 ttcttaacag aagagaacat ctgcactatg attataatat gagaagaaaa tttttgcaac
      781 atttcccgtt aataagctca ttttggctga ttatccaaag aatataccgt tattctgttg
      841 cttttaactc tgttttgttg tacaatatac tatgtattat tgtataaagg agaaaatccg
      901 tgtttggtta tttgcagatt caaaagagcg aactgcttgt tcgtgaatcc gaggcttata
      961 aggctgtgta ttgcggtctt tgcaggcaaa tgggtaagga ttactccgtg tttacaagat
     1021 ttacgctaag ctatgactgc acattttatg caatgctttt aatgtcgctt aaccgttcat
     1081 gcaaaggctt taaagacggc agatgcacct gtaatcctct taaaaaatgc aagtttgcaa
     1141 cagattccgg tgacgcatac agaaaggctg ccgcattttc aatgatatcg gtttattata
     1201 aaatcattga tgatattcag gacagcggat tttttaaaaa gcttttgtgc agaatcataa
     1261 agcctttctt ttctcatcag aggaaaaagg cggcagataa gtatcccgac atggacaaag
     1321 cagtttctga tatgatgaaa atgcagtatg atgctgaaca ttcggaaaag ccgtctgtag
     1381 atatgtcggc tcatccaacg gcattaatgc tcgctgctgt attgtcggct gaggctcatg
     1441 atgaaattca aaaaagagtt ctctatgaat tcggctatca tatcggcagg tggatttatc
     1501 ttgtcgatgc cgcagatgat atagaaaaag atatcaaatc aaacggattt aatccgtttg
     1561 taaacaagaa aacaggtgag gtaaaatcat cggattttat aaaagcggtg ctgaatcagt
     1621 cacttgcgag ggcatatgat gcctacaatc ttatgaattt taccgatttt aagggaattt
     1681 tagataatat gatgcttttg ggatttcctg catcacaaaa cagagtaaca agtaaacttg
     1741 atacggaggt caataatgaa taatccttat gaggttctcg gtgtgtctga aacagctacc
     1801 gatgaagaag taaaagcagc gtacagaaac cttgctaaaa aatatcatcc cgataactac
     1861 accgacagcc ctcttgcgga tgttgcggag cagaaaatga aggagattaa tgaggcatac
     1921 gatatgatta atcagatgcg tcagaagggt aagggtacag gctcaacagg cagttcgtcc
     1981 tataacgcaa attcttcaag ctcacagttc tacaatgtaa gaatttacat tcagagagga
     2041 cttcttgatc aggccgatca gattcttgaa aacacagcac agtcaatgag aaccgccgaa
     2101 tggcattatc tcaagggcat gattgcatac cgcagaggtt ggtctgaaca ggctttttca
     2161 tatttcacaa ctgcgtgcaa tatggacccc aacaacacgg aataccgagc cgctcttaac
     2221 aatatgcaga gccagagagc atacaactac ggcggttata ctcaaacaaa tcctaacgca
     2281 acaggctcat ccttctgcga tgtttgtgca ggaatgatgt gtgcaaactg cttgtgtgat
     2341 tgttgcagaa tgggatgtta atatgaagaa aaacggtatc aaaaagggga caatgcgtat
     2401 tgcggtttgc ggaattattg cggcgctttc actcgttttt atgatgatga catctctcgt
     2461 tccaatcgga acatacgctt tgccctgtct tgcaggaatt ctcatttcct gcatagttgt
     2521 tgaatacggt tatggatggg caatcggtgt gttttgtgta actgccgtgc tttcaacgct
     2581 ccttgccggc gataaagagg ctgttatata tttcattgca cttttcggct attatcccat
     2641 tttaaagggc gcttttgaat ttaaaatcaa aaacagggca gttcaataca tactcaagtt
     2701 tgcggtattt aatgttgccg ccatcggttc gttctttgtc gcaacatggc tgttgtcaat
     2761 tccgacagat gaatttaccg ttttcggttt ttatgttccg tgggcatttt tgattgccgg
     2821 aaatattttc tttttgctct atgattatgc aattaccgtc tttgttatgc agtatgttcg
     2881 cagattaaga ggaaaaatat tcggtaattt tcacaagtaa ttataattca aagaagtttt
     2941 gagaaggtgg acctaaatga aaaagcaaaa attgtttgtc cgcatagtct gcatagtcct
     3001 tgtggttctt atggcagtat cggtaggtgc tgttgcaata actgcatttt cggctaatgc
     3061 ggctcaatca accgaggagg tttccgcaag cgtatcagca ggcgcatcag gctatataaa
     3121 tgcgtcgtat gtaaatctga gaagcggtgc aggcacgaat tattcggtta taacctgtat
     3181 ggcaaagaac acaaaattta cttttgttga cggaaagctc tataactcaa agtggtacaa
     3241 aatcaagctt aaatcaaatt caaagacagg ctatgttaca aaggaatatg ttgccgtaag
     3301 ttcttcggca acggcatcaa gcgtaacagg ttatgtttct gacgattatg tgaatctcag
     3361 aagcggagca ggcacaaatt attctgttgt tggttgcctg agaaagaaca ctaagcttac
     3421 ttttgtaagc acttcactct acaattccgc ttggtataag gttaaggtta cttcaactgc
     3481 taaaacaggt tacataaaaa aagactatgt aaaaatgaac agttcaaccc aacctacaac
     3541 gaaggcaaca caacctacaa caaaggcaac caaacctgta actcagccga ctgcgtcagc
     3601 gtcaaccgta agcggttata taacagacga ttatgtaaat ctcagaagcg gtgccggtac
     3661 aagctattcg gttgttgagt gtatgagagt aaatacaaag gttacttttg taagcacaaa
     3721 gctttataac aatgattggt ataatattaa gcttacatca aattcaaaaa caggctatgt
     3781 aaaaaaagac tatgttaaga tgaacggtca gacacagcct acaacgactc ggcctacaac
     3841 cacaaaaccg tcaacaggtt catctgttaa actttccgta tcaagcaaat caattttcac
     3901 aggcaacaga tttgcaatta ctgcaacagc gtcgggaagt gtttcatggt caagctcaaa
     3961 tacaggcgtt gcaaccgttg actctcgtgg tattgtaaca gccaagaaag caggctctgc
     4021 aacaattact gcgaaaagcg gttcgcattc tgcaacctgc aaaattaccg taaaatccgg
     4081 ctcatctgta aatatatcca acagcaatgt aaatcttccg tggcagaagt caatgctttt
     4141 gaaatcaagc acaagcggtg tcacatggtc aagttcaaat acgaaaatcg caacagttaa
     4201 gaacggtgtt gttgatacgg taggcaaggg ctatgtaaca atcactgcct caacatcata
     4261 cggtgcggca acctgtctta tccatgttat gccgagggaa agcgtgcgtt tctgctatgc
     4321 gtcaccaaac tccgctccgc ttaattcaaa tgtaagcttt aaggctatta ccgacacagg
     4381 cagggtaggc gtgtattttg tagttacaaa cggttcaacc tcttataagg tcaccgcaaa
     4441 aaataaggtt aaagacggta actcctatat atggacaggt acacaaaagc tttcaaaatc
     4501 aggcaaatgg tctgttaagg catattctaa atttaaaacc gaatcaaagt attacaccac
     4561 ggcaggcggc ggtgagggcg aggtgtttgt aacctcaaca accaataaaa ccaccacagc
     4621 ctgtgccgag cgcagagcga gtgatgaggt aatcaagctt attgcaaact atgagggatt
     4681 tctctcgaaa gtcacagcag acagcattac aaccgatcct acactcggtt acggcaaggt
     4741 tgtaatttca ggcgaacagt tctacaacaa cataacttca aatcaggcat atgcttatct
     4801 ttgccagacc gtgaacaagg gcggatatac caccacaaca aattcctacc ttgtaaacaa
     4861 cggcataaag ttcaatcagc gacagtttga cgcacttgtg tgctttgcat acaatgtagg
     4921 cagcggtgtg ttctacaatg attccgaact tcagtctgtt ctcttgaaca caggttcaag
     4981 cggtactatc aaagcaggcg cttcgggtac ggttacaggt tcagatgtaa acctcagaag
     5041 aggtgcagga actaactact ccgttgttac ccgtatgaac tacggaacaa aacttaaatt
     5101 tgttgacggc aagcgttata acacaaactg gtataaagtt aagctttcaa acggcacaac
     5161 aggatatatt cacaaggact atgtttctgc ttcgggcggt tcaagagatt tgaataatgt
     5221 caataagcag aaccttattg atgcactttt gcagtaccac catgcggcag gttcttgcta
     5281 ttggggactt ttgtacagaa gagttgacga ggcggaaacc ttcctctacg gcgattatga
     5341 ccgtgacggt cagcacaatt atcaccactt ccatttctca tgttactcaa acccgagttt
     5401 cggtatctga tattaatttt tgcaaagaag gtcttaaaat gaaaaaaata ggtttcatag
     5461 gcgcaggcaa tatggcaaca gccattataa aaggtcttat ggcacaaaac gacggcaagg
     5521 ctgattttat caatgttttt gatgtaagcg aagaaaagtg tgccgctatg aaaaatatgg
     5581 gtgcaaatgt aatgacatct gccgatgaaa ttgcaaagaa cagctcaatt gttgttcttg
     5641 cagtcaagcc gcagaattat cccgaagttc ttgagtccct caaaaacagc attacaacag
     5701 ccaaaaccgt agtatcaatt gccgcaggca tatctattgc atatgtaaga aaaggtcttg
     5761 agtgtgactg ccctgttgta agggttatgc ctaatacgcc tctcttgctc aaaaagggtg
     5821 caacagccct ttgtccgtct gagaatatca gcgatgatga caaaacaatc gtttacaata
     5881 tgtttgcagg cagcggtgtt tgcgaatata ttgacgaaag ccatatgaat gaaataattg
     5941 cggtaaacgg cagcagtcct gcttatattt atctttttgc aaaggctatg gctgattacg
     6001 caaagaattg cggaatcgac tatgacaagg ccatgaacct tgtctgtgca actcttgagg
     6061 gttcggcagc aatgctccgt gacagcggtg agcctgttga aacactcata gacagggttt
     6121 gctcaaaggg aggcacaaca attgccgcta tggataagct taaagaacac ggcttctatg
     6181 aggctgttct tgacggcatg gacgcttgta caaagagggc agaggagctt ggcaaataat
     6241 tttgccttaa cataaaatga atacattcct catatatttt aacggacaac caatgagagg
     6301 aatgtatttt atgaaaagca ttgtattttc ttttaaaaag cgttatcttt ttcgcagatc
     6361 aaagggaatt tcaccgtctg atgtcagatt ttttataagg cgatattccg tggtgcttat
     6421 ctttgtttcg gcttttctta ccggacttgt tttcggttca atttatgcat caaaagccga
     6481 taagcaaatg cttgattccc ttgattttct gtttactaca aatcttgatg caaggctcgg
     6541 tcagaatgcc gtcagcacat tttgtgcctg ctttgcatct gattttatat ttctcacggt
     6601 tgtattcctc ttgggacttg caccgtgggg aataccgttt ttgccgtttg tttcagcatt
     6661 taaaggcttt ggaacaggac ttactgcggc atatcttatt attacatatt cacttaaagg
     6721 agcaggtttt tatctgcttg ttgtgttgcc gggaacattt ttgttctgtc tggcacttgt
     6781 aaagctgtcg gcatatgctt ttgaaatttc aaaacagatg tttttcagct taatcggtca
     6841 ttctcatcaa acaaattctt tgaaaaatga ggtgattgat ttttgttccc gatctgtttc
     6901 ggcactcata atgacctttt gttcaacctt gctggacacg gctttgtggt gcttgttttc
     6961 gggagcgttc aatttttaat tttggagggc gctatggcat cggaaaagaa cgctgtagca
     7021 ttgacaggat ttttctcgtc attgtttcac aatttaccac gacttttgct aacgaatgtg
     7081 ctttttgcat tgccgtttgc cgtaatattc ggaattttct ttttgattaa tacccttacg
     7141 ggtcttaatt caatgtttat atattttctt acgataattc cgctttttcc gttttatgcc
     7201 ggcgtaactc aggttacttc ccatatggta aggggagagg aaaatgttga tgttttctca
     7261 aatttcatcg gcggaataaa ggaaaatctg ctcagattcc tcatacacgg cgttgtgatg
     7321 tatgccgctg tttttatcag ctactactca attgtgcttt atctcggact cggttcaaag
     7381 aacggaatgt tttatgttcc gcttgtattt taattgcaat tttctttctg tttatgttct
     7441 tctatgttcc gcctatgacg gttacttttg atatcaagat gaaggatatt tacaaaaaca
     7501 gcgcacttat gacaatcggt gagcttaaac acaatctgtt tgcagttttc ggaattctta
     7561 ttttattcct cgtttgtgca acagtgctta tgtgcagttt tactccggta ctgctcatca
     7621 ttttcacaat tgttcttgca ttgttcattg ttccgtcaat tttatctttt ataatcaatt
     7681 ctgcagttta taagaatatg tattcaatga ttgttgacaa ggattcaaag agcaaaacaa
     7741 ttgataagaa aatggagaac agacgcaaag gtcagttccg tgatgatgaa gagcctgttg
     7801 cagaagatta ttcggatctt gaaattgatg aatcggctga cggtgatgaa tttattttct
     7861 ataacggaaa aatgatgaaa agaagttatc ttttaaagct taaaaaagag gctgaagaaa
     7921 ggaagaacgc aaaatgagcg gtaaacacgc agttaaaagt tcaaaaaaat cattgataat
     7981 aattatttgt gttgttgtac ttgttgcagt aattgcaggt gcggttatcc tgtttatgaa
     8041 tctttcgggt aaaaataata attcggcaac aacagtaccg actgtggctg cttcaacaac
     8101 cgccgataat acaaactcaa cacagaatgt ttccgactct gtatccgaaa ccacacagcc
     8161 tgcaaccaaa tcgggtgaag cttcacaaga gccgacagaa cagggataca cagccgaaac
     8221 atcttcaaat gacagtgaaa ttgttgttcc gacaatcagc ggagagaagc aaaagccaaa
     8281 attcagtgca actcttattc cgtactatgc aaaagatgaa aattcttctg aagaatattc
     8341 tttgagagat cttttcggct ctgcttattc aggcggcgga tttactttta atgaggacgg
     8401 aacattcatt gacggaatca caagtgcctc ggcaaattcg ggtgcgtaca tagttgaggg
     8461 cgactccgtt gtaataacat attccaacga taaaaatgtt attgctgccg taaccaaatg
     8521 gaacggtgat gttcctgctg aaattaccgt aaactacggc ggaattattg tatcctttaa
     8581 gtaaaatatc ggtgattaaa tgagtaaaaa atctgttaaa aagcctgact atgcaaaatt
     8641 tgaggctaac agggctgtaa aaaagaagaa taagaaaaag aaaacaacag caatcgtttc
     8701 atcggttatt gccgcctgcg tatttatcat tgcggcaatc gttgccgtta atgtgctttc
     8761 accgtcagta accgccgagc ttacctcatc tgcatgggtt cccgaaggtg ctaagaatgc
     8821 gagcggtgat gaggttgaaa tgagtgaggt ttacaacaca aattacagtg cttatcaggg
     8881 ttctatgtca ttttccgatg acggcacttt tactttatgg ctttcgccgg gaagtccgga
     8941 tgacggaact cacagcggaa aatataccgt gtcagcttcg gacaaagtca atttgtcatt
     9001 tgatgacgga acaaatactt ccgccgcact cacacaaaag aacggtaaaa tttcggctgt
     9061 tgtgcttaaa tataacgact atgaaatcac atttgtaaaa cagtgaatac ttaaaacaaa
     9121 aagatatacg gtaaaatttt ctgccgtata tcttttttat ttttctatat tccgtcaaac
     9181 agacttagct gttcggtttt gctttcaaac ttatgcaaat acgaaaacac attttcaact
     9241 ccgaacataa ttccgtgatt tctgcaaact tctgaaatcc tgttcatcag aacattattg
     9301 ttaccgctcg taagctgata tgaatttccg tatgtataaa tgtatcgttc tttcatattc
     9361 ggaaagtgtt tgtcaagccg gctgtaaaaa tattctctgt caccgtcacg cagagtaaga
     9421 cctatgccaa agcatattat tccacgaacc tttgcgtcaa tgcaatactt taaaagtccg
     9481 tcaatatttt ctttcgtatc atttataaac ggtaaaaacg gagaaaacca cacaacactc
     9541 ggaataccgt tttcgtgaaa tgccttgagc gtttcaaatc tttctttagt aacggaaaca
     9601 ttcggttcaa ttattctgca aaggctttcg tctgccgttg taagcgtcat ctgtaacaca
     9661 gccttgcttt tatcgtttat tttcgtaaac aggtcaatat ctctcataac agatgccgac
     9721 tttgtctgta cagttacacc aaatccgtag cggttgatta tttcaagaca tcttcgtgtc
     9781 agtttcagtt ctttttcaag aggaatgtac ggatcgctca tcgcacccgt accaatcata
     9841 cattttctgc gtttcctttt taaagcattt tcaagcaatt ccggggcatt ttctttcacc
     9901 tcaatatctt caaatcggtg attcatctga tagcatttac tgcgtgcgtc acaataaatg
     9961 catccgtgtt gacatcctct gtaaatgttc atgccgtttg cggcagataa aattcccttt
    10021 gctttgacct tatgcatata atcctccgct tattgtttgg catcgacaaa ccacctgttt
    10081 tcctccaaaa acaggtatcg ttctttgccg agaatatggc aggtgtagcg tattcccgca
    10141 ccgcctgctt tgagagatgc cgcataccgc acatcggaaa ttttgtcaat gcggtatctc
    10201 cttccgtcat cccacacaat atattcgggg ataagatctc cgtcacaatc aaatgtggca
    10261 gtaactttaa catacttttt catagcttgt tacctccatt aaaatcagag aaacatcttt
    10321 aatttttttg cctaagatgc atcgttatca taaatatccg acaggatgaa ttgtatgatc
    10381 ctcctttggg ttaaaatccg aaagctcctt gtcatcgagc aaaacggcag ggcgaacagc
    10441 ataattgccg aatcgctttt ttaatctgtc aagtgttttg tcaagctgtt cgttctgaat
    10501 aagtcgcttt tcatcacgca aaaggctcat ctggtgcggc tgatcatcgt gaataaaatc
    10561 cgtaacgctt accccgatac ttctcagcgg cttttccatt ttatagcttt ttttgaaaag
    10621 ctcaatagcc gtatttgtta tatcctttgt aagatttgtg tggatttccg ttgtgtgctg
    10681 tcggttaaat gacatcagct cgttgtcacg aatcgtaatg caaaccgttt ttgccataaa
    10741 tccctgttca cgcattcttc tgcaaacgct gtcacacaaa acgaatataa cctgttttac
    10801 atcgttaaag gttaccatat ctttcggagt tgtggttgag ttaccgattg actttacctc
    10861 gctgttttcg ttcatatggg caacagggga agagtcgtat ccgtttgcaa agccgtaaat
    10921 taggtcgccc cattttccaa gatatgaccg caacagtgta acatcggcag ttgcaatatc
    10981 tccaatcgta taaatgccga tagattcaag ctttctgccc gttgctctgc caacatacaa
    11041 aaggtctttt gcaggacagt tccacacaat atctctgtaa ttttctcgtg tgatttccgt
    11101 tatagcgtca ggctttttat agtcacttcc gaatttggca aaaattttat taaagctgac
    11161 tccgatgctt acggttattc cgagttcctg ctttatacgg cgattaattt cgagtgcaat
    11221 ctctttgccg ttcatattct tattgtcggt aacatcaagc catgcctcgt caagtccgaa
    11281 cggctcgatt ttgtcggtgt attcgctgta aattctcctc gccatttttg aaaatcgctt
    11341 gtacaaatca aagtggggag gaacggttat gaggtcggga catttctgct ttgcctgcca
    11401 gattgcctcg cctgttttta tattatactt cttcgcaatc tcatttttag caagaataat
    11461 tccgtgccga ttttccgcat caccgctcac ggcaacaggt ttgttcctga tttcgggatg
    11521 atgcaaacac tcaacgctcg cataaaatcc gttgcagtcg ctgtgaagaa taaccctgtc
    11581 ttttttatat ttgcttttgt caacggcaca attaatattc acaacgcatc acctc
//
LOCUS       DAOMVE010000048        11614 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1310_length_11614_cov_3.221732, whole genome shotgun sequence.
ACCESSION   DAOMVE010000048 DAOMVE010000000
VERSION     DAOMVE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1310_length_11614_cov_3.221732"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PLS64_04130"
     CDS             complement(<1..1488)
                     /locus_tag="PLS64_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43053.1"
                     /translation="MFKKALSLLLSMMLVVTSLVATVVSVSAAGDSYFVSGCEELTGY
                     KWAETEATCGDNVMTENGDGNYEKVFTNVAVGNGYQFKIVKNDTEWIGVGDTGNDNFT
                     FNVTKECDVTVTYNPTTNEITATGEGVVIPKELVVDHITVVGNGEESWLNGKDWKVDA
                     EANYMTETSEGSKVYQIKFESLDAYENYQFKFAANGSWADNWGLPEQGTAPLNEWFDL
                     TYNGQNMIIDTDAAGYEDGYDIVLTLDLSNFNYATKQGAKGKVDIVTGAEPTTVAEPT
                     TVAEPTTVAEPTTVAEPTTAAPAKKGLTVTATSNLFSEKQVTLSADQKTVRVTYMFQT
                     KDRDGMLNFQYKMNYDPAVLKVSDNTRKSTSFEYPNFEFNKNVAPGLLMGIGSDTSLY
                     FSSEKEIVFSSVEFDVINPEAVATTVDLDVEYLTISNQDPVSELEIPSDEVVLLDKSV
                     LNQDAFDKYVAVAKTVTVPDGSEEPTTVEPTTVEPTTVAEPTTVEP"
     gene            complement(1643..2656)
                     /gene="galE"
                     /locus_tag="PLS64_04135"
     CDS             complement(1643..2656)
                     /gene="galE"
                     /locus_tag="PLS64_04135"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRL43054.1"
                     /translation="MNVLLAGGAGYIGSHTCVELINAGHDVVIADNFSNSCPVAVERV
                     EELTGRKIPLYEADVCDRDAVEKIFSENKIDAVIHFAGLKAVGESCEKPVEYYRNNID
                     STLTLLEVMKKHGVNNFIFSSSATVYGTPETVPLVETMPKGSPTNPYGWTKFMMEQIL
                     TDTANANPDMSVVLLRYFNPIGAHESGRIGEDPNGIPNNLMPYITQVAAGRLKCLGVF
                     GNDYPTHDGTGVRDYIHVVDLAKGHVKAIEYSAEHKGTEIFNLGTGVGYSVLDIVKAF
                     EKANSIEIPYVIKPRRAGDIAECFADATKAKKILGWIAEKSLEDMCRDSWNWQSHNVN
                     GYK"
     gene            complement(2679..3197)
                     /locus_tag="PLS64_04140"
     CDS             complement(2679..3197)
                     /locus_tag="PLS64_04140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL43055.1"
                     /translation="MSNPIVTFEMENGKTIKAELYPEIAPNTVNNFLSLVNKGYYDGL
                     TFHRVIYGFMIQGGCPEGTGMGGPGYSIKGEFAVNGFENNLKHTEGVLSMARSMMPDS
                     AGSQFFIMHKNAPHLDGQYAAFGKVIEGMDVVNEIAECDTDYSDKPLDPQIMAKVTAE
                     TFGVDYPEPEKC"
     gene            complement(3284..4294)
                     /gene="tsaD"
                     /locus_tag="PLS64_04145"
     CDS             complement(3284..4294)
                     /gene="tsaD"
                     /locus_tag="PLS64_04145"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRL43056.1"
                     /translation="MKILAIESSCDETAAAVVEDGRNIISSVVASQVEEHKLYGGVVP
                     EIASRRHAEAIVPVVRQALEDADCRLSDIDAIGVTYAPGLIGALLVGVNFAKGLSLAT
                     GIPLVPVHHLRSHIASNYISNKELKPPFLCLVVSGGHSHIVMVEDYTKMKIIGKTRDD
                     AAGEAFDKAARTMGMPYPGGIEMDRVAENGNPFAFKLPRPAVHDAPYDFSFSGLKTAV
                     INLIHNSAQKGVELNKADVCASFRYAVVDCLVTNFIKAAEDLGQKKLVIAGGVSANSL
                     LRRTLAEECKKRDWEMYMPEKSLCGDNAAMVGSQGYYEFLSGNIAGTDLNAFATMSIE
                     KG"
     gene            complement(4372..4806)
                     /gene="rimI"
                     /locus_tag="PLS64_04150"
     CDS             complement(4372..4806)
                     /gene="rimI"
                     /locus_tag="PLS64_04150"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRL43057.1"
                     /translation="MKIDRMTENQLEAVAQLEQRCFSHPWSFNSLEEELNNETSLFFT
                     ATEDNAVVGYIGMSVVVDEGYIFNVAVDGTHRNRGIGSALVETLVTYAKKNNLCFLTL
                     EVRESNGNARSLYEKFGFIKVGERKNYYSSPTENAVLMTRCF"
     gene            complement(4796..5233)
                     /locus_tag="PLS64_04155"
     CDS             complement(4796..5233)
                     /locus_tag="PLS64_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HRL43058.1"
                     /translation="MMQKDNRTIAFVCTGNTCRSPMAEAIFNDMAEKNGVCVRAESFG
                     IATSTGLPVSENSVIACKEIGIDLSGKASTAVADAGIEKYEKFYCMSQSHAKMLMQFF
                     LVQPEKIAVLGISDPYGGDIEVYRHCRDEIVNSVKEILKAYEN"
     gene            complement(5217..7781)
                     /gene="polA"
                     /locus_tag="PLS64_04160"
     CDS             complement(5217..7781)
                     /gene="polA"
                     /locus_tag="PLS64_04160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRL43059.1"
                     /translation="MRLLAVDGNSIVNRAFYGIRPLTTKDGQFTNAIYGFLTMLYKIK
                     NEENPDAVAIAFDLKTPTFRHKAYAGYKATRKGMPEELASQMEPLKKLLTLLGYSLVT
                     CEGFEADDILGTLAKACEENGNECVIATGDRDSLQLVSDKTSVHLCSNKQDILYTPEK
                     ILEAYGVTPKELIEIKAIQGDTSDNIPGVAGIGPKGAGDLIQRYHTVEYIYDHLDELE
                     IKDGVRKKLTASKENAILSRMLGEINCNAPVDTNVENYVVDMKDADECAGFMAKLELF
                     SLIEKYGIKADKNAKPEKVEKNSLEVVSDFDENELLKNVKSEKKLYLNFETENKELKS
                     FAVLFDGKVYISTDEELFVKFIADSELEKYTRNIKTLYAYADEKNIDVENIVFDIELA
                     AYLIEPSSKDYSDKNLCASYEIALPVCTDEQNEKYEAFACYAELCEKLGDKIKAHGQE
                     KLLSEIEIPLAKVLARMENIGVCVDRQGIESYGEMLSAQIKELETAIYESAGCEFNIN
                     SPKQLGVVLFENLGLPCKKKTKSGYSTNAEVLESLRYEHPVVEMVLRYRTLSKLNSTY
                     CEGLLKVIADDGRIHSNFNQTETRTGRISSTEPNLQNIPVRTELGREMRKFFCAREGW
                     ALVDADYSQIELRVLAHISGDKNMIKAFKDNEDIHAITASQVFNMPLDMVTPIMRSRA
                     KAVNFGIVYGIGAFSLAKDIGVSNKEAKHYIESYLAHYSGVDSYMKNVVEKANADGYV
                     ETMFGRRRNLPELTSSKHMIRAFGERVARNMPIQGTAADIIKIAMIKVDERMKAENLR
                     ARLVLQVHDELIVEAPQDESMRVALIVQEEMENAVKMSVPLTADAAIGRTWYDAKG"
     gene            complement(7778..8956)
                     /gene="alr"
                     /locus_tag="PLS64_04165"
     CDS             complement(7778..8956)
                     /gene="alr"
                     /locus_tag="PLS64_04165"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRL43060.1"
                     /translation="MNMVKRTWAEISLNAIEHNYNVIRNKVADDTKVCCVIKADGYGH
                     GAVELSQIYEKLGADFFAVSNIDEGIEIRKSGSKLPIVILGYTPVSEAENLAEYDISQ
                     AVFSLEYAKELSEKCVEEDCICKMHIKVDSGMSRIGFMCQEFPRDEYSIEEICEACCL
                     PNLEVEGLFTHFCVSDEDAEGREFTNKQYENFIHVRDSLKKRGVDISVVHCSNSGAIE
                     DYPETCCDMVRAGIILYGLAPSSKLADRLDLVPAMTLKTVVAFVKEVQKGATISYGRT
                     FTADRKMKIATVPIGYADGFIRQNAKDGYMTVNGKKAKIVGRICMDQTMLDVTDIEDV
                     KTGDEVVVFGTGENGEPTADSLAENTGTINYETVCLVGKRVPRIYIKDGKIENVMYKL
                     "
     gene            9228..10793
                     /gene="putP"
                     /locus_tag="PLS64_04170"
     CDS             9228..10793
                     /gene="putP"
                     /locus_tag="PLS64_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HRL43061.1"
                     /translation="MTTNNIIVLCAFIAYMIIKIVIGFVCSKGNKNNEDYFLGGRNLG
                     SWTAAMSAQASDMSGWLLMGLPGAVYLAGTGEAWIAVGLLIGTILNWYIVSARLRKYT
                     IVAGNSLTIPSFFKNRYRDHKNIIKIVSASIIAVFFTVYTASAFSSGAKLFATLFSDS
                     ASGDENYNRVYVIGLIVAAVVILVYTFMGGFKAVCTTDLIQGLMMIVAILTVPVLAYA
                     ILTFDTSFSSALAAKGVEQPAQFLNFFVNGDGTPVSAVSIISNLAWGLGYFGMPHILV
                     RFMAVKSNEEIKKSRKIAVVWVIISLTASCLIGLIARGYLTAQLDDATSESVFIRTIQ
                     QLFSGNGVLIFIGGIFLCGILAAIMSTADSQLLVTASAVSEDLYKGAVKKNASEKSSL
                     LVGKIAVAVVAVIAFFIALNPKSSIMGLVSDAWAGFGSAFGPVVLLALFWKRSTLSGA
                     ISGMATGALTVIIWDYIPLVNGQTLYAATNLYSLVVGFGLALIVNVIVSLLSKKPSKE
                     IMDEFDSIKNIEV"
     gene            10970..11548
                     /locus_tag="PLS64_04175"
     CDS             10970..11548
                     /locus_tag="PLS64_04175"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRL43062.1"
                     /translation="MKLLEDRILKDGHIGADNVLKVDSFLNHQIDVSFVCELGKEFYR
                     LFKDENITKILTIEASGIGIACLAAQYFNVPVVFAKKTKTINIYSDTYNATVHSYTHK
                     KDYDIVVSKEFLSKEDNVLIIDDFLAKGSALTALLMLIEKAGAKTAGAGIVIEKAYQG
                     GGNLVRDMGIRVESLAKIKSISKKDGIVFCKQ"
BASE COUNT         3094 a         2615 c         2307 g         3598 t
ORIGIN      
        1 aggctctact gttgtaggct ctgctactgt tgtaggctct actgttgtag gctctactgt
       61 tgtaggctct tctgaaccgt cgggaactgt tacagtttta gcaactgcga catacttatc
      121 aaatgcatcc tgatttaata cagatttgtc taagagtact acttcatcac tagggatttc
      181 caactctgat acagggtctt gatttgaaat agtaagatac tctacatcaa ggtcaacagt
      241 agttgctaca gcctcgggat tgatgacatc gaattcaaca gaagaaaata ctatttcttt
      301 ttctgatgaa aaatagagac ttgtgtcact tcctataccc ataagtaaac caggagctac
      361 atttttgttg aattcgaaat ttggatattc gaatgaagta ctctttcttg tattatctga
      421 gaccttgaga acagcaggat catagttcat tttatactgg aagttcaaca taccatctct
      481 gtccttggtc tggaacatgt atgtaaccct tacagtcttc tgatctgcgc tgagagtaac
      541 ctgcttctca gaaaagaggt ttgaagtagc tgtaactgtg agaccctttt ttgcaggagc
      601 tgcagttgta ggctctgcta ctgttgtagg ctctgctact gttgtgggct ctgctactgt
      661 tgtaggctct gctactgttg taggctctgc accagtaacg atgtcaacct taccctttgc
      721 accctgcttt gtagcatagt taaagtttga aaggtcaagt gtaagaacga tgtcataacc
      781 gtcttcgtaa ccagctgcat ctgtatcaat gatcatgttc tgaccgttgt atgtaaggtc
      841 aaaccactca ttgagaggag ctgtgccctg ctcaggaagg ccccagttat ctgcccatga
      901 accgttggca gcaaacttga actgatagtt ctcataagcg tcaaggctct caaacttaat
      961 ctggtaaacc tttgaaccct cagatgtttc tgtcatgtag ttagcttcag catctacctt
     1021 ccagtcctta ccgttgagcc atgattcttc accgttacca acgactgtaa tgtggtcaac
     1081 aacaagttct ttcggaatta caacaccttc acctgttgca gtgatttcat ttgttgtagg
     1141 gttgtaagtt actgttacat cacattcctt tgttacattg aatgtgaagt tgtcattgcc
     1201 ggtatcgcct acaccaatcc actctgtatc gtttttaacg atcttgaact ggtagccgtt
     1261 gccgactgct acgttggtaa atactttttc gtagttacca tcgccgtttt ctgtcattac
     1321 attatctccg caagttgctt cagtctcagc ccacttgtaa cctgtgagtt cttcacaacc
     1381 ggatacgaaa tatgaatcgc cggcagcaga aacgctcact acagttgcaa cgagtgatgt
     1441 cacaacaagc atcatagaca gtaaaaggct aagtgctttt ttgaacattt tcttttcctc
     1501 cttatgtgat aatgcttcta taagcagttt aattatacaa caatcaacca ccaaaagtca
     1561 atgctaaaaa ctcttgccgg ctgattattt gacaaaatat tgtttttttg taggaatgca
     1621 caaataaaat atcatttatt tgttatttgt aaccattcac attgtgagat tgccagttcc
     1681 aactgtcacg gcacatatcc tcaagacttt tctcggcaat ccatccaagt attttcttgg
     1741 cttttgttgc gtcggcaaag cattctgcaa tgtcgcctgc tcggcgtggc tttataacat
     1801 aaggaatttc aatcgaattt gccttttcaa aagcctttac aatatcaaga acgctgtagc
     1861 ccacacctgt tccgagatta aaaatctcgg ttcccttgtg ctctgcggaa tattcaatag
     1921 cctttacatg accttttgca aggtcaacaa catggatata gtcacgcaca cccgtgccgt
     1981 catgcgtagg gtaatcgttg ccgaaaacac cgagacactt gagtctgccg gcggcaacct
     2041 gtgttatgta cggcataagg ttattcggga ttccgttagg atcttcaccg attctgccgc
     2101 tttcgtgagc gccgattggg ttaaagtaac gaagaagaac aacagacata tcgggatttg
     2161 cgtttgcggt gtctgtaaga atttgttcca tcataaactt tgtccagcca tacggatttg
     2221 taggagaacc ctttggcatt gtttcaacaa gcggaacagt ttccggagta ccgtaaaccg
     2281 tggcacttga gctgaaaata aagttattca caccgtgttt tttcatcacc tcaagcaatg
     2341 tgagcgttga atcaatgttg ttacggtaat attcaacagg cttctcgcag ctttcaccta
     2401 ctgctttaag acctgcaaag tgaattaccg catcaatctt attctccgaa aaaatctttt
     2461 caacggcatc tctgtcacat acatcggcct catagagcgg aatttttctg cctgtcagct
     2521 cttcaactct ttcaaccgct acgggacagc tgtttgaaaa attatctgca attacaacat
     2581 cgtgacctgc gttgatgagt tcaacacaag tgtgcgagcc gatatatccg gcgccgcctg
     2641 caagcaaaac attcatttta aaattccttt cttaacaatc agcatttttc aggctcggga
     2701 tagtcaacac caaaggtttc ggctgtaacc tttgccataa tctgaggatc aagaggcttg
     2761 tcggaatagt ctgtgtcaca ctctgcgatt tcattgacaa catccatacc ctcaatcacc
     2821 ttgccgaatg cggcatactg accgtcaagg tgcggagcgt ttttgtgcat aatgaagaac
     2881 tgcgagcctg ctgagtcggg catcattgaa cgagccattg agagaacacc ctctgtatgc
     2941 ttgagattgt tttcaaagcc gttcacggca aattcaccct tgattgaata tccgggaccg
     3001 cccatacccg tgccttcggg gcaaccgccc tgaatcatga aaccgtagat tacacggtga
     3061 aatgtaaggc cgtcatagta acccttgttt acaaggctga gaaaattatt tacagtattc
     3121 ggagcaattt cgggataaag ctccgctttg attgtttttc cgttttccat ttcaaaagta
     3181 acaataggat tgctcatttt aaatcgtcct ttcggtttat taacactatt ttagtatatt
     3241 cttgataaaa tagcaagtgt tttagtgatt atttgcgaat aaatcagcct ttttcaatgc
     3301 tcattgtagc aaaagcattg aggtctgttc ctgcaatatt tccgctgaga aactcgtaat
     3361 atccctgtga accgaccatg gcggcattgt caccgcaaag agatttttcg ggcatataca
     3421 tttcccaatc acgcttttta cactcttctg caagggttct tctgagaagt gaatttgccg
     3481 aaacaccgcc tgcgattaca agctttttct gtcccaagtc ttcagccgcc ttgataaagt
     3541 ttgtaacaag gcaatcgaca acagcatagc ggaatgatgc acagacatcg gctttgttaa
     3601 gctcaacacc cttttgtgcc gaattgtgaa tgaggttgat tacggctgtt tttagtcccg
     3661 aaaagctgaa atcatacggt gcatcgtgaa ctgcgggacg agggagcttg aatgcaaacg
     3721 gattgccgtt ttccgcaact ctgtccattt caattccgcc cggatacggc attcccattg
     3781 tccttgcggc tttgtcaaaa gcctcgcctg cggcatcatc acgggttttg ccgataattt
     3841 tcatctttgt gtagtcctca accataacaa tatggctgtg accgcctgac acaacaaggc
     3901 agagaaacgg cggttttaat tctttatttg aaatataatt tgacgcaatg tgtgaacgaa
     3961 gatggtgaac aggaacaagc ggaattcctg ttgcaagtga aagtcctttt gcaaaattca
     4021 ctcccacaag caacgctccg ataagtccgg gggcatatgt aacgccgatt gcgtcaatgt
     4081 cggaaagcct gcagtctgca tcttcaagag cctgtcttac aacaggaaca attgcctctg
     4141 cgtgacggcg tgatgcgatt tcgggaacaa caccgccgta aagcttgtgt tcctcaacct
     4201 gagaagcaac caccgaggag attatgtttc tgccgtcctc aacaaccgct gccgcagttt
     4261 cgtcacagga cgattctatt gcgagtatct tcataatttt cctttcacta cgaaacattt
     4321 atgatgaagt tccgtaatat cgttaatata atgttttatt acatatatat gtcaaaaaca
     4381 gcgtgtcatt aaaacagcgt tttcggttgg agaggagtag taatttttcc tctctcccac
     4441 cttgataaat ccgaattttt catagagaga acgggcgttc ccgttacttt cacgaacctc
     4501 aagagtgaga aagcaaaggt tatttttctt ggcataggtc acaagcgttt caacaagcgc
     4561 cgaacctatt ccccgatttc tgtgtgtacc gtcaacggca acattgaaaa tgtaaccctc
     4621 atcaacaaca acgctcattc ctatatatcc gacaacggca ttatcttccg ttgcggtaaa
     4681 aaacagcgag gtttcgttgt tcagttcttc ttcaaggctg ttaaacgacc acggatgcga
     4741 aaaacacctc tgttcaagct gtgcaaccgc ctcaagctga ttttcggtca ttctgtcaat
     4801 tttcatacgc ttttaatatt tcctttacag aattaacaat ttcatcacgg caatgtctgt
     4861 acacctcaat gtcgccgccg tagggatcgc ttataccgag cacggcgatt ttttcgggct
     4921 gtacaagaaa aaactgcatc aacatttttg cgtgactttg cgacatacag taaaattttt
     4981 cgtatttttc aatgccggca tcagctacgg ctgttgaagc tttgcccgaa aggtcaattc
     5041 cgatttcctt acaggcaatc acggaatttt ccgacacggg aagtcccgtt gaggtcgcaa
     5101 tgccaaagct ttccgccctg acgcaaacac cgtttttctc tgccatatca ttaaaaattg
     5161 cctctgccat gggactgcga caggtgttgc ccgtgcatac aaaggcaatc gttctgttat
     5221 ccttttgcat cataccaagt ccttccgatt gccgcatcgg ctgtgagcgg aacgctcatt
     5281 ttaactgcgt tttccatttc ttcctgaacg atgagtgcaa ctctcatgct ctcatcctgc
     5341 ggagcttcga caataagttc gtcgtgaacc tgcagcacaa gccttgcacg gagattttcg
     5401 gctttcattc gctcgtcaac cttaatcatt gcaattttga taatgtctgc ggctgtgccc
     5461 tgaataggca tatttcttgc aactctctcg ccgaatgcac ggatcatatg cttgctcgag
     5521 gtgagttcgg gaagatttct tcttctgccg aacatcgttt caacatagcc gtcagcgttc
     5581 gccttttcaa caacattttt catatagctg tcaacaccgc tgtaatgggc aagatagctt
     5641 tcaatgtagt gctttgcctc tttatttgaa acaccgatat cctttgcaag tgaaaatgcg
     5701 ccgattccgt aaacaatacc gaagttaacc gcctttgcac ggcttctcat aatcggagtt
     5761 accatatcaa gcggcatatt gaacacctgt gacgctgtga ttgcgtggat atcttcgtta
     5821 tccttgaacg ctttaatcat atttttgtca cccgaaatat gggcaagcac acggagttca
     5881 atctgcgaat agtcggcgtc aacaagcgcc cagccctctc ttgcacagaa aaactttctc
     5941 atctctctgc caagctcggt tcttacgggg atattctgga gattcggctc tgttgagctg
     6001 attctgcctg ttcttgtttc ggtctgattg aaatttgaat ggattctgcc gtcatctgca
     6061 atgaccttta aaagtccctc gcagtaggtt gaattcagct ttgagagcgt tctgtatctc
     6121 agcaccattt caacaacggg atgttcatac ctcaggcttt caagcacctc tgcatttgtg
     6181 ctgtaaccgc tcttcgtttt ctttttgcaa gggagtccga ggttttcaaa taaaactacg
     6241 ccaagctgtt tgggagaatt gatgttaaac tcacagcccg cactttcgta aattgcggtt
     6301 tcaagctcct taatctgtgc agaaagcatt tcgccgtagc tttcaatgcc ctgtcggtca
     6361 acacagacac cgatattttc cattcttgca agcacctttg caagcggaat ttcaatttca
     6421 ctcaacaact tttcctgacc gtgagccttg attttatctc cgagtttttc gcacaattct
     6481 gcgtaacagg caaatgcctc gtatttttca ttctgctcat cagtgcagac aggcagagcg
     6541 atttcgtaag aggcacaaag atttttgtcc gaataatcct ttgacgacgg ttcaatcaga
     6601 tatgccgcaa gctcaatatc aaacacaata ttttcaacat caatattttt ctcatcggca
     6661 taagcgtaga gcgttttgat gtttcttgtg tacttttcaa gttcactgtc ggcaataaac
     6721 ttaacaaaaa gttcttcgtc cgtgcttata tacaccttgc cgtcaaaaag tacggcaaag
     6781 gattttaatt ctttattctc tgtttcaaag ttgagataga gctttttctc tgacttaaca
     6841 ttttttaaaa gttcattttc gtcaaaatca gacacaacct caagcgaatt tttctcaacc
     6901 ttttcgggct ttgcattttt gtcagccttg atgccgtatt tttctattaa agaaaacagt
     6961 tcaagctttg ccataaatcc ggcacattca tcggcatctt tcatatctac aacataattt
     7021 tcgacatttg tgtcaacagg agcgttgcag tttatttcac cgagcatacg gctgagaatt
     7081 gcattttcct ttgacgcagt cagcttttta cgcacaccgt ccttgatttc aagttcatca
     7141 agatggtcat agatatactc aaccgtgtga tatctctgaa tgaggtcgcc tgcacccttt
     7201 ggacctattc ccgcaacacc cggaatatta tccgaagtat cgccctgaat cgccttgatt
     7261 tctataagct cctttggagt tacaccgtaa gcttcaagaa tcttttcggg ggtataaagt
     7321 atatcctgtt tgtttgagca aagatgaacg cttgttttgt ccgacacaag ctgaaggctg
     7381 tcacgatcac ctgttgcaat aacgcactcg tttccgtttt cttcacaagc cttggcgagc
     7441 gtaccgagaa tatcgtcagc ctcaaagccc tcacaggtaa caagactgta gcctaaaagg
     7501 gtaagaagct ttttcagcgg ttccatttgt gacgcaagct cctccggcat accttttcgt
     7561 gttgccttgt agcctgcata agccttgtgt ctgaaggtcg gtgttttcag gtcaaaggca
     7621 atcgccacgg cgtcgggatt ttcttcattt ttaattttgt acagcattgt aagaaatccg
     7681 taaattgcgt ttgtaaactg accgtccttt gtagtaagcg gtcttattcc gtaaaacgca
     7741 cggtttacaa tgctgtttcc gtcaacagca agtaatctca taatttatac atcacattct
     7801 caatttttcc gtctttaatg taaattctcg gaacacgctt tccgacaaga cagacggttt
     7861 cgtagtttat agttcctgta ttttcggcaa gtgaatctgc agtaggctca ccgttttcgc
     7921 ctgtaccgaa tacgacaacc tcgtcacctg tcttgacatc ttcaatatcg gtaacatcaa
     7981 gcattgtctg atccatacat attctgccca caattttcgc tttctttccg tttaccgtca
     8041 tatagccgtc ttttgcattc tgtctgatga atccgtcagc gtatccgata ggaacggttg
     8101 caattttcat ctttctgtcg gctgtaaaag ttctgccgta ggaaattgtt gcaccctttt
     8161 gaacctcttt tacaaaagcg acaactgttt taagagtcat tgcaggaaca aggtcaagtc
     8221 tgtcggcaag ctttgatgac ggagcaagtc cgtagaggat aatacctgct cttaccatat
     8281 cgcaacaggt ttcggggtaa tcttcaattg cgccgctgtt tgagcaatga acaacgctga
     8341 tatcaacgcc tcgcttttta aggctgtcac gcacatgaat aaagttttca tactgcttgt
     8401 ttgtgaactc tctgccctcg gcatcttcat cagacacaca aaaatgagta aaaagcccct
     8461 caacctcaag attcggcagg caacaagcct cgcaaatttc ttcaatagaa tattcatccc
     8521 gtggaaattc ctgacacata aagccgattc ttgacattcc cgagtcaacc ttgatgtgca
     8581 ttttgcagat acaatcctct tcaacacatt tttcggaaag ctcctttgca tactcaagtg
     8641 aaaaaacagc ctgagaaata tcatattcag caagattttc agcctctgaa acgggagtgt
     8701 agccaagaat cacaataggg agttttgaac cgctttttct gatttctata ccctcgtcaa
     8761 tatttgaaac agcaaaaaaa tctgctccga gtttttcata aatctgtgaa agctcaactg
     8821 caccgtggcc gtagccgtca gcctttataa cacaacagac ctttgtatcg tctgcaacct
     8881 tatttcttat tacattatag ttatgctcaa ttgcattaag tgaaatttca gcccaagttc
     8941 tttttaccat attcatttct atctctccac atacctttta cagtcaattt gcaatcaatg
     9001 taattataat acttttgatt cttcaaatca acacaaaatc cccctttaaa tgcatataaa
     9061 agttatttta ttcgtaatta tgtgcaaaat gttactcctg aatttttgca aatgcacaaa
     9121 tcatctgcat aaaagcccta aatactgcaa ttcacttgat ttatttattc atttttggta
     9181 gaattaaaag gtatttgatt tataaattta atttcaggag gggcataatg acaacaaaca
     9241 acataatagt tctttgtgcg tttatagctt atatgattat taagatcgta ataggctttg
     9301 tttgctcaaa gggcaacaag aacaatgagg actactttct cggtggcaga aacctcggca
     9361 gttggacagc cgcaatgtcg gcacaggcat ctgatatgag cggttggctt ttgatgggac
     9421 ttccgggcgc tgtatacctt gccggcacag gcgaggcatg gattgccgta ggacttctta
     9481 tcggcacaat tctcaactgg tacattgttt cggcaagact tcgtaaatac acaattgtgg
     9541 caggcaactc gctcactatt ccgagctttt tcaaaaatcg ctaccgtgac cataaaaaca
     9601 taatcaagat tgtttcggcg tcaatcatcg cagttttctt cacggtttac acagcgtcgg
     9661 cattcagttc gggtgcaaag ctttttgcaa cactcttctc ggacagcgca agcggtgacg
     9721 aaaactacaa tcgggtttat gttatcggtc ttatcgttgc cgcagtagta attcttgtat
     9781 atacattcat gggcggtttc aaggcagttt gtacaaccga cctcattcag ggacttatga
     9841 tgattgttgc aatccttacc gttccggtgc ttgcatatgc aattctcaca tttgacacaa
     9901 gcttcagttc tgcacttgcc gcaaaaggtg ttgaacagcc tgctcagttc cttaacttct
     9961 ttgtaaacgg tgacggaact cctgtttcgg ctgtttcgat tatttcaaac cttgcgtggg
    10021 gactcggtta cttcggtatg ccgcatattc tcgtcagatt catggctgta aagagcaatg
    10081 aagaaatcaa gaagtcaaga aaaattgctg ttgtttgggt aattatttca cttactgcgt
    10141 catgccttat cggtcttatc gcaagaggct accttacagc acagcttgac gatgcaacaa
    10201 gcgaatctgt attcatcaga acaatccagc agcttttctc aggcaacggt gtgcttatct
    10261 ttatcggcgg tattttcctt tgcggaatcc ttgccgcaat tatgtcaaca gcagacagtc
    10321 agcttcttgt cactgcatcg gctgtttcgg aggatcttta caaaggtgct gttaaaaaga
    10381 atgcaagcga aaagagttcg cttttggtcg gcaaaattgc agttgcagtt gttgctgtaa
    10441 tcgcattttt cattgcactt aatcctaaat caagcattat gggacttgtt tcggatgcat
    10501 gggcaggatt cggttcggca ttcggacctg ttgttctgtt ggcacttttc tggaaacgct
    10561 caacactttc gggtgcaatc agcggtatgg caacaggtgc tctgacagtt atcatatggg
    10621 actatattcc gcttgtaaac ggtcagacgc tttatgcggc aacaaatctt tattcacttg
    10681 ttgtaggctt cggacttgcg cttattgtga atgttattgt ttctcttctt tcaaaaaagc
    10741 cgtcaaaaga aatcatggac gaatttgatt caattaaaaa tattgaagta taaccgcagg
    10801 ggcagtcgct ttatcacgac tgcctttttt gtgtctgaat ttaatttttc ccgaaaataa
    10861 gaaaaataag gcatggaata tcacaaaaat caattgaaat gtgcaaatat ttgtagtata
    10921 atctatcgta ttgtttgtta ctattttatt tttcagaaaa gagattgata tgaaactttt
    10981 agaagacaga attcttaaag acggacatat tggtgccgac aatgtgttaa aggttgacag
    11041 ctttctcaat catcagattg atgtcagttt tgtatgtgag cttggcaagg aattttacag
    11101 acttttcaag gatgaaaaca taacaaaaat tctgacaatc gaagcgtcgg gaatcggcat
    11161 tgcctgtctt gcggctcagt attttaatgt tccggttgtt tttgcaaaaa agacaaagac
    11221 aatcaatatt tattccgata catacaacgc aaccgtccac tcatacactc ataaaaagga
    11281 ctatgacata gttgtttcta aggaattttt gagcaaagaa gacaatgtgc ttatcattga
    11341 cgattttctt gcaaagggca gcgccttaac ggcacttctt atgctgattg aaaaagcagg
    11401 cgcaaaaaca gcaggtgcgg gaattgttat cgaaaaagct tatcagggcg gcggcaacct
    11461 cgttcgtgat atgggcataa gagttgaatc tttggcaaaa atcaagtcaa tcagtaaaaa
    11521 agacggaatc gtgttctgca agcagtagta atatactgct tgcttatact tgacttttgc
    11581 tgatatttat agtattatct aacggtaggt cttg
//
LOCUS       DAOMVE010000049        11590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1314_length_11590_cov_3.194452, whole genome shotgun sequence.
ACCESSION   DAOMVE010000049 DAOMVE010000000
VERSION     DAOMVE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1314_length_11590_cov_3.194452"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="PLS64_04180"
     CDS             <1..1470
                     /locus_tag="PLS64_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRL43063.1"
                     /translation="SLNLGIPVVKTCATDKKGLDELKKAIYNVCSGKTKCFRVERNFE
                     GVDILSEKNYKQNIEKLSQIGNKISSDCVKYEKSELNACTRKLDKILTSKTTGIPIML
                     AMLGILFWITVVGANYPSEWLSNLFGFIKEKLYILFDFLHAPDVVTGLLIDGVYTTLS
                     WVVSVMLPPMAIFFPLFSLVEDSGYLPRIAFNLDRYFSKCGAHGKQSLAMAMGLGCNA
                     CGVTGCRIIESPKERLIAILTNNFMPCNGRFPTIIALLLMFFAGTAFTLSSSLEVAAL
                     LIGIIVFCVFITLVISKVLSVTILKGEQSGFVLELPPYRKPQILKTIVRSLLDRTLFV
                     LGRAVAVAAPAGAIIWILANVHISDVSLLKYCTDFLDPFGRFIGVDGVIIMAFILGFP
                     ANETVIPIIIMSYMASGTLVDYSSYDQLFQLLSMNGWTITTAVCTIILCILHYPCSTT
                     CLTIKKETGSLKWTLVSMALPTAMGIVLCMITAGVMRLF"
     gene            complement(1462..2271)
                     /locus_tag="PLS64_04185"
     CDS             complement(1462..2271)
                     /locus_tag="PLS64_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL43064.1"
                     /translation="MNEKIIMLGTGSAMVTDCYNTCFLLKSENDCLLVDAGGGNGILS
                     AIKKSGIAWNSIKTMFITHSHTDHILGAVWVIRQISALMNSGKYDGNFNIYCHDECVD
                     SITAICNHTLAPKFLKNINTKIFINEVKDGEKQNVGSIRLTFFDIFSDKTKQFGFKAV
                     FPSGKVLACLGDEPFNEKCRNFVQNCDYLMCEAFCLYSQKDIFKPYEKFHSTALDAGK
                     LAESLFAKNLILYHTEDETLQTRKRTYTSEARTVFNGNIFVPDDFEVIDLK"
     gene            complement(2337..3710)
                     /gene="scfB"
                     /locus_tag="PLS64_04190"
     CDS             complement(2337..3710)
                     /gene="scfB"
                     /locus_tag="PLS64_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HRL43065.1"
                     /translation="MIHQYKLNGYNIVLDVYSGSVHAVDDLAYDVIAMYKNATKEQII
                     DEMLSKYADNPEITKEEISDCIDDVKELEEQGKLFTDDKYENLAFDFKKRNTVIKALC
                     LHIAHTCNLNCEYCFASQGKYHGERALMSFEVGKRAIDFLIENSGSRVNLEVDFFGGE
                     PLMNFDVVKQIVAYARSIEKEHNKNFRFTLTTNGMLVDDDVIEFANKECHNVVLSLDG
                     RKEVHDHLRKTVNGKGSYDIIVPKFQEFVKKRGNKGYYVRGTYTHNNTDFTNDIFHMA
                     DLGFTELSMEPVVCAPDDPYALTYDDLPILFEQYEILAKEMLKREKEGRPLTFYHYMI
                     DLTGGPCIYKRISGCGSGTEYMAVTPWGDLYPCHQFVGDEKYKLGDIFNGVSNTKIQD
                     EFKLCNAYARPDCKDCWARLYCSGGCAANAYHATGSITGIYEYGCELFRKRMECAIMM
                     KVAEAEE"
     gene            complement(3792..3944)
                     /gene="scfA"
                     /locus_tag="PLS64_04195"
     CDS             complement(3792..3944)
                     /gene="scfA"
                     /locus_tag="PLS64_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HRL43066.1"
                     /translation="MKRIKTIETRDLKKSVKTGGCGECQTSCQSACKTSCGVANQQCE
                     KCMSEK"
     gene            complement(4006..5409)
                     /locus_tag="PLS64_04200"
     CDS             complement(4006..5409)
                     /locus_tag="PLS64_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL43067.1"
                     /translation="MLYDKLKNYSKSGIYPFHMPGHKRTDITEEGIIPYNIDITEIHD
                     FDNLHSPNGVIDEIQKKAAKLYNAKNAFILINGSTGGILSAIRSMTNQGDKILMARNC
                     HKSVYNAAELFNLNADYIFPDTDSRYNILTSVSPCDIEDKLTKHNDEIRLVIITSPTY
                     EGVVSDIKSISEICHRHGAKLLVDEAHGAHFPFSDNFPDEALNCGADAAVLSLHKTLP
                     SLTQTALLITNDSELSEILAENLAVFETSSPSYILMSSIEKCLDFCENSKDKFNEYCC
                     NLKTVREKLKNLKYLKIYDKSDTDFDYDIGKIVISTANANISGTKLAEILRNNYQIET
                     EMAYSDYVIAMTSVCDTEKAFDMLSDALISIDSKLSKGADTERVPLKNLYTGKNFNAC
                     ELYKFNKETIPFQNSEFRTSAEYIWAYPPGIPLIVPGEIISKELINYIEYLSACKVEI
                     MSTKGGMTDNISVVKKD"
     gene            complement(5433..7019)
                     /locus_tag="PLS64_04205"
     CDS             complement(5433..7019)
                     /locus_tag="PLS64_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HRL43068.1"
                     /translation="MSSFKEEISKRRTFAIISHPDAGKTTLTEKLLLYGGAINLAGSV
                     KGKRTAKHAVSDWMEIEKQRGISVTSSVLQFKYNGYCINILDTPGHEDFSEDTYRTLM
                     AADSAVMVIDGSKGVEKQTIKLFKVCVMRNIPIITFINKMDRDAKNSFDLLEDIENVL
                     GIHTYPVNWPIGSGKEFKGVYDRNSKKILAFTANNGQKEVEKKELTLDDPALEDTIGY
                     YHKQDLFDEIELLDGAGDEFDLEAVRNGQLTPVFFGSALTNFGVEPFLEQFLQLTTPP
                     LPRETVDEKVEPMSDFFSAFVFKIQANMNKAHRDRVAFMRICSGKFEKNMEVFHVQGN
                     KKMRLSQPQQIMAQEREIVDEAYAGDIIGVFDPGIFSIGDTICSPGHKVQFRGIPTFA
                     PEHFALVRQKDTMKRKQFIKGTSQIAQEGAIQIFQELDAGMEEVIVGVVGVLQFDVLK
                     YRLQNEYNCDIIMETLPYEFIRWIVNDDLDPKTLDLASDTKKIQDLKGNHLLLFTSYW
                     SINWALEHNKGLELAEFGTN"
     gene            7269..8369
                     /gene="hemW"
                     /locus_tag="PLS64_04210"
     CDS             7269..8369
                     /gene="hemW"
                     /locus_tag="PLS64_04210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRL43069.1"
                     /translation="MSNSIGLYIHIPFCKHKCPYCDFFSGNADENAFDNYVIELKDKI
                     KYWSEKAKRDVATVYFGGGTPSVLGADRLCDILDFIKFNFNIQNNAEITVEVNPDSAK
                     TIDFKKMYACGFNRISMGMQTAVEDELRLLGRIHSIDDAKTSVERAKSAGFNNISLDL
                     MMGIPNQTIESLEKSISFCADCKVTHISSYILKIEENTPFYKVQNKLKLADDDMQAEM
                     YLRAVEMLDSLGYKQYEISNFAKHGYESRHNTNYWRCGEYIGIGPSAHSFFEGKRFFY
                     SRSMDDFNNNKLSFEGTGGDEEEFIMLSLRLKSGLNYSEFEEKFGYTLPSYIIKKAKE
                     YEKYGYTNVTDKSISFTPKGFLVSNSIISELI"
     gene            complement(8429..9328)
                     /locus_tag="PLS64_04215"
     CDS             complement(8429..9328)
                     /locus_tag="PLS64_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43070.1"
                     /translation="MKFCKYCGAQLEDNAVCNCEKSRAAQAQAQQANAQAQPQYQQPV
                     QPQQPQYQNPVQQQYTQPQNPQTQQSPYAVNNTAPIGENAFVKALKNIPVVFTSFFKD
                     SKTVIKTAKAEKDIILGALFSAIFFIALILGNMFLMLSMSALDFPKTLLVSLVTAVLI
                     GGFYSVILFAYVKKYKTEENTAKAFIDSFITFSIESIPASIGWILAGLCALISVYMFL
                     FFFVIVMLYLVISLVNQVKANVPEAKNSALFTLILTLIVAVAFMIVFFIGAKLTMWSF
                     NTGTFTTSVLTSLIGGGSYPSLY"
     gene            complement(9580..10287)
                     /locus_tag="PLS64_04220"
     CDS             complement(9580..10287)
                     /locus_tag="PLS64_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43071.1"
                     /translation="MDNSSNMAVNTTPTANQINPEHHYGLDKNKACLNNSCPSNTADS
                     GCYIPAIDEIMRHNQGAPSVAHPNMTEKVQKSNDNPSLEQAEIAYEEGGSTSEEQPEN
                     INKYSGSLQNIPFIKNLKKIDTSIPVLPATPATTASSGATVATPIRQNIGMSMSDYQY
                     PFPVTAENLRYHNGFMRTQIGRRITVDFLMGANTIVQKTGYLLGVAQDYILINEIDTN
                     DITTCDYYNIKFIRYYY"
     gene            complement(10302..10745)
                     /locus_tag="PLS64_04225"
     CDS             complement(10302..10745)
                     /locus_tag="PLS64_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HRL43072.1"
                     /translation="MALSTRELLARLLKCEAGGEGENGMRAVASVIVNRTRVPNGEFA
                     RVSKGGDFRAIMEQPNQFTCLKTTVGGQYNPQNVYNIRPDEIHYNIADWALAGNTDSS
                     VGNSIFYFNPFSDTCPTFFPSNNGVIYNRVGDHCFYSPTEAYSKT"
     gene            complement(10939..11256)
                     /locus_tag="PLS64_04230"
     CDS             complement(10939..11256)
                     /locus_tag="PLS64_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43073.1"
                     /translation="MDEEYDDWLVNDDGKNNINDLIKETVALETTGKTGFFYLDKKRT
                     QNIFKKSGYQLPSQLKNSGSNIIIRTIDDSVNKKSIILLKANNLSDGSVIGKIALQKQ
                     VKR"
     gene            complement(11337..11486)
                     /locus_tag="PLS64_04235"
     CDS             complement(11337..11486)
                     /locus_tag="PLS64_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43074.1"
                     /translation="MNLYSDNTTLRVSNSMTSDETLLKMEKLMKDNPTEQELIKRLGK
                     NFPQE"
BASE COUNT         3311 a         2168 c         2273 g         3838 t
ORIGIN      
        1 tcgctaaacc tcggaatacc tgttgtgaaa acctgtgcaa cagacaaaaa aggtctggat
       61 gaattaaaaa aagcgatcta taatgtatgc agcggaaaga caaaatgttt tagggtagaa
      121 agaaattttg aaggcgttga tatactatcc gaaaaaaact acaaacagaa tattgaaaag
      181 ctttcgcaga ttggcaacaa aatttcaagt gactgcgtaa aatatgaaaa atcagagtta
      241 aatgcttgta caagaaagct tgataaaata cttacaagca aaaccacggg aataccgatt
      301 atgcttgcaa tgcttggtat attgttctgg ataacagttg tcggtgcaaa ttatccgagc
      361 gaatggttaa gcaatttgtt tggctttata aaagagaaac tgtatattct ttttgatttt
      421 ttacacgctc ccgatgttgt aacgggactt ttgattgacg gagtttatac aactctgtca
      481 tgggtggtat ctgtaatgct gccgcctatg gctatattct ttccgttgtt ttcgcttgtt
      541 gaggattcgg gatatctgcc gagaattgca tttaatcttg ataggtattt ttcaaagtgc
      601 ggtgctcacg gaaagcaaag tcttgctatg gcaatgggtc ttggctgtaa tgcctgcgga
      661 gttacaggtt gcagaatcat cgagtcgcca aaggaacgac tgattgcaat tcttacaaat
      721 aattttatgc cgtgtaacgg acggtttccc acaataattg cattactgct catgttcttt
      781 gcaggtacgg catttacgct gagcagttcg cttgaggtag cggcattgtt gattggaata
      841 atagttttct gtgtttttat aacacttgtt atatctaagg tactttcagt cacaatattg
      901 aaaggcgaac agtccggatt tgtgcttgaa cttccgccgt acagaaagcc gcagatttta
      961 aaaactattg tccgttcgct gttggacaga actttatttg ttttaggaag ggctgttgcg
     1021 gttgccgctc cggcaggtgc gataatctgg attcttgcaa atgtgcatat aagcgatgtt
     1081 tcacttttaa aatattgtac tgatttttta gatccgttcg gcagatttat cggggttgac
     1141 ggcgttataa taatggcatt tatacttgga tttcctgcga atgaaaccgt tattccgatt
     1201 ataattatgt catatatggc aagcggtact cttgttgatt attcaagcta cgatcagctg
     1261 tttcaacttc tcagtatgaa cggctggacg attacaacgg ctgtatgcac gattattctt
     1321 tgcattcttc attatccgtg ttcaacaacc tgtctgacaa taaaaaaaga aacaggcagc
     1381 cttaaatgga cgcttgtttc tatggctttg cctactgcaa tgggaattgt gctttgtatg
     1441 attacagcag gtgttatgag attattttaa gtcaataact tcaaaatcat cgggaacaaa
     1501 tatatttccg ttaaaaactg ttctcgcctc tgaagtatat gttcttttgc gtgtttgaag
     1561 tgtttcgtcc tcggtgtgat acaggataag attttttgca aaaagactct ctgcaagttt
     1621 tcctgcatca agtgctgtgc tgtggaattt ttcgtaaggc ttaaaaatat ctttttgact
     1681 gtaaagacaa aaagcttcac acataagata atcacagttt tgaacaaaat ttctgcattt
     1741 ttcattgaac ggttcatcac cgagacaggc gagaactttt cctgacggaa aaacagcctt
     1801 gaatccgaat tgctttgttt tgtctgaaaa tatatcaaag aaagtgagtc ggatactgcc
     1861 gacattttgt ttttcaccgt cttttacttc attgatgaaa atttttgtat tgatattttt
     1921 gagaaatttc ggtgcaagtg tgtggttgca aattgccgtg attgaatcaa cacattcatc
     1981 gtggcagtaa atattaaaat taccgtcata tttgcctgaa ttcataagag cgcttatttg
     2041 ccttattacc cacacggcac ctaaaatatg gtcggtgtga gaatgagtaa taaacattgt
     2101 ctttatgctg ttccaagcta ttccggattt cttaattgcg gataaaattc cgtttccgcc
     2161 gcctgcatca acaagaagac agtcgttttc ggatttcaga agaaaacagg tattataaca
     2221 gtcggtaacc atcgcacttc ctgtgccgag cataataatt ttttcgttca tacagcacct
     2281 cgaaaataca ggcagataca agaaaaatgt atctgcctgt aaattttatt gtaattttat
     2341 tcttctgctt ctgcaacctt catcataatg gcacattcca ttctttttct gaaaagctca
     2401 cagccgtatt cgtaaatacc tgtgattgag cctgttgcat gataagcgtt tgcggcacaa
     2461 ccgccgctgc aataaagtct tgcccagcag tctttgcagt caggacgggc gtatgcgtta
     2521 caaagcttaa attcgtcctg aattttcgtg tttgaaacac cgttaaagat atcgccgagc
     2581 ttgtactttt catcgcctac aaactgatga cagggataga ggtcgcccca aggtgttaca
     2641 gccatatatt ctgtacccga accgcaaccc gagattctct tataaatgca cggaccgcct
     2701 gtaaggtcaa tcatataatg atagaaggtg agcggtctgc cctctttttc acgcttgagc
     2761 atttcctttg caagaatttc gtactgttca aaaaggatag gcaaatcgtc atatgtaagt
     2821 gcatatgggt catcgggagc acatacaacc ggctccattg aaagctctgt aaatcccaaa
     2881 tccgccatat ggaaaatatc gtttgtgaag tctgtgttat tatgtgtata agtgcctctt
     2941 acatagtaac ccttgttacc tctttttttg acaaattcct ggaatttcgg aacgataata
     3001 tcataactgc ctttgccgtt tacagttttg cgaagatggt catgcacttc ttttctgccg
     3061 tcaaggctga gaactacatt atgacattct ttgtttgcaa attcaattac atcgtcatca
     3121 acaagcatac cgtttgttgt aagtgtaaaa cggaagtttt tgttgtgttc cttttcaatg
     3181 cttcttgcat aggcaacaat ctgctttaca acatcaaagt tcataagagg ttctccgccg
     3241 aagaagtcaa cctcaaggtt tactctgctg cctgaatttt caatgagaaa gtcaattgct
     3301 ctcttgccca cttcaaagct catcagcgct ctctcgccgt ggtatttgcc ctggcttgca
     3361 aagcagtatt cgcagttaag gttacaggtg tgagcaatgt gcagacaaag tgctttgatt
     3421 actgtgtttc tctttttaaa gtcaaatgca aggttttcgt acttgtcgtc tgtaaagagc
     3481 tttccctgtt cctcaagctc ttttacatca tcaatgcagt cggagatttc ttcttttgta
     3541 atctcaggat tgtctgcata ttttgaaagc atttcgtcga ttatctgttc ttttgttgcg
     3601 tttttgtaca ttgcaataac atcataagca aggtcatcga ctgcgtgtac actgccgctg
     3661 taaacatcaa gaactatgtt gtatccgtta agtttatact gatgaatcat aatatctccg
     3721 ttagacaaaa ggaaagccgt taaccctttg ggaaaacggc gataatcctt aataatcaaa
     3781 ttaaaataga attatttctc gctcatgcac ttttcgcact gctggttagc aacgccgcaa
     3841 gatgtcttac acgctgactg acatgaagtc tggcactcgc cgcaaccgcc tgtcttaacg
     3901 ctcttcttga ggtcacgagt ttcgatagtt ttaattctct tcataataaa tcaaactcct
     3961 tgttagattt tgttagttta atattatcac acagccattg atttgtcaat cttttttaac
     4021 tacgcttatg ttgtccgtca taccgccttt tgttgacata atctcaacct tgcaggctga
     4081 aagatattca atgtaattta taagttcctt gcttataatt tcaccgggta cgatgagggg
     4141 aattccgggt ggatatgccc agatatactc ggcagaagtt ctgaattccg aattttgaaa
     4201 cggaatagtt tctttgttga atttgtacaa ctcgcaggcg ttgaaatttt tgcctgtgta
     4261 gagatttttt aacggaacac gctcggtgtc ggctcctttt gaaagtttgc tgtcaattga
     4321 aatcagagcg tctgaaagca tatcaaaagc tttttcagtg tcacaaacag atgtcatggc
     4381 aattacatag tccgaatatg ccatttcggt ttcaatctga taattatttc gcagtatttc
     4441 ggcaagcttt gttcccgaaa tatttgcgtt cgctgttgaa attacaattt tgccgatatc
     4501 ataatcaaaa tcagtatcgc ttttatcata aattttcaga tattttagat ttttaagttt
     4561 ttctctgacc gttttaagat tgcaacaata ttcattgaat ttatctttgc tgttttcgca
     4621 gaaatcaaga catttttcga ttgaactcat taaaatatat gacggactgc ttgtttcaaa
     4681 tacagccaaa ttttcggcaa gaatttcaga aagctcgctg tcatttgtta tgagcaatgc
     4741 agtttgagtt aatgacggca aagttttgtg cagactcaat actgcggcgt ctgcaccgca
     4801 gttaagtgct tcatcgggaa aattgtccga aaacggaaaa tgcgctccgt gagcttcgtc
     4861 aacaagtaat tttgctccgt gcctgtgaca gatttcggaa attgacttta tatccgaaac
     4921 aactccttca tatgtaggcg aagttatgat tacaagtctg atttcatcat tgtgttttgt
     4981 aagtttatct tctatatcgc aaggtgaaac cgatgtaaga atattgtatc tgctgtctgt
     5041 atcgggaaaa atgtaatcag cattgagatt aaacagttcc gctgcattat atacagattt
     5101 gtggcagtta cgagccatta agatcttgtc gccttgattt gtcattgagc gaattgctga
     5161 aagtattccg cctgttgaac cgttaatcag aatgaaagca ttttttgcat tgtaaagttt
     5221 tgcagccttt ttctgaattt catcaataac tccgtttggt gaatggagat tgtcaaaatc
     5281 atgaatttcg gttatgtcga tattatacgg tattataccc tcttctgtaa tgtccgttct
     5341 tttgtgtccg ggcatatgaa aaggatatat gccgcttttt gaataatttt taagcttgtc
     5401 gtacagcata tatcctccga tattttaaat atttagtttg taccgaactc agcgagttca
     5461 agtcccttgt tgtgttcaag cgcccagttt atgctccagt agcttgtaaa gagcaaaaga
     5521 tgattgcctt taagatcctg aattttctta gtatcggacg caagatcgag agttttcgga
     5581 tcaaggtcat cgtttacaat ccaacggata aattcatatg ggagtgtttc cataatgata
     5641 tcacagttgt actcgttttg aagtctgtat ttaagaacat caaactgaag tacgcctaca
     5701 acgccaacga taacttcctc catacctgcg tcaagctcct ggaaaatctg aattgcacct
     5761 tcctgtgcaa tctgacttgt acccttgatg aactgtttgc gcttcattgt gtctttctga
     5821 cggacaagtg caaagtgttc gggagcaaat gtaggaattc ccctgaactg taccttgtgt
     5881 cccggtgaac agattgtgtc accgattgag aaaatgcccg gatcaaacac accgatgatg
     5941 tcgcccgcat atgcctcgtc aacaatttct ctttcctgag ccataatctg ctgtggctgt
     6001 gaaagacgca tttttttgtt gccctgaaca tggaacactt ccatattctt ttcaaacttg
     6061 ccgctgcaga tgcgcataaa tgcaactctg tcacggtgag ccttattcat gtttgcctga
     6121 attttgaaaa caaatgcaga gaaaaagtct gacataggct caaccttttc atcaactgtt
     6181 tctcttggca gaggtggagt tgtgagctgt aagaactgtt caaggaatgg ttcaacacca
     6241 aagtttgtaa gagccgaacc gaagaataca ggagtaagct gaccgttgcg gactgcttca
     6301 aggtcaaatt catcgcctgc accgtcaaga agttctattt catcaaacaa atcctgctta
     6361 tgatagtagc ctattgtatc ttcaagggca ggatcgtcaa gcgtaagttc tttcttttca
     6421 acttctttct gaccgttatt agcggtgaaa gcaaggattt tcttagaatt tctgtcatac
     6481 acacccttga attccttacc tgaaccgatt ggccagttta cgggataggt gtgaattccg
     6541 agaacatttt caatgtcttc aagaaggtca aaagaatttt ttgcatctct gtccatcttg
     6601 ttgatgaatg taatgatagg aatattacgc attacacaga ccttgaaaag tttgatagtc
     6661 tgtttctcaa caccctttga accgtcaatt accattacgg cagagtctgc cgccataagt
     6721 gtacggtagg tatcttccga gaaatcctca tgtcccggtg tgtcaagaat atttatgcaa
     6781 taaccgttat atttaaactg caaaaccgat gatgtaacag aaataccacg ctgtttttca
     6841 atttccatcc agtcggacac agcatgcttt gcggttcttt tgccttttac cgaacctgca
     6901 aggttaattg ctccgccgta gagcaagagc ttttccgtaa gtgttgtttt acccgcatca
     6961 ggatgcgaaa taatcgcaaa ggttctgcgc ttgctgattt cttctttaaa tgaactcata
     7021 tctttcttcc cttcggaggt taagtatatc cacaattcta tattttacta tttttggatg
     7081 gtatatgtca ataaagaatg aagaaaatgt gagaaaaata attgctacaa aaacttgaca
     7141 aatttgtaaa tatattgtaa tatattggta cagaaggact taatgtgatt tttcctattt
     7201 tttcatatac cttccaaatc gaggcagaaa cttgtgtttc tgcctcaaaa tcatttatga
     7261 ggttttttat gagtaattct atcggtcttt acattcacat accgttttgt aagcacaaat
     7321 gtccgtattg cgattttttc agcggcaatg cagacgaaaa cgctttcgat aattatgtga
     7381 ttgaattaaa agataaaata aaatactggt ctgaaaaagc caaaagagat gttgctaccg
     7441 tatatttcgg cggcggtacg ccgagtgttt tgggtgcaga cagactttgt gatattcttg
     7501 attttatcaa gttcaatttt aatattcaaa ataatgccga gattacagtt gaagtcaatc
     7561 ccgactccgc caaaactatt gattttaaaa aaatgtatgc ctgcggattt aatcgcattt
     7621 caatgggtat gcagactgct gttgaagatg aattaaggct gcttgggaga attcacagca
     7681 tagatgacgc aaagacttct gttgaaaggg ctaaaagtgc gggatttaat aatatttcac
     7741 ttgatttgat gatgggaatt cctaatcaga caattgaaag ccttgaaaaa tcaatcagtt
     7801 tctgtgccga ttgcaaagtt actcatattt catcatacat tttgaaaatc gaggagaata
     7861 ctccgtttta taaggtgcaa aataaactta agcttgccga tgatgatatg caggccgaaa
     7921 tgtatctgag agctgttgaa atgcttgatt ctctcggata caagcaatat gaaatttcta
     7981 attttgcaaa acatggttat gaaagccgac ataataccaa ttactggaga tgcggtgaat
     8041 atatcggcat aggtccgtct gcacactcat tttttgaggg caaaaggttc ttttattcga
     8101 gaagcatgga cgattttaat aacaataagc tgtcttttga aggtacgggc ggagatgagg
     8161 aagaatttat tatgctctct ctcaggctga aaagcggtct taattattct gaatttgagg
     8221 aaaaattcgg atacactctc ccctcttaca ttatcaaaaa agctaaagag tatgaaaaat
     8281 acggctatac aaatgttaca gacaagtcaa tcagctttac accgaaaggt tttcttgttt
     8341 caaattcaat aatcagtgaa ctgatataaa aaacgctccg agaataaatt cggagcgtta
     8401 attttatgtt atgaatttgt atgtcagatt aatacaatga agggtatgaa ccgccgccga
     8461 taagcgatgt aagaacactt gttgtaaatg tacctgtgtt aaatgaccac atagtaagtt
     8521 ttgcgccgat gaagaataca atcataaatg caacggcaac gatgagcgtg agaattaaag
     8581 tgaataatgc agagttcttt gcttcgggaa cattagcttt tacctgattt acaagtgaaa
     8641 taacaaggta aagcataacg attacgaaga agaacaggaa catatatacg ctgattaatg
     8701 cgcaaagacc tgccaaaatc caaccgattg atgccggaat agattcaatt gaaaatgtga
     8761 tgaatgaatc aataaatgct ttggctgtgt tttcttcggt tttgtatttc tttacatatg
     8821 caaagagaat aacagaatag aagccgccga taaggacagc agtaacaaga cttacaagaa
     8881 gtgttttcgg aaaatcaagt gcgctcatag aaagcataag aaacatattg ccaagaatga
     8941 gtgcaataaa gaaaattgcc gagaaaagag cgccgagaat gatgtccttt tcagcctttg
     9001 ctgttttgat aacagttttg gaatccttaa agaatgaagt gaacacaaca ggaatgttct
     9061 ttaaagcctt aacaaaagca ttttccccaa tgggtgctgt gttatttacg gcataaggcg
     9121 actgctgtgt ctgagggttt tgcggctgtg tatactgttg ctgaacaggg ttctgatact
     9181 gtggctgttg tggttgaaca ggctgctgat actgaggctg tgcctgtgca tttgcctgct
     9241 gagcctgtgc ttgagcggct cttgactttt cgcagttgca aacagcattg tcttcaagct
     9301 gtgctccgca atatttacag aatttcatgg tgattcctcc aagataaaat aatatgtaac
     9361 aggtcttata gctacctgtt gtttgtttat tgagaagtga taacatcaac gctcacatta
     9421 tatcacaaat tacagcaata attcaatatt tcttacaatt ttttaatttt ttacactaca
     9481 gaaaattata tgttaaaata atgaaatgat gtacagttcg caaataatta caataaaaca
     9541 gcggcaaaaa ctataaactt tagtttttgc cgctgtggtt tagtaataat accttataaa
     9601 ttttatattg tagtaatcac aggttgtgat gtcgttggtg tcaatctcat ttataaggat
     9661 ataatcctga gcaacaccta aaagatagcc tgttttctga acaattgtgt ttgctcccat
     9721 gagaaaatca acggtgattc gtcttccgat ttgtgttcgc ataaaaccgt tgtgatatct
     9781 cagattttcg gctgttacag gaaacggata ttgataatca ctcatcgaca ttccaatatt
     9841 ctgccttatc ggcgttgcaa cggtggctcc ggagctcgcc gttgtagcag gggttgcagg
     9901 caaaacaggt atagatgtat ctattttttt taagttctta atgaacggta tattttgaag
     9961 acttccgctg tatttattta tattttcagg ttgttcttct gatgtacttc cgccctcttc
    10021 atatgctatt tctgcctgtt caagcgaggg attgtcattt gatttttgaa ctttttctgt
    10081 catattgggg tgtgcaacag acggtgcgcc ctgattgtgt cgcataattt cgtcaatcgc
    10141 aggaatataa catccgctat ccgctgtgtt tgacggacat gaattgttga ggcaggcttt
    10201 gtttttgtca agtccgtaat gatgttcggg attaatctga tttgccgtag gagttgtatt
    10261 aactgccata tttgaagaat tatccattaa gctatgctcc tttatgtttt ggaataagcc
    10321 tcggtaggcg aataaaagca atgatcccct accctgttgt aaattactcc gttgtttgac
    10381 ggaaaaaatg ttggacaggt atctgaaaat ggattaaaat agaaaattga atttccgaca
    10441 gatgaatcgg tattgcccgc caatgcccaa tcggcaatgt tatagtgaat ttcgtcaggg
    10501 cgtatattat aaacattctg aggattatat tgtcctccga cagttgtttt aagacaggta
    10561 aattgatttg gttgttccat tatggcacgg aaatctccgc ccttgctgac tcttgcaaat
    10621 tccccgtttg gaacacgggt tcggtttaca attaccgacg caacggcacg cattccgttt
    10681 tcaccttccc cacctgcctc acattttaaa agccttgcaa gcaattctct tgtactcaat
    10741 gccacctttt ttcaccaacc gttattttat tcaatgatat tttatgcata aattaacttg
    10801 ttgttacaaa gaaaaagctt gcgataaatg tgttattcac gcagacagaa aattctgttt
    10861 tatagaagca actcagacgg tgttcgataa tcttttgaga tgccaactac aacatttatt
    10921 cagattagct gttgctgtct accgctttac ttgcttttgc agggctattt tgccaatcac
    10981 cgaaccatca gataaattgt ttgctttgag taatattatt gattttttat tgacactatc
    11041 atcaatagtg cgtataatga tattggaacc cgaattttta agttggctgg gtaattggta
    11101 ccctgacttc ttaaaaatat tctgggttct ttttttgtct aaataaaaaa atcctgtttt
    11161 ccctgttgtt tctaaagcaa cagtttcttt gattaaatca ttgatgttgt ttttgccgtc
    11221 atcgttcaca agccaatcat cgtattcttc gtccattaaa aatttcagcg tatcgtgtat
    11281 tacactggct ttttcatctg taaaatcata atcttcatca agcgagaatt tttcttttac
    11341 tcctgcggaa agttcttgcc caatctcttg attaactcct gctctgttgg attgtccttc
    11401 attaactttt ccatttttaa cagagtttcg tccgatgtca ttgaattgct tactcttaat
    11461 gttgtattat ccgagtataa gttcaaactg tttagtataa tctctgagta attgtctaaa
    11521 tactttcttg ctatcataat tgttttcctc taatttaatt ataaaatttg cgattttgtt
    11581 gccatcggtt
//
LOCUS       DAOMVE010000050        11539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1321_length_11539_cov_3.795280, whole genome shotgun sequence.
ACCESSION   DAOMVE010000050 DAOMVE010000000
VERSION     DAOMVE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11539
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1321_length_11539_cov_3.795280"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..61)
                     /locus_tag="PLS64_04240"
     rRNA            complement(<1..61)
                     /locus_tag="PLS64_04240"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            678..1715
                     /locus_tag="PLS64_04245"
     CDS             678..1715
                     /locus_tag="PLS64_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRL43075.1"
                     /translation="MFSINTRAGSTNISFSSGTGGSISILLCIALILLYAKCRSNDPM
                     PFGATVTLIKIYSIIEIVIGGLALLAFTGITFLLALVLQGQEVFGYILILCIFAIPLA
                     LVTLIHGIGLLLWSDSIKKGATSVYLKSRGSILLAVSSILSIIVSVASAIASFIFIKE
                     YGPEISAYLHHLAEENPNFNFSGLFDSSDPMNSLFISAAGVVTVLNTVLSAVFYALLA
                     ALAISFHTYIKKYTNGINFGSGQSPEYDAQPNAVPVPPVAPVTSEMPTEGIQPPVQQP
                     IDFQPQSVDFGSMDATSDNKQINTQFENPYMQAVADDMSDKSNTVVCPNCHAQCNNDM
                     KFCGNCGTKLK"
     gene            complement(1805..2362)
                     /locus_tag="PLS64_04250"
     CDS             complement(1805..2362)
                     /locus_tag="PLS64_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43076.1"
                     /translation="MKRILTAILCAGILAVTAVGCSYNDALYKDGNSSSQTSEDSTQP
                     TTSSVKDDKYGNDLDGLVDYFVAKEYLESKDGSIKMDASAIGAKEGKKFAAKYAGSNI
                     IIELYSYDTKKTNSTADEIVKSVKDNGTFSIYGLPDVTAYLSDNGNYLMIYTDTAIDK
                     TNPDKNSDNYKHREQVIKDFKAFKK"
     gene            complement(2661..3809)
                     /locus_tag="PLS64_04255"
     CDS             complement(2661..3809)
                     /locus_tag="PLS64_04255"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HRL43077.1"
                     /translation="MQKKKENFRSLRSVTTFALAAAIITGSIYTVAAAVNYSAETEHA
                     VTSDGVELANGDIVYAGLDENGFTYGMIRAVEISVDYNGDVQTVSLAKGTVSDVLERT
                     GITPAYNEVVEPSLSTPVSDNLTVKVYKGKKLSITADGKTDSVYVPNGNVCDVLNQLG
                     YKLSDDDILSVDKDSNIEDADSITIKRVTYRNETETQSVDFETVKKNSKDVDLGKTKV
                     QTEGKQGEALVTKKCKYVDGKKVSSKTVDTKITKEPVDKVVLMGTKGSNVSNPVGTFT
                     DMNGNTVAYSSVVTGSGTAYTAPAGSLTATGVTAYHGGVAVNPNIIPYGSKLYIVSTD
                     GSFVYGYATAVDTGGALMSGTAIVDCFYNTYDECVSFGRRNVNVYIVG"
     gene            complement(4214..5056)
                     /gene="dinD"
                     /locus_tag="PLS64_04260"
     CDS             complement(4214..5056)
                     /gene="dinD"
                     /locus_tag="PLS64_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HRL43078.1"
                     /translation="MTSIEKYSDRIFENIKQTNEYGQEFWYARELQEVLEYSQWRNFY
                     EAVQRAAVSCENSGYDIDEHFEECEVVRKIGNGANRKVKDIKLSRYACYLIVMNGDSQ
                     KEIISLGQTYFAVKTRQQELSENFDSLSEDAKRLAIRNELKRHNSMLADAAHDAGVVE
                     QRDYAIFQNYGYKGLYGGLGAKEIHNKKGLKKSQKILDYMGSTELAANLFRATQTEEK
                     LRRDNIKGKQEANQTHYHVGKKVRETIKELGGTMPEDLPTPKSSIKEIEQKSQKTITK
                     KKDE"
     gene            complement(5214..6026)
                     /locus_tag="PLS64_04265"
     CDS             complement(5214..6026)
                     /locus_tag="PLS64_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL43079.1"
                     /translation="MEQNSNYRVLSNVCVSCGVRIPDIDFEIKPAMRETYGLFLVTAG
                     KGIFIIDGIEYPVKRGNSVMLFPYSSISVKAYPNIMFEYKWVELTGFEIAVMISRTNF
                     SKSRPVAPEIPVESLFQYFDVCDDSSDTVYAACRTNGKVLILLSYYIQYYPSRKSTET
                     DYIMQARDYIERNFRQVSCTVKSVADKVQIDRTYLYRLFKEETGFSVIEYINNCRIGR
                     ACTMLNNTNVQIKDVALSVGFSDQLYFSKVFRKLKGITPTEYRKKYANETTL"
     gene            6164..7000
                     /locus_tag="PLS64_04270"
     CDS             6164..7000
                     /locus_tag="PLS64_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRL43080.1"
                     /translation="MNYKYEIINYDQNTPAKVMYLNLSSETHKTELHWHREPELVYVI
                     EGQAECPRNGETTLVNEGDFILFNSEDVHLVRPVVGTSVRLVCIQLSFEYMRMFCKSI
                     DSVVFDLSVSPQTKQKLIEVLQEIAETNPNDEYSTLLQIAHVNKIYYLLLKNCTCFKR
                     ATNNPIIPRRDFSYAKTAIAYINENYKREIPLNEISSVVNLSPSYFSKYFKSITRVSF
                     SEYLANLRLENAINDMLDKNSTVSAAALENGFANVKSFITQCKKVYGLTPAQYKKKLL
                     NR"
     gene            complement(7066..7914)
                     /locus_tag="PLS64_04275"
     CDS             complement(7066..7914)
                     /locus_tag="PLS64_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRL43081.1"
                     /translation="MKFEMNLKIEKPRYFTVSDITYAQVDAWFGHTTRDLKLDLIYPK
                     LKNKKYPCIVWFCGGGWIRLDKSAHFAYLAKLALEGFAVASVEYRTSNEGAYPMPLED
                     VKAAIRYIKAHADRYNIDENKIGVAGESAGGYLAAMCALDNDKALDVGNYLDCSSEVQ
                     ACCAFYPPTDVSTFPYESPLTSAASMESLMLGMNVALNKEKAMKCCPVSYVTPSAPPF
                     MIFHGTDDSTVPFSQSAELHDLLEKNGCDVTLVAINGAEHADIFFAQEKLWDMVAKFF
                     KDKLYQ"
     gene            complement(7929..8858)
                     /locus_tag="PLS64_04280"
     CDS             complement(7929..8858)
                     /locus_tag="PLS64_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRL43082.1"
                     /translation="MKKLIPVLAMIMLVPFVICSCFVQQAKSMKLSYEEIEFHVGDTK
                     HISVTIEPEGADAGKISWSSSDNKIVTVDDGTITGKSKGTAVVTAESESGLKKLCNVT
                     VLDKEIESVTLSESSTSVKQGGKIQLEAKVKPVDAPSDNLVWSSSDPKVASVDGNGMV
                     LGESEGVVTITCESANGKKATCTVTVKGNNMNPTESYTKSTEPTKSDDAKRHEGSNSS
                     SSKSDESRNNGFIFPHSSQRKLTVDEVTGLGGDVAQDAINEIYARHGYVFKTKSIQKY
                     YESRSWYHKNYNFSESDLSEIESYNIGLLRKYN"
     gene            complement(8947..9600)
                     /locus_tag="PLS64_04285"
     CDS             complement(8947..9600)
                     /locus_tag="PLS64_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRL43083.1"
                     /translation="MEHTINACGHANSTVHENMQSLIEQSGSVRKYAKDEIIYLQGDT
                     AQSFCYIKKGSVKVYMNSVDGNEKTLNTARHGEIIGEGAFFDKKPRVSSAKALTNTEV
                     IMIDKKRLTDIIAQHPTLAFELLEILANRIRLLSSQLDSMTFMQADARIAGLLLESEH
                     DGIVAITHEEIANTVGVSRVTVSKTLSRFAKSGYISTEYRRILIKSREGLEKLVNES"
     gene            9709..10974
                     /gene="leuC"
                     /locus_tag="PLS64_04290"
     CDS             9709..10974
                     /gene="leuC"
                     /locus_tag="PLS64_04290"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRL43084.1"
                     /translation="MGMTMSQKILAAHAGLSEVKAGQLIEAKLDMVLGNDVTSPVAIN
                     VFEENGATSVFDNTKIAMIMDHFTPNKDIKAATQVKQVRTFADKYDIKNYRDVGQMGI
                     EHALLPEQGLVGPGCLCIGADSHTCTYGALGAFSTGVGSTDMAAGMISGKAWFKVPSA
                     IKFNIVGKPQGFVSGKDVILHIIGKIGVDGALYKSMEFTGEGLKYLNIDDRLCIANMA
                     IEAGAKNGIFPVDDITREYCNGRYQGTPVEYTADEDAVYDEEYTIDLSTLRPTVAFPH
                     LPENTRTVDEIGEKEVKIDQCVIGSCTNGRISDLRAAASILKGKKIAKNVRCIIFPGT
                     QQIWLDAMHEGLFDIFIEAGAVVSTPTCGPCLGGHMGILAAGERAISTTNRNFVGRMG
                     HVDSEVYLASPAVAAASAVAGYIIDPEKV"
     gene            10994..11473
                     /gene="leuD"
                     /locus_tag="PLS64_04295"
     CDS             10994..11473
                     /gene="leuD"
                     /locus_tag="PLS64_04295"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRL43085.1"
                     /translation="MNAKGTVHKFGDNVDTDVIIPARYLNTASHKELAAHCMEDIDAD
                     FVNKVKEGDIMVAEKNFGCGSSREHAPIAIKASGISCVIASTFARIFYRNSINIGLPI
                     LECDEAAKDIKEGDTVSVNFDTGVITNETTGKTYQAEPFPEFIQNIIKKGGLINSIM"
BASE COUNT         3176 a         2550 c         2160 g         3653 t
ORIGIN      
        1 cttgcatgtg ttaggcacgc cgccagcgtt cgtcctgagc caggatcaaa ctctctaaaa
       61 tttgtatctc aacagccttt cagctgttca aatcaatttc agagctttaa tcgctcattg
      121 acgaatatgt cactgactat atccgtaatt cctgatgctt tttagtgtac atctcactgt
      181 aattctttga gctttcctca aagtcattac gggtttcttt actattttct cgttgttcaa
      241 ttttcaaggt cctatgttca ctttgcacaa gaaaaacttg attctctctt ttgttttaag
      301 aggatttgtt attttccttc gcggaacatt ctctattata tacctttttc cagaaaagtc
      361 aacccctttt ttcaaaaaaa ttcaattttt tcagaaaaag ttttagcctt gtggttttgc
      421 caacaaattt ctttgtttta tcacaacata ttgtggttgt ctaaaataca cctttttaca
      481 attttcaaaa tcctattgta ttttcccttt ctataatata aaatagaaat acaaattaat
      541 ttttcggagg aaaatttatg attaattcca ttccctatta tccgtatcaa cagaatgtgc
      601 agatattgaa aggattcttt tccaaaccgc ttacattaat atgtgcaata gtctccggcc
      661 ttacatgtat tttcggaatg tttagcatta atacaagagc aggttctacg aacatatctt
      721 tctcgagcgg cacgggcggt tcaatttcaa ttcttttatg tattgctctt atcctgcttt
      781 atgcaaagtg ccgttcaaat gatccaatgc cgtttggcgc aacggttact ctcatcaaga
      841 tatattctat cattgaaata gtaatcggcg gacttgcatt gctcgccttt accggaataa
      901 cttttcttct tgcattagtt ttgcagggac aggaagtatt cggatacatt cttatcctct
      961 gcatctttgc tattcctctt gcacttgtaa cactcataca cggtatcgga ttgcttttat
     1021 ggtctgattc aattaagaag ggtgcaacat cggtttatct caaaagcaga ggttcaatac
     1081 ttcttgccgt gtcttctatt ctctctatta tagtaagcgt tgcatccgca attgcttcat
     1141 tcatctttat taaggaatac ggtccggaaa tttcagcgta tctgcatcat ttggctgagg
     1201 agaatccgaa ctttaatttt agcggtctat ttgattcgtc agatcccatg aattcattgt
     1261 ttatttccgc tgcgggggtt gtaacggtat taaatacagt actgtctgct gtattttacg
     1321 ctttgcttgc agcacttgct atttcttttc atacatatat aaagaaatac acaaacggaa
     1381 ttaacttcgg ttcaggtcag tctcctgaat acgacgctca gccaaacgct gttcccgtac
     1441 ctcctgttgc tcctgtaaca agtgaaatgc cgacagaagg tattcagccg cctgttcagc
     1501 aaccgattga ttttcagccc caaagcgttg attttggctc aatggatgca acttccgaca
     1561 acaaacagat taacactcag tttgaaaatc cttatatgca agctgtcgca gacgatatga
     1621 gtgataaatc aaatacggtt gtttgcccga actgtcatgc acaatgcaat aacgatatga
     1681 agttctgcgg aaactgcggt acaaaactca aataaaaaac gctatacata tatatagaaa
     1741 aaagcggaga ttgaagttga atcaatctcc gctttattgc gtcacaatta tttacatata
     1801 tatgttactt tttaaaagcc ttgaaatcct tgataacctg ctcacgatgc ttatagttat
     1861 cactattctt atcgggattt gtcttatcaa tagcagtgtc tgtataaatc ataagatagt
     1921 tgccgttgtc cgaaagatag gctgttacat cgggaagtcc gtagatgctg aatgtgccgt
     1981 tgtccttaac agactttaca atctcatcgg ctgtgctgtt cgttttcttt gtatcataag
     2041 agtaaagctc aatgataata tttgaacctg catactttgc agcaaacttt ttaccttctt
     2101 tggcgccgat agcggacgca tccattttaa tgctgccgtc tttactttca aggtattcct
     2161 tagctacaaa ataatcaaca agtccgtcaa ggtcattgcc gtatttatca tccttaaccg
     2221 aagatgttgt aggctgagtg ctgtcctcgc ttgtctgtga agaactgtta ccgtccttat
     2281 aaagtgcatc attatagctg caaccaactg cagtaaccgc aagaatgcct gcacataaaa
     2341 tcgctgtaag aattctcttc ataatatttt tcctttcggt tcattctttc gttctgtcat
     2401 aagtacagta tattataccg catattttcc gataaatcaa tataaatttc aaaaacaata
     2461 ggcagatgcc gaagcgtctg ccctgattat gtcgtaacat tattaatatc gtaagatttg
     2521 attatataga actgcccttt caaattaagt gtgatgcact caccatacag agttaattct
     2581 atcaccaagt caagctcggg aattcatagc ttagattata tcaaactatc tacacgaact
     2641 aaaattaaca cgagctaaaa ttaacctact atatatacat ttacatttcg tctgccaaag
     2701 cttacgcatt catcatatgt gttgtagaaa cagtcaacaa ttgcagttcc cgacatgagc
     2761 gcaccgcctg tgtcaacggc tgttgcgtag ccgtaaacaa aactgccgtc tgttgataca
     2821 atgtaaagct ttgagccata cggaatgatg ttcgggttga ctgccacacc gccgtgataa
     2881 gccgttacgc ctgttgccgt aagtgaacct gcgggtgctg tgtacgctgt acctgaacct
     2941 gtaacaaccg atgaataggc aacggtgttg ccgttcatat ccgtaaatgt gccgacagga
     3001 ttgctgacat ttgaaccctt tgtacccata agtacaacct tgtcaacagg ctcttttgta
     3061 attttggtgt ctactgtttt tgaagatacc tttttgccgt caacatactt gcacttcttt
     3121 gttacaagag cttcgccctg tttgccctca gtctgaacct ttgttttgcc aaggtcaaca
     3181 tcttttgaat ttttcttaac ggtttcaaaa tcaaccgact gagtttcggt ttcgttcctg
     3241 taggtcacac gcttgattgt gatgctgtct gcgtcctcaa tgttgctgtc cttgtcaacg
     3301 ctgagaatat cgtcatcgga aagcttgtag ccaagctgat tgaggacatc gcaaacattg
     3361 ccgttcggaa cataaacgct gtcggttttg ccgtcagccg taattgaaag ctttttgccc
     3421 ttgtaaacct taacggtgag attatccgaa accggagttg aaagtgacgg ctcaaccacc
     3481 tcgttatatg cgggagtgat gcctgttctt tcaagcacat cggaaaccgt accttttgcg
     3541 aggctgactg tctgaacatc gccgttatag tcaacgctga tttcaacagc cctaatcatg
     3601 ccgtatgtaa atccgttttc atcaagtcct gcataaacga tatcgccgtt tgcaagctca
     3661 acaccgtctg atgttacagc atgttctgtt tctgcactgt agttcacggc agcagctacc
     3721 gtgtaaatcg aacctgtaat aattgcagct gcaagtgcaa aagtcgttac ggaacgcagg
     3781 gaacgaaagt tctctttctt tttctgcata ataaatttct cctgacttgc aattttctca
     3841 ggattgattt tgcatagaaa attgcatatt taattcataa accttcacta aagagccgtc
     3901 ttgctcttta caggcacaca ttataggact gtgttttgtg aatgtcaaac caatcaaaag
     3961 taaatttttt gtgcaatttc acaagacttt cgcctttatt ttctattttt gcaatataat
     4021 cttgtttcct atcgggaatt atcctgcaaa tttatatgca atttgcaaga aattcacttt
     4081 taccgtttcg ttaccgattt gttactgttt tgtaaccact ttctcataga gaattttatc
     4141 gctttaaaca gccgttttta atgctttttt agcaacaaaa acgaggtcaa atcgacctcg
     4201 ttttctgctg tatttattca tcttttttct ttgtaattgt tttttgtgat ttctgttcta
     4261 tttctttaat actgctcttt ggagtgggca aatcttccgg catagtaccg ccaagttcct
     4321 ttattgtttc acggactttt ttgccaacat gataatgggt ttgattagcc tcctgcttgc
     4381 cctttatatt atctcttcta agtttttctt cagtttgagt tgctcggaat aaattagcag
     4441 ctaattcagt gctacccata taatccaaaa ttttctgact tttctttaag ccctttttat
     4501 tatgtatttc tttcgctcca agtccgccgt aaagcccttt atatccataa ttttgaaata
     4561 tagcataatc cctttgttct acaacacccg catcatgcgc tgcatctgcc aacatagaat
     4621 tatgtctttt taattcattt ctgattgcta atctttttgc atcttcagat aaggaatcaa
     4681 aattttcaga aagttcttgt tgtcttgttt ttacagcaaa ataagtttgt ccaagggata
     4741 taatttcctt ttgagaatca ccgttcataa cgattagata gcaagcgtaa cgagataatt
     4801 ttatatcttt tactttccta tttgcaccat tgcctatctt tcttacaact tcgcattctt
     4861 caaaatgttc gtctatatca taaccgctgt tttcacatga tacagccgct ctttgaacag
     4921 cttcataaaa atttcgccat tgcgaatatt caagcacttc ctgaagttct ctcgcatacc
     4981 aaaattcttg accatattca ttagtctgtt tgatgttctc aaatattcgg tcgctatact
     5041 tttcaatact agtcatgact atcctccttt atgcaaagta tacaaaattt tcgttttttt
     5101 gcaacatttt gctggcgtca gcaaaatgtt attattcaaa ttactaatct taaatcagct
     5161 attattatat aataattata atgtaactca cattaaaaat caacactaaa gttttataat
     5221 gttgtttcat ttgcatactt tttgcggtac tccgtagggg tgattccttt cagctttctg
     5281 aaaacctttg aaaagtataa ctgatcggaa aagcctaccg agagggcgac atccttaatc
     5341 tgaacattcg tgttgttaag cattgtgcag gctctgccga ttcggcagtt gttgatgtac
     5401 tcgataaccg aaaatcccgt ttcttcctta aaaagtctgt aaaggtaagt gcggtcaatc
     5461 tggactttgt cggcaacgct tttaaccgtg catgaaacct gccgaaagtt tctttctata
     5521 taatctctcg cctgcataat gtagtcggtt tcggttgatt tccgactcgg ataatactga
     5581 atatagtatg acagcagaat cagcaccttg ccgtttgtgc gacaggcggc ataaaccgtg
     5641 tcgctgctgt catcacagac atcaaaatat tgaaaaagac tttcaacggg aatttcgggg
     5701 gcaacaggac ggctttttga aaaattggtt cggcttatca ttacggcaat ttcaaacccc
     5761 gtgagttcaa cccatttgta ttcaaacata atattcggat aggccttgac gcttatgctt
     5821 gaatacggaa aaagcataac cgaattaccc cttttcacgg gatattcgat gccatctata
     5881 ataaaaattc ccttgcctgc cgtaacaaga aacagaccgt aggtttcacg cattgcgggt
     5941 ttgatttcaa aatcaatatc aggaatcctc acaccgcacg aaacacaaac atttgacagt
     6001 actctgtaat tactattttg ttccatataa tcgcctcaaa tacaacattt gtccataata
     6061 tcataacata tctatataga ttttcgtgct ttttcacgct ataattatgt tattatacaa
     6121 taattttgca tttttgacaa taggtgattc ggagtgaaaa attttgaact ataaatacga
     6181 aataataaat tatgaccaaa acacacctgc aaaggttatg tatcttaacc tttcgtcaga
     6241 aacacacaag actgaacttc attggcacag agagcctgag cttgtctatg tgattgaagg
     6301 tcaggcagaa tgtccgagaa acggcgaaac aacgcttgta aacgaggggg attttattct
     6361 tttcaacagt gaagatgttc accttgttcg gcctgttgtc ggaacaagcg tccgccttgt
     6421 gtgcattcag ctttcgtttg aatatatgcg aatgttctgc aagtcaattg acagcgttgt
     6481 ttttgacctt tctgtttctc ctcaaacgaa gcagaagctt attgaagttc ttcaggaaat
     6541 tgccgaaaca aatcccaatg acgagtacag cacacttctt caaattgctc atgtcaataa
     6601 gatttactat ctgctcttga agaattgcac ctgctttaag agagcgacca acaatccgat
     6661 tattccgcgc agagattttt catatgcaaa aacagcaatt gcatatatta atgaaaacta
     6721 caagcgtgaa attcctctta acgagatttc atcggttgta aatctttctc cgtcttattt
     6781 ttcaaagtac ttcaaaagca tcacccgtgt gagcttttcg gagtatcttg caaacctccg
     6841 acttgaaaat gcaatcaacg atatgcttga taagaactcg acggtttctg ccgctgcact
     6901 tgaaaacggc tttgcaaatg taaagtcatt catcacacag tgcaaaaagg tctacggtct
     6961 tacaccggcg cagtataaaa agaaacttct caacagataa aattttaaag gctcagaact
     7021 ttgtacaggt tctgagcctt tttgcatacg gcatacagcc gtttcctatt gataaagttt
     7081 atccttgaaa aatttcgcca ccatatccca cagcttttcc tgtgcaaaaa atatgtcggc
     7141 gtgttccgct ccgttaattg caaccaatgt gacatcgcaa ccgtttttct caagcaaatc
     7201 gtgcaattcg gcactttgcg aaaaaggaac ggtggaatcg tctgttccgt gaaaaatcat
     7261 aaacggcggt gctgacgggg ttacatacga aacggggcaa cacttcattg ccttttcctt
     7321 attgagtgca acattcattc cgagcatgag cgactccata cttgccgccg aagttaacgg
     7381 cgattcatat ggaaatgttg aaacatctgt cggcggataa aaggcacagc aagcctgtac
     7441 ctcgcttgaa cagtcaagat aatttccgac atcaagagcc ttatcattat ctaaagcgca
     7501 catggcggca agatagcctc ctgcggattc gcctgcaaca ccgattttgt tttcatctat
     7561 attataacgg tctgcgtgag ccttgatgta gcgaatagcc gcttttacat cctccaacgg
     7621 catcggatat gccccttcat tgcttgtgcg atattcaaca cttgcaaccg caaagccctc
     7681 gagtgccaac tttgcaagat aagcaaaatg ggcagattta tccagtctta tccaaccgcc
     7741 accgcaaaac cacacaatac acgggtattt tttgtttttt aattttggat aaatcaagtc
     7801 gagtttcaag tcccttgtgg tgtgtccgaa ccatgcgtca acctgtgcat aggttatgtc
     7861 ggatacggta aaatatctcg gtttttcaat tttaagattc atttcaaact tcataatatt
     7921 atcccttatc agttgtattt gcggagcaag cctatattat atgactcaat ctccgaaagg
     7981 tcgctttcgc tgaaattgta gtttttgtga taccagctcc ggctctcata gtatttctga
     8041 attgattttg ttttgaaaac atatccgtga cgggcataga tttcattgat tgcatcctgt
     8101 gcaacatctc cgccgagtcc cgtaacttca tctacagtaa gttttctttg tgacgaatgc
     8161 ggaaatataa aaccgttgtt acggctttcg tctgattttg acgaagaact gtttgaacct
     8221 tcatgcctct tagcatcatc actctttgtc ggctctgttg attttgtgta actttcggtc
     8281 ggattcatat tgttgccctt tacggtaacg gtacaggttg cctttttgcc gtttgcggat
     8341 tcgcaggtga ttgttacaac gccttcgctt tcaccaagaa ccatgccgtt gccgtcaaca
     8401 cttgcgacct tcggatccga tgacgaccac acaaggttgt cggacggagc gtcaacaggt
     8461 ttaacctttg cctcaagctg aattttacct ccctgtttta cggaagttga gctctcgctc
     8521 agagtcacgc tttcaatctc tttgtcaaga acggtcacat tgcaaagttt tttaagtccg
     8581 ctttcgctct ctgccgtcac aacggctgtt ccctttgatt tgcctgtgat tgttccgtca
     8641 tcaacggtca caattttatt gtccgaagat gaccagctta tttttcccgc atctgcaccc
     8701 tcgggttcaa ttgttaccga aatatgctta gtatcgccca catgaaattc aatttcttca
     8761 tagctcaatt tcatgctctt ggcttgttgt acaaagcatg agcaaatcac aaacggcaca
     8821 agcataatca tcgccaaaac gggtattaat tttttcacaa atactccccc tgtccttaat
     8881 atatgtaaaa tccttattct tatctgtatt ctaacctatt tatatacttt tgtaaaccac
     8941 agccgatcaa ctttcattca caagcttttc aagaccttct ctgctcttta taagtattct
     9001 tcggtactcg gtagaaatgt atcccgattt tgcaaatctg ctcagagttt tgctgaccgt
     9061 taccctcgat acgcccaccg tgttggcaat ttcttcatgc gtaattgcaa caatgccgtc
     9121 atgttcgctt tcaagcaaga gccctgcaat acgggcgtct gcctgcataa aggtcattga
     9181 gtcaagctgt gatgacaaaa gtcttattct gttggcaaga atttcaagca attcaaacgc
     9241 aagcgtaggg tgctgtgcga taatgtcggt caatcgcttt ttgtcaatca ttataacttc
     9301 ggtatttgta agtgccttgg cggaactgac tctcggcttt ttgtcaaaaa aagcaccctc
     9361 accgataatc tcgccgtgtc ttgccgtgtt gagagttttc tcgttgccgt caacggaatt
     9421 catatacacc tttacactgc cctttttgat gtagcaaaag ctttgcgccg tgtcgccctg
     9481 aagatagatt atctcatctt ttgcgtattt tctgacgctc cccgactgtt caatcagcga
     9541 ctgcatattc tcatgaacag tgctgtttgc gtgtccgcag gcattaatcg tgtgttccat
     9601 aattcaccgc cctaataatc cgatttattt ccttaattgt aacatagttt acaatctttt
     9661 tgcaaggtgt atgcaataat tatcttaaaa gaaaaggagt gatttattat gggtatgact
     9721 atgtcgcaga agattcttgc ggctcacgca ggactcagcg aagtcaaggc aggtcagctt
     9781 attgaggcaa agctcgatat ggttctcggt aacgatgtaa catctcccgt agccattaat
     9841 gtattcgagg aaaacggcgc tacctcagta tttgacaaca caaaaatcgc aatgattatg
     9901 gaccacttta cacctaataa agatatcaag gcagcaacac aggttaaaca ggttcgcaca
     9961 tttgcagaca aatatgatat caaaaactac agagatgtag gtcagatggg tattgaacac
    10021 gcacttctcc ccgaacaggg acttgtagga ccgggatgtc tttgcatcgg tgctgactca
    10081 cacacctgta catacggcgc actcggcgca ttctcaacag gtgtcggctc aacagatatg
    10141 gcggcaggta tgatcagcgg taaggcttgg ttcaaagttc cgtcagctat caaattcaac
    10201 atcgtaggca agccacaggg ttttgtaagc ggtaaggatg ttattctcca catcatcggc
    10261 aaaatcggcg ttgacggcgc actttacaag tcaatggaat tcacgggcga aggtcttaaa
    10321 tacctcaaca ttgatgacag actttgcatt gcaaacatgg caattgaggc aggcgcaaag
    10381 aacggtatct tccctgttga cgacatcacc cgtgaatact gcaacggcag atatcagggt
    10441 acccctgtcg aatacacggc agacgaggac gctgtttatg acgaggagta cacaatcgac
    10501 ctttcaactc tccgtccgac agttgcattc ccgcatcttc ccgaaaatac aagaacagtt
    10561 gatgaaatcg gcgaaaaaga agttaagatt gaccagtgcg taatcggttc atgcacaaac
    10621 ggcagaattt cagacctgcg tgccgccgca agcattctca agggcaagaa gattgcaaag
    10681 aatgttcgtt gcatcatctt ccccggcaca cagcagattt ggcttgacgc tatgcacgaa
    10741 ggtctttttg atatcttcat tgaggcaggc gcagttgttt caacaccgac ctgcggtccg
    10801 tgtcttggcg gtcatatggg tattcttgcc gcaggcgaaa gagcaatctc aacaactaac
    10861 cgtaactttg tcggcagaat gggtcatgtt gacagcgaag tttatcttgc aagtccggct
    10921 gttgccgccg caagtgctgt cgcaggctac atcattgacc ccgaaaaggt ataagaaagg
    10981 caggtaacaa acaatgaacg caaaaggtac tgtacacaag ttcggcgaca atgtggatac
    11041 cgatgtaatt atccctgctc gctaccttaa caccgcatct cacaaggagc ttgcggctca
    11101 ctgcatggag gacattgacg cagatttcgt aaacaaggtt aaagagggcg acattatggt
    11161 tgccgaaaag aactttggct gcggttcttc ccgtgaacac gcaccaatcg caatcaaggc
    11221 aagcggaatt tcatgtgtaa tcgcatcaac ctttgcaaga attttctatc gtaactcaat
    11281 caacatcggt cttccgattc ttgaatgtga cgaggcggca aaggatatca aagagggcga
    11341 cacagtttcc gtaaactttg acacaggcgt catcaccaac gaaacaacag gcaaaacata
    11401 tcaggctgag ccgttccccg agttcattca gaacatcatc aaaaaaggcg gtctcatcaa
    11461 ctcgattatg taatccgttg attttgattt ataattataa cagaacaaca aacggcagac
    11521 ttgtaaataa gtctgccgt
//
LOCUS       DAOMVE010000051        11410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1343_length_11410_cov_3.006957, whole genome shotgun sequence.
ACCESSION   DAOMVE010000051 DAOMVE010000000
VERSION     DAOMVE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1343_length_11410_cov_3.006957"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(710..1876)
                     /locus_tag="PLS64_04300"
     CDS             complement(710..1876)
                     /locus_tag="PLS64_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HRL43086.1"
                     /translation="MQVKISDSIKYIGVDDKTIDLFESQYLVPNGISYNSYLIVDEKV
                     AIMDTVDKRKTDEWLENLDRELNGRTPDYLVISHLEPDHASNIKVVSEKYPSMKLVGN
                     AKTFSMLPQFFPDFDFADKTVTVKEGDELELGSHKLTFIMAPMVHWPEVMVSYESAEK
                     VLFSADGFGTFGALDAQEDDWACEARRYYFNICGKYGVQVQNLLKKAAKLDIRKICPL
                     HGPVLDENLGWYIGLYDTWSKYEPETDGVFIAYCSVHGNTAKAAEKLCEIIKSKTDKK
                     VSIADLSRTDLAEDIEDAFRFSRMVVIAPSYDGGVFPIMNDFLHHLKIKGYKNRKVAM
                     VENGSWAPSAAKTMRTYLEEMKNVEICPTVVTIRSAMKEENIPQLEQLSDEILG"
     gene            2097..2357
                     /locus_tag="PLS64_04305"
     CDS             2097..2357
                     /locus_tag="PLS64_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRL43087.1"
                     /translation="MEVKYSKAALKFLAKSDKATVNRIRIGIEGLKINPPKGDIKLMQ
                     GFDDGRQRLRIGKYRIIFKRLLDGTYEIIYIMDIGSRGDIYK"
     gene            2373..2594
                     /locus_tag="PLS64_04310"
     CDS             2373..2594
                     /locus_tag="PLS64_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43088.1"
                     /translation="MSTKTAEIAKMIDMLPDSDKELAYELIKKLVLAWDPDYTKVTEQ
                     EYKDITEAENGDFVSEDDIDWDNLSRYAN"
     gene            complement(2799..5018)
                     /locus_tag="PLS64_04315"
     CDS             complement(2799..5018)
                     /locus_tag="PLS64_04315"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRL43089.1"
                     /translation="MNRLMTFDKYRVFETEFAGRPLKVETGKMTQLANGACLVHYGDT
                     TVHVAVTASEKPREGVDFFPLSVDYEEKMYAVGKIPGSYLKREGRPSEKAILTSRVID
                     RPIRPLFDKSMRNDVSIVCTVMSVDPDCQPEIVAMIGASIAISISDVPWNGPISGCSV
                     GMIDGEYIINPTEEQRKVSQMATTVASTSTRIAMIEAGANCVSDDDMYNAIMAGHEAN
                     QKIISFIEEIKAEIGKPKFEFASLEPDHDMFEAIKAFAEEDVKVALDTDDKRIRDERL
                     KPIYEAVHAKFDEIYPESEALIDECLYKTQKFIVRRWLLDEQKRVDGRGMDDIRPLAS
                     EVGVIPRVHGSGMFTRGQTQVLTIATLGPVSDKQLLDGIDGETEKRYIHHYNFPSYSV
                     GETKPSRGPGRREIGHGALAERALVPVIPSVEEFPYALRLVSEVLSSNGSTSQGSICG
                     STLALMDAGVPIKAPVAGISCGLITEGDRWMTMVDIQGLEDFFGDMDFKVAGTHDGIT
                     AIQMDLKISGLTPEMVKEALAKTHKARNYILDEVMLKAIAEPREELSKYAPKMFTTTI
                     PADKISEVIGKSGKVIKEIQAECEVKVDIEEDGELGQVFVSGIDSDKCKRAIEIINTI
                     AVDPEPGAMYLGKVTRIMDFGAFVEIAPGKEGLVHISQLDVKRTENVTDVVNVGDIIR
                     VKVMEIDDKGRLNLSRRQVLIDVDGLVPENDIDAERAARPRRPHNDRRGGFNRNNRK"
     gene            complement(5245..5508)
                     /gene="rpsO"
                     /locus_tag="PLS64_04320"
     CDS             complement(5245..5508)
                     /gene="rpsO"
                     /locus_tag="PLS64_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRL43090.1"
                     /translation="MLKEEKQAIMAEYATHEGDTGSPEVQIALLTKRINDLTEHLKVH
                     KKDHHSRRGLLKMVGQRRSLLAYLTKVDIERYRSIIARLGIRK"
     gene            complement(5648..6040)
                     /locus_tag="PLS64_04325"
     CDS             complement(5648..6040)
                     /locus_tag="PLS64_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL43091.1"
                     /translation="MPKRKCSNIDASVGLRMRYYRIQCGLTQQQVADALKINRTTYTK
                     YETGVSEPSHELLGKIVKMFGIDYNAILDNRDYFERRVADGDMTLNVLTLSERAIIAR
                     YRSMSKEDQKKICEYIDMLYNEKAKKIL"
     gene            6408..6483
                     /locus_tag="PLS64_04330"
     tRNA            6408..6483
                     /locus_tag="PLS64_04330"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6441..6443,aa:Thr,seq:cgt)
     gene            complement(6675..7535)
                     /locus_tag="PLS64_04335"
     CDS             complement(6675..7535)
                     /locus_tag="PLS64_04335"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HRL43092.1"
                     /translation="MKNFNQSSLARFFTRFPKLLFAGLMYSIPFAVFSGIFILISFLS
                     GFNNVILWSLGIIPAMPFYSGLVMVIRKISVEKEDVNVFKTFVQAFRENLKKSIFNGF
                     VAYLIVACSFFAILYYGTLAQTDIVYGSVFTIYIVFSIAMLIMLFYVPLLTITYDLRL
                     RDIYKNSLLLIFGKILRNIVALVLVVIVSFAVFLAVIYAKGAMLVVAVALSVIFYPMI
                     ATYIIVSVIAKGVQESVGYFTGDNVQYVLTEEEVQRETEAVENANSDDDYIFVNGRMI
                     KNPAKTNTKE"
     gene            7902..9257
                     /locus_tag="PLS64_04340"
     CDS             7902..9257
                     /locus_tag="PLS64_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL43093.1"
                     /translation="MKKSTIKRAAALAVAGMMTATALAGCGGSSSSGSSKDDSSAAGN
                     AKNGKVYYLNFKPEQDKDWQALAKKYTDETGVEVTVKTAAEGTYESTLTAEMDKDNAP
                     TLFQVNGPVGLANWKDYCADLSSSEIYNQLTSKDYALEEDGKVYGIAYVIETYGIIYN
                     KTLLQKYCDSDFASVKKIEDINSFEKLKTVADEIQKNKDKLGVKGAFTSAGMDSSSDW
                     RFKTHLANLPIYFEYKDKGIKSTDAIEGKYLENYKNIWDLYITDSTCDPADLASKKGT
                     DAETEFTSGEAVFFQNGSWEYSTVTKAGMKDDELGMLPIYIGAGDEANQGLCTGSENY
                     WCVNNKASADDQAATLEFLNWCVTNKTGTSALADDMGFVIPFKNAKEATNVFVKIDNE
                     LTAAGKTPVSWNFTTMPSEDWKNGVGTALTQYAAGTGDWDAVKTAFVQGWKTEYAKAN
                     G"
     gene            9344..10222
                     /locus_tag="PLS64_04345"
     CDS             9344..10222
                     /locus_tag="PLS64_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005332727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRL43094.1"
                     /translation="MFRKPKRKIYFLIFVLPTLAAFLIGFVYPFFNGIYLSFCKFRTT
                     SDAVFIGFGNYVKAFQNEGFLHSFWFTVLFVIVSVILINLIAFFVAYALTLGIRGSNV
                     FRTVFFVPNLVGGIILGYIWQYIFNGILQYYGTNLALNANFGFWGLVILMCWQQAGYM
                     MIIYIAGFQSVPHDLYEAAKIDGAGKKQLLTNVTLPMMMPSITICTFLTLTNSFKLFD
                     QNLALTGGAPGIVSQGSGTMVYQTEMMALNIYNTFYANQNSRGVGQAEGVIFFVIVVI
                     IALIQLYFTRRKEVQQ"
     gene            10222..11100
                     /locus_tag="PLS64_04350"
     CDS             10222..11100
                     /locus_tag="PLS64_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRL43095.1"
                     /translation="MAKVAAKAKDRPVKKKQTVGMKILTILFSIVSVFYVAPVFIVLM
                     NSFKSNAAISRAIFEFPNANSFQGFQSYMTGMFHGNYPFWKSIVWSFFITIGGVVLIL
                     FCTSMCAWYLQRSNSRICKIIYYFCIFSMVIPFQMVMFTLSKTADSLKLTTPWGILII
                     YLGFGAGLAVFMFSGFVKSIPVEIEEAADVDGAGPLRTFFGIVLPIMKPTFISVAILE
                     TMWIWNDYLLPYLILDMDYKTIPIHIQYLNGSYGHTDTSAIMALIVLSIVPIIIFYFA
                     CQKYIIEGVVSGAVKG"
BASE COUNT         3365 a         2294 c         2225 g         3526 t
ORIGIN      
        1 aaccaatttt tcacgatatt taacaacata aggctgacaa tattaccaat cgacaatctt
       61 ataacaaatg atgtgcatct ttatattttt cactgtataa ccgcattcta caagataaat
      121 gacgcataac aaacaagatt tcagcaaaat atttttgatt cggacaaaaa taatccgcct
      181 ccgatcgaaa tcaaaggcgg aaaatgatta tttttttaaa ttgaaaactt gcttgaatca
      241 tattgataca ggttgtgtta taaatccttc gcaaagcaaa tggttgccga aaagtgaaat
      301 gaccgacaac aagcgtcggt cattttcggg gtatattggg ttatagaagg aatatatcaa
      361 tatgaatgaa cgaaataagc attgaataca ggcagaagaa agaacttttc acgactaatt
      421 tcaaaactcc aaaacaaatt acctaaaaac gaatctgttt caatgcttat tccgtgggca
      481 tcaactttag ctttattgta caacaaaaga gttttaaatt caattgcatt tgcaacggaa
      541 ttaaatcttt gatgtaaaat atcctttgga atttttatct aattgttaca aaaataacaa
      601 tctcaacctt tgcagaacaa aattcgccat cttttaggtg taattattga agctataaat
      661 atatatttat atccttttat tattgcgttt atgtcaattt attatgatat tatccaagaa
      721 tttcgtcaga aagttgttca agctgaggaa tattttcttc tttcatagca gaacggattg
      781 tcacaacagt aggacaaatt tcaacatttt tcatttcttc aagatatgtt ctcattgtct
      841 ttgctgccga aggagcccaa gagccgtttt caaccatagc aacctttctg ttcttgtagc
      901 ccttgatttt aaggtggtgc aggaaatcgt tcattatcgg gaacacaccg ccgtcatagc
      961 tcggtgcgat aacaaccatt ctgctgaaac ggaatgcgtc ctcaatatcc tcggcaaggt
     1021 ctgtgcgtga aaggtcagca atcgaaacct ttttgtcggt ttttgatttg atgatttcac
     1081 aaagcttttc ggctgccttg gctgtgttgc cgtgaaccga acaatatgca atgaacacac
     1141 cgtcggtttc aggctcatat ttgctccatg tatcgtaaag accgatgtac caaccaaggt
     1201 tctcgtcaag tacaggaccg tgaagcggac agattttccg aatatcaagc tttgcggctt
     1261 ttttgagaag attctgaacc tgaacaccgt acttaccgca gatattgaag taatatcttc
     1321 ttgcctcgca agcccaatcg tcctcctgag cgtcaagtgc gccgaatgtg ccgaagccgt
     1381 ccgcagagaa aagaaccttt tccgcacttt catatgaaac cataacctca ggccaatgaa
     1441 ccatgggagc cattatgaat gtaagcttgt gcgaaccgag ttcaagttcg tcaccctctt
     1501 tgactgtaac ggttttatcg gcaaaatcaa aatccggaaa aaactgtggg agcattgaaa
     1561 aagtctttgc attaccaaca agcttcattg acggatattt ctcggaaaca accttaatat
     1621 ttgacgcatg gtcgggttca aggtggctga ttacaagata atcgggagtt ctgccgttaa
     1681 gctctctgtc aagattttca agccattcgt cagtctttct cttgtcaacc gtatccataa
     1741 ttgcaacctt ttcgtcaaca ataaggtatg agttgtaaga aattccgttg ggaacaagat
     1801 actgactttc aaaaaggtcg attgtcttgt catcaacgcc aatgtatttt atagaatcgg
     1861 aaattttaac ctgcattgta ttctctcctt tataaatctg tgtccgtatt tgtcggaaat
     1921 ttttgttaag tcaattatac tatgttatca agttgaaatc aagatgatta ggataattta
     1981 aacactatgc aaattttgtt catgtgtctg attgtatatg aaattatttt gctattgatt
     2041 taaaagaaaa gcgatgatat aatattgtta aagaaaaata caaacgggtt gttattatgg
     2101 aagtaaaata ttcaaaagct gcactcaagt tccttgcaaa atccgataaa gcaacagtta
     2161 atcgtatcag aattggaatt gaggggttaa aaattaatcc tcctaaaggt gatataaaat
     2221 taatgcaggg atttgatgac ggcagacaac gattaagaat cggtaaatac cgtattattt
     2281 ttaaaaggtt acttgacggt acttatgaaa taatatatat tatggatata ggttctcgtg
     2341 gagatattta taaatgaaag gagttgccgt atatgagtac gaaaacagct gaaattgcta
     2401 aaatgattga tatgcttccc gacagtgaca aggaacttgc ctacgaattg attaagaaac
     2461 tcgtacttgc ttgggatcct gactacacaa aagttaccga acaggagtat aaagatatta
     2521 cagaagcgga aaacggtgac tttgtttccg aagacgatat tgattgggat aatctttcaa
     2581 gatatgcaaa ctaaataatt gcattaattt agcggtggca ttgaccaccg ctttttattt
     2641 tagtttttaa tattttgagt gtatgacatt tacccaccaa agcataattt tccactttca
     2701 attttcaatt aacaaaacag tccctttcaa tgcatttgca aattgaaagg gactgattgt
     2761 ataaatatta aaaagtatta gatttcagat tacaaaaatt actttctgtt gtttctgtta
     2821 aagccgcctc tgcggtcatt gtgaggtctg cgtggacgag ctgctctttc tgcgtcaatg
     2881 tcgttttcgg gaacaagacc gtcaacatca ataagaacct gacggcgaga gaggttaagt
     2941 ctgcccttat cgtcaatttc cattacctta acacggatga tgtcgcctac attaacaaca
     3001 tctgttacat tctctgtacg cttaacatca agctgagaaa tgtgaacaag accttcctta
     3061 cccggagcga tttcaacgaa agcaccgaag tccataatac gggtaacctt accgaggtac
     3121 attgcacccg gctcaggatc aacagcgatt gtgttgatga tttcgattgc acgcttacac
     3181 ttgtcggaat caatacccga aacaaatacc tgtccgagtt caccgtcttc ctcaatgtca
     3241 accttaactt cgcattcagc ctgaatttcc ttgataacct taccgctctt accgataacc
     3301 tcgctgattt tgtcagcagg aattgttgtt gtgaacatct tgggagcgta ctttgaaagc
     3361 tcttctctcg gctctgcaat agccttgagc attacttcat caagaatgta gttacgagcc
     3421 ttgtgagtct tggcaagagc ctctttaacc atttcgggag taagaccgct gattttgagg
     3481 tccatctgaa tagctgtgat accgtcatgt gtacctgcta ccttaaagtc catatcaccg
     3541 aagaagtcct caagaccctg aatatcaacc attgtcatcc aacggtcgcc ctctgtgata
     3601 agaccgcatg agatacctgc aacaggagcc ttaatcggaa cacctgcgtc cataagagca
     3661 agagttgaac cgcagattga accctgtgaa gttgaaccgt ttgaagaaag aacttccgaa
     3721 acaaggcgaa gtgcatacgg gaattcttct accgaaggaa ttacaggaac gagagctctt
     3781 tctgcaagag caccgtgacc gatttctctt ctgcccggac cacggcttgg ctttgtttcg
     3841 ccgactgagt agcttgggaa gttgtagtgg tggatatatc tcttttctgt ttcaccgtca
     3901 atgccgtcaa gaagctgttt gtcggaaaca ggaccgagtg ttgcgattgt gagaacctga
     3961 gtctgtcctc ttgtgaacat acccgaaccg tgaactctcg gaattacgcc aacctctgat
     4021 gcgagaggac ggatatcgtc cattcctctg ccgtcaacac gcttctgctc atcgagtaac
     4081 caacggcgaa caatgaactt ctgtgtctta taaaggcact cgtcaatgag agcttcgctt
     4141 tccggataaa tctcgtcaaa ttttgcatga actgcctcat agataggctt gagtctttcg
     4201 tcacgaattc tcttatcatc tgtatcaaga gcaaccttaa catcttcttc tgcaaatgcc
     4261 ttgattgcct caaacatatc atggtcgggt tcaagacttg caaactcaaa cttaggcttg
     4321 ccgatttcag ccttgatttc ctcaataaag ctgataatct tctggttagc ctcgtgacct
     4381 gccataatag cgttgtacat atcgtcatca gatacgcagt tagcacctgc ctcgatcata
     4441 gcaattcttg tgcttgttga ggcaactgtt gttgccatct gagaaacctt tctctgctcc
     4501 tctgtcgggt tgatgatgta ctcaccgtca atcataccta cagaacaacc cgagataggt
     4561 ccgttccacg gaacatcaga aattgaaatt gcgattgaag caccgatcat ggcaacaatt
     4621 tcaggctgac agtcgggatc aacgctcatt accgtacaaa cgattgaaac atcgtttctc
     4681 atgctcttgt caaaaagagg acggatagga cggtcgatta cacggcttgt gaggatagcc
     4741 ttttccgaag gtctgccctc tctcttgagg taagaaccgg ggattttgcc tactgcgtac
     4801 atcttttctt catagtcaac cgaaagcggg aagaaatcaa cgccctctct tggcttttct
     4861 gatgctgtaa cagcaacatg aactgttgtg tcgccgtagt gaacaagaca cgcaccgttt
     4921 gcaagctgtg tcatcttgcc tgtttcaacc tttaaaggtc tgccggcaaa ttctgtttca
     4981 aatactctgt atttgtcgaa tgtcataagt cgattcattt tagtaaaatc ctttccgaaa
     5041 cataagtttc acattttaaa taaagttcaa atttattcaa acgggatttt acgccgattt
     5101 agcaataaaa ccattaaaca aaactgacaa acacacaaaa gcatatgtta tttaatcgtt
     5161 ttaccgctaa aaaacagcaa ataaaaatcc cgcagaaaat cgaatttagg gcaagctttc
     5221 gcttgccctt gtgtacttaa aaaattactt acggataccg agtcttgcaa tgatagaacg
     5281 atatctttca atgtctacct ttgtgaggta agcgagaagg cttcttctct gaccaaccat
     5341 cttgagaaga cctcttctgc tgtggtgatc cttcttgtga accttgaggt gctcggtaag
     5401 gtcattgatt ctctttgtaa gaagagcaat ctgaacctcc ggagaacctg tgtcgccttc
     5461 gtgagttgcg tactcagcca tgatagcctg cttttcttct ttaagcatta aaaattccac
     5521 ctttcaaaaa tagtcgccaa catctcaggg cagggctgtg aaaatccgac aaacggcata
     5581 ctccctgttc cgagacacgg tcatctgttt tatattatca caatcaaagt caaaagtaaa
     5641 gtgtttttta caaaatcttc tttgcttttt cattgtagag catatctata tattcacaaa
     5701 ttttcttctg atcttcttta gacattgagc gatatcttgc aatgattgct ctttcggaaa
     5761 gagtcagtac attgagcgtc atgtcaccgt cggcaactct tctttcaaaa taatctctgt
     5821 tgtcaagaat tgcgttataa tcgataccga acattttaac gatttttccg agaagctcgt
     5881 ggcttggttc ggaaactccc gtttcatatt ttgtataagt tgttctgttt atctttaatg
     5941 cgtccgcaac ctgctgttgg gtaagtccgc actgaattct gtaataacgc attctgagtc
     6001 ctaccgaagc gtcgatatta ctgcactttc ttttgggcat cttctgcaca tcctatcaca
     6061 taaaagtatt tccgctttgc acactgcgat tcaattataa tacttttaaa taaaatattc
     6121 cacaaatgtg ataacacttc actaaacaaa aatcggcatg atttttgtgc attattttgt
     6181 attcataaat atttttttga tgaattatac ctgcatctat tattattgat tcgtaaaact
     6241 tttatgcctc tcttccggtt tttgtaaacc tctttttcaa atttggtatt gaaatcttta
     6301 aatttttgat gtataatcat gtagcggaag ttagtgtgta tgtttttatg caatttctgc
     6361 gttctgtatt tttgctttat tacagccgaa caaccgaaaa attaaacgct ggagtagcgc
     6421 agttggtagc gcaactgatt cgtaatcagt aggtcgaggg ttcaagtccc ttcttcagct
     6481 ccataacaaa aaccgcatca gaaagccgtt ttcaggctct ttggtgcggt ttttatttgt
     6541 ttatttttac cgtaaaatat tgcttaaata ttagtcaaat gtcagtcaaa aagcagcaat
     6601 cacatcaatc acacatatat acctaatgca tataaaacta aaagcaccct gcattttgca
     6661 aagtgcttta aaaattactc tttcgtattc gttttagccg gatttttaat cattctgccg
     6721 ttgacgaaga tgtagtcatc atcggagttt gcgttttcga ctgcttcggt ttcacgctga
     6781 acttcttctt ccgtaaggac atactgcaca ttatcacctg taaagtaacc gacagactcc
     6841 tgcacgcctt ttgcgattac ggatactatt atataggttg caatcatcgg gtagaaaatt
     6901 accgagagag caactgcaac aacaagcatt gcaccctttg catagattac tgcgaggaat
     6961 actgcgaatg aaactattac gacaagcaca agtgcgacaa tatttctgag aattttaccg
     7021 aaaatcaaca ggagtgaatt tttataaatg tcacggagtc tgaggtcata ggtaattgtt
     7081 agcagaggta catagaacag cataatcagc attgcaattg agaacactat atatatggta
     7141 aagacagagc cgtaaacgat gtctgtctga gcaagtgttc cgtaatagag aattgcaaaa
     7201 aacgaacatg ctactatgag gtaagcgaca aatccgttga aaattgattt tttcagattt
     7261 tcacgaaatg cctgtacaaa agttttgaaa acatttacat cctctttttc aaccgaaatt
     7321 ttgcggatta ccattacaag tccggaatag aacggcattg cgggaattat acccaagctc
     7381 cataatatta cattgttgaa tccggacaga aagcttatga gaatgaatat tccggaaaat
     7441 acggcaaacg gaattgagta catcaatccc gcaaataaca gcttaggaaa tcgggtgaag
     7501 aatcgtgcaa gtgatgattg attgaaattt ttcataattg ctaaattaac cttcttaaaa
     7561 aacaaatttg acataatttt agcacaaatc gcttgtcatt ttcaatagaa tgaccatatt
     7621 tgacaggtta caatattgta actttaatgt tactttaata tttcttattt gacaaataag
     7681 tcaaaaaact cagataatat gcggttgttt tttgtttgat tttgaaatta catttggtca
     7741 ataccttcgt tttgactaat cacagagaaa ttttttaaaa aaatctattg tattttcata
     7801 aatagtgtgt tatgattgtg gcaatagaga gaacacagaa tgttgttctc tcgccaatat
     7861 ccgggtttag cggataaata tttttaatta aggtgaaagc tatgaaaaaa tcaacaatca
     7921 aaagagctgc agctcttgca gttgcaggta tgatgactgc tactgctctt gcaggctgcg
     7981 gcggttcttc atcatcaggt tcatcaaaag atgattcaag tgcagcaggc aacgcaaaaa
     8041 acggtaaagt ttactatctt aactttaagc ctgagcagga caaggattgg caggctcttg
     8101 ctaagaagta cacggacgaa acaggtgttg aggtaactgt taagacagct gccgagggta
     8161 cttatgagtc aacacttaca gcagagatgg acaaggataa cgctcctaca ctcttccagg
     8221 ttaacggtcc tgtcggtctt gctaactgga aagactattg tgcagacctc agctcatcag
     8281 aaatttataa tcagcttact tcaaaggatt atgcacttga agaggacggc aaggtttacg
     8341 gtattgctta tgtaattgaa acatacggca ttatctacaa caagactctt ctccagaagt
     8401 attgcgattc tgactttgct tcggttaaga agattgaaga catcaacagc tttgagaagt
     8461 taaagactgt agccgatgaa atccagaaga ataaggataa gctcggcgtt aagggtgcgt
     8521 tcacatcagc aggtatggat tcttcatctg actggagatt caaaactcac cttgctaacc
     8581 ttcctatcta ctttgagtac aaggataagg gcatcaaatc aacagacgct atcgagggta
     8641 aataccttga aaactataag aacatctggg atctctacat cacagattca acatgtgatc
     8701 ctgcagacct cgcaagcaag aagggtacag acgctgaaac tgagttcaca tcaggcgaag
     8761 cagtattctt ccagaacggc agctgggaat attcaacagt taccaaggca ggtatgaagg
     8821 acgacgaact cggcatgctc ccgatttaca tcggcgcagg cgatgaagct aatcagggtc
     8881 tttgcacagg ttcagagaac tactggtgtg taaacaacaa ggcaagtgct gacgatcagg
     8941 cagctactct tgaattcttg aactggtgtg taacaaacaa aacaggtaca tctgctcttg
     9001 ctgacgatat gggatttgta attcctttca agaatgctaa ggaagcaacc aatgtatttg
     9061 ttaagattga taacgagctc acagcagcag gcaaaactcc ggtaagctgg aacttcacaa
     9121 caatgccaag tgaagattgg aaaaacggtg ttggcacagc tcttacacag tatgcagcag
     9181 gcacaggcga ctgggatgct gttaagactg catttgtaca gggttggaaa acagaatatg
     9241 caaaggctaa cggctgattg taaaatttct gttttaattt aacaggttcg gtaggggggc
     9301 ggcattccgc ccccctttct tatgaacgga aaggcaaaat attatgttta gaaaacctaa
     9361 aagaaaaatt tattttctta tatttgttct tcctactctt gctgctttcc ttataggttt
     9421 tgtatatccg ttctttaacg gcatttacct ttcattctgt aaattcagaa caacaagcga
     9481 cgcagtattc attgggttcg gaaactatgt caaggccttt cagaatgagg gctttttaca
     9541 ttcattctgg tttacggtac tgtttgttat agtatcggtt atactcatta acctcattgc
     9601 gttttttgtg gcgtatgcac tcacgctcgg aatcaggggt tcaaatgtat tcagaactgt
     9661 tttctttgtt cctaaccttg taggcggtat cattctcggc tacatctggc agtacatttt
     9721 taacggtatt cttcagtatt acggaacaaa ccttgcgctt aacgcaaact tcggcttctg
     9781 gggacttgta attcttatgt gctggcaaca ggcaggttat atgatgatta tttacattgc
     9841 cggtttccag agcgtacctc atgatcttta tgaggcggct aaaattgacg gcgcaggcaa
     9901 aaaacagttg cttacaaatg taacattgcc gatgatgatg ccttccatca caatctgcac
     9961 attccttaca cttacaaact catttaagct gttcgaccag aaccttgccc tcacaggcgg
    10021 tgctccgggt attgtatcac agggaagcgg tacaatggtt tatcagacag aaatgatggc
    10081 tctcaatatt tacaatacat tctatgcaaa ccagaattct cgtggcgtag gtcaggctga
    10141 gggcgttatc ttcttcgtaa ttgttgttat cattgccctt attcagcttt acttcacaag
    10201 aagaaaggag gttcagcagt aatggcaaaa gttgcagcaa aggctaagga taggcctgta
    10261 aagaaaaaac aaaccgtagg aatgaaaatt ttaacaattc tcttctctat tgtgtcagta
    10321 ttctatgttg ctcccgtttt cattgtactg atgaactcct tcaagagcaa tgcggcaatc
    10381 agccgtgcaa tctttgaatt tccgaatgca aacagttttc agggctttca aagctatatg
    10441 acaggtatgt tccacggcaa ctatccgttc tggaagtcaa ttgtatggag cttcttcatt
    10501 acaatcggcg gtgttgtgct tattcttttc tgcacatcaa tgtgtgcgtg gtacttgcag
    10561 cgttcaaatt ctcgtatatg caaaatcatc tactatttct gcattttctc aatggttatt
    10621 cctttccaga tggtaatgtt taccctctca aagacagccg acagtctcaa gcttacaact
    10681 ccgtggggta ttttgattat atacctcgga ttcggagcag gacttgcggt atttatgttc
    10741 tcgggatttg ttaaatcaat tcctgttgaa attgaggaag ccgcagatgt tgacggagca
    10801 ggtcctttga gaacattctt cggtattgtt cttccgatta tgaaacctac ttttatttcg
    10861 gttgcaatcc ttgaaacaat gtggatttgg aacgactacc ttctccccta tcttattctt
    10921 gatatggatt acaaaactat tccgattcat attcagtatc ttaacggcag ttacggtcat
    10981 acggacacaa gtgctattat ggcgcttatc gttctgagca tcgttccgat tattattttc
    11041 tactttgcat gtcagaaata catcatcgag ggtgttgttt caggtgctgt aaaaggttaa
    11101 tctttctttc attttatttt caaaaaacgg ctgtgtacat tatgctacac agccgttttt
    11161 ttctcttgtg ttttttctct cgtgtttttt ctctcgtgtt tttttctctc gtgtagacag
    11221 tttgtataat gcaaaacaaa ttatgcccga gcgtaacatt gcaattgtct gtgtaatcca
    11281 accgtatgag cgttctttgg actcccgaaa gtaatgatgc aatatgtttt ttgatattac
    11341 aaactatcta cacgaacagg gtacggtggc tcgccgtgtg ggcggataat atccgcctct
    11401 acatttaaac
//
LOCUS       DAOMVE010000052        11371 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1348_length_11371_cov_3.479410, whole genome shotgun sequence.
ACCESSION   DAOMVE010000052 DAOMVE010000000
VERSION     DAOMVE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11371
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1348_length_11371_cov_3.479410"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..>250)
                     /locus_tag="PLS64_04355"
     CDS             complement(39..>250)
                     /locus_tag="PLS64_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43096.1"
                     /translation="LANCLRCVTLTLINALNFKVYRLKYSRNALHINVFPFAPYFRQR
                     FAPSFRHWRRSQGYTFEPLVWQKNL"
     assembly_gap    251..260
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            675..750
                     /locus_tag="PLS64_04360"
     tRNA            675..750
                     /locus_tag="PLS64_04360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:708..710,aa:Val,seq:tac)
     gene            756..832
                     /locus_tag="PLS64_04365"
     tRNA            756..832
                     /locus_tag="PLS64_04365"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:790..792,aa:Asp,seq:gtc)
     gene            839..914
                     /locus_tag="PLS64_04370"
     tRNA            839..914
                     /locus_tag="PLS64_04370"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Phe,seq:gaa)
     gene            922..997
                     /locus_tag="PLS64_04375"
     tRNA            922..997
                     /locus_tag="PLS64_04375"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gly,seq:gcc)
     gene            complement(1095..1757)
                     /locus_tag="PLS64_04380"
     CDS             complement(1095..1757)
                     /locus_tag="PLS64_04380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRL43097.1"
                     /translation="MAVMNVNPTRMQLTKLRKQLATATRGHKMLKDKRDELMRRFLDL
                     VRENKELREKIERELAECNNHFVNASAVMSKEALDASLMSPKQRIDLELSSKNVMSVD
                     IPQFSSSTRTSNESDIFPYGFAFTSFELDDAVMSLNELLPDLIRLAEIEKSCELMSAE
                     IEKTRRRVNSLEHVMIPRYQETIKYISMKLEENDRSSRTRLMKVKDMLLDKAHHYSEH
                     YN"
     gene            complement(1779..3152)
                     /locus_tag="PLS64_04385"
     CDS             complement(1779..3152)
                     /locus_tag="PLS64_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRL43098.1"
                     /translation="MPKEYRTIREVAGPLMMVSDVEGVTYDELGEIELPNGETRRCKV
                     LEVDGSNALVQLFESAAGINLANSKVRFLGRSMELPVSPDMLSRVFDGLGNPIDGGPA
                     LIPEKRLDINGTPMNPAARNYPQEFIQTGISAIDGLNTLVRGQKLPIFSASGLPHAQL
                     AAQIARQAAVRGKDEQFAVVFAAMGITFEESDYFVQSFKETGAIDRTVMFVNLANDPA
                     IERIATPKMALTAAEYLAFDRGMHVLVIMTDITNYADALREVSAARKEVPGRRGYPGY
                     MYTDLATLYERAGRLKGKEGSITLIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     RKGITPPIDVLPSLSRLKDKGIGPDRTRKDHAATMNQLFAAYARGKDARELMIILGEA
                     ALTDMDKKYAEFAEAFEREYVSQGYDANRSIEETLDIGWKLLHILPRSELKRIKDDML
                     DEFYGKE"
     gene            complement(3152..4918)
                     /locus_tag="PLS64_04390"
     CDS             complement(3152..4918)
                     /locus_tag="PLS64_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRL43099.1"
                     /translation="MKSGIITKVAGPLVIAEGMRDADMFDVVRVSSQRLIGEIIEMHG
                     DRASIQVYEETSGLGPGEIVESTGAPLSVELGPGLIGSIYDGIQRPLNEIMKVAGTNL
                     KRGVDVPSLDHKKKWHFTPLVKKGDTVAAGDTLGTVQETHAVLHRILVPNKTGGVVES
                     ISEGDFTIDETVAVLKTDKGNVEINMCTKWPVRVGRPYKRKLSPDILLTTGQRPIDTL
                     FPLAKGGVAAVPGPFGSGKTVVQHQLAKWAAADIIVYIGCGERGNEMTDVLNEFPELK
                     DPRTGNSLMERTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYTVALMADSTSRWA
                     EALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGRVIVNGSDDTEGALSVIGAVSPP
                     GGDISEPVSQATLRIVKVFWGLDANLAYKRHFPAINWLTSYSLYTDRLADWFSKNAAP
                     DFMELRGKLMTLLQEESELQEIVNLVGMDALSAPDRLKLETSRSIREDYLHQNAFDPT
                     DTYTSLGKQVLMMRAILAYYDKAKEALANGADIEMLVNLPVRERIGRYKYVPEDKVRD
                     EFESIKALLDSEIKDVLERSED"
     gene            complement(4930..5253)
                     /locus_tag="PLS64_04395"
     CDS             complement(4930..5253)
                     /locus_tag="PLS64_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRL43100.1"
                     /translation="MAKNIAVIGDSESIKGFAAIGMDIYPCDDNENAPHLFRKIADGD
                     NYAVIFITEEMFGLVEKERKRYEERLIPAVIPIPGVKGNTGIGIKRLSSFVEKAVGSD
                     IIFND"
     gene            complement(5246..6217)
                     /locus_tag="PLS64_04400"
     CDS             complement(5246..6217)
                     /locus_tag="PLS64_04400"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRL43101.1"
                     /translation="MALSYEFSIGSVRAKEKNLFTNSDIEHMLGCENVNELCRYLSDK
                     GYGEGDDIEEILKSHSENVWEYLKRTAPDFAIFKPFFYLNDLHNLKAVLKGTLSNRPY
                     SQLLVKPCTFSEETLKQAVENRKFSLLGEELSAPCDKAYEILAHTGDARLSDAVLDRA
                     FMELVLKTSEKSDSEFMSEYFKATVFYNNVKTAIRGAKADCDRDFLEIAICDVQDFPR
                     SRVIEASVKGLEPTLDVLSKISAYGCNKAVEEYKASPSAFEKFVDNRLMDLAKEKCKR
                     SGDGADPITGYLIASETEKKVIHIIASGIRTKTNYETVKERLREVYG"
     gene            complement(6229..6810)
                     /locus_tag="PLS64_04405"
     CDS             complement(6229..6810)
                     /locus_tag="PLS64_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRL43102.1"
                     /translation="MSSGDKILNRISLDCDERISQIGAETDEKCAQIMAQAKLDADKI
                     SAEIADRAQSKVKQMQAASKSRCDLETRNAFLKRRREEIDKTYSEILNKMKNLPDEDY
                     FELIYTFAKKLNGMSGVVLLNEKDMNRLPKDFLARLEECGVKAELSKTPCDIESGFIL
                     KCGDIEENMDFSAILSEKRDAIEDFINQELFKA"
     gene            complement(6852..7325)
                     /locus_tag="PLS64_04410"
     CDS             complement(6852..7325)
                     /locus_tag="PLS64_04410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HRL43103.1"
                     /translation="MNGIFFALLGASIATALAGVGSARGVGSAAQAAMGVLSEDSSKF
                     GKMLVLTLLPGTQGLYGFIVGFLILVSGGVLGGTAPTIGQGLAYFAASLAIGIGGMIS
                     GFAQGKAAVSGIALSAKDDSNFSKAMVSVTLVEIYALLSFIVSLLVVITVPNLNI"
     gene            complement(7322..9310)
                     /locus_tag="PLS64_04415"
     CDS             complement(7322..9310)
                     /locus_tag="PLS64_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HRL43104.1"
                     /translation="MAKLKMKSVRIIALRQDRKRLLEHLQDSGLVQIEKTETCRKGFG
                     KIDMTSQIQIFERNVTLSENALKILDSNSPEKKSMLSAFCGRREIDPDEIGVIASNAG
                     EVIDVCNRICELNKQIADNAAERIRIKTALAQLEPWKNLDIPLNAKDTKTTAVFIGSI
                     QKEYNEVSLSKALAEASPKLEFDFEIQFANEVLTCIVLFAPIAQKELAENALRDIGFA
                     KPVTNSSLTPLAESKKLVERSHKLEDDTENAKKEIISFADRRKDIKDTQDYFRIRADK
                     YSVIGELQHSRNVFVISGYIPEEDCEKLERLCERVSVCYVEFGDVDEEKAPVKLKNSR
                     FAAPAESIVNMYSPPSHDDIDPTPLLAFFFYFFFGMMFSDAGYGLLMVIGTGLAIKLF
                     KPDKEMRNTLKLFQYCGVSTFFWGLVFASFFGDAPATLYNYFTGADITMKQIFPWPTI
                     DPQKDALMLMIISIAFGLVHILVGMGCKFYVCLRQKDYGGAFFDTGLWMLMLIGFAVL
                     AAGMAFGQTLVYVGAGIAIFCAIGLVLTQGRNKKGFGKVIGGLASLYDITGYISDLLS
                     YSRLLALGLTTGVMAQVFNMLSTMFGKSWFGIILLIIVFIIGHAINIGLNALGSYVHT
                     MRLQYVEMFGKFYEGGGKQFKPFKLNSKYIKIQEDKSK"
     gene            complement(9345..9656)
                     /locus_tag="PLS64_04420"
     CDS             complement(9345..9656)
                     /locus_tag="PLS64_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43105.1"
                     /translation="MAKDMLDAIYNAEEDCRQREANARAESAERVEQTKADAKQVVLS
                     AKEKAQKDADMLFEKTAKEGKKELEKASEKANLECDILSRTADKNRKRVIDGAIQRLS
                     L"
     gene            10254..10733
                     /gene="ybaK"
                     /locus_tag="PLS64_04425"
     CDS             10254..10733
                     /gene="ybaK"
                     /locus_tag="PLS64_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HRL43106.1"
                     /translation="MKEIKTNVMRILDKEKVEYTHHEYPHGKDAVDGVTVATLMNQNP
                     DYVFKTLVTKGMGRDYYVFVVPVDHELDLKKCAKSVGEKSVEMIPVKDITKVTGYVRG
                     GCSPLGMKKQFKTTFHITAESIPKIIVSAGKIGYQIDLKPEDLIRLTNGQCADIIKD"
     gene            complement(10806..>11371)
                     /locus_tag="PLS64_04430"
     CDS             complement(10806..>11371)
                     /locus_tag="PLS64_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL43107.1"
                     /translation="AGTDKMTMEAVSQLMLMLLLGIILIYLIMVAQFQSLKSPFIIMF
                     TIPLAFTGGFLALLITGFDISVVSLVGFVMLCGIIVNNGIVLVDYINRLRIDGKERIE
                     SIVEAGKTRMRPILITALTTVLGLVVMALGIGTGAEMMQPIAIVCIGGLLYATFMTLY
                     IVPVIYDLFNKKELKKVSDEDLEFIDE"
BASE COUNT         3047 a         2579 c         2417 g         3318 t
ORIGIN      
        1 cattaaatca gtacagttga tgtactgatt tttttatttt acagattttt ttgccaaaca
       61 aggggctcga aggtgtagcc ttgcgaccgc cgccagtggc ggaaagaggg agcaaagcgc
      121 tgtctgaaat aaggagcaaa aggaaataca tttatgtgca acgcattgcg actgtatttc
      181 agacggtaaa ccttaaaatt aagagcgttg atgagggtta gggttacgca ccgcaagcag
      241 ttggcgaggt nnnnnnnnnn ctccaactaa gacagtacag aaattgtgct gtcttttctt
      301 tttgtacaat ctcgtagggg cggatattat ccgcctgcaa gtaactactg aattcttttg
      361 acagacccga ttttataaat gtcgcaaaac aattgttata ctgtaacagc gaactgcgct
      421 ttcaaataat accgtgtgaa aacacggggc gaaactcttg tttactgttt gaaataagga
      481 gtttaccata cttgtattaa taacttcagc gaatatgttc tgattgccga taatcacgaa
      541 atcgcaatta gcaaataaat tttaaaaaac tgcaaaaacg gtattgacaa tcgactgatt
      601 ttatagtatt atattatctg tcgctgagat gtaatcgaaa ttcgacagaa cttcgatgag
      661 cgatataggc atgtgggagc atagctcagc tgggagagca tctgccttac aagcagaggg
      721 tcacaggttc gagccctgtt gttcccacca catttggccc ggtagttcag ttggttagaa
      781 cgctagcctg tcacgctaga ggtcgacggt tcgaacccgt tccgggtcgc caatttttgc
      841 ctctgtagct cagtcggtag agcaggggac tgaaaatccc cgtgtcggtg gttcgattcc
      901 gcccggaggc accaataaaa tgcggatgta gctcatctgg tagagcgcca ccttgccaag
      961 gtggaggtag cgggttcgag ccccgtcatc cgctccatta aatcagtaca gttgatgtac
     1021 tgattttttt attttacaga attttttgac aaacaagggg ctgtttttta acagcccctt
     1081 tgattgttgt gtagttaatt atagtgttcc gaataatgat gtgccttgtc aagaagcata
     1141 tccttaacct tcatcagcct tgtacgactg ctgcggtcat tttcttcaag tttcatcgaa
     1201 atatacttga ttgtttcctg atatctcgga atcataacat gctcaagcga attaacacgg
     1261 cggcgtgtct tttcaatttc tgccgacata agctcacagc ttttttcaat ttcagcaagc
     1321 cttatcaggt cgggcaaaag ctcgttgagc gacataaccg catcatcaag ttcaaacgaa
     1381 gtaaacgcaa aaccgtaggg aaaaatatcg ctctcgttgc ttgttcgtgt gcttgatgaa
     1441 aactgcggaa tatcaacgct cataacattt tttgaagaaa gctcaaggtc aattctctgc
     1501 ttcggtgaca tcaatgaggc gtccagagcc tcttttgaca taaccgcact tgcgttcaca
     1561 aagtggttgt tgcactctgc aagctctctt tcaatcttct cacgaagctc cttgttttct
     1621 cttacaaggt caagaaatcg gcgcatcagt tcgtcacgct tgtcttttaa cattttatga
     1681 ccacgggtag ctgttgcaag ctgttttctc agctttgtca actgcatacg ggtgggatta
     1741 acattcataa ctgccattag caaacacctt caatgcgatt attctttacc gtaaaactcg
     1801 tcaagcatat cgtccttaat tcgtttaagt tcacttctgg gaagaatatg caaaagcttc
     1861 cagccgatgt caagtgtttc ttcaattgaa cggtttgcgt cataaccctg tgaaacatac
     1921 tcacgctcaa atgcctcggc aaattcagcg tactttttat ccatatcggt aagagccgcc
     1981 tcaccgagaa ttatcataag ttctcttgcg tctttacctc ttgcataagc ggcaaaaagc
     2041 tggttcatgg ttgcggcgtg gtctttacgg gttctgtcgg gaccgatacc cttgtcttta
     2101 agtcttgaaa gtgacggaag aacatcaatc ggcggagtaa ttccctttct gtaaagctca
     2161 cgggatagga taatctgacc ttctgtaatg tagcctgtaa ggtcaggaat cgggtgagtc
     2221 ttgtcatctt cgggcattgt aagaatcgga ataagagtga ttgaaccctc tttgccttta
     2281 agtctgccgg ctctttcata caatgttgca aggtctgtgt acatataacc cggatagcct
     2341 cgtctgcccg gaacttcttt tcttgcggca gaaacctcac ggagcgcatc ggcatagttt
     2401 gtgatgtcgg tcataataac gagaacgtgc attcctctgt caaatgcaag atattcagcc
     2461 gccgtgagtg ccattttagg agttgcaata cgctcaatgg caggatcgtt tgcaaggttt
     2521 acaaacatta cggttctgtc aatagcgcct gtttctttaa acgactgaac aaagtagtcg
     2581 gattcttcaa atgtaatacc cattgcggca aatacaacgg caaactgctc gtccttgcct
     2641 cttacagccg cctgtcttgc aatctgagcg gcaagctggg catggggcag accagatgcc
     2701 gagaagatag gcagcttctg tcctcttaca agtgtgttaa gtccgtcaat tgctgaaata
     2761 cctgtctgaa taaattcctg cggatagttt cttgcggcag ggttcatagg tgttccgttg
     2821 atgtcaagtc ttttttcggg aataagagca ggaccgccgt caatcggatt tccaagaccg
     2881 tcaaatactc gtgaaagcat atcgggagat acgggaagtt ccattgaacg acctaaaaat
     2941 cttacctttg aatttgcaag gttaatgcct gcggctgatt caaaaagctg aacaagtgcg
     3001 tttgagccgt caacctcaag aaccttgcaa cggcgtgttt caccgttcgg aagttcaatt
     3061 tcgccgagtt cgtcatatgt tacgccctca acatcactta ccatcataag aggacctgca
     3121 acctctcgta ttgtgcggta ttcctttggc atcagtcctc actcctttca agtacatcct
     3181 taatttccga atcaagcaga gccttgattg actcgaactc gtcccgaacc ttatcctcgg
     3241 gtacatattt atatcttcct atgcgttcac gaacaggaag gttgacaagc atttcaatgt
     3301 ctgcaccgtt tgcaagagcc tcttttgctt tgtcatagta agcaagaatt gcacgcatca
     3361 taagcacctg cttgccgaga gatgtgtatg tgtctgtcgg gtcaaatgcg ttttggtgca
     3421 gataatcctc acggattgaa cgggaggttt caagcttaag tctgtcgggt gcggaaagtg
     3481 cgtccatacc gacaaggttt acaatttcct gaagctcgct ttcttcctgt aataatgtca
     3541 taagtttgcc acgaagctcc ataaagtcgg gagcggcatt ctttgagaac caatctgcaa
     3601 gtcggtctgt ataaagcgaa taacttgtca gccagttaat tgcagggaag tgtcgtttgt
     3661 aagcaaggtt tgcgtcaagt ccccagaata ccttaacgat tctgagtgtt gcctgtgata
     3721 caggctctga aatatcaccg cccggaggtg acacagcacc gataaccgag agtgcgcctt
     3781 ccgtgtcatc actgccgttt acgataacac ggcctgctct ttcatagaac tgtgcaagtc
     3841 ggctgccgag gtatgcggga taaccctctt cacccggcat ttcttcaaga cggccggaca
     3901 tttcacggag agcctctgcc cagcgggatg ttgagtcagc cataagtgca accgtgtaac
     3961 ccatatcacg gaaatactcc gcaatagtaa tgcctgtgta gatagatgcc tcacgggcgg
     4021 caacaggcat atcggatgtg tttgcaataa gcacggttct ctccataaga gagttgcctg
     4081 tgcgtgggtc tttaagttcg gggaactcgt taagaacatc ggtcatctcg ttgccacgct
     4141 caccgcaacc gatatatacg ataatatcgg cagccgccca ctttgcaagc tgatgctgaa
     4201 caacagtttt gcccgaaccg aacggtcccg gaactgcggc tacaccgccc tttgcaagcg
     4261 gaaagagtgt gtcgataggt ctttgacctg tggtaagaag aatgtcgggg gagagctttc
     4321 ttttgtacgg tctgcccaca cgaacaggcc attttgtaca catattaatc tcaacattgc
     4381 ccttgtctgt tttgagaacg gcaactgttt cgtcaattgt aaaatcgccc tcgctgatgc
     4441 tctcaacaac accgcctgtt ttgttaggaa caagaatcct gtggagaaca gcgtgtgttt
     4501 cctgaacagt accgagtgtg tcgcctgccg caactgtgtc gcccttttta acaaggggag
     4561 taaagtgcca cttctttttg tgatcaagtg acggaacatc aacacctctt ttgaggtttg
     4621 tgccggcaac cttcataatc tcgttaagcg gtcgctgaat accgtcataa attgaaccga
     4681 taagtcccgg accgagttca acggaaagcg gagcgcctgt ggattcaaca atctcaccgg
     4741 gtccgagtcc cgaagtttct tcatatacct gaattgatgc cctgtcaccg tgcatctcga
     4801 ttatttcacc gataagtcgc tgactgctta cacgcacaac atcgaacata tcggcatcac
     4861 gcataccctc ggcaattacc agaggacctg ctaccttagt tataattcca gatttcaaat
     4921 tcttacacct caatcattga agataatgtc cgaaccgact gccttttcaa cgaatgacga
     4981 cagtcttttt atgccgattc ccgtattgcc ttttacaccg gggataggaa taacggcagg
     5041 aatgagcctt tcttcatatc tttttcgctc tttttcaaca agtccgaaca tttcctcggt
     5101 aataaaaatt accgcatagt tgtcaccgtc agcaatcttt ctgaaaagat gcggtgcgtt
     5161 ttcgttgtca tcacacggat aaatgtccat gccgattgcc gcaaagccct ttatgctttc
     5221 gctgtcgccg ataacggcaa tatttttagc cataaacttc acgcaacctt tcttttacgg
     5281 tttcataatt tgtcttggtg cggattccgc ttgcaataat gtgaataact tttttctctg
     5341 tttcacttgc aatcagatag cccgtaatcg ggtctgcacc gtcgccgctt cttttgcatt
     5401 tttcttttgc aaggtccata agcctgttgt caacaaattt ttcaaatgcc gacggagaag
     5461 ccttgtactc ttcaacagcc ttgttacagc cgtatgccga gatttttgac agcacatcaa
     5521 gcgttggttc aagtcccttt acgcttgcct caataaccct tgaacggggg aagtcctgca
     5581 catcacagat tgcaatttca agaaaatccc tgtcacaatc ggcttttgca cctcttatgg
     5641 ctgtctttac attattataa aatactgtag ctttaaaata ttcggacata aattcgctgt
     5701 cggatttttc ggatgttttc agaacaagct ccataaaagc tctgtcaaga accgcatcgc
     5761 tgagtcttgc gtcacctgtg tgtgcaagaa tttcatatgc cttgtcacaa ggtgcggaaa
     5821 gttcttcgcc caaaagtgaa aactttctgt tctcaaccgc ctgtttaagc gtttcttcag
     5881 agaaagtaca cggctttacc aaaagctgtg aatacggtct gtttgaaagc gttcccttta
     5941 aaacagcttt aaggttgtga aggtcgttaa gatagaagaa cggtttgaat attgcaaaat
     6001 cgggtgcggt tcttttgaga tattcccaca cattttcgct gtgtgatttc aaaatctctt
     6061 caatgtcatc gccctcaccg taacccttgt ccgagaggta tctgcaaagc tcgttcacat
     6121 tttcacaacc gagcatatgc tcaatgtcgc tgtttgtaaa cagatttttt tctttagcac
     6181 ggacggagcc gatggaaaat tcataagata atgccatcat attcctccct atgccttaaa
     6241 aagttcctga tttataaaat cctcgatagc gtcacgcttt tcggacaata ttgccgagaa
     6301 gtccatgttt tcttcaatgt caccgcattt aagaataaat ccgctttcaa tgtcacacgg
     6361 cgtttttgaa agctctgctt ttacaccgca ttcttcaagc cttgcaagaa aatccttcgg
     6421 aagtcggttc atatcctttt cattcaaaag cacaacaccg ctcattccgt ttaacttttt
     6481 tgcaaatgtg taaatcagtt caaagtaatc ttcatcggga agatttttca ttttattaag
     6541 aatttcactg tatgtcttgt caatttcttc acgccttctc tttaggaaag cgttgcgtgt
     6601 ttcaaggtcg caacggctct ttgaagccgc ctgcatctgc ttgacctttg actgtgccct
     6661 gtcggcaatt tcggcagaaa tcttgtcggc atcaagcttt gcctgtgcca taatctgtgc
     6721 gcatttttca tcggtttcag cacctatttg gctgattctt tcgtcacagt caagacttat
     6781 gcgatttaaa attttatcac cgctgctcaa agtgattcca cctttctttc gtaattttcg
     6841 ttagtcggtt atcagatgtt aaggtttgga actgtgataa caacgagaag tgaaacgatg
     6901 aatgaaagga gagcgtaaat ctcaacgaga gttacggaaa ccatagcctt tgagaagttt
     6961 gaatcgtcct ttgcactgag tgcaataccc gaaacggctg ccttgccctg tgcaaaacct
     7021 gaaatcatac cgccgatacc gatagcaagc gatgctgcaa agtatgcaag tccctgtccg
     7081 attgtcggag ctgtgccgcc gagaacgccg ccgcttacga gaataaggaa gcctacgata
     7141 aatccgtaaa gtccctgagt acccgggaga agtgtgagaa cgagcatttt accgaatttt
     7201 gatgagtcct cggaaagaac tcccatagct gcctgtgcgg cagagcctac acctcgagca
     7261 gaacctacgc ctgcaagtgc tgttgctatt gatgcgccta aaagtgcgaa aaatattcca
     7321 ttcattttga tttgtcctcc tgaattttaa tatatttact gttgagcttg aacggcttaa
     7381 actgtttgcc gccgccctca tagaattttc cgaacatttc aacatactga agtcgcattg
     7441 tgtgaacata tgaaccgagt gcgttgagtc cgatgttgat tgcgtgtccg atgatgaaca
     7501 caataatgag taagataatt ccaaaccagc tctttccgaa catggttgaa agcatattga
     7561 acacctgagc cataactccc gttgtaagtc cgagagcaag aagtcttgag tagctcagaa
     7621 ggtcactgat gtaacctgtg atgtcataga gagaggcaag tccgccgatg actttgccga
     7681 atcccttttt gtttctgccc tgtgtgagaa caagaccgat tgcgcagaaa atcgcaatgc
     7741 ctgctccgac atagacgagt gtctgaccaa atgccatacc tgccgccaaa acggcaaatc
     7801 cgataagcat aagcatccaa agtcccgtgt caaagaacgc tccgccgtaa tccttctgcc
     7861 tgaggcaaac atagaatttg cagcccatac cgacaagaat gtgtacaaga ccgaatgcta
     7921 ttgaaataat catcagcata agtgcgtcct tttgcgggtc aattgtcggc cacgggaaga
     7981 tttgtttcat cgtaatgtct gcacccgtaa agtagttgta aagggttgcg ggggcatctc
     8041 cgaagaagct tgcaaaaaca agtccccaga aaaatgttga aacaccgcaa tactggaaca
     8101 gcttcaaggt gtttcgcatt tccttgtcag gcttgaacag ctttattgca agtcctgttc
     8161 ctattaccat taacagtccg tagcctgcgt ccgagaacat cattccaaag aaaaagtaga
     8221 agaagaacgc aagcaacggc gttggatcaa tatcgtcatg cgacggggga gagtacatat
     8281 ttacaatgct ctctgccggt gctgcaaagc gactgttctt tagctttaca ggagcttttt
     8341 cctcgtcaac atcgccgaat tcaacatagc atacagaaac tcgttcacaa agcctttcaa
     8401 gcttttcaca gtcctcctcg ggaatgtaac ccgaaataac aaaaacattc cgtgaatgct
     8461 gaagttcacc gattacgctg tatttatcgg ctctgattct gaaataatcc tgtgtatctt
     8521 taatatcttt tcgtctgtct gcaaaggaaa tgatttcttt ttttgcattt tccgtgtcgt
     8581 cttcaagctt gtgacttctt tcaacaagct tttttgattc cgccaaaggg gtaaggcttg
     8641 agttcgtaac aggttttgca aaaccgatat cacgcagagc attttccgca agctcctttt
     8701 gagcaatcgg tgcaaaaagt acaatacagg taagcacctc gtttgcaaat tgaatttcaa
     8761 aatcaaattc aagcttcgga gaagcctcgg caagtgcctt tgaaagcgat acctcgttgt
     8821 actccttttg aattgagcct atgaaaacag ccgttgtttt tgtgtccttt gcattaagcg
     8881 gaatgtcaag attcttccac ggctcaagct gtgcaagagc ggtttttatg cgaatccgtt
     8941 ccgccgcatt atcggcaatc tgtttgttaa gctcacaaat tctgttgcac acatcaataa
     9001 cttcgcctgc atttgatgcg ataactccga tttcatcggg atcaatctcc cgccgtccgc
     9061 aaaatgcgga aagcatactt ttcttttcgg gagagttgct gtcgagaatt ttcagagcgt
     9121 tttcactgag agtaacattc cgctcgaaaa tctgaatttg tgaggtcata tcaatcttgc
     9181 cgaaaccttt tctgcaggtt tcggtctttt caatctgaac caaacccgaa tcctgcaaat
     9241 gttcaagaag tcgctttctg tcctgtctta gggcaataat ccgaaccgac ttcattttaa
     9301 gttttgccaa tttctatcac cacgcttctt tcattgcaac cgttttaaag agagagcctt
     9361 tgtatagccc cgtcaataac acgcttgcgg tttttgtctg cagtccgaga gagaatgtcg
     9421 cactcaagat ttgccttttc agaggctttc tcaagctctt ttttgccctc tttcgcagtc
     9481 ttttcaaaaa gcatatcggc atccttttgt gccttttcct ttgccgaaag cacaacctgt
     9541 ttggcatctg cctttgtctg ttcaaccctt tctgctgatt ctgctcgtgc atttgcctca
     9601 cgctgtctgc aatcctcttc ggcattgtaa atggcgtcca gcatatcttt tgccattgtt
     9661 atcctccttt gttaaaaaat tgcataaaaa tatttttgaa gaatacacta ttttgtttat
     9721 tctattccta gattgtagca caattattta aaaaaagcaa tcaacataca ttaaagtttg
     9781 cccagttttt tatcattttc ttccctgtga ccgcatttgc gagtggtttt acagcattaa
     9841 tttgcgataa aagaaaaaac accgcataat acggtgttta aaaaattaac ttgcttatta
     9901 tcttacttac tgtcttgctt actgacttgc ttattatctt gcgtgttgtg ggtaaaacag
     9961 cgatatttta gtctgtaaaa cagaagaaag ccgtcccgaa aaaattcgga gcggctttca
    10021 aagctacggt tattcggcag gcgtcccatt ctcctgcatc tctcaaggtt tccctttgtc
    10081 aagccatcgg cgtgtggacg gggacgaaat cttccacctc gaataacttc tgaatttatt
    10141 ctaacattat tttaataaaa ttgcaaataa atcaaggttg aaatccgatg cgaaaagttg
    10201 tattatgtga gggtaataac aagatggcaa tatgcatgga ggattgtgta tttatgaaag
    10261 aaatcaagac aaatgtaatg agaattttag acaaagaaaa ggttgaatat actcaccatg
    10321 aatatcccca cggcaaggat gccgttgacg gagtaacggt tgcaacgctt atgaatcaaa
    10381 atcccgatta tgttttcaaa actcttgtaa caaagggcat gggcagagat tattatgttt
    10441 tcgtagttcc tgttgaccat gagcttgacc ttaaaaaatg tgcaaagagc gtaggtgaaa
    10501 agtcggttga aatgattccc gtaaaggaca ttacaaaggt cacgggttat gtgcgtggag
    10561 gctgttcacc tctcggaatg aaaaagcagt tcaagacaac cttccacatt acagcagagt
    10621 caattccgaa aatcattgtc agtgcaggaa aaatcggata tcaaattgac ctgaagcctg
    10681 aggatttaat tcgtcttaca aacggtcaat gtgcagatat tataaaggac tgacctgata
    10741 ttcaaaaaac aacacccccg actgtgccaa acggtttagt cgggggtgta ttttattaat
    10801 atgatttatt cgtcaataaa ttcaaggtct tcgtcactta cttttttgag ttccttttta
    10861 ttgaacaaat cgtaaataac gggaacgatg tagagcgtca taaatgttgc gtagagcaaa
    10921 ccgccgatgc agacaattgc gataggctgc atcatttctg cacctgtgcc tatgccgaga
    10981 gccataacaa caagaccgag aacggttgtg agtgcggtta tgaggatagg acgcatacgg
    11041 gttttgcctg cctcaacaat tgactcaatt ctttctttgc cgtcaatacg aagtctgttg
    11101 atgtagtcaa caagaacgat accgttgttt acaatgatac cgcagagcat tacaaaaccg
    11161 acaagcgata caacgctgat gtcaaatcct gtgattaaaa gtgcgaggaa gccgcccgtg
    11221 aatgcaagcg gaattgtaaa cataataatg aacggtgatt taaggctctg gaactgtgca
    11281 accataatga ggtagatgag aataatgccg agcaggagca taagcataag ctgtgaaact
    11341 gcctccattg tcattttgtc tgtacctgca t
//
LOCUS       DAOMVE010000053        11294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1365_length_11294_cov_3.949907, whole genome shotgun sequence.
ACCESSION   DAOMVE010000053 DAOMVE010000000
VERSION     DAOMVE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1365_length_11294_cov_3.949907"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1163)
                     /locus_tag="PLS64_04435"
     CDS             complement(<1..>1163)
                     /locus_tag="PLS64_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43108.1"
                     /translation="MTKAESKVIDFIKKHILLFLLVAVTIIAIFLRICGMDFQSDDFN
                     SFLNSWWSIIKLNDFTGLATQVGNYNIPYQVIIYLMTLLPLNALYAYKIVSIIFDFVL
                     AISTAMLVYSFAKNNRRLKAILTYSAVLLSATVIFNSSFWAQCDSIYTSFIILAILFL
                     HKDKPIASFVFIGIAFAFKLQTVFIIPVLLYYWISTKKISILHFFIIPAVDVIMCLPA
                     IIMGRPFIDIITIYAEQTDYGKLIQMNCPNFYALICDGNDMTYYYLFKQFSVFLTIAV
                     LGIMMCIIIYKKVDLKNLETFLLTTAWTVFTCIMFLSSMHERYGYLLDIILIVYAAVS
                     AKHLWIPIVSTLISLRGYCYYLFSYELISLKFTAIIYTALYVYITFLLVKTIF"
     assembly_gap    1164..1173
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1178..2176)
                     /gene="trpS"
                     /locus_tag="PLS64_04440"
     CDS             complement(1178..2176)
                     /gene="trpS"
                     /locus_tag="PLS64_04440"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRL43109.1"
                     /translation="MENQTKKEVVFSAIQPSGTITLGNYLGAVRNWVTMQNEYDCIYA
                     LADLHTITVRQTPAVMRKNIIDAYASIMACGIDVEKSLFFIQSHVHTHAELAWALSCY
                     TQFGELSRMTQFKDKSAKHADNINAGLFTYPVLMAADILLYQADKVPVGADQKQHIEI
                     TRNIAERFNGIYGNVFKIPDGFIPKNGARVMSLQDPTRKMSKSDENVNGCVHLLDAPE
                     VIMKKFKRAVTDSEAKVRYAEGKDGVNNLMAIYSAVTGLSYEQIEKDFDGKGYGDFKT
                     AVGEAVVEELRPIRERYEKLVADKAYLESCYRKADEIALKISSRTMGKVMKKIGFI"
     gene            complement(2192..3376)
                     /locus_tag="PLS64_04445"
     CDS             complement(2192..3376)
                     /locus_tag="PLS64_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRL43110.1"
                     /translation="MEYRFSDRVSSLKPSAIREIFKYAADPSVVSLSAGNPSPDAFPS
                     KEIAEISAKVFAEDPISVLQYSVSEGYTPLRKHLTEYMKKEHNTGSDNDDILITTGAQ
                     QIMDLCSKALVNEGDVVICEAPSFIGSLNTFRSYNAKLAGVPVEPDGMSTEKLEEALK
                     ANPNAKLIYTIPNFQNPSGVTMSLEKRKKVYELAKKYGVLIIEDNPYGDLRYSGEYVP
                     NIKSFDTDGIVIYAGSFSKVISPGMRVAYTIAPKPIFQKLVVCKQGNDVHTNIWAQYV
                     CDEFITKYDFNAHLAKLREIYTKKANFCMELLDKYCAPKITYHKIDGGLFIWCDLPED
                     IDMPNFCKELVLKKVCVVPGNAFLTDENETCHSFRINFSTPTDEQLERGIKILGEYAN
                     SL"
     gene            3732..5030
                     /locus_tag="PLS64_04450"
     CDS             3732..5030
                     /locus_tag="PLS64_04450"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HRL43111.1"
                     /translation="MSRRNDYDYFDLDNFDDSIDSREYRRRQQQRRSASYAARPSSQR
                     PVRRPVKRRNKRRTRNRIIIVASALIILFVIIFIITSIFKGCSKEDALASVPTGTVGT
                     TASPDETAKSSTANKADELSPTYFMTPSIKDDNTNGTLSYGIYVWNKIGYELFSSDDA
                     RAQNYAQTINGFADKFGSSVKVYDMIVPNHTEMGLPQRLKDSDAPSTSQADNIKAAYA
                     ALSDKVTPINCYNRLAEHNTDYIYFKSDHHWTGLGAYYAYTAFADTNDLPVLSLDDCE
                     EQKIDGFTGSFTNSADGLDSDTVSYWKFPYNVTMDITQADGSSASYNSPYYEAAEGGS
                     NTYGVFIMGDNALTVLKSASENAAAGKKIAVVKESYGNAFVPYLTNNYEEVHVIDFRY
                     FKQNLVDYCKTNGIDEVLFMNGVMSANTQVQLDSMSGLFN"
     gene            5064..5765
                     /locus_tag="PLS64_04455"
     CDS             5064..5765
                     /locus_tag="PLS64_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRL43112.1"
                     /translation="MPMIADSVKVSVFGKISDKLYSAQITSVSGRCKSAYVISHKPVT
                     EYFEGVVVAVAEFDGLDGERPIISQYGEVFYEPELRQVLSKLKNIKLKSIVCLYEKSC
                     GAVIFYKSRQNTKILLVKNSNGRYWSFPKGHIEDGENEHQTAIREIKEETGLDVVIEK
                     GFREISEYCPFGKIRKRVVFFLAQAFTDNVKIQEEEIDSYIWVDLQQARKMCSYDNDL
                     RIIEKAETAIHLLRN"
     gene            6210..6791
                     /locus_tag="PLS64_04460"
     CDS             6210..6791
                     /locus_tag="PLS64_04460"
                     /inference="COORDINATES: protein motif:HMM:NF039476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6512 family protein"
                     /protein_id="HRL43113.1"
                     /translation="MKKETVKMRLWGLVIVPLMIIVFKNLYSLTNGALLGIFFGAVNG
                     SMWEQTKVVMLSYLVWSLVEAIGKRSGFHRFAVSRIISLWFVGIFCLATYLLIYALGV
                     ESAGLPRFICAIAVCFSAFALSARLEYGERKIEQLFLPCVFMMLLLIAVYCSFTVFPP
                     HMILFEDIQTGMYGIIPEYIDSGAIALDTLYRV"
     gene            6919..7680
                     /gene="rlmB"
                     /locus_tag="PLS64_04465"
     CDS             6919..7680
                     /gene="rlmB"
                     /locus_tag="PLS64_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRL43114.1"
                     /translation="MMKNERFENTEQKPDVIAGRNPVSEAVRSNRPIDKILVAKGEKS
                     GAIVGILAKARDKKIPVKEVDRTKLDYVSGGATHQGIVAFAAAKDYSTVDDILEYAES
                     RGEAPFVVVLDEVEDPHNLGAIIRTAECAGVHGIIIPKRRSAGLSYTVAKASAGAIEY
                     MRVARVTNIAVTLDELKEKGVWVYGADMDGTDYDECDFSGACAIVIGNEGKGISRLVR
                     EKCDVIASLPMKGQINSLNASVAAGILMYKAMKNR"
     gene            7911..10571
                     /locus_tag="PLS64_04470"
     CDS             7911..10571
                     /locus_tag="PLS64_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43115.1"
                     /translation="MADMDKDRLKELEIESILEETHYLADQERMEQTAQKYRAKPKIE
                     EIFSNADKKPRLKNTNPLDESEPDTSNSIVGDKTAATMQAELIMDGNDDDLVTPEQLK
                     AEAEKKAAEKAEKAKREYEEAKKKAAEEENKIVTLTPEYDENTEFSGEVLGEVDQFRD
                     EAEIKSYHTIDIDIPEDKPKEKTETKEKKEASQTPVHQFPNTEKPPRKVVAKVPVYRP
                     DEPRNILNVKAGRFSEAVANEYEEYVRSKNPSVIAHVLRPEPTIVDEEIAPTEEKHKD
                     NRPIGEKVISALVGIFSKDESDDNDTVKEENSKPVEDYTGEEDEKSILYELNHNIRKL
                     FMRSLLSGIIAAVVVVLTIVTRIFPSAICSAVPFAPAAYAILLFILMAASLVLNRVAM
                     LSGLSPLVHIKGNSDTAVAVAGAAGMVQIIVSFFCLGDLNGFHVNYYTVIPMLAFFAN
                     NVGKLYMVLRVKDNFKFVSSKGQKYASKIYNNESVAMQMMSGTAADRPIIAYQHKTEF
                     PSNFLKISYAPDPSEDLASKLAPITTIASIIIAVMYGVVKLSFADALNAFALITAVSV
                     PVATLLSVNAPVRKLCKTLLSYGSMLSGYPSVKQFCDSTAIMIDANELFPAESISLEG
                     IKTFEDYGIDESLLCGIAILKEAQNPIANAFDSVVAETEETLPEVESVLYEDEIGLVG
                     WIKSERILVGSRTLMEKYSVEVPNMEYEEKYTSQGRQVTYLSRAGRLVAMFVTRYTPD
                     AQLKAEMQRAETNGISFLIRTTDYNVTNDLVAKLYDLFYRSIKVLPTGLGNVLREAED
                     TVEETSRSYLITNGKAASLARAVTGCVKIKHNISLSIIIQLIAVIFGLLVASTLSLYA
                     GVQVMGSLEVLIYALFWGAAAVFAPAVQKP"
     gene            10587..11234
                     /gene="rpe"
                     /locus_tag="PLS64_04475"
     CDS             10587..11234
                     /gene="rpe"
                     /locus_tag="PLS64_04475"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRL43116.1"
                     /translation="MKIAPSLLSCDFSKMGEEIERMDLAGADWMHLDVMDGHFVPNIT
                     FGAPVIKAVRKFSNRPFDVHLMISDPLKYIDDFADAGADIITFHVECDSDIDDTIAKI
                     KSRGIKPGLVLKPNTPAWTVFPYLEKLDLVLVMTVEPGFGGQSFMPEMMPKVKEIKRE
                     CQRRGLNNILIEADGGIGRDTIEQASEAGVDVCVAGTAVFRAADPAKAIELFKSF"
BASE COUNT         3305 a         2235 c         2565 g         3179 t
ORIGIN      
        1 tgaaaattgt ttttacgagc aagaatgtta tataaacata aagtgcggtg tagatgattg
       61 ccgtaaattt aaggctaata agctcgtagg agaacagata gtagcaatag cctctcaggc
      121 ttataagcgt tgacacaatc ggaatccaca aatgctttgc acttactgcc gcatagacaa
      181 ttaaaataat gtcaagcaga taaccgtagc gttcgtgcat tgacgaaagg aacatgatac
      241 aggtgaacac agtccacgct gttgtcagaa gaaaagtttc aaggtttttc aaatcaacct
      301 ttttatagat gataatgcac atcataattc cgagtacggc aattgtcaga aagaccgaaa
      361 actgcttgaa cagataataa tatgtcatat cgtttccgtc acatatgagt gcgtaaaagt
      421 tcggacagtt catctgaata agctttccgt agtcggtctg ttccgcatat attgtgatga
      481 tatcgataaa cggtctaccc ataataattg caggcaggca cattatcaca tcaaccgcag
      541 ggattataaa gaaatgcaga atcgaaattt tctttgtcga aatccagtaa taaagcagaa
      601 caggaatgat gaatacggtt tgcagcttga atgcaaacgc aatgccgatg aacacaaacg
      661 atgcaatcgg cttgtctttg tgcaaaaaca ggattgcaag gattataaac gaggtgtaaa
      721 ttgaatcgca ctgcgcccaa aacgatgagt taaaaatcac cgttgccgaa agaagtacgg
      781 ctgagtaggt gagtatcgct ttgagccgtc ggttattctt tgcaaacgaa taaacaagca
      841 ttgcggttga aattgcaagt acaaaatcga atattattga tacgattttg tacgcataca
      901 gagcgtttag cggcaaaaga gtcataaggt agattatgac ttgatacgga atgttgtagt
      961 tacccacctg agttgcaagc cccgtaaaat cattcagctt gattatgctc caccacgaat
     1021 ttaaaaagct gttgaaatca tcgctttgaa aatccattcc gcagattctg aggaaaattg
     1081 caataatcgt taccgcaacg agtaaaaata aaagaatgtg tttttttata aagtcaatga
     1141 ctttgctttc tgcttttgtc atannnnnnn nnngggttta aataaaaccg attttcttca
     1201 taaccttgcc cattgtgcgg ctgctgattt taagggcaat ttcatcggct tttctgtaac
     1261 agctttcaag atatgccttg tctgcaacaa gcttttcgta gcgttcacga atcggacgaa
     1321 gttcttcaac aactgcctcg cctacagctg tcttgaagtc gccgtagccc ttgccgtcaa
     1381 aatccttttc aatctgttca tatgaaagac ctgtaactgc gctgtaaatt gccataaggt
     1441 tatttacacc gtctttgccc tccgcatagc gaacctttgc ctcgctgtcc gtaacggctc
     1501 tcttgaactt cttcattata acctcgggtg catcaagaag atgtacacag ccgttgacat
     1561 tttcatcgga cttgctcatc tttcttgtcg ggtcctgaag actcataacc cttgcaccgt
     1621 tctttggaat gaaaccgtcc ggaatcttga acacattgcc gtaaataccg ttaaaacgct
     1681 ctgcaatgtt tcttgtaatt tcaatgtgct gtttctggtc tgcaccgaca ggcactttgt
     1741 cagcctgata gagaagaatg tcggcagcca tgagtacggg atatgtgaaa agacctgcgt
     1801 tgatgttgtc ggcgtgcttt gcagacttgt ccttgaactg cgtcattctt gaaagctcgc
     1861 cgaactgtgt gtaacagctg agcgcccatg caagctctgc gtgagtgtga acatgactct
     1921 gaatgaagaa aaggcttttt tcaacatcaa taccgcaagc cataattgaa gcataagcgt
     1981 caattatatt cttacgcata acggcaggag tctgtcttac cgtgattgtg tgcaaatctg
     2041 caagagcgta aatgcagtcg tattcgttct gcattgtaac ccagttgcga accgcaccga
     2101 gatagtttcc gagtgtaatt gttccgctgg gctgtattgc gctgaataca acctcttttt
     2161 ttgtctgatt ttccattatt cccctccgtg attaaagact gtttgcatac tcgccgagga
     2221 ttttgattcc tctttcaagc tgttcgtcag tcggtgttga aaagtttatt ctgaaactgt
     2281 gacaggtttc gttttcgtca gttaaaaatg cgttgcccgg aactacgcaa acctttttga
     2341 gaacgagttc tttgcagaaa ttcggcatat caatatcctc gggcaagtca caccaaatga
     2401 aaagaccgcc gtcaatttta tgatatgtga tttttggtgc gcagtatttg tcaagaagct
     2461 ccatgcagaa gttcgctttc tttgtgtaga tttcacgaag ctttgcaaga tgagcgttaa
     2521 agtcatactt tgtgatgaat tcatcgcata catattgtgc ccagatgttt gtatgtacat
     2581 cgttgccctg tttgcacaca acaagctttt gaaaaatcgg ctttggtgca attgtgtaag
     2641 caacacgcat acccggtgaa attacctttg agaatgaacc ggcatagatt acaattccgt
     2701 ccgtatcaaa gctttttatg ttcggaacat attcaccgct gtaacgcagg tcgccgtacg
     2761 ggttgtcctc aatgatgaga acaccgtatt ttttggcaag ctcataaact ttcttacgct
     2821 tttcaaggct cattgtaaca cccgaaggat tttgaaaatt cggaattgtg taaatgagct
     2881 ttgcgttcgg attggctttt aaagcctctt caagcttttc tgtgctcata ccgtcaggct
     2941 caacgggaac acctgcaagc tttgcgttgt agcttctgaa tgtgttgagc gagccgatga
     3001 atgacggtgc ctcgcagatt acaacatcgc cctcgtttac aagagccttt gagcataaat
     3061 ccataatctg ctgtgcgcct gttgtgatga gaatgtcgtc attgtcagag cctgtgttat
     3121 gctccttttt catatattcc gtaaggtgct ttctcagcgg agtgtaaccc tccgaaacgc
     3181 tgtactgtaa aaccgaaatc ggatcttctg caaaaacctt tgccgagatt tccgcaatct
     3241 cctttgaggg aaatgcatcg ggcgacgggt ttcctgccga aagcgacaca acgctcggat
     3301 cggcggcata tttgaaaatt tctctgatag cactcggctt caggcttgaa accctgtcgg
     3361 aaaacctgta ttccataaaa atatctcctt ttaagatgat aaggattttt taaaattcca
     3421 taccctgtaa ttctatcacg aattgcatat atgttcaaga aatttgactg tttttactgc
     3481 gattttattc aaaaaatgtg taatttatta ttttgcattt gtaaatgtta attcacggaa
     3541 attgtatttt ttgtgctttt tgtattgttt ttgatgagtt tctgttgtat aatctaagtg
     3601 ataatgtcct tacggtcaga gcaaggcttg ctttgattca aacaggagta cactgcgagt
     3661 aattaataca cttatataat tcattgaatt caaattgaat ttaaaaatct tcagtttggg
     3721 agagtaacac tatgagcaga agaaacgatt acgattattt tgatcttgat aatttcgatg
     3781 acagcattga ttcccgtgaa tatcgtcgca gacagcaaca gcgccgttct gcaagctatg
     3841 ccgcaagacc gtcatcacag cgtccagtca gaagacctgt aaaaagaaga aacaaaagaa
     3901 gaaccagaaa cagaattatt atcgttgcga gcgcattgat aattctgttt gttattattt
     3961 ttattataac ttcaattttt aagggttgtt caaaggaaga tgcccttgca agcgttccca
     4021 cgggaacagt gggtacaacg gcatctcccg acgaaaccgc aaaatcttcg acagctaaca
     4081 aagcagacga gctttctcct acatacttta tgacaccgag cataaaggac gacaatacaa
     4141 acggaactct ttcgtacggc atttatgtgt ggaacaaaat aggctacgaa ttgttcagca
     4201 gtgatgatgc aagagcgcag aactatgctc agacaatcaa cggctttgca gacaagttcg
     4261 gcagttccgt aaaggtttat gatatgattg ttcctaacca taccgaaatg ggacttccgc
     4321 aaaggctcaa agacagcgac gctccgtcaa cctcacaggc tgacaacatc aaggcggctt
     4381 atgcggctct ttccgataag gtaactccga ttaactgcta caaccgtctt gcggaacata
     4441 atacagatta catctacttt aagagcgacc accattggac aggcctcggc gcttactatg
     4501 cttacacagc atttgccgat acaaacgatt tgcccgttct ttcgcttgat gattgcgagg
     4561 aacagaagat tgacggcttt acaggctcgt ttaccaactc ggcagacggt cttgacagcg
     4621 atacggtaag ttattggaag ttcccttata atgtaacaat ggatatcaca caggcagacg
     4681 gttcttccgc aagctataac agtccctact atgaagccgc agagggcggt tcaaacacct
     4741 acggtgtgtt tattatgggt gataatgccc tcacggttct gaaatcagct tcggaaaatg
     4801 cggcggcagg caagaagatt gccgttgtaa aggaaagtta cggcaacgct tttgttccgt
     4861 atctcaccaa taactacgaa gaagttcatg ttatcgattt cagatatttc aaacagaatc
     4921 ttgttgatta ctgcaaaacc aacggaattg acgaggtgct ttttatgaac ggtgtaatga
     4981 gtgccaacac tcaggttcag cttgacagta tgagcggact ttttaactga tatcatatcg
     5041 ggtaaattct tgtaaggagg tgtatgccga tgatagcaga cagcgtgaag gtgagcgtgt
     5101 tcggaaaaat aagcgataag ctgtattcgg cacaaattac ctccgtttca ggacgctgta
     5161 aaagtgcgta tgtgatttcg cacaaacccg taacagaata ttttgagggc gttgtggttg
     5221 ccgttgccga atttgacggc cttgacggcg agcgtccgat aatctcacaa tacggtgagg
     5281 tgttttatga gcccgaactg cgacaggtgc tttcaaaact gaaaaatatt aagctgaaaa
     5341 gcattgtgtg cctttacgaa aagtcgtgcg gtgcggttat cttctacaaa agcagacaga
     5401 acacgaaaat tctgcttgta aagaacagca acggcagata ttggagcttt ccgaaggggc
     5461 atattgaaga cggtgaaaac gaacatcaga cagcaatccg tgaaattaaa gaggaaacag
     5521 gtcttgatgt cgtgattgaa aagggctttc gtgaaatcag cgaatactgt cctttcggta
     5581 aaattcgcaa acgggttgtc tttttccttg cacaggcatt tacggataat gtcaaaattc
     5641 aggaggagga aattgacagt tatatttggg ttgatttgca acaggcacga aaaatgtgct
     5701 cctatgataa tgatttgagg attattgaga aagctgaaac ggcgattcat cttctcagaa
     5761 actaaaagcc gacaatttaa cacgaaattt acaggtacac ggcggtgtgc ctgttttttt
     5821 gattttataa aacattttct gtaaattaaa aatgttgaaa aaaagcaaaa aataccactt
     5881 gacatgtgga ttatacagaa tgtaatctaa tttccccgtt acaatcctgt atccaaaaat
     5941 gtggaaaacc tattttttta atgttaaaag ttacaacttt gtaaatttcg tgtagtatcg
     6001 ggatttttta acatcacttt caacactttc aacatagttt tccacattga tgtttaaaat
     6061 gttgagttat acagaatgta taagtgcaaa tttagaaaaa taaatcgcaa attcgtgatt
     6121 caaaaaaaca tttttttctg cgattcaaat tgtggcaagg taatatatga caatctatac
     6181 tcataaaata catcggcgag gtgtgtttta tgaaaaagga aaccgtgaaa atgaggcttt
     6241 gggggcttgt cattgttccg ctgatgataa ttgtgttcaa aaatctttat tcgctgacaa
     6301 acggtgcgtt gctcggcata tttttcggtg ctgtaaacgg cagtatgtgg gagcagacca
     6361 aggttgttat gctttcctac cttgtgtgga gtcttgttga ggcaatcgga aaacgctcgg
     6421 gattccatcg ctttgccgtg tcaagaataa tttcgctgtg gtttgtcgga atattctgtc
     6481 ttgcgacata tctgctaatc tatgcactcg gtgttgaaag tgcaggtctg ccaaggttca
     6541 tttgtgcaat tgccgtgtgt ttttctgcgt ttgcactcag cgcaaggctt gaatacggtg
     6601 aaagaaaaat tgaacagctt tttttgccct gcgtgtttat gatgttgctt ttgattgccg
     6661 tgtactgcag ttttacagtg ttccctccgc acatgatact ttttgaggac atacaaacgg
     6721 gaatgtacgg cataattccc gaatacatcg actccggagc gattgctctt gatacattgt
     6781 acagagtttg agtttttaaa aacaaatggc ttaaaagtat ttaataattt gaattgttat
     6841 gttataatag atagcaaaat gaagtggtgt gtcccatgca gacacaaaaa ataaaaagga
     6901 aaccgaataa ggctgattat gatgaaaaac gaaagatttg aaaatacaga acaaaagcct
     6961 gatgtgattg cagggcgaaa tcctgtaagc gaggctgttc gcagtaaccg accgattgac
     7021 aaaattcttg ttgcaaaggg tgaaaagagc ggtgcgattg tgggaatcct cgcaaaagcc
     7081 cgtgacaaga aaattcccgt taaggaagtt gaccgcacaa agcttgatta tgtgagcggc
     7141 ggcgcaactc atcagggtat tgtcgcattt gcggcggcaa aggattattc aacggttgac
     7201 gatatcctcg aatatgccga aagcaggggc gaagctccgt ttgttgttgt gcttgatgag
     7261 gttgaagatc cgcataatct cggtgcaata atcagaaccg cagaatgtgc cggagttcac
     7321 ggaattatta ttccgaaacg cagaagtgcc ggtttaagct acacggttgc caaagcgtct
     7381 gcaggcgcaa ttgaatatat gcgtgttgca agggtgacca atattgcagt tacacttgat
     7441 gaactcaaag aaaaaggcgt gtgggtttac ggcgcagata tggacggcac ggattatgac
     7501 gagtgtgatt tttcgggtgc gtgtgcaatt gttatcggca acgaaggtaa gggcatttca
     7561 agacttgtcc gtgaaaagtg cgatgtaata gcgtcattgc ccatgaaagg tcagataaat
     7621 tcccttaatg cgtcggttgc cgcaggaatt ttgatgtaca aagcgatgaa aaaccgctga
     7681 tgtcaaattg aaaaatgtaa aatggaaaat ttataaaagt acatgtatgt aggggcgttc
     7741 attggacgcc cgcattgatg aaattttatg tttaaataaa atcatttgat gttatcaaag
     7801 ttgtgtgttt aaatgtcaaa gattgtaaaa acagatagca acgctcgggc ggcatagttt
     7861 ccgataaatc taactatctg cccgagagta aataaaaagg agtgaatgct ttggctgata
     7921 tggacaagga caggcttaaa gagcttgaaa tcgagagcat actcgaagaa acccactatc
     7981 ttgccgatca ggaacgaatg gagcagacgg cacagaaata ccgtgcaaag ccgaagattg
     8041 aagagatttt cagcaatgcc gataaaaagc cgaggttaaa aaatacaaat ccgcttgatg
     8101 aatccgaacc tgatacttca aactcaatag tcggggataa gactgccgcc acaatgcagg
     8161 cagagcttat aatggacgga aatgacgatg accttgtaac tcccgaacag cttaaagcag
     8221 aggctgaaaa aaaggctgcg gaaaaagccg aaaaagccaa gcgtgagtat gaggaggcaa
     8281 agaaaaaagc cgccgaggaa gaaaataaga tagttaccct cacacctgag tatgatgaaa
     8341 atacagaatt ttcgggcgaa gtgctcggag aggttgacca gtttcgtgac gaggctgaaa
     8401 ttaaaagcta ccataccata gatatcgaca ttcccgagga taagccaaag gaaaagaccg
     8461 aaaccaaaga aaagaaagag gcttcgcaga ctcccgttca tcagtttccg aatacagaaa
     8521 aaccgcctcg caaggtggtt gcaaaagttc ccgtgtatcg tcccgatgaa ccgagaaata
     8581 ttctcaatgt caaggcaggc agattttccg aggctgtagc gaatgagtat gaggagtatg
     8641 tgcgttcaaa gaatccttcg gtaatcgctc atgtgcttcg tcccgagccg acaatcgttg
     8701 atgaggagat tgccccgacc gaggaaaagc ataaggataa ccgccctata ggcgaaaagg
     8761 ttatttctgc ccttgtcgga atcttttcaa aggatgaatc cgacgataac gataccgtaa
     8821 aagaggaaaa ttcaaagcct gttgaggatt atacaggcga ggaggacgaa aaaagtattc
     8881 tctatgagct gaatcataat ataagaaagc tctttatgag aagtctgttg tcgggaataa
     8941 ttgccgcagt tgtggttgtt ctcacaatcg ttacaagaat ttttccgagt gcaatatgtt
     9001 cggcagttcc ttttgcaccg gcggcatacg caattttgct gtttattctt atggcggcgt
     9061 cgcttgtgct gaaccgtgtg gcaatgctca gcggactttc accgcttgtg catatcaaag
     9121 gtaattccga tacagccgtt gccgttgcag gtgcggcagg aatggttcag ataattgtgt
     9181 cattcttctg ccttggcgat ttgaacggat ttcatgtgaa ctactacacg gtaattccta
     9241 tgcttgcgtt ctttgcaaat aatgtgggaa agctttatat ggttcttcgt gtaaaggaca
     9301 attttaagtt tgtttcgtca aagggacaga aatatgcgtc aaaaatttac aacaacgagt
     9361 ctgttgcaat gcagatgatg agcggtactg ccgccgatcg tccgataatt gcctatcagc
     9421 ataagaccga atttccgtca aactttctca aaatatctta tgcacccgat ccgagcgagg
     9481 atttggcgtc aaagcttgcc ccgataacaa caattgcatc aataatcatt gcggtaatgt
     9541 acggtgttgt taagctgtca tttgcagacg ctttgaatgc atttgcactt atcactgcgg
     9601 tgtctgtgcc tgtggcaaca cttctttctg ttaatgcccc cgtgagaaag ctctgcaaaa
     9661 ctctgctttc ctacggctca atgctttcgg gttatccgtc ggtaaagcag ttctgcgaca
     9721 gtacggctat aatgatagat gcaaatgaat tgttccctgc cgagtcaatc tcgcttgagg
     9781 gcatcaaaac ctttgaggat tacggcattg atgaatctct tctctgcgga attgcaattc
     9841 ttaaagaggc tcagaatccg attgcaaatg cttttgattc ggtagttgca gaaaccgagg
     9901 aaacacttcc cgaggttgaa agcgttcttt atgaggatga aatcggactt gtgggatgga
     9961 ttaagagcga aagaatcctt gtgggttcac gcacgcttat ggaaaagtac agcgtggaag
    10021 ttccgaatat ggaatatgaa gagaaatata cttctcaggg cagacaggtc acatatctgt
    10081 caagagcagg cagacttgtt gcaatgtttg ttacaagata cactcccgac gctcagctca
    10141 aggctgaaat gcagagagcc gaaaccaacg gcataagctt cctcatcaga acaaccgatt
    10201 ataatgttac aaatgacctt gttgcaaaac tctacgattt gttctaccgc agtatcaagg
    10261 tgcttccgac aggactcgga aatgttctca gggaggctga ggatacggtt gaggaaacct
    10321 cccgttcata tctcattaca aacggcaaag ccgcatcgct tgcccgtgca gttacgggtt
    10381 gcgtaaaaat caagcacaat atttcgcttt caatcataat tcagctgatt gccgtaattt
    10441 tcggattgct cgttgcgtca acactttcgc tttatgccgg tgtacaggtt atgggttcgc
    10501 ttgaagtatt gatttatgcg ttgttctggg gcgccgccgc tgtttttgct ccggctgtcc
    10561 aaaaaccgta ggaaaggatg taaaatatga aaatagctcc gtcacttttg tcttgtgact
    10621 tttcaaaaat gggtgaagaa attgaaagaa tggatctggc gggcgccgat tggatgcatc
    10681 ttgatgttat ggacggtcac tttgttccga acataacctt tggcgctccc gtaatcaagg
    10741 cggtaagaaa gttctcaaac cgcccgtttg atgttcacct tatgataagc gatccgctta
    10801 aatatattga cgattttgcc gatgcaggtg cggatatcat caccttccat gttgaatgtg
    10861 actctgacat tgatgataca attgcgaaaa tcaagtcaag aggaatcaaa cccggacttg
    10921 ttctaaagcc aaacactcct gcctggacag tatttccgta tcttgaaaag cttgaccttg
    10981 tacttgttat gacggttgaa ccgggctttg gcggacagag ctttatgccc gagatgatgc
    11041 cgaaggttaa ggaaatcaag agggaatgcc aacgcagagg tctgaacaat atccttattg
    11101 aggctgacgg cggaatcggc agagatacca ttgagcaggc gtcagaggca ggcgttgatg
    11161 tgtgcgttgc gggaacggct gttttcagag ccgcagatcc cgcaaaagca attgagcttt
    11221 ttaaatcatt ctgataaatt ctgataataa gcaagcccga atccgatatt tctcgaattc
    11281 gggcttttgt tttt
//
LOCUS       DAOMVE010000054        10813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1465_length_10813_cov_3.082357, whole genome shotgun sequence.
ACCESSION   DAOMVE010000054 DAOMVE010000000
VERSION     DAOMVE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1465_length_10813_cov_3.082357"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..886
                     /locus_tag="PLS64_04480"
     CDS             2..886
                     /locus_tag="PLS64_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRL43117.1"
                     /translation="MIRSMTGFGRCETVINGREITVEIKSVNHRYFEFSCRTPRSYGF
                     LDDKLKNYVNSRVSRGKIDMFVSIGASDEEPCDVTVNHQLVSGYINAFKEISEKYDIP
                     NDVSTVSLSRFPDVFTVHKAPEDEDQITADVLSAAKIAVDAFVAMRETEGEKMKEDIL
                     SRANTILSVVGEIEERSPQTVAEYEQRLMERIKQTLAENDVKVDEQRVLTEVAVFADK
                     VAVAEETVRLRSHFDQLSKFLEYDEPVGRKIDFIIQEMNRESNTIGSKVQDAILAHKV
                     VDIKSEIEKIREQVQNIE"
     gene            896..1153
                     /locus_tag="PLS64_04485"
     CDS             896..1153
                     /locus_tag="PLS64_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRL43118.1"
                     /translation="MKLINIGFGNLVSSDKIVAVVAPDSAPVKRIVQDAKQNNLLIDA
                     TCGRKCKSVIVAENNHVILSAISCEAIQNRTDESEENKDEH"
     gene            1143..1724
                     /gene="gmk"
                     /locus_tag="PLS64_04490"
     CDS             1143..1724
                     /gene="gmk"
                     /locus_tag="PLS64_04490"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRL43119.1"
                     /translation="MSINRKGNLILFTGSSGVGKGTIIKELLDRDKNIRLSVSNTTRE
                     PREGEIDGVHYNFLTREQFNDVLKKDGYLEHAEFCGNLYGTPKKQVEDMLNQGYDVLL
                     EIEVKGGLQILDKYPDILSIFILPPSMESLERRLRRRGTEDEETIRKRLAQAAEEISY
                     KDRYKYNVVNGDLETAINEVLDILHKEDENLIK"
     gene            1770..1976
                     /gene="rpoZ"
                     /locus_tag="PLS64_04495"
     CDS             1770..1976
                     /gene="rpoZ"
                     /locus_tag="PLS64_04495"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRL43120.1"
                     /translation="MHKVDVDSLLQGKQSKYALVVGVAKRAREITQTFEEEQIVTEDK
                     PVLIAIKEIEGHEINILEPDEDEL"
     gene            1936..4413
                     /gene="priA"
                     /locus_tag="PLS64_04500"
     CDS             1936..4413
                     /gene="priA"
                     /locus_tag="PLS64_04500"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRL43121.1"
                     /translation="MKSTFLSLTKMNSDLTVKVAVENTTYVFDKLFDYLVPSAFTDLV
                     QVGKRVLIPFGRGNKKKQGIIFELSPVSDDIPVEKLKSICSVLDDEPVLSKELLKLAE
                     FMKEHYFCTYYDAVKTMLPAGINYKITTLYGVREGVDEEELSEEQQRIFAYLHSKRKA
                     VKSDKILDDFGLDNTVILEDMVKSGYLYKSDEAFRKVSDAVMKMAAPTELADSDNIKL
                     TEKQKAVLDLIKMTGGTSVKEVCYFTGVTTGVTDALYKKGLIYYYDEEVFRIEDRTKD
                     ESLAPVALSKQQQTACDNLYAEYADSKPHTSLLFGVTGSGKTSVFMKLIERVINDNKG
                     IIVMVPEISLTPQFVSTFSKRFGEQIAVFHSALSLGERLDEYKRVKKGLAKIVIGTRS
                     AVFAPFEKVGLIIMDEEQEYSYKSESSPRYHAREIAKFRCSYDNALLVLSSATPSVES
                     YYYAKNGRYSLNTLTERYGDAVLPKVVVADMNVEQQNGNVTGFSETLLENLRYNLENN
                     KQSILLLNRRGYNTFVTCRMCGEPVVCPNCSISLTYHSANRRMMCHYCGYSVPYTEEC
                     PSCHSKSLRFGGTGTQKAESEISELFPDARILRMDTDATSSKSSYEKMITAFADGKYD
                     ILVGTQMVAKGLNFPNVTLVGVLNTDYMLYADDYRSYERTFSLLTQVVGRSGRGASKG
                     MAVIQSYTPDNLIISMAARQDYLAFYSTEIQVRKAMLYPPFADICLIGFSGEKQQLTM
                     KAANSFLQCFVSHARSEYPAMPLRILGPSPANVVKVSNKFRYKLIIKCKNNRDFRGLL
                     SAVMTEFGKNKEFGKVSTYADMNALTF"
     gene            4430..4906
                     /gene="def"
                     /locus_tag="PLS64_04505"
     CDS             4430..4906
                     /gene="def"
                     /locus_tag="PLS64_04505"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRL43122.1"
                     /translation="MALRQIVKEGDSVLTKKCRPVVKFDDRLADLIDDMIETLHKAEG
                     VGLAAPQVGILRRVVVIDVGEGPIELVNPKIIAYSGKQETLEGCLSIPGKWGYTVRPD
                     YVKVKAQDRHGDEFVIDGKDLLAKAFCHELDHLEGILFTQVATRMLTPAEIEEMNS"
     gene            4922..5839
                     /gene="fmt"
                     /locus_tag="PLS64_04510"
     CDS             4922..5839
                     /gene="fmt"
                     /locus_tag="PLS64_04510"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019679045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRL43123.1"
                     /translation="MKIIYMGTPDFAVPALKALANSEHEVCAVFTQPDKPRGRKMIMT
                     PPDVKVCAEKLNIPVYQPETFKDGKPLEIINKYNPDVIVVAAYGKILPKSVLDSAKYG
                     CINLHGSLLPKYRGASPIQQSVLNGDRETGVTAMQMDVGLDTGDILKVVKTEIGVNET
                     SGELFDRLSLMGGELILDTLSALEKGEITPIKQDESLATHTSKIDKSLCPIDFTKPAF
                     EVHNKIRGLNPWPIATTEINGKKIKVHSSLLCEKSGKAGTVISTKPLIVACGEKSVEL
                     CEIQPEGKKKMTAEAFLAGHRLSVGDVIG"
     gene            5882..6586
                     /locus_tag="PLS64_04515"
     CDS             5882..6586
                     /locus_tag="PLS64_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRL43124.1"
                     /translation="MGFFYGFDMTYLVFIVPCIIITLICQVKVKTTFSKYSQIRNSRN
                     MTGAQAAEYVLRQHGVTGVRIEHVAGSMTDHFDPRTNVIRLSDTVFNSTSVAAVGVAC
                     HEAGHAVQHAENYLPNRIRSFILPVARLGSSLSWIFIIVGFCFTQRVGFVFLYIGIIL
                     FALSVLFTIATLPVEFNASKRALETIRNGDLLYGEEYTGAKRVLQAAAMTYVAAALTA
                     IMQLLRLIIIANNRRR"
     gene            6605..7945
                     /gene="rsmB"
                     /locus_tag="PLS64_04520"
     CDS             6605..7945
                     /gene="rsmB"
                     /locus_tag="PLS64_04520"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HRL43125.1"
                     /translation="MANTRNIALNVLLKIENEGAYSNIALNNAIKENRLSGVDSSFVS
                     ALVYGVLERRITLDYIIRSYSKIPLRKIETKTKNILRLGILQLLFMDKVPDSAAVNES
                     VNLAKKHKLQKSSGFINGILRNITRAEDKYTLPDEKDRARYLSVKYSVPENIVKLWIN
                     SYGENNAEQLLASLNGRAKICCRVNTLRTDADTLIKKLSDEGVVAEKIPFIDNALYLE
                     NTGSVERLRAYKSGLFHIQDASSQLCVNFLDAKPRNIMLDVCSAPGGKSFSAAQYMNN
                     RGRIFSCDMFDHKLKLISAGAKRLGITCISTLLRDASTNDTALPVADRILCDVPCSGL
                     GIMSRKPEIRYKDDLISNDLPDLQYRILCASADNLAVGGKLVYSTCTLNPDENNKNTK
                     RFLEEHPDFYGIPLKLPNGITRAFDEEPYEITLMPHTCGTDGFYISLFGKKASN"
     gene            7942..8985
                     /gene="rlmN"
                     /locus_tag="PLS64_04525"
     CDS             7942..8985
                     /gene="rlmN"
                     /locus_tag="PLS64_04525"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRL43126.1"
                     /translation="MNNLTDIKSMTLDELTEFVTENGFPKFRAKQIYDWLYKNVTDFD
                     NMRNIPADLKAFLKSSSYISVANIEKKLVSRYDKTVKYLFSFNDGECVESVVMSYKHG
                     YSICISTQVGCKMGCTFCATGKSGFSRSLAPSEMLGQIEAAQRDLNIRISNIVLMGMG
                     EPLDNFDNVVKFLRLVSSDNGLNIGMRHITLSTCGIVPKIYELAKLHLGITLSVSLHA
                     PNDEIRQRTMPIARKYSIEELLKACSDYFKTTGRRVTFEYSMISGVNDSDENARELAK
                     RLEGTQSHVNLIPVNTVEGTGYLKSNIKRQQAFINILAAKNIGATVRRTLGSDINASC
                     GQLKRKHIEEGGK"
     gene            8987..9712
                     /locus_tag="PLS64_04530"
     CDS             8987..9712
                     /locus_tag="PLS64_04530"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HRL43127.1"
                     /translation="MEVFSKSDIGLVRNQNQDDCRFGVISPSCVWAVVCDGMGGANGG
                     NIASATAVDYISTKITDLYKDDMTKEQIGELMAEIVVNANMKVFEMSMKDPELTGMGT
                     TCEFVFVKDTTVHVVHVGDSRTYAIRGGKIKQLTEDHSVVQEMVRRGELTYEQAQNHP
                     NKNFITRALGIKPSVRLDYIEANFIYGDVLLICTDGLSNCVTTGDMVKICHENRGEGL
                     ITKLVDKAKDGGGSDNITATVIY"
     gene            9731..>10813
                     /locus_tag="PLS64_04535"
     CDS             9731..>10813
                     /locus_tag="PLS64_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRL43128.1"
                     /translation="MDKYLGKRLDGRYEIHELIGVGGMANVYRCTDTVDDREVAVKIL
                     KDEYLNNEEFIRRFKNESKAIAMLSHPNIVKVYDVSFGDMIQYIVMEYIDGITLKEYI
                     DQQGIIEWKDAIHLTAQILKALQHAHECGIVHRDIKPQNIMLLQDGTIKVTDFGIARF
                     SDKSTRTMTEQAIGSVHYIAPEQARGDATDGKTDIYSVGVMLYEMLTGKLPFDGDSAV
                     TIALMQLQSTPKHPREINPGIPIGLEQITMKAMEKLPSDRYTSAAEMLSDIERFRLNP
                     SIVFDYGNKSFVDNQPTKFVHSLRDSRVRNKIDNEATKVVDMPQDDVVVKEEQFADED
                     FVKEHKPSYYAVKGIVIAAVAVCAIFF"
BASE COUNT         3336 a         2109 c         2448 g         2920 t
ORIGIN      
        1 aatgatcaga tctatgacgg gtttcggaag atgtgaaacc gttatcaacg gtcgggagat
       61 taccgttgaa atcaaaagcg tgaatcaccg ctattttgaa ttttcctgca gaaccccaag
      121 aagttacgga tttcttgacg acaaacttaa aaactatgta aattcaaggg tttcacgagg
      181 caagattgat atgttcgtat caatcggtgc gtccgatgaa gaaccctgtg atgtaactgt
      241 taatcatcag cttgtttcgg gatatatcaa cgcttttaag gaaatcagcg aaaagtacga
      301 tatcccgaat gatgtttcaa cagtttcact ttcacgcttt cccgatgttt tcacggttca
      361 caaagcgcct gaggacgagg atcaaattac tgccgatgtt ctttcagccg caaagattgc
      421 agttgatgct tttgttgcaa tgcgtgaaac tgagggcgag aagatgaaag aggacattct
      481 ttcaagagcc aacacaattc tttctgttgt aggtgaaatt gaggagcgtt ctccgcagac
      541 tgttgccgag tatgaacaaa gacttatgga acgcatcaag cagacgcttg ctgaaaatga
      601 tgtcaaggtt gacgagcaga gggttctcac agaggttgcc gtgtttgcgg ataaggtagc
      661 cgttgcggag gaaaccgtaa gacttcgcag ccactttgac cagctttcaa agttccttga
      721 atatgacgag cctgtcggca gaaaaattga ctttatcatt caggaaatga accgtgagtc
      781 aaatacaatc ggttcaaagg ttcaggatgc aattcttgca cataaggttg ttgacatcaa
      841 gagtgagatt gaaaaaatcc gtgaacaggt tcagaatatc gaataaggtg aacagatgaa
      901 gcttattaat atcggttttg gtaatcttgt cagctccgac aagattgttg cagtagttgc
      961 tccggactct gctcccgtaa agcgaattgt tcaggatgca aaacagaaca atctgcttat
     1021 tgatgcaacc tgcggacgaa aatgcaaatc ggtgattgtt gccgagaata atcatgttat
     1081 tctctcggca atttcatgtg aggcaattca gaacagaacg gacgaaagtg aggaaaacaa
     1141 ggatgagcat taacagaaaa ggaaatctta ttttgtttac cggttcatcg ggtgtaggca
     1201 agggtacaat aatcaaggaa cttcttgacc gtgacaaaaa tatccgtctt tcggtttcaa
     1261 acacaacaag agaaccgaga gagggagaga ttgacggggt tcattataac tttttgacaa
     1321 gagaacagtt caacgatgtt ctgaaaaaag acggctatct tgaacatgca gagttttgcg
     1381 gtaatctcta cggtacacct aaaaagcagg ttgaagatat gctcaatcag ggttatgatg
     1441 ttctgcttga gattgaggtc aagggcggac ttcagattct tgacaaatat cccgacattc
     1501 tcagtatatt cattcttccg ccttcaatgg agtcgcttga aagacgactt cgcagaagag
     1561 gtacagagga tgaagaaact attcggaaaa ggcttgcaca ggctgccgag gaaatcagct
     1621 acaaggacag atataagtac aatgtggtaa acggcgacct tgaaacagca ataaatgagg
     1681 tcctcgatat tcttcataaa gaggatgaaa acctcattaa ataatataca aagaaaatta
     1741 acataaaata ttttaagagg agaattatta tgcataaagt tgatgtggat tcattattac
     1801 agggtaagca gagtaagtac gcacttgttg taggcgttgc aaaaagagca agagaaatta
     1861 ctcagacttt tgaagaggaa cagattgtaa cagaggataa gcctgttctt atcgcaatca
     1921 aggaaatcga aggtcatgaa atcaacattc ttgagcctga cgaagatgaa ctctgattta
     1981 acagtcaaag ttgccgttga aaacacaaca tatgttttcg acaaattatt tgactacctt
     2041 gtaccgtctg cctttaccga tttggttcag gtcggcaaaa gggttctcat tccgttcggc
     2101 agaggcaaca aaaagaaaca gggaataatt tttgagcttt ctcctgtgag tgatgacatt
     2161 ccggttgaaa agctcaaaag catttgttcc gttcttgatg acgaacctgt cctgtcaaaa
     2221 gaacttttga agcttgccga gtttatgaaa gagcattatt tctgcaccta ttatgatgcg
     2281 gtaaaaacaa tgctccctgc gggtataaat tacaaaatca caacgcttta cggtgttcgt
     2341 gagggtgtag atgaagaaga gctttccgag gaacagcaaa gaatttttgc ttaccttcat
     2401 tcaaaaagaa aagccgtaaa gtccgataaa attcttgacg atttcggatt agataacacg
     2461 gttattcttg aagatatggt aaagtcgggc tatctttaca aatcagatga ggctttcaga
     2521 aaggtttcgg atgcggttat gaaaatggct gctccgaccg aacttgccga cagcgacaat
     2581 atcaagctta ccgaaaagca gaaagccgtt ctcgacctta tcaaaatgac aggcggaact
     2641 tccgttaaag aggtttgtta cttcacgggt gtcacaacag gcgttacgga cgcactttat
     2701 aaaaaaggtc ttatctacta ctatgacgaa gaagtgttcc gtattgagga cagaacgaag
     2761 gacgaaagtc ttgcccctgt tgcgttgtca aaacaacagc agacagcctg cgataacctc
     2821 tatgccgaat atgccgattc aaaaccgcat acaagccttt tgttcggtgt tacaggttcg
     2881 ggaaaaacaa gcgtttttat gaaactgatt gagcgtgtaa taaacgataa caagggaatc
     2941 atcgttatgg tgcctgaaat ttcgcttaca ccgcagtttg tttcaacctt ttcaaagcgt
     3001 ttcggcgagc agattgccgt atttcacagc gcactttcac tcggtgaaag gcttgatgaa
     3061 tacaagcgtg taaaaaaagg tcttgcaaaa atcgttatag gaacacgaag tgccgtgttt
     3121 gctccgtttg aaaaagtcgg tcttatcata atggacgagg aacaggagta cagctataag
     3181 tctgaaagct cccccagata tcatgcaaga gaaatcgcaa aattcagatg cagctacgat
     3241 aatgccttgc ttgttttaag ttcggcaact ccgtcggttg aatcctacta ctacgcaaaa
     3301 aacggaagat attcgcttaa cacgctcacc gagagatacg gcgatgcggt tttgccgaag
     3361 gttgttgtcg cagacatgaa tgttgaacag caaaacggca atgtaacggg attttccgaa
     3421 accttgcttg aaaatctgcg ctataatctt gaaaataaca agcagtcaat tctgctttta
     3481 aacagacggg gatacaacac ctttgtaacc tgccgaatgt gcggtgaacc tgttgtgtgt
     3541 cccaactgtt caatatcgct cacatatcac agcgccaacc gcagaatgat gtgtcactac
     3601 tgcggatatt ccgtgcctta cacggaagaa tgtccgtctt gtcacagcaa atctttaaga
     3661 tttggcggaa ccggcactca aaaggctgaa agtgaaattt cagagctttt tcccgatgca
     3721 agaatattgc gtatggatac cgacgcaaca tcttcaaaat catcctatga aaagatgata
     3781 acggcatttg cagacggtaa gtacgatatt cttgtgggaa cacaaatggt tgcaaaggga
     3841 ctcaattttc ccaatgtgac gcttgtgggt gtcctcaaca ctgactatat gctgtatgcc
     3901 gatgattaca gaagctacga aagaacattt tctcttttaa ctcaggtagt cggcagaagc
     3961 ggcagaggtg cgtcaaaggg tatggctgtt attcagtcct acactcccga caacctaata
     4021 atctctatgg cggcaaggca ggattatctt gccttttaca gcaccgaaat tcaggtcaga
     4081 aaagcaatgc tttatccgcc ttttgcggat atctgtctta tcggcttttc gggtgaaaaa
     4141 cagcagctta caatgaaagc ggctaacagc tttttgcagt gctttgtcag tcacgcaaga
     4201 agcgaatacc ctgccatgcc tttgagaata ctcggtccgt cacccgcaaa tgttgtgaaa
     4261 gtgagcaata aattcagata taaacttata ataaaatgta aaaataaccg tgatttcaga
     4321 ggactgctct cagcggttat gacagaattc ggaaagaata aggaatttgg aaaggtcagt
     4381 acatatgcgg atatgaacgc tctgaccttt tagcgaaagg atataattta tggctttaag
     4441 acaaattgta aaagagggcg acagcgttct cacaaaaaaa tgcagacctg tcgtaaagtt
     4501 tgatgaccgc cttgcagatc ttattgacga tatgatcgaa accttgcaca aggctgaggg
     4561 agtgggactt gctgctcctc aggttggaat tttaagaaga gttgtggtta ttgatgtcgg
     4621 cgaaggtccg attgagcttg taaacccgaa aatcatcgct tacagcggca agcaggaaac
     4681 tcttgagggt tgtctttcaa tccccggcaa gtggggttac acagtaagac ctgattatgt
     4741 aaaggttaag gctcaggaca gacacggtga cgaatttgtt attgacggca aggatttgct
     4801 tgcaaaggct ttttgtcacg aactcgacca tcttgaggga attctcttta ctcaggttgc
     4861 aacaagaatg ttgactcctg ccgaaattga agaaatgaat agctgataaa caggtgaaga
     4921 tatgaaaata atttatatgg gtactccgga ttttgccgta cctgcactca aggctcttgc
     4981 aaattcggaa catgaggttt gtgccgtttt tacacaaccc gacaagccga ggggaagaaa
     5041 gatgataatg actcctcccg atgtcaaggt gtgcgcagaa aagctgaaca ttcccgtgta
     5101 tcagcccgaa acctttaagg acggcaaacc tcttgaaatt atcaataaat ataatcccga
     5161 tgtcattgtt gttgcggctt acggaaaaat tcttcccaag tcggttcttg actctgcaaa
     5221 gtacggctgc atcaatcttc acggctcgct tttgcctaaa tacagaggcg catctcctat
     5281 tcagcagagc gtattaaacg gtgacaggga aaccggtgta acggctatgc aaatggatgt
     5341 cggacttgac acaggtgaca ttctgaaagt tgtaaaaacg gaaattggtg tgaacgaaac
     5401 ctcgggagag ctttttgacc gtctttctct tatgggcgga gagcttattc ttgacacact
     5461 ttctgctctt gaaaagggcg aaatcactcc gattaagcag gacgaaagcc ttgcaactca
     5521 cacttcaaaa attgacaaat cactttgtcc gattgatttt acaaaaccgg cctttgaggt
     5581 gcataataaa atcagaggac ttaatccgtg gccgattgcc acaaccgaaa ttaacggtaa
     5641 aaagataaag gttcattctt ctttgctctg tgaaaagagc ggaaaagcgg gaactgtaat
     5701 cagcacaaaa ccgctgattg ttgcctgcgg tgaaaagtcg gttgagcttt gtgaaattca
     5761 gcctgagggc aaaaagaaaa tgaccgccga ggcattcctt gccggccaca ggctttctgt
     5821 cggcgatgta atcggttaaa aattcccgtc tgcaatatgc agacagaaag gtgtttgatt
     5881 tatgggcttc ttttacggat ttgatatgac ctatcttgtg tttattgttc cttgtatcat
     5941 aataacattg atttgtcagg tcaaagttaa aacaactttt tcaaaatatt cacagataag
     6001 aaattcaaga aatatgacag gcgctcaggc ggctgaatat gttcttcgtc agcacggtgt
     6061 aacaggtgtc agaattgaac atgttgcagg cagtatgaca gaccattttg acccgagaac
     6121 aaatgtaatc agactttccg acacggtttt taacagcacc tccgttgcgg ctgtaggcgt
     6181 tgcctgtcat gaggcaggtc atgcagttca gcacgcagaa aattatctgc ctaacagaat
     6241 ccgttcgttt attcttcctg ttgcaagact cggttcaagc ctcagttgga tttttattat
     6301 agtcggtttt tgttttacac agagagtagg ttttgtattt ctttatatag gtataatcct
     6361 gtttgcgctt tccgtactct ttacaatcgc aacacttccc gtagagttta atgcatcaaa
     6421 aagagcgctt gaaacaatca gaaacggcga tcttctttac ggtgaagaat acacaggcgc
     6481 aaagagagtg cttcaggcag ccgcaatgac atatgttgca gcggctctta ctgcaattat
     6541 gcagctttta agattaatta taattgcgaa caatcgcaga agataattta ttttggagat
     6601 taaaatggca aacacacgca atattgcact gaatgtgctg cttaaaattg aaaatgaggg
     6661 agcatattca aatattgctt tgaataatgc cataaaggaa aaccgtcttt cgggtgttga
     6721 cagctctttt gtgtcagcac tcgtttacgg cgttctggaa agaagaatca cgcttgatta
     6781 tattatcagg tcttattcta aaattccgtt aaggaaaatt gaaacaaaaa caaaaaatat
     6841 tcttcgcctt ggaatactcc agcttttgtt tatggacaaa gttcccgaca gtgcggcagt
     6901 caacgaaagc gttaatcttg ccaaaaagca caaacttcag aaatcatcgg gtttcataaa
     6961 cggaattctt cgcaatatca caagggcaga ggataaatat acattacccg atgaaaagga
     7021 cagggctcga tatctttcgg taaaatattc tgttcccgaa aatattgtaa aattgtggat
     7081 taattcttac ggagaaaata atgccgaaca gcttttggca tctcttaacg gcagagccaa
     7141 aatctgttgt cgtgttaata ccctcagaac agatgcagac acattaatta agaaattatc
     7201 cgatgagggc gttgttgcgg aaaaaatccc gtttatagac aacgcactct atcttgaaaa
     7261 tacaggctcg gttgaaagac tcagagcgta caagtccgga ctttttcata ttcaggatgc
     7321 gtcaagccag ctttgtgtga attttcttga cgcaaagcct cgcaatatta tgcttgatgt
     7381 atgttccgca ccgggcggaa agtcgttttc ggcggctcag tatatgaaca accgtggcag
     7441 aattttttcc tgcgatatgt tcgaccataa gctgaaactg atttcggcag gtgctaaaag
     7501 gcttggcatc acttgtattt caacgcttct gcgtgatgcg tcaaccaatg acacggcact
     7561 tcctgtggcg gacagaatcc tctgcgatgt tccgtgcagc ggtcttggaa tcatgtcaag
     7621 aaagcccgag atcagataca aggatgacct tatttccaac gatttgccgg atttgcagta
     7681 caggattctc tgtgcaagtg ctgataatct ggcagtaggc ggtaagcttg tctattctac
     7741 ctgtacattg aatcctgatg aaaacaataa aaacacaaag cgttttcttg aggaacatcc
     7801 cgatttttac ggaataccgt taaaacttcc aaacggaatt acccgtgcat ttgatgaaga
     7861 accatacgaa ataacactta tgccgcacac ctgcggaaca gacggatttt acatcagctt
     7921 attcggaaag aaggcgtcaa attgaataat ttaacggata tcaagtcaat gacgcttgat
     7981 gagctgactg aatttgtcac agaaaacggt tttccaaagt tcagagcaaa gcagatttat
     8041 gattggttgt acaaaaatgt gaccgatttt gacaatatgc gtaacattcc tgccgattta
     8101 aaggcttttt tgaaatcaag tagttacatt tctgttgcaa atattgaaaa aaaacttgtt
     8161 tcaaggtatg ataaaacagt caagtacctt ttttcgttta atgacggcga atgtgtagag
     8221 tctgttgtaa tgagttacaa gcacggatat tcaatctgta tctcaacaca ggttggctgt
     8281 aaaatgggtt gtacattttg tgcaacaggc aaaagcggtt tttcacggag tcttgcaccg
     8341 tcggaaatgc tcggacagat tgaggccgca cagcgtgacc ttaacataag aatttctaac
     8401 attgttctta tgggaatggg tgaaccgctt gataattttg ataatgtcgt aaaatttctc
     8461 cgacttgttt cgtccgacaa cggtcttaac attggaatgc gacatattac gctctcaacc
     8521 tgcggtatag ttccgaaaat ttatgagctt gcaaaacttc atctcggaat aacgctttcc
     8581 gtttcattac acgcgccgaa cgatgagata agacagcgaa caatgcccat tgcaagaaaa
     8641 tattctattg aagaattatt aaaggcttgc tcagattatt tcaaaacaac aggtcgcaga
     8701 gttacatttg aatactctat gataagcgga gttaatgaca gcgatgaaaa cgcaagagag
     8761 cttgcaaaac gacttgaggg tacacaaagc catgttaatc tgatccccgt caatactgtt
     8821 gagggaacag gctacttaaa aagcaatatt aaaagacagc aggcattcat aaatattctt
     8881 gctgctaaaa atatcggagc aaccgtgcgc agaacactcg gaagtgacat aaacgcatcg
     8941 tgcggtcagc tcaaaagaaa gcatattgaa gaaggaggta aataacttgg aagtattcag
     9001 caaaagtgat atcggacttg ttcgtaatca gaatcaggat gactgtcgct tcggagttat
     9061 ttctcccagc tgtgtctggg cagttgtgtg tgacggaatg ggcggtgcaa acggcggtaa
     9121 tattgccagc gcaacggcag ttgattatat cagcaccaaa attaccgatt tgtacaaaga
     9181 tgatatgacc aaggagcaga ttggcgagct tatggcagaa attgttgtca atgccaatat
     9241 gaaagtcttt gaaatgtcca tgaaagatcc cgagctcacg ggtatgggaa ctacatgtga
     9301 atttgtcttt gtaaaagata caaccgttca tgttgttcat gtcggtgaca gtcgcacata
     9361 cgctatcaga ggcggtaaaa tcaagcagct tacagaggat cattccgttg ttcaggaaat
     9421 ggttcgaaga ggcgagctta catatgagca ggcgcaaaat catccgaaca agaactttat
     9481 aacaagggcg ctcggcataa aaccgtccgt tcgccttgac tatattgaag ccaactttat
     9541 ttacggtgat gttcttctca tctgtaccga cggactttca aattgcgtca caacaggaga
     9601 tatggtcaaa atctgccatg aaaaccgtgg cgaaggtctt atcacaaagc ttgttgacaa
     9661 ggcgaaagac ggcggaggtt ctgataatat cacagctacc gtaatttact aaggatagga
     9721 gttgaccgtt ttggataaat atttgggcaa aagacttgac ggaagatatg aaatccacga
     9781 gcttatcggt gtcggcggta tggcaaatgt ttaccgttgt accgacacag tcgatgaccg
     9841 tgaagttgcc gtaaaaattc ttaaagacga gtatctcaac aatgaggagt ttatccgccg
     9901 tttcaaaaat gaatcaaaag ccatcgcaat gctttcacat ccgaatattg taaaggtgta
     9961 tgatgtaagc tttggcgata tgattcagta tatcgtaatg gaatatattg acggcattac
    10021 cctcaaagaa tatattgatc agcagggcat tattgagtgg aaagacgcaa tccacctgac
    10081 cgcacagatt ttaaaggctt tacagcacgc tcacgagtgc ggaattgtac accgtgatat
    10141 aaagccgcaa aatattatgc tgttgcagga cggtacaatc aaagttaccg atttcggtat
    10201 tgcaagattt tccgataaat caacacgcac aatgacagaa caggcaatcg gctctgttca
    10261 ctacattgcc cctgaacagg caagaggtga cgcaactgac ggaaaaacgg acatttattc
    10321 tgtcggtgta atgctctatg aaatgcttac gggaaagctt ccgtttgacg gtgacagtgc
    10381 cgttacaatt gcgcttatgc agttgcagtc aacaccgaag catcctcgtg aaatcaatcc
    10441 gggaatcccg ataggtcttg agcagattac tatgaaggca atggaaaagc ttccgtccga
    10501 cagatacact tctgccgcag aaatgctttc tgatatcgaa agattcagac ttaatccgtc
    10561 aatcgttttt gattacggca acaagtcttt tgttgataat cagcccacaa agtttgttca
    10621 ctctctgcgt gacagcaggg taagaaataa aattgacaac gaggcaacca aagtagttga
    10681 tatgccgcag gacgatgttg ttgttaaaga agaacagttt gccgatgagg attttgtaaa
    10741 ggaacataag ccgtcatact atgcggtcaa gggtattgta attgcggctg ttgcagtatg
    10801 tgcaattttc ttt
//
LOCUS       DAOMVE010000055        10770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1474_length_10770_cov_2.869249, whole genome shotgun sequence.
ACCESSION   DAOMVE010000055 DAOMVE010000000
VERSION     DAOMVE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1474_length_10770_cov_2.869249"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="PLS64_04540"
     CDS             complement(<1..862)
                     /locus_tag="PLS64_04540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL43129.1"
                     /translation="MTEYTFSVFTPSYNRAKTLPRLYEALKRQTYRNFEWIIVDDGSA
                     DNTKETVEKFISDRPDFNITYKYQTNKGKHIATNVAAQIAKGEFFITIDSDDSVKDDA
                     LETFINEWNKIPDEDRSKYKGISCRTCNESGKTNGCPLPSERFDCSDLDLRLKYKIDG
                     EMWGMTKLEIIRANPYPCVSGLHFYPENIYWDNIGRKYITRFIDTPLRYYINDQENSL
                     TGNAYSAHRETIFVRLHFINECWDYAKSNPKFFIKQFVGLSRDGLLNGKRFSEIKKMP
                     DTFSKRALAVI"
     gene            complement(886..2430)
                     /locus_tag="PLS64_04545"
     CDS             complement(886..2430)
                     /locus_tag="PLS64_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL43130.1"
                     /translation="MKKLSTSKQMAINMVAAIVSFLIGTGINFVLAPYLVKELGSETY
                     GFIGLANNFVQYATIVTSALNSMSGRFISIEYSKGNIDKASRYFSSVLVADLIIAGVL
                     LLVSSIITFNIEFILNVPENLVDSVKILFAFTFATFIISVITAIFTTAAFVKNKLYIN
                     SLRDIASNTLKLLLVVILFSFFPAKVYFLAMASLASGIFLLLTNIKVKNKILPEVKMN
                     FNLFSFKLVKVLISAGIWSSIGYLSTALNRGLDLLICNLTLGAGLMGLLSIANTVPHC
                     IDNLITTIANIFTPRFTILYAKDNTDLLVEETKFSSKIMSFLMTAPLAGFIAFGNKFY
                     TLWQPTKSPDEITIIQILSVLVCISYLFTCHTRTLSSLFTVCNKLKASVLISLVCGVL
                     TVIIVLLLIHFTPLGVYAIAGVSSVFIGLKAIVFVPLYAAHILKIKLTTYYRSIFRGW
                     FCFAVVITMFIFINSFVHANSWMSFLLLCAASGLAGYAVSVPLIFSRKELVLIKDKIK
                     KKFKKS"
     gene            complement(2454..3278)
                     /locus_tag="PLS64_04550"
     CDS             complement(2454..3278)
                     /locus_tag="PLS64_04550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRL43131.1"
                     /translation="MVYKIMYPPIPNMGDLLNKDMLEELFNIKVVRKDLKSCNLIAIG
                     SALDHIMYSTYPRIRAKQKIEHFMNDNVHIWSTGFIRGNAELDLGLMFRHIHIHALRG
                     RLSLQRMENILCKKLDVPTGDGGLLAERWLGFYPEKKYLVGIIPHFKEQDHPVVKKLL
                     DNYDNSTLIDLKENPKKVVEKIGECEYIISSSLHGMIVADSFHIPNMHITLTNNMFGD
                     GNKYNDYLSAFDITHTPFDCSNGDMPTISEVKNNYRIDPDVVELKKEQLFNAFPKF"
     gene            complement(3297..4568)
                     /locus_tag="PLS64_04555"
     CDS             complement(3297..4568)
                     /locus_tag="PLS64_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43132.1"
                     /translation="MENTLQNSKDRLFIKLNSFVVAMMISAQTITGTIVRIPAFKDFH
                     NNFIILVIALTIIFAEIIYYKKVTIELPVMFFLSFVTLWYLYSLIFHGNNCDLNVIQF
                     IFYAIIPIYVIGKKLDGELVMRYILYLSLMTLPVINLFFVLQYVKYNQAYMGNIYAIL
                     SPVIVAMIHFKLYRKQSNLLIKLTYIYNLYIMVKIVLFANRGAVLCLACCAIVLVINA
                     YDDDKRKKLSAIKLSLIIVFSVIGILIIIFALPLLHSLADLCNSILNSVPSFISKMIK
                     YLELGDVSDGRTAIDAFTLPAIANNPIFGYGIETFSKVSGELANRSWPYPHQYIYQYL
                     FEGGIVFGLIPVYSSLSLTAKVVCTRIKDKSEFALCCTLVCVTLPKLLISTEPWATTS
                     IWMLITYSLIYICKTNPIFISLNQSFCSRRK"
     gene            complement(4581..5468)
                     /locus_tag="PLS64_04560"
     CDS             complement(4581..5468)
                     /locus_tag="PLS64_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRL43133.1"
                     /translation="MQQLVSVIIPTYKGADKINSAVDSVLNQTYKNIEVIVVDDNGEN
                     EPEQLLTAEKMKKYETFENVKYLVHKKNINGSAARNTGIRASKGFYLGFLDDDDVFLP
                     EKTQKQVDCFEKLPDDYAMIYGSFLEHMTENTDRIVKADNADNFLYRFLCNEIIACSS
                     TIMIKRSVLDYVKEWDESFKRHQDLEFFARVACGCRVACISDVCIEKFKLDRNMPKGE
                     LYEQYRLHYLNKMKPIIEQFTEKEQKRLYNIHYTEIGKAYLKQKQLKKAMHWAFKTSN
                     PAKALFLFVKDGMTFILRK"
     gene            complement(5498..6631)
                     /locus_tag="PLS64_04565"
     CDS             complement(5498..6631)
                     /locus_tag="PLS64_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRL43134.1"
                     /translation="MEPIRVLHNIVLMDAGGIETLVMNLYRHIDKEQILFDFLVHRPQ
                     EGYYEKEIREYGGKVYRTRPFNPLHMREYKKECMNILTSHPEYKVFHVHQELGLWTLD
                     YAHQAGIPTRIAHAHNAKTVVNLKYFFFLYEKMFIKKHCTDMFMCSKPAGEWLFGKQA
                     VQDGKVKYINNSIETDRFKYNEQTRRDMREKLGVGDKIVIGHVGRFAQPKNHTFILDV
                     FNEIHRMNKNTVLVLCGEGKLEDDIKAKTKALGLENDVIFTGIMDTSVLYQALDLFLF
                     PSLWEGLPLTGIEAQTSGLPIVMSDVIADETVVTDNVTRLSLSNSADVWAKTCLDVLD
                     AYERKDCQKQVTDAGFDVCNTAKLLQDFYIDRTLRARHAQKNL"
     gene            complement(6645..7634)
                     /locus_tag="PLS64_04570"
     CDS             complement(6645..7634)
                     /locus_tag="PLS64_04570"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HRL43135.1"
                     /translation="MNVVYSSSDSYSMVAGISMYSLLENNQSVDELNIFLIENGMSQE
                     NKDKFTTMCQKFNRQITFIPISDIEKLTNSSINIGRWNISTFARLFYSSMLPEWVDKV
                     IHIDCDTMIMSSLEPLWNIDMKDKIVAGAYECIGDSYKTEVGMKPDDIYISAGNIMLN
                     LAEIRRRNLEENFKKFIQNHRHLSFVDQPVLNACTTNEDKLLIPLNYNAYSIVFYLNY
                     KNAKKAKRVSRYYTESQVLDAVSNPCIVHFTTCFMDGTRPWIENNNHPMIKQYLDYKQ
                     KSEWADTPLWKDNRSVVKKLSGKMFNILPQGFVAGSIGIVHDKILPALHKIKK"
     gene            complement(7664..8236)
                     /gene="rfbC"
                     /locus_tag="PLS64_04575"
     CDS             complement(7664..8236)
                     /gene="rfbC"
                     /locus_tag="PLS64_04575"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRL43136.1"
                     /translation="MIQKFEFCNTDFEGAYLIKPFCATDIRGAFIKDYSKEVFEANGL
                     EHNLAEVFYTVSHKGVIRALHFQRVHEQAKLVRCIKGKVFDVIVDLRPESPTFKKWQG
                     FYLSEENMNELYVPEHFAHGYLVLEPSVVSYKCGEKFYGEYDDGIMYNDPDLGVEWPF
                     DEIGGIENLIIADKDKNLQSFKKYEEMYLK"
     gene            complement(8261..9181)
                     /locus_tag="PLS64_04580"
     CDS             complement(8261..9181)
                     /locus_tag="PLS64_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL43137.1"
                     /translation="MKKAIVTGSNGFIGSNVCKRLCADGIKVFAVVKDENENIDNIKN
                     LSGIEIVYCELDEIETLADIISDRDIDVFYHFAWVGSAGSLRCDENVQLQNALWTARA
                     LRTADKMQCKKFVNAGSIMEKETYTAVYTQESKPGLPYIYGAGKLIARAICKPIANSL
                     SIDLCWAVITNAYGVGELSPRFVNSTIRKIIANEPLQFTAATQNYDFIYIDDVAKAFG
                     AIGEHGKPNKEYTIGSGNARPLKEFILEMQQTLAPEAKPIFGDVPFTGVNMPLEAFDT
                     ADIETDCYFKPSVSFAEGVSKTMEWLKSLN"
     gene            complement(9213..10259)
                     /gene="rfbG"
                     /locus_tag="PLS64_04585"
     CDS             complement(9213..10259)
                     /gene="rfbG"
                     /locus_tag="PLS64_04585"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HRL43138.1"
                     /translation="MMLDFYKGKKVFITGHTGFKGSWLCKLLANAGADVTGYSLNPPT
                     SPSLFEIANIESDVKSVIGDIRDFDSLKKAFDEAQPEIVLHLAAQPIVRDSYKMPAYT
                     YETNVMGTVNILECVRQSNCVKSFLNVTTDKVYENREWQWGYRENEPLDGFDPYSNSK
                     SCSELVTHSYKNSFFADGRTAISTARAGNVIGGGDFANDRIIPDCVRALEKGEDIIVR
                     NPHSTRPYQHVLEPLYAYLMIAKAQYGDIKFADCYNVGPDECDCITTGELVDLFVKYA
                     GNINRIDKSEKNAVHEANFLKLDCSKLKTVFGWKPHWHVDDAVKKTVEWSCCYMENGN
                     VRDCMDRQIEEFLK"
     gene            complement(10256..>10770)
                     /locus_tag="PLS64_04590"
     CDS             complement(10256..>10770)
                     /locus_tag="PLS64_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004062302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRL43139.1"
                     /translation="RAEKWKVTVVDTGLDTMTGGRLKRISNFIGDKPFFMTYGDGVSD
                     VDINALLNFHKKHGKLATMSAVKPESRFGVLDLTENNEVRAFREKSDIDSGWINAGFM
                     VLEPKVLDYIKDDTVMFEREPMEQLAREGQLMCCKHNGFWQCMDTLRDKERLEKLWND
                     CTAPWKVWSE"
BASE COUNT         3054 a         2241 c         2132 g         3343 t
ORIGIN      
        1 atatgaccgc aagagccctc tttgaaaatg tgtcgggcat cttttttatt tcggaaaatc
       61 ttttgccgtt gagtaagccg tcacgggaaa gtcccacaaa ttgtttgata aagaacttag
      121 gattgctctt tgcgtaatcc caacattcgt ttataaagtg gagtcttacg aaaatagttt
      181 ccctgtgagc cgagtaagcg ttacctgtca aagaattttc ctgatcgttt atgtaatatc
      241 gcaacggtgt gtcaataaaa cgggttatat actttcttcc gatattgtcc caatagatat
      301 tttcgggata aaaatgaagt cccgaaacac acggatatgg gttggctctg attatttcaa
      361 gttttgtcat tccccacatc tcaccgtcaa ttttatactt caatctcagg tcaaggtcgg
      421 aacagtcaaa tcgttctgac ggcagaggac aaccgtttgt tttgccgctt tcgttacagg
      481 tgcggcatga aattcccttg tattttgacc tgtcttcatc gggaatttta ttccattcgt
      541 ttataaatgt ttcaagggca tcgtctttta cgctgtcatc ggagtcaatc gttataaaga
      601 attcaccctt tgcaatctgt gccgcaacat ttgttgcaat gtgttttcct ttgttggtct
      661 gatatttata ggttatgtta aaatcgggcc tgtcgctgat gaatttctca accgtttctt
      721 ttgtgttgtc ggctgaaccg tcatcaacaa ttatccattc aaaatttctg tatgtctgtc
      781 ttttcagcgc ctcatataat ctgggcagag tttttgccct gttatatgac ggtgtaaaaa
      841 cggaaaaagt atattcagtc attgcaacct caaaatgata ataatttagc ttttcttaaa
      901 cttcttctta attttatcct tgataagaac gagttctttt cttgagaaaa tgagtggaac
      961 ggaaacggca tatcctgcca gtccgcttgc tgcacagaga agaaggaatg acatccagct
     1021 gtttgcgtgt acaaagctgt tgataaagat gaacatggtg ataacaacag caaagcagaa
     1081 ccatcctctg aaaatagacc tgtaatatgt tgtaagcttt attttcaaaa tatgtgcggc
     1141 gtataacgga acaaatacta tcgctttaag accgataaaa acagagctga cgcctgcaat
     1201 agcataaaca ccgagcggtg taaagtgtat gagcagtaaa acgataataa ccgtaagaac
     1261 accgcacaca agcgagataa gaacggaggc cttgagcttg ttgcatacgg tgaaaaggct
     1321 agaaagagtt cttgtgtgac aggtaaatag gtatgaaata catacaagca ccgacaaaat
     1381 ctgaattatg gtaatttcgt ccggagattt ggtcggctgc cacaatgtat agaacttgtt
     1441 gccaaacgca ataaatcccg caagaggtgc agtcatgaga aaactcataa tctttgacga
     1501 aaactttgtt tcttcaacaa gtaagtccgt gttgtccttt gcataaagga ttgtaaatct
     1561 gggagtgaat atatttgcaa tggttgtaat aaggttatca atgcagtgcg gaacggtgtt
     1621 tgcaatcgac aaaagtccca tgagtcccgc tccgagagtc aggttacaga ttaacaggtc
     1681 aagacctctg ttcagagctg tgctgagata accgattgac gaccaaattc ctgcggaaat
     1741 cagaactttt accagcttga aactgaaaag gttgaagttc atctttactt ccggcagaat
     1801 tttattcttg actttgatat tggtaagcaa aagaaaaatt ccgcttgcaa gtgacgccat
     1861 agcgagaaaa tatacttttg cgggaaaaaa cgagaacaaa attactacaa gcaagagctt
     1921 gagcgtgttt gacgcaatat cacggaggga attgatatac agcttgtttt ttacaaatgc
     1981 cgcagtagta aatattgccg tgataacgct gattataaat gttgcaaaag tgaacgcaaa
     2041 caatatcttt acgctgtcaa caagattttc gggaacattc agtataaatt caatgttgaa
     2101 tgtaataatt gacgaaacca aaagtaaaac gccggcaata atcaggtcgg caaccagaac
     2161 cgagctgaaa tatcttgatg ccttgtcaat gtttcctttg ctgtattcaa ttgaaataaa
     2221 tctgcccgac attgagttga gagcagatgt tacaatggtg gcatactgga caaagttgtt
     2281 tgcaagtccg ataaatccgt aggtttcaga accgagttcc tttacaagat agggtgcaag
     2341 aacaaagtta attcctgttc ctatcaaaaa agacactatg gcggcaacca tatttattgc
     2401 catttgttta cttgttgata attttttcat aatatgacct tagttttaac tgattaaaat
     2461 tttggaaaag cattgaagag ctgttctttc ttcagttcaa caacatccgg atcgattctg
     2521 taattatttt tcacctcgct gattgtaggc atatcgccgt ttgaacaatc aaagggtgtg
     2581 tgtgtaatgt caaaggcaga gaggtaatca ttgtacttgt ttccgtcacc gaacatattg
     2641 ttcgtaaggg ttatgtgcat attgggaatg tggaaactgt cggcaacaat cataccgtga
     2701 aggctgcttg aaataatgta ttcgcactcg cctatttttt cgacaacctt ttttgggttt
     2761 tccttcaggt caataagcgt tgaattatcg tagttatcaa gaagtttttt cacaacaggg
     2821 tgatcctgtt ccttaaagtg cgggataatt ccgacaagat actttttttc gggataaaat
     2881 ccaagccacc tttcggcaag aagtcctccg tcacctgtcg gcacatcaag ctttttgcag
     2941 agaatatttt ccattctctg caacgaaagt ctgcccctca gagcgtgtat atgtatatgt
     3001 ctgaacatca gccccaaatc aagctcggca ttgcctcgga taaagcccgt actccatatg
     3061 tggacattat cattcataaa atgctcaatt ttctgctttg ctctgatcct cggatatgtt
     3121 gagtacatta tgtggtcaag tgcagaaccg attgcaatca ggttacagct tttaaggtct
     3181 tttcttacaa ccttgatgtt aaacagttct tcaagcatat ctttattaag aagatctccc
     3241 atattcggaa tcgggggata cattatctta tataccattg tattttctcc gttgtattat
     3301 ttgcgccgtg agcagaagct ctgattaaga gatataaata tcggatttgt cttgcatatg
     3361 tatatcagcg aataagtaat aagcatccat atcgatgttg ttgcccatgg ttcggtggaa
     3421 atcagaagtt ttggaagtgt tacgcacaca agcgtacagc acaacgcaaa ttccgactta
     3481 tctttgattc ttgtgcatac aacctttgcc gtcagcgaaa ggcttgaata taccggaata
     3541 agtccgaata caattccgcc ttcaaagagg tattggtaga tgtactggtg cggatacggc
     3601 catgaacggt ttgccagctc acccgaaacc tttgagaagg tttcaatgcc gtagccgaaa
     3661 ataggattgt tcgcaattgc gggcaatgta aatgcatcaa ttgcggttcg tccgtcagat
     3721 acatcaccga gttcaagata tttaatcatt tttgaaatga agctcggaac gctgtttaaa
     3781 atactgttgc acaagtctgc cagcgaatgc aacaggggaa gtgcaaatat aattatcaaa
     3841 attccgatta cagagaagac aataatgagc gacagcttaa tcgcagacaa ttttttgcgt
     3901 ttgtcgtcat cgtatgcgtt tatgacaagc acaatggcac agcaggcaag acagagaaca
     3961 gcgcctctgt ttgcaaacaa aacaatctta accattatgt agaggttgta tatatatgta
     4021 agctttatga gaaggttcga ctgctttctg tacagcttaa aatgtatcat ggcaacgata
     4081 acaggtgata aaattgcgta gatatttccc atatatgcct gattgtattt tacatactgg
     4141 agaacaaaaa acaaatttat aaccggcagg gtcataaggg aaagatagag aatataccgc
     4201 atcacaagtt ccccgtcaag ctttttgcct atgacataaa tcggtattat tgcgtaaaat
     4261 atgaattgaa tcacattcag atcgcagtta tttccgtgaa aaatgaggga ataaagatac
     4321 cacaaggtta caaaagagag aaagaacata acaggaagtt ctatagtcac tttcttgtag
     4381 taaattattt ccgcaaagat tattgtaagt gcaattacca aaattatgaa attgttatga
     4441 aaatctttaa aagccggaat acgaacgatt gtacccgtga tggtctgtgc tgaaatcatc
     4501 atggctacaa caaagctgtt aagttttatg aaaagccggt cttttgagtt ttgaagcgtg
     4561 ttttccataa attacctcgg ttattttcgt agaataaaag tcattccgtc tttaacaaac
     4621 aaaaacagag cctttgccgg attggaagtt ttaaacgccc aatgcatagc ctttttcaac
     4681 tgcttttgtt ttaaatacgc cttgccgatt tccgtataat gaatgttgta caatcttttt
     4741 tgttctttct cggtgaattg ttcaataatc ggcttcattt tattaagata atgaagtctg
     4801 tattgttcat aaagctcgcc cttcggcata ttgcggtcaa gcttgaactt ttcaatgcag
     4861 acatcactta tgcaggcaac cctgcagccg caggcaacac gggcaaaaaa ttcaagatcc
     4921 tgatgacgct taaatgattc gtcccattct tttacatagt caagcacact tcttttaatc
     4981 ataattgtac ttgaacaggc aattatttcg ttgcacaaaa agcggtaaag aaaattgtcg
     5041 gcattatccg cttttacaat acggtcggtg ttctcggtca tatgctccag aaacgagccg
     5101 taaatcattg cgtaatcatc gggaagcttt tcaaagcagt caacctgttt ctgcgttttt
     5161 tccggaagaa atacatcgtc atcatcaaga aagcccaaat aaaatccctt tgatgctctg
     5221 attcccgtgt ttctggcggc agaaccgtta atattttttt tgtgtacaag atattttaca
     5281 ttttcaaatg tttcgtattt tttcattttt tcggcagtaa gcaactgttc cggttcattt
     5341 tcgccgttat cgtcaacaac gattacctca atgtttttgt atgtctggtt aagaacgctg
     5401 tcaacggcag agttgatctt gtcggcaccc ttatatgtag gaatgataac ggaaacgagc
     5461 tgttgcattt ataccacctt aaaatgtttt gctgtaatta taaatttttt tgtgcgtgtc
     5521 ttgctctgag tgtgcggtca atgtagaagt cctgaagaag ctttgccgtg ttgcatacat
     5581 cgaatccggc gtctgtaacc tgtttctgac agtcctttct ttcgtatgcg tcaagcacat
     5641 caaggcaggt ttttgcccat acatcggcag agtttgaaag cgaaagtcgt gttacattgt
     5701 ccgtaacaac agtttcatcg gcaataacat cgctcataac aatcggcaag cccgaagtct
     5761 gggcttcaat gcccgtaagc ggcaagcctt cccataatga cggaaaaagg aaaaggtcaa
     5821 gcgcttgata caatacgctt gtgtccataa tgcccgtaaa aatcacatca ttttcaagac
     5881 cgagtgcctt tgttttagct tttatatcgt cctcaagctt tccttcaccg cagagaacaa
     5941 gaaccgtgtt cttattcatc ctgtgaatct cgttaaagac atcaagaata aatgtatggt
     6001 ttttcggctg tgcaaaacgg cctacatgac cgatgacgat tttgtcgccg acgccgagct
     6061 tttcacgcat atctctgcgt gtctgttcgt tatatttgaa tctgtctgtt tcaatgctgt
     6121 tgttgatata ttttactttt ccgtcctgaa cagcctgttt tccgaacagc cattctccgg
     6181 caggctttga gcacataaac atatccgtac aatgtttttt gatgaacatt ttttcgtaaa
     6241 gaaagaagaa atatttcaga tttacgacag ttttcgcatt gtgagcatgt gcaattcttg
     6301 tcggaattcc tgcctgatga gcataatcaa gcgtccagag tcccagttcc tgatgaacat
     6361 gaaaaacttt gtactccgga tgacttgtga gaatattcat acattccttt ttgtattctc
     6421 tcatatgcag gggattaaac ggacgggttc tgtaaacttt tccgccgtat tctctgattt
     6481 ccttttcgta ataaccctcc tgcggtctgt gaacgagaaa atcaaataaa atctgttcct
     6541 tatcaatatg tctgtataaa ttcattacga gggtttcaat tccgcccgca tccatcagca
     6601 cgatgttgtg caaaactctt atcggttcca tactgacctc cgtatcattt cttgatttta
     6661 tgcagtgcag gaagtatttt gtcatgaaca attccgattg aacccgcaac aaagccctgt
     6721 ggcaaaatat tgaacatctt gccgcttaac ttcttgacga cactgcggtt gtctttccac
     6781 agcggtgtgt ctgcccattc actcttttgc ttgtaatcaa ggtactgttt tatcatagga
     6841 tgattgttgt tttcaatcca cggccttgtt ccgtccataa aacaggtcgt aaaatgaaca
     6901 atacaggggt tggaaactgc atcaagcacc tgcgattccg tataataccg gctgactctc
     6961 ttggcttttt tcgcattttt ataattgaga taaaaaacaa ttgagtaggc gttgtagttg
     7021 agcggaataa gaagcttgtc ctcgtttgta gtgcaggcat tcagtacagg ctggtcaaca
     7081 aaggagagat gacggtgatt ctgtataaac ttcttgaaat tttcttcaag atttcttctg
     7141 cggatttccg caagatttag cataatattg cctgcgctta tatatatgtc gtcgggtttc
     7201 attccgactt ccgttttata gctgtcaccg atacattcat atgcacctgc aacaatcttg
     7261 tctttcatat caatgttcca taacggctca agagaggaca ttatcattgt gtcgcagtca
     7321 atgtggataa ccttgtcaac ccattcgggc aacatacttg aataaaaaag tcttgcaaat
     7381 gtgctaatat tccatcttcc gatgttgatt gatgagtttg tcagcttttc aatatcggaa
     7441 atgggaataa aagtaatctg acggttaaat ttttgacaca ttgttgtgaa tttgtctttg
     7501 ttttcctgcg acataccgtt ttcaatcagg aaaatattta gttcgtcaac agattggttg
     7561 ttttcgagca gggaatacat tgatataccc gcaaccatcg agtagctgtc cgaacttgaa
     7621 taaactacat tcattttacc ctccggggct tatatactaa aacttattta agatacattt
     7681 cctcatattt cttgaacgat tgaagatttt tatctttgtc ggcaataata agattttcaa
     7741 taccgccgat ttcatcaaat ggccattcaa ctcccaaatc cggatcgttg tacataatgc
     7801 cgtcatcata ttcgccgtag aatttttcac cgcatttgta tgaaacaaca gacggttcaa
     7861 gcacaagata gccgtgtgca aaatgttcgg gaacatagag ctcgttcata ttttcttcgg
     7921 aaaggtagaa tccctgccat tttttgaatg taggtgattc gggacggagg tcaacgataa
     7981 catcaaaaac cttgccctta atgcaccgta ccagctttgc ctgttcgtga actctctgaa
     8041 aatgaagcgc acgaattaca cccttgtgag aaaccgtgta gaaaacctcg gcaagattgt
     8101 gttcaaggcc gttagcctca aaaacttcct ttgagtagtc ctttataaat gcacctctga
     8161 tatcggttgc acagaacggt ttgatgaggt atgcaccctc aaagtccgta ttgcaaaatt
     8221 caaatttctg tatcattaaa atacacctta ctttaaaaaa ctaattaagt gatttaagcc
     8281 attccattgt ttttgaaaca ccttcggcaa aagaaaccga gggcttgaaa taacagtctg
     8341 tttcaatatc ggctgtatca aatgcctcaa gcggcatatt tactcccgta aaaggtacat
     8401 cgccgaaaat cggctttgct tcgggggcaa gcgtctgttg catttcaaga ataaattctt
     8461 taagaggtct tgcgtttccg ctgccgattg tatattcctt gttaggttta ccgtgttctc
     8521 cgattgcccc aaatgctttt gcaacatcgt caatatagat gaagtcatag ttctgtgttg
     8581 cggcggtgaa ctgcaacggc tcatttgcaa tgattttgcg gattgtagaa tttacaaaac
     8641 ggggcgaaag ctcaccgacg ccgtatgcgt ttgtgataac tgcccaacag aggtcaattg
     8701 aaaggctgtt tgcaatcggc ttgcaaattg ctcttgcaat cagcttgcct gcaccgtaaa
     8761 tgtacggaag tccgggcttg ctttcctgag tgtaaacagc ggtgtaggtt tccttttcca
     8821 taatagaacc ggcatttaca aactttttgc actgcatttt gtcggctgtt ctgagagcac
     8881 gagcggtcca cagggcgttt tgcaactgaa cattttcatc gcatctgagc gatcctgccg
     8941 agcctaccca tgcaaagtgg tagaaaacat caatatctct gtcggaaatt atgtcagcca
     9001 aggtttcaat ctcatcaagc tcgcaataca caatttcaat gccgctgaga ttctttatgt
     9061 tatcaatgtt ttcgttttca tccttaacaa cggcaaacac ctttataccg tcggcacaaa
     9121 gtcttttgca gacatttgag ccgataaaac cgtttgagcc tgttacaatt gcctttttca
     9181 taagtaaacc tctttttata tttgccgtat gattatttca gaaattcttc aatctgccta
     9241 tccatacagt ctcttacatt gccgttttcc atataacagc atgaccattc gaccgttttt
     9301 ttaactgcat catcaacatg ccaatgaggt ttccagccaa aaacagtctt gagttttgag
     9361 cagtcaagct tgagaaaatt agcctcgtgg actgcattct tttcactttt gtcaatccgg
     9421 tttatattgc cggcatattt tacgaacaag tcaacaagct cgcctgttgt tatgcagtcg
     9481 cactcgtcag gacctacatt ataacagtcg gcaaacttaa tgtccccgta ctgggctttt
     9541 gcaatcataa gatatgcata aagcggttcg agaacatgct gatacggtct tgtggaatgc
     9601 ggattgcgga cgataatatc ctcgcccttt tccaaggctc tcacgcagtc gggaataatt
     9661 ctgtcgtttg caaaatctcc gccgccgata acatttcctg ctctcgctgt ggaaatcgcc
     9721 gttcttccgt cggcgaagaa agaatttttg tagctgtggg ttacaagttc cgagcagctt
     9781 ttgctgtttg aatacggatc aaacccgtca agcggttcgt tttcacgata tccccattgc
     9841 cattccctgt tttcataaac cttgtcggtt gttacattga gaaacgattt cacacaattt
     9901 gactgccgta cacactcaag aatgttgacc gttcccataa catttgtttc atatgtatat
     9961 gcaggcattt tgtagctgtc acgcacaatc ggctgagccg caagatgaag tacaatctca
    10021 ggctgtgcct catcaaatgc ctttttaagc gaatcaaaat cacggatatc tccgataacc
    10081 gactttacat cgctctcgat atttgcaatc tcaaaaagtg acggtgaggt tggcggatta
    10141 agcgaataac ccgttacatc cgcaccggca tttgcaagca gtttgcagag ccaactgccc
    10201 ttgaatcctg tgtgtcccgt aatgaacact tttttaccct tataaaaatc aagcatcatt
    10261 ccgaccacac tttccaagga gcggtacagt cgttccaaag cttttcaaga cgctccttgt
    10321 cacgcaatgt gtccatacac tgccaaaatc cgttatgctt gcagcacata agctgacctt
    10381 ctcttgcaag ctgttccatc ggctcacgct caaacataac tgtatcgtct tttatatagt
    10441 ccaaaacctt cggttcaagc accataaagc ctgcgttaat ccagcctgag tcaatatcac
    10501 ttttttcacg gaaagctctg acttcgttgt tttcggtcaa atcaagcaca ccgaatctgc
    10561 tttcgggttt tacggctgac atagttgcaa gcttgccgtg ctttttgtgg aaattgagca
    10621 gagcgtttat gtcaacatcg gaaacaccgt cgccataggt cataaaaaac ggtttatctc
    10681 cgataaaatt gctaatcctt ttaagcctgc cgcctgtcat cgtgtcaaga cctgtgtcaa
    10741 caacagtaac cttccatttt tcggctcttt
//
LOCUS       DAOMVE010000056        10665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1490_length_10665_cov_3.511593, whole genome shotgun sequence.
ACCESSION   DAOMVE010000056 DAOMVE010000000
VERSION     DAOMVE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10665
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1490_length_10665_cov_3.511593"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLS64_04595"
     CDS             complement(<1..185)
                     /locus_tag="PLS64_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43140.1"
                     /translation="MFKKIAGLFLAAAVMCSSAAITASAAEAEDDAAVAAADQSGEVS
                     ADGSSEVSADASSEVEAG"
     gene            complement(388..3021)
                     /gene="alaS"
                     /locus_tag="PLS64_04600"
     CDS             complement(388..3021)
                     /gene="alaS"
                     /locus_tag="PLS64_04600"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRL43141.1"
                     /translation="MKWTGLNELREKFLEFFESKGCLRLPSFSLVPKDDNSLLLINSG
                     MAPMKKYFTGEVTPPRKRVTTCQKCIRTPDIERVGITARHGTFFEMLGNFSFGDYFKH
                     EATAWAWEFFTKVLEMPPEKLYVSIYEDDDEAFDIWTKEVGVDPSHMVRLGKEDNFWE
                     HGSGPCGPCSEIYFDRGDEKGCGRPDCHVGCECDRFVEVWNLVFTQFESDGKGNYTPL
                     EHPNIDTGMGLERLACVMQGVDNLFLVDTIQNIMKHISQITGVEYGKNEKSDVSLRVI
                     TDHIRSTTFMIGDGVLPSNEGKGYVLRRLLRRAARHGRLLGYNEPFLYKVCDTVIKEN
                     LTAYPELKEKQEFITKVIKVEEESFAKTIDQGFKMLNGIMDSEDVKVLSGEDAFKLND
                     TYGFPIDLTKEILSEKGIKVDVERFRELMKEQKKRSRDARKNAGADAWISDTTDLSNV
                     ANTEFVGYTDLKSTSKVLAIVKDGVRVDSIGEGENAIIVLDKTPFYGEGGGQVGDTGV
                     MESGSFSADVDNTTKNPSGTIYLHACTVKSGSINVGAEVSTAVDEERHADITRNHTAA
                     HLLQSALREVLGNHVQQAGQLVSDDYFRFDFTHFEALTAKELIEVQNLVNKKILEAIP
                     VTTREMPIEEAKKLGAMALFGEKYGDVVRVVSAGDFSVEFCGGTHVKNTANLGLFRIR
                     QEGSVAAGVRRIEALTGMGVLKYINGMSAVIMDVCSLLKIANPKAVVEGVQKAEQLIK
                     EQQREINELNSKLAVAQIGQLFAGTTEENGVRVVTGRIDKANADILRTMCEKARDFAP
                     KCVCVLATENDGKVTFAAACGKEALALGANAGKIIKAVAQKAGGNGGGKPDMAMAGAK
                     QPEKISEALDSVKETVYAMLK"
     gene            complement(3582..4811)
                     /locus_tag="PLS64_04605"
     CDS             complement(3582..4811)
                     /locus_tag="PLS64_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HRL43142.1"
                     /translation="MITNFEKPEILSPAGDMDCLKSALNFGADAVFLAGKMFGMRSAP
                     QNFDNDQLKKACDLAHAKGARIHVTCNTLPRNNEIPHLQEFMENAQACGVDAFIIADL
                     GVFEAAKKYAPKVQRHISTQAGVTNYAAANVLYNMGASRVVLAREIPLDEIAEMRAKV
                     PKELEIECFVHGAMCVSFSGRCLISSYMTGRDANHGDCAQPCRWKYHLYEENRQGQYF
                     PVEETGDGTYLYNSRDLCMIEHIPELVKAGVSSFKIEGRAKSAYYVAVTTNAYRHAVD
                     DYFAEGDDYVLKPWIREELEKISHREYNTGFYFGREPGQVTGNGGYIRHYDNVALCEE
                     LVDDTTAVITQRNKFYVGDTLDVLPPSGVPFEIKCESLTTDEGMPVESAPHPMQRLTM
                     KCNAPVPSGSVIRKKRD"
     gene            complement(4801..6156)
                     /gene="mltG"
                     /locus_tag="PLS64_04610"
     CDS             complement(4801..6156)
                     /gene="mltG"
                     /locus_tag="PLS64_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRL43143.1"
                     /translation="MNPDNFNMSEQELNAQRRRRAENFKLNIDESEYEDDNFVKSEDE
                     PSNLNSYSGQDVKKQMARESKQVLKQKKKEEKKALKAKNKYNRRTFRFIWIISVLIVG
                     AMAAVYMITGMNDLLAVNRTDSSTVKIEIPENPTVDDVTKVLVDNKVIGEPSYFKLFV
                     NVTKSADDFTQGTYEIRRNMDYEAIINFLSSNNNRTDTVSVTITEGESVLEIANTLEK
                     NGALGDRDEFLSLCNSEKFDSDFDFIKAETNADKRYYKLEGYLYPDTYEFYRNENAES
                     VIYKFLNNYETKINEKQTVDGYSKKTTVLKMVEESDTKYSLDQVMTIASIIQAEAADK
                     EDMYYISSILHNRLTADSSLGVSSLGLDSTKFYPYRSLEDVPENDRSTYKSRYDTYDR
                     KGLPYGPICNPGMDAIIAALNPNSSDYYFFCHDSKGQAYYASTLEQQNANLEYIESYD
                     N"
     gene            complement(6163..6765)
                     /locus_tag="PLS64_04615"
     CDS             complement(6163..6765)
                     /locus_tag="PLS64_04615"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRL43144.1"
                     /translation="MPVKNVRELKSQLRAKYKKIRKSCPPEKKQQLDLALQNAFLASE
                     EYKSCDVLFAFVSSPIEVDTSLILETTLKDGKRLALPKCKNNFGEMDFYLVNSLSQLE
                     KGAFSIMEPNPEVCEKYEDFSHGLCLVPGLCFDMQGFRLGFGKGYYDRFLQNFSGTSV
                     GLCYSKCIEHTLPSGVFDRPVDAVITEKYTNRTNNEFVKE"
     gene            complement(6756..7295)
                     /locus_tag="PLS64_04620"
     CDS             complement(6756..7295)
                     /locus_tag="PLS64_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRL43145.1"
                     /translation="MFYVFLAEGFEETEALAPVDVMRRAKLDVKTVGVTGECVTSSHG
                     VPVKADITIDNIDLDDVQGVVLPGGMPGTLNLEANEKVLEAVKYSCENGKIVAAICAA
                     PSILGHLGILDGKKATCFPGFEKELKGADYTGTHTVTDGNIITAKGAGCAIEFGHAIV
                     SLAVSKDAADKVIGDMQCL"
     gene            complement(7309..8097)
                     /locus_tag="PLS64_04625"
     CDS             complement(7309..8097)
                     /locus_tag="PLS64_04625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL43146.1"
                     /translation="MKTIFRKTEKSDTESLKKLWLSCFDEDVTAVNLIFDSDLFDGCC
                     AVVDDRIVSALYLVKGTLNGEKSHYLCGASTDKSFRGNGIMSRLIDFALDDAKNNGDV
                     YSLLFPANDGLYGFYTKLGYALNCTVKSREISRQTLESFAEKGLNIGCSKENSFIYNE
                     NFFEFAKSYYEIYGSKVINKNDCFAVFDENENTADVFYSAYKNINELSALLLENTKSE
                     RFVFTGKSDNALFENCRLQKCGMIKSLDKNHKIPDDVYIGITLS"
     gene            complement(8094..8984)
                     /locus_tag="PLS64_04630"
     CDS             complement(8094..8984)
                     /locus_tag="PLS64_04630"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HRL43147.1"
                     /translation="MLSFKKIEQSDINELKKYYDYDECMGCDTNLLNAYLWRNEYNIK
                     FAFYDDTIVKVYCNPDESVWGYCMPTGKNIKGALEEVFKDAEERNGNLQIVMLTNGNR
                     AMLETMFPDKFDYVRSPENQDYIYTSRDLATLAGKRFHAKRNHISKFYRTYTDTRFET
                     LSDSNIPDALTVAKLWCAENDTDPEASSEIMAIREMCDLKDEYNVKGAVLYVDSKPVA
                     MTLGSEISPKVYDINFEKALREYDGVYAVINNEFAKTLTQYEYINREEDMGLEGLKKS
                     KLSYNPVIILDRWNAIPKKR"
     gene            complement(8971..9645)
                     /locus_tag="PLS64_04635"
     CDS             complement(8971..9645)
                     /locus_tag="PLS64_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRL43148.1"
                     /translation="MKSKIIVIEGLDGSGKATQTKILCEKFSALGKKVTKLEFPNYES
                     PAAAPVKMYLDGEFGDKPDDVNAYAASAFYAVDRVASYLQFWKKECDNGGVILSDRYV
                     TSNIIYQMSKLPKEEWNSFIDWLNDFEYEKLGIPKPDLVIYLDVEPEVSQKLMEKRYG
                     GDNSKKDLHEKNLRFLLDCRKSAVYAAKKCGWKVINCCENGEIKSIEKISEEIERVAA
                     EIYAEL"
     gene            complement(9655..10455)
                     /gene="thyX"
                     /locus_tag="PLS64_04640"
     CDS             complement(9655..10455)
                     /gene="thyX"
                     /locus_tag="PLS64_04640"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRL43149.1"
                     /translation="MKVTLLTHTPNPEQTVAMAAKLCYSPSGIEDIREGLDEEKTSSF
                     IDMLASLGHASPTEHAYFTFGIEGISRACSHQLVRHRIASYSQQSQRYVDGTRFDFVT
                     PPEIAKNEKALAAYEKALKVEADAYKEIRDALAVGYINEKCPDKYSGTDEEIINAYKA
                     DDKKSCNAFIKKANEDARFILPNASTTKIVCTFNARSLQNFFAHRCCNRAQWEIREVA
                     EQMLLQCLEVAPHLFKNCGPSCLFGPCPEGNMCCGKQKEMRQKYGREK"
BASE COUNT         2794 a         2481 c         2179 g         3211 t
ORIGIN      
        1 cctgcttcaa cttctgaaga agcgtcagct gaaacctctg atgaaccgtc ggctgaaacc
       61 tcacctgact gatctgctgc agcaacagca gcgtcatcct ctgcttctgc agcgctagct
      121 gtgatggcag ctgaactgca cattactgct gctgcaagaa aaagacctgc aattttcttg
      181 aacattttta atctcctcct aaaattgttc ataaatatcc aagatagatt ttcatatctt
      241 agtactgctt attatactat aaaaataaca aaactgcaat agttttgatt taaaaccgtt
      301 tttgttaatt gcacaagatt ttatgtaacc gtttggctat tttccacaat tcaaccggca
      361 agtaagccat aaatacacat taaacgatta ttttaacatt gcataaacag tttcttttac
      421 gctgtcaaga gcctctgaaa tcttctccgg ctgtttagca cctgccatag ccatatcggg
      481 ctttccgcct ccgttgcctc cggccttctg agcaacagcc tttatgattt tgcctgcatt
      541 tgcaccgaga gcaagagcct ctttaccgca ggctgcggca aatgtcacct tgccgtcatt
      601 ttcagttgca agaacacata cgcacttcgg ggcaaaatct cttgcctttt cgcacattgt
      661 gcgaagaatg tcggcattag ccttgtcaat tctgcctgtt accactctta caccgttttc
      721 ctccgttgtg cctgcaaaaa gctgaccgat ctgagcaacg gcaagctttg aattaagctc
      781 gttgatttct ctctgctgtt ccttgataag ctgttcagcc ttctgaacac cctcaacaac
      841 tgcctttgga tttgcaattt tgagtaatga gcaaacatcc ataataacag cgctcatacc
      901 gttgatgtac ttgagaacgc ccatacctgt gagagcctca attcttctta cacctgcggc
      961 aaccgaaccc tcctgtctga ttctgaaaag accgaggttt gcggtattct ttacatgtgt
     1021 accgccacag aattcaacag agaagtcgcc tgcgcttaca acacggacaa catcgccgta
     1081 cttttcaccg aagagtgcca ttgcgccgag ctttttagcc tcttcaatag gcatttcccg
     1141 tgtcgtaacg gggattgcct caagaatctt tttatttaca agattctgaa cctcaatgag
     1201 ttcctttgcc gtgagagcct caaagtgagt aaagtcaaaa cggaagtaat cgtctgaaac
     1261 aagctgacct gcctgctgaa catggttgcc gagaacctca cggagagccg actgaagaag
     1321 gtgagccgct gtgtggtttc ttgtgatatc cgcatggcgc tcttcatcaa cagcagttga
     1381 aacctctgca cctacattga tgctgccgct ctttactgta caagcgtgaa ggtaaattgt
     1441 gcctgacgga ttctttgttg tgttgtcaac atcagctgaa aaactgccag actccataac
     1501 acctgtgtcg ccaacctgac cgccgccttc tccgtagaac ggagttttgt cgagaacaat
     1561 gatagcattt tcgccctcac cgatactgtc aactctcaca ccgtccttaa cgattgcaag
     1621 aacctttgaa gttgacttga ggtctgtgta acctacgaat tctgtatttg caacattcga
     1681 aaggtctgtt gtgtcagaaa tccaagcgtc tgcaccggcg ttctttcttg cgtcacggga
     1741 acgctttttc tgctctttca taagctcacg gaaacgctca acatcaacct tgatgccttt
     1801 ttccgaaaga atttcttttg taaggtcaat cgggaaaccg taggtatcgt taagcttaaa
     1861 tgcgtcctca ccgctgagaa ccttaacatc ttcgctgtcc ataatgccgt tgagcatctt
     1921 aaagccctga tcaattgtct tggcaaagct ttcttcctca accttgataa cctttgtgat
     1981 gaactcctgc ttttccttga gttcgggata agctgtgaga ttttctttga taaccgtatc
     2041 gcaaacctta tagaggaaag gctcgttgta accgagaagt ctgccgtgac gagccgccct
     2101 tctgagcaaa cggcgaagta catagccctt gccctcgttt gacggaagaa caccgtcacc
     2161 gatcataaat gttgtactgc ggatatggtc tgtgataaca cggagcgaaa catcgctctt
     2221 ttcatttttg ccgtactcaa cacctgtaat ctgtgagatg tgtttcataa tattctgaat
     2281 tgtgtcaacg aggaaaaggt tgtcaacacc ctgcataaca caggcaagtc tttcaagtcc
     2341 catacctgta tcaatgttcg gatgttcaag aggagtgtag ttacctttgc cgtcactctc
     2401 aaactgagta aatacaaggt tccaaacctc aacaaaacgg tcacattcac agcctacatg
     2461 acagtcgggt ctgccgcagc ccttttcgtc gccacggtca aagtagattt cggagcacgg
     2521 accgcagggg cctgaaccgt gttcccagaa gttatcttcc ttgccgagtc ttaccatatg
     2581 tgacggatca acgccgactt cttttgtcca aatgtcaaaa gcctcgtcat cgtcctcata
     2641 aattgaaaca tagagctttt cgggcggcat ctcaagaacc tttgtaaaga attcccatgc
     2701 ccatgctgta gcctcgtgct taaaatagtc gccgaatgag aagttaccga gcatttcaaa
     2761 gaatgtaccg tgacgggctg tgataccgac acgctcaatg tcgggagtac ggatacattt
     2821 ctgacaggtt gtaacacgct tgcgaggagg tgttacctca cctgtaaaat attttttcat
     2881 aggagccata ccgctgttga taagcagaag gctgttgtcg tcctttggaa caagcgaaaa
     2941 gctggggagt cttaaacaac cctttgattc aaagaattca aggaactttt ctcttaattc
     3001 gttaagtcct gtccacttca taaaaaatct ctccttatac aaattcacag gggaatacaa
     3061 tattcgttca aatttgttta aagtatgcaa ccgagcaaaa taaaaaatcc cattgtataa
     3121 aaatacaatg ggacaggaat atcctgcggt accacccaaa ttgacgccaa gcgtccgctt
     3181 tgagtaatcg tcataagatt acatttcctt aacgcagaaa tacgctgtgc ttaatcacac
     3241 agagctcagg ggcagcgcac cgaaacttca cataaaagcc ttgcaccaaa acggctttct
     3301 ctctgaaatg ctgacatctc ggttaatccc gtcacagccg aatattgtaa ttcaattgta
     3361 gcaaataatt taaatcggag caacactctg ctttgcttgc aaggagcaga gtttgcgaca
     3421 gattgcaatc aagtgcatag gagcgtcagc tcctcagtat ttcagcgaga gatttgctca
     3481 cgctgaaagg ctgaattgtt ccccaccgtt tttaaggtgg ggaacatcag tacttagatt
     3541 ataaaactgt ttctttgtgt tgtcaatagc aaaattcggg attaatcacg cttttttctg
     3601 ataaccgagc ctgacggaac gggagcgttg catttcattg taagtctttg catcggatgc
     3661 ggcgcacttt caacgggcat accctcgtcg gttgtaagtg attcacactt gatttcaaac
     3721 ggtactccgc ttggcggaag tacatcaagt gtgtcgccca catagaattt gttgcgctga
     3781 gtgatcactg cggttgtgtc gtcaacaagc tcttcacaca atgcaacatt gtcataatga
     3841 cggatatatc ctccgttgcc cgtaacctga ccgggttctc tgccgaaata gaagcctgtg
     3901 ttgtactcac ggtggctgat tttttcaagt tcttccctta tccacggttt gagaacataa
     3961 tcgtcaccct ctgcaaaata atcgtcaaca gcgtgacggt aagcgtttgt tgtaacagcg
     4021 acatagtaag ccgactttgc tctgccctca atcttaaagc ttgaaacacc tgcctttaca
     4081 agctcgggga tatgctcaat catacagaga tctcttgaat tgtagagata tgtgccgtca
     4141 cctgtttctt caacaggaaa atactgtccc tgacggtttt cttcgtaaag atgatacttc
     4201 cagcggcacg gctgagcgca gtcgccgtgg ttcgcatctc tgcctgtcat atagcttgaa
     4261 ataaggcatc tgcctgaaaa tgaaacacac attgcaccgt gtacaaagca ttcaatttca
     4321 agctctttcg gaacttttgc acgcatttcg gcaatttcat caagcggaat ttctcttgca
     4381 agcacaaccc ttgacgcacc catattgtaa agcacatttg ccgccgcata gtttgttact
     4441 cccgcctgag tcgaaatgtg gcgctgaacc tttggcgcat actttttagc cgcctcaaaa
     4501 acaccgaggt cggcaattat gaacgcatca actccgcaag cctgagcatt ttccataaat
     4561 tcctgaagat gaggaatttc attattgcgt ggaagtgtgt tgcaggtcac atggattctt
     4621 gcaccctttg cgtgggcaag gtcacaagct tttttgagct gatcgttatc aaaattctgc
     4681 ggtgcggaac gcattccgaa catttttcct gcaagaaaaa ctgcatctgc gccaaagttt
     4741 aaagcggatt taaggcaatc catatcacct gccggtgaca aaatttcggg tttttcaaaa
     4801 ttagttatca tagctttcaa tatactccag atttgcgttt tgctgttcaa gtgttgatgc
     4861 gtaataagcc tgtcccttgc tgtcatgaca gaagaagtaa taatccgagc tgttcggatt
     4921 gagtgcggca attatcgcat ccataccggg gttgcagata ggaccgtaag gaagtccctt
     4981 cctgtcatat gtatcatatc tgctcttgta ggtgcttctg tcattttcgg gaacatcctc
     5041 aagacttctg tacggataga actttgttga atcaagaccg aggcttgaca cgccgagact
     5101 gctgtcggct gtaagacggt tgtgtagaat tgaagaaatg tagtacatat cctctttgtc
     5161 agccgcctct gcctgaatga tagatgcgat tgtcataacc tgatcaagcg aatactttgt
     5221 gtcgctctct tcgaccattt tcaaaactgt tgttttcttt gaatagccgt caacagtctg
     5281 cttttcgtta atctttgttt cgtagttgtt gagaaatttg tagattacgg attctgcatt
     5341 ttcgtttctg tagaattcgt aggtatccgg atagagatag ccctcaagct tgtagtaacg
     5401 cttgtccgca tttgtttcag ccttgataaa gtcaaagtcg ctgtcaaact tttcggaatt
     5461 gcaaagcgaa agaaactcgt ccctgtcgcc caaagcaccg ttcttttcaa gagtgttggc
     5521 aatttcaaga acgctttcgc cctcggtaat tgtaacggaa accgtgtcgg ttctgttgtt
     5581 gttactcgaa aggaagttga tgattgcctc gtagtccata tttctgcgaa tttcatatgt
     5641 accctgagta aaatcatcag cagactttgt gacattgaca aaaagcttga aatatgacgg
     5701 ctcaccgatt actttattgt caacaagtac ctttgtaaca tcgtcaacag tcggattttc
     5761 ggggatttca attttcacgg ttgaggagtc ggttctgttc acggcaagca aatcgttcat
     5821 gcctgttatc atatatacag ccgccattgc tccgacaatt aaaaccgaga taatccagat
     5881 aaagcggaag gttctgcgat tatacttgtt ctttgcttta agagcctttt tttcttcctt
     5941 tttcttctgc tttaaaacct gctttgattc acgggccatt tgctttttga catcctgacc
     6001 gctgtatgaa ttaaggtttg acggttcatc ctcagacttg acgaaattat cgtcctcata
     6061 ctcgctttca tcaatattaa gtttaaagtt ttcggctctt ctgcgacgct gagcgttaag
     6121 ctcctgttcg ctcatattaa aattatcagg gttcataatg tatcattcct ttacaaactc
     6181 gttatttgtt cggttggtat atttttcggt aatcacggcg tcaacggggc ggtcgaacac
     6241 tcccgagggc agagtatgct ctatacattt ggagtagcaa agcccaacgc ttgttcccga
     6301 aaaattctgt aaaaatctgt cgtagtaacc cttgccgaat ccaaggcgga atccctgcat
     6361 atcaaagcaa agtcccggaa caaggcagag tccgtgtgag aaatcctcgt acttttcgca
     6421 aacctcgggg tttggttcca tgattgaaaa cgcacccttt tcaagctgag aaagtgaatt
     6481 tacaaggtaa aaatccattt cgccgaaatt atttttacac tttggcaaag caagcctttt
     6541 gccgtctttc agcgttgttt caagaataag cgaggtgtca acctcaatcg gtgatgaaac
     6601 aaaggcaaaa agcacatcac agcttttgta ttcctcactt gccagaaaag cgttctgcaa
     6661 ggcgaggtca agctgttgct ttttttcggg aggacagctt tttcggattt ttttatattt
     6721 tgcacggagc tgacttttca gctcacgaac atttttcaca ggcattgcat atcaccgata
     6781 accttgtcgg cggcatcctt tgatactgcg agagaaacaa ttgcgtgacc gaattcaatt
     6841 gcacagcctg cgccctttgc ggtaataata ttgccgtccg ttacggtgtg agtgcctgta
     6901 tagtctgcac ccttgagttc cttttcaaag cccggaaaac aggttgcctt tttgccgtca
     6961 aggattccga gatgaccgag aattgacgga gcggcacaga ttgcggcaac gattttgccg
     7021 ttttcacagc tatacttaac agcctcaaga accttttcgt ttgcctcaag attgagagtt
     7081 ccgggcatac cgccggggag tacaacaccc tgaacatcgt caaggtcaat attgtcaatt
     7141 gtgatatcgg cttttacagg cacaccgtgt gagcttgtaa cacattcgcc tgttacaccg
     7201 actgttttaa catcaagctt tgccctgcgc attacatcaa cgggggcaag cgcctctgtt
     7261 tcttcaaagc cttctgcaag aaatacatag aacatagtaa acttcctttc aggaaagagt
     7321 aatcccgata tagacatcgt cgggaatttt atggtttttg tcgagtgatt taatcattcc
     7381 gcatttttgt aacctacagt tttcaaaaag agcgttatcg gatttgcccg taaacacaaa
     7441 cctttctgat tttgtatttt caagcaaaag tgcggaaagt tcgtttatat ttttataagc
     7501 cgagtaaaaa acatcggcag tattttcgtt ttcatcaaat acggcaaagc agtcattctt
     7561 attgattacc tttgagccgt aaatctcata ataggacttt gcaaattcaa aaaaattctc
     7621 attatatatg aaagaatttt cttttgaaca gccgatattc aagcccttct ctgcaaaact
     7681 ttcaagcgtt tgccttgaaa tttcacggct ttttacggtg cagttcaaag catatccgag
     7741 ttttgtgtaa aagccgtaca agccgtcatt tgcaggaaaa agcagagaat agacatcacc
     7801 gttattcttt gcatcatcaa gtgcaaaatc aatcagcctt gacattatcc cgttgccacg
     7861 gaaagattta tcggttgacg caccgcaaag ataatgcgat ttttcgccgt tgagcgtacc
     7921 ttttacaagg taaagagccg aaacgattct atcgtcaaca acggcacagc agccgtcaaa
     7981 aaggtcgctg tcaaaaatca ggttgacggc agttacatcc tcgtcaaaac acgagagcca
     8041 cagctttttt aggctttcgg tatcggattt ttcggttttg cgaaatatcg ttttcatcgt
     8101 tttttcggta ttgcattcca gcggtcgagg ataataacgg gattgtatga aagtttagac
     8161 tttttgagtc cctcaagtcc catatcttcc tcacggttta tatactcata ctgcgtaaga
     8221 gttttggcaa attcgttgtt gattacggca taaacaccgt catattcacg aagtgccttt
     8281 tcaaagttga tgtcatacac cttgggtgaa atttcacttc cgagagtcat ggcaacgggc
     8341 tttgagtcaa catacagtac agcgcccttg acattatatt cgtctttgag gtcgcacatt
     8401 tccctgattg ccataatttc gcttgaagct tcgggatcgg tgtcattttc cgcacaccac
     8461 agttttgcaa ccgttaatgc atcgggaatg tttgaatccg acagagtttc aaaacgggta
     8521 tcggtgtagg ttctgtaaaa ttttgaaatg tggttacgct ttgcgtgaaa ccttttgccc
     8581 gcaagggttg caaggtcacg ggaagtgtag atgtaatcct gattttcggg tgaacggaca
     8641 taatcaaatt tgtcgggaaa cattgtttca agcattgcac gattgccgtt tgtcagcata
     8701 acaatttgca aattgccgtt tctctcctcg gcatctttga aaacctcttc aagcgcaccc
     8761 tttatatttt tacctgtagg catacagtaa ccccagacag actcgtcggg attacaatac
     8821 accttgacaa ttgtatcatc ataaaaagca aatttaatat tgtattcgtt tcgccaaaga
     8881 taagcgttca aaaggttggt atcgcaaccc atgcactcat cataatcgta gtatttttta
     8941 agttcgttta tgtcactttg ctcaattttt ttaaagctca gcataaattt cagccgctac
     9001 cctctctatc tcttccgata ttttttctat actcttaatc tctccgtttt cacagcaatt
     9061 aataaccttc cagccgcatt ttttagcggc ataaactgca ctttttcgac agtcaagaag
     9121 gaaacggaga tttttctcat gcaaatcttt cttgctgttg tcaccgccgt agcgtttttc
     9181 cataagcttc tgcgaaacct cgggttcaac atcaaggtag attacaagat cgggtttcgg
     9241 aatcccgagc ttttcgtatt caaaatcgtt cagccaatca ataaagctgt tccactcctc
     9301 ttttgggagt ttggacatct gataaattat atttgaagtc acatagcggt cgcttaaaat
     9361 cacaccgccg ttgtcacatt cctttttcca aaactgaaga tagcttgcaa cacggtcaac
     9421 cgcataaaat gcactcgccg cataggcatt gacatcatcg ggcttgtcgc cgaattcgcc
     9481 gtcaagatac atcttcacgg gagcggcggc aggactttca taattcggaa attcaagctt
     9541 tgtgaccttt ttgccgagag cggaaaattt ttcgcacaaa atctttgtct gcgtagcttt
     9601 tccgctgccg tcaagtccct caataacaat aattttgctt ttcatatata taccttactt
     9661 ttctctgccg tatttctgac gcatttcctt ttgctttccg caacacatat tgccctcggg
     9721 gcatggtccg aacaggcatg acgggccgca gtttttgaaa agatgcggag caacctcaag
     9781 acattgcaaa agcatctgtt cggcaacctc acgaatttcc cactgcgctc tgttgcagca
     9841 acggtgtgca aaaaagttct gcaagcttct tgcattgaaa gtgcagacaa tttttgttgt
     9901 cgatgcattc ggaagaataa atcttgcgtc ctcattggct ttcttgatga aagcattgca
     9961 ggattttttg tcgtcagcct tgtaggcatt gataatttct tcatccgttc cgctgtactt
    10021 atcgggacat ttctcgttga tgtaaccgac agcaagtgca tctctgattt ccttgtaagc
    10081 gtcagcctca accttgagag ccttttcata ggcggcaaga gccttttcgt tctttgcaat
    10141 ctcgggcgga gttacaaagt caaaccttgt gccgtcaaca tagcgctgtg actgctgact
    10201 gtaggatgca attctgtggc ggacaagctg gtgtgagcag gctcttgaaa tgccctcaat
    10261 gccgaatgta aaatatgcgt gttcggtcgg acttgcatgg ccgaggcttg caagcatatc
    10321 aataaatgag gaagtttttt cctcgtcaag accttctctt atatcttcaa tccctgacgg
    10381 tgaatagcaa agctttgccg ccattgcaac cgtctgttca gggttaggtg tgtgtgtaag
    10441 gagcgttacc tttattgcca tttttagttt tcatctccca ttttcgataa agttattcac
    10501 aatttccgat attcggattt attataacat tattattcag tcttttcaat ggtaaaaaca
    10561 agcaaaaatc ccgacagcac aaaacctgtc gggatttctg attaaaatac tattttcttg
    10621 taattgacca accctttgag ttgaaaccgc ttaccgtttt gcctt
//
LOCUS       DAOMVE010000057        10642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1496_length_10642_cov_3.666194, whole genome shotgun sequence.
ACCESSION   DAOMVE010000057 DAOMVE010000000
VERSION     DAOMVE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10642
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_10642_cov_3.666194"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PLS64_04645"
     CDS             <1..299
                     /locus_tag="PLS64_04645"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRL43150.1"
                     /translation="LGVHDMFDAICGSTLDGSRKDKKDLIPYAVERLGGSLENDRENI
                     VMIGDTWFDTKGARLCGVDFVGVEYGYGDLESMKKEGGRVFVKTTAELLDVLTK"
     gene            384..854
                     /locus_tag="PLS64_04650"
     CDS             384..854
                     /locus_tag="PLS64_04650"
                     /inference="COORDINATES: protein motif:HMM:NF027438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4830 domain-containing protein"
                     /protein_id="HRL43151.1"
                     /translation="MFVFNLKIKAKKTVILAAVLSVTAAIICTAVTVAINSRLPDTAV
                     SDKTGEYSLVLNDGEEKKFLEQFGVETADAKPEKRSVVIPSDFNKYYEEYNELQKKIG
                     LDLGKFKGKEVTEIIVPLEKPKDNNAVLLVYKNRVIGGHITNGEYGSKNLPLVD"
     gene            856..1575
                     /locus_tag="PLS64_04655"
     CDS             856..1575
                     /locus_tag="PLS64_04655"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRL43152.1"
                     /translation="MERLDKIISSQRADGRKEVQKLIKSGAVLVNGKICKKPDTKVDP
                     DKDEIVVDGQALNYSKYIYIMMNKPAGVVSATEDRQFKTVIDIIPEDMKRKGLFPVGR
                     LDRDTEGLLLITDDGDFAHSITSPKKHVDKKYVAKLDGEITEDTVKAFSDGIEFSDGT
                     KCRSAVLECDENDKKTGIVTICEGKYHQVKKMFLCCGLKVVHLQRISIGGLYLDSNLH
                     IGECKLLTDLDKKSVFIGNIH"
     gene            1736..2842
                     /gene="ugpC"
                     /locus_tag="PLS64_04660"
     CDS             1736..2842
                     /gene="ugpC"
                     /locus_tag="PLS64_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRL43153.1"
                     /translation="MANVSLRHVYKIYDGGVTAVTDFNLEIADKEFIILVGPSGCGKS
                     TTLRMIAGLEDISKGELYIGDTLANDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGLK
                     LRKFSKEEIKRRVEEAARILGIEQYLDRKPKALSGGQRQRVALGRAIVRNPKVFLLDE
                     PLSNLDAKLRAQMRTEISKIYQRLGTTFIYVTHDQTEAMTMGTRIVVMKDGFIQQVDT
                     PQHLYDMPCNMFVAGFIGSPQMNFINAVLSKNGGKYTLDFDKYHVPVPESKVNADLDN
                     YVGKEVVLGIRPEHVHDEPEEIAKAECLLKANVDVTELMGAEIYLYVNINGTPITARV
                     EPTSTAKPGDEIEIAFDLSKIHIFDKDTEKTITN"
     gene            3256..4341
                     /locus_tag="PLS64_04665"
     CDS             3256..4341
                     /locus_tag="PLS64_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL43154.1"
                     /translation="MSNRLFQGIVHQMKDAIDRTIGVIDETSVVIACSELGKIGEVNE
                     SVNSETLSSTAPFVVNGYTYRSFGSNAKYDYAVFVQGTDEYAQKYAQLLSVSFASIKQ
                     YYDEKYDRSNFIKNVILDNILPGDIYLKARELHFNSEVSRVCLLIKIVSKTDVSAYDI
                     VQNLFPDKSKDFVININEVEIALVKEIKPDTESRDLEKLASSISDTLSSEFYTHCVVG
                     IGTTVTGIKDLARSFKEAQSALEVAKVFDTDRTIVSYDNLGIARLIYQLPTTLCEMFL
                     KEVFKRGSIESLDQETLFTIQRFFENNLNVSETSRKLFVHRNTLVYRLEKIKKLTGLD
                     LREFEDAIVFKVALMVKKYLNASPTKY"
     gene            4442..5206
                     /gene="ftsE"
                     /locus_tag="PLS64_04670"
     CDS             4442..5206
                     /gene="ftsE"
                     /locus_tag="PLS64_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRL43155.1"
                     /translation="MIDFINVSKTYPNGTHALYGITLSIDKGEFVFIVGASGAGKSTF
                     LKLIMHEETPTSGKIVINDTDVTKLRRSKVPYMRRHMGIVFQDFRLIDKMNVFDNVAF
                     AMRAVGKPSAVIKKRVPYVLELVGLKDKMKNRPSELSGGEQQRVSLARALVNNPEIII
                     ADEPTGNVDPEMSHEIIELLNEINATGTTVIVVTHEHDLVREFDRRVVVLDHGKIKSD
                     TKDLFVKDDYTDDYGDDSGEEENTPDYPDNAVVVPE"
     gene            5263..6141
                     /gene="ftsX"
                     /locus_tag="PLS64_04675"
     CDS             5263..6141
                     /gene="ftsX"
                     /locus_tag="PLS64_04675"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRL43156.1"
                     /translation="MAKEGFKNVWNNRIMSIASVCVLISCLVLTGAAALFSMNVDNVV
                     ESVGKSNETSVYIKDGYSQLEAVYVGKEIEKLDNVESATFLSKEDAIKQYKSTLGDDL
                     FAEMQGRNKLPDSFIVVMKDLSKYDDTVAQIKKIDGVDSISNHRELAKKLTDISNLVN
                     MICIAVVCALTIISIFIIANTIRATMYSRRFEISIMKSVGATNSFVRWPFLIEGMIIG
                     LISAIVSTGAIAILYETAQALVYQIVPIDFIPINNVIWITGGLFAIAGIIIGFFGSFI
                     SIRKYLKMEGNEILGW"
     gene            6174..7490
                     /locus_tag="PLS64_04680"
     CDS             6174..7490
                     /locus_tag="PLS64_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL43157.1"
                     /translation="MNKLKRILCAMLCVCMISIPMAIPTTVSAEDSISDLEQQLQQLE
                     QENQKYQKILDDTKSDIAEKEEYKSALVSKVQVLDEKIAVTREKISSLNDDIKEKQDA
                     YDKGLSEVEDQFDALANRLRILYMSGNATDLEIIFGAKDFSDLIDKMELVKSLANSDK
                     ELISEIQTKLDELSTKKESLEADKKDLETQQASLKSDQDEFNKLISDNDEILKNLYAS
                     NSEAQNSLESAALQSDEIEAKISQYYAAQKAAAEHAAQASQSSSSSGSSSSSSSSSSS
                     GSSSSGSSSSGSSSVIVPSGSGFAWPTPGFVSRSSEWFEDREVYNHGGIDIAGAGIMG
                     TPVVAAADGTVVATNSSCTHNWGKSYSCGCGGGYGNYVMISHAEGKMTVYGHLTSLTV
                     SSGQSVSRGQVIGYVGSTGNSTGPHLHYECRLNGVRYNPMSEYPYM"
     gene            7615..8442
                     /locus_tag="PLS64_04685"
     CDS             7615..8442
                     /locus_tag="PLS64_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43158.1"
                     /translation="MITSLNVVFPEKCGKIRGFFNRLRGDKVCVEIKRARGVSVKQLT
                     YICRRQKVNLNKIDRAIGSQRTRLLCCEEMTFPNDSGYKRFYSPLFSARLCTNMALYA
                     LSKFDTPERLTVGIYDPDAQCTDLVSFVLKYTGNVCIITDNEDVFYDELNTIAEETGA
                     CAVVTHHREQLSNCDLVIAPFEIEENLPVRNDAVILTNGRPKENIKGFVYFRYCFKMP
                     NGFALLRPEGLSEEYFCSALYTLGSQYELGSIVPDLCRNDTEAQTVKSLCSYLARFA"
     gene            8561..9043
                     /gene="greA"
                     /locus_tag="PLS64_04690"
     CDS             8561..9043
                     /gene="greA"
                     /locus_tag="PLS64_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRL43159.1"
                     /translation="MAAKPTMLTAEGLKNLEEELAELQNVKRKEIAEKIKVARSYGDL
                     SENSEYDDAKNEQAIMEARIATIESILKTAQIIDESETSNDKVHLTSVVTIEMVGTDR
                     QMTYKIVGSGSNETNPAQGKISDESPVGKALMDRCVGDVVEVETPGGVKAFKILEISK
                     "
     gene            9132..>10642
                     /gene="lysS"
                     /locus_tag="PLS64_04695"
     CDS             9132..>10642
                     /gene="lysS"
                     /locus_tag="PLS64_04695"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRL43160.1"
                     /translation="MSQKPQQTGTSDVIKVRYEKLDALKEAGRDPFVITTSARDVLTE
                     TIKNNFEEYENKDVCVAGRLLSKRGKGKVSFMDLWDRSGKIQIFAKFDDLGEEEYGFL
                     KKWDIGDIVEVKGFVFKTQMGEISVHAKEVKLLSKSLKPLPEKYHGLTNTDLRYRQRY
                     VDLIMNPEVKDTFVKRSKIIGSIRRYLDNQGFMEVETPMLVANAGGASARPFLTHFNA
                     LDEDLKLRISLELYLKRLIVGGLEKVYEIGRVFRNEGVDTRHNPEFTLMELYQAYTDY
                     NGMMDLTENLYRHVAQEVLGTTTITYNGIEMDLGKPFERITMLDAVKKYSGVDFNEIH
                     SDEEAKAIAKEKGVEFEERHKKGDILNLFFEEFAEEHMIQPTFVMDHPIEISPLTKKK
                     PENPDYVERFEFFMNGWEMANAYSELNDPIDQRERFKAQEALLAQGDDEANTTDEDFL
                     NALEIGMPPTGGIGFGIDRMCMLLTDSAAIRDVLLFPTMKSLDSDKKPSGIPKT"
BASE COUNT         3225 a         2028 c         2407 g         2982 t
ORIGIN      
        1 ttctcggtgt gcatgatatg tttgacgcaa tctgcggttc aacacttgac ggcagtcgaa
       61 aagataaaaa agaccttatt ccgtatgcag ttgaaagact tggcggcagc cttgaaaatg
      121 acagagaaaa tatcgtcatg ataggtgaca cttggtttga cacaaaggga gcaagacttt
      181 gcggtgttga ttttgtcggt gttgaatacg gctacggcga ccttgaaagc atgaaaaaag
      241 agggcggcag ggtgtttgtg aaaaccacgg ctgaactctt ggatgttctt acaaagtaaa
      301 taatttgaaa tcggacaaac ggaattatcc gaagattttt cgcatacatt ttatcagaga
      361 ataaaatgtg cggaggattt ataatgttcg tttttaattt aaaaattaag gctaaaaaaa
      421 cagtaatttt ggcggcggtg ctgtccgtaa cagcggcaat aatatgtaca gccgtcacgg
      481 ttgcaataaa ttcacggttg cccgatacag ccgtaagtga taaaacaggg gagtattctc
      541 ttgtgcttaa tgacggtgag gagaaaaaat tccttgaaca gttcggagtt gaaaccgccg
      601 acgcaaagcc tgagaaaagg tcggttgtaa ttccgtcgga ttttaataaa tattatgagg
      661 aatacaacga actacagaag aaaatcggac ttgacctcgg aaaatttaaa ggcaaagagg
      721 ttacggagat tatcgttccc ctggaaaagc caaaggataa caatgccgtt ttgcttgttt
      781 acaaaaacag ggtaatcggc ggacacatta caaacggaga atacggcagt aaaaatctgc
      841 cgcttgttga ttaagatgga aagactcgat aaaattattt cgtcacaaag agccgacggc
      901 agaaaagagg ttcaaaagct cataaaaagc ggtgcggttc ttgtgaacgg aaaaatctgt
      961 aaaaagcctg atactaaggt tgaccccgac aaggatgaaa ttgttgtgga cggacaggct
     1021 ttgaattaca gcaaatatat atatataatg atgaacaagc ctgcgggagt tgtgtcggca
     1081 accgaggaca ggcagtttaa gactgttatt gatattatcc ccgaagatat gaaacgaaag
     1141 ggactttttc ccgtcggcag acttgaccgt gatacggagg gactgcttct tattacagat
     1201 gacggtgact ttgcgcacag cataacctcg cctaaaaagc atgtggacaa gaagtatgtt
     1261 gcaaagcttg acggagaaat cacagaagat accgtaaagg ctttttctga cggaattgag
     1321 ttctctgacg gtacgaagtg tcgcagtgcc gtgcttgaat gtgacgaaaa tgataaaaaa
     1381 acaggaattg tcacaatttg tgagggtaaa tatcatcagg ttaaaaaaat gtttttgtgt
     1441 tgtggattaa aagttgtgca tcttcaacga atttcaatag gcggtttgta tcttgatagc
     1501 aatttgcaca taggcgagtg caaattgtta acggatttgg ataaaaagtc ggtttttatc
     1561 ggcaatatac actaaaaaat gcatctgaat tttatcctgt gtcggtttta aataaaataa
     1621 tgcatatatc tttggtcaat ttcggtatgg tcaaattgtg cgaaaagtgg tatattattg
     1681 tatgtaataa agctaaggac tttatcgtta tcgactaatt ggaggtttat acacaatggc
     1741 aaatgtttca ttaagacatg tttacaagat ttatgacggc ggtgtaactg ctgttacaga
     1801 cttcaacctt gaaattgccg acaaagaatt catcattctc gttggccctt caggttgcgg
     1861 taaatcaaca accctcagaa tgattgcagg tcttgaggat atttcaaagg gtgagcttta
     1921 catcggcgac acactcgcaa acgatgttgc tcctaaggac agagatatcg caatggtatt
     1981 ccagaactat gctctttatc ctcatatgac tgtatatgat aatatggcat tcggtcttaa
     2041 actccgtaag ttctctaagg aagagattaa gagaagagtt gaagaggctg caagaatcct
     2101 tggtatcgag cagtatcttg acagaaagcc aaaggctctt tcaggcggtc agagacagcg
     2161 tgttgctctc ggtcgtgcta tcgttcgtaa cccgaaggta ttccttcttg acgaaccgct
     2221 ttcaaacctt gatgctaagc tccgtgctca gatgagaaca gagatttcaa aaatttatca
     2281 gcgtctcggc acaacattta tctatgtaac ccatgaccag acagaggcta tgacaatggg
     2341 tacaagaatt gttgttatga aagacggttt cattcagcag gttgatactc ctcagcatct
     2401 ttatgatatg ccttgcaata tgtttgttgc cggatttatc ggttcacctc agatgaactt
     2461 catcaacgct gttctctcta agaacggcgg caagtatact cttgattttg acaagtatca
     2521 cgttcctgtt ccggagtcaa aggtcaatgc tgacctcgac aactatgttg gcaaggaagt
     2581 tgttttgggc atccgtcctg agcatgttca cgatgagcct gaagaaattg ctaaggctga
     2641 gtgtcttctc aaagccaatg ttgatgttac cgagcttatg ggcgctgaaa tttatctcta
     2701 tgtaaatatc aacggtacac ctatcacagc tcgtgttgaa cctacttcaa cagctaagcc
     2761 gggcgacgaa attgagattg ctttcgacct cagcaagatt cacatctttg ataaggatac
     2821 tgagaaaaca atcacaaact gattataatt taatttggtt tttacggcgg agcgttatgc
     2881 tccgccgtct ttgcgtttgc agaaggaaaa cgattgttgc cgtaagcgca ataactttca
     2941 aaacccattt gcgtttaaat tttataattg attaaccgtg atgaatagat atttttcgta
     3001 ttttgtcaca aaaacaacac aatgttgtga tatattaaaa aacaaataag actttgtaac
     3061 aattgtttaa ttttgctttc aaaactgtaa cttgttgctg attatgcaaa ttctctaaaa
     3121 aaattcaaga aaaattgtaa atagaggttg atttttttct cgtttttgtg taatatgtat
     3181 atgagacaga ttgtatttta ttgaactctt ttacaatcac aatatataac tgcggagaaa
     3241 acgaggtaaa aatttatgtc aaacagatta tttcaaggta ttgtacacca gatgaaagac
     3301 gcaatcgaca gaacgattgg cgtaattgac gagacttcgg ttgttattgc ctgctccgag
     3361 cttggaaaaa tcggcgaggt aaacgagagc gttaattccg aaacactcag ctcgacagca
     3421 ccgtttgttg tgaacggcta tacatacaga tctttcggca gcaatgcaaa gtatgattat
     3481 gcagtatttg ttcagggtac cgatgagtat gctcagaagt atgctcagct tttgtcggtt
     3541 tcttttgcaa gcatcaagca gtattatgac gaaaagtatg atcgttcaaa ctttattaag
     3601 aatgttattc ttgacaacat tcttccgggt gacatttacc ttaaagcaag agagcttcac
     3661 tttaacagcg aggtttcccg tgtgtgtctg cttattaaaa ttgtttcaaa aacagatgtt
     3721 tccgcttatg atatagttca gaatcttttc ccggacaagt caaaggactt tgttataaac
     3781 attaacgagg ttgaaattgc acttgttaag gaaattaaac ccgacacaga gagccgtgac
     3841 cttgaaaagc ttgcaagctc gatttcagac acactttcga gcgagttcta cacacactgt
     3901 gttgtcggca tcggtacaac ggttacaggc atcaaggatc ttgcaagaag ctttaaggag
     3961 gctcagtcgg ctcttgaggt tgcaaaggta tttgacacag accgtacaat cgtaagctat
     4021 gataacctcg gcattgcccg tcttatctat cagcttccca caacattgtg tgaaatgttc
     4081 ctcaaagaag ttttcaagcg tggttcaatt gagagccttg atcaggaaac tctctttaca
     4141 atccagcgtt tctttgaaaa caacctcaat gtttccgaaa catcaagaaa gctttttgta
     4201 cacagaaaca ctcttgtgta cagacttgaa aaaattaaaa agctcacagg tcttgacctc
     4261 cgtgagtttg aggatgcaat cgtgttcaag gtagctctta tggttaagaa atacctcaac
     4321 gcaagtccta caaaatactg atttggttaa agtgtgcccg agcacagtgt cttgctgtgc
     4381 tttgggcgtt ttgacttttt tgtgagtatt tgtacaataa ctacaaaaca aaggtgactt
     4441 tatgattgat tttataaatg tgtctaaaac ataccctaat ggcactcatg ctctctacgg
     4501 tattacgctg agtattgata agggcgagtt tgtatttatt gtcggtgcat cgggcgcagg
     4561 caagagtaca tttcttaaac tcattatgca tgaggaaact ccgacatcgg gcaaaatcgt
     4621 tatcaacgac actgatgtta caaagctgag aaggagcaag gttccttata tgcgccgtca
     4681 tatgggcatt gtttttcagg atttcagact tattgacaag atgaatgttt tcgacaatgt
     4741 tgcgtttgcg atgcgtgctg tcggaaaacc ttcggcagta attaaaaagc gtgttccgta
     4801 tgtgcttgag cttgtcggtc ttaaagataa gatgaaaaac agaccgagtg aactctcggg
     4861 cggtgagcaa cagcgtgtaa gccttgcaag agcccttgtg aataaccccg aaattattat
     4921 tgccgatgag ccgacaggta atgttgaccc cgagatgtca cacgaaatca ttgagcttct
     4981 caatgaaatt aacgcaacag gcacaaccgt tattgttgta acgcatgaac atgaccttgt
     5041 tcgtgagttt gacagacgag ttgttgttct tgaccacgga aaaattaaga gcgacaccaa
     5101 ggatttgttt gttaaagatg actatactga cgattacggt gatgacagcg gtgaagaaga
     5161 aaacacaccg gattatcccg ataatgcagt ggttgttccg gaataatgcg tttgcgcaaa
     5221 taggattttg aaaggatttt tgatatgacc ggatttggtt atttggcaaa agaaggtttc
     5281 aaaaatgtgt ggaacaaccg tattatgagt atcgcttctg tctgcgtact tataagctgt
     5341 cttgttctca caggtgcggc ggcacttttt tcgatgaatg ttgacaatgt tgttgaatct
     5401 gtcggcaagt caaacgaaac ctctgtatac attaaagacg gatattcaca gcttgaggct
     5461 gtttatgtag gaaaagaaat tgaaaagctt gacaatgttg agtcggcaac ctttctttca
     5521 aaggaagatg cgataaagca gtataaatct actctcggtg atgatctttt tgccgagatg
     5581 cagggcagaa acaaactccc cgattctttc attgtagtta tgaaggactt gtcgaagtat
     5641 gacgatactg ttgctcagat taaaaaaatt gacggtgttg acagcataag caaccaccgt
     5701 gagcttgcaa agaagcttac ggacatcagc aatcttgtca atatgatttg tattgcggtt
     5761 gtgtgtgctt tgacaatcat ttcaatcttt atcattgcca acacaattcg tgcaacaatg
     5821 tattcacgaa gatttgaaat cagcattatg aaatcggttg gtgcaacgaa ctcgtttgta
     5881 cgatggccgt ttcttattga aggtatgata atcggactta tttccgcaat tgtttcaaca
     5941 ggcgcaattg caatcctcta tgagactgca caggctcttg tttatcagat tgttccgatt
     6001 gatttcattc cgattaacaa tgtcatctgg attacaggcg gcttgtttgc aattgcaggt
     6061 attattatcg gcttcttcgg cagctttatt tcaatcagaa aataccttaa aatggaaggt
     6121 aacgaaatac tcggttggta atcccgacag aaatcttaaa ggaggagatt acaatgaaca
     6181 aattaaaaag aattttatgt gcaatgctct gcgtatgcat gatttcaatt ccaatggcaa
     6241 taccgactac tgtttctgct gaggacagca tctcagacct tgaacaacag cttcagcagc
     6301 ttgaacagga aaatcagaag tatcagaaga ttcttgatga tacaaagtct gacatcgcag
     6361 aaaaggaaga atacaagagc gcccttgtaa gcaaggttca ggttcttgat gaaaaaattg
     6421 cagtaacccg tgagaaaatt tcaagcctta acgatgatat taaggaaaag caggacgctt
     6481 atgacaaggg actttcggaa gttgaggatc agtttgacgc acttgcaaac agactcagaa
     6541 ttctctatat gtcaggcaac gcaaccgacc ttgaaatcat tttcggtgca aaggattttt
     6601 ctgaccttat tgataagatg gagcttgtaa agagccttgc aaacagcgac aaggagctta
     6661 tcagcgaaat ccagacaaag ctggacgagc tcagcaccaa gaaggaatcc cttgaggccg
     6721 acaagaaaga ccttgaaact cagcaggcgt cattgaagtc ggatcaggac gagtttaata
     6781 aactcatttc cgataatgac gagattctta aaaatcttta cgcatcaaat tccgaggcgc
     6841 agaattcctt ggaaagtgcg gctttgcaga gtgatgagat tgaagctaaa atcagccagt
     6901 actatgccgc tcagaaggcg gctgcggaac acgctgctca ggcttcacag agctcaagct
     6961 caagcggttc atcaagcagc tcttcaagca gttcatcaag cggctcaagc agttcgggtt
     7021 catcaagctc aggttcatca tctgtaattg ttccgtcggg ttcgggcttt gcatggccta
     7081 caccgggctt tgtaagccgt tcatctgaat ggtttgagga cagagaagtt tataaccacg
     7141 gcggaattga tattgccggc gcaggcatca tgggaactcc tgttgttgcg gctgccgacg
     7201 gtactgttgt tgcaaccaac agcagttgta cacacaactg gggcaagagc tacagctgtg
     7261 gctgcggcgg cggttacggt aactatgtaa tgatcagcca cgctgagggt aagatgacag
     7321 tttacggtca ccttacaagt cttacagtaa gttcaggtca gtctgtttct agaggacagg
     7381 ttatcggcta tgtcggttca acaggtaact caacaggacc tcacctccac tacgaatgcc
     7441 gtcttaacgg tgtaagatac aacccgatgt ctgagtatcc gtacatgtga taatttcttt
     7501 gatacataca caatttttta gcttgacaca aaatactata aatgatacaa tttgataatt
     7561 aatttaaaag ccttcgcata tacttaaata agtaaatatg cggaggtttt tgttttgatt
     7621 acttcattga atgttgtgtt tcccgaaaag tgcgggaaaa taaggggatt ttttaacagg
     7681 ctgagagggg acaaggtgtg cgttgaaatc aaaagggcaa ggggcgtaag cgtaaaacag
     7741 cttacataca tctgtcgcag acaaaaggtc aatctcaata aaattgacag ggcaatcggc
     7801 agtcagcgca caagactttt gtgttgcgaa gaaatgactt ttccgaacga cagcggttac
     7861 aagcgttttt actcgccgct tttttcagca agactttgta ctaatatggc actttatgcc
     7921 ctcagcaaat ttgatactcc cgaaaggctg acggtcggaa tttatgatcc cgacgctcaa
     7981 tgcaccgacc ttgtttcatt tgtgttgaaa tatacgggca atgtctgcat tatcacagac
     8041 aacgaagatg tgttctatga tgagctgaat accattgccg aagaaacagg tgcgtgtgcc
     8101 gttgttactc accaccgtga acagctttca aattgtgacc ttgtcattgc accgtttgaa
     8161 attgaagaaa atctgcctgt aagaaatgat gcggttatcc tcacaaacgg cagacctaag
     8221 gaaaatataa agggctttgt gtattttcgc tattgcttta aaatgccgaa cggttttgca
     8281 cttttgcggc ctgagggatt gtcggaggaa tacttctgct cagcactcta cacactcggt
     8341 tcacagtacg aactcggttc aattgttccc gatttgtgcc gtaatgatac agaggcgcag
     8401 acagtgaaat ctctttgcag ttatcttgcc cgttttgctt gacaatcggt gtgaaaaaaa
     8461 ttataataaa tctatatgca gttttttgct gatatgaaag aattttatac cgtatgcacg
     8521 cttgtgcagg tgcggtatta actgaaagga acgatataaa atggcagcta aaccaacaat
     8581 gcttactgcg gaaggtctta aaaatcttga agaggaactt gcagaattac aaaatgtcaa
     8641 gcgtaaagaa attgcagaga aaatcaaggt agcccgttcc tacggcgact tgtccgagaa
     8701 cagtgaatat gatgatgcaa agaacgagca ggctattatg gaggcaagaa tcgctactat
     8761 cgagtctatc ctcaaaactg ctcagattat tgatgagtcc gaaacatcta acgataaggt
     8821 acacctcact tctgttgtta caatcgaaat ggttggcaca gacagacaga tgacatacaa
     8881 aatcgttggt tcgggttcta acgaaacaaa tcccgcacag ggcaaaattt ctgacgaatc
     8941 acctgtgggc aaggctctca tggacagatg tgtcggtgat gttgttgagg ttgaaactcc
     9001 cggcggcgta aaggcattta agattcttga aatctcaaag taatttgttt atctattgaa
     9061 tttttgctga cggattctgc ttttgcaatc cgtcagattt attgatgttg aaaagagagg
     9121 aatgattgga aatgagtcag aagccacagc agaccggcac aagcgatgtt atcaaggtca
     9181 gatacgaaaa gcttgacgcc ctcaaagaag caggcagaga tccgtttgta attacaacaa
     9241 gtgcccgtga tgttctcacg gaaacaatta aaaacaattt tgaagagtac gaaaataaag
     9301 atgtatgcgt tgcaggccgt ttgctttcaa aaagaggcaa gggcaaggtt tcgtttatgg
     9361 acctttggga tcgttcggga aaaatccaga tttttgcaaa gtttgacgat ctcggtgaag
     9421 aagaatacgg tttccttaaa aagtgggata tcggcgatat cgttgaagta aagggctttg
     9481 ttttcaaaac gcaaatgggc gaaatttctg ttcatgcaaa agaggttaag ctcctttcca
     9541 aatcactcaa acccctcccc gaaaagtatc acggtcttac aaatacagac cttcgttacc
     9601 gtcagcgtta tgttgacctt attatgaatc ccgaggtaaa ggataccttt gtaaagcgtt
     9661 caaaaattat cggcagtatc cgccgttacc ttgacaatca gggctttatg gaggttgaaa
     9721 ctcctatgct cgttgccaat gccggcggtg cgtcggctcg tccgttcctt actcacttca
     9781 acgctcttga cgaggattta aagctcagaa tttctcttga actttacctc aaaagactta
     9841 tcgtaggcgg acttgaaaag gtttacgaaa tcggcagagt attccgtaac gagggtgttg
     9901 acacccgtca caaccctgag tttactctta tggagcttta tcaggcatat accgactaca
     9961 acggtatgat ggatcttacc gaaaacctct atcgccatgt tgcacaggag gttctcggca
    10021 caacaacaat cacatacaac ggcattgaaa tggatcttgg caagccgttt gagagaatta
    10081 caatgcttga cgctgttaaa aagtattcag gcgttgattt caacgaaatt cattctgacg
    10141 aagaggcaaa ggcaattgct aaggaaaagg gtgttgagtt tgaagaacgc cacaagaagg
    10201 gcgacatcct caaccttttc tttgaagaat ttgcagaaga gcatatgatt cagcctactt
    10261 tcgttatgga tcatccgatt gagatttctc cgcttacaaa gaagaaaccc gaaaatcccg
    10321 attatgttga gagattcgag ttctttatga acggctggga aatggctaat gcatattcag
    10381 agcttaacga tccgattgac cagagagagc gttttaaggc tcaggaggca cttctcgcac
    10441 agggcgatga tgaggctaac acaaccgatg aggatttcct caacgcactt gaaatcggta
    10501 tgcctccgac aggcggtatc ggctttggta tcgacagaat gtgtatgctc ctcacagatt
    10561 cagccgcaat ccgtgatgtt ctcctcttcc cgacaatgaa gagcttggat tccgacaaga
    10621 agccgtcagg cattccgaaa ac
//
LOCUS       DAOMVE010000058        10387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1555_length_10387_cov_3.219028, whole genome shotgun sequence.
ACCESSION   DAOMVE010000058 DAOMVE010000000
VERSION     DAOMVE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10387
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1555_length_10387_cov_3.219028"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="PLS64_04700"
     CDS             complement(<1..388)
                     /locus_tag="PLS64_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRL43161.1"
                     /translation="MKERTYVDDVDRSVYDIKDEENDAYRIKSGLDASIVEKISKEKN
                     DPVWMQNFRLQSLQIYNELKVPNWGPPIDGLNMDDIATYVRPNTRMTAKWSEVPEDIK
                     NTFERLGIPQAERKSLAGVGAQYDSEL"
     gene            complement(402..1163)
                     /gene="sufC"
                     /locus_tag="PLS64_04705"
     CDS             complement(402..1163)
                     /gene="sufC"
                     /locus_tag="PLS64_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRL43162.1"
                     /translation="MADKLLDVNDLTVSVEEKEILHDINLSINKGETHVLMGPNGAGK
                     STLGFALMGSPKYTLNSGEILFNGKNINDETPDKRSKDGIFLSFQSPLEVPGLSLSSF
                     IRNALEKNRGKRIRLWDFKKELRQAMELLQMDPSYSERDLNVGFSGGEKKKAEILQML
                     LLNPKLAILDETDSGLDVDAVRTVSKGVEHYQKNRDGALLIITHSTGILESLKVDYTH
                     IMVNGHIVKTGDASLIDEINEHGFEKYLELYAEKF"
     gene            1347..1754
                     /locus_tag="PLS64_04710"
     CDS             1347..1754
                     /locus_tag="PLS64_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007716594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRL43163.1"
                     /translation="MLISSKGRYTLYVLVDLAKNGQNRYIPLKEIAERQNISKKYMEG
                     ILKTLVDRNLLEGVKGKGGGYKLTKEPKDYSLGEILRLTEGDLASVPCTSKTHEHCNH
                     MESCPSHPIWQQLDNLINNYLDSVTLANLLNNE"
     gene            1886..2482
                     /locus_tag="PLS64_04715"
     CDS             1886..2482
                     /locus_tag="PLS64_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HRL43164.1"
                     /translation="MAFDTFDEGINPGGMRSKNEIKTLICYLYNSVKENIDKGIVIQA
                     ILKQGLANYFETSSAFDDLVTNGNLVPADDEHKTYALTENGVEIAKQLDSILAYSIKE
                     KAYACAVKLLAVKKNEIENKVDIVKSSNGFTVKCSVSGGDVDLMKLDVYAPEMEQALI
                     LKKNFLDNPKKAYKIMLALLTKDKETVGEALEELYGIV"
     gene            complement(2538..2798)
                     /locus_tag="PLS64_04720"
     CDS             complement(2538..2798)
                     /locus_tag="PLS64_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HRL43165.1"
                     /translation="MPWWGICLIIFVTFITFAILHFLGKNKHPFKRSLLSMTLGAATL
                     FAVNLTGAFTGVTLPISLLSLLVSIIGGIPGVTLMLGLNLFF"
     gene            complement(2882..3133)
                     /gene="secG"
                     /locus_tag="PLS64_04725"
     CDS             complement(2882..3133)
                     /gene="secG"
                     /locus_tag="PLS64_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRL43166.1"
                     /translation="MSIWGIVLGVLLAIVSVAIIVVIILQEGNQQGIGAVTGVADSYF
                     SKNKARSIDAFLSRWTKVFAAIFVIFVIALNVLSKFGIL"
     gene            complement(3259..5502)
                     /locus_tag="PLS64_04730"
     CDS             complement(3259..5502)
                     /locus_tag="PLS64_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HRL43167.1"
                     /translation="MAKKSSKTPRRTSPSKTHGVINGAGEVVEQPIVSTIRENYMPYA
                     MSVILSRAIPEIDGFKPSHRKLLYMMYKMGLLNGGRTKSANIVGATMKLNPHGDSAIY
                     DTMVRLSRGYEALLHPYVDSKGNFGKFYSRDMAWAASRYTEAKLDAICNELFRDIDKD
                     TVDFVDNYDNTMKEPSLLPVAFPSVLVNANTGIAVGMASNICSFNLREICETTAALIR
                     DPDHDIKSTLPAPDFTGGCQIIYDENVINQVYETGRGSIKLRAKYVYDKSANCIDILS
                     IPATTTCEVIIEKVIDLVKQGKVKEISDIRDETGIDGLKITIDLKRGIDADKLMTKLY
                     RFTTLEDSYACNFNVLIAGVPRVLGVKALLEEWIAFRIECVRRRTYFDRNKKADKLHL
                     LRGLEKILLDIDKAVKIVRETDEESEVVPNLMIGFGIDEIQAEYVAEIKLRHLNREYI
                     LKRTKDLEDLEKEIAELDEILKSKARIKTIIVKELKSIAEKYGQPRKSIIIYDDVARY
                     EEETVEIPDYPVNLFFTKEGYFKKITPQSLRMSGEQKLKDGDEIIQELEFTNNCDLLF
                     FTDKCQVYKAKADDFAQTKASVLGDYVAAKLGFDEGENAVKMVVTKDYKGMLLFAFEN
                     GKAAKVPLESYATKTNRKKLTGAYSDKSPLVGLLYMPEDEEVLFKASSGNMLLVHTGA
                     LALKTTRSTQGVAVLKPKKGHRLFSIERYKDGTFTNPKRYRTGSLPARGALPVNDDSK
                     DEQLSLI"
     gene            complement(5519..7504)
                     /locus_tag="PLS64_04735"
     CDS             complement(5519..7504)
                     /locus_tag="PLS64_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRL43168.1"
                     /translation="MPRKKTTDYDNTSITTLKGADRVRKRPAVIFGSDGIDGCQHSVF
                     EILSNSIDEAREGYGKKITVTRYSDGSIEVEDHGRGIPVDYNENEKRYNWELVFCELY
                     AGGKYNNNEGESYEFSLGMNGLGLCSTQYSSEYMDVDIRRDGYRYTLRFEKGEIVGKL
                     KKEPYSKKDTGTKIHWKPDLEVFTDIDIQPEYFLDIMKRQAIVNAGIEFVFRNQNGKS
                     FDTTSYNYVNGIVDHVAEVVGENGLSSIQHWETEVKTRDRDDKPEYKCKMDISVAFSN
                     KVHLTEYYHNSSWLEHGGAPDKAVRNAFVYQIDSYLKQNNKYNKTESKIKYDDVEDSL
                     VCVISSFSSVSSYENQTKKAVTNKGIQDAMTAFLRQSLEVYFIENPLEAEKICEQVLI
                     NKRSRENAEKTRLNIKKKLSGNLDITNRVAKFVDCRSKDTEQRELFIVEGDSALGACK
                     QARNPDFQAIMPIRGKILNCLKADYDKIFKSEIITDLLKVLGCGVEVKSKANKNLSSF
                     DMNALRWSKIILCTDADVDGFQIRTLLLTMLYRLTPTLIEEGKVFIAESPLYEITSKN
                     DVYFAYDEVEKDKFIEQLGKEKFTIQRSKGLGENEPDMMNLTTMNPESRRLIKVLPAD
                     AEQTAEIFDVLLGDNLQGRKDYIVEHGNEYIDQLDVS"
     gene            complement(7520..9478)
                     /gene="uvrB"
                     /locus_tag="PLS64_04740"
     CDS             complement(7520..9478)
                     /gene="uvrB"
                     /locus_tag="PLS64_04740"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRL43169.1"
                     /translation="MEFKLHSKYQPTGDQPQTIEKLVNSINAGNREQTLLGVTGSGKT
                     FTMANVIQKVQRPTLVLAHNKTLAAQLCSEFKEFFPENAVEYFVSYYDYYQPEAYVAQ
                     TDTYIEKDSSINDEIDKLRHSATLALSERRDVIIVASVSCIYSLGDPIDYRNMVISLR
                     PGMEKSRDELVKKLVELQYERNDVSFTRNKFRVRGDVVEIFPAASNDSIIRVEFFGDE
                     IDRISEINPLTGELKAVLKHAAIYPASHYIVSGDKMKKALAEIDRELEERLAYFRENG
                     KLLEAQRLEQRTRYDMEMLQEIGFCTGIENYSRIMSGRAPGSSPYTLLSYFPDDFLLF
                     VDESHVTLPQVRGMFAGDYARKKSLIDYGFRLPSALDNRPLNFDEFYSKINQAVYVSA
                     TPGDFELEKSAVVAEQIIRPTGLLDPEISVRPTEGQLDDLVSEINIRTAKNQRTLVTT
                     LTKKMAEDLTAYLEKLGIRVRYMHHDIDTVERMEIVRDLRLGEFDVLVGINLLREGLD
                     IPEVSLVAVLDADKEGFLRSTRSLIQIAGRAARNSEGTVIMYADSVTPSMKVAITETQ
                     RRREIQNKYNVEHGIVPKTIVKKVSDILEISTHDDSEFKNTKKLSKAQKQQLIEKLTK
                     EMKAAAKLLEFEHAAYLRDKIKKLRGEK"
     gene            complement(9640..10347)
                     /locus_tag="PLS64_04745"
     CDS             complement(9640..10347)
                     /locus_tag="PLS64_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HRL43170.1"
                     /translation="MARTTDNEHSGHRERMRKKFIENGFDVFETHEALEMFLYYAIPR
                     KDTNPLAHRLLDRYITVGGVCDAPIDELMKEFGLSENAAALLKMLPEMARLYTESKMS
                     HDNIIDYENLGKIFKAKFIGRTNECVALMLGDAKGKMIYFDIISKGSLNSSDMPVRKI
                     VDLSLRHNAKTAFIAHNHPSGTALPSGSDLDTTIVLKSTLATVGVELIDHFIITDDDY
                     VSLRESRLAGNIFYYEK"
BASE COUNT         2872 a         2232 c         2060 g         3223 t
ORIGIN      
        1 ccagctcaga atcatactgc gctccgacac ctgcaaggga ctttctctct gcctgaggaa
       61 taccgagtct ttcaaatgta ttcttgatat cttcggggac ttccgaccat tttgcggtca
      121 tccttgtgtt cggacggaca taggttgcaa tatcgtccat attaagtccg tcaattggcg
      181 gtccccaatt tggaactttg agttcattat aaatttgaag tgactgcaaa cggaaattct
      241 gcatccacac gggatcgttc ttttcctttg aaattttttc tacgattgag gcatcaagac
      301 ctgatttgat acggtaggca tcattttctt cgtctttgat atcgtagaca cttctgtcaa
      361 catcatcgac atatgttctt tctttcatct taatacctcc gtcagaactt ttctgcataa
      421 agttcaagat acttctcaaa accgtgttca ttgatttcat caatcaacga tgcgtcgcct
      481 gttttaacta tatggccgtt taccataata tgggtataat caactttgag agattcgaga
      541 atacctgtgc tgtgtgtgat gatgagcaat gcaccgtcac ggtttttctg atagtgttca
      601 acaccctttg aaactgtgcg aaccgcatca acatcaagac ccgagtctgt ttcgtcaaga
      661 attgcaagct ttggattgag taaaagcatc tgcaaaattt ctgctttttt cttttcaccg
      721 cccgaaaaac ctacattcag atcacgctcg gaatatgacg gatccatctg caaaagctcc
      781 attgcctgtc tgagctcttt tttaaagtcc caaaggcgga ttcttttgcc acggttcttt
      841 tcaagagcat tacgaataaa tgatgaaagt gaaagccccg gaacttcaag aggactttgg
      901 aatgaaagaa aaattccgtc ctttgacctt ttatcgggtg tttcatcgtt aatgtttttt
      961 ccgttaaaaa gtatttcgcc tgaattaagc gtatatttag gactgcccat aagtgcaaat
     1021 ccaagcgttg acttgcctgc accgttaggt cccataagca catgcgtttc acctttgttt
     1081 atactaagat ttatatcgtg cagaatttcc ttttcctcta cacttacagt caaatcgttt
     1141 acatcaagta atttgtctgc catttcataa tcccaccttg ttatattatc aacccaccaa
     1201 aggagtggat aattaaaata taaccttttc aacccatttt gtcaatgggt tggtattgca
     1261 tttttatcgt tttttgtata taatcaaggt atataagcaa aacaggctta caaaatatgc
     1321 aagccttgcc aaaaggggat aattttatgc tgatttcaag taaaggtcgt tacacgctgt
     1381 atgtgcttgt cgatctggca aaaaacgggc agaacagata tattccgctc aaagaaattg
     1441 cagaaagaca gaatatttcc aaaaaatata tggaaggcat actcaaaacc cttgttgacc
     1501 gcaatttgct tgagggcgta aaaggaaagg gcggcggcta taagctgacc aaagaaccaa
     1561 aagattattc actcggtgaa attctcaggc ttacggaagg cgaccttgcg tctgtcccat
     1621 gcacttcaaa aactcacgaa cattgcaatc atatggaatc gtgtccctca catcctatct
     1681 ggcaacagct tgacaattta atcaataatt accttgacag tgtaactctt gcaaatttgc
     1741 ttaataatga ataaaataaa tacgcacaac ctctttgctt ttgtgaagat aatgtgctat
     1801 aataacaata gacagatata atttaagtta tttttgcaca aattaagctg tcactacgaa
     1861 tctatcagca aaagcaggtg attttatggc tttcgataca tttgacgagg gtattaatcc
     1921 cggtggaatg agaagcaaaa acgaaattaa aaccttgata tgttatttgt ataattctgt
     1981 aaaagaaaat atagacaaag gaattgtcat acaggcaatt ctaaagcagg gacttgccaa
     2041 ttattttgaa acaagttccg cctttgacga ccttgttaca aacggtaacc ttgtacctgc
     2101 cgatgatgaa cacaaaacat atgcgctcac ggaaaacggt gttgaaattg caaaacagct
     2161 tgacagcatt cttgcgtact ctatcaaaga aaaggcatac gcttgtgcgg ttaagctcct
     2221 tgccgtaaag aaaaatgaga ttgaaaacaa ggttgatatt gtaaaaagtt caaacggatt
     2281 tactgttaaa tgttctgtat ccggcggtga tgtggatttg atgaagcttg atgtttatgc
     2341 tcccgaaatg gaacaggctc ttattctcaa aaaaaatttt cttgacaatc cgaaaaaagc
     2401 gtacaaaatt atgcttgcgc tcctgaccaa agacaaagaa accgtcggtg aggctcttga
     2461 ggaactttac ggcattgtat aaaatttcat acaaacaatt aaggggctgt cgatgtgacc
     2521 gccccttctg tgattattca gaaaaaaaga ttaagaccga gcataagcgt tactccgggt
     2581 attcctccta ttattgatac caaaagcgat aaaaggctta tgggcaaagt tacgcccgta
     2641 aaagctcccg tcagattgac cgcaaacaat gttgccgctc ccaaagtcat tgaaagcaga
     2701 gagcgtttga acgggtgctt gtttttgccg agaaagtgca agattgcgaa agttataaat
     2761 gttacaaaaa taatcagaca tattccccac caaggcattt ttaacaactc catataaaaa
     2821 gtaaaaccgc cagaggattg ccctgacggt taattttatg ctgattatta agttaatatg
     2881 cttacagtat accgaacttt gaaagcacat taagtgcaat tacaaaaatt acgaatattg
     2941 cagcaaaaac ttttgtccag cgtgagagga acgcatcaat cgaacgagcc ttattctttg
     3001 agaaataaga atcggcaaca cctgttacag caccgatacc ctgctgatta ccctcctgaa
     3061 ggataattac aacgataatg gcaacagata cgattgcgag caatacgcct aaaacaatgc
     3121 cccaaatgct catttttatc ttcctttcat ttaataaata cgaataaaac aaatcctgct
     3181 ttaataactg ttttataata ccacataaac acagcttttt caagagatat tacaagattt
     3241 ttattaattt tttcaaaatc aaatcagcga aagctgttcg tcttttgaat catcgttcac
     3301 gggcaacgca cctcttgcag gaagtgaacc tgttctgtaa cgctttggat ttgtaaaagt
     3361 accgtcttta tagcgttcta tgctgaaaag tctgtgacct tttttgggct tgagaacggc
     3421 aacaccctgt gttgaacggg ttgttttgag agcgagtgcg cccgtgtgaa caaggagcat
     3481 atttcccgat gacgccttga agagtacctc ttcgtcctca ggcatataga gaagtccgac
     3541 aagcggagat ttgtcggaat atgcgcccgt gagcttttta cggttagtct ttgttgcgta
     3601 ggattcaagc ggaacttttg cggccttgcc gttttcaaaa gcaaagagca gcataccctt
     3661 gtaatctttt gtaacaacca ttttcacagc attttcgcct tcatcaaagc caagctttgc
     3721 ggcaacatag tcaccgagta cgcttgcctt tgtctgtgca aaatcatcag cctttgcctt
     3781 atacacctga cacttgtctg taaagaacaa aaggtcgcag ttgtttgtga actcaagctc
     3841 ctgaataatt tcgtcaccgt ctttgagctt ttgttcaccg ctcattctga gcgactgcgg
     3901 agtaatcttc ttgaaatatc cctcttttgt aaagaaaaga ttaacaggat aatcgggaat
     3961 ctcgactgtc tcttcctcat accttgcaac atcgtcatag attatgatac tctttctcgg
     4021 ctgaccgtat ttctcggcaa ttgatttgag ttctttgaca atgattgtct tgattcttgc
     4081 cttgcttttt aaaatttcgt caagctcggc aatctctttt tcaaggtctt caaggtcttt
     4141 tgtacgcttt aaaatgtatt cacggttaag atgacgaagt tttatttcgg caacatattc
     4201 agcctgtatt tcgtcaattc cgaatcctat cataaggttt ggcacaacct cggattcttc
     4261 atcggtttca cggacaatct ttactgcctt gtcaatatcg agaagaattt tttcaagacc
     4321 tcttaaaaga tgaagcttgt cagctttttt gttgcggtca aaatatgttc ttcttctgac
     4381 gcactcaatt ctgaaagcaa tccattcttc aagcaacgcc tttacaccga gaactctcgg
     4441 aacaccagca ataagcacat tgaagttgca ggcataggaa tcttcgagtg ttgtaaagcg
     4501 gtaaagcttt gtcataagct tgtcggcatc aatgccacgc ttgaggtcaa ttgtaatttt
     4561 aagtccgtca atacctgttt cgtcacgaat atcggaaatt tccttgactt taccctgctt
     4621 tacaaggtcg attacctttt caatgataac ctcacaagtt gttgtggcag gaattgaaag
     4681 aatatctata cagtttgcag acttgtcata gacatacttt gcacggagct tgatacttcc
     4741 tctgccggtt tcatagactt gattgatgac attttcgtca tagataatct gacaaccgcc
     4801 tgtaaaatca ggagccggca gagttgactt tatatcatgg tcgggatcac ggataagtgc
     4861 cgctgttgtt tcgcagattt cacgcagatt gaatgaacag atatttgacg ccataccaac
     4921 ggcaatacct gtgtttgcgt ttacaagtac agacggaaat gcaaccggca aaagtgacgg
     4981 ttctttcatt gtattatcgt agttatctac aaaatctacg gtatctttgt ctatatcacg
     5041 aaacagctcg ttgcagattg cgtcaagctt tgcctcagta taacgggatg ccgcccacgc
     5101 catgtcacgg ctgtagaatt taccgaagtt accctttgaa tctacatacg gatgcaaaag
     5161 tgcctcatag ccccttgaaa gacgaaccat tgtgtcataa attgcagagt cgccgtgagg
     5221 attgagcttc attgttgcac cgacaatgtt tgccgatttt gttcttccgc cgttcagaag
     5281 tcccattttg tacatcatat acaggagctt tctgtgcgac ggcttgaatc cgtcgatttc
     5341 gggaattgca cgggagagaa ttacgctcat tgcatacggc atatagttct ctctgatagt
     5401 ggaaacgatt ggctgttcca ctacttcacc cgcaccgttg attacaccgt gagtttttga
     5461 cggtgaagtt ctgcggggag tctttgaaga ttttttcgcc atttatttcc acctccaatc
     5521 agcttacatc aagctggtca atatattcgt tgccgtgttc tactatataa tcttttcttc
     5581 cctgaagatt gtcaccgaga agaacatcaa aaatttccgc tgtctgttct gcatcggcag
     5641 gaagaacctt gataagtctg cgtgactcgg ggttcattgt ggtaaggttc atcatatcgg
     5701 gttcgttctc accgagacct tttgaacgct gaattgtgaa tttctccttg ccgagctgtt
     5761 caatgaactt atccttttca acttcatcgt atgcaaaata tacatcgttt ttggatgtaa
     5821 tttcataaag aggcgattcg gcaataaaca ccttgccttc ttcaatgagt gtaggtgtaa
     5881 gtctgtaaag catggtaagt aaaagtgttc tgatctggaa gccgtcaaca tcggcatcgg
     5941 tgcagagaat aattttgctc cagcgaagtg cgttcatatc aaagcttgaa agattcttgt
     6001 ttgcttttga cttaacctca acaccgcaac cgagaacctt gagcaaatcg gtaatgattt
     6061 cgctcttaaa aatcttatcg tagtcggctt tcaggcagtt aagtatttta cctctgattg
     6121 gcataattgc ctgaaaatcg gggtttcttg cctgtttaca ggcaccgaga gcagaatcac
     6181 cctctactat gaacagttca cgctgttccg tgtctttact tctgcaatcg acaaattttg
     6241 caacacggtt tgttatgtcg agattgcccg aaagcttttt ctttatgtta agtcgtgttt
     6301 tctcagcatt ttcacggctg cgcttattga taagcacctg ttcgcagatt ttttctgcct
     6361 caagcggatt ttcaataaaa tagacttcaa gactctgacg gagaaatgca gtcatagcgt
     6421 cctgaatacc cttgtttgta accgcctttt tggtctggtt ttcatatgat gaaacacttg
     6481 aaaaagacga aataacgcac acaaggctgt cctcaacatc gtcatacttg attttgcttt
     6541 cggttttgtt atatttatta ttttgtttta agtagctgtc aatctggtaa acaaaggcat
     6601 tccgcaccgc cttatcggga gcgccgccgt gttcaagcca gcttgagttg tggtagtatt
     6661 cggtgagatg cactttattt gaaaatgcaa ccgagatatc cattttgcac ttatattcgg
     6721 gcttatcgtc acggtcacgg gttttaacct cggtttccca atgctgaatt gagctgagtc
     6781 cgttttcacc gactacctcg gcaacatggt caacaatacc gtttacatag ttatagcttg
     6841 tcgtatcaaa gcttttgccg ttctgattgc ggaaaacaaa ttcaattcct gcgttgacaa
     6901 tagcctgtct tttcattatg tcaagaaaat attcgggctg aatgtcgata tccgtaaaga
     6961 cttcgaggtc gggtttccaa tgaatttttg tacccgtatc ctttttgctg tacggctctt
     7021 ttttgagctt gccgacaatc tcgccttttt caaaacggag agtgtatcgg tagccgtcac
     7081 ggcggatatc aacatccata tactcggagc tatactgggt tgaacaaagg ccgagaccgt
     7141 tcataccaag ggaaaattcg tagctttcac cctcgttgtt attatactta ccgccggcat
     7201 agagttcgca gaaaaccagt tcccagttgt agcgcttttc gttttcgttg tagtcaacgg
     7261 gaattcctct gccgtgatct tcaacctcaa ttgagccgtc ggaatagcgt gtaactgtga
     7321 tttttttacc gtaaccctct ctcgcctcat ctattgagtt tgaaagaatc tcaaacacgg
     7381 agtgctgaca gccgtcaata ccgtccgagc cgaaaattac ggcaggacgc ttacggacac
     7441 ggtcggcacc ctttagcgtt gtgatactgg tattatcata atcggtggtt ttctttcttg
     7501 gcattttact cttcctttat catttttcac cacgcagttt tttgattttg tcacgcagat
     7561 atgcggcgtg ttcaaattca agcaactttg cggcagcttt catttccttt gtcagttttt
     7621 ctatcaactg ttgtttttgt gcctttgaaa gcttttttgt atttttaaat tcactgtcat
     7681 cgtgagtaga aatttcaagt atatcgctga cctttttgac aatagtcttc ggaacgatgc
     7741 cgtgttcaac attatattta ttctgaattt cacggcggcg ctgtgtttcg gttattgcaa
     7801 ccttcattga cggtgttacg ctgtcggcat acataataac cgtaccctcg ctgtttcgtg
     7861 cggctctgcc ggcaatctga ataagcgaac gggttgaacg gagaaatccc tctttgtcgg
     7921 cgtcaagtac ggcaacaaga gaaacctcgg gaatatcaag tccttcacgc agaaggttga
     7981 ttccgacaag aacatcaaat tctccgagtc ttaaatcacg gacaatctcc attctttcaa
     8041 cggtgtcaat gtcatggtgc atataacgaa ccctgatacc cagcttttca agatatgcgg
     8101 taaggtcttc cgccattttc ttggtaaggg ttgtgacaag tgttctttga tttttggctg
     8161 tacggatatt gatttcggac acaaggtcgt caagctgacc ttcggtaggt cttaccgaaa
     8221 tttcaggatc aagaagtcct gtaggtctga taatctgctc tgcaacaaca gccgattttt
     8281 caagctcaaa atcgcccggt gttgcgctta cataaacagc ctgatttatt tttgaataga
     8341 attcgtcaaa gttgagcgga cggttatcaa gtgcagacgg aagtctgaaa ccgtagtcaa
     8401 taaggctctt ttttcttgca tagtcacccg caaacattcc ccgtacctgt ggaagcgtta
     8461 catgggactc atcgacaaag agcagaaaat catcggggaa atagcttaaa agcgtgtacg
     8521 gcgatgagcc cggcgctctg cccgacatta ttcttgaata gttctcgata cccgtacaaa
     8581 atccgatttc ctgcaacatt tccatatcgt accttgtacg ctgttcaagt cgttgtgcct
     8641 caaggagctt tccgttttca cgaaaatatg caagtctttc ctcaagctcc ctgtcaattt
     8701 ccgcaagagc ctttttcatc ttatctcccg acacaatgta gtgtgacgca ggatagattg
     8761 cggcatgttt cagaacggct ttgagttcgc ccgtaagcgg attgatttcg gaaatcctgt
     8821 caatttcatc tccgaaaaat tcgacacgga ttatagaatc gtttgatgcg gcagggaaaa
     8881 tttcaaccac atcaccacga accctgaatt tattacgggt aaagcttaca tcattgcgtt
     8941 catactgcag ttcaacaagc ttttttacaa gctcatcacg ggacttttcc attcccggac
     9001 gaagtgagat taccatatta cggtagtcaa taggatcgcc caggctgtat atgcaagaaa
     9061 ccgaggcaac aataattaca tctcgtcttt cggaaagtgc aagcgttgcg ctgtggcgga
     9121 gtttatcaat ctcatcattg attgaactgt ctttttcaat ataagtatcc gtctgtgcaa
     9181 catatgcctc aggctgatag taatcgtagt atgacacaaa atattcaacc gcattttccg
     9241 gaaagaactc tttgaactca ctgcaaagct gagccgcaag agttttgttg tgtgcaagca
     9301 caagtgtcgg tctttgaacc ttctgaataa catttgccat tgtaaaagtt ttacccgaac
     9361 ccgttacacc gaggagcgtc tgttcacggt tgcctgcatt tatactgttt acaagctttt
     9421 cgattgtctg cggctggtcg cctgtcggct gatattttga atgaagttta aattccataa
     9481 tacacatcac attccaaata cggatttttt attatgtttc ctgttacaag gctctttatt
     9541 acaaaagtcc tgtaacagaa aacatatccg aacaaaattt cgattttaat tatagaacag
     9601 atgttttaaa ttgtcaaatg tttcttttaa agaacattat tacttttcat aatagaagat
     9661 gttccccgca agcctgcttt cacggagaga aacataatca tcatcggtta ttatgaaatg
     9721 gtcgataagc tcaactccga cagttgcaag cgtactcttc aaaactattg ttgtgtcaag
     9781 gtcacttccc gaaggcagag ctgttccgct gggatgatta tgtgctataa acgcagtttt
     9841 ggcattgtga cgaagtgaca aatcaacaat ttttctgacg ggcatatcgg atgaattgag
     9901 tgaacctttt gaaattatat caaaataaat catcttgccc tttgcatcac cgagcatgag
     9961 agcaacacat tcgtttgttc gaccgataaa ttttgcttta aatattttac cgagattttc
    10021 atagtcaatg atattatcat gtgacatctt tgattcggta taaagccttg ccatttcggg
    10081 caacattttg agcaatgcgg cggcattttc agacagaccg aattccttca taagctcgtc
    10141 aatcggagcg tcacacactc cgccgacagt gatataacgg tcaagcagac gatgtgcaag
    10201 aggatttgtg tcctttctcg gaatagcata gtaaaggaac atttcaagag cctcgtgcgt
    10261 ttcaaacaca tcaaagccgt tttctatgaa ctttttacgc attctttcac gatgaccgct
    10321 gtgttcattg tcagttgtcc gtgccatatt atccccaccg caaaataagt tttttcaaac
    10381 tttgggg
//
LOCUS       DAOMVE010000059        10321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1575_length_10321_cov_3.458017, whole genome shotgun sequence.
ACCESSION   DAOMVE010000059 DAOMVE010000000
VERSION     DAOMVE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1575_length_10321_cov_3.458017"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3338)
                     /gene="pulA"
                     /locus_tag="PLS64_04750"
     CDS             complement(<1..3338)
                     /gene="pulA"
                     /locus_tag="PLS64_04750"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HRL43171.1"
                     /translation="MRLRKPLSILLSLSMIAGMSAFASNAAVTSDESVSAGNYYNANY
                     LESYASKAYNESGLGSVYSKTSTTWKTWSPDASSVKLKLYTTGSDNEAGASAIGTYDM
                     KKDSSTGVWSLNLSGDYKNKYYTYLVTVNGTTKETQDVYSQAVGVNGNRTMVVDLDST
                     DPSGWSDDKHVLFNSASEAAVWEVHVRDFSVSKNSGVSEDNKGKYLAFAEGGTTLNSD
                     TSSSAVSTGIDYLVEQGINCVQLMPVYDYGSVKEDVASSSSNRNWGYDPVNYNAPEGS
                     YSTNPYDGNTRITEFKQMIQALHDRGISVVMDVVYNHTFSTDSCFNRTVPGYYYRMHS
                     SSAYSNGSGCGNETASDKLMYRKYMIESVKYWAEEYHIDGFRFDLMGIHDITTMNDIR
                     SALDGLYSDGSGKKILMYGEPWTGGSVAISDGCSQSKAGSLNSRVGMFCDSYRDAIKG
                     STDGSDKGFVQGNTDKAGTVANGVTGKGFSAQAPSQTIAYADAHDNLILWDKIVKSNG
                     SSSWNSTSSSLRGQVKKVMGLLLTSQGIPFMTAGSEFCRTKQGDTNSYKSSDAINEID
                     WSRVKTYSDVAAYYKGLLEIRENYSPMKSSTFNTPSFQSTHGDVVAYTYSNNKSNEWG
                     KVCVLVNASSTNDWPITLDGSGWTVVADGTTAGLKSLGTVSGNTYTVPANSACVLVQS
                     STFNNLKVSEKTFGTVTIKHIDDSGNVLKTSTAKYADGTTYRTYPDTTILYDYTLKDT
                     QGVTSGTVTGGKNYNVTYVYSSSGIRSGYVTVNYVDENGESIKDTVSTKYREGDSYSV
                     PFTSIQGYQLDTDKYPANTTGTFNGTNTTINFVYKALDSTSSVVHYYNSNNWSNVRCY
                     AYTDGGEEPNGKWNNATVMTSEGNGWLKCTIPAPSSYVMFHTNSQQEPGANETGYLVS
                     GEAWVQNKKLSFSSKVITSHIDAATGEKIADDEILIQSKVSSDDTYKTSPLSGRTDVI
                     APVNASGNLSSGIINVVYLYTSSERPSTAPSTVTPTTAPVTQPTEKILIGDVNLNGAI
                     NVLDATAVQKYIVKLITLSDKALIAAARCDADGENDIVSVKDATYIQMYVAKLDGHGN
                     VGTYYEPEVTPTTAPVTEPATEEPT"
     gene            3827..4042
                     /locus_tag="PLS64_04755"
     CDS             3827..4042
                     /locus_tag="PLS64_04755"
                     /inference="COORDINATES: protein motif:HMM:NF039477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6514 family protein"
                     /protein_id="HRL43172.1"
                     /translation="MITYKLNHTPENGFNTFGISAFCGKKLIKSVSGISESFREMTEL
                     VEMCNELELELCQLDDVIEDYLTDFTL"
     gene            4133..5011
                     /locus_tag="PLS64_04760"
     CDS             4133..5011
                     /locus_tag="PLS64_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRL43173.1"
                     /translation="MICNLCPRKCGAVRTAHSGNGFCKMGELPVVARVAPHYGEEPCI
                     SGTKGSGTVFFSGCTMRCVYCQNYEISNEDGGRTITPERLAECYKELEDKGVHNINLV
                     TADHFAHAVVKSLEIYKPRIPIVYNCSGYTSPKTLSMLDGLVDIYLPDFKYADDFLAV
                     KYSSAPNYVNTAVAAIHEMTFQVGLPQFDDDGIMKKGVIVRHLILPAHTKNSLEVLDI
                     VKRRFGDQVLLSLMCQYIPMGKVSDREFSRINRKITRREYDKVKLEMMRLGIDGFTQE
                     LSSADESYVPEWNFEG"
     gene            complement(5320..7059)
                     /locus_tag="PLS64_04765"
     CDS             complement(5320..7059)
                     /locus_tag="PLS64_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HRL43174.1"
                     /translation="MALDGIFLRHIKSEIEKEALGARVSQIYQPNREELVFGLRTFSG
                     NKKLLLSARANSPRVNFCSSTPENPAQPPMFCMLLRKRIGGGKLVGLRQNGCDRVLML
                     DFECVNELGDTVMITVVCEIMGMYSNIIIVDSNGVIIDSLKRVDLTMSSKRLVLPNIK
                     YELPESQNKLNLLKCSVLDVCTAVKNLDTEMPLNKALLRTIEGVSPIVCREIEYKVME
                     GATNRIEGVLFDRLAVEIEKLKSVCSDCSGKPVVVYREDGKPMDFCFMNVEQYGDFAK
                     VESKESFSDLLDGFYEARDSRDRMRVKSQSLTKLLNNTLERLARKIAKQKSELERCAD
                     REHLRICGDLLQANIYRIERGAEYVDVENFYDENNAILRIKLNPAISPSANAQKYYKD
                     YRKAKNAEIMLKEQLEKGREELDYINSVLDTVDRAENEKDLAQIREELTEQGYLKVQK
                     GKKPRQTTLPPLEFESSDGFKILVGRNNRQNDKLTLKTASKNDMWLHTKDIHGSHVIV
                     VSDGKEISDTAIYEAARLAAYHSKARNSSQVPVDCTLVRYVSKPNGSKPGMVIYVNNK
                     TLYVKPSQTVEKQ"
     gene            complement(7074..7559)
                     /gene="rlmH"
                     /locus_tag="PLS64_04770"
     CDS             complement(7074..7559)
                     /gene="rlmH"
                     /locus_tag="PLS64_04770"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRL43175.1"
                     /translation="MIKINIICIGKIKEKYFTDAIAEYAKRLTAFCKFNIIELSEERI
                     KSNIPNRSQIDEVIFSEGKRIMQKISPSDYVVAMCIEGKSMSSEELSKLIDNASVSGK
                     STVDFIIGGSYGLDESVKKRADVRLSMSRMTFPHQMARMILSEQIYRAFEISSNGKYH
                     K"
     gene            complement(7581..8369)
                     /locus_tag="PLS64_04775"
     CDS             complement(7581..8369)
                     /locus_tag="PLS64_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRL43176.1"
                     /translation="MARAVPLFSGSKGNSYYIGSASEGVLIDAGRSCKQIENAMLANG
                     LDMGSIGAVFITHEHTDHCSALRVLAKRYGFKVYSSLGTLNALRAGNKLDPNTDADVI
                     TDEVVIGNMRVQRIDTPHDAAESCCYRVTAPDGKSALIATDMGVMLPDVRTAAEQSDF
                     VVLESNHDINMLKNGFYPYPLKQRILSNRGHLSNEACAKELVNLVEKGTLRLMLGHLS
                     EENNTVPLAMSTSVDLLTSNGMKYKSDYSLDVAPSVSTTKAIIF"
     gene            complement(8492..9784)
                     /locus_tag="PLS64_04780"
     CDS             complement(8492..9784)
                     /locus_tag="PLS64_04780"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRL43177.1"
                     /translation="MEKFVVTGGKPLSGEVTISGAKNAAVAIIPAVILCDEPCQIENI
                     PNISDVSLISRILQQMGANVRRINRSSLYIDPTKIRTCNAVTDLVRGMRASYYLLGAL
                     LGKYGKAKVALPGGCNFGVRPIDQHLKGFEALGATTQLVDGAIIEAECDSLVGTHVYL
                     DVVSVGATMNIMLAASKAKGQTIIENAAREPHIVDLANFLNSMGADVRGAGTDVIKIR
                     GVEYLHGITYSIIPDQIEAGTYMCAAAATRGCITVKNITPKHLESISAKLREMGVKIT
                     EYDEALKVDASSAPLKRCNIKTMPHPGFPTDCQPQFAALLMSVPGTSIINENVWDNRY
                     QYVSELIRMGAQISVEGRLAIIEGGVPLTAAPVKATDLRAGAAMIIAALEINGTTEIS
                     NIQFIERGYEDVVEKFRGLGADIKKVYFTGDNEQELGA"
BASE COUNT         2819 a         2292 c         2078 g         3132 t
ORIGIN      
        1 gtcggctctt ctgttgccgg ctcggtaaca ggggcggttg tcggagtaac ttccggctca
       61 tagtaagtgc cgacatttcc gtgaccgtca agctttgcga catacatctg aatgtatgtt
      121 gcgtctttaa cgcttacgat atcattctcg ccgtcagcgt cacatcttgc ggctgcgata
      181 agagccttat ctgagagtgt tataagcttc acaatgtact tctgaaccgc tgttgcgtca
      241 agtacattaa tcgcaccgtt gagattaaca tcaccgataa ggattttttc cgtcggctgt
      301 gtaacggggg ctgtggtagg agttaccgtt gaaggagcag ttgaaggacg ctccgatgat
      361 gtgtaaagat aaacaacatt gataataccc gatgaaaggt tgcctgacgc atttacggga
      421 gcaataacat ctgttctgcc cgaaagcgga gaagttttgt atgtatcgtc ggaagaaacc
      481 tttgactgaa ttaaaatttc atcatcggca atcttttcac ctgttgcggc gtcaatgtgg
      541 cttgtgataa ccttactgct aaagctgagt ttcttattct gaacccatgc ctcgcccgaa
      601 acaaggtagc ctgtctcgtt tgcacccggc tcctgctgtg agtttgtgtg gaacattaca
      661 tatgagcttg gtgcaggaat tgtgcatttg agccatccgt tgccctcact tgtcataaca
      721 gttgcgttgt tccacttgcc gttaggctct tcgccgccgt ctgtgtaagc atagcaacga
      781 acatttgacc agttgtttga gttatagtag tgaacaaccg aacttgtgct gtcaagtgcc
      841 ttataaacaa agttaatggt tgtgttcgta ccgttaaatg tacctgtcgt gttggcagga
      901 tacttgtctg tgtcaagctg atagccctga attgatgtga acggaacgct gtagctgtcg
      961 ccttcacgat actttgttga aacggtatcc ttaattgact caccgttttc gtcaacataa
     1021 tttaccgtaa catagcctga acgaataccg ctggagctgt aaacataagt tacattgtaa
     1081 ttctttccgc ctgtaacagt acccgaagtt acaccctgag tatccttgag agtatagtca
     1141 taaaggatgg ttgtgtcggg gtaagtacgg tatgttgtac cgtctgcata ttttgctgtt
     1201 gatgttttga gaacatttcc gctgtcatca atatgtttga ttgttaccgt accgaatgtt
     1261 ttttcactaa ccttaaggtt gttgaatgtt gaactctgaa caagcacaca ggcgctgttt
     1321 gcgggaactg tatatgtgtt gcccgaaact gtgccaagac ttttaagacc tgccgtcgta
     1381 ccgtctgcaa ctactgtcca gccgctgccg tcaagtgtaa tcggccaatc atttgttgaa
     1441 cttgcgttta caagaacaca aaccttgccc cactcatttg acttgttgtt tgaatatgtg
     1501 taagcaacaa catcaccgtg tgttgactgg aatgaaggag tgttgaatgt tgaactcttc
     1561 atcggagaat agttttctct gatttcaaga agtcccttgt aataagcggc aacatccgag
     1621 taggtcttta ctcttgacca atcaatctca tttattgcgt cgcttgattt gtaactattt
     1681 gtatcaccct gttttgtacg gcaaaattcc gagcctgccg tcataaacgg gataccctgt
     1741 gaagtaagga ggagtcccat tactttctta acctgtcccc tgagtgatga agatgttgaa
     1801 ttccagcttg atgagccgtt actctttaca attttatccc agaggataag gttatcgtga
     1861 gcgtcggcat aagcaattgt ctgtgaagga gcctgagccg aaaagccctt gccggttaca
     1921 ccgtttgcaa ccgttccggc cttatcggtg ttgccctgaa caaatccctt atcggaaccg
     1981 tctgtgcttc ccttaattgc atcacggtag ctgtcgcaga acatacctac tcttgaattg
     2041 agtgaacctg cctttgactg cgagcaaccg tcggaaattg caactgaacc gcctgtccac
     2101 ggttcaccgt acatgagaat ctttttaccc gaaccgtctg aataaagtcc gtcaagagca
     2161 gaacggatat cgttcattgt tgtgatatcg tgaataccca taaggtcaaa acggaaaccg
     2221 tcaatgtgat attcctccgc ccaatacttt acggattcaa tcatatactt tctgtacatc
     2281 agcttatctg acgcagtttc gttaccgcag cctgaaccgt ttgaatatgc tgatgatgaa
     2341 tgcattctgt agtagtaacc cggaacagtt ctgttaaagc aggaatcggt tgagaatgtg
     2401 tggttgtaaa caacatccat tacaactgaa attcctctgt catgaagagc ctgaatcatc
     2461 tgcttgaact cagtgattct tgtgttgccg tcatatgggt ttgttgaata tgaaccctcg
     2521 ggagcgttat agttcaccgg atcataaccc cagttacggt ttgaagaaga acttgcaaca
     2581 tcttccttta ccgaaccgta atcataaaca ggcataagct gaacgcagtt aatgccctgc
     2641 tcaacaaggt agtcaatgcc tgttgaaacg gctgatgaac ttgtgtctga attaagagtt
     2701 gtgccgccct cggcaaatgc aaggtactta cccttgttat cctcactcac acccgagttt
     2761 ttggaaactg aaaaatctcg cacatgaact tcccatacag ccgcctcgga tgcgctgtta
     2821 aagagaacat gcttatcgtc cgaccaaccg ctcggatcgg ttgagtcaag gtcaacaacc
     2881 attgtacggt tgccgtttac accgacagcc tgtgagtaaa catcctgtgt ttccttggtt
     2941 gtgccgttta ctgttacaag atatgtgtaa tatttgtttt tgtaatcacc gctcagatta
     3001 agtgaccaaa cacctgttga gctgtctttt ttcatatcat aggtgccgat tgccgaagcg
     3061 cccgcctcgt tgtcagaacc tgttgtgtag agcttgagct ttacggatga cgcatcaggt
     3121 gaccaggttt tccatgttgt ggaagttttg ctgtacactg aaccgagtcc cgattcgtta
     3181 tacgctttgc ttgcatagct ttcaagataa tttgcattgt aataattgcc tgctgaaacc
     3241 gattcatcgc ttgttactgc cgcatttgag gcaaaggccg acatacctgc aatcatggac
     3301 agagaaagaa gaatcgataa tggtttgcgt aatctcatat tttcactcct aaatattatt
     3361 tcgcacaaag taccgtacga taactatatt ttaacattta taaatgccgt atttttccta
     3421 ttgagaacta aaaaagcatc ctaaagccaa actttaagaa cgctttttat acattatgca
     3481 caaataaaga ctgatgcaaa gcaatgatta actattgtta ttgcaatatt tcagcagtta
     3541 actgtcgccg atatattatt tgccgtttgc cgaaaaatct gactgcactg aaattatata
     3601 ccatatgtaa tactttgtta catattatta agattttatc gaactaaagt ttttattgtt
     3661 atataattct ttaactgtgg tttacattca tttgtcatta tgtacaattt attactgata
     3721 catcaccatt tgtttacaac ttaacaatat ttttgtaata atattgacaa tatcgcaaca
     3781 taatagtatt attatcttgt gaccggttgt catccggagg gaaaaaatga taacctacaa
     3841 attgaatcat acacccgaaa acgggtttaa cacctttggg ataagcgcat tttgcggcaa
     3901 aaagcttata aaatccgtgt cgggaatcag cgaaagcttt cgtgaaatga cggagcttgt
     3961 cgaaatgtgc aacgaacttg agcttgaact ctgccagctt gacgatgtca tcgaagatta
     4021 tcttactgat ttcacacttt aatttgcaaa gactttaact tttgtcataa tcgtggtaca
     4081 atagtgttta atgttctatc agacattata tatcagacaa taaggtgata tcatgatttg
     4141 caatttatgt ccgagaaaat gcggtgcggt tcgtactgcc cacagcggaa acggattttg
     4201 taaaatggga gaactgcccg ttgttgcaag agttgctccg cactatggcg aagaaccgtg
     4261 cataagcggc acaaagggca gcggaactgt tttcttttcg ggctgtacaa tgagatgcgt
     4321 gtactgccag aactacgaaa tttcaaacga ggacggcgga agaacaatca ctcccgaaag
     4381 acttgccgaa tgttacaaag aacttgagga caagggagtg cataacatca accttgtaac
     4441 ggctgaccat tttgcacacg ctgttgttaa aagtcttgaa atttacaaac cgagaattcc
     4501 gattgtatat aactgcagcg gatacacaag tccgaaaact ctcagtatgc ttgacggact
     4561 tgttgacatt tatcttcccg actttaaata tgcagatgat tttcttgcgg taaaatactc
     4621 ttccgcaccg aattatgtca acacggctgt tgccgcaatt cacgaaatga cttttcaggt
     4681 tggtctgcct cagtttgacg atgacggcat tatgaaaaag ggcgttatcg taaggcatct
     4741 gattttgcct gcccacacaa agaacagtct tgaagtgctt gatattgtaa aacgcagatt
     4801 cggcgatcag gttttgttaa gtcttatgtg tcagtacatt cctatgggca aggtcagcga
     4861 cagggagttt tcacgaatta accgtaaaat cacacgcaga gaatatgaca aggtaaagct
     4921 tgaaatgatg cgactgggaa ttgacggctt tacacaggag ctttcttccg cagatgaaag
     4981 ctatgtcccc gaatggaatt ttgaaggata attttgtcag ttcggtcatc tgaattcctc
     5041 ttcatttttc aattggtatt gtttacttgg tattattctc ttagtctttt tctatgggta
     5101 ttattaggct tcaaattctg aagtagtccc cttcaaaatc tgaaggggac gggttcaaat
     5161 tctgaagggg gcaggtgcaa attttgaagg gtatgagttc aaattctgca cccctaaaat
     5221 acgaattttt acattcgatt ttaccgcccg ttaaaaacaa ttaaattaac ataaaaacgg
     5281 agagcgtctt gccctccgtt taatttttat taaaatatct tactgctttt caaccgtttg
     5341 tgacggtttt acatacaggg ttttgttgtt gacataaatc accatacccg gttttgagcc
     5401 gtttggcttt gacacatagc gaacaagtgt gcagtcaacg ggaacctgac tgctgtttct
     5461 cgccttactg tgatatgccg caagccgtgc cgcctcatag attgccgtgt cggaaatctc
     5521 tttgccgtcc gaaacaacaa ttacatgaga accgtgaatg tcctttgtgt gcagccacat
     5581 atcgttcttt gaagctgttt tcaaagtcag cttatcattc tggcggttgt ttcttcctac
     5641 aaggatttta aaaccgtcgc ttgattcaaa ttcgagcggc ggcaaagttg tctgacgggg
     5701 ctttttgccc ttttgaacct tgagatagcc ctgctcggta agttcctcac gaatttgtgc
     5761 aaggtctttt tcattctccg cacggtcaac cgtatcgaga accgagttaa tataatcaag
     5821 ctcttccctg cccttttcaa gctgttcttt gagcataatt tcggcatttt tagccttgcg
     5881 gtaatccttg taatacttct gagcatttgc cgaaggtgag attgccggat taagctttat
     5941 gcgtaaaatt gcgttgttct catcgtaaaa gttttcaaca tcaacatact ccgcaccacg
     6001 ctcaatgcga tagatatttg cctgtaaaag gtcaccgcat atgcgaagat gctcacggtc
     6061 tgcacagcgt tcaagctcac tcttctgctt tgcaattttt cgtgcaagcc tttcaagtgt
     6121 gttgttcaaa agctttgtaa ggctttgtga tttcaccctc attctgtcac gactgtcacg
     6181 agcctcatag aatccgtcaa gcaaatcgga gaagctttcc ttactctcaa cctttgcaaa
     6241 atcgccgtac tgctcaacat tcataaagca gaagtccata ggcttgccgt cctcacgata
     6301 aaccacaaca ggcttgcccg aacaatcgct gcaaacgctt ttcagctttt caatttcaac
     6361 tgcgagtcgg tcaaaaagca caccctcaat tctgtttgtt gcgccctcca taaccttgta
     6421 ttcaatctca cggcatacaa tcggagaaac gccctcaatt gtccttaaca gcgccttgtt
     6481 aagcggcatt tccgtgtcaa gattttttac ggctgtacac acatcaagca ccgaacattt
     6541 aagcagattc agcttgttct gcgattcggg aagttcgtat tttatattcg gcagtacaag
     6601 tcttttactg ctcattgtaa gatctacacg cttgagtgaa tcgatgatga cgccgtttga
     6661 atcgacaata attatgttgc tgtacatacc cataatttcg catacaacag ttatcatcac
     6721 agtatcgccg agttcgttca cgcactcgaa atcgagcata agaacacggt cgcaaccgtt
     6781 ctggcgaagt cccacaagct ttccgccgcc gattcttttt cgcaaaagca tacagaacat
     6841 cggaggttgt gcaggatttt cgggtgttga cgaacagaag ttcactctcg gtgaatttgc
     6901 tcgtgcagaa agcaacagct ttttgttgcc ggaaaatgtt ctcagcccga acacaagctc
     6961 ctctctgttc ggctgataaa tctgcgacac ccttgcaccg agagcctctt tttctatttc
     7021 acttttaata tgtcttaaaa aaattccgtc aagcgccata gtaactccgt aaattatttg
     7081 tgatatttac cgtttgatga aatctcaaac gcacggtaaa tctgttcgct taaaatcatt
     7141 cttgccattt ggtgaggaaa ggtcattctg ctcattgaaa gacggacatc cgcccgtttt
     7201 ttaaccgatt cgtcaagtcc gtagctgccg ccgataataa aatcaacggt gctttttccg
     7261 cttacggacg cattgtcaat cagctttgaa agctcctcac tcgacataga ctttccctca
     7321 atacacattg caacaacata atccgaaggc gaaattttct gcatgattcg ttttccctca
     7381 gagaaaataa cctcatcaat ctgcgaccta ttcggtatgt tgctcttaat gcgttcttcc
     7441 gaaagctcaa taatattgaa tttacaaaat gcggtcaagc gttttgcata ctctgcaatt
     7501 gcgtcggtaa aatatttttc cttgattttg ccgatacaaa ttatgttaat cttaatcata
     7561 ttcctcttgt ttgtttaagg ttaaaagatg atggcttttg ttgtgcttac actcggagca
     7621 acatcaagcg aataatcgct tttgtacttc attccgtttg atgtgagcag gtctacggat
     7681 gttgacattg caagcggaac ggtattattt tcttcgctga gatggccgag cataagtctt
     7741 aaagttccct tttcaacaag gttaacaagt tcttttgcac acgcctcatt tgaaagatgt
     7801 cctctgtttg aaaggattct ctgctttagc ggataaggat aaaatccgtt tttgagcata
     7861 ttgatatcgt gatttgactc aagcacaaca aagtcgctct gctctgcggc agttctgaca
     7921 tcgggcaaca taacgcccat atcggttgct ataagtgcgg attttccgtc gggagcagta
     7981 acacggtaac aacagctttc cgccgcatcg tggggggtgt caattcgctg aacacgcata
     8041 ttgccgatta caacctcgtc agttataaca tctgcatccg tgtttggatc aagcttatta
     8101 cctgccctca aagcattgag cgtaccaaga cttgaataca ccttaaagcc gtaacgcttt
     8161 gcaagcacac gcaacgcact gcaatggtca gtgtgttcgt gagttataaa aaccgccccg
     8221 atactgccca tatcaagtcc gtttgcaagc atggcatttt caatctgctt gcatgaacga
     8281 cctgcgtcaa tcagtacgcc ttcgcttgcc gaacctatgt agtagctgtt acccttactt
     8341 ccgctgaaca gcggtactgc tcttgccaaa acaatcaatc cttatcttta gtataacgaa
     8401 tatgataaca caaattttaa aatttgtaaa ttatctcaca aaaaagccgt tctcacaaag
     8461 aaaacggctt tgatttctaa ttaaatttta gtcaggcacc aagctcctgc tcgttgtctc
     8521 ctgtaaaata cacctttttg atatctgcac caagtcctct gaacttttca acaacatctt
     8581 catatccacg ctcaatgaac tgaatattag aaatctctgt tgtaccgtta atttcaagag
     8641 cggcaataat catagccgca cctgcacgaa ggtcggttgc cttaacggga gccgctgtga
     8701 gcggaacacc gccctcaatg attgcaagtc tgccctcaac agaaatctgc gcacccattc
     8761 tgataagctc gctgacatac tgatagcgat tatcccaaac attctcgttg atgatgcttg
     8821 ttccgggaac gctcataagc aatgcggcaa actgcggctg acagtctgtc gggaagccgg
     8881 gatgcggcat tgtcttgata ttacagcgct ttagcggagc agatgacgca tcaaccttga
     8941 gagcctcatc atattccgta atcttaacac ccatctcacg cagctttgcg ctgattgatt
     9001 caagatgctt cggtgtaata ttctttaccg taatacaacc tcttgttgcg gcggcggcac
     9061 acatatatgt tcctgcctca atctggtcgg gaataatcga ataagtaatg ccgtgaagat
     9121 actcaacgcc acggattttg ataacatccg tacctgcacc cctgacatcc gcacccattg
     9181 aattaaggaa gttcgcaagg tcaacaatat gcggttctct tgcggcattt tcaataatgg
     9241 tctgtccctt tgcctttgat gcggcaagca taatgttcat tgttgcacca acggacacaa
     9301 catcaaggta cacatgtgta ccgacaaggc tgtcgcattc tgcctctata atcgcaccgt
     9361 caacaagctg agttgtcgca ccgagagcct caaagccttt aagatgctgg tcaatcggac
     9421 gcacgccaaa gttacagccg cccggaagag ccaccttagc cttgccgtac ttgcccaaaa
     9481 gtgcgccgag aagataatat gacgctctca tacctcttac aaggtcggtc acagcattgc
     9541 atgtgcggat ttttgtagga tcaatatata aggacgaacg gttgattcgt cttacattag
     9601 cacccatctg ctgaagaatt ctgctgataa gtgatacatc gctgatgtta ggaatatttt
     9661 caatctggca cggttcatca caaagaataa ctgccggaat aattgcaacg gctgcatttt
     9721 tcgcaccgct gattgtcact tcacccgata atggctttcc gccggtaact acgaacttct
     9781 ccaattgtta caccccaatt aatgtgtaga agatactttt aactaaataa gctaaatatc
     9841 tgaaaatgta tatctatata gtgtataagt aaaaatcaaa aattatacga atctaaaatt
     9901 cataatgaga actaatttgt tacattttat taaaaacgaa cccactcggt tacgcagatg
     9961 taatttaaca attctcttac agaaaatgat actacaaaat tgtagtaaag tcaacggaat
    10021 aataaaactg taatctattt ttgggcaagt tgtactcaaa ttattaattt gttgcttttt
    10081 ttgtttttaa tataaccaac catgcttttt aaaaccgttt tttcctaaaa agaagtaatt
    10141 aataaaagca attttttgta aaattattac aaattgaaac aagtgttatt ttcgtgttat
    10201 catcaggcat ctgagcattt tacaattcaa attgaacaca gaaaaatatc gttccgttat
    10261 tcctatgata attttacacc ttcaatcttc ttttttcaaa tttaaaatta actttctata
    10321 c
//
LOCUS       DAOMVE010000060        10047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1658_length_10047_cov_2.760208, whole genome shotgun sequence.
ACCESSION   DAOMVE010000060 DAOMVE010000000
VERSION     DAOMVE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_10047_cov_2.760208"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..434
                     /locus_tag="PLS64_04785"
     CDS             252..434
                     /locus_tag="PLS64_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43178.1"
                     /translation="MFWLVFWFLVCVAILAYGIRRMANHDYRFGSGLFKSSDSNLDEK
                     DEFVDKDYYDKKNLFK"
     gene            complement(520..2664)
                     /locus_tag="PLS64_04790"
     CDS             complement(520..2664)
                     /locus_tag="PLS64_04790"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HRL43179.1"
                     /translation="MKNKTSFTIDILLCITLISSLFYCLVTSFDIKTEWYIIVVPTAI
                     FCTIYGLLASLVKKSGVLFISVAITMIVFVFTLLFSLESILEQLSYVITQALKLLSQY
                     LPVQNKIILGDTLGTSATGLFVFISAILSAVFTISLIRYKRILIPSLLSMVLLVPCFI
                     LVNTLPSLVCLFIAIASLIALYISKNTRKFRPSQGGVIAAISFGTMLVVMAILLAFNP
                     IEKYERFDWQENLLQSIENGFNIDDGRKTSTEKNLNEIKGSLEEKENLNDISPLTQTH
                     TPVMTVTSAVGGNIYLKGTAYADYRDNIWSIISDNDAARYPQDFVPFTATIGNNALTD
                     TRVHMLNESKIAYVPYFLESLPDNLNSVADVCVKNDTDKNEFNYSHYPFYAENTYRYN
                     NSAEAENYKAFVYDTYLEIPRKTQIELRRIADQEGFLDSELTVSEKIEMVKNYISNSK
                     TYSLKTPKMPSGKDFATWFLNESDTGYCVHFATAGTLMLRALGIPARYVTGYYATAYA
                     NQTVTVTTDNAHAWVEYYSDTAGWIPLECTPSSFQPARYVGSQRANGGSTDTTQPTTV
                     EPTTAPTAPTNGTVSHSTKPKNNSKSSFEFNGFTIAMMSLGIVALALIAIFSRRIIII
                     KKRKQRFETGRNNKRAICIYKELKNLTKISHLLITDEVKSVYEKARFSNHTISDDELK
                     IIENAYYNSIEEIYNNLPRIKKPYYKFIKAIK"
     gene            complement(2661..3623)
                     /locus_tag="PLS64_04795"
     CDS             complement(2661..3623)
                     /locus_tag="PLS64_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRL43180.1"
                     /translation="MIRNIIIYLCLLTGTFLFSIFYYAWFSWFLFLTVVSIPIISLLL
                     SLPFMIRSVRSDIEIFAKENVFIKDEFYIAISGKKKKPLFCPMLTLNIKSTNLTANLC
                     ENIKIRFSGTFTKPLIRQCNSLTQHCGQLDIVCKNGKVYDMLGIFFLPIRIKKRSFVR
                     VLPKQQKPKIIPDSSNFTVTGYKPKPVGNSEIYELREYRHGDSLRNVHWKLSSKSDGL
                     IVKEPNIPIIKNCLIMPFFGDNADENDVVFAKLMYVCRYMIKSIGICFACDRNGNPFE
                     IRCESDILNYFSALYLNTESSFVADTNDFQHYSIFADREEVDLL"
     gene            complement(3626..4552)
                     /locus_tag="PLS64_04800"
     CDS             complement(3626..4552)
                     /locus_tag="PLS64_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HRL43181.1"
                     /translation="MNKFQLITNEVKKVINGKDRAVVTTLLALLTGGNILIEDIPGVG
                     KTTMAVAFSKALGLEYGRVQFTPDTLPSDITGFAVYNKDTGKFTFNKGAVFCNLFLAD
                     ELNRTSSRTQAALLEAMEERQVTVEGNTFKLEKPFSVIATQNPTGASGTQLLPDSQID
                     RFTVRLSMGYPDSDAECKMLLNRSGKNPLNSVNCIVSKNEFLEMQSEVQNVFVSDDMA
                     RYIVSLISATRKHPLLSRGASPRATLSLTDMAKAVAFAEGRDYIVPRDVQNIFICTVN
                     HRIILNAKASASKKNSEEILHEILRKTPKPRT"
     assembly_gap    5034..5043
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5150..5998)
                     /locus_tag="PLS64_04805"
     CDS             complement(5150..5998)
                     /locus_tag="PLS64_04805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL43182.1"
                     /translation="MNRMPVAAIMYDFDRTLCTKDMQDYSFIPRLNMTEDEFWKFSNE
                     LGQSEHMDSILAYMYAMVKMSRERNMPLLRNDLVEMGKNVEFFDGVKDWFKRISDFGE
                     KLGMQVEHYVISSGMKEIIEGTEISKNFKSIFACEFLYDENGNAVWPKTDVNYTNKTQ
                     FVYRINKGVLDVANDVDLNRSMPEDSKRVPFCNMIYIGDGLSDVPCMKMMKAYGGYSI
                     AVYRKKDSKVEDLLMKDRVDFIYPADYSENTGLDLTVKNIIRKMAVCGLLYDENHEQK
                     KEILGR"
     gene            complement(6008..7021)
                     /locus_tag="PLS64_04810"
     CDS             complement(6008..7021)
                     /locus_tag="PLS64_04810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRL43183.1"
                     /translation="MNTYNLKKMCAVSVASIMCVSALAFSGCGDKKAEVSQTPDETTP
                     ATTAVSTADEPTAQNYLESIGIDTATLGINPNIIHDSNPVGFQLDMPKNGDTIAVVHT
                     SYGDITMRLFPEQAPKTVTNFVNLAKAGKYNNSLFSKVTKDFMINGGYCGTSSYGEAF
                     EDEFCDRLFNIRGAVAMSNTGADTNESAFFINQKTAETYKNEGGWEHLASQWDSIKTQ
                     LANYKDSNLLTAFIEKNGTNCYDTDSVPDSVKKLYEENGGNAYLDGAYNAVDRGYTVF
                     AQVIDGMDVVDKIASAKVDKDDIPVKNIVIKSIKITQYKEESTTQADSTSAKSVTQPT
                     TVG"
     gene            7427..8353
                     /locus_tag="PLS64_04815"
     CDS             7427..8353
                     /locus_tag="PLS64_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRL43184.1"
                     /translation="MSEYLPEGKLISEQENINILHSLKMLEDAKNSEKILEAKACVCD
                     SSHNLIVDLAGIKGIIPREEGAIGIKDGSVRDIAVISRVNRPVCFVVTDITEDENGKP
                     IVMLSREKAQRKCLDAYISRLRCGDIVPARITHLENFGAFADIGCGIVALMPIDTISV
                     SRIEHPAERFTVGMDIKAVIKSVENGRISLSHKELLGTWEENAERFCAGETVSGIIRS
                     VENYGAFVELTPNLAGLAETREGVRAGQQASVYIKSIIPEKMKIKLIIIDTFDYKYNP
                     KCPEYYCDSDRIDRFVYSPENCRKRVETIFDT"
     gene            8422..9591
                     /gene="rmuC"
                     /locus_tag="PLS64_04820"
     CDS             8422..9591
                     /gene="rmuC"
                     /locus_tag="PLS64_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRL43185.1"
                     /translation="MQFTTSEIIVLLFCAITLIVSVVTLITVLKKNNNNRTENELQRI
                     LLNQQSFAQSQNDRAIQLEQRLHSFSADNVQSLENIRRSVDEKLESIRRENLRQLDEM
                     RQTVDEKLQKTLEEKMNKSFSLVNERLEQVYKGLGEMQTLAVGVGDLKKVLSNVKTRG
                     ILGEIQLGAILSEILTKEQYEENIATKKGSKNVVEFAIKLPADGAGTVYLPVDSKFPG
                     DTYSALRDAVESCDKQRIESAQKALVQRIKGEAKDIHDKYIDPPNTTEFAIMFLPFEG
                     LYSEVVNMGLVEVLQREYKVNIAGPSTMAALLNSLQMGFKTLAVQKRSAEVWKILGGV
                     KTEFDKFNDVLVMTQQRLDQANKELDKLVGVRTRQIQRQLKDVESVTLSEQNLFE"
     gene            complement(9649..9954)
                     /locus_tag="PLS64_04825"
     CDS             complement(9649..9954)
                     /locus_tag="PLS64_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43186.1"
                     /translation="MKKKAARIFTAFIMTAIIAQGTVMVANAAETDSTPDSIAVVEVA
                     TPDSSALESGADSTSVQPIADVIYKKWRRGSHGQLQYRRWNDTKQEWVDDEWIDYKG"
BASE COUNT         2914 a         1988 c         2039 g         3096 t
ORIGIN      
        1 aatctgtgaa gttaaacagc aaaagtgtat tacaaactac cgcatttata aggaattgaa
       61 ccttaatgca ggtaatgtca attcttttct caaatacggc aacactgata aattgagtgt
      121 tgaaacagta agaaaagttc ttacatttat taatgaatat taataaaaat atattgacaa
      181 tataatattg caatgatata atcagctaaa caaaaatatt aacattgtat tgcttttgag
      241 aggtgttgtt tatgttttgg ttagttttct ggtttttggt gtgtgttgca attcttgctt
      301 acggaattcg cagaatggca aatcacgatt atcgttttgg ctcgggactt ttcaagagca
      361 gtgacagcaa tcttgacgaa aaggacgagt ttgtcgataa ggattattat gacaaaaaga
      421 atctttttaa ataatataaa gcaaaatcta aggctcttga gttgttttca agagcctttt
      481 gttgtgtaaa aacgctcccg tattgtacgg gagcatttct tattttattg cctttataaa
      541 cttgtaatac ggctttttga tgcgagggag attattgtaa atttcttcaa tggaattata
      601 gtaagcgttt tcaataattt tcagttcatc atcgcttatc gtgtgattgc tgaatctcgc
      661 cttttcgtac acggatttca cttcatctgt aatcagaaga tgcgaaattt tcgttagatt
      721 tttaagttct ttataaatgc agattgccct tttattgttt cttcctgttt caaatctttg
      781 ttttctcttt ttgataatga taattcttct tgagaaaatt gcgattaaag caagggcaac
      841 aattcccaat gacatcatag cgattgtaaa tccgttaaat tcaaatgaag atttactgtt
      901 atttttcggt tttgtgctgt gagaaaccgt gccgtttgtc ggagcagtcg gtgctgttgt
      961 cggttcaacg gttgttggct gagttgtgtc cgtactgccg ccgtttgctc tttgagagcc
     1021 gacatacctt gccggctgaa acgatgacgg tgtacattca agcggaatcc aacccgccgt
     1081 atcgctgtaa tactcaaccc atgcgtgagc gttgtcggtt gttacggtaa cggtttgatt
     1141 tgcgtatgcg gttgcatagt agcctgtaac atatcgtgcc ggaatcccga gtgccctcag
     1201 cataagcgtg ccggctgtgg caaaatgaac acaatatccc gtgtcgctct cgttgagaaa
     1261 ccaagttgca aaatctttgc ccgacggcat tttcggagtt ttcaacgaat aggttttact
     1321 gtttgaaata tagttcttta ccatttcaat cttttcggaa acggtaagct ccgagtcgag
     1381 aaagccctct tggtctgcaa ttctacgcaa ttcaatttgt gtttttctcg gaatttcaag
     1441 ataggtgtca tacacaaatg ctttgtaatt ttccgcttcg gctgaattat tgtatctgta
     1501 tgtgttttcg gcataaaacg gatagtgcga ataattaaat tcgtttttat ccgtatcgtt
     1561 ttttacgcac acatcggcaa ccgaattgag attgtcgggt agactttcca agaagtaggg
     1621 aacataggca atcttacttt cattcagcat atgaaccctt gtatcggtta aagcattgtt
     1681 tccgattgtt gccgtaaacg gtacgaaatc ctgcggatat cttgcggcat cattgtcgga
     1741 aatgatactc catatattgt ccctgtagtc ggcatatgct gtgcctttga gataaatatt
     1801 accgccaact gccgaagtga ccgtcataac aggtgtatgc gtctgcgtca gcggagaaat
     1861 atcgttgaga ttttcttttt cttcaagtga acctttgatt tcattaaggt ttttttctgt
     1921 ggaagttttt cgtccgtcat caatattaaa gccgttttca atgctctgca aaaggttttc
     1981 ctgccagtca aatcgctcat atttttcaat cggattgaac gcaagcaaaa ttgccatcac
     2041 aacaagcatt gttccgaatg aaattgccgc aatcacaccg ccctgtgacg gtctgaattt
     2101 gcgtgtgttc tttgaaatgt acaaggcaat aagacttgca attgcaataa aaagacaaac
     2161 gagcgacggc agagtgttta caagaatgaa gcaggggaca agcagtacca ttgacagcaa
     2221 tgacggaatc aaaatacgct tgtatcgtat caggctgatt gtaaaaactg cactcagtat
     2281 tgccgaaata aacacaaaaa gtcctgttgc gcttgtgccg agggtatctc ccaaaataat
     2341 tttgttctgt acgggaagat attgcgaaag cagttttaaa gcctgagtaa taacataact
     2401 caattgttca agtatgcttt caagtgaaaa cagcagagta aatacaaaaa caatcatagt
     2461 gattgctacg ctgataaaca gtacacccga ctttttgaca agggaggcaa gcaatccgta
     2521 aattgtgcag aaaattgccg taggaactac tatgatgtac cattctgttt ttatatcaaa
     2581 agaggtgaca agacagtaaa acaggcttga aatcaatgtt atgcagagaa gaatgtcaat
     2641 cgtaaagctt gttttgtttt tcacaacaag tcaacctcct cacgatcggc aaatatacta
     2701 taatgctgaa aatcatttgt gtcggcaaca aagctgcttt ccgtgtttag atagagtgcg
     2761 gaaaaatagt ttaatatatc gctttcgcat cttatctcaa aaggattgcc gttcctgtca
     2821 caggcaaagc aaatgcctat gctcttaatc atatatcggc agacatacat cagctttgca
     2881 aaaacaacat cattttcgtc tgcattatct ccgaaaaaag gcattataag acagttcttg
     2941 attatcggaa tattcggttc ttttacaatc agcccatcgc tttttgacga aagcttccaa
     3001 tgaacattgc gcagactgtc accgtgacgg tattcacgaa gctcataaat ttcgctgttg
     3061 cctacaggct taggcttgta ccccgtaacc gtgaaattac tgctgtcggg gataattttc
     3121 ggtttttgtt gttttggaag aacacgaaca aagctacgct ttttaatcct gatcggaaga
     3181 aaaaatatgc cgagcatatc gtaaacttta ccgtttttgc atacaatgtc aagctgtccg
     3241 caatgctgtg taagactgtt gcattgtctg attaacggct ttgtaaatgt acccgaaaat
     3301 ctgattttta tgttttcgca aagatttgct gtaaggttgg ttgattttat attcagcgtg
     3361 agcatagggc agaagagcgg tttcttcttc ttgcctgata ttgcaatgta aaactcatcc
     3421 ttgataaaaa cattttcttt tgcaaaaatt tcaatatcgg agcgtacact ccgtatcata
     3481 aaaggcagac tcagcaaaag cgagattatc ggaattgaaa caaccgtcag gaacaaaaac
     3541 catgagaacc aggcgtaata aaaaatgctg aacaaaaatg ttcccgtcag cagacaaaga
     3601 taaattatta tgttccttat cattttcaag tcctcggctt tggagttttt ctgagaattt
     3661 cgtgcagaat ttcttcgctg ttcttttttg atgcagaggc ttttgcgttg agaattatcc
     3721 tgtggtttac ggtgcagata aaaatattct gcacatctct cggcacaatg taatctctgc
     3781 cctcggcaaa tgcgacagcc tttgccatat cggtaagtga aagcgttgct ctcgggcttg
     3841 cacctcttga aagcaacgga tgctttcttg ttgccgaaat gagtgacaca atatatcttg
     3901 ccatatcgtc gctgacaaaa acattctgaa cctccgactg catttcaaga aattcatttt
     3961 tggatacaat gcagtttact gagttaaggg gatttttacc gcttctgtta agcagcattt
     4021 tacattccgc atcgctgtcg ggatatccca ttgaaagcct taccgtgaat cggtcaatct
     4081 gtgaatcagg caaaagctgt gttcccgacg ctcctgtcgg gttttgcgtt gcaataacag
     4141 aaaaaggctt ttcaagcttg aaagtgttgc cttcaacagt aacctgtcgt tcttccattg
     4201 cttcaagcaa agccgcctga gttctgcttg aagttctgtt taactcgtct gcaagaaaaa
     4261 gattacagaa tacagcgccc ttgttgaaag tgaattttcc tgtatccttg ttatagaccg
     4321 caaagcccgt gatatctgac ggcaaagtgt cgggagtgaa ctgtactctg ccgtattcaa
     4381 gaccgagcgc ctttgaaaac gcaaccgcca ttgttgtttt tcccacaccc ggaatatcct
     4441 caataagaat gtttcctcct gtgagcaacg caagcagagt ggtgacaacc gccctgtctt
     4501 taccgtttat tacctttttt acctcattgg ttataagttg aaatttattc attatttgtc
     4561 cctttgtgtt aaatttgttg ttatattttt tatacaataa ttgaatctga gcaacgctct
     4621 ctttaaatta tatttgattt gcttttttat tgcaagtctt tttcgtaaaa tacagaccaa
     4681 ctacagatgt gaagatatgt ttttaggtta tgcagaatat atgcaaaaaa acggcagtcc
     4741 gtaaagaact gccgtctgaa aattaaattg acaaataaga attaaactgt tagaataaaa
     4801 ccgtgatacc tgcattaaac ggtaggcggt ttctcctcac aagaggaggt gataaccatg
     4861 acaatttccg atgtatgttt actcggcact ttaatcgtca gcgttatcgc tctttgttac
     4921 caagtctttg gcaataaaaa gtaattttgt ttcttcgtac atatatgtac atttaaataa
     4981 aaaaagaaac taaccgccca aaattctccc aacggcggtt agcttctaac caannnnnnn
     5041 nnnagaatcc gcttatcgca ggtatctcct tttctataat tatattatac caaataaata
     5101 aaaatgtcaa gcatcgtttt gatacttgac attttttatt caaccgaaat tatcttccga
     5161 gaatttcttt tttctgctca tggttttcat cgtacaaaag accgcagaca gccatttttc
     5221 tgatgatatt tttaacggtg aggtcaagac ctgtgttttc cgaatagtcg gcaggataga
     5281 taaaatcaac tctgtctttc atcagcaagt cctcaacctt gctgtccttt tttcggtaaa
     5341 ccgcaattga atacccgccg taggctttca tcattttcat acacggaaca tctgaaagac
     5401 cgtcgcctat gtaaatcata ttgcagaacg gaacccgttt gctgtcctcc ggcattgaac
     5461 ggttgaggtc aacatcattt gcaacatcaa gaacaccttt gttgattctg taaacaaact
     5521 gcgttttatt ggtgtaattt acatcggttt taggccatac ggcattgccg ttttcatcgt
     5581 aaagaaattc acatgcaaaa atgcttttaa agtttttgct gatttccgta ccttcaataa
     5641 tttctttcat acccgaggaa attacataat gttcaacctg cattccaagc ttttctccaa
     5701 agtcggaaat ccttttgaac caatccttta cgccgtcaaa aaactcaaca ttttttccca
     5761 tttcaacaag gtcatttcgc aaaagaggca tattcctttc acgggacatt tttaccattg
     5821 cgtacatata cgcaagaatt gagtccatat gttcgctttg accgagttcg tttgaaaatt
     5881 tccagaactc gtcttcggtc atattaagac gggggataaa gctgtaatcc tgcatatcct
     5941 tcgtacatag ggtacggtca aagtcataca taatcgctgc aacgggcatt ctgttcataa
     6001 ccgctcctta tccgactgtg gtcggctgtg tgacgctttt ggcagatgtc gaatctgcct
     6061 gagttgttga ttcctcttta tattgtgtaa tcttaatgct tttgataaca atgttcttaa
     6121 cgggaatgtc gtccttgtca accttagctg aggcgatttt atcaacaaca tccattccgt
     6181 caattacctg tgcaaatacc gtgtagcctc tgtcaaccgc attgtatgca ccgtcaagat
     6241 aggcgtttcc gccgttttcc tcatacagct ttttaacgct gtcgggaacg ctgtcggtat
     6301 cgtaacagtt tgtgccgttt ttctcaatga atgcagtcag aaggtttgaa tccttgtagt
     6361 ttgcaagctg ggttttgata gaatcccatt gacttgcaag atgttcccag ccgccttcat
     6421 tcttatatgt ttcggcagtt ttctgattga tgaagaatgc actttcgttt gtgtccgcac
     6481 ctgtgtttga cattgcaaca gcgcctctga tattgaaaag cctgtcgcaa aattcatctt
     6541 caaatgcctc gccgtacgag cttgtaccgc agtaaccgcc gttgatcata aagtcttttg
     6601 ttactttgct gaaaagtgaa ttgttatact ttcctgcttt tgcaaggttt acaaagtttg
     6661 taacggtctt tggtgcctgt tcggggaaaa gtcgcattgt tatgtcaccg tatgaggtgt
     6721 gaacaactgc gattgtatcg ccgttcttcg gcatatcaag ctgaaaaccg acagggtttg
     6781 aatcgtgaat aatgttcgga ttgattccga gagtagcggt atcaatgccg atgctttcaa
     6841 gatagttctg tgcggtaggt tcatcagccg tgcttacggc agtagttgca ggtgttgttt
     6901 cgtcgggagt ctgcgagact tcagccttct tatcaccgca acccgaaaat gccaatgcag
     6961 aaacacacat aattgatgca accgatacgg cacacatttt tttaagattg taagtattca
     7021 tagatttgtt tccttcctgt caagcctcgt tgttatgtaa tgtttacaaa gtttccaaaa
     7081 acaacctcgg ctcttctaat ctggatttta ttctaaaaca cacacatttt ttgtaccata
     7141 atcacaaagt gcattatggt acaatcaccc atcgaaaatt ttgcatggca aaatttgagg
     7201 gggatataaa ttcgtataat aagcactcaa caaaagctca gatttcatcg tacttttcgt
     7261 gcttattata tcataaatga aataaaaatg caagtttgcc gctttttatg aaggaaagcg
     7321 tagcaaatgc aataaaattt ttctgcaaaa aattgcgtcc gtgtaataag acagaaaact
     7381 ttttcatatg attttctgta aacctaaata tgacggaggc tttaaaatgt cagaatattt
     7441 acccgaagga aaacttatat ccgaacagga aaacataaat atacttcact cattaaagat
     7501 gcttgaggat gcaaaaaaca gcgaaaagat tcttgaggca aaggcttgtg tgtgcgacag
     7561 cagtcacaac cttattgttg accttgcagg cataaaaggc ataattccga gggaggaggg
     7621 tgcaatcgga attaaggacg gctcggttcg tgacatagcc gtgatttcaa gggtgaaccg
     7681 tcccgtctgt ttcgttgtca ccgacataac cgaggacgaa aacggcaaac cgattgtaat
     7741 gctttcccgt gaaaaagcac aacgcaaatg ccttgacgca tacatttcaa ggttgcgatg
     7801 cggagatatt gtgcctgccc gcataactca tcttgaaaac ttcggtgcat tcgcagacat
     7861 aggctgcgga attgttgcac ttatgcccat tgacacaatt tcggtttcaa ggattgaaca
     7921 ccctgccgag agatttactg taggaatgga tatcaaagcc gttatcaaat ctgttgaaaa
     7981 cggtcgtata agcctcagtc acaaggagct tctcggcaca tgggaagaaa atgccgaaag
     8041 gttttgtgcc ggtgaaaccg tgtcgggaat tatccgaagc gtggaaaact acggtgcttt
     8101 tgtggagctt acaccaaatc ttgcaggact tgcagaaaca agagagggag tccgtgcagg
     8161 tcagcaggca agcgtttaca taaaaagtat cattcccgaa aaaatgaaaa tcaaactgat
     8221 aattattgac acctttgatt acaaatataa tccgaaatgc cccgaatact attgcgacag
     8281 cgaccgtatc gaccgttttg tatattcacc tgaaaattgt cggaaacggg ttgaaacaat
     8341 attcgatacc tgagttgaca gaaaaactcc cgaatgatat tatttaattg tgaaatcgaa
     8401 ttattgttcg ggaggtttaa tatgcagttt acgacatccg agattattgt gcttttattt
     8461 tgtgcaataa cgctcattgt cagcgttgtc acattaataa cagttctcaa aaagaataat
     8521 aacaacagaa cggagaacga acttcaaagg atattgctca atcagcaaag ctttgcacag
     8581 tcgcaaaatg acagggcgat tcagcttgaa caaagacttc acagcttttc agccgacaat
     8641 gttcagagcc ttgaaaatat cagacgctcg gttgatgaaa agcttgaaag tataaggcgg
     8701 gaaaatctcc gtcagcttga tgaaatgcga cagacagttg atgaaaagct tcaaaaaaca
     8761 cttgaagaaa aaatgaataa gtcattttcg cttgtcaacg aaagacttga acaggtttat
     8821 aagggactcg gcgaaatgca aacgcttgcg gtaggtgtgg gcgatttgaa aaaggttctt
     8881 tcaaatgtca aaacaagggg catactcggc gaaattcagc ttggtgcaat tctctccgaa
     8941 attcttacaa aggagcagta tgaggaaaat attgcaacaa aaaaaggctc aaaaaatgtt
     9001 gttgagtttg caatcaaact gcctgctgac ggtgccggta cagtatatct gcctgttgat
     9061 tccaaatttc cgggcgatac atattctgct ctccgtgatg cggttgaaag ctgtgacaag
     9121 caacgcattg aatcggcaca aaaagcactt gttcagcgta taaagggcga ggcaaaggat
     9181 attcacgata agtacattga tccgccgaac acaacggaat ttgcaattat gtttcttccg
     9241 tttgaggggc tttacagcga ggttgtcaat atgggacttg ttgaagtcct tcagcgtgag
     9301 tataaggtga atattgcagg tccgagtaca atggcggctc tgctcaattc gttgcaaatg
     9361 ggattcaaaa cccttgccgt gcaaaagaga agtgcagagg tttggaaaat actcggcggt
     9421 gtaaagaccg agttcgacaa gttcaatgat gtgcttgtaa tgacacagca aagacttgat
     9481 caggcaaaca aggagcttga caagcttgtg ggtgttcgca cccgtcaaat tcagcgtcag
     9541 cttaaagatg ttgaatcggt aaccctgtcg gaacagaatc tttttgaata aacaaaaaat
     9601 taaggcgaaa cagacacaaa tctgtttcgc ctttgttgtt attgcagatt aacctttgta
     9661 gtcaatccat tcgtcgtcaa cccattcttg ctttgtatcg ttccatcttc tgtactggag
     9721 ctgtccgtga cttccacgac gccatttctt gtaaattaca tcagctatcg gctgaacaga
     9781 agttgaatcg gcaccgctct ccaaagctga actgtcggga gttgctactt caacaactgc
     9841 aatgctgtcg ggggttgaat ctgtttctgc tgcatttgca accataacgg ttccttgtgc
     9901 gataattgct gtcataataa atgctgtaaa aattcttgcg gctttctttt tcatctgtta
     9961 tttctccctt aatataagac aataatcata gtagccgttt tctacatcgg agtatggaat
    10021 aataatagaa tcatcttttg tatagac
//
LOCUS       DAOMVE010000061         9750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1740_length_9750_cov_3.789170, whole genome shotgun sequence.
ACCESSION   DAOMVE010000061 DAOMVE010000000
VERSION     DAOMVE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9750
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1740_length_9750_cov_3.789170"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..368)
                     /locus_tag="PLS64_04830"
     CDS             complement(33..368)
                     /locus_tag="PLS64_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRL43187.1"
                     /translation="MQYFAFKVKVVIPKTVKVVDSFAFSFNDNIYELEIADGVKVLES
                     DAFTSMPNLKSVTVPDSVKEMDNCGLGMDVDDNYDSVPLKGFKMYCKKGSVAEAYAKE
                     NNLSYEIIK"
     gene            complement(373..1197)
                     /locus_tag="PLS64_04835"
     CDS             complement(373..1197)
                     /locus_tag="PLS64_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRL43188.1"
                     /translation="MGCTHDCSSCSSDCKSKEQNLHEELNKYSSVKKVIGIVSGKGGV
                     GKSLVTSMLAVTFNRLGKSTAVLDADITGPSIPKAFGVHERCRGNDEGIFPVVTETGI
                     KMISVNLLLEHETDPVVWRSPVITGTVKQFWKDVIWENVDYMFVDMPPGTGDVPLTVF
                     QSLPIDGIVIVASPQELVSMIVQKAVKMARMMNVPILGLVENMSWFMCPDCGKKHSIF
                     GDSRIEEVAKEYDTQVLAKLPIDPELAKCVDEGKIEFFEGNYLDEAAEIIEKKLSK"
     gene            complement(1245..2072)
                     /locus_tag="PLS64_04840"
     CDS             complement(1245..2072)
                     /locus_tag="PLS64_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43189.1"
                     /translation="MDDNNKYNRNKDYEPDNFDDEVAFPARRNNAPERSSDIQEDYGD
                     NFDNDVKFVGDYDEDAVQMPEIDFEDDMETPVKMPSERDDRTQTPDYGDNFDSQTGEQ
                     VSKATKDFQYDLSSDDIGNYYTKSTIHPKRKKVVKKKKRTSRNVAVILTIAAVICLIV
                     AVVFAFTQCSSQKNDNKASTAPSTEQVTTVQPTTEYGDDNYVPVYTEEPTYEPPTEYQ
                     PETEAPVQTEPQTEYQPETEPQQSSDISVTESSQTSDDTQPTQSYESGGDEPYVEEN"
     gene            complement(2285..2872)
                     /locus_tag="PLS64_04845"
     CDS             complement(2285..2872)
                     /locus_tag="PLS64_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43190.1"
                     /translation="MKKPTKRDTINLFFSAFLIIAFIVCAHFFSQYASALSQPLGSII
                     PILVYAVFGLLMFYATRVGDGKAVKRFSLVTLIVMVLPSLYIIVASIAPGLPLYDVFS
                     NANGSGLSVIVTLASIALGYGIPYSFLSGFEVADDEAVEEETGNPVQGGIEADLADDE
                     NAEDTAFEESEEVETEELSEDESESTDETDNGEDA"
     gene            3126..3545
                     /locus_tag="PLS64_04850"
     CDS             3126..3545
                     /locus_tag="PLS64_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL43191.1"
                     /translation="MKSMIGMNLKYLRKKYGYSQEDLAERLEVSRQSVAKWENEESLP
                     DVEKCVTMAQIFETTVEMLLVCPFYEEESDGLTPDGDGKYIFGIVKVSERGQITLPKH
                     ARKVFGIEAGDRLLVLGDEKKGMALAKIKNPFDKLMK"
     gene            3588..4316
                     /locus_tag="PLS64_04855"
     CDS             3588..4316
                     /locus_tag="PLS64_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL43192.1"
                     /translation="MYAIKTEKLTKKYKEKTAVSNLDLKINEGEMYGLLGVNGAGKTT
                     TMKMLTTLIMPTSGEAELLGHSIKTDRAAVKQIIGISPQESAAAMNLTARENLELMAE
                     VYGFSKKVAKAKASELIERFSMREFENRKAKKLSGGEVRRLSIAMALISEPKILFLDE
                     PTLGLDVIARHSLWVEIEKLKGKVTVILTTHYLEEAVALCDRIGIMAHGKFVSEGTSD
                     ELVALSGEKDFESAFVKLTNGGAL"
     gene            4316..5062
                     /locus_tag="PLS64_04860"
     CDS             4316..5062
                     /locus_tag="PLS64_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL43193.1"
                     /translation="MRLTAFASRNTKEILRDPLTFCFGLGFPVVLLLLLSAINSAIPE
                     QAQMTMFNIDKLTPGITVFGMSFISLFSSMLIAKDRTTSFMMRLFTSPLKAHEFIIGY
                     TLPLLPMAILQSAVCVVVAFFLGLEVSVNILLMLAVNIPIAIVFISLGLLLGTLLNEK
                     AVGGICGALLTNLSAWFSNIWFDTAMVGGWFKNLANALPFAHAVNAARDAISGNYSDI
                     MPELIPVCGYAVVLLVLAVVVFSVMMKRNK"
     gene            5066..5824
                     /locus_tag="PLS64_04865"
     CDS             5066..5824
                     /locus_tag="PLS64_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HRL43194.1"
                     /translation="MNPNGKALLKENKRYYLLDSLRGISTVSMVAFHLCYDIFMMYGL
                     NTSWYFYPMTAVWERSICVMFILLSGMCLNFSGNGIKRGVLLNLAGFAVTCVTVIAEP
                     NQAVWFGVLNLIGCSMMIVSVLSDNLKKISPLSGALISLLLYAVSYDLQKGCVGFFGL
                     DIVKLPDFLYSCKYLAFLGFPSSDFVSADYFPIIPWIFLFTAGFYLWNFIAQKNLAKY
                     FEFKIPLFDKIGKYSLWIYLAHQPVIMGVLMLIM"
     gene            complement(5893..6597)
                     /locus_tag="PLS64_04870"
     CDS             complement(5893..6597)
                     /locus_tag="PLS64_04870"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HRL43195.1"
                     /translation="MTREQKQHILHFAVSSLGGFMGGYAIYNHCDVFGNAQTANMIHL
                     VSKIFTGDFEGVVFIVIALLTYILGNVFFVISDRFIKLETKTVSLIASAVALLIIGIF
                     PNISNNYLAILPILFVTPVLWNSFRTAGSYVTSPIFSTNNLRVATVSTFTGIIDKDKD
                     MKKKAKFYWLTLASYHIGVVLACVPSVFIGVNSIWFGFVSIALSTASYMWLCGVFDRK
                     ESFAASEGLAAQSEII"
     gene            complement(6802..7731)
                     /gene="argF"
                     /locus_tag="PLS64_04875"
     CDS             complement(6802..7731)
                     /gene="argF"
                     /locus_tag="PLS64_04875"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRL43196.1"
                     /translation="MKHLLKLEDWSTEEIINTLNLADQLKYEQKHGIEHKILKGKSLG
                     MIFEKSSTRTRVSFEVGMTQLGGYPLFLSSNDLQIGRGEPVEDTARVLSRFLDGIMIR
                     TFEQKEVEALAEYGSIPIINGLTDYCHPCQVLADLMTIREFKGKLEGLKMCFIGDGNN
                     MMNSLIVGALSTGMSFSAACPKDYMPPKHIIEFGEKYGNGKFEIVTDPLEAAKNADVV
                     YTDVWASMGQEEEKKIREAAFAGYQVNKELMAVTNKDCMVLHCLPAHRGEEITADVFE
                     EHANEIFEEAENRLHAQKAVLVECMADEMPEKV"
     gene            complement(7731..8903)
                     /locus_tag="PLS64_04880"
     CDS             complement(7731..8903)
                     /locus_tag="PLS64_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HRL43197.1"
                     /translation="MTTKEKDLKYIMHTYGRYDVALKSAKGVTAYDENGKKYIDVSSG
                     IGVNSLGYCNDGWVEAVSKQAGTIQHMSNYFYCSQASDLAEKLCTLTGLSKVCFGNSG
                     AEANECAIKIARKYSFDKYGEGRNEIVTLNNSFHGRTVTTLAATGQDVFHNYFFPFTE
                     GFKYADADDMDALKAVVSDKTCAVMLELIQGEGGVNILDPEYVKELVKYCNDNDILVI
                     VDEVQTGVGRTGKLFAHQNYGILPDLMTVAKGLGGGLPIGVCMCGEKLKDVMSPSTHG
                     TTFGANPVVCAGANYVLDTVANDEFLAEVEKKGQYFEDKLTKIDGVKSVRRMGLMIGI
                     ELEKGDAHDIAIKCVENGLLIITAKDLLRMLPPLVIDYNEIDEAINILEKTIKENN"
     gene            complement(8919..9341)
                     /locus_tag="PLS64_04885"
     CDS             complement(8919..9341)
                     /locus_tag="PLS64_04885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL43198.1"
                     /translation="MIIREMTIDDYDEVYEMWQITTKRALSKADEKDQMERYLKHNAG
                     MSQVAVVDGKIVGTVLAGHDGRRGFIHHMAVLPEFRRKKIGHALAQTAIQKIREQGID
                     KTHIFCYQNNETGQSFWRDFGFEKREDVFVYSFSNDKI"
     assembly_gap    9549..9558
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         2697 a         2148 c         2023 g         2872 t
ORIGIN      
        1 tcggaaagct gtttttaatt tacattaact ttttacttga ttatctcata tgacaaattg
       61 ttttcttttg catatgcttc ggcgactgaa ccttttttgc aatacatttt aaagcccttg
      121 agcggaacac tgtcataatt gtcatcaaca tccataccaa gaccgcaatt atccatttcc
      181 tttacggaat cgggaacagt aacgctttta aggtttggca tagatgtgaa cgcatcgctt
      241 tcaagaacct ttacaccgtc ggcaatttca agctcgtata tattgtcatt gaagctgaat
      301 gcaaacgaat ccacaacctt aacggttttc gggataacca ctttaacctt aaaagcaaaa
      361 tattgcaaat acttatttgc taagtttttt ttcgattatt tctgccgctt cgtcaagata
      421 gtttccttca aaaaattcaa tctttccctc gtcaacacat tttgcaagtt cgggatcaat
      481 cggaagcttt gcaagcacct gtgtgtcata ttcctttgca acttcctcaa tgcggctgtc
      541 gccgaagatg ctgtgtttct ttccgcaatc gggacacatg aaccagctca tattttcaac
      601 aagacctaaa atcggcacat tcatcatcct tgccatttta acggcctttt gaactatcat
      661 tgaaaccagc tcctgcggtg acgcaacaat aacaatgccg tcaatcggca ggctctgaaa
      721 taccgtaagc ggaacatcgc ctgttccggg aggcatatca acaaacatat agtcaacatt
      781 ttcccagatt acatctttcc aaaactgctt taccgtgcct gtgataaccg gcgaacgcca
      841 caccacggga tcggtttcat gttcaagaag aaggtttacg gaaatcatct tgattcccgt
      901 ttcggtaaca acggggaaaa ttccctcatc gttgcctctg catctctcat gtactccgaa
      961 tgcctttgga attgacggac ctgtgatatc tgcgtcaaga acagccgtgc ttttaccgag
     1021 tcggttaaat gttactgcga gcattgaggt tacaagactc ttgcctactc cgcccttgcc
     1081 cgacacaatg ccgattacct tttttacact gctgtattta ttaagttctt cgtgcaaatt
     1141 ctgttccttg cttttgcaat ccgaagaaca gcttgaacaa tcgtgggtgc aacccattta
     1201 aaacatctcc aatttcataa attcatttta tcaaaagaaa acaatcagtt ttcttcaaca
     1261 tacggttcat cgccgccgct ctcgtatgac tgtgtcggct gagtgtcatc cgaagtctgt
     1321 gacgattcgg taacactgat atcactgctc tgctgcggct ctgtttcagg ctgatactct
     1381 gtctgcggtt cagtttgaac aggcgcttct gtttcaggct gatattcagt cggcggttca
     1441 tatgtcggtt cttccgtgta aacgggaaca tagttgtcat cgccgtattc cgttgtaggc
     1501 tgtaccgttg tcacctgttc cgttgacggt gctgtggaag ccttgttatc atttttctgt
     1561 gaactgcatt gtgtgaatgc aaaaacaacg gcaacaataa ggcagattac cgccgcaatt
     1621 gttaagataa ccgccacatt acggcttgtt ctctttttct ttttaacaac ctttttacgc
     1681 tttgggtgta tcgttgattt tgtgtagtag ttaccaatat cgtcagatga aaggtcatac
     1741 tgaaaatcct ttgtagcctt tgacacctgc tcccctgtct gagaatcaaa attatcgcca
     1801 tagtcgggag tctgtgtgcg gtcatctctt tccgacggca tttttacggg agtttccata
     1861 tcgtcctcaa agtcgatttc cggcatctga acggcgtctt cgtcataatc gccgacaaat
     1921 tttacatcat tgtcaaagtt atcgccgtaa tcctcctgaa tatcggaaga tctttcgggg
     1981 gcgttgtttc ttcttgcagg aaacgcaacc tcgtcatcaa agttgtcagg ctcataatcc
     2041 ttgtttctat tatatttatt gttatcgtcc attatgtaat tctcctttga aagaaaaatt
     2101 ttgaaacaga aaaatacggt gcatttattc tgcacacccc gtaccaaaat aataaatcag
     2161 ccgtgacaat ttgtcaaccg aatattagga ctgttaagtt taaaagcata atttttgcca
     2221 tatacaaaaa gtacccgcac aagccgtgca ggtactcgtc gatttaagaa aaatcagcag
     2281 tgaatcaagc gtcttcaccg ttatcggttt cgtctgttga ttcgctttca tcctcggaaa
     2341 gttcctctgt ttcaacctct tcggattctt caaatgcagt atcttctgcg ttttcgtcat
     2401 ctgcaagatc agcctcaatg cctccctgaa cagggttgcc tgtttcttct tcaacagcct
     2461 catcatcggc aacttcaaaa ccgctgagga atgagtaagg aattccgtaa ccgagtgcga
     2521 tacttgcaag cgttacaata acgcttaatc ccgagccgtt tgcatttgaa aatacatcat
     2581 aaagcggaag tcccggagca atggaagcaa cgattatgta gagtgacggg agcaccatta
     2641 caataagtgt aacgagcgaa aaacgcttta ccgccttgcc gtcaccgaca cgggttgcat
     2701 agaacatcaa aagaccgaac acagcataca caagaatcgg aatgattgaa ccgagtggct
     2761 gtgaaagggc tgatgcgtac tgtgagaaga agtgtgcgca aacgataaat gcgatgatca
     2821 gaaatgcaga gaaaaacaga ttgattgtat ctcttttagt aggttttttc ataatatgtc
     2881 ctccgtacat acgatattgg tttaagttta acattgaaat gtgacagttt tgtgaacaaa
     2941 caatgatatg tacattagaa taatgtaaac aaggcgtaaa aaacgcaacc gccttttgtt
     3001 tgacaaaatc tgtaagggag tttatcataa atcataaagg gattatgcaa ctgattttta
     3061 cataaagtgt aaaatcaggt tggtagaaat tgcgttacat atgaaatata cttaactcag
     3121 gaggtatgaa aagtatgatt ggtatgaatt tgaagtatct tcggaaaaag tacggatatt
     3181 cacaggagga ccttgcggag aggcttgagg tgtcaagaca gtctgttgca aagtgggaaa
     3241 acgaggaatc tcttcccgat gtcgaaaaat gcgtgacgat ggcgcagatt tttgaaacta
     3301 cggtagaaat gcttcttgtg tgtccttttt acgaggaaga atctgacgga ctcactcccg
     3361 acggtgacgg aaagtacatt ttcggcattg ttaaggtcag cgaaagagga cagattacac
     3421 tccctaaaca cgccagaaag gtgttcggaa ttgaggcagg cgacagactt ctggttctcg
     3481 gtgacgagaa aaaaggtatg gcgcttgcaa aaataaaaaa tccctttgat aagttaatga
     3541 agtaaaattt gacttgaatc ccttttatcc tgaattgagg agaaaatatg tacgcaataa
     3601 aaacagaaaa acttacaaaa aaatataagg aaaaaacggc tgtatcaaac cttgacctta
     3661 aaatcaacga gggcgaaatg tacggtttgc tcggagtcaa cggtgcagga aaaacaacaa
     3721 caatgaaaat gctcacaacg cttattatgc cgacatcggg tgaggcagag cttctcggtc
     3781 acagcataaa aaccgacaga gcagcggtaa aacagattat cggaatttca ccgcaggaaa
     3841 gtgcggcggc aatgaacctt accgcaagag aaaatcttga actcatggca gaggtttacg
     3901 gcttttcaaa gaaagttgca aaagccaaag caagcgagct tatcgaacgc ttttccatgc
     3961 gggaatttga aaaccgcaag gctaaaaagc tgtcgggcgg tgaggtcaga agactgagca
     4021 ttgcaatggc acttatctca gaaccgaaaa ttctctttct tgacgaaccc acgctcggac
     4081 ttgatgtaat tgcaagacac agcctttggg ttgagattga aaagctcaag ggtaaagtca
     4141 ccgttattct cacaacgcac tatctcgaag aggctgttgc actctgcgac agaatcggta
     4201 ttatggctca cggtaaattt gtgtctgagg gtacttccga tgagcttgtt gccctttcgg
     4261 gcgaaaaaga ttttgagtcg gcttttgtaa agctgacaaa cggaggtgcg ttgtaatgag
     4321 attgactgcg tttgcttcac gaaatacaaa ggaaattctc cgtgatccgc tgacattctg
     4381 tttcggactc ggatttcccg ttgtacttct tcttttgttg tcggcaataa attcggcaat
     4441 ccccgaacag gctcagatga caatgttcaa catcgacaag ctgacgcccg gtattacggt
     4501 tttcggaatg agctttatct cgctgttctc gtcaatgctc attgcaaagg acagaactac
     4561 aagctttatg atgagattgt tcacatctcc gctcaaagca cacgagttta ttatcggcta
     4621 cacacttccg cttttgccaa tggcaatttt gcagagtgcc gtatgcgttg ttgttgcgtt
     4681 cttcctcgga cttgaggtca gcgtgaacat tttgcttatg cttgcggtaa acattccgat
     4741 tgcaattgtg tttatctcgc tcggacttct gctcggcaca cttctgaatg aaaaggctgt
     4801 cggcggaatc tgcggtgcat tgctcacaaa cctttcggca tggttttcaa acatttggtt
     4861 cgatactgca atggtcggcg gttggttcaa aaaccttgca aacgcacttc cgtttgccca
     4921 tgccgtaaac gccgcaaggg atgcaatttc gggcaactat tcggacatta tgcccgaact
     4981 tatccccgtc tgcggatatg cggttgtgtt gcttgttctg gctgtggtcg tcttttccgt
     5041 tatgatgaaa aggaataaat aattgatgaa tccaaacggt aaagccttgt taaaagaaaa
     5101 caaaagatat tatcttctcg actccctgcg tggaatttca accgtcagta tggttgcgtt
     5161 tcacttatgc tacgatattt ttatgatgta cggacttaac acaagctggt acttttatcc
     5221 catgactgcg gtctgggaac gcagtatctg cgtaatgttc atactgcttt cgggaatgtg
     5281 ccttaacttt tcgggaaacg gcataaaaag aggtgttctt ttaaatcttg cgggctttgc
     5341 cgttacctgc gttaccgtga tagccgaacc gaatcaggcg gtatggttcg gtgtactgaa
     5401 tctgataggc tgttcaatga tgattgtttc cgttctgtcg gataacctca aaaaaataag
     5461 ccccctgtcg ggagcgttga tttcactttt gttgtatgcg gtttcgtatg atttgcaaaa
     5521 aggctgtgtc ggatttttcg ggctggatat cgtaaaattg cccgattttc tctattcgtg
     5581 caagtacctt gcatttctgg gattcccctc gtccgacttt gtgtcggcag actatttccc
     5641 gattattccg tggattttcc tctttacggc aggcttttat ctttggaatt tcatcgcaca
     5701 aaaaaatctt gcaaaatatt ttgaattcaa aattccgctg tttgacaaaa tcggcaaata
     5761 cagcctgtgg atttatcttg cccaccagcc tgttattatg ggtgttttga tgttgataat
     5821 gtgactttgc cgattgaaag ttataaacaa aaaaataatg tcggaggtgt accgccgaca
     5881 ttatttttta ttttaaatta tttccgattg tgctgctaag ccttcggatg ctgcaaagga
     5941 ttcttttctg tcaaacacac cgcagagcca catatatgaa gctgttgaaa gggcaatgct
     6001 tacaaagccg aaccagatgc tgttcacacc gataaaaacg ctcggaacgc aggcaaggac
     6061 aacacctata tgatagcttg caagcgtaag ccaatagaat ttagcctttt tcttcatatc
     6121 cttgtccttg tcaataatac ccgtgaatgt tgaaaccgtt gcaacacgaa ggttgtttgt
     6181 cgagaaaatc ggagatgtaa cataacttcc ggctgttctg aatgaattcc acaaaacagg
     6241 agttacgaac agaatcggca gaattgcaag gtagttgttt gaaatattcg gaaaaattcc
     6301 gataatcaga agtgcaaccg cacttgcaat aaggctgacg gttttggttt caagcttaat
     6361 aaaacggtcg gaaattacaa agaaaacatt gccgagaata tatgtaagga gagcgatgac
     6421 gataaacaca acgccctcaa aatcacccgt aaaaattttg ctgacaaggt gaatcatatt
     6481 tgctgtctgt gcgttaccga acacatcaca gtgattgtaa attgcatatc cgcccataaa
     6541 tccgcccaga gagctgactg caaaatgcag aatatgctgt ttttgttctc ttgtcatttt
     6601 aaagacttcc tttttcgcca taaaacgtgt aattgcttaa cataattata cacctgcaat
     6661 tctgattttg ctattatccc ccaatggaat tatactccgt tttgcaataa aaattaagtc
     6721 acattgcaca aaacagctgt cttactgttg tacaatgtga cttttttgct ttttatgaag
     6781 ttttaaagaa tcaaactgtc atcaaacttt ttcaggcatt tcatctgcca tacattcaac
     6841 aagaacagct ttctgagcgt gaagacggtt ttctgcctcc tcaaagattt catttgcgtg
     6901 ttcttcaaat acatctgcag taatctcttc gccacggtgt gcagggagac aatggagaac
     6961 catacaatcc ttgtttgtaa cagccataag ctccttgtta acctgatagc ctgcgaatgc
     7021 tgcctcacgg attttctttt cttcttcctg tcccattgat gcccatacat ctgtataaac
     7081 aacatcggca ttcttagcgg cctcaagcgg atctgttacg atttcaaact tgccgttacc
     7141 gtacttttca ccgaactcga taatatgctt aggcggcata taatcctttg gacaggcggc
     7201 agagaagctc atgcctgtgc tgagtgcgcc gacgatgagc gagttcatca tgttgttgcc
     7261 gtcaccgata aagcacatct tgagtccttc aagcttgccc ttgaattcac gaattgtcat
     7321 aaggtctgcg agaacctgac atggatgaca atagtctgta agaccgttga taatcgggat
     7381 tgaaccgtac tctgcaagag cctcaacttc tttctgctca aaggtacgaa tcataatacc
     7441 gtcaagaaaa cgggaaagaa cacgggctgt gtcctcaaca ggctcgcctc tgccaatctg
     7501 aaggtcattt gatgagagga aaagcggata accgccgagc tgtgtcatac caacctcaaa
     7561 tgaaacacgg gtacgggtgc ttgacttttc aaaaatcata ccaagagact tgcccttgag
     7621 aattttatgc tcaatgccgt gtttctgctc atatttaagc tgatcggcaa ggttaagtgt
     7681 gttgataatt tcttctgttg accaatcctc aagtttaagt aagtgtttca ttagttgttc
     7741 tcctttattg ttttttcgag tatattaatc gcctcgtcaa tttcattgta atcaataaca
     7801 agcggtggga gcatacggag caaatctttt gccgtgataa taagcaagcc gttttcaacg
     7861 catttgattg caatgtcgtg tgcatcgccc ttttcaagct caataccaat cataagtccc
     7921 attctgcgaa cgctctttac gccgtcaatt tttgtgagct tgtcctcaaa atactgacct
     7981 tttttctcaa cctcggcaag aaattcgtca tttgcaactg tgtcaagaac atagtttgca
     8041 cctgcacaaa caacagggtt tgcgccgaat gttgtgccgt gagttgacgg tgacataaca
     8101 tccttgagct tttcaccgca catacaaaca ccgataggaa gtccgccgcc gagccccttt
     8161 gcaaccgtca taaggtcggg gaggataccg taattctgat gagcaaacag cttgcctgtt
     8221 ctgcctacgc ctgtctgaac ctcatcgaca ataacgagaa tatcgttgtc gttgcaatac
     8281 ttaacaagtt cttttacata ttcgggatcg aggatattta caccgccctc gccctgaatg
     8341 agttcaagca ttactgcgca ggttttgtct gaaacaactg ctttcagagc gtccatatcg
     8401 tctgcgtctg catacttaaa gccctctgta aacgggaaga aatagttatg aaaaacatcc
     8461 tgacctgttg cggcaagagt tgtaacagtt ctgccgtgaa aggaattgtt aagcgttacg
     8521 atttcatttc tgccctcgcc gtacttgtca aagctgtact tacgggcaat tttgattgcg
     8581 cattcgttag cctctgcacc gctgttgcca aagcagacct ttgaaagacc tgtaagtgtg
     8641 caaagctttt cggcaaggtc acttgcctgt gaacagtaaa aatagtttga catatgctga
     8701 attgtgcctg cctgctttga aacagcctca acccaaccgt cattgcaata gccaaggctg
     8761 tttacaccga tacccgatga aacatcaatg tactttttgc cgttttcgtc atatgcggta
     8821 acgccttttg cacttttgag agcaacatcg tagcgaccgt atgtgtgcat aatatatttt
     8881 aaatctttct cttttgtagt caagttagcc acaccctttc atattttatc gttgctgaag
     8941 gagtaaacaa aaacatcctc acgcttttca aagccgaaat cacgccagaa gctttgacct
     9001 gtttcgttgt tctgataaca gaaaatgtga gttttgtcga tgccttgttc acggattttt
     9061 tggatagcgg tctgtgcaag agcgtgaccg attttctttc gtctgaattc gggcagaaca
     9121 gccatatggt ggataaatcc ccgtctgccg tcatgacctg caagcacggt tccgacaatt
     9181 tttccgtcaa ccacggcaac ctgactcata ccggcgttgt gcttgagata acgctccatc
     9241 tgatcttttt catcagcctt agagagcgca cgctttgtgg taatctgcca catctcatag
     9301 acctcgtcat agtcgtcaat tgtcatttct cgtataatca ttgcaccctc caaaatctaa
     9361 tctgtagggg cggatatcat ccgcccgcac ggcgagtcgc cgtcccccgt tcgtgtagac
     9421 agtttgataa attagaaact atccataccc gagcgtagca tagctaccgt ttgcacaatt
     9481 ctgtaggggc ggatatcatc cgcccgcaag aatagaagaa atatttttaa tgtactattt
     9541 ttataaaann nnnnnnnnag gagcacatgg tgctccccta caaacaatac gctgctttta
     9601 tcaaataaaa attttaagtt aaacagaaag ttttatttag cgagcgacca atgaccgcct
     9661 ctactgtttt tacaaatcag caaaacagca atgctaatca ataaaacatt gtaccgatgc
     9721 cttcatcgct gaaaagctcg agcagaattg
//
LOCUS       DAOMVE010000062         9396 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1838_length_9396_cov_3.803233, whole genome shotgun sequence.
ACCESSION   DAOMVE010000062 DAOMVE010000000
VERSION     DAOMVE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9396
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1838_length_9396_cov_3.803233"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..317)
                     /locus_tag="PLS64_04890"
     CDS             complement(120..317)
                     /locus_tag="PLS64_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HRL43199.1"
                     /translation="MSSNNNQINVPQAKEAMERFKMECANEVGVNLKQGYNGDISARE
                     AGSVGGQMVKKMIESYENSMK"
     gene            complement(584..3013)
                     /locus_tag="PLS64_04895"
     CDS             complement(584..3013)
                     /locus_tag="PLS64_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43200.1"
                     /translation="MKKTNKNNNHKFLSKNYIKGAISGALALTILCSGVGVAAYSAGA
                     DNTAQALGVQTESTVEAVKKASTTKEALKKLSKNETVYVIADASGAAKKIIVSDWLKG
                     VDTKGKVKDVSNLKDVKNVKGDETYTVNEDNAYEWAANGDDIYYQGTGTTELPVKLKL
                     SYKLNGKTVSADEIAGKSGKVTIRIDYENTQKEKVKINGKTEEVNVPFLMLSGMILDD
                     DNFKNVEVSNGKAINDGTRTIVAGFALPGMQDSLDIDKDEMEIPDYVEITADTTDFEL
                     STTMTVAMNDIFNDVDFSKVDDKVDELKDSLDELEDAAEQLVDGSSQLYDGIGTLLDK
                     SGTLIEGIDKLYDGAEQVNSGAKKLDSGADGLSSGAKTLDDGASKLKNGASQLDSGAS
                     QLDSGLATLAGGASQVDNGAASLSSYVATLAGGLGKLSSNSATLNGGAEQVFNTLLST
                     ADTQIAAAGLTADKLTIKNYDSVLEGLISSLSDENAQKLAYNTALETVTATVNSQKDV
                     IRTAVEATVRKSVTEQVLAAAGLGMTADQYDAAVAAGQVSEEVQAQVSTAVSTQMSSS
                     AVQTAIDSNTEAQIQSLIETNMNSEEVQSQIQAGVAKAQAGRKSLQALKTQLDSYNTF
                     YQGVLSYTAGVDQANEGAQQILAGTYTLKDGTGSLVSGAGQLKNGSSSLKSGTSELKS
                     GTSTLKDGTSSLKSGVKTLKNGTSSLSDGTKSLFEGVGTLKDGSAALVDGVKQLNDGA
                     MTLSKGMKKFKEEGIDSIVDAANGDVQDIIDRFKAVQKASKKYNNYSGISDDMDGKVD
                     FIYKTDSIEKK"
     gene            complement(3013..5106)
                     /locus_tag="PLS64_04900"
     CDS             complement(3013..5106)
                     /locus_tag="PLS64_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HRL43201.1"
                     /translation="MKLGKAVVKSRFVILILAVALMIPSALGMAFTRVNYDILSYLPD
                     NLDTIKGQDYLLDDFGKGAFSFLIFENMDDKDVAATEEKIKEIDHVDTVLWYDDFADI
                     SIPKEMLPDKIYDAFNSGNATMMAVFFDTSTSADETMNAIEEIRSVAGKQCFVSGMSA
                     MITDMKDMFEEQEMTYVAIAVVLAVIAMMIFMDNWIIPFVFLASIAVAILVNMGSNYF
                     MGEVSFITKALAAVLQLAVTMDYSIFLWHSYEEQKGICENHKQAMAVAIHETVRSVIG
                     SSITTVAGFIALCFMTFTLGLDLGIVMAKGVILGVISCVTVLPSLILVLDKPLEKTMH
                     KSLIPSTKKLATGLVKIFPVFLVMFAALIYPFYSAYSQTNNEIYYDLSESLPEDMANV
                     IANTKLKDEFGVGTTHMVLVDSSLSAKDARNMTNELEKVDGVKYVLGLESVVGSLVPE
                     EVLPDSITSVLKNDKWELMLINSEYKTATDPMAKQIDELNKIITKYDKDGLLIGEAAC
                     TNDLVNITTVDFQVVNAISIVAIFIIIAIVEKSITLPIILVAVIELAIFINLGIPHLM
                     GESLPFITPICISTIQLGATVDYAILMTTRYKKERYLGNDKRTSVITALETSIPSIIV
                     SAMGLFAATIGVAFYSDVDLISSICKLLARGAVISMFCVTLILPAMFMLFDKVIAKTT
                     LGFKPKKTSISEAAV"
     gene            complement(5366..5932)
                     /locus_tag="PLS64_04905"
     CDS             complement(5366..5932)
                     /locus_tag="PLS64_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL43202.1"
                     /translation="MPTFTKAAIKATFISLLNKKPLNKITVKEIVEECGINRNSFYYH
                     FDDIPSLLEEILVEEADRFVATETDNNTSVYESLISVIDYAFSNKSVIQHIYNSANRT
                     TFDVYLNRICTHAIKSYFDKLEITKNIAEDDLDAMIMYYKCQLVGFIIDWLGGGMKYD
                     LRIKMKRICELFEGSMESALDRCAKINA"
     gene            complement(6207..6803)
                     /locus_tag="PLS64_04910"
     CDS             complement(6207..6803)
                     /locus_tag="PLS64_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HRL43203.1"
                     /translation="MKTRLFITDIRKISFDRVGELSPERAERVQRCRRADDKLRCIAG
                     GLFMNKFLGGAKITVNEFGKPECDNGLCFNISHSGSYVLFALSDSEVGCDIEEIRFLK
                     GIRLGKIVFCDNEMKLLGNAFDRLGTFFELWTKKEALLKCMGDGFHRGAKSVDVSSGK
                     FSENQTEYYMKQYKFSDYEISLCSVQNDFPKDIEFIIL"
     gene            complement(6805..7293)
                     /locus_tag="PLS64_04915"
     CDS             complement(6805..7293)
                     /locus_tag="PLS64_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRL43204.1"
                     /translation="MDRIDKINYYLDIAETVLERGTCLRRNFGAIIVNNDQIVSTGYT
                     GAPRGRKNCCDLGFCIRQERNIPRGERYELCRSVHAEANAIIHASREQMIGATLYLVG
                     KEMSNGEYVQNTSSCAMCKRMVINAGIDKVIVRNNKTEFTEIDVNDWIMSDDSLDALR
                     GY"
     gene            complement(7363..7599)
                     /locus_tag="PLS64_04920"
     CDS             complement(7363..7599)
                     /locus_tag="PLS64_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRL43205.1"
                     /translation="MIYTNFREIDKAGRIVISKDIRNHLNIKPGDILHIEADDECVTI
                     KKAESKCVFCNTLDNLIAFGGKYVCRDCVEKLKG"
     gene            7760..8344
                     /locus_tag="PLS64_04925"
     CDS             7760..8344
                     /locus_tag="PLS64_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HRL43206.1"
                     /translation="MLNYIWSGLIAVSVLCALFMGNTADISNALIESGASSIQLILTM
                     AGIICLWSGIMKIAVESGLTNIFAKIFAPLLRPLFPRLDKKSDAFQSITMNISANLLG
                     LGNAATPFGLKAMEQLHTLNDRRDIASNEMVVFVVMNTASLQLLPTTLAALRQSYGSS
                     APFEIIVPVWISSACALTVALLIACTLNIRQGKI"
     gene            8361..8873
                     /locus_tag="PLS64_04930"
     CDS             8361..8873
                     /locus_tag="PLS64_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HRL43207.1"
                     /translation="MEFVMPAFACIVVVFGLIKKVPVFDVFISGAKEGVKLLYNIAPT
                     IMGLVFAVDLLQSSGAIDAICALIQPTAEFLGFPKEIVPLVLLRPVSGSGSTALLTAL
                     YKTCGPDSFAGRTASVLAGSSETTFYAIAMYFGCIRVKKIRHTLFAAITADLTAAIMS
                     VITVRLWFNM"
     gene            complement(9077..>9396)
                     /locus_tag="PLS64_04935"
     CDS             complement(9077..>9396)
                     /locus_tag="PLS64_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43208.1"
                     /translation="IQNHTGIDFLKDSIILAAPQINQDAVRLMKELYGIDMEKKQYSK
                     LISSIPEPDISISMGCDVGCPFIGRAFDDNWKLEDPTGKTDDDFRGVIQQIEKNILEL
                     KKK"
BASE COUNT         2584 a         2128 c         1839 g         2845 t
ORIGIN      
        1 actgtgcgaa tgtgcagtcc tgttttttat gttccgaatc gtccataata agcctgtgag
       61 cgaaaaatag gggcatacag ctaacatagc tatatgcccc tgaaaatcgg ttttaaagct
      121 tatttcatgc tgttttcgta agactcaatc atctttttaa ccatctgacc gcctacagaa
      181 cctgcttctc ttgcagaaat gtcgccgtta tatccctgtt tgaggtttac gcctacttcg
      241 ttggcacatt ccatcttaaa gcgttccatg gcttcctttg cctgaggtac attaatctga
      301 ttgttattgc ttgacataat ttgttttctc cttttcaaaa cttttaaatc tcgttaaatt
      361 tattaataaa gcggcgtttc ggcatcccgt tttcatcgcc gcaatagtat tgtttgcggt
      421 gaaattcgtt ttattatttg taattttttc aagttttgaa attcacaaaa aaacatcata
      481 caaagcaaaa agcagctatg ccaaacgaca taactgcttt ttatattgtg tatgaaaggg
      541 ttagggaaat aagcctttcg tcttgctgtt aaaatatatt gagttacttt ttctcgattg
      601 aatcggtctt gtagatgaag tcaaccttac cgtccatatc atcggaaata ccgctgtagt
      661 tgttgtactt ctttgatgct ttctgaacag ccttgaaacg gtcgatgata tcctggacat
      721 caccgtttgc ggcatcaaca atgctgtcaa tgccctcctc tttgaacttc ttcataccct
      781 tggacaatgt cattgcaccg tcattgagct gtttaacgcc gtcaacaagt gctgcactgc
      841 cgtccttgag agtaccaacg ccctcgaaga ggctctttgt accgtccgaa agtgagcttg
      901 tgccgttttt gagagtcttt acaccgcttt taaggcttga tgtaccgtcc ttgagggttg
      961 aagtaccgct cttgagttcg cttgtaccgc tcttaagact tgaagaaccg ttcttgagct
     1021 gaccggcacc gcttacaagt gaacctgtgc cgtccttgag tgtatatgtg ccggcgagaa
     1081 tctgctgagc gccctcgtta gcctgatcaa cacccgctgt gtatgaaagc acaccctgat
     1141 agaatgtgtt gtagctgtca agctgagttt tgagagcctg aagtgacttt ctgcctgcct
     1201 gagcttttgc aacacctgcc tgaatctgac tctgaacctc ttcgctgttc atatttgttt
     1261 cgataaggct ctgaatctgt gcctctgtat tgctgtcaat tgcagtctga accgcagatg
     1321 aactcatttg tgtagaaact gcagtagaaa cctgagcctg aacttcttcg ctgacctgac
     1381 ctgcggcaac agcggcatca tactgatctg ccgtcatacc aagacctgcg gctgcgagaa
     1441 cctgttccgt aacagacttt cttactgttg cctcaacggc agttctgata acatccttct
     1501 ggctgtttac agttgccgtt actgtttcaa gtgctgtatt gtaagcgagc ttctgagcat
     1561 tttcatcgct gagtgatgaa ataagtcctt caagaacgct gtcatagttt ttaattgtaa
     1621 gcttgtcggc tgtaagacct gcggcggcaa tctgagtgtc ggcagttgaa agaagtgtgt
     1681 taaacacctg ttctgcaccg ccgtttagag ttgcgctgtt ggatgaaagc ttgccgagac
     1741 cgcccgcaag tgttgcaaca taactgctga gacttgccgc accgttgtca acctgtgacg
     1801 caccgcctgc aagtgttgca agaccgctgt caagctgtga tgcaccgctg tcaagctgtg
     1861 acgcaccgtt tttgagcttt gacgcaccgt catcaagcgt ttttgcaccg cttgaaagac
     1921 cgtctgcacc gctgtcaagt ttttttgcac cgctgtttac ctgttctgca ccgtcataaa
     1981 gcttgtctat accttcaatg agtgtgcctg acttgtcaag aagtgtgccg ataccgtcat
     2041 aaagctgtga agaaccgtca acaagctgtt ctgcggcgtc ttcgagttca tcaagagaat
     2101 ccttaagctc gtcaacctta tcgtcaacct ttgaaaaatc aacatcgttg aaaatatcgt
     2161 tcattgcaac tgtcattgtt gttgaaagtt caaaatcggt tgtatctgct gtgatttcaa
     2221 cataatcagg gatttccatt tcatccttgt caatatcaag gctgtcctgc atacccggaa
     2281 gagcaaaacc ggcaacgatt gttcttgtac cgtcattgat agccttgccg tttgaaacct
     2341 caacattttt gaagttgtca tcgtcaagaa tcatacccga aagcataagg aacggaacat
     2401 taacttcttc tgtcttgccg ttaatcttaa ccttttcctt ctgtgtgttt tcatagtcaa
     2461 tgcggattgt aaccttaccg ctcttgcctg caatttcatc ggcagaaaca gttttgccgt
     2521 tgagcttata gcttaatttg agctttacgg gaagttcggt tgtgcctgtt ccctgatagt
     2581 agatatcatc gccatttgca gcccactcat aagcgttgtc ttcatttacc gtgtatgttt
     2641 cgtcaccctt aacattttta acatccttga ggtttgaaac atcctttacc ttgccttttg
     2701 tatctacacc cttgagccag tcgcttacaa tgattttctt agccgcaccg cttgcgtcgg
     2761 caataacata aacggtttca tttttgctga gctttttgag agcctcttta gttgtgcttg
     2821 ccttcttaac agcctcaacc gtactttctg tctgaacacc gagagcctgt gcagtattgt
     2881 ctgcaccggc ggaataagcg gcaacgccta ctccgctgca aagaattgtg agtgcaagcg
     2941 cacctgaaat agcacccttg atatagtttt tgcttaaaaa tttatgatta ttatttttgt
     3001 tagtcttttt cattatacag cagcctccga aattgatgtt tttttcggct taaaaccgag
     3061 agttgtcttt gcaattacct tgtcgaacag cataaacatt gccggcaaaa taagcgttac
     3121 gcagaacata ctgatgacag cgcctcttgc aaggagctta cagattgatg aaataaggtc
     3181 aacatccgag tagaatgcga cgccgattgt agcggcaaag agtcccattg cactaacgat
     3241 aattgacgga attgaagttt caagagccgt aataaccgag gtgcgtttat cgtttccgag
     3301 atacctttcc ttcttatatc gtgtcgtcat caaaattgcg tagtcaaccg ttgcgccaag
     3361 ctgaattgta cttatacaga taggtgtgat gaacgggagc gactcgccca taagatgcgg
     3421 aataccgagg ttaataaaga ttgcaagctc aataaccgca acaagaataa tcggaagtgt
     3481 gatactcttt tcaacaatcg caatgataat aaagattgca acaatagaaa ttgcatttac
     3541 aacctgaaag tcaactgtcg tgatgtttac aaggtcgttt gtacaagcgg cttcaccgat
     3601 gagaagtccg tccttatcat actttgtaat gattttattc aactcgtcaa tctgctttgc
     3661 cataggatct gtggctgttt tgtactcaga gttgataagc ataagctccc atttatcatt
     3721 tttgagaacg cttgtgattg aatcgggaag aacctcttcc ggaacaagcg aacctacaac
     3781 gctttcaagg ccgagaacat attttacgcc gtcaaccttt tcaagctcgt ttgtcatatt
     3841 tcttgcgtcc tttgcagaaa gcgaagaatc aacaagaacc atatgtgttg taccaacacc
     3901 gaattcatct ttaagctttg tgttggcaat tacatttgcc atatcttcgg gaagactttc
     3961 cgacaaatcg tagtaaatct cattgttcgt ctggctgtat gccgaataga acggataaat
     4021 aagagccgca aacattacga gaaatacggg gaaaatttta acaagtcctg ttgcaagctt
     4081 ctttgttgac ggaataagcg acttatgcat tgtcttttca agaggcttgt caagaacaag
     4141 aatgagtgac ggaagaaccg ttacacagga gataacaccg aggataacac cctttgccat
     4201 tacaataccg aggtcaagac cgagtgtgaa tgtcataaag caaagtgcga taaagcctgc
     4261 aaccgttgtg attgaacttc cgattacgga acggacggtt tcgtgaattg caacagccat
     4321 tgcctgcttg tggttttcac aaataccctt ttgttcttcg tagctgtgcc acaggaagat
     4381 tgagtagtcc attgttaccg caagctgaag aactgccgcc aacgctttgg taatgaatga
     4441 aacctcaccc ataaagtagt tactgcccat atttacgagg atagcaaccg ctatacttgc
     4501 aaggaacaca aacgggataa tccagttatc cataaagatc atcattgcaa ttaccgcaag
     4561 gacaacagcg attgcaacat aagtcatttc ctgttcctca aacatatcct tcatatccgt
     4621 aatcattgcc gacatacccg aaacaaagca ttgtttaccg gcaaccgaac ggatttcttc
     4681 gatagcgttc attgtttcat cagctgatgt tgaggtatcg aagaataccg ccatcattgt
     4741 tgcatttccc gaattgaacg catcataaat tttgtcgggc aacatttcct ttggaattga
     4801 gatgtcggca aaatcgtcat accaaagaac cgtatcaaca tggtcgattt ctttgatttt
     4861 ttcctctgtt gccgcaacat ccttatcatc catattctca aagatgagga atgaaaaagc
     4921 accctttcca aagtcatcaa gcaaataatc ctgacccttg attgtgtcga ggttatcggg
     4981 aagataacta agaatgtcgt aattaaccct tgtaaatgcc attcccaagg ctgacggaat
     5041 catcagcgca acggcaagta taaggattac gaaacggctt ttaacaaccg ctttccctaa
     5101 cttcatattt catactcctt catatatgat ttgcagactg cactctgcat tcaaaggaac
     5161 gatccgagag gtgaaccgta tttcctttgt ccataacata ttctattacg gctttgttaa
     5221 aatttaaaca gacacaaaaa atgccgtgtc aggaatttag acacggcaat aaatttgtac
     5281 ggaaatcaga cattttgtta aattcgtctg catttcagac acgcaagcaa gcgcaaatat
     5341 tcaattttaa ccaataaaac agcaatcagg cattgatttt ggcacatctg tccaatgcag
     5401 actccattga accctcaaac agctcacaaa tgcgtttcat cttaattctg aggtcatatt
     5461 tcatacctcc gccgagccag tcgataataa aaccgacaag ctgacacttg taatacataa
     5521 tcattgcgtc aaggtcatct tcggcaatat tttttgtaat ttcaagcttg tcaaaatagc
     5581 tttttatagc gtgagtacaa atcctgttga gatacacatc aaatgtagtt ctgttcgcag
     5641 aattgtaaat gtgctgaatc accgacttgt tagaaaaagc atagtcaatt acagaaatca
     5701 ggctttcata aacagaagta ttgttgtcgg tttcggtcgc aacaaatctg tcagcctctt
     5761 caacgagaat ttcctcaaga agcgacggaa tgtcgtcaaa atggtagtag aaagaattac
     5821 ggttaattcc gcattcttca acaatttcct tgactgtaat tttattcaac ggctttttat
     5881 tcaagagcga aataaatgtc gccttgatag ccgccttggt aaatgtcggc atagtgtccc
     5941 ccctgaaaat tgcaaaacat ccgtgtgcag acggatattt ccgttccgaa ttttttcata
     6001 cacaatatat gcagccgatt gctacactaa tcaaagtaaa ttgtatcaca attattcaaa
     6061 aatagcaata acaagcatat taacaaaatc aggggtttgt tttgtatttc ctatgtaaaa
     6121 aagccccgaa attgatgtta cccccaaaaa agtcagacca aaaatctaat gattttggag
     6181 gttgattcaa aatcgtggct ttatatttat aaaattataa attcaatatc tttcgggaaa
     6241 tcattttgca cactgcaaag gctgatttca tagtccgaaa atttatattg tttcatataa
     6301 tattcggttt gattttcaga gaattttccg ctgctgacat caacactttt tgcgccacgg
     6361 tgaaatccgt cacccataca ttttaggagt gcctcttttt tagtccacaa ttcaaaaaat
     6421 gtgccgagcc tgtcaaaagc gttgccgagc aatttcatct cgttgtcgca gaaaacaatt
     6481 ttgccgagcc ttatgccctt gagaaatcga atttcttcaa tatcacaacc gacttcggaa
     6541 tccgacaacg caaaaagcac atagcttccg ctgtgcgaaa tattgaaaca aagtccgttg
     6601 tcacattcgg gcttgccgaa ttcattcacg gtgatttttg caccgccaag aaatttgttc
     6661 ataaacaagc cgcctgcaat gcaacgcagt ttatcgtcag ctctgcggca tctttgaaca
     6721 cgctccgccc tttcgggaga aagctcaccg actctgtcaa aagaaatttt gcggatatcg
     6781 gttataaaaa gccgtgtttt catatcaata gccacgaagt gcgtcaagcg aatcatcgga
     6841 cataatccaa tcgttcacat caatttcagt aaattcggtt ttgttgtttc tgacaataac
     6901 cttgtcaata cctgcgttga taaccatacg cttgcacatt gcacacgaag atgtgttctg
     6961 aacatactcg ccgtttgaca tctccttgcc gacaagataa agcgttgcgc caatcatctg
     7021 ttctcttgat gcatgaataa tagcgttcgc ctcggcgtga acgcttctgc aaagttcata
     7081 acgctcgcca cggggaatgt tgcgttcctg acggatacaa aatcccaaat cacagcaatt
     7141 ttttctgcct ctcggagcgc ctgtatagcc tgttgaaaca atctggtcgt tgttcacgat
     7201 aattgcacca aaatttctgc gcaggcaagt tcccctttca agcacagttt cggcaatgtc
     7261 aagataataa ttaattttgt caattctgtc catataaaaa caccgtccgt tatcataatt
     7321 tcaaattcgg tcaagtatat tttagagtag tcgaaagcag tatcagcctt tgagcttttc
     7381 aacacaatct ctgcatacat attttccgcc gaatgcaatc agattatcaa gagtattgca
     7441 aaaaacgcac ttgctctctg cctttttaat cgtaacgcac tcatcgtcag cctcaatgtg
     7501 aagaatgtca cccggcttaa tgttgaggtg gttgcggata tcctttgata ttacaattct
     7561 gcctgcttta tcaatttccc taaagtttgt ataaatcatt ttaattcacc ataatcccta
     7621 ttttttaatt caccataatc cctattttta cctatactac catttttcgg gatttttgtc
     7681 aacgatttta ttaaataatt tgattacaaa aatgacaata ttgtacattt tgtcgttaat
     7741 tgcagcaaaa ggagtaaaaa tgctgaatta tatctggagc ggactcattg ccgtgagcgt
     7801 attgtgtgcg ttgtttatgg gcaacaccgc cgacatttca aacgcactca tcgaaagcgg
     7861 agcgtcatca atacagctta ttctcacaat ggcaggcata atctgccttt ggtcgggaat
     7921 tatgaaaatc gcagtcgaaa gcggtttgac aaacattttt gcaaaaatct ttgcaccgtt
     7981 gctccgtccg ctttttccga ggcttgataa aaaaagtgac gcatttcaaa gcatcactat
     8041 gaatatcagt gcaaatctgc tcggactcgg caatgccgca acacctttcg gactaaaagc
     8101 aatggaacag ctgcacacgc tcaatgaccg ccgtgacatt gcgagcaacg aaatggttgt
     8161 ttttgtagtt atgaacacag cgtcactcca gctgttaccc acaacgcttg ccgcactccg
     8221 ccaatcctac ggaagctctg cgccttttga aattatcgtt cccgtctgga taagctctgc
     8281 gtgtgcgttg acggtggcat tgctgattgc ctgtacgctg aatatcaggc aaggtaaaat
     8341 ttgagaaatg tggtgagcaa atggaatttg taatgccggc atttgcgtgt attgttgtgg
     8401 tattcgggct gataaaaaaa gttcccgtat tcgatgtgtt catctcggga gcaaaagagg
     8461 gagtaaaact tctgtacaac attgccccta caattatggg acttgtgttt gccgttgacc
     8521 ttttgcagtc aagtggagca attgacgcaa tatgcgcact gattcagccg acagctgaat
     8581 ttttaggatt tccgaaagaa attgttccgc tcgtactcct ccgccccgtt tcgggaagcg
     8641 gttcgaccgc actcctcaca gccctttaca aaacctgcgg tcccgacagc tttgcgggca
     8701 gaacagcatc cgtactcgca ggttcaagcg aaacaacctt ctacgcaatc gcaatgtact
     8761 ttggctgtat ccgtgtcaaa aaaattcgcc acaccctctt tgccgccata accgccgacc
     8821 taaccgccgc aattatgagt gttataacgg tcagattgtg gtttaatatg tgattgtaaa
     8881 gtgttcccat ttcggcacac tttccctttt ctttgggttt ttgctcccat ttcggcacga
     8941 ttttatttta acatttttca atttatcatt ttccttgcca aacagagtta ttattccaaa
     9001 aatccgatac taaataaaaa agccctttga gaaaagattt ctcaaagggc ttgataaact
     9061 ggaatttata ttgcaatcat tttttcttta attctagaat attcttctcg atctgctgta
     9121 taacccccct gaaatcatca tccgtttttc ctgtcggatc ttcaagtttc cagttgtcat
     9181 caaacgctct gccaataaac ggacaaccta catcacaacc catggagatg gagatgtctg
     9241 gctccgggat gcttgatatc agcttagaat actgcttttt ctccatgtcg atgccgtaaa
     9301 gctccttcat gagacggacg gcatcctgat tgatctgggg cgcagcaagt atgatggaat
     9361 ccttcaggaa atcaatgccc gtatgattct gtataa
//
LOCUS       DAOMVE010000063         9335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1859_length_9335_cov_3.117888, whole genome shotgun sequence.
ACCESSION   DAOMVE010000063 DAOMVE010000000
VERSION     DAOMVE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9335
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1859_length_9335_cov_3.117888"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1232..1804
                     /locus_tag="PLS64_04940"
     CDS             1232..1804
                     /locus_tag="PLS64_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HRL43209.1"
                     /translation="MNDKLHIRTAQISDAEELLAIYKPYIENTAITFEYDVPSIEEFA
                     NRIKTTLKRYPYIVAELNGKIVGYAYAGVFKGRKAYDWSVETSIYIRLDNHGNGIGRK
                     LYEKLEEILAKQGVTNVNACIAYTEHEDKHLHNDSVCFHKKLGYKTVGIFHKCAYKFD
                     KWYDMIWMEKFIAGHSANPNDIIPYPVLNC"
     assembly_gap    1806..1815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1941..3824)
                     /locus_tag="PLS64_04945"
     CDS             complement(1941..3824)
                     /locus_tag="PLS64_04945"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HRL43210.1"
                     /translation="MSELLLNQFEQDRALVALRYKNLNIRKLFGKSVFIAGGGELAFS
                     LVSSLRTVNLKKQADIAVFLLVEDNESYDRRFDYIDSSDFSIVKYSSLNAVNKCGDIL
                     IETGFLLSDRVEDVDVFKNHINRANNIISAVNALKIKETVLVSDASIYGTLGKDFVIS
                     EKEKTHSAFNSDSLKAMLIQSVENLYFSASHMYDFSIKAVRSGKIISANSSSDFVRNM
                     LESAVHGKSLNVKNKSPKVSYISINDLISAVLFVLCNGENNQVYNACSDTSTVNSAEF
                     SLTLSDAFDECEVNITSAGDSTDGCAIDCTRLKKLGWLSMVNYKDALLISGHEVMDDD
                     SIFMFSDSYDGKLNDIQQILLGFLLEVDRICKKHNIKYFLGGGSLLGAVRHKGFIPWD
                     DDADVMMLRKDYDRFLSVLPSELPNYLFAQTQKNEKDSHFPFTKLRINDTLLSTEFTS
                     RFPNIHNGIFLDVLAQDYTSNNAFLRKIHMKATASSRWLVLDKWRGTSVNANSRFSSL
                     CANILRKIFPLGFLQKVQNKLISLHKNMKNPKYLFDSMGRNVSRGAFPAEWLDEAIWV
                     DFENAKLPIPKEYDKYLKYLYGDYMEMIPVSERHVSHDIKQIDLGEYAGYVCKDSFAK
                     LEK"
     gene            complement(3848..4927)
                     /locus_tag="PLS64_04950"
     CDS             complement(3848..4927)
                     /locus_tag="PLS64_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRL43211.1"
                     /translation="MDYNVQSNIKKTVFSDIKEITNTNVDWNRFNGKTVLITGAGGFI
                     GYYLVLTFLARNDFYNNNIKLLAFVRNRERAEKKFGVLLERDDIELVVADVTNKIECE
                     RADFIIHAASQASNIQFETDPVGTINANLSGTSNILDFALESKSESVLVVSSLKVYGA
                     LHNGKNSISEDDIGYIDFTSYKNCYAVGKRASETLAASYCKQFGLNVKIARPSYIYGA
                     SSLTDDRVWAQFIANVVRHENILLKSNGAANRSFCYVTDTVSALIYIMLNGENVTPYN
                     ISYEKSDTTIRNFAKTACEVFPERNMTLAFANPEDEKEPEVDTSALAKTPEILDSSRL
                     CSLGWKPLVDLKEGIRRAVGILEEN"
     gene            complement(4917..5657)
                     /locus_tag="PLS64_04955"
     CDS             complement(4917..5657)
                     /locus_tag="PLS64_04955"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HRL43212.1"
                     /translation="MVTAMLFAGGIGARMKYSDMPKQFLEVGGKPIIIHTMEHFARHP
                     KVDAIVVACKADWIGYLNELIEAFNVPKVKAVVPGGANGFDSIHNGVMATAEFSKDPE
                     DIILICDGVRPMLSEELITNCIKDTKKYKTAVPVTPSIDSLLYSEDGETCGKSYQRST
                     MYITQAPQGYTMERILWAHEEAYKRGITNPVSSSELFIELGEKVHLFLGERFNIKVTT
                     PEDLTTLRSQFYYENYKQFAKEELKYGL"
     gene            6070..7137
                     /gene="pyrB"
                     /locus_tag="PLS64_04960"
     CDS             6070..7137
                     /gene="pyrB"
                     /locus_tag="PLS64_04960"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HRL43213.1"
                     /translation="MFDKNLIDLSVMSTEQIDKIIRKAKKIMQSPADYRHACDGKILA
                     TLFYEPSTRTQMSFQTAMLKLGGRTIGFDNPMNSSVSKGESLKDTITIVGEYADIIAM
                     RHPVEGTAAAASLYAGVPVINCGDGGHLHPTQTLADIITLSCEKGRLDNLRVGVCGDL
                     LNGRTVHSLIKTLAKYPNNSFVLISTKELSVPLYVIDILEKNGCKYEISHSLADAITN
                     LDVLYMTRIQRERFASEEDYQAQKNVFVLDKEKLDKAKEDMIILHPLPRVNEITVDID
                     DDPRALYFKQAQYGMYGRMALILLLLQDDEFFVENRPFVVSNHHCNNPKCITQQEPYL
                     PNLSYEKLGMVMCGYCDKRID"
     gene            7148..7738
                     /locus_tag="PLS64_04965"
     CDS             7148..7738
                     /locus_tag="PLS64_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRL43214.1"
                     /translation="MSEHKKKKNPAATMMMTSLAYIVLGIVTVVFPQKVLDVLCYALG
                     GAMTVYGLFNIISFFVDREGGMYFELVIGVISTGFGIFALFSPTSIAAIINIPIGIII
                     IVDSIMDMKHSLTLRSLGMRKWWIPFIIAIVMIMFSLTTIFFTNIFGALLMIALGIAL
                     IYEGVSGFSLIILLSRYHKEYDRNQRMIDAEATDIN"
     gene            complement(7781..>9335)
                     /locus_tag="PLS64_04970"
     CDS             complement(7781..>9335)
                     /locus_tag="PLS64_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRL43215.1"
                     /translation="FVDELKILRDCACTNSVDALISKACEMTGFMSISLAVSGNDSAL
                     KNLELLREYARSFESNGYKTLSDFISYTDKLIANKTNLDASSQNEGDSANAVRVLSIH
                     ASKGLEFPVCFIADITHQFNKTDLRNDILLDSKAGLGIKTQSNGVVYNTFPRLATGLK
                     IEENLIAEEMRVLYVALTRAKEKLICVGAVKKCSDYLERLYSKLVSESVIEPYTVTSC
                     QSYCDWLCLCALVHPSLNNLRNDIMPGSKVIPHNGESDWKLQIIVDEKMIDKDNVFED
                     DITSTNLLQVADVSDDTFDYAKLLKKNLDFKYRNRDILSLPQKVSASDIAHSQNGDYF
                     EKILSKPLFIAEQNSAPVARGTAHHKFLQYCNFEQARASLDTEIDRLLNDGKLTQEQV
                     DMIDRKTLNEFLSTKLIDRIIASQLVMREKRFTARLKPSEVFDEYKGVKTDATVIIQG
                     AVDLAFVEDGKLVIVDYKTDRVKDITKLAGLYKKQLDLYKSAMEQSEELTVKECILCS
                     IHLGAYITV"
BASE COUNT         2856 a         1819 c         1650 g         3000 t
ORIGIN      
        1 aagcgagatt taatttaagt tcttatctca tcggaattat ctcctgacac tttagcttcg
       61 actaactgtg tttatacatc tgagtatgta cactacaatc aaactataag acctaaattc
      121 tcattggaat aatctcctgt tgtttgcaga tttctatgtc atcccgacat tttctttact
      181 gctaatcaat gctttattta cgacttatat taacttttcg tcaaatcaat atcagtaacc
      241 gtataccgtc aattcccgtt aatggatttt atttttgacc ttgtatcggt ttaaaatctc
      301 aagcatacat cggaatatct ccaatcttgt cgcataagcg agatttaatt taagttctta
      361 tctcatcgga attatctcct gacactttag tttaccttga attttagaaa tcagattgaa
      421 atttacggtt tcactctgta ttataagttt ccaaatttac attccgtttg aactgcttat
      481 taaattcagc ctcttacatt ctttaaattc agactactct gtgtgccgtt gtataagctg
      541 ttcatccatt ggttttagta ttaatttcca ttggacttat ctccgtttcg gacttatttt
      601 gcttaaataa cgattaagct ttcattggac taaccaccaa tttttagatt tcaatatata
      661 ttaagttata attaaaattc cgttctcccg agtattaaat tcgataacct ttaatgctta
      721 attggacgaa tttcaatcat aagatgtaga ttgctaaaga cgaattcgga atgctcttgc
      781 gattaaaaat taataaatat gtttttataa catcaaaata atcagctgtt ccgacaaaac
      841 aagtaagaat tagaagatca tctcttagaa tacacgggct gtagagttag gtggcaagct
      901 ccgcatataa tctctgattt gtttgaagta aggttttctt gctttcattt ttaaaacctc
      961 cttaagttct tttctttgtc tatattatac agttagttgt ccacattgtc aacactttta
     1021 ttaaaaatat ttataatttt atttttgaaa aatctattga agtaaaattt acattgtgtt
     1081 ataataaaaa tgttggggtg tttatcagtc ggcaactttt ttgagtgccg acttttttct
     1141 tttttatata cttcaaaatg cctaaatatt gattataact aaactttagt gtattattat
     1201 gatgtcataa agtattaaag aggtcataat tgtgaacgat aaattgcata tcagaacggc
     1261 acaaataagc gacgccgaag aattgctcgc tatatacaaa ccatatattg aaaacacggc
     1321 aatcacattt gaatacgatg ttccaagcat tgaagaattt gcaaatcgca taaaaactac
     1381 gctcaaaagg tacccttata ttgtagccga actcaacgga aaaattgtcg gctatgcgta
     1441 tgccggagtt ttcaaaggca gaaaagctta tgattggtca gttgaaacaa gtatttatat
     1501 ccgccttgat aatcacggaa acggaatcgg cagaaaactc tacgaaaagc tcgaagaaat
     1561 tctagcaaag cagggtgtga caaatgtaaa tgcctgtatt gcgtacactg aacatgagga
     1621 taaacatctg cataacgaca gtgtttgctt tcacaaaaaa ctcggttata aaactgtcgg
     1681 tatttttcat aaatgtgcct acaaatttga taagtggtac gatatgatat ggatggaaaa
     1741 atttattgcc ggacacagtg ctaatccgaa tgatattatc ccctaccctg tgctgaattg
     1801 ttaacnnnnn nnnnnaagtt cgataaaaaa cttttcgggg attgaatttg tcaataaaaa
     1861 tgggtactgt cttaatattg cttacaatta ctataagctg aaatttttaa taatatccca
     1921 cttaataatg caatttagaa ttatttttca agctttgcaa aactgtcttt gcagacatat
     1981 cctgcatatt ctccgagatc aatttgcttg atatcgtgac ttacatgtct ttcgcttaca
     2041 ggaatcattt ccatatagtc cccgtaaaga tacttcaggt acttatcata ttctttagga
     2101 ataggcagtt ttgcattttc aaaatcaacc catatcgcct catcaagcca ttcggcaggg
     2161 aaagctcctc ttgaaacatt tcttcccata ctgtcaaaca gatatttcgg atttttcata
     2221 ttcttatgaa gtgaaataag tttgttttga accttttgca aaaatccaag cggaaaaatc
     2281 tttctcagaa tattcgcaca caaagaagaa aatctgctgt ttgcattcac cgaagtgcct
     2341 ctccacttat caagcaccag ccatcttgag cttgcagttg ctttcatatg aatttttcgt
     2401 aagaaagcat tgtttgaagt ataatcctgt gcaagcacat caagaaaaat tccgttatga
     2461 atattgggaa atcttgatgt aaactcagta gaaagcagag tatcgtttat tcgcagtttt
     2521 gtaaaaggaa aatggctgtc tttttcattt ttttgagtct gtgcaaaaag ataattcggg
     2581 agttcactcg gcaataccga aagaaacctg tcgtaatctt ttctaagcat cataacatct
     2641 gcatcatcgt cccacggaat aaagccttta tgccttactg cacccaaaag acttcctccg
     2701 cccaggaaat actttatgtt atgctttttg caaattctgt caacctcaag taaaaagccc
     2761 agaagtatct gttgaatatc attaagcttg ccgtcatatg aatcggaaaa cataaatata
     2821 ctgtcatcat ccattacctc gtgtcccgaa ataaggagtg cgtccttgta gttgaccatt
     2881 gataaccaac caagcttttt cagtcttgtg cagtcaatgg cacagccgtc cgtagaatca
     2941 cctgcagaag ttatgttaac ctcacactca tcaaaagcat ccgaaagcgt aagtgaaaat
     3001 tctgccgaat ttacggttga agtatctgag caggcgttat acacctgatt gttctcaccg
     3061 ttacaaagaa caaacagaac agcagaaatc aagtcattaa tacttatata agacactttg
     3121 ggtgatttgt tcttaacatt caaagatttt ccgtgaacag ccgattccag catattcctt
     3181 acaaaatcgg aacttgaatt tgcagatata atcttgccgc ttctaactgc tttgattgaa
     3241 aaatcataca tatgagatgc agaaaaataa agattctcaa cagactgaat cagcattgct
     3301 tttaatgaat ccgaattaaa agcggaatgt gttttttctt tttcggaaat aacaaaatct
     3361 tttccgagtg ttccgtaaat tgaggcgtct gacacaagaa cagtttcttt tatctttaac
     3421 gcattgacag ccgagataat attatttgcc ctgttgatat gatttttaaa tacatcaaca
     3481 tcttcaactc tatcagaaag caaaaatccc gtttcaatga gtatatctcc gcatttatta
     3541 accgcattaa gtgaagaata ctttacgatg gaaaaatctg atgaatcgat ataatcgaat
     3601 ctcctgtcat aactttcgtt atcctctaca agcaagaata cagctatgtc tgcttgtttt
     3661 tttaagttaa ccgtgcgaag cgatgataca agagaaaagg cgagttctcc gcctcctgct
     3721 ataaaaacag attttccgaa taatttacgg atgttcaaat tcttataacg caaagctaca
     3781 agtgctctgt cctgttcaaa ttggttaagt aacaactcag acataactgt attcctaatt
     3841 tctgttatta attttcttca agaattccga ctgctcttct tattccttct ttaaggtcaa
     3901 caagaggctt ccatccaaga ctgcagagtc ttgaactgtc aagaatttcg ggagtttttg
     3961 caagagcgga agtatcaacc tcaggctcct tttcatcttc gggattggca aaagccaaag
     4021 tcatatttct ctcagggaaa acctcacaag ctgtctttgc aaaatttctt attgttgtat
     4081 ctgatttttc ataagaaatg ttatacggtg taacattttc accgttaagc ataatgtata
     4141 tgagagcaga aaccgtgtct gtaacatagc agaatgaacg gttagccgca ccgttgcttt
     4201 tgagaagaat attttcgtga cgaacaacat ttgcaataaa ctgcgcccag acacgatcat
     4261 ccgtaagact gctggcgccg taaatatagc tcggtcttgc aatctttaca ttgagtccaa
     4321 actgtttgca atagcttgcg gcaagagttt cagacgctct cttacccact gcataacaat
     4381 ttttatagga agtaaaatca atataaccga tatcatcttc ggaaatactg tttttaccgt
     4441 tgtgcaaagc accgtacact ttaaggcttg aaacaacaag tacagactca cttttgcttt
     4501 caagagcaaa atcaagaatg tttgatgtac cgctgagatt tgcgttaata gtgcctacag
     4561 gatcggtttc aaattgaatg ttacttgcct gacttgcggc atgaataata aaatccgctc
     4621 tctcacattc aatcttattt gtaacatcgg caacaacaag ctcaatgtca tctctttcaa
     4681 gaagaacgcc aaactttttc tcggctctct ctctgtttct tacgaacgca aggagcttga
     4741 tgttattgtt ataaaagtca tttcttgcga gaaatgtgag aacaagataa taaccgataa
     4801 atcctcctgc accggttata agaacagttt taccgttaaa tctgttccaa tcaacatttg
     4861 tatttgtaat ttctttaata tcggaaaaaa ccgtcttttt tatattactt tgaacattat
     4921 aatccatatt taagttcttc ctttgcaaac tgcttgtagt tttcataata gaactgtgat
     4981 cgcaatgttg tgagatcctc aggtgttgtt accttaatgt tgaatctctc gccaaggaaa
     5041 agatgaacct tttcgccgag ctcaataaaa agttcacttg aggaaacagg gtttgtaata
     5101 cctctcttat aagcttcttc gtgagcccaa agaattcttt ccattgtata tccctgcgga
     5161 gcctgtgtaa tgtacattgt acttctctgg taactcttgc cgcaggtttc tccgtcctca
     5221 ctgtaaagaa gtgaatcaat actcggagta acaggaacgg ctgttttgta cttttttgta
     5281 tccttaatac agttagtgat gagttcttcg ctgagcatag gacgaacacc gtcacagatg
     5341 aggatgatat cctcaggatc ttttgaaaat tcggcagtag ccattacgcc gttgtgaata
     5401 gaatcaaaac cgttagcacc gcccggaaca acagccttta cctttggaac attgaatgcc
     5461 tcaataagct cattaaggta gccgatccag tcagccttac aggcaacaac aatcgcatca
     5521 accttaggat gacgggcaaa atgctccatt gtgtgaataa taatcggctt tccgccaacc
     5581 tcaagaaact gtttaggcat atcggaatac ttcattcttg caccgatacc gcctgcaaat
     5641 aacatagctg taaccatagt aagacaatcc tttcaaaaca caaatcagac ttcactttac
     5701 aaaatgcagt caaatttatt tttttaacag cagaattacg cagtaaaaaa cgcgtctgca
     5761 ctttgatata actattaata tgataccaca ttaatgcatt ttttgtcaac aaaaatactt
     5821 tgtacgaatg ttttaatcga ctttgccggt ttacggtgaa tttcttatac cgtattttca
     5881 aaatgctttt tatttaggca catcattttg acgcacaatt taatattttt cataccttat
     5941 aaaattaatg cgagaaaatg cgggaatgag taaaattgtt ttattcttta ttcccatttt
     6001 tcttttaagt atatataaat acagaaaatt ttgataaaat agaaaaaatt gcagaaagct
     6061 gtgttattta tgtttgacaa aaatttgatt gatttatcgg ttatgtctac cgagcagatt
     6121 gacaaaatta tcagaaaagc taaaaagatt atgcagagtc ccgccgacta tcgacacgcc
     6181 tgtgacggca agattcttgc tactttattt tatgaaccga gtacaagaac acagatgtca
     6241 ttccagacgg ctatgcttaa actcggcgga agaacaatcg gttttgacaa tccgatgaat
     6301 tcatccgttt caaagggcga aagccttaaa gatacaatta caattgtcgg cgaatatgcc
     6361 gatattatcg caatgcgtca tcctgttgag ggtactgccg ctgcggcatc tctctatgca
     6421 ggtgttcccg ttatcaactg cggtgacggc ggtcaccttc atccaactca gacgcttgcc
     6481 gacattatca ctctcagctg tgaaaaaggc agacttgata acctcagagt aggtgtatgc
     6541 ggtgaccttc tcaacggcag aacagttcat tcgcttatca agacacttgc aaaatatccg
     6601 aacaacagct ttgtacttat ctcaacaaag gaactcagcg ttccgcttta tgttattgac
     6661 attcttgaaa agaacggctg taagtatgaa atttctcata gccttgcaga tgcaatcaca
     6721 aaccttgatg ttctgtacat gacaagaatt cagcgtgaga gatttgcaag cgaagaagat
     6781 tatcaggcac agaaaaatgt ttttgttctc gacaaagaaa aacttgacaa ggcaaaagaa
     6841 gatatgataa ttcttcaccc attgccgaga gtaaacgaaa ttacagttga tattgatgat
     6901 gacccgagag cgttgtattt caaacaggct cagtacggta tgtacggcag aatggcactt
     6961 attcttctgc ttttgcagga tgatgagttc tttgttgaaa acagaccgtt tgttgtatcg
     7021 aatcatcact gcaacaatcc gaaatgtatc acacagcagg agccatatct tccgaacctc
     7081 agctacgaaa agcttggtat ggtaatgtgc ggttactgcg acaagagaat agactgaggt
     7141 gtattaaatg agtgaacata agaaaaagaa aaatccggcg gctactatga tgatgacctc
     7201 tctcgcctat atcgtactcg gcattgtgac ggttgtcttt ccgcaaaagg tgcttgatgt
     7261 tttgtgctat gcactcggcg gtgcaatgac tgtttacgga ctgttcaaca taatttcttt
     7321 ctttgttgac agagaaggcg gaatgtactt tgaacttgta ataggcgtta tttcaaccgg
     7381 attcggtata tttgctttgt tttcacccac ttcaattgcg gcaataatca atattccgat
     7441 tggcattatc attattgttg acagcattat ggatatgaaa cattcgctca cgctgagatc
     7501 gctcggtatg agaaaatggt ggattccgtt tattatagca attgtaatga ttatgttcag
     7561 tcttacaaca attttcttta caaacatttt tggtgcattg cttatgattg cactcggaat
     7621 tgcgcttatt tatgaaggtg tttcgggatt ttcacttatt attctgctca gccgttatca
     7681 caaagaatac gaccgcaatc aaagaatgat tgatgcagaa gcaaccgata taaactgaat
     7741 atgattaaac cgtccgcaga atttacggac ggttttcttt ttatacggta atgtatgcgc
     7801 cgaggtggat actgcaaaga atacattctt taacggtaag ttcttcggac tgctccatcg
     7861 ccgatttgta caaatcaagt tgcttcttgt aaagtcctgc aagttttgtt atatctttta
     7921 ccctgtcggt tttgtaatcg acaataacaa gctttccgtc ctcaacaaac gcaagatcaa
     7981 ccgcaccctg aattattact gttgcatcgg tttttacacc tttatattca tcgaatactt
     8041 ccgacggttt aagccttgcg gtaaatcgtt tttcacgcat tacaagctgt gatgcaatta
     8101 tacggtcaat tagtttggtt gacagaaatt cattaagtgt ttttctgtca atcatatcaa
     8161 cctgttcctg tgtaagtttt ccgtcattta aaagacggtc tatttcagta tcaagacttg
     8221 ctcttgcctg ttcaaaattg cagtattgca aaaacttgtg gtgagcagta cctcttgcaa
     8281 caggagcgga attttgttca gctatgaata acggctttga aagaatcttt tcaaaataat
     8341 caccgttctg actgtgtgca atatcggatg cactgacctt ttgcgggagt gaaagaatat
     8401 ccctgtttct gtatttgaaa tcaagattct ttttcaaaag ttttgcataa tcaaaagtat
     8461 catcggatac atcggcaacc tgtaacagat ttgttgaagt aatatcgtcc tcaaagacat
     8521 tatccttatc aatcattttt tcatcaacga taatctgcaa tttccaatct gattctccgt
     8581 tgtgcggaat tacctttgaa ccaggcataa tatcgttacg cagattattg agtgacgggt
     8641 gaactaatgc acaaagacag agccagtcgc aatagctttg acagcttgtg acagtatagg
     8701 gttcaattac actttcggac acaagctttg aatacagcct ttcaagataa tcactgcact
     8761 tctttactgc accgacacag ataagctttt cctttgctct tgtgagtgca acataaagca
     8821 cacgcatttc ctcggcaata aggttttctt caattttaag ccctgttgca aggcgtggga
     8881 atgtgttgta aacaacacca ttgctctgag tcttgatgcc gagtcctgcc ttgctgtcga
     8941 gaagaatatc gtttctaagg tcggttttat tgaattggtg ggttatgtcg gcaataaagc
     9001 aaaccggaaa ttcaagtccc tttgacgcat ggatactgag aactctcacg gcatttgcag
     9061 agtcgccctc attttgtgat gatgcgtcaa ggtttgtctt attggcaata agcttgtcgg
     9121 tatatgaaat aaaatcagaa agggttttat agccgttgct ctcaaaactt cttgcatatt
     9181 cacgcaacag ctccaggttt ttaagtgccg aatcattacc gctgactgca agcgaaatag
     9241 acataaatcc tgtcatttca cacgcctttg aaatgagagc atcaacggaa tttgtacagg
     9301 cacaatcacg cagaatttta agttcatcaa caaat
//
LOCUS       DAOMVE010000064         9319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1867_length_9319_cov_2.825561, whole genome shotgun sequence.
ACCESSION   DAOMVE010000064 DAOMVE010000000
VERSION     DAOMVE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1867_length_9319_cov_2.825561"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLS64_04975"
     CDS             complement(<1..796)
                     /locus_tag="PLS64_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43216.1"
                     /translation="MKTKISHLGRSTVSVILAVMMLLSTMLIGTVSTVNATVSGLSEI
                     YFTPSIKWQEGGANFRLNVQDSSNNWHKITMTKVDENKPIYKGELDSNTTYNVVQFLR
                     YNNDYTKEWDYSKSCSDIPSGMNYYTMTTETYKDWAIDNSNGTWSYYFDSDPVADSAS
                     LTASSETVKVGDSVTLTAVADNPISTNLKYTFTKTSGGDATVVQDNNKLTVTPTVAGT
                     YKYTVTVSADGYSPVTSTEVSFTVTDSVKYYLAGRVKQDGSDTWVDS"
     assembly_gap    1286..1298
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1427..2230)
                     /locus_tag="PLS64_04980"
     CDS             complement(1427..2230)
                     /locus_tag="PLS64_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008716470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43217.1"
                     /translation="MLEIKNVWKTFNKGTVNEKQALCGVNLTLNDGDFCTVIGGNGAG
                     KSTTLNAIAGVWPVDCGQILIDGKDISALPEHKRAPYLGRVFQDPMTGTVADMEIIEN
                     LAIAARRGKKRGLSWGVKADERIRYRQMLAEFDLGLEDRMSSKVGLLSGGQRQAITLL
                     MATIKKPKLLLLDEHTAALDPKTAAKVLELSDKIIAENNLTALMVTHNMHDAITHGNR
                     LIMMSDGKIILDISGEEKKNLTVEHLLEMFEKASGSKLENDRMLLSKDK"
     gene            complement(2233..3198)
                     /locus_tag="PLS64_04985"
     CDS             complement(2233..3198)
                     /locus_tag="PLS64_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL43218.1"
                     /translation="MDFLANYFGSLNPMSFLSALPGNIAQGIIWGLMGLGVFLTFKLL
                     NFADLSVDGSFATGGAVTAVMLINGCPVWATIIVAILAGLAAGLVTGLLHTLLGIPDI
                     LAGILTQIALYSINLNIMGKSNLPISYRNYTLVVSASNINMAILVGLGICAVVIAAMY
                     WYFGTEQGSTIRSTGSNPAMSKAQGININFTKVIALALSNGLVALSGSLFAQYQGFAD
                     INMGRGAIVIGLAAVIIGEVLGEAIFRKHMNFIIRMVFVIVGGILYYIAMGIVLWLKM
                     PTDDTKLFTAIIVAIFLAVPYLQKKRKTSFKKVAKMNAKNAQKEG"
     gene            complement(3275..4279)
                     /locus_tag="PLS64_04990"
     CDS             complement(3275..4279)
                     /locus_tag="PLS64_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRL43219.1"
                     /translation="MKKAKKIVAVALAAASMSLAFAGCGGSAESSKADNSATDGKVYN
                     IGICQLVQHEALDAATKGFKDYLTEKLGADNVKFDEQNAQGDSATCATICNQFVSSNY
                     DLILGNGTAALQAAYTATPNIPILGTSITDYGTALDKSDWNGVSGMNVSGTTDLAPLD
                     QQAAMLKELFPDAKNVGILYCSAEANSKYQCDTITPYFKDAGYTVKSYSFADSNDVAA
                     VAKTACDESDVLYIPTDNTAASNTGIIDNVATAAKTPIVAGEEGICKGCGVATLSISY
                     DELGRKTGEMAYNILVKGEDITKMKVEAAPNVTKEYIKDRCDTYGIKVPDGYTEIKAE
                     "
     gene            complement(4452..7976)
                     /locus_tag="PLS64_04995"
     CDS             complement(4452..7976)
                     /locus_tag="PLS64_04995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRL43220.1"
                     /translation="MAFTHLHLHTEYSLLDGACRLSRLAKAAKEKGMTALAITDHGVM
                     YGAVDFYKECKKQGIKPIIGCEVYVAPQSRFDKTSSYGKYYHMVLLCKNHTGYQNLIK
                     LVSKGFTEGFYTKPRIDDELLAEYHEGLVCLSACLAGEIPQKLLDGDYAGAKSKAEFY
                     RDIFGSENFFIELQDHGIEEQKRIIPSLIKIAREIGVDIVATNDCHYIEKQDSTVHNI
                     LLCIQTNRTVNDSDRMEFKTSEFYLKTEDEMREVFASYPEAIDNTQKIADMCNVDFEF
                     GVRKLPRFDVPNNEDHLHYFRRKCYEGLYKHYGENPDKALIDRLEYEINTVSQMGFVD
                     YYLIVNDFVSYAKSCQIPVGPGRGSGAGSLCAYCIGITAIDPIKYNLLFERFLNPERV
                     SMPDFDVDFCKERRGEVIDYVVRKYGEDHVAQIISFGTMAARGAIRDVGRVLEIPYAT
                     VDSVAKLVPMEHDMTIEEALKLSPDLRSRCNNDPQIKKLLDVAMSIEGMPRHATMHAA
                     GVVITDKPVSDYVPLSKNDDNVVTQFTMTALEELGLLKMDFLGLRNLTVINDAEKQIK
                     HKNPDYNPDMVDENDKKVFEMFSKGYTEGVFQFESQGMKNVLTQLKPERIEDLIAVTA
                     LYRPGPMDSIPVYIDCRHNPSHIRYKHPLLKEILGVTSGCIIYQEQVMQIFRTLAGYS
                     LGRADIVRRAMSKKKLAVMESEREIFIHGLTDDDGNIIVEGCIRRGVDEKTAISVYDE
                     MESFAHYAFNKSHAAAYANISYKTAWLKCHYPREYMAALLSSVLDNQNKLASYITECK
                     RLGIRVLPPNVNESNLHFTVSGNDIRYGLLAVKNLGRNFIDEIISERINNPYEDFFDF
                     CKRLYGRNMNSRAIESLIKCGAFDGLGANRRQLLAISKTVLDDVEYESRRNAGGQMSF
                     FDDAEVDAQSSKPKIPDLPEFPVSELLHMENEIAGMYLSGHPLDEYTTFSKAIHADKT
                     GDIINNDSGMYFDGKKVSLVCIVAKLKTQLTKNNRIMAFVNAEDRYGMLEIVIFPNVY
                     EKYSALLGAGGALLFRGTVNLKENEEPKIICDSIASARTNEDCKNNPVAIKNTEHFSQ
                     NYQMQAVKNDTQKPSALYLRIDDLNTDKYNRAKRVLDIFDGRTPVIFYLTDTKRKVKA
                     PSSMWVDINPVMIKELKYQLGDENVVAK"
     gene            complement(7978..9192)
                     /gene="xseA"
                     /locus_tag="PLS64_05000"
     CDS             complement(7978..9192)
                     /gene="xseA"
                     /locus_tag="PLS64_05000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRL43221.1"
                     /translation="MYTPEFSKPRVLSVSQLNFYIKSIIENDARLNFVFVTGEISNLT
                     DHYRSGHIYLSIKDEKSVIRAVMFAGNARNLKFRPTDGMKVICRGRVAVYEPTGQYQL
                     YIEDMQPDGIGALSLAYEQLKKSLAEKGLFAPEHKKKIPPFPNSIGVITSPTGAAVQD
                     IRNILSRRFPSADIIMCPVLVQGENAPAQLIDAVQKLDKYNACDVIIIGRGGGSIEDL
                     WAFNDENLAYAIYNCKIPVISAVGHETDFTICDFVSDLRAPTPSAAAELAVPDTAELV
                     DYYKSQKQYVSSMATLKLRQEYNALTELKTRLSVVSPQQRIFEYEKSLELCKTKIQHF
                     VQETINKKAADITKTAAKLEGLNPVKILSRGYSIAEKDGKTVNSAKMLKSGDEFTLRF
                     SDGEIKAKATGE"
BASE COUNT         2488 a         1974 c         1910 g         2934 t
ORIGIN      
        1 ttgaatctac ccaagtatct gaaccgtctt gtttaacacg acctgcaaga taatatttaa
       61 cagaatcagt tactgtaaaa ctaacctcgg tgcttgtgac aggcgaataa ccatccgctg
      121 aaactgtgac cgtatacttg taagtgccgg ctacagtcgg ggttactgta agtttgttat
      181 tatcctgtac tacagtagca tcaccgccgg aagtcttagt aaatgtatat ttgagatttg
      241 tagaaatggg gttatcagct acagctgtga gagtaacaga atcacctact ttaacagttt
      301 cagatgaagc tgtaagtgaa gcgctatctg ctacagggtc agaatcaaaa tagtaagacc
      361 aagtaccatt actattatct atagcccaat ccttataagt ttcagttgtc atagtgtaat
      421 agttcatacc agaaggtata tctgaacaac ttttagaata gtcccactcc ttagtgtaat
      481 cattattata tctcaaaaat tgaacaacat tataagtagt attagaatca agctcaccct
      541 tgtatatagg tttgttttca tcaactttag tcatcgtaat tttatgccaa ttattgctac
      601 tatcctgaac atttaaccta aaatttgcac caccctcttg ccattttata ctaggtgtaa
      661 aataaatttc agaaagacca ctaacagtag cattaactgt tgaaacagtg ccgataagca
      721 ttgttgacag aagcatcatt accgcaagga tgacggaaac agtgcttctg cctaaatgag
      781 aaatttttgt tttcataaat attacctcca taaccgaata ttttggtttt ggttaggttc
      841 gggttcgacc tttaccgcct gaccaacggt gaaacataat cggcaaagcc acgggatttt
      901 gaagttaatt tacaaaaaaa caacgagaat tcagccgaaa attgtatcct taacctgttt
      961 cagtcaaaat gtggacacta acccatattc tttatagtag gttataccat tttagattga
     1021 ttttttcaat taggttgcaa taattttatt gtgtttgttt aatgtgatag ttttgtgttt
     1081 gtttttacat tcagttttgc cgttttgaca agatattttt atgttatgca caagttatgg
     1141 tacttttttt gtagaattcg tctatatatc ggtgttttta attattccga ttaagaaatt
     1201 gttatgccgt gtgttacaaa tttgataatg ctattttgct gaaagttcaa tatttgaatg
     1261 ttgtacagta acattataaa atcagnnnnn nnnnnnnnaa ttttccgcct ttgcgtgacg
     1321 gcagtccgac ggacacagct acgggtaacc gtgcatagtt taatatacaa ataaaaatgc
     1381 ccgactgcgg tgtcgggcat tttgtttgag attatatgat ttttgattat ttatccttac
     1441 ttaaaagcat acggtcgttt tcaagctttg aaccgcttgc tttttcaaac atctcgagga
     1501 ggtgttcaac cgtaagattt ttcttttctt cgcctgagat atcgaggata attttgccgt
     1561 cactcatcat aataagacgg ttgccgtgag taattgcgtc atgcatatta tgagttacca
     1621 taagtgccgt aaggttgttt tcggcaatga ttttgtcgga aagctcaagc acctttgcgg
     1681 ctgttttcgg gtcaagggct gctgtatgct cgtcaagcag gagaagcttt ggctttttga
     1741 ttgttgccat aaggagtgtt attgcctgac gctgaccgcc agagagaagt cctacttttg
     1801 atgacattct gtcctcaagt ccgaggtcga actctgcaag catctgacgg taacggattc
     1861 tttcgtcagc ctttacgccc cacgaaagtc ctcttttctt gcctcgtctt gcggcaattg
     1921 cgaggttttc tataatttcc atatcggcta ccgtgcctgt catcgggtcc tgaaatactc
     1981 tgccgaggta cggcgctctt ttatgctcgg gcaatgcgga aatatccttg ccgtcaatga
     2041 gaatctgacc gcagtcaaca ggccacacgc ctgcaattgc gttgagtgtt gttgacttgc
     2101 ctgcaccgtt accgccgatt accgtacaaa aatcgccgtc attgagggtg agattaactc
     2161 cgcagagtgc ctgcttttcg tttactgtgc ctttattaaa ggttttccat acattcttga
     2221 tttcaagcat tcttaaccct ccttctgtgc gtttttagca ttcatttttg caactttctt
     2281 gaatgaagtc tttctctttt tctggagata cggaactgca aggaagattg caacgataat
     2341 tgccgtaaag agctttgtat cgtctgtcgg catcttgagc cagagtacga tgcccattgc
     2401 gatataataa agaataccgc cgacaattac aaagaccatt ctgatgatga aattcatgtg
     2461 cttgcggaaa attgcctcac cgagaacttc accgatgatt acagcggcaa gaccgattac
     2521 gattgcgcct ctgcccatgt tgatatccgc aaagccctga tactgagcaa aaagtgaacc
     2581 cgaaagtgca acgagtccgt ttgaaagtgc aagagcaatt acctttgtaa aattgatgtt
     2641 aatgccctgt gccttgctca ttgcagggtt tgaacctgtt gaacggattg ttgaaccctg
     2701 ttccgtgccg aaataccaat acattgcggc aattacaacg gcacaaattc caagtcctac
     2761 gagaattgcc atatttatat tgcttgcaga aacaacaagc gtatagtttc tgtagctgat
     2821 tgggaggttt gattttccca taatattcag gttaattgaa taaagagcaa tctgtgtgag
     2881 gatacctgcg agaatgtcgg ggattccgag aagcgtgtga agaagtcccg ttacaagacc
     2941 tgccgcaaga cctgcaagga ttgctacgat gattgttgcc caaacgggac agccgttgat
     3001 gagcataact gcggttactg caccgcctgt tgcaaatgaa ccgtcaacgc tgaggtcggc
     3061 aaagttcaaa agcttaaagg tgagaaatac accgagtccc ataagtcccc agattatacc
     3121 ctgagcaata ttacccggaa gtgcggataa aaagctcatt ggattaagag aaccgaagta
     3181 gtttgctaaa aaatccataa aaaccaccta tacataaaat atcggggtgt cgcttgcacg
     3241 gcacccctgc caatattatt tttccgaata tgaattattc tgctttgatt tctgtgtaac
     3301 cgtcgggaac tttgattccg tatgtgtcac aacgatcttt gatatattct tttgttacat
     3361 tcggagcagc ctcaaccttc atctttgtga tgtcctcgcc ctttacaaga atgttgtaag
     3421 ccatttcgcc tgtttttctg ccgagttcat catagctgat tgaaagtgtt gcaacaccgc
     3481 aacccttgca gatgccttct tcgccggcaa caataggagt ttttgcggct gtggcaacat
     3541 tgtcgatgat acctgtgtta gatgccgctg tgttatctgt cgggatgtaa agaacatcgc
     3601 tctcgtcaca ggcagtcttt gcaactgcgg caacatcgtt tgaatctgca aatgaatatg
     3661 attttacagt atagcctgca tccttgaaat aaggagtgat tgtatcgcac tgatactttg
     3721 agttagcctc agccgaacag tagaggattc cgacattctt tgcatcgggg aaaagctcct
     3781 tgagcattgc cgcctgctga tcaagcggag caaggtctgt tgtacccgaa acattcatac
     3841 ccgaaacgcc gttccaatca gacttgtcaa gcgctgtgcc gtagtctgtg attgaagtac
     3901 cgagaatcgg aatattcggt gttgctgtgt atgctgcctg aagtgcggca gtaccgttgc
     3961 cgagaataag gtcatagttt gaagaaacaa actggttaca gattgtagcg caggttgcgg
     4021 aatcgccctg agcgttctgc tcgtcaaact ttacattgtc tgcaccgagt ttctcagtaa
     4081 gataatcttt aaagcctttt gttgcggcgt caagagcctc atgctgaaca agctgacaga
     4141 taccgatatt gtaaacctta ccgtccgtgg ctgaattatc tgcctttgag ctttctgcag
     4201 aaccgccgca tcctgcaaat gcgagtgaca tagaagccgc cgcaagagca actgcgacaa
     4261 tttttttagc ttttttcatt ttaatttcct cctaagcttt cagcactttc acgctgtaaa
     4321 gcatcatata cttctgcact tttatgcaat aaagtgtttt aacaacataa attataatac
     4381 aaaaaaacga aattttcaat agctttcacc aatttttatt gcaaataact gaaataaaag
     4441 tttgtttttg gttattttgc caccacattt tcgtcaccaa gctgatattt gagttctttt
     4501 atcattacag gattgatatc aacccacatt gacgaggggg ctttcacctt tcttttagtg
     4561 tcggtaagat agaaaataac aggcgttctg ccgtcaaaaa tgtcaagaac acgctttgca
     4621 cggttgtatt tgtcggtatt gaggtcatca atacgcaaat acaacgcaga aggcttttgt
     4681 gtatcatttt tcacggcttg catctgataa ttttgcgaaa aatgttctgt gttttttatt
     4741 gcaacagggt tatttttgca atcctcgttt gtacgggcag acgctatgga atcgcagatg
     4801 attttcggct cttcgttttc ttttaaattg acagttccac ggaacagcaa cgcaccgcct
     4861 gcaccgagaa gggcggaata tttttcatat acattcggga aaatcacgat ttcaagcatt
     4921 ccgtatctgt cctcggcatt gacaaaagcc ataatcctgt tattttttgt aagctgggtt
     4981 ttcagctttg caactatgca gacaaggctg actttttttc cgtcaaaata cataccgctg
     5041 tcattattga ttatatcgcc cgttttgtcg gcatggattg cttttgaaaa agttgtgtat
     5101 tcatcgagcg gatgaccgct gagatacatt cccgcaattt cgttttccat atgcaaaagt
     5161 tcagaaacgg gaaattcggg caaatcggga attttgggct ttgatgattg agcatcaacc
     5221 tctgcgtcat caaaaaacga catctgaccg cccgcatttc ttctgctttc atactcaaca
     5281 tcatcaagaa cggttttact gattgcgagc aattgtctgc gatttgctcc gagtccgtca
     5341 aatgcaccgc attttatcag actttcaatt gctctgctgt tcatatttct gccgtagagc
     5401 cttttgcaga aatcgaagaa atcttcataa ggattgttta tgcgctccga gatgatttca
     5461 tctatgaaat ttctgccgag atttttaacg gcaagaagtc cgtaacggat atcgtttccg
     5521 ctgaccgtaa aatgaagatt gctttcgttg acattaggcg gcaacacacg gattccgagc
     5581 cgtttgcact cagtgatata tgacgcaagt ttattttgat tatcaagaac acttgaaagc
     5641 agagccgcca tatattcacg gggatagtgg catttgagcc atgcagtttt gtaggaaatg
     5701 ttggcatatg ctgcagcatg ggatttgtta aatgcgtagt gtgcaaagct ttccatttca
     5761 tcataaacgg aaattgccgt tttttcatca accccacggc gaatgcatcc ttcaactatg
     5821 atatttccgt catcatcagt aagaccgtga atgaatattt cacgctccga ctccattacg
     5881 gcaagttttt tcttgctcat tgcacggcgc actatgtccg ctctgccgag actgtagcct
     5941 gcaagcgttc tgaaaatctg cataacctgt tcctgataaa ttatgcaacc gcttgtaact
     6001 ccgagaattt cctttaaaag cggatgtttg taacgaatat gcgacggatt gtgacgacag
     6061 tcaatataaa cgggaatgct gtccataggt ccgggacgat agagagccgt aacagctata
     6121 agatcctcaa ttcgctccgg ttttagctga gtgagaacat ttttcattcc ttggctttca
     6181 aactgaaaca caccctccgt gtatcctttt gaaaacattt caaaaacttt tttatcgttt
     6241 tcatcgacca tatcgggatt atagtcggga tttttatgtt ttatctgctt ttctgcatca
     6301 ttgattacgg ttaagtttct gagtccgagg aagtccattt ttaaaagacc gagttcttca
     6361 agtgccgtca tggtgaactg tgttacaaca ttatcgtcat tttttgacag cggaacatag
     6421 tcgctgacgg gcttatcggt gattacaact cctgcggcgt gcattgttgc gtgacggggc
     6481 attccctcaa tgctcattgc aacatcaagc agttttttta tctgcggatc gttattgcag
     6541 cgacttctca gatcgggact cagtttcagc gcttcctcga ttgtcatatc atgttccata
     6601 ggaacaagct ttgcaacaga atctactgtc gcatacggaa tttcaagcac ccttccgaca
     6661 tctcggattg cacctcttgc cgccattgta ccgaacgaaa ttatctgtgc aacatgatct
     6721 tcgccgtatt tacgcacaac atagtcgata acctctcccc tgcgttcctt acaaaaatcg
     6781 acatcaaaat cgggcatact gactctttcg ggattgagaa atctttcaaa aagaagattg
     6841 tactttatcg gatcgattgc ggttatgcct atgcagtagg cacaaagtga gcctgcaccc
     6901 gagcctctgc cgggtccgac aggaatttgg catgattttg cgtagcttac aaagtcattt
     6961 acaatgaggt agtaatctac aaaacccatt tgcgacaccg tgttgatttc atattcaagt
     7021 cggtcaatca gagctttgtc gggattctcg ccatagtgtt tataaagccc ctcatagcat
     7081 tttcttctga aataatgcag atgatcttca ttattaggaa catcaaatct cggaagcttg
     7141 cgtacaccga attcaaaatc cacattgcac atatcggcaa ttttctgtgt attgtcgatt
     7201 gcttcggggt aacttgcaaa cacctcacgc atttcatctt ctgttttaag ataaaattcc
     7261 gaagttttga actccattct gtcgctgtca ttgactgttc ggttggtctg aatacagaga
     7321 agaatattgt gaaccgtgct gtcctgtttt tcgatatagt gacaatcgtt tgttgcaaca
     7381 atgtcaacac cgatttcacg ggcaattttt ataaggcttg gaattatcct tttctgttcc
     7441 tcaattccgt ggtcttgaag ctctataaaa aagttctcac tcccgaatat atcacggtaa
     7501 aattctgcct ttgactttgc accggcataa tcaccgtcaa ggagcttttg aggaatttca
     7561 cccgccagac aggcagagag gcagactaag ccctcatgat actcggcaag gagttcatca
     7621 tcaattctcg gctttgtata gaatccctcc gtaaaaccct ttgacacaag cttgataaga
     7681 ttctgatagc ctgtatgatt tttgcagagc agaaccatat gatagtattt tccgtaggat
     7741 gaggtcttgt caaaacggct ttgcggtgca acatacactt cacagccgat tatcggcttt
     7801 ataccctgct ttttgcattc cttataaaaa tcgactgcac cgtacattac accgtggtcg
     7861 gtgattgcga gtgcagtcat tcctttttct tttgcggctt ttgcaagtct gctcagacgg
     7921 caggcaccgt caagcagact gtattctgta tgaagatgta aatgtgtaaa tgccatttta
     7981 ctcacctgtt gcttttgcct ttatttcacc gtctgaaaaa cggagtgtaa attcatctcc
     8041 gcttttcagc atttttgcgg aatttaccgt cttaccgtct ttttcggcaa ttgaataacc
     8101 tcttgaaaga attttaacgg ggttgagtcc ttcaagtttt gcggctgtct ttgtgatgtc
     8161 ggcagccttt ttatttattg tttcctgaac aaaatgctgt attttcgttt tgcaaagttc
     8221 aagagatttt tcatattcaa aaattctctg ttgtggtgat acaacggaca gtcttgtttt
     8281 taattctgta agagcattat attcctgtct taatttcaat gttgccattg acgaaacata
     8341 ctgcttttgc gatttatagt aatcaacaag ctccgcagta tcgggaactg caagctctgc
     8401 cgccgcagac ggagtcggcg ctctgagatc ggatacaaag tcacagattg taaaatcggt
     8461 ttcatgaccg acagcggaaa ttacgggaat tttacagttg tagattgcat aagcaaggtt
     8521 ttcatcattg aatgcccaca aatcttcaat tgaaccgccg cccctgccga ttatgataac
     8581 atcacaggcg ttgtatttat caagtttctg tacggcgtca ataagttgag cgggagcatt
     8641 ctcgccctga acaagtacgg gacacataat tatgtcggca gacggaaatc ttcttgaaag
     8701 aatgttgcgg atatcctgaa ctgccgcacc tgtgggagaa gtaattacac cgattgaatt
     8761 cggaaacggc ggaattttct tcttatgttc gggagcaaaa agtccttttt cggcaagtga
     8821 ctttttaagt tgttcatatg caagcgaaag cgcacctatg ccgtcgggct gcatatcttc
     8881 aatataaagc tgatactgac ctgtgggttc atagactgca actctgccac ggcagattac
     8941 tttcattccg tcggtcggtc tgaactttaa attacgggca ttgccggcaa acattacggc
     9001 tcttataacg gatttttcgt ctttgattga aaggtagatg tgtccgcttc tgtagtggtc
     9061 ggttaggttt gaaatttcac ccgtaacaaa gacaaaattc agccttgcgt cattttcaat
     9121 aattgacttt atatagaaat tcagttgtga aacacttaaa actcgtggtt tgctaaactc
     9181 gggtgtgtac atcgttatac ttcctgtatc ctaaatatgc aattcctgcc gagttatctg
     9241 ccgaaaactg cggcggtgcg aatattgcat tatatttttc ttcaaaagaa tttttgatta
     9301 tccgatttga cattactcc
//
LOCUS       DAOMVE010000065         9230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1894_length_9230_cov_2.928828, whole genome shotgun sequence.
ACCESSION   DAOMVE010000065 DAOMVE010000000
VERSION     DAOMVE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9230
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1894_length_9230_cov_2.928828"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /gene="guaA"
                     /locus_tag="PLS64_05005"
     CDS             complement(<1..1525)
                     /gene="guaA"
                     /locus_tag="PLS64_05005"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRL43222.1"
                     /translation="MSNATKETVIVLDFGGQYNQLIARRVRECNVYCEILPYTVDIQK
                     IKDINPKGIIFTGGPNSVYDESSPHYTPEIFELGIPILGICYGCQLMAYTLGGNVVSA
                     TDNSSSEYGKTVTRYNNNDILFKSVKSEGISWMSHRDYINEAPEGFSITATTDVCPVA
                     AMSCPEKKFYGVQFHPEVNHTDNGKDMLRSFLYDVCECKGEWKMESFIDTTVAQLKEQ
                     IGDKGVVLGLSGGVDSSVAAALLSKAVGKQLTCVFVDQGLMRKDEGDFVEQTFTKLFD
                     MNFVRINCQEEFLAKLKGVEEPEEKRHIIGTEFYKVFWNKIRESYGEGYFAQGTIYPD
                     RIESGKGDAAKIKTHHNQVGIPEDIDFAGVIEPLKDLFKDEVRVVGEKLGLPHDLVWR
                     QPFPGPGLGVRVIGEVTAEKVRILQEADAILRDEMDKCGYADKMSQFFAVLPGVKTVG
                     VMGDARTYDELIALRAVTTDDFMTADWAKIPYEILGRVSNRIINEVEHVNRVVYDITS
                     "
     gene            complement(1593..2870)
                     /locus_tag="PLS64_05010"
     CDS             complement(1593..2870)
                     /locus_tag="PLS64_05010"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRL43223.1"
                     /translation="MVKAIVGANWGDEGKGKITDVLANDSDIVIRFQGGANAGHTIVN
                     DYGKFALHQLPSGVFHQNITNIIGNGVAFSVENFVKEMAHLKDRGVPEPNIIISDRAQ
                     IVMPYHVAFDCYEEERLGKNSFGSTKSGIAPFYSDKYSKIGFQINELYDDPDSLREKI
                     RHALEIKNATLKNLYNKPEITEQEVFDKLMEYKELLAPYVGDAFTFVHNALKDGKNIL
                     LEGQLGSLKDTDFGIYPMVTSSNTIAGYGAVGAGIPPYEIKEIITVVKAYSSAVGAGE
                     FVSEIFGDEADELRRRGGDGGEFGATTGRPRRMGWLDLVATRYGCMVQGATQVAFTVL
                     DVLGYLDEIPVCVGYELDGKEIDYFPSTTKLKRCKPILKKLKGWKCSISGITEYDKLP
                     KECREYIEFAEKAIGVPITMVSNGPSRNDIIYR"
     gene            complement(2955..4391)
                     /gene="purB"
                     /locus_tag="PLS64_05015"
     CDS             complement(2955..4391)
                     /gene="purB"
                     /locus_tag="PLS64_05015"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HRL43224.1"
                     /translation="MVKDTYESPLSARYASKEMKYIFSPDKKFRTWRKLWIALAESEK
                     ELGLPITQEQIDELKAHADDINYEVAQEREKIVRHDVMSHVYAYGVQCPNAKGIIHLG
                     ATSCYVGDNTDIIIMTEGLKLIRNKLITVIRNLSKFADEYKALPTLAFTHFQPAQPTT
                     VGKRATLWLQELLMDLEDVEYQLSKAKLLGSKGTTGTQASFLELFDGDHEKCKMLDRK
                     IAEKMGYKACFPVSGQTYSRKLDSQFLNVLAGIAQSAAKFSNDIRLLQHLKEVEEPFE
                     KNQIGSSAMAYKRNPMRSERIGSLSRYVMVDVLNGYFTTATQWFERTLDDSANKRLSV
                     PEAFLAVDGILSLYANVADGLVVYPKVIEQRLRKELPFMATENIMMDAVKKRGADRQQ
                     LHEKIREHSMAASRVVKVEGGENDLLERIAADEAFGVTLEELEKILKPENYTGRAKEQ
                     TEDFLNECIKPVLEKYADVESDKPEINV"
     gene            complement(4409..5875)
                     /gene="purF"
                     /locus_tag="PLS64_05020"
     CDS             complement(4409..5875)
                     /gene="purF"
                     /locus_tag="PLS64_05020"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRL43225.1"
                     /translation="MEFSFDNFAKEGLHEECGVFGIFSDGTINPAYACYNGLLALQHR
                     GQESCGIAVSDEGVIDYHKDMGLVTEVFNNKILDSLNGQMAVSHVRYSTAGGSVLENA
                     QPLVMRYVKGTLSIAHNGNLTNALEIRRSLEQRGAIFQTTIDSEVICYVIARERLKCH
                     SVEDAVARTMQVIEGAYSLLVMSPRKLIAARDPHGFRPLCMGKYNDSYVFASESAALD
                     ACGAEFVRDVEPGEIVIVDQNGVRSMKPDFGDKKKALCIFEYIYFARSDSFIDGVSVY
                     EARKQAGRILARKYPVEADMVVGVPESGIDAAIGYSEESGIPYEKAIVKNSYIGRTFI
                     KPSQKERMKSVKIKLNPIADKIKGKRVVIIDDSIVRGTTVDRIVTMLKEAGAKEVHMR
                     ISSPPFMWPCYYGTDIPSRGELIACNHTIEEITKLSKADSLGYLPVESLREMIHNAPV
                     GFCDGCFTGNYPAPITKETFKGKERGGMNFDENNKKDN"
     gene            complement(5956..6663)
                     /locus_tag="PLS64_05025"
     CDS             complement(5956..6663)
                     /locus_tag="PLS64_05025"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRL43226.1"
                     /translation="MEKKELLYEGKAKKVFSTEDPDLCIVSYKDDATAFNGEKKGTIV
                     GKGVVNNRMSNFMFKLLEKHGIATDFVEELNDRDTVLKKVEIVPLEVILRNKAAGSLS
                     KRLGLPEGTPMKTPVLEFCYKNDELGDPMVNEYHILAAGFATKEEIDTITEMAFKINE
                     IMIEFFKQCKVDLIDFKIEFGRYKGKILLADEISPDTCRFWDMDTQEKLDKDRFRRDM
                     GGVEEAYAEMMKRVGLA"
     gene            complement(6895..7398)
                     /locus_tag="PLS64_05030"
     CDS             complement(6895..7398)
                     /locus_tag="PLS64_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43227.1"
                     /translation="MGGISVMLLMGMLSAMYVFFIAVIAVLIIYFAVSYVFESIAAMG
                     MCKSLYYKHSGTAWIPFYNKYLLGKIAGNKALGIIEGVLNLSIATVAVLFFCFELEIG
                     IIGLIALLACVIASFVIDVIIADKIFKNLNIRYSDVLTVVNVLTFGLLRPIILFIFRN
                     KQNTVRF"
     gene            complement(7459..7545)
                     /locus_tag="PLS64_05035"
     tRNA            complement(7459..7545)
                     /locus_tag="PLS64_05035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7509..7511),aa:Leu,seq:caa)
     gene            7827..8882
                     /locus_tag="PLS64_05040"
     CDS             7827..8882
                     /locus_tag="PLS64_05040"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRL43228.1"
                     /translation="MINNQFSGNNMPFYGNNLNYNRNFKKDMQYDNLKQRSSGLGFFV
                     FAYSLTMTVSAMIVVEVFKAFGSAKYIYSDNYSILTYFMDIFISVFAVVVPAFFYIKL
                     SRNSLNDIISTKYVKQKSMIPILFLGMGGAMIANVATSVIADNFSLFGIENTSSGLTI
                     GDSSVLSIVLNIISTAIVPAFAEEFAFRGIVMGSLRKFGDTTAVIGSAILFGAMHQNI
                     SQIPFAFILGLIFAYVDCKFNSILPSIAIHFLNNLYAVSMSILRDANIVSDYAATIIS
                     YSLIIFFCIAGFVSYIVLAKNDKNFFKMSDKKDGIVSELSLKEKMTAFLVNPGIILSL
                     VLFLITTITNLGLINNG"
     gene            8875..>9230
                     /locus_tag="PLS64_05045"
     CDS             8875..>9230
                     /locus_tag="PLS64_05045"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HRL43229.1"
                     /translation="MDKTLYYMQKAIEQAEIAAAENEVPVGAVIVRNDEIVATGRNRR
                     EQGKNALLHAETDAIYNACQKLGGWRLWNCEIYVTLEPCPMCAGAIINAHIPKVYFGA
                     YDLKNGACGTITNLFQ"
BASE COUNT         2612 a         1992 c         1807 g         2819 t
ORIGIN      
        1 tacttgttat atcatagact actctgttga catgttctac ttcatttatg atgcggttgg
       61 aaactctgcc taggatttcg taggggattt ttgcccagtc ggctgtcata aaatcatcgg
      121 ttgtaacggc acggagggcg atgagttcgt catatgttct tgcgtcaccc ataacgccta
      181 cggttttaac acccgggagg actgcgaaga actggctcat tttatctgcg taaccgcact
      241 tatccatttc atcacgaaga attgcatcgg cttcctgaag aatacgaacc ttttcggctg
      301 taacttcacc gattacacgc acaccgagtc ccggacccgg gaacggctga cgccatacga
      361 ggtcgtgcgg aagtccaagc ttctcaccta ctactcttac ctcatccttg aagaggtctt
      421 taagcggttc aattacacct gcaaagtcaa tatcttccgg gataccaacc tgattgtgat
      481 gtgttttgat ttttgcggca tcgcctttac cgctttcgat acggtcggga taaattgtac
      541 cctgtgcaaa gtaaccctcg ccgtagcttt cacggatttt gttccagaac accttataaa
      601 attctgtgcc gatgatgtgg cgcttttctt cgggttcttc aacaccttta agctttgcaa
      661 gaaactcttc ctgacaatta atgcggacaa agttcatatc aaaaagcttt gtaaaggtct
      721 gctctacaaa gtcgccctcg tcttttctca taagtccctg gtccacgaaa acgcaggtaa
      781 gttgtttgcc aactgcctta ctgagaagtg ctgcggcaac agatgaatct acaccgcccg
      841 aaagtccgag aactacaccc ttatcaccaa tctgctcttt aagctgtgca acagttgtgt
      901 cgatgaagct ttccattttc cactcaccct tacattcgca aacatcataa aggaatgaac
      961 ggagcatatc tttaccgtta tctgtatggt taacttcagg atggaactga acaccgtaaa
     1021 actttttttc aggacatgac attgcggcta cgggacatac atccgttgta gctgtaatgc
     1081 tgaatccttc gggagcttca ttgatatagt cacggtgact catccatgaa attccttcac
     1141 tctttacgga tttaaagagt atatcattgt tattatatct tgtaactgtc ttgccgtact
     1201 cacttgatga attgtctgta gccgaaacaa cattgcctcc gagcgtgtat gccataagct
     1261 gacaaccgta gcaaatgccg agaatcggga ttccgagttc aaaaatttca ggagtatagt
     1321 gcggtgatga ctcgtcataa acgctgttag gaccgcctgt gaagataata ccttttgggt
     1381 taatgtcttt gattttctga atatcaacgg tataaggaag aatttcgcaa tacacattgc
     1441 actcacgaac tcttcttgca atcagctgat tgtactgacc gccgaaatca agaacaatga
     1501 cagtttcttt tgtagcattg ctcatagtaa aattcctttc aaatttaaag aataccgcac
     1561 agagtttgtg cggtattcaa ttaatatttt aattatctgt aaataatatc gtttctggaa
     1621 ggaccgtttg aaaccattgt aatcggcaca ccgatagcct tttcggcaaa ttcaatgtat
     1681 tcacggcatt ccttaggaag cttgtcatat tctgtaatac ctgaaattga acacttccag
     1741 cccttgagct ttttgaggat aggtttgcat cttttaagct ttgttgttga cgggaagtag
     1801 tcaatttcct tgccgtcaag ctcataacct acacaaacag ggatttcatc aagatatccg
     1861 agaacatcaa gaaccgtgaa agcaacctgt gttgcaccct gaaccataca accgtagcgt
     1921 gttgcaacga ggtcaagcca acccattctt ctcggtctgc ctgttgttgc accgaattct
     1981 ccgccgtcac cgcctcttct gcgaagttcg tcagcctcat cgccgaagat ttcagaaaca
     2041 aattcacctg cgcctaccgc acttgaataa gccttgacaa ctgtgatgat ttctttgatt
     2101 tcatacggag gaatacctgc accaacagca ccgtaacctg cgattgtatt ggatgaagta
     2161 accataggat aaataccgaa gtcagtatcc ttgagtgaac cgagctgtcc ttcaagaaga
     2221 atattctttc cgtctttgag cgcattatga acaaaagtga atgcgtcacc gacataagga
     2281 gcaagaagtt ccttatattc cattagcttg tcaaaaactt cctgttctgt gatttcgggc
     2341 ttgttgtaga ggtttttcag agttgcattc ttgatttcaa gagcgtgtct gattttctct
     2401 ctgagtgaat cgggatcatc ataaagttca ttaatctgga aaccgatttt tgaatacttg
     2461 tcagaataga acggagcgat accgctcttt gttgaaccga aagaattttt gccgagacgc
     2521 tcttcctcat agcagtcaaa tgctacatgg taaggcataa caatttgtgc tctgtcagaa
     2581 ataataatgt ttggctcggg aactccgcga tctttgaggt gagccatttc cttaacgaag
     2641 ttttcaacgc tgaaagcaac accgttgccg attatattgg tgatattctg atggaaaaca
     2701 cccgaaggaa gctggtgaag agcaaatttt ccgtaatcgt tgacaattgt atgtcctgcg
     2761 tttgcaccgc cttgaaaacg aattacgatg tcgctgtcgt ttgcaagaac atcggtaatt
     2821 ttacccttgc cctcgtcgcc ccagttggca cctacaattg cttttaccat tttacaaaac
     2881 tcctttttac agacgcatgg tctgctataa ataaaatata tacttgaacc aaaggttcga
     2941 ttaaatcagt ctaattaaac attgatttca ggcttgtcag actcaacatc tgcgtacttt
     3001 tcaagaacag gcttgatgca ctcgttgagg aagtcctctg tctgctcttt tgctctgcct
     3061 gtgtagtttt ccggcttgag aatcttttca agctcttcaa gagtaacgcc gaatgcctca
     3121 tctgcggcaa ttctctcgag aaggtcattc tcgccgcctt caaccttgac tactcttgac
     3181 gcagccattg aatgctcacg gattttctcg tgaagctgct gtctgtcagc accacgcttt
     3241 ttaactgcat ccatcataat attctctgtt gccatgaacg gaagttcttt tctgagtctt
     3301 tgttcaatta cctttggata aacaacaaga ccgtctgcaa catttgcata aagtgaaagg
     3361 ataccgtcaa ctgcgaggaa tgcctccgga acgctgagac gcttgttggc ggagtcatca
     3421 agtgttcgtt caaaccactg tgtggcagtt gtaaagtaac cgttgagaac atcaaccata
     3481 acatatcttg aaagtgaacc tatacgctca cttctcattg ggttacgctt gtaagccata
     3541 gccgatgaac cgatctgatt cttttcaaac ggctcttcaa cctctttgag gtgctgaaga
     3601 agtctgatat catttgagaa tttagcggct gactgagcaa ttcccgcaag aacattgagg
     3661 aactgtgagt caagctttct tgaatatgtc tgacctgata cagggaaaca agccttgtag
     3721 cccatttttt ctgcaatctt acggtcaagc attttgcact tttcgtgatc tccgtcaaag
     3781 agttcaagga aacttgcctg tgtacctgtt gtaccctttg aaccgagaag cttagccttt
     3841 gaaagctgat actcaacatc ttcaagatcc ataaggagct cctgaagcca gagagtcgct
     3901 ctcttaccta ctgttgtagg ctgtgcaggc tgaaaatgag tgaatgcaag ggttggaaga
     3961 gccttgtatt catcggcaaa ctttgaaagg ttgcggataa cggtgataag cttatttctg
     4021 ataagcttga gtccttctgt cataatgatg atatctgtgt tatcaccgac atagcaggag
     4081 gttgctccga gatgaataat gccctttgcg ttcggacact gaacaccgta agcatataca
     4141 tgtgacatta catcgtgacg aacaattttc tctctttcct gtgcaacttc atagttgatg
     4201 tcatcagcat gagccttgag ttcgtcaatc tgctcctgtg taattgggag tccgagttct
     4261 ttttccgatt ctgcaagagc aatccagagt tttctccatg ttctgaattt tttatccggc
     4321 gagaaaatgt atttcatttc cttgcttgca taacgggctg acaagggtga ttcataagta
     4381 tcttttacca ttatttacat cctttcaatt agttgtcctt tttattgttt tcatcgaagt
     4441 tcattccgcc gcgctctttg cccttaaagg tttcttttgt gataggagcg gggtaattac
     4501 ccgtaaagca accgtcacaa aagcctacgg gagcgttgtg aatcatctca cgaagtgact
     4561 caacagggag gtatccgagg gaatcagcct tgctgagctt tgtgatttct tcgattgtat
     4621 ggttgcaggc aataagttct cctcttgacg gaatgtctgt accgtaataa cacggccaca
     4681 taaacggagg tgaactgata cgcatatgta cctcttttgc gcctgcctct ttgagcattg
     4741 taacaattcg gtcaacagtt gtaccacgca caatgctgtc atcaataata acaactctct
     4801 tgcctttgat tttgtcggca atcgggttga gtttaatttt tacgcttttc attcgttcct
     4861 tctgacttgg ctttatgaag gttctgccga tgtagctgtt cttcacaata gccttttcat
     4921 aaggaattcc ggattcttcg ctgtaaccga ttgcggcatc aattcccgat tcgggaacac
     4981 cgacaaccat atcagcctca acagggtatt tccttgcaag aattcgtcct gcctgctttc
     5041 ttgcctcata aacgcttact ccgtcaataa aggagtctga acgggcaaaa taaatatact
     5101 caaaaatgca aagtgccttt ttcttgtcac caaagtcggg tttcatactg cggacaccgt
     5161 tttggtctac aattacgatt tcacccggtt caacatcacg gacaaattct gcaccgcagg
     5221 catcaagagc cgcactttca cttgcaaaaa cataggaatc gttgtacttg cccatacaaa
     5281 gcggtctgaa tccgtgagga tcacgagccg caataagctt tctcgggctc ataacaagga
     5341 gcgaataagc tccctcaatt acctgcatcg ttcttgcaac ggcatcttca acagaatgac
     5401 atttaagtct ttcacgggca attacatagc agataacctc gctgtcgatt gttgtctgaa
     5461 agattgcgcc gcgctgttca agacttctgc ggatttcaag cgcattggta aggttgccgt
     5521 tatgggcaat tgaaagcgta ccctttacat aacgcataac aagcggttgt gcattttcca
     5581 gcacagaacc gcctgcagtc gagtaacgga catgcgaaac cgccatttgt ccgttgagag
     5641 aatcaagaat tttgttgtta aagacctcgg taacaaggcc catatctttg tgataatcaa
     5701 ttacaccttc gtcagataca gcgatgccgc agctttcctg tcctctgtgc tggagagcaa
     5761 gcaagccgtt ataacaagcg tatgcagggt taattgtgcc gtcgctgaat atgccgaata
     5821 ctccgcactc ttcgtgcaga ccttcttttg caaaattatc gaaagaaaat tccaaggtgc
     5881 caccatccga ttaatcaaaa taactatcca aagtttaatt cacatatctt aattcaaaaa
     5941 ttcacaatgt agttattatg caagacctac acgcttcatc atttcggcat aagcctcttc
     6001 aacaccgccc atgtcacgac ggaaacggtc tttgtcgagt ttttcctgag tatccatatc
     6061 ccagaaacgg catgtatcgg gagaaatttc gtcagcaagg agaatcttgc ccttatatct
     6121 gccgaactca atcttgaagt cgataagatc aaccttgcac tgcttgaaga attcaatcat
     6181 aatctcatta atcttgaaag ccatttctgt gattgtgtcg atttcttcct ttgtagcaaa
     6241 gcctgctgcg aggatgtgat attcatttac cataggatcg ccgagttcat cgttcttata
     6301 gcagaattca agaacaggag tcttcatcgg tgtgccttcg ggaagaccga gacgctttga
     6361 aagactgccg gcagctttat ttcttaaaat tacctcgaga ggaacaattt caaccttctt
     6421 gaggactgta tctctgtcgt taagctcttc aacaaagtct gttgcaatgc catgcttttc
     6481 aagaagcttg aacataaagt ttgacatacg gttgtttaca acacccttgc caacgattgt
     6541 accctttttc tcgccgttga atgcagttgc gtcatccttg taggaaacga tgcagagatc
     6601 gggatcttct gtagaaaata cctttttagc ttttccttcg taaagtaatt ctttcttttc
     6661 cataatcaaa tcctccataa agatgcggct ttgcgccgta attaaaaaac aatacaagat
     6721 acattataat atataaatca aaaataagca accgtatgag aattaaaaat aaaaaaattg
     6781 acgaattttt taaaaaattg cctgtatcaa aaaattatga aacaaaaata atcttaaaca
     6841 gggcattttt gcaaaaggaa aaggaacaat acggcgtgta ttgctcctta gaaattaaaa
     6901 tctgactgta ttctgtttat ttctgaatat aaaaagaatt ataggtctta aaagtccgaa
     6961 ggtaaggaca tttactactg tgagaacatc cgaatatctg atgttaagat ttttgaatat
     7021 tttatcggcg ataattacat ctatcacaaa tgaggcaatg acacaagcta acagggcaat
     7081 caagccgatg ataccgattt ccagttcaaa gcagaaaaac agaacggcaa cggttgcgat
     7141 tgaaaggttc agcacacctt ctattatgcc taaagcctta ttccctgcaa ttttaccgag
     7201 gagatattta ttataaaacg gaatccaagc tgtaccgctg tgcttatagt acaagctctt
     7261 gcacataccc attgccgcaa tgctctcaaa aacatagctt actgcaaaat aaataatcag
     7321 cacggcaatt actgctataa agaacacata catagccgaa agcattccca ttaaaagcat
     7381 aacgcttatt ccgcccataa ttatcgccct ccgaaatata ctaaaaaagg acactaactc
     7441 tatgagttaa tgtccttttg gtgccggtga ccggacttga accggtacgg tatcgctacc
     7501 ggcggatttt gagtccgcta cgtctgccaa ttccatcaca ccggcaaatg caaaacatat
     7561 tatacaatac aaatcaggaa aaatcaagtt atatttttaa attttttgcc ttaattacta
     7621 cgctgtaaat agaggtgaat caatttgtgt gaaaatacaa acggtttttc ttctttggcg
     7681 tcgattttgg gtgaatttaa tactgtattt taaagttact ttttgtcgga ttacatcatt
     7741 ttaatcatgc aaatacttta tacggatgtt ttaatccttg aaaataatat gcctctatgt
     7801 tagaatagct ttgaggatgt gataatttga taaataacca gttttcgggt aataatatgc
     7861 cgttttacgg caataacctt aattataacc gcaatttcaa aaaagatatg cagtatgata
     7921 atctcaagca aagatcaagc ggtttaggtt tttttgtatt tgcgtattcg cttacaatga
     7981 cagtaagcgc aatgatagtt gtagaagttt tcaaggcttt tggttcggca aaatatattt
     8041 attctgacaa ttattcaata ctgacttact ttatggatat atttatttcc gtatttgccg
     8101 ttgttgttcc tgcatttttt tatataaagc tttcaagaaa cagtttaaat gatattatca
     8161 gcacaaaata tgtaaaacaa aaaagtatga tacccattct gtttctcgga atgggaggtg
     8221 caatgattgc aaatgttgcc acgagtgtta tcgcagacaa cttctcgctt ttcggaattg
     8281 aaaacacatc ttcgggattg acaatcggag attcaagtgt tttgagtatt gttctgaaca
     8341 taatttcaac agcaatcgtc cccgcttttg ccgaagaatt tgctttcaga ggaatagtta
     8401 tgggttctct gagaaagttc ggtgacacaa cagcagttat aggctctgca attttgttcg
     8461 gtgcaatgca tcagaatata tcacaaattc cgtttgcgtt tattctcgga cttatttttg
     8521 cgtatgtcga ttgcaaattc aattccattc ttccgtcaat tgcaatccat tttcttaata
     8581 acctatatgc tgtttccatg agcattttaa gagatgcaaa cattgtttcc gattatgcgg
     8641 caacaataat cagttacagc ctgattattt tcttctgtat tgcaggcttt gtttcatata
     8701 tcgtacttgc caaaaatgat aagaactttt tcaaaatgtc tgataagaaa gacggaattg
     8761 taagtgaact tagcttaaaa gagaaaatga ctgcatttct tgtgaatccc ggtatcatac
     8821 tttcacttgt actttttctg ataactacaa tcacaaattt aggattaatt aacaatggat
     8881 aaaactttat actatatgca aaaggctatt gaacaggctg agattgccgc tgcggaaaac
     8941 gaagttcccg tcggtgcggt aattgtcaga aatgatgaaa ttgttgccac aggaagaaat
     9001 cgccgtgaac aaggcaagaa cgctcttctt catgccgaaa ccgacgcaat atacaatgcc
     9061 tgtcaaaagc ttggcggttg gagattgtgg aactgtgaaa tttatgtaac acttgagcct
     9121 tgccctatgt gtgccggtgc aataatcaac gctcatattc caaaggtata tttcggtgct
     9181 tacgacttaa aaaacggtgc ttgcggtaca attacgaatc tgttccaaat
//
LOCUS       DAOMVE010000066         9033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_1963_length_9033_cov_3.947984, whole genome shotgun sequence.
ACCESSION   DAOMVE010000066 DAOMVE010000000
VERSION     DAOMVE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9033
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1963_length_9033_cov_3.947984"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1171)
                     /locus_tag="PLS64_05050"
     CDS             complement(149..1171)
                     /locus_tag="PLS64_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRL43230.1"
                     /translation="MIQLATIRDVAKKANVSVATVSRVLNNDTTLSTSLETKQKIFAA
                     AKELNYVKKKRVTSAPSCRIGILQWFSSKQEIEDNYYFLMRQGIEDYCSKNNISIVRT
                     FKTDNDYQNVLCDVDGIICLGKFSKQEIKLLHSLNNKIVFLDMTVENIEITSVSLDFR
                     QAVSDAIKYLYDLGHRTVGFIGGIEQLEDGTIFPDKRLNTFTDICDELNIKYDGFIIQ
                     DKFSTSSGYKMMSEMISKGNLPTAIFAASDHIAIGAMRALQENGYKIPEDISVMGFDN
                     TELSGYTNPPLTTVNAPVYAMGIYGIRFILNLMKPKSTDYYSPMRVYLPCPIKVRNSC
                     AKPKSL"
     gene            1391..2866
                     /gene="melB"
                     /locus_tag="PLS64_05055"
     CDS             1391..2866
                     /gene="melB"
                     /locus_tag="PLS64_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HRL43231.1"
                     /translation="MKQTSSQPRLDGKIPGRIKYTFALGALGKDMIYGMIATFSMIYF
                     TDIIKVNPVFIGVMFFVAKLWDAFNDLFMGMIVDNTRSRWGKFIPWLVIGTLVNSIVF
                     VVLFTDFHLDGVGLCVFATAAYILWGMTYTIMDIPYWSIIPNLTSNPEEREKVSVLPR
                     IFASIGQSLVIAGFGVQIIEALSGGTTNQAGYHRFAIIIAIVFILTIGITVINLPKKR
                     DDSVPEKKVKFKDIFSIIGKNDQLRWAVTLIFLYNIGIQCIMGVATYYFKYVCGNAGM
                     MSSFMISASIAEVVGLIVFPKVTKFLSRKTSFLLACLLPAFGLVMLLFVGIFAPHNVV
                     LTSISGVIVKLGTGLELGCATVFLADVVDYGEFKLGTRNEGVVFSLQTLIVKFTAALT
                     ALGIGAALGSTQYIPGQEQSFATVASISTLMCIVPAVCMLIAYVVYRKKYKLNDGMMK
                     KVINTISARREGKIDMTQQLDESGNTEPSMFLPTVAEKAGK"
     gene            2868..4148
                     /locus_tag="PLS64_05060"
     CDS             2868..4148
                     /locus_tag="PLS64_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HRL43232.1"
                     /translation="MIDTKTAIEKITNGEFDNLFTDIYIDSSMIDYQKKRYVHAIEQY
                     ETIFCPDKVAIFSAPGRSEVCGNHTDHQHGMVLATSINLDTIAVSAKNNNDVVRFVSD
                     GYDMITLNINDLEVNDDEAGTTVSLIRGVLRGLKDHGYKIGGFNAYATSDVLVGAGLS
                     SSAAFEVVVGTIISGLYNDMKINSVEIAQISQYAENVFFKKPCGLMDQMACSVGGMVN
                     IDFKDPTKPIVKKVPTEFEKYDYSLCIVDTKGDHVDLTDDYAMIPSEMKKVANFLGED
                     YLRDCDSNEFYIRLDEIREAVGDRPVLRAIHFFNEEHNVKNAVSSLENGKFVEFLDAI
                     RKSGNSSFKYLQNVYTTRDIQHQNISVALALSESLLRNYGVCRVHGGGFAGTIQAFVK
                     NDFVSNYKEFINKIFGENSCHVLKVRKYGGIKVM"
     gene            4183..5175
                     /gene="galE"
                     /locus_tag="PLS64_05065"
     CDS             4183..5175
                     /gene="galE"
                     /locus_tag="PLS64_05065"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRL43233.1"
                     /translation="MKILVLGGAGYIGSHTVYRLIESGNEVVVFDNLETGHIEAVHPK
                     AKFYKGDLRNRSEIDSVFDKEKGIDAVIHFAANSLVGESMTNPLKYYDNNLNGTKVLL
                     QSMVAHGIDKIVFSSTAATYGEPERTPILETDPTNPTNCYGETKKSMERMFYWVEKAH
                     GLRYVSLRYFNACGAHISGKIGEAHNPETHLIPIILQAAQGTRDHISIFGTDYPTSDG
                     TCIRDYIHVTDLAQAHILAVEYLMKGGKSDIFNLGNGVGFSVREVIEKAKEVTQKDIK
                     VVEESRRSGDPAVLIASSDKAKTVLGWKPEYDDLGTIIKTAWKWHSTHPNGYSK"
     gene            5186..6697
                     /gene="galT"
                     /locus_tag="PLS64_05070"
     CDS             5186..6697
                     /gene="galT"
                     /locus_tag="PLS64_05070"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HRL43234.1"
                     /translation="MKNSVYGLILRLVSYGINTGLIEKEDIIYTQNRLLSLFHMDEPD
                     DSCTCLTADNEDTLEEILSGLLDYAYEKGIIPENTVTYRDLFDVKLMASLVKRPSEVI
                     REFWQNYSCSPKLATDSYYKFSCDTDYIRRYRIKKDLKWKTSTEYGDIDITVNLSKPE
                     KDPKAIAAAKLQKQSGYPKCLLCKENEGYAGRVNHPARDNHRIIPLTIDGDNWFMQYS
                     PYVYYNEHCIVFNGEHTPMVINDSTFRKLFDFISQFPHYIIGSNADLPIVGGSILTHE
                     HFQGGRYSFALNRAEVERKFIVNGFDDVECGIVKWPMSVVRLTGKNKDSIISLSSHIL
                     KSWRAYTDADVGIFAETDGEIHNTITPIAFTKDGKFVIDLVLRNNITSEEHPLGVFHP
                     HANLHHIKKENIGLIEVMGLAVLPSRLKDEMAILKDAILNHKDVSEIPKISKHSEWVN
                     EFVSQYDEINENNIDSIIQNEIGKVFLNVLLDAGVFKRTPEGQKAFDKFIKTL"
     gene            6714..7781
                     /locus_tag="PLS64_05075"
     CDS             6714..7781
                     /locus_tag="PLS64_05075"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRL43235.1"
                     /translation="MSKVRVVNFEPTSKGENTHLYTIENNSGASVTLCDLGASVVSIK
                     VPDKNGSIRDVVLGYEHIDGYEFDGTYFGATVGRCCGRIAYGKFTLNGEDYQLPVNNG
                     SNHLHGGFNSFSRKVWLAVVDDKVPNTVLFMLDSPDGDEGYPGNMKVFVRYTFDDENV
                     LTIKWSAVSDKDTICNLTNHSYFNLNGHKSGKVTENHKLKINANFFIPINSNLNPTGE
                     ITPVKNTSFDFTEPKLIGNGIDRKNEQIGFANGIDHMFVLNHSDEEFVLAAEAEGIDS
                     GITLKCYTSQKGVHVYTANYVDVLSNMGKDSATYGENPAFCLETQGFPDAVNHKNFPT
                     TILKANENYTQTTKYIFGINK"
     assembly_gap    8013..8022
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8306..9022
                     /locus_tag="PLS64_05080"
     CDS             8306..9022
                     /locus_tag="PLS64_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRL43236.1"
                     /translation="MLKIAVVDDEIVFVESLINKITAVCEKLNLEFKIDKYSNGFDIL
                     ENYSKYHLIFLDIEMPSIDGIATAKRINELKGSAEIPFIVYVTSHDELVFDALKSFPY
                     SFIRKSKIESDLYECINRINNSFEELHKTIVLHTERKDIPIVIGDIIYLEKIKNYVVY
                     HTKNQEYKVRSDMNTEFKNIAEYGFIRPHIGYAVNNKYVEYISADCILLQNDINIPLN
                     KKYRDDLKKKYFKWLGDINV"
BASE COUNT         2938 a         1481 c         1769 g         2835 t
ORIGIN      
        1 aaaactatag cttttgagta ggggtgcaaa atgaattttt agttattata attcattaac
       61 ggaattataa tcttccgact taatctatca tttgtggagt tataattata atttattata
      121 gcatacaatc gtttgataac gataataatt ataaactttt aggttttgca catgagttgc
      181 gaacttttat tggacaaggc agatagacac gcataggaga ataataatcg gtgcttttag
      241 gtttcatcag attcaaaata aatctaatgc cgtaaatgcc cattgcatat acaggtgcgt
      301 ttaccgtagt aagcggaggg tttgtatatc ccgaaagctc cgtgttatca aatcccataa
      361 cagaaatatc ctcaggaatt ttgtaaccgt tttcttgcag agctctcatt gcaccgattg
      421 caatatgatc acttgccgca aaaatagctg tggggagatt ccctttgctg atcatttctg
      481 acatcatttt ataaccggat gatgttgaaa atttgtcctg tataataaac ccatcatatt
      541 taatgtttag ttcatcgcaa atgtctgtga aagtgttgag tcgtttatca ggaaatatcg
      601 ttccgtcctc aagctgttca atgcctccga taaagccaac tgttctatgg cctaaatcat
      661 atagatattt aattgcatcg gacactgcct gacgaaagtc gagcgatacg gatgtgattt
      721 caatattctc aacggtcata tcaagaaaaa caattttatt attcaaagaa tgcagtaatt
      781 ttatttcttg tttactaaac tttccgaggc agattatgcc atcaacatca cagagtacat
      841 tctgatagtc attgtctgtt ttaaatgttc gtacaatact tatattattt tttgaacagt
      901 agtcctcgat accctgtctc atcaaaaagt aataattatc ttcaatttcc tgtttggaag
      961 agaaccattg tagaattcca atgcgacatg aaggtgctga tgtaaccctt tttttcttta
     1021 cataattaag ttctttagct gctgcaaata ttttttgctt tgtttcaagc gaggtgctga
     1081 gggtagtgtc gttattaagc actcttgaaa cagtagctac tgatacattt gcctttttgg
     1141 caacatctct gattgtagcc aattgaatca cctcactacc attatagtga aatattacta
     1201 aaaaatcaag tcgtgtttag tgaaaaacag agaaagcaaa aatgtttacc aaaaactatt
     1261 gacagtttac gaaacaaaac atataattta gttaacataa aaacaaaaga aacattatta
     1321 ttaaataata acgaaatctt ttgtgcaggt tgtcacggcg tttcatttaa aattaaaagg
     1381 aggaaaaatt atgaaacaga catcatcaca accaagactt gatggcaaaa tcccaggtag
     1441 aatcaaatac acctttgctt taggtgccct cggcaaagat atgatttatg gtatgattgc
     1501 cacattttca atgatttatt tcacagacat catcaaggtc aacccggtat ttatcggcgt
     1561 aatgttcttt gttgcaaagt tatgggatgc ctttaatgat ttgtttatgg gtatgattgt
     1621 tgataacaca cgcagcagat ggggtaaatt tattccatgg cttgtaatag gtacgctggt
     1681 taattcaatt gtatttgttg ttttattcac agacttccac ctcgatggcg ttggactttg
     1741 cgtatttgca acagcagctt acatactctg gggtatgact tacacaatta tggatattcc
     1801 ttattggtca attattccta acctcacttc aaatcctgag gagagagaaa aagtttcggt
     1861 acttccgaga atctttgcaa gtatcggtca gtcacttgtt attgcaggct ttggtgttca
     1921 gattattgag gctctcagcg gcggcacaac aaatcaggca ggttatcaca gatttgcaat
     1981 cataattgca attgtattta ttcttacaat cggtattaca gtaatcaatc ttccgaaaaa
     2041 gagagatgat tctgttccgg aaaagaaagt taagtttaaa gatattttct caatcatcgg
     2101 caagaatgat caacttcgtt gggcggtaac tcttatcttc ctttacaaca tcggcattca
     2161 gtgcattatg ggtgttgcta catattattt caaatatgtt tgcggcaacg caggaatgat
     2221 gtcatcattt atgatcagcg catcaatcgc agaggttgta ggtcttatcg tatttcctaa
     2281 ggttacaaaa tttctttcaa gaaaaacttc gttccttctt gcgtgtcttt tacctgcatt
     2341 tggtcttgta atgcttttgt tcgtaggtat ttttgctccg cacaatgttg tgctcacatc
     2401 aatctcaggt gttattgtaa aacttggtac aggtcttgaa cttggttgtg caacagtatt
     2461 ccttgcagat gttgttgatt acggtgaatt taagcttgga acaagaaatg aaggcgttgt
     2521 attctcactc cagacgctta tcgtaaaatt cacagcagct cttacagcac tcggtattgg
     2581 cgcggcactt ggaagcacac agtatattcc gggtcaggaa cagtcatttg caacagtagc
     2641 atcaatcagt acacttatgt gcattgttcc tgcggtttgt atgcttattg cttatgttgt
     2701 ttacagaaag aaatataaac tcaatgacgg tatgatgaag aaggttataa acacaatcag
     2761 tgcaagaaga gaaggcaaga ttgatatgac acaacagctt gatgaaagcg gtaacacaga
     2821 gccttctatg ttccttccta cagttgcgga aaaggcaggt aaataaaatg attgatacta
     2881 aaacagcaat tgaaaaaatt acaaacggcg aatttgataa tcttttcact gatatatata
     2941 tagattcatc aatgattgat tatcagaaaa aaagatatgt tcatgcaatt gagcagtatg
     3001 aaacaatttt ttgccctgat aaagtagcaa tattcagtgc tcccggcaga agtgaggttt
     3061 gcggtaatca tacagatcac cagcatggaa tggtgcttgc aacatccatt aatcttgata
     3121 ctattgctgt gtcggccaag aataacaatg atgttgtaag atttgtttct gacggttatg
     3181 atatgattac tctcaatata aatgacctcg aagttaatga tgatgaggca ggcacaacgg
     3241 tcagcctcat cagaggtgtt cttcgtggac ttaaggatca tggatacaaa attggtggat
     3301 tcaatgctta tgcaacaagt gatgtacttg ttggtgcagg actttcatcg tcagcagctt
     3361 ttgaggtagt tgtcggaact attatttccg gtttatataa tgatatgaaa ataaattcag
     3421 ttgagattgc tcagatttct cagtatgctg aaaatgtatt tttcaaaaag ccgtgcggac
     3481 ttatggatca aatggcttgt tcagtcggag gaatggttaa cattgacttt aaagatccga
     3541 caaagccaat tgttaagaaa gtacctaccg aatttgaaaa atatgattac agtctttgta
     3601 ttgttgacac aaagggtgat catgttgatc ttacagatga ttatgctatg attccctcgg
     3661 aaatgaaaaa ggttgcaaat tttctcggag aagattatct gagagattgt gattcaaacg
     3721 aattttatat tcgccttgac gaaattcgtg aggctgttgg cgacagaccg gtacttcgtg
     3781 caattcattt ctttaatgag gagcataacg ttaaaaatgc cgtttcgtca cttgaaaacg
     3841 gtaagtttgt tgaattcctt gacgcaatca gaaaatccgg taattcatcg tttaagtatc
     3901 ttcagaatgt atatacaaca agagatattc aacatcagaa catctcggta gcccttgcac
     3961 tcagcgaaag ccttcttcgc aattacggcg tgtgccgtgt tcacggcggt ggatttgcag
     4021 gtacaattca ggcgtttgtc aaaaatgatt ttgtaagtaa ttacaaagaa tttataaata
     4081 aaattttcgg agaaaactca tgccatgttc tcaaagtgag aaaatacggc ggaataaaag
     4141 taatgtaatt atgtaggttt tgtttttatt aaggaggaca aaatgaaaat tcttgtttta
     4201 ggcggtgccg gatatatcgg ttcgcataca gtttacagac ttattgaaag cggtaatgag
     4261 gttgttgtat tcgataacct tgaaacaggt catatcgagg cggttcatcc caaagcaaaa
     4321 ttttataaag gtgatctcag aaacagaagt gaaattgaca gcgtttttga taaagaaaaa
     4381 ggtattgatg ctgtaattca ttttgcagcc aattcacttg tcggtgaaag tatgaccaac
     4441 cctctcaaat attatgataa taacctcaac ggcacaaagg ttttgcttca gtcaatggtt
     4501 gctcacggaa ttgataagat tgtattttca tcaacagcgg caacatacgg tgagcctgag
     4561 agaactccta ttcttgaaac cgatccgaca aatccgacaa actgctacgg agaaacaaaa
     4621 aaatccatgg agagaatgtt ctattgggtt gaaaaagcac atggtttaag atatgtttct
     4681 ctcagatatt tcaatgcatg cggagcacat atcagcggaa aaatcggtga ggcacataat
     4741 cctgaaaccc accttattcc gattattctt caagcggcgc agggtacccg tgaccacata
     4801 agtatttttg gtactgatta tcccacttct gacggaactt gtattcgtga ttatatccat
     4861 gtcactgacc ttgcacaggc tcatattctt gcagttgaat atcttatgaa gggcggtaaa
     4921 agtgatatat tcaatcttgg caacggtgta ggtttttctg ttcgtgaggt aattgaaaaa
     4981 gcaaaagagg ttacacagaa agatattaag gttgttgaag aaagcagaag aagcggcgat
     5041 cctgctgtat tgattgcatc aagcgataag gcgaaaactg tacttggctg gaaacctgag
     5101 tatgatgatc tgggaacaat tattaaaaca gcgtggaaat ggcattcaac acatcccaac
     5161 ggctatagta agtgaggcgg tatttatgaa gaacagcgtt tacggcttaa tattaagact
     5221 tgtttcatac ggaatcaata cagggcttat tgaaaaagag gatataatct acacgcaaaa
     5281 taggctttta tcgttgttcc atatggatga acccgatgac agttgtactt gccttacagc
     5341 agataacgag gatactcttg aagaaatttt gtcgggactt cttgattatg catacgaaaa
     5401 aggaatcatt cccgaaaata cagtaacata tcgcgactta tttgatgtga aacttatggc
     5461 ttcacttgtt aagcgaccgt cagaagtaat aagagaattt tggcagaatt atagctgttc
     5521 tcctaaactt gcaaccgata gctactataa attcagctgc gataccgatt atataagaag
     5581 atatcgtatt aagaaagact taaagtggaa aacatcaacc gaatacggtg atattgatat
     5641 cacggtaaat ctttcaaagc ctgaaaaaga tccgaaagct attgcagcgg caaaacttca
     5701 aaaacagtcg ggctatccga aatgtttgct ttgtaaggaa aatgaaggat atgccggaag
     5761 agtaaatcat cctgcaagag ataatcacag aattattccg ctcaccattg acggagataa
     5821 ctggtttatg caatattctc cgtatgttta ttacaatgaa cattgtattg tatttaacgg
     5881 tgagcataca ccaatggtaa tcaatgattc tacattcaga aaactgtttg attttatttc
     5941 acagtttccg cattatataa tcggttcgaa tgctgatttg ccgattgtag gcggttcaat
     6001 acttacacat gaacattttc agggtggcag atacagcttt gcacttaata gggcagaggt
     6061 tgaaagaaag tttattgtta acggctttga tgatgtggag tgcggaattg taaagtggcc
     6121 tatgtcagtt gtaagattga caggtaaaaa caaggacagc attatttcat tatcttctca
     6181 tattctaaag agttggagag cgtacacgga cgcagatgtt ggcatttttg ccgaaacaga
     6241 cggtgaaatt cacaacacta tcactccgat tgcttttaca aaggatggaa aatttgttat
     6301 tgacctcgtt ctcagaaata atattacaag tgaagagcat cctctgggag tatttcatcc
     6361 tcatgcgaat ttgcatcata ttaaaaaaga aaatatcggc cttattgaag ttatgggact
     6421 tgcagtattg ccctcaaggc ttaaagatga aatggctatt ttaaaggatg ctattcttaa
     6481 tcacaaggat gtaagtgaaa ttccgaaaat cagcaaacat tccgagtggg taaatgaatt
     6541 tgtttcgcag tatgatgaaa tcaatgaaaa taatattgat tcaataattc aaaatgagat
     6601 aggaaaggtg tttcttaatg ttcttttgga tgcaggtgta ttcaaaagaa ctcctgaagg
     6661 acaaaaggct tttgataaat ttataaagac gctgtagtaa aggaagtgct aaaatgtcaa
     6721 aggtaagggt agtgaatttt gaacctacat ccaaaggtga aaacactcat ctttatacaa
     6781 tagaaaataa cagcggagca agcgtgacat tatgtgattt aggcgcaagt gtcgtttcga
     6841 taaaagttcc cgacaaaaac ggtagtatta gagatgtagt tctcggctat gagcacattg
     6901 acggttatga atttgacgga acatacttcg gtgctactgt cggaagatgt tgcggaagaa
     6961 ttgcatacgg caagtttact cttaacggag aagattatca gctccctgta aacaatggga
     7021 gtaaccatct acacggcgga tttaacagct ttagccgcaa agtgtggctt gcggtagttg
     7081 atgataaagt tccaaataca gttctattta tgcttgatag tcccgacggt gatgagggct
     7141 atcccggaaa tatgaaggtc tttgtacgct acacctttga tgatgaaaat gtacttacga
     7201 taaaatggtc ggcagtttcc gacaaagaca caatatgtaa tctgaccaat cattcatatt
     7261 tcaatcttaa cggacataaa agcggtaaag ttacagaaaa tcacaagctg aaaataaatg
     7321 caaatttctt cataccgata aattcaaacc tcaatcccac aggagagata actcccgtta
     7381 agaatacatc gtttgatttt accgaaccca agttaattgg aaatggtatt gacagaaaaa
     7441 acgagcagat tggttttgca aacggtattg atcatatgtt tgtgctgaat cattctgatg
     7501 aagaatttgt gcttgcggcc gaagctgagg gcattgatag cggaatcaca ttaaaatgct
     7561 atacttctca aaaaggtgtt catgtatata cagcaaatta tgtcgatgtt ttgtccaata
     7621 tgggaaagga ttcagcaaca tacggagaaa atccagcgtt ttgtcttgaa acccaaggct
     7681 ttcccgatgc tgtaaatcac aagaattttc ctacgacaat attaaaagca aacgaaaact
     7741 atacacaaac tacaaaatat atttttggta taaacaaata aaataagcta atttctaact
     7801 ccttttaaaa ataaaatctc tctaaaatca aggaccacct atttttcttg ggtggtcctt
     7861 gatttttaat ctatgaggat gtttcatgtt ttacagtatt acaccgtctt aaacatattc
     7921 tgttttagcg gttatgtgca attctgcctg aaaatcaccc tgttctgttt gcaccgtaaa
     7981 accggaacat tcctcttgca cggctgtttt tannnnnnnn nnacaaactg catttttcat
     8041 tgcccgtgta tttcttagcg ctgtttttgc ttttatatta aaatacgctt aggatagatt
     8101 tctttaccct ccgcatcaaa ttcaaaattt tcattacccc aacttttaaa aaaataatca
     8161 cttttcccta gcaaatgtac agagataacg gcggtaataa cttgctgact tataaaattg
     8221 atagtaactt cttaaatgat cgactgttta agagtgtatt gaaaaacatt ttaatatttg
     8281 ttagaattaa aatggtggtg aggatatgtt aaagattgcg gtagtagatg atgaaattgt
     8341 atttgttgaa agtctgatta acaaaataac agcggtatgt gaaaagctta atttggaatt
     8401 taaaattgac aaatactcaa acggatttga tatacttgaa aattacagca agtatcatct
     8461 gatttttctt gatattgaaa tgccgtcaat tgacggtatt gctacagcca agcggatcaa
     8521 cgaattgaaa ggttcagctg aaataccttt tattgtttat gtcacaagtc acgatgagct
     8581 tgtgtttgat gctttaaaaa gttttcctta ttcatttata agaaaaagta aaattgaatc
     8641 tgatttgtac gaatgtatta accgtataaa taattctttt gaagaattac ataaaactat
     8701 agtattgcat accgaaagaa aagatattcc gattgttatt ggtgatatta tttatcttga
     8761 aaaaattaaa aattatgttg tgtatcatac taaaaatcaa gaatataagg tcagaagtga
     8821 tatgaataca gaatttaaaa atatcgcaga atatggcttt ataagaccgc atattggtta
     8881 tgctgtaaac aataagtatg ttgaatatat ttctgcggat tgtattttat tgcaaaatga
     8941 tattaacatt cctcttaata aaaaatacag agatgattta aagaaaaaat atttcaaatg
     9001 gttgggggat ataaatgttt aattatggtt ttg
//
LOCUS       DAOMVE010000067         8855 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2022_length_8855_cov_3.044545, whole genome shotgun sequence.
ACCESSION   DAOMVE010000067 DAOMVE010000000
VERSION     DAOMVE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8855
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2022_length_8855_cov_3.044545"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PLS64_05085"
     CDS             complement(<1..1062)
                     /locus_tag="PLS64_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43237.1"
                     /translation="MKRVLSLAIVLMLAFSCVFTAFAQTDDTASPDEAKKVTELKITK
                     LPDKLTYTAEDVIEPDIDLSKIADENATEESLIKYFSQFNLELKLDLTGMEIEAVYSD
                     GTTEKVDAKDCKAELADPFNYGEVIKALIEYEKNMPEFTEDMTEDEFYELLEKALKVL
                     VDMIYREYTVNVSYQDAQTSYKITLENSDDPFNPDLPDDNDRYEVVSVKAPEKVNYTK
                     ADTDFFYEADMPYDGSPESKEYYDETYFYPEFKGLEVTVKDKITGKTYTKTFDGTEED
                     MVYISPVYVRPDDTMTNLEVDVMGDVNEWLNLPEDEEDSTEVTGTFKVNYYANGVPSK
                     DISSTKDTATKDTVTKSATG"
     gene            1389..1586
                     /locus_tag="PLS64_05090"
     CDS             1389..1586
                     /locus_tag="PLS64_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin thioredoxin reductase catalytic beta
                     chain"
                     /protein_id="HRL43238.1"
                     /translation="MKILLNPDKEIVKEVRKQLIRTGGYCPYMLIRSPATLCMCEDFR
                     AKIADPEFEGYCHCLMYYKQK"
     gene            complement(1669..>2996)
                     /locus_tag="PLS64_05095"
     CDS             complement(1669..>2996)
                     /locus_tag="PLS64_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL43239.1"
                     /translation="KNLLSDNEYKEICGYLNTPTDNDGNYYILICKKFYESEKYGYVP
                     CQLEVVKTNKVNDWYVQDEVVKTYSFDLSKYDYLTIEELEATPFENDEMHRNIIDKDF
                     FLGKSKPKYSQKDVEKILSDILSDNSLFTDIYDETGAVPDGIFKYYVLMGDSLYYTEN
                     HMINNSPNEYKEVTRVFHITSLKEINLLDDCLWKFITVFAVAFTIMAVVITVEIFSWK
                     DFKRRTAQEETRLEMTNAIAHNLKTPLFVIGGFAENLKNEEDTEKKLHNIEIIQQQTE
                     EMDILIHRMLDLSRFDSPALKLNCEVFDFKPFIEKILENYYVHTEHEIIFIYNSKAKI
                     KADPAMLKTVLENLIDNAEKYSDYGSDITINVSDDVLSISNQCSSFDRENLSKLWQPY
                     FRAAKGDNKSGSGIGLSIVKSALEMHGFKYKAEFENGIITFKFRFTSLD"
     assembly_gap    2997..3096
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3180..3860)
                     /locus_tag="PLS64_05100"
     CDS             complement(3180..3860)
                     /locus_tag="PLS64_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL43240.1"
                     /translation="MYNILLVEDDKNIREMLVEYFSKRDKEKFNITLAENGLKGLDKA
                     YENSYDLLLLDVMLPEMDGFTLCKEIRRYSDVPIMFITARTSEADMLNGYALGCDDYI
                     VKPIALPVFYEKVKALIKRSKGLVRHNLLTVGNVSLNPNNGVVVSDGEEVTLTAKEYA
                     ILKILLENKNSVVSREKIITAVWHYDTMIDERVLDTHIKNIRKALGENASVLKTVIRR
                     GYKAEDKK"
     gene            complement(3994..4392)
                     /locus_tag="PLS64_05105"
     CDS             complement(3994..4392)
                     /locus_tag="PLS64_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRL43241.1"
                     /translation="MNVIMLLKHKDSLKYIYEDNTLRQGLEKMRAHSYTAIPVITRDG
                     KYVGTVSEGDFLWHIIDKKTSGDIEEQEEFRIRDILRPDFNPAVKISVGMDELLARAM
                     NQNFIPVTDDLGTFIGIVTRQDIIRYFVNK"
     gene            complement(4492..4950)
                     /locus_tag="PLS64_05110"
     CDS             complement(4492..4950)
                     /locus_tag="PLS64_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43242.1"
                     /translation="MDFFSTNLFNWYGLAFAIALLVPDIVYIKSNRIDRTIFDNRAML
                     YIERIGKYCSIFLMGINIGVLEGGFTKPIMETFWIISTCVLTVVALILWFLCFNRFSK
                     LWAYLLTAVTAVIFMMSGLLQVKTLLLTAGVVYLIGQLYVTNKYVKSKMQ"
     gene            complement(4963..5496)
                     /locus_tag="PLS64_05115"
     CDS             complement(4963..5496)
                     /locus_tag="PLS64_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43243.1"
                     /translation="MDTMSVNGKDFTVIKLLGKGKGGYSYLVSDGEHNYVLKQIHHEP
                     CSYYTFGNKIQSELNDYERLKNAGLRLPKLIDCDVENERILKDYIDGKTAYELVLQNE
                     MKPEYIEQIREMCRLLNPKGINIDYFPTNFILNNGLLWYIDYECNDYMEEWNFENWGI
                     KYWSKTKEFCEYHENNK"
     gene            5794..6942
                     /locus_tag="PLS64_05120"
     CDS             5794..6942
                     /locus_tag="PLS64_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRL43244.1"
                     /translation="MEKKKFRISGKLIFNLAVVAISIYLIIYFFVSEDGLIDLLKKPD
                     GCNMLIILAALLIYDLNILVDAVVTLIYVRSKYENFSFIEALKVAFVGVFFSAITPSS
                     TGGQPMQLYLMSKKNISVGFGSACMTQKFIVYQIVMTAVSVIAVITRFNYFSDAFTNI
                     WSTLFIIFGFTVQLAMTALFLLVSFSRKVTNKLINLIYKIMKKFKFIKNPEKKVERLH
                     KEVDMFHDTNKQLFSSVKRLVVIYALVFIQVILILSIPYLIYVSFGMAPIARAAGQPT
                     LSFYDSICIQSFVLFTANLIPLPGASGGAELAFTMYFGSFFKIGAISKIKPAILMWRF
                     ITYYGAMLISAPFSYFTKGKRRDDEIKQIEEELESEMDNKKTEEKVTQ"
     gene            complement(7092..8765)
                     /gene="hcp"
                     /locus_tag="PLS64_05125"
                     /gene_synonym="priS"
     CDS             complement(7092..8765)
                     /gene="hcp"
                     /locus_tag="PLS64_05125"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HRL43245.1"
                     /translation="MSNNMDLGYEMFCYQCEQTANGKGCTRLGVCGKTPEIANLQDLL
                     IFQLKGISCYGKVLIEKGQHIDKDIVRFVENCLFTTLTNVNFDADVHVSLLRESQQIK
                     EKLREVVGEIKNHTLHATYNLPETKSEMLKDAPLAGIMYEKSLDPDIRSLRQTIVYGL
                     KGISAYGHQARELGYFSDQVDDFYITALEATTDDSLTVEELIRMTMRTGENALEVMKK
                     LDEANTETYGNPSPHKVDVHIKKGPFIIVSGHDLKDLEMLLEQSKGKGINVYTHGEML
                     PCHGYDGLKKYPHLIGNFGGAWQDQQKQFDNIPGCILMTTNCLMRPRDSYKDRIYSTN
                     VVGWEGVKHIGKKENGDKDFSEIIQQAIELGGFKEDVEPHEILVGFGHHATLSYADKI
                     VEAVKSGKVRHFFLIGGCDGARPGRNYYTEFAENVPKDCIIMTLACGKYRFNKLEFGD
                     IDGLPRLLDIGQCNDVYSAICIAVALADAFDTDVNGLPLSLIVSWYEQKAVADLLALL
                     SLGIKNIYLGPTLPAFLSPNVLQYLSETFGLRPISNAEDDLKTCLKQNV"
BASE COUNT         2503 a         1819 c         1572 g         2861 t
ORIGIN      
        1 gcctgttgca gattttgtta ctgtatcctt tgttgcagta tcttttgttg aagaaatatc
       61 ttttgaagga actccgtttg catagtagtt aaccttgaat gtacctgtaa cctctgttga
      121 atcctcttca tcttcaggaa gattgagcca ttcgtttaca tcacccataa catcaacttc
      181 aagatttgtc attgtgtcat cagggcgaac ataaacagga cttatatata ccatatcttc
      241 ctctgtgccg tcaaaagtct ttgtgtaagt tttgcctgta attttatcct ttacagtaac
      301 ttcaagacct ttaaattcgg gatagaaata tgtttcatcg tagtattcct tgctttcggg
      361 acttccgtca tacggcatat cagcctcata gaagaaatct gtatcagcct ttgtgtagtt
      421 caccttttcg ggtgctttta cagaaaccac ttcgtaacgg tcattgtcat cgggtaaatc
      481 gggattaaac ggatcgtcac tattttctaa tgtaatctta tagcttgtct gtgcatcctg
      541 atatgataca tttacagtat actctctgta aatcatatca actaaaactt taagagcttt
      601 ttcaaggagt tcataaaact catcttcggt catatcttct gtaaactcag gcatattttt
      661 ctcatattca atcaatgcct taattacttc gccgtaatta aacggatcag caagctcagc
      721 tttgcagtcc ttagcatcaa ccttttcggt tgtaccgtca ctgtaaacag cttcaatttc
      781 catgcctgta aggtcaagtt tgagttcaag attgaactga ctgaaatact tgattaggct
      841 ttcttctgtt gcattttcgt ctgcgatttt tgataaatca atatcgggtt caatcacatc
      901 ctctgctgtg tatgtgagct tgtcggggag ctttgtaatt ttaagctctg ttaccttttt
      961 tgcctcatcg ggtgacgctg tgtcgtctgt ctgtgcaaac gctgtgaaca cgcatgaaaa
     1021 tgcaagcatc aaaacgattg caagagataa tactcttttc ataaattttt cctcctgaaa
     1081 actaaaaatc attgtatgat aaatgtatat acaatcacaa cgctattgta gcacactatt
     1141 ttgcaaaaat caactgtttc aacaaataat tgtcgcaaaa attcttcact tgctgttttt
     1201 tatgaatttt ttatatagta tattattgca tttgagattt aattataatt gttcaaataa
     1261 atttgtattt attgcaaatt ttctattgta aaattctgtc attgctgata taattgtagg
     1321 cggaatcaaa agattaccga atacccgaaa atcggcataa aatctaccca acaaatatgg
     1381 tgattaaatt gaaaattctg cttaatccag acaaagaaat tgtaaaagaa gttcgcaaac
     1441 agcttatacg cacgggcggt tattgtccgt atatgttaat cagaagccct gcaacactgt
     1501 gtatgtgtga ggatttcagg gcaaaaattg ccgatcccga atttgaggga tactgccatt
     1561 gcctgatgta ttataaacaa aaatagaatt ttatacataa caaaataaca aaaatcggaa
     1621 cagccgtgat tgggtttacg gctgttccga ttttttaggc agaaaaactc aatcgagtga
     1681 agtaaatctg aacttaaatg ttataatccc gttttcaaat tcggctttat atttgaatcc
     1741 atgcatttca agggcggatt ttactattga aagtcctatt ccgctgccac tcttattatc
     1801 tccctttgcg gctctgaaat aaggctgcca caattttgag agattttccc tgtcaaatga
     1861 cgagcattga ttgctgattg ataatacatc atccgaaaca ttaattgtga tatccgaacc
     1921 gtaatcggaa tatttttcgg cattgtcaat aagattttca agcaccgttt taagcatggc
     1981 agggtcagcc tttatttttg cctttgagtt gtaaatgaaa ataatttcgt gttctgtgtg
     2041 aacataataa ttttcaagaa ttttctcaat aaacggttta aagtcaaaaa cttcgcaatt
     2101 cagctttaat gcgggcgaat caaatcgtga caaatcgagc attctgtgaa taagaatgtc
     2161 catttcctcg gtctgttgct gaatgatttc tatgttgtga agtttctttt cggtatcttc
     2221 ctcattttta agattttccg caaaaccgcc tatgacaaac aagggggttt tgagattgtg
     2281 cgcaattgca tttgtcattt caagacgggt ttcctcctgt gcggttcgcc ttttaaaatc
     2341 tttccacgag aaaatttcaa ccgtaatcac caccgccatt atcgtaaacg caacagcaaa
     2401 taccgttata aacttccata ggcaatcatc aagcagattt atttctttta aagaagtaat
     2461 atgaaatact cttgtaacct ctttatattc attcggcgag ttgtttatca tatgattttc
     2521 ggtataataa agggaatctc ccattaatac ataatattta aaaatcccgt caggaactgc
     2581 cccagtctca tcatagatgt ctgtaaacag actgttgtca ctcaggatgt cactcaggat
     2641 tttttcaaca tctttttggg agtatttcgg tttgcttttt ccaaggaaaa agtccttatc
     2701 aattatatta cggtgcattt cgtcattttc aaacggagta gcctctaatt cttctatggt
     2761 cagataatca tatttgctaa ggtcaaagct atatgtttta acaacttcat cctgaacata
     2821 ccaatcattc actttgttgg tctttacaac ctcaagctga caagggacat agccgtattt
     2881 ttcgctttca taaaactttt tgcaaatcaa gatatagtaa tttccgtcat tatcagtcgg
     2941 agtgttgaga tatccgcaaa tttctttata ctcattatca ctgagcaaat ttttaannnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaacc gttcccagca gacacacaat
     3121 tattgttacg gaaattataa tgtaatgaaa cttttttcgc cgtttgatat atcttgattt
     3181 cattttttat cctccgcctt ataccctcgc cgtattaccg ttttcaaaac agacgcattt
     3241 tcgcccaatg cttttcggat atttttgata tgcgtgtcaa gcactctttc atcaatcatt
     3301 gtgtcatagt gccaaacggc tgtgatgatt ttttcacggg acacaacgct gtttttattc
     3361 tctaacagaa ttttaagaat agcatattcc tttgcggtaa gggttacttc ttcaccgtcg
     3421 gacacaacaa caccgttgtt cggattgagc gaaacattcc ccaccgtaag caaattgtgc
     3481 cgcacaagtc cttttgaacg cttgatgagg gctttcacct tttcataaaa tacgggcaga
     3541 gcaatcggct ttacaatgta atcgtcacag ccgagagcgt agccgttgag catatccgcc
     3601 tcgcttgttc ttgccgtgat gaacataatc ggcacatcgc tgtatctgcg gatttctttg
     3661 caaagcgtaa aaccgtccat ttcggggagc attacatcaa ggagcaaaag gtcatagctg
     3721 ttctcatatg ccttatcaag tccctttaag ccgttttccg caagggtgat attaaatttt
     3781 tctttatctc tttttgaaaa atattcgaca agcatttcac gaatattttt atcgtcctca
     3841 accaaaagaa tgttgtacat tttcctcacc gtactttgat tgtaatacat taatctgaag
     3901 ttgatatgta gatttctgta tatttaaaat tcagtttctg tcataactaa aacggcggat
     3961 gcaattttca gcatccgcct tgttttatgt atcttattta ttgacaaaat atctgatgat
     4021 atcctgacgg gttacgatac cgataaaagt accgaggtca tcggttacgg ggataaagtt
     4081 ctgattcatt gcccgtgcaa gaagctcgtc catacctacc gaaattttaa ctgccggatt
     4141 aaagtcggga cggagtatgt cacggatacg aaattcttcc tgttcttcaa tatcgcccga
     4201 agtctttttg tcaattatat gccacaaaaa gtcaccctcg ctcaccgtgc cgacatattt
     4261 tccgtcacgg gttataacag ggatagcggt gtagctgtga gcacgcattt tttcaagtcc
     4321 ctgtcttagg gtattgtcct cataaatata ttttaaagaa tctttatgct ttaaaagcat
     4381 aataacattc atcttttcac ctccagtcaa aaaacaaata tttgtcagcc aaaggtcatt
     4441 atagtaccaa acaaacaata ttgcaatatc tttttacaat attttacaca attattgcat
     4501 ttttgatttt acatacttat ttgtaacata aagctgtccg ataaggtaca ccacgcctgc
     4561 cgtgagcaaa agcgttttaa cctgcaaaag tccgctcatc ataaaaatta ctgcggttac
     4621 ggctgtaaga agatatgccc ataattttga aaatctgtta aagcataaaa accacaaaat
     4681 aagtgcaacg accgtcagaa cacaggttga gattatccaa aatgtttcca taatcggctt
     4741 tgtaaaaccg ccctcaagca cgccgatgtt gattcccata aggaaaatac tgcaatattt
     4801 tccgattctt tctatgtaaa gcatagccct gttatcaaaa attgttctgt ctattctgtt
     4861 gctttttata tagacgatgt cgggtacaag caaagcaatt gcaaaagcaa gtccgtacca
     4921 gttaaaaaga tttgtactga aaaagtccat aagttcactc cgttatttat tgttttcgtg
     4981 gtattcacaa aattccttcg ttttgctcca atactttatt ccccagtttt caaaattcca
     5041 ctcctccata taatcgttac attcgtagtc gatgtaccac aaaagtccgt tgtttaaaat
     5101 gaaatttgtc gggaaataat ctatgttaat tccttttgga ttcaacagtc tgcacatttc
     5161 acgaatctgc tcaatatatt cgggtttcat ctcattctgc aaaacaagct catatgcggt
     5221 tttgccgtca atgtaatctt taagaatcct ctcgttttct acatcgcagt caataagctt
     5281 cggcagtctt agcccggcat ttttcagccg ttcgtaatcg ttaagctcac tttgaatttt
     5341 gttaccgaag gtatagtatg aacacggctc gtggtgaatc tgctttaaaa cataattgtg
     5401 ttctccgtct gacacaaggt aggaataacc gcccttgcct tttccgagaa gcttaatgac
     5461 ggtaaaatct tttccgttca cactcatagt atccatacga acacctccgc acatattacc
     5521 gtattatttt atcacagcta aagtttgggt gcaagtttta aattctttat gtgtaatgaa
     5581 caaatgctaa aataagtaca ttcggtaaaa taaaaaagcg tggatttata aatttatttt
     5641 cccatgcggc aatttattac aaatttgttg ttgattttct tattaaaaaa aagtatgata
     5701 tagaatatat tattctgccc aaatgccaca gttgtgacat ttgatgccca ctacaattga
     5761 ataacaactt ttaacacaca ggagaagatt tatttggaaa agaaaaaatt cagaatcagc
     5821 ggaaagctga tatttaatct cgctgttgtg gcaatctcga tttatctgat catctacttc
     5881 tttgtttcgg aggacggtct gatcgacttg ctcaaaaagc ctgacggctg caatatgctg
     5941 attatccttg cggctttgct gatttatgat ttgaacatcc ttgttgacgc tgttgtcacg
     6001 ctgatatatg tgcggtcaaa gtatgagaat ttcagcttta ttgaggcttt gaaggtcgca
     6061 tttgtcggag tattcttcag tgcgattaca ccgtcaagta caggcggaca gcctatgcag
     6121 ctttacctta tgtcaaaaaa gaacataagc gtaggcttcg gttcggcttg tatgacgcaa
     6181 aagttcattg tgtatcagat agtaatgacc gctgtcagcg ttattgctgt tatcacaagg
     6241 ttcaactatt tcagcgatgc ttttacaaac atctggtcaa cacttttcat tattttcgga
     6301 tttaccgttc agcttgcaat gacggctttg ttcctgcttg tttcattcag ccgtaaggtt
     6361 acaaacaagc tcatcaatct tatttataag attatgaaaa agttcaagtt cattaaaaat
     6421 cccgaaaaaa aggttgaaag acttcacaaa gaagttgata tgtttcacga cacaaacaaa
     6481 cagctttttt caagcgtaaa acggcttgtt gtaatttatg cacttgtatt cattcaggta
     6541 attttaattc tttcaatccc ctacctcatc tatgtttcat ttggtatggc tccgattgca
     6601 agagcggcag gtcagccgac actttcattc tatgattcaa tctgtattca gtcattcgtg
     6661 ctgttcacgg caaaccttat cccgctcccc ggtgcatcgg gcggtgcgga gcttgcattc
     6721 acaatgtact tcggttcatt cttcaaaatc ggtgcaataa gcaaaatcaa gccggcaatc
     6781 cttatgtgga gattcattac atactacgga gcaatgctga tttctgcccc gttctcctac
     6841 tttacaaagg gtaaaaggcg tgacgatgaa ataaaacaaa ttgaagaaga acttgaatcg
     6901 gaaatggata ataaaaaaac tgaagaaaaa gtaactcagt agtattttca taaatatctc
     6961 tttcaaaaac ggagcagaca caattcagtc tgctccgttt ttgttatatg acaaaccgtt
     7021 agccattttt tacggtcaat tgttggaaac aatttgtagc ggcgaaccgt gttcgccgct
     7081 acagtatatc atcaaacatt ctgtttaagg caggttttca ggtcatcttc tgcgttgctg
     7141 attggacgca agccgaatgt ttctgacaaa tactgcaaca cattcgggct taaaaatgca
     7201 gggagtgtcg ggccgaggta gatattcttt attccgaggc ttaaaagtgc cagcaaatcg
     7261 gcaaccgcct tttgctcata ccacgaaaca atcagcgaca acggcaagcc gtttacatct
     7321 gtatcaaacg catcggcaag tgctacggca atacagattg cggagtaaac atcgttgcac
     7381 tgaccgatgt cgagtaatct cggcaagccg tcgatgtcac cgaattcgag cttgttaaag
     7441 cggtattttc cgcaagcgag cgtcataatt atgcagtctt tcggcacatt ttcggcaaac
     7501 tccgtgtagt aatttctgcc cggtcttgca ccgtcacaac caccaataag gaaaaagtgt
     7561 ctgacttttc cgcttttgac ggcctcgaca attttatcgg catatgaaag cgtagcgtga
     7621 tgaccgaatc ccacaagaat ttcgtgcggt tcaacatctt ccttaaaacc tccgagttca
     7681 attgcctgct gaataatttc gctgaaatct ttgtcgccgt tttccttttt gccgatgtgc
     7741 ttaacaccct cccagccgac aacatttgta ctgtaaattc tgtctttgta cgaatctctc
     7801 ggccgcataa ggcagtttgt cgtcataaga atacaaccgg ggatattgtc aaactgcttt
     7861 tgctgatctt gccatgcacc gccgaaattg ccgataagat gcggatattt ttttaaaccg
     7921 tcatagccgt gacacggcag catttcgccg tgagtataaa catttatgcc cttgcccttt
     7981 gactgttcaa ggagcatttc aaggtctttc aaatcgtgac ccgacacaat aataaacgga
     8041 ccttttttga tgtgaacatc aaccttgtgc ggtgacggat tgccgtaggt ttcagtgttt
     8101 gcttcatcaa gttttttcat aacttcaaga gcattttcac ccgttctcat cgtcattctg
     8161 ataagctcct caaccgtgag cgaatcatcc gttgtcgcct caagtgcagt gatgtaaaaa
     8221 tcgtcaacct gatcgctgaa atagccgagt tcacgagcct gatgaccgta ggcgcttatg
     8281 cctttgagac cgtaaacaat tgtctgtctt aaagagcgga tgtcggggtc aagcgacttt
     8341 tcgtacataa ttcccgcaag cggtgcgtct ttgagcattt ccgactttgt ttcgggaaga
     8401 ttgtaggttg cgtgcagagt atgatttttg atttcgccca caacttcacg aagtttttcc
     8461 ttgatttgct gagattcacg caaaagcgac acatgcacat cggcatcaaa gtttacattc
     8521 gtcagcgttg taaaaaggca gttttccaca aagcgaacaa tgtccttgtc aatgtgctga
     8581 cctttttcaa taagcacctt gccgtaacag cttatgccct tcagctgaaa gataagtaaa
     8641 tcctgaagat ttgcaatttc gggagttttt ccgcaaacac cgagccgtgt gcaacccttt
     8701 ccgttcgcag tctgctcaca ctgatagcaa aacatctcat aacccaagtc catattatta
     8761 ctcataaaaa ttacctccga aacattctgc aaatattatc tgcaaatttt cggaggttta
     8821 ttcttctatc tttttccctg acctattttt tcagc
//
LOCUS       DAOMVE010000068         8838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2027_length_8838_cov_2.996243, whole genome shotgun sequence.
ACCESSION   DAOMVE010000068 DAOMVE010000000
VERSION     DAOMVE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8838
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2027_length_8838_cov_2.996243"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..894)
                     /locus_tag="PLS64_05130"
     CDS             complement(58..894)
                     /locus_tag="PLS64_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRL43246.1"
                     /translation="MFDMIFEMFSYNFIVRAFIVGTLVSLCAALLGVTLVLKRYSMIG
                     DGLSHVGFGALAVAAALNLAPLEVAVPVIIIAAFLLLRLSENSKINGDAAIAIISSTA
                     LAIGVVFVSVSGVNTDLNSYLFGSILATTTADAIMCGILALVVIVIFILFYNKIFAVT
                     FDETFSRATGLKTGVYNTVIALLTALTIVIGMRIMGTLLISALIIFPALSSMRVCKKF
                     KSVILCSGVLSLCCFFVGMCVSYSCDTPTGASVVIVNAAVFLIFRLVEFINSKIKKNN
                     SV"
     gene            complement(887..1633)
                     /locus_tag="PLS64_05135"
     CDS             complement(887..1633)
                     /locus_tag="PLS64_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43247.1"
                     /translation="MSLIKIENASLGYEGKTVVNDISVDIKEKDYICIVGENGSGKTT
                     LMKALLGLLKPLSGSITYSDGLKQNEIGYLPQSSDIQRDFPASVQEVVLSGCLNKKHT
                     FFYSKEQKKTAAENMKLTGIYDLRKKCFRELSGGQRQRVMLARALCATHKLIILDEPV
                     TGLDPMAMADMYALIKKLNESGITIIMVSHDVNSAVSNASKILHLAKNWYFYGTAHQY
                     LHSDAGKRFILSDCPCDECQHRVHGGAKNV"
     gene            complement(1633..2619)
                     /locus_tag="PLS64_05140"
     CDS             complement(1633..2619)
                     /locus_tag="PLS64_05140"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRL43248.1"
                     /translation="MKKLVSIFLSAVIICGLLLISACDRTEDVRKSDGKISVVTTIFP
                     YYDFVRQLAGDKVDIRLLLSPGSDPHSYEPKPSDITAIENCDIFICNGGESDEWVDGV
                     LSSIENKDVKVMKMMEYVPLRHEQSMDHDHEHDSHEDMDDDDEGHDHEEGEEYDEHVW
                     TSIRNAERMSASIADELMSVDSKNSKYYNDRKTNYISQLDSLDKKFTEVANNKKRDTL
                     VFGDRFPFLYFVSDYNLGYECAFPGCSHETEPSTAVVSHLIDFTREHDIPVVFYLELS
                     SGKIARIISEDSSAKTMQFSSCHNVTKNDFENGATYISVMEQNLEALKEALY"
     gene            complement(2738..3763)
                     /gene="secF"
                     /locus_tag="PLS64_05145"
     CDS             complement(2738..3763)
                     /gene="secF"
                     /locus_tag="PLS64_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRL43249.1"
                     /translation="MTDKTQTKAVSNAPLTDEEFAEKYNRGHKIIDFVGHKKIFFGIS
                     LAIIIIGIVCNFIFGTQLDIQFSGGATLKFSYSGEINQSELTDFIQVETTEDRITTTY
                     AQDLMGDSGTTVTVQFSGNDSIKTDIQKNLESDLQKKYPDNNFKCLESNSVDASMGFS
                     FLLKCLTAVAIAAVLMVLYVTIRFKKIGGLSAGVMALVALFHDVAMIYFMYVIFQMPI
                     DASFIAVVLMILGYSLNDTIVIYDRVREEKKLVGSKTDVGKIFNLSCTKCIKRTIMTS
                     VTTLSAILVVYVVACAFNITSVQGFALPMIIGIISGCYSSICIAGPLWVMWQRHKKAK
                     KLAAKAA"
     gene            complement(3756..5177)
                     /gene="secD"
                     /locus_tag="PLS64_05150"
     CDS             complement(3756..5177)
                     /gene="secD"
                     /locus_tag="PLS64_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRL43250.1"
                     /translation="MKRVPKPVFFVVALLILAFSFTAIFGISYYTGDNKTTVLKGISD
                     IRWGIDIRGGVEATFKPAGDVDATDEQLESAKSIIELRMVSQGITDYELYADAKSDRI
                     IVRFPWKEDSDQNAIEAIEEISSTAQLTFRPGDGHETTDVDSNGGYVYKTPSGDTAKT
                     ILMDGSYVESATAGMTTDKTTGKNSYVVQLKLKSSGDKNGKERFADITKEYLNQQVSI
                     WMDDIMLSAPKVEAEITDGNAVISGDFTAKEAQDLASKINAGALPFQLETSNYGTISA
                     TLGENSLVAMAYAGVIAFIIIAIIMILFYRLPGFVAVISLLGQMGLAIAAVSGYFPVF
                     SSFTMTLPGIAGLILSIGMGVDCNVITAERIKEELKAGRTLDGALERGTKNSLSAIVD
                     GNMTVIIVSIILMLVFGPANILSIIFGESTTGTIYSFGYTLLVGVIANFIMGVFCTRI
                     MLRSVAGFKFLRKKWLFGGAKND"
     gene            complement(5428..6708)
                     /locus_tag="PLS64_05155"
     CDS             complement(5428..6708)
                     /locus_tag="PLS64_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL43251.1"
                     /translation="MGYLDLSKQELEKEYGNLVKRFEEIKGMGLNLNMARGKPSKEQL
                     DFSLPMLDTINSKSDFIGEDKMDCRNYGLLNGITECRKLFADILGVDAENVIVGGSSS
                     LNMMFDTISCFMTAPAVEGCKPWYEVKNRKFLCPVPGYDRHFGITQYYGFELIPIPMD
                     DNGPDMDMVERLVSEDDSIKGIWCVPKYSNPAGITYSDEVVKRFAALKPAAKDFRIMW
                     DNAYCIHEISDTPDVLLNIMDECKKTGNEDLPILFCSTSKITFPGAGVAAMAASENNL
                     KVLREKYNYEIISYDKLNMLRHVRFFKDYDGVLKHMEKHKSVLKPKFEIVLDTLDKEL
                     APADIASWKKPHGGYFVSVDVFEGTAKRTVALCKEAGVVLTGAGATYPLGIDPKDSNI
                     RIAPTFPPNHELEVAMDVFCICARLAACEKILNK"
     gene            complement(6934..7467)
                     /locus_tag="PLS64_05160"
                     /pseudo
     CDS             complement(6934..7467)
                     /locus_tag="PLS64_05160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
     gene            complement(7488..7655)
                     /locus_tag="PLS64_05165"
     CDS             complement(7488..7655)
                     /locus_tag="PLS64_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43252.1"
                     /translation="MASDFKLFKKKDDKKNNGQNFSLSDKSKFAIVESYKAARTNIMF
                     SLSAKEKNALP"
     gene            complement(7665..8396)
                     /locus_tag="PLS64_05170"
     CDS             complement(7665..8396)
                     /locus_tag="PLS64_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRL43253.1"
                     /translation="MAKNVTIQRIVGVLLQRIKFIILATVVMGLLFFMYSRFVIAPMY
                     STSTMIYVQNYSSSQRANANADTKSTTPSKAADDTTKKTTNEENQKIYPADISASANL
                     AEICVTLFKNSDEMTALYDGCTVNVDVTEGTFFITITVDGTDAQKCANVANQLAEKAA
                     EVYNSKFSYGQIGTLRQAKVPSAPYAPSNTKNMLIGAAIGLIASCLISILIELIDTTI
                     KSEDDIQDIYGIPIFAEIPDFENQG"
     gene            complement(8649..>8838)
                     /locus_tag="PLS64_05175"
     CDS             complement(8649..>8838)
                     /locus_tag="PLS64_05175"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRL43254.1"
                     /translation="GKVNPLIGSAGVSAVPMAARVSQKVGQAENPGNFLLMHAMGPNV
                     AGVIGSAVAAGVLLNVVG"
BASE COUNT         2658 a         1876 c         1740 g         2564 t
ORIGIN      
        1 gaactcgcct ccattaaaga aagcgagttt ccgttagttg aaaatatttt gtaactctta
       61 cacagaatta ttctttttaa ttttggaatt gataaattcg accagtcgga atatgaggaa
      121 aacggcggca ttgactatta caacgcttgc acctgtcggg gtatcgcatg aatatgacac
      181 gcacataccg acaaagaaac aacagagtga aagcacaccc gaacagagaa ttaccgactt
      241 gaatttttta cagactctca tggaagaaag tgcagggaaa attataagtg cggaaataag
      301 caaagttccc ataattctca taccgattac aattgtgaga gccgtaagga gtgcaattac
      361 cgtgttataa acgcctgttt taagaccggt tgcccttgaa aatgtttcgt caaaggttac
      421 ggcaaaaatc ttgttgtaga acaagatgaa tattacgatt acaacaagtg caaggattcc
      481 gcacataatt gcgtcggctg ttgtagtggc aagaatactg ccgaaaaggt atgagttgag
      541 gtcggtattt acgcccgata cactgacaaa tacaacacct atggcaagcg ccgtacttga
      601 aattattgcg attgccgcat cgccgttgat tttactgttc tcggaaagtc ttaacagcag
      661 gaatgccgca ataatgatta caggaacagc aacttcaagc ggtgcaagat ttaatgccgc
      721 cgctactgca agcgcaccga agcccacatg agataatccg tcaccgatca ttgaataccg
      781 tttgagaacg agcgttacac cgagcagtgc cgcacacaag gaaacaagcg tgccgacaat
      841 gaatgctctg actataaaat tgtatgaaaa catttcaaaa atcatatcaa acatttttag
      901 cacctccgtg aactctgtgc tgacattcat cacacgggca atcgctgaga ataaagcgtt
      961 tgccggcatc cgaatgcaga tactgatgtg ccgtaccgta gaaataccaa ttctttgcaa
     1021 ggtgcagaat ttttgaggca ttgctcacgg ctgaattcac atcgtgagaa accataataa
     1081 tcgtgattcc gctttcattc aactttttga tgagagcgta catatcagcc attgccatcg
     1141 gatcaagtcc cgttacaggc tcgtcaagaa taatcagttt gtgagttgca cacaatgctc
     1201 gggcgagcat gacacgctgt cgctgaccgc ccgaaagctc tctgaaacat tttttgcgta
     1261 aatcataaat gcccgtgagc ttcatattct cggcggcagt ttttttctgc tcctttgaat
     1321 agaaaaaggt gtgtttttta ttaagacagc ccgaaagcac cacctcttgc accgacgcag
     1381 gaaagtcacg ctgaatatcg cttgactgcg gaaggtagcc gatttcattc tgttttagtc
     1441 cgtcactata ggtaatcgaa ccgctcaatg gctttaaaag tccgagaagt gctttcatga
     1501 gagtagtttt gcccgaaccg ttttcaccaa ctatgcagat ataatccttt tcttttatat
     1561 cgaccgaaat atcattgaca acggttttgc cttcatagcc gagcgaggca ttttcaattt
     1621 taataagcga catcagtata acgcctcctt taatgcttca agattttgtt ccataacaga
     1681 aatataagtt gcaccgtttt caaaatcatt ctttgtgaca ttgtgacatg aagaaaactg
     1741 catcgtcttt gcagaagaat cttcagagat aatccgagca attttgccgc ttgaaagctc
     1801 aagatagaat acaacgggta tatcgtgttc tcttgtaaaa tcaatcaagt gagaaacaac
     1861 cgccgtactc ggctcggttt catgactgca acccgggaat gcacattcat atccaaggtt
     1921 gtagtcgctt acaaaataca gaaacggaaa tctgtcgccg aaaacaagcg tatcacgctt
     1981 tttgttattt gcaacttcgg taaatttctt atcaaggctg tcaagctgtg aaatgtaatt
     2041 tgtttttctg tcattgtaat atttagaatt tttgctgtca actgacatca actcatctgc
     2101 gattgaggca gacatacgct ctgcatttct gattgaagtc cagacatgct cgtcatactc
     2161 ctccccttct tcgtgatcgt gaccttcgtc atcatcatcc atatcttcat gactgtcgtg
     2221 ttcatggtca tgatccatag attgttcatg tctgagagga acatactcca tcatcttcat
     2281 cacttttaca tctttgtttt caattgacga aagaactccg tcaacccatt cgtcgctttc
     2341 accgccgttg cagataaaaa tatcgcaatt ttcaattgct gtaatatctg acggtttggg
     2401 ttcataactg tgcggatcgc ttccgggcga aagcaacagg cgaatatcaa ctttatcgcc
     2461 tgcaagctgt cttacaaaat catagtaagg aaaaatcgtt gttacaacgc tgattttacc
     2521 gtcagatttc ctgacatctt cagttctgtc acaagcggaa attaataaca agccgcaaat
     2581 tatcacggca gaaagaaaaa ttgaaacaag ttttttcata tatcactcca aataacgctt
     2641 aaaaagtctt aatacacaaa agggattgca taaaacatct ttatgcaacc cctttataat
     2701 tatttgcaga atcggataaa tttatccgaa agctgtttta tgcagcttta gcggcaagct
     2761 tctttgcctt tttatgacgc tgccacataa cccaaagcgg acctgcgata cagattgaag
     2821 aataacaacc tgatatgata ccgataatca tcggaagagc aaagccctga acagatgtga
     2881 tattgaaagc acaggcaaca acataaacaa caagaatagc cgaaagtgtt gttacagaag
     2941 tcataattgt tctcttaata cactttgtac agctgaggtt gaatatcttg ccaacatctg
     3001 ttttgcttcc gacaagcttc ttttcctcac gaacacggtc atagataaca attgtatcgt
     3061 taagcgaata accgagaatc ataagaacaa ctgcgatgaa gcttgcgtca atcggcatct
     3121 ggaagattac atacataaag taaatcattg caacatcgtg gaagagggct acgagagcca
     3181 taacacctgc cgaaagaccg ccgattttct taaatctgat tgttacataa agaaccataa
     3241 gcacggctgc aattgcaaca gcagtaagac acttgagaag gaagctgaaa cccattgaag
     3301 catcaactga atttgattca agacatttga agttattgtc cggatacttc ttctgaaggt
     3361 ctgattcaag gttcttctga atatcggttt tgattgaatc gttacccgag aactgaactg
     3421 ttaccgttgt gccgctgtca cccataagat cctgagcata tgtagtagtg attctgtcct
     3481 ctgttgtttc aacctgaata aaatcagtaa gttcagactg attgatttca ccgctgtagc
     3541 tgaatttaag tgtggcaccg ccagagaact gaatgtcaag ctgtgtaccg aaaataaagt
     3601 tacatacgat accgataata ataattgcaa gagaaattcc gaagaaaatc tttttgtgac
     3661 ctacaaagtc aataatcttg tggcctctgt tatacttttc ggcaaattcc tcatctgtaa
     3721 ggggggcatt tgaaactgcc tttgtctgag ttttatcagt cattcttagc acctccaaac
     3781 aaccattttt tacgcaggaa cttaaagcct gcaacacttc tgagcataat tcttgtacag
     3841 aatacaccca tgataaagtt agcgattacg cctacaagaa gtgtgtaacc gaatgagtag
     3901 atagtacctg ttgtagactc accgaagatg attgagagaa tatttgcagg accaaataca
     3961 agcataagga taattgatac gataataacg gtcatattac cgtcaacgat agcggaaagt
     4021 gaattctttg tacccctttc aagagcaccg tcaagagttc tgcccgcttt aagttcttct
     4081 ttgatacgct cggcagtaat aacattacag tcaacgccca taccgattga aaggataaga
     4141 cccgcaatac ctgggagcgt cattgtgaat gaagagaata cagggaagta acctgataca
     4201 gccgcaatgg caagacccat ctgaccgaga agtgagataa ctgcaacaaa accgggcaaa
     4261 cggtagaaga gaatcataat gattgcaata atgataaatg cgataacacc cgcatatgcc
     4321 attgcaacaa gtgagttctc accgagggtt gcgctgattg tgccgtagtt tgatgtttca
     4381 agctggaacg gaagagcacc tgcattaatt tttgatgcaa ggtcctgagc ctctttagct
     4441 gtaaagtcac ctgaaataac agcgttgccg tctgtaattt cagcctcaac cttaggagct
     4501 gaaagcataa tatcatccat ccagattgaa acctgctggt ttagatattc tttagtgatg
     4561 tcagcaaaac gctccttacc gttcttgtca ccgctgctct tcagcttaag ctgaactaca
     4621 taggaatttt tacctgtggt cttgtctgtt gtcatacctg ctgttgcact ttcaacataa
     4681 gaaccgtcca taaggattgt cttagctgtg tcgcccgaag gagttttgta aacatacccg
     4741 ccgtttgaat caacatctgt tgtttcatga ccgtctccgg gacggaatgt aagctgtgct
     4801 gttgaagaaa tctcttcgat tgcctcaatt gcgttctggt cggaatcttc cttccatggg
     4861 aaacgaacaa tgattctgtc gctttttgca tccgcataaa gctcataatc tgtgataccc
     4921 tgagatacca ttcttaattc aatgattgac tttgcagact caagctgctc gtcagttgcg
     4981 tcaacatctc ctgccggttt gaatgttgct tcaacaccgc cacggatgtc aattccccat
     5041 cgtatatcgc tgatgccctt taatactgtt gttttgttat cgccggtgta gtaggaaata
     5101 ccaaaaatcg cagtaaatga aaaggcaagt atcagcaaag ctacgacaaa gaatacaggc
     5161 tttggaactc ttttcatgcc tttgaaacct ccgttaaata attggctgtg attttatttt
     5221 gctcaaaagt caaaataact ctgtcacgaa catccaatca cagaccgtaa cagagttatt
     5281 atagaatttt tttaggaaaa tgtcaatgga tacggaaata tttaataaaa aaagaattga
     5341 atttatcaaa aaatacattc taatgtcata aactgcatac aaaattcttc caaaatccaa
     5401 ctgacaaacc aaattttcaa atgatgatta cttgttcaga atcttttcgc aagctgcaag
     5461 ccttgcacag atacaaaaaa catccatagc aacttcaagc tcgtggttcg gagggaaggt
     5521 cggtgcaatt ctgatgttgg aatcctttgg atcaattccg agcggatatg ttgcacctgc
     5581 acccgtaaga acaactcctg cctccttaca gagagcgact gttctctttg ccgtgccttc
     5641 aaaaacatca acgcttacaa agtaaccgcc gtgcggcttt ttccatgatg cgatatcagc
     5701 aggtgcaagt tctttgtcga gagtatcaag aacaatctcg aattttggtt taaggacaga
     5761 cttgtgcttt tccatatgtt taagcacgcc gtcatagtcc ttgaagaatc ttacatgacg
     5821 gagcatatta agcttatcat agctgatgat ttcgtagtta tatttttcac ggagaacctt
     5881 gaggttgttt tcagatgcag ccattgcggc tacgcctgca ccggggaaag taatctttga
     5941 tgttgagcag aaaagaatcg ggaggtcttc attacctgtc tttttgcatt catccataat
     6001 gttgagcaaa acatcaggtg tgtcgctgat ttcatggatg cagtaagcat tatcccacat
     6061 aattctgaaa tccttagcgg caggctttaa agcggcaaag cgcttaacaa cctcatcgct
     6121 gtatgtgatg ccggcagggt ttgaatattt cggaacgcac caaataccct tgattgagtc
     6181 atcctcagaa acgagtcttt caaccatatc catatcggga ccgttgtcgt ccatcggaat
     6241 cggaataagc tcaaagccgt aatactgagt aatgccgaaa tgacggtcat aacccggtac
     6301 cggacagagg aattttctgt tctttacttc ataccatggt ttgcagccct caacagcggg
     6361 agctgtcata aagcatgaaa ttgtatcaaa catcatatta aggcttgaag aaccgcctac
     6421 aataacattc tctgcatcta caccgagaat atccgcaaaa agctttctgc actctgtaat
     6481 accgttgaga agtccgtagt tgcggcaatc catcttatcc tcaccgataa aatcggattt
     6541 gctgttgatt gtatcgagca taggtaagga aaaatcaagc tgttctttgc tgggcttacc
     6601 tcttgccata ttgaggttaa gtcccatacc tttaatctcc tcaaatctct ttacgagatt
     6661 gccatactct ttttcaagtt cctgcttact caagtcaaga taacccattt tgaataactc
     6721 cttaaaatat tattgcaaat gcaatttaaa tttctaatat attttactct attcgcccaa
     6781 gaaatgcaag atgttttttg caatcttgca tttttatttt cacaaaaaag aacagacctg
     6841 caacagtcat ttttggtgaa agtttgcaag tctgtgattt tgtgttattt ttatcgagaa
     6901 tgtgtagttt tgtgccaaag cacaaaattg ggattaataa tactggtagt agtaataacc
     6961 tttctttctt ctctttgaat cataagtaca acctacctca atcagaccga gtacggttga
     7021 atcggcaagc tttacgctgt caagaaggtg ctgaaggtca ccgtgagttg ttgagcgttc
     7081 acgaacaact acaacaaagc cggcaatcat atccttgaga agcagagcgt cggcaaccac
     7141 accgatagga ggcgtatcaa aaattacata atcataatct ctgacaaact gctcataaag
     7201 ttcctgaaat ctggtactgc acataagctc agaggggttt ggcgcaaccg tacctgccgg
     7261 aagaatgtca agattcggaa gaacatcatg cttaacagcc tcatcaattt caatcatttt
     7321 accgattaca ttggtaagtc cctttttatt gtcaagctta aaaatgttgt gaacattagg
     7381 acggcgaagg tcgcagtcga taaggagaac tcttttttcc attttcgaaa aggaaattgc
     7441 aagatttgcg gaaacggtgc tcttgccctc accctttgag taactggtta cggcaaagca
     7501 tttttttctt ttgcggaaag cgagaacata atattagttc ttgcagcctt atacgactca
     7561 acaattgcaa acttgctctt atcggaaaga gaaaaattct gtccgttatt ttttttatcg
     7621 tctttctttt taaataattt aaaatcagaa gccatagttc ctccttaacc ctggttctca
     7681 aagtcgggaa tttcggcaaa tataggaata ccgtagatat cctgaatatc gtcctcagac
     7741 ttgattgttg tgtcgataag ttcaataaga atagaaataa ggcaggaagc aattaatccg
     7801 attgcggcgc ctataagcat attctttgtg ttactcggtg catacggagc cgaaggaacc
     7861 ttcgcctgac gaagtgttcc gatctgaccg tagctgaatt ttgaattata aacctcagcc
     7921 gccttttcgg caagctgatt tgccacattg gcacacttct gtgcgtctgt accgtccact
     7981 gtaattgtga taaagaaagt accttcggtt acatcaacat taaccgtgca gccgtcatag
     8041 agtgctgtca tctcatcgga atttttaaag agtgtaacgc agatttcagc aagatttgca
     8101 gaggcggaaa tatcagcagg atatatcttc tggttctcct cattggttgt ctttttggtt
     8161 gtatcatcgg cagcctttga aggtgtcgta ctcttggtgt cggcattggc atttgctcgc
     8221 tgtgacgaac tgtagttctg cacataaatc atggtagatg tgctgtacat cggtgcaatt
     8281 acaaagcgtg aatacataaa gaacagcagt cccattacaa cagtagcaag aataataaac
     8341 ttgattcttt gcaaaaggac gccgacaatt cgctgaatgg ttacattctt tgccatttat
     8401 atagcccctt taattcatat taagtattaa gaaaacatac cgtaaagcaa aaaaggtgtt
     8461 tttacggcac tgatttacac atttatattt tattatctta tgtattttca gcaataattc
     8521 tatcatagaa tattgattga aataatttct ttaatattat acaaattttt cgttgtcgga
     8581 ttctttaaca acaaaaataa aggaagccgt aaagacttcc tttatcatag tttattaagt
     8641 tacggttatc agcctacaac attaagaagt acgccggctg caactgctga accgattaca
     8701 cctgcaacat tcggacccat agcgtgcatg aggaggaagt tgccgggatt ttcagcctgg
     8761 ccaaccttct gagatacacg ggcagccatc ggaacggcgg atacgcctgc tgaaccgata
     8821 agcgggttaa ccttaccg
//
LOCUS       DAOMVE010000069         8696 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2090_length_8696_cov_2.978012, whole genome shotgun sequence.
ACCESSION   DAOMVE010000069 DAOMVE010000000
VERSION     DAOMVE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2090_length_8696_cov_2.978012"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..343
                     /locus_tag="PLS64_05180"
     CDS             101..343
                     /locus_tag="PLS64_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRL43255.1"
                     /translation="MRLDKYLKVSRLIKRRTVANEACDAGKITVNDKVARASYDVKVG
                     DVIEITLGSKSVKVKVTEVKEVVRKEDAATMYEAAV"
     gene            679..862
                     /locus_tag="PLS64_05185"
                     /pseudo
     CDS             679..862
                     /locus_tag="PLS64_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013655147.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            925..1197
                     /gene="yabP"
                     /locus_tag="PLS64_05190"
     CDS             925..1197
                     /gene="yabP"
                     /locus_tag="PLS64_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HRL43256.1"
                     /translation="MQEMPKAKNHTVMLDNRGKLVMTGTEDVSGFNEETVSVKTSCGC
                     LVIKGENLHIDKLNLETGDVSIDGKINAMQYLGNGASKSKLSKLFK"
     gene            1672..2046
                     /locus_tag="PLS64_05195"
     CDS             1672..2046
                     /locus_tag="PLS64_05195"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRL43257.1"
                     /translation="MKKSTKKVKESTAASRQTEDGAIKEITPKKRKKGRYILIYLAII
                     GFVFYAVITIINQNVQIAEKKAELTDLQQQISVVEIQSQYLKKVQNYKGDDLKKYIEK
                     IAKDELGYVGDGERIFINVAGE"
     gene            2083..2574
                     /locus_tag="PLS64_05200"
     CDS             2083..2574
                     /locus_tag="PLS64_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRL43258.1"
                     /translation="MAIEVGMILEGKVSGITKFGAFVDLPDSKTGMVHISEVAPTFIN
                     EISDYVKVGQEVKVKVLALNDGKISLSMKQALPKEQQQQRKRSDRPQRQNHRSREPYK
                     PAPPVTSPGDYEWQSSRRNAPSSFEDMMSKFKQTSEDKMSDLKRGGESRGYSRRGNGN
                     GRK"
     gene            2604..3140
                     /gene="raiA"
                     /locus_tag="PLS64_05205"
     CDS             2604..3140
                     /gene="raiA"
                     /locus_tag="PLS64_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRL43259.1"
                     /translation="MKITYTARKVNLRDNFKERCEKKLSKFNRIFSEDASVNVVVTLE
                     KNRQTVEITIRDNGMIYRAESTMPEMNDALDKVCDILMRQIRKNKTRLEKKIKSAGIE
                     DLITEAAQTDEPEDDEYSVVRKKHVLVKPISVDEAILEMNMVNHNFFMFINSETDEIN
                     VVYKRADGNYGVLEPTTD"
     gene            3237..4352
                     /locus_tag="PLS64_05210"
     CDS             3237..4352
                     /locus_tag="PLS64_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRL43260.1"
                     /translation="MNMKFSAPCIFGLEGLCANELRYLGVENVNAENGRVTFTGDWST
                     LALANINSRYAERILILLAEFEVHTFDNLFEGVKAIPWEDYIGISEAFPVKGRCLDSD
                     LMSVPDCQKIVKKAVADRLSQKYMLPWFDETGAPHQIQFLILRNKVSIMLDTSGAGLH
                     KRGYRADSNDAPIKETLAAAMVDLSRVRANHFVTDPMCGSGTILIEAAMKALNIAPGL
                     NRYFACEHWNCVPKDVFETARENARQKIRHDATFRATGYDIDDSALAIAKKNAEIAGV
                     ADRITFANRDIKDFELEDGFQTIITNPPYGERLLDVKSAEKLYAVMGEHFKRMKGKSY
                     TVISPDDDFEKIFGRKADKRRKLYNGTLKCELYMYFK"
     gene            4449..5396
                     /locus_tag="PLS64_05215"
     CDS             4449..5396
                     /locus_tag="PLS64_05215"
                     /inference="COORDINATES: protein motif:HMM:NF037491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5640 domain-containing protein"
                     /protein_id="HRL43261.1"
                     /translation="MDENKEILTSENEAENTNTDTTAETTENTTFVADEPTESEEMSF
                     AETEPVNENGVTAPQPKKKSIIQLPIIIGIVIVAVAAATLFVIKGFFDTSIVGTWIES
                     VDVTGNSASSDEASKVDVYYTFNGDNTLNYRIGSMVWNGTYTVSTDDSGNQTLAMTLN
                     GNETSCNYAVSGNMFSGRTLELSTSTAANKVKLKSGSEVKPELKVDKDFKADKKITGS
                     WTYDNGYAKMTYKFNDDGTVEINQSDALNVDGTYLIKNGTIIIKYYYTDEVEMDVAYS
                     VESNGLKLNDILYTKDDDSAKTTAAVTEAATETATTSAK"
     gene            5480..6058
                     /locus_tag="PLS64_05220"
     CDS             5480..6058
                     /locus_tag="PLS64_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HRL43262.1"
                     /translation="MTPQEEFIEIYNKYITRQGADELLEWLKRTDFFTAPASSKYHCA
                     CENGLVMHSVSVFNTMMEKHYDEETDNIESFAICGLLHDLCKAQFYKVSSRNVKNEKT
                     GQWEKVPYYAIEDQFPYGHGEKSVFLIERKMHLKIDEAMAIRWHMGEFGDKNSNTISQ
                     AYDKYPLAVKLHLADLESTFLREKGTSQVNNR"
     gene            6121..8106
                     /gene="ligA"
                     /locus_tag="PLS64_05225"
     CDS             6121..8106
                     /gene="ligA"
                     /locus_tag="PLS64_05225"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRL43263.1"
                     /translation="MDFITAQNRAQELTKQLEYHNNLYYNQDDPEISDYDYDMLMREL
                     ENIEKEFPSLKSPMSPTNRVGGNAGEKFSPVTHEVVMESLHDSFSHEELREFDARVRE
                     TVPNVRYVVEPKFDGLSVSCEYENGVFVRGSTRGDGSVGEDVTENLMTIKSLPKRIPN
                     APEYLEVRGEVYMSFKSFDALTRRQEENEEKTFKNPRNAAAGSLRQKNAKITAQRSLD
                     IFVFNIQQIRGMTIESHIQGLDYLTELGFPTAFYSVYDNVDEVIEEIERIGNMRGELD
                     YAIDGAVVKVDSFASRRLLGSTAKYPKWAEAYKYPPEEKPTKLLNIEINVGRTGVLTP
                     VGNFEPVLLAGTTVSRATLHNKDFIDEKGICVGDTVIIRKAGEIIPEVLSVKEHAENA
                     VPFEFPSVCPSCGNPVTHDEGEAAIRCTNTDCPAQLTRHLIHFVSRDAMDIDGLGPAI
                     LEQLSDEGLVKSPADLYRLKAEDISSLERKAEKSAQNLISSIEKSKENELFRLVFALG
                     IRNIGLKAAKIICENFATIDDIMSAKAEDFEKIDGFGAVMAESLENYFSLDGTKELIA
                     DLKSLGLRMKPSQPKNTGGLFEGKTFVLTGTLPTMTRSEASKLIEQNGGKTSSSVSKK
                     TSYVLAGEDAGSKLTKAQTLGVTIISEEELKEMLSME"
     gene            8169..8657
                     /locus_tag="PLS64_05230"
     CDS             8169..8657
                     /locus_tag="PLS64_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43264.1"
                     /translation="MKKYLILAVVTLLAVLLSACSGNVKSNDTSAENTTTQVSTSVNE
                     TAQTSNAELPKIYNPTNVEIRDNEDENKVIIESSQIEYVCLLDDINGMVLNMKLTADG
                     TDKLADYTKNNIGSAVRFVINGKTVSNPYINVEITDGNINFTGDYTNEEYAAIFSEIK
                     SK"
BASE COUNT         2877 a         1569 c         1851 g         2399 t
ORIGIN      
        1 gctttaagga tgtagtaaac aagtaatctt gttttatatt tcgggtaatg gtacgctgta
       61 tcattaccct tttttattaa aaatcggaag ggtttataat atgcgacttg ataaatattt
      121 aaaagtatcc cgactcatca aacgcagaac agttgcaaat gaggcgtgtg acgcaggcaa
      181 aatcacagtt aatgacaagg ttgcccgtgc gtcctatgat gtaaaagtcg gtgatgtaat
      241 cgaaatcaca ctcggttcaa aaagtgtaaa ggttaaggtt acagaggtaa aagaagttgt
      301 tcgcaaggaa gatgcggcaa caatgtatga ggcggcagtc tgatttttta ccaaaattaa
      361 aacacaaaat attactttgg atgaaattag gtgagtagat tatgaataag acaattaatt
      421 taatattggc aatattatct gtaattactt acattgggat tattgtaatg aagttttaca
      481 cacagataaa tatacccaat ggctctttcg taataatgac tttattgttc attatttcgg
      541 tatgccaatt tttaaattcg ttaaaataat ctacacacga ggagaatatg tgtataccac
      601 gacattcatc aggtttacta gtaggaataa ttatcaactt aataaatata aataaatcgt
      661 aagttgaggt attaaactat gaaatattgt tgtgttaaac aatgtgatat aactgattgt
      721 ggtgcttggt tttacggcaa agggtgtaaa aggcaatcag gaggcgttct tctctgaatt
      781 ccctcttctt gctattgctc atgttattgt tgatggcaat ctgatgcact atgttgttat
      841 acataaaatc tcaaaaaaag aagaataatt ttattttttt ctccgtataa attactgaca
      901 aataatttat acggaggttt ttttatgcag gaaatgccaa aggctaagaa tcatacggtt
      961 atgcttgata acagggggaa gcttgttatg acaggcacgg aggatgtgtc gggcttcaac
     1021 gaggaaacgg tgtcggtaaa aacctcctgc gggtgccttg taatcaaagg ggaaaatctg
     1081 cacattgata agttaaacct tgaaacggga gatgtttcaa ttgacggaaa aattaacgca
     1141 atgcaatacc tcggcaacgg tgcgtcaaaa tccaaacttt caaaactctt taagtaggtg
     1201 taatcttgga gctgactttt gcacagcagt caaccgcatt tctctgttca cttgtattcg
     1261 gagcggcggc aggattgctg tatgatatat ttaaaataat cagaatgacg ctttgcaaag
     1321 gaaaagcgtc ggtttttgtt acagactttc tttatgtttt tattgtatca cttaatctgt
     1381 tcattttttc cgttgcatat atgctgggat ttttcagagt gtttgtaacg gtcggttcat
     1441 ttctcggttt tgttatttgt cgattaactc tcggcagact tttatcgctt gtatattgtc
     1501 cgatgattcg tttcacggag agtgtctgtg tgaaaatttc gcaaaaaatt aaaaaaaatc
     1561 taaaaaagct attgaaaaac agcaacaaca tattgtataa tgagggtaag aataaaggta
     1621 tatttagaaa cgataataat aattcagcag agaaagcaga tagttttgaa gatgaaaaaa
     1681 agcacaaaga aagtaaaaga aagcactgcg gcttctcggc agacagaaga cggcgcaata
     1741 aaggagataa ctccgaaaaa gcgtaaaaaa ggaagatata ttctgattta tctcgcaatt
     1801 atcggttttg ttttttatgc cgtaattaca attatcaatc agaatgttca gattgccgag
     1861 aaaaaggcag aacttacgga tttgcagcag caaatcagcg tggttgagat tcagtcgcag
     1921 tatctgaaaa aggttcagaa ttacaagggt gacgatctta aaaaatatat tgaaaaaata
     1981 gctaaggatg aattaggcta tgtcggtgac ggtgaaagaa ttttcattaa tgttgccgga
     2041 gaataaaagg gtagaaataa ttttaagggg taataaattt ttatggctat tgaagtcgga
     2101 atgattctgg aaggcaaagt atcaggtatt acaaaattcg gcgcatttgt tgatttgccg
     2161 gattcaaaaa ccggtatggt acatatctcg gaggttgcac ctaccttcat caatgaaatc
     2221 agtgattatg taaaagtagg acaagaagta aaggttaagg tgcttgctct taatgacgga
     2281 aaaatcagtc tgtcaatgaa acaggctctc ccgaaagaac agcaacagca gagaaaacgc
     2341 tctgacagac ctcaaagaca gaatcacaga agcagagagc catacaagcc tgctccgccc
     2401 gtaacttcac cgggtgatta cgagtggcag agttcacgca gaaatgctcc ttcttcgttt
     2461 gaggatatga tgtcaaagtt caagcagaca agcgaggata agatgtccga tcttaagcgt
     2521 ggcggagaaa gccgtggcta cagccgcaga ggcaacggca acggtagaaa ataaaaatat
     2581 aatatataag ggaggcagat tttatgaaaa tcacctacac agcaagaaaa gtcaacctca
     2641 gagataactt taaggaaaga tgcgagaaaa agctttcaaa gtttaatcgt attttctcgg
     2701 aggatgcttc tgtcaatgta gttgtcaccc tcgaaaagaa ccgtcagact gttgaaatta
     2761 caatccgtga caacggtatg atttatcgtg ccgaaagcac aatgcccgaa atgaatgacg
     2821 ctcttgacaa ggtttgcgac attcttatgc gtcagattcg taagaataaa acgaggcttg
     2881 aaaagaaaat taaatccgcc ggtattgaag atttaatcac ggaagcagct cagactgatg
     2941 aaccggagga cgatgaatac agcgtagttc gtaaaaaaca tgttcttgta aagccgattt
     3001 cggttgatga agctattctt gaaatgaata tggtaaacca taacttcttt atgtttatca
     3061 attccgagac agatgaaatc aatgttgttt acaagcgtgc agacggcaac tatggtgtgc
     3121 tcgagccgac aaccgattaa ttttttaaaa gcaaggggta tcggttattc cgataccctg
     3181 tttttttgtc tttcagtctt aatagaattt gtgtatcaat aaaacaggag ataaatatga
     3241 atatgaaatt ttccgctcca tgcattttcg gtcttgaagg tctttgtgca aatgagctga
     3301 gatatctcgg tgttgaaaat gtcaatgccg aaaacggcag agttaccttt acaggcgatt
     3361 ggtcaacact tgcacttgca aatataaatt cccgttatgc ggagcgaata ctcattcttc
     3421 ttgcggaatt tgaggttcac acctttgata atttgtttga gggcgtaaag gctatcccgt
     3481 gggaggatta tatcggaatt tccgaggctt ttcccgtaaa gggaagatgt cttgattccg
     3541 accttatgag cgttcccgat tgtcagaaaa tcgttaaaaa ggcagtcgca gaccgccttt
     3601 cacaaaaata tatgctcccg tggtttgatg aaacaggtgc tccgcatcag attcagtttc
     3661 tcattcttcg caacaaggta agtattatgc ttgacacttc gggcgcagga cttcacaagc
     3721 gtggatatcg tgccgattca aatgatgctc ctataaaaga aacacttgcc gccgctatgg
     3781 ttgacctttc aagagtaagg gcaaaccact ttgtaaccga tcctatgtgc ggcagcggca
     3841 caattctcat tgaggcggct atgaaagcac ttaacattgc cccgggactt aaccgttatt
     3901 ttgcctgtga acattggaat tgtgttccga aagatgtttt tgaaacagca agagaaaatg
     3961 caagacagaa aataagacac gacgcaacat tcagggcaac aggttacgat atagatgatt
     4021 cagcccttgc aatcgcaaag aaaaacgcag aaattgcagg agttgccgac agaataacat
     4081 ttgcaaaccg tgatattaaa gattttgaac ttgaggacgg ttttcagaca atcatcacaa
     4141 atccgccata cggtgaacga ttacttgatg taaaatcagc tgaaaagctg tatgcagtta
     4201 tgggtgaaca ttttaaaaga atgaagggta aaagctacac cgttataagc cccgatgacg
     4261 attttgaaaa aatcttcggc agaaaggctg acaaacgccg caaactttat aacggaacgc
     4321 ttaaatgcga actttatatg tattttaaat aattttcatt acattttaac ggcataaaaa
     4381 acagttgatt ttctttcata tttatagtat gatagaaagc gatgacaaaa tttaaggagg
     4441 tatcacagtt ggacgagaat aaagaaattc ttacttctga aaatgaggca gagaatacca
     4501 acacagatac aacagcagaa acaacagaaa atacaacatt cgttgcagat gagcccacag
     4561 aaagcgaaga aatgtctttt gctgaaacag aacctgtaaa cgaaaacggc gtgactgctc
     4621 cgcagccaaa gaaaaagagt attattcagc ttcctatcat tataggtatc gtaattgttg
     4681 cggttgcggc agctactctt ttcgtaatca aaggattttt tgatacaagc attgtaggaa
     4741 catggattga atctgttgat gtaacaggca attcagcatc atccgacgag gcgtcaaagg
     4801 ttgatgttta ctacacattt aacggtgaca atactctcaa ttacagaatc ggttcaatgg
     4861 tttggaacgg tacatacacg gtttcaacag atgattcggg taatcagaca cttgccatga
     4921 cgcttaacgg caatgaaaca agctgtaact acgctgtttc gggtaatatg ttctccggca
     4981 gaactcttga gctttcaact tcaactgctg ccaacaaggt gaaactcaag tcaggttcag
     5041 aggttaagcc tgaattgaag gttgataagg acttcaaggc tgacaagaaa atcacaggtt
     5101 cgtggactta tgataacggt tacgctaaga tgacatacaa gttcaatgat gacggtactg
     5161 ttgaaatcaa ccagtcagac gcactcaatg ttgacggtac ttacttaatc aagaacggca
     5221 caatcatcat caagtactat tacacagatg aagttgaaat ggatgttgct tattccgtag
     5281 agagcaacgg actcaagctc aatgacattc tctatacaaa ggatgacgat tcggcaaaaa
     5341 ctacagcagc agtaaccgag gcggctacgg aaacagctac aacttcagca aagtaaatct
     5401 tttgaacttt taatgcaggg caacggcaga ttatctgctg ttgccttttt aaaaatacaa
     5461 ttatatgagg aggattaata tgacgccgca ggaagaattt attgaaatct acaacaaata
     5521 tatcacaaga cagggtgctg acgagcttct tgaatggctc aaaagaaccg atttttttac
     5581 cgcccctgca agttcaaaat atcattgtgc ctgtgaaaac ggtcttgtaa tgcactccgt
     5641 cagcgtgttc aatacaatga tggaaaagca ctatgatgaa gaaaccgaca atattgaaag
     5701 ttttgcaatc tgcggacttc ttcacgattt gtgcaaggct cagttctaca aggtttcaag
     5761 cagaaatgtt aagaatgaaa aaacaggtca gtgggaaaaa gttccgtatt atgcaattga
     5821 agatcagttt ccctacggtc acggtgaaaa gtctgttttt ctcattgaac gcaaaatgca
     5881 ccttaaaatt gatgaggcta tggcaattcg ttggcatatg ggtgaattcg gtgacaaaaa
     5941 cagcaacaca atcagtcagg catatgacaa gtatccgctt gccgtaaagc ttcaccttgc
     6001 agaccttgaa tcaaccttcc tccgtgaaaa aggaacttca caggttaata acaggtaata
     6061 ttttaaacac aggtgcagat acttatttta ccgataaatt tatgatatgg ggaaaccaaa
     6121 atggatttta taacagctca gaatcgtgcc caagagctta caaaacagct tgaataccac
     6181 aacaaccttt actacaatca ggatgatccg gaaatctccg actacgatta cgatatgctc
     6241 atgcgtgaac ttgagaatat cgaaaaggaa tttccgtcac tcaaatctcc tatgtcaccg
     6301 acaaaccgtg tcggcggtaa tgcaggagaa aagttttctc ccgttactca tgaggttgta
     6361 atggaaagcc ttcacgatag tttttcacac gaagagcttc gtgaatttga cgcaagagtg
     6421 cgagaaaccg tgccgaatgt acgctatgtt gttgaaccta aatttgacgg tctttccgta
     6481 tcgtgcgaat atgaaaacgg tgtttttgta agaggttcaa cccgtggcga cggctctgta
     6541 ggtgaagatg taaccgaaaa tcttatgaca attaaaagtc tgccaaagcg tattccgaat
     6601 gcacccgaat atctcgaggt tcgtggtgag gtgtatatgt cgttcaagtc gtttgatgca
     6661 cttactcgca gacaggaaga aaacgaggaa aaaaccttta aaaatccacg aaatgccgcc
     6721 gcaggctcac ttcgtcagaa aaatgcaaaa ataaccgctc agagaagtct tgatatattc
     6781 gtgttcaata tccagcaaat cagaggaatg acgattgaaa gccatattca agggcttgac
     6841 tatcttacgg aactcggctt tccgactgca ttttacagcg tttacgataa tgttgacgag
     6901 gtgattgagg aaattgagcg cataggaaat atgcgtggag agcttgatta tgcaattgac
     6961 ggcgctgttg tcaaggttga cagctttgca agccgccgac ttctcggcag taccgcaaaa
     7021 tatcctaaat gggcagaggc atataagtat cctcccgagg agaagcctac taaacttctc
     7081 aatatcgaaa tcaatgtcgg cagaacgggt gtgcttactc ctgtaggcaa ctttgaacct
     7141 gtacttcttg caggaacaac agtaagccgt gccacacttc acaacaagga ttttattgac
     7201 gaaaaaggta tttgcgtagg cgatacggtt ataatcagaa aagcaggcga gattattccc
     7261 gaagttctgt cagttaagga acacgctgaa aatgccgtgc cgtttgaatt tccgtccgta
     7321 tgcccatctt gcggtaaccc tgttacacac gatgagggcg aggcggcaat ccgttgtaca
     7381 aacacagact gtcccgctca gctgacaaga catcttattc actttgtaag ccgtgacgca
     7441 atggatattg acggactcgg tcctgcaatt cttgaacagc tttcagatga aggacttgta
     7501 aaatctcctg ccgacctcta cagacttaaa gctgaagata tttcaagcct tgaacgcaag
     7561 gctgaaaaat ctgctcagaa tcttatttct tcaatagaaa aatcaaaaga aaacgaactt
     7621 ttcagacttg tttttgcact cggaatccgt aacataggtc ttaaagccgc aaagataatc
     7681 tgcgaaaact ttgcaacaat tgacgatatt atgagtgcaa aggctgagga ctttgaaaaa
     7741 atagacggtt tcggtgctgt tatggcagaa agccttgaaa attatttttc acttgacggt
     7801 acaaaggagc ttattgcaga ccttaaatca ctcggtctga gaatgaaacc ctctcagccg
     7861 aaaaacactg gaggtctttt tgaaggcaaa acctttgtcc tcacaggcac acttcccaca
     7921 atgacaagaa gtgaagcctc aaaactcatt gaacagaacg gcggaaaaac aagttcatca
     7981 gtttcaaaaa agacttcata tgtcttggca ggcgaagatg ccggcagtaa actaaccaaa
     8041 gcccaaacct taggtgttac aataatttcc gaagaagaat taaaagaaat gctgtcaatg
     8101 gaatagttac aataaatact tgacactgtt tatgttgtta tataattata acgaaagggg
     8161 tacttaaaat gaaaaaatat ttgattttag ctgttgtaac tttacttgct gttttgttat
     8221 ctgcttgcag cggaaatgta aaaagtaacg atacttctgc tgaaaataca actactcagg
     8281 tttcaacctc ggttaatgaa acagctcaga catcaaatgc tgaattgccg aaaatttata
     8341 atccgaccaa tgtagaaatc cgtgataatg aagatgaaaa caaagttatt attgagtcat
     8401 ctcaaatcga gtatgtgtgt ttgcttgatg atattaacgg tatggtgtta aatatgaaac
     8461 ttacagctga cggaactgat aagcttgcag actacactaa aaataatatc ggttctgctg
     8521 ttcggtttgt tattaacgga aagacggttt caaacccgta tataaatgtt gaaattactg
     8581 acggcaatat taattttaca ggtgattaca ctaacgaaga atatgctgct atctttagtg
     8641 aaataaaaag caaataaaac aattttcaat cctccgattt atgtcggggg attttt
//
LOCUS       DAOMVE010000070         8553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2132_length_8553_cov_2.849494, whole genome shotgun sequence.
ACCESSION   DAOMVE010000070 DAOMVE010000000
VERSION     DAOMVE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2132_length_8553_cov_2.849494"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1277
                     /locus_tag="PLS64_05235"
     CDS             108..1277
                     /locus_tag="PLS64_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRL43265.1"
                     /translation="MKKVITNIYEYFRQCDAVLWLLTLTAVAYSFLLIASMQRSGEYS
                     YLRTQIIAVSVGLAASVFISIADYRFLIRKWYIAAIVGIVLAGLVFVFGVQVSGTDDT
                     AWINLPGGFSIQPSEFIKICFIITFSKHLSWLREKKMIEKFSGVLFLALHAMIPMAII
                     HIQGDDGTVLIFALMFLVMTFAAGVQLRYFVIFGGLLVCAIPIIWNVFMNEEHRNRVL
                     ALFDLDGNALTNYGWQQYQGKVSIASGEAFGSGLFNGQRVEHSIVPEQENDFIFTVVG
                     EELGFVGCMALLLILFFIILKVILNAKNSSEFEGKYICYGVFSMIASQTIVNIGMVLG
                     FLPVVGITLPLFSSGGTSALSTLISLGLVQSVRYHNTDDMNKAFVRRGSSNRIRM"
     gene            complement(1332..2591)
                     /locus_tag="PLS64_05240"
     CDS             complement(1332..2591)
                     /locus_tag="PLS64_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRL43266.1"
                     /translation="MKKKKLSLNKLFQNNKFLIILALVISTITWVYMSMGTTNDTTVT
                     ISNIPIQIELPDQLDNNGLQVFRDTEQTATVTVTGSRAVLGSIGTKDITVTGATNGID
                     SAGTYQLSLSAVKANPSANFQIISTVTPSNVNVIVDYQRETSFPIQENVVYKVADGYY
                     ASTSLASKNITISGPQTEIAKIAKVSASAELDSILNEPTSTTANIVLYDKTGNKISTE
                     LLTMEFTDVEATISVLPEKTVNVIPEFINKPKGLDLNDDMLTVEPSKILLAGPKEVLD
                     KTDSVKLESIDFATLSNKRYEYDTQGINIPSDCKNISNSTSAKLVLDLSGLSKKTFTV
                     DSFKVSGLSSKYKADVTQTSMNVTVIGSEKELKSLKSSDIEGVINTSDQSGTVGSVQM
                     PVTFKLSGTDTCWVSGSYKANITISEK"
     gene            complement(2588..3478)
                     /gene="cdaA"
                     /locus_tag="PLS64_05245"
     CDS             complement(2588..3478)
                     /gene="cdaA"
                     /locus_tag="PLS64_05245"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRL43267.1"
                     /translation="MEIFNSILSIIKTIQLRDIVDILAIALLIFGLFKLIQETRAVQL
                     LKGVIMLLIVYFLSSLFGLVMLSSLLRTFFEAAVVVIAIIFQPEIRKALEQMGRNNTY
                     KKYIKIFTKHHKGDEWKKAVEKSIVDAADTAVLFSRSKTGALLVFERETMLSDIAATG
                     TIIDAETSVALFGNIFFNKAPLHDGASIIRDGKLFAAGCILPLTSNPNVDINLGTRHR
                     AGLGISEQSDAVVLIVSEETGVISLAVNGILLREFTREELIKKLEQFLIYDRGYDDDE
                     PPKKFFQKNPKSSKREKAEK"
     gene            complement(3554..4717)
                     /locus_tag="PLS64_05250"
     CDS             complement(3554..4717)
                     /locus_tag="PLS64_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRL43268.1"
                     /translation="MIYFDNGATTFPKPLSVRKAINEAMSDYGANPGRSGHKMSVKSS
                     EIMYSCRDNAAKLFGSENPENIIFTLNCTTALNTVIKGILKQGDHAVISSLEHNAVVR
                     PLETLKKNKIEYSVAECVPYDDEQTIENFRKQFKSNTKLVICTHASNVFGIKLPIERI
                     GALCRLNGILFCVDAAQSAGTADINLSDSCIDFLCTAGHKGLYGPMGTGLLIINSETT
                     PDSLIQGGTGSDSANLNQPEILPDKYESGTPNLPGIAGLNAGIKFVLNKKTDRIYNSE
                     LGLARMLYKRLEKAENVILYTKMPEKSHSVPVISFNKNTESETVAKILNDSYNIAVRA
                     GLHCAPLAHKSFGTQDTGTVRAVVSAFTTKNDVVYFANAVSRISKNNKQKKTI"
     gene            complement(4714..4941)
                     /locus_tag="PLS64_05255"
     CDS             complement(4714..4941)
                     /locus_tag="PLS64_05255"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HRL43269.1"
                     /translation="MENNLIIFGSVTLAMKSRNLLLKNNIKSGLVRTPIHLNRKSCGY
                     SLYVPGNFERALEIIRSNGIAVKGTAAVDTL"
     gene            complement(5071..5835)
                     /gene="dapB"
                     /locus_tag="PLS64_05260"
     CDS             complement(5071..5835)
                     /gene="dapB"
                     /locus_tag="PLS64_05260"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRL43270.1"
                     /translation="MVNTAIVGCNGKMGCFVAQAVQSREDCNVLFGVDAFGESNYDFD
                     VFKTFDEATETPDVIIDFSNPAALDGMLSYAVDNKVPCVICTTGFSKDQIAKIEDAAK
                     TIPVFYSGNMSLGINLLIELSKEAAKILGNQFDIEIVEKHHNLKLDAPSGTALMIADG
                     ISEQLDEDPQYVYDRHSYRRKRSKNEIGIHSVRGGTIVGEHEVIFAGHDEVVTISHQA
                     QSKEVFAVGAVNAAVFLKDQKAGMYNMGNLLSEKMN"
     gene            complement(5884..6786)
                     /gene="dapA"
                     /locus_tag="PLS64_05265"
     CDS             complement(5884..6786)
                     /gene="dapA"
                     /locus_tag="PLS64_05265"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRL43271.1"
                     /translation="MSNHIFTGSGVALITPMKSDGSVNYDVLGDLVEFHVQNGTDAII
                     VCGTTGEAATLSEKEHCETISFVAEKTNGRIPVIAGTGSNDTSTAVMLSKSAASTGVD
                     ALLCVTPYYNKTSQQGLVRHFNVVADSVDIPIILYNVPSRTGCNIKPKTYQELCKHPN
                     IVAAKEASGDISQVALIRSLCGDNLDIYSGNDDQTVPFMSLGALGVISVFANICPKEM
                     HDICQLCLDNDFAEAQKLNFHYLELMHILFSDVNPIPVKTAMNLFGYEAGECRLPLVP
                     MSVQGYHDLKDCMEKYDLLSKKVK"
     gene            complement(6806..7891)
                     /gene="asd"
                     /locus_tag="PLS64_05270"
     CDS             complement(6806..7891)
                     /gene="asd"
                     /locus_tag="PLS64_05270"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRL43272.1"
                     /translation="MKKYKVGIIGATGMVGQRFTLLLENHPWFEVTVLAASKRSAGKT
                     YGETVEGRWHMTAPLPEKYKDMVIVDAENVEEVASQVDFCFCAVNMKKDEIRDLEDRY
                     AKAECPIVSNNSAHRFTDDVPMVIPEINADHIKIIDAQRKRLGTKRGFVAVKSNCSLQ
                     SYVPAIHPLKALGVDKVLACTYQAISGAGKTFKTWPEMVDNVIPYIGGEEEKSEQEPL
                     KIWGKIEGDKIVKTDDISITSQCIRVPVSDGHTAAVFMSFKDGVKKPTVEEIIDIWSN
                     YSGRAQELDLPSAPKHFLHYFTEPDRPQIKSERNLENGMAVSVGRLRPDTQYDYKFVC
                     MSHNTLRGAAGGAVLLAELLCAEGYMD"
     gene            8146..>8553
                     /locus_tag="PLS64_05275"
     CDS             8146..>8553
                     /locus_tag="PLS64_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRL43273.1"
                     /translation="MKTSDFYYDLPKELIAQTPLEPRDSSRLLVLDREKQTLEHKHFY
                     DIIDYLNEGDLLVANDSRVLPARIYGIKDETGAKVEFLLLKQVANNRWETLCKPGKKA
                     KVGTKFSFGNGILRATVVDVKDDGNRIVDFDCEE"
BASE COUNT         2356 a         1675 c         1783 g         2739 t
ORIGIN      
        1 ataacaaaat gcgaaacttg tattattctg tattgaaatt atacttgata ttgagagcgt
       61 aatttaatat aattataggg taaaattaat ttcaaaggtt gttttgggtg aaaaaagtta
      121 ttacaaatat ttatgaatat ttcagacagt gtgacgcagt gttgtggctg ttgaccttga
      181 ccgctgttgc gtacagtttt cttttgattg cgagtatgca acggtcgggc gaatacagtt
      241 atctaagaac tcagattatc gctgtgtctg tcggtcttgc ggcatcggtg tttatatcaa
      301 ttgccgatta cagatttctt atacgaaaat ggtacattgc cgcaatagtg ggaattgttc
      361 ttgccggact tgtgtttgtg ttcggagttc aggttagcgg aacagatgat acggcatgga
      421 ttaatcttcc gggcggattt tcaattcagc cgtctgagtt tataaaaatc tgttttatca
      481 taactttttc aaagcatttg tcgtggctga gagaaaagaa aatgattgaa aagttttcgg
      541 gagtactttt ccttgcactt catgcaatga taccaatggc aattattcat attcagggtg
      601 atgacggtac agttcttata tttgcgctta tgtttttggt tatgacattt gcggcaggtg
      661 ttcaactgag atattttgtc attttcggag gattgcttgt gtgtgcaatt ccgattatat
      721 ggaatgtgtt tatgaatgaa gaacatcgca acagggttct tgctttgttt gaccttgacg
      781 ggaatgcact gacaaattac ggttggcaac agtatcaggg taaggtttca attgcaagcg
      841 gtgaagcttt cggaagcgga ctttttaacg gacagcgtgt tgaacattca atcgtccccg
      901 aacaggaaaa cgattttatc tttaccgttg taggagagga actcggattt gtcggatgca
      961 tggcgctttt gctgattttg ttttttatta tattaaaagt gattttgaat gcaaaaaatt
     1021 caagtgaatt tgaaggcaag tatatctgct acggtgtgtt ttcaatgata gcgtcgcaaa
     1081 caattgtaaa tataggaatg gtgcttggat ttcttcccgt agtcggaata acccttcccc
     1141 tgttcagttc gggaggtact tcggctttga gtacgctcat tagtcttggt cttgtacaga
     1201 gcgtgagata tcacaatact gacgatatga acaaagcttt tgtaaggcga ggcagttcaa
     1261 atcgaatcag aatgtaattt aatacaaata aactaaaccg cagacatcaa aatgcctgcg
     1321 gtttttatgg gttatttttc gctgattgtg atatttgcct tgtagcttcc gcttacccaa
     1381 caggtgtcag taccgctgag tttgaaagta acaggcatct gaaccgagcc tactgttccg
     1441 ctctgatctg aggtgttaat tacgccttca atatctgagc ttttaaggct tttaagttcc
     1501 ttttcactgc cgattacggt aacattcata cttgtctgtg ttacatcggc tttgtatttt
     1561 gaagaaagac cgcttacttt aaatgaatca acggtaaagg ttttcttgga cagaccgctg
     1621 aggtcaagta caagctttgc ggaggttgag ttactgatat ttttgcagtc actcgggatg
     1681 tttattccct gcgtatcgta ctcataacgc ttgtttgaaa gtgttgcaaa atcgattgac
     1741 tcaagcttta cgctgtcagt cttgtcaagc acttcttttg gacctgcaag cagaatcttt
     1801 gaaggctcaa ccgtaagcat atcatcgttt aaatcaagac ctttgggttt gttaataaac
     1861 tcaggaatta catttaccgt cttttcgggg agaacggaaa ttgtagcctc aacatccgta
     1921 aactccattg taagaagctc ggtggagatt ttatttcctg ttttatcata aagcacaata
     1981 tttgcggtcg ttgaggtcgg ctcatttagt atactgtcaa gttcggctga tgccgaaacc
     2041 tttgcgattt tggcaatctc tgtttgaggt cctgagattg tgatattctt tgatgcaaga
     2101 gaagttgacg catagtaccc gtcggcaacc ttgtaaacaa cattttcctg aatcgggaaa
     2161 cttgtttcac gctgataatc aacaattaca ttgacatttg aaggcgtaac ggttgagata
     2221 atctgaaagt tggcactcgg gttggctttt acggctgaaa gcgaaagctg ataagttcct
     2281 gccgagtcaa ttccgtttgt agcacccgtg acagtaatat ccttcgttcc gatagaaccg
     2341 agaacggctc tgctgcctgt tacggttacc gtggcagtct gctccgtatc acggaatacc
     2401 tgcaacccgt tgttatcaag ctggtcaggc aattcaatct gaatgggaat attgcttatt
     2461 gttacggttg tatcattcgt agtacccata ctcatataaa cccatgttat tgttgagatt
     2521 acaagggcga gtataatcag aaatttatta ttctgaaaca gtttgttgag agagagtttt
     2581 ttctttttca tttttcagcc ttctctcttt ttgaactttt cgggttcttc tgaaaaaact
     2641 ttttcggcgg ttcatcatca tcatagccac gatcatagat gagaaactgt tcaagctttt
     2701 tgattagttc ttctcttgta aactcacgga gcaaaatacc gtttaccgca agagaaatta
     2761 cacctgtttc ttcacttact ataaggacaa cagcgtcgct ttgttcactg ataccgagtc
     2821 ctgctctgtg tcgtgtaccg agattgatat ctacattagg atttgatgtg agcggtaaaa
     2881 tacatcctgc tgcaaaaagt tttccgtcac ggataatgct tgcaccgtca tgcaatggtg
     2941 ctttgttaaa aaatatattg ccgaacagag ctactgatgt ttcggcatca ataattgtgc
     3001 ctgtagcggc tatatcggaa agcatggttt ctcgttcaaa cacaagcaaa gcacctgttt
     3061 ttgaacgaga gaaaagaact gctgtgtcag ccgcatctac gattgacttt tctacagcct
     3121 ttttccattc atcacctttg tggtgcttag taaatatttt tatatatttt ttataggtgt
     3181 tgttgcgtcc catctgttca agagcctttc tgatttccgg ctgaaagatg atagcaataa
     3241 caacaactgc tgcctcgaaa aatgttcgca acagggatga aagcataaca agaccaaaca
     3301 gagacgaaag aaaatacact atcaggagca taattacacc tttgagaagc tgaactgctc
     3361 ttgtttcctg aataagcttg aacagtccga aaatcaacag tgcaattgca agaatatcga
     3421 caatatctct tagctgtata gtcttgataa tggaaagaat gctgttaaat atttccaatc
     3481 tgctttcccc ttcctttatt tttaaattcc ttgttattat tctaacatac ttttgttatt
     3541 aattgcaacc tttttaaatt gtttttttct gtttgttgtt cttagaaata cgacttacgg
     3601 catttgcaaa atatacaaca tcattttttg ttgtaaatgc cgatacaaca gctcttacgg
     3661 ttcctgtatc ctgagtgccg aagcttttgt gtgcaagggg cgcacaatgg agtcctgctc
     3721 ttacggcaat gttgtagctg tcattaagaa tttttgcaac tgtttcactt tcggtatttt
     3781 tattaaagga aattacagga acgctgtgac ttttctcagg catttttgtg tataatatta
     3841 cattttccgc tttttcaagc cttttgtaga gcattcgtgc aagacctaac tcggaattat
     3901 atattctgtc tgtttttttg ttcaggacaa actttattcc tgcattaagc cctgctattc
     3961 ccggaagatt cggcgttccg ctttcgtact tatcggggag aatttcaggc tgattgaggt
     4021 ttgcggaatc acttcccgtt cctccctgaa taagactgtc cggagttgtt tcgctgttta
     4081 taataagcaa gcctgttccc atcggtccgt aaagtccttt gtgacctgcg gtgcagagaa
     4141 aatcaatgca actgtcagaa aggttgatat ctgctgttcc tgcactttgt gcggcatcta
     4201 cacaaaaaag gatgccgttt aatctgcaaa gagcgcctat tctttctatc ggaagtttga
     4261 tgccgaatac atttgaagca tgggtacaga ttacaagttt tgtgttagat ttgaattgtt
     4321 tcctgaaatt ctcgattgtt tgctcatcat cataagggac acattcggca acagagtatt
     4381 caatcttatt tttcttgagt gtttcaagcg gtctgacgac agcattgtgc tcaagtgatg
     4441 agattacggc atggtcgccc tgttttaata tgcctttaat aactgtgttc aaagccgttg
     4501 tgcagttaag cgtgaatatt atattttcgg gattttccga gccaaaaagt tttgcggcat
     4561 tatctcggca ggaatacatt atttcagatg atttcaccga cattttgtga ccgcttcttc
     4621 caggatttgc tccgtagtcg gacatagctt cgttaatcgc ttttcttacg gataagggtt
     4681 tcggaaaggt ggttgcaccg ttgtcaaaat atatcataag gtatcaacag cggctgttcc
     4741 tttgacagca ataccgttac tcctgataat ttccaatgcc ctttcaaaat ttcccgggac
     4801 ataaaggctg tatccgcatg attttcgatt gaggtgtata ggagttctta caagccctga
     4861 tttaatattg ttttttaaaa gtaggttacg gctcttcatg gcaagagtta ccgagccgaa
     4921 tattattaaa ttattttcca taacttccac cctttgttaa atctttgtga catctcaaaa
     4981 caatatatca cactacatat tatgaaagta gtcaaagttt ggacataaaa gaaagcacgg
     5041 cttttatgcc gtgcttaaag gtgttatttt tcagttcatt ttttcagaaa gaaggttgcc
     5101 catgttgtac atacctgctt tctgatcttt aagaaatact gccgcattaa cagcaccgac
     5161 agcgaaaact tcttttgact gagcctgatg agagattgtt acaacctcat cgtgacctgc
     5221 aaagataact tcgtgttcgc ctacaattgt gccgcctctt acggaatgaa taccgatttc
     5281 gttctttgaa cgctttctgc ggtagctgtg tctgtcatag acatactgcg gatcttcgtc
     5341 aagctgttcc gaaattccgt ctgcaatcat aagagctgtt ccgctcggag cgtcaagctt
     5401 gagattatga tgcttttcaa cgatttcaat atcgaactgg tttccgagaa tttttgctgc
     5461 ctcttttgaa agctcaataa gaagattgat gccgagtgac atattacctg aatagaaaac
     5521 aggaattgtc tttgccgcat cttcgatttt tgcaatctga tctttagaaa agcctgttgt
     5581 gcagataaca caaggtactt tgttatcaac tgcatacgaa agcataccgt caagagctgc
     5641 aggatttgaa aaatcaatga ttacatcagg tgtttcggtt gcctcgtcaa aagtcttgaa
     5701 aacatcaaaa tcataattac tttcaccgaa tgcgtcaaca ccgaacaaaa cattgcaatc
     5761 ctcacggctc tgaacagcct gtgccacaaa gcagcccatt ttaccgttac aaccgactat
     5821 tgctgtattt accattttta ttccccttta aacttcttga attttaaacg aatcaaaggt
     5881 tgattattta accttcttgc ttaaaaggtc atacttctcc atgcaatcct tgaggtcatg
     5941 gtaaccctga acgctcatgg gtacgagcgg aagtctgcat tcgcctgctt catagccgaa
     6001 gaggttcatg gcagtcttta cggggatcgg gttaacatcg ctgaagagta tatgcataag
     6061 ctcaagatag tggaagttaa gcttctgtgc ctctgcaaag tcattgtcaa gacaaagctg
     6121 acagatgtca tgcatttctt ttgggcagat gtttgcaaat acagagataa caccgagagc
     6181 gccgagtgac atgaaaggaa cagtctggtc atcgttacct gagtagatgt caagattgtc
     6241 accgcaaagt gaacggatga gagcaacctg agagatgtcg ccgcttgcct cttttgcggc
     6301 aacgatgtta ggatgcttgc aaagctcctg ataagtcttt ggcttgatgt tacagccggt
     6361 tcttgaagga acattgtaaa gaataatcgg aatatcaacc gaatctgcaa caacgttgaa
     6421 gtgtcttaca agaccctgct gagatgtttt attgtagtaa ggagttacgc agagaagtgc
     6481 gtcaacgcct gtgcttgcgg cagattttga gagcataacg gctgttgagg tatcgttact
     6541 gcctgtacct gcaataacag gaattctgcc gtttgtcttt tctgcaacga aagaaattgt
     6601 ttcgcaatgc tctttttccg aaagagttgc agcctcacct gttgtaccgc atacaataat
     6661 tgcatcggta ccgttttgaa catgaaattc tacgagatcg ccgagaacat cgtagtttac
     6721 gcttccgtca gacttcatag gagtgataag tgcaacacct gagcctgtaa aaatgtggtt
     6781 agacataatt tacctccgta agtaatcagt ccatgtagcc ctcagcacaa aggagctctg
     6841 caagaagaac tgcaccgcct gctgcgcctc ttaatgtgtt gtgtgacata caaacgaact
     6901 tgtaatcata ttgagtatca ggtcttaatc tgccaaccga tactgccata ccgttttcaa
     6961 gatttctttc agatttaatc tgcggacggt cgggttctgt gaagtagtgg agaaaatgct
     7021 ttggcgcact cggaagatcg agttcctgtg ctctgccgct gtagtttgac caaatgtcaa
     7081 taatttcttc gacagtaggt ttctttacgc cgtccttaaa tgacataaat acagctgctg
     7141 tgtgaccgtc tgatacggga acacggatgc actgtgatgt gattgaaata tcatcggttt
     7201 taacaatctt gtcgccttca atcttacccc agattttaag cggctcctgc tctgacttct
     7261 cttcctcacc gccgatgtaa ggaataacat tatctaccat ttcaggccat gtcttaaatg
     7321 ttttgcctgc gcctgaaatt gcctgatatg tgcaggcaag aaccttgtca acgccgagtg
     7381 cctttaaagg atgaattgca ggaacatagc tctgaagtga gcaattggac ttaactgcaa
     7441 caaagcctct ttttgtgccg agacgctttc tctgagcgtc aataatctta atgtgatcgg
     7501 cgttaatttc aggaataacc ataggaacat catctgtaaa acgatgagca ctgttgtttg
     7561 atacgatagg acactctgcc tttgcatatc tgtcttcgag atcacggatt tcatccttct
     7621 tcatattaac tgcgcagaaa cagaaatcaa cctgtgatgc aacctcttca acattttcag
     7681 cgtcaacgat aaccatatcc ttgtacttct caggaagagg agcagtcata tgccatctgc
     7741 cttcaactgt ttcaccgtat gttttgcctg cacttctctt acttgcggca agaacagtaa
     7801 cctcaaacca cgggtgattt tcaagaagaa gtgtaaaacg ctgacctacc ataccggtag
     7861 cgccgataat accgacttta tactttttca taataattcc tccgaattaa acaaatttta
     7921 aaaaagcggt tgccttattc aaacaaatat tatatcatat gaagagatgt ctgaaaacac
     7981 gactgtttgt cgattatgca aactgctcat aataagataa tatatcattt taaaagggta
     8041 atgtcaatta ttttgagaat tttacaggac aaatacttgt gttttacaaa gatttttgac
     8101 agaacagctt ttaaaaagca attttagttc ttgaaaggaa ataaaatgaa gacgagtgat
     8161 ttttattacg atttgcctaa agagcttatc gcacagactc cgcttgaacc gagggacagt
     8221 tcaagattgc ttgtgcttga cagagaaaaa caaactctgg agcataaaca tttttatgac
     8281 attattgatt atcttaacga gggagatctt cttgttgcca acgattcaag agttttgcct
     8341 gcaagaattt acggtatcaa agacgaaaca ggtgcaaaag ttgaatttct gcttttgaag
     8401 caggttgcga acaaccgttg ggaaacactt tgcaagccgg gtaaaaaagc aaaagtcggc
     8461 acaaaatttt cattcggcaa cggcattttg cgtgcaactg ttgttgatgt taaggatgac
     8521 ggcaacagaa tagttgattt tgactgtgaa gag
//
LOCUS       DAOMVE010000071         8486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2150_length_8486_cov_3.676195, whole genome shotgun sequence.
ACCESSION   DAOMVE010000071 DAOMVE010000000
VERSION     DAOMVE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2150_length_8486_cov_3.676195"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..88
                     /locus_tag="PLS64_05280"
     CDS             <1..88
                     /locus_tag="PLS64_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRL43274.1"
                     /translation="DGYTVDGTPLNLSDKDKKWLGLKDTFKF"
     gene            complement(151..756)
                     /locus_tag="PLS64_05285"
     CDS             complement(151..756)
                     /locus_tag="PLS64_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HRL43275.1"
                     /translation="MTAKPPTSTYFSVKISNILPYGGPDGELGASLRYLSQRFAMPLP
                     ELKGTLTDIGTEELNHLEMIGAIVYQLTKDLTEDEIKEQGFGDYFVDHTTGIFPQAAA
                     GFPYTAASMQVKGDPITDLHESMAAEQKARTTYDNILRFCDDYDVKDPIRFLRAREVV
                     HYQRFGENLRLLTDKLNEKNFYAFNPSFDKKCFKNELKRKK"
     gene            complement(707..1909)
                     /locus_tag="PLS64_05290"
     CDS             complement(707..1909)
                     /locus_tag="PLS64_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRL43276.1"
                     /translation="MSGNHNKNHFAAAYIRVSTENQTELSPSSQLKIIRKFADEKGYL
                     IDEDFIFRDNGISGRYADKRPEFNRMISLAKQKPPLFSAVLVWKFSRFARNQEESIFY
                     KSLLRKNGIEVISVSEPVDDGPFGKLIERIIEWSDEYYSIRLSGEVRRGMTEKAERGG
                     IVSIAPFGYKVTDGRLVVDAEKARIVSKIFADFLNNKDINMICDNLNSSEIRTAKGNR
                     WDCRAVEYILQNPVYIGKIRWNPICKTGRDFHNENLIISNGTHEPIVDEVIFEQTRQI
                     LFLQGIINREHSHKQTEIRHLIQNLVKCSNCNSTLVPIKNNFLQCSAYIHGNCHSSHS
                     VRIEKIEKVVIKAINILIKNNLINDAFSIKENYPLKTQNDLLSIITKRIIFDRKASHL
                     NIFLSQNK"
     gene            2099..2911
                     /locus_tag="PLS64_05295"
     CDS             2099..2911
                     /locus_tag="PLS64_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRL43277.1"
                     /translation="MKKKKLLSLLLAGAMSASIFVGCGSSATDWDYISNKGEFVIGVT
                     YFEPMNYLDADGKLTGFETEFAEAVCEKMGVTPKFQKIDWDSKEVELNSKTIDCIWNG
                     LTIDDDRKTTMDISTPYMENKQVMVAKSDVADKIKSADDLKDKTVVAEKKSAGETVAQ
                     TDEFFSSAKYVSVDAQAKALLEVKSGTADVAVIDYVMSIGTLKSGSDYADLKVVDGKD
                     FSPEQYGIALRKDSPETLKKLNDAIQAVADDGTLDKIAEKYSLQDLLLVKKK"
     gene            3054..3704
                     /locus_tag="PLS64_05300"
     CDS             3054..3704
                     /locus_tag="PLS64_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRL43278.1"
                     /translation="MDQFFNVSGQLLEGFGENCIIFIVTLLAAIPLGLIISMGSMSKF
                     KPLKWLTKTFVWIIRGTPLMLQLFVVFYVPPLVSNGSVTFARMNAALIAFIINYAAYF
                     SEIFRGGIESVPKGQYEAGQVLGMTKGQIFFKVVLLQVVKKITAPMGNEIITLVKDTS
                     LSSVIAIPEILMRAKDFSMQGLIWPLFYSAVFFLAFCGILTLLFSFIEKKLDYYKG"
     gene            3717..4514
                     /locus_tag="PLS64_05305"
     CDS             3717..4514
                     /locus_tag="PLS64_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRL43279.1"
                     /translation="MSLLNVENIRKSYGKTEVLKDISFSLKKGEVLAIIGSSGSGKTT
                     LLRCLNFLETPGNGKISVNDKVLFDSNDSSAKSDSEIRKRRLHFGLVFQSFNLFPQYT
                     VIENIMLAPKLAAKEQIKQTGEYMGAKSYKEAHEIIKSNAKSLLERVGLSEKSESYPC
                     NLSGGQQQRVAIARALALNPDVLCFDEPTSALDPELTGEVLNVIRSLKTSDSTMIVVT
                     HEIEFARDVADKIIFMADGVIAEEGTPEQVINNPQNPRTQAFLSRFN"
     gene            4686..5075
                     /locus_tag="PLS64_05310"
     CDS             4686..5075
                     /locus_tag="PLS64_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43280.1"
                     /translation="MKRIFTISVAAVMALLMVLSLTACGNSTLGYYKLTSAEASGMSF
                     DSSTLAAVGMDKGYILLKEDGKAKLSAAGQEQELTYDDKNFTAGSESIPYTLKDGVIT
                     MEKDGTKLVFTFDANFKPESSASESSK"
     gene            complement(5193..6476)
                     /locus_tag="PLS64_05315"
     CDS             complement(5193..6476)
                     /locus_tag="PLS64_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRL43281.1"
                     /translation="MANIKLPVPVTLLTGYLGAGKTTLINHVLGNQEGYKCAVIVNDI
                     GEVNIDAELIQKAGVVTQKDTNLVPLTNGCICCTLSVDLINQITDLVKSGKFDYILIE
                     ASGICEPLPIAQSISVLEGDKENNIPELVRLDNIVTVVDALRMATEFGCGDDLLKEDV
                     DEEDVESLLIQQIEFCSTIILNKVDKVTEEQLKRVKAMIKTLQPKARIIESQFGKVEV
                     KDILDTKCFNFEETAASAGWAQIYDKDEDDEDTDRHGHHHHHHHHDEDHDEHEHHDHD
                     HDEDGLDHSHCDHEHGHCCHHDHDHEDGEEEYGIGSFVYHRIRPFNQDKLQQFVENWP
                     KSVVRAKGVLWFDDKREDVYVFEQAGVQITATEQGKWLAAFPKKQQKLYLAEYPDMAE
                     KWDEKYGDREIKLVFIGQHMDRHAIVDALDECLSD"
     gene            6694..7257
                     /locus_tag="PLS64_05320"
     CDS             6694..7257
                     /locus_tag="PLS64_05320"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRL43282.1"
                     /translation="MIVLASASPRRKELLSFITTDFKIVPADIDETVENNVPLEKRPE
                     YLAVKKALHISKNGYEKDIVIGCDTGVFVDGKMLGKPKNREDAENMLKLLSGRQHKVI
                     TGCCTVKNGVAKSFSNVTLVEFFELSDSEIEEYIATGEPFDKAGAYGIQGKGSLLVKK
                     IDGDYFNVVGLPVSELNRILKKEYSYE"
     gene            7250..8179
                     /locus_tag="PLS64_05325"
     CDS             7250..8179
                     /locus_tag="PLS64_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HRL43283.1"
                     /translation="MNDNINKTVNEILESYSKHEQTCRLSEDNIINKSVLIQVLEEIR
                     KLLFPGYFDKNRVREEYIGYIVGDRIEFIQYNLKKQIAKALKGCEKCNDLSYDEVMEK
                     SEKLVYEFLSKIPSIRDYLATDVVAAFNGDPAAYSTDEIILCYPGFFAITVYRVAHEL
                     WKLKIPMIPRVLSEYAHGLTGIDIHPGATIGKYFFIDHGTGIVIGETTEIGENVKIYQ
                     GVTLGALSTRKGQQLKGVKRHPTIRDNVTIYSGTTILGGETVIGKGATIGGNAFIVNS
                     ITEGMKVSIKNPELQFSPDLSENNQDKYWYWMI"
     gene            8229..8477
                     /locus_tag="PLS64_05330"
     CDS             8229..8477
                     /locus_tag="PLS64_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL43284.1"
                     /translation="MVKEKSPYGLQRKFYQIAEFGVDENFRRRGVATKLISFCKQEAK
                     NRGFDRLELDVWEFNEGAMKFYDFVGFKTYREYKEMNI"
BASE COUNT         2444 a         1683 c         1874 g         2485 t
ORIGIN      
        1 agatggctac acagttgacg gcactccgct aaatctttcc gataaagata aaaagtggtt
       61 gggtttgaaa gatactttta agttttaaat acaaaaatcc ccatagattt cattgcgaag
      121 tctatgggga gtctttttgt gaattattgg ttacttcttc cttttcaatt catttttaaa
      181 gcatttttta tcgaatgagg ggttgaaggc gtagaagttc ttttcgttga gtttatcggt
      241 taagaggcgg agattttcgc cgaagcgttg gtagtggaca acctcacggg cacgcagaaa
      301 gcgaatcgga tcttttacat cgtagtcatc gcagaatctg aggatgttat cgtaggttgt
      361 tctggctttt tgctcagccg ccatactttc gtgcaggtcg gttatcggat cgcctttaac
      421 ctgcattgat gcagctgtat agggaaaacc tgctgccgcc tgtgggaaaa ttccggttgt
      481 gtggtcaaca aaatagtcac cgaatccctg ttccttgatt tcatcctcgg tgaggtcttt
      541 cgtcagctga tatactattg caccgatcat ttcaagatga ttaagttcct ctgtgcctat
      601 gtcggtgaga gtgcctttga gttccggcaa gggcattgca aatcgttgac tcagatatcg
      661 taatgacgca cccaactcac cgtccggacc gccgtatggc agaatgttac ttattttgac
      721 tgagaaatat gttgaggtgg gaggctttgc ggtcaaatat tattcttttt gtaattatgg
      781 atagtaagtc gttttgcgtt tttaagggat aattttcttt aatcgagaaa gcatcattaa
      841 ttaaattgtt ctttatgaga atattgattg ctttaattac aactttttca atcttttcaa
      901 ttcttacgct gtgcgatgaa tggcagttgc cgtgaatgta ggcggagcat tggagaaaat
      961 tatttttaat cgggacgagg gttgagttgc agtttgaaca cttgacaaga ttttgaataa
     1021 ggtgtctaat ttcggtttgt ttatgggaat gttctctatt tattattcct tgcaagaata
     1081 atatttgcct tgtttgttca aaaattactt catcaacaat cggttcgtga gtgccgtttg
     1141 aaattatcaa attttcgtta tggaaatctc tgcccgtttt gcagattgga ttccacctga
     1201 ttttgccgat ataaacggga ttttgcagaa tatattcaac ggcacggcaa tcccagcggt
     1261 tgcccttggc ggttctgatt tctgacgaat tcaagttgtc acaaatcata tttatatctt
     1321 tattattcaa aaaatcggca aaaattttac tgacaatccg ggctttttca gcgtcaacca
     1381 caagccttcc gtctgtcact ttatagccaa agggcgcaat gctgacgatg ccgccacgct
     1441 cggctttttc ggtcattcct cgccttacct cacccgaaag ccgaatcgaa tagtattcgt
     1501 ccgaccattc gataatgcgt tcaatcagct tgccgaaagg accgtcatca acgggttcgg
     1561 acacgctgat gacttcaata ccgttttttc tcaacagcga tttatagaaa atgctttcct
     1621 cctgattgcg ggcgaatcgt gaaaatttcc agacaagtac ggctgaaaac aggggaggct
     1681 tttgctttgc aaggcttatc attcggttaa attcaggtcg cttgtcggca tatcttcccg
     1741 aaattccgtt gtcacgaaaa atgaaatcct catcaatcag atagcctttt tcatctgcaa
     1801 attttctgat gattttcagc tgacttgacg gtgaaagctc agtttgattt tcggttgaca
     1861 cccttatgta tgccgccgca aagtgatttt tattgtgatt tcccgacaaa ttaatcacct
     1921 ctttaaagaa taattctttt atgcaataat atgtctgcga ttggaataaa attcttcggc
     1981 aaatttctcg aaaacacttt actttgctaa cgcactaatg tataatatac agagattgtt
     2041 ttgcaaggtg atttgcagaa cataggtaga gagtatatca gattacggag gaataattat
     2101 gaaaaagaaa aagttacttt cattacttct tgcaggtgca atgtcagcgt caatttttgt
     2161 aggttgcggt tcttctgcaa cagactggga ttatatctca aacaagggag agtttgttat
     2221 cggtgttaca tattttgagc cgatgaacta ccttgatgca gacggcaagc ttacaggttt
     2281 tgaaactgag tttgcagagg cagtttgcga gaaaatgggc gttactccaa agttccagaa
     2341 aattgactgg gattcaaaag aggttgagct taactcaaag acaatcgact gcatctggaa
     2401 cggccttaca attgatgacg acagaaagac aacaatggat atttcaactc cttatatgga
     2461 aaacaagcag gttatggttg caaaatctga tgttgcagac aaaatcaagt cagcagatga
     2521 cctcaaggat aagacagtag ttgcagagaa gaagtcagca ggtgaaactg ttgctcagac
     2581 agacgagttc ttctcaagtg caaagtatgt ttctgttgac gcacaggcta aggctcttct
     2641 cgaggttaag tcaggcacag cagatgttgc ggttatcgac tatgtaatgt caatcggtac
     2701 actcaagagc ggttctgact acgctgacct caaggttgtt gacggcaagg acttttcacc
     2761 tgagcagtac ggtattgcac ttcgtaagga cagccccgaa acactcaaaa agcttaacga
     2821 tgctattcag gcagttgccg atgacggcac acttgataag attgcagaga agtacagcct
     2881 tcaggatctt cttcttgtaa aaaagaaata atttaattaa aataagtttg tacatacagg
     2941 gcggactatg ttcgcccgtt gtgcttttta ttgattataa atttacaggt tcaaatttgt
     3001 aagccttttg atatattcaa aactgaaaat acttggatag agtggtatat aaaatggatc
     3061 aattttttaa tgtaagcggt cagttgcttg agggattcgg cgaaaactgt ataattttca
     3121 ttgtaaccct gcttgcggca attccgctcg gacttattat ctcaatgggt tcaatgtcaa
     3181 aattcaagcc gttaaaatgg ctgacaaaaa catttgtgtg gataatcaga ggtactccgc
     3241 tgatgctcca gcttttcgta gtgttctatg ttccgccgct tgtgtcaaac ggcagtgtga
     3301 catttgcacg aatgaacgcc gcacttatcg catttataat caattatgcc gcatattttt
     3361 ctgaaatctt ccgtggcggt atcgagtctg ttccaaaggg acagtacgag gcaggtcagg
     3421 tgctcggcat gacaaagggt cagattttct ttaaggttgt gcttttgcag gttgtaaaga
     3481 aaattactgc acccatgggc aacgaaatta ttactcttgt aaaggatact tcgctttcaa
     3541 gcgttatcgc aattccggaa attcttatga gagcaaagga cttctcaatg cagggactca
     3601 tctggccgct tttctattcg gcagtattct tcctcgcatt ctgcggaatt cttaccttgc
     3661 ttttctcatt tatcgagaaa aagcttgatt actacaaggg ttaaggggga aaggttatgt
     3721 ctttgttaaa tgttgaaaac atccgtaaaa gctacggcaa aacagaggtt ttgaaagata
     3781 tcagcttttc tcttaaaaag ggcgaggtgc ttgcgattat cggctcatcg ggttcgggca
     3841 agactacgct gttaaggtgt cttaattttc ttgaaactcc gggcaacggc aagatttccg
     3901 tcaatgacaa ggtgcttttt gacagcaatg acagctctgc aaagagcgac agcgaaatca
     3961 gaaaaagacg gcttcacttc ggacttgtgt tccagtcatt taacctgttt ccgcagtata
     4021 cggttatcga gaatattatg cttgctccaa agcttgccgc aaaagagcag ataaagcaga
     4081 caggcgaata tatgggcgca aaaagctata aagaggctca tgaaattatc aagagcaatg
     4141 caaagtcttt gcttgaaagg gtaggacttt ccgaaaagag tgagtcatat ccttgtaacc
     4201 tcagcggcgg tcagcaacag cgtgttgcaa ttgcaagagc gcttgcactc aatcccgatg
     4261 ttctctgctt cgacgagcct acctccgcac ttgatcccga actcacgggt gaggtgctta
     4321 atgttatccg cagtcttaaa acctccgaca gcacaatgat tgttgtaact cacgaaattg
     4381 agtttgcaag agatgttgcc gacaagatca tttttatggc tgacggtgta attgccgaag
     4441 aaggcactcc cgaacaggta atcaacaatc cgcaaaatcc aagaacccag gcgtttttgt
     4501 caaggttcaa ctgataactg aatattaggt gaggaaagca ctcgaaaggg tgctttttta
     4561 catattttta caaataacct tgaaaagttt atgccattgt gctataataa tcgtgtcatt
     4621 cactgacaaa ggcacaaagc ctgaaaaaat gagaaatccc gatagggaaa acggaggaaa
     4681 aacaaatgaa aagaattttc acaatcagcg ttgcggctgt tatggcactt cttatggtgc
     4741 tttcacttac agcttgcggt aattcaacac tcggctatta caagcttaca agtgcagaag
     4801 caagcggtat gagctttgac agcagtactc ttgccgctgt cggtatggac aaaggctaca
     4861 ttcttcttaa agaagacggc aaggctaagc tttctgcagc cggtcaggag caggagctca
     4921 catacgatga caagaacttt acagcaggga gcgagagcat tccatacaca ctcaaagacg
     4981 gtgttattac aatggaaaaa gacggcacaa agctcgtttt cacatttgac gctaacttca
     5041 agcccgaatc ttctgcttca gaatcatcaa agtaataaaa cttaacggtg caaaaatttc
     5101 ggcagtccaa atcggactgc cgtttttgta tatccaaccc aaaaaaacaa gggcagcgga
     5161 atttcgctgc cctcaatttt tataagattt tatcagtctg aaagacactc gtcaagcgca
     5221 tcaacaattg cgtgcctgtc catatgctga ccgatgaata caagcttgat ttctctgtcg
     5281 ccgtattttt cgtcccactt ttcagccata tccggatact ctgcaagata gagcttctgc
     5341 tgtttctttg ggaatgcggc aagccacttg ccctgttccg tagcagtaat ctgaacgcct
     5401 gcctgctcga atacataaac atcttctctc ttgtcatcaa accagagaac accctttgca
     5461 cggactacgc tctttggcca gttctcaaca aactgctgga gcttgtcctg attgaaagga
     5521 cgaatgcggt ggtaaacaaa tgaaccgatg ccgtattctt cttcgccgtc ctcatgatcg
     5581 tggtcgtggt gacagcaatg accgtgttcg tggtcgcaat gagagtggtc aagaccgtcc
     5641 tcatcatggt catgatcgtg atgctcatgt tcgtcatggt cttcatcgtg gtggtgatgg
     5701 tggtgatgat gaccgtgacg gtctgtatcc tcgtcatctt catccttatc ataaatctga
     5761 gcccaacctg cggatgcggc tgtttcttca aaattaaagc actttgtatc gagaatatcc
     5821 ttaacttcaa ccttgccgaa ctgcgattca ataattcgtg cctttggctg aagtgtctta
     5881 atcatagcct taactctctt gagctgttcc tctgttacct tgtcaacctt gttgaggatg
     5941 attgttgaac agaactcaat ctgctggata agaagacttt caacatcctc ttcgtcaaca
     6001 tcttccttga gaagatcatc gccgcaaccg aattccgttg ccatacggag tgcgtcaaca
     6061 actgtaacaa tgttgtcaag acggacaagc tcgggaatat tgttttcctt atcgccctca
     6121 agaactgaga ttgactgtgc aatcggaagg ggttcgcaaa taccgctcgc ctcaatgaga
     6181 atgtagtcaa acttgcctga ctttacaagg tctgtaatct ggttgataag gtcaacgctc
     6241 aaagtacagc agatacagcc gtttgtaagc ggaacaaggt ttgtatcttt ctgagtaaca
     6301 acgcctgcct tttgaataag ctctgcgtca atgtttactt caccgatatc gtttacgata
     6361 acggcacact tgtagccctc ctgattgccg aggacatggt tgataagagt agttttgcct
     6421 gcaccgagat aacctgtaag aagcgttacg ggaacaggaa gcttaatgtt agccatatat
     6481 ttcatttcct ttcgttgaat tgtgcttatt aatgcggatt accgctgtcc ttaaatataa
     6541 cactttgcaa aattattgtc aatacaaagt acggagtgcg gtcaatccga atagctgatt
     6601 attgctgtac attttgtgtt ttttgtataa tcacctttta tttatttcgg ttttatgtta
     6661 caatagtcaa aagtaatttt acggagatag attatgattg tacttgcatc tgcttcacca
     6721 cgaagaaaag agcttttaag ttttataaca accgatttta aaattgttcc cgcagacatt
     6781 gacgaaacgg ttgagaacaa tgttccgctt gaaaagcgtc ccgaatatct tgccgtcaaa
     6841 aaggcattgc atatttcaaa aaacggctat gaaaaagata ttgtaatcgg ttgtgacacg
     6901 ggagtttttg ttgacggcaa aatgctcggc aaacctaaga acagggaaga tgccgagaat
     6961 atgttaaaac tgctttcggg cagacagcac aaggttatca cgggttgttg taccgtcaaa
     7021 aacggtgttg caaaatcatt ttccaatgta acgcttgttg aattttttga actttccgat
     7081 tcggaaatag aggagtacat tgcaacgggc gagccttttg acaaggcagg tgcatacgga
     7141 atacagggca aaggttctct gcttgtaaag aagattgacg gcgactattt taatgttgtc
     7201 ggacttcccg taagtgaatt aaacaggatt ttgaaaaagg agtattcata tgaatgataa
     7261 tataaataaa acagtcaatg aaattctcga aagctattca aagcatgaac agacttgcag
     7321 actttcggag gataacatta tcaacaaatc tgtgcttatt caggtgcttg aagaaatccg
     7381 caagctgttg tttccgggct actttgacaa gaacagagta agagaagaat acatcggcta
     7441 tattgtaggc gacagaatag aatttattca gtacaacctt aaaaagcaga ttgcaaaggc
     7501 tttaaagggt tgcgaaaagt gcaacgacct ttcatatgac gaggttatgg aaaagtcaga
     7561 aaagctggtt tatgagtttc tctcaaagat tccgtcaatc cgtgattacc ttgcaacaga
     7621 tgttgttgcg gcctttaacg gcgatcctgc cgcatacagt acggatgaaa tcattctttg
     7681 ctatcccgga ttttttgcaa tcacggttta cagagtggca catgaattgt ggaaacttaa
     7741 aatcccgatg attccgagag tgctttcgga gtacgcacac ggacttacgg gaattgatat
     7801 tcaccccggt gcgacaatcg gcaagtattt cttcatcgac cacggcacag gcattgtaat
     7861 cggtgaaact accgagatag gcgagaatgt aaaaatctat cagggtgtaa ccctcggtgc
     7921 gttgtcaaca agaaaaggtc agcagcttaa aggtgttaaa agacatccta ctatccgtga
     7981 taatgttaca atctattcgg gtacaactat cctcggcggt gaaaccgtaa tcggcaaggg
     8041 tgcaacaatc ggcggtaatg cctttatcgt gaactcaatt actgagggta tgaaagtaag
     8101 cattaaaaac cccgaactcc agttcagtcc cgacctttcg gaaaacaatc aggacaagta
     8161 ttggtattgg atgatttaag ctgacgcttt aatggaaaat tgaatgtgga tttgcaactg
     8221 ttgaatacat agtcaaggag aaatctccgt acggtttgca gagaaaattc tatcagattg
     8281 ctgaattcgg agttgatgaa aacttccgca gaaggggtgt tgcaacaaag ctgataagct
     8341 tctgcaaaca agaggctaag aacagaggct ttgacaggct ggaacttgat gtgtgggaat
     8401 tcaacgaggg tgcaatgaag ttttacgatt tcgtaggatt caaaacctac agagaataca
     8461 aggaaatgaa tatttaaacc gaaaaa
//
LOCUS       DAOMVE010000072         8428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2172_length_8428_cov_3.689239, whole genome shotgun sequence.
ACCESSION   DAOMVE010000072 DAOMVE010000000
VERSION     DAOMVE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2172_length_8428_cov_3.689239"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1260)
                     /gene="mnmA"
                     /locus_tag="PLS64_05335"
     CDS             complement(187..1260)
                     /gene="mnmA"
                     /locus_tag="PLS64_05335"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRL43285.1"
                     /translation="MTNKALIAMSGGVDSSVAAFLTKDMGYDATGITLKLFDNEDIGE
                     KREKTCCSLDDIDDARRVCLKLGIPYYVYNFKDSFKENVIDRFIRAYENGTTPNPCID
                     CNRFIKFEKLIRRAEELDFDCVVTGHYSTIEYDKGADRYLLKKSVDSTKDQSYVLYCL
                     TQRQLAKTLLPLGTYTKEKVREIANELGLINARKHDSQDICFVPDGDYAKFIEQYTGR
                     TYPNGNFVDTKGHILGEHKGIIRYTVGQRKGLGLALPHPMYVKEKNLDTNEVILCDNN
                     ELFSKELYATDINLITCDKIDKPIKVKARVRYSQPEKDAIVEQIDDNTLHIVFDEPQR
                     AISKGQAAVLYDGEYVVGGGTII"
     gene            complement(1300..2214)
                     /gene="nadA"
                     /locus_tag="PLS64_05340"
     CDS             complement(1300..2214)
                     /gene="nadA"
                     /locus_tag="PLS64_05340"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRL43286.1"
                     /translation="MNISSIQEQILKLKKEKDVCILAHSYQAHEILEIADYTGDSYKL
                     SVDASKAENKNILMCGVRFMAETCKILSPEKTVYLAQPVAGCSMADQMDKQLISQVKK
                     MYPDYTVVAYINTTAELKTICDVCVTSSSAVKIVNNLDNKNILFIPDCNLGDYVAKQC
                     PDKNIKLLNGGCPIHACVNVEDVKKAKELHPNALVLVHPECTPEVVAMADYVGSTSGI
                     MNYAKESDAKEFIIGTEISIAEHLQYECPDKNFYGLTLKLICPNMKSTTLVDVYNCLN
                     GNGGEEIKLDDQTITDARKCIDEMIRLG"
     gene            2477..2977
                     /locus_tag="PLS64_05345"
     CDS             2477..2977
                     /locus_tag="PLS64_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43287.1"
                     /translation="MKKTIAVLFAVMMMLLSAVPAFAAVSPQPNTFKYKVEVIPTEGG
                     NGNYKFTTGIDENGEQHVYIEPIPYDGYTFDHWEISGGPFRTDSKYTDANMNIIISGD
                     LVCTPYYRKASSGGTTGPVATATAKKDDSTTSPKTGASDVVPYAILVLSLAACGAAVF
                     KLVKSK"
     gene            3171..3968
                     /gene="srtB"
                     /locus_tag="PLS64_05350"
     CDS             3171..3968
                     /gene="srtB"
                     /locus_tag="PLS64_05350"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HRL43288.1"
                     /translation="MSRKRNKFLIALIILLVAAIIGTLAYIVVKNLNENNERKSLDNI
                     AGSYASGIENGTTSATEVTSPSINVKKVENPIDFKSLQQQNSDIYSWIYIPNTNVNYP
                     VLQSHLDDNLYLDHDIYKNYSFSGSIYSQMCNKRDYSDRVTVLYGHNMANGSMFATLH
                     RFYDADFFNKNRYIYVYTPDRKLTYEIVSAFEYDNRHIMNSFDFSDDNVFSDYLNMIK
                     NPHSVSVNKRNTELTTDDKILVLSTCLNSGDGRYLVQGVLKKDEPTK"
     gene            3952..5274
                     /locus_tag="PLS64_05355"
     CDS             3952..5274
                     /locus_tag="PLS64_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRL43289.1"
                     /translation="MNRQNESSAEATTENIENKLDPNTPEILESKREKNSKEHVSNPS
                     RLDIEGYIFKDSKLIKKKKKVYTDVYGVDSDSIVVKSNTTNHYKKHRSKSHHHHSKHR
                     KMKTWKKVLLSIGCTLLGLIILTVGTVAYLIYQGGNELFNTDIHVTAPQGIETEENGK
                     YVVYNGETYQFNEKVTNVLCIGVDKRNLDENNNSKVKASGGQADVLMLVSIDTSNGKI
                     TLFNISRDSMVDVTMYSAGGAYAGTEKQQICLSYSYGDGKESSCENTVNSVQRLFYNI
                     PINTYFSLDLDGISALNDSVGGVDVVSPETIGEFVEGESYHLVGQQAESFVRTRDTSK
                     TDSNNMRMQRQKVYIQSFMNTVLQQTKEDLTTPIDLFNASAPYSCTNLNPSKVSYLAQ
                     QAVLGGMNGIDMVSVPGQVAMGSKYAEFNVSEAEFYQLFLSIYYTKIE"
     gene            complement(5639..6820)
                     /locus_tag="PLS64_05360"
     CDS             complement(5639..6820)
                     /locus_tag="PLS64_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRL43290.1"
                     /translation="MPNYEKNSQKSNFVDISSNKQVKKLYNKKGRLKRILCLVFSIIF
                     LLGGGGMLYYYSFLDTLNFKELDDTNNNTAATNSSVAPLESDATNLSLSEGELLQNSK
                     ELNVMLFGEDNSKGDKHGRSDTMIMMTIDNNHKKLKLTSFQRDTYVYIPGYGYDKLNA
                     SYNYGGAKLSIQTIEANFGIKVDRYAVVDFDSFKKIIDTLGGIDMEVTQDEIDYINYQ
                     MYKNNQADTRTTITDAPGTVHLNGQEALWYARNRGLKKGEDGNEIGLDGDDWDRTSRQ
                     RKLLETLFTSMKSADLGQIVSIVSSVGPLVTTNLKKDEITALVSHALTYLSYDVEQYY
                     VPEEGLWYYDDKTETWNGAITSTIKISDLEEQRKKFASFVFEELFTGGTSSKETTSAS
                     N"
     gene            complement(6981..7568)
                     /gene="yfbR"
                     /locus_tag="PLS64_05365"
     CDS             complement(6981..7568)
                     /gene="yfbR"
                     /locus_tag="PLS64_05365"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HRL43291.1"
                     /translation="MNISHFYAMLSRMRYINRWGLMNNTRCENLSEHSLDVAVIAHCL
                     VLIHNKRFGGNLNAERAALLALFHDATEIITGDMPTPVKYFNPEIKNAYKEIETTAGN
                     RLVSMLPDDFKDDMDKIIKMNGAGDDELKKYVKAADKFSALIKCIEEIKMGNSEFSKA
                     KEATEKSIHNMNMPENEVFEKEFLPSFYLSLDEQD"
     gene            7823..>8428
                     /locus_tag="PLS64_05370"
     CDS             7823..>8428
                     /locus_tag="PLS64_05370"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRL43292.1"
                     /translation="MPSRILQKYPTQKLFGLRVFLISIITATALFLPFIIMGGGVFYY
                     YGDFNVQEIPFYQLVHDAVRNGDTGWMHNVDLGTDMLSSFSFYLLGSPFFWLTIPFPS
                     EAVPYLIGPLLILKFGCASLSAYLYLKRYVADRNFALVGGLLYAFSGFSIYNVFFFHF
                     HEPMIMFPLLLAALDAFIYDGKRMVFTLAVFSACVVNYYFFV"
BASE COUNT         2370 a         1622 c         1751 g         2685 t
ORIGIN      
        1 ggggcgttca tcggacgccc gccttgatga aattttttgt ttaataaaat gttttgagga
       61 gatgaaaata gtgtgtaatc tgtagcggcg aaccgtgttt tcctgcctga aatataatcg
      121 caatgctatc aatctaaaaa gagagcgtaa aatcacgctc tctaaaatca tcataattat
      181 aaaactttat ataatcgttc cgccgccaac tacatattca ccgtcataga ggactgctgc
      241 ctgacctttt gagattgctc tttgaggttc gtcaaagaca atgtgcaggg tgttatcgtc
      301 aatctgttca acgattgcgt ccttttcggg ctggctgtaa cgaactctcg ccttgacttt
      361 aatcggcttg tcgattttgt cacaggtgat gaggtttatg tccgttgcat aaagctcctt
      421 tgagaaaagc tcgttgttat cgcagagaat aacctcgttg gtgtcaagat tcttttcctt
      481 aacatacata gggtggggga gtgcaagtcc aagacctttt cgctgaccta cggtgtaacg
      541 gataattccc ttgtgttcac cgagaatatg acctttggta tcaacaaaat taccgttagg
      601 ataagttctg cccgtgtact gttcaatgaa ttttgcatag tccccgtcgg gaacaaagca
      661 aatgtcctga ctgtcgtgtt ttcgtgcgtt tatcaaacca agctcatttg caatttcacg
      721 caccttttcc tttgtatatg tgccaagagg aagaagtgtt tttgcaagct gtctttgtgt
      781 aagacagtac agcacatagc tttggtcttt tgtgctgtca accgattttt tcagcaaata
      841 gcggtctgca cccttgtcat actcaattgt tgagtaatgc cccgtgacaa cgcagtcaaa
      901 gtcaagctct tctgcacggc gaatgagctt ttcaaatttg ataaagcggt tgcagtcaat
      961 gcagggattc ggtgttgtac cgttttcata ggctctgata aagcggtcga ttacattttc
     1021 cttaaagctg tccttgaagt tatagacata gtagggaatg ccgagtttga ggcaaactct
     1081 ccttgcatca tcaatgtcat caagagaaca acaggttttt tctcgctttt caccgatatc
     1141 ttcgttatca aagagcttga gggtaatgcc cgttgcgtca tatcccatat ccttggtaag
     1201 aaatgcggca accgagctgt caactcctcc gctcattgca atcaatgctt tgttagtcat
     1261 ttattcctcc cgaacagatt tgcgttcata agtaatttat cagccgagtc tgatcatttc
     1321 gtcaatgcac tttcttgcgt ctgtgatagt ctgatcgtca agcttgattt cttctccgcc
     1381 gttgccgtta aggcagttgt aaacatcaac aagtgttgtt gatttcatat tcgggcagat
     1441 gagctttaat gtaagaccgt agaaattctt gtccggacat tcatactgca aatgttcggc
     1501 aatgctgatt tctgtaccga taatgaattc ctttgcgtcg gattcctttg cataattcat
     1561 tatgcccgat gttgagccga cataatcagc catagcaaca acttcgggag tgcattcggg
     1621 atgaacaaga acgagtgcgt tcggatgaag ctccttagcc ttcttaacat cctcaacatt
     1681 tacgcaggca tgaatcggac aacctccgtt gagaagcttg atgttcttgt cgggacactg
     1741 ctttgcaaca tagtcgccga ggttgcagtc agggataaag agaatgttct tattgtcaag
     1801 attgtttacg attttaacag ccgatgagct tgttacgcat acatcgcaaa ttgttttgag
     1861 ttctgccgta gtattgatgt atgcaacaac tgtgtaatcg ggatacattt tcttaacctg
     1921 agaaataagc tgtttgtcca tctggtctgc catactgcag ccggcaacag gctgtgcaag
     1981 gtaaacagtt ttttcaggtg aaagaatttt gcaggtttca gccataaatc ttacgccgca
     2041 cataagaatg ttcttgtttt cggctttgct tgcgtcaaca ctcagcttgt agctgtcgcc
     2101 tgtgtaatct gcaatttcaa gaatttcatg cgcctgatag ctgtgggcaa gaatacagac
     2161 atccttttcc tttttcaact tgagaatctg ttcctgtatt gatgaaatat tcataatagt
     2221 tttcctttca aggtaattaa aattactccc ccttattata cattttcagc agtttatatg
     2281 caatagcttt cggtgtaaaa aagctgtatt ttttgcgttt ttaacaagaa acagcccata
     2341 tttttgcttg tatttaaaaa aatatttttg ttcctttaga ctttacacat aaatgaatat
     2401 gtgctataat taggtataat ttttgaatag tatcatatcc caaagttaga atgggttaaa
     2461 ttagggggat tatgtgatga aaaagacaat tgctgtgtta tttgcagtta tgatgatgtt
     2521 attgtcggca gttcccgcat ttgctgccgt aagtcctcag ccgaatacat tcaaatataa
     2581 ggttgaagtt attccgacag agggcggtaa cggtaattat aagttcacaa caggtattga
     2641 cgaaaacggc gaacagcatg tttacattga gccgattcct tatgacggtt atacttttga
     2701 ccactgggaa atttcgggcg gtcctttcag aaccgacagc aagtatacag atgctaatat
     2761 gaatattatt atttcaggtg accttgtttg tactccttat taccgtaagg caagcagcgg
     2821 cggcacaaca ggtcctgttg ctacagctac ggcaaagaaa gatgattcaa caacttcacc
     2881 aaagacaggc gcaagcgatg ttgtaccgta tgcaatttta gttctttctc ttgcggcttg
     2941 cggtgcggct gtatttaagc ttgtaaagag caaataatcc aaccttaact aaaaatcaag
     3001 agacttctca taaggaagtc tcttttttat tcattagatg tttgaataat caggttttgt
     3061 attgcagatg cagtagtttg taaattgtaa taaaatttta ctaacaggta atgattaatt
     3121 gacatactga cacaaaaatg atacaatata accaattggg agtttttgat atgagcagaa
     3181 aaagaaacaa atttcttata gctttgatta ttttgcttgt ggctgcaatt atcggcacac
     3241 ttgcgtacat agtcgttaaa aacttaaatg aaaataacga acgaaaaagt cttgataaca
     3301 tcgcaggctc gtatgcgtca ggcattgaaa acggtacaac ctctgcaaca gaagtgactt
     3361 caccgtcaat caatgtgaaa aaggtcgaaa atccgattga tttcaagtcg cttcaacagc
     3421 agaacagcga catttattcg tggatttaca ttccgaatac aaatgtaaat tatcctgttt
     3481 tgcaaagtca tcttgacgat aacttatatc ttgaccacga tatttataag aattacagct
     3541 tttcgggcag tatctattcg cagatgtgca acaagcgtga ttattccgac agagtgactg
     3601 tgctttacgg tcataatatg gcaaacggct caatgtttgc aacgctccat cgcttttatg
     3661 atgccgattt ctttaacaag aacaggtaca tctatgttta cactcccgac agaaagctta
     3721 catacgaaat tgtgtcagct tttgaatatg ataaccgaca cattatgaat tcgtttgatt
     3781 tttccgatga taatgtattt tcggattatc tcaatatgat taaaaatcca cattctgttt
     3841 ctgtgaacaa gagaaatacg gagcttacta ccgatgataa aattcttgtt ttaagcacat
     3901 gtttaaacag cggtgacgga agatatcttg tgcagggagt gctgaagaaa gatgaaccga
     3961 caaaatgaaa gttcggctga ggcaacaact gaaaatatag aaaacaagct tgatccgaat
     4021 acaccggaaa ttcttgaatc gaaaagagaa aagaacagta aggagcatgt aagcaatcct
     4081 tcaaggttgg atatagaagg ttatattttc aaggacagca agttaataaa gaaaaagaaa
     4141 aaagtatata cagatgtgta cggagttgat tccgacagca ttgtcgtgaa gagtaacacc
     4201 acaaatcact acaaaaagca tagatcaaag tcgcatcatc accattcaaa gcatcgaaag
     4261 atgaaaacct ggaaaaaggt gcttttgtca atcggctgta cattacttgg tttaattata
     4321 ctcaccgtag gcacggttgc gtatttgatt tatcagggcg gaaacgagct gtttaatacg
     4381 gatattcatg ttaccgcccc acagggcatt gaaaccgagg aaaacggtaa gtatgtcgta
     4441 tacaacggtg aaacatatca gttcaacgaa aaggtgacga atgttctgtg tatcggtgtt
     4501 gacaagcgca accttgacga aaataataac agcaaagtca aggcgagcgg cggtcaggcg
     4561 gatgtgctta tgcttgtgtc aatcgacact tcaaacggta aaataacgct cttcaacatt
     4621 tcgagagatt cgatggttga tgtcacaatg tattcagcag gcggtgcata tgccggcacg
     4681 gaaaaacagc agatttgtct ttcgtattca tacggtgacg gtaaggaatc aagctgtgaa
     4741 aatacggtaa attcagttca gaggctgttc tataacattc cgattaatac atacttttcg
     4801 cttgaccttg acggcatttc cgctctcaac gacagcgttg gcggtgttga tgttgtgtca
     4861 cccgaaacaa tcggggagtt tgttgaggga gaaagctatc accttgtcgg acagcaggct
     4921 gaaagctttg tcagaacccg tgatacaagc aagaccgatt caaacaatat gcgtatgcaa
     4981 agacagaagg tttacattca gtcttttatg aatacggttc ttcagcagac aaaggaagat
     5041 ttaaccacgc caatcgattt gttcaatgcg tccgctccgt attcatgtac aaatcttaac
     5101 ccgtcaaagg tgtcatatct tgctcagcag gctgtgcttg gcggtatgaa cggaattgat
     5161 atggtttcag tccccggtca ggttgcaatg ggttcaaagt acgcagaatt caatgtcagc
     5221 gaggcagaat tttatcagct gtttttgagt atttattata cgaaaataga ataataatca
     5281 aacaaataaa atgcatcaga ttccaagtaa ggttctgatg catttttgtt ttatgtgcaa
     5341 ggcggttgtt atattgcagc ggaattttca tgatatcaaa acatatttat tgaacaaaaa
     5401 aattcattag agcaggcgga taatatccgc ccttacggct cggtttgtag aaaaaactgc
     5461 aaatgctacg ctcgggcata atatgttttg tatttaaaca aactgtcttc acgaccgcaa
     5521 ttttccattt tacattttca atttttcatt taattaacct gtcttcacga atagggtacg
     5581 gcggctcgcc gtaaaaaaca gacagccgat aattggctgt ctgcattttg ttcaatattt
     5641 aatttgatgc ggaggttgtt tcttttgaag aagtgccgcc tgtgaacaat tcttcaaaaa
     5701 cgaagcttgc aaacttcttt ctctgttctt caaggtctga aattttgatt gttgatgtga
     5761 ttgcaccgtt ccatgtttcg gttttatcat catagtacca aagaccttct tcaggaacat
     5821 aatactgctc tacatcatag gaaagatatg tgagagcgtg agaaacaagc gctgtgattt
     5881 cgtccttctt gaggtttgtt gttacaagag gaccgacaga gcttacaatt gaaacaatct
     5941 gaccgaggtc tgcacttttc atacttgtga acaaagtttc aagaagcttt ctctgtcttg
     6001 aggttctgtc ccagtcgtca ccgtcaagac cgatttcgtt gccgtcctcg ccctttttaa
     6061 gacctctgtt tcgagcatac caaagagcct cctgaccgtt gagatgaaca gtaccgggtg
     6121 cgtctgtgat agttgttctt gtgtcagcct gattgttctt gtacatctga tagttgatgt
     6181 agtcaatttc gtcctgagta acttccatat caataccgcc gagggtgtcg ataatcttct
     6241 taaagctgtc aaagtcaaca accgcataac ggtcaacctt gatgccgaag tttgcctcaa
     6301 ttgtctgaat tgaaagcttt gctccgccgt agttataaga tgcgttgagc ttgtcatatc
     6361 cgtaaccggg gatataaaca tatgtgtcac gctggaatga tgtcagttta agctttttgt
     6421 gattgttatc aatggtcatc atgatcattg tatcacttct gccgtgttta tcgccttttg
     6481 aattgtcttc gccaaaaagc ataacattaa gctccttgga gttctgcaga agctctccct
     6541 ctgaaagtga aaggtttgtt gcatcactct caagcggggc aaccgatgag ttggttgctg
     6601 ctgtgttgtt gtttgtgtca tcaagctctt tgaagttgag agtgtcaaga aatgaatagt
     6661 agtagagcat tccgccgccg cccaacagga agattattga aaacacaagg cagagaattc
     6721 ttttaagtct gcctttttta ttatataatt ttttgacctg cttatttgag cttatgtcaa
     6781 caaaattgct tttctgactg tttttttcgt aatttggcat attactcctc caatcgggta
     6841 aaaagcacac aatacataag tatagtttat tataccatat gagaatttct tttaatgcta
     6901 aattagaatt aaaatctgcc ttttcgtaaa gttttagcga aaaatctgac tgaaaatatt
     6961 gatttttttg tcggtaactg tcaatcctgt tcatcaagtg acaaatagaa ggacggaagg
     7021 aactcttttt caaacacctc gttttcgggc atattcatgt tgtgaattga tttttcggtt
     7081 gcctcttttg cctttgaaaa ctcgctgttg cccattttta tttcttcaat acattttatg
     7141 agtgcggaaa acttgtccgc cgcttttaca tattttttaa gttcatcgtc acctgcaccg
     7201 ttcattttga tgattttatc catatcgtcc ttaaaatcat ccggaagcat cgacacaagc
     7261 ctgttgcctg ccgttgtttc aatctccttg taggcatttt tgatttcggg gttgaagtat
     7321 tttacgggag tcggcatatc gcccgttatg atttcggttg catcgtgaaa aagtgcgagc
     7381 aatgcggctc tttcggcatt gagattaccg ccgaaacgct tgttgtgaat caaaacaagg
     7441 caatgtgcaa tcactgcaac atcaagactg tgttcgctga ggttttcaca gcgtgtgttg
     7501 ttcataagtc cccaacggtt gatgtacctc attcttgaaa gcattgcgta aaaatgtgaa
     7561 atattcatag gtagtttctc ctgttttttg ctaaaaatta gtgcaatttg tatcgcatta
     7621 tgttataatg taatctaaat gcgagtgtcc tctgttctct gcaagcaaag cagagcgttg
     7681 ctccgaattg aatatgtagg tgtcttttga ataaatgacg gtgaaggaca tttatattac
     7741 tgattatact attcataatt taaaagtaaa gaaattatcg aaaaaaagat tttttcagac
     7801 tttttgctcc gtggagggtt ttatgccgag cagaattttg caaaaatatc caacacaaaa
     7861 actattcggt ttgcgtgtgt ttcttatatc aattataact gcaacagcac tttttctacc
     7921 gtttatcata atggggggcg gtgttttcta ttattacggt gatttcaatg ttcaggaaat
     7981 tccgttttat cagcttgtgc atgacgccgt aagaaacggt gatacgggtt ggatgcacaa
     8041 tgttgacctc ggaacggata tgctctcaag cttcagcttt tatcttcttg gcagtccgtt
     8101 cttttggctg acaattccgt tcccgagtga ggctgtaccg tatcttatcg gaccgttgct
     8161 gattttaaaa ttcggctgtg cgtcactttc cgcatatctc tatttgaaaa gatatgtggc
     8221 tgacaggaat ttcgctcttg taggcggatt gctgtatgca ttttcgggat tttcaatcta
     8281 caatgtgttc tttttccact ttcatgaacc gatgataatg tttccgctgt tgcttgcggc
     8341 acttgacgca ttcatctatg acggaaaaag aatggtgttc acacttgcgg tattctccgc
     8401 ctgtgttgtg aactactact tctttgtg
//
LOCUS       DAOMVE010000073         8242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2240_length_8242_cov_3.317699, whole genome shotgun sequence.
ACCESSION   DAOMVE010000073 DAOMVE010000000
VERSION     DAOMVE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2240_length_8242_cov_3.317699"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLS64_05375"
     CDS             <1..226
                     /locus_tag="PLS64_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43293.1"
                     /translation="NGIIAWCWAYQSINSISVPIKQGTDILGIVMAKASFGMFAYLIS
                     GIAIVVFAECIVSVFIKYRTKKKQQLIHNI"
     gene            complement(319..981)
                     /locus_tag="PLS64_05380"
     CDS             complement(319..981)
                     /locus_tag="PLS64_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HRL43294.1"
                     /translation="MAVQTVPTKGNLMNTKKSLALAKNGYELLDRKRNILIREMMTLI
                     DHANAIQQKIDDAYAEAYTALQTANVTNGFCEDISNAIPYENGLKLDSRSVMGVEIPI
                     LTIEEREDHNYLHYGLRTTNSAIDKVYTKFTEVKNLTVELAEIENSVYRLADSIKKTQ
                     KRANALKNIMIPRFEETVKFISDALDEKDREEFSRLKVIKKKKQKDAALKKAKELSAE
                     SA"
     gene            complement(1010..2389)
                     /locus_tag="PLS64_05385"
     CDS             complement(1010..2389)
                     /locus_tag="PLS64_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HRL43295.1"
                     /translation="MIIDYVGVKEINGSLIVMDGVKGVSNEEIVDIRLDNGTMRQGRV
                     VQIEGERIVVQVFEGTRRISLKNTRTRLTGHPMEMPLSPEIIGRVLDGAGKPIDGLGD
                     IFPVKKANINGTPINPVSRVYPKNYINTGISTIDTLMTLIRGQKLPIFSGSGMKHNDL
                     AVQIVRQAKISGANSSNFGVVFAAMGVQKDVAEYFKKSFEESGVLSRVVMLLNLSNDP
                     IIERTLTPRCALTIAEYLAFELDMHILVIMTDMTSYAEALREFSSSKGEIPGRKGYPG
                     YLYSDLASLYERAGMIKGHNGSVTQIPILTMPNDDITHPIPDLTGYITEGQIVLDRSL
                     QGQGLYPPVNVLPSLSRLMKDGIGEGYTRADHQPLANQLFASYAKVIDARSLASVIGE
                     EELSPTDKKYIEFGRLFESKFVNQGFNENRSIEQSLDLGWELLSTLPRAELDRVDDAL
                     LDQYYKGDK"
     gene            complement(2408..4162)
                     /locus_tag="PLS64_05390"
     CDS             complement(2408..4162)
                     /locus_tag="PLS64_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HRL43296.1"
                     /translation="MENNNVIYGINGPVVTVKNTDSFEMMEMVHVGKQKLVGEIIGIT
                     DDITTIQVYEETTGLKPGDPVEGTGAPMNVLLGPGIIDNIFDGIERPLKAIEEEAGAF
                     INRGSSVSALDDKKLWNVTMKVKVGDKLEGGQIYATLPETPIIEHRLMVPPELSGEVV
                     KVNPNGEYKLFDTVVVIKDDEGVEHNLTLCQQWPIRTSRPVKERLTSSVPLITGQRVI
                     DTLFPIAKGGTAAIPGGFGTGKTMTQHQLAKWCDADIIIYVGCGERGNEMSQVLEEFS
                     ELIDPKSQRPMTDRTVLIANTSNMPVAAREASIYTGITLGEYYRDMGYHVAMMADSTS
                     RWAEALREISGRLEEMPAEEGFPAYLPSRLAEFYERGGRAEVLSGGEGSVTLIGAVSP
                     QGSDFSEPVTQNTKRFTRCFWALDKALAYSRHYPAINWMDSYSEYFNDLDPWFRENLG
                     EDFIEYRNRISALLQEESSLMEIVKLIGSDVLPDDQKLVIETARVIRVGFLQQNAFHA
                     DDTYVPLEKQKLMMKTILHLHAKAKDIVAQNIPLSKILNLGLFDKLTKMKYDIPNSKP
                     EMFDDYIKEIDEKLAAIS"
     gene            complement(4205..4801)
                     /locus_tag="PLS64_05395"
     CDS             complement(4205..4801)
                     /locus_tag="PLS64_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HRL43297.1"
                     /translation="MENSKNKTDNFLKAIKKYADEQRSAMQTEVAQLKEEKLKEATEK
                     GRHDSEKYIKDKLEESRNRQTGILAKKIQEGQKKLFLERAEMTESVFKKAEEKLVEYT
                     KTSDYSNSLVKSAKEVAKLFADNDCVVYVNERDMKNSDKIKAVFAGSTEVVADKSIKI
                     GGIKGFCKAMNIVADETLDSKLYAQKEWFIENSGLSVL"
     gene            complement(4841..5149)
                     /locus_tag="PLS64_05400"
     CDS             complement(4841..5149)
                     /locus_tag="PLS64_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HRL43298.1"
                     /translation="MKFFLISDNVDTKMGMRFAGIEGVVVHQEDEVRSELEKAVNRDD
                     IAVILITETLVSLCPDLIYDLKLNRKQPLIVEIPDRHGNGRTKDSITKYVQDAIGVKL
                     "
     gene            complement(5186..5611)
                     /locus_tag="PLS64_05405"
     CDS             complement(5186..5611)
                     /locus_tag="PLS64_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HRL43299.1"
                     /translation="MLFNLIMLAVPVVFLCASAILAVKAFEHGKNRKKTVLTQICSFA
                     AVFAFCMVCPIVASAATGDAAAATSGMADGLAYIGAALATGLSCIGGGIAVGNAAPAA
                     IGATSEDPKAFGKAIIFVVLGEGIAIYGMLVSILLIFAR"
     gene            complement(5633..7567)
                     /locus_tag="PLS64_05410"
     CDS             complement(5633..7567)
                     /locus_tag="PLS64_05410"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HRL43300.1"
                     /translation="MAVSTMQAVSIIGLTRDIDNVISVLGESGVFHPDDVSSFYSNTK
                     DFTHLQSKNIYAEPLNTLKATLSLTKRKFNLVDVSDFNPTFKEIELFTNQINSDIEVL
                     ADDRLFVEEQLMQAKENLVETTHFVGLGVEIEKVLTLKYISSRFGRLPKESFEKLSAY
                     KDNPYVDFIVCSEDKSHYWGVYFAPIDKTEEIDRIFSGLYFEKCDVLGVNDTPRIHLK
                     KLESLIPHLEEKLKEAQKRVDDYVDKYEVRICKYLSKLEELNLYAKIRTKALQYSKSF
                     IIVGWVPTEDAKPLRRRLKKIASVNVDFSDGKTEIAKSPPVKLKNCFLAKPFEFYTEM
                     YGVPKYNEIDPSLFIAITYVIIFGIMFADLGQGICVSIVGALMWKLRKMKIGKILVPC
                     GISSAIFGCVFGSVFGFEHVLDPLYKALFGLDEKPIEVMSSGSIVMILLAAVAIGISL
                     VVVAMGLNIYSSFKQGDVGRALFGSSGCAGLVFYCSIIFGVAGQLVLGLQVFSLAYIL
                     CLIVLPYLLIFFGEPLGLLIAGEKDWQPESWGGYILEHAIESIEFLLEYVTNTVSFLR
                     VGAFVLVHAGMMTVVFVLAQTAPAVAYWPVVVLGNVFVMVLEALLVAIQVLRLEYYEM
                     FSRFYSGEGRPYEPVKLNID"
     gene            complement(7600..>8242)
                     /locus_tag="PLS64_05415"
     CDS             complement(7600..>8242)
                     /locus_tag="PLS64_05415"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRL43301.1"
                     /translation="IDVNKLANATNYEEFLNATQSSQFYDILKQFSPDEKNRLPISDI
                     ENKLYNLVFGKLTKLIKATTKGQEKHELINFFFTINDYNIFSRILRLKKYYKLSPDDI
                     KANLLLNYTNLSEKTIDMMCKAESSGEIFSIMQNTHNGRLIDKIGYVYASDISPRVKY
                     RLARKNMHFSNNPSVVMISYIFVAETELMNIVTLIEGTRYELDNKITQSLLIK"
BASE COUNT         2403 a         1800 c         1589 g         2450 t
ORIGIN      
        1 caacggtatt atcgcatggt gttgggcgta tcaatcaatt aacagcataa gcgtaccaat
       61 aaaacaggga acggacatac ttggaattgt tatggcaaag gcatcatttg gtatgtttgc
      121 ctaccttatt tcagggattg caatagttgt ttttgcagaa tgtattgttt cggtatttat
      181 aaaataccga acaaaaaaga aacaacagct tattcacaac atctaaaaca aaacggattg
      241 gctcaataaa aatgccaatc cgttttatta tgttgacaca ttaaaacagc ccgaccaaat
      301 tacggtcggg ctgataattt atgcgctttc agcacttaat tctttagctt ttttcaaagc
      361 agcatctttt tgctttttct tcttgattac cttgagtcgg ctgaattcct cacgatcctt
      421 ttcgtcaagt gcgtcggaaa tgaacttaac tgtttcctca aaacggggaa tcataatgtt
      481 cttgagagcg tttgctctct tctgagtttt cttgattgaa tcggcaaggc ggtaaacgct
      541 gttctcaatc tcggcaagct caactgtaag atttttaact tcggtaaatt tcgtataaac
      601 cttatcaatt gccgaatttg ttgtgcgcag accgtagtgt aggtagttat ggtcctctcg
      661 ttcctcaata gtgagaatcg gaatttcaac acccataaca cttcttgaat caagcttcaa
      721 gccgttctca tagggaatag cgttagaaat atcttcacaa aagccgttgg taacatttgc
      781 cgtttgaagt gctgtgtaag cctctgcata agcgtcatca attttctgct ggattgcgtt
      841 tgcatggtcg ataagcgtca tcatttctct gatgagaatg ttacgctttc tgtccaacag
      901 ttcatagccg tttttggcga gtgcaaggga tttcttagta ttcataagat tacccttggt
      961 aggtactgtc tgtactgcca taaatgctca cactcctttc ttcaaacgat tatttgtcac
     1021 ccttatagta ctgatcaagt aaagcatcgt caacacggtc aagttctgct ctcggcaatg
     1081 ttgaaagcaa ttcccaacca aggtcaagac tctgttcaat acttctgttt tcattaaagc
     1141 cctgattaac gaactttgat tcaaacaatc tgccaaactc aatgtacttc ttatcagtag
     1201 gagaaagctc ctcttcaccg ataacagatg caagcgatct tgcgtcaatt acctttgcat
     1261 aagaagcgaa aagctggttt gcaagaggct ggtgatcggc tcttgtataa ccctcaccga
     1321 taccgtcctt cataagacgg gaaagggaag gaagaacatt aacaggcgga tacaatccct
     1381 gtccctgaag tgaacggtca agaacaatct gtccctctgt aatgtaacct gtaaggtcgg
     1441 ggataggatg agtaatatcg tcgttaggca ttgtcaaaat cggaatctgt gtaacagaac
     1501 cgttgtgacc tttaatcata cctgcacgct cataaagtga tgcaaggtca gaataaagat
     1561 agccggggta accctttctg cccggaattt caccttttga tgacgagaac tcacgaagag
     1621 cctcggcata agatgtcata tcggtcataa taacgagaat gtgcatatca agctcaaatg
     1681 caagatattc agcaattgta agagcacatc tcggtgtaag agttctttca atgataggat
     1741 cgtttgaaag gttcaaaagc attacaactc ttgaaagtac gccgctttct tcaaagctct
     1801 tcttaaagta ttctgcaaca tctttttgaa cacccattgc ggcaaataca acaccgaagt
     1861 tagagctgtt tgcaccgctg attttagcct gtcttacaat ctgaacggca aggtcattat
     1921 gtttcatacc tgagccggag aagataggca gcttctgacc tctgataaga gtcataagcg
     1981 tatcaatggt tgaaatacca gtgttgatgt agtttttagg ataaacacgg gaaacagggt
     2041 tgataggagt accgttgata ttagcctttt taacagggaa aatatctccg agtccgtcaa
     2101 taggtttgcc cgcaccgtca agtacacggc cgataatttc aggggaaaga ggcatctcca
     2161 taggatgacc tgtaagtctt gttcttgtgt ttttaaggct gattcttctt gtaccctcaa
     2221 aaacctgaac aactattctt tcgccctcaa tctgaacgac acgaccctga cgcattgtac
     2281 cgttatcaag acgaatgtca acaatttcct cgttggaaac gcccttaaca ccgtccataa
     2341 ctataagaga gccgttaatt tctttaacgc ctacataatc tataatcatt attgcgttcc
     2401 ctccttatta agaaattgca gcaagctttt cgtcaatttc cttaatgtaa tcgtcaaaca
     2461 tttcaggctt gctgttagga atatcatact tcatctttgt aagcttatca aacagaccaa
     2521 ggttaagaat cttggaaagc ggaatattct gagcaactat atccttagct ttggcatgaa
     2581 gatgaagaat ggttttcatc ataagctttt gcttttcaag cggaacataa gtatcatcgg
     2641 catggaaagc gttctgttgt aagaaaccga cacgaattac tcttgctgtt tcaattacaa
     2701 gcttctggtc atcggggagt acatccgaac caatgagctt tacgatttcc ataagtgaac
     2761 tttcttcctg taaaagtgcg cttattctgt ttctgtattc aataaaatcc tcaccgagat
     2821 tttccctgaa ccacggatca aggtcgttaa aatactcgct gtagctgtcc atccagttaa
     2881 tggcgggata atgtcttgaa taagcaagag ccttatcaag agcccagaaa catcttgtaa
     2941 aacgctttgt gttctgtgtt acaggctcag agaagtctga accctgcgga gaaaccgcac
     3001 cgataagcgt tacagaacct tcgccgccgc tgagaacctc ggcacgaccg ccacgctcat
     3061 agaactcggc aagtcttgaa ggaaggtacg cagggaaacc ttcttcggca ggcatttctt
     3121 caagtcggcc tgaaatctca cggagagcct cagcccaacg ggatgttgag tcagccatca
     3181 tggcaacatg gtaacccata tctctgtaat attcgccaag agtaatacct gtgtaaattg
     3241 aagcctcacg agccgcaaca ggcatatttg atgtgttggc gataagtacg gttctgtcgg
     3301 tcataggacg ctgtgacttt gggtcaataa gctctgagaa ttcctcaaga acctggctca
     3361 tctcgttacc acgctcaccg caaccgacat agatgatgat atcggcgtcg caccactttg
     3421 caagctggtg ctgagtcatt gttttacctg taccgaaacc gcccggaata gctgctgtac
     3481 cgccctttgc aatagggaag agagtatcaa taactctctg acccgtgata agcggaacag
     3541 atgatgtaag tctttcctta acaggacggg aagttctgat tggccactgc tgacaaaggg
     3601 taaggttgtg ctcaacgcct tcgtcatcct tgattacaac aactgtatca aagagcttgt
     3661 actcaccgtt gggattaacc tttactactt cacccgaaag ctcaggcgga accataagtc
     3721 tgtgttcaat gataggagtt tcggggagag tagcgtaaat ttgaccgccc tcaagcttgt
     3781 caccgacttt aaccttcatt gtaacattcc acagcttctt gtcatcaaga gcagaaaccg
     3841 aagaaccacg gttaataaac gcacctgctt cttcttcaat agcctttagc ggtctttcga
     3901 taccgtcaaa gatgttgtca ataataccgg ggccgagaag aacattcata ggagcaccgg
     3961 taccctcaac gggatcgccc ggtttaagtc ctgttgtttc ctcataaacc tgaatagttg
     4021 tgatgtcgtc tgtaataccg atgatttcac cgacaagctt ttgcttgcct acatgaacca
     4081 tttccatcat ctcaaagctg tcggtatttt ttacggtaac aacaggtccg tttattccat
     4141 atataacatt gttgttttcc atattattac catctctttc aaaagaaatt aacagtcagc
     4201 aagattaaag cacactcagt ccggaatttt caataaacca ttctttctgt gcatagagct
     4261 tgctgtcaag agtttcatcg gcaacaatat tcattgcctt gcaaaagcct ttgattccgc
     4321 cgattttaat tgatttgtcg gcaacaacct cggtgcttcc tgcaaaaact gccttgattt
     4381 tgtctgagtt cttcatatct ctttcgttga cataaacaac acagtcgttg tctgcaaaaa
     4441 gcttagcaac ttcctttgca ctttttacaa ggctgtttga gtaatctgat gtttttgtgt
     4501 attcaacaag cttttcttca gcctttttaa atacgctttc agtcatttca gctctctcga
     4561 gaaacagttt tttctgacct tcctgaattt tctttgcaag aatacccgtc tgtctgtttc
     4621 ttgattcttc aagtttgtcc ttgatatatt tttcgctgtc atgtctgcct ttttcggttg
     4681 cctctttcag cttttcttct ttaagctgag caacctctgt ctgcatggca cttctttgtt
     4741 catcagcata ttttttaatg gctttcagaa agttatctgt tttgtttttg gagttttcca
     4801 ttaaaaataa cctccctgaa taaattcaga tatgtgataa ttacagcttt acgccgattg
     4861 cgtcctgaac atattttgta atactgtcct tggttcttcc gttaccgtga cggtcgggaa
     4921 tttcaacaat aagcggctgt ttgcggttaa gcttgaggtc ataaataagg tcgggacaga
     4981 gcgaaacaag tgtttccgta ataagaataa ctgcaatatc atccctgttc acagcctttt
     5041 caagctcact gcgtacctca tcctcctgat gaacaactac gccttcaata cctgcaaatc
     5101 tcatacccat tttggtatcg acattatcac ttataagaaa aaatttcata ataatcaatc
     5161 cttaacgaaa cttaaatcgc ttaaattatc ttgcaaagat aagaagaatt gaaacgagca
     5221 taccgtaaat agcgatacct tcaccaagta caacgaagat gattgcttta ccgaatgcct
     5281 taggatcttc acttgtagca ccgatagcgg caggagcagc gttaccaacg gcaataccgc
     5341 cgccgataca tgaaagacct gttgcaagag cggcaccgat atatgcaaga ccgtcagcca
     5401 tacccgatgt agctgcagcg gcgtcacctg tagcagcgct tgctacgata gggcatacca
     5461 tgcagaatgc aaatacagca gcgaatgagc aaatctgagt aagaactgtt ttctttctgt
     5521 tctttccatg ctcaaaagcc tttactgcaa gaattgcaga tgcacaaagg aatactaccg
     5581 gaacagctaa cataattaaa ttgaataaca taataatacc tccaaaaaat tatcaatcaa
     5641 tatttaattt aacaggttcg taaggtctgc cttcaccgga atagaaacgg ctgaacatct
     5701 cgtaatattc aagtcttaac acctgaattg caacaagaag cgcctcaaga accattacaa
     5761 aaacattacc taaaacaact acgggccagt atgctactgc gggagctgtt tgggcaagta
     5821 cgaaaacaac cgtcatcata cctgcgtgta caagaacgaa tgcaccgact cttaaaaagc
     5881 ttaccgtgtt tgtaacatac tcaagcaaaa attcgattga ctcgatagca tgctcaagaa
     5941 tatatccgcc ccagctttcg ggctgccagt ctttttcacc ggcgataaga agtccgagag
     6001 gctcaccaaa gaagatgagc agatagggga gaacgataag gcaaagaatg tatgcaaggg
     6061 aaaatacctg cagacccaat acgagctgtc ctgcaacacc gaaaataatt gaacagtaga
     6121 atacaagacc tgcacaaccg cttgatccga acaaggctct tcccacatct ccctgcttga
     6181 aggaactgta aatattaagt cccatagcaa ccacaacaag cgaaatacct attgcaacag
     6241 ccgcaagaag aatcattaca attgagcctg atgacattac ttcaatcggt ttttcatcaa
     6301 gtccgaagag tgctttgtaa agaggatcga ggacatgctc aaatccgaac acacttccga
     6361 acacacagcc gaatatcgca gaagaaattc cgcacggcac aagaatctta ccgattttca
     6421 ttttcctgag tttccacata agcgcaccga caattgatac acaaatgccc tgaccgaggt
     6481 cagcaaacat tatgccgaat ataatgacat aagtgatagc gataaagagt gacggatcaa
     6541 tttcgttata tttcggaaca ccgtacatct ctgtgtaaaa ttcaaacggt tttgcaagga
     6601 aacagttttt gagtttgaca ggcggcgatt ttgcaatctc cgtcttgccg tctgaaaaat
     6661 ctacattgac gctggcaatt tttttgagtc tgcgtctgag aggcttagca tcctctgtag
     6721 gaacccaacc tacaatgata aagcttttgc tgtattgcaa tgcttttgtg cggattttgg
     6781 cataaagatt aagctcctca agcttagaaa gatatttaca gattctaacc tcatacttgt
     6841 caacataatc gtcaactcgc ttctgagctt ctttaagctt ctcctcaaga tggggaataa
     6901 ggctctcaag ttttttcaga tgaattctcg gtgtgtcatt aactccgaga acatcacatt
     6961 tttcaaagta caatcctgaa aagattctgt caatttcctc ggttttgtca atcggagcaa
     7021 aataaacgcc ccaataatgt gatttatcct cggaacagac aataaaatcc acataaggat
     7081 tatctttata ggctgaaagc ttttcaaagc tttccttagg cagtctgcca aagcgtgaac
     7141 tgatatactt taaggttaaa accttttcaa tttcaacccc aagtccgaca aagtgtgttg
     7201 tttcaacaag attttccttt gcctgcatca actgctcttc aacaaacagc ctgtcatctg
     7261 caagaacttc aatgtcgctg tttatctgat ttgtaaacag ctcaatttct ttaaaggtcg
     7321 gattaaagtc acttacatca acaagattaa acttgcgttt tgtaaggctt aatgttgctt
     7381 taagagtgtt aagaggttca gcatagatgt tttttgattg cagatgtgta aaatcctttg
     7441 tgttgctgta gaaagatgaa acatcatcgg gatggaacac acctgattca ccaagtaccg
     7501 atattacatt atcaatatcc cttgtaagtc cgataatgct tacggcctgc atggttgaaa
     7561 cagccaaatt ataatcacct cttcttgaca gtcaaataat catttaatga gcaatgactg
     7621 tgtaatttta ttgtcaagtt catatcttgt gccctcaatc agagtgacaa tattcatcag
     7681 ctctgtttcg gcaacaaaaa tatatgaaat cattacaacc gaagggttgt tggaaaagtg
     7741 catatttttc cttgcaagcc tgtattttac tcttggcgaa atatcgcttg catagacata
     7801 tccgatttta tcaatcaatc ttccgttatg cgtattctgc ataattgaga atatctcgcc
     7861 cgaagattcc gccttacaca tcatgtcaat ggtcttttcg ctgagatttg tgtaattaag
     7921 caagaggttt gctttgatat catcgggaga aagcttataa tactttttca atctcagaat
     7981 tctcgagaaa atattataat cgttaatcgt aaagaaaaag ttgataagct cgtgcttttc
     8041 ctgtcctttt gttgtagctt taatcagttt tgtcagtttt ccgaacacca agttatacag
     8101 cttgttctca atgtccgata ttggaagtcg atttttctca tctggagaaa attgcttgag
     8161 aatatcgtag aattgggagg actgtgtggc gttcagaaac tcttcataat ttgttgcatt
     8221 ggcaagcttg ttgacatcaa tt
//
LOCUS       DAOMVE010000074         8181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2275_length_8181_cov_3.462589, whole genome shotgun sequence.
ACCESSION   DAOMVE010000074 DAOMVE010000000
VERSION     DAOMVE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_8181_cov_3.462589"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..367)
                     /locus_tag="PLS64_05420"
     CDS             complement(146..367)
                     /locus_tag="PLS64_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43302.1"
                     /translation="MNFSYSQVLEFKRILDEKFGVYLHFHDACGGQYFSFDEAEKDEV
                     YQFLTSYFANIGGKINIADNKLTFTVDKA"
     gene            complement(380..1573)
                     /locus_tag="PLS64_05425"
     CDS             complement(380..1573)
                     /locus_tag="PLS64_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HRL43303.1"
                     /translation="MAKRNFGDEFGFQTRAVHTGNDVDSETGAIKRPITMANSYELPY
                     DPSDLNWSSAGKNLYTRNGGSNQQYLQEKLASLEGGEDCVVLASGVAALSGLFFALLK
                     SGDHVVFSSVTYIAVYRLLNELLKDKFGVETTIVDTTDIEKVKAAIRPNTKLIHIETP
                     GNPTLSISDIEKIAELAHANGALLSVDNTFASPFNQRPIELGADFTVESLTKYINGHG
                     DAMGGAIIGKKEYLDIIRAQSQINLGATISPFNAWLIMRGSVTLPLRMKQHNENALKV
                     ARYLKTVKGVSFVAYPGLEDHPNHDVAVKQMKNGFGGVLSFGLKADHDTYNRFVTHLK
                     VITSAVSLGHDESLIVFLGENDERQYLYPEEFHNGFFRFSVGIEDAEDIIEDLRQAFE
                     KENLI"
     gene            complement(1940..2353)
                     /locus_tag="PLS64_05430"
     CDS             complement(1940..2353)
                     /locus_tag="PLS64_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43304.1"
                     /translation="MNCRLVFFAARKTSFCERALQKSFSELDLELVKTCFATDSEQLG
                     NELTDAFKKLNAVFVVGGLGFDDEKGIKSIISRALCETPVNDCKKLKNENGDDGYVVR
                     AANQILILLPDEPEQIESIMQGPLRKYIMANSVMQ"
     gene            complement(2365..4350)
                     /gene="srtB"
                     /locus_tag="PLS64_05435"
     CDS             complement(2365..4350)
                     /gene="srtB"
                     /locus_tag="PLS64_05435"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HRL43305.1"
                     /translation="MMNNQTIERGIDMDVEIFSLTGKNSADLSQTSGEIAKKLEQNGF
                     SVTKVKSVSPSYSKIISALNELAKSEKAPDQVVIAEALTTKDSTSFRKKFAEVVAASE
                     KYENTPVPKDYWRKRNLDFLDAKKRKADKEEMEQLEDKYRMFRKKSRIFSLKDMGNGY
                     RGYCFMYRGIQVAVLPKSALAGENPEDMVCLACIRAKSNFENSAIDYPNGFSDREFVP
                     AKTGFVNNFIPMRGDGSKEVTRKCVVIVSFLVFLTALSLLFYNMIYLSLRNAELNGEI
                     QRIAHSVDDGETTPEKKKDDTINWDKLLKINDEIVGWIQMKDTHIDYPVLWHKADSTP
                     QQYYLNHNYKNEWDGFGSVFVDYRSTKGTDGKNLVLHSHHIQDGSMFGDLMKFGGTTG
                     DLDFYKEVPTFRFDTPKGKGTYKIISVFKTNTLTAHGDFFNYMISDFENDKDFMNYVY
                     NVRVRSLFNCPVDVNEDDELVTLSTCSYEFTNFRTVVVARKVRAGESTKVDVSKASLN
                     KNAVWPQVYYSSYGGTRPTVTDFDTAYKKGQITWYDGDYSFKNQKVTKKTEATTATDT
                     KGQVVTQKPQPTTEAKVYCNVTFLNYDGSALSTQKVEYGKSAVVPKTVPKKPSDEYYT
                     YTFEGWDTTYDYTKVTANLSIAPKFKATLKPEYANAQ"
     gene            complement(4340..5521)
                     /gene="thiI"
                     /locus_tag="PLS64_05440"
     CDS             complement(4340..5521)
                     /gene="thiI"
                     /locus_tag="PLS64_05440"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HRL43306.1"
                     /translation="MKEILLLKDGEIVLKGLNRRSFEDVLKKNLKHALKSAGHFEITS
                     AQSTIYVKPLDDDADLEKACEIVSRVFGIIAFSRAAVCEKTIESVLETAPKYLENQLK
                     NIKTFKVEAKRSDKKFPLKSPEICREVGGEILSKFPHLKVDVHNPDLVVNVEIRDFGA
                     YVHGEPVRGAGGIPVGTGGRAAILISGGLDSPVAAYMMAKRGIRLTAIHFASPPYTSP
                     RAEDKVVRLLRRVSRYAGDMVMYTVPFTKLQEKIKDECPEELFTIIMRRLMMQISEKI
                     AEKYECQALITGESLGQVASQTIGALSCTDEATDMLVFRPLIGMDKQEIIDIAYKIDT
                     YDISIEPYEDCCTVFTPKHPRTRPVLKYVKEAQEKPNFGPLVEEALQNLKVTRISADD
                     E"
     gene            complement(5561..6688)
                     /locus_tag="PLS64_05445"
     CDS             complement(5561..6688)
                     /locus_tag="PLS64_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRL43307.1"
                     /translation="MEHYLDNSATTKVSQKAAEKAFEIMTENYGNPSSLHLRGMYAEQ
                     ELVKARKEVARKLGATADEIYFTSGGTEANNLAIFGVAEAKKRRGKRIVVSAVEHSSI
                     MESAKKLEDNGFDVVYIMPREDGTIHKEDVLEKVDENTILVSVMCVNNETGAIMNVTD
                     IFSAVKAKNSDVICHTDAVQAFGKISLSASRLHADLISVSGHKIHAPKGVGAIFVKKG
                     VRLVPLHYGGEQEKKLRPGTEAIPLIAAMGVACGEFNIDKNYDYVSKLNSYAKDKLLK
                     IDGVSLNSPENALPYVLNISAGKVRSETMLHFLENLEVYVSSGSACAKGKPSYVLESM
                     NIGRDRADSALRISFSKFNTEADIDALCYGIEKGLKTLAHK"
     gene            6838..7293
                     /gene="spoVAC"
                     /locus_tag="PLS64_05450"
     CDS             6838..7293
                     /gene="spoVAC"
                     /locus_tag="PLS64_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HRL43308.1"
                     /translation="MISKKEYSTIVKNNSPKSKCFVNGTKAFFIGGFICLIGQVLTDC
                     YSMLDLSKDDAKTLTSVTLIFIGILLTAIGVYDKIAKHGGAGTLVPITGFANAVSSPA
                     IEFKSEGYVAGLGAKLFIIAGPVIVYGVTASVVFGAVLWVLHMFGITLF"
     gene            complement(7361..8089)
                     /locus_tag="PLS64_05455"
     CDS             complement(7361..8089)
                     /locus_tag="PLS64_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRL43309.1"
                     /translation="MDTKEILLKLRTKSGFSQDELAEKLFVTRQAVSRWENGDTVPNT
                     ETLKLLSKLYNVSINTLLGSPRDLICQCCGMPLEDEIISREKDGFMNEDYCKWCYADG
                     NYMYSDMDDLIDVCVKNMANEEHSEKEVREYLLRTLPKLDYWKRYKELGGNKAFEEFK
                     NVLIDEINALNIEGKYVNLGYRLPNGNTVKFLDDNATYLGNQLECEFGGDRYFGIVAN
                     TDFILISTYEENLKNPELVLYKKR"
BASE COUNT         2123 a         1831 c         1666 g         2561 t
ORIGIN      
        1 tgaggggaat ataaattgac ataataagca ctttgcaaaa ttataagtct aatcgtactt
       61 gccgtgctta ttatatcacg gcaatcgtga cagaataatg acaaacaaga gaatgttttg
      121 aaaatctttt aaaaaaattt tctgattacg ctttatctac tgtaaatgtc aatttattgt
      181 cggcaatatt gattttaccg ccgatatttg caaaataaga ggttaaaaat tgatatactt
      241 cgtccttctc tgcttcatca aacgaaaaat actgtcctcc gcaggcatcg tgaaaatgta
      301 gatacactcc gaatttttcg tcaagaatcc tcttgaattc aagcacctgt gaatatgaaa
      361 aattcataat aagctctcct taaatcaggt tttctttttc aaaagcctgt cttaaatctt
      421 cgatgatatc ttcggcatcc tcaataccaa cgctgaaacg gaaaaatccg ttgtggaatt
      481 cttcgggata aagatactgt ctttcatcgt tttcaccgag aaatacgata aggctttcat
      541 cgtgaccgag tgatacagcc gatgtgatta ctttaagatg tgtaacaaaa cggttgtagg
      601 tatcgtggtc ggctttaagt ccaaacgaaa gcactccgcc gaacccgttt ttcatctgct
      661 taactgcaac atcgtgattc gggtgatcct caagtcccgg atatgcaaca aagcttacac
      721 ctttgaccgt tttcagataa cgagcaacct tgagggcatt ttcgttgtgc tgtttcatac
      781 gcaacggaag tgttaccgag cctctcataa tcagccatgc attgaacggg ctgattgttg
      841 cgccgaggtt aatctgcgac tgcgcacgga taatgtcgag gtattctttt ttgccgatga
      901 ttgcaccacc cattgcatcg ccgtgaccgt tgatgtactt tgtaaggctt tcaacggtaa
      961 agtctgcacc gagttcaatc ggtctttggt tgaacggaga agcaaatgtg ttgtcaaccg
     1021 acaaaagtgc gccgtttgca tgggcaagct ctgcaatctt ttcaatgtcg gaaattgaaa
     1081 gagtagggtt gcccggtgtt tcaatgtgaa tgagctttgt gttcggacgg attgccgcct
     1141 taactttttc aatgtcggtt gtgtcaacaa ttgttgtttc aacaccgaat ttgtctttga
     1201 gaagttcgtt cagaagtctg taaaccgcaa tatatgttac gcttgaaaat acaacatggt
     1261 caccgctttt gagcaatgca aagaaaagtc ccgacagagc cgcaacaccg cttgcaagaa
     1321 caacgcaatc ctctccgccc tcaagggacg caagcttttc ctgaagatac tgctgatttg
     1381 aaccgccgtt gcgtgtgtag aggtttttgc ctgcactgct ccagttgagg tcgctcggat
     1441 catacgggag ttcatagctg tttgccattg tgataggtct tttgattgct cctgtttcgc
     1501 tgtcaacatc attgcccgtg tgaacagccc ttgtctgaaa tccgaattca tcaccaaaat
     1561 ttctttttgc cataactgta cttcctttct tagcatgatt tttatgtgct tgattatgta
     1621 tgtgttttat ttctactatt cctatgtatt tagtatgttt taattatagc accatttttt
     1681 gcattgtcaa gattaatttt ataaatggcg aacacggttt gcgaccgccg cctgtggcgg
     1741 ataaagggag catagcgctg tctgaaatat agtcgcaagg ctacccctac tgattccata
     1801 taaaacaaaa attgcatcag aaccttattc gggcatctga tgcaatcttt ttatctaact
     1861 atcttctcaa attgacagcg gtggcacacc gccgactgta attttacatt ttcaattttc
     1921 aactttcaat tcgcaatgct cattgcatta ccgagtttgc cataatatat tttctgagcg
     1981 gtccctgcat aatggattca atctgctcgg gttcatcggg cagaagaata agaatctgat
     2041 ttgcggctct gacaacatat ccgtcatcgc cgttttcatt tttcagcttt ttgcagtcat
     2101 taacaggggt ttcacacaaa gcacgggaaa ttatgctttt tattcccttt tcatcgtcaa
     2161 atccgagtcc gccgacaacg aaaaccgcat tcagtttttt gaaagcgtct gtaagttcat
     2221 ttccgagttg ttcggaatcg gttgcaaagc aggttttgac gagttcaagg tcaagctcgg
     2281 aaaaggattt ttgcaaagct ctttcgcaaa acgaggtttt gcgagcagcg aaaaatacaa
     2341 gcctgcaatt cattttccgt cctcctactg tgcgtttgcg tattcgggtt tcagcgttgc
     2401 cttgaatttt ggtgcaatgc tcaaattagc cgttaccttt gtatagtcat aggtggtgtc
     2461 ccaaccctca aaggtgtagg tataatactc atcactcggt tttttcggta cggtcttagg
     2521 cacaaccgcc gacttgccgt actcaacctt ttgcgttgac aaagctgaac cgtcgtagtt
     2581 aaggaaggtt acattgcaat aaacctttgc ctctgttgta ggctgtggct tttgtgttac
     2641 aacctgacct tttgtatctg ttgcggttgt agcctcggtt ttcttggtta ccttttggtt
     2701 cttaaagctg taatctccgt cataccaggt aatctgacct ttcttgtaag ctgtgtcaaa
     2761 gtctgtgacg gtaggtcttg taccgccgta ggatgagtaa taaacctgcg gccatactgc
     2821 attcttatta agcgatgcct ttgaaacatc aacctttgtt gactcgcccg ctcttacctt
     2881 tcttgcaaca acaaccgttc tgaagtttgt gaactcatac gaacaggttg aaagtgttac
     2941 aagctcatcg tcctcgttta catcaacagg acaattaaag agtgaacgaa cacggacatt
     3001 gtaaacatag ttcataaaat ccttgtcatt ttcaaagtcg cttatcatgt agttgaagaa
     3061 atcgccgtgt gcggtaagag tgtttgtctt gaacacggag ataatcttat aagtaccttt
     3121 gccctttgga gtgtcaaaac ggaaggtcgg aacttctttg tagaaatcga gatcccccgt
     3181 tgttccgccg aacttcataa gatcaccgaa cattgaaccg tcctgaatgt ggtgagagtg
     3241 aagaacaagg ttcttgccgt ctgtaccctt tgttgaacgg tagtcaacaa atacgctgcc
     3301 gaagccgtcc cactcattct tgtagttgtg gttaaggtaa tactgttgcg gtgtgctgtc
     3361 tgccttatgc caaagaacgg ggtagtcaat gtgggtatcc ttcatctgaa tccagccgac
     3421 aatttcatcg ttaattttca gaagtttatc ccaattgatt gtgtcatcct ttttcttttc
     3481 gggtgttgtt tcaccgtcat caacgctgtg agcaattcgc tgaatttctc cgttaagctc
     3541 ggcatttctc aggctgaggt aaatcatatt gtagaacaac agcgaaagtg ccgtaagaaa
     3601 cacgagaaac gaaacaatta caacgcattt tcttgtaact tcctttgaac cgtcaccacg
     3661 cataggaatg aagttgttga caaagcctgt ctttgccgga acaaattccc tgtcgctgaa
     3721 accgttagga tagtcaattg cagaattttc aaagtttgac tttgctctta tgcaggcaag
     3781 acaaaccata tcttcgggat tttcacctgc aagagccgac tttggaagaa cggcaacctg
     3841 aataccacgg tacataaagc agtagccacg atagccgttg cccatatctt taaggctgaa
     3901 aattcttgac tttttacgga acattctgta cttgtcctca agctgttcca tctcttcctt
     3961 gtctgcctta cgctttttgg catcgagaaa atcaagattt ctctttcgcc aatagtcctt
     4021 cggcacggga gtattctcat acttctcgga tgccgcaaca acctcggcaa actttttgcg
     4081 aaacgatgtg ctgtcctttg ttgtaagagc ctctgcaatc acaacctgat caggcgcttt
     4141 ttcgcttttc gcaagctcgt taagtgcaga aataatttta gaatatgaag gtgaaacgct
     4201 ttttactttg gttacggaaa agccgttttg ttcaagcttt tttgcaattt caccgcttgt
     4261 ttgagaaagg tcagcggaat ttttgcccgt taaggaaaaa atctcaacat ccatgtcaat
     4321 tcccctttca atcgtttgat tattcatcat cagccgaaat tcttgtaact tttaggttct
     4381 gaagagcctc ctcaacaaga ggtccgaagt tcggcttttc ctgagcctct tttacatatt
     4441 tgagaacagg tcttgtacgg ggatgctttg gtgtgaacac ggtacagcag tcctcatacg
     4501 gctcaattga tatatcgtag gtatcaatct tgtaagcaat gtcaataatc tcctgcttat
     4561 ccatacctat gagcggtctg aacacgagca tatcggtcgc ctcatctgta cacgaaagcg
     4621 caccgattgt ctggcttgca acctgaccga ggctttcgcc tgtgatgagt gcctgacatt
     4681 cgtacttttc ggcaatcttt tctgaaatct gcatcatcag gcggcgcata atgattgtga
     4741 aaagttcctc gggacattca tccttgattt tctcctgcag ctttgtaaac ggcacggtgt
     4801 acataaccat atcgcctgca tatctgctga ctctgcggag aagtctgaca accttgtctt
     4861 cggctctggg gcttgtgtag ggcggacttg caaagtgaat tgcagtaagt ctgatacctc
     4921 tttttgccat catataagcg gcaacgggag agtcaagacc tccgctgatg agaattgcgg
     4981 ctctgccgcc tgtgccgacc ggaataccgc ctgctcctct taccggctca ccgtgaacat
     5041 aagcgccgaa gtcacggatt tcaacattta caacaaggtc ggggttgtga acatcaacct
     5101 tgaggtgcgg aaactttgaa aggatttcac cgccgacctc tctgcaaatt tcgggagatt
     5161 taagcggaaa cttcttgtcg cttctctttg cctcaacctt gaaggttttt atatttttaa
     5221 gctgattttc aagatatttc ggagcggttt caagaacgga ttcgattgtt ttttcgcaaa
     5281 cggcggctct tgaaaaagcg ataattccga acacccttga aacgatttcg caagcctttt
     5341 caagatctgc gtcatcatca agcggtttta cataaattgt ggactgtgcg gatgtaatct
     5401 caaaatggcc tgcactcttt aatgcgtgtt tgaggttctt tttgaggaca tcttcaaatg
     5461 aacggcggtt aagtcccttg agaacaattt cgccgtcttt caaaagtaaa atttccttca
     5521 taaaatgtat tcctttgttt taatatgtat tcatacgatt ttatttgtga gcaagggttt
     5581 tcagtccctt ttcaataccg taacaaagtg cgtcgatatc tgcctctgtg ttaaattttg
     5641 aaaagctgat tctcagtgca ctgtcggctc tgtcacgacc gatattcatt gactcaagca
     5701 cataactcgg tttgcccttt gcacaggcac ttcccgagga cacatagacc tcaagatttt
     5761 caagaaaatg gagcatggtt tcgctcctga cctttcctgc cgagatattc agcacatacg
     5821 gaagtgcgtt ttcgggggag ttgaggctca caccgtcaat ttttaatagc ttatcctttg
     5881 cgtagctgtt aagctttgaa acataatcgt aatttttatc tatgttaaat tcaccgcagg
     5941 caacacccat tgcggcaatc agcggaattg cctctgtacc ggggcggagt tttttctcct
     6001 gctcaccgcc gtagtgaagc ggaacaagcc gtacaccctt tttcacaaag attgcaccca
     6061 cacccttcgg ggcatggatt ttatgaccgc tcacgctgat taaatccgca tgaagccttg
     6121 acgcagaaag cgagattttg ccgaacgcct gaacggcatc cgtgtggcag attacatcgc
     6181 tgttcttcgc ctttacggca gaaaaaatat ccgtaacatt cataattgcg cccgtttcat
     6241 tattgacgca cattacagac acaagaattg tgttttcgtc aaccttttca agcacatctt
     6301 ccttgtgaat tgtgccgtcc tcacggggca taatgtagac aacatcaaag ccgttatcct
     6361 caagtttttt tgcggattcc ataatactgc tgtgttcaac ggcagacaca acaatccgct
     6421 ttcctctgcg ttttttagcc tcggcaacac cgaaaattgc aaggttattc gcctctgtac
     6481 cgccgcttgt aaaatagatt tcgtcggcag ttgcaccaag ctttcttgca acctcttttc
     6541 gagcctttac aagctcctgc tctgcataca ttccacgcaa atggagtgac gaaggattgc
     6601 cgtaattttc ggtcattatc tcaaacgctt tttctgccgc cttttgcgac accttcgtag
     6661 ttgcgctgtt atcgagataa tgctccatta ctttaccctc atttcgtttt acatagctgt
     6721 acatattaat aagtatacta caacagtcaa aaaaaataaa gtgataattc aaacataatt
     6781 gaaatttata cggcaaaaat atatgtaaat taattaacgg tgcaaaggtg gcattttatg
     6841 atttcaaaaa aagaatatag cacgattgtg aaaaataatt caccgaaaag taaatgtttt
     6901 gtgaacggga ctaaggcatt tttcatcggc ggtttcatct gccttatcgg tcaggttctg
     6961 acggactgtt attcaatgct tgatctcagc aaggatgatg caaaaacgct gacatcggta
     7021 acgctgattt tcataggaat tttgctgaca gcaatcggtg tttacgataa aatcgccaag
     7081 catggcggtg caggtacgct tgtgccgatt acaggttttg caaatgctgt ttcatcgcct
     7141 gcaattgaat tcaaatccga gggatatgtt gcaggactcg gcgcaaagct gttcatcatt
     7201 gcaggacctg tcatagttta cggtgttacc gcatctgttg ttttcggtgc tgttttatgg
     7261 gtactgcata tgttcgggat taccttattc tgacttttca ataatttaaa ctgcaaaaag
     7321 aggtgtcgca aacgctgaca cctctttttc tgttatgttg ttatcttttc ttgtacaaaa
     7381 caagctccgg atttttaaga ttttcctcat aagttgaaat caggatgaaa tcggtatttg
     7441 caacaatgcc gaaatatctg tcaccgccga actcacattc aagctgattg ccgagatatg
     7501 ttgcgttgtc atccaaaaac ttaaccgtgt tgccattcgg aagtctgtat cccaagttga
     7561 catattttcc ctcaatattt agggcattta tctcatcaat aagaacattt ttgaattcct
     7621 caaaagcctt gtttccgccg agttccttat atcttttcca atagtcgagc tttgggagtg
     7681 ttctcaacag atactcacgc acctcttttt cggaatgttc ctcgtttgcc atatttttga
     7741 cgcagacatc aatcaaatcg tccatatcgc tgtacatata atttccgtcg gcataacacc
     7801 acttgcaata atcttcattc ataaagccgt ctttctctct gctgataatc tcatcttcca
     7861 acggcatacc gcaacattga caaatgagat ctctcggaga accgagcaag gtattgattg
     7921 acacattata gagctttgaa agcagtttca atgtttccgt attcggaact gtgtcgccgt
     7981 tttcccaacg agaaacagcc tgtcttgtta caaacagttt ttcagccagc tcatcctgag
     8041 aaaagcccga ttttgttctt aatttcagta aaatttcttt tgtatccata gccgtcacct
     8101 cttgcttata ttataatgca gagcgtgatt tttcacaagc aacttgctgt tgcacggcga
     8161 gccgccgtcc ccaattcgtg t
//
LOCUS       DAOMVE010000075         8178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2279_length_8178_cov_3.228733, whole genome shotgun sequence.
ACCESSION   DAOMVE010000075 DAOMVE010000000
VERSION     DAOMVE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8178
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2279_length_8178_cov_3.228733"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLS64_05460"
     CDS             complement(<1..526)
                     /locus_tag="PLS64_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL43310.1"
                     /translation="MIELLGFLGQYICFFILHVCGGGSFPKPLTEKQERECLEKSANG
                     DIAARNMLVEHNLRLVAHIIKKYYGTQSEQDDLVSIGTIGLIKAINTFDMNKNIKLSS
                     YASRCIENEILMHFRNAKKSAQDISLNETIDTDKDGNPLTLLDIMSVDDNIIDNLDLK
                     LNSKKLGQFIREELN"
     gene            745..2139
                     /locus_tag="PLS64_05465"
     CDS             745..2139
                     /locus_tag="PLS64_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HRL43311.1"
                     /translation="MEKIFKLKENGTNVKTEIIAGITTFLAMAYILAVNPSILSAAGM
                     DPNAIFAATAVSAAFATLIMAFYANYPVVLASGMGLNAYFAYTVVPQLAEQGITNPWQ
                     VALTAILVEGIIFIILSIFKFRETLVNKVPQNLKYGISAGIGLFITIVGLKGAGVITT
                     DAYYDGKAVQSSTLVKLGDVGSAPVVLALVGILIVATLWHYRVKGSILIGIIATWVLG
                     IIAQLCGWYQGASLIPDFANYSVFGPVQNMATETFFKFDFGYVAQHALNFAVIVFAFL
                     FVDLFDTVGTLIGVAQEGNLLNEKGELPRVGRALMSDALGTCLGACLGTSTVTSYVES
                     TTGVAEGGRTGLTALTSAILFILAIPLYPIFLAIPSFATAPALVFVGLLMIKNVTKMD
                     FDSDIADTVGGFVAIIFMPFAYSIATGIMFGILTWVILKIVTGKIKEIHPIMWVAFAL
                     FAVRIVTLICGVSS"
     gene            2297..2722
                     /locus_tag="PLS64_05470"
     CDS             2297..2722
                     /locus_tag="PLS64_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRL43312.1"
                     /translation="MKISTKGRYALRLMVDLAQCKQGENVALKEVAARQGISLKYLEQ
                     IITVLTKAGYVLSARGSNGGYRLAHEPEYYTVGMILRLTEGSLAPVACLEGNENTCDR
                     REQCSTLFVWEKLQKAIDDVVDNITLADIIANSADNYVI"
     gene            3181..4482
                     /locus_tag="PLS64_05475"
     CDS             3181..4482
                     /locus_tag="PLS64_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRL43313.1"
                     /translation="MSKLSERNLKFETKQLHIGQETADPVTDARAVPIYATTSYVFHN
                     SQHAADRFALKDAGNIYGRLTNPTEDVFEKRIAALEGGTAALAVASGAAAVSYALQAL
                     AKAGDNIVAAKTIYGGTYNWLEHTFSEYGVTTKFVDPDEENAFENAIDENTKAVFIES
                     LGNPNSNIIDIEEVANIAHSHKIPLVIDSTFATPYLLRPFEYGADIVVHSATKFIGGH
                     GTALGGVIVESGKFDWAASGKFPQFSEPNASYHGAVFTEAAPGAALVTYVRAILLRDT
                     GATLSPFHAFMFLQGLETLSLRVERHVENALKVVDFLSKNPKVENVNHPSLPDSKYNA
                     LYKKYFPKGAGSIFTFEIKGDAETAKKFIDHLEVFSLLANVADVKSLAIHPASTTHSQ
                     LSEAELAEQGIKPNTIRLSIGTENIDDIIYDLQEAFDSLDD"
     gene            4519..5451
                     /gene="cysK"
                     /locus_tag="PLS64_05480"
     CDS             4519..5451
                     /gene="cysK"
                     /locus_tag="PLS64_05480"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRL43314.1"
                     /translation="MSKVYTSVTELIGRTPLIELVNIEKKYDLKAKVVAKLELFNPAG
                     SVKDRVARAMVEDAEKSGKLKKGSTIIEPTSGNTGIGLASVATAKGYKTILTMPETMS
                     VERRNLLKAYGAEIVLTDGTKGMSGAIAKAKELEKEIDGGIILGQFENPANPKAHFDT
                     TGPEIWEDTDGNVDFFVAGVGTGGTLSGVGNFLKSKKADVKVVAVEPQTSPVLSEGHG
                     GPHKIQGIGAGFVPDTLDTKVYDEILPIANEAAFSNANDIAKTEGILVGISSGAALAA
                     AIELAKREENAGKTIVALFPDTGERYLSTGVFNS"
     gene            5567..6175
                     /locus_tag="PLS64_05485"
     CDS             5567..6175
                     /locus_tag="PLS64_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRL43315.1"
                     /translation="MKVKICGITSAEDIKIVNACKPDFAGFVMFFPKSKRNISPETAK
                     SLIETLDKNVLPVAVTVSPTLEQVKTAYDCGFDYIQIHGEVEEDVLSNPYLKVIRAFN
                     VSDLEKFDEYRMNPNIVGYVFDAHEPGSGKTFDWTMLENLPRDDKLFMLAGGLNPETV
                     AKAVKAVKPDGVDVSSGVENSNGNGKDFEKVKKFIISARSEN"
     gene            6297..6593
                     /gene="rpsF"
                     /locus_tag="PLS64_05490"
     CDS             6297..6593
                     /gene="rpsF"
                     /locus_tag="PLS64_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRL43316.1"
                     /translation="MAVTANYETVMIISMKKGEEGIQAIVEKFKALIEKHATLKNVDE
                     WGKRKLAYLINKESEGYYVLFDFESEAEFPAELDRIYKITEGVMRSMIIKKEVE"
     gene            6633..7133
                     /gene="ssb"
                     /locus_tag="PLS64_05495"
     CDS             6633..7133
                     /gene="ssb"
                     /locus_tag="PLS64_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005938982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRL43317.1"
                     /translation="MLNRVILMGRITADPELKTTNTGVSVTSFSIAVDRNYVKQGEER
                     KADFFNIVCWRSTAEFVCRYFGKGSLIAVEGQLQSRQFQAKDGSNRYVVEVVADNVSF
                     TGERRDNNAGGYNQSYGGNQSYGAPSYGGNQAYHQEPAQQAPASYQSGSNSDFQDMPL
                     DDDLPF"
     gene            7179..7418
                     /gene="rpsR"
                     /locus_tag="PLS64_05500"
     CDS             7179..7418
                     /gene="rpsR"
                     /locus_tag="PLS64_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRL43318.1"
                     /translation="MADRPMNRGRKARKKVCGFCVDKVENIDYKDIARLRRYMSERGK
                     ILPRRVTGTCARHQRELTVAIKRARHLALLPYTAD"
     gene            complement(7506..7781)
                     /locus_tag="PLS64_05505"
     CDS             complement(7506..7781)
                     /locus_tag="PLS64_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HRL43319.1"
                     /translation="MKYIIKPTNQFKKDFKKIKKQNKDLNILKKVIDMLANDEILPDK
                     YRDHLLIGNYKGKHECHLEPDWLLIYEYLDDELILFLTRTGSHSDLF"
     gene            complement(7778..8047)
                     /locus_tag="PLS64_05510"
     CDS             complement(7778..8047)
                     /locus_tag="PLS64_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRL43320.1"
                     /translation="MAMTNINIRIDADKKLKAQELFSSLGLDMTTAINLFIQQSLEFN
                     GIPFTIRKYNAETEAAFAEVEEMKKHPELYKSYSSADELFEDLDK"
BASE COUNT         2401 a         1646 c         1697 g         2434 t
ORIGIN      
        1 cgttaagttc ctctcttatg aactgaccga gcttttttga attaagcttc aaatcaagat
       61 tgtcaattat gttatcgtca accgacataa tatcaagaag ggtgagaggg ttgccgtcct
      121 tgtcggtatc aattgtttcg tttaaagaaa tatcctgagc acttttcttt gcatttctga
      181 aatgcataag aatttcgttt tcaatgcatc tgctcgcata agaacttaat tttatgtttt
      241 tgttcatgtc aaatgtgttg attgccttta tcagaccgat tgtgccgatt gatacaaggt
      301 catcctgttc gctttgtgtt ccgtaatatt tctttattat gtgtgcgaca agcctcagat
      361 tgtgttcaac aagcatattt cttgccgcaa tatcgccgtt tgcacttttt tcaagacatt
      421 cacgctcttg cttttctgtt aaaggtttcg gaaacgaacc gcctccgcaa acatgaagaa
      481 tgaaaaaaca tatatattgt cctaaaaatc cgagtaactc aatcataaca gcacccctta
      541 aactaaaatt tatcgttata taattttatt cgtgaaatgt tcaataattg cctgtacctg
      601 attttcctca aaaaacttca caaagcaatc aaaaacttga tttttgcaat gaatttgtat
      661 aatataccac ttgaaatttt ggcaagaata aactacaatt taactatgtc gtgtttcgac
      721 aaaatatttt aggggggctt tcttttggaa aagattttca aactgaaaga aaacggcact
      781 aatgtcaaaa ccgagattat tgccggcatt acaacattcc ttgcaatggc ttacatactc
      841 gctgtaaacc cgagtattct ttctgccgca ggaatggatc cgaatgcaat ctttgcagca
      901 acagctgtat cggctgcttt cgcaacactc atcatggcat tttatgccaa ctatcctgta
      961 gttcttgctt ccggtatggg actgaatgca tattttgcat acacagttgt tccgcagctt
     1021 gcagagcagg gtatcacaaa tccatggcag gtagctttga cagctatcct tgttgagggt
     1081 attattttca ttatcctctc aatcttcaaa ttccgtgaaa cacttgtaaa caaggttcct
     1141 cagaacctca agtacggtat ttcggcaggt atcggtcttt ttatcacaat tgtaggtctt
     1201 aagggcgcag gcgttatcac aacagacgct tactatgacg gcaaggctgt tcagtcatca
     1261 actctcgtta agctcggtga tgttggttct gctccggtgg ttcttgcact tgtcggtatc
     1321 ctcattgttg ctacactctg gcactacaga gttaaaggct caatccttat cggtatcatt
     1381 gcaacatggg ttctcggcat cattgctcag ctttgcggtt ggtatcaggg tgcatcgctc
     1441 attcctgatt ttgcaaacta ctctgtattc ggacctgtcc agaacatggc aacagaaact
     1501 ttcttcaagt ttgatttcgg ttatgtggca cagcatgcgc ttaactttgc cgttatcgta
     1561 tttgcattcc tctttgttga cctcttcgat acagtaggta cacttatcgg tgttgctcag
     1621 gagggtaatc tcctcaatga aaagggcgaa cttccccgtg taggtcgtgc tcttatgtct
     1681 gacgcactcg gtacatgcct tggtgcttgc ctcggtacat caacagtaac atcttatgtt
     1741 gagtcaacaa caggtgttgc agagggtggc agaacaggtc ttacagctct tacttctgca
     1801 attctcttta tccttgctat tccgctttat ccgattttcc ttgctattcc ttcatttgca
     1861 acagctcctg cactcgtatt tgtaggtctt cttatgatta agaatgttac aaagatggat
     1921 tttgactcag acattgccga tacagtcggc ggtttcgtag caatcatctt tatgccgttt
     1981 gcatattcaa tagcaacagg tatcatgttc ggtatcctta cttgggttat tctcaagatt
     2041 gttacaggca aaatcaaaga aattcatccg attatgtggg ttgcgtttgc tttgtttgca
     2101 gtaagaattg taacactgat ttgcggtgta tcgtcgtaac ttcatatata aactctgcgg
     2161 gggcgttttt cgctcccgct ttttgtgttt tcttacaagt taaataggtt attgttgtgc
     2221 aacaggctca ttgacatata cagccgttta tgatatgctt taatcatatt attaatatat
     2281 gaatagggtg gttaatttga aaatttcaac aaagggcagg tatgcgctcc gacttatggt
     2341 tgaccttgct cagtgtaaac aaggggaaaa tgtagcccta aaggaggttg ccgcccgtca
     2401 gggaatatcg cttaaatacc ttgaacagat tattaccgta cttacaaagg caggttatgt
     2461 gctcagcgca agaggttcaa acggaggata tcgtcttgct catgaacccg aatattatac
     2521 tgtcggtatg attttaagac ttaccgaggg cagtcttgca cccgtggctt gccttgaggg
     2581 taatgaaaat acctgtgatc gtcgtgaaca atgttcaacg ctttttgtgt gggagaaact
     2641 ccagaaggct attgacgatg ttgttgacaa cattactctt gccgacatta ttgcaaattc
     2701 ggcagataac tatgtaattt aatataatcc gaaagcatcc tttacgggtg cttttgttgt
     2761 ttacacagca atttagtagg taatgcggtg ttttgccgtt tgcaaatgat gcttttgaat
     2821 attagtttta aaaaatcaga aaaaatttca aattgactgc ttaacggttg aaatttaaaa
     2881 aacggcttga atatcggctt tttcgacctt ttcaataatc aaaacccagc aaacctacta
     2941 aaccaatagg aaagataaga aaaaatttca aaaaacctat tgacaaataa aaaatctgtg
     3001 gtatgatata gacaatccaa ttaaaaccca ccaatcaaat aggaaatatg ggaaaggtga
     3061 tccaaatgaa aaatctcaga ttcaaacgat gttgcagtaa tgcacgaatg tgatacggga
     3121 cacatcaaat ccgcaacata acttctgaaa ttaaaattaa aatgaaatga ggacatcaaa
     3181 atgagtaaat taagcgaaag aaatcttaaa tttgaaacaa aacagcttca catcggtcag
     3241 gaaacagcag accctgtaac agacgcaaga gcagttccga tttacgcaac tacttcttat
     3301 gtattccaca acagtcagca cgctgcagac cgctttgcac tcaaagatgc aggtaacatc
     3361 tacggcagac ttacaaaccc gacagaggat gtttttgaaa agagaattgc agctcttgag
     3421 ggcggcacag cagctcttgc agttgcatca ggtgcagcgg cagtttcata tgcacttcag
     3481 gctcttgcaa aagcaggcga caacatcgta gccgcaaaga caatctacgg cggcacatac
     3541 aactggcttg agcatacatt ctcagagtac ggtgtaacaa caaagttcgt tgatcctgac
     3601 gaagaaaacg catttgaaaa tgcaattgac gaaaacacaa aggctgtttt cattgaatca
     3661 ctcggtaacc ccaattcaaa tattattgat attgaagaag tagcaaacat tgctcattca
     3721 cacaagattc cgcttgtaat cgacagcaca ttcgctacac cttatcttct cagaccgttt
     3781 gagtacggcg cagatatcgt tgttcattcg gcaacaaagt ttatcggcgg tcacggtacg
     3841 gctctcggcg gtgtaatcgt tgaaagcggc aagtttgact gggctgcatc aggcaagttc
     3901 ccacagttct cagagcctaa cgcatcatat cacggtgcag tatttacaga ggctgctccg
     3961 ggcgctgctc ttgtaactta tgttcgtgcc attctcctca gagatacagg cgctacactt
     4021 tcaccgttcc acgcatttat gttcctccag ggccttgaaa cactttcact tcgtgttgaa
     4081 cgccatgttg aaaatgcact caaggttgtt gacttcctct ctaagaaccc gaaggtagaa
     4141 aatgttaatc atccttcact tcctgacagc aagtacaacg cactttacaa gaaatacttc
     4201 ccgaagggtg caggctcaat cttcacattt gaaatcaagg gtgacgctga aactgcaaag
     4261 aaattcatcg accacctcga agtattctca cttcttgcaa atgttgcaga tgttaaatct
     4321 cttgcaattc accctgcatc aacaactcat tcacagctca gcgaggcaga gcttgcagaa
     4381 cagggcatta agccaaacac tatcagactt tcaatcggca cagagaatat cgacgatatt
     4441 atctatgacc ttcaggaagc atttgattca cttgatgact gattttaatt tgttgaattt
     4501 tgaaaggact tgatttttgt gagcaaagta tatacaagtg taacagaact tatcggcaga
     4561 acaccgctta tcgaactcgt taatatagaa aagaaatatg acctcaaggc aaaggttgtt
     4621 gcaaagcttg aactctttaa ccctgccggc agtgttaagg acagagtggc aagagctatg
     4681 gttgaggacg ctgaaaagag cggtaagctc aaaaagggtt caacaatcat tgaaccgaca
     4741 tcaggtaaca caggcatcgg tcttgcatct gtagctactg caaagggcta caagacaatc
     4801 cttacaatgc ccgaaacaat gagcgttgag agaagaaacc tcctcaaggc atacggtgct
     4861 gaaatcgttc ttactgacgg tacaaagggt atgagcggtg caattgcaaa ggctaaagag
     4921 cttgaaaagg aaattgacgg cggcattatc ctcggtcagt ttgagaatcc tgctaaccct
     4981 aaggctcatt ttgatacaac gggtccggaa atctgggagg atacggacgg caatgttgat
     5041 ttcttcgttg caggtgtcgg cacaggcggt acactcagcg gcgtgggtaa cttcctcaag
     5101 tcaaagaagg ctgatgtaaa ggttgttgca gttgagccgc agacttctcc cgtactttcg
     5161 gaaggtcacg gcggtcctca taagattcag ggtatcggtg ctggctttgt tccggatact
     5221 ctcgatacaa aggtatatga cgaaattctt ccgattgcta acgaagccgc tttctcaaat
     5281 gcaaatgata ttgcaaagac agaaggtatc ctcgtaggta tctcatcagg cgccgctctt
     5341 gcagccgcaa ttgagcttgc caagcgtgaa gaaaatgcag gtaagacaat cgttgcattg
     5401 ttccccgaca caggtgagcg ttacctttca acaggcgtat ttaacagcta ataagtaatt
     5461 ggattacttc ccgatattga caaaggacag ccacgcagac tgtcctttgt ctttttttat
     5521 tgcaaccgtt aattcgatat ggtacaatta ttttgtaggt gagtttatga aagttaaaat
     5581 ttgcggaatt acatccgccg aagacattaa gatagtaaat gcctgcaaac ccgattttgc
     5641 gggctttgta atgttctttc caaagagtaa acggaatatt tctcccgaaa ccgccaagag
     5701 cctgattgaa acgcttgata aaaatgtttt gccagttgcg gtaactgttt ctccgactct
     5761 tgaacaggtg aaaactgcat atgactgcgg ttttgattac attcagattc acggtgaggt
     5821 tgaagaagat gtgctttcaa atccatatct caaggtaatt cgtgccttta atgtgagcga
     5881 ccttgaaaag tttgacgaat acagaatgaa cccgaacatt gtaggctatg tttttgacgc
     5941 acacgaaccc ggcagcggaa aaacctttga ctggacaatg cttgaaaatc tgcctcgtga
     6001 tgataaattg tttatgcttg caggaggtct gaatcccgaa accgtggcaa aagctgtaaa
     6061 agccgttaag cctgacggag ttgatgtaag ttcgggtgtt gaaaattcaa acggcaacgg
     6121 taaagatttt gaaaaagtga agaaatttat tatatctgct cgaagtgaaa attaataaaa
     6181 cagtaaaaaa atgttgacaa accgtgattt tatgttataa taaatctgtt atgttgtgta
     6241 cataacaatc cttgcggtga aaaccgccat aatgtccaga aggaggtgca atattaatgg
     6301 cagtaacagc taattacgaa acagtaatga tcatttcaat gaagaagggc gaagaaggta
     6361 tccaggccat cgttgaaaag ttcaaggctc tcattgagaa gcatgccact ttgaagaatg
     6421 ttgacgaatg gggcaagaga aagcttgctt accttatcaa caaagagagc gaaggctatt
     6481 atgttctttt cgattttgaa agtgaggctg agttccctgc agagctcgac cgtatctaca
     6541 agatcacaga aggcgtaatg cgttcaatga taatcaagaa ggaagtcgaa taatcaattt
     6601 gtaggggtta acccgaacac gaaaggaaaa gtatgttaaa cagagtaatc ttaatgggca
     6661 gaattacagc tgaccctgaa ttaaaaacaa caaacacagg cgtaagcgtt acaagcttca
     6721 gcattgcagt tgatcgcaac tatgtaaagc agggcgagga gcgtaaagcc gacttcttta
     6781 acattgtatg ttggagaagt acagcggaat ttgtatgccg ttactttgga aagggttctc
     6841 tcattgctgt tgaaggacag ctccagagcc gtcagttcca ggctaaggac ggttcaaacc
     6901 gttacgttgt tgaggttgta gcagacaatg tttcgttcac aggcgaacgc cgtgacaaca
     6961 atgcaggcgg ctacaatcag tcctatggcg gcaatcagtc atacggtgct ccgtcttacg
     7021 gcggaaacca ggcttatcat caggaacctg ctcagcaggc acctgcatct tatcagagcg
     7081 gttcaaactc tgattttcag gatatgccgt tggacgatga tttaccattt taacttcaac
     7141 ttaatttaaa taattctcat tgaaaggaga acttcatcat ggcagacaga ccaatgaacc
     7201 gtggcagaaa agctcgcaag aaggtttgcg gcttctgtgt tgacaaggtt gagaatatcg
     7261 attacaagga tattgctcgt cttcgtcgtt atatgtcaga aagaggaaag attcttcctc
     7321 gccgtgtaac aggtacttgc gcaagacatc agcgtgagct tacagttgct atcaagcgtg
     7381 ctcgtcacct tgctttgctt ccttacacag ctgactaatt taataacagc ttttaacacc
     7441 gtacattttg tacggtgttt ttttatgcaa taaaagcagg gtatttttag taccctgctt
     7501 ttttgttaaa ataaatcgct gtgtgaacct gttcttgtaa gaaacagtat caattcatca
     7561 tctaaatatt cgtaaattaa cagccagtca ggctctaaat gacattcgtg tttgccttta
     7621 tagtttccta tcagcaaatg gtcacgatat ttgtccggaa gaatttcgtc atttgcgagc
     7681 atatcaatta ctttcttcag aatgttcaaa tctttgtttt gctttttaat ttttttaaaa
     7741 tcttttttga attgatttgt gggtttgatt atatatttca tttatccaaa tcctcaaata
     7801 attcatcggc actgctatag ctcttataaa gttcgggatg ttttttcatc tcttcaactt
     7861 cggcaaaagc ggcttcggtt tctgcattat atttgcgaat agtaaacgga ataccgttaa
     7921 attcaagtga ttgctgaata aaaagattga tggcggtagt catatcaagt cctaaagatg
     7981 aaaacagttc ctgtgctttc aattttttat cagcatcaat tctgatgttt atatttgtca
     8041 tagccataaa atcatctcct tgttattatg ttatcacaat gtaataacaa tgtcaacatt
     8101 atcttttgta tattttttgt tagttttttc tgtaatttac ctattgacaa ataccccaat
     8161 ggggtataca atcataac
//
LOCUS       DAOMVE010000076         8177 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2281_length_8177_cov_4.168924, whole genome shotgun sequence.
ACCESSION   DAOMVE010000076 DAOMVE010000000
VERSION     DAOMVE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_8177_cov_4.168924"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(382..813)
                     /locus_tag="PLS64_05515"
     CDS             complement(382..813)
                     /locus_tag="PLS64_05515"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRL43321.1"
                     /translation="MHARTKVTVRYAETDRMGIVHHSNYPIWYEIARNDYIKMFGISY
                     TDMENAGVMTPLLNLSCHFGIPALYEDELIIRTWVQTITAARIIFTYTVKRIEKDGSE
                     TELGYGTTEHGFVDSKTFRPTSIKKRFPKLYEDISAAVKHF"
     gene            complement(855..2786)
                     /gene="gyrB"
                     /locus_tag="PLS64_05520"
     CDS             complement(855..2786)
                     /gene="gyrB"
                     /locus_tag="PLS64_05520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRL43322.1"
                     /translation="MELSKVEQEYGADEIQVLEGLEAVRKRPGMYIGSTGPRGLHHLV
                     YEIVDNSIDEALAGYCTKIDVVIEKGDIIRVTDNGRGIPVGVNSKTGLPAVTVVFTVL
                     HAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVKVCDGEDVYFQRYERGKIVTDLQK
                     IGKSDVKGTEVRFKADPEIFKETTVYDYSVLERRLREQAFLNAGVHIELRDERDLENI
                     IQNNFVYEGGIKSFVEYIHKKRSLEVIHPDVIYFASRNADDTIAVEIALQYNESYNEN
                     LLTFANNIHTTDGGTHEEGFKRALTYVMNDYARKFGKLKDNDKNLSGEDVREGLTAIV
                     SVKLKEAQFEGQTKAKLGNTEVRAMVDRLMRDKLSVYLEENPAVAKVIFEKALASSRA
                     REAARKARENARRKSPLDSAQLPGKLADCQSKDSSETEIFIVEGDSAGGSAKGGRDRR
                     IQAILPLWGKMLNVEKARIDRVYGNDKLMPVITALGTGIGDEFDIAKLRYDKVIIMAD
                     ADVDGSHIRTLLLTFFFRYMRPLIEEGHVYIAQPPLFRISKGKEHKYAYSDLERDQIL
                     AQIEGKTEVQRYKGLGEMDSEQLWETTMNPETRLMLRVDLSDAEQADETFTILMGDKV
                     EPRRDFIEKNAKYVQNLDV"
     gene            complement(2936..3331)
                     /locus_tag="PLS64_05525"
     CDS             complement(2936..3331)
                     /locus_tag="PLS64_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43323.1"
                     /translation="MFRLPVCPHCHAIYRYGDVRKSLNTKIHTCYHCKKQFKTSFSGL
                     WILFLIAVVIGIFVNIFEMTAFSGTNVIALMITNIVLILFFLLFVPFFTKFRKFGADA
                     QKSENIQISKKQQKKQTNKNAVRSSNKRR"
     gene            complement(3343..4074)
                     /locus_tag="PLS64_05530"
     CDS             complement(3343..4074)
                     /locus_tag="PLS64_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43324.1"
                     /translation="MKMRKCPYCGRRISYTSSFASRRKAEYICQKCGKESKVAVDKKV
                     ILFFIVAVVISLAIMAGWILAGLVSNPLGILLVAIPLIIFTAVSPNFVHYEPYKKYRK
                     SMEAKKAGIAYSDNLLTSELDEDENYTFPSITPQQNDTDDNFEINTDVFNKIRAERNA
                     ARQRLDSNEISSDSSSFEKQEYVSVINNTSENHASSGATLKKIHSEMPQHHTVNRSRH
                     YINSEEASENKKTDDGNRYSVNRKF"
     gene            complement(4101..4373)
                     /locus_tag="PLS64_05535"
     CDS             complement(4101..4373)
                     /locus_tag="PLS64_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014115905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HRL43325.1"
                     /translation="MYLHLGQDTVITTETVIGIFDMDECTVSKKTRDYLTAAEKRGRV
                     VNVSFELPKSFVVCEKNGKITVYISQLSSKTLIKRSQSPYKELMYR"
     gene            complement(4373..5470)
                     /gene="recF"
                     /locus_tag="PLS64_05540"
     CDS             complement(4373..5470)
                     /gene="recF"
                     /locus_tag="PLS64_05540"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRL43326.1"
                     /translation="MKVIALKFENYRNLKDNIITPCDGVNIIYGDNAQGKTNLLEALW
                     FFCGGHSFRGSKDSEIINWDENFARIEMRFLGQEREQTAKILFRGGKKSVEINGVQKN
                     SAAALIERFCAVVFSPEHLSLIKRGPGERRKFIDSAICREKLKNAVVLSKYNRILNQR
                     NSLLKDIYRRPSLADTLPVWDEPLLKSGAVLVKNRIDYVKMLSDRAVEYHNGISKGKE
                     ELKIRYMSGYEASENDTVGEIYEKLKCKLEKHKSDDIRTGFTNYGPHRDDIEIIINGK
                     NARAFASQGQQRSAVLSLKLAEASVLRERMGEEPVILLDDVLSELDAKRQDFLLNELH
                     GCQVFITCCEKSNKEQLKDGKIFLLNNGEVS"
     gene            complement(5491..5703)
                     /locus_tag="PLS64_05545"
     CDS             complement(5491..5703)
                     /locus_tag="PLS64_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HRL43327.1"
                     /translation="MKTEKIKIDSEYIKLQDLLKLGGAVETGGQAKVVIQNGDVTVNG
                     EICTMRGKKMRVGDTAVFDGLEIIVE"
     gene            complement(5693..6799)
                     /gene="dnaN"
                     /locus_tag="PLS64_05550"
     CDS             complement(5693..6799)
                     /gene="dnaN"
                     /locus_tag="PLS64_05550"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRL43328.1"
                     /translation="MKISCLRSDLATAISNVSRAVSTKASIPALEGVLIKAYGNTLNI
                     SGYNLEIGITTDIEATIKEEGEIVVSAKLFLDIVRRLPEEIVFIETDERMVTYIACGK
                     VNYQIVGMSSVEYPDLPSFEQTDRITLNAKILRDMIRQTVYAVSENTAKPIYTGSLYE
                     IEDGVFKIIAIDGYRMAIRSENVDSESKNRFVVPGKTQHEVLKLLTDDEENTEIIIGQ
                     RHITFKIKNYRIISRLIEGTFIEYKSAIPKETKTEVVINTRTIIDAVERMSLLNNDRI
                     QSPVRCIFSDDEIKLSCTSAVGRANDVISVPIIGESVEIGFNNRYLLDALKNADTDEI
                     KLILNGSLTAMIVKPVKGDSYLSIVVPMRLANEN"
     gene            complement(7029..>8177)
                     /gene="dnaA"
                     /locus_tag="PLS64_05555"
     CDS             complement(7029..>8177)
                     /gene="dnaA"
                     /locus_tag="PLS64_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRL43329.1"
                     /translation="IFGTKFNIEFRTPANAPKKSKPSAAASITTSAATAPLLQSPDSS
                     SYEYTFDTFIVGSSNKFAHAACLAVATNPSHAYNPLFLYGNSGLGKTHLLYAIGNEIK
                     KNDPSKVICYIKGDDFTVELVESLRLAKMNEFRQKYRQADILLVDDVQFIGGKESTQE
                     EFFHTFNALYDARKQIVLTSDRPPKEIKTLEDRLRSRFEQDLIADIQPPDLETRIAII
                     KRKAELDGVEISDEVCEYVASKIKANIRQLEGTVKKIKAKYYLDGEKPTINSVQGIIS
                     DILNNDAPPEVTVERIIDEVARTYGVSADEIRSQKNRSANISNARHIAIYVTRELTTL
                     SMVAIGEEFGNRHYSTIIYTIQKVQKMMEKDRKVKEIIDDTIKNIRDR"
BASE COUNT         2118 a         1779 c         1582 g         2698 t
ORIGIN      
        1 agctcttgtt actgtctgtt caataattta taataatgtt attttgcgtt tttttaatta
       61 ttttagagtg taattttgtt tttacgtaaa gtaaattgta tttttatata gcaatgttat
      121 ttttaatctg tagtggagaa ccttgttctc cctttcgtgt gtgttgcaca cgatttcacg
      181 caatgcgtga aaatttaata cacggtttaa atcacttcaa aacatatgtg taaacaaatt
      241 cattcatcaa gcgggcggat gatatccgca catacgcata aattattcaa acaatcacaa
      301 ttgtatgctc gggtatagtt ttgcatactg caaactttct tctcgagttg ggtgcagcga
      361 cacgctgccg gcgactcgct gttagaagtg ttttactgcg gctgaaatat cttcgtagag
      421 tttcggaaag cgttttttga tgcttgtggg acggaatgtt ttggaatcta caaaaccgtg
      481 ttcggttgta ccgtagccaa gttcggtttc cgaaccgtcc ttttcaatac gctttaccgt
      541 gtatgtaaaa ataattcttg cggctgtgat agtctgcacc caagtgcgga taatcagctc
      601 gtcctcataa agagcaggaa ttccaaaatg acaactcagg ttcaaaaggg gagtcattac
      661 acctgcattt tccatgtctg tgtagctgat tccgaacatt ttgatatagt catttcttgc
      721 tatttcgtac caaatcggat aatttgagtg gtgtacaatt cccattctat ccgtttctgc
      781 gtaacgaact gtaacttttg ttcttgcgtg cataaaatca ccccgttttt aaaattttgg
      841 tttatcgtaa tatatcagac atcgagattc tgtacatact ttgcattttt ctcaatgaaa
      901 tctcttctcg gctcaacctt atcacccata agaattgtga aggtttcgtc tgcctgttct
      961 gcatcgctga gatcaacacg gagcataagt cttgtttcgg gattcattgt tgtttcccaa
     1021 agctgttcgg agtccatctc accaagacct ttgtatcgct gaacttcggt tttaccctca
     1081 atttgagcca gaatttggtc acgctcaagg tccgaatacg catatttatg ctctttgccc
     1141 tttgaaattc taaagagagg cggctgggca atgtaaacat ggccctcttc gataagcgga
     1201 cgcatatatc tgaaaaagaa agttaaaaga agggttctga tgtgcgaacc gtcaacatcg
     1261 gcatccgcca taatgataac cttgtcgtat ctgagctttg caatatcaaa ttcatcacct
     1321 atgcctgtgc cgagcgcagt gataacgggc ataagcttgt catttccgta aactctgtca
     1381 attcttgctt tttcaacatt gagcatttta ccccaaagag ggaggatagc ctgaattctt
     1441 ctgtcacggc cgccctttgc tgaaccgccc gcagagtcac cctcgactat gaagatttct
     1501 gtttcgctgc tgtcttttga ctggcagtcg gcaagcttac ccggaagctg tgcggagtca
     1561 agcggagatt ttcttcttgc attttccctt gcttttcttg cagcctctct tgctcttgac
     1621 gacgcaagcg ccttttcaaa aattaccttt gcaacagcag gattttcttc gagataaaca
     1681 gaaagcttat ccctcataag gcggtcaacc attgctctta cctcggtatt accgagtttt
     1741 gccttggtct gtccttcaaa ctgagcctct ttgagcttta cactgacaat tgcggtaagt
     1801 ccttcacgga catcttcacc gctgaggttc ttatcattgt ccttcaattt tccgaacttt
     1861 cttgcatagt cattcataac ataggtgagt gctcttttga agccttcttc atgagtaccg
     1921 ccgtcggttg tatgaatgtt gtttgcaaaa gtgagcaaat tttcgttgta gctttcgttg
     1981 tactggagtg caatttcaac cgcaatcgtg tcatctgcgt ttcttgacgc aaagtaaatt
     2041 acatcgggat gaataacttc aagagaacgc tttttgtgaa tatactcaac aaagctcttg
     2101 ataccgccct catagacaaa gttattctga ataatatttt caaggtcacg ctcgtcacga
     2161 agctcaatgt gaacacctgc attgagaaat gcctgttcac gaagtcttct ttccaaaacg
     2221 ctgtaatcgt aaaccgtggt ttctttaaaa atttcggggt cggctttaaa gcgtacttcc
     2281 gtacccttaa catcagactt gccgattttt tgaaggtcgg ttacaatttt tcctctttca
     2341 taacgctgga agtaaacatc ctcgccgtca caaaccttga cttcgagcca ctcggaaagt
     2401 gcgtttacaa cagacgcacc tacgccgtgc aaaccgcccg aaaccttgta accgcctccg
     2461 ccgaattttc cgccggcgtg gagaactgtg aatacaaccg ttacggcagg gagtcctgtt
     2521 ttgctgttca cgccgacagg aataccacgg ccgttatcgg ttactctgat tatatcgccc
     2581 ttttcgatga caacatcaat ttttgtacaa taacctgcaa gtgcttcgtc aattgagtta
     2641 tctacgattt cgtaaacaag gtggtgaagt cctctcggtc ctgttgaacc gatgtacatg
     2701 ccgggacgct ttcttacagc ctcaagtcct tcaagaacct gaatttcatc tgcaccgtat
     2761 tcctgttcaa cctttgaaag ctccataccc tcggattcgc cgtcaaattc aaccaatttt
     2821 tcagcctcaa tctctttttc gtaggcttca tcaatggctt cctcgtttat attttcgttg
     2881 gtttcatcaa tattttcatt catattttcg ttgttttcaa gattatccaa aacacttacc
     2941 tccgtttatt cgatgaacga acagcgtttt tgttcgtttg ttttttctgc tgtttttttg
     3001 atatttgaat attttcgctt ttttgagcgt ctgctccgaa ttttctgaat tttgtaaaaa
     3061 acggcacaaa aagcagaaaa aacagtataa gtacgatatt tgtaatcatc aacgctatca
     3121 catttgtgcc tgaaaaagcc gtcatttcaa agatattgac gaaaattcct atcacaactg
     3181 caatcaaaaa taaaatccaa agtcccgaaa atgaggtttt gaactgcttt ttgcagtgat
     3241 agcaggtgtg aatttttgta tttaagctct tccttacatc accgtaacga taaattgcgt
     3301 gacaatgcgg gcatacgggc agtctgaaca caatatcacc cgttaaaact ttctgttcac
     3361 ggaataacgg ttgccgtcat ccgttttttt attttccgaa gcttcctcgg aatttatgta
     3421 gtgtctgctt ctgttcaccg tatggtgctg gggcatttcg gagtgaatct ttttgagtgt
     3481 tgctccgctt gacgcatgat tctcacttgt attattaatc acggaaacat attcctgctt
     3541 ttcaaaagac gatgaatcgg aagaaatctc atttgaatca agtctttgtc ttgcggcatt
     3601 tctttcggct ctgattttgt taaatacatc cgtgttaatt tcaaaattat catcggtatc
     3661 attctgctga ggagtgatac tcgggaaagt atagttttca tcctcgtcaa gttccgaggt
     3721 aagaagattg tctgaataag cgatgcctgc tttttttgcc tccatagact tacggtattt
     3781 cttgtacggc tcatagtgaa cgaagttcgg tgaaactgcc gtaaaaatta taagcggaat
     3841 cgcaacgagt aaaattccga gaggattgct cacaagacct gcaagaatcc aacctgccat
     3901 tattgcaaga gaaattacca cagcaacaat aaaaaacaaa attaccttct tatcaactgc
     3961 aaccttgctc tcttttccgc acttttggca gatatattct gcttttcttc tgcttgcaaa
     4021 cgatgaggta tatgaaattc ttcttccgca gtaggggcat tttctcattt tcatacacag
     4081 aactcctttt tatatccgta tcaacggtac ataagctcct tatagggaga ttgacttctt
     4141 tttatgagtg tttttgatga aagctggctg atataaacgg taattttgcc gttcttttca
     4201 cagacaacaa aggatttcgg caattcaaac gaaacattta caactctgcc tcttttttcg
     4261 gcggcagtaa gataatccct tgtctttttt gacacggtac actcgtccat atcaaaaatt
     4321 ccgataaccg tttcggttgt gataacggtg tcctgtccga gatgaagata catcagctca
     4381 cctctccgtt gttcagcaga aagatttttc cgtctttaag ctgttcttta ttcgactttt
     4441 cacagcaggt aataaacacc tgacatccgt gaagttcgtt gagtaaaaaa tcctgtcttt
     4501 ttgcatcaag ctcagaaagc acatcatcaa gcagaataac gggttcttcg cccattcttt
     4561 cacgcaatac agacgcctcg gcaagcttca gcgacagaac cgcacttcgc tgttgtccct
     4621 gagatgcaaa agctctggca tttttaccgt ttattattat ttcaatatca tcacgatgag
     4681 gaccgtaatt tgtaaaaccc gtccttatat cgtcactctt gtgtttttca agcttacact
     4741 tgagtttttc ataaatttca ccgaccgtat cattctcgga ggcttcatat cccgacatat
     4801 aacggatttt cagctcttct ttgccttttg aaattccgtt atgatactca acggcacggt
     4861 cggaaagcat ttttacatag tcgattctgt tttttacaag cactgcgccg cttttcaaaa
     4921 gcggttcatc ccatacggga agtgtgtccg caagtgacgg ccgcctgtaa atgtctttca
     4981 aaagcgaatt tcgttgattg agaatcctgt tgtattttga gagaacaacc gcatttttga
     5041 gcttttcacg acagattgcg ctgtcaataa attttctccg ctcacccgga cctcttttta
     5101 taaggctgag gtgttcggga gaaaatacaa cggcgcaaaa ccgttcaatc agtgcggcgg
     5161 ctgaattttt ctgcacaccg ttgatttcta cggacttttt tccgcccctg aaaagaattt
     5221 ttgctgtttg ctcacgctcc tgaccgagaa aacgcatctc aatcctcgca aaattctcat
     5281 cccaattgat aatttcgctg tcctttgaac ctctgaagct gtgacctccg caaaaaaacc
     5341 acagtgcctc aagcaaattt gtctttccct gagcattgtc accgtaaatt atatttactc
     5401 cgtcacaagg agtgattata ttgtccttta aatttctgta attttcaaat ttaagagcaa
     5461 ttaccttcac ttacttcatc aatcccaaag ttattcaacg attatttcaa gaccgtcaaa
     5521 cacagccgtg tcgccgacac gcattttttt gcctctcatt gtgcagatct caccgtttac
     5581 ggtaacatct ccgttctgaa ttacaacttt tgcctgtccg ccggtttcaa ccgcaccgcc
     5641 gagtttaagt aaatcctgca attttatgta ttcgctgtca attttaattt tctcagtttt
     5701 catttgccaa cctcatcgga acaactatgc tcagatagct gtcaccctta acgggtttta
     5761 caatcatcgc cgtaagtgaa ccgttgagaa taagcttaat ctcatcggta tcggcatttt
     5821 tcaaagcgtc aagaaggtat ctgttgttaa agccgatttc aaccgactct ccgatgatag
     5881 gaacagagat tacatcattg gctctgccta ctgcagatgt gcatgaaagt ttaatttcat
     5941 catccgaaaa aatgcatctt accgggcttt ggattctatc gttgttcaaa agcgacattc
     6001 tttcaactgc atcaataatt gttcttgtat tgataactac ctcagttttt gtctctttcg
     6061 gaattgcaga cttgtattct atgaatgtgc cctcgataag acgggagata attctgtaat
     6121 ttttgatttt gaatgtgata tgtcgctgac cgatgataat ttccgtattt tcttcgtcat
     6181 cggtgaggag tttaagcacc tcatgctgag tttttcccgg aacaacaaaa cggtttttgc
     6241 tttcgctgtc tacattttca ctgcgaattg ccattctgta accgtcaatg gcaataattt
     6301 tgaatacacc gtcctcaatt tcgtaaagtg aacctgtgta aataggcttt gccgtgtttt
     6361 cgcttactgc gtagactgtc tgtctgatca tatcacgcag aatttttgcg ttgagagtga
     6421 ttctgtcagt ctgttcaaat gacggaaggt cgggatattc aacagatgac ataccgacaa
     6481 tctgataatt aacctttccg caggcaatgt atgtaaccat acgctcatcg gtttcgataa
     6541 agacaatctc ttcgggaagt cggcgtacaa tatcaagaaa cagctttgcg cttacgacaa
     6601 tttcgccttc ttctttgatt gttgcctcaa tatcggttgt tataccgatt tcaaggttat
     6661 aacctgaaat attcagtgtg ttaccgtatg cttttataag aacgccttca agtgcaggaa
     6721 ttgatgcctt tgttgacaca gctcttgaca cattggatat ggctgttgcc aaatcggagc
     6781 gcaaacatga aattttcatc gggtgcattc tctcctgtct ttttattaat atttttattg
     6841 tagtagtagt aggggatgtc aaaatgtcaa aaagcggtga gattccgcat tgacactgaa
     6901 aaaaagcata aacaaaagat tttttcgatt tgtttgcttt tgttgacaaa aatatttttt
     6961 agtaaatttt tcaacatcgg gttgaagaat gtttatggaa tgttgaaaaa tatgttaaat
     7021 aattcagtct atctgtcacg gatatttttg atggtatcgt caatgatttc tttgaccttt
     7081 ctgtcctttt ccatcatctt ctgaaccttc tgaattgtat atataatggt agaataatga
     7141 cggttgccga attcttcacc gattgcaacc attgaaagcg ttgtaagctc tcgtgtaaca
     7201 tatattgcaa tatgtcttgc gttgctgata ttggcactgc ggtttttctg tgaacgaatt
     7261 tcgtctgccg aaacgccgta ggttcttgca acctcgtcaa taatgcgttc aacggtaacc
     7321 tcgggcggag cgtcattgtt gagaatgtcg gaaattatgc cctgaaccga attaattgtc
     7381 ggtttttcac cgtcaaggta gtatttggct ttgatttttt taactgttcc ctcaagctga
     7441 cggatatttg ccttgatttt agatgcaaca tactcgcaga cttcgtccga aatttcaact
     7501 ccgtcaagtt ctgccttcct ttttataatg gcaattcttg tttcaagatc cggaggctgg
     7561 atatctgcaa taagatcctg ctcaaatctt gaacgcaaac gatcctcaag agttttgatt
     7621 tccttaggag gacggtccga tgtgagaaca atctgttttc ttgcgtcata aagtgcgtta
     7681 aaggtatgga aaaattcttc ctgtgtactt tctttaccgc cgataaactg aacatcgtcc
     7741 acgagcagaa tatccgcctg tctgtatttt tgtctgaatt cattcatctt ggcaagacgg
     7801 agcgactcaa caagctcaac ggtaaaatcg tcgcctttta tgtaacagat aacctttgac
     7861 ggatcgtttt tcttgatttc attgccgatt gcatacaaaa ggtgagtctt gccgagtccc
     7921 gagtttccgt aaagaaagag ggggttgtag gcatgtgacg gatttgttgc aactgcaaga
     7981 caggctgcat gagcaaactt atttgacgag ccgacaataa atgtgtcaaa ggtgtattcg
     8041 taagaactgc tgtcggggct ttgcaaaaga ggagcggttg ccgccgatgt tgtgattgat
     8101 gccgccgcag acggctttga ttttttcggc gcatttgccg gtgtgcggaa ctcaatattg
     8161 aactttgttc cgaaaat
//
LOCUS       DAOMVE010000077         8138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2302_length_8138_cov_2.952245, whole genome shotgun sequence.
ACCESSION   DAOMVE010000077 DAOMVE010000000
VERSION     DAOMVE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_8138_cov_2.952245"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1249
                     /gene="hisZ"
                     /locus_tag="PLS64_05560"
     CDS             83..1249
                     /gene="hisZ"
                     /locus_tag="PLS64_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRL43330.1"
                     /translation="MKKNLKMTPEGTKDFLFAECSAMDYICTSIEKVFVKGGFKRVIT
                     PALEFYDVFSIPSSGISQESMFKTTDNKGRLLVARPDSTLPIARMVSTRLKNNTLPVR
                     LYYKQAVYRNNPSLAGRRNEFLQMGVELLGAKGKRADLEILTSAIKSISAVADDFRIE
                     LGHAEVFNALSKELDIDNDYKEKIRVSIESKNYSALNNLLDKLKPCKTVSAIRSLPSL
                     FGGEEVFDEAYDICKGTGAENALDYLHTIYKELSVLELGNKLIIDLGLVQRNDYYTGI
                     VFSGYINGIGDAVISGGRYDDLLSEFDAPMGAAGFAIDTDAITMKHLSDDDVNYSDNP
                     DVLVFAENGHEMKALRYVDELNEKGINAQFSVLETLEETEQFAKSRGIAKVESI"
     gene            1274..1903
                     /gene="hisG"
                     /locus_tag="PLS64_05565"
     CDS             1274..1903
                     /gene="hisG"
                     /locus_tag="PLS64_05565"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRL43331.1"
                     /translation="MKPLRIALTKGRLEKTTIELLEKIGYNCDEVKNKGRRLILPIGN
                     NDFEVVLAKAADVITYVEHGVCDLGVVGKDTILENGSSFYELLDLGFGKCRFALATKK
                     DSDFYGGYKTKTIASKYPNVTRAFFDDKNMDVRIIKIEGSVELAPLVGLADGIVDIVE
                     TGSTLKANGLEVIEWVCDISARLIANTASLKLRKAEIEELQKKLEAVTK"
     gene            1900..3192
                     /gene="hisD"
                     /locus_tag="PLS64_05570"
     CDS             1900..3192
                     /gene="hisD"
                     /locus_tag="PLS64_05570"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRL43332.1"
                     /translation="MITTVVADGKNEYEFIDLLKKRAQNSDKNVVPIVSEIIENVAEN
                     GDKAVKEYTVKFDGKAPEKVEITSDEIDSLIEKCDKDYLETIKKAAANIADFHKRQVQ
                     QSWLTTKNNGVMMGQRIRGLKRVGIYVPGGTAAYPSSVLMNAVPAKIAGVEEIIMCTP
                     PQKDGTPNPNIIAAAKVAGVDRIFLMGGAQAVAALAYGTESVPKVDKIVGPGNIFVAT
                     AKKLLYGTVDIDMIAGPSEILIIADKSANPKFLAADLMSQAEHDRLASAILLTDCEEL
                     AKETETELEKQMTALSRKDIIRSSVDNFGAVIVCSDMSQAIEFANELAPEHLEVCCEN
                     PMEYVGKLDNAGSVFLGNYSPEPLGDYFAGPNHVLPTSGTARFFSPLSVDSFVKKSSF
                     IYYTEDALRKDAEDVVRFAETEGLTAHANSIKVRFDMK"
     gene            3209..4261
                     /gene="hisC"
                     /locus_tag="PLS64_05575"
     CDS             3209..4261
                     /gene="hisC"
                     /locus_tag="PLS64_05575"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRL43333.1"
                     /translation="MSYELNKKIRELEPYEPISGTYKIRLDANECPENYPDFIKEEIK
                     NAIDTIDFNRYPDPLAEKVVTAFADYYGINPKYVTAGNGSDELIFLIEAAFMEKGDRM
                     LCVAPDFSMYKFYSSICEVECDTFIKSDDLSIDTDALIEKVNRDNIKLLLFSNPCNPT
                     GQGMTAEDARRLVTSVNALVILDEAYMDFWDQSLINEVEKYSNLIIFRTASKAVGSAA
                     LRLGFAVANETISRAVKAVKSPYNVNTFSQVVGSIIYKNKDYLKNRQKTIVRNREKLY
                     NDLLELSALSDDFKVYNSCANFVFVKTSFGKDLWNYLKDNSIVIRFMGDYIRISVGTE
                     EENEELISCIKTYLSR"
     gene            4266..4844
                     /gene="hisB"
                     /locus_tag="PLS64_05580"
     CDS             4266..4844
                     /gene="hisB"
                     /locus_tag="PLS64_05580"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRL43334.1"
                     /translation="MRSAYVERKTKETDIKLKLNLDGGDIKIDTGIGFFDHMLNSFAT
                     HGRFGLEVSVKGDLYVDEHHTVEDTGIVLGKAFAEALSTKESIERFGSFYVPMDEALA
                     FASVDISGRPYLVFDADFPQERCGGYDCSMTVEFMRAFAFNAQITLHLKSVYGDNSHH
                     ITEALYKACAHALRLAVKQNSSNVLLSTKGVL"
     gene            4844..5563
                     /gene="hisA"
                     /locus_tag="PLS64_05585"
     CDS             4844..5563
                     /gene="hisA"
                     /locus_tag="PLS64_05585"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HRL43335.1"
                     /translation="MIILPAIDIKDGNCVRLFKGDYATVQKVAESPYIAAQGFADAGA
                     EWMHMVDLDGAKDAKLVNADLIADVAKASGLSVEVGGGIRDMKAVEYYLSRGINRVIL
                     GSAAVKNQQLVIDAVKEYGERIVVGIDAKDGMVRAEGWIDNSEINYIELAKRMEDVGV
                     KTIVFTDIDQDGTLAGPNLKQLNNLAHEVSCNIIASGGVAVLKDIINLAELDVYGAIC
                     GKAIYTGSLDLKQAIEVTNKI"
     gene            5571..5885
                     /gene="hisI"
                     /locus_tag="PLS64_05590"
     CDS             5571..5885
                     /gene="hisI"
                     /locus_tag="PLS64_05590"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRL43336.1"
                     /translation="MDLDKYFVKSELIPAIIQEKSTGEVLMLAYMNRESMQKTFETGY
                     TWFWSRSRQELWNKGATSGHLQKVIDIYADCDDDTLLITVEQTGAACHTGNHSCFFKK
                     LK"
     gene            5899..6231
                     /gene="hisE"
                     /locus_tag="PLS64_05595"
     CDS             5899..6231
                     /gene="hisE"
                     /locus_tag="PLS64_05595"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HRL43337.1"
                     /translation="MNDREVLEALYATVENRRDNPKEGSYTCYLLDKGIDKILKKVGE
                     ECSETIIAAKNDDNSETVYEIADLIYHLTVMMVKLGIPLEDVLAELDKRSEKTGNLKK
                     FHVVDKNS"
     gene            complement(6299..6538)
                     /locus_tag="PLS64_05600"
     CDS             complement(6299..6538)
                     /locus_tag="PLS64_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43338.1"
                     /translation="MFSVPVSVKNKKEMIDMFETLAHFPDTVMMLRNGDNTVDAHSLM
                     GFFAIDEKIPIELLLESEPDDNLKQAISKFAFVNA"
     gene            6714..7949
                     /gene="mtaB"
                     /locus_tag="PLS64_05605"
     CDS             6714..7949
                     /gene="mtaB"
                     /locus_tag="PLS64_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HRL43339.1"
                     /translation="MREDMLKNGFLLSDDRDRADITIVNSCTVTSVSDAKNRKILNRV
                     RRENPDGIVILTGCMPQAFPEESENFSNCDIVLGNAKRAELVPSIYKFLEEKQKFVHI
                     TEHIGKGEKYENLSVSSLGEHTRAFIKIEDGCNRFCSYCIIPYARGRVRSKSLEDLKA
                     ELIQVAANGYREVVLVGINLSAYGLDEDFDLADAVECACSVDGIDRVRLGSIEPERMD
                     KEMIARLAAQPKFCPQFHLSLQSGCDNTLKAMNRHYNCAEYAEIVTNLRNAFENSSMT
                     TDVMVGFAGETEEDFNASLKFVKSIGFAKVHIFPYSRRKGTVADKAPNQIDPQIKEQR
                     AKIMEQVCDETRREFLKEQVGLCESVLVERLRHGYLEGYTKNYTPVHINSEDTSLCGK
                     IIDVKITKSFDDYCDAELV"
     gene            complement(7941..>8138)
                     /locus_tag="PLS64_05610"
     CDS             complement(7941..>8138)
                     /locus_tag="PLS64_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43340.1"
                     /translation="GLGGYKAGNGEYDEETWLLSDSILADEYDILRNNKSVRGFMLYS
                     YNSLEDDTAKKEINNLINALN"
BASE COUNT         2611 a         1562 c         1732 g         2233 t
ORIGIN      
        1 ttcatctgaa tagtgttgac aaatcaaatt tagtgtgtta atatattacc gtattaacac
       61 actaaagcgt tgagaggtaa ttatgaaaaa gaatttaaaa atgactcccg agggaacaaa
      121 ggactttctg ttcgctgaat gttccgctat ggattatatc tgcacaagca ttgaaaaggt
      181 atttgtcaaa ggcggattta aacgggtaat aactcctgct cttgaatttt atgatgtgtt
      241 ctcaattccg tcaagcggaa tctcgcagga atcaatgttc aagacaaccg ataacaaagg
      301 cagacttctt gttgcccgtc ctgactccac tctgccgatt gcaagaatgg tttctacaag
      361 acttaaaaac aatacacttc ctgttcgact ttactacaag caggcagtat atcgcaacaa
      421 tccgtcactt gcaggaagaa gaaacgaatt tcttcagatg ggtgttgaac ttctcggggc
      481 aaaaggaaag agagccgacc ttgaaattct cacctcggca ataaaaagca tttcagccgt
      541 tgccgatgat ttcagaattg aactcggaca cgccgaagta ttcaatgctc tgtcaaaaga
      601 actcgatatt gataacgatt acaaagaaaa aatcagggta tcaatcgaaa gtaaaaacta
      661 ctcggcgctt aacaatcttc ttgacaaact caagccttgc aagactgttt ccgcaatccg
      721 ttcgctcccg tcacttttcg gcggtgaaga agtgtttgat gaggcatatg acatctgcaa
      781 aggtacaggc gccgaaaacg ctcttgatta tctccacaca atctataaag aactttccgt
      841 gcttgaactc ggcaacaagc ttatcatcga cctcggactt gttcagagaa acgattacta
      901 tacgggaatc gtattttcag ggtatattaa cggaatcggt gacgctgtta tttcgggagg
      961 aagatatgac gatcttctca gcgaatttga cgctcctatg ggtgcggcag gctttgcgat
     1021 tgatacagac gcaattacaa tgaaacatct ttccgatgat gatgtaaatt attcagataa
     1081 tcccgatgtt ctcgtttttg cagaaaacgg acatgaaatg aaggctctca gatatgttga
     1141 cgagcttaac gaaaagggaa taaatgcaca gttcagcgta cttgaaactc ttgaagaaac
     1201 agaacagttt gcaaaatcaa gaggaattgc aaaagttgaa agtatataat caagctgtcg
     1261 gaaaggatta aatatgaaac cattaagaat tgctctgaca aagggcagac ttgaaaagac
     1321 cacaattgaa cttcttgaaa aaatcggata taattgtgat gaagtaaaaa acaagggaag
     1381 aaggcttatt cttccgatcg gcaataatga ttttgaagtt gtgcttgcga aagccgcaga
     1441 tgtaattaca tatgttgagc acggtgtgtg tgaccttggt gtcgttggca aggatacaat
     1501 tcttgaaaac ggttcatctt tttatgaact tctcgactta ggattcggta agtgccgttt
     1561 tgctcttgct acaaaaaagg actccgattt ttacggcggt tacaaaacaa agacaattgc
     1621 gtcaaagtat cccaatgtaa ccagggcatt ttttgacgat aaaaatatgg atgtaagaat
     1681 cattaagatt gagggctcgg ttgaactcgc tcctcttgta ggtcttgcag acggtattgt
     1741 agatatagta gaaacaggtt caacgctcaa ggctaacgga cttgaagtaa ttgaatgggt
     1801 atgtgatatt tcagcaagac ttattgccaa tacggcaagt ctaaaattgc gcaaggctga
     1861 aattgaagaa ttgcagaaga aactggaggc tgtcacaaaa tgattacaac tgttgtagca
     1921 gacggaaaaa acgaatatga atttattgat ttgctcaaaa agagagcgca gaattcagat
     1981 aaaaatgtag ttccgattgt ttcggaaatc atcgaaaatg ttgcagaaaa cggtgataaa
     2041 gccgtaaagg aatataccgt taaatttgac ggaaaagcac ctgaaaaggt agaaattact
     2101 tccgatgaaa tcgacagcct tattgaaaaa tgtgataagg attatctcga aacaatcaaa
     2161 aaggcagccg caaatattgc agacttccat aaaagacagg ttcagcaaag ctggctcaca
     2221 acaaagaata acggcgtaat gatggggcag agaatcagag gccttaaaag agtaggtatc
     2281 tatgttccgg gtggcactgc agcatatccc tcatcagttc ttatgaacgc agttcccgct
     2341 aaaattgcag gtgttgagga aattattatg tgtacacctc ctcagaagga cggtacaccg
     2401 aatccgaata tcatcgccgc cgcaaaggta gcaggtgttg acagaatctt ccttatgggc
     2461 ggtgcacagg cagtagccgc acttgcatac ggtacagaat ccgtaccaaa ggttgacaaa
     2521 atcgtaggcc ccggcaacat ctttgttgca acagcaaaaa aacttcttta cggaacagtt
     2581 gacattgata tgattgccgg tccgagtgaa atactcatca ttgccgataa gtctgcaaat
     2641 ccaaaattcc ttgccgcaga ccttatgagt caggcagagc atgacaggct tgcatctgca
     2701 attctgctta ccgactgcga agagcttgcc aaagaaactg aaacagaact tgaaaagcag
     2761 atgaccgctc tttcaagaaa agatattatc cgttcatcgg ttgacaattt cggtgcagtt
     2821 attgtatgca gtgatatgtc acaggcgatc gaatttgcta acgagcttgc tcccgaacat
     2881 cttgaggttt gttgtgaaaa cccaatggag tatgtcggca agcttgataa tgccggctct
     2941 gtatttctcg gcaactacag tcccgagcct ctgggcgact attttgcagg tcctaaccat
     3001 gttcttccga caagcggcac ggcaagattt ttctcacctc tttcggtaga tagctttgta
     3061 aagaaatcaa gctttattta ctatacggaa gacgctttga gaaaagatgc cgaagacgta
     3121 gttcgctttg cagaaacaga aggacttaca gcacacgcaa attcaattaa agtgcgtttt
     3181 gatatgaaat aagaatggaa gttatgcaat gtcttatgaa ttaaacaaaa aaatccgtga
     3241 acttgaaccg tatgaaccaa tcagcggtac atataaaatt cgacttgatg caaacgaatg
     3301 tcccgaaaat tatcctgatt tcatcaaaga agaaatcaaa aatgcaattg atacaattga
     3361 tttcaaccgc tatcccgatc cgcttgcgga aaaggttgtt acggcttttg cggattatta
     3421 cggaatcaat ccaaaatatg taaccgcagg taacggctca gatgaactta tctttttgat
     3481 tgaagccgca tttatggaaa aaggggacag gatgctttgc gttgctcccg atttttcaat
     3541 gtacaaattc tattcatcaa tctgtgaggt tgaatgtgac acatttatca aatccgacga
     3601 cctttcaatt gatacggacg cacttattga aaaggtaaat cgtgacaata tcaaactttt
     3661 actcttttca aacccatgca atccgacagg gcagggtatg accgccgaag atgcacgcag
     3721 gcttgttaca agcgtaaatg cacttgtaat tcttgacgaa gcatatatgg atttctggga
     3781 tcagagtctt ataaatgagg ttgaaaagta ttcaaatctc attattttca gaaccgcatc
     3841 aaaggcagta ggctcagcgg cactcagact cggttttgcc gttgcaaatg aaacaatttc
     3901 aagagctgta aaagcggtaa aatctccgta caatgtgaac acattttctc aggttgtcgg
     3961 cagtataata tataaaaata aagattatct caaaaacaga caaaaaacaa ttgtcagaaa
     4021 cagagaaaag ctgtataatg acttattgga gttgtccgct ctttccgatg attttaaggt
     4081 ttacaacagt tgtgcaaact ttgtttttgt taaaacatca ttcggcaaag acttgtggaa
     4141 ctatctcaag gacaattcaa ttgtaatccg ttttatgggc gattacatca gaatttccgt
     4201 tggcacagaa gaagaaaacg aagaactgat aagttgcatt aaaacttatt tatcgaggtg
     4261 attatatgcg cagtgcatat gttgagcgta aaacgaagga aaccgacata aagctaaaac
     4321 ttaatcttga cggcggcgat attaaaattg atacaggcat aggctttttt gaccatatgc
     4381 tcaattcgtt tgccactcac ggcagattcg gacttgaggt ttcggtaaaa ggcgaccttt
     4441 atgttgacga acaccacacc gttgaagaca caggcatagt tctcggcaaa gcatttgcag
     4501 aggctctttc aaccaaagag tcaattgaga gatttggcag tttttatgtt cctatggacg
     4561 aagcgcttgc gtttgcatct gttgacatca gcggcagacc ttatcttgta ttcgacgctg
     4621 atttcccgca ggaaagatgc ggcggctacg actgttcaat gacagtagaa tttatgcgtg
     4681 catttgcatt taacgcacaa atcacacttc acctcaaatc tgtttacggt gacaactctc
     4741 accatatcac agaggctttg tacaaggctt gcgcacacgc actccgtctt gcagtaaagc
     4801 aaaattcttc aaatgtactt ctttctacta aaggagtgtt ataatgatta ttttacccgc
     4861 tattgacata aaagacggca attgtgtccg tcttttcaag ggtgattatg caaccgttca
     4921 gaaggtagca gagtccccgt atattgccgc tcagggcttt gctgacgcag gtgcagagtg
     4981 gatgcatatg gttgaccttg acggtgcaaa agacgcaaag cttgttaatg ccgatcttat
     5041 tgcggatgtt gcaaaagcct cgggactttc tgttgaggtc ggcggaggaa tccgtgatat
     5101 gaaagcggtt gaatattatc tttcaagagg aataaaccgt gttattctcg gttcagccgc
     5161 agttaaaaac cagcagcttg ttattgatgc cgtaaaagaa tacggtgaga gaattgtagt
     5221 tggcattgat gcaaaggacg gcatggttcg tgcagaaggt tggattgaca attcggaaat
     5281 caactacatt gagcttgcaa agcgtatgga agatgtcgga gtaaagacta ttgtgttcac
     5341 agacattgat caggacggta ctcttgcagg tcctaacctt aaacagctta ataaccttgc
     5401 tcacgaagtc agctgtaata ttatcgcaag cggcggtgtt gcagttctca aagatatcat
     5461 taatcttgca gaactcgatg tctacggtgc aatctgcggt aaggctatct acacaggttc
     5521 gctcgatttg aaacaggcga tcgaagtaac aaataaaatc tgaggtaatt atggatcttg
     5581 ataaatattt tgtaaaatcc gaacttattc ccgcaattat tcaggaaaag tcaacgggtg
     5641 aggtacttat gcttgcatat atgaaccgtg aaagtatgca aaaaactttt gaaaccggtt
     5701 acacatggtt ctggtcaaga agccgtcagg agctttggaa caagggtgca acatcaggtc
     5761 atttacaaaa ggtaattgat atatatgccg actgcgacga cgatacattg ctcatcacag
     5821 ttgaacagac aggtgcggcc tgccacacgg gaaatcattc ctgctttttt aaaaaattaa
     5881 aatgaggaga attttaatat gaacgacaga gaagttcttg aagcacttta tgctacagta
     5941 gaaaacagac gagataatcc aaaagagggt tcatacactt gctaccttct tgataaagga
     6001 attgacaaga ttcttaaaaa agtaggcgag gagtgcagtg aaacaatcat tgcggcaaaa
     6061 aatgatgata acagcgaaac tgtttatgaa attgcagacc ttatctatca tcttactgta
     6121 atgatggtaa agctcggcat tccgcttgaa gatgttcttg ccgaacttga caagagaagc
     6181 gaaaagacag gaaaccttaa aaagttccat gttgttgaca aaaattcata actaaaagca
     6241 caaacaaaaa ggccctatga ctttggtcat agggcttaac agtatgtata tattattttt
     6301 atgcattaac aaaagcaaat ttagaaatcg cctgtttgag attatcatca ggttctgatt
     6361 caagaagaag ttcaatcgga attttttcgt caattgcaaa aaatcccatc aacgaatgag
     6421 catcaacagt attatcgccg tttcttagca tcattactgt atccggaaag tgagcaagag
     6481 tttcaaacat atcgatcatt tcttttttat ttttaacgga aaccggtaca gaaaacaaga
     6541 tctcacctcc attgttcatc ttgtctatat tttatcatta atttttgtct aaatcaacaa
     6601 cgaattaatg aatttttgca atttcaacta tttacccaaa aaaggatggt tagtgaatga
     6661 attttaatat aattacactc ggctgcaagg ttaattagta tgaatcacag gcaatgcgtg
     6721 aagatatgct gaagaacgga tttcttctct cggatgacag ggacagggca gacataacaa
     6781 tcgttaacag ctgtacggta acatccgtaa gtgacgcaaa aaaccgcaag attctcaacc
     6841 gtgtccgccg tgaaaatcct gacggaattg ttattctcac cggctgtatg ccacaggctt
     6901 tccccgaaga aagcgagaac ttttcaaatt gcgatatcgt actcggtaac gcaaagagag
     6961 ccgaactcgt tccttcaatt tacaagtttc tcgaagaaaa acagaaattt gttcatataa
     7021 cagagcatat cggaaaaggc gaaaaatacg aaaacctttc cgtatcgtca ctcggagagc
     7081 atacaagagc tttcattaaa attgaggacg gttgcaacag attttgttct tactgcatta
     7141 ttccgtatgc aaggggcaga gtgcgttcaa aatctcttga agatttaaag gcagagctta
     7201 ttcaggttgc cgcaaacggt taccgtgagg ttgttctcgt tggtattaac ctttccgcct
     7261 acggtcttga cgaagatttt gatcttgctg atgctgtaga atgtgcttgt tccgttgacg
     7321 gaatcgaccg tgtaagactc gggtcaatcg aaccggaaag aatggataaa gaaatgattg
     7381 cccgtcttgc cgcacagcca aagttctgtc cgcagtttca tctttcacta caaagcggtt
     7441 gcgataacac actcaaagca atgaaccgtc actacaattg tgcagaatac gctgaaattg
     7501 ttacaaatct cagaaacgct tttgaaaaca gttctatgac aaccgatgta atggttggat
     7561 ttgcaggtga aacagaggaa gatttcaacg catctcttaa atttgtaaag tcaatcggct
     7621 ttgcaaaggt gcatatcttc ccgtattcac gccgtaaagg tactgttgcc gataaagcac
     7681 caaatcaaat tgatccgcaa attaaagagc agagagccaa aattatggag caggtctgcg
     7741 atgaaaccag aagggaattt cttaaagaac aggttggcct ttgtgaatct gttctcgttg
     7801 aaagacttcg tcacggttat cttgagggtt acacaaagaa ctacacgcct gttcatatta
     7861 attccgaaga tacaagtctc tgcggtaaaa ttattgatgt gaaaatcaca aaatcatttg
     7921 atgactactg cgatgctgaa ttagtttaaa gcgttaatca gattattaat ctcttttttt
     7981 gcagtatcat cctcaaggct attatagctg taaagcataa agcccctgac agatttgttg
     8041 ttacgcaaaa tatcgtattc atcggcaaga atactgtcac ttaaaagcca tgtttcttcg
     8101 tcatattcgc cgttacctgc cttataaccg ccaagacc
//
LOCUS       DAOMVE010000078         8099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2316_length_8099_cov_3.209846, whole genome shotgun sequence.
ACCESSION   DAOMVE010000078 DAOMVE010000000
VERSION     DAOMVE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2316_length_8099_cov_3.209846"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..674
                     /locus_tag="PLS64_05615"
     CDS             138..674
                     /locus_tag="PLS64_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HRL43341.1"
                     /translation="MNIWHDISPKRITKDKFYAVIEISKGGKNKYELDKETGMLKLDR
                     VLFTSTHYPANYGFIPRTFADDGDPLDVLVLCSETIQPMTLVECKPIGVLNMVDNDSC
                     DEKIIAVPVNDPNYNCYSDISELPKHYFEEIQHFFQVYKTLEADKVTSVTETDGVDKA
                     KEIIEKAITSYIRDFQMA"
     gene            complement(802..1008)
                     /locus_tag="PLS64_05620"
     CDS             complement(802..1008)
                     /locus_tag="PLS64_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43342.1"
                     /translation="MKKSLFGKNIPVNCSYCEYNGIENDIMFCKKSKQVKDGKCRSFK
                     YDPLLRMPNVTVFKTDFSAKDFKL"
     gene            1143..1979
                     /gene="pgeF"
                     /locus_tag="PLS64_05625"
     CDS             1143..1979
                     /gene="pgeF"
                     /locus_tag="PLS64_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HRL43343.1"
                     /translation="MKFFSKTMKLNNIDTVPYLTYNSLSEISFINHAFTTRLGGVSTG
                     EFTSMNMAFNRGDNPDSVTENYKRFCKSAGFDYESLVASAQDHHTVVRAVTSAEKGIG
                     IYKPRDMESVDALITNEKGVTLVTYYADCTPLFFVDTKNKAIGLAHAGWRGTVGRIGE
                     KVVEKMTELYGTNPADIKAAIGPAISVCCYEVDYPCAEHFLNLADLDSSKFVFEKGNG
                     KYMVDLLETNRQILTASGVKNENITVSDVCTNCNSDLLWSHRATKGHRGTMSAFMCIK
                     ED"
     gene            complement(2022..2987)
                     /locus_tag="PLS64_05630"
     CDS             complement(2022..2987)
                     /locus_tag="PLS64_05630"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRL43344.1"
                     /translation="MRTLEIKKNDANQRLDKFLQKRFKTLPKSLMYKYIRKKCIKVNG
                     KKCDIQTMLKEGDVLTFYIKDEFFEASEQKSYEFMKAPKTFDIVYEDENLILIDKKPG
                     IIVHPDKNYHFDSLVARVQHYLYDKGEYNPDEEKAFAPALVNRIDRNTGGIVIAAKNA
                     ESLRILNAKMKTRELEKYYLCLLYGKPKRDSDTLHGYMTKNESKNKVTVFKNEVEGSK
                     EIKTKYEILDFNGKFSLAEVELLTGRTHQIRAHMASIGHPLVGDTKYGKGKKAPDSIK
                     YQALYSYKLKFNFETDAGILDYLNGKEFEVNDVWFAKNGKINLDR"
     gene            3105..3248
                     /locus_tag="PLS64_05635"
     CDS             3105..3248
                     /locus_tag="PLS64_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43345.1"
                     /translation="MNCYTQRIKSAVCFGLGLLCASLLPSEWVLVIAAGALVVVSICC
                     VRR"
     gene            3251..3340
                     /gene="spoVM"
                     /locus_tag="PLS64_05640"
     CDS             3251..3340
                     /gene="spoVM"
                     /locus_tag="PLS64_05640"
                     /inference="COORDINATES: protein motif:HMM:NF033436.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein SpoVM"
                     /protein_id="HRL43346.1"
                     /translation="MKVVLIEKPKFISAILRKIYGIKKETKPE"
     gene            3538..5061
                     /locus_tag="PLS64_05645"
     CDS             3538..5061
                     /locus_tag="PLS64_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HRL43347.1"
                     /translation="MEFNQENRSFCTPLAVLDTVAEQLADVDLTLPDYCPDIEKILKC
                     TLTPKIQSRTLSGGQLQIDGFCTVNVMYVESIKKSVRCCEQSVNFSQNFSVKDAPDNP
                     VIITKTKPEYINCRALSPRRLVMHGAFSLYAKVFSKTQTSIFTPSDNIETDRKTVTCA
                     DLKSFCQEQFSVCEEISVGEKPQIESVLRSDVSVGVIDAKAVTGKLMLNGELNLKLLY
                     LSNAESGEVEKLDYLLPFNKILDCDGIDENTINCVSCDVMSYDIRLKNDMLSEKPSVI
                     LEVKLCVTEEGYITREEEIVCDAYSTEFSSLPEFEQLKITGEVVPINEPHMEKMSVKI
                     DDGKISRILDIFTEQITAEASHDDRGMVISGKINICILALNGEDMPVFIERSADYEHI
                     IPQTDCTCAKIFGTRTASISYRLAEDDVIEIRCELKICGAVFSENRVNTVKNVNVFED
                     KPIPPENCALTLYFADKGEKLWDIAKSHNTKLDLLLSENSAENQVLDAPKMLLIPRI"
     gene            5075..6553
                     /gene="spoIVA"
                     /locus_tag="PLS64_05650"
     CDS             5075..6553
                     /gene="spoIVA"
                     /locus_tag="PLS64_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HRL43348.1"
                     /translation="MEERNVYQDISRRTDGDVYIGVVGPVRTGKSTFIKRFMDSLVLP
                     NIRDEAVYRRTVDELPQSAAGRTIMTTEPKFIPEESVEINLGGNTTFNVRMIDCVGYI
                     VNSAIGYSEEDEPRMVKTPWSNEAIPFDDAAEIGTKKVITDHSTIGLVITTDGTISDI
                     PREDYIESEKRVINELKAINKPFIILLNSVDPTADETIRLSNEMTAEYKVPVIPVSCM
                     ELDETEIKRILAQLLFEFPIREIKVDIPKWVVKLDKDHWLKSVVFDSIKKNAAKIEKI
                     RQIQQIISDIEQCEYITSAKVTSLDLGKGYAEMAVSIESELFYKVISEETGLEITEEY
                     ELLNCIKTLSQKQKEFDKIAQAYEQVQETGYGIVMPTIDELTLDEPQIIKQSGKYGIK
                     LRASAPSIHMMKIFTQTEVTPIVGSEQQSEELVSYLLREFDENPEKIWESNIFGKSVH
                     ELVNEGLHNKLYRMPADARRRIGETVEKIINDGCNGLICIIL"
     gene            6725..7147
                     /locus_tag="PLS64_05655"
     CDS             6725..7147
                     /locus_tag="PLS64_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRL43349.1"
                     /translation="MNLSDVALLAKEELSLAEKGIASAKVVLTGFVVVFAMLILLIFI
                     IKIYGAIIQKAQNAGKKSKEKKAETVAETPAPVVAAPVAPAQTDGVSDEVVAVISAAV
                     ATMYGSSEKARIKSIKKSSDGGRSAWAKAGVLDNTRPF"
     gene            7179..>8099
                     /locus_tag="PLS64_05660"
     CDS             7179..>8099
                     /locus_tag="PLS64_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRL43350.1"
                     /translation="MEILQGFVDAVVGIFEDSGLLTLNWQNYVMIGISIVLLYLAIKK
                     QYEPLLLLPIAFGMLLVNLYPSIMAPPTTQLLTEAQCTAQNVAVSGHAKTVIDGVTYI
                     ENPTYGGLLYYLYQGVKLGIYPPLIFLGIGCMTDFGPLISNPKSLILGAAAQIGIFLT
                     FTGAIFLGFTEAEAGAIGIIGGADGPTAIYVTTKLAPHLLGSIAIAAYSYMALVPIIQ
                     PPIMKALTTKKERSVVMEQLRPVSKLEKIMFPVIVVIIIAIFLPDAAPLVGMLMLGNL
                     FKESGVVERLSKTAQNELMNIITIFLGTTVG"
BASE COUNT         2471 a         1600 c         1681 g         2347 t
ORIGIN      
        1 tttgaattat tttgattgaa attacaaaaa ttccttgaca aagtttgaca gatttcttat
       61 aattaaacca taacataatg tttcacaggg tattacagga tatataaaaa ataaggttaa
      121 gaagggtgac agaaattatg aacatttggc acgacatttc tccgaagaga attacaaaag
      181 acaagtttta tgcagtaatc gaaatttcaa agggcggcaa aaacaagtat gagcttgaca
      241 aagaaacagg tatgctcaag cttgaccgtg tgctttttac atcaactcac tatcctgcca
      301 actacggctt tattccgaga acctttgcag atgacggcga tccgcttgat gttcttgttt
      361 tgtgcagtga aacaattcag ccgatgacgc tcgttgaatg caagcctatc ggcgttctca
      421 atatggttga taacgacagc tgtgacgaaa aaattattgc agttcccgtt aacgatccca
      481 actacaattg ctacagcgac atcagcgaac ttccaaaaca ctattttgaa gaaattcagc
      541 actttttcca ggtttacaaa acacttgagg ctgacaaggt tacttcggta accgaaactg
      601 acggtgttga caaggcaaag gaaatcattg aaaaggctat cacaagctat attcgtgatt
      661 ttcagatggc gtaaatttat aaagactgca aaactaaaag ctttaccaaa aacggtaaag
      721 cttttttctt ttgaatgtgt aatttaattc tgaaaaggaa tattaacaaa attcattcct
      781 gtataataaa aatacggcgg attaaagttt aaaatccttt gccgaaaaat ctgtcttaaa
      841 aaccgtgaca ttcggcatac gcaacaacgg gtcatacttg aacgatctgc attttccgtc
      901 cttgacctgc tttgactttt tgcagaacat aatatcattt tcaattccgt tgtattcgca
      961 atatgaacag ttgacaggaa tattcttgcc aaacaaactt tttttcataa tcaacacctc
     1021 tgaatgaata tagtatatca ttaactcagc cgtgtttcaa gcataatatt ttgtcggaaa
     1081 atattgacaa agcaattaat cggttaaaaa taatcttatt ataaaatatg gaaggaacac
     1141 ttatgaaatt tttttcaaaa acaatgaagc tcaacaacat tgatacggtt ccgtatctta
     1201 catataattc tttatcagaa attagtttca tcaatcacgc attcacaacc cgactcggcg
     1261 gtgtgtcaac gggtgaattt acatcaatga atatggcatt caacagaggt gacaatcccg
     1321 acagcgtaac cgaaaattac aaaagattct gcaaaagtgc aggctttgac tatgaaagtc
     1381 ttgtagcctc ggcacaggat caccacacgg ttgtaagagc ggtaacatct gccgaaaagg
     1441 gcataggcat ctacaagccc cgtgatatgg agagcgttga cgctttgatt acaaatgaaa
     1501 agggagtaac cctcgtaacc tactatgccg actgcacacc gctgtttttt gtcgatacaa
     1561 agaataaggc aatcggactt gcccatgccg gctggagggg aactgtcggc agaatagggg
     1621 aaaaggttgt tgagaaaatg actgagcttt acggcacaaa tcctgccgat atcaaagctg
     1681 caatcggtcc agcaatttca gtttgttgct atgaggttga ctacccatgt gcagaacatt
     1741 ttctgaatct tgcagacctt gacagctcaa agttcgtttt tgaaaaagga aacggaaaat
     1801 atatggttga ccttttagaa accaaccgtc agattcttac tgcttcaggt gtaaagaatg
     1861 aaaacataac agtttcggat gtctgcacca attgcaacag cgacctctta tggagtcacc
     1921 gtgccaccaa aggtcacaga ggcacaatga gtgcgtttat gtgtattaaa gaagattaaa
     1981 atttttgttt ttgcaaagca aaaggcggtt attgaccgcc tttatctatc tagatttatc
     2041 tttccgtttt ttgcaaacca tacatcgttt acctcaaatt ctttgccgtt aaggtaatca
     2101 agaatgcctg catcggtttc aaaattgaat ttcagcttat agctgtaaag cgcctgatac
     2161 ttaatgctgt cgggtgcttt tttgcctttg ccgtattttg tatcgcccac aagcggatgt
     2221 ccgattgatg ccatatgcgc tcttatctga tgagttctgc ccgtcagcag ttcaacctcg
     2281 gcaaggctga atttgccgtt gaagtcaaga atttcatatt ttgtcttgat ttcttttgaa
     2341 ccctcaactt catttttgaa aacagtaacc ttgtttttac tttcattctt ggtcatatag
     2401 ccgtgaagtg tgtcgctgtc cctttttggc tttccgtata aaaggcagag atagtatttt
     2461 tcaagctctc ttgttttcat ttttgcgttt aggattctta gactttcggc atttttagcc
     2521 gcaataacaa ttccgcccgt gtttctgtca attctgttta caagtgcagg tgcaaaggct
     2581 ttttcttcat cgggattata ttcacctttg tcatacaggt agtgctgaac tcttgcaaca
     2641 agtgaatcaa agtgataatt cttgtcgggg tgtacgatta tacccggctt tttatctata
     2701 agtataaggt tttcatcctc ataaacaatg tcaaaggttt tcggtgcctt catgaattca
     2761 tagctttttt gctccgatgc ctcaaaaaat tcgtccttaa tataaaaggt aagcacatcg
     2821 ccctctttga gcatggtctg aatatcacac tttttgccgt ttaccttaat acactttttc
     2881 cttatatatt tgtacataag cgactttggc agagttttga atcttttctg taaaaattta
     2941 tcgagtcgct gatttgcgtc attcttttta atttcaagtg ttctcatagt ctgtaaaatt
     3001 ctccgtaatt gttttctcag tttatcttat atcaatttta aaaataattc aaccgctttt
     3061 gcacataatt tgattttgtg tatattctga tatagggtga tacagtgaat tgttacacac
     3121 agagaataaa atctgcagta tgctttggac tgggactgct gtgtgcatca cttctgccga
     3181 gtgaatgggt actcgtcatt gcggcaggcg cactcgtagt tgtcagtatt tgttgtgttc
     3241 ggaggtaatt atgaaagttg tacttattga gaaaccaaaa ttcatatcgg caattttgag
     3301 aaaaatttac ggaatcaaaa aggaaaccaa gccggaataa tagtcatatg tttggacact
     3361 gttccaaaat cttgctattg gtaatagttt acaataaatt cgagccaaag atgtgcatag
     3421 ttacaaaaaa ctttgcacat cttatttttt aaaataaaat tatgattaat cttacgggta
     3481 atatttttcc gcactcccta atataattga actataaaac ataaaggaga gaaaaatatg
     3541 gagtttaatc aggaaaaccg ctcgttttgc acgccgttgg ctgtgcttga cacggttgcc
     3601 gaacagcttg cagatgttga cctcactctg cccgattatt gtccggatat agaaaaaatt
     3661 ctgaaatgta ccctcacacc caaaattcag agcagaaccc tttcgggagg tcagcttcag
     3721 attgacggat tttgtacggt aaatgtgatg tatgtggaaa gcataaagaa aagcgtgcgc
     3781 tgttgcgaac aaagcgtgaa tttttcacag aacttttcgg taaaagacgc tcccgacaat
     3841 ccggttatca tcacaaaaac caagccggaa tacataaact gcagggcatt aagcccccgc
     3901 agacttgtta tgcacggagc gttttcgttg tatgcaaagg tattttcaaa aactcagacg
     3961 agcattttca cgccttccga taatatcgaa accgaccgaa agaccgttac ctgtgccgat
     4021 cttaaatcgt tctgtcagga gcagttttct gtttgcgaag aaatttcggt aggcgaaaaa
     4081 ccgcagattg aatccgttct ccgcagcgat gtaagcgtcg gagtgattga cgctaaggct
     4141 gtaacgggaa agcttatgct caacggtgaa cttaatctca agcttttgta cctttcaaac
     4201 gcagagagcg gagaagttga aaagcttgac tatctgttgc cgttcaacaa aattttagat
     4261 tgtgacggaa ttgacgaaaa cacaatcaac tgtgtttcct gcgatgttat gtcgtatgat
     4321 atcagactga aaaacgatat gctttccgaa aagccgtctg tgatacttga agttaagctc
     4381 tgcgttacgg aggagggcta tataacccgt gaggaggaaa ttgtatgtga cgcctactca
     4441 acagaatttt cctcgttgcc ggagtttgaa caattaaaaa ttacgggcga ggttgttccg
     4501 attaatgaac ctcatatgga aaaaatgtcc gttaagattg atgacggaaa aatctcacga
     4561 attctcgata ttttcacaga acaaataact gccgaggctt cacacgatga cagaggtatg
     4621 gttataagcg gaaaaatcaa tatatgcata ttggcgctta acggggaaga tatgcctgtg
     4681 tttattgagc gttcagccga ttatgagcat attattccgc agacagactg cacctgtgcc
     4741 aaaattttcg gtacaaggac agcaagtata agctacagac ttgccgagga tgatgtcata
     4801 gaaattcgtt gtgagctgaa aatctgcggt gctgtttttt cggaaaacag agtaaacacc
     4861 gtaaaaaatg taaatgtttt tgaggataaa cctatacctc ccgaaaattg tgcgctgaca
     4921 ttatactttg ccgacaaggg agaaaagctc tgggatattg ctaaatccca caacacaaag
     4981 cttgatttgc tgctttcgga aaattcggct gaaaatcagg tgctcgacgc acctaaaatg
     5041 ctgttgattc cgagaatata gggaggtata aaaaatggaa gagagaaatg tttatcagga
     5101 tatttcccga agaaccgacg gcgatgtcta tatcggagta gtcggtcccg tcagaacggg
     5161 aaaatctaca tttataaaaa ggtttatgga ttcgcttgtt ctgcccaata tccgtgacga
     5221 ggcagtttac aggcgtaccg ttgacgagct tcctcaatct gcggcaggaa gaaccataat
     5281 gaccacagaa cccaaattta ttcccgagga atccgttgaa atcaatctcg gaggcaacac
     5341 aaccttcaat gtgagaatga ttgactgcgt aggctatatt gtaaacagcg caatcggtta
     5401 cagcgaagag gacgaaccga gaatggtaaa aactccgtgg tcaaacgagg cgattccttt
     5461 tgatgacgcc gcagaaatag gcactaaaaa ggtaattacc gaccattcaa caatcggtct
     5521 tgttatcact actgacggaa caatcagcga tattccgaga gaagattata ttgaaagtga
     5581 aaaaagagta attaatgagc tgaaggcaat caataaaccg ttcattatac ttctcaattc
     5641 agttgaccct acggcagatg aaacaattcg cctgtcaaac gaaatgaccg ccgaatacaa
     5701 ggttcccgta attcccgtta gctgtatgga gcttgacgaa accgaaatca aaaggatact
     5761 tgcacagctt ttgtttgaat ttccaatccg tgaaatcaag gttgacattc cgaaatgggt
     5821 tgtaaagctc gataaagacc attggctgaa atcggttgta tttgattcaa taaagaaaaa
     5881 tgccgcaaaa attgagaaaa tcaggcaaat tcagcagata atctctgata ttgagcaatg
     5941 cgagtacatc acatctgcaa aggttacgag tcttgacctc ggcaaaggat atgcggagat
     6001 ggccgtatct atcgaaagcg aacttttcta caaggttatc tcggaagaaa cagggcttga
     6061 gataaccgag gagtacgaac ttctgaactg cataaaaact ctttcgcaaa aacaaaaaga
     6121 attcgacaag attgcacagg cttatgaaca ggttcaggaa acgggctacg gaattgttat
     6181 gccgacaatt gacgaactta cccttgacga accgcagatt attaagcaga gcggaaaata
     6241 cggtatcaag ctgagagcaa gcgcaccgtc aattcatatg atgaagattt ttacgcagac
     6301 ggaggtaact ccgattgtcg gttcggaaca gcagtccgaa gagcttgtgt cttatcttct
     6361 cagagaattt gacgaaaatc cggaaaaaat ctgggagagt aacatattcg gaaaatcagt
     6421 tcatgaactc gttaacgagg gacttcacaa caagctgtac agaatgcctg ccgatgccag
     6481 aaggcggata ggggagacag ttgaaaaaat tatcaatgac ggctgtaacg gattaatatg
     6541 tataattctg taacgacaaa taacgataaa atcgcaaagc tttaattcac aaataaacag
     6601 gctcacaaat aaataaattg gtaatatcgc caaaaaatta ttcgccccat atttgttatt
     6661 gaaaatgttt cgggaataaa gtataatatt cctatatgca aatgacaaat atgggggttg
     6721 ttttatgaat ctttccgatg tagctttatt ggctaaagag gagctttccc ttgccgaaaa
     6781 aggtattgcc tctgcaaaag ttgttcttac aggttttgtt gttgtattcg caatgctgat
     6841 tctcttgatt ttcataatca agatttacgg cgcaatcatt cagaaagctc aaaatgccgg
     6901 caaaaagagc aaagagaaaa aggctgaaac cgtagcagaa actcctgctc ccgttgttgc
     6961 cgctcctgtt gctcctgctc agacagacgg tgtttccgat gaggttgttg ccgtaatttc
     7021 cgcagcagtt gccacaatgt acggctcttc cgaaaaagca agaatcaaga gtattaaaaa
     7081 gtcatcagac ggcggtcgtt ccgcatgggc aaaagccggt gtacttgata atacccgccc
     7141 gttttaaggc aaagttttaa gggaaaggat gttatctaat ggaaatttta caaggctttg
     7201 tagatgccgt tgtcggtatc tttgaagact cgggtctgtt aactcttaac tggcagaact
     7261 atgtaatgat tggtatttca atcgttttgc tttaccttgc aatcaaaaag cagtatgagc
     7321 cgcttttgct ccttcctatc gcattcggta tgctgcttgt taatctttat ccgagcatta
     7381 tggctccgcc tacaacacag cttttgacag aggctcaatg taccgcacag aatgttgcgg
     7441 tgtcgggaca cgccaaaacg gtgattgacg gagtaactta tattgagaat ccgacatacg
     7501 gcggacttct ttattacctc tatcagggtg ttaagctcgg catttatccg cctcttatct
     7561 tcctcggcat cggttgtatg actgacttcg gtccgctcat ctctaacccg aagagcctta
     7621 tcctcggtgc tgccgcacag attggtatct ttttaacatt tacaggtgct attttcctcg
     7681 gatttacaga ggcagaggca ggtgctatcg gtatcatcgg cggtgccgac ggtcctacgg
     7741 ctatttatgt aacaacaaaa cttgctccgc atcttctcgg ttcaatcgca attgccgcat
     7801 attcatatat ggctcttgtg ccgattattc agccgcctat tatgaaggca ctcacaacca
     7861 aaaaagagcg ttccgtagtt atggagcagc ttcgtcctgt ttcaaagctt gagaagatta
     7921 tgttcccggt aattgttgtt attatcatcg caatcttcct tcccgacgca gctccgctcg
     7981 ttggtatgct tatgctcggt aacctcttta aggagtccgg cgttgttgaa agactcagca
     8041 aaacagctca gaacgagctt atgaatatta tcacaatctt cctcggtaca actgtaggt
//
LOCUS       DAOMVE010000079         8066 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2323_length_8066_cov_3.989140, whole genome shotgun sequence.
ACCESSION   DAOMVE010000079 DAOMVE010000000
VERSION     DAOMVE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2323_length_8066_cov_3.989140"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLS64_05665"
     CDS             complement(<1..1443)
                     /locus_tag="PLS64_05665"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HRL43351.1"
                     /translation="MKLSKKLCITAKKSFSLVLALTLMLSICAVSGMSLNVFAATSLD
                     QKIYINLNKNKEWNGFSSVTCRFAQDDGTVLKTEKVSKDPSSGVFEATAPSGATRIEL
                     SSGVNFTLPKTTVAKDFRRIYLYNSNNTYNEAYAYSWVNDTDFNAEWPGVAMTKTSSD
                     SDYYYVDVKSSHKNVIFSNKGETQTSDLGINDSYSADNALYDASKSQWTNPFIKTIDI
                     SGASGDTEFYLTTDGSFKESKYLSVEAPDKQSKATYKKVYVSNDDWKSLTKVYATFDY
                     NDAYEGTVELTKDTKDTKVSGSVVFKGEIPAGALLRFHPNEHNLNGASSATSYPTDSG
                     YDGSGYNDNTATYVKTARGEGWTKFSEIDNVNYGAVVENSFSDNPDIVGVDATYFDYW
                     SDMEQEKGYLQCQGKNNDGDIENYWYQFDNFNSYISNIALDHKSDWKYPLYFGNMYKG
                     GEHYKEFTDHVAGLTNINDFNDNYYYAVNNS"
     gene            complement(2097..4964)
                     /gene="secA"
                     /locus_tag="PLS64_05670"
     CDS             complement(2097..4964)
                     /gene="secA"
                     /locus_tag="PLS64_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRL43352.1"
                     /translation="MNFLKAMFGNYSKREVKRVQPICDKVLALEDKYAAMSESELKNQ
                     TTILKERLANGETTDDILPEAFATCREAGWRVLGMKHFPVQIIGGIVLHQGRIAEMKT
                     GEGKTLVATLPAYLNALTGEGVHIVTVNDYLARRDSEWMGKLYRYLGLSVGLICHDLD
                     NEGRKKAYAADITYGTNNELGFDYLRDNMVVYKENKVQRPHAFAIVDEVDSILIDEAR
                     TPLIISGKGDKSTDLYAKADAFAKTLKVQRFAELDAKEDMEEYYKENDIDYVVDEKQK
                     TATLTQSGVKKAEEFFGIENLTDPDNLTIQHHVNQAIKANGVMKLDVDYVVKDGEVII
                     VDEFTGRLMYGRRFNEGLHQAIEAKEGVKVQSESKTLATITFQNYFRLYKKLSGMTGT
                     AQTESEEFQEIYKLDVVEIPTNKPVLRKDLPDSVYKTENGKFHAVIDAIVEAHEKGQP
                     VLVGTISIEKSELLSKMLKKRGIKHNVLNAKQHEKEAEIVAQAGKLGAVTIATNMAGR
                     GTDIILGGNAEYMAKAAMRKQGFTEELIEEATGYAETDDEEIINARNTFRELNDKYKE
                     EIKGEAEKVREAGGLYIMGTERHESRRIDNQLRGRAGRQGDPGVSRFFLSTEDDLMRL
                     FGGDRMKMMMERMNVAEDMPIENKMLTSIIEGSQEKVELRNFGIRKDVLQYDDVMNKQ
                     REIIYGQRDQVLNGEDLHETILKMVEDTIATSIKQYLPEGPAEHWNFQSLKDYYAGWL
                     IRDDSKYDFSLEDLEDMEPEEIQKMLVDDALEIYKENEELLPEETIREMERVYLLKNV
                     DTHWMDHIDNMDQLKSGIRLRSYGQHDPVVEYRVEGFDMFDEMIESIREDTVKMMLIM
                     PKRVYEIQKRQDAIAAAKRAAQRAAAAAQSASDDEEAVEQSDAVKQALHREQVAQPTE
                     TSADGTDSVNKTIRKGKKIGRNDPCPCGSGKKYKKCCGRDL"
     gene            complement(5032..6429)
                     /locus_tag="PLS64_05675"
     CDS             complement(5032..6429)
                     /locus_tag="PLS64_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRL43353.1"
                     /translation="MKKILRRSTLIYIVALAFIGGLGYLAFELVTGADDWVDQAYNAH
                     IAGDGGLAQAGAIYDRNGTALAQTVDGKRVYNESESVRKSLLHVVGDDSLNISTAVQS
                     MYRSQLIGYNLVFGLNMPESLRASHDIKLTVDSATCAAAYDVLAAYNKKGACVIYNYK
                     TGEVICSISTMAYDPQAPPEITEDNESEYEGVYLDNVLSSTFTPGSIFKIVTSAAAIE
                     NIPDLYDRTWVCNGSENIGGSEVTCTETHGTLTYKEAMAHSCNIVFAKLAVELGSDTM
                     TKTAEKIGINSSFDVDGIKTAKGSYDVSDANENQLGWSGVGQYNDKVNPMQMAIICGA
                     IANGGKATNPTLIKDESILSALGINYKSSGQELFSSDTAAKLDDVMRYTVSDYYGDNL
                     FGGLTVCAKTGTGEVGDDKNPNGWMVGYSKDEDCPLAFACVVQDSGFGFQYAGPVVEA
                     AMIQAAKSLGASAVQ"
     gene            complement(6598..>8066)
                     /locus_tag="PLS64_05680"
     CDS             complement(6598..>8066)
                     /locus_tag="PLS64_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRL43354.1"
                     /translation="DVTVDDPTVSRNHCVLLRRKDGWYVSDTDSKSGTMLNGKRTRGR
                     AKVLIDDTITIGGTSLIVKRGEEFQQPLQSSWFFSKVSDKPAMKSWKLMLLITFFHFF
                     MCVQAMFWNDGTNTMAPLVLFGALAAVEWGFFFISYFVIRRVNFELESLALFLTGIGV
                     MMLIRQSERSAYVQLVAAAIGMIFFCIIIKLIEDPDKVNKLRLPAMICAVGLLGVTIV
                     FGKITNGAANWIYIGSFSFQPSELAKIIFIFIGASSLDVLMTKKNLLEYIIFSAVCVG
                     LLALMGDFGTALIFFVTFLLTAFMRSGDFKTIILAVAAAIFGVTFVLKFKPYVADRFS
                     GWRHVWEHTQDNLGYQQARVLTYMASGGLFGVGIGNGFLKQVAASESDLVFGLVSEEM
                     GVIVAFTLAVAVGAMFIYARAITTRSRSTFYSISACCAAGLFVVQLSLNVFGATDVLP
                     LTGVTFPFVSAGGSSIMSCWGLVAFIKAADERTYSVKR"
BASE COUNT         2146 a         1881 c         1634 g         2405 t
ORIGIN      
        1 ggagttgttt actgcatagt aatagttatc attaaaatcg ttgatgtttg taagtcctgc
       61 tacgtggtcc gtaaattctt tgtagtgttc cccaccctta tacatattac cgaagtagag
      121 aggatacttc cagtccgatt tatgatctaa agcaatgttg cttatgtagc tgttgaagtt
      181 atcaaactga taccagtaat tttcaatatc accatcgttg ttcttaccct gacactgaag
      241 ataccctttc tcctgctcca tatcagacca gtagtcaaag tatgttgcgt caacgcctac
      301 gatgtcagga ttatctgaga atgaattttc aacgacagca ccatagttta cattgtctat
      361 ttccgagaat tttgtccagc cttcgcctct ggctgtctta acatatgtag ccgtgttatc
      421 gttgtagccc gaaccatcat atcccgaatc tgtcggatat gatgttgcag aagatgcacc
      481 gttgaggtta tgttcgttcg gatggaatct taaaagcgca cccgccggaa tttcaccttt
      541 gaacaccaca gaaccgctta ccttggtgtc tttggtgtct tttgtaagct caactgttcc
      601 ttcataggca tcgttgtagt caaaagttgc ataaaccttt gtaagcgact tccagtcatc
      661 gtttgaaaca taaacttttt tatatgttgc ctttgactgt ttgtcgggag cttcaacaga
      721 aaggtattta ctttctttga acgagccgtc agttgtaaga taaaattctg tgtcacccga
      781 agcacccgaa atgtcgatgg tcttgatgaa cgggttagtc cactgacttt ttgaagcatc
      841 ataaagagca ttatcggcac tgtatgagtc gttaataccg aggtcgcttg tttgtgtttc
      901 gcccttgttg ctgaagatta catttttatg ggatgactta acatctacat agtagtaatc
      961 gctgtctgaa gatgtcttag tcattgcaac acccggccac tctgcgttga agtcagtatc
     1021 gtttacccaa gaatatgcat aagcttcatt ataggtatta ttgctgttat aaagatagat
     1081 acggcgaaaa tcttttgcaa cagtcgtttt gggaagagta aaattaaccc cggaagaaag
     1141 ctcaattctt gttgcaccgg atggtgcagt ggcttcaaaa actcccgacg aaggatcttt
     1201 gcttaccttt tctgttttaa gaaccgtgcc gtcatcctga gcaaaacgac atgtaacaga
     1261 agaaaaacca ttccattcct tattcttgtt caggttaatg taaatcttct gatccaaaga
     1321 tgttgccgca aatacattaa gcgacatacc cgacacggca catatcgaaa gcatgagggt
     1381 aagtgcaagc acaagagaaa agcttttctt tgcggttatg caaagctttt tacttaattt
     1441 cataattttt tcttctcctt cattgccgat tactttgttt tggcagtcaa agaacaatta
     1501 aacagaactg cataggacag tccaatatat ctgccaacta attatgaaac gcaatttttt
     1561 gaatttgttc ttctttttta cttgtataca agtatataat attttattgc atatttgtag
     1621 aggcaataca gccaaacttt tggtaatatt tttggctatt ttgcatatct gcaagcacga
     1681 tttaatattt ttgaccataa tgtgatttta ttacttattt atcgccattt tttgtgttta
     1741 agataaatta tacccattac acaaccttaa caggcagtta aaaagtcaat ttcggcacta
     1801 aatttgttga tttttaattc ttttttcaaa aatatcaaaa attttatgca ggtttactcc
     1861 aagctgtgcg tgcggaaata tgtattattt tgcgattgct gcttttaaaa tgccccaaaa
     1921 ccagcatttt gcagaatttt tcagatatat ttttataatc aatatctata tctcatcaaa
     1981 ttaatcaccg aacaccttga aaattcggca taaataaagg gcagagtatt gctctctgcc
     2041 cttcaaaatg taattcactt aaatcgcaat tatacaaacc gctttatgca aacatattaa
     2101 agatctctgc cgcagcactt cttgtacttt ttaccgctgc cgcacggaca gggatcgttt
     2161 ctgccgattt tcttgccctt acggatagtc ttgttgacag aatccgtacc gtctgcagaa
     2221 gtttctgtag gctgagcaac ctgctcacgg tgaagcgcct gctttacggc gtccgactgt
     2281 tcaacagctt cttcatcatc ggatgcagac tgtgccgctg cggcagccct ctgagctgct
     2341 ctctttgccg ctgcaattgc atcctgtctt ttctgaatct cataaacacg ctttggcata
     2401 atgagcatca tcttgacagt atcttcacga atgctttcaa tcatctcatc aaacatatcg
     2461 aagccttcaa ctctgtactc aacaacagga tcgtgctgac cgtatgaacg aagtctgata
     2521 ccgctcttga gctgatccat attgtcgatg tgatccatcc aatgtgtatc aacattcttg
     2581 aggaggtaaa cacgctccat ctcacggatt gtttcctcgg gaagaagttc ttcgttttcc
     2641 ttgtagattt caagagcatc gtcaacgagc atcttctgaa tctcttcggg ttccatatct
     2701 tcaagatcct caagactgaa atcgtactta gaatcatctc tgataagcca acctgcatag
     2761 taatccttaa ggctctggaa gttccaatgc tctgcaggac cttcggggag atactgcttg
     2821 attgaagttg cgattgtatc ctcaaccatt ttgaggattg tttcgtggag atcctcaccg
     2881 ttaagaacct gatcacgctg accgtagatg atttcacgct gtttgttcat aacatcgtcg
     2941 tactggagga catccttacg aatgccgaag ttacgaagct caaccttttc ctgtgagccc
     3001 tcgataatac ttgtgagcat tttattctca ataggcatat cttcggcaac attcatacgc
     3061 tccatcatca ttttcattct gtcaccgccg aacagacgca taaggtcatc ctcggttgag
     3121 aggaagaaac ggctgacacc cggatcgccc tgacggcctg cacgaccacg gagctggtta
     3181 tcaatacggc gtgactcgtg tctttccgta cccataatgt aaagaccgcc ggcctctctt
     3241 accttttcag cctcgccctt aatttcttcc ttatacttgt cattcagttc tctgaatgta
     3301 tttcttgcat tgatgatttc ttcatcatcg gtttccgcat aacctgtagc ttcttcaata
     3361 agctcctctg taaagccctg cttacgcatt gcggcttttg ccatatactc tgcgttaccg
     3421 ccgaggatga tatcggtacc acgacctgcc atgttggttg cgattgttac cgcaccgagt
     3481 ttacctgcct gagcaacaat ttcagcctct ttttcatgct gttttgcatt gaggacattg
     3541 tgcttgatgc ctctcttttt gagcattttt gaaagaagct ccgacttctc aattgaaatt
     3601 gtaccgacaa ggacaggctg tcccttttcg tgagcctcaa caattgcgtc aataacagcg
     3661 tggaacttgc cgttttctgt cttataaacc gaatcgggga ggtcttttct taaaacaggc
     3721 ttgtttgtag gaatttcaac aacatcaagc ttgtagattt cctggaattc ttccgactct
     3781 gtctgagcag tacctgtcat acccgaaagc tttttgtaaa gacggaagta gttctggaat
     3841 gtgattgtag caagagtctt gctttcgctc tgaaccttta cgccctcttt agcctcaata
     3901 gcctggtgaa gaccttcgtt aaaacgacgg ccgtacataa gacgacctgt aaattcgtca
     3961 acgatgataa cttcgccgtc cttgacaaca tagtcaacat caagcttcat aacgccgttg
     4021 gccttgatag cctgatttac atggtgctga attgtaaggt tatcgggatc ggtaaggttt
     4081 tcaataccga aaaattcttc ggctttctta acaccgctct gtgtgagggt tgctgttttc
     4141 tgcttttcat caacaacata atcaatgtcg ttttccttgt aatattcctc catatcctct
     4201 tttgcgtcaa gttctgcaaa acgctgaact ttaagagttt ttgcaaaagc gtcagccttt
     4261 gcataaaggt ctgttgactt gtcgccctta cccgaaataa taagaggagt tctcgcctca
     4321 tcgataagga ttgagtcaac ctcatcgaca attgcaaatg cgtgcgggcg ctgaaccttg
     4381 ttttccttgt aaactaccat gttgtcacgg aggtagtcaa aaccgagttc gttgtttgtt
     4441 ccgtatgtaa tgtctgcggc ataagccttt tttctgccct cgttatcgag atcgtggcag
     4501 ataagaccga ccgaaagacc gaggtatctg taaagcttgc ccatccactc tgagtcacgg
     4561 cgagcaaggt aatcgttgac tgttacaatg tgaacacctt cgcctgtaag agcgttgagg
     4621 tatgcaggga gggtagcaac caaggtttta ccttcaccgg ttttcatctc ggcgattctg
     4681 ccctggtgaa gaacgatacc gccgataatc tgaacgggga agtgcttcat accaagcact
     4741 ctccagcctg cctcacgaca ggttgcaaat gcttcgggga gaatatcgtc tgttgtttcg
     4801 ccgttggcaa gtctttcttt aagaattgtt gtctggttct tgagttcact ttcgctcata
     4861 gccgcatatt tatcttcgag tgcaagcacc ttgtcacaga ttggttgcac acgcttaact
     4921 tcacgcttgc tgtagttgcc gaacatagcc tttaaaaaat tcatcagggt tcttcctttc
     4981 aatgtaccga aaaaatcggt caaactgtat tttcaataat ataattttaa tttactgcac
     5041 cgcagacgct ccaaggcttt ttgcagcctg aatcattgcg gcctcaacca cgggaccggc
     5101 atattggaaa ccgaaacccg aatcctgtac aacacaggca aaggcgagag ggcagtcctc
     5161 atcttttgag tagccgacca tccagccgtt agggttctta tcatcgccga cttcacccgt
     5221 acctgttttg gcacagactg taagtccgcc gaaaaggttg tcgccgtagt aatccgaaac
     5281 ggtgtatctc ataacatcat caagctttgc cgccgtatcg gatgaaaaaa gctcctgtcc
     5341 gctgctcttg tagtttatgc cgagtgcgga gagtatggac tcatctttga taagggtagg
     5401 gtttgtagcc ttaccgccgt ttgcgattgc accgcagata attgccatct gcatcgggtt
     5461 taccttgtca ttatactgac ctacacccga ccagccaagc tggttttcat tggcatcgct
     5521 gacatcatat gaaccctttg cggttttaat tccgtcaaca tcaaaggagc tgttaatgcc
     5581 gattttctcg gcagtttttg tcattgtgtc acttccgagt tcaacggcaa gctttgcaaa
     5641 aacaatattg caggaatgtg ccattgcctc tttatatgtc agcgttccgt gagtttccgt
     5701 acaggtaact tcacttccgc ctatgttctc acttccgtta caaacccaag tgcggtcata
     5761 aaggtcggga atattctcga ttgccgccgc agatgtaacg attttgaaga ttgaacccgg
     5821 agtaaaggtt gacgaaagca cattatcaag gtaaacgccc tcgtattcgc tttcgttgtc
     5881 ctcggtaatt tcgggaggag cctgcggatc gtaagccatg gtgcttatgg aacagataac
     5941 ctcgcctgtt ttgtagttat atattacaca ggcacctttt ttgttataag ctgcaagaac
     6001 atcataagcc gccgcacagg ttgcggaatc gactgtcagc tttatatcgt gagaggcacg
     6061 caatgattcc ggcatattaa gtccgaaaac aaggttatag cctatcagct gagaacgata
     6121 catactttga acagccgttg aaatgttaag gctgtcatca ccgacaacat gaagcagaga
     6181 ttttcttacg ctctcgcttt cgttataaac tcttttaccg tcaaccgtct gtgcaagagc
     6241 cgtgccgttg cggtcataga tcgctcctgc ctgtgcaagg ccgccgtcac ctgctatatg
     6301 cgcattatat gcctgatcaa cccaatcgtc tgcccctgtt acaagctcaa aggcaagata
     6361 gccgagtccg ccgataaacg caagcgcaac tatatatata agtgtacttc tgcgaagtat
     6421 ttttttcaaa ccttacacca ccctgtgtat caagtgaaca atcgaaaaat taaagaaaat
     6481 gcggctttgc ttgcaaagca ctatgaatac ggtcgggaga tagagcattt tattttgctg
     6541 tttttcctct cgacaacaat tttccatttt caatttttca ttttcaattt actaaactta
     6601 tctttttacc gaataagttc tctcgtctgc ggctttgatg aatgcaacaa gtccccaaca
     6661 agacataata cttgaaccgc cggcagacac aaacggaaat gttacgcctg tgagcggaag
     6721 gacatcggtt gcaccgaaaa cattgagaga aagctgaacg acaaaaagtc ctgccgcaca
     6781 gcacgctgaa attgaataaa aggtacttct gcttcgggtt gtaattgccc ttgcataaat
     6841 aaacattgca cctactgcca cggcaagtgt gaacgcaacg ataactccca tttcttccga
     6901 aacaagtccg aaaacaaggt cactttcgga tgcggcaacc tgctttaaaa aaccgttgcc
     6961 gatacctacg ccgaaaagtc cgccgcttgc catatatgtg agaactcttg cctgctgata
     7021 accgaggttg tcctgagtat gttcccacac atgacgccag cccgaaaaac ggtcggcaac
     7081 atacggttta aacttgagga caaaggttac gccgaaaatt gcggcggcaa cagcaagaat
     7141 aatcgtcttg aaatcgcccg agcgcataaa cgctgtgagc aaaaatgtta caaagaagat
     7201 gagcgcagta ccgaagtcac ccataagagc caaaaggccg acgcatactg ccgagaagat
     7261 aatgtactca agcaggttct tctttgtcat caacacatca agcgaacttg cgccgataaa
     7321 gatgaaaata atctttgcaa gctctgacgg ctgaaatgaa aacgagccga tataaatcca
     7381 gttggcggcg ccgtttgtaa tcttaccgaa tacaattgtt acgcccaaaa gaccgaccgc
     7441 acaaatcatt gccggaagtc tgagtttatt taccttatcg ggatcttcaa tcagcttgat
     7501 aattatgcag aagaaaatca tacctattgc ggcggcaaca agctgaacat aagccgagcg
     7561 ttccgactgt ctgataagca tcattacgcc tatgcccgtc aaaaacaggg cgagcgattc
     7621 aagctcgaaa tttactctgc ggattacaaa ataggagata aagaagaatc cccattcaac
     7681 agccgcaaga gcgccgaaca gcacgagcgg agccattgta tttgtgccgt cattccagaa
     7741 cattgcctga acgcacataa agaagtgaaa aaatgtaata agaagcataa gcttccacga
     7801 tttcattgca ggcttatcgc tgacctttga gaaaaaccag ctcgactgca acggctgttg
     7861 gaactcctcg cctcttttta ctataaggct tgtaccgcct attgttattg tatcgtcaat
     7921 tagcaccttt gctctgcctc ttgttctttt gccgttgagc attgtgccgc tctttgagtc
     7981 ggtatcgctt acataccaac cgtctttacg gcgaaggagt acgcaatgat ttcttgaaac
     8041 tgtcgggtca tcaacggtta catcac
//
LOCUS       DAOMVE010000080         8035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2339_length_8035_cov_3.349875, whole genome shotgun sequence.
ACCESSION   DAOMVE010000080 DAOMVE010000000
VERSION     DAOMVE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8035
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2339_length_8035_cov_3.349875"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            143..1414
                     /locus_tag="PLS64_05685"
     CDS             143..1414
                     /locus_tag="PLS64_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HRL43355.1"
                     /translation="MKQETKCLHSGYVPKNGEPRQMPIYQSTTFKYDSSDAMGRLFDL
                     EDSGYFYTRLQNPSNDMTAAKIADLEGGVAAMLTSSGQAANFFALFNICETGSHIVAS
                     SAIYGGTYNLLGVTMKKMGIDVTFVDQNLPEEELAKAFKPNTRAMFGETITNPTVSVL
                     DIEKFARLAHSHGVPLIVDNTFATPVNCQPIKWGADIVTHSTTKYMDGHGVSVGGAIV
                     DSGNFDWLKYADKYPGLTTPDDSYHGIVYVEKFGKLGYITKATSQLMRDLGSIQSPQN
                     AFYVMNGLESLHVRMERHCKNALEIARFLKANDKVAWVDYPDLEDDKYHALAEKYLPN
                     GSCGVLSFAVKGGRDCAVKFMDSLKLCDIETHVADAKTCILHPASHTHRQMTDEQLIE
                     AGVAPDLIRLSVGLENVDDLIADISQALDKI"
     gene            1472..2170
                     /gene="ispD"
                     /locus_tag="PLS64_05690"
     CDS             1472..2170
                     /gene="ispD"
                     /locus_tag="PLS64_05690"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRL43356.1"
                     /translation="MSAFVSAVIVAAGGSVRMGIADSKQFIPLLSRPAIEYTLKAFQK
                     CHLIKEIVIVCREQDNDRINKIIELNGFSKVSKLVKGGDSRADSVRNGIGACSENAKY
                     YAIHDGARPLITVEEIERVVEAAFDTGAATLGTSVKDTIKVVDGFNNIESTPIRSQLR
                     AVQTPQVFERDLYRFALENAGDNIINFTDDCSLIENMGGEVLVVKGNEENIKLTTPID
                     IVIAESILKSRQQG"
     gene            2190..2663
                     /gene="ispF"
                     /locus_tag="PLS64_05695"
     CDS             2190..2663
                     /gene="ispF"
                     /locus_tag="PLS64_05695"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRL43357.1"
                     /translation="MRIGHGYDVHKFAENRKLIIGGTEIPFELGLLGHSDADVLVHAI
                     MDALLGAAALGDIGKLFPDTDEKYKGADSILLLKEVCRVLADYNYRIVNIDSTVIAQQ
                     PKLKPHIDEMRKNIANACGIDVSLVSVKATTEEKLGFTGRLEGISAHAVCLIEKY"
     gene            2745..3893
                     /locus_tag="PLS64_05700"
     CDS             2745..3893
                     /locus_tag="PLS64_05700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRL43358.1"
                     /translation="MLRKAFVSITLCAVFLLSPFVGLKVNALSADSISAPSAVLMESS
                     TGKVLFEKNPHEQRPCASVTKVMTLLLVFEAVDSGKLSLDDEITASEHAAGMGGSDIW
                     LEKGETMSADDMIKATVVVSANDAAVALAEHISGSEDAFVEKMNSRAKELGMNDTVFK
                     NCNGLDEEGHITSAYDVALMSRELTKHEKIFDYTSIWLDNLRGGKTQIVNTNKLLKTY
                     NGITGLKTGTTNDAGCCMSATATRGDVSLISVVLGCKTGKERFADAAALLDYGFANVS
                     VKELTLPEDMPKSIEVNGGMTDSVKLECKVSSKIVVDKGNGAKITTEIDLPDKIDAPV
                     EKNRKIGTLTYLVNSKKVQSFDICAGDSVQKTSFGALLEYVFASLISL"
     gene            4075..5412
                     /locus_tag="PLS64_05705"
     CDS             4075..5412
                     /locus_tag="PLS64_05705"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005353800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HRL43359.1"
                     /translation="MATKLNDKYLSDFIGEHEYDGVAAEVKAAHKVLHDGSGLGNDFL
                     GWLNLPTDYDKEEFARIKKCAEKIKKDTDVFIVIGIGGSYLGARAAIEFLSSQNYNLT
                     CKDTPQIFFTGNSISSSALAEIMELCEGKDVSVNMISKSGTTTEPAIAFRVFREMLEK
                     KYGKEGARERIYCTTDKAKGTLKALADEEGYETFVVPDDVGGRFSVLTAVGLLPIAVS
                     GADIDALMQGAATAQKEFDNDDLKTNDCYKYAAIRNMLYRKGKTMEVMVSYEPAYTMM
                     SEWFKQLFAESEGKDNKGIFPASVIFSTDLHSLGQYIQDGRRTMFETVVLFDKCKKEV
                     TLGTDPTNVDGLNFLSGKTMGFVNQKAFEGTVLAHNDGGVPNIVLNVPEMNESELGYL
                     IYFFEKACAISGYMLGVNPFNQPGVESYKKNMFALLGKPGYEDQKAALEARLG"
     gene            5448..5864
                     /locus_tag="PLS64_05710"
     CDS             5448..5864
                     /locus_tag="PLS64_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43360.1"
                     /translation="MVTLLIGKKGTGKTKKLISLANEAVAASTGNVVVIEKGSKLTYD
                     VTHKARLIDTEQYLISGYDMLFGFISGICAGNYDVTDILVDSTFKICPEALAGLETFT
                     KKLQDLAETASTNIVLLISADESEIPESINAVCRKV"
     gene            5984..6712
                     /locus_tag="PLS64_05715"
     CDS             5984..6712
                     /locus_tag="PLS64_05715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRL43361.1"
                     /translation="MSDYYNNRKKKRSRREKIGFYTACSICLIAVCMAVYSTYNTLSV
                     PSAKTPAATQSNEVKVNENVTGVTQTLPEVKLDVEVPTIAATEEPSTNPTESSKNALE
                     TMLSTDLSLNYPLNSNNVVREYSEKSVYFKTLNVWKPHTGVDFGGKLGDDVTAMTGGV
                     VTNVCEDKMLGKIVEISTDNVICRYCGLGSVDVNKGDSIDVGKKVGTIGAVPFEAGDE
                     NHIHIEVKIDGKYADPLSFINNNE"
     gene            complement(7005..7637)
                     /locus_tag="PLS64_05720"
     CDS             complement(7005..7637)
                     /locus_tag="PLS64_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRL43362.1"
                     /translation="MSFDKIIAITRQYGSGGHDIGKALAEKLGISFYDKELISIAAKE
                     SGVSPEVFKHADERATNSLLYSLSVGLYNYGNNFSSVGDLPVNDRLYILQHKIIKQLA
                     EKENFVVVGRCADYVLKDNPKLVKVYIYADLDKRIKRAVDRQDIDPARAKQAVLKADK
                     NRANYYSFYSGKKWGLADNYDLCLNSTNLTVDQAVDIIISYINICDSNKK"
BASE COUNT         2454 a         1573 c         1761 g         2247 t
ORIGIN      
        1 atttttcttt tgcttattgc gcttgcagta ttcggtacag atgcgataat gaactttatt
       61 aaagaatttt tcggataatt cggtatccga ttttgagata aaatgagtta aaaatgaatg
      121 aaattacgga ggatttttta caatgaaaca ggaaacaaag tgtttgcatt ccggctatgt
      181 gcctaagaac ggagaaccaa gacagatgcc catttatcaa agcaccacct ttaagtatga
      241 ttcaagtgac gcaatgggca gactttttga ccttgaggac agcggatatt tttatacccg
      301 tctgcagaac ccgtcaaatg atatgactgc cgcaaaaatt gccgaccttg agggcggtgt
      361 tgccgcaatg ctcacatcaa gcggtcaggc ggctaacttc tttgcacttt tcaacatctg
      421 cgaaacaggc agtcacattg ttgcatcgtc tgcaatctac ggcggtacat ataatctgct
      481 cggtgttaca atgaagaaaa tgggcattga tgttactttt gtcgatcaga atcttcccga
      541 agaagagctt gcaaaggcat tcaaaccaaa cacaagggca atgttcggtg aaacaataac
      601 caacccgacc gtgtctgttc ttgatattga aaagtttgca agacttgctc actcacacgg
      661 tgttccgctt attgttgaca acacttttgc aacccctgtc aactgtcagc cgattaagtg
      721 gggtgctgac attgttactc actccaccac caaatatatg gacggtcacg gtgtttctgt
      781 cggcggtgca attgttgaca gcggcaactt tgactggctg aaatatgccg acaaatatcc
      841 gggacttaca actcccgatg attcatatca cggcattgtc tatgtcgaaa aattcggcaa
      901 gctcggctac attacaaagg ctacttctca gcttatgcgt gacctcggtt caatccagtc
      961 accgcagaat gctttttatg ttatgaacgg tcttgaaagt cttcatgtga gaatggaaag
     1021 acattgcaaa aatgctcttg aaattgcaag attcctcaag gctaatgaca aggttgcgtg
     1081 ggttgattat cccgaccttg aagatgacaa atatcacgca cttgctgaaa agtatcttcc
     1141 gaacggctca tgcggtgtac tttcttttgc ggttaagggc ggaagagatt gtgcggttaa
     1201 atttatggac agcttaaagc tctgcgacat tgaaactcat gttgcagacg caaagacctg
     1261 tattcttcat cccgcaagcc acacccaccg tcagatgacg gatgaacagc ttattgaggc
     1321 aggtgttgca cccgacctta tcagactttc ggtaggtctt gaaaatgttg atgatcttat
     1381 tgccgacatc agtcaggctc ttgataagat ttgattttct ttaaaagaaa ttatatataa
     1441 aattgcaaaa taaatttacg gagggttgaa tatgtcagct tttgtatctg cggttattgt
     1501 tgcggcaggc ggttctgtga gaatgggaat tgccgacagc aagcagttta ttccgctttt
     1561 gtcacgacct gcaattgaat acaccctgaa ggcattccaa aaatgccacc ttatcaaaga
     1621 aattgtaatt gtctgccgtg aacaggataa cgacagaatc aataaaatta tcgagcttaa
     1681 cggtttcagc aaggtttcaa aacttgtcaa gggcggtgac agccgtgccg acagcgtgcg
     1741 taacggaatc ggtgcgtgca gtgaaaacgc caaatactat gcaattcatg acggcgcaag
     1801 accgcttatt accgttgaag aaattgagcg tgttgttgag gcggcttttg atacgggtgc
     1861 ggcaactctc ggaacttccg taaaagatac aatcaaggtt gttgacggtt tcaataatat
     1921 agaaagcact cctatccgtt cacagctccg tgcagttcag actccgcagg tttttgagcg
     1981 tgatctgtac cgctttgctc ttgaaaatgc cggcgataat atcatcaact ttacagacga
     2041 ttgttcgctg attgaaaaca tgggcggcga ggttcttgtt gtaaagggca atgaggaaaa
     2101 tattaaactg acaaccccga ttgacattgt gattgccgag agtattctca agtcaagaca
     2161 acagggttga ttgtgcaaag gagatttata tgagaatcgg tcacggatat gatgtgcata
     2221 aatttgccga gaacagaaag ctgataatcg gcggtacaga gattcccttt gaactcggac
     2281 ttctcggtca ttcggatgcc gatgtgcttg ttcacgcaat tatggacgca cttctcggtg
     2341 cggcggctct cggtgatatc ggaaagctgt ttcccgatac agatgaaaag tataagggcg
     2401 cagacagtat tcttcttttg aaagaggtgt gccgtgtgct tgccgattat aactacagaa
     2461 ttgtcaatat cgactcaacc gttattgctc aacagccaaa gctgaaaccg catattgacg
     2521 aaatgagaaa aaatatcgcc aatgcctgcg gaattgatgt ttcgctcgtc agcgttaagg
     2581 ctactaccga ggaaaagctc ggctttacag gcagacttga gggcatttcc gctcacgcag
     2641 tttgtcttat tgagaagtat taaaaagtta cagcttcaaa aaatgcacat attttattac
     2701 atacattcga caccttgttc atattaatga acgaggtgtt ttttatgtta agaaaggcat
     2761 ttgtttcaat aacattgtgt gcagtgtttt tattgtcgcc gtttgtcgga ttaaaagtga
     2821 atgctctcag tgcggacagc atatccgctc cgtctgccgt gcttatggaa tcgtcaacag
     2881 gcaaagttct ttttgagaaa aatccgcatg agcaaagacc gtgtgcgtcc gtaacaaagg
     2941 taatgacgct tttgctcgtc tttgaggctg ttgacagcgg aaaattaagc cttgacgatg
     3001 aaattactgc atccgaacac gccgcaggaa tgggcggaag tgacatttgg cttgaaaaag
     3061 gcgaaaccat gagtgcggat gatatgataa aggcaacggt tgttgtatct gccaacgatg
     3121 cggcggttgc gcttgcggag catatcagcg gaagcgagga cgcttttgtt gaaaaaatga
     3181 acagccgtgc caaagaactc ggaatgaacg acaccgtttt caaaaactgt aacggacttg
     3241 atgaggaggg gcatataacc tctgcctatg atgttgcgct tatgtcaaga gagcttacaa
     3301 agcatgagaa aatttttgac tacacatcaa tttggctcga taatcttcgt ggcggtaaaa
     3361 ctcagattgt caacacgaac aagctgttga aaacatataa cggaattacg ggattgaaaa
     3421 caggcacaac caacgatgcg ggttgctgta tgtctgcaac cgcaacaagg ggagatgttt
     3481 cgctgataag tgttgttctc ggctgtaaaa caggcaaaga gcgatttgca gatgcggcgg
     3541 cacttcttga ttacggcttt gcaaatgttt ccgtgaaaga attaacactt cccgaagata
     3601 tgccgaaaag cattgaagtg aacggcggta tgaccgacag cgtaaagctt gaatgtaagg
     3661 tttcttcaaa aatagtggtt gacaaaggaa acggtgctaa aattacaacc gaaatcgacc
     3721 tgcccgacaa gattgacgct cccgttgaaa agaataggaa gataggtacg cttacatatc
     3781 ttgtcaattc aaaaaaggtg cagagctttg atatttgtgc aggcgattct gttcaaaaaa
     3841 caagcttcgg tgcattgctt gaatatgtgt tcgcttcact gatttcgctt tgattttgtc
     3901 ggaaaatcag gcttttgtgc attgttatta aaagcagtac aaaaaacaat gcgggtttat
     3961 tttaaaaatt cacaaattga ccttgcaaaa acgctcatat tatagtataa taaaacaaca
     4021 aagatactta ttgcgattct attttcgcta taggaatttt ggaggaattt ataaatggca
     4081 acaaagttaa atgacaagta tttaagtgat tttatcggcg aacacgaata tgacggtgtt
     4141 gctgccgaag ttaaagcagc tcacaaggtg ttgcatgacg gcagcggcct cggcaacgac
     4201 tttttgggtt ggctcaatct tcctacagat tatgacaagg aagagttcgc acgcatcaag
     4261 aagtgtgcag agaaaatcaa gaaagatact gatgtattca ttgtaatcgg tatcggcggt
     4321 tcttacctcg gcgcaagagc ggctattgaa tttctttcat cacagaacta caaccttacc
     4381 tgcaaggaca caccgcagat tttcttcaca ggtaactcaa tcagctcatc tgcacttgca
     4441 gaaatcatgg agctttgcga gggcaaggat gtttccgtaa atatgatttc caagtcaggt
     4501 acaacaactg agcctgccat cgctttccgt gtgttccgtg aaatgctcga aaagaagtac
     4561 ggtaaagagg gcgcaagaga gcgtatttac tgcacaactg acaaggctaa gggtacactc
     4621 aaggctcttg cagacgaaga aggttacgaa acatttgttg ttccggatga tgtaggcggc
     4681 cgtttctcgg ttctcacagc agtaggtctt cttcctatcg cagtttcggg tgctgatatt
     4741 gacgctctta tgcagggtgc cgcaacagct cagaaagaat ttgacaacga tgatttaaag
     4801 acaaacgatt gttacaaata tgctgcaatc agaaatatgc tttaccgcaa gggcaaaact
     4861 atggaagtta tggtttctta tgagccggct tacacaatga tgtcagagtg gttcaagcag
     4921 ctctttgcag agagtgaagg caaggataac aagggtattt tccctgcaag cgttatcttc
     4981 tcaacagacc ttcattcact cggtcagtac attcaggacg gcagacgcac aatgtttgaa
     5041 actgttgttc tctttgataa atgtaaaaaa gaagttactc tcggtacaga cccgacaaat
     5101 gttgacggtc ttaacttcct ttcgggcaag acaatgggat tcgtaaatca gaaggctttc
     5161 gagggtacag ttctcgctca taatgacggc ggtgtaccga acattgttct taatgttccc
     5221 gaaatgaacg aatcagagct tggctatctc atctacttct ttgagaaggc ttgtgctatt
     5281 tcaggttata tgctcggtgt taatccgttt aaccagccgg gtgttgaaag ctacaagaag
     5341 aatatgttcg ctcttctcgg caagccgggc tatgaggatc agaaggctgc tcttgaggca
     5401 agactcggat aatttcttaa taaagtataa caacggagga actaattatg gttactttac
     5461 ttatcggtaa aaaaggaaca ggcaaaacta aaaagctcat ctctcttgct aacgaggctg
     5521 ttgccgcttc aacaggcaat gttgttgtaa ttgaaaaagg ctcaaagctc acatatgatg
     5581 ttacacataa ggcaagactt attgatacag agcagtatct catcagcggt tatgatatgc
     5641 ttttcggttt tatcagcggt atctgcgcag gcaactatga tgttactgac attctcgttg
     5701 actcaacatt caagatttgc ccggaggctc ttgcaggcct tgaaaccttc acaaagaagc
     5761 ttcaggatct tgccgaaact gcatcaacta acattgttct ccttatttct gctgacgaaa
     5821 gcgaaattcc ggagtcaatc aatgctgttt gcagaaaagt ataattatac taattgatac
     5881 ttaaaaacaa acttatccct ctcgtttttc gggagggatt tttgtatata caactgtata
     5941 aatttagcac ctcagtcaat aatagaaacg gaggtgcttt tttatgagcg attattataa
     6001 caacagaaag aaaaaacgca gtcgccgtga aaaaatcgga ttttacacgg cttgctcaat
     6061 ttgtctgatt gccgtttgca tggcggtgta ctcaacctac aatacattat cagtgccaag
     6121 tgcaaaaact cctgccgcaa ctcaaagcaa tgaggttaag gttaatgaaa atgtcacggg
     6181 tgtaacccaa accttgcccg aggttaagct tgatgtcgaa gttcctacca ttgcggcaac
     6241 cgaagaaccg tcaacaaatc cgacagaaag ttcaaaaaac gctcttgaaa caatgctttc
     6301 aacagatttg agcctgaact atccgcttaa ttcaaacaat gttgttcgtg agtacagcga
     6361 aaaaagtgtt tatttcaaaa ctttgaatgt atggaaaccg cacacgggag ttgatttcgg
     6421 cggtaaactc ggcgatgatg taaccgcaat gacgggcgga gtggttacta atgtttgtga
     6481 ggataaaatg ctcggcaaaa tcgttgaaat ttcaaccgac aatgtcatat gcagatattg
     6541 cggacttggc tcggtcgatg taaacaaggg tgattcaatc gatgttggga agaaggtcgg
     6601 tacaatcggc gcagtcccct ttgaggcagg ggacgaaaat catatacaca ttgaagtgaa
     6661 gattgatggc aagtacgccg atccgctttc ttttataaat aacaatgaat aattgcgatt
     6721 ttgacgcata caatattaat gcaagccctc gtttcgtttt aaggggcaac aaaaaaggcg
     6781 gatacttttg attgagtatc cgcctctttt tttgagttta tgtcaatgat aaaaaatcac
     6841 ggtaaataaa ttaaaattct gtccgaaaag cgattatctt tcgctctcgt gccattcttc
     6901 gttaccaaag ataaaatcat caagatccat aatgacaaat ttcatatagt acacctcctt
     6961 aataaaacaa tatagtatat tcaggaaatg ctcgaatatg agtgttattt tttatttgag
     7021 tcacaaatat tgatgtaact tataataatg tcaacggcct gatcaactgt caggtttgtg
     7081 ctgttaaggc acaaatcata gttgtctgca agaccccatt tttttcctga ataaaaacta
     7141 tagtaatttg ctctgttctt gtctgccttc aaaactgcct gtttggctct ggcagggtca
     7201 atgtcctgtc tgtcaacagc tcttttaatt ctcttatcaa gatcagcata aatatatacc
     7261 ttcacaagct tcggattatc tttaagaaca tagtcggcgc atctaccgac aacaacgaaa
     7321 ttttcctttt ctgcaagctg cttgataatc ttgtgctgta aaatataaag acggtcatta
     7381 accggcaggt cgcccactga agaaaagttg ttaccgtaat tgtacagtcc cacagacaat
     7441 gaatacaaaa gactgttggt agctctttcg tcagcatgct tgaatacctc gggagaaaca
     7501 ccgctctctt tagcggcaat agaaatgagc tccttatcat aaaacgatat accaagcttc
     7561 tcggcaagtg ctttaccgat atcgtgtccg cctgaaccat actgtcttgt aatggcgata
     7621 attttatcaa aactcataac taaacctccc ggtatttgca atttgtaaag ttgtacagca
     7681 gcaacattaa gtagctaaat gtcaaaacta tatcgcttta catatactat aataacacat
     7741 ttttgcgaaa agtccagtct gtaaaaacaa attttacgaa atttgtacaa aatgaacaaa
     7801 taagtcttat cccaattata taaatgcaca atataattgg gagtgaaatt acatttaaag
     7861 gataagaaca aatttctttt tacatatata tgtatatgaa gagggattca gcgtgtaaaa
     7921 agcagaataa tagtgaaaag gcattaaaaa taagatagaa taaagaataa tatagaaaat
     7981 ttgccgataa tgtaaaaaag ataaaaaaat tgtcaaaaag gggtttacaa aagag
//
LOCUS       DAOMVE010000081         8031 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2342_length_8031_cov_3.932046, whole genome shotgun sequence.
ACCESSION   DAOMVE010000081 DAOMVE010000000
VERSION     DAOMVE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8031
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2342_length_8031_cov_3.932046"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>209)
                     /locus_tag="PLS64_05725"
     CDS             complement(<1..>209)
                     /locus_tag="PLS64_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43363.1"
                     /translation="GMFPDGVCFDGKEEHVWMSNAQFEEYEIGDCVSFFAEVYRYVKT
                     SNGKRIDFALRNPENIKRIEPYELPS"
     assembly_gap    210..219
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<220..512)
                     /locus_tag="PLS64_05730"
     CDS             complement(<220..512)
                     /locus_tag="PLS64_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43364.1"
                     /translation="MKSNIKKENQTKMQKLFPEYYGQKVILIDHDYVIDFDPEEFHKT
                     VGAEKLTYDEYLDIQMKSSHEVRAYFKICYYNIPLGFKGRIERRTKNDICFKR"
     gene            complement(726..2426)
                     /gene="ptsP"
                     /locus_tag="PLS64_05735"
     CDS             complement(726..2426)
                     /gene="ptsP"
                     /locus_tag="PLS64_05735"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRL43365.1"
                     /translation="MLHGTAASEGIGIGKVMLIEEHSLEYTPRTVTDTEAESQRFKAA
                     VDAFCYNTEKQAENLRSSAGEKEAEILAGHIQIIKDPYLSGEIEKLIADGQCAESALE
                     HMCDMFIAMFSAADDELTKQRAADVRDIKSGVLGILLGVNEIKVSDAPKGTVLVAREL
                     TPSVTAGIVKENIAGIITETGGTTSHSAILARALEIPAVLSVEGVASSLKNGDTVVVD
                     GSEGAVIVNPDDNTVAEYSKKRDAFLAERKELENYRGRETKSASGEAYELFCNIGKPE
                     DAVKAVDADGEGVGLFRTEFLFMDRTSIPTEDEQFEAYKKAALILKGKSLIIRTLDIG
                     GDKDIPYLGLEKEENPFMGFRAIRYCLKNRELFKSQIKAILRASAFGDIKIMFPLITT
                     MDELREGKKLVAECKADLRNMGINFNENIQVGVMVETASAAVIADMLAKEADFFSIGT
                     NDLTGYTMACDRGNNDVSYLYSPLQPSVLRMIKRTIECGVQNGISVGMCGEAAANKLM
                     IPLLISFGLTEFSVSAPSVLNVRKIISTWTKEEADKVTAKVLEMSTQQEIVEYLKSVV
                     "
     gene            complement(2462..2725)
                     /locus_tag="PLS64_05740"
     CDS             complement(2462..2725)
                     /locus_tag="PLS64_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRL43366.1"
                     /translation="MVSKELVLTNAQGFHMRPASVFATAMGKYSCDVTIKFNGNQYNA
                     KSLLNIIAACIKCGSEIEVVCDGADENEALAEAVQLIESGLGE"
     gene            complement(2873..4798)
                     /locus_tag="PLS64_05745"
     CDS             complement(2873..4798)
                     /locus_tag="PLS64_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HRL43367.1"
                     /translation="MRITDLLKKSGIALGVKVADKSEAIDKLVSLHEKCGNLKDVKAY
                     KEGILNREELGTTAVGMEVAIPHAKSEAVKAPALTAITVPDGVDYDSPDGKPCKLLFM
                     IAATTDGDVHLEVLARLMQLLMHEDFTAKLKAAKTPEEFISIIDARETEKFPDEPKAE
                     VPAKKGYRVLAITACPTGIAHTYMAAESLQKAGDSLNIPIKVETNGSGGAKNVLTEEE
                     IANCDGIIIAADITINLARFDGKPVLQCAVSDGIHKPEELINKIVNKEVPVYHHKGGS
                     ADAPRKRGGAYGHLMNGVSHMLPFVVAGGIFIAIAFLIDIACGVNAQEVGSAFGTVTP
                     AAAWFKTLGGVAFNLMVPILSGFIAMSIADRPGLLVGLVGGFLATSGATFMDPAAAET
                     VPSGFLGGLLAGFVGGYLMLGIEKMCDKMPKALEGIKPVLIYPLLGLGGILVVMCAVN
                     PFMGMINSGMSNGLNAIASNPAMMVPLCALLAGMMSIDMGGPFNKAAYAFATLNLANA
                     DDQAYIIMAAVMIGGMVPPIAIALSNTFFKNRWTDEERKNAPVNYVMGLSFITEGAIP
                     YAAGHPLQVIPSCIVGSAVAGALSALFGCKLMAPHGGIFVFATMQGTWYFYLLALAIG
                     AVVSMFMLALLKRPIKK"
     gene            complement(4814..5719)
                     /gene="pfkB"
                     /locus_tag="PLS64_05750"
     CDS             complement(4814..5719)
                     /gene="pfkB"
                     /locus_tag="PLS64_05750"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HRL43368.1"
                     /translation="MIYTVTLNPSIDYIVRLDSLVPGITNRTTAEEYYYGGKGINVSC
                     VLAELDLESTAFGFVAGFTGDAIEKGIRNDRIITDFIKLESGISRINIKIKAGVETEI
                     NCQGPHISEGELERLFSKIDRISDGDTIVIAGNIPNTMPDDVYERILERIKGKDVRIV
                     VDATKKLLLNALKYKPFLIKPNRQELSEMFGVEINTEDDIEKYAKELQKLGAKNVLIS
                     LGGDGAMLIDEFGKRHKAGVLKEKVINTVGSGDSMVAGFVAGYEKTKSYPYALKLGSV
                     CGNATAFLDGLATKEKINELLAKFN"
     gene            complement(5697..6464)
                     /locus_tag="PLS64_05755"
     CDS             complement(5697..6464)
                     /locus_tag="PLS64_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HRL43369.1"
                     /translation="MLTQDRYRAILDLLEEKNSVTVSELTKLLDSSESTIRRDLSALD
                     EMGKLTKVFGGATSITRNEGVFEDAVSNRETVMSDEKDAVAEYCAKLIHDSDFVYIDA
                     GTTTLKLVDHITNTNATYVTNGITHARMLIHKGMQTYIVGGKIKPLTEAVVGAEAIKS
                     INCFNFTKAFMGANGIDIGSGFTTPDIEEGLIKETAINKSYMAFVLADHSKFRRVYSV
                     TFAPLQKCCIITDKLPTPDFSQATVIKEVKNDLHRNA"
     gene            complement(6857..7498)
                     /locus_tag="PLS64_05760"
     CDS             complement(6857..7498)
                     /locus_tag="PLS64_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRL43370.1"
                     /translation="MIEEIGGARLKKSDFIGINECFKPIEREYKRGEIITTYSPENDT
                     ICIIKEGTVYLTTDNAENQRRIISFYEKGDIIVTCIVPESDNRIFYLSAKTNCKIDFV
                     KYSKFTACCEKHCSKHQRIISAYIKKSHKKSLAHTDILCQQTLRSKLLTFFEYIKTEK
                     GKNTFYLPLPLSDLADYLSVDRSAMMREIKKMNEENIIISEKRKITVIEDLSE"
     gene            7769..>8031
                     /locus_tag="PLS64_05765"
     CDS             7769..>8031
                     /locus_tag="PLS64_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRL43371.1"
                     /translation="MRIIKRNGSEEDFNIEKIINAVKKANGSGEHKFLTDEQIEDVAD
                     YVEYKCNKIRRAVSVEEIQDMVEDQLMAKGAFELARRYVRYRY"
BASE COUNT         2125 a         1699 c         1631 g         2566 t
ORIGIN      
        1 gatggcagtt cgtatggctc gattctttta atattctcag gatttctgag tgcaaagtca
       61 attcgcttac cgttgcttgt tttgacataa cggtaaactt ctgcgaagaa tgatacgcaa
      121 tcgccaattt cgtattcttc aaattgggca ttgctcatcc aaacatgttc ttctttaccg
      181 tcaaaacaaa ctccgtcagg gaacattccn nnnnnnnnna tacgcttaaa gcagatatcg
      241 ttctttgttc ttctttcaat acgacctttg aaacctaaag gaatgttgta ataacaaatt
      301 ttaaagtatg ctctaacttc atgagatgat ttcatttgta tatccagata ttcatcgtag
      361 gttaattttt ctgcccctac agttttatga aattcttcag gatcaaaatc tatcacgtaa
      421 tcatgatcta tcagtattac cttttgccca tagtattcgg gaaaaagttt ttgcattttt
      481 gtttgatttt cttttttgat gttgcttttc atttttgtaa cctccgttaa taagtgctgt
      541 taaaaacagc ctgacatgcc aacaaaggtt agaatcttat aggttcatta tagagtattc
      601 aaatgctttt tgcaagagga tagctataat tttatgaaag acaaatcccc gtcagaattt
      661 ctgacgggga ttaatcgtgt ttgcaaatta tttaactgag attaacgcat attaactggg
      721 ttgttttaca caaccgattt gaggtattcg acaatttcct gctgtgtact catttcaagc
      781 acctttgcag taactttgtc tgcctcttct tttgtccatg tggaaattat ttttctcaca
      841 ttgagaactg acggagcgga aacgctgaat tctgtgagtc cgaatgaaat aagcaacgga
      901 atcatcagct tgtttgcggc agcctcaccg cacattccga ctgaaatgcc gttctgaaca
      961 ccgcactcaa ttgtgcgttt aatcattctg agaacgcttg gctgaagggg agagtagagg
     1021 taagacacat cgttgttgcc tctgtcacaa gccattgtgt agcctgtgag gtcatttgtg
     1081 ccgatgctga agaagtctgc ctcttttgca agcatatctg cgataactgc cgcagacgct
     1141 gtttcaacca taacaccaac ctgaatgttt tcgttgaaat ttatgcccat attgcgtaaa
     1201 tcggctttgc attctgcaac aagctttttg ccctcacgaa gttcatccat agttgtgatg
     1261 agtgggaaca taatcttgat gtcaccgaat gcgcttgcac gcaaaatggc tttaatctgt
     1321 gatttgaaaa gttcacggtt tttaaggcaa tatctgattg cacggaatcc catgaacgga
     1381 ttttcttctt tttcaagtcc aaggtacgga atgtccttgt caccgccgat atcaagtgtt
     1441 ctgataatca gcgacttgcc ctttaagata agagccgcct ttttgtaggc ctcaaactgt
     1501 tcgtcctctg tcggaattga agttctgtcc atgaacagaa actctgttct gaaaagtccg
     1561 acaccctcgc cgtctgcatc aactgccttg acagcgtctt cgggttttcc tatgttgcag
     1621 aaaagttcat aagcttctcc gcttgcggat tttgtttctc gtcccctgta attttcaagc
     1681 tctttgcgtt ctgcaagaaa agcgtcacgc tttttggagt attctgcaac tgtgttatca
     1741 tccggattaa caattacagc accctcactg ccgtcaacta caactgtgtc gccgtttttc
     1801 aggcttgatg cgacaccctc aacactgagt acggcgggaa tctcaagcgc tcttgcaaga
     1861 attgcagagt gggaagttgt tccgcctgtt tcagtaataa tgcctgctat gttttctttt
     1921 acaatacctg ccgtaacaga aggagtaagc tcccttgcaa caagcacagt gccttttggc
     1981 gcatcgctta ctttaatttc gtttactccg agaagaatgc cgagaacacc gctcttgata
     2041 tctctgacat cagccgcacg ctgttttgta agctcatcgt cagctgccga gaacattgca
     2101 ataaacatat cgcacatatg ctcaagagca gattccgcac attgaccgtc tgcaatcagc
     2161 ttttcaattt caccgctgag atacggatct ttgataatct gaatgtgacc tgcaagaatt
     2221 tctgcctctt tttcaccggc tgaactgcga agattttctg cctgtttttc agtattgtag
     2281 caaaaagcat ctactgctgc cttaaaacgc tgtgactctg cctcagtatc tgttactgtt
     2341 cgtggagtgt attcaagcga atgttcttca ataagcatta ccttaccgat tccgataccc
     2401 tcggatgcgg ctgttccgtg tagcataatc ttcacctctt gtaactggtc tgtcagggcc
     2461 attattcgcc aagaccgctc tcaatgagct gtactgcctc tgcaagtgcc tcgttttcgt
     2521 cagcaccgtc gcaaacaact tcaatttcac tgccgcactt gatgcaagcc gcaataatgt
     2581 tgaggaggct ctttgcgttg tactgattgc cgttgaattt gattgttaca tcacagctgt
     2641 atttgcccat agctgttgca aaaaccgatg ccggacgcat atggaatccc tgcgcatttg
     2701 tgagtacgag ttcttttgat accataataa aatctccttt ataaaataaa tttgtgcttt
     2761 gaagttttga atctaatgtc gtgaaagttc tttgagtttt gaggggtggc accggctttt
     2821 acaccggtgc cgaggaacaa aacatttcaa aaaacgggta atagcaaatt aattattttt
     2881 taatcggtct tttgagaagt gcaagcataa acattgaaac tactgcaccg attgcaagag
     2941 caaggaggta gaaataccat gtaccctgca ttgttgcgaa tacgaagata ccgccgtgag
     3001 gagccataag cttacagcca aagagagctg aaagtgcgcc tgctactgca gaaccaacga
     3061 tacatgaagg gataacctga agcggatgac ctgctgcgta aggaattgca ccttctgtga
     3121 tgaatgaaag tcccattaca tagtttacgg gagcattctt tctttcttcg tctgtccaac
     3181 gattcttgaa gaatgtgttt gaaagagcga ttgcgattgg aggaaccata ccgccgatca
     3241 taactgctgc cataatgatg tatgcctgat cgtctgcgtt tgcaaggtta agtgttgcga
     3301 atgcataagc tgccttgttg aaaggaccgc ccatgtcgat agacatcata cctgcgagga
     3361 gcgcacagag cggaaccatc attgcagggt tagaggcaat agcgttaagg ccgtttgaca
     3421 tacctgagtt aatcataccc atgaacgggt tgactgcgca cattacaacg agaataccgc
     3481 cgagtccgag aagcggatag atgagaacgg gcttgatacc ctcaagagcc ttaggcatct
     3541 tatcgcacat tttttcaata ccgagcatta agtaaccgcc tacgaaacct gcaagcaagc
     3601 cgccgaggaa gcctgacgga actgtttctg cagcggcagg atccataaag gttgcgcctg
     3661 atgttgcaag gaaaccgcct acaagaccaa cgagaagacc gggacggtct gcaattgaca
     3721 ttgcgataaa gccggaaagg ataggaacca taaggttaaa tgccacaccg ccgagtgtct
     3781 taaaccatgc ggctgctgga gttaccgtac cgaatgcact gccaacctcc tgtgcgttta
     3841 caccgcacgc aatatcaatg aggaatgcga ttgcaatgaa gataccgcct gctacaacga
     3901 acgggagcat atgtgataca ccgttcataa ggtgaccgta agcgccgcct ctcttccttg
     3961 gagcatctgc agaaccgcct ttgtgatgat atacgggaac ttctttgttt acaatcttgt
     4021 tgataagctc ttccggctta tgaataccgt ctgaaacggc acactgtaaa acgggcttgc
     4081 cgtcaaaacg ggcaagattt attgtgatgt cagcggcaat gatgataccg tcacaatttg
     4141 caatttcttc ttcggtaagc acattctttg caccgcccga accgtttgtt tcaaccttaa
     4201 tcggaatgtt aaggctgtcg ccggctttct ggagtgattc agccgccata taagtatgag
     4261 caatacctgt tggacaagct gtgatagcaa gaactctgta acccttcttt gcaggaactt
     4321 ctgcctttgg ttcatcgggg aacttctctg tttctcttgc gtcaataatc gagatgaatt
     4381 cttccggagt ttttgcggct ttaagctttg cggtaaaatc ttcatgcatc aaaagctgca
     4441 taagtcttgc aagtacttca agatgaacat caccgtctgt tgttgcggca atcataaaga
     4501 gcagcttaca gggcttaccg tcgggcgagt cataatctac accgtcgggg actgtgatag
     4561 ctgtgagggc gggagctttt acagcttcgc tcttggcatg gggaattgca acctccatac
     4621 ctacagctgt tgtgccgagt tcttcacggt tgaggattcc ctctttgtag gctttaacat
     4681 ccttgaggtt tccgcacttt tcatgtaatg aaacaagttt gtcaattgct tcgctcttgt
     4741 cggcaacttt aacgccgaga gcaatacccg atttttttaa taaatctgtg atacgcatat
     4801 ttttctctcc tttttaatta aattttgcaa gcaattcatt tattttttct tttgtggcaa
     4861 gtccgtcaag aaatgccgtt gcatttccgc acacactgcc caatttcagc gcataggggt
     4921 agctttttgt tttttcatat cctgccacaa agcctgctac cattgagtcg cccgaaccga
     4981 ctgtgttaat taccttttct ttgaggactc ctgctttgtg tctttttcca aattcgtcaa
     5041 tgagcattgc tccgtcaccg ccgagtgaaa tcagaacatt ttttgcaccg agtttctgca
     5101 actcttttgc atacttttca atatcatcct cggtgttgat ttcaactccg aacatttcgg
     5161 aaagttcctg tctgttcggc ttgatgagga acggcttgta cttgagtgcg ttgagtaaaa
     5221 gtttttttgt tgcgtcaact acaattcgta catccttgcc cttgattctt tcaaggattc
     5281 tttcataaac atcatcaggc atcgtattcg gaatgtttcc tgcaattacg attgtgtcgc
     5341 cgtcggatat tctgtcgatt tttgaaaaca gcctttcaag ttcgccttca cttatgtgag
     5401 gtccctgaca attgatttct gtttcaacgc cggctttgat ttttatgttg attcttgaaa
     5461 ttccactttc aagcttaata aagtcggtga taatcctgtc gttgcgaata cccttttcaa
     5521 tggcgtcacc cgtaaagcct gcaacaaatc cgaatgccgt actttcaagg tcaagctctg
     5581 caagtacgca tgaaacattg attcccttac ctccgtagta atattcttcg gcggttgttc
     5641 ggtttgttat tcccggaaca agcgaatcaa gtctgacaat gtagtcaatt gacggattaa
     5701 gcgttacggt gtaaatcatt ttttacctcc tttatcaccg ttgcctgcga aaagtccggc
     5761 gtaggcagtt tgtcggtgat tatacaacat ttttgcagag gggcaaatgt tacggaatac
     5821 accctgcgaa attttgaatg gtctgcaaga acaaatgcca tataagattt gttgattgcc
     5881 gtttccttta taagaccttc ttcaatgtcg ggggttgtga atcccgaacc gatgtcaatt
     5941 ccgtttgctc ccataaaagc ttttgtaaag ttaaagcagt ttatgctttt tattgcttcg
     6001 gcacccacaa cagcctcggt aagcggtttt atttttccgc ctactatata tgtctgcata
     6061 cccttgtgga taagcattct ggcgtgtgtt atgccgtttg taacatatgt tgcgtttgta
     6121 tttgtaatgt ggtcaacaag ctttaatgtt gttgttcctg cgtcaatata aacaaagtca
     6181 gagtcatgga taagctttgc acagtattct gcaaccgcat ctttttcatc gctcataacg
     6241 gtttcacgat tgcttaccgc atcttcaaaa acaccctcgt ttctggtgat tgaagttgca
     6301 ccgccgaaaa cctttgtgag ctttcccatc tcatcaagag cggaaagatc tcttcttatt
     6361 gttgactcgg atgagtcaag caactttgtt aattccgaca ccgtgaccga atttttttct
     6421 tcgagcaaat caagtattgc acgatatctg tcctgtgtaa gcattatcct cacctcttga
     6481 ctgtcttgac tatcttgact atattataac acccgttttt taaaagtcaa gcaaaatcgg
     6541 tcaaaaaaat tcaaaaaaat ttaaagacag caaaattagc taacaaaaaa agtgtcgagc
     6601 gttatttaaa aacagtataa ttttaccgtt tttgcaatta aaattggtgt ttttgattat
     6661 ctccattcaa aatcagtcaa attaattatt ttgactataa ttcttttagc gtgttgactg
     6721 caaggtgttt ttatactaca atattaatcg ttgtgtacaa aaaaagcctg caagtttttt
     6781 gcaggctcaa aacggcgaaa tcacgcattt atattttttg ttttttatgt ttttttatac
     6841 atatatatgt tatatgtcat tctgacaaat cttcaatgac tgtgattttt cgcttttcgg
     6901 agattataat attttcctca ttcatctttt ttatttcacg catcattgca cttcggtcaa
     6961 cagaaaggta atctgcaagg tcggaaagag ggagaggaag ataaaaggtg ttttttcctt
     7021 tttcggtttt aatgtattca aaaaaagtca aaagcttgct cctcagcgtc tgctgacaga
     7081 gaatgtctgt gtgagcaaga ctttttttat gagatttttt gatgtatgca cttataatcc
     7141 gctggtgttt actgcaatgc ttttcacaac aggctgtaaa ttttgagtat ttcacgaaat
     7201 caattttgca gtttgttttg gcactcagat aaaaaattct gttgtctgat tcgggtacaa
     7261 tgcaggtgac gattatgtcg cccttttcat aaaaagaaat aattctccgc tgattttcgg
     7321 cattgtcggt tgtcagataa acagttcctt cttttatgat gcaaattgtg tcattttcgg
     7381 gtgaatatgt agttatgatt tcaccccttt tatactctct ttcaataggt ttaaaacact
     7441 cgtttatccc gatgaaatct gattttttaa gccttgcacc gcctatttct tctatcattt
     7501 gcaacactcc ataatgtgtg gtgttttttg tcttgccaca agatatagta tagtatatca
     7561 cataattgtt gcctgtgcaa cagtatttat attgcaatta ttgtataatt ggtcttgcat
     7621 tgcagggata gtttggttcc ttgcaatttt attacataac gcatttttta aacggcatta
     7681 ttgaaaaatt tattttttta ttcggattaa cagatatagg atatttttac caaggaagta
     7741 ccgcaaaata ataaaaaggg gaaggattat gagaattatt aagcgtaacg gctcagaaga
     7801 agatttcaac attgaaaaaa ttattaatgc cgttaaaaaa gcaaacggtt cgggggaaca
     7861 caagttcctt acagacgaac agattgagga tgttgccgac tatgttgaat ataagtgcaa
     7921 caaaatcagg agagcagtgt ccgttgaaga aattcaggat atggttgagg atcagctaat
     7981 ggcaaagggt gcgtttgagc ttgcaagaag atatgtgcgt taccgctaca a
//
LOCUS       DAOMVE010000082         7947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2371_length_7947_cov_3.484415, whole genome shotgun sequence.
ACCESSION   DAOMVE010000082 DAOMVE010000000
VERSION     DAOMVE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2371_length_7947_cov_3.484415"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..875
                     /locus_tag="PLS64_05770"
     CDS             300..875
                     /locus_tag="PLS64_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL43372.1"
                     /translation="MDIRVMKSKKSIFDAFVNLRSKKELRKITVKELCEKALINKSTF
                     YTYYEDMFDLSDKIESEVVDGIVSTISNPQMMIDEPVKFYRNLLGAMTENKALTNTVF
                     SGSQHPNLIVKLSKSLKNMFFENCPEYINDKKFCTLLDYAIYGGYYAFVENQENTNDY
                     SDELIESISVISEHMTKLIKASIPTLKSNNT"
     gene            1069..2370
                     /locus_tag="PLS64_05775"
     CDS             1069..2370
                     /locus_tag="PLS64_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRL43373.1"
                     /translation="MEINTLDILKNLAIILVAAKLLGIVARKLGAPQVVGEIIAGLIV
                     GPCMLNFVSYDGFISGMAEIGVILLMFSAGLGTDLKELIKTGPVALLVAFAGVIVSVA
                     GGAAVYLVFGFGDGDLAMLEAVFMGTILAATSVSITVQALKEMGHLKGRVATTILSAA
                     IIDDVIGIVLLTVVIGFKSPDVQPASVCINIVLFFVLAFVLGFALYFLFKWLDKLWPH
                     SRRIPIFGLVLCFACAYCAEEFFGIADITGAYVAGIILCNIKDSQYIERKMDVSSYMI
                     FGPVFFASIGLQTSFNDFNLSMLWFSIALVAVALLTKVIGCGGTAKLCKFNMPDSLKI
                     GVGMMNRGEVGLIVAKKGLDTGLMAPQFFAPIIILIIVSSLATPILLKVVYKKWPGKE
                     NEETDNRSEKLAENEKYLTQEVVGATYNITEDNFKDKSKDE"
     gene            complement(2444..3424)
                     /locus_tag="PLS64_05780"
     CDS             complement(2444..3424)
                     /locus_tag="PLS64_05780"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HRL43374.1"
                     /translation="MKILVVFTGGTIGCVRKGEVLSPDNEQKYMLTQMYLDRYGDVDF
                     DTAEPYTVLSENLEGCHMNRLADCLQSYDLNSYDGIVVTHGTDTLQYTSAFLAYIFDG
                     LNVPIVLVSANYPLDDSRSNGFENFVGAIDFIKSGSGNGVFVSYKNADLPVTIHRASR
                     LLAHSAYSDELHSIFDESYGKIQNGIFVKNTRYKALKSEFAFDESVRLSDNSNVLKIS
                     ATVGMQYPEITENVKAVLLEGFHSGTLNTDGKALNDFCQKAKAMNIPVFLTGACKGFY
                     YESKLKFDGLNITVLPPASPIAMYIKLWLLPKSEFDKAFLPCAEDFYDND"
     gene            complement(3469..3933)
                     /locus_tag="PLS64_05785"
     CDS             complement(3469..3933)
                     /locus_tag="PLS64_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HRL43375.1"
                     /translation="MNEKMRRKDRETTEERAYEILKNGEYGILSTIGEDGYPYGVPVN
                     FAVEGNKIYFHCAPNSGLKLKNVEYSNKVSFCTVQNNRIDGAKLTSKYESAVVFGTIA
                     KSVKNKNRGLELIVEKYAPEFIESGKRCIEKSGSMTGVYEITIEKITGKENK"
     gene            complement(3933..5222)
                     /locus_tag="PLS64_05790"
     CDS             complement(3933..5222)
                     /locus_tag="PLS64_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRL43376.1"
                     /translation="MAEIKAFRGMRYNTEKAGEISQLCCPPYDIISEEQRLGYISENE
                     YNIIRLELPKEGENPYQTAREILDMWRNRGVLVSEDKPAIYVYEEEFTAYGERKSIKG
                     IIARVHLEEFEKGIILPHEFTLSKAKEDRLNLMKATNCNFSQIYALYMDSEHTTLATI
                     DNESKDTPKLEFTDGEGVTHRLWIVTDENVIAKLCADFADRKLYIADGHHRYETALNY
                     RNYCRENGLSKVGDPCDYQMIYLVDMEHPGLVVFPTHRLVRDLPDFNVEKVLDGCREY
                     FDVTEMNGTDNMESELAKLYDEGKKAFGFYVGNGKWYRLVLKSLDIMDKLLPELSEPS
                     RQLDVTVLHSLVLERIFGIDKENMANQINLTYTKFFSEAVEGVDNGKFQCSFVLNPTR
                     VTEIRDVAAAGEKMPQKSTYFYPKMITGMVMNDIGVE"
     gene            5472..5548
                     /locus_tag="PLS64_05795"
     tRNA            5472..5548
                     /locus_tag="PLS64_05795"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5506..5508,aa:Ile,seq:cat)
     gene            complement(5783..6724)
                     /locus_tag="PLS64_05800"
     CDS             complement(5783..6724)
                     /locus_tag="PLS64_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HRL43377.1"
                     /translation="MAAKYNEIQELLRSRADLNARLNLMPYDGTPEIKERGNEKYLYV
                     RKRVAGKQTSTYVGAYTEELYNLLLRNAREAREIRKELRSIDKQLANAGYSEDELSSD
                     VINNIAFARANMKMNIYDQAVLEGVATSFPQTEEIIDNGKISGVTATDVQKILNLKHA
                     WEFILDRDVIASRSDYYMLSHIARVVNEGFFAEGGRIRGVPVTIGGSSYVPPLPNELD
                     VKEKIREIIEESDEVINTAIKLCLYCMKTQIFLDGNKRASVIFANHYLISHGGGFLVI
                     PEKEVPEFKRLLVKYYEGEDITVIADFMKKYCWKKIE"
     gene            complement(6944..7225)
                     /locus_tag="PLS64_05805"
     CDS             complement(6944..7225)
                     /locus_tag="PLS64_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL43378.1"
                     /translation="MSQAQLAELLGVSHYTISSYECNRSDPDDNSKIIIAKLFDVSVD
                     YLIGLIDEPLSFNRNIRSFSLPQSFTKEECGDVLDYIKYLEFKKSKINE"
     gene            7213..7389
                     /locus_tag="PLS64_05810"
     CDS             7213..7389
                     /locus_tag="PLS64_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43379.1"
                     /translation="MPATHRGLCEVPEESDRILEDDIITIWGECDGLYSYESVLGSKV
                     SLPKIDVKYYSIDN"
     assembly_gap    7436..7445
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7585..>7947
                     /locus_tag="PLS64_05815"
     CDS             7585..>7947
                     /locus_tag="PLS64_05815"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRL43380.1"
                     /translation="MYCQKCGKQIADGSVFCQHCGAQIGNVVSDNFSNQNANQNLQHT
                     EKHEEPIKNKEEFIADFRAKHPERMVYIVIFIILEIVSTIVFIMNVSSYIDHHRDENL
                     TGMMIAAFLEIIAIIFACA"
BASE COUNT         2330 a         1634 c         1490 g         2483 t
ORIGIN      
        1 ttctgcgatt gtaggcagtt cttcaataat acaagtatag tataatatat tttcaacaaa
       61 tatacaagtg gtaaaaacga tttttatgta aataattttg tcgattcgcc tgaaatattg
      121 tcagttgctg tgaaaatcat tccaaaaaat agtcatggaa tgcgtgtttc atccgtaatt
      181 tttatttttg acacacttat cccatactgt tataatatac aataacgaaa gcataaacaa
      241 gcgacaatta cgctactttc gttggaaaac cgactaattt taaaatcagg tgattatctt
      301 tggacataag agttatgaaa agcaagaaaa gtatttttga cgcatttgta aatctgcgtt
      361 caaaaaaaga actgcgcaaa attaccgtta aggagctttg tgaaaaagca ctcatcaata
      421 aatcgacctt ctacacatat tatgaagata tgttcgacct ttcggataaa attgaaagcg
      481 aggtagttga cggcattgta agtaccataa gcaatccgca gatgatgatt gacgagcccg
      541 taaaatttta tcgcaatctt ctgggtgcaa tgaccgaaaa caaggcactg acaaacacgg
      601 ttttttccgg ttcacaacat cccaatctta tagtaaagct ttcaaaatca ttgaaaaata
      661 tgtttttcga gaactgcccg gaatatataa acgataagaa attctgcaca cttcttgact
      721 acgcaatcta cggcggatac tatgcctttg ttgaaaatca ggagaacaca aacgattatt
      781 cggatgaact tattgaatcg atttcagtga tttccgagca tatgacaaaa ctcataaaag
      841 caagcattcc aacccttaaa agcaataata cataaaaata ctttttgcat atttttcaag
      901 ctgtattatt cattaatttg tgcaaagtgc gatttttgtc gaacttcaaa atatttattt
      961 aaaatcgctg tcgattgtga tagaatatat tcggttttat aagagagaaa ggagagtcgg
     1021 tcagactgcc gtttaggagg cgtttgaagt ctgtccgaaa aagaagttat ggaaattaac
     1081 acacttgata ttctgaagaa tcttgccata atccttgttg cggcaaagct tctcggtatt
     1141 gttgccagaa agctcggcgc accgcaggtt gtgggtgaga ttattgcagg tcttatcgta
     1201 ggaccttgta tgcttaattt tgtttcgtat gacgggttca tctcaggtat ggctgaaatc
     1261 ggcgttattt tgctgatgtt ctcggcaggt ctgggaacgg atcttaaaga gcttatcaaa
     1321 acaggacctg ttgccttgct tgttgcgttt gccggtgtaa tcgtttcggt tgcaggcggt
     1381 gcggctgttt accttgtatt cggctttggt gacggtgacc ttgcgatgct tgaggctgta
     1441 tttatgggaa caatccttgc ggcaacctcg gttagtatta ctgttcaggc tcttaaagaa
     1501 atgggacacc tgaaaggaag ggttgcaaca acaattcttt cagccgcaat tatagacgat
     1561 gttatcggca tcgtacttct caccgttgta atcggtttca aaagccccga tgttcagcct
     1621 gcgtccgttt gcatcaacat tgtacttttc tttgttcttg catttgtact cggctttgca
     1681 ctttattttc tcttcaagtg gctcgacaaa ctctggccgc actcaagaag aattccgatt
     1741 ttcggccttg ttctctgctt tgcgtgtgca tattgtgcgg aagaattttt cggaattgca
     1801 gacatcacag gcgcatatgt tgcaggtatt atcctctgta acatcaagga ttcacagtac
     1861 atcgaaagaa agatggatgt cagctcgtac atgattttcg gacctgtatt ctttgcaagt
     1921 atcggacttc agacaagctt taacgatttc aacctttcaa tgctctggtt ctcaattgca
     1981 ctcgttgcag ttgcactttt gacaaaggtt atcggctgcg gcggtacggc aaagctttgc
     2041 aaattcaata tgcccgactc gctcaaaatc ggcgtaggta tgatgaaccg tggtgaggtc
     2101 ggtcttatcg ttgccaagaa gggacttgac acaggtctta tggcaccgca gttttttgct
     2161 cctattatta ttctcatcat cgtttcatcg cttgcaacac ctattcttct caaggtagtt
     2221 tacaagaaat ggccgggtaa agaaaatgag gagacagaca accgttcgga aaagcttgcc
     2281 gaaaacgaaa agtatcttac tcaggaagtt gtcggtgcaa cctacaacat cacagaagat
     2341 aatttcaaag ataaaagcaa agacgaataa tttaatacaa taacctaaaa ggacaaatga
     2401 cagtcatcaa gtcatttgtc cttattttta tatctgtaaa ttgtcaatcg ttatcgtaaa
     2461 aatcttctgc acagggcaaa aaagccttgt caaattcact tttcggcaaa agccacagct
     2521 tgatgtacat tgcaatcggt gatgcaggag gcagaactgt tatattaagc ccgtcaaatt
     2581 tcagcttgct ttcgtaataa aagcccttgc acgcacccgt caaaaatacg ggaatattca
     2641 ttgcttttgc tttttgacaa aagtcgttca aagccttacc gtctgtattc agcgtacccg
     2701 aatggaatcc ctcaagtaaa acggctttta cattttccgt aatttcagga tactgcatac
     2761 cgaccgttgc ggaaattttc agcacattgc tgttatccga aagtcggacg ctttcatcaa
     2821 aagcaaactc gctttttaat gccttataac gggtattctt cacaaaaatt ccgttctgaa
     2881 ttttaccgta gctctcgtca aaaatgctgt gcagttcgtc gctgtatgcc gaatgtgcaa
     2941 gcagtcttga cgctctgtga attgtcacgg gcaaatctgc gtttttatac gaaacaaaaa
     3001 caccgtttcc gctgcctgat tttataaagt caatcgcacc gacaaaattt tcaaagccgt
     3061 ttgaacggct gtcatcaagc ggataatttg ccgaaacaag cacaatcggc acatttaagc
     3121 cgtcaaaaat ataagcaaga aatgcagatg tatattgcaa agtgtccgtt ccgtgagtta
     3181 caacaatgcc gtcatagctg ttcaaatcat agctttgcag acagtcggca agccggttca
     3241 tgtgacagcc ctcaagattt tcgctgagga cggtataagg ctcagccgtg tcaaaatcaa
     3301 catcgccgta cctgtcaaga tacatctgcg tgagcatata tttctgctca ttgtcgggcg
     3361 aaagcacctc gcctttacgc acacagccga ttgtaccgcc cgtaaaaaca acaagaattt
     3421 tcatatactc acctctttca tattctgccc ttaaaggtta taaataaact atttattttc
     3481 cttgcccgtg attttttcga ttgtgatttc ataaacgcct gtcatactgc ccgatttctc
     3541 tatgcacctt tttccgcttt cgataaattc gggagcatat ttttcaacaa tcagttcaag
     3601 acctctgttc ttgtttttaa ccgattttgc aatcgtaccg aacacaaccg cactttcgta
     3661 ttttgaagtc agttttgcac cgtcaattct gttgttttga accgtgcaaa acgaaacctt
     3721 gttgctgtat tcaacatttt tcagtttcaa acccgaattc ggagcgcaat ggaaataaat
     3781 tttgttgccc tcaacggcaa aatttacagg cacaccgtag ggatagccgt cctcgccgat
     3841 tgttgacaaa atgccgtatt caccgttttt aaggatttca taggctcttt cttcggtagt
     3901 ttccctgtct tttctacgca tcttttcgtt cattattcaa ctccgatatc gttcataacc
     3961 atgcccgtaa tcatcttcgg atagaaatat gttgacttct gcggcatctt ttcgccggcg
     4021 gcggcaacat cacggatttc ggttactctt gtcggattaa gcacaaacga gcattggaac
     4081 ttgccgttgt caacgccctc aacagcctct gagaagaact tggtgtatgt aaggttaatc
     4141 tgatttgcca tattttcctt atcaatgccg aaaattcttt caagcacaag tgaatggaga
     4201 acggtaacat caagctgtct tgacggttcg ctcaattcgg gaagaagctt gtccataatg
     4261 tcaagacttt tcagcacaag cctgtaccat tttccgtttc cgacatagaa tccgaatgcc
     4321 tttttgccct cgtcatagag ctttgcaagc tcgctttcca tattgtccgt accgttcatt
     4381 tcggtaacat caaaatattc cctgcaaccg tcaagaactt tttcaacatt aaagtccggc
     4441 aaatcacgca caaggcggtg tgtcgggaat acaacaagtc ccggatgctc catgtcaaca
     4501 aggtaaatca tctgataatc acaaggatcg ccaacctttg aaagaccgtt ttcacggcag
     4561 tagtttcgat agtttagagc ggtttcatag cgatggtgac cgtctgcaat atagagcttt
     4621 ctgtcggcaa aatctgcaca aagctttgcg attacatttt catccgtaac aatccaaagt
     4681 ctgtgagtaa caccctcacc gtcggtaaat tcaagctttg gtgtgtcctt agattcatta
     4741 tcaattgttg cgagggttgt atgctcgctg tccatataaa gggcataaat ctgactgaaa
     4801 ttgcagtttg ttgccttcat aagattaagt ctgtcctctt tagcctttga aagagtgaac
     4861 tcgtgcggaa gaatgattcc cttttcaaac tcttcaagat gaacacgggc gatgataccc
     4921 ttgatgctct tgcgttcgcc gtaagccgta aattcttcct cataaacata gattgcaggc
     4981 ttgtcctcgc tcacaagaac acccctgttc cgccacatat caaggatttc tcttgctgtc
     5041 tgatacgggt tttcgccctc tttcggaagc tcaagccgaa tgatattata ctcgttttca
     5101 ctgatataac cgagtctttg ttcctcgctt atgatgtcat aaggcggaca gcaaagctga
     5161 gaaatttcac ctgctttttc cgtgttgtaa cgcattcctc tgaacgcttt tatttctgcc
     5221 atattaaaaa cctccaaatt gtttttcggt caagtgtatc ttcaccgtcc gtataaggca
     5281 attataacac aagcagttga tttgaacaag tgaaaagtaa gtttatttaa atttactgtg
     5341 caaaaccaca atgaatatta cggcataaaa atcattgaca atcaaaggaa agtttgatat
     5401 aatatttttt gcgtaagctg attacgatat ttgacattgc ataaactatt tgtcaaatga
     5461 aaacagaata cgcgccaata gctcagttgg ttagagctac cggctcataa ccggtcggtc
     5521 cggggttcga gtccctgttg gcgcaccaaa aaagaaagta ctcaaacggg tactttcttt
     5581 ttattttacc gttatgcgtg aacagggact cgaaggcgag cgttaagaaa acagtccggc
     5641 ggactgtttt tagcgagagc gttgatgaat ttttagcttt gaacaagccg caacaagcga
     5701 ggtagaataa cttagataga taaaacacct gttggcgcac caaaaaagaa agtactcaaa
     5761 cgggtacttt ctttttttgt ttttactcta tctttttcca acaatacttt ttcataaaat
     5821 ctgcaattac ggttatatct tcaccctcat agtattttac aagcagtctt ttaaattcag
     5881 gcacttcctt ttcaggaatc accagaaatc cgccaccgtg tgaaataaga tagtgatttg
     5941 caaaaattac agaggcacgc ttgttaccgt caagaaatat ttgtgttttc atacaataaa
     6001 ggcacaactt aattgccgta ttgataactt catcactttc ttctataatt tcacggattt
     6061 tttcttttac atccaactca ttaggcaacg gtggaacata tgaagaacct ccgatagtaa
     6121 ccggaactcc tctaattctt ccgccttccg caaaaaatcc ttcattaaca actcttgcta
     6181 tatggctgag catatagtaa tcggaacgac ttgctattac atcacgatcc aaaataaatt
     6241 cccatgcgtg ttttaaattc aaaattttct gaacatctgt tgcagtcaca ccggaaattt
     6301 taccgttatc tatgatttct tcagtttgtg ggaatgatgt agcaacaccc tcaagaacag
     6361 cctgatcata tatattcatt ttcatatttg cacgggcaaa cgcaatattg tttataacat
     6421 cagaagaaag ttcatcttct gaatatcccg catttgcaag ttgtttatct atactgcgaa
     6481 gttctttcct gatttcacgg gcttctctgg cattgcggag gagcaaatta tataattctt
     6541 ccgtatatgc acctacataa gttgatgtct gttttcctgc aacacgcttt ctcacataaa
     6601 gatatttttc attacctcgt tccttaattt cgggagttcc gtcatacggc attaaattta
     6661 accttgcatt aaggtcagca cggcttctta gtaactcttg tatttcattg tattttgcag
     6721 ccattataat tctcctttgg ggtacattct cttgtaaaag tataatacaa ttctccccaa
     6781 cagtcaatgc accttaggga acatttttgc attttaaagc ctaattcgtt ccccaataat
     6841 aaatttcaaa catattatat cccactcaag ttcttttatg aaatccatta acaccaacaa
     6901 attccaaaac aacccttatc tcacaagcgg ataagggttg tttttattca ttaattttac
     6961 tctttttgaa ttcgagatac tttatatagt caagcacatc gccacattcc tcttttgtaa
     7021 atgactgcgg aagagaaaaa gagcgaatgt tgcggttaaa cgagagcggt tcgtcaatca
     7081 gaccgatgag atagtcaacc gacacatcaa aaagttttgc aatgattatt tttgaattat
     7141 catcgggatc actgcggtta cattcgtaag atgatattgt gtagtgcgac acacccaaaa
     7201 gctccgcaag ctgtgcctgc gacacaccgt ggtctttgcg aagttccgga ggaatcggac
     7261 agaattcttg aagatgacat catcacaatt tggggtgaat gtgacggctt gtactcttat
     7321 gaaagcgttt taggaagtaa agtatccttg ccgaaaattg atgtaaaata ttattcaata
     7381 gacaattaaa acaacaggct gtacgcaatt ttgtacagcc tgtttctttg tgtagnnnnn
     7441 nnnnnactta attataagta ttttgcgaat ttttgtttaa aattatgaga aattttagat
     7501 ttttaattga caagtgtgaa aatctgcatt ataattctca tataagctgt gaattttcac
     7561 agtaatttta aggaggaatt atttatgtat tgtcaaaaat gcggcaaaca aattgctgac
     7621 ggaagtgtat tttgtcagca ctgcggcgca caaataggaa atgttgtatc agataatttt
     7681 tctaatcaaa atgcaaatca aaatttacag cataccgaaa agcatgaaga accaataaaa
     7741 aacaaagaag aatttattgc cgatttccgc gcaaagcacc ctgaacgaat ggtatatata
     7801 gttatattta ttattctaga aattgtttca acaatcgtat ttataatgaa tgtatcatct
     7861 tatatagatc atcaccgtga tgaaaatctc acaggtatga tgattgcggc atttctcgaa
     7921 ataattgcaa taatttttgc atgtgca
//
LOCUS       DAOMVE010000083         7943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2373_length_7943_cov_3.385396, whole genome shotgun sequence.
ACCESSION   DAOMVE010000083 DAOMVE010000000
VERSION     DAOMVE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2373_length_7943_cov_3.385396"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1350)
                     /locus_tag="PLS64_05820"
     CDS             complement(73..1350)
                     /locus_tag="PLS64_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRL43381.1"
                     /translation="MKIYKTALVCLMTCAVAFFGCSCSLLEDDNNESSVPVTTSEKSS
                     TSVKTTSKPKPTEPCVYNEPKTDYAYSTLTDDYVKKLYNQISDNIDSEYEPSFYAEGN
                     LSEYQMTQAVEAYKNDHPEVFWLKSYYEYENFDYETGIWLTYTMSGDKLVTAKKEFNT
                     AVDTISKSVPYGTECEREEYIHNYIINNCDYDEEAAENDDVQDNENDAYGALVDGKAV
                     CEGYSKAFQILCNKADIDCIQLMGIVDSDNHVWNCVKIGGDWYQIDVTWDDVDDFIYD
                     SHEYFNLTDSLMYEEHTLSPKYSEIDAESFLNLESWCNFYVPKCTAEKYNYHNYCYNY
                     KYPTVSNLDDSDNVSTAIAKAAKNGEEHFVVIVDENVNYDDVYDEVRNGYMYDWLTKA
                     NQINSDSPKLNDTCNMLYDEKSNLITFQLEYIN"
     gene            complement(1494..3590)
                     /locus_tag="PLS64_05825"
     CDS             complement(1494..3590)
                     /locus_tag="PLS64_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRL43382.1"
                     /translation="MTYSDGTNSGFSRRNHYVGQPAVKVGSSNFDYYRKPEKSHGFAK
                     RVAFFAYFPLLILWLETDLKIASGFDVLGGFAFTFLFTIVLSAVLVLLCSFSKRRGVN
                     RGIATVLTAVLTIWYGVQMIYYNVFGTMVVVSSVTNGGAGQAFNSIDMIMTAITSRIV
                     FVVLLFVPLAFFIIFGGKLFDFSKVKLKGKLIVLLIAVFFQIGTVLAVGIDRDSSDTK
                     SNYNLYYGELQQVAVCERYGLYTMQRLDISRLMFGYKANIRTNSKPKNKESTADEITK
                     TEQKAQIMSADFSKLTKSTNNDELKSLYEYFDSEEPSYTNEYTGMFKGYNIIFITAES
                     FSQYAIDKDLTPTLWKMYNEGFQFENYYSPAWGVSTTDGEYANLTGLIPKSGVWSFSK
                     SAENNVSFPFTLGEQSRKLGYKTVNAYHNHTYDYYDRDKYLTNIGYDYSAIGKGLDLG
                     ENVWPNSDLKMMQKTVDDYINSDSFSVYYMTVSGHGGYSYNTMSERNADLVKDLKYSD
                     EVKGYLAANIELDKAMEYLLARLEKAGKLDNTLICLTDDHYPYSLDEFDGVSELAGHK
                     VDTTFEQYKNAWLLWSGSMKKPVKVNTYCSSLDILPTLSNMLGLEYDSRMLAGTDVFG
                     NKEPFVVFADRSWISQNGKYNASTGEYTAFKGAKGKDDIDELNNRCNNLFTVSRMILD
                     NNVYAEAFGDTHVTGK"
     gene            3836..5716
                     /gene="htpG"
                     /locus_tag="PLS64_05830"
     CDS             3836..5716
                     /gene="htpG"
                     /locus_tag="PLS64_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRL43383.1"
                     /translation="MAKKQFKSESKRLLDLMINSIYTHKEIFLREIISNASDAIDKLC
                     FISLTDDKVGLDRGDFKIVLKPDKESRTLTITDNGIGMNKDDLENNLGTIASSGSLKF
                     KQSMEEKQDDIDIIGQFGVGFYSAFMVAKKITVNTKKYGEDTAYCWQSSGADGYTISE
                     IEKENAGTEIILEIKDNTDDENYDEFLEQYRIQGLVKKYSDYIRYPIVMDMTKSRVKE
                     ETKDSDKPEYEDYTETVTLNSMIPLWQRRKKDVTQEEYDKFYSEKFMAMDKPLKTIVT
                     SVEGVVTYKALLFIPSVAPYDYYTKEYKKGLQLYSSGVLIMDNCEELLPEHFRFVKGI
                     VDSADLSLNISREMLQHDRHLITIAQNIEKKIKNELTSMLQNDRENYEKFFNAFGRQL
                     KYGVVSDYGMHKEELQDLIMFYSSSEKKLVTLSEYVDRMKEDQKFIYFATGENAAAID
                     TLPQTELIRSKGYEILYCTEEIDEFTLQSLMTYKEKKFCSAMNDDLGIETEENKEDEE
                     NKDALLTFVKETLGDKVAEVTASKKLVSHPVCLTAKGGISFEMEKYFNSVQPDANMKA
                     QRVLELNLSHPAVKKMEEFIKSDVDRAKKYAEVLYSQALLIAGLPLENPSEYTDLVCS
                     IM"
     gene            5955..7937
                     /locus_tag="PLS64_05835"
     CDS             5955..7937
                     /locus_tag="PLS64_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HRL43384.1"
                     /translation="MKHYDEKQMQENILYLEALSEQYPNIQSAAAEMINLHAILDLPK
                     GTEHFLSDIHGEHEAFRHILNNASGSIREKIDDLFSNTLTSEQRAELATLIYYPKEKL
                     SVYKSEITDIEEWYRLTLIRLLAICRHISSKYTRSKVRKTLPKHYAYIIEELMFGDSG
                     KKDRETYHENILHTIIEAGQADVFILSLCDTIKRLIVDKLHIVGDIFDRGARPDIVLD
                     DLMMHHGVDIQWGNHDILWMGAASGSMACIAAALNNAFSYGNLDTIEVGYGISLRDLS
                     LFAKDVYGGGNVERFMPKGPFADSPYTSNDPLLVADMHKAIAVILFKLEGQLVSRNPN
                     FNMSDRRLLDKIDFENATVTVGEKKYPISDTFFPTVDHDYPYELTKTEKRVMEQLKNA
                     FMASEKLKRHIDFLFAKGGMYKCCNNNLLLHGCIPMNKDGSFMEFDTGNCVLSGKALL
                     DELEKIVRQGHSAPENSPERQKGNDYMWYLWCGKHSPLFGREKMCSFERFFIDDSETH
                     TETRNHYYTLYHDEDACVKILKEFGLSENHGHIINGHVPVIRKKGESPIKANGKLIVI
                     DGGFCRAYQDKTGTAGYTLVYNSYGMRIVTHEPFAGIDNAIKSNKDILSTTKVFDTSE
                     NRIRVAQTDDGQTIRNRIEGLSMLLCAYRNGVLKEQ"
BASE COUNT         2384 a         1701 c         1563 g         2295 t
ORIGIN      
        1 gtatggattt gtcatgttct attttacttt attttcgcct tgcagtcaat gttataatca
       61 atcatgtagc atttaattaa tatattcaag ttgaaatgtt atcaaattag acttttcatc
      121 ataaagcata ttgcaagtat cattcaactt tggtgaatca gagttaattt ggttagcctt
      181 tgtaagccaa tcatacatat atccattccg cacctcatca tacacatcat cgtaatttac
      241 attttcatct actataacta cgaaatgctc ttctccgttc tttgccgctt ttgcaattgc
      301 tgtgctgaca ttatcggaat catcaagatt agatactgta ggatatttat aattataaca
      361 atagttgtga taattgtatt tctcagcagt acatttcgga acatagaagt tacaccaaga
      421 ttccaaattc aaaaaagatt ctgcatcaat ctccgagtat tttggagaca gcgtgtgctc
      481 ctcatacata agactgtcgg taagattaaa gtactcatgt gaatcataaa taaaatcatc
      541 aacatcgtcc caagtaacat caatctgata ccaatctccg ccaatcttca cgcaattcca
      601 tacatgattg tctgagtcaa cgattcccat aagttgtatg caatcaatat cagccttatt
      661 acacaaaatc tgaaatgcct ttgagtaacc ctcacatact gccttgccgt caaccaacgc
      721 accgtaggca tcgttttcat tgtcctgaac atcatcgttt tcagccgctt cctcatcata
      781 atcacaatta ttaattatat aattatgtat atattcttct ctttcacatt cagtgccata
      841 agggacactt ttggaaatag tatcaactgc agtattaaac tctttttttg cagtaacaag
      901 tttatcaccg ctcatagtat atgtaagcca aattcctgtt tcataatcaa aattttcgta
      961 ttcatagtaa cttttaagcc agaacacttc agggtggtca tttttatacg cttccacagc
     1021 ctgtgtcatt tgatattcac taagattccc ttcagcgtaa aatgacggtt catattcgga
     1081 atcaatatta tcacttattt gattgtacag ctttttcaca taatcatcag taagtgtgct
     1141 ataggcataa tctgtttttg gttcattata cacacacggc tctgtcggtt taggtttaga
     1201 tgttgttttt acacttgtac tgcttttttc ggaagtagtt acaggtacac tcgattcgtt
     1261 gttatcatcc tcaagcaatg aacacgaaca gccgaaaaat gcaaccgcac aggtcattaa
     1321 acaaactaag gcagttttat atattttcaa agaatcatct caattttatt ttaaactatt
     1381 tattccgtat acggaatacg gcagcagtca aaactgactg tcgccgtatg gatttgtcat
     1441 gttctatttt actttatttt cgccttgcag tcaatgcaaa tttgagcatt aaactacttt
     1501 cccgtaacat gggtatcacc gaaagcctcg gcataaacat tgttgtcgag aatcattctt
     1561 gaaacggtaa agaggttgtt gcatctgtta ttgagttcgt caatatcatc cttgcccttt
     1621 gcgcccttga aagcggtgta ttcgcctgtt gacgcattgt attttccgtt ctgagaaatc
     1681 cagcttctgt cggcaaacac aacgaacggt tctttgtttc cgaaaacatc cgtacctgcc
     1741 aacattctgc tgtcatattc aagaccgagc atatttgaaa gcgtgggcag aatatcaagg
     1801 cttgaacagt atgtgttaac cttgacgggc tttttcattg aaccgctcca caacagccat
     1861 gcatttttgt actgttcaaa ggttgtgtca accttgtgcc ccgcaagttc agacacaccg
     1921 tcaaattcat caagcgaata cggataatgg tcgtccgtaa ggcaaataag ggtgttgtca
     1981 agcttgcctg ctttttcaag tcttgcaaga agatattcca ttgccttgtc aagctcaatg
     2041 tttgcggcaa gatagccctt gacctcatcg ctgtatttca ggtcttttac aaggtctgcg
     2101 ttgcgttcgc tcatggtgtt gtatgaatat ccgccgtgac cgctgaccgt catataataa
     2161 acgctgaagc tgtcggaatt tatgtaatcg tcaaccgttt tctgcatcat cttgaggtcg
     2221 gaattcggcc acacattttc accgaggtca agaccttttc cgattgcaga gtagtcatag
     2281 ccgatatttg taaggtactt gtccctgtcg tagtaatcat aggtgtgatt gtggtaagcg
     2341 ttaactgttt tgtaaccgag ttttctgctc tgctcaccga gagtgaacgg gaagcttaca
     2401 ttattttcgg ctgactttga aaagctccac acaccggatt ttggaatcaa gcctgtaaga
     2461 tttgcatatt caccgtcagt tgtgcttaca ccccacgcag gcgaatagta attttcaaat
     2521 tgaaatccct cgttatacat tttccagagc gtcggagtca aatccttgtc aattgcatac
     2581 tgtgaaaagc tttcggctgt aatgaaaatt atgttgtagc ccttgaacat acccgtgtat
     2641 tcgttggtat agctcggttc ttcgctgtca aaatattcat aaagcgactt taactcatca
     2701 ttatttgtac tctttgtaag ctttgaaaaa tcagcactca taatctgagc tttctgctct
     2761 gtttttgtaa tttcatcggc agttgattct ttatttttcg gcttgctgtt tgtgcgtata
     2821 ttcgctttgt aaccgaacat aaggcggctg atatcaagcc tttgcattgt ataaagtccg
     2881 tagcgttcac acacggcaac ctgttgcaat tcaccgtaat aaagattgta gttgctcttt
     2941 gtgtcggaag aatcacggtc aatgcctacc gcaaggacag ttccgatctg aaaaaacacc
     3001 gcaatcaaaa gtacaatcag cttacccttt aactttactt ttgaaaagtc aaacagcttt
     3061 ccgccgaaaa ttataaagaa agcaagcgga acaaaaagta aaacaacaaa cacaattctg
     3121 cttgttatcg cagtcatgat catatcaatt gaattgaatg cctgccctgc accgccgttt
     3181 gtaacgctgc ttacaacaac cattgtgccg aaaacattat agtatatcat ctgcacaccg
     3241 taccatatcg tcagcacagc cgtgagtacg gttgcaatcc ctctgtttac ccctctgcgt
     3301 tttgaaaaag aacacaacaa aacaagtacc gccgaaagca cgattgtgaa caggaatgtg
     3361 aaagcaaaac cgccgagcac atcaaacccc gacgcaattt taaggtcggt ttcaagccat
     3421 agaatcaaaa gcggaaagta cgcaaaaaat gcgactctct ttgcaaagcc atgacttttt
     3481 tcaggctttc tgtagtagtc aaaatttgaa cttccgactt ttacggcagg ctgtcccaca
     3541 tagtgatttc ttcttgaaaa tccgctgttt gtaccgtctg aatatgtcat ttaattctcc
     3601 tttctttttc aatgcagtta ttaagtagat tataaaacga aaacgccgcc attgcaatga
     3661 caaatttcac caaaattcgt cctatatgac acattcggac tttttatata ttgaaacagt
     3721 tgaccttttg tagtataatt atagtttaat gagaggcttg tgtgaaaagc tatgcaaatt
     3781 gcggttttga acaaggcttc ttaatttatg tattattatg aaaaggaaga tttttatggc
     3841 aaagaaacag tttaaatcag agtcaaaaag acttcttgac cttatgatta actcaattta
     3901 tactcacaaa gaaatttttc ttcgtgagat tatttcaaat gcaagcgacg ctatcgacaa
     3961 gctttgcttc atttctctta ccgatgacaa ggtcggtctt gacagaggcg atttcaaaat
     4021 cgttttaaag cccgacaaag aaagccgtac tcttacaatc acggataacg gtatcggcat
     4081 gaacaaggat gaccttgaaa acaacctcgg cacaatcgca tcctcaggct cactcaaatt
     4141 caagcagagc atggaagaaa agcaggacga cattgacatt atcggtcagt tcggtgttgg
     4201 tttctactcg gcatttatgg ttgcaaagaa gattaccgta aacacaaaga aatatggcga
     4261 ggatacggca tattgctggc agtcaagcgg tgcagacggc tacacaatca gcgagattga
     4321 aaaggaaaat gccggcacag agattattct tgaaatcaag gataacacag acgatgaaaa
     4381 ctatgacgaa ttccttgagc agtacagaat acagggactt gtcaagaagt attccgacta
     4441 catccgctac ccgattgtta tggatatgac aaagagcaga gttaaggaag aaaccaagga
     4501 cagcgacaag cccgaatatg aggattacac ggaaaccgta acactcaaca gcatgattcc
     4561 tctttggcag agaagaaaga aagatgttac tcaggaagaa tacgacaagt tctacagcga
     4621 aaaatttatg gctatggaca agccacttaa aactatcgta acttctgttg agggtgttgt
     4681 aacctacaag gcattgctct ttattccgtc tgttgctcct tatgactact acacaaagga
     4741 atacaaaaag ggacttcagc tttattcaag cggtgtgctt attatggata actgcgaaga
     4801 gcttttgccc gagcacttcc gttttgtaaa gggtattgtt gacagcgcag acctttcgct
     4861 caacatttca agagaaatgc tccagcatga ccgtcacctc attacaattg ctcagaacat
     4921 tgaaaagaag attaaaaacg agcttacttc aatgcttcag aatgaccgtg agaattacga
     4981 aaaattcttc aacgcattcg gcagacagct aaaatacggt gttgtttcgg actacggtat
     5041 gcacaaggaa gaacttcagg accttattat gttctattct tcaagcgaga aaaagcttgt
     5101 tacgctttcg gaatatgttg acagaatgaa agaagatcag aagttcatct actttgcaac
     5161 aggcgaaaat gccgctgcaa tcgacacact tccgcagaca gagcttatcc gctcaaaggg
     5221 ttacgaaatt ctttactgca cagaggagat tgacgagttt actctacagt cactcatgac
     5281 atataaggaa aagaaattct gctcagcaat gaacgatgac ctcggcattg aaaccgagga
     5341 aaataaagag gatgaagaaa acaaggacgc acttcttaca tttgttaagg aaacactcgg
     5401 tgacaaggtt gcagaggtta ccgcatcaaa gaaacttgtt tcacatcctg tttgccttac
     5461 ggcaaagggc ggcatttcat ttgaaatgga aaagtatttc aactcagttc agcctgacgc
     5521 aaatatgaag gctcagcgtg tgcttgagct taacctttca caccctgccg ttaagaagat
     5581 ggaagaattt atcaagagcg atgttgacag agcaaagaag tatgccgagg ttctctacag
     5641 tcaggcgctt ctaatcgcag gacttccgct tgaaaatccg tccgaataca cagaccttgt
     5701 ctgctcaatt atgtaaattt accgatttcc gcaattcagt aagggcgctc attgggcgcc
     5761 cgcaggagca ttgtacgctc ctttacataa atcacacata cattctcaca tttcatgccc
     5821 gatttccgac caaaacggaa atcgggtgtt ttgtttttgc agaaaattcc gcaaaatctt
     5881 ttgcaataat attgatataa tttttgcgtt gtgatacact gatttcagta aaagtttttt
     5941 cggaggcgga cttgatgaaa cattacgatg aaaagcaaat gcaggaaaat attctctatc
     6001 ttgaggcact ttcggagcag tatccgaaca ttcagtcggc ggcggcagag atgattaatc
     6061 ttcacgcaat ccttgatttg ccgaaaggta cggaacattt tctttccgat attcacggtg
     6121 agcacgaggc attcagacat attctcaaca acgcatcggg ttcaatcaga gaaaagattg
     6181 acgacctttt ttcaaatact ctcacatccg aacagcgtgc cgagcttgca acgctgattt
     6241 attatccaaa ggaaaaactc agtgtttaca aaagcgaaat aaccgacatt gaagaatggt
     6301 acagattaac actcataaga ttgcttgcaa tctgccgaca catttcttcc aaatacacac
     6361 gaagcaaggt tcgtaaaact ctgccaaagc attatgccta catcattgag gagcttatgt
     6421 tcggtgattc aggcaaaaaa gaccgtgaaa cctaccacga aaatattctg cacacaatta
     6481 ttgaggcagg acaagccgat gttttcatac tctcgctttg tgacacgata aaaagactta
     6541 ttgttgacaa gctccacatt gtcggcgaca ttttcgacag aggtgcaaga cctgacattg
     6601 tgcttgacga ccttatgatg caccacggtg ttgacattca gtggggcaat cacgatattc
     6661 tttggatggg tgcggcatcg ggcagtatgg cttgcattgc cgctgcactt aacaacgcat
     6721 tttcatacgg caatcttgac acaatcgagg tcggctacgg aataagtctg cgtgacctct
     6781 cgctttttgc aaaggatgtt tacggcggcg gaaatgttga acgctttatg ccgaaaggtc
     6841 cttttgcgga ttcgccctac acctcaaacg atccgttgct tgttgccgat atgcacaagg
     6901 cgattgccgt tattctcttt aagcttgaag gtcagcttgt cagccgcaat cctaacttta
     6961 atatgagcga caggcgattg cttgacaaga ttgattttga aaatgcgacc gtaactgtcg
     7021 gcgaaaagaa gtatccgatt tccgacacct tcttccccac agttgaccat gactatccat
     7081 atgaactgac aaagacggaa aaacgggtta tggaacagct taaaaatgcc tttatggcaa
     7141 gtgaaaagct gaaaagacac attgattttc tgtttgccaa gggcggaatg tacaagtgtt
     7201 gcaacaacaa tcttttgctc cacggctgta ttccgatgaa taaggacggc agttttatgg
     7261 aatttgacac gggcaactgt gtgctttcgg gaaaagcatt gcttgatgag cttgaaaaga
     7321 ttgttcgtca gggacactct gcccccgaaa attctcccga aaggcaaaag ggcaacgact
     7381 atatgtggta tctctggtgc ggaaaacatt caccgctttt cggcagagaa aagatgtgtt
     7441 cgtttgagcg tttctttatt gacgattccg aaactcacac ggaaacacgc aaccattact
     7501 acacgctcta tcacgatgaa gacgcctgcg tgaagatttt gaaagaattc ggtctttcgg
     7561 aaaatcacgg tcacataatc aacggtcatg ttcccgttat ccgcaaaaag ggcgaaagtc
     7621 cgataaaggc taacggcaag ctgattgtga ttgacggcgg attttgcaga gcatatcagg
     7681 acaaaacagg cacggcgggc tacacgcttg tctataattc ctacggtatg cgaattgtca
     7741 cccacgaacc ctttgcggga attgacaacg caatcaaatc aaacaaggac attctctcaa
     7801 caacaaaagt ttttgacacc tcggaaaacc gaatccgtgt tgcacaaacc gatgacggtc
     7861 agacaatccg taaccgcatt gaaggacttt caatgctcct ctgcgcctac cgcaacggtg
     7921 ttctgaaaga acagtaattt aaa
//
LOCUS       DAOMVE010000084         7877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2398_length_7877_cov_2.899386, whole genome shotgun sequence.
ACCESSION   DAOMVE010000084 DAOMVE010000000
VERSION     DAOMVE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2398_length_7877_cov_2.899386"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLS64_05840"
     CDS             complement(<1..476)
                     /locus_tag="PLS64_05840"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HRL43385.1"
                     /translation="MSKTSFAVLTKARSKFGNRLTEKDYQSLLACQSVGEIMSYLKNN
                     THYSKALTDVSEREIHRGRLEALLRQNLFYEFDSLCRYDSSVSSGLSSYIISTLEVEQ
                     IIRFLILLSSNSTDKFIYQFPGYISKHTEIDVNKLANAKNYEEFLNATQSSQFYDIL"
     gene            complement(515..826)
                     /locus_tag="PLS64_05845"
     CDS             complement(515..826)
                     /locus_tag="PLS64_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43386.1"
                     /translation="MENMIKKIVDADNEAKAMEKDTLEEKEELSKTIEAETKKIYDDF
                     MAKAEEVVKRNDIQETKKAEQQWEEIQGKHNSVHIKLDSDFKLNCDKWVDEIVKRTVA
                     Q"
     gene            complement(847..>1373)
                     /locus_tag="PLS64_05850"
     CDS             complement(847..>1373)
                     /locus_tag="PLS64_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015536724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HRL43387.1"
                     /translation="TPAVGDSVAYILEEMGLEVCGCHGTTAALALLNDAVKKGGVMAS
                     SHVGGLSGAFIPVSEDAGMIHAATEGYLDITKLESMTAVCSVGLDMIVVPGDITPEVI
                     SAIIADEAAIGMVNTKTTAVRLIPAIGKSAGEELNFGGLLGRGPVMPVRKGSPEAFIK
                     RGGRIPAPLQALKN"
     assembly_gap    1374..1383
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1384..2364)
                     /locus_tag="PLS64_05855"
     CDS             complement(<1384..2364)
                     /locus_tag="PLS64_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015518925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HRL43388.1"
                     /translation="MLETRDILETINMIDNENLDVRTITMGISLLDCIDEDIDKACTK
                     VYDKICRNAKDLVKTGEDIQKEFGIPIIHKRISVTPIGMVAAPCQSKNVVKFAHTLDK
                     AAKELGVNFIGGYSALVQKGFASGDYALIESIPQALSETDFVCSSVNIGSTKAGINMD
                     AVKMMGKVVKQAAEVTADRNCIGAAKLVVFCNAPEDNPFMAGAFHGVGEADTVINVGV
                     SGPGVVRAALAKDGAGKDITEIADMVKKTAFKITRMGQLVAREASKRLCVPFGIVDLS
                     LAPTPAVGDSVAYILEETKGNLPTWLAPVQVRVMPVKTDNEELMAYAHKVV"
     gene            complement(2398..2667)
                     /locus_tag="PLS64_05860"
     CDS             complement(2398..2667)
                     /locus_tag="PLS64_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRL43389.1"
                     /translation="MKAVITVIGKDSVGILAKVSEACSKADVNIVEVTQSVLQDMFAM
                     IMLVEIDKSNIGFEQLRTNLKAVGESTNTKVHVMHEDIFNSMHRI"
     gene            2826..3647
                     /locus_tag="PLS64_05865"
     CDS             2826..3647
                     /locus_tag="PLS64_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HRL43390.1"
                     /translation="MKICDIHTHSSNSFDAENTVEEMCNSACNKGLFALAITDHCEAP
                     EILLGSESEYGDFSKLIPKSNYETSIMQDRYKGKIKILRGIELGEPMHNAECTKKALC
                     FGDFDIIIASVHNLRNRPDFYYMDFKKEDAHIILDMYFDELLETASFDSFCTLAHLTY
                     PLRYIKRDTGVIPSLSPYRDKIDLIYEKLIKKDKALEINVSGLFKGLGETLPAFDEIK
                     RYHDLGGKYITLGSDAHNINDVGRDIEKGIELAFKAGFKSYYIYENCCPYEITIE"
     gene            complement(3784..4656)
                     /gene="galU"
                     /locus_tag="PLS64_05870"
     CDS             complement(3784..4656)
                     /gene="galU"
                     /locus_tag="PLS64_05870"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HRL43391.1"
                     /translation="MRITKAVIPAAGLGTRVLPATKNMPKEMYPIVDKPTIQFIVEEA
                     VAAGITDILIITNRGKGLMEDHFDASPELESLLEKSGKTEFLETVRNISNLANISFIR
                     QKEMKGLGHAVLKAKSFVGNEPFAVMYGDGVITGKVPAIKQLIDHYGEYGEGVVGVKK
                     VDAKDIHKYGSLKVEHMHDNIFACTDMKEKPQTPEEVLSLYSILDRCVLPAEIFEILE
                     NTKPGIGGEIQLTDAMREIAITKGMTAVEFEGKRYDMGNKFGILQAQIEIGLEHPETK
                     DLLKDYIKNLAKTL"
     gene            complement(4728..5771)
                     /locus_tag="PLS64_05875"
     CDS             complement(4728..5771)
                     /locus_tag="PLS64_05875"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HRL43392.1"
                     /translation="MEFTEYKCPVCDKQFKKGDDVVVCPECGAPHHRECYEKEGHCHF
                     ADKHGADFSFEKEQLEEAEQQAEQDAKDGVVLCKRCGAENPKEMFYCCSCGAPLYGDD
                     KNNPNFQQNQNNGQPNPNFNQNQGMPPFGVPFGQANPQMAAAFDPMAGMKSDEPLVDD
                     ITAGEAAKFIGKNTPYYLRIFSFINKFKKSRFNFSAFILSGIYFLYRKMYGLGVLFSA
                     LVLGSMVGSAYISSLPAWKSIYTGIVQAQQSGQVLSLSNSFGLSPTEFLLFLSPLLAN
                     AVSLIVMVISGLIANKCYYNHSVKKIKKIKSTTNKELLNEALETKGGVNLPIAISVAF
                     AFLVVNYLPMFLM"
     gene            5968..7086
                     /gene="prfB"
                     /locus_tag="PLS64_05880"
     CDS             5968..7086
                     /gene="prfB"
                     /locus_tag="PLS64_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRL43393.1"
                     /translation="MVQFEELLLKLQALKPEIDDLSDALGMKGMLSEIQQLELKATEP
                     GFWDDVEKSQKVLQRTGSLKGKVEAYEGLVAKYEDTHALIELANEEEDESLLEEAESE
                     VEAVKENLEKQRLQTLLTGEYDKNNAILTFHAGSGGTEAQDWAEMLYRMYTRWAEAHG
                     FKVKEVDYLDGDEAGLKSAVLLVEGENAYGYLKSEAGVHRLVRVSPFDSAGRRHTSFS
                     SLEVMPEIAKDTEVHIPPEDIKMDVYRASGAGGQKVNKTSSAVRLTHIPTGIVVASQV
                     ERSQYQNRDVAMNMLISKLVEIKEREHLEKIEDIKGVQKEITWGSQIRSYVFMPYTMV
                     KDHRTGFETGNIQAVMDGDLDGFINAYLKCASLGTLQK"
     gene            7120..>7877
                     /gene="apgM"
                     /locus_tag="PLS64_05885"
     CDS             7120..>7877
                     /gene="apgM"
                     /locus_tag="PLS64_05885"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRL43394.1"
                     /translation="MKYLVMLCDGMADEPNAQLDNSTPMAKAVKPCMDYLASKGEVGM
                     VKTVAEGLKPGSDVANLSVLGYEPAVYYSGRSPLEAASIGIDLKDTDVTLRCNLVTLS
                     DEENYEDKSMVDYCAGDISTEEARELINYVEEKLGNDIFKFYSGVAYRHCLVWKNGNP
                     HPGELTPPHDISGRVIKEYIPKGDDTAALYDLMKKSYDLLKDHPVNKARVEKGLRPAN
                     SIWLWGEGTKPALDSFFGKFGKKGSMISAVDLLKG"
BASE COUNT         2218 a         1666 c         1619 g         2364 t
ORIGIN      
        1 agaatatcgt agaattggga ggactgtgtg gcgttcagaa actcttcata attttttgcg
       61 ttggcaagct tgttaacatc tatttccgta tgctttgaaa tataccccgg gaattgatag
      121 atgaacttgt cagtcgagtt tgaactgagc aggataagga atcttataat ctgctcaacc
      181 tctaatgtcg agataatgta cgatgacaaa cccgagctta cgctcgaatc gtatctgcac
      241 aaagagtcaa attcatagaa aaggttttgc ctcaaaagag cctcaagccg tcctctgtga
      301 atttcacgct cactgacatc cgtcagagcc ttggagtaat gcgtattatt tttaagatac
      361 gacataatct ctccgacgct ctggcatgca agcaaactct ggtaatcctt ttcggtaagt
      421 ctgttgccga actttgacct tgcttttgtc agaactgcaa acgaagtttt tgacattatc
      481 cgcatacacc tccttgtaaa cagtaaatgg aatgttactg tgcaaccgtt cttttgacga
      541 tttcgtcaac ccacttgtca cagttgagtt taaaatcaga gtcaagcttg atatgcactg
      601 aattgtgctt gccctgaatt tcctcccatt gctgttcggc tttctttgtt tcctgaatgt
      661 cgttgcgttt cacaacctct tcagcttttg ccatgaagtc gtcataaatc ttcttcgttt
      721 cggcttcaat agttttggaa agctcctctt tttcttcaag agtatccttt tccatggctt
      781 tggcttcgtt gtcggcatca acgatttttt taatcatatt ctccataaaa ccacccccgt
      841 gaaaaattag tttttaagcg cctgaagcgg agccggaatt cttccgccac gcttaataaa
      901 tgcctccggt gaacctttcc ttacaggcat aacgggacct ctgccgagca gtccgccgaa
      961 attaagttct tcgcctgcac tttttccgat agccggaata agtcttaccg cagttgtttt
     1021 ggtatttacc ataccaattg cagcctcatc tgcaataatt gcagagataa cctcgggagt
     1081 tatgtcaccg ggtactacaa tcatgtcaag accaaccgaa catacggctg tcattgattc
     1141 aagttttgta atatcaaggt aaccttcagt agcggcatga atcatacctg catcctcgga
     1201 aacggggata aaagcaccgc tcagaccgcc gacatgggaa gaagccataa cgccgccttt
     1261 tttaacagcg tcattaagaa gtgcaagtgc agcggttgta ccgtggcatc cgcacacttc
     1321 aagtcccatc tcctcaagaa tatatgcaac actgtcaccg acagcaggtg tcgnnnnnnn
     1381 nnncacaacc ttgtgtgcgt atgccatcag ttcttcgttg tctgtcttaa ccggcatcac
     1441 acgaacctga accggagcca gccatgtcgg cagatttccc ttggtctcct caagaatata
     1501 tgccacactg tcgccgacag caggtgtcgg ggcaagtgaa aggtcaacaa taccaaaggg
     1561 aacacaaagt cgcttgcttg cttctcttgc aacaagctgt cccatacggg tgattttgaa
     1621 tgcagttttc ttaaccatat ctgcaatttc cgtgatgtct tttccggcac cgtcttttgc
     1681 aagagcggct cttacaacgc cgggacccga tacaccgaca ttgataacag tatcggcttc
     1741 accgacaccg tggaaagcac cggccataaa cgggttgtct tcgggagcgt tacagaatac
     1801 aacgagcttt gcggcaccga tgcagtttct gtcggctgta acttctgcag cctgcttaac
     1861 gaccttaccc atcatcttaa cagcatccat attgatgccg gcttttgtag aaccgatatt
     1921 tacggatgaa caaacgaaat cggtttcgct caaggcctgt ggaatactct caataagagc
     1981 gtagtcaccg cttgcaaaac ccttctgaac aagagcggaa taaccgccga tgaagtttac
     2041 gccgagttcc tttgcggctt tgtcaagagt gtgggcaaac ttgacaacat tcttgctctg
     2101 gcacggagcg gctaccatac cgataggggt aactgaaatt cttttatgta taatcggaat
     2161 accaaattcc ttctggatgt cttcacctgt tttaacaagg tcttttgcat ttcgacagat
     2221 tttgtcataa acctttgtgc aagccttatc aatgtcctcg tcaatgcagt caagcaatga
     2281 aatacccatt gtaattgttc ttacatcaag gttttcattg tctatcatat ttatggtttc
     2341 aagtatatct cttgtttcta acatattatg tatatccttt caattcggta gcactgatca
     2401 aattctgtgc atgctgttaa agatgtcctc gtgcataaca tgaacctttg tatttgttga
     2461 ttctccgact gctttgaggt ttgtacggag ctgctcaaat ccgatgttgg atttgtcaat
     2521 ttcaacaagc ataatcatag caaacatatc ctgaagaaca ctctgtgtta cttccacgat
     2581 attaacatca gcctttgaac aggcctctga aacttttgca aggattccaa cgctgtcttt
     2641 accgattact gtgataactg ccttcatttt ttacctcctg tttatgcaga ttttcgttgg
     2701 aattctgcat aaaactgctt ttctttattt gtgcatatta tataactaat ttttaataaa
     2761 gtaaagtgca atattgaaaa aatttttttc atatgttatc atcttttttg tgaggtgttt
     2821 tactaatgaa aatatgtgat attcataccc attcaagcaa ctcttttgat gctgaaaata
     2881 cagttgaaga aatgtgcaac agtgcctgta ataagggact ttttgcactt gcaatcaccg
     2941 accactgcga agctcccgag attttgctcg gctctgaaag tgagtacgga gatttcagca
     3001 aattaattcc aaaatcgaat tatgaaactt cgattatgca agatagatac aagggtaaaa
     3061 tcaaaatact gagaggcatt gaactcggtg agccgatgca caatgccgaa tgtacaaaaa
     3121 aagcactttg ttttggagat tttgacataa taatcgcatc cgtgcataac ctgagaaaca
     3181 gaccggattt ttattatatg gatttcaaaa aagaagatgc acatataatt ctcgatatgt
     3241 actttgatga acttctcgaa accgcctctt ttgacagctt ttgtactctt gcacacctta
     3301 catatccgtt gagatatatt aagcgtgata cgggcgttat tccgagtctt tcaccgtaca
     3361 gagataagat tgaccttatc tacgaaaaac ttattaaaaa ggataaggct cttgaaatta
     3421 atgtttcggg actgttcaag ggactcggtg aaactctacc tgcatttgat gaaattaaac
     3481 gctatcacga ccttggaggc aagtacataa ccctcggaag tgacgcacat aatataaatg
     3541 atgtcggcag agatattgaa aagggaatag agcttgcttt taaagccgga ttcaaatcat
     3601 actatatata tgaaaactgt tgcccttatg aaataacaat tgaataacaa agaattaaaa
     3661 tagttcttaa aagcaaaaag aggatttcgc actgatatga tgtgaaatcc tccttgtttt
     3721 tttgaatcca atatttaact gtagcacagt atactgcact tttctgaaaa ttgcaatacc
     3781 tttttacaaa gtttttgcaa gattttttat gtaatccttc aataaatctt ttgtttcagg
     3841 gtgttcaagc ccaatttcta tctgagcctg caagattccg aatttattgc ccatatcgta
     3901 tctcttgccc tcaaactcaa cggctgtcat tccttttgtt attgcaatct ctctcattgc
     3961 gtctgtaagc tgaatttctc cgccgattcc gggctttgta ttttcgagaa tttcaaaaat
     4021 ttcagccggg agcacgcatc tgtcaagaat cgaataaagt gaaagaactt cttcgggagt
     4081 ctgcggcttt tccttcatgt ccgtacaggc aaaaatattg tcgtgcatat gttcaacttt
     4141 gagcgaaccg tacttgtgaa tgtctttggc gtcaaccttt ttaacgccta caactccttc
     4201 accgtattca ccgtaatgat cgatgagctg tttgattgca ggaaccttgc ctgtaataac
     4261 gccgtcaccg tacataacgg caaaaggttc attgcctaca aaagattttg ctttgagaac
     4321 ggcatgtcca aggcctttca tttccttctg tctgataaaa gaaatgtttg caaggtttga
     4381 aatatttctg actgtttcaa gaaattctgt tttgccgctc ttttcaagca gactttcaag
     4441 ttcgggagaa gcgtcaaaat gatcctccat aagtcctttg ccacggtttg tgatgatgag
     4501 aatatctgta attcctgccg caacagcctc ctcaactatg aactgaatag ttggcttgtc
     4561 aacgatagga tacatttctt tcggcatatt ttttgttgcg ggaagaactc ttgtaccgag
     4621 tcccgccgcc gggataaccg cctttgttat acgcataata ttccttcttt ctcattaaac
     4681 tttttactta ttatgtttct atacatttat tacggtgttt ctgtcattta cattaagaac
     4741 atcggcagat aattgacaac aaggaaagca aaagctacag aaatagcaat cgggagattt
     4801 actccgcctt ttgtttcgag tgcctcattc aaaagctctt tatttgttgt actttttatc
     4861 tttttaattt tcttaacaga gtgattgtag taacatttat ttgcaattaa accgctgata
     4921 accattacta taagtgaaac cgcatttgca agcagaggag agagaaacag taaaaactca
     4981 gtcggtgaaa gtccgaaaga attactaagc gaaagcacct gtccggattg ctgagcctgt
     5041 actatgcctg tgtagataga tttccatgcc ggaagcgatg aaatataagc agaacctacc
     5101 attgaaccga gtacgagagc tgagaacaat acgcctaagc cgtacatttt tcggtacaga
     5161 aaatatattc ccgataaaat aaatgcggaa aaattgaatc tgctcttttt aaacttattg
     5221 ataaagctga aaattctcag ataataaggg gtgttcttac caatgaattt tgcggcttca
     5281 cctgcagtga tatcgtcaac aagcggttcg tcgctcttca ttcctgccat aggatcaaaa
     5341 gcggcagcca tttgcggatt tgcctgacca aaaggaacac cgaacggcgg cataccctga
     5401 ttctgattga aattcgggtt tggctgaccg ttattctgat tctgctgaaa gttcggattg
     5461 tttttgtcat caccgtaaag cggtgcgccg cagctgcagc aatagaacat ttctttcgga
     5521 ttttccgctc cgcagcgctt gcaaagaact acaccgtctt tagcgtcctg ctctgcctgt
     5581 tgttcggcct cttccagctg ttccttttca aaagaaaaat cagcaccgtg tttgtctgca
     5641 aaatgacaat gaccttcttt ttcataacat tctctgtgat gcggtgctcc gcattcaggg
     5701 cagactacaa catcgtcacc ctttttgaac tgtttgtcac ataccggaca tttgtattct
     5761 gtaaattcca ttataaacct ccaaaggata cacgatacgg tgtacctata cactttaatt
     5821 ataacaaata gtattgacaa atgcaacttg tttttgtgta taagtttact tatgaataaa
     5881 atagctgttt ttctttactt ttgtggatgt ttttgctata atattaaatt gtgaaaggat
     5941 aaactatatt cagaaaggta catgtttatg gtacagtttg aggaattact tttaaagctt
     6001 caggctctca agcctgaaat agacgatttg tccgatgcac tcggtatgaa gggcatgctc
     6061 tcggagattc aacagcttga actcaaggca accgagccgg gattttggga cgatgtggaa
     6121 aaatcacaaa aggttttaca gcgtacgggc tctttaaagg gcaaggttga ggcttacgaa
     6181 ggtcttgttg ccaaatacga ggatactcac gctttgattg aacttgcaaa tgaggaagag
     6241 gacgaatctc ttcttgaaga ggcagaaagc gaagttgagg ctgtcaagga aaatcttgaa
     6301 aaacagcgtt tgcagacttt gcttacaggc gaatatgata aaaataacgc aattcttaca
     6361 tttcacgcag gttcgggcgg aacagaggct caggactggg cagaaatgct ttacagaatg
     6421 tacacccgtt gggcagaggc tcacggcttt aaggttaaag aagttgacta ccttgacggc
     6481 gatgaggcag gacttaaaag tgccgtgctt cttgttgaag gtgaaaatgc ttacggctac
     6541 ttaaagagcg aggcaggcgt tcaccgtctt gttcgtgtat ctccgtttga ctcggcaggc
     6601 agaagacaca caagtttttc atctcttgag gttatgccgg aaattgcaaa agatacagag
     6661 gttcacattc cgcccgaaga tatcaaaatg gatgtttacc gtgccagcgg tgcaggcggt
     6721 cagaaggtca acaagacttc atctgctgtt cgtcttactc atattcctac tggtatcgtt
     6781 gttgccagtc aggttgagcg cagtcagtat cagaaccgtg atgttgctat gaatatgctt
     6841 atttcaaagc tcgttgaaat caaagagcgt gagcatcttg aaaagattga ggacatcaag
     6901 ggtgttcaaa aggaaattac ctggggttca cagatccgaa gctatgtatt tatgccgtat
     6961 acaatggtaa aagaccacag aacaggcttt gaaacaggta acattcaggc tgttatggac
     7021 ggcgatcttg acggctttat caacgcatat ttaaagtgtg caagtcttgg cacacttcag
     7081 aaataaattt catattatac ggaaaggcta tgaattagta tgaagtattt agtaatgctc
     7141 tgtgacggta tggctgatga gccaaacgca cagcttgata attcaactcc tatggcaaag
     7201 gctgttaagc cttgtatgga ttaccttgcc tcaaagggcg aggtcggaat ggttaaaacc
     7261 gttgccgaag gactcaaacc cggcagcgat gttgcaaacc tttcggttct cggttatgag
     7321 cctgctgttt attacagcgg tcgctctccg cttgaggcgg caagtatcgg tattgacctt
     7381 aaagacaccg atgtaacatt aagatgtaac cttgtaactc tgtccgatga agaaaactac
     7441 gaggacaagt ctatggttga ctattgtgca ggcgacattt caacagaaga ggcaagagag
     7501 cttatcaatt atgttgagga aaagctcggc aacgatattt tcaagttcta ttcgggtgtt
     7561 gcataccgtc attgtcttgt atggaaaaac ggcaatcctc atccgggcga actcacacct
     7621 cctcacgata tctcaggtcg tgtaattaaa gaatatattc caaagggtga cgatactgcc
     7681 gctctctatg atttgatgaa gaaaagctat gatttgctca aagatcatcc tgtaaacaag
     7741 gcaagagttg aaaaaggact tcgtcccgct aactcaattt ggctctgggg cgagggtaca
     7801 aaacccgctc ttgacagctt cttcggcaag ttcggcaaaa agggcagtat gatttctgct
     7861 gttgaccttc tcaaagg
//
LOCUS       DAOMVE010000085         7870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2401_length_7870_cov_3.597953, whole genome shotgun sequence.
ACCESSION   DAOMVE010000085 DAOMVE010000000
VERSION     DAOMVE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7870
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_7870_cov_3.597953"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..919)
                     /locus_tag="PLS64_05890"
     CDS             complement(161..919)
                     /locus_tag="PLS64_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRL43395.1"
                     /translation="MKKLISWNVNGLRACVTKGFEDFFKDVDADIFCLQETKLQEGQI
                     DLLLEGYHQYWCYADKKGYSGTAMFTKEEPLNVCYGIGIDEHDHEGRVITAEFDNCFV
                     VTCYTPNSQSELKRLDYRMKWEDDFKAYLKKLEEKKPVIMCGDLNVAHKEIDLKNPKT
                     NRKNAGFTDEERSKMTALLDDGFIDTYRYFYPDTEGVYSWWSYRFKAREKNAGWRIDY
                     FIVSKALESKLDGAKIHTEILGSDHCPVELTIDI"
     gene            complement(984..1142)
                     /locus_tag="PLS64_05895"
     CDS             complement(984..1142)
                     /locus_tag="PLS64_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HRL43396.1"
                     /translation="MKYVCDVCGYVYDPEIGDPDNGVDAGTAWEDVPEDWVCPLCGVG
                     KDEFSAEE"
     gene            complement(1241..1783)
                     /locus_tag="PLS64_05900"
     CDS             complement(1241..1783)
                     /locus_tag="PLS64_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HRL43397.1"
                     /translation="MMKWYCTVCGYVYEGPEAPEACPVCKAPKEKFKQMDEEASFATV
                     HEVGVAQGVSEDILKDLRANFEGECSEVGMYLAMARVADREGYPEVSAAFTKYAFEEA
                     EHAAKFAELLGEVLTDSTKKNLQMRVDAETGACEGKFDLAKRAKAQNLDAIHDTVHEM
                     AKDEARHGAGFRGLLNRYFK"
     gene            complement(1806..2198)
                     /locus_tag="PLS64_05905"
     CDS             complement(1806..2198)
                     /locus_tag="PLS64_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRL43398.1"
                     /translation="MAKRNFSSKRNAIYNAVCGTDTHPSARWVYDKLKPEIPDLSLGT
                     VYRNIALFKDEGKVSVICNVNGEERIDGNTEPHPHFVCNKCNAVIDVEDNQNADDLES
                     QLAEEGFKVENRFVIYYGICPKCSAEVH"
     gene            2357..3220
                     /locus_tag="PLS64_05910"
     CDS             2357..3220
                     /locus_tag="PLS64_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRL43399.1"
                     /translation="MKSNKTVKIIIFTALFLSAVILISVMTGAVKETTADTDSVKLPV
                     IMYHSLLKDEKLQNDYTVSPTLFENDLKYLAENGYTTVVVKDLTDYVYGKKSLPEKCI
                     MLTFDDGYYNNYYYALPLLEKYNCKAVISPIASVSEKFTETKDISVTYGHITFDDMKE
                     MSDSGYVEIQNHSYDMHSLKSRKGVLPKAGESDEAYKSILTEDVVKAQGLLENATGKK
                     PTCFVYPFGAKNDLTEKLIKEMGFSCTLTCTEKPNIITKNPDSLYELGRYRRDRNESM
                     QNLLIRIEMQS"
     gene            complement(3224..4828)
                     /locus_tag="PLS64_05915"
     CDS             complement(3224..4828)
                     /locus_tag="PLS64_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRL43400.1"
                     /translation="MTSAKHKNPPAKPKEKYSVQQALTNYYLLIMFTLFPLFFTDAYF
                     NIRHDKYYFFIILTGILVIAEFLIIMTASVDKPPEDSKLEKPKPKHLYEELSFMDWAF
                     IVFLGINVISTLLSANPLDALLGTQGRNNGLVLMAFYTAAYFMITRCFKYFEYIFVAL
                     AFGSMIVYALAVLNSFYIDPLGMFTLLTDQQTITDFTSTIGNKNLLSSYICIAMPVMI
                     AMSVITEKTLLRAIYLIATGFGFAALMTADSDSGILGMAVFMIIYLVWFSNSLVRLKR
                     FFLSATVMLLFAKLLRLFSLCFDDKSKGFDKFQEIFVFSGIGWILLAACAVITGILYL
                     IDYKKPNITISKAVPIALAVVFGLCAVAMIGIMVYFSCIDTKTDLGSFERIIRFNDIW
                     GTHRGFMWIRSIWIFGDASFIEKLFGVGPDMFYSAFSPYFDDLSKYGDSSTNAAHNEY
                     LNYLITIGITGLLSYLAIVCGTIKNAVKYAKENPMLIACVSAVICYAVQSVVNLYQPI
                     TTPLFFIFIALCEAFVRNAKAEKSAV"
     gene            5135..5785
                     /locus_tag="PLS64_05920"
     CDS             5135..5785
                     /locus_tag="PLS64_05920"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRL43401.1"
                     /translation="MKKLVVFGFDGTLADTAPGILYCFNTTAVAMGYEPVEHSALYGV
                     IGAPLEYGFKTLFNMSDDEIEYAVKNYSKLYSQKGKEMFTVYDGIYDALRELKKSGFK
                     LAIATQKHRMFTTDILETYEASDVFDAVCATDVGTNLTKSHLLLQACEQTGVSVEESI
                     LVGDSTIEAKGAEEIGMDFLAVLYGWGFKTKEETEEYKCVGTVSSPAEISQKVMAL"
     gene            6354..>7870
                     /locus_tag="PLS64_05925"
     CDS             6354..>7870
                     /locus_tag="PLS64_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRL43402.1"
                     /translation="MNISHLVEKIKKGDNKSFEKLYKLTEREVWFTCISFLKNETTAQ
                     DIMQETYITAFLKIQSLEKSSQIRSWLNRIAVNKCKNYLKGKGEIQLDDEIFENQAIV
                     DERISIPEEYISDKAKREIILSIMQEVLSDVQYQTVIMHYFNEMTVDEIAEVFECSRG
                     TVLSRLNYSRAKMKTAIEDYENKSGDKLHGVVVVPFFTTIFKEEAKSLAVPNITIKLP
                     NGQTLATSATKGIATGAKSTVSSIVKATATATVKTKVIAVVCGATILAGISAVGISIL
                     AGCNAEKEPTEPSVISSTVQTSTVQTSTVSSEVSKAIEDNGLKIDKDGNITDKNGKKV
                     EVKDGKVEVKTDDGKTVTVKVDDVKTTVSNKNNSNKGNTEKKEDTKKDNTSKTNTSDN
                     DKKPAKPSNSSSNNQKPAKPSESSKAETTAKKYNTSKTNTSKVDTSKNDKKPASKPSN
                     SSKNDTKPAQKPASKPSESSKTETPTAKPKQKVWVDDYKTVHHDGYWKVVGTHEEP"
BASE COUNT         2558 a         1580 c         1532 g         2200 t
ORIGIN      
        1 gtaaagttga atattactga tagtcagtaa gtaataaggg agttatcgtt agatagctct
       61 ctttttttat tctcaaaata cttgtacaga gtaccaatcg gtactctatt tttttactca
      121 gaaaacatat gaaagcggag tgttcaataa gctctgcttt ttaaatgtca atcgtcagtt
      181 caacgggaca atggtcagaa cctaaaattt cggtatggat ttttgcaccg tcaagcttgc
      241 tttcaagagc ttttgaaacg ataaaatagt caatacgcca gcccgcattt ttttcacgag
      301 ccttgaatct gtatgaccac cacgaataca cgccctctgt atcgggatag aaatagcggt
      361 aggtgtcgat aaagccgtca tcgagcaacg ctgtcatctt tgaacgctct tcatcggtaa
      421 agcctgcatt tttacggttg gttttcggat tcttcaaatc aatttctttg tgtgcaacat
      481 tcaaatcacc gcacataata acaggctttt tttcttcaag ttttttcaga tatgccttga
      541 aatcatcttc ccatttcatt cggtaatcaa gccttttcag ctcactttgc gagtttggag
      601 tgtagcaggt gacaacaaag caattgtcaa attctgccgt gataactctg ccctcatggt
      661 cgtgttcgtc aatgccgatc ccgtaacata cattcaaagg ctcttccttt gtgaacatag
      721 ccgttcccga atagcctttt ttgtcggcat agcaccaata ctgatgatat ccttcaagca
      781 agaggtcaat ctgtccctct tgaagctttg tttcctgaag acagaaaata tctgcgtcaa
      841 catctttaaa aaagtcctca aagccctttg taacacaggc acgaagtccg tttacattcc
      901 acgaaatcaa ttttttcata atttactccc gtacacacaa aagggagcaa gcattgcctg
      961 ctcccaaatt gccaatttgt taattattct tctgcggaaa attcgtcttt gcctacacca
     1021 cagagagggc atacccaatc ctcgggtaca tcttcccatg ctgtgcctgc atcaacgccg
     1081 ttgtcgggat cgccgatttc aggatcgtaa acatatccgc aaacatcgca tacatatttc
     1141 atactatcaa atctcctgtt caaagtctgt tgtgtaatcg gttagttgtt ggtcatacga
     1201 ttatcaagct gacttttttg attttttatt gttttaatga ttacttaaag tatctgttaa
     1261 gaagacctct aaagcctgcg ccgtgtcttg cttcatcctt tgccatctcg tgaaccgtgt
     1321 catgaatagc gtcaaggttc tgagcctttg cacgctttgc aaggtcaaac ttaccttcgc
     1381 aagcacctgt ttctgcatct acacgcatct gaagattctt ctttgtgctg tctgtaagaa
     1441 cttcgcctaa aagctctgca aacttggcag cgtgttcagc ctcttcaaaa gcatactttg
     1501 taaatgcggc agaaacctcg ggatagcctt ctctgtctgc aactcttgcc attgcaagat
     1561 acataccgac ttcgctgcat tctccctcaa agttagctct gagatccttg agaatatctt
     1621 cgcttacgcc ctgagcaacg ccaacctcgt gaactgttgc gaaagacgcc tcctcgtcca
     1681 tctgcttaaa tttttccttc ggtgccttac aaacagggca agcctcggga gcttcgggac
     1741 cttcataaac ataaccgcat actgtgcaat accatttcat aattacttct cctttgtaat
     1801 aaatattagt gtacttctgc cgaacattta ggacatatgc cgtagtagat aacaaatctg
     1861 ttttctacct taaatccctc ttcggcaagc tgtgactcaa ggtcatcagc attttgattg
     1921 tcttcaacat caataactgc attgcatttg ttgcagacaa agtgggggtg tggctctgtg
     1981 ttgccgtcaa tacgctcctc accgtttaca ttgcatataa cgctgacctt tccttcatcc
     2041 ttaaaaagag caatgtttct gtaaaccgtg ccaagactaa ggtcgggaat ttcgggcttt
     2101 aatttatcat aaacccagcg tgcgctcgga tgagtgtccg taccgcacac agcgttgtaa
     2161 attgcattgc gttttgagct gaaatttctt tttgccataa ttcataaatc cttatgcaat
     2221 attagtcagt accgattggc caaaatcagt aatcagtatt aactttggct atattctatc
     2281 acgcagatgt ttgattatat ttacaataaa gaattataga taaaaaatct tgcacaaaga
     2341 attgggagga aatatagtga aatcaaacaa aacagtaaaa attatcattt ttactgctct
     2401 cttcctgtct gcggtaattt taatatcggt aatgacaggt gcagtaaaag aaacaacggc
     2461 tgataccgac agcgtaaaac tgcccgtaat aatgtatcac tcacttttaa aggatgaaaa
     2521 actgcaaaat gactacaccg tttcgccgac tctttttgaa aacgatttaa aatatcttgc
     2581 cgaaaacggc tacacaaccg ttgttgtaaa agaccttacc gattatgttt acggcaaaaa
     2641 atcattgccc gaaaaatgca ttatgctgac ctttgacgac ggctattata ataattatta
     2701 ttatgcactc cctttgctcg aaaaatacaa ctgcaaggcg gtcatatctc cgattgcctc
     2761 agtgtccgaa aaattcaccg aaaccaagga catttccgtg acctacgggc atataacttt
     2821 tgacgatatg aaagaaatgt ccgattcggg ctatgttgaa attcagaatc attcgtacga
     2881 tatgcattct ttgaaatcac gaaagggtgt tttgccaaaa gcgggggaga gtgacgaagc
     2941 atataaatct attcttacgg aagatgttgt caaagctcaa ggcttgcttg aaaatgcaac
     3001 agggaaaaaa ccgacctgct ttgtatatcc gttcggtgca aaaaatgacc tgaccgaaaa
     3061 gctgataaaa gaaatgggct tttcgtgtac actaacctgt acggaaaagc ccaatataat
     3121 tactaaaaat cccgacagcc tctacgagct gggacgatac cgccgtgacc gcaacgagtc
     3181 aatgcagaat cttcttatcc gaattgaaat gcagtcataa tatttatacg gcagattttt
     3241 cagccttggc gtttcttaca aaagcctcgc acaaagctat gaagatgaaa aacagcggag
     3301 ttgtaatcgg ctgataaagg tttacaacag actgaacagc atagcagata acagccgaaa
     3361 cgcacgcaat cagcatcgga ttttcctttg catacttaac tgcgttctta atcgtaccgc
     3421 acacaatggc aagataggag agaagtcccg taattccgat tgtgataagg tagtttagat
     3481 attcgttatg ggcggcattt gttgagctgt caccgtattt tgaaaggtcg tcaaagtacg
     3541 gactgaatgc ggagtagaac atatccggac ctacgccgaa cagcttttca atgaatgaag
     3601 cgtcgccgaa aatccatatc gaccttatcc acataaagcc tctgtgcgta ccccatatat
     3661 cgttaaagcg gataattctt tcaaaactgc cgaggtcggt tttggtgtca atacagctga
     3721 aataaaccat aattcctatc atggcaacag cacaaagtcc gaatacaacg gcaagagcaa
     3781 tcggcacggc ttttgaaatt gtgatgtttg gttttttgta atcaataaga tacaaaatcc
     3841 ctgtaataac ggcacaagct gcaagcaata tccagccgat gcctgagaaa acgaaaattt
     3901 cctgaaactt atcaaagccc ttgcttttgt cgtcaaagca gagcgagaac aatctgagca
     3961 gctttgcaaa caacagcata acagttgccg aaaggaaaaa tcttttcagt ctgacaaggc
     4021 tgtttgaaaa ccatacaagg taaataatca taaatacagc catgccgaga atgccggaat
     4081 cgctgtcggc agtcataaga gccgcaaagc caaatcctgt tgcaataaga taaattgccc
     4141 tgagcaaggt tttctccgtg ataaccgaca ttgcaatcat aacaggcata gcgatgcaga
     4201 tgtagcttga aagcagattt ttgttgccga ttgttgaagt gaagtcggta atagtctgtt
     4261 ggtcggtgag aagagtaaac atcccgagag gatcaatata gaatgagtta agcaccgcaa
     4321 gtgcataaac tatcattgaa ccgaacgcaa gcgcaacgaa tatgtattca aaatatttaa
     4381 agcaccttgt aatcataaaa tatgctgcgg tgtaaaatgc cataagcaca agaccgttgt
     4441 ttcgtccctg agtacccaga agtgcatcca aagggtttgc agacaacagc gttgaaatga
     4501 catttatccc gaggaataca ataaatgccc agtccataaa cgaaagctct tcatacaaat
     4561 gctttggttt aggcttttca agctttgagt cttccggcgg tttatcaacg cttgcggtca
     4621 taataataag aaattccgca ataacgagaa tacccgtcaa tataatgaaa aagtaatact
     4681 tgtcatgcct gatgttaaaa tatgcgtcgg taaaaaacaa cggaaaaagc gtgaacataa
     4741 taagcagata atagtttgtg agcgcctgct gtaccgaata tttttctttg ggtttggcag
     4801 gcggattttt atgctttgca gaagtcataa taaaataacc tttcaatata atgtacttca
     4861 attttataac gcacacacga tgttgtcaag aaataaagta agttgaaagc tctggttttc
     4921 gacttgtttc gattggcata tgcttgcaaa agaacaataa ttgcatattt ttcggcactt
     4981 ttattcccaa attaatactt gtggaaaaat tagtattgta tttatctccg taaaatggta
     5041 taattcccct tgagatgtat ttccatattt gcaggacaaa gacttttgta aaaaattgtt
     5101 tatgaattta tctatttgca gattgggtgg ttttatgaaa aaattagttg tttttggctt
     5161 tgacggcact cttgccgaca cagcccccgg aattttatat tgtttcaata caacagcagt
     5221 tgcaatgggc tatgagcctg ttgaacacag cgcactctac ggagtaatcg gcgcaccgct
     5281 tgaatacgga tttaaaacgc tctttaatat gagtgatgac gaaattgaat atgccgtaaa
     5341 gaattacagt aaactttatt cccagaaagg aaaagagatg ttcaccgtct atgacggaat
     5401 ttatgacgct cttcgtgaac tcaagaaaag cggttttaag cttgcaatag caactcaaaa
     5461 gcacagaatg ttcacaacgg atattcttga aacctacgag gcgtcggatg ttttcgacgc
     5521 agtatgtgca acagatgtcg gtacaaacct cacaaagagt catctgcttt tgcaggcgtg
     5581 tgagcagaca ggagtttccg ttgaagaaag tatccttgtc ggcgacagca caatcgaggc
     5641 aaagggtgcg gaagaaatag gtatggactt cctcgctgtg ctatacggct ggggctttaa
     5701 aaccaaggaa gaaaccgaag agtacaagtg cgtcggcaca gtgtcatctc cggcagaaat
     5761 ctctcaaaaa gtcatggcac tctgattacg gttagtgcgt gtaaaacagt gcataaatag
     5821 gattaaaatt gtaaattttc caaaaacaaa tcgggcagtc ataacgattg cctgattttt
     5881 ttatgcaaat ttaattctgc acaataacaa tgatacaacg atatactttt gcttgtaaac
     5941 gggatgtttg ataaacagaa atatgatagt acggaattga tattgttaaa ttcttttaca
     6001 taaattttaa aagttaattt aaataatatt gaattatcac atattgtatg ctaaaataaa
     6061 aatgccaaac aaaaatttaa ttttaagttc acctaagtgt gtatttttat ttcggtaaaa
     6121 atacaggctt tacttttcac accttataag taaaatattg tgtcgcaaca atcaaagcta
     6181 tgtatttttc gtgcgtagct tcagctacgc actttttgtt ttatgcgaaa aatatttgct
     6241 ttttaaaaaa atcgacaaaa tttgaaaaat tttaaaattt tttcggattt tataaaatca
     6301 aaagccgttt taaattgtct atattatgaa aggaaatgag aggaggttat tttatgaaca
     6361 tttcccactt agtagagaaa attaaaaagg gagataacaa atcatttgaa aagctctaca
     6421 agcttacaga gcgtgaagtg tggtttactt gtatcagctt tttaaagaac gaaacaaccg
     6481 cacaggacat tatgcaggaa acttacataa cggctttttt aaaaatccaa tctttggaaa
     6541 aatcatcaca aatcagaagt tggcttaaca gaattgccgt taataagtgc aaaaattatt
     6601 tgaaaggtaa aggtgaaatc caactggacg atgaaatttt tgaaaatcag gcaatagttg
     6661 acgagcgtat atcaattccc gaagaataca tttcagacaa ggcaaagaga gaaattattc
     6721 tttcgattat gcaggaagta ctgtccgatg ttcagtatca gacagtgatt atgcactatt
     6781 ttaatgaaat gactgttgat gaaatcgcag aagtctttga atgttcaagg ggtactgtgc
     6841 tttcaaggct caattattca agggcaaaga tgaaaactgc aattgaagat tacgaaaaca
     6901 aaagcggtga caagcttcac ggtgttgttg ttgtaccttt ctttacaact attttcaagg
     6961 aagaagcaaa gagccttgca gtaccgaaca ttacaatcaa gctcccgaac ggacagacac
     7021 ttgcaacatc tgcaacaaaa ggcattgcaa caggtgcaaa gtcaacagtt tcatctatcg
     7081 taaaggcaac agcaactgcg acagtaaaaa caaaggtaat tgccgttgtc tgcggtgcta
     7141 caatacttgc aggcatatca gcagtcggca taagcattct tgcaggctgt aacgctgaga
     7201 aagaaccgac agaaccgtca gtaatatctt caaccgtaca gacttcaacc gtacagactt
     7261 caactgtatc atctgaggtc agcaaggcta ttgaggataa cggtctaaaa atcgacaaag
     7321 acggcaacat cacagacaag aatggtaaga aagtcgaagt aaaagacggt aaggtagaag
     7381 taaaaaccga tgacggtaag acagttacag ttaaggttga cgatgtaaaa actacagtat
     7441 ctaacaaaaa caacagcaac aagggtaaca ccgaaaagaa agaagatact aagaaagaca
     7501 acacttcaaa gacaaataca tctgataacg acaagaagcc agctaagcca agcaactctt
     7561 caagtaataa tcagaagcca gcgaagccaa gtgagtcatc aaaggctgaa acgacagcta
     7621 agaaatataa cacttcaaag acaaatacat caaaggttga cacttctaag aatgacaaga
     7681 agccagcatc taagccgagc aactcttcaa agaacgatac aaagccagct cagaagccag
     7741 catctaagcc aagtgaatct tcaaagactg aaacacctac agcaaagcca aagcagaagg
     7801 tatgggtaga tgactacaaa acagttcacc atgatggcta ttggaaggta gtaggtactc
     7861 acgaggagcc
//
LOCUS       DAOMVE010000086         7854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2410_length_7854_cov_4.069881, whole genome shotgun sequence.
ACCESSION   DAOMVE010000086 DAOMVE010000000
VERSION     DAOMVE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7854
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2410_length_7854_cov_4.069881"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..1544)
                     /locus_tag="PLS64_05930"
     CDS             complement(321..1544)
                     /locus_tag="PLS64_05930"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRL43403.1"
                     /translation="MADNKIKKVVLAYSGGLDTSIIIPWLKENYDNCEVIAVSGDVGQ
                     GTELDGLEEKAIKTGASKLYIEDLNKEFVDEYIIPTVKAGAVYEGKYLLGTSFARPII
                     AKRLVEIAKAEGADAICHGCTGKGNDQVRFELAIKAYAPDMKIIAPWRTWEFKSREDE
                     IEYAEKHNIPLKINRETNYSKDKNLWHLSHEGLDLENPANEPMYNKEGFLELGVSPEQ
                     APDKAEYVTLTFEKGVPTKLNGEAIDSVELIKELNKIGGRNGVGITDIVENRLVGMKA
                     RGVYETPGGTILYAAHAKLEEICLDKDTLHYKQNVANAFAELVYDGKWYTPLREALSA
                     FVDSTQEYVTGDVKLKLYKGNIIDAGVTSPYSLYDEEIATFDEDQVYDQNDSAGFINL
                     FGLPIKVRAKKGLIK"
     gene            1998..2360
                     /locus_tag="PLS64_05935"
     CDS             1998..2360
                     /locus_tag="PLS64_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRL43404.1"
                     /translation="MKTLNDYLAMSYRMEIVEDRNEGGFVVSFPELPGCIACGDTIES
                     AVANAENAKKAWFEAAFESGVVIHEPDSLEEYSGQFKLRIPKSLHRSLAEHSKKEGIS
                     MNQYCVYLLAKNDAVYSK"
     assembly_gap    2378..2387
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2417..2926
                     /locus_tag="PLS64_05940"
     CDS             2417..2926
                     /locus_tag="PLS64_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HRL43405.1"
                     /translation="MSAFKEINPKEIVESPFKLIGDDWALVTAGDREKFNTMTISWGG
                     VGIMWGKPVVFTFIRPQRYTFAFMENGDRYTMSFFDEKYRDALKFCGSKSGKDYDKVK
                     ETGLTPAFTENGSVYFEEAKLVLECKKMYAQSLNAESITDRESVDKWYDNDFHKMYIS
                     EITKVLVKV"
     gene            complement(3089..7156)
                     /locus_tag="PLS64_05945"
     CDS             complement(3089..7156)
                     /locus_tag="PLS64_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL43406.1"
                     /translation="MKKRSMKRALSAVLTLCMALPGASVAFGSASAATVSDDSSTSAS
                     STAAGSLVEDDGFTWDNANVYFLLTDRFKNGNTANDHSYGRTLDKDGKPLDGWDTNPG
                     TFHGGDFAGVTQSIEEGYFDNLGVNAIWISAPYEQTHGYCDSGKGFAHYSYHGYYVLD
                     YTETDANFGTKEEFKTLVDTAHEHGIRVIMDIVMNHTGYNTVADMEQFGFGTLLDGAL
                     DYKYKLTDVSDVNDHIDYKTSEEDWGKWWSNDWIRSGLPGYTEGAGGDLTMSLSGLPD
                     FRTEQTKDVTIPPILKTKWTQEGTYAQKLAKYGESNTVTGYLSTWLSEWVREYGVDGF
                     RCDTAKHVDKASWNQLKQACVSALREWRSNNKGKAGADWKEDFWMTGEHWDHGVGYDT
                     YYSEGGFDSMINFAVTGGGALASGSVANTYQGYADQINTKEGFNVLSFMSSHDETLTR
                     GDENTMLYNAAAFLLLPGGVQIFYGDETNRPLYPGVAFDGYGGSGHALRSDMNWNDMD
                     KTLLAQWQKVGQFRNKHVSIGAGANVKLSATSGVAFGRTYDKNGVTDQAAAVIGCNKN
                     SSVTVDVSTLWEDGSYVINTYDNSSSVVTDGKVTFNSGVNGTILMENADGQPLVSITG
                     EPKFYGTEQVTVSLKECDSAKVSVDGGNKFVVKDGDTFTIGQTAYKNDTIEVTVEATN
                     DKASSEAKFTFLKLGENDEKPTTSPTQSTTVPGPTASQTSKLTIKSTAGAPNVYAWTG
                     ASTALNGAWPGQKAAAVSGQANTYELTLPVSADKPFSVVLNNGSGSQSGDITGLYDGA
                     VLEIQNGNYASVKTVTNGNQQGGGDPEPAEGSVSVTIKPYSPSASYKIYAWNDTQKLT
                     GAWPGVALTEKDSDGNYVVKFDNVDEVSIILSSGSGQTADITGVRDGATIEITNEGCT
                     TYKLTSKPIVVSPYESLKKEARKILAMTASDYTAESWANAQKVLKSAEAMIKAGEDAT
                     TAEDMNAMIADLKSAQKALVLAPATLTYAVAGKSVVSGVTASAAKVTVTVDGKTYTAT
                     ADDVTGAFTVATSALKGTSTIKIDATRNGVNGTYSYAMKNGNIESGFVPTSPTLPTQP
                     TTSTQPTTATQPTTATQPTAPTQPTTATEPTTAPAQKLTVNATSNYFGSASKTVSTDG
                     KKVTVLYKLNSNMGVVDGQWAVKYDSSKLKFNMADNKADGKQTIMPSQSNLIHNAYSN
                     VIKGIFTNPQVPTQYNGDTFISLTFEVIGSGTASVYLDTQYLTLGYVKGNKLNQASVV
                     NNSKVCDVTGIAGFEKVSVNASTVFVGDVTLGDVNMDGDITVFDATEVQKYVVQLVAF
                     TNDQFTAADVDHDSTITVKDATTIQKFVVKLISNF"
     gene            complement(7461..>7854)
                     /locus_tag="PLS64_05950"
     CDS             complement(7461..>7854)
                     /locus_tag="PLS64_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002849804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL43407.1"
                     /translation="ADIRDVRIEKTVSNDFNLNCDFRWICEAVSNIVKNCIEHTDGGY
                     VRISAEKNSMYSEITISDNGCGISPKDLPHIFERFYKAQNSSPSSVGIGLSLAKSIVE
                     KTGGYITADSKLGVGSTFKLRFLNVRLR"
BASE COUNT         2070 a         1706 c         1690 g         2378 t
ORIGIN      
        1 agattgtatg ggcaaatcga ctgccataca gtagcggcaa tcactgattg ccaatcgaaa
       61 cgattgtaca atcgtttcga cttaattcaa ttcatctgct tattgttatg caattatata
      121 ttcacatctg ttcgttttgg ttttacgacc aaaacgattg gcgttcgctg aacgccgcta
      181 caattgagaa acggctttct ttgcaccaaa atatagggat taaacgcatg aaatcgcata
      241 aaaataaaaa ggtgtaataa acacttatcg gggcagagaa attccctgcc cccgattatg
      301 tatgcaaatc aaagatttaa ttatttgata aggcctttct ttgcacgaac cttgattgga
      361 agaccaaaga ggttgatgaa gcctgcactg tcgttctggt cataaacctg atcttcgtca
      421 aaagtagcaa tttcctcgtc gtagagtgaa tacgggcttg ttacgcctgc atcaatgatg
      481 ttgcccttat agagtttaag ttttacatca cctgttacat actcctgagt gctgtcaacg
      541 aaagctgaga gagcctcacg gagaggtgtg taccacttgc cgtcataaac gagctctgca
      601 aaagcatttg caacattctg cttgtagtgg agtgtgtcct tgtcaaggca gatttcctca
      661 agctttgcgt gagcagcata gaggattgta ccgcccggag tttcataaac gccacgagcc
      721 ttcataccaa caagacggtt ctcaacgata tctgtgatac cgacgccgtt tctgccgccg
      781 attttgttga gttccttaat gagttcaacg ctgtcgatag cctcgccgtt aagctttgtg
      841 ggaacaccct tttcaaatgt aagagttaca tattctgcct tatcgggagc ctgctcaggt
      901 gaaacaccga gttcaaggaa gccttcctta ttgtacatcg gctcatttgc aggattctca
      961 aggtcaagac cttcgtggct caagtgccag agattcttat cctttgagta gtttgtttca
     1021 cggttaatct tgagcggaat attgtgcttt tcagcatatt caatctcatc ttcacggctc
     1081 ttgaattccc atgttctcca aggagcgata atcttcatgt cgggagcgta agctttgata
     1141 gcaagttcaa aacgaacctg atcgttaccc ttgcctgtac agccgtggca aattgcgtca
     1201 gcaccttcgg cttttgcaat ttcaacgagt ctttttgcaa tgataggacg ggcaaaagat
     1261 gtgccgagga ggtacttgcc ctcgtaaaca gcacctgctt ttactgtggg aatgatatat
     1321 tcgtcaacaa attccttgtt gaggtcttca atgtaaagtt ttgatgcgcc tgttttgata
     1381 gccttttctt caagaccgtc aagctctgta ccctgaccaa catcacccga aacagcgata
     1441 acttcgcagt tgtcgtagtt ttctttaagc cacggaatga tgattgaagt atcaagaccg
     1501 cctgaatatg caagaactac ctttttaatc ttattgtcag ccatttttga ttcctccgga
     1561 atttggaaac actttatgtc ggtgtttcct ttaattaatt tttattactc ttacattata
     1621 cactctgttt tcaaaaaatc aagtgttttt tgaataaata ttcaatgttt tttcaaattt
     1681 gcatttttgg gtacaaattg accgaaatct gctttatttt cgctgtatta tcagtgtttt
     1741 tggtggttga tatgtcacat tcccctcgaa atttctgaaa aatgcatata tgcacaaaaa
     1801 atgaatattc attttattca tccatttatg catattcata catacatata catccatttt
     1861 gcatattttt cattttgtga aaaccgctga aatcacttca aaaacctgtt gacaccatat
     1921 gcgataccat atataatatt attcggaaat acgaaaacag gcattaagac aaattgttga
     1981 aagtgaggcg aaagatgatg aaaacactta acgattatct tgcaatgtca tatcgtatgg
     2041 aaattgtcga ggacaggaac gagggcggtt ttgttgtatc atttcccgag cttccgggtt
     2101 gcatagcctg cggtgatacg attgaatcgg ctgttgcaaa tgcagagaac gctaaaaaag
     2161 cctggtttga agccgccttt gaaagcggag ttgtaattca cgaacccgac agtcttgaag
     2221 aatattcggg acaattcaag ctgagaatcc cgaaaagtct gcaccgctca cttgccgaac
     2281 attcaaaaaa agagggcata agtatgaatc aatactgtgt ctatcttctg gcaaaaaatg
     2341 atgcagtcta ttcaaaatag aattttgaaa gcatattnnn nnnnnnnaaa acacaattgt
     2401 tgaaaggaag atctatatgt cagcattcaa agagataaat ccgaaagaaa ttgttgaaag
     2461 tccgtttaag cttatcggtg atgattgggc gcttgttacg gcaggcgaca gggaaaaatt
     2521 caacacaatg acgataagct ggggcggtgt aggcattatg tggggtaagc ccgttgtatt
     2581 cacctttatt cgtccgcaga gatatacatt tgcgtttatg gagaacggcg accgctatac
     2641 aatgagcttt tttgatgaaa agtacagaga tgccctcaaa ttctgcggtt caaagagcgg
     2701 caaggattat gacaaggtaa aggaaacagg tcttactcct gcgttcacgg aaaacggctc
     2761 tgtatatttt gaagaggcaa agcttgttct tgaatgcaaa aaaatgtacg cacaaagtct
     2821 taatgccgaa agcattaccg accgtgaaag cgttgacaag tggtatgaca acgacttcca
     2881 taaaatgtac atcagcgaaa ttacaaaggt tcttgtaaaa gtataatcgg taattttaaa
     2941 ttcatcatga caaaatggca gacaatcaca cggttgtctg ccattttcta tttaaaaaac
     3001 tgtacaaaaa atgtccctca cattttgtga gggacattcc ttactatatc gactaaacgg
     3061 aacgatatgt caattgcttt aaaacttatt aaaagttgct gatgagtttt acaacaaact
     3121 tctgaatagt agtagcatcc ttaactgtga ttgtgctgtc atggtcaaca tcggcagcag
     3181 taaactggtc gtttgtgaat gcaacgagct gaacaacata cttctgaact tcggttgcat
     3241 caaaaactgt gatatcgccg tccatgttaa catcaccaag ggttacatcg ccgacaaata
     3301 ccgttgatgc gtttactgat actttttcaa agcctgcaat gcctgttaca tcgcaaacct
     3361 tgctgttatt tacaacagat gcctgattaa gcttgttgcc ctttacataa cctaatgtaa
     3421 ggtactgtgt gtcaagatat acgcttgcag tacctgaacc gataacctca aatgtgagtg
     3481 agataaatgt atctccgttg tactgagtag gtacctgtgg gttagtaaat atacccttaa
     3541 ttacattgct gtaagcattg tgaataaggt ttgactgtga aggcatgatt gtctgcttgc
     3601 cgtctgcctt gttatcagcc atgttgaatt tgagcttgct tgaatcatac ttaacagccc
     3661 actgtccgtc aacaacgccc atgtttgagt tgagcttgta gagaacagtt accttcttgc
     3721 cgtcagtgct tactgtcttt gatgcagaac caaagtaatt tgaagtagcg tttactgtga
     3781 gcttctgagc aggagcagta gtcggctctg tagctgttgt aggctgtgta ggtgcagtcg
     3841 gctgagtagc tgttgttggc tgtgtagctg tagtcggctg agttgaagta gtaggctgtg
     3901 tcggaagtgt aggagatgta ggaacaaatc cgctttcaat gttgccgttc ttcatagcat
     3961 atgaatatgt gccgtttaca ccgtttcttg ttgcgtcaat cttgattgtg cttgttccct
     4021 tgagagctga tgttgcaact gtgaatgcac ctgttacatc gtctgcagta gcagtatatg
     4081 tcttaccgtc aactgtaaca gttacctttg cagcgctggc tgtaacacct gatacaacgc
     4141 tcttgcctgc aactgcatat gtgagtgttg caggagcaag tacaagtgcc ttctgagctg
     4201 acttgaggtc tgcaatcata gcattcatat cttcagctgt tgtagcgtct tcgcctgctt
     4261 taatcatagc ctcggctgac ttgagaacct tctgagcatt tgcccagctc tctgctgtgt
     4321 agtctgatgc tgtcatagca agaatctttc ttgcttcttt cttgagtgat tcataaggtg
     4381 aaacaacgat tggctttgat gtaagcttgt atgttgtaca accttcgttt gtaatttcga
     4441 ttgtagcgcc gtctctaacg cctgtgatat ctgctgtctg acctgaaccg ctgctgagaa
     4501 taatgcttac ttcgtcaaca ttatcgaact taacaacata gttgccgtca gaatcttttt
     4561 ctgtaagagc aacgcccggc catgcgcctg tgagcttctg tgtatcattc caagcataga
     4621 ttttgtagct tgcagatggg ctgtaaggct tgattgttac ggaaacagaa ccctctgcag
     4681 gctctggatc gccgcctccc tgctgattac cgttggttac tgtcttaacc gatgcgtagt
     4741 tgccgttctg aatttcgaga acagcaccgt cataaagacc tgtgatatcg cctgactgtg
     4801 agccgctgcc gttgttgaga acaacgctga atggcttgtc ggctgaaaca ggaagtgtga
     4861 gttcatatgt gtttgcctga cctgaaacgg ctgctgcctt ttgacccggc catgcaccgt
     4921 tgagtgctgt gcttgcacct gtccaagcgt aaacattcgg agcgcctgct gtgctcttga
     4981 ttgtaagctt tgatgtctga cttgctgtcg gaccgggaac agttgtgctc tgtgtcgggc
     5041 tggttgtagg tttttcgtcg ttttcgccga gcttgaggaa tgtgaattta gcctctgagc
     5101 ttgccttgtc gtttgtagcc tcaacagtta cttcgattgt atcgttcttg taagctgtct
     5161 gaccgattgt gaatgtgtcg ccgtccttaa caacaaactt gttaccgccg tcaacagaaa
     5221 cctttgcact gtcgcattcc ttgagagaaa cagtaacctg ctctgtgccg tagaacttcg
     5281 gctcgcctgt gattgataca agcggctgac cgtcagcatt ttccataagg attgtgccgt
     5341 ttacaccact gttgaaagtt accttaccgt cagtaacaac tgaagatgag ttgtcataag
     5401 tattgattac atagctgccg tcttcccaaa gagttgaaac atcaacagtt acgcttgaat
     5461 ttttgttaca gccgattact gcagctgcct gatctgttac gccgttctta tcgtatgtac
     5521 ggccgaaagc aacaccgctt gtagctgaga gcttaacatt tgcgccggca ccgatagata
     5581 cgtgcttgtt acggaactga ccaaccttct gccactgagc aagaagagtt ttgtccatgt
     5641 cgttccagtt catatcactt ctgagtgcat gacccgatcc gccgtaaccg tcaaatgcaa
     5701 cgccgggata gagaggacgg ttggtttcgt caccgtagaa aatctgaact ccgcccggga
     5761 gaaggaggaa tgcagccgca ttgtaaagca ttgtgttctc atcgccacgg gtaagtgttt
     5821 catcgtgtga tgacataaat gaaagaacgt taaagccttc ctttgtgttg atctggtctg
     5881 catagccctg atatgtgttt gcaacactac cgcttgcaag agcaccgccg cctgtaactg
     5941 cgaagttaat cattgagtcg aagccgccct cggaatagta tgtgtcgtag cctacaccgt
     6001 gatcccaatg ctcgcctgtc atccagaaat cttctttcca gtcagcacct gctttgccct
     6061 tgttgttgga tctccactct ctgagagcag aaacacaagc ctgcttgagc tggttccaag
     6121 aagccttatc aacatgcttt gcagtatcgc aacggaaacc gtcaacaccg tactccctaa
     6181 cccactctga aagccaggtt gaaaggtagc ctgttactgt gtttgattca ccgtattttg
     6241 caagcttctg agcatatgta ccttcctgag tccactttgt tttgaggatc ggaggaattg
     6301 taacatcctt tgtctgctct gttctgaagt cgggaagtcc gctgagtgac attgtaagat
     6361 caccgccggc accctctgta tatccgggaa gaccgctgcg gatccagtca ttgctccacc
     6421 atttgcccca gtcttcttct gatgttttat agtcaatgtg gtcgtttaca tcacttacat
     6481 ctgtgagctt gtatttgtag tctaacgcac cgtcaaggag agtaccgaag ccgaactgct
     6541 ccatatctgc aacggtgttg tagcctgtgt ggttcattac gatatccatg ataactctga
     6601 taccgtgctc atgcgctgtg tcaacgagag ttttgaactc ctcttttgta ccgaagtttg
     6661 catcggtttc ggtatagtca agaacatagt agccgtgata tgagtagtga gcaaagccct
     6721 tacctgaatc acaatagccg tgagtctgct catacggagc tgaaatccaa attgcgttta
     6781 caccgaggtt atcaaaataa ccctcttcaa ttgactgagt aacacctgca aagtcgccgc
     6841 cgtggaatgt accggggttt gtatcccagc catcaagcgg tttgccgtcc ttgtcaagtg
     6901 ttctgccgta tgaatggtca tttgcggtgt ttccgttctt aaaacggtcg gtaagcagga
     6961 aatacacatt ggcattatcc catgtaaagc cgtcatcttc aacaagtgaa cctgcggcag
     7021 ttgatgatgc agatgtgctg ctgtcatcgg aaactgttgc agcacttgcc gaaccaaatg
     7081 ctacggaagc accggggaga gccatacaaa gtgtcaaaac ggcactgagc gctctcttca
     7141 tacttctttt tttcattgtt tgattactcc tttcaaagct tttaaaacgt agggtcaaac
     7201 atccctagac aatttgattt tatcacaaaa ctctcacaaa aactacacat aatcctttta
     7261 ttttgtaaaa atagcaagtt acacaatctt tacactggaa ttttatgcaa aattaccctt
     7321 gtcgctcttc tgtttttatt tataaagaca gtttcaattg cccccctgcc gaaaattaca
     7381 taaatacggt cgggaataat aagatttgta cttaacaaaa ctttattccc gaccgcaatt
     7441 ttcgcttttt aatttttatc ttatctgagt cttacattca aaaatctgag tttaaaggtt
     7501 gagccgacgc cgagtttgga atctgcggta atatatccgc ctgttttttc aacaattgat
     7561 tttgcaagcg acagacctat gccgacgctt gacggggatg aattctgtgc tttatagaac
     7621 ctctcaaaaa tgtggggcaa atccttcggg ctgattccgc atccgttgtc gcttatggtg
     7681 atttccgaat acatactgtt cttttcggca gaaattctga cataaccgcc gtcggtatgc
     7741 tcaatgcagt tcttaacgat atttgaaacc gcttcgcaaa tccagcgaaa atcgcaatta
     7801 agattaaaat cattactgac tgttttttca attctcacat cacggatatc ggca
//
LOCUS       DAOMVE010000087         7674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2505_length_7674_cov_3.378396, whole genome shotgun sequence.
ACCESSION   DAOMVE010000087 DAOMVE010000000
VERSION     DAOMVE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_7674_cov_3.378396"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..1079)
                     /gene="hslO"
                     /locus_tag="PLS64_05955"
     CDS             complement(189..1079)
                     /gene="hslO"
                     /locus_tag="PLS64_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRL43408.1"
                     /translation="MDKIIRCITSDGAIMAACVDASDIVFTAKKIHHLSRSATAALGR
                     LLCATSIMGDMLKQKDASVNLRVMGDGELGPVIAVGDSSGNVKGYVGNADCPTEYYEN
                     GKINVAKAVGRTGMLNVMRDYGSGDPYIGQVELVSGEIAEDIANYYATSEQIPTVCAL
                     GVLIDKEDGEVLLAGGLLIQLLPGADDTVIDKLEKNIAELEPVTTMLAKGMSILDICK
                     TALKGFEVEVLDENPVKYVCGCSKEKLENYFCTLSDDDIRSMVNEQGVAVATCHFCNK
                     KYVFTKDDLEKLIAEKNSKE"
     gene            complement(1085..1861)
                     /locus_tag="PLS64_05960"
     CDS             complement(1085..1861)
                     /locus_tag="PLS64_05960"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRL43409.1"
                     /translation="MPSYLSFARFYDGLMQDACYEKRCDYILKLAESFGHDMGITLDL
                     ACGTGTLTRLLKKRGVDVFGIDYSMEMLSEAMQSASEEGLDILFLRQKMQSIDLYGTI
                     NTCVCTLDSINHLTKIEDVAKTFDRVGLFMDDDGLFIFDVNTVYKHKNVLADNTFVYE
                     NDSVFCVWQNTPEDDNKVKIDLDFFEEENGVYYRSSESFSERAYTDDEIRKMLTDAGF
                     EVEAVYGDLTLESPKADEQRAIYVARMKKSRNKEFNESKG"
     gene            complement(1861..2163)
                     /locus_tag="PLS64_05965"
     CDS             complement(1861..2163)
                     /locus_tag="PLS64_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HRL43410.1"
                     /translation="MNSKLKNDSTDYLFKAILSLENEEDCYRFFEDLCTSAELKAMSQ
                     RLVVAKMLTEKKVYTEIVKETGASTATISRVKRALFDNDTYGYTLVLDRIKNGEKE"
     gene            complement(2368..3150)
                     /gene="sigG"
                     /locus_tag="PLS64_05970"
     CDS             complement(2368..3150)
                     /gene="sigG"
                     /locus_tag="PLS64_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HRL43411.1"
                     /translation="MQYGKVEICGVNTSQLKLLKESEKRELLNIIKNGSPAEKKKARD
                     KMINGNLRLVLSVIQRFTNRGENPDDLFQVGVIGLIKAIDNFNPTLDVRFSTYGVPMI
                     IGEIRRFLRDNNSVRVSRSMRDTAYRAMQIKEQLTNKNNREPTVEEIAKIMEMPKEQV
                     VLALEAIVEPVSLYEPVFSDGNDTIYVMDQIGDKNDDSTWLEEIAVKEAITNLSDREK
                     RILSMRFFRGKTQMEVAAEIGISQAQVSRIEKGALDTIKKNI"
     gene            3363..3740
                     /locus_tag="PLS64_05975"
     CDS             3363..3740
                     /locus_tag="PLS64_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRL43412.1"
                     /translation="MFTIDFTSRVPIYEQICNNVIRLASAGVFKSGDRLPPVRTVAKE
                     IGVNPNTVAKAYRTLEIDGYIYSTVGRGTFMTDKLTKDTAYRDMALKAFREATKNAEL
                     YSVPRQQLIDIIDAIYEGGGKND"
     gene            3733..4629
                     /locus_tag="PLS64_05980"
     CDS             3733..4629
                     /locus_tag="PLS64_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43413.1"
                     /translation="MIEVNHITKRFGKVTAVDDFTLDINRGSVLGIVGSNGGGKSTLL
                     RILSGVFDADSGEVKINGQNIYNNPSVKGECFFIPDFPYFSNSATLENTAYLYRSLYP
                     NWNENAFRQFCSVFPIDPDAKIIDMSKGMQRQAALILAISTCPRYLLLDEIFDGLDPV
                     VRRLVKKILIDNVSANDMTVVIASHNLRELEELCDRICLIHKGKLLLEREIDEIKLGL
                     RKVQVAFTEVPDNSFFEEINVINLWRNGNIFNLTIRGTEDEFMPKLEERKPLYISAVP
                     MTLEEIFISEMGAAGYDAENII"
     gene            4607..6784
                     /locus_tag="PLS64_05985"
     CDS             4607..6784
                     /locus_tag="PLS64_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL43414.1"
                     /translation="MTQKTSFKSRMKQAFSFFRSDLRACTPLLVVFGLISAVTVVICY
                     TICVVLGEDMTFTGALLSLNFLGVTNSGTGIIEIFQYSSANLIYLISIIFTIIYTVRI
                     YSYLHNKRKADLYGSLPIGRSTLFVTKTASAYLMSIIPTLFFLGVISIITVCTGNSVI
                     SELSSMFLKICLGTLASISFFGLIAICCGTMLNSVLMFIAVCVAYPLSAIFIKGIVVG
                     CFDGFYVGIFKDSIIMNALNPLAAYDGINIIYWLIFSVACIVLGAFLAKKRKAERAQS
                     AFAFHLPCYIIKVLVSFLAGMFLGVLFGSVNVLGGYAGFVFGFILASVPVYIIVHLIL
                     YRGFSKLIKTSIPLGALIIVSCVGVALCCFDAFGYNSYVPKTQDIKSAGFYDANSNYQ
                     AKGSAVGKNLKDMADDITDKDTIANILEAHYKLVDNRNYSVKNKFKNTWVSMFKDNVP
                     VFSEISKNPDYAFAYTLNNGNIVTRTYSSASAFEETESYLSSTYNELDTSDIIYSKGY
                     IKAYSPLVKADYKDINTFIVAGFDGKATDDKHPDFVSSKIIYTDDDGTVNYSKNYKNT
                     AEKIKEAFLKDLDNCSDEDFAKIGQMTEYADHALDVAYVNYGEYESFIESEPDAVCVI
                     AIATGDVNSYYDGIRNMDSYNGNTAARTQVVYTVPKSFKNTIKALQELGILNSKLYMD
                     KDYLPGDSTSYKEYTGEYYYDDYTSSSDLYDYTDDSYIDDSEY"
     gene            6820..7356
                     /locus_tag="PLS64_05990"
     CDS             6820..7356
                     /locus_tag="PLS64_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HRL43415.1"
                     /translation="MELYNGRPTDLTGREDREIRVYDLLDSLGIEYQRTDHGHADTME
                     ACNEIDAVLDVLICKNLFLCNRQKTDFYLLMMPGDKPFKTKELSKQIGSARLSFATPD
                     FMLEFLDIKPGAVRLMNDKDNRVRLLVDEDVLKGEYVGCHPCVNTSSLKIKTADVFGK
                     YLDAVHHTATTVKLIGED"
BASE COUNT         2168 a         1665 c         1517 g         2324 t
ORIGIN      
        1 gctctcccag ctgagctatg ctcccacatg cctttgtcgc tctatcgaag ttctgttgaa
       61 tttcgaatat ctctcagcga cagatattat catactacaa cttgttccaa atgtcaacac
      121 ttttttgaaa tttttttcat ttttatgagt gacatcacga acatttgcga caaggccttt
      181 aaaaatcttt attctttgct gtttttctct gcaatcagct tttcaaggtc atcctttgta
      241 aagacatatt tcttattgca gaagtgacag gttgcaacgg caacaccctg ttcattgacc
      301 atagaacgga tatcgtcatc gctgagggta caaaaataat tttcaagctt ttcttttgaa
      361 caaccgcaaa catacttaac agggttttcg tcaagcactt caacctcaaa gcctttgagg
      421 gctgttttgc agatatcaag aatactcata ccctttgcaa gcatagttgt aacgggttca
      481 agctctgcaa tattcttttc aagcttgtca ataacagtat cgtctgcacc cggaagaagc
      541 tgaataagca atccgcctgc aagcaaaacc tcgccgtcct ctttgtcaat cagcacaccc
      601 aaggcacaaa ctgtgggaat ctgttcgctt gttgcgtagt agtttgcaat atcctcggca
      661 atttcaccgc tgacaagctc aacctgacct atgtacggat cgcctgaacc gtagtcacgc
      721 ataacattta gcatacctgt tctgccgact gcttttgcaa cattgatttt gccgttttca
      781 taatattccg tcgggcagtc tgcattgccg acataaccct ttacattgcc gctgctgtcg
      841 ccgactgcaa taacgggacc gagttcaccg tcacccatta cacggaggtt gactgacgca
      901 tctttctgct tgagcatatc gcccataatt gaggttgcac acaaaagtct gccgagtgcg
      961 gctgtggcac tgcgtgaaag atggtgaatc ttctttgcgg taaaaactat gtcagaggca
     1021 tcaacgcaag ccgccataat cgcaccgtca gatgtaatac aacgaattat tttatccata
     1081 tcggttatcc tttcgattca ttaaactctt tgttgcgtga ttttttcatc cttgcaacat
     1141 aaattgccct ctgttcatcc gctttcgggc tttcaagggt aaggtcgccg taaacagcct
     1201 ctacttcaaa acctgcatct gtgagcattt ttctgatttc atcatctgta taggctcttt
     1261 ccgaaaagct ttcggacgaa cgataataca caccgttttc ttcctcaaag aaatcaaggt
     1321 cgatttttac cttgttatca tcttcgggag tgttttgcca tacgcagaaa acgctgtcgt
     1381 tttcatacac aaaggtatta tccgcaagaa catttttatg cttgtacacc gtgttgacat
     1441 caaaaataaa cagaccgtca tcgtccataa aaagtcccac acggtcaaat gtctttgcaa
     1501 catcttcaat ttttgtaaga tgatttatgc tgtcgagagt acaaacacag gtatttattg
     1561 taccatacaa gtcaatactc tgcatcttct gccttaaaaa caggatatca agcccttctt
     1621 cggaggcact ttgcattgcc tcgctgagca tttccatact gtaatcaatt ccgaaaacat
     1681 ccacgccccg ctttttcaaa agtcgggtaa gagttcccgt tccgcaggca aggtcaagcg
     1741 ttatgcccat atcatgaccg aaactttcgg caagctttaa aatatagtca cagcgttttt
     1801 catagcaggc gtcctgcata agtccgtcat aaaatcgggc aaatgacaaa tagctcggca
     1861 ttactctttt tctccgtttt taattcggtc aagcacaagc gtgtagccgt atgtatcgtt
     1921 gtcaaacaac gctcttttca ctcggctgat agttgcggtt gacgcacctg tttccttaac
     1981 aatttcggta tatacttttt tttcggtaag catttttgca acaacaagac gctgtgacat
     2041 ggctttaagc tctgcacttg tgcataaatc ttcaaaaaag cgatagcaat cttcctcgtt
     2101 ttcaagagaa agaattgcct tgaataaata gtcggtagaa tcatttttca gctttgaatt
     2161 caaaaccgtt tcctccttaa ttttgtttcc gtaaattttg caaacaatat gttttcatac
     2221 actaaaattt taacacacaa aagtacgaaa gtaaagtaaa aactgctttt tcgtcttttt
     2281 ttacgaaatt tgcctgtgat tttttacaca taaatttatg caaacacaaa acagccgtcc
     2341 gactgaacag ctgttttgca cttacggtta aatgtttttc tttatagtat cgagagcgcc
     2401 tttttcaata cggctgacct gtgcctgtga aattcctatt tcagccgcaa cctccatctg
     2461 agttttgcca cggaaaaacc tcattgaaag aatccgcttt tccctgtccg aaagatttgt
     2521 aattgcctct tttacggcaa tttcttcaag ccatgttgaa tcgtcattct tatcgcctat
     2581 ctgatccatt acatatatag tatcgtttcc gtccgaaaac acgggttcat aaagcgacac
     2641 cggctcgaca attgcctcaa gcgcaagcac cacctgttcc tttggcatct ccataatttt
     2701 ggcaatttct tcaacagtcg gctcacggtt atttttattt gtgagctgtt cttttatctg
     2761 cattgcacgg taagccgtgt ccctcattga gcgtgaaacc ctgactgagt tgttgtcacg
     2821 cagaaaccgt ctgatttcac cgattatcat aggcactccg taggtgctga atcttacatc
     2881 aagcgtcgga ttaaaattgt caatcgcctt gataagcccg attacaccca cctgaaacaa
     2941 atcgtcggga ttttcgcccc tgtttgtaaa tctttgaatt acggacagca caagccgcaa
     3001 atttccgtta atcatcttgt ctcttgcctt tttcttttcg gcaggagaac cgtttttgat
     3061 tatattaagc agttcccgtt tttcgctttc tttgagcagt ttaagctgtg atgtgtttac
     3121 tccgcagatt tcaacttttc cgtattgcaa agaattaccc ccgaatctga attttaatcg
     3181 gttttgtctt tacaatagtt attgacggtt ttatcgcttt tatactttga gtaatttttt
     3241 taaaaagttt tctaaacttt aaaattgtac attgacaatg gtacaatcaa atggtacaat
     3301 ttaagtgtag aattagccat gttatacaaa ttcgcatata aatcattaaa aggaggacgg
     3361 ctatgtttac aattgatttc acaagccgtg tgccgattta cgagcagatt tgcaacaatg
     3421 taatcagact tgcatcggcg ggtgtgttca aatcgggtga cagactgccg cctgtacgaa
     3481 cggttgcaaa agaaatcgga gtaaacccga ataccgttgc aaaggcatac cgcacgcttg
     3541 aaattgacgg ctacatttac tcaactgtcg gcagaggaac atttatgacc gacaagctga
     3601 caaaagacac ggcatacaga gatatggctc tgaaagcatt cagagaagcc accaaaaatg
     3661 cagaactgta cagcgttccc cgccaacagc ttattgacat tattgacgcc atatacgaag
     3721 gaggcggtaa gaatgattga ggttaatcac ataacaaaaa ggttcggtaa ggtaactgcc
     3781 gttgatgatt ttacgctcga tataaaccga ggctccgtac tcggcattgt cggaagcaac
     3841 ggcggcggaa aatcaacact tctgagaatt ttgtcaggtg tgtttgatgc tgacagcggt
     3901 gaagttaaaa ttaacggtca gaacatttat aacaatccgt ctgttaaggg cgaatgtttt
     3961 tttattcccg attttccgta tttttcaaac agtgcaacac tcgaaaacac ggcatatctt
     4021 taccgcagtc tttacccgaa ttggaacgag aacgctttca gacaattttg ctccgtgttt
     4081 cctattgacc ctgatgcaaa aataattgat atgtcaaaag gtatgcaaag acaggcggct
     4141 ttgatattgg cgatttcaac ctgtccgaga tatcttttgc ttgatgaaat ttttgacgga
     4201 ctcgatccgg ttgtccgccg tcttgtaaag aaaattctca ttgacaatgt ttccgcaaac
     4261 gatatgaccg ttgtcattgc gtcacacaat ctgcgtgagc ttgaagaatt gtgcgacaga
     4321 atctgcctta tccacaaggg caagttgctt cttgagcgtg aaattgacga aatcaagctt
     4381 ggactccgca aggttcaggt tgcgttcacg gaagttcccg acaattcgtt ctttgaagaa
     4441 atcaatgtta tcaatttgtg gagaaacgga aatatattca accttacaat cagaggaacg
     4501 gaagatgagt ttatgccaaa gcttgaggag cgtaaacctc tttacatttc agccgttccg
     4561 atgacattgg aggaaatttt tataagtgaa atgggggcag caggttatga cgcagaaaac
     4621 atcatttaaa agcagaatga aacaggcatt ttcattcttc cgcagtgacc tgagagcctg
     4681 tactccgttg cttgtggtat tcggacttat ctcggctgtc acggttgtaa tctgctacac
     4741 aatatgcgtt gtgcttggcg aggatatgac ctttacaggt gcattgttaa gccttaactt
     4801 tctgggagtt acgaactcag gcacaggcat aatcgagatt ttccagtatt catcggcaaa
     4861 cctcatttat cttatttcaa taatatttac aatcatctac acggtgcgaa tttattcgta
     4921 tctgcacaat aaacgcaagg ctgatttgta cggttctctg ccgattggca ggtcaacgct
     4981 gtttgtcaca aagacagctt cggcatacct catgtcaatt atcccgacat tattcttcct
     5041 cggagtgatt tcaattatca cggtctgcac aggaaattcc gtaataagcg agcttagttc
     5101 gatgttcctc aaaatatgcc tcggaacact tgcatcaatc tcgtttttcg gtcttattgc
     5161 aatttgttgc ggaacaatgc tcaactccgt gcttatgttc attgcggttt gcgtagctta
     5221 tccgctttcc gcaatcttca tcaaaggcat tgttgtgggt tgttttgacg gcttttatgt
     5281 cggcattttc aaagacagca ttatcatgaa cgcactcaat ccgcttgccg catatgacgg
     5341 aatcaacatt atttactggc tgattttctc ggttgcttgt attgttctcg gtgcttttct
     5401 tgcaaaaaag agaaaagcgg aaagagcaca gtcggcattt gcattccacc ttccgtgcta
     5461 cataatcaag gtgcttgttt cattccttgc aggtatgttc ctcggtgtgc ttttcggctc
     5521 ggtaaatgtt ctgggcggat atgcgggctt tgttttcgga tttattcttg caagcgttcc
     5581 cgtctacata atcgttcatc ttattttgta cagaggcttt tcaaagctta tcaaaacttc
     5641 gattccgctc ggtgcgttaa ttattgtaag ctgcgtcggt gttgctctct gctgttttga
     5701 cgcattcggc tacaacagct atgtaccgaa aactcaggat atcaagagtg ccggattcta
     5761 tgacgcaaat tcaaactatc aggcaaaagg cagtgctgtc ggcaaaaatc ttaaagatat
     5821 ggctgacgat attacagaca aagacacaat cgcaaatatc ttggaggcac attacaaact
     5881 tgttgacaac cgcaattatt cggtaaagaa caagttcaaa aacacttggg tttcaatgtt
     5941 caaggacaat gttcctgtat tcagcgagat ttcaaaaaat cccgattacg catttgccta
     6001 cacactcaac aacggtaaca tcgttacaag aacatacagc tctgcatcgg cttttgaaga
     6061 aacagagagc tatctttcaa gcacttataa cgaacttgac acatccgaca tcatttattc
     6121 aaagggctat atcaaggcat acagtccgct tgtaaaagct gattacaagg atattaacac
     6181 atttatcgtt gcaggctttg acggcaaagc aactgacgat aagcatcctg attttgtatc
     6241 aagcaaaatt atttatactg atgatgacgg aacagtaaat tattctaaaa attacaaaaa
     6301 tacagccgaa aaaattaaag aggcttttct caaagacctt gataattgca gtgacgagga
     6361 ctttgcgaaa ataggtcaaa tgaccgagta tgcagatcat gcgcttgatg ttgcctatgt
     6421 caattacggt gaatacgaaa gctttataga atccgaaccc gatgctgtat gtgtgattgc
     6481 gattgcaaca ggtgatgtta attcgtatta cgatggaatt agaaatatgg attcttacaa
     6541 cggcaatact gccgcaagaa cacaggttgt ttatactgtt cccaaaagct ttaaaaacac
     6601 tataaaagcg ttgcaggagc ttggtattct gaacagcaag ctgtatatgg acaaggatta
     6661 tctgccgggt gattcaacat cttataagga atacacaggc gaatattact acgatgatta
     6721 cacctcctca tccgatttgt atgactatac agacgattct tatattgacg acagcgaata
     6781 ttgatacagg cattacacta caggaaagca ggattttaaa tggaacttta caacggacga
     6841 ccaaccgact taacaggcag agaagaccgt gaaatcagag tatatgacct tctcgacagc
     6901 cttggaattg aatatcagcg aaccgaccac ggacacgctg acacaatgga ggcttgcaac
     6961 gagattgacg ctgtacttga tgtgctgatt tgcaagaatc tttttctgtg caacaggcag
     7021 aaaaccgact tctatcttct tatgatgccg ggtgacaaac cttttaaaac aaaggagctg
     7081 tcaaagcaaa tcggaagtgc aagactttcg tttgcgacac ccgattttat gctcgaattt
     7141 ctcgacatca agccgggtgc ggtgaggctt atgaatgaca aagacaaccg agtaagactt
     7201 cttgttgacg aggatgtttt gaaaggcgaa tatgtcggct gtcatccgtg tgtaaacacc
     7261 tcaagtttaa aaattaaaac agccgatgtt ttcggaaagt atcttgatgc ggttcaccac
     7321 accgcaacaa ccgtaaagct tatcggtgaa gattaagtgt tgaaatcatt aaagtattaa
     7381 acggcaacgg gataaatttt tccgttgccg tttcgttttt tacagaacat aactgccccc
     7441 gaacctttga aagattcggg ggcagttgtc tgaatattaa gaaaaatgag tgtcaattat
     7501 ttgatttact tttcaatctt tcttctgtag caaacgaaca ttgcaccgct tgcaacgagc
     7561 attacaagtg caaccgaaac agcagtcatc gggttgcctg tctgaactgc accgttgccg
     7621 cctgttgcag attttgttac tgtatccttt gttgcagtat cttttgttga agaa
//
LOCUS       DAOMVE010000088         7605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2536_length_7605_cov_3.340927, whole genome shotgun sequence.
ACCESSION   DAOMVE010000088 DAOMVE010000000
VERSION     DAOMVE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2536_length_7605_cov_3.340927"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..493
                     /locus_tag="PLS64_05995"
     CDS             50..493
                     /locus_tag="PLS64_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43416.1"
                     /translation="MIVLGMVVLSLMSALTFSVFSAGNVSDTIINISARSNNGGTPCR
                     SIWREKKDYTSVWCENKSTSGGSLSAWVQRTNNKNTSSVKTVDRYYGSWSYNGATKNM
                     KNLPKGTYYYLPNYVKEDGYKYATLGYIMNKEAVSYHIAWSPDSI"
     gene            614..1306
                     /locus_tag="PLS64_06000"
     CDS             614..1306
                     /locus_tag="PLS64_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43417.1"
                     /translation="MNKKITTVIILIIICASSIIVSILYLIKNDIIDINDIYTNADAK
                     NTQVANENSISSTENSAVESKSCVLLEEGAEYDTMYDDRTSIYVKLNNISISKNKAEL
                     PEFDTFEDWDEIKDSQGNITNEYSYVVLNITMMNKGNENVEVSLNSLLLHFGTDGGYN
                     EARSFNSSEKKTSKNYFVEKLEPGKKLNVNVAYVAEDKQLNEYKDQLYLMTVLSGNPL
                     GEGTMQVIKSEE"
     gene            1310..2149
                     /locus_tag="PLS64_06005"
     CDS             1310..2149
                     /locus_tag="PLS64_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43418.1"
                     /translation="MENDKMQGMIKFELKRSFKNRLFLISLLISLALVTWYSIERIPY
                     CVQLNSEFLNDENLMGFFEISYTNWIGSHNIYLQQNIFYMAIPILATLPFGSSFYDDI
                     NRGYARNVCVRAKKKYYLVAKYIAVFVSGGCVVAIPMVLSFLISSIFLPTMLPEASYT
                     FTNIYPAYKWADLFYVQPLLYTSIYIFITFLFSGVTASLSLFITFFLNRKFVTFVFPF
                     FIYIFSSFCLEMLGFDAYSIRNLVTTTSESGTTFSVTILFLIFFCLSFFPYYFIGAKK
                     DVF"
     gene            2139..2840
                     /locus_tag="PLS64_06010"
     CDS             2139..2840
                     /locus_tag="PLS64_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43419.1"
                     /translation="MFFKACRKTFVAMTYFKYLVVFCLLCSFVLFYFFAFLKKNMMDI
                     TDNICMADFVEAAGGHTTTIFFVPTAALLSTIFSEKNSNFTNAYIRFRFRNTLVVRNS
                     LKVLAFSVAFCILFLVAVLGMSRFFSRTVINWNSYESYYYLCEGCISNVGFAKVVMKT
                     FVCLLIPTIFFSELICAFGCIINKVFPFLVTVTTCIAVSFDGDWNIDFWLLIFGTIIV
                     FVLNLLFMKRKDFLN"
     gene            2762..3673
                     /locus_tag="PLS64_06015"
     CDS             2762..3673
                     /locus_tag="PLS64_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43420.1"
                     /translation="MALDFWDNYSIRTKFIVHEKKGFFKLKVRLFFRLLSHDITEGII
                     HNRKFFIFAALLFIFIDFVYWHNISLYFSDYNSVVDCGVLDFFMNVFAGCDPFDPEAN
                     KGVDIPITWFSFNVLPYLFVGLYITNDLQTSADTFILRVKSRYLWWLSKIVWCVVSST
                     LYYLLFFVISTAFTLFSGNFSLTQNSLITEEFLELSTYGKSMTEIFISSVLLPWMITT
                     CHMTFDAIISITFGPVVAFLMIVCLMTTSVFYCSEFLPFNFSMLIRTDFCAINNISIY
                     TELEVAFLIIVICLFLGLPIIKRKNII"
     gene            3688..4344
                     /locus_tag="PLS64_06020"
     CDS             3688..4344
                     /locus_tag="PLS64_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL43421.1"
                     /translation="MNIEICNYTKKLSKNLVLENINLSLSGGKIYGFVGKNGSGKTML
                     MRAICGLILPTSGFVKINNKIIGKDISFPDSVGALIENPGFISEYSGFQNLKILAQIQ
                     KKIDDKKIVDTMRMVGLDPNEKKSFRKYSLGMKQKLGIAAAMMEDPQILVLDEPFNAL
                     DEASCENVKKMILSAKVPDRVVLLSCHDKNQINALCDETIELAQGKIIRRDSLYEKEK
                     "
     gene            4328..4906
                     /locus_tag="PLS64_06025"
     CDS             4328..4906
                     /locus_tag="PLS64_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43422.1"
                     /translation="MRKKNNLKVIVTLLIVTVLCICGVIRFFSINSKYPQNEKVVYKM
                     GDSFVCQNAKFTITDTYMLKRMDIMNDDDLSEYLEENSDYLKSEDLNLGLIKIIISNT
                     TDDEITVDLTAFHIESGAFSLQFYYPLVMYYNDCGMYIKLSKGEQKTFIAPVPISSEQ
                     FLNYDSTDIRNRTYYLVSSLYPQKIMSEVKFN"
     gene            complement(5021..5275)
                     /locus_tag="PLS64_06030"
     CDS             complement(5021..5275)
                     /locus_tag="PLS64_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43423.1"
                     /translation="MKRNPLPFLISPHSEIDGKLNDEINVQAAREYARRRCNQTQTRN
                     TMRHEHKRKNNSLPLKGNLNIFSSLNKKRITTPCCIAVLR"
     gene            complement(5721..5768)
                     /locus_tag="PLS64_06035"
                     /pseudo
     CDS             complement(5721..5768)
                     /locus_tag="PLS64_06035"
                     /inference="COORDINATES: protein motif:HMM:NF025645.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5758..6120)
                     /locus_tag="PLS64_06040"
     CDS             complement(5758..6120)
                     /locus_tag="PLS64_06040"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRL43424.1"
                     /translation="MRKNKKEFTHPFSWSGYVVRSILAKQEYCGDTVNFRTHKESYKD
                     KSSVKNPQENWLIFNDTHEAIIDRETWELAQKLTKTPRRVDTTGVANPLTGLVYCADC
                     GAKMYNHRFFRAYYADDK"
     gene            complement(6562..>7605)
                     /locus_tag="PLS64_06045"
     CDS             complement(6562..>7605)
                     /locus_tag="PLS64_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRL43425.1"
                     /translation="PVSGWMLDYFYKFATGTFKSGMNSEQVSGVFNDLMASPVEMIIW
                     LAIIVVFGFFVCSRGLQKGIEKVSKVMMVALLALIIILAINSMMLSNAGEGLSFYLVP
                     DFGKVKDVGLGKVITSAMSQAFFTLGLGIASMEIFGSYMNKDRTLYGEAVQICALDTF
                     VAIVAGLIIFPACFSFGVQPDQGPALIFVTLPNVFVNMTGGRIWGTLFFLFMTFASFS
                     TVIAVFENLVAFLTDTFGMSRTKASIINGIIMFFACLPCIFGFNIWSDFNILGKGVLD
                     LEDFVVSNLLLPIGAMVYLLFCTTKFGWGFDNYFEECNTGKGLKLSRVLKPYLKYALP
                     VLIVIVFVQGFIG"
BASE COUNT         2334 a         1273 c         1439 g         2559 t
ORIGIN      
        1 cggtgtttta atatttcaaa tgaggtaaaa gatgaagaaa gtaaaaaaga tgattgtgtt
       61 gggtatggtc gttctttctt tgatgtctgc tcttactttt tcagtattct ctgccggtaa
      121 tgtttcagat acaattatca acattagtgc gagaagtaac aacggaggta ctccgtgcag
      181 atctatttgg cgtgagaaga aagattatac atctgtttgg tgcgaaaaca agagcacatc
      241 aggaggttct ctgagtgctt gggttcagcg tacaaataat aagaatactt cctccgtaaa
      301 aactgtagac agatattacg gttcatggag ctataatggg gcaactaaga atatgaaaaa
      361 tttaccaaag ggtacatatt attatttgcc taactatgtt aaagaagacg gctataaata
      421 tgctactttg ggctacatta tgaataagga agctgtgagt taccacatag catggagtcc
      481 tgacagcata taaaatattt gtggctttgt aagttatgct ctgtgccttt taatgaagtt
      541 ttgactttat atatgcgcgc agagcatgac ttttagactg cggtagggag ttgcttatag
      601 aggtgtttat atattgaata aaaaaataac aaccgtaatt attttgatta tcatctgtgc
      661 ttcatctata atagtttcca tcttatactt gataaaaaat gatattattg acatcaacga
      721 tatatacact aatgctgatg caaaaaacac acaggtcgca aatgaaaatt ctatttcttc
      781 cacagaaaat tcagcagttg aatcgaaaag ttgtgttctg cttgaggaag gtgctgaata
      841 cgatactatg tacgacgata gaacaagcat ttatgttaaa ttaaataata tcagcatttc
      901 aaaaaataag gctgaacttc ccgaatttga cacatttgag gactgggatg aaattaaaga
      961 tagccaagga aatattacaa atgaatattc atatgtagtg cttaacatta caatgatgaa
     1021 taaaggaaat gaaaatgtgg aggtatcatt gaacagctta cttttgcatt ttggtacaga
     1081 cggtggatat aacgaagcac gaagttttaa ttcaagtgag aaaaagactt caaaaaatta
     1141 ttttgtagaa aaacttgaac ccggtaaaaa attgaatgtt aatgtagcat atgttgctga
     1201 ggacaaacaa cttaatgagt acaaagacca actttatttg atgacagtgc tctcaggcaa
     1261 tcctttagga gaaggaacta tgcaagtcat caaatccgaa gaataataaa tggagaatga
     1321 taaaatgcaa ggaatgatta aattcgaact gaaaagaagt tttaaaaaca ggcttttttt
     1381 aatttctttg ctaatttcat tagcattggt aacatggtat tcgattgaaa gaataccata
     1441 ctgtgtgcag ttgaattctg aatttttaaa tgatgaaaat ttaatgggat tttttgaaat
     1501 ttcatataca aactggattg gcagtcataa tatctatctt cagcaaaaca ttttttatat
     1561 ggctattcca attctcgcca ctttgccatt tggtagttcc ttttatgatg atataaacag
     1621 gggatatgca aggaatgtgt gcgttcgtgc caagaaaaaa tattatcttg tggcaaaata
     1681 catagctgtt ttcgtaagtg gtggttgcgt ggttgctatt ccaatggttc ttagttttct
     1741 catttcttca atatttttac caacaatgct tcctgaagca tcttatacat ttactaacat
     1801 atatcctgcg tataagtggg ctgatttatt ttatgtgcaa ccgttattat atacatcgat
     1861 atatattttt atcacatttt tgttttcggg agtaacagca agtttgtcat tgtttattac
     1921 attcttttta aacagaaaat ttgtgacttt tgtttttcct tttttcattt acattttttc
     1981 atcattttgt cttgaaatgt tgggatttga tgcctacagt atcagaaatc ttgtcacgac
     2041 tacaagtgaa agtgggacta ccttttctgt aacaatttta tttttgattt tcttttgcct
     2101 ttcatttttt ccgtattatt ttattggagc gaaaaaagat gttttttaaa gcgtgtagaa
     2161 aaacttttgt tgcaatgaca tattttaaat atttggttgt tttctgttta ctctgcagtt
     2221 ttgtattgtt ttactttttt gcttttttaa aaaaaaatat gatggatata acagacaaca
     2281 tatgtatggc agattttgtg gaagccgccg ggggtcacac gacaactatc ttttttgtac
     2341 caaccgccgc attactttca acgatatttt ccgaaaaaaa cagtaacttt acaaatgcct
     2401 atattcgctt tagatttaga aatacactgg ttgtcagaaa tagtttgaaa gttcttgctt
     2461 tttctgtggc tttttgcatt ttattcctcg tcgcagttct tggtatgtcg agattttttt
     2521 cacgaacagt aattaattgg aattcatatg aaagctatta ttatctgtgt gaaggatgca
     2581 taagcaatgt aggctttgca aaagtagtga tgaaaacttt tgtatgtctt ttaattccta
     2641 caatattttt ttctgaatta atatgtgctt ttggctgtat aataaacaaa gtatttccgt
     2701 tcttagtgac ggtcacaaca tgtattgcag tttcatttga tggtgattgg aatattgact
     2761 tttggctctt gatttttggg acaattatag tattcgtact aaatttattg ttcatgaaaa
     2821 gaaaggattt tttaaattga aagttcgtct tttttttcgt ttgctttcgc atgatataac
     2881 agagggaatc atacataatc gtaaattttt tatttttgct gcgttgctgt tcatatttat
     2941 tgatttcgtg tattggcata atattagctt atattttagt gattataatt cggttgtaga
     3001 ctgcggtgtt ctggattttt ttatgaatgt ttttgccgga tgtgaccctt ttgatccgga
     3061 ggcaaataag ggtgtagata ttcccataac gtggttttct tttaatgtac ttccttattt
     3121 gtttgtggga ttatacatta caaacgattt gcagacaagt gccgatacct ttattttgcg
     3181 ggtaaaatcc cgatatttat ggtggttgag taaaatagtt tggtgtgttg tttcttcaac
     3241 tttgtactat ttactgtttt ttgtaatttc gacagctttt acattgtttt ccggtaattt
     3301 ttcattaact caaaacagtt taataactga agaatttttg gagctaagca cttatggaaa
     3361 aagtatgaca gaaattttca tttcctcagt actgttacct tggatgatta caacttgtca
     3421 catgacattt gatgcaatta tcagtattac atttggacct gttgtcgcgt ttttgatgat
     3481 agtctgcctt atgaccacat ctgtgtttta ttgcagcgaa tttttgccgt ttaacttttc
     3541 aatgttgata agaactgatt tttgcgccat aaataatata tcaatatata cagaactgga
     3601 ggttgccttt ttaataatcg ttatatgttt atttttaggc ttgccaatta tcaaaagaaa
     3661 aaacattatt taaagtagga gagatttatg aatatagaaa tttgtaatta tacaaaaaag
     3721 ttatcaaaaa atcttgtact ggaaaatata aatttatcac tttccggagg aaaaatttac
     3781 ggttttgtag gtaagaacgg ttcggggaaa actatgctta tgcgtgcaat ttgcggactg
     3841 attttaccta ccagtggttt tgtaaaaata aataacaaaa tcataggaaa agacatatcg
     3901 tttcctgata gtgttggggc tttaattgaa aatccggggt tcatttctga gtactccggt
     3961 tttcaaaatt tgaagattct cgctcaaata caaaagaaaa tagacgataa aaaaatagtg
     4021 gatactatga gaatggtagg gcttgaccca aacgaaaaga agagtttccg aaaatattca
     4081 cttggtatga aacaaaaact tggaatagca gcagcgatga tggaagaccc tcaaatactt
     4141 gttttagatg aaccttttaa tgcgttggat gaagcttcat gtgaaaatgt caaaaaaatg
     4201 attttgtccg caaaagttcc cgatagagtt gtgttgctgt catgtcatga caaaaatcag
     4261 attaacgctc tttgcgacga aactatagaa ttagcgcagg gtaaaatcat caggagagat
     4321 agtctttatg agaaagaaaa ataatttaaa agttattgta acgctcctta ttgtaacagt
     4381 attatgtata tgtggtgtca ttaggttttt tagtatcaat tcaaaatatc cgcaaaacga
     4441 aaaagttgta tataaaatgg gcgattcatt tgtttgtcaa aatgcaaaat tcactataac
     4501 agatacatac atgcttaagc gtatggacat tatgaatgat gatgatttat cagaatattt
     4561 ggaagaaaat tctgactatt tgaaaagtga agatttgaat cttggcttaa taaaaattat
     4621 aatttccaat acaacagatg atgaaataac tgttgattta acagcttttc atattgaatc
     4681 aggtgctttt tcattacaat tttattaccc gctcgttatg tattacaatg attgtggaat
     4741 gtatattaaa ctttcaaaag gtgaacaaaa aacgtttatt gcacccgttc ctatctcttc
     4801 tgaacaattt ctcaattatg attccaccga tattagaaac agaacgtatt atttggtatc
     4861 atctttatat cctcaaaaaa taatgtcaga agtcaaattt aattaaattc aagatattta
     4921 aattggtcaa ataaataaca atgttggttt agcataatta gtgctgaatt acttggacat
     4981 tgaaaagttg cctcgttgta taatcatgac gaaaatctga ttaacgcaaa actgcaatac
     5041 agcaaggtgt cgtaatccgt ttcttgttca agcttgaaaa aatatttaaa tttcctttta
     5101 acggtaaact attgtttttt ctcttgtgtt cgtgccgcat tgtatttctc gtctgagttt
     5161 ggttgcaacg gcggcgggca tattcacggg cagcttgcac attgatttca tcattcagtt
     5221 tgccgtcaat ctcactgtgc ggcgatatta aaaacggaag tggatttctt ttcattttga
     5281 agtcccttcc gttcattttt cttttcgttt atctgcttta cggcggcttt ataccgcgcc
     5341 aaagtttcct ttacagactt tgaataatcc gttatgctcc acttgccggt ttgagtgtgt
     5401 cgaatatgat agttgatgat tatcagcctt tcacgcaggg ttggtggtga tctgttgact
     5461 gagcctgcac aatcttagca aagcattgct gtgcttggat tcaaatacct tgtcacagcc
     5521 cttgcagcta agtgaaaacg gctcgccaac cttgtgtttt ctcgccttta tcaactgata
     5581 gcattccctg ctttttagac gggctgtttc agctgcttct gccgcttaat ttcttcctcg
     5641 ctcagctgag gttgaggaat ttcaaacttg ccgatgaaat tgagatagat ttcaacctcc
     5701 tgcatacgtt ctccgtcaat ataatacgga gcgtgaacta aaattttgtc gataaactca
     5761 tttatcatcg gcgtagtaag ctcggaaaaa tcggtggtta tacatctttg caccgcaatc
     5821 ggcacaataa acaagacccg tcagcggatt cgccactccg gtggtgtcaa cacgtctcgg
     5881 cgtttttgtc agtttctgag caagctccca agtttctcgg tcaatgattg cttcgtgcgt
     5941 gtcattgaat atcagccagt tctcctgcgg atttttcact gagcttttgt ccttatatga
     6001 ttccttgtgc gtacggaaat ttacggtgtc tccgcagtat tcctgttttg caagaatact
     6061 gcggacaaca tatccgctcc aactgaacgg gtgggtaaac tcctttttgt ttttccttat
     6121 tccaattcct tttttgtaaa gtaaacagcc ggggtttcaa ccttgtcatc aaacagcgtg
     6181 cgggctatgt cataaggtcc gcagccttct attgtgaaat ggaaaattct gcggacgact
     6241 gtagccgctt cctcatcaat cagccaatga tttttgttgt tcgggtcttt cttgtaaccg
     6301 taaatagccg agttggttgt cggctttccc gattcgtgca gatagtttgc aatatcaaaa
     6361 gcatactatg cccgagcgta agtgtagcaa ctggtttgac ggttatgtta caaaaacaaa
     6421 tccacgtcta aaaggtaaca caagataaca aaaaactgta cagccgatta tggttgtaca
     6481 gtttttagtt ttatatgtta ttttatagga atgtaacatt gtacaaattt gcacaatttg
     6541 agccgtaggg gattgctaat cctacccgat aaagccttga acgaacacaa taacaatcag
     6601 cacggggagt gcgtatttaa ggtacggctt cagcactctt gaaagtttaa gacctttacc
     6661 cgtgttacat tcttcaaagt aattgtcaaa gccccagccg aattttgttg tgcagaacag
     6721 gagataaacc attgcgccaa tcggaagaag aaggttgctg acaacgaagt cctcaaggtc
     6781 aagcacaccc ttgccgagaa tgttgaagtc gctccaaatg ttgaatccga aaatacacgg
     6841 cagacacgca aagaacataa taattccgtt gattattgac gcctttgttc tgctcatgcc
     6901 gaatgtgtcg gtaagaaaag caacaaggtt ttcaaacacg gcaataaccg ttgaaaagct
     6961 tgcaaatgtc ataaacagaa agaaaagcgt accccaaatc cgaccgcccg tcatatttac
     7021 gaaaacattc ggaagcgtca caaaaataag ggcaggtcct tggtcgggct gaacgccaaa
     7081 tgagaagcaa gcagggaaga tgataagtcc ggcaacaatt gcaacaaaag tgtccaatgc
     7141 acaaatctga acagcctcac cgtaaagcgt gcggtctttg ttcatataac tgccgaaaat
     7201 ttccattgat gcgattccga gtccgagtgt gaaaaacgcc tgactcattg cagaggtgat
     7261 taccttgccg agtcctacat ctttaacctt tccgaaatcg ggaacaagat aaaagctcaa
     7321 gccttctccc gcatttgaaa gcatcatact gttgattgca agaataataa tcagcgcaag
     7381 caacgcaacc atcataacct tgctgacctt ttcaatgcct ttttgaagtc ctcgactgca
     7441 tacaaaaaat ccgaatacaa cgattatcgc aagccatata atcatctcaa caggcgatgc
     7501 cataaggtcg ttaaacactc ccgaaacctg ttctgagttc attccgcttt taaatgttcc
     7561 cgttgcaaat ttatagaagt aatctaacat ccaacccgaa acagg
//
LOCUS       DAOMVE010000089         7549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2569_length_7549_cov_3.467174, whole genome shotgun sequence.
ACCESSION   DAOMVE010000089 DAOMVE010000000
VERSION     DAOMVE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_7549_cov_3.467174"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..654)
                     /gene="rplJ"
                     /locus_tag="PLS64_06050"
     CDS             complement(94..654)
                     /gene="rplJ"
                     /locus_tag="PLS64_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRL43426.1"
                     /translation="MPSQSVLEQKKAIVAELSERLKSSITGVVVSYEGINTEDDTKLR
                     KELRENDVKYTVVKNTLLSRACEEAGLDDIKPVLEGTTAIATSDSEYAAAARILCNYA
                     KDHDNFKVKSAYLDGAVIDMDTIVALSKLPTREVLLANVLGAFQAPIASFARAVQAIV
                     DKGGVEACAAEAAEEAPAEAEAPAAE"
     misc_feature    complement(684..823)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(851..1546)
                     /gene="rplA"
                     /locus_tag="PLS64_06055"
     CDS             complement(851..1546)
                     /gene="rplA"
                     /locus_tag="PLS64_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRL43427.1"
                     /translation="MKHGKKYVDAAKLVDRTKLYDTNEALDTVIKTAVAKFDETVELH
                     VKLGVDSRHADQQVRGAVVLPNGTGKKVRVLAICKGANEELAKEAGADFVGAEDMTQK
                     IQQENWMDFDVLITTPDCMGLVGRLGRILGPRGLMPNPKAGTVTPDIAKAVKEAKAGK
                     IEYRLDKTNIIHCPIGKASFGAEKLGENLDTLMGAIVKAKPAAAKGQYIRSVAITSTM
                     GPSVRINPAKFGA"
     gene            complement(1603..2028)
                     /gene="rplK"
                     /locus_tag="PLS64_06060"
     CDS             complement(1603..2028)
                     /gene="rplK"
                     /locus_tag="PLS64_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRL43428.1"
                     /translation="MAQKVVGLIKLQIPAGKATPAPPVGPALGQHGVNIVAFTKDFNE
                     RTKNDIGLIIPVVITVYADRSFTFITKTPPAAVLLKKACGIDSGSGVPNKTKVAQITR
                     EQITKIAEQKMPDLNAGSLDAAISMISGTARSMGITVVD"
     gene            complement(2142..2669)
                     /gene="nusG"
                     /locus_tag="PLS64_06065"
     CDS             complement(2142..2669)
                     /gene="nusG"
                     /locus_tag="PLS64_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRL43429.1"
                     /translation="MAESEARWYVVHTYSGYENKVATDLMTTAENRNLQDMIIDVKVP
                     VEIVTETVGDKTKEVENKLFPGYVFVKMVYTDETWYVVRNIRGCTGFVGPGSKPVPLT
                     DAEVYRMGVETRSVKLNYSVGDSVRIIDGPLEDFVGVVEEIDADKDYVRVVVSMFGRE
                     TPVELELAQAEPIED"
     gene            complement(2714..2971)
                     /gene="secE"
                     /locus_tag="PLS64_06070"
     CDS             complement(2714..2971)
                     /gene="secE"
                     /locus_tag="PLS64_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRL43430.1"
                     /translation="MAEKKSENAKAAAKKTAKKPNVFKRLAKYLGSCKSEIKKITWPT
                     AKQTTKNFGIVIAMVAIVGVFIFALDQGLYALVGLFMNTTA"
     gene            complement(2984..3133)
                     /gene="rpmG"
                     /locus_tag="PLS64_06075"
     CDS             complement(2984..3133)
                     /gene="rpmG"
                     /locus_tag="PLS64_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRL43431.1"
                     /translation="MRVKITLACTECKQRNYNTMKNKKNSPDRLEMNKYCRFCKKHTL
                     HKETK"
     gene            complement(3344..4132)
                     /locus_tag="PLS64_06080"
     CDS             complement(3344..4132)
                     /locus_tag="PLS64_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43432.1"
                     /translation="MEFKKAFDYIVGKVEEELVKQNYTKQKVVAENDNELVSLFTSAS
                     LAYSVVYYKDKEQMVLRSCTMTDEGPDNEWKTLATWLYDETVGTQKDADSIANDFIEG
                     VSGTLAIKRAKQVKQKKKKDDDGTADPKFLAKRFVTYFPELREEIKNEEDCYFPFRGA
                     TFAKEHIAPKIPMYIKRANKNEIEKFANVFNVQYNNGDVDTRAIITIVLLNSLDDAEY
                     NALYEHFNDELKVAALNARAFKGKTVKPEKVKKVKAKANTLTKN"
     gene            4271..5089
                     /gene="murI"
                     /locus_tag="PLS64_06085"
     CDS             4271..5089
                     /gene="murI"
                     /locus_tag="PLS64_06085"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRL43433.1"
                     /translation="MSCQDAIGVFDSGLGGLSAVKEFLHVLPNEKIIYFGDTGRVPYG
                     NRSRETISKYALQDANFLLKHNVKTVVAACGTVSSVAGDMLEEKLSVPYTGVVNPTAF
                     IADRKTKNGKIGIIGTAATISSHSYKLRLEKLNPKYKVYEQACPLFVPLVENGFICRD
                     DQIVRLVIRRYLSELKETGVDTLILGCTHYPLLRDAIADFMGDGVTLVDSGYETAIYC
                     SKILRENNLLNTDGVHQTPEFYVSDTPDGFKSVAGLFLGRNMEHTVTQIDIEQY"
     gene            5101..5565
                     /locus_tag="PLS64_06090"
     CDS             5101..5565
                     /locus_tag="PLS64_06090"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HRL43434.1"
                     /translation="MTDKEKKKCKYMISILGEQILDDESDRIEVLTEGNFLMKKDHCY
                     IGYKEYDEDAPQSFYDNLIKVQDNTVTITRKGPMRSQLMLEKGRRHQCLYQTPAGDLM
                     IGVFTKTMNNSLTKNGGTLEVSYTLDFNNDLVSENKFKITVEEKEKFENEVD"
     gene            5567..7303
                     /gene="argS"
                     /locus_tag="PLS64_06095"
     CDS             5567..7303
                     /gene="argS"
                     /locus_tag="PLS64_06095"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRL43435.1"
                     /translation="MDTYALAVQQLKKAIENAIEKAITNGELPQAETAPFIIETPADR
                     SHGDFATNAAMAGAKAFRMPPFKIAQAITSNLDLEGTMFDRFETAGPGFINFFLGTEF
                     YSAVIREILANPEAYGRSEYGRDEKVMVEFVSANPTGPMHMGNARGGALGDCLAAVLD
                     KAGYNAWREFYVNDAGNQIEKFGCSLEARYLQIFKGDEIEFPEDGYQGEDIKDLAKEY
                     ADLNGDSLVNVSAEERKKALVDYALPKNIKKMQADMEKYKIFYDKWFFESELHKSGAV
                     KAVVDLLTKKGLTYEKDGAVWYKNKEVLTQKLLKEGKSQKYIDNLELKDDVLIRQNGN
                     PTYFTADIAYHKNKFDRGFTTLIDVWGADHHGHVMRMKGAMDAIGYDGDKLNVVLMQL
                     VKLVKDGELVRMSKRTGKAIQLGDLLDEVPVDSARFLFNTKEANTQMDFDLDLAVSQD
                     NQNPVYYVQYAHARICSIFKSLAKEGISPRECTDAELALLTAPEEKELINHLASYTNE
                     IISAAKDYDPTKVTRYVTQLATLFHKFYNACRVKGEEEGLMQARLALCNCVRAVIKNV
                     LTMFNISCPESM"
BASE COUNT         2120 a         1698 c         1584 g         2147 t
ORIGIN      
        1 ttaattcttc aataatagca gtaattttct ctgatgccat tgtaatttac ctccaatatg
       61 ataattagta aaattttaat taattgcagc cgattattct gctgcaggag cttctgcctc
      121 agcaggagcc tcttctgctg cctcagcagc acaagcttca acgccgccct tgtcaacgat
      181 agcctgtacg gcacgtgcaa aggatgcgat aggtgcctgg aatgcaccaa gaacatttgc
      241 aagaagaact tctcttgttg ggagctttga aagggcaacg attgtgtcca tatcaattac
      301 ggcaccgtca agataagctg acttaacctt aaagttgtca tgatccttag catagttgca
      361 aaggattctt gcagcagcag cgtactcgct gtcgcttgta gcaatagctg ttgtaccctc
      421 gagaacaggc ttaatgtcat caagacctgc ttcctcgcaa gcacgggaaa gaagtgtgtt
      481 ctttactaca gtgtacttaa catcgttctc acgaagctct tttctgagct ttgtatcgtc
      541 ctctgtgttg ataccctcgt agcttacaac cacacctgtg attgagcttt tgagtctttc
      601 tgaaagctct gcaacaattg ctttcttctg ctctaaaaca ctttgacttg gcaagataaa
      661 ttcacctcca aaatatttgt aacgcagttc ataaaaaaca gccgtccgtt ttacctcgga
      721 cggctgcaaa aacacaaaac aatgtaattg catcttcctc ggcaggcagg aaaaaccttt
      781 acgcttacgc acctgctgtc tttagaacag cgaatttaat atattcacac tattgtgtaa
      841 ataactgaaa ttaagcacca aacttagcag ggttgatgcg aacgctcgga cccattgttg
      901 aagtaatggc aactgagcgg atgtactgac ccttagctgc agcaggctta gcctttacga
      961 tagcgcccat gagagtgtca aggttttcac cgagcttctc agcgccaaag cttgccttac
     1021 cgattgggca gtgaatgatg tttgttttgt caagacggta ctcaatctta cctgccttag
     1081 cttctttaac agccttggca atgtcaggag ttacagtacc tgcctttggg tttggcataa
     1141 gaccgcgggg accgaggatt ctaccgagac gacctacaag acccatgcaa tcgggagttg
     1201 taataagaac atcaaagtcc atccagttct cctgctgaat cttctgtgtc atatcctcag
     1261 caccgacaaa gtcagcaccg gcttccttgg caagctcttc gttagcgccc ttacaaattg
     1321 caagaactct aaccttttta cctgtaccgt taggaagaac aaccgcacct ctaacctgct
     1381 ggtcagcatg acggctgtca acgccgagct ttacatgaag ctcaactgtt tcgtcaaact
     1441 tagcaactgc agtcttgata actgtgtcaa gtgcctcgtt tgtatcatat aatttagttc
     1501 tgtcaacgag cttagcagcg tcaacatatt tcttaccgtg tttcatcagt cttcctccat
     1561 tgttaggtgg tcaatcggaa ttatcctccc acccggaaat aattagtcaa ctactgtaat
     1621 acccatgctt cttgctgtac cggaaatcat gctgatagca gcatcaagtg aacctgcgtt
     1681 gaggtcaggc atcttctgct ctgcaatctt tgtgatctgc tcacgagtga tctgagctac
     1741 ctttgtctta ttaggtacgc ctgaaccgct gtcgataccg caagccttct taagaagaac
     1801 cgcagcaggc ggagtctttg taatgaaagt gaatgaacgg tctgcatata cagtaattac
     1861 taccggaatg ataagaccga tatcattctt tgtacgctca ttaaaatcct ttgtgaacgc
     1921 aacaatgttt acaccgtgct gaccgagagc cggaccaaca ggtggtgccg gagtagcctt
     1981 tccggcggga atctgcagct taattaagcc tacaaccttc tgagccattg tgtttgcacc
     2041 tccaaaattc gtggtaaatt gcgggacgcc actgtgtagg gtccctccca cagggagcgt
     2101 tacactccca ataaaaagcc aatcggctga ttattatttg attaatcttc tatcggttca
     2161 gcctgagcaa gctcaagttc aaccggagtt tctcgtccga acatagaaac aactacacgg
     2221 acataatctt tgtcagcatc aatttcttca acaacaccga caaaatcctc aagcggaccg
     2281 tctataatgc gaactgaatc gccgacagaa tagttaagct taacgcttcg tgtttcaaca
     2341 cccattctgt aaacttccgc atctgtaagt ggtacgggct ttgatccggg tcccacaaat
     2401 cctgtacagc ctctgatgtt gcgcacaaca taccaggttt cgtctgtata gaccatctta
     2461 acgaaaacat agcccggaaa cagtttgttt tcaacctctt tggtcttatc gccgactgtt
     2521 tcggttacga tttcaacagg aactttgaca tcaataatca tatcctgaag atttctgttt
     2581 tcggctgtgg tcataagatc tgttgcaacc ttattttcat aacccgaata ggtatgaaca
     2641 acataccatc ttgcttctga ttctgccata acaactttct tcctttcgtg attcaagcaa
     2701 tgtcagatac gaatcaggcg gttgtgttca taaaaagacc aacgagagcg tacaaaccct
     2761 gatcaagggc aaagataaat acgcctacaa tggctaccat agcaatgaca ataccaaagt
     2821 tctttgttgt ctgcttcgct gtgggccatg tgatcttttt gatttcactc ttacaggagc
     2881 cgaggtactt tgcgagcctt ttgaaaacat taggcttttt agcagtcttt ttagcagctg
     2941 cttttgcatt ttcagacttt ttctcagcca tgatgtacct ccgttatttt gtttccttgt
     3001 gaagagtgtg ttttttacag aaacggcagt acttgttcat ttcaagtctg tcaggactgt
     3061 tcttcttgtt cttcattgtg ttgtaattac gctgtttgca ctctgtgcag gccaatgtga
     3121 tttttactct cataacggca cctcccggtt ttttcggaat attttagcct ccctcaatag
     3181 ggcacaaaaa aataagcccc tttcagaggt aaggttattc taccatatat aaaggcttta
     3241 gtcaagcctt ttttgcaatt tatccgcaga aattctgtgt tttctgcgat attccgtaaa
     3301 acccgcccgt caagacggga ttttacggtg aatttcaatt aaatcagttc tttgtaagag
     3361 tgtttgcctt tgccttaact ttcttaactt tttcaggctt taccgtcttg cccttaaaag
     3421 cccttgcatt taaagcggca accttgagtt cgtcgttgaa atgctcataa agtgcattat
     3481 actcagcgtc atcaagtgaa ttgagcaaaa cgattgtgat gattgccctt gtgtcaacat
     3541 caccgttgtt gtactgaaca ttgaacacat ttgcaaactt ctcgatttca ttcttatttg
     3601 cacgcttaat atacatggga atcttcggag caatatgctc ttttgcgaat gttgcgcctc
     3661 tgaacgggaa gtaacaatct tcctcgtttt taatttcctc acgaagctcg gggaaatatg
     3721 ttacaaaacg ctttgcgaga aacttcggat cggcggttcc gtcgtcatcc tttttctttt
     3781 tctgcttaac ctgctttgcc cttttgattg caagtgtacc cgaaacgcct tcgataaagt
     3841 catttgcgat actgtcggcg tccttctgtg tgccgactgt ttcatcgtaa agccatgttg
     3901 caagtgtttt ccattcgttg tcgggacctt catcggtcat tgtgcaggaa cggagcacca
     3961 tctgttcctt atccttataa tatactaccg aatatgcaag tgacgcactt gtaaaaagcg
     4021 aaacaagctc gttgtcattt tcggcaacaa ccttctgctt tgtataattc tgttttacaa
     4081 gttcttcttc aaccttgccg acgatataat caaaagcttt tttaaattcc aacttaatct
     4141 tcctctcgga ttagtctgtc agaattcatt atacaatcaa ataacagatt tgtcaaaggt
     4201 tttgacttgc aagacgcaca ttattgtgtt attatttaag ctgtatatta atgtaaaaga
     4261 aggttttatt atgtcttgtc aagatgctat cggagttttt gactccggat taggcggact
     4321 cagcgctgta aaagagtttt tgcatgtgct gccaaacgag aaaataatat actttggaga
     4381 tacgggaaga gtaccctacg gcaacagaag tcgtgaaaca atttcaaagt atgcccttca
     4441 ggacgcaaat tttctgttga aacacaatgt taaaacagtt gttgcagcct gcggtacggt
     4501 aagttcggtt gcaggcgata tgcttgaaga aaagctttcc gttccgtaca cgggtgttgt
     4561 aaacccgacc gcattcattg ccgaccgaaa aacaaagaac ggtaaaatcg gaattatcgg
     4621 cactgccgca accatttcga gccacagcta caagctccgt cttgaaaagc ttaatccgaa
     4681 atacaaggtt tatgagcagg cgtgtccgct ttttgttccg cttgttgaaa acggtttcat
     4741 ttgtcgtgac gatcagattg tacggcttgt catcagaaga tatctttccg aactcaagga
     4801 aacaggcgtt gacaccctca ttctcggctg tactcactat ccgcttttgc gtgacgcaat
     4861 tgctgacttt atgggtgacg gcgttactct tgttgattcg ggatatgaaa ctgcaatcta
     4921 ttgctcaaaa attctcagag aaaacaatct gctcaacaca gacggtgtgc atcagactcc
     4981 cgaattttat gtaagcgaca ctcccgacgg atttaaaagc gttgcaggac tgtttctcgg
     5041 cagaaatatg gagcatactg ttacacaaat tgacattgaa cagtattaac gagggaagat
     5101 atgacagata aggaaaagaa aaaatgcaaa tatatgattt ccattctggg cgaacagatt
     5161 cttgacgatg aaagcgacag aattgaggtt ctcacagagg gaaattttct gatgaaaaaa
     5221 gaccattgtt acataggcta caaagaatat gacgaggatg caccgcagag cttttacgac
     5281 aatctcataa aagttcagga caatacggtg acaattacac gcaaaggtcc catgcgttca
     5341 cagcttatgc ttgaaaaagg cagacggcat caatgcctgt atcagacgcc tgcaggcgac
     5401 cttatgatag gagttttcac aaaaacaatg aacaactccc tcaccaaaaa cggcggaacg
     5461 cttgaggtca gttacacgct tgatttcaac aatgaccttg tgagcgaaaa caagtttaaa
     5521 attacggttg aagaaaaaga aaagtttgaa aatgaggtag attgatatgg atacatacgc
     5581 acttgctgta caacagctaa aaaaagcaat tgaaaacgct attgaaaagg caataacaaa
     5641 cggtgaactt ccgcaggcag agaccgctcc gtttatcatt gaaacacccg cagacagaag
     5701 ccacggcgac tttgcaacaa atgcggcaat ggcaggcgca aaggcattcc gtatgccgcc
     5761 tttcaaaatt gcacaggcta tcacatcaaa ccttgacctt gagggtacaa tgtttgaccg
     5821 ctttgaaaca gcaggtccgg gattcatcaa cttcttcctc ggcactgaat tttattcggc
     5881 tgtaatcaga gaaatccttg ccaatcccga ggcatacggc agaagtgaat acggcagaga
     5941 tgaaaaggtt atggttgagt ttgtatcagc caacccgaca ggtcctatgc acatgggcaa
     6001 cgcaagaggc ggcgctctcg gtgactgtct tgccgctgtt cttgacaaag cgggctacaa
     6061 cgcatggcgt gaattttatg taaacgacgc cggcaatcag attgaaaagt tcggctgttc
     6121 acttgaagca agatatcttc agattttcaa gggcgatgag atagagtttc ccgaggacgg
     6181 ctatcagggc gaggacatca aagaccttgc aaaagaatat gccgacctaa acggcgacag
     6241 ccttgttaat gtaagcgcag aagaacgcaa aaaggctctt gttgactatg cacttcctaa
     6301 gaacatcaag aagatgcagg ctgatatgga aaaatacaag attttctatg acaagtggtt
     6361 ctttgaaagc gagcttcaca agagcggtgc tgttaaggct gttgttgacc ttctcacgaa
     6421 aaaaggactt acctacgaaa aagatggtgc tgtttggtac aagaacaaag aggtgctcac
     6481 acagaagctt ctcaaagagg gcaagtcaca gaaatacatt gacaaccttg aattaaagga
     6541 cgatgttctc attcgtcaga acggcaaccc gacatatttt acagccgaca ttgcatacca
     6601 caagaacaaa tttgaccgtg gattcacaac tcttattgat gtttggggag cagaccatca
     6661 cggtcatgtt atgagaatga aaggcgcaat ggacgctatc ggctatgacg gtgacaagct
     6721 caatgttgtt cttatgcagc ttgttaagct tgttaaggac ggtgagcttg taagaatgag
     6781 caagcgtacc ggcaaggcta ttcagcttgg cgatttgctt gacgaagtgc ctgttgacag
     6841 cgcaaggttc ctctttaaca ccaaagaggc taacacacag atggactttg accttgacct
     6901 tgcagtaagt caggacaatc agaaccctgt ttattatgtt caatacgcac acgcaagaat
     6961 ttgcagtatc ttcaagtcac ttgcaaaaga gggcatttct ccgagagaat gtacagacgc
     7021 agagcttgca ctcctcacag ctcccgaaga aaaggaactc atcaatcatc ttgcgtcata
     7081 caccaacgaa atcatcagtg cggcaaagga ttacgatccg accaaggtta cacgctatgt
     7141 aacacagctt gcaacacttt tccacaaatt ctacaacgct tgccgtgtta agggcgaaga
     7201 agaaggtctt atgcaggcaa gacttgcact ttgcaactgc gtaagagccg ttatcaaaaa
     7261 tgttcttaca atgttcaaca tctcttgccc cgaaagcatg taaaccgagt acctcggtgc
     7321 gtagttcgtg tagatagttt tattaatgcg aagcttatat ttcccgagcg ttactattgt
     7381 atatctcgtg ctttattcaa ccgagtacct cggtgcgtag ttcgtgtaga tagttttatt
     7441 aatgcgaagc ttatatttcc cgagcgttac tattgtatat ctcgtgcttt attcaaccga
     7501 gtacctcggt gcgtagttcg tgtagatagt tttattaatg cgaagctta
//
LOCUS       DAOMVE010000090         7516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2582_length_7516_cov_3.370192, whole genome shotgun sequence.
ACCESSION   DAOMVE010000090 DAOMVE010000000
VERSION     DAOMVE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2582_length_7516_cov_3.370192"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLS64_06100"
     CDS             complement(<1..258)
                     /locus_tag="PLS64_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43436.1"
                     /translation="MGEKYYWVLGFCCGFIAVVIVTLIIANIKRKKNTYTEYDERQVL
                     ARGKAYKSAFFVLIGYIIACALVNVLEINWAELSVQMFIGLF"
     gene            complement(554..751)
                     /locus_tag="PLS64_06105"
     CDS             complement(554..751)
                     /locus_tag="PLS64_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43437.1"
                     /translation="MAENKNSRARIEANNRYNAKAYDRINVAVPKGRKDIIKAHAEKN
                     GESVNGFVNRAINETMQRDGE"
     gene            complement(1246..1479)
                     /locus_tag="PLS64_06110"
     CDS             complement(1246..1479)
                     /locus_tag="PLS64_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HRL43438.1"
                     /translation="MTCFYCKGDLKPSTTTHFVDLKKCIVIVKNVPCLECSKCGETVF
                     TDEVAKRLDDIVKTVSSLMTEIAVVEYTSDKVA"
     assembly_gap    1658..1667
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1985..2060)
                     /locus_tag="PLS64_06115"
     tRNA            complement(1985..2060)
                     /locus_tag="PLS64_06115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2025..2027),aa:Thr,seq:ggt)
     gene            2507..2986
                     /gene="rimP"
                     /locus_tag="PLS64_06120"
     CDS             2507..2986
                     /gene="rimP"
                     /locus_tag="PLS64_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HRL43439.1"
                     /translation="MAKSKGGVTVSKVWELCQPIVEGFGLSLWDVRYVKEGADRFLRV
                     YIDKPEGIDINDCENVSRAINGPLDELDPIEEAYCLEVCSPGIERELIRDEHFMQFIG
                     ADIMVKMLRPIDGIGREFKGVLKDYSDGKVTIEDHSGENEITISKKDAAWIKLDDFD"
     gene            3072..4232
                     /gene="nusA"
                     /locus_tag="PLS64_06125"
     CDS             3072..4232
                     /gene="nusA"
                     /locus_tag="PLS64_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRL43440.1"
                     /translation="MTNKDLFEALRTFEKEKGIPMDYMLQNIEKAISVACKNYYGGNE
                     NVVFKVDPEKNSFDAKLVKTVVDEVFDPNFEVTVEEAQQINKRKKFIVGDEIEVPIDP
                     KHLGWTSVSSARNVIRQGIRQGEKGQTLIEFQSKLGEIVTATVERIDPKSGVATIKIG
                     KSEATLPKSEQLGHDDLKEGDHIKVYIADVKDNERGPHAIISRTHPGLVKRLFEQEVP
                     EIYDGVVEIKSISREAGSRTKMAVYSSNPDVDAIGSCIGNKGARVNKIVEELGGEKID
                     IVLYSDDPEKYISAALSPASVCKVEITDEETKSCRATVPDGQLSLAIGNKGQNARLAA
                     RLTGWRIDIRPESGFYGEDEDDEPKTEEKSEDVLDLDESTEGETVVSDETEE"
     gene            4257..4565
                     /locus_tag="PLS64_06130"
     CDS             4257..4565
                     /locus_tag="PLS64_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRL43441.1"
                     /translation="MKQRKMPLRKCTGCGEMKEKRELIRVVKAPDTKDENGEIIAKGE
                     ISLDLTGRKAGRGAYVCRNLACFEKARKARRFERSLSCKIPDEVYEQMQSELAAADSK
                     "
     gene            4581..4913
                     /locus_tag="PLS64_06135"
     CDS             4581..4913
                     /locus_tag="PLS64_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HRL43442.1"
                     /translation="MNDRILSLLGICKRAGKLITGADTTIDSIRKSKAKLVIFANDFS
                     KSSKKSVQTAADECNVKTLTMNRSKEEIGFALGKICGVMTTEDKGFADKLEQLIENES
                     GGELYDKI"
     gene            4900..7263
                     /gene="infB"
                     /locus_tag="PLS64_06140"
     CDS             4900..7263
                     /gene="infB"
                     /locus_tag="PLS64_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRL43443.1"
                     /translation="MIKYKVKDAAADLGVSNKEIIEILEKHCGVTKKTMTTLEESELD
                     VIFDVITKKNKVENFDEYFAARNNKLDNESKPQQEDKPAKQNAKADNQKKKTDKNDKK
                     PNNKDAKPASKQEKVEVTQEKSVEESAPRKRRVIDTRATNVDVERYNQKYDDLASDSS
                     KGRSTDNIVKKQKFTNRTQRQKGRRQKRETEQERLNRIALERKQKPITVQIPDEIVVS
                     ELALRLKATVAEVVKKAFLMGTMVTATDTIDFDTASLIAMEFHAKVEKEVVVTIEEQI
                     IDDSEDDEANLVERAPVVVVMGHVDHGKTSILDAIRHANVTAGEAGGITQHIGAYRVM
                     VNGKPVTFLDTPGHEAFTTMRARGAQVTDIAILVVAADDGIMPQTVEAIHHAKAAGVS
                     VIVAINKMDKVGANPENVKQQLTEYELIPEEWGGDTPCIPVSAKTKEGLDDLLEMVTL
                     VAEMKELKANPDRAAKGTVIEARLDKGRGPIATVLVQNGTLHKGDTIIAGTCVGRVRV
                     MTNDKGERVTEAGPSVPVEITGLDEVPVGGDIFDAVSDERLARTLVDQRKAAKKEEIF
                     NAQTKVTLDNLFDQMKLGEIKELQIIVKADVQGSAEAVKQSLEKLSNDEVRVNVIHSA
                     VGAINESDVMLAEASNAIIVGFNVRPDAVAAENAERSGVDMRLYSIIYDCINEIESAM
                     KGMLAPKTREVVLGMAECRNVIKIKSVGTIAGSYVKSGKIQRGASVRVIRDGIVIADD
                     AIASLQRFKDAVKEVSEGYECGIGLERFNDLKEGDMFEVYTIEEYRD"
BASE COUNT         2399 a         1492 c         1662 g         1953 t
ORIGIN      
        1 aaacagtcct atgaacattt gaacgctgag ttcagcccaa ttaatctcca aaacattaac
       61 taaagcgcag gcaattatgt acccgataag cacaaaaaat gccgatttat aagccttgcc
      121 ccgtgcaaga acctgtcgtt catcgtattc tgtgtaagtg ttctttttcc ttttaatgtt
      181 tgcgataatg agcgttacga taactacggc gataaaaccg caacaaaaac caagcaccca
      241 atagtatttt tctcccataa taacatctcc caaataaatc agccaatttg cagatttatt
      301 cgtccaaccg ataatccgca aataatatta aaaaatttta tgtgtttaga tgtgaatact
      361 taaaaaatcc acaatgcgta caaaaagctt tctgtaccat tatagtacat taaaagttac
      421 ttattgtcaa gtatatttta cataatttca aaaatacttt catttggctg ttttgaaaaa
      481 ttaaatccgt taagaaacct gtcaactaaa acaaaacaag acaagcccat atttcagagc
      541 ttgccttgtg gaattactca ccgtcacgct gcattgtttc attaatagca cgattaacaa
      601 aaccgttcac gctttcgccg ttcttttctg cgtgggcttt gataatatcc tttcgacctt
      661 tcggcacagc aacattgata cggtcatatg ctttagcgtt atatcggttg tttgcctcaa
      721 ttctcgccct gctgttctta ttttctgcca tttcttcacc gcctaaaatc tattgacatc
      781 aaattattgt tgttgtataa tataggtgaa aaggaacggc tttagctgtt tcgcatttgc
      841 gtattttttt attaaaaacc gctctgtatt gcgactacag ggcggttatt tctttatatt
      901 cttaatgact tcattaaaag ccacgaaaac aattgcgaga acaaacaagt atgtaatctc
      961 tgccattgga atacaccccc tttcgcaagg gagtcgaaac agccgccacc gttcctttac
     1021 aaatattatt atagcgcacc tctttacact taacaagtac caatatgcac aaattatacg
     1081 cttaaacatt gtagtataat atagtcacag gataaggaaa ggggaaagcg ataatcagta
     1141 tgaccgcaac gcaattgcag tatatgcaag cgtaggcagc ggcatagcag aaccccacag
     1201 gcaaaagaaa agacaagccc atatttcaga gcttgccttg tggaattatg ctactttgtc
     1261 agatgtatat tcaacaaccg ctatttcggt cataagactg cttacggttt ttacaatgtc
     1321 atcaagtctt tttgccactt catcggtaaa aacggtttca ccgcattttg aacattccaa
     1381 acacggaaca ttctttacga ttacaatgca ctttttaaga tcaacaaaat gtgttgttgt
     1441 acttggcttc aaatcacctt tgcaataaaa acaagtcatt gttatttctc ctttcgggtt
     1501 tgtgaatcac tttcaaattt atctgaatcg ggaaaatagg cagttactat acaaacaaca
     1561 tttccgttgc aacctgccac aatatgcaaa tattcattgt ttgtacttac acctaaaaca
     1621 aggcaagcgg gatatggata gctgtcggga tattgttnnn nnnnnnntga atacatcatc
     1681 acggtatatt cctcgttcct gtaaccgttt cagagcgtgc acagaccata tgacagattt
     1741 atcgtcacat aacttttgta atgcttcgat atttaattca ggcaagcttg tcacctcctc
     1801 tgtctatggt gtgtttgatt tgtgtattta ttcgcaacta attcgcaacc attctttaca
     1861 actttattgt agcatatcaa gatagattgt acaagaataa tattaatcag tttggatttg
     1921 gtaacggtat tgttttctaa acaacaataa aaaggtatcg gtctttttaa ccaataccct
     1981 tttatggagc tgataacggg attcgaaccc gtgacctcgt ccttaccaag gacgcactct
     2041 gcctactgag ctatatcagc atatttaatt gtaaggttgc aacctttata atgttaatct
     2101 cgcaaagttt cggacattaa cagccaaatc attgatataa aaaacagtaa aaatccaaca
     2161 ataacaaaat caaaattaat tttacttttt ttacacggtc gcaaaaccac ggtgattata
     2221 tcacaaaaac atcgtgatgt aaagtgtcca cggaatataa taacacattt gcacggtgat
     2281 ttcaaatgtt tttttaaact tttttgtaaa aaatacgaaa agatttgaaa aacacttgat
     2341 tataagccgt tttggtgcta taattttctt gtaaataatg aaagctaaag gagtgggcaa
     2401 caaacccgct ccttatttat tagttttcac tttgttcctc ggtgcggttt atctgtctgc
     2461 aatatttcgc acagggtatt tttgcagaca ggaaggcagt gaatttatgg caaaaagcaa
     2521 aggcggtgta accgtatcaa aagtatggga gctttgtcag ccgattgttg aaggcttcgg
     2581 actttcactt tgggatgtgc gttatgtcaa ggaaggtgca gaccgtttcc tgcgtgttta
     2641 cattgacaag cccgaaggca ttgacattaa cgattgcgaa aatgtttccc gtgcaatcaa
     2701 cggtcctctt gatgagcttg atccgattga ggaggcgtac tgtcttgagg tctgctcccc
     2761 cggaattgag cgtgagctta tccgtgacga acattttatg cagttcatcg gtgcggatat
     2821 tatggttaag atgctccgcc ccattgacgg cataggcaga gaatttaaag gggttttaaa
     2881 ggattattcc gacggtaagg tcacaattga ggatcacagc ggtgagaatg aaatcacaat
     2941 cagcaaaaag gacgccgcat ggattaagct tgacgatttt gattaaaaat tgcagtttga
     3001 acactacgga tacacataac agcataacaa tttgtatata accgaaaaat aagaaaagga
     3061 tgatttggaa aatgactaac aaagatttat ttgaggcatt gagaacattt gaaaaggaaa
     3121 agggcattcc tatggactat atgcttcaaa acattgaaaa ggcaatttcc gttgcctgca
     3181 agaactacta cggcggtaat gaaaatgttg tattcaaggt tgatccggaa aagaacagct
     3241 ttgacgcaaa gcttgtcaaa acagtagttg acgaggtttt tgatcctaac tttgaggtta
     3301 ctgttgagga ggctcagcag attaacaaga gaaagaaatt tattgtcggc gatgagattg
     3361 aagttccgat tgatcccaag cacctcggtt ggacatctgt ttcttctgca agaaatgtta
     3421 tccgtcaggg cattcgtcag ggtgaaaaag gtcagactct tatcgagttt cagagcaagc
     3481 tcggtgaaat tgttactgca accgttgaaa gaatcgatcc taagtcgggc gttgcaacaa
     3541 tcaaaatcgg caagagcgaa gctactctgc caaagagcga acagctcggt catgacgacc
     3601 ttaaagaagg cgaccacatt aaggtttata ttgcagatgt caaagacaac gaaagaggtc
     3661 ctcacgcaat catttcaaga acacaccccg gtcttgtaaa gcgtttgttt gaacaggaag
     3721 ttcctgaaat ctatgacggc gttgttgaaa taaagtcaat ctcccgtgag gcaggcagcc
     3781 gtacaaaaat ggctgtttac agctcaaatc ccgatgttga cgcaatcggt tcatgtatcg
     3841 gtaacaaggg cgctcgtgta aacaagattg ttgaagaact cggcggtgaa aaaatcgaca
     3901 tcgtgcttta cagcgatgat cccgaaaagt acatttcggc ggctctctct cctgcaagcg
     3961 tttgcaaggt tgaaattaca gatgaagaaa caaagagctg ccgtgcaacc gttcccgacg
     4021 gtcagctttc acttgctatc ggcaacaaag gacagaatgc ccgtcttgcc gcaagactta
     4081 caggctggag aattgacatc cgtcccgaaa gcggtttcta cggcgaagat gaggacgatg
     4141 aaccaaagac agaagaaaag tcagaagatg ttctcgacct tgacgaaagc acagaaggcg
     4201 aaacagttgt ttcggatgaa acagaggaat aatcagatta aacagagagg gattttatga
     4261 agcaaagaaa aatgccgttg cgtaaatgta cgggttgcgg agagatgaaa gaaaagcgtg
     4321 agcttatccg tgttgtcaaa gcacccgaca ccaaagatga aaacggtgaa atcattgcaa
     4381 aaggcgaaat ttcgcttgac ctcacaggtc gcaaggcagg cagaggcgca tatgtgtgca
     4441 gaaatcttgc ctgctttgaa aaggctcgca aggcacgccg ctttgaacgc tcgttaagct
     4501 gtaaaatccc tgatgaagta tatgagcaga tgcaaagtga acttgccgca gcagacagca
     4561 agtaggcgaa aggatttttt atgaacgaca ggattttgtc gctgcttggc atttgcaaga
     4621 gggcaggcaa gctgattaca ggtgccgaca cgacgattga ctcaatacga aaaagcaagg
     4681 caaagcttgt tatttttgca aatgattttt caaagagcag taagaaaagc gtgcagacag
     4741 ccgctgatga atgcaatgtg aaaacattga caatgaaccg aagcaaagag gaaatcggat
     4801 ttgctctcgg aaaaatctgc ggagttatga caaccgaaga caaaggcttt gcagataagc
     4861 ttgaacagct tattgaaaac gaatcgggag gagaattgta tgataaaata taaggtaaag
     4921 gacgcagcag cagatctcgg agtttcaaac aaggaaatta ttgagattct cgaaaaacat
     4981 tgcggtgtta caaagaaaac aatgacaaca ctcgaggaaa gcgaactcga tgtgattttt
     5041 gatgtaatca caaagaagaa caaggttgaa aactttgacg aatactttgc ggcacgcaac
     5101 aacaagcttg acaacgaaag caagccacag caggaagata agcccgcaaa gcagaatgca
     5161 aaggctgaca atcagaagaa aaagaccgat aaaaacgaca aaaagcctaa caacaaggat
     5221 gcaaagcctg catcaaaaca ggaaaaggtt gaagttactc aggaaaagag cgttgaggag
     5281 tctgctcccc gcaagcgcag agtaattgac acccgtgcaa caaatgttga tgttgagcgt
     5341 tacaaccaga agtatgatga ccttgcaagc gacagctcaa agggcagaag cacggacaac
     5401 attgttaaaa agcagaaatt cacaaaccgt acccagcgtc aaaagggcag acgacagaag
     5461 cgtgaaacag agcaggagcg tctgaaccgt attgctcttg agcgtaagca gaaaccgatt
     5521 acagttcaga ttcctgacga aatcgtagtt tcggagcttg ctctcagact caaggctacc
     5581 gttgccgagg ttgttaaaaa ggcattcctt atgggtacaa tggtaacggc aacagataca
     5641 atcgactttg acacagcgtc acttatcgcc atggaatttc atgcaaaggt tgaaaaggaa
     5701 gttgttgtta caatcgaaga acagattatt gatgacagcg aggacgatga ggcaaatctc
     5761 gttgagagag ctccggttgt tgttgttatg ggtcatgttg accacggtaa aacttcaatc
     5821 ctcgacgcta tccgtcacgc aaatgtaaca gcaggcgagg ccggcggtat cactcagcat
     5881 atcggtgctt acagagttat ggtaaacggc aagcctgtta cattcctcga cacacccgga
     5941 catgaggcat tcacaacaat gcgtgcaaga ggtgctcagg ttaccgatat tgcaatcctc
     6001 gttgtagccg ccgatgacgg tattatgccg cagacagttg aggctatcca ccatgcaaaa
     6061 gccgcaggtg tatctgttat tgttgccatc aacaaaatgg ataaggtcgg cgcaaatccc
     6121 gaaaatgtta aacagcagct cacagagtat gagcttattc cggaggagtg gggcggcgat
     6181 acaccgtgta tccctgtttc cgcaaagaca aaggaaggtc ttgacgatct tcttgaaatg
     6241 gttactcttg ttgcagagat gaaagaactc aaggctaacc ccgacagagc cgcaaagggt
     6301 actgttatcg aggcaagact tgacaagggc agaggtccta tcgcaaccgt actcgttcag
     6361 aacggtacac ttcacaaggg cgatacaatc atcgcaggca cttgcgtcgg tcgtgtaaga
     6421 gttatgacaa acgataaggg cgaaagagtt accgaggcag gtccttctgt tcctgttgaa
     6481 atcacaggtc ttgacgaagt tccggttggc ggcgatatct ttgacgcagt ttccgatgag
     6541 cgtcttgcaa gaactcttgt tgaccagaga aaggccgcta agaaggaaga aatcttcaac
     6601 gcacagacta aggttactct tgacaatctc tttgaccaga tgaaactcgg tgaaatcaag
     6661 gaacttcaga ttatcgttaa agccgatgta cagggttctg ccgaggctgt taagcagtca
     6721 cttgaaaagc tttcaaatga cgaggtaaga gtaaatgtaa ttcacagcgc agtaggtgca
     6781 atcaacgagt ccgatgtaat gcttgcagag gcatctaacg caattatcgt aggattcaac
     6841 gttcgtcccg atgcagttgc cgcagaaaat gcagagcgtt cgggcgttga tatgcgtctt
     6901 tacagcatta tctacgattg tatcaacgaa atcgaatctg ctatgaaggg tatgcttgct
     6961 cctaagacaa gagaagttgt tctcggtatg gctgagtgcc gtaatgttat caagattaag
     7021 tcagtcggca caatcgcagg ttcatatgtt aagagcggta agattcagcg tggcgcaagc
     7081 gtaagagtta tccgtgacgg tatcgttatt gcagatgacg caattgcatc tctccagaga
     7141 ttcaaggacg ctgtcaagga agttagcgaa ggctacgaat gcggtatcgg tcttgaaaga
     7201 ttcaacgacc tcaaagaggg cgatatgttc gaggtttata caatcgaaga atacagagat
     7261 taaagtttgt atgttaaacc aaattttata aacggcacaa attcaccgtt attttgtagg
     7321 ggcggtcatt gggcgcccgc aggtaacggc agaaattttc agtttaaata aatactgtat
     7381 agttcaagtc agtagctgta aaataacaaa atatttcatc aaagcgggcg gccgatgacc
     7441 gcccctacgg aaataagata aaaggatgtg atagcatggg aagccataga attgaaagaa
     7501 caacagagga catcaa
//
LOCUS       DAOMVE010000091         7470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2601_length_7470_cov_3.955496, whole genome shotgun sequence.
ACCESSION   DAOMVE010000091 DAOMVE010000000
VERSION     DAOMVE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_7470_cov_3.955496"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="PLS64_06145"
     CDS             complement(<1..1651)
                     /locus_tag="PLS64_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HRL43444.1"
                     /translation="MNKKFVEKFTAVVSAVCMISVGIASVNAAEIDDGFESVTKNAQS
                     SDSSFPSAYSAIGYSLVDENGLPSNFSSKNLGFVTPIKAQQYNDCWAVAGTEGFETKL
                     LKLGYPVTEMSHDHANASSTTQTNGKGWQRKYRDGGFTNIYPGYLTSWQGGAEIADVG
                     KIDFSKNIHGDDMTADKTKYGTTKLRYLDGADSNEIKQAIIDNGSVTASYATSNKCFN
                     NAGTTYFMPQSYDGDYVGHTISIVGWNDNLSRYRFSNGTGVLPQNNGAWLVRNSWGDN
                     NTMGGYFWLSYEDKYIFGEKYSPNFTIDEVTEITDDMTLLQDERYGATYSFNYVDSND
                     ITFINCFDFGENSRTLDKVLFETKSNGADYEIYYIPVRDGVPSNDESEWKSVASGKVA
                     YSGYQSVDANGFVAPLGRGAVGVRIKTNSEESSQLGVGEWLTSATKMTFLNDSSYGNS
                     YIKYDGTTCELLDWYKTERDDMLGGTFVIKAVALKNDKILNGDVDLDGDIAVKDATLV
                     QKYIVKLEQLDNIQLCNADCDGDGDITVADATKIQKIVVGIN"
     assembly_gap    1745..1844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1943..2818
                     /gene="gpr"
                     /locus_tag="PLS64_06150"
     CDS             1943..2818
                     /gene="gpr"
                     /locus_tag="PLS64_06150"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR01441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HRL43445.1"
                     /translation="MQFRTDLAIEAREIAGENTGGIEFKKYSENGLEISRLIVKNQKA
                     RQALGKEIGTYITIELPSLTDNFTETDKRLETVGNEIKRLLPVNGLVLVAGLGNMEIT
                     PDSLGPKTSRRVLATRHIGGEIARSTGLDRLRPVAVMQTGVTGQTGIETGEYILSIVR
                     RIRPTAVVAIDALASRRTERLGCTLQISDTGISPGAGVGNHRTKITKETIGVPVIAIG
                     VPTVVDAQTLAVDILGNDCNRKTQKMLMPQGRQLVVIPREIDLLTERASRLIAFALNG
                     ALQNEFDLPDLISLM"
     gene            2912..4009
                     /locus_tag="PLS64_06155"
     CDS             2912..4009
                     /locus_tag="PLS64_06155"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HRL43446.1"
                     /translation="MKSEGTSKKKLKKALKTSTAVVLLTTAVLSVIYRLPLCFESQNP
                     AALAAAAFTLTNGEYKLENSKTEKPTASDQKPTESADTEPTVQKNHKRDKSTYYDSYG
                     NHDGENKYTVEERTIGDTGTQVDNCFVKNKTGLSLDFDGLLSAKLPFSIDKGINSPQV
                     LIYHTHTSEAYLDEDVDFFYDSFYSRTNNNDFNVVAVGDALTEQLNKRGIKTVHDTTI
                     HDESYNGSYDRSVDTVYKNLEKYPDIKVVIDLHRDAIGTDENKVKPVFTYNGKKGAQI
                     MILAGCDTDGERGFDCWEENLNFALKIQDKAETLYPDMTRPLNFDYFAYNEYVCNGSL
                     LIEVGTESNSIDEATYSGSLLGNAIADVLLN"
     gene            complement(4188..5633)
                     /locus_tag="PLS64_06160"
     CDS             complement(4188..5633)
                     /locus_tag="PLS64_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HRL43447.1"
                     /translation="MNKRLIVYILGWVLIVEGAAMQIATVTSFIYGEHEGLYFLGVGA
                     LSALLGLLAVKVKKPKNPVLYQKAGFAATALSWILMSFVGCFPFWLSGEIPSFIDAFY
                     ETVSGFTTTGSTILTDVEALSHGMLMWRSFLHWLGGMGVIVFLLAIIPKLGGQQNIFL
                     MKAESPGPIVGKAVPKMRNYAMMLYGIYFGLTALEFIFLIVGKMPVFDALNISFATAG
                     TGGFGVTNAGIASYSNYLQTVIAVFMMLFGINFSVYILILAKKFKQAFRIQELWVYFG
                     IIVLSTALIAFNIRSLYPSAYDAVHQSFFYVSSIITTTGFGLTDVNNWPEFSKTVIMI
                     ITFIGAMAGSTGGGFKVSRVILLFKETRKEFSLLIHPRNVKTVKMDGKAIDHNVMRST
                     SIFLVLYIGIFALSWLVLSLDQTDFVTNFTAVAANINNTGPGLGMVGPVGNYSEFSIL
                     SKIILIFDMLAGRLELFPLMLLFAPSAWKKS"
     gene            complement(5644..6999)
                     /gene="trkA"
                     /locus_tag="PLS64_06165"
     CDS             complement(5644..6999)
                     /gene="trkA"
                     /locus_tag="PLS64_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HRL43448.1"
                     /translation="MRIIIVGCGKVGSSIASELNLEGHEISVVDINHGAVDKLANSLD
                     VLGIEGNGATYDVLIEAGAEYADLLIAATARDEINLYSCLMAKAAGVTHTIARVRNPE
                     YTNDLYRIKEHLGLSMAINPEQTAANEISRLLRFSGALEIDSFSKGVVELIKVTVPQN
                     SSIVGTALSRVDNLRGKVRICTVERGDEMFIPNGDFVINGGDRISVVAKPEIAAKFFK
                     RISIAIGRSKDVILLGGGKVSFYLAKTLIKSGANVKIIEKNPERCNFLSEAIPGAVII
                     QGDCMDQSLLLSEGVEHADGIAALMDYDEENIIITMYLKGITKAKLITKVNNASFDPL
                     LHDQNFECIIHPKSLTGEYIARYIRAMQNTGDSNVESLYRLNDDKVEALEFKVNAESR
                     VTNVPIMSLNLKANLQIICINRGGKIILPQGTDVIKPEDTVIVLTMQKGLSKLEDIVK
                     R"
BASE COUNT         2223 a         1751 c         1477 g         1919 t
ORIGIN      
        1 aattaattcc cacaactatt ttctgaattt tcgtggcatc ggcaaccgtg atatctccgt
       61 caccgtcaca atcggcattg caaagctgta tgttgtcgag ctgttcaagc tttactatat
      121 acttctgaac aagggttgcg tccttaacgg caatatctcc gtcaaggtca acatcgccgt
      181 tcaaaatctt atcatttttg agcgccacag ctttgataac aaatgtaccg ccgagcatat
      241 catctctctc ggttttgtac cagtcgagca gttcacatgt tgtgccgtca tatttaatgt
      301 agctgttacc gtaagatgaa tcattgagaa aagtcatttt cgttgcactt gtaagccatt
      361 cgcctacacc aagctgactt gattcctctg aatttgtttt gattctcaca ccgactgcac
      421 ctcttccgag cggtgcaaca aatccgtttg catcaacaga ctgataaccg ctgtatgcaa
      481 ccttgcccga tgcaacagac ttccattcgc tctcatcatt tgagggaaca ccgtctctta
      541 cgggaatgta gtaaatctca taatccgcac cgttgctctt tgtttcaaaa agcaccttat
      601 caagtgtacg gctgttttcg ccaaaatcaa agcaattgat gaatgtaatg tcgttgctgt
      661 caacataatt aaagctgtat gtagcaccgt agcgttcatc ctgcaacaat gtcatatcgt
      721 cggtaatctc cgtcacctcg tcaattgtga aattcgggga atacttttcg ccgaaaatgt
      781 acttatcctc atatgaaagc cagaaataac cgcccattgt gttgttgtcg ccccagctgt
      841 tgcgcacaag ccatgctccg ttgttctgcg gcaacacacc tgttccgtta gagaatctgt
      901 acctgctgag attatcgttc caacccacaa tgctgattgt gtggccgaca tagtcgccgt
      961 catatgactg cggcataaag taggttgtgc ctgcattgtt aaagcacttg tttgatgtgg
     1021 catatgacgc tgtcaccgag ccgttgtcga taattgcctg cttgatttcg tttgaatcag
     1081 cgccgtcaag ataacgcagt tttgttgtgc cgtatttagt cttgtcggct gtcatatcat
     1141 cgccgtgaat attttttgaa aaatcaatct taccgacatc ggcaatctct gcaccgccct
     1201 gccatgaggt gagatagccg ggataaatat ttgtaaatcc gccgtcacgg tattttctct
     1261 gccagccctt gccgtttgtc tgtgttgtgg aacttgcgtt tgcgtggtca tgtgacatct
     1321 cggtaacggg atagccaagc ttcaaaagct ttgtttcaaa gccttctgtg cctgcaaccg
     1381 cccaacaatc gttatactgc tgagccttga taggtgttac aaagccgaga ttttttgagc
     1441 tgaaattact cggaagtccg ttttcgtcaa caagcgaata gccgattgcg gaatatgcag
     1501 acggaaaaga cgaatccgaa gattgtgcat tctttgtgac cgactcaaat ccgtcatcaa
     1561 tttcagccgc atttacgctt gcaatgccga ccgaaatcat acacaccgca gaaaccactg
     1621 cggtaaattt ttcaacaaat tttttgttca taaaatcacc tgtatataat gtaaagatgt
     1681 gtgtttgttt tagaattata atctatctct ttttctttat gataaaggat ttaaatctga
     1741 acaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnattata acaaaacaaa
     1861 atagtcttgt aaatagaata cataatataa aattgcacaa taataacaat atacaaacaa
     1921 atttgcgaaa cgggggatta ttttgcagtt cagaaccgac cttgcaattg aggcaagaga
     1981 aattgcagga gaaaacacag gcggtattga attcaaaaaa tacagtgaaa acggtcttga
     2041 aatttcacgg ctgattgtaa agaaccaaaa ggcaagacag gcactcggca aggaaatcgg
     2101 cacatacatt acaattgagt tgccgtcact gaccgacaat tttaccgaaa ccgacaaacg
     2161 gcttgaaacc gtcggcaatg aaatcaaacg gctgttgccc gtaaacggac ttgtgcttgt
     2221 ggcagggctc ggcaacatgg aaatcactcc cgactcactc ggaccgaaaa caagccgacg
     2281 agtgcttgcc acccgtcaca taggcggaga aattgcccgt tcaacgggac ttgaccgact
     2341 tcgccccgtt gccgtaatgc agacgggagt cacgggacag acgggcattg aaacaggcga
     2401 atacattctc agcattgtac ggcggataag accgacagcc gttgttgcaa ttgacgcact
     2461 cgcctcacgc agaaccgaaa ggctcggctg tacacttcag ataagcgaca cgggaatctc
     2521 acccggtgcg ggggtgggca atcaccgaac caaaatcaca aaggaaacaa tcggagttcc
     2581 tgttattgca atcggagtgc cgaccgttgt tgacgcacaa acgcttgcgg ttgacattct
     2641 cggcaatgac tgcaaccgaa aaacgcagaa aatgcttatg ccgcagggca gacagcttgt
     2701 agtcattccg agagaaattg acctgctcac ggaaagagcc tcaaggctca tagcctttgc
     2761 actcaacgga gcgttgcaaa atgaatttga tttgcccgac ctcatctcgt taatgtaaat
     2821 ccacgcatat ttacacatat tcacctacgg cacggcatat atttattgca gaaaccgata
     2881 gccaacatat gtacggattg caggtgattt tgtgaaaagt gaaggaacat caaaaaagaa
     2941 actgaaaaaa gccttaaaaa caagcacagc cgttgtactg ttgacaacgg ctgttttaag
     3001 cgttatatac agactgcctc tctgctttga atcacagaat cctgcggcac ttgccgctgc
     3061 cgcttttact ctcacaaacg gagaatacaa gcttgaaaat tcaaagacgg aaaagccgac
     3121 agcttccgac caaaagccca cagaatcagc cgacacagaa cccacagtcc aaaaaaatca
     3181 caagcgtgac aaaagcacct attacgattc ctacggcaac catgacggcg agaacaaata
     3241 cacggttgag gaacgcacca taggcgacac gggaactcag gttgacaact gctttgtcaa
     3301 aaacaaaacg ggactgtcac ttgactttga cggacttctt tcggcaaaac tgccgttttc
     3361 aattgacaag ggcataaact caccgcaggt tcttatctac cacacccaca caagcgaggc
     3421 ataccttgac gaagatgttg actttttcta tgacagcttt tattcacgca caaacaacaa
     3481 cgatttcaat gttgtggcag tcggagatgc actcaccgaa cagctcaaca agcgtggaat
     3541 aaaaacggtt catgacacga caattcacga tgaatcctac aacggttcgt atgaccgtag
     3601 tgttgacaca gtgtataaaa atcttgaaaa atatcccgat atcaaggttg ttatcgacct
     3661 gcaccgtgat gcaatcggaa cggacgaaaa caaggttaag cccgtgttta catacaacgg
     3721 caaaaagggt gcgcagatta tgattcttgc gggttgcgac acagacggtg aacggggttt
     3781 tgactgctgg gaggaaaatt taaactttgc cctgaaaatt caggacaaag ccgaaaccct
     3841 ctatcccgat atgaccagac cgctgaattt tgactacttt gcatacaatg aatatgtctg
     3901 caacggttcg ctgttaatag aagtcggcac agaatcaaat tccattgacg aggcaaccta
     3961 ctcaggcagt ctgttaggca acgcaattgc agatgtgctt ttaaattgac ggcaagccgt
     4021 ctttcccagt tcgtgaagac agttcgtaaa tatcaaaaaa catattatgc ccgagcgtag
     4081 tattgtaatt attcgcacaa ttccacatat aaaaaacagt cgggaagaca ttgttttgca
     4141 ttttgcaaac tgtcttcccg atctttatta taatcttttc aatttactta cgattttttc
     4201 cacgctgacg gggcgaacag caacatcaac gggaacaatt cgagtctgcc tgctaacata
     4261 tcgaaaatca aaataatttt tgacaaaata ctgaattccg aatagttgcc gacagggcca
     4321 accattccaa gacccggacc tgtgttgttg atatttgcgg caacagccgt gaaattcgtc
     4381 acaaaatccg tctgatcaag cgaaagcaca agccagctta acgcaaagat tccgatgtac
     4441 agcacaagga aaattgaagt tgacctcata acgttgtggt caatcgcctt gccgtccatt
     4501 ttaacggtct taacatttct cggatgaatg aggagtgaaa actcctttcg tgtttcctta
     4561 aagagcaaaa taactcttga aaccttaaaa ccgccgcctg tactgcctgc cattgcaccg
     4621 ataaatgtga taatcataat cacggttttt gaaaattcag gccagttatt gacatccgtc
     4681 aaaccaaaac ctgttgtcgt gataattgac gaaacataga aaaagctctg gtgaaccgca
     4741 tcatacgcac tcggataaag tgacctgatg ttaaacgcaa tcaaggctgt tgacagcacg
     4801 ataattccga aataaaccca cagctcctga atgcggaaag cctgcttaaa cttcttcgca
     4861 agaatgagga tatacacgga aaagttaatt ccgaacagca tcataaatac agcaataacg
     4921 gtttgcagat aattgctgta tgaagcgata cctgcgttgg taacaccgaa accgcccgta
     4981 cctgccgttg caaagcttat gttaagtgcg tcaaaaacag gcattttgcc tacaatcaga
     5041 aatataaatt caagtgcagt caagccgaaa taaattccgt agagcatcat tgcgtaattt
     5101 ctcattttcg gaacagcctt gcccacaatc ggaccggggc tctcggcttt cataaggaaa
     5161 atattctgct gaccgccgag cttcgggata atcgcaagca agaacacgat tacgcccata
     5221 ccgcccaacc aatggagaaa gcttcgccac ataagcattc cgtggctcaa tgcctcaaca
     5281 tcggtaagta tagttgaacc tgttgttgta aagcccgaca cggtttcgta aaaagcgtca
     5341 ataaaactcg gaatctcacc gctcagccag aacggaaaac atcctacaaa agacataaga
     5401 atccagctta acgcagtagc cgcaaagcct gccttctgat aaagcacggg atttttcggc
     5461 ttttttactt ttacggcaag caagcccaaa agtgccgaca aagcacctac tccaagaaaa
     5521 taaagtccct cgtgttcgcc gtatataaat gaagtaacgg ttgcaatctg catagccgca
     5581 ccctcgacaa taagaaccca gccgaggata tacacaatta atcgtttatt cattatgctc
     5641 ccctcaacgc tttacaatat cttcaagctt gctaagacct ttttgcatgg taagcacaat
     5701 taccgtgtcc tcaggtttaa tcacatccgt accttgcgga agaataattt ttccgcctct
     5761 gttaatgcag ataatctgaa gattagcctt cagattaagg ctcataatcg gaacattcgt
     5821 aacccttgat tccgcattaa ccttgaattc aagagcctca accttgtcgt cgttaaggcg
     5881 gtaaagcgat tcaacattgc tgtcgcccgt attctgcatt gctctgatat atcttgcaat
     5941 atattcgcct gtaaggctct ttggatgaat aatacattca aaattctgat catgcaaaag
     6001 cggatcaaat gacgcattat ttaccttagt aatcaatttt gcctttgtaa tgccctttag
     6061 gtacatcgtt atgataatat tttcttcgtc ataatccata agagcggcaa tgccgtcagc
     6121 gtgttcaaca ccctccgaaa gcaacagcga ctgatccatg caatcgccct gaattataac
     6181 agccccaggt atagcctccg aaagaaagtt gcagcgttcg ggatttttct cgataatttt
     6241 gacattcgca ccggatttaa taagcgtctt tgcaaggtag aacgaaacct tgccgccgcc
     6301 gagaaggata acatccttac ttctgccgat tgcaatactg atacgcttaa agaattttgc
     6361 ggcaatttcg ggctttgcaa caacagaaat tctgtcaccg ccgttaataa caaaatcacc
     6421 gttaggaatg aacatctcat cccctctctc aaccgtacag attctcacct tacctctgag
     6481 gttgtcaact ctgctcaaag cagttccgac aattgacgaa ttctgcggta cagttacctt
     6541 gataagctca acaacgccct ttgagaacga atcaatttca agcgcacccg aaaatcttaa
     6601 cagacgactg atttcattag cggcggtctg ttcgggattg attgccatag aaagaccgag
     6661 atgctctttg attctgtaaa ggtcgtttgt gtattcggga ttacgcactc ttgcaattgt
     6721 gtgggtaacg cctgccgcct tagccataag acacgaatac agattgattt catcccttgc
     6781 ggttgcggca ataagcaaat cggcatactc tgcgcctgcc tcaataagca catcatatgt
     6841 tgcaccgttt ccctctatgc cgagaacatc aagcgaattt gcaagtttat cgactgcacc
     6901 gtggttaata tcgactacgg atatttcatg tccctccaaa ttaagctccg acgctattga
     6961 agagccaacc tttccgcatc caacaattat aatcctcata aattttcctc ttaaccaata
     7021 tttaaaaaca aggtgcggac cactgaaaaa attcacccgt ccgcatatca aagtcataat
     7081 acacctattt tatactaaaa tcaataccaa aatagaaaga atccgactat atacaaattt
     7141 aatttattgt gcaaaacaga agagccgagg ttctgtaccc aaaaataacg accggcgagg
     7201 actaaaccgt gtaaatcacg caagtgattg gaacggatta gacgagtgag agttattttt
     7261 ggaaacagta cagaacaacg aggcttgaca ccacgaagag ccgaggtttt gtataatccc
     7321 acaacccgtt tgggcggata atgatttcca tacgaacaaa aattttaaca taacaaaaat
     7381 tgcatcagag cctttctcag gcatctgatg caatctcttt atctaactat cttctcgaat
     7441 tgacagcggt ggcacaccgc cgaccttaat
//
LOCUS       DAOMVE010000092         7407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2638_length_7407_cov_2.902203, whole genome shotgun sequence.
ACCESSION   DAOMVE010000092 DAOMVE010000000
VERSION     DAOMVE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_7407_cov_2.902203"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..300
                     /locus_tag="PLS64_06170"
     CDS             160..300
                     /locus_tag="PLS64_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43449.1"
                     /translation="MKKEYFSPEFELVKFQFEDVILVGSLTDVPEVGDSNGDKVDGFE
                     EE"
     gene            266..1165
                     /locus_tag="PLS64_06175"
     CDS             266..1165
                     /locus_tag="PLS64_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRL43450.1"
                     /translation="MEIRLMALKRNKQFFVSIKKISAFIFAFLLVLISTFQISAASYE
                     TDGFVYSVSDGNASVTGYKGDKTDIVLPLKLQSWSVSEISDFAFVGKTAITSVRLNEA
                     RYLRRIGTDSFYGCTSLESISIPIWVRDFGAAVFQNCTSLKSVTFNCVPSKITDQMFY
                     NCTSLDKVYLPAGTSAIGKSAFAECKSLSEVYIPTSVTSIASNAFRSSPNVTIKGSYG
                     SYAEEYAKANNIPFEGISAYDVGDVNMDGGINILDATLVQKYVVGIVELSAEQKYLAD
                     YINDGDITVVDATEIQKFIVHLN"
     gene            1220..1933
                     /locus_tag="PLS64_06180"
     CDS             1220..1933
                     /locus_tag="PLS64_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43451.1"
                     /translation="MPKYLIADFIVEIEPKFDYLKKLCEPFLYEGERSADFSAIPSDS
                     YLNSLLSRMVEGSTIDEAEEFATASIFNRKIIHRGAMLVHSSALVFDGKAYLFSADSG
                     VGKSTHTKMWLKRFGSKAHILNDDKPVVKIKNEVPLCCGTPFDGGSGIANNETVPVGA
                     IIFIERSDDNFVTIPDTNEIVQRLYKSTVKFVNKSDGMSLLSNLDNLIRHTKFYVLHC
                     NTDDSAVDVAYNNIIKNCK"
     gene            complement(1981..3006)
                     /locus_tag="PLS64_06185"
     CDS             complement(1981..3006)
                     /locus_tag="PLS64_06185"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HRL43452.1"
                     /translation="MEKQNIDWANIGFGYMPIGERFVANYKDGKWDNGSMTADTTITI
                     SECAGVLQYAQTCFEGMKAYTTCDGRIVVFRPDLNGQRMEDSCKRLEMPVFPKEKFVE
                     AVCNVVKANADYVPPYGTGATLYIRPYMFGSGPVIGVKPSAEYQFRVFTTPVGPYFKG
                     GAKPITIKVSDFDRAAPHGTGHIKAGLNYAMSLHAIVTAHAEGFDENMYLDAATRTKV
                     EETGGANFIFVTKDNKVVTPKSNSILPSITRRSLMYVAEHYLGLEVEEREVYFDEVKD
                     FAECGLCGTAAVISPVGKINDHGKEICFPSGMDEMGPITKKLYDTLTGIQMGRIEAPE
                     GWIKEIK"
     gene            3287..4387
                     /locus_tag="PLS64_06190"
     CDS             3287..4387
                     /locus_tag="PLS64_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL43453.1"
                     /translation="MAEVVKIVLTGGPCGGKTSALKYISEELKKLNIPTITIGEVASR
                     LFSEGKTPENVGSYEFHRELFEIQLAEENEKTQIAKNMDCEKAVLLFDRGLLDSRAYV
                     TEDEFAKYAGIHNLNEDVIRNSYDAVFHLVTSADGAEEYYGKETNIFRREESLEKARE
                     VDTDVMAVWTGTPHLRIIDNSTDFDTKLKRLLKEVVAFLGIPKPLEIERKFLIEYPDI
                     EFLNSIKTCRRIPITQAYLTTPEEGYFRIRKRGEGDKAVYIKTVKIKISDIKRIEIEN
                     YISKEQYDSYLAQRQYVTGVISKDRYCIVDNSTYCELDVYPFWNDRATIEIELLSEDQ
                     RYQLPKFVKLIREVSSEPDYRNLALAQKYGKP"
     gene            4510..4586
                     /locus_tag="PLS64_06195"
     tRNA            4510..4586
                     /locus_tag="PLS64_06195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4544..4546,aa:Arg,seq:tct)
     gene            4593..4669
                     /locus_tag="PLS64_06200"
     tRNA            4593..4669
                     /locus_tag="PLS64_06200"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4627..4629,aa:His,seq:gtg)
     gene            4673..4748
                     /locus_tag="PLS64_06205"
     tRNA            4673..4748
                     /locus_tag="PLS64_06205"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4706..4708,aa:Lys,seq:ttt)
     gene            4814..4888
                     /locus_tag="PLS64_06210"
     tRNA            4814..4888
                     /locus_tag="PLS64_06210"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4846..4848,aa:Gln,seq:ttg)
     gene            5110..6867
                     /locus_tag="PLS64_06215"
     CDS             5110..6867
                     /locus_tag="PLS64_06215"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HRL43454.1"
                     /translation="MYIEKINGPQDVKKLSIDELNALASEMRDALLHRASVHGGHFGP
                     NFGIVEATIALHYVFDSPQDKFVFDVSHQSYPHKILTGRKEAYIAQEHYDDVTGYSSP
                     IESEHDMFTVGHTSTSVSLACGLARGRDVTNGNGNVIALIGDGSISGGEALEGFNVAG
                     EMDSNLIIIANDNQMSIAENHGGLYKNLKLLRDTDGKAECNLFKSMGLDYVYVKDGNN
                     IEELIKAFKSVKDIDHPVVVHINTLKGKGYKPAETDKESWHWCMPFDIETGKTTVNFP
                     EEEDYSSITTDYLRNKMKSDKSVVAITAGTPALYGFTPDERKAFGRQFLDVGIAEQTA
                     VAMASAIAKNGGKPVFNVYSSFIQRTYDQLSQDLCIDSNPATILVQTASVNGMTDVTH
                     LGIFDIPMISNIPNLVYIAPTTKEDYLAVLDWSIEQTDYPVAIRVPVAELVSTGKPCT
                     KNFAELNKYEVAQQGGKIAVIALGSFYGMGEQAAKLIEEKTGTAPTLINPYYITGVDT
                     ELLESLKKDHDVVVTLEDGVLDGGFGEKIARFYGTSDVKVINFGLKKEFLDRYNPADV
                     LKENRLTPEQIAEDAVALI"
     gene            6915..7235
                     /locus_tag="PLS64_06220"
                     /pseudo
     CDS             6915..7235
                     /locus_tag="PLS64_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008980360.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
BASE COUNT         2262 a         1405 c         1552 g         2188 t
ORIGIN      
        1 atatatccag tataccgatg cagatggtaa ttcggctgtt aaacttagcg atccggttta
       61 cttcacattt tatgatattg caaacaagtc ataataaatt ttaatttgta tgtgcagcag
      121 gcaactgttg cacatacaga atgagttaag gagttattta tgaaaaaaga atatttctcc
      181 cctgaatttg agcttgttaa gtttcaattt gaggatgtaa ttttagtagg aagcttaact
      241 gatgttccgg aagtaggaga cagtaatgga gataaggttg atggctttga agaggaataa
      301 acagtttttt gtttcgataa aaaagatttc tgcttttatc ttcgcatttt tattggtgct
      361 gatttctaca tttcaaattt ctgcggcttc atatgaaacc gacggctttg tatattctgt
      421 ttccgacggt aatgcgagcg taaccggata taaaggtgat aagaccgata ttgttcttcc
      481 gcttaagctt caaagctggt ctgtatcgga aatttctgat tttgcctttg tgggaaagac
      541 cgcaataaca tcggtcaggc ttaacgaagc aagatatctt agaagaatcg gtactgattc
      601 tttttacggt tgtacttcgc ttgaatcaat ttcaattcct atctgggtga gagattttgg
      661 tgctgccgta tttcagaatt gcacaagttt gaaatctgtt acctttaact gtgtgccgag
      721 taaaattacc gatcagatgt tctacaattg tacttcttta gacaaggtat atttacctgc
      781 cggtacaagt gcgattggta agagtgcttt tgctgaatgt aaatcattat ctgaggtgta
      841 tattcctaca tccgtgactt ctattgcttc taacgctttc agaagctctc cgaatgtaac
      901 aatcaagggc agttacggtt cttatgctga ggaatatgca aaggctaaca atattccgtt
      961 tgagggcatt tctgcttatg atgtcggtga tgttaatatg gatggcggga ttaatatttt
     1021 agatgcaact cttgtgcaga agtatgttgt cggtattgtt gaactttcgg cagaacaaaa
     1081 atatcttgcc gattatatca acgacggtga catcacagta gttgacgcaa cggaaattca
     1141 gaagtttatc gttcatttaa actaaccaat aaaaaaacag gctcactttt tgtgagtctg
     1201 ttttatttcg gaggacgctt tgccaaaata cttaatagct gattttatcg ttgaaattga
     1261 accaaaattt gattacttaa aaaaattgtg cgaaccgttt ttgtatgaag gggagagaag
     1321 cgcagatttt tctgcaatac cgtcagactc atacttaaat tccctgttga gccgtatggt
     1381 tgaaggttca accattgatg aagccgaaga atttgcaacg gcgtcaattt ttaaccgcaa
     1441 aataattcac cgtggcgcta tgcttgtgca ttcctcggca ctcgtttttg acggaaaagc
     1501 gtatttgttt tccgccgatt cgggtgtcgg caaatccact catacaaaga tgtggctgaa
     1561 aagattcggc tcaaaagctc atattctcaa tgacgataag cctgttgtga aaattaaaaa
     1621 cgaagtcccg ctatgctgcg gaactccgtt tgacggcgga agcggaattg ctaacaacga
     1681 aaccgttcct gtaggcgcta taatattcat agaacgctca gacgataatt ttgttacaat
     1741 tcccgacaca aatgagattg tacaaagact ctacaaatcg actgtcaaat ttgtgaataa
     1801 gtcagatgga atgtctttgc tctcaaatct cgataatctt attcggcata caaaatttta
     1861 tgtattgcat tgtaatacag atgattctgc cgtggatgtt gcctacaaca atataattaa
     1921 aaactgcaaa taaaagacgg tctgttcttg cagaccgtct tgttatatgt ataaaattga
     1981 ttacttgatt tccttaatcc agccttcggg agcttcgatt ctgcccatct gaatacctgt
     2041 aagtgtatcg taaagcttct ttgtaatcgg acccatttca tccataccgc tcgggaaaca
     2101 aatttccttg ccgtggtcgt taattttgcc gacaggtgag attacggcag ctgtaccgca
     2161 aagaccgcat tctgcaaagt ccttaacttc atcaaagtaa acttctcttt cttcaacctc
     2221 aagaccaaga taatgctcag caacatacat aagtgaacgg cgggtaattg aggggagaat
     2281 gctgttagac tttggagtaa caaccttgtt gtcctttgtt acaaaaataa agttagcacc
     2341 gcctgtttct tcaaccttag ttcttgtagc ggcgtcaagg tacatatttt cgtcaaaacc
     2401 ttctgcatga gcggtaacaa tagcgtgaag gctcattgca tagttaagtc ctgccttgat
     2461 gtgacctgtg ccgtgaggag cagctctgtc aaagtctgaa accttaattg taataggctt
     2521 tgcaccgccc ttgaagtaag gacctacagg agtagtaaat actctgaact gatattctgc
     2581 cgacggctta acaccgatta caggaccgct gccgaacata tacgggcgaa tataaagtgt
     2641 tgcgcctgtg ccgtacggag gaacataatc ggcatttgcc ttaacaacat tgcaaacagc
     2701 ctcaacaaac ttctcttttg gaaatacagg catctcaaga cgcttacagc tgtcctccat
     2761 tctctgtccg ttaaggtcag gtctgaacac aacaattctg ccgtcacagg tagtgtacgc
     2821 tttcataccc tcaaagcaag tctgagcata ctgcaaaacg cctgcacatt cgctgatagt
     2881 aattgtggtg tcggctgtca tactgccgtt gtcccactta ccgtctttat aattagctac
     2941 aaatctttca ccgatgggca tataaccgaa gccaatgttt gcccagtcaa tattctgctt
     3001 ttccatcata aatacttcct ttcaatataa gaaaaaccct cattggttat acacatattt
     3061 tagcacttta acacgcaact tgtcaatgtt ataacagcat atttcaccgt ttttatgtta
     3121 atttatacgc tttttaatgt ataatatgaa ttttactgca taagaaactt tcattttgcc
     3181 gatattatct tttaaagggc aaaaacaaac aacaatgttc ttctacagaa taaaaaaatt
     3241 gctgttggga tatatactta atttaataag ttacatgggt gattttatgg ctgaagttgt
     3301 aaaaatagta ttgacgggag gaccttgcgg tggcaagact tccgcattaa aatatatcag
     3361 cgaagagctt aaaaagctta atattcctac aattacaatc ggtgaggttg caagcagatt
     3421 gtttagtgag ggcaaaaccc ccgaaaatgt cggctcttat gaatttcaca gagaactgtt
     3481 tgaaattcag cttgctgagg agaatgaaaa aacgcaaatt gccaaaaata tggattgtga
     3541 aaaggcggtt ttgctttttg acagaggatt gcttgacagc agagcatatg ttacggaaga
     3601 tgagtttgca aagtatgcag gtatccacaa tttgaatgaa gatgtaatac gaaattctta
     3661 tgacgcagta tttcatcttg tgacttccgc agacggtgca gaagagtatt acggaaaaga
     3721 aactaatatt ttccgcagag aagaaagtct tgaaaaagca agagaagttg acacagatgt
     3781 tatggctgtg tggacaggca caccgcatct gagaattatt gataattcga ccgactttga
     3841 tactaagctg aaaagacttc tcaaggaggt tgtcgcattt ctcggaattc cgaaaccgct
     3901 tgaaattgaa agaaaatttt tgattgaata tcctgatatt gaatttttga acagcataaa
     3961 aacctgtcgc agaattccta ttacacaggc atacctcact actcccgagg aaggatattt
     4021 cagaatccgc aaaaggggag agggtgacaa ggctgtatat atcaaaactg taaaaatcaa
     4081 aatatcggat attaagcgga tcgaaattga aaattatatt tcaaaagaac aatacgactc
     4141 gtaccttgct caaaggcagt atgttacagg cgtcatcagt aaagacagat attgcattgt
     4201 tgacaacagc acctactgcg aacttgatgt ttatccgttc tggaatgaca gggcaacaat
     4261 agaaattgaa ttgcttagtg aagatcagcg gtatcaactt cctaagtttg taaagcttat
     4321 aagagaggtt tcgtctgaac ccgattacag gaatcttgca cttgctcaga aatacggaaa
     4381 gccgtaattt ggcggatttt gtccgaaaaa aactttttaa aaattaaata tgaaaaaaat
     4441 tcaaaaaagt gttgacaaat atgccgtata tgggtataat ataaaggctg actgaggtca
     4501 gcaactgatg cgccaatagc tcagctggat agagcaactg ccttctaagc agtaggtcag
     4561 gggttcgagt cccttttggc gcgccactta tggtgggatt agcgtagttg gttaacgcgc
     4621 cagtttgtgg cactggagac cgccggttcg aatccggcat tccaccccat ttgactcatt
     4681 agctcagttg gcagagcact tgacttttaa tcaaggtgtc cggagttcga atctccgatg
     4741 ggtcaccaac agccgctgaa gaaattcttt ggcggcttgt ttttttaata atcaataaaa
     4801 gtagaatata tactgagccg tcgccaagcg gtaaggcaac ggactttgac tccgtcaccc
     4861 gtgggttcga atcccgccgg ctcagccatg aaacaggcac ttgcaattat gtaagtgcct
     4921 gtttcaatta tagttatctg tcgagggtta tgctattgcg ttgcaacgat atacataaaa
     4981 aagaatacaa aagataaatt ttttaaaaat actcttgacc taaagttagc ttgaggttat
     5041 ataatatagc taaagagaga tatctctgat ttatatttaa aatttatatg ataaggagct
     5101 ctgattacga tgtatattga aaaaataaac ggaccgcagg atgttaagaa actcagtatt
     5161 gatgaactta acgctcttgc atcggagatg cgtgacgctt tacttcaccg tgcaagtgtt
     5221 cacggcggtc acttcggccc gaatttcggt attgtagagg caacaattgc ccttcattat
     5281 gtatttgatt caccacagga caagtttgtg tttgatgttt cacaccagtc atatcctcat
     5341 aaaatcctca caggcagaaa agaggcatat attgcacagg agcattacga tgatgtaaca
     5401 ggctattcaa gccccataga gagcgaacac gatatgttta ctgtcggtca tacatcaacc
     5461 tctgtcagcc ttgcctgcgg acttgcaagg ggcagagatg taacaaacgg caatggtaat
     5521 gttattgcac tcatcggtga cggctcaatc agcggcggtg aggctcttga aggttttaat
     5581 gttgccggtg aaatggattc aaacctgatt attatagcaa atgataacca aatgtcaatt
     5641 gcagaaaatc acggcggact ttataaaaat ctcaaacttc ttcgtgatac agacggcaag
     5701 gctgaatgta accttttcaa gtcaatgggg cttgattatg tatatgtcaa ggacggcaac
     5761 aatattgaag agcttatcaa ggcttttaag tctgtaaaag atattgatca tcctgttgtc
     5821 gttcatatta acactcttaa aggtaaaggt tataagcctg ctgaaactga caaagaaagc
     5881 tggcattggt gtatgccgtt tgatattgaa acaggcaaaa ctactgttaa tttccctgag
     5941 gaggaggact acagctcaat tacaacagat tatcttagaa ataagatgaa gtctgacaag
     6001 tcagttgtgg cgattacagc cggcactccg gcactttacg gctttactcc tgatgagaga
     6061 aaagcattcg gcagacagtt tcttgatgta ggcatcgcag aacagacagc cgttgcaatg
     6121 gcatctgcta tcgcaaagaa cggcggcaag cccgtattca atgtttacag ctcatttatc
     6181 cagagaacct acgatcagct ttcacaggac ttgtgcattg actcaaaccc tgcaacaatc
     6241 cttgttcaga ctgcgtcggt aaacggtatg acagatgtta cacacctcgg aatttttgat
     6301 attcccatga tttcaaacat tcctaacctt gtttatatcg ctccgacaac taaggaagat
     6361 taccttgcag tcctcgactg gagcattgag cagacagatt accctgttgc aattcgtgtt
     6421 cctgttgcag aacttgtttc aacaggcaag ccttgcacaa agaattttgc agaacttaat
     6481 aaatatgaag ttgctcagca gggcggtaag attgccgtaa tcgcactcgg ttcattctat
     6541 ggaatgggcg aacaggctgc aaagcttatt gaagaaaaaa caggcacagc gcctactctt
     6601 atcaatcctt actacattac aggcgttgac acagaacttc ttgaaagcct caagaaagac
     6661 catgatgttg ttgtgacact tgaagacgga gtgcttgacg gtggttttgg tgagaagatt
     6721 gcccgtttct acggtacgtc agatgtaaag gttatcaatt tcggccttaa aaaggaattc
     6781 cttgacagat ataatcctgc cgatgtcctc aaagaaaacc gcctcacacc cgaacagatt
     6841 gcagaagatg cagtcgcttt gatttaaata atttaaaaat aactattata aacgaatttt
     6901 aggagttaat atcaatggta aaacagacag caggaagaga taaactcggt gagtttgcac
     6961 cgaaatttgc agaaattaat gacgatgtgc ttttcggtga agtgtggagt agggaagaca
     7021 aactttcact tcgtgaccgt tcacttgtta cagttgttgc acttatgtca cagggacttg
     7081 tagattcttc ttttaagtat catcttatgt cagccaaaaa caacggcatt acaaaggaag
     7141 aaattgcaga aatcatcact cattccgcat tctatgcagg atggtcaaaa gcttgggcag
     7201 ctttcaatat ggcaaaagag gtttggacag aagaataaag agcagttgca tatatatttc
     7261 tcttgtgata caattaactg tagttatttt aaatcttaat ttttacaaag ggaaatatca
     7321 tgagaaagac taaatttata atcaccttgt taatggctgt tctgatgact ttttcatcag
     7381 ccttttgtgt aagtgtatct gcggcgt
//
LOCUS       DAOMVE010000093         7405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2640_length_7405_cov_3.711429, whole genome shotgun sequence.
ACCESSION   DAOMVE010000093 DAOMVE010000000
VERSION     DAOMVE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_7405_cov_3.711429"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..269)
                     /locus_tag="PLS64_06225"
     CDS             complement(54..269)
                     /locus_tag="PLS64_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HRL43455.1"
                     /translation="MKMTNKIYDVLKYIALIVLPAIGTLYFAVAGVWGLPYGEQIVGT
                     ITAVDTFLGALLGLSAYKYNKTDESEE"
     gene            complement(283..558)
                     /locus_tag="PLS64_06230"
     CDS             complement(283..558)
                     /locus_tag="PLS64_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRL43456.1"
                     /translation="MKIDIVQLAEIISALALIGGVVFGVFKFIENNKKQNAEIKKIKG
                     EQTLTMYALRACLDGLKQQGCNGRVTEAINKIDKYLNQSAHSAEDLN"
     gene            complement(562..810)
                     /locus_tag="PLS64_06235"
     CDS             complement(562..810)
                     /locus_tag="PLS64_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43457.1"
                     /translation="MELKEKITLDMLTKDSVSVLRQQFLTFNGEEMQVGGNIRNAYMN
                     SKTGREQLKTVLSDEYYNAVMAVWGDNPTVDEPTESEV"
     gene            complement(831..1886)
                     /locus_tag="PLS64_06240"
     CDS             complement(831..1886)
                     /locus_tag="PLS64_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43458.1"
                     /translation="MIIYDKRKPKTFDGANCVGITGENLAETQEFLIRGMCDITVDYF
                     IHLRFADGSVNSVIPDSTAIDETGTKLVWKVRKNDIFMHGYFELQLEGRGENGYVFQT
                     QIVTLYADESIPIEDKEYLNPNSETLKLREETQKLLDETKLCRTEIDKTKKLIEQSDI
                     TKKEDNSNKVSDSSQITDYTQNYPSIKYLTDNYWDSADSYSSDEVDILLNAKADNKPL
                     NERLARMDMSIGSKYDSSNIELGTATLTPYSTQIDKIKSATCLYEKIGDIVIVNVTVI
                     MNATSLGGTSTIALLNMPFSNKSDVIVHDIGISKNGGMFRGSVNKSAWLQFTPLNKQA
                     YNFVADEQVNFSLIYKI"
     gene            2188..2895
                     /locus_tag="PLS64_06245"
     CDS             2188..2895
                     /locus_tag="PLS64_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRL43459.1"
                     /translation="MRKYVEVFTRDHLPKLRDQLLFSGLTDHEIFMFIQYAKPYYLSL
                     NRGETARVENKFSHMTGLVFSGTAYLYSVDYNGNKSLLKTIRSGESSGTLYAMFDYYN
                     TLVEIMAGEDCEILFIPPEPMFITEDSLASIQQKILVNMLASQRQVFLQISEHLACLS
                     KRSIKGKFMHLLQIYCRRERSCEVDLPFSRDELANYLAVDRASLSRALGELKKAGIIE
                     FKKSHFKLIETAEYHFD"
     gene            2963..3526
                     /locus_tag="PLS64_06250"
     CDS             2963..3526
                     /locus_tag="PLS64_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRL43460.1"
                     /translation="MSFVELLLFGIGLSMDAFAVSICKGLSVRKLRPKHAITVGLWFG
                     GFQALMPLIGYLLGKQFEWLITSIDHWIAFILLALIGANMIREAFGEEEQLDDSFSVK
                     TMLPLAVATSIDALAVGVSFACIDVDIVPAIAIIGTTTFILSAVGIKIGNVFGAKYKS
                     KAEFLGGAVLILLGLKILLEDLGLLPF"
     gene            complement(3697..4650)
                     /locus_tag="PLS64_06255"
     CDS             complement(3697..4650)
                     /locus_tag="PLS64_06255"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003501700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HRL43461.1"
                     /translation="MSGNKRKVVLVGTGMVGMSFAYAALNQNVCDELIMIDLNEKRAE
                     GEAMDLNHGLAFSHSSMKIRCGGYGECADADIVVICAGANQKPTESRLQLLQKNAVVF
                     SSIVPKVVQSGFEGIFLVATNPVDIMTRVTYELSGFNASKIIGTGTTLDTARLRYLLG
                     EYFEVDPRNIHAYVIGEHGDSEFVPWSQALLAAKPLKDVMRDNPKSYYMEELEQISDD
                     VRCAAHKIIEAKGATYYGIGMSIVRIVRAILQDENSVLTVSVRLRGEYGGKKDVFIGN
                     PCIVSANGAKRILELKLTEQELQKLDNSCTILNDNFKKLKL"
     gene            5066..5908
                     /locus_tag="PLS64_06260"
     CDS             5066..5908
                     /locus_tag="PLS64_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HRL43462.1"
                     /translation="MYKIVKKKSLNPTVTLMEIDAPLIAKKAEPGQFIILRVDENGER
                     IPLTVAGYDREAGTVRIIFQIVGATTEKLNHLNEGDYIHDFVGPLGVPTHTDGLKKVC
                     VVGGGVGCAIALPVAEKLHEIGCEVHSIVGFRNKDLVILEDEFKACSDKFILMTDDGS
                     YGQKGVVTAPLEEMIKAGEDYDLVITIGPLIMMKFVVKTTKPYNIPTTVSMNPIMIDG
                     TGMCGGCRLTVDGQTKFACVDGPDFDGFKVDFDEAMKRGAMYKPFERHAYEETCNLFK
                     KEVE"
     gene            5911..7305
                     /gene="gltA"
                     /locus_tag="PLS64_06265"
     CDS             5911..7305
                     /gene="gltA"
                     /locus_tag="PLS64_06265"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HRL43463.1"
                     /translation="MPNMSPKKAPMPSQDPNVRNKNFLEVATGYTEEVAIEEAQRCLN
                     CKHRPCVNGCPVHIAIPDFIKEVAQGNFAGAYDVISQSSSLPAVCGRVCPQESQCESK
                     CVRGIKTEPVGIGRLERFVADWHNAQTDVKVNKPESNGHKVAIVGSGPAGLTCAGDLA
                     KKGYDVTVFEALHLAGGVLVYGIPEFRLPKSIVQKEVDTLKELGVKVETNMVIGRVLS
                     IDELMDDYGFESVFIGSGAGLPRFMNIPGENLCGVYSANEFLTRTNLMKAYKDDSTTP
                     IMHAKKVAVVGGGNVAMDAARCAKRLGAEEVYIVYRRSEEELPARKEEVEHAKEEGII
                     FKLLNNPVEILGNEDEFVTGMKCIKMELGEPDASGRRRPVEIPGSEFVLDVDAVVISI
                     GTSPNPLIKATTKGLDTQKWGGIIADENGLTSREGVYAGGDAVTGAATVILAMGAGKT
                     AAAAIDEYIRNKNK"
BASE COUNT         2001 a         1529 c         1680 g         2195 t
ORIGIN      
        1 ttctttgtaa tcttcgctta acattttgtc aacagtatta gataaaacca taattattcc
       61 tcgctttcgt ctgttttgtt atatttataa gctgacaagc cgagcagagc gcctaagaag
      121 gtatcaacgg ctgtgatagt gcctacaatc tgttcgccgt atggcaagcc ccaaacgcct
      181 gctacggcaa agtaaagtgt accgattgca ggcagtacga taagagcaat gtatttaagt
      241 acatcataga ttttgtttgt cattttcatt attatcatcc tttcaattta aatcttccgc
      301 cgaatgtgcc gactggttga ggtacttatc aatcttattg attgcctcgg tcactcgacc
      361 gttgcaaccc tgctgtttta agccgtcaag acaagcacgc agagcgtaca tagtcaaggt
      421 ctgctcgcct ttgatttttt tgatttcagc gttctgcttt ttgttgtttt cgataaattt
      481 aaaaacacca aatacaacac cgccaattaa ggctaacgca gatatgattt cggcaagctg
      541 tacaatatca atcttcatcg cttacacctc gctttctgtc ggctcatcaa cggttggatt
      601 atcaccccat actgccatga cggcattgta atattcatca gacagcaccg ttttgagctg
      661 ttctctgccc gtcttgctgt tcatgtatgc gttgcggatg tttccgccga cctgcatttc
      721 ttcaccgtta aaggtcaaaa actgctgtct gagtaccgac acgctgtcct ttgtgagcat
      781 atcaagtgtg attttttctt taagttccat ttttcatacc tccgttattt ttatattttg
      841 taaatcaaag aaaagtttac ctgctcatca gcgacgaaat tataagcctg tttattgagc
      901 ggagtaaact gcaaccaagc cgatttattt acacttcctc tgaacattcc gccgtttttg
      961 cttatgccga tatcatgaac aatcacatcc gatttgtttg agaaaggcat attgagcaaa
     1021 gctattgtag atgttccgcc taaagatgtt gcgttcataa tgacggtgac atttacaata
     1081 acgatatcgc caattttttc ataaaggcaa gttgcagatt ttattttatc aatctgagta
     1141 gagtacggag taagagtagc tgtgccgagt tcgatatttg acgaatcgta ttttgagccg
     1201 atgctcatat ccatacgggc taatctttca ttaaggggtt tgttgtcagc ttttgcgtta
     1261 agcagaatgt caacctcatc cgacgagtag ctgtcggcac tatcccagta gttgtcggta
     1321 agatacttga tactcggata attttgagta tagtcggtaa tctgagagga atcacttacc
     1381 ttgtttgagt tgtcctcttt ttttgtaatg tcggactgtt caattaactt tttggttttg
     1441 tcaatttcag tccgacaaag ctttgtttcg tcaagaagct tttgcgtttc ttcacgaagc
     1501 ttcagcgttt ctgagttcgg attaaggtac tctttgtcct caatcggaat actctcgtct
     1561 gcataaagcg tgacgatctg agtttggaaa acatagccgt tttcgcctct gccttcaagc
     1621 tgaagttcaa aatagccgtg catgaaaatg tcgttttttc tgaccttcca cacaagtttt
     1681 gtgcctgttt catcaattgc ggtgctgtcg ggaattaccg aatttacact gccgtcggca
     1741 aaacgcagat gaatgaagta gtcaaccgta atgtcgcaca ttccccgaat taaaaattcc
     1801 tgtgtttcgg caagattttc acccgtgatg ccgacgcagt ttgcaccgtc aaaagttttt
     1861 ggttttcgtt tgtcatagat aatcattgtt acacctccac ttaaagaggc aaaaacacga
     1921 aaaaatgcat tgttttatgc aattttcaga aaaatttttg aaattttttt aattttccgt
     1981 cacgaggggg aaacggttgc aattgcaatg ttttgtgata taatctaatt tgaataattt
     2041 gaacgaattg acttaaacag aagagaagaa aaacggtttg ttccgtgacg attaagagga
     2101 cggaattgtg tgccgatgta caaattacgg cttttttcgt tgcaaaccgt ttgaacgaag
     2161 ggggacggca acacgccgtg atataaaatg agaaaatatg tagaagtttt tactcgtgat
     2221 catttgccaa agctgaggga tcagttgttg ttttcgggat tgacggatca cgaaattttt
     2281 atgttcatac aatatgcaaa accgtactat ctgtcgctga accgtggcga aacggcaagg
     2341 gttgaaaaca agttcagcca tatgacggga cttgtgtttt cgggaactgc atacctttat
     2401 tctgtggact acaacggcaa caaaagtctg ctcaaaacaa ttcgcagcgg cgagtcaagc
     2461 ggaacgcttt acgcaatgtt tgactactac aatactcttg ttgaaattat ggcaggagag
     2521 gactgcgaaa ttctttttat tccgccggag ccgatgttta taaccgagga ttcgcttgct
     2581 tcgattcagc agaaaatcct cgtcaatatg cttgcgtcac aaagacaggt gtttttgcag
     2641 atttcggaac accttgcctg tctttcaaag cgaagtatca agggcaaatt tatgcacctt
     2701 cttcaaatct attgcaggcg tgagcgtagc tgtgaggttg atctgccgtt ctcccgtgac
     2761 gagcttgcaa actatcttgc ggttgacaga gcgtcacttt caagagcact cggtgaactg
     2821 aaaaaagcag gaattattga atttaaaaag agccatttca aactgattga aacagctgaa
     2881 tatcattttg attaaataac ggtcggcaaa cggtgttctg tttcctttca ccgccgacta
     2941 aaatataacg gaggataata ttatgagttt tgtggaatta ctgctgttcg gcatcggctt
     3001 gtctatggat gcatttgcgg tttcaatttg caaggggctc tccgtgcgta agttaagacc
     3061 taaacacgca attactgtcg gattgtggtt tggcggattt caggcgctta tgccgcttat
     3121 cggttatctt ctcggaaagc agtttgagtg gctcatcaca agcattgacc attggattgc
     3181 attcatcctg cttgcactta tcggtgcaaa tatgataaga gaggctttcg gtgaggaaga
     3241 acagcttgac gattcgtttt cggtcaagac aatgctgccc cttgccgttg caacaagtat
     3301 tgacgcactt gcggtaggcg tttcgtttgc gtgtattgat gttgatatcg tgcctgcaat
     3361 cgcaattata ggcacaacaa cattcatact ttcggctgtc ggcatcaaaa taggcaatgt
     3421 attcggtgca aagtataagt caaaggcaga atttttaggc ggtgcagtat tgattttgct
     3481 tggactcaag attttgcttg aagatttagg gcttttgccg ttctgacgaa tttcaaattg
     3541 aaagttgacg gcgagccgcc gttaccaatt cgagaagata gtataatata gaaatcgcat
     3601 cagatgcccg aaaaagctct gatgcgattt tttatataaa agtcgttgct gtttcgtagg
     3661 gcggatatta tccgtcctac agacaatacg gggtttttat aatttcaatt tcttaaagtt
     3721 gtcattcaga attgtgcagg agttatctaa cttttgcaat tcctgttcgg ttaatttaag
     3781 ttccagaatg cgttttgcac cgtttgcgct tactatgcac gggttgccga tgaacacatc
     3841 tttcttgccg ccgtattcgc cacgcagacg aaccgatact gtcagaacgc tgttttcatc
     3901 ttgaagaatt gctcggacga tacgaacaat tgacatacca atgccgtagt aggttgcgcc
     3961 ctttgcctca atgattttgt gggcggcaca acgcacatca tcgcttatct gctcaagctc
     4021 ctccatgtag tacgattttg ggttatcacg catgacatcc tttagcggtt tggcggcaag
     4081 gagtgcctga ctccacggaa caaattcgct gtctccgtgt tcgcctatta catatgcgtg
     4141 aatgttgcgt ggatcaacct caaaatattc gccgagaaga tatctgagcc gtgccgtgtc
     4201 aagtgtagtt cccgtgccga tgatttttga cgcattgaat cccgaaagtt cataggtcac
     4261 ccgtgtcata atgtcaacgg gatttgtcgc aacaaggaaa attccctcaa aaccgctttg
     4321 cacaaccttt ggcacaattg aagaaaatac aactgcgttt ttctgcaaaa gctgaagcct
     4381 gctttcggtg ggcttttgat ttgcacctgc gcagattaca acaatgtcgg cgtcggcaca
     4441 ttcaccgtaa cctccgcaac ggattttcat gcttgaatgc gaaaatgcaa gcccgtggtt
     4501 caaatccatt gcctcgccct cggcacgctt ttcgttgagg tcaatcataa tgagctcatc
     4561 acacacattt tgattgagtg ccgcataggc aaagctcatt ccgaccattc ccgttcccac
     4621 aagtacaacc tttcttttgt ttcccgacat atgaccgttc ctttcatttg tattcttttt
     4681 ttaggatttt catatgtgca aaaattattc ttcggttcaa aaaattgatt ggattgtgct
     4741 ttttgggggg ttgtgaaaaa aataacggca gaatcggctg tttgtttggt gaaatggcaa
     4801 agattatatg taaaaatcat caaaattcac ttgaaacgca gatataattg tgctaaaatt
     4861 aattcgacaa ggtgttcgga atacattagg catattccga tttttatttt ataggaaaat
     4921 gtccgacaat gctcggacgg aaaggtctga taattcagac ttttcatatt gttatttcag
     4981 tcatttcaag tttgcacttg aaatgattgg cgaacactgt tcgccgctac tgatgtgaat
     5041 tcaattgcga aaggaaaaga gaaaaatgta caagatagtt aagaaaaaat ctctgaatcc
     5101 aaccgttaca cttatggaaa ttgacgctcc gcttattgcc aaaaaggcag agccgggaca
     5161 gttcatcatt ctccgtgttg acgagaacgg tgagcgtatt ccgcttacgg ttgcaggcta
     5221 tgacagagag gccggcacag ttcgtattat cttccagatt gtcggtgcaa caacagaaaa
     5281 gctcaatcac cttaacgaag gcgattatat tcacgatttt gtaggccctc tcggtgttcc
     5341 cacacataca gacggtctta aaaaggtttg cgttgtaggc ggcggcgttg gctgtgcaat
     5401 tgcacttcct gttgccgaaa agctccacga aatcggctgt gaggttcact caattgtcgg
     5461 tttcagaaac aaggatcttg ttattcttga agatgaattc aaggcttgct cagacaagtt
     5521 tattcttatg acagatgacg gttcatacgg tcaaaagggc gttgttacag ctcctctcga
     5581 agagatgatt aaggcaggcg aggactatga cctcgttatt acaatcggtc cgcttatcat
     5641 gatgaagttt gttgttaaaa caacaaagcc ttacaatatt cccacaacag tttccatgaa
     5701 cccgattatg attgacggca caggtatgtg cggcggctgt cgtcttacag ttgacggaca
     5761 gacaaagttt gcctgtgttg acggccccga ctttgacggc tttaaggttg attttgacga
     5821 ggctatgaag cgtggcgcaa tgtacaagcc gtttgagcgt catgcatacg aagaaacatg
     5881 taatctgttc aagaaggagg ttgagtaatc atgccaaata tgtcaccaaa aaaggctcct
     5941 atgccatcac aggatcctaa tgtaagaaat aaaaactttc ttgaggtagc aacaggctac
     6001 acggaagaag ttgcaattga ggaggcacag cgttgcctta actgtaaaca cagaccttgc
     6061 gtaaacggtt gtccggtaca tatcgcaatc cccgatttta tcaaagaggt tgcccagggc
     6121 aactttgcag gcgcttatga tgtaatttca cagtcaagct cacttcctgc tgtttgcggc
     6181 cgtgtttgtc cgcaggagtc acagtgtgag tcaaagtgcg taagaggcat caagactgag
     6241 cctgtcggaa tcggcagact tgagagattt gttgccgact ggcacaacgc tcagacagat
     6301 gttaaggtaa ataagcctga gtcaaacggt cacaaggttg ctatcgtcgg ttcaggtcct
     6361 gcaggtctta cctgtgcagg cgaccttgca aagaagggtt acgatgtaac agtatttgag
     6421 gctcttcacc ttgcaggcgg tgttctcgtt tacggtatcc ctgagttcag acttcccaag
     6481 tcaatcgttc agaaggaagt tgacacactt aaagagctcg gtgttaaggt tgaaacaaat
     6541 atggttatcg gcagagttct ctcaattgac gagcttatgg acgattacgg ctttgagtca
     6601 gtatttatcg gttcgggcgc aggtcttccg agatttatga atatccccgg tgagaacctt
     6661 tgcggtgttt actcggcaaa tgagttcctc acaagaacaa accttatgaa ggcttataag
     6721 gatgacagca caactccgat tatgcacgca aagaaggttg cagttgtcgg cggcggtaat
     6781 gttgcaatgg atgccgctcg ttgtgcaaag cgtcttggcg cagaagaggt ttatattgtt
     6841 tacagaagaa gcgaagaaga gcttcctgcc cgtaaggaag aagttgagca tgcaaaggaa
     6901 gagggaatta tcttcaagct cctcaataac cctgttgaaa ttctcggcaa cgaagatgag
     6961 ttcgtaacgg gtatgaagtg cattaagatg gaactcggcg agcctgacgc aagcggcaga
     7021 agaagacctg ttgaaatccc cggcagtgag ttcgttcttg atgttgacgc tgttgttatt
     7081 tcaatcggta catcacctaa cccgcttatc aaggctacaa caaagggact tgacactcag
     7141 aagtggggcg gtatcatcgc tgacgaaaac ggtcttacat cccgtgaggg cgtatatgca
     7201 ggcggtgacg ctgtaacagg cgccgcaacg gttatcctcg caatgggtgc aggcaaaaca
     7261 gccgcagccg ctattgacga atacatccgg aataaaaata agtaattttg ttctgagttg
     7321 aaaatggaaa attgaaaatg taaaattacg gtcggcggtg tgccaccgct gtccattcga
     7381 gaagatagtt agataaagag attgc
//
LOCUS       DAOMVE010000094         7319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2683_length_7319_cov_2.995182, whole genome shotgun sequence.
ACCESSION   DAOMVE010000094 DAOMVE010000000
VERSION     DAOMVE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2683_length_7319_cov_2.995182"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    87..96
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    580..589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(680..1066)
                     /locus_tag="PLS64_06270"
     CDS             complement(680..1066)
                     /locus_tag="PLS64_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43464.1"
                     /translation="MTNTTDSNVQNVTVNNSANSDSSTNVEYSQNFVQVNPKKKPAPI
                     KIVIPVVILCVLAAVLIAPLIDANPVVSNRLVGKCKNSNETTYVFNENGIQYYYNLHT
                     LVINAFSDDFKDATFDRIHLSESDLQ"
     gene            complement(1224..2114)
                     /locus_tag="PLS64_06275"
     CDS             complement(1224..2114)
                     /locus_tag="PLS64_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43465.1"
                     /translation="MNCKRKIHEKLVFENAPQNPEAIKGIRAYLLALLSYATMSKRCE
                     NADFDLHIDIEGDETLFETEDESAGEIIQKLGNHGGFTIDITCSGKFVNIGNCSDEEF
                     ITVACFPDRVPSIFDCLENSGEKDFKYGKIAYFDGIDLWLSQYIDDKTCMDNVFDFGD
                     IVEKKDFAISNLWNSEMFLVIAFDFEKYHDEAEEIKNIIERYIYVDDEKAFKKCRKKW
                     DEEMIIGIDGVQWIPENVGEIVAFTDEINEVLARLDRDEDVEIESYGLFCNMDEFFSA
                     QIYADNEEHCFKVVAVQSVV"
     gene            2374..3729
                     /locus_tag="PLS64_06280"
     CDS             2374..3729
                     /locus_tag="PLS64_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRL43466.1"
                     /translation="MERENFKSRLGFILVSAGCAIGIGNVWRFPYVAGQNGGGLFVLL
                     YLIFLVLMGVPVLTMELAVGRASRKSAVLAYKKLEKPKSKWHIHGWFCMLGCYLLMMY
                     YTTVSGWMTSYFYKFATGSFESGMTSEQVGGVFNELQSNPLEMVIWMAIITVLGFLVC
                     SRGIQKGIEKVSKVMMIALLILILVLAGNSLFLSGAGEGLSFYLIPDLDKVNEIGLGN
                     VISSAMNQAFFTLSLGIAAMEIFGSYMSKDHTLPSESIKICALDTFVAIMAGLIIFPA
                     CFSYGVQPDQGPALIFVTLPNVFVNMAGGRIWGTLFFLFMTFACFSTIIAVFENIISF
                     WIDMFGISRKKSVLINTIIILIASLPCVFGFNIWSGFQIFGQNVLGMEDFLVSNILLP
                     VGSLVYLLFCVTKFGWGFDNYLDECNTGKGIKFSKALKPYFKYVLPVLVLILIVQGFI
                     K"
     gene            complement(3812..4234)
                     /locus_tag="PLS64_06285"
     CDS             complement(3812..4234)
                     /locus_tag="PLS64_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRL43467.1"
                     /translation="MKKTKLLKIVLKRTGAIKLLCSYVIVFFAVSVAIWIFEPNITRL
                     FDSIWYCFSVATTIGLGDVLATTLVGRVLSIFLSICSILIIAVVPGVITSYYVESTKL
                     RADESSAKFLDDLEHLPELSKEELVELSEKVKKFNRKK"
     gene            complement(4448..5050)
                     /locus_tag="PLS64_06290"
     CDS             complement(4448..5050)
                     /locus_tag="PLS64_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HRL43468.1"
                     /translation="MADILYVYKTSIYANLTNKCPCRCTFCIRNNTDSVGSADTLWHK
                     HDPSMEDIKKAVDDFDFTGYKELVFCGYGEPTSALDNMLETAKYVREKHPDIKLRLNT
                     NGLSDRINNKPTAELISKYIDSVSISLNTCSSEKYDEVCRPVFDHAYESMLKFAEDAK
                     KYFEHTQFSIVDTIPEEDIKACQKIADDRGIYLKIRKYSD"
     gene            complement(5081..5632)
                     /locus_tag="PLS64_06295"
     CDS             complement(5081..5632)
                     /locus_tag="PLS64_06295"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRL43469.1"
                     /translation="MTAKTVSKTDSKYNVKLIAITAMCAALVTVTTAFIKIPSPLGYS
                     HAGDSMIYLAASILPGPFGIIASSIGGALADLISGYPHWAIPTAIIKAVNAVPFVLCR
                     MAMKKRGKDEKIISPSSLLMLIPTTIVTIGGYFIANGLMYDFGAAIAELPTCWLQPGV
                     GALIYIVVGIGLDKINFKKKLSL"
     gene            complement(5643..6497)
                     /locus_tag="PLS64_06300"
     CDS             complement(5643..6497)
                     /locus_tag="PLS64_06300"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HRL43470.1"
                     /translation="MMTKKAVLINDLSGLGKCSLTAAISVLSVMGIQACPMPTAVLTS
                     QTGFESFYCNDCTDKLDYYTSEWKKLGFQPDGIYTGFLSSEKQVDKILSFIDSFETDN
                     TLVLVDPVLGDGGKTYSLYTETLGKKMKKLVSVADVITPNLTECCILCDTDYSELIEN
                     ADSPDFLDTVAELAKKLLKLYKVKTVIVTGIEHKISDEVTKINNLIAADDEVFCVSCE
                     KTGGSYSGTGDLFASVVFSGLLRGDSLKDTVNLAVKFIQTSLCETVKLGIHRNEGIEF
                     EKHLKMLL"
     gene            complement(6677..6931)
                     /locus_tag="PLS64_06305"
     CDS             complement(6677..6931)
                     /locus_tag="PLS64_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009532726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HRL43471.1"
                     /translation="MITKSAQKDKEKIKQYPALKRNVQNLLELISENPFQNPPPYERL
                     IGNLKQCYSRRINSQHRLVYMVYEEEKIVKIISMWSHYEF"
     gene            complement(6942..7181)
                     /locus_tag="PLS64_06310"
     CDS             complement(6942..7181)
                     /locus_tag="PLS64_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRL43472.1"
                     /translation="MINTNVTNFRKQIFELIDQTVKYNEPINISTKSGNAVLISEEDY
                     NNLVETMYLNSIPGMSEKLKDGIETPLEECEEFEW"
BASE COUNT         2098 a         1487 c         1374 g         2340 t
ORIGIN      
        1 accgacaagg ctgttatgag caagactttt cttgaactct gcgttatggg caatattgat
       61 tgcgatgagc ctgatatgat tgatttnnnn nnnnnngagc ttatcatttc tcttgaaaag
      121 cactttgacc ttgaactaac cgacaaggct gttatgagca agacttttct tgaactctgc
      181 gttatgggca acattgattg cgatgagcct gatatgattg attttgagat ttacagggtt
      241 gtgaggaatt caaagagcga ggcgctttat gcttgcgaaa aatacgacct cgatgatgac
      301 gacattttta taatttttga ggagcataac aacttcttta tcagtaactc gcagattgtc
      361 gagatgctgc ttatcatcaa agagggtgtt tctgccgagg actatgagaa tgaaactccc
      421 gaatttctca actacatccg tgtctgccgt tacttctgcc agacaaactt ttttaagctc
      481 aacaaggctg ttacatatga cgagttcgat atgattgaca ttccagaaga tgaaagcgat
      541 tctgacgatg ccgattatta tgacgagtct gatgatgaan nnnnnnnnnt tgagttttaa
      601 tttttgaatt ttcacttttt aactctaaca aaatagcaaa aaaattacga ggaaggtttt
      661 atgccttcct cgtttttgtt tactgcaaat cactttctga aagatgaatt ctatcaaaag
      721 tagcgtcttt aaaatcgtct gaaaatgcat ttattaccaa ggtatgtaag ttgtagtagt
      781 actgaatacc attttcatta aatacatatg ttgtttcgtt gctgttctta cattttccaa
      841 ccaacctgtt agaaacaaca ggatttgcgt caatcaatgg tgcaatcaaa acagccgcca
      901 aaacacataa tatcactaca ggtatcacta tttttattgg agcaggtttc tttttcggat
      961 taacctgaac aaaattctga gaatattcaa catttgtact actatctgaa ttagcagaat
     1021 tatttacagt cacattttga acattactgt ctgttgtgtt tgtcacaggt tcagttgaac
     1081 tattaacact gtcacttggc tctgccgcag aaacaaatta ttcaagaaac gagcttatca
     1141 atgtaatcct cagtcacggc gttgaaaaca tcataataga ataatcaaac tttttatcgg
     1201 gaaggagaaa tccttccctt ttttcatact acggactgaa ccgcaacaac tttaaagcaa
     1261 tgttcctcat tgtctgcata aatctgcgcc gagaagaact cgtccatatt gcagaaaagt
     1321 ccatacgatt caatttctac atcttcatcc ctgtcaaggc gagcaagcac ttcgttgatt
     1381 tcatcggtaa atgcaacgat ttctcccaca ttttcgggaa tccactgcac accgtcaatt
     1441 ccgattatca tttcctcatc ccactttttg cggcactttt taaatgcttt ttcatcatca
     1501 acataaatat atctttcgat tatattcttg atttcctcag cttcatcgtg atacttttca
     1561 aaatcaaatg cgataacaag aaacatttca ctattccaca aatttgaaat tgcgaagtcc
     1621 tttttctcaa caatgtcgcc aaaatcaaaa acattgtcca tacaggtttt atcgtcaatg
     1681 tactgggata accacaaatc aatgccgtca aaataagcta tttttccgta cttgaagtcc
     1741 ttttcgccag aattttcaag gcaatcaaaa atgcttggaa ctctatcagg aaaacacgcc
     1801 accgtgataa attcctcatc ggaacagttg ccgatattca caaattttcc gctgcaggtg
     1861 atgtcaattg tgaatccgcc gtgattaccg agcttctgga ttatctcgcc cgcactttcg
     1921 tcttcggttt caaaaagcgt ttcatcgccc tcaatatcaa tatgcaaatc aaagtcagcg
     1981 ttttcacatc tcttcgacat cgttgcatag gaaagcagcg ccaaaaggta tgcacggata
     2041 cccttgattg cttcgggatt ttgcggtgcg ttttcaaaca caagcttttc gtggatcttt
     2101 cttttacaat tcattaacat aataatttcc gcctttcatt aacttagtaa caattattgt
     2161 tattgccctt ttaattaggt ttttcctttc cttttcttta ataatattat accataaacc
     2221 actgtcccat tatctaccct ctgctctaaa aaacggctta tttatgcggt ttttttgcga
     2281 aaattcttca tttattaaaa acagagcatc ttgacaaatg tgtgaatttt ttgtacaatt
     2341 taaaagtctg tttttatagg agagaattcg tttatggaaa gagaaaattt taaatcccga
     2401 cttgggttta tccttgtaag tgcgggttgc gctatcggaa tcggcaatgt gtggcgattt
     2461 ccgtatgttg caggacagaa cggcggcgga ctatttgtac ttttgtacct aatcttcctc
     2521 gttcttatgg gagttcctgt tttgacaatg gaacttgcag tcggaagagc gagcagaaaa
     2581 agtgctgttc tcgcctacaa aaaacttgaa aagccaaaga gcaagtggca cattcacggc
     2641 tggttctgta tgctcggatg ctaccttttg atgatgtact acaccacggt ttcgggttgg
     2701 atgacgagct acttctacaa atttgcgacg ggttcatttg aaagcggaat gacctcggag
     2761 caagttggcg gagtattcaa cgaattgcag tcaaatccgc ttgaaatggt aatttggatg
     2821 gcgattatca ccgtcctcgg attccttgtt tgcagcagag gtattcaaaa gggcattgaa
     2881 aaagtcagca aagttatgat gattgcgttg ctcatactca ttcttgtact cgcaggcaac
     2941 agcttattcc tttcgggtgc aggcgaggga ctcagcttct accttattcc cgatttggac
     3001 aaggtgaatg aaatcggact tggcaatgtt atttcatccg ctatgaatca ggcattcttc
     3061 acactcagcc ttggcattgc ggcaatggaa attttcggca gttatatgtc aaaagaccac
     3121 actctgccga gtgagtcaat caaaatttgc gcacttgaca catttgttgc gattatggca
     3181 ggtcttatca tcttccccgc ttgtttttca tacggtgttc agcccgacca aggccctgct
     3241 ctgattttcg taacacttcc gaatgtgttc gtaaatatgg ccggcggaag aatttggggt
     3301 actctgttct tcctgtttat gacttttgcg tgcttctcaa caatcattgc cgtgtttgaa
     3361 aacatcattt cgttctggat tgatatgttc ggcatcagca gaaagaagtc tgttcttatc
     3421 aacacaatca tcattcttat cgcaagtttg ccgtgtgtgt tcggtttcaa catttggagc
     3481 ggtttccaaa ttttcggtca gaatgttctc ggtatggaag attttctcgt aagcaatatc
     3541 cttcttcctg taggttcgct tgtgtatctc ctcttctgcg ttacaaaatt cggttgggga
     3601 tttgacaact accttgatga gtgtaacacc ggtaaaggca ttaaattttc aaaggcttta
     3661 aagccgtatt tcaagtatgt actccctgtt cttgtactga ttttgattgt tcagggattc
     3721 ataaaataag ttaatatatt tctttaaaag caaaatcccg acagtatgtt tttgatactg
     3781 tcgggatttt ttatgtccgc atttattaaa tttacttttt acgattaaac tttttcacct
     3841 tttcggaaag ctcgacaagc tcttcttttg aaagttcggg aagatgttca aggtcatcaa
     3901 gaaactttgc actgctttca tcagcacgaa gttttgtgct ttcgacatag tagcttgtga
     3961 ttacgcccgg aactactgca atgataagaa ttgaacatat ggaaagaaaa attgaaagca
     4021 ctctgccgac aagcgttgtt gcaagcacat ctccgagtcc tattgttgtg gcaacgctga
     4081 agcagtacca tatgctgtca aaaagccgtg ttatgttcgg ctcaaaaatc catattgcaa
     4141 cactcaccgc aaaaaacacg ataacataac tgcacaaaag cttgattgca cccgtccgct
     4201 tgagaacaat tttcaacagt tttgtctttt tcaaagcacc accccatttg tataaattat
     4261 agcaattaaa attacctccg tcaaggcggt ggatatacag ctttaagaat agaatcaatt
     4321 gaaagtgaaa ttgtcctata ggacagttgc caatgacata ccgaataaaa ataaaaccga
     4381 cctgtcataa caacaagtcg gctttacttc tgaaatataa aatgaaatga aaaattaaaa
     4441 ttaacgatta atcggaatat tttctgattt tgagataaat tcccctgtca tcggcaattt
     4501 tctgacaagc cttgatgtcc tcctcgggga ttgtatctac gattgaaaac tgcgtatgtt
     4561 caaaatattt ctttgcatcc tctgcaaatt tgagcattga ctcatacgca tggtcaaaaa
     4621 caggacggca aacttcgtca tacttttccg atgaacaggt attgaggctg attgaaacag
     4681 aatcaatata ctttgaaatc aactcagctg tgggcttgtt gttgattctg tcagaaagac
     4741 cgtttgtgtt gagtctgagt ttgatgtcgg ggtgcttttc ccttacatat tttgcggttt
     4801 caagcatatt atcaagagcc gaagtcggtt cgccgtagcc acagaataca agctccttgt
     4861 aacctgtgaa atcaaaatca tcaacagcct ttttaatatc ctccatactc ggatcatgct
     4921 tgtgccagag tgtatctgca ctgccgaccg aatcggtatt gttgcggata cagaatgtgc
     4981 atctgcaagg gcatttgttc gtcagattag catagataga tgttttgtac acataaagaa
     5041 tatctgccat tatgttttct tctccttatt gaaactcaca ttaaagcgag agttttttct
     5101 tgaaattgat tttatcaaga ccgatgccta caacaatgta gattaatgct cctacaccgg
     5161 gctgcaacca gcaagtggga agttccgcaa ttgcggcacc aaagtcatac ataagaccgt
     5221 ttgcaataaa gtaaccgccg attgtaacga ttgttgttgg aatgagcata agaagtgatg
     5281 acggactgat gattttttca tctttgccac gctttttcat tgccattctg caaagcacaa
     5341 acggaactgc gttgacagcc ttgataatag ctgttgggat tgcccaatgc ggataacccg
     5401 aaataaggtc ggcaagagcg ccgccgattg atgatgcgat aattccgaaa ggtccgggaa
     5461 gaatacttgc ggcgagataa atcatagagt cgcctgcatg agagtatcca agcggtgacg
     5521 gaatcttgat aaaagcagtt gtaacggtaa caagagccgc acacattgcc gtaattgcaa
     5581 tcagctttac attatatttt gaatcggttt ttgaaacagt tttagcagtc atagtaattt
     5641 cctcacaaaa gcatttttaa atgtttttca aattcaatac cctcgttgcg gtgaataccg
     5701 agtttaacgg tttcacaaag agaggtctgt atgaatttta ccgccagatt tacggtatct
     5761 ttcaggctgt caccacggag cagtcccgaa aagacaaccg aggcaaacaa gtcacctgta
     5821 cccgaatagc ttccgcccgt cttttcacag ctgacacaaa acacctcatc atcggctgca
     5881 ataagattgt taatctttgt aacctcatcg gaaattttat gctcaatgcc tgtaacaatc
     5941 acggttttca ccttgtagag tttcaaaagc tttttggcaa gctctgcaac cgtatcgaga
     6001 aaatcgggag aatcggcatt ttcaatcagt tctgaatagt cggtatcgca cagaatacaa
     6061 cattcggtaa ggttcggggt gattacatcg gcaacagaaa cgagtttttt catctttttg
     6121 ccgagagttt cggtataaag actgtaggtt tttccgccgt caccgagaac cggatccaca
     6181 agtacaagcg tgttgtcggt ttcaaagctg tctataaatg aaagaatttt gtccacctgc
     6241 ttttcacttg agaggaaacc cgtatagatt ccgtcaggct gaaagccgag ttttttccat
     6301 tcacttgtgt aataatcaag cttatcagtg caatcgttgc agtaaaagct ttcaaatccc
     6361 gtctgacttg tcaaaacggc tgtcggcata ggacacgcct gaatgcccat tacggacaaa
     6421 acagagattg ccgccgtcag cgaacacttg ccaagccctg agagatcatt gattaataca
     6481 gctttttttg tcatcatacc acgccttaaa atttctgaac agttcaacat ttatcttgtg
     6541 atgttgtcat tatacaacac cactgtcatt atttgtatgt acaattttca gaatttttat
     6601 aggtacagtt tgtaagatgc aaaaaaagac acctcacatt gaaggtgcct tttcatattt
     6661 ataaactgat tttctgttaa aactcataat gtgaccacat agagattatt tttacgattt
     6721 tttcttcctc ataaaccata tagacaagtc tgtgctgaga atttattctg cgtgaatagc
     6781 attgtttcaa atttccgata agccgttcat aaggcggtgg attttgaaaa ggattttcgc
     6841 tgataagttc aagcaaattc tgcacattac gctttaatgc aggatactgc ttgattttct
     6901 ccttatcctt ttgggctgat tttgtaatca tcagcttata attaccactc aaattcttca
     6961 cactcctcaa gcggagtttc aattccgtcc tttaattttt cactcatacc gggaattgaa
     7021 ttaagataca ttgtttcaac aagattatta taatcttctt cacttataag cacagcattt
     7081 ccgcttttgg tgctgatgtt tataggttca ttatacttta cagtctgatc aatcaattca
     7141 aaaatttgtt ttctgaaatt agttacatta gtattaatca taatcgcacc tcctacatta
     7201 ctatatgtac attatagcgt acatataata gaaagtcaat atttttacaa caaaattccg
     7261 caataggtcg ggttggtttt cttaatcata tatcccgcct cgtgcattgt cgtggcagt
//
LOCUS       DAOMVE010000095         7264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2718_length_7264_cov_4.268831, whole genome shotgun sequence.
ACCESSION   DAOMVE010000095 DAOMVE010000000
VERSION     DAOMVE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2718_length_7264_cov_4.268831"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLS64_06315"
     CDS             <1..953
                     /locus_tag="PLS64_06315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HRL43473.1"
                     /translation="ADGSSEVSADASSEVEAGNVIKFDVKKSGWNNVKTVFCHIWKAD
                     GSGDWPAWQSKKEKCKYDASTGIATYDLSMTGNTISKSDGRVYCVIFSANTGMQSYNA
                     IMSGKCIGDTLYCTGNQLENPEDSEKKANEAKWENNSDCGPEKKITSTGNVIGSAFPE
                     GESDVTLLATYLVAYYNDDAKTSLTQKLLDELKVSPVDVMGAVTDRLNATNNEDKNKI
                     APAVEKILSGCTDPTTGKKADTAKLDSAKKTGAKAAAGSSSNGSSSSSSNGGSSSSGS
                     GSSSTGAVKSGVETTIVFVMAGLMVSAAGVMFLARKKKED"
     gene            1108..1184
                     /locus_tag="PLS64_06320"
     tRNA            1108..1184
                     /locus_tag="PLS64_06320"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1142..1144,aa:Pro,seq:ggg)
     gene            1273..2106
                     /locus_tag="PLS64_06325"
     CDS             1273..2106
                     /locus_tag="PLS64_06325"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRL43474.1"
                     /translation="MLNIGSHLSISKGFYAIGRDALSIGANTFQFFTRNPRGGSARKI
                     DIEDVNALIKLMTENNFAKILAHAPYTMNLCSAKPETREFALNTLTDDLKRMEYLPNN
                     LYNFHPGSHTGQGVKAAVEQIGTALNTAMFDDMTTTVLLETMAGKGTEVGRTFEELRM
                     IIDRVERNDKIGVCLDTCHVFDAGYDIVNNLDGVLEEFDRVIGLERLKAIHLNDSMNP
                     IGNHKDRHQKIGEGYIGIDAFGKIINNENLRNLPFFLETPNELDGYAKEISALRNLYF
                     E"
     gene            2335..2664
                     /gene="trxA"
                     /locus_tag="PLS64_06330"
     CDS             2335..2664
                     /gene="trxA"
                     /locus_tag="PLS64_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRL43475.1"
                     /translation="MIQNIRKNEFESLIKSSKPVLVDFYAEWCSPCRMLSPVIEEIGD
                     KYKAYFETVKVNVDENPELTERYGVSGIPALLLFKDGNEILRMSGVQTEERIISAISE
                     KTGESFV"
     gene            2657..3550
                     /locus_tag="PLS64_06335"
     CDS             2657..3550
                     /locus_tag="PLS64_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRL43476.1"
                     /translation="MYDLVIVGGGAAGLTAALYASRSGLDCIVLDPSSPEGSSIALTD
                     AVENYTGFSDIAGFDLIQHFYSHAKSFGANFVRQSLVGIRKNYDGFILECSDSEVKTK
                     SVILCMGATHKKLGIDGENKFEGRGVSYCAVCDGYFFKDKTVAVIGGGNTAVTEATYL
                     SHICKRVFLIHRGDALKADRVLVQKLEKSGNVKILYNTNVVKIDGDEKVKSVTLDNEK
                     ILKTDGVFVAVGLEPRTECVKGFVKTDDNGYVIADETCQTSVSGIFVAGDIRTKNLRQ
                     IITACCDGANSVYGINKFLKL"
     gene            3617..4540
                     /locus_tag="PLS64_06340"
     CDS             3617..4540
                     /locus_tag="PLS64_06340"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRL43477.1"
                     /translation="MKLLQNKKIAYLTAIFCTLLWGTAFPFIKVGYSAFEIEESDIGS
                     KLIFAGMRFMLAGFMVFLFLWISKRKPPTLSKKDILPVCSLGVVQTGLQYIFTYIGIG
                     FTSGTNTSIITACASFFTVLFVPLFFKNDRLTLMKIIGCILGFGGIIAINFGGTVSTD
                     TWFGDMMILFSTISAAAGNIVAKKVTTGRDPFPVTAFQLLTGGFILLVSGICCGGSIG
                     LINVESVSVLLWLAFVSAAAFSLWTALLKFHDASRISVFNLLVPVFGTVLSGLMLGEN
                     VFRIETLISLILISLGIAFVNMNLKKSGGKI"
     gene            4537..5187
                     /locus_tag="PLS64_06345"
     CDS             4537..5187
                     /locus_tag="PLS64_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRL43478.1"
                     /translation="MNPDIKGIISDMDGVILDTEKLYVRFWCEAALFYGYPMETHHAL
                     SIRSMARPFAIAKLKGFFGEDFDYCKVHDKRIELMDKFISENGIESKPGAAEFLGFLK
                     QNGYRVALATATAPERTKKYLSALGLYDYFDEIVCASMVAKGKPEPDIYLYAAEKLGL
                     APENCIALEDSLNGIKSASSAGCKTVMVPDLDRPSAEAIPLLYGVADNLIDVKRFL"
     gene            5270..6697
                     /locus_tag="PLS64_06350"
     CDS             5270..6697
                     /locus_tag="PLS64_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRL43479.1"
                     /translation="MWDAISAVMNSFNDFLWGWFMIILLLGTHIFLTIRTKFVQRKTF
                     KAIKLSVTKDPDSDGDISPFQALATALASTIGTGNIIGVGTAIALGGPGAVLWCWLTG
                     VFGIATKYSESLIGVKYRVKTSDGRMLGGAMYALERGFKFKTLGKILAVLFALFALLA
                     SFGIGSGVQVNAISNIMNNTFDLGTVNLFGQDASVISIIVGVLVAAITAVVIFGGIKS
                     ISRVCELLVPFMAVFYVLGCLIILGMNFDVLGKTFEMIFQDAFSLKSVAGGFLGSSLM
                     LAARYGIARGLFSNESGMGSAPIVASAAQTRNPVRQAMISSTGTFWDTVVVCLMTGLV
                     LVSSIIKNPAIDVSDGGDLTYKAFQQIPVIGTPILVIGIAAFAYSTILGWSYYGERCV
                     EYLFGRGGMIPYKLIFVFILLIGPVIKLDLVWTMADIFNALMSIPNLIAVVVLSPVVV
                     KETNYYLYGNRLDEYDKTKLPVVNK"
     gene            6754..>7264
                     /locus_tag="PLS64_06355"
     CDS             6754..>7264
                     /locus_tag="PLS64_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRL43480.1"
                     /translation="MEKSRIEKQFDFIKEIDKEKFIIRQTYLSDARKKENDAEHAWHM
                     AIMTVMLSEYANSEIDVLKTMTMLLIHDIVEIDAGDTYAYDEEAKKTQAEREMKAAKR
                     IFGLLPEDQGEKMMNLWLEFESGETAEAKFAHTMDNIQPAMLNDATDGKAWVEHIVHL
                     HSILNRNKNT"
BASE COUNT         2083 a         1455 c         1638 g         2088 t
ORIGIN      
        1 cagccgacgg ttcatcagag gtttcagctg acgcttcttc agaagttgaa gcaggtaatg
       61 ttattaagtt tgatgttaag aagtcaggtt ggaataatgt taagacagta ttctgtcaca
      121 tttggaaggc tgacggttca ggcgattggc cggcttggca gtcaaagaaa gaaaaatgta
      181 agtacgacgc ttcaacaggt atcgctacat atgatctttc aatgacaggt aacacaatct
      241 ctaagtcaga cggaagagtt tactgtgtaa tctttagtgc taacacaggt atgcagtcat
      301 acaacgctat catgagcggc aagtgcatcg gtgacacact ttattgcaca ggtaatcagc
      361 ttgaaaaccc tgaggattca gaaaagaagg caaacgaagc taaatgggaa aacaattcag
      421 actgcggtcc tgaaaagaag attacatcaa caggtaacgt catcggttct gctttccctg
      481 agggcgaatc agatgtaaca ttgctggcta catacctcgt cgcttactac aatgatgacg
      541 ctaagacaag tcttacacag aaacttcttg acgaactcaa ggttagccct gtagatgtta
      601 tgggtgctgt aacagatagg ctcaatgcta caaacaacga ggataagaat aagattgctc
      661 ctgcagttga gaagattctc tcaggttgca cagatcctac aacaggtaag aaggcagata
      721 cagctaagct tgacagcgct aagaagacag gtgctaaggc agcagccggc agctcaagca
      781 acggttcaag ttcttcttca agcaacggtg gctcaagctc atcaggttca ggttcttcaa
      841 gcacaggtgc agttaagtca ggtgttgaaa caactatcgt tttcgtaatg gcaggtctta
      901 tggtttcagc agcaggcgtt atgttccttg caagaaagaa gaaagaagac tgattttaac
      961 gaattaatct ttgattaagc atttttcgga cactgtcaat cggcagtgtc cgttttgctt
     1021 ttttaactca tggtgcaaaa atattcaaaa agtttttaaa acatcttgac aacgctcgca
     1081 gtgtttgtta aaatattaat gttgcaacgg gatgtagcgc agtttggtag cgcacacgtc
     1141 tggggggcgt ggagtcgcaa gttcaagtct tgtcatcccg accaactaaa gcctttttgt
     1201 cttttgacag aaaggctttt ttctttacga taaaccgctt actgcaaata aattaacgca
     1261 aaaggagaaa atatgcttaa tatcggctct catctttcaa tatcaaaagg tttttacgca
     1321 atcggcagag atgcactttc aatcggagca aacacatttc aattttttac acgcaaccct
     1381 cgtggcggat ctgcaagaaa aattgatatc gaagatgtca atgcacttat aaaacttatg
     1441 acggaaaata attttgcaaa aattcttgcc catgcaccat acacaatgaa cctctgttcg
     1501 gcaaaacccg aaacccgtga atttgctctg aacacgctta cggatgattt gaaacgaatg
     1561 gaatatctcc cgaataatct ttacaacttt caccccggca gtcacacggg acagggcgtt
     1621 aaagctgcgg ttgagcaaat cggcacagcg ttgaatactg caatgttcga tgatatgaca
     1681 accacggtat tgcttgaaac aatggcgggc aaaggcacgg aggtcggcag aacctttgaa
     1741 gaactgcgta tgattatcga ccgtgtggag cgaaacgata aaatcggcgt ctgccttgat
     1801 acctgccatg tttttgacgc cggttatgac attgtgaata atcttgacgg agttcttgaa
     1861 gaatttgacc gtgtaatcgg gcttgaaaga ctcaaggcaa ttcatctcaa tgacagtatg
     1921 aatcctatcg gcaaccacaa ggacagacat cagaaaatag gcgagggcta catcggaatt
     1981 gatgcgttcg gaaaaattat aaataatgaa aatttgcgaa atcttccgtt tttccttgaa
     2041 accccgaacg agcttgacgg ttatgccaaa gaaatttcgg ctttgagaaa tttgtatttt
     2101 gaatgacatt tgccttaaaa taaatcaata caaaaattgt caaaaatatt tgttgctatt
     2161 gcaatttgcg aaaattaaaa aatttaaagt cctctcgatt tttcgagggg gcttttttcg
     2221 cctttttaca ataaaatcgg tttgccgtta cacataattt gaatacgcag acaaagatat
     2281 aataattacc cttaaatcgg gaacaatagt aaaaacgatt tacggagtga tattgtgatt
     2341 caaaacatac ggaaaaatga atttgaaagt ttaataaaat catcaaagcc ggtgcttgtc
     2401 gatttttatg ccgaatggtg ttcaccgtgc agaatgcttt cgcctgtaat agaagaaatc
     2461 ggtgacaaat acaaagcata ttttgaaacc gtcaaggtca atgttgacga aaatcccgaa
     2521 ttgacagaac gctacggtgt atcgggaatt cctgcacttt tgctctttaa agacggcaac
     2581 gaaattttgc gtatgtcggg tgttcagacc gaagaacgga taatctccgc aatctccgaa
     2641 aaaacgggtg agagttttgt atgaccttgt aattgtcggc ggaggtgcgg caggacttac
     2701 ggcggcactc tatgcctctc ggagcggact tgactgcatt gtgcttgatc cgtcatcacc
     2761 cgagggcagt tcaattgccc tcacggatgc ggttgaaaac tacacgggct tcagcgatat
     2821 tgcaggcttt gacctcattc agcactttta ttcacacgca aagtcattcg gtgcaaattt
     2881 tgtcagacaa tcccttgtcg gcataagaaa aaattatgac ggttttatcc ttgaatgttc
     2941 cgacagcgag gtcaagacaa aatctgttat tctctgcatg ggcgcaaccc acaaaaaatt
     3001 gggaattgac ggcgaaaata agtttgaggg cagaggagta agctattgtg ccgtgtgtga
     3061 cggctatttt ttcaaagaca aaacagtggc ggtaatcggc ggaggaaaca ccgccgttac
     3121 cgaggcgacc tatctttctc atatatgcaa acgggttttt cttattcaca ggggcgacgc
     3181 attaaaagcc gacagagtgc ttgtgcaaaa gcttgaaaaa tcgggcaatg tcaaaattct
     3241 ttacaacacc aatgttgtga aaattgacgg tgacgaaaag gttaaatcgg ttacgcttga
     3301 caacgagaaa attctgaaaa ctgacggagt ttttgttgcg gtaggtcttg aaccgagaac
     3361 cgaatgtgtc aagggttttg taaaaactga tgacaacgga tatgtgattg ccgatgaaac
     3421 ctgtcagaca tccgtgtcgg gaatttttgt tgcaggggat atccgcacaa aaaatctgcg
     3481 acaaattata accgcctgtt gtgacggtgc aaattctgtt tacggaataa acaaattttt
     3541 gaaattgtaa ggttttcttt ttctgatttt tatggtatac tatatttgat attgttaatt
     3601 tttccggatg aataaaatga aactgttaca aaataaaaaa atagcatatt taacagcaat
     3661 tttctgcaca cttttgtggg gaacggcttt tccgtttata aaggtcggct actctgcttt
     3721 tgagattgag gagtcggata tcggctcaaa gctgattttt gcaggaatgc gctttatgtt
     3781 ggcgggattt atggtgtttc tctttttgtg gatatcaaaa agaaaaccgc cgacgctttc
     3841 aaaaaaagat atactgcccg tctgttcact cggagtggtg cagacaggat tgcagtatat
     3901 ttttacatac atcggaatcg gttttacaag cggaacgaac acctctataa taacggcttg
     3961 tgcgtcgttc tttaccgtac ttttcgtgcc gttgtttttc aaaaacgaca ggctcacgct
     4021 gatgaaaatt atcggttgta ttctcggatt cggcggaatt atcgcaatca atttcggcgg
     4081 aactgtcagc acggacactt ggttcggcga tatgatgatt ctcttttcaa caatatctgc
     4141 ggcggcaggc aacatcgttg cgaaaaaagt cacaacagga cgagatccgt tccctgtaac
     4201 cgcttttcag cttttaacag gcggttttat attgcttgtg tcgggcatat gctgcggtgg
     4261 cagtattggt ttgataaatg ttgaaagtgt ttccgtgttg ctctggcttg cgttcgtatc
     4321 agcggcggca ttttcgctgt ggacagcact tcttaaattt catgatgcaa gcagaatttc
     4381 cgttttcaac ctgcttgttc ccgtgttcgg aacagttttg tcggggctta tgctcggtga
     4441 aaatgttttc agaatcgaaa cgctcatctc gcttattctg atttcactcg gaattgcgtt
     4501 tgtaaatatg aatctaaaaa aatcgggagg taaaatatga atcctgatat aaaaggtatt
     4561 atttccgata tggacggtgt cattcttgac acagaaaagc tgtatgtccg tttttggtgt
     4621 gaggcggctc ttttttacgg ctatcctatg gaaacgcacc acgcactcag tattcgttca
     4681 atggctcgcc cgtttgcaat tgcaaaatta aagggctttt tcggggagga ttttgactac
     4741 tgcaaggttc acgataaaag aattgagctt atggataagt tcatctcgga aaacggcatt
     4801 gaatcaaagc ccggtgccgc tgaatttctc ggctttttaa agcagaacgg ctacagggtt
     4861 gcccttgcaa ccgcaaccgc tccggagaga acgaaaaaat atctttcggc tctcggtctt
     4921 tacgattatt ttgacgaaat agtttgtgcg tcaatggttg caaaaggaaa gcccgaaccc
     4981 gacatttatc tttatgccgc agaaaaactc ggacttgctc ccgaaaactg cattgcactt
     5041 gaggactcgc tcaacggcat aaagtcggca agctcggcag ggtgtaaaac ggttatggtt
     5101 cccgaccttg accgtccgag tgcagaggca attccccttc tttacggagt tgcagacaac
     5161 cttattgatg taaaacgatt tttgtaaaaa atattaacgg acataaagca ggttttgctt
     5221 ttgtatatta aacagggtag gtttatacta aaacaaggag gcgtacaata tgtgggatgc
     5281 gatttcagct gtgatgaata gcttcaatga cttcctgtgg ggttggttta tgattatcct
     5341 tcttctcgga actcacattt tcctcacaat tcgcacaaaa tttgtacaga gaaaaacttt
     5401 taaggcaatc aaactttcgg tgacaaagga tcccgattca gacggtgaca tcagcccgtt
     5461 tcaggctctt gcaacagcac ttgcctcaac aatcggcacg ggtaatatca taggcgtagg
     5521 tactgcaatt gcactcggag gccccggtgc ggttctctgg tgctggctca ccggtgtttt
     5581 cggtatcgca acgaagtatt ccgagtcact catcggtgta aagtatcgtg taaagacctc
     5641 tgacggcaga atgctcggcg gcgctatgta tgcccttgaa agaggattta agtttaaaac
     5701 tctcggcaaa attttagccg ttctttttgc actttttgca ttgcttgcat ccttcggcat
     5761 cggttcgggt gttcaggtta atgcaatttc aaacattatg aacaacacct ttgaccttgg
     5821 tacggtgaat cttttcggtc aggacgcttc ggttatttcg attattgtag gtgttcttgt
     5881 tgccgcaatt acagccgttg taattttcgg aggtatcaag tcaatctcaa gagtttgcga
     5941 actccttgta ccgtttatgg ctgttttcta tgttctcggc tgtcttatta tcctcggcat
     6001 gaactttgat gttctcggta agacatttga aatgattttt caggacgcat tttctctcaa
     6061 gtccgttgca ggcggtttcc tcggttcaag ccttatgctt gccgctcgtt acggtattgc
     6121 aagaggcctt ttcagtaacg aatccggtat gggttctgct ccgatagttg cctctgccgc
     6181 tcaaacaaga aaccctgttc gtcaggctat gatttcttca acgggtactt tttgggacac
     6241 agtcgttgtt tgtcttatga caggtcttgt gcttgtaagc tcaattatca aaaatcctgc
     6301 aattgatgtt tccgacggcg gcgaccttac atacaaagcc ttccagcaga ttcccgtaat
     6361 cggcacaccg attcttgtta tcggtattgc cgcttttgca tattcaacaa ttctcggctg
     6421 gtcatactac ggtgaacgct gcgttgaata tcttttcggc agaggcggta tgattcctta
     6481 caagctgatt tttgtattta ttcttctcat cggtcctgtt atcaagctcg accttgtttg
     6541 gacaatggct gacatcttca atgcacttat gtcaattccc aaccttattg ccgtagttgt
     6601 gctttcgcct gttgttgtaa aagaaacgaa ctattacctt tacggcaaca gactggatga
     6661 atacgataaa accaaacttc ctgtagtaaa taaataatca cataaagcct gcactatgcc
     6721 aatgtgcagg cctgaatttt aagagggaac attatggaga aatcaagaat tgaaaagcaa
     6781 tttgatttta taaaggaaat tgataaggaa aaattcatca tccgtcaaac ctacctttcc
     6841 gacgctcgaa aaaaggaaaa cgatgccgag cacgcatggc atatggcgat aatgaccgtt
     6901 atgctttccg aatatgcaaa ttcggaaatt gatgtgctta aaacaatgac aatgctcctg
     6961 attcacgata ttgtcgagat tgacgcaggc gatacatatg cctatgatga ggaagctaag
     7021 aaaactcagg cagagcgtga aatgaaagcc gcaaaaagaa ttttcggact tcttccggaa
     7081 gatcagggcg aaaagatgat gaatctgtgg cttgaatttg aaagcggtga aacagccgaa
     7141 gcaaagtttg ctcacacaat ggataacatt cagcctgcca tgctcaatga tgcaactgat
     7201 ggcaaggctt gggtagagca tattgttcac ctgcacagta tccttaaccg caacaaaaat
     7261 actg
//
LOCUS       DAOMVE010000096         7173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2772_length_7173_cov_3.708907, whole genome shotgun sequence.
ACCESSION   DAOMVE010000096 DAOMVE010000000
VERSION     DAOMVE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2772_length_7173_cov_3.708907"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLS64_06360"
     CDS             complement(<1..285)
                     /locus_tag="PLS64_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRL43481.1"
                     /translation="MVEVKNLTKFYGSIKAVDDISFTVETGEVLGFLGPNGAGKSTTM
                     NMITGYLSSTSGTVTIDGCEILDDPIHAKSKIGYLPEIPPLYIDMTVKKYL"
     gene            533..886
                     /gene="spoVAE"
                     /locus_tag="PLS64_06365"
     CDS             533..886
                     /gene="spoVAE"
                     /locus_tag="PLS64_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HRL43482.1"
                     /translation="MQYVNAFWVGGLICVAGQILIDKTKLTPARILTGFVVAGVILGA
                     IGLYKPLVDFAGAGASVPISGFGFLMSEGVRKALSQDGALGILTGGLTSAAAGISAAI
                     VFGLIAALCTKSGDK"
     gene            complement(1056..1970)
                     /locus_tag="PLS64_06370"
     CDS             complement(1056..1970)
                     /locus_tag="PLS64_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HRL43483.1"
                     /translation="MKKILAILLVGVMSVTAFGFNASAVEKTDAEIQQEKLGYILNLN
                     KDIWLDCAVIYDGVWPSIMDNAREVYDTDGLSADEYTVALKQVYDMYYDAPLVNIEYA
                     EATYKNALKEENYNNWYSENEWNDFQEKLKNLGDAFDYYYAHKNEFPCNQLTKAFKEM
                     MTSYNKMTNAYTVKGDVNGDGEVNIEDATLVQKYVAHIENLTGAQKMLTNEKNYENIT
                     VTTATNLQKHIVGLEPEFKNNDIFIDEQSCVSSLDDTLRWYNYCNFNICPRRNDVTSG
                     TYIDNNFICMDKIFGYRIWCDKNGYDYL"
     gene            complement(2324..5062)
                     /locus_tag="PLS64_06375"
     CDS             complement(2324..5062)
                     /locus_tag="PLS64_06375"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRL43484.1"
                     /translation="MPAVKKRKKAEPVTLNNKSNSKGFFSKNMFIVLAFVIPFVLMTA
                     AFGVMKVSPFGDNQILVTDLWHQYYPFLADMQEKLKHGESLFWSWTQGGGVNYFALMS
                     YYLMSPLNFLTVFLPSEWLREFLMFSVVIKVACASMFMAIFLRSLFKKNDFSLVIFGC
                     SFGFCAFFMGYYWNTIWLDTVCITPLVALGTVKLFTEGKFRLYIVSLALSLLTNYYIG
                     LFACIFVLLSFIAYNIVKWDGIKKFATNLLRTGIYSLIAIGLTAFFLLPAFFGLQNTN
                     ASGATFPTTFAINIGSTNDLMGVLEAIRKILSNFITFAAPAIKEADALPNIACGTLSL
                     VLGILFFTSKKISLKEKIVDGCLIGFMIISCIIRQLDYIWHGFHFTNMIPYRFSYLIS
                     FVLVVMAFRAFMLLESSSCWDVILAALFVALVIIFGIGTQETYALVGTAVIAAVICVL
                     LFLYTKRIVPKQVLLIVFGVIIIGESAAAGYIGVKTTTVTGTYDYPRGEENTAQVIDY
                     MDSLESNTTEMWRAEMTSTQTLNDAALNHYNGLSMFNSMANVDMTVLFENFGMMGWKS
                     GNRYTYAEGSPVTNLFMNLKYLIARDNIYMNTYDLTEVYGVGNVKLLQNNHYLPMGFM
                     TNSALASWQVDENEDQFNPFDKQNEFFKLATGIKDDVYTPLDVVSQGHTDYNQFPVNK
                     TGYGRYSFSCTDTTVTPHVKWNYEAPKDGLYLMYADISGGDDVTVMINDVAQSKTYGM
                     GRSYIACIGQCKKGDKISVYSNLQQGQSGSAMVFVDVLNQDVFEEGYNKLSKSVMTTT
                     KLTGSSMEGTINAQENGLFYTSVPYEEGWKAYVDGKEVTITPVGNALVAFNLDKGEHT
                     IKLEYYPKGFAIGLTVTIICAATFAFLCVWTYIIKKRRKKKGDISENPEEVQINAE"
     gene            5501..6475
                     /locus_tag="PLS64_06380"
     CDS             5501..6475
                     /locus_tag="PLS64_06380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRL43485.1"
                     /translation="MKTIDLVVPCYNEEAGLEHFVLETNKVINSLPEYSFRYILVNDG
                     SKDRTYLIMKKLAKTFNNIKYISFSRNFGKEAAMYAGLKHTTADYVVVMDADLQHPPQ
                     LFPQMIQAVEHEGYDCCATKRADREGESKFRGIFSKLFFKLSNRLTDVKMPYGAMDFR
                     MMSRQMVDSILELSEVQRFSKGIFSWVGFNTKWIAYKTVDRQLGQTTWKFSSLFKYAI
                     EGITSFSVAPLRFTAVMGFIIFVISLIYIIITLIQTLFLGITTPGYVTTLCAILFMGG
                     ITEMSIGILGEYIGKIYMEAKDRPIYITKFSNFEDEDNEEKDDKEING"
     gene            6475..6933
                     /locus_tag="PLS64_06385"
     CDS             6475..6933
                     /locus_tag="PLS64_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HRL43486.1"
                     /translation="MGNFIKQFFDIKFWKFILVGILNTVVGMGLQFLFFNLCGWNEWV
                     SSLIGYILGSILSYFLNKYFTFKNKDKGWKPIVKFALNIAICYGLAYGIAIPLISMLC
                     TANSWTMFGWSVEKFAGNFSMIVGACLFVAFNYIGQRFFAFKEKKEENNK"
BASE COUNT         2179 a         1459 c         1359 g         2176 t
ORIGIN      
        1 aagatatttt ttaaccgtca tatcaatgta caaaggcgga atttcgggaa gataaccgat
       61 ttttgacttt gcgtgaatcg gatcgtcaag aatttcgcat ccgtcaatgg taacagtacc
      121 gcttgttgat gaaaggtaac ctgttatcat attcattgtt gtggatttgc ccgcaccgtt
      181 aggtccgaga aatcccagaa cttcacctgt ttcaacagtg aagctgatgt catcaacggc
      241 ctttatgctg ccataaaatt ttgtaaggtt tttaacctcg accatgtgac ttttcactcc
      301 ttaaaattaa ttgcgtaatt tctgtgattt gaatgtgccg tacaaaaaca cagaaacgaa
      361 cattattgat tatactataa aacgagcagt ttgtgtagag tttttccaaa taaatcaacg
      421 caaactcaca aaaattcagt ttttcatcac ataaagaaat gtcttgggga attattcacc
      481 aaaaactcgg aacaatattt attgataatg acaaaacaaa ggaagtgtca gtatgcaata
      541 tgtaaatgca ttctgggttg gcggactgat ctgcgtggca ggtcagattc tcattgacaa
      601 aacaaaactc actcccgcaa gaatacttac gggctttgtt gtcgcaggcg taattctcgg
      661 ggcaatcgga ctgtacaaac cgcttgttga ttttgcggga gccggcgcaa gtgtgccgat
      721 cagcggattt ggttttctta tgagcgaggg cgtgcgaaag gcgctctctc aggacggcgc
      781 actcggaatt cttacaggag gtcttacaag cgccgccgca ggaatctccg ccgcaatagt
      841 tttcggactt atcgccgccc tgtgtacaaa atcgggagat aagtaaaggc ggtatgatat
      901 agaaaattat tgcacccaag catagtattg taatcgtttg tgcaatcaac tgtaggtgcg
      961 ttcatcggat gactgcgcct actgattgtt ttacaacaaa aaccgccgta cagatttttc
     1021 gctgtacggc ggtttaattt attcacctta ttcagttaga ggtaatcata tccatttttg
     1081 tcacaccata ttctgtatcc aaaaatttta tccatgcaaa taaaattatt atctatgtat
     1141 gtgccacttg ttacatcatt tctgcgagga cagatattga aattgcagta attataccaa
     1201 cgcagagtgt cgtcaagtga acttacacaa ctctgttcgt caatgaaaat atcattgttt
     1261 ttaaattcag gctcaagacc tacgatatgt ttctgaaggt ttgttgcagt cgtgaccgta
     1321 atgttttcat aatttttttc atttgtcagc attttctgcg cacctgtaag gttctcaata
     1381 tgagcaacat atttttgaac aagcgttgca tcttcaatat ttacttcacc gtcaccgttc
     1441 acatcacctt tgacggtata cgcattggtc attttgttgt aagatgtcat catctcttta
     1501 aatgcttttg taagttgatt acacggaaat tcattcttat gtgcataata ataatcaaat
     1561 gcgtcaccaa gattttttaa cttttcctga aaatcattcc attcattttc tgaataccaa
     1621 ttattataat tttcttcttt caaagcattt ttatatgtag cctctgcata ttcaatatta
     1681 acaagcggtg catcgtaata catatcataa acctgtttta gtgcaacagt atattcatct
     1741 gccgataaac cgtcagtatc atacacttct cttgcgttgt ccattattga cggccaaact
     1801 ccatcatata tcactgcaca atccaaccaa atatctttat tcaaattcaa tatgtaacct
     1861 aacttttcct gctgaatttc agcgtctgtt ttttcaacag cagatgcatt aaagccaaat
     1921 gctgtaacag acataactcc tacaagtaat attgctaata tctttttcat tagttagtcc
     1981 cccttgttat atttaatgcc attttctgaa tcaatgttgc atcaattatg tcaatatcac
     2041 catttctatc aacatcaccc aaaatgacga tcctaaactt agttaagtta attagaacat
     2101 atcgttaatt taattaattt tatttttatt gcaattattg caatttaaaa tagaacttta
     2161 taactaacat tattatagca taattaaata agtattgata gttttttatt atttatgtta
     2221 tagaatttca ttatgatttt ttgtgcattt tgtcaaatta aacaaaaacc gccgtacaga
     2281 tttttcgctg tacggcggta atgtttaaaa atatttaatt ttattattca gcgttaatct
     2341 gaacttcttc gggattttca gaaatatcgc ctttcttttt tctgcgtttt ttgatgatat
     2401 atgtccatac acagaggaac gcaaaggttg cggcacatat gattgtcacg gtcaagccta
     2461 ttgcaaatcc ctttggataa tactcaagct tgattgtatg ctcgcccttg tcaagattga
     2521 atgcaacaag tgcgtttccg acaggagtaa ttgttacttc cttgccgtca acatatgcct
     2581 tccagccttc ttcatacgga acagaggtgt agaacaatcc gttttcctgt gcattgattg
     2641 ttccctccat agaactgcct gtaagctttg ttgttgtcat tacgctcttt gaaagcttat
     2701 tgtaaccttc ttcaaagaca tcctgattca gaacatcaac aaataccatt gcagaacccg
     2761 actgtccctg ctggagattt gaataaacgg aaatcttgtc gccctttttg cattgaccga
     2821 tgcaggcaat gtagcttctg cccataccgt aggtcttgct ctgagcaaca tcgttaatca
     2881 taacggtaac atcgtcaccg cccgaaatat ccgcatacat aagatacaag ccgtccttag
     2941 gtgcttcgta attccacttt acatgcggag taactgttgt atcggtacag ctgaatgaat
     3001 atcgtccgta acctgtctta tttacgggga actgattgta gtctgtgtgt ccctgtgaaa
     3061 caacatcaag cggagtgtaa acatcatcct taatgcctgt tgcaagtttg aaaaattcat
     3121 tctgtttgtc aaacggattg aactgatctt cgttttcatc aacctgccag cttgcaagtg
     3181 cggagtttgt cataaagccc atcggcagat agtgattgtt ctgcaaaagc tttacattac
     3241 cgacaccgta aacctctgta aggtcatagg tgttcatata gatattgtca cgggcaataa
     3301 gatatttaag attcataaag aggtttgtaa ccggtgaacc ctcggcatag gtgtagcggt
     3361 taccgctctt ccaacccatc ataccaaagt tttcaaacag cacggtcata tcaacattag
     3421 ccattgagtt gaacatcgaa agtccgttgt agtggttgag agccgcatcg ttaagcgtct
     3481 gagttgatgt catctccgct ctccacattt cggttgtgtt gctttcaagg ctgtccatat
     3541 agtcaattac ctgtgcagta ttctcctcgc ctctcggata atcgtaagta cccgttacag
     3601 ttgttgtctt aacaccgata taacctgcgg cggcactctc gcctattatg attacgccga
     3661 atacaatcaa aagaacctgt ttcggaacaa ttctctttgt atagaggaaa agcagaacgc
     3721 agattacagc cgcaataact gccgtgccga caagcgcata agtctcctgt gtgccgatac
     3781 cgaagatgat aacaagcgca acaaaaagtg cggcaagaat aacatcccag caagagcttg
     3841 attcaaggag cataaaagct ctgaatgcca taacaacaag tacaaagctg ataagatagc
     3901 tgaaacggta cggaatcata tttgtaaagt ggaaaccgtg ccagatgtag tcaagctgtc
     3961 tgatgataca gctgataatc ataaaaccga taaggcagcc gtcaacgatt ttctctttga
     4021 gcgaaatttt cttgcttgtg aagaaaagaa ttccgagtac aagagaaagt gtaccgcagg
     4081 caatgttagg caatgcgtca gcctctttaa ttgcaggagc ggcaaatgtt atgaaattgg
     4141 aaagaatctt tctgattgcc tcaagcacac ccataaggtc atttgtcgag cctatattga
     4201 ttgcaaaggt tgtcgggaaa gttgcccccg atgcgtttgt gttttgcaga ccgaagaatg
     4261 ccggcaacag gaaaaacgca gtaagtccga ttgcaatcaa tgagtagatg cctgtgcgaa
     4321 gaagatttgt tgcaaacttt ttgatgccgt cccattttac aatgttgtat gcaataaatg
     4381 acaacagtac aaaaatgcag gcgaacaaac ctatataata gttagtcaaa agtgaaagtg
     4441 caagcgaaac tatgtaaagt ctgaacttgc cctctgtgaa gagctttact gtgccaagag
     4501 caacaagcgg tgtgatacaa accgtatcaa gccagattgt gttccaatag tagcccataa
     4561 agaatgcaca aaagccgaaa ctgcatccga aaatcacaag agaaaaatca ttcttcttaa
     4621 aaagactgcg taagaagatt gccataaaca tacttgcaca tgcaaccttg attacaacag
     4681 agaacatcaa aaattctctc agccattcac tcggcaaaaa taccgtaaga aaattcagcg
     4741 ggctcataag gtagtatgac ataagtgcaa agtagtttac acctccgccc tgcgtccatg
     4801 accagaacag cgattcgccg tgtttaagtt tttcctgcat atccgcaagg aacggataat
     4861 actgatgcca caggtcggtt acgagaatct gattgtcacc gaacggcgaa accttcataa
     4921 ctccgaaagc ggctgtcata agcacaaacg gtatgacaaa agccaaaacg ataaacatgt
     4981 tttttgagaa aaagcctttg ctgttgctct tgttattcag cgtaacaggc tcagccttct
     5041 tccttttctt tacagcgggc ataatttgac ctccgaaata ttttgtaatt gttttccgat
     5101 atgcacaaaa atgcaatcgg gtacaagtaa tccattctat tttaacgctt ttcgtgtgtg
     5161 atgtaaataa aaaaacataa aaatcttaaa aaaaatagac ttttggcgtt agctttgtta
     5221 taatctaagt gcggataatc tccacctctt tgaggttgga gattatcttt ggaatttagt
     5281 gtgagtttca ctccctctaa attccacgga gctttcgctc cttcgcactg attgcaatct
     5341 gtcgcaagct ctgctccttg cgagcaaagc attgcttgct ccgatttaaa tctaagtgaa
     5401 actatttaca gacaaagcag attatcattc agataaaaaa caagtgctac tgttctcacc
     5461 cctatgcggt gagtttaact ttataaaggt gatgtaaaaa atgaaaacca tagacttagt
     5521 tgttccctgc tataatgaag aagctggact tgagcatttt gtgcttgaaa ccaacaaggt
     5581 tatcaactct ctgcccgagt attctttcag atacatactt gtaaatgacg gcagtaagga
     5641 caggacctat ctcattatga aaaagcttgc gaaaactttt aacaatatca agtatatttc
     5701 gttctcacga aattttggca aagaggcggc aatgtatgcc ggactcaagc atacaacggc
     5761 tgactatgtt gttgttatgg acgccgacct tcagcatcct ccgcagttgt ttcctcagat
     5821 gattcaggct gttgaacatg agggttacga ctgctgtgcg acaaagcgtg ccgaccgtga
     5881 gggtgaaagc aagttcagag gaattttttc aaaactgttc ttcaagcttt caaataggct
     5941 tacagatgta aaaatgccct acggcgcaat ggacttccgt atgatgagcc gtcagatggt
     6001 tgactcaatc cttgaactca gcgaagttca aagattttca aagggcattt tctcatgggt
     6061 tggcttcaat acaaaatgga ttgcctacaa aacagttgac cgtcagctcg gtcagacaac
     6121 atggaaattc tcaagtctgt tcaaatatgc aattgaggga atcacaagct tttcggttgc
     6181 accgctcaga ttcacggctg ttatgggatt tatcatcttc gtaatttcgc tcatatacat
     6241 tataataacg ctgattcaaa cgctttttct cggaattacc acacccggtt atgtcaccac
     6301 gctgtgcgca atcctcttta tgggcggaat caccgaaatg tcaatcggta ttcttggcga
     6361 atacatagga aaaatttata tggaagccaa ggacagacct atttacatta caaaattctc
     6421 caattttgaa gatgaagata acgaagaaaa agatgataag gagattaacg gttaatgggt
     6481 aattttataa agcagttttt tgacattaaa ttttggaagt ttatacttgt cggaatttta
     6541 aacacggttg tgggtatggg acttcaattt ctgttcttta acctctgtgg ttggaatgaa
     6601 tgggtttcat cgcttatcgg ctacatcctc ggcagtattt taagctattt tctcaacaaa
     6661 tacttcacat tcaaaaacaa ggacaagggc tggaaaccaa tcgttaaatt tgcactcaat
     6721 attgcaatat gctacggact tgcctacgga attgcaattc cgctcatcag tatgctctgt
     6781 acggcaaaca gctggacaat gttcggctgg agtgttgaaa agtttgcagg aaacttctca
     6841 atgatagtag gcgcttgcct atttgttgca ttcaactaca tcggacagcg tttcttcgct
     6901 ttcaaagaaa aaaaggaaga aaacaataag taatccaacc cattaaaagt tttcaacatt
     6961 taataaacac caaataaaca ccaaaaggac tgaaagcgat tatctttcag tcctttttat
     7021 atatacggca aaccgccgtt ccccattcgt gaagatagaa tgttaatcat ctatgcaaaa
     7081 taaatacaca agtttaatta cgccaaatta aatatgtata aacaaagaat ttgattgtgg
     7141 cgggcgaata atatcctccc tacgaaattt gtt
//
LOCUS       DAOMVE010000097         7143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2783_length_7143_cov_2.728414, whole genome shotgun sequence.
ACCESSION   DAOMVE010000097 DAOMVE010000000
VERSION     DAOMVE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7143
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_7143_cov_2.728414"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    236..245
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(483..559)
                     /locus_tag="PLS64_06390"
     tRNA            complement(483..559)
                     /locus_tag="PLS64_06390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(523..525),aa:Arg,seq:cct)
     gene            722..810
                     /locus_tag="PLS64_06395"
     tRNA            722..810
                     /locus_tag="PLS64_06395"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:756..758,aa:Ser,seq:tga)
     gene            842..931
                     /locus_tag="PLS64_06400"
     tRNA            842..931
                     /locus_tag="PLS64_06400"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:875..877,aa:Ser,seq:gct)
     gene            1158..1310
                     /locus_tag="PLS64_06405"
     CDS             1158..1310
                     /locus_tag="PLS64_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43487.1"
                     /translation="MAESKLRELSIDLYNRCGTIRRILVASINTAKENLQKEKINSKS
                     KPNISV"
     gene            1415..2215
                     /locus_tag="PLS64_06410"
     CDS             1415..2215
                     /locus_tag="PLS64_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL43488.1"
                     /translation="MGFLLGKTAVITGGGRAVLSDGGCGSIGYGIAVAYAKEGANLVI
                     TGRNVKKLEDAKEELERLYGVNVLPVQADVSKDGDNEKVVRNVVDRAVETFGGIDVLI
                     NNAQASASGITLADHTTEQFDLAVYSGLYATFYYMKACYPYLKKSKGTVINFASGAGL
                     FGNFGQCAYAAAKEGIRGLTRVAANEWGKDGINVNIVCPLAWTAQLEKFEQAYPDAFK
                     ANVKVPPMGHFGDVEKEIGRACVALASPDFKYMSGETITLEGGMGLRP"
     gene            2362..2532
                     /locus_tag="PLS64_06415"
     CDS             2362..2532
                     /locus_tag="PLS64_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43489.1"
                     /translation="MGIFDIKNTDIFSKDYDSANAWLEYNIFNECTKDENGESFDESD
                     ENNFWDYLKKNK"
     gene            2660..3535
                     /gene="dpsA"
                     /locus_tag="PLS64_06420"
     CDS             2660..3535
                     /gene="dpsA"
                     /locus_tag="PLS64_06420"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HRL43490.1"
                     /translation="MKKSDIRTFGIIGGDKRQLFLAKSISDSCYDVLLGGFDSLESYG
                     SLSLCNVKKAISESDALIFPLPSIRTDGSLNTPFSNENILLDGDDIEIILKKPVFTTM
                     KSRFLKAYPRLSDGEIFDYGARDDFAILNALPTAEGAIECAMREYEGTISGSKCLVTG
                     FGRIGKILAHKLVLLGANVTVSARKPSDLAYVKALGYNALNTENLRTVKRFDIVFNTI
                     PRLIFDRELLMNTDTNTLIIDLASLPGGVDFDTAEKLGIYAVRALSLPGKCAPKTAGE
                     IIKTTVFDIIKEVYR"
     gene            3532..4104
                     /locus_tag="PLS64_06425"
     CDS             3532..4104
                     /locus_tag="PLS64_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HRL43491.1"
                     /translation="MTKATIGFAMCGSFCTFSKAFTQMQRLVDSGYDVIPIMSQNAFS
                     TSTRFGKAEETVKRAEEITGKAVLHSVVQTEPIGPKKMCDLLVIAPCTGNTLAKLSLG
                     ITDTAVTMAAKSHLRILRPVLLCVATNDALGASAQNIGRLLNTKNIYFVPLKQDDCIK
                     KPNSLVADFDKLEDCVTLALQGKQHQPIFL"
     gene            4224..5873
                     /locus_tag="PLS64_06430"
     CDS             4224..5873
                     /locus_tag="PLS64_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRL43492.1"
                     /translation="MRKRVNKIISVALSATLAFGVFTALPMSANAVVSTASEVSAEAI
                     PKHELYKSYTYGGYKYRIVGQKSNGRFNAWIESYSGKSASVNVPASVGDCDMVGIDNN
                     CFSFNKTLKTIIVPKGIAEIGSSAFLGCTALTSVSLPSTLTKINFWAFKNCTSLSTLS
                     IPSSVVSIGDNAFDNCLWYTNQSNGIVYAGKVAYKYKGKLADNSSVTIKSGTVSIADY
                     AFTDQKLTSVTLPSSLVSIGEQAFSYTNLKTVTIPKSVSSMGYNPFAYCSQLSSITVQ
                     SGNTNFYVQNDLLIAKNHMYSVTKDITDPDAPSTESRSYTSYAPYGSVVISFPSASTL
                     KTVTIPETVKAIGNYAFAGSKIEKLTLNSGLESILTSAFSGCTNLSSVSFSDSIISIC
                     DSSFEECTSLKNLKFGKNLEFISYYAFYNCQNLQSVTIGENVKAICCDSFGNCNALVI
                     NGKIGSTAETFAKKYGYKFNSSETTRLKGDVDNNGIINVVDATDIQKYVVNLTDENGN
                     KFIDVNNAEDVYVADVNGDGIINVVDATLIQKYIVRLVESL"
     gene            complement(5938..6147)
                     /locus_tag="PLS64_06435"
     CDS             complement(5938..6147)
                     /locus_tag="PLS64_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL43493.1"
                     /translation="MKLRIKDLREDNDLTQKEIAKVLMCDQSLYSKYERGEREIPLSL
                     LIKLADCYGTSLDYLTCRTDNPNIK"
     gene            complement(6497..6721)
                     /locus_tag="PLS64_06440"
     CDS             complement(6497..6721)
                     /locus_tag="PLS64_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRL43494.1"
                     /translation="MYKRIRELREDADLTQKEVSKILHCSQQVYSNYELGQRDIPTVV
                     LICLAKHYHTSTDYILGLTDNKDLPNYTHN"
     gene            6907..>7143
                     /locus_tag="PLS64_06445"
     CDS             6907..>7143
                     /locus_tag="PLS64_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43495.1"
                     /translation="MRKHSKFITILLSALLAVCLMQTVFVQAFATEMGDDDFGWGNAS
                     TSDEAETVYPVSMEVVKAPESVTVNNGRITDSTFW"
BASE COUNT         2217 a         1279 c         1522 g         2115 t
ORIGIN      
        1 tcggtctcca agttgtttta ttttggtgac aagaaatccg ccattcattt ccatataaaa
       61 gctcctatat agtagtttat taaattatac tatataggag ctttattgtc aagagcttgt
      121 atgtctgaat agaggtaaat ttcgatttgt cgcttcgttt atgccacaaa gaatggattg
      181 ctgatcataa ataataaaaa ctcactcggc ttaagtgctt tttctaaatg caaatnnnnn
      241 nnnnntacta ttccggactg acggacgccg ccattgtaga ataaatcact ttgcagtaac
      301 tgttacgccc gcaaccttaa tcaaagatca aaattttttc taatcatttt cctgttagct
      361 ataaaaaagc acaacgccac agagttttaa aactctgtgg cgttgtgctg ctctcaatcg
      421 gtcacttttt gaaagtatcc ttatgtgagc ctgccttttg ctgggttttc cggcggagtt
      481 tttggcgtgc caaaagggat tcgaaccccc gacctttcgc ttaggaggcg aacgctctat
      541 ccaactgagc tattggcaca taacataact attgtatatg atggcttgcg atttgtcaag
      601 aaattgggaa attttcttat tttactgaat attcaaaaaa attaaaaata agtgttgaca
      661 aatggagaaa cttgtgttag aataaatctt gctgaaatgc tgaaaggcaa gatgagcata
      721 cggagaggtg tccgagtggt ttaaggagct agtcttgaaa actagtgatc ccgcgaggga
      781 ccaagggttc gaatcccttc ctctccgcca ttttatttta tagtgaattt aaacttcaca
      841 cggaggatta ctcaagtggt gaagaggctc ccctgctaag ggagtaggtc gctaacgcgg
      901 cgcgagggtt caaatccctt gtcctccgcc aagaaaaaag cacttgcttt tgcaagtgct
      961 tttttcagtg aaataaatcc aaaaggattt gtgaaatacc tatcggtgtg aaatatggat
     1021 tttccatgtg aagtgcctga cggcgtgaaa ggatttattt tatttcactt tctgcgcaag
     1081 cagaaaattt cacaatacaa tagtattatt tcacatacga agtatatttc acttgttata
     1141 aattaaaaga ggttcgtatg gctgaatcaa agcttagaga actatcaatt gatttatata
     1201 accggtgcgg cactattcgc agaatattgg ttgcatctat taatactgca aaagaaaatt
     1261 tacaaaagga aaagattaat tcaaaatcaa aacccaatat ttccgtataa tagcattacc
     1321 ccgattgaca ccaaacttaa taactggtaa tataaggctg tgagaatatt tgtattcttg
     1381 cagcatattt ttttaccaaa aggaggatgg attaatggga tttttattag gaaagaccgc
     1441 tgttattaca ggcggaggaa gggctgttct cagcgacgga ggttgcggtt caatcggcta
     1501 cggcattgct gttgcttatg caaaagaggg tgcaaacctt gttattacag gcagaaatgt
     1561 caaaaagctt gaggatgcca aagaagagct cgaaagactt tacggagtaa atgtgttgcc
     1621 tgttcaggct gatgtcagca aggacggcga taacgaaaag gttgttcgga atgttgttga
     1681 cagagctgtt gaaacattcg gcggaattga tgtcttgatt aacaacgctc aggcttctgc
     1741 gtcgggtatt acgcttgctg atcatacaac agagcaattt gaccttgctg tttattcggg
     1801 attgtacgca accttctatt atatgaaggc ttgctatccg taccttaaaa aatcgaaggg
     1861 aacggttata aactttgctt cgggtgccgg acttttcgga aacttcggtc agtgtgcata
     1921 tgcagccgca aaagagggaa taagaggtct tacaagagtt gccgccaacg agtggggcaa
     1981 ggacggaata aatgtcaaca ttgtctgtcc gcttgcatgg actgctcagc ttgaaaaatt
     2041 tgagcaggct tatcccgacg cattcaaggc gaatgtaaaa gtgccgccaa tgggacattt
     2101 tggtgatgtc gaaaaagaaa tcggccgtgc ctgtgttgca cttgcatcac ctgatttcaa
     2161 gtatatgagc ggcgaaacca tcacccttga gggcggaatg ggactcagac cgtaacttta
     2221 ttttaaaaat tcttttgttc tgttatttgg taaatcatta aataaaatat aaaacatact
     2281 aatatgagta ttgaattttt tgttcatatg atgtaatatc aagttacgga gtacatacaa
     2341 aaattacttt gaggtgattg tatgggaatt tttgatataa agaacacaga tattttcagc
     2401 aaggactatg actctgcaaa cgcatggctt gagtataaca tttttaatga atgtacaaag
     2461 gacgaaaacg gagagtcatt tgatgagtct gatgaaaata atttttggga ttatttaaag
     2521 aaaaataaat aaataataag ttacaaacag cgaccgcaga gtgatatgct ttgcggtcgc
     2581 ttttatgtgt tgttcacaca caaggacact cggcataata tgagggtgta gagaataact
     2641 tgatttacgg gtgttgtgta tgaaaaagag cgacataaga acatttggca taatcggcgg
     2701 tgacaagcga cagctttttc ttgcaaaatc aatttctgac agttgttacg atgttttgct
     2761 cggcggattt gacagtcttg aaagctacgg ttcattgagc ctttgcaatg ttaaaaaggc
     2821 aatttcggaa agtgatgcat tgatttttcc gttgccgtca ataaggacag acggaagtct
     2881 taacactccg ttttcaaacg agaacatttt gcttgacggt gacgatattg aaattatcct
     2941 taaaaagcct gtttttacta cgatgaaaag cagattttta aaggcatatc caaggctttc
     3001 cgacggtgaa atttttgatt acggagcaag agatgatttt gcaattctca acgcattgcc
     3061 tacggcagag ggtgcaattg aatgtgcaat gagagaatat gagggtacga tttcgggcag
     3121 taagtgtctt gtgacgggat tcggcagaat cggaaaaatt cttgctcaca agcttgtttt
     3181 actcggagca aatgttaccg tgagtgcaag aaagccgtca gaccttgcat atgtaaaagc
     3241 actcggctac aatgcactta atacggaaaa tctcagaacg gtaaagcggt ttgacattgt
     3301 tttcaacaca attccgaggt tgatttttga cagggaactt cttatgaata ccgacacaaa
     3361 cacccttatt atagaccttg cgtccctgcc gggaggggtt gattttgata ccgccgaaaa
     3421 actcggaatt tatgccgtta gggcattgtc gctacccgga aaatgtgctc cgaaaacggc
     3481 aggagaaatt ataaaaacta ctgtatttga catcatcaag gaggtttaca ggtgacaaaa
     3541 gcaaccatag gatttgcaat gtgcggttcc ttttgcacct tttcaaaggc ttttacgcaa
     3601 atgcagaggc ttgttgactc aggctatgat gttattccga taatgtcgca aaatgccttt
     3661 tcaacatcaa ctcgtttcgg aaaagcggag gaaacggtaa agagggcaga agaaataacg
     3721 ggcaaagctg ttctgcactc ggttgtgcag acagaaccga taggacctaa aaaaatgtgc
     3781 gacttgcttg tgattgcacc gtgtacgggc aacacgcttg caaagctgtc gctcggaatt
     3841 accgacacgg cggtaacaat ggcggcaaag tcgcatctga gaattttaag acctgtgctg
     3901 ttgtgcgttg caacaaatga cgctctcggt gcgtcggcac agaacatcgg cagactcctc
     3961 aacacgaaaa acatttactt cgttccttta aaacaggacg attgcatcaa gaaaccaaat
     4021 tcgcttgtgg ctgattttga taagcttgaa gattgcgtaa cccttgcatt gcagggaaaa
     4081 caacatcagc cgattttttt ataacagaac aacagccgtc ttgaaatgca aattttcaaa
     4141 acggctgtta tttgagttga caattaaaaa atagtgtgat aacttaaagt atagtatttc
     4201 tgctgaacag gagtgggtat ttaatgagaa aaagagtaaa caaaattatt tctgttgcac
     4261 tatcggcaac tcttgcgttc ggtgtgttta cagcattgcc gatgtcggca aatgcggttg
     4321 tatcaacggc aagcgaggta agtgcggagg caattccaaa acatgagctt tataaaagct
     4381 atacatatgg cggatacaaa tatcgcattg tgggtcagaa atcaaacgga agatttaacg
     4441 cgtggattga atcttattcc ggtaagtctg caagcgtcaa tgttcctgcg tcagtcggtg
     4501 attgtgatat ggtcggcatt gacaacaatt gtttttcgtt taacaaaact ttgaaaacaa
     4561 ttattgttcc caagggcatt gctgaaattg gttcaagtgc atttctcggc tgtaccgcac
     4621 ttacaagtgt aagcctgccg agtacactga caaaaataaa tttttgggca tttaaaaatt
     4681 gtacaagcct ttcgacactt tcaattccgt caagcgttgt ttcgataggt gataacgcat
     4741 ttgacaattg cctttggtac acaaatcaat ctaacggaat tgtttacgca ggaaaagttg
     4801 cgtataagta caagggtaag cttgcggata acagctctgt tacgataaaa agcggaactg
     4861 tttcaatagc cgattatgcc tttacggacc aaaagcttac atcagtcaca cttccgtcaa
     4921 gccttgtttc aatcggtgaa caggcgtttt catacactaa tctcaaaact gtgacaattc
     4981 cgaaaagtgt aagctcaatg ggttataatc cctttgcgta ctgttcacag ctttcaagca
     5041 ttacggttca aagcggaaac acaaacttct atgttcaaaa cgatttactt atcgccaaga
     5101 atcatatgta ttcagtaaca aaagatatta ctgatcctga tgcaccttct acggaatcaa
     5161 gaagctacac atcttatgct ccgtacggct cggttgtgat atcgttcccg tcagcatcaa
     5221 cgctgaaaac tgttacaatt cccgaaacgg ttaaggcaat aggcaattat gcttttgcag
     5281 gaagtaaaat cgaaaagctt actttgaaca gcggacttga atctattctc acatctgctt
     5341 tttcaggttg cacaaacctt tcatcggtga gcttttccga cagtattatc agcatttgcg
     5401 acagttcgtt tgaggaatgt acttcactta aaaatctgaa attcggcaaa aatcttgaat
     5461 ttatttcata ctatgccttt tataactgcc aaaatcttca atcggtgaca atcggggaaa
     5521 atgtaaaggc aatttgctgt gattcattcg gcaattgcaa tgctcttgta attaacggta
     5581 agattggctc aacagcagaa acctttgcca aaaagtacgg ctacaagttt aattcatcag
     5641 aaaccacaag acttaaaggc gatgttgaca ataacggtat tattaatgta gttgacgcta
     5701 ccgatattca gaaatatgtt gttaatctta cagatgaaaa tggaaataag tttattgatg
     5761 tcaacaatgc ggaagatgtg tatgtcgcag atgtaaacgg tgacggcatt atcaatgttg
     5821 tagatgcaac gcttattcaa aagtatatcg taagacttgt ggagtcactt tgataattaa
     5881 acaatttaat aaataaaaac aacagccgtc ttgaaatgca aattttcaaa acggctgtta
     5941 ttttatgttt ggattgtctg tgcgacaggt cagataatca aggcttgtgc cgtagcagtc
     6001 ggcaagttta atgagcaggg agaggggaat ttctctttcg ccccgttcat attttgagta
     6061 cagcgactgg tcgcacataa gaacctttgc aatttctttt tgcgtaaggt cattgtcctc
     6121 acgcaaatct tttattctca gtttcatttc atcaccgaat aaattataaa atagtacata
     6181 gtgacctatt gacttttagg acaaattgtc ctaaaatata attgcctgaa aaatgagaaa
     6241 acaaacggca ttatctttgc ggtactcata taatcgtatt tatcataaat tgtgtattat
     6301 tatttgcaca agtgtccaat gagccgcaaa tacgagttga tgattacttt gaaaaattta
     6361 attcacctta aataagagcg ttgcttaaat aaaaataata ttttatctgt atatatattg
     6421 acaaataccg caaaatgcag taaactaatg tcataaaaat cccgaagaac atcttcggga
     6481 cgaatagctt gtcgatttaa ttgtgcgtat aattcggtaa gtctttgttg tcagtcagcc
     6541 ccaaaatata gtcggtgctg gtgtgataat gttttgcgag gcaaatcaaa acaacggtgg
     6601 ggatatcacg ctgaccaagt tcataattgc tgtaaacctg ttgtgaacaa tgcagaattt
     6661 tgctaacttc tttttgcgtg aggtcggcat cttccctcag ttccctgatt cttttataca
     6721 cagaattcac ctcgcataca gtatagcata ccgcagattg ttgtattgca atataccgca
     6781 aaatgcagta aaacagcaat ggtattttgg gttaattttt aaataattat tcagagggga
     6841 atggtgttta aaacaaaaat attaatcttg catatcagat aaaacaataa aatgatggag
     6901 gaaattatga gaaaacattc aaaatttatt acgattttgc tgtctgcttt gttggcggta
     6961 tgtcttatgc agacagtatt tgttcaggca tttgcaaccg aaatgggcga tgatgacttt
     7021 ggttggggca atgcatcaac aagtgatgaa gcagagacgg tatatcctgt ttcaatggaa
     7081 gttgttaaag cacccgaaag cgttactgtt aataatggtc gaattaccga cagcactttt
     7141 tgg
//
LOCUS       DAOMVE010000098         7141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2784_length_7141_cov_3.436212, whole genome shotgun sequence.
ACCESSION   DAOMVE010000098 DAOMVE010000000
VERSION     DAOMVE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2784_length_7141_cov_3.436212"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLS64_06450"
     CDS             complement(<1..501)
                     /locus_tag="PLS64_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43496.1"
                     /translation="MDFKEKLKARNPESTLADMDDIAFLRDLALVKSDSGNIKLTVAG
                     LLFVGKEQAINRLLPQAEVIYLHYSESNLEEYDARLDMKAPIISVIDRLSEKIQDSNR
                     IVNVQVGLFRLEIVDFPEKVFQEALLNALSHRDYQSQGAVYVKHYPDKIVIENPGAFL
                     DGITENN"
     assembly_gap    526..539
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<540..1013)
                     /locus_tag="PLS64_06455"
     CDS             complement(<540..1013)
                     /locus_tag="PLS64_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL43497.1"
                     /translation="MNIDDFAEILKIGESINTEFKSWNKVSDMKKRINLAVDELIAFA
                     NNKGGTLYFGVEDNGEVTGCDGNYDLQNIIESIYEKTRPSIFVDPEEIEYNGKKVIAL
                     TVASDGITHATTDGRCLKRLGKNSKPFYPDEMSNRYSEIQSSDFSGRILSDSTEDD"
     gene            complement(1673..3496)
                     /gene="asnB"
                     /locus_tag="PLS64_06460"
                     /pseudo
     CDS             complement(1673..3496)
                     /gene="asnB"
                     /locus_tag="PLS64_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010168225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
     gene            complement(3631..4086)
                     /locus_tag="PLS64_06465"
     CDS             complement(3631..4086)
                     /locus_tag="PLS64_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRL43498.1"
                     /translation="MTKGEIAKNNFMQGYNCAQAVLLAFCDDLGFDEQTALMLASPFG
                     GGIGRMREVCGTVTGMYMALGLARGYSDSKDNADKKRVYTEVQQLAERFKEDNGSIIC
                     RDLLCMRIKAKDNPTPSERTEKYYAARPCPELCRYAADLLDEYLKNKKG"
     gene            4617..6428
                     /gene="lepA"
                     /locus_tag="PLS64_06470"
     CDS             4617..6428
                     /gene="lepA"
                     /locus_tag="PLS64_06470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRL43499.1"
                     /translation="MSFPQDKIRNFSIIAHIDHGKSTLADRILEQTNSVSERDMEAQL
                     LDDMDLERERGITIKARAVSVIYKADDGEEYLFNLIDTPGHVDFNYEVSRSLAACEGA
                     ILVVDASQGIEAQTLANTYLAVDSGLEIVPVVNKIDLPSADPDRVIQEIEDVIGIPAE
                     DAPKISAKAGINIHAVLEKVVHDIPAPTGDINAPLRALIFDSYYDSYKGVIIYVRLKE
                     GQIHPGDEFKLMATGSVFNVVECGLMHATAPEKTDGLYAGEVGYITGSIKTLADAKVG
                     DTVTLTSNPAEEPLPGYREAQPMVFCGIYPVDGARYGDLKDALEKLQLNDAALSFDPE
                     TSVALGFGFRCGFLGLLHMEIIQERLEREYQLDLITTAPSVIYRITRTDGTVEMIDNP
                     TNYPDPSLIQMAEEPVTDAHIYAPKEYVGNIMELCQDRRGTFVDMQYIDADRVDLHYV
                     LPLAEIIYDFFDTLKSRTRGYASFDYELKEYVQSNLVKLDIMLNGEVVDALSFIIHAD
                     KAYSRARKMAEKLKEKIPRQLFEIPIQACIGGKIIARETVKAMRKDVLAKCYGGDISR
                     KKKLLEKQKEGKKRMRQLGSVEVPQEAFMAVLKLDTD"
     gene            6415..6828
                     /locus_tag="PLS64_06475"
     CDS             6415..6828
                     /locus_tag="PLS64_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HRL43500.1"
                     /translation="MIRINGEDMKELAMFYNFSDERFRKAKFALMPLKIQVKKVEKED
                     FNQPIGFLAGIKGVEPVAEKFDGDGFDEEMIVMHNFTNKTIDSLIRALNKCGVGRVPL
                     KAVITPTSKDWDSLTLIKALKADHEEMLKKLKTKS"
     gene            complement(6927..>7141)
                     /locus_tag="PLS64_06480"
     CDS             complement(6927..>7141)
                     /locus_tag="PLS64_06480"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRL43501.1"
                     /translation="LLRLCDFLNIGMENVCACGNADNDADMIAESGLGAAVENASQKC
                     LDRADIVIPSNNNDGVAQLIEKILKN"
BASE COUNT         2000 a         1600 c         1433 g         2094 t
ORIGIN      
        1 attattttct gtgataccgt ccaaaaatgc accggggttt tctattacaa ttttatcagg
       61 ataatgcttt acataaaccg caccttgact ttgataatct ctgtgtgaca aagcattgag
      121 taatgcctct tgaaaaactt tttcagggaa atcgacaatc tccaatctga aaagtccaac
      181 ctgaacgttg acaatacgat ttgagtcttg tattttctct gaaagtctgt caattaccga
      241 tataatagga gccttcatat caagtcttgc atcatattct tctaaattcg actcggaata
      301 gtgcaagtaa attacctctg cctgtggaag taatctattg attgcctgct ctttgcctac
      361 aaataataat ccagccacag tcaatttaat attaccgctg tcagacttaa ccaaagcaag
      421 gtcacgcaaa aatgcgatat catccatatc agctaatgtc gattcgggat ttctcgcttt
      481 taatttttct ttaaaatcca tataatctcc ttacttccta tgtctnnnnn nnnnnnnnna
      541 tcatcttctg tgctgtctga caaaatcctt cccgaaaaat cggagctttg aatttcggaa
      601 tagcggttgc tcatttcatc gggataaaac ggtttagaat tttttccaag tctttttaag
      661 catctgccgt ctgtagttgc atgtgtaatt ccgtctgatg caacggtaag tgcaatcacc
      721 tttttaccgt tgtattctat ttcttcggga tcaacaaaaa tagacggtct tgttttttcg
      781 taaattgatt caataatgtt ctgcaaatcg tagttaccgt cacaacccgt aacttcaccg
      841 ttatcttcaa cgccaaagta aagagtgcct cctttgttat ttgcaaaagc tataagttca
      901 tcaaccgcca aattaattcg tttcttcata tcagacactt tattccacga tttgaattct
      961 gtattaatcg actcaccgat tttcagaatt tctgcaaaat catcaatatt caaaaaagca
     1021 ccccttttct ttgcagagca gtcattatag tattatttga ttattcatct tatttataaa
     1081 atttaagggc aacctcggtt gcccttgttt attttgttat ttttaataac tttgattaat
     1141 ttttatcgtc ctcatcatca aagaaagaga ttgagtttac gatattgtca acagcctcgt
     1201 ctgaaattac attcggctcg tcaagatatt ttgatctttt ttcaatgctg tcaatagcca
     1261 tctgcatctg ctcctgggga gttgacggtt ttttcggagc gtcgtaaatc tcgttatcct
     1321 ccggatttga gatgttcaca tactcctctt ttttaggctc ttctttaacc tcgggttctt
     1381 cctcaggctc aacgattgca ggctcattgc cgtcatcggg aacttcaact tcctctgtca
     1441 catcttccgc agattcctct tttacgggtt cttccgcttt ttcggcagat tcatcatttt
     1501 tttttgactt gtcaacgcca aagttcggag catcgtaatt ttcgtcaatc tcctctgcgg
     1561 cagccataat tacctcaccg ggagcgtcaa tctgcttttc tcttctcgcc tcggcaacag
     1621 ccttgagttc atcaaggtga tttacaggct tttcaacctt ttcgctgtcc tcatcaaaat
     1681 agatatcata ccatacgagg aacacataaa ttgtccaaac tcgtctgctg ttatcttctt
     1741 ttccgctgaa atgactgtcg agccatgaaa caaggacatc tgtattgaag aatttctcgg
     1801 cagttttgcc cttgaacttt gtctttacaa cattataata cttctcgtca cgaagccata
     1861 ctcttgtcgg tacagggaaa ccgagtttct ctttttcggc agtagcctca ggcatatggc
     1921 gaacggcagc cttacgcatt gcatactttg tgttgtgctt gttgcagcgc agttttgtcg
     1981 ggagcgatga cgcaaccttg aaaacctcct tatcgaggaa aggaacacga agttcaagcg
     2041 agttagccat actcattcgg tcggctttaa gaaggatgtc gccgaccatc cacatattga
     2101 tgtcgagata ctgcattttt gtaacatcgt catacttacg gcaacgataa taatattttc
     2161 ttgtaagatc ctgcgggcgt gttgcaattg acggatcttt caaaagctcc tgcttctgct
     2221 tgtagtcata cataaatgcg ttaccgatat aacgctcctc aagcttgtca caggcacgga
     2281 taagataatc tctgcccttg atgtcgggga gctttctgca aattgctctt attgcctttc
     2341 tgatgcaatg cggaacaatt gtctgataaa ttttgaacac tctcggatcg ttgtagcaag
     2401 tgtaaccgcc gaacaattcg tctgcaccct cacccgaaag gacaaccttt acataatggc
     2461 ttgcaagacg ggatacaaag taaagtgcga tacatgacgg atccgcaagc ggctgatcca
     2521 tatggtactg aacagtcgga acagcgtccc agaattcttc acttgaaata aggtgagtgt
     2581 ggtgttcaac accgattttt tcgctgagat tttttgccca gctgatttcg ttatattttt
     2641 cgccaaagtc aaagccgact gtaaatgttt tctggtcggc aaaatatgtt gaaacatagc
     2701 ttgagtcaac accgcttgaa aggaaacaac caacctcaac atcggcaatt ttgtgagcat
     2761 ttacggagtt ttcaaaaacc ttttcaattc tgtcaacagc ctcttcctcc gtcatttctt
     2821 cgtcgggatt gaaacgagcc tcaaagtaac gggtagtttc aacctcgccg tccttgtacc
     2881 agagatagtg accgggcata aggcagtaaa caccctcaaa gaaagtttcg ggcggaactg
     2941 catactggaa tgaaaggtac gagtcaagtg cctttctgtt gaatttcttt tcaaacttag
     3001 gatattcaag aatactcttg atttcacttg catagacaaa gtcattgcct atctgagtgt
     3061 agtgcatcgg cttaatgccg aaaaagtcac gggcaataaa aagtgaatta tcctttgtgt
     3121 tgtaaatagc aaagccgaac ataccacgga gttttgggag catatcttct ttccactctt
     3181 caaagccgtg aacaagcact tcactgtctg tatctgtata gaacttatga ccttttgcga
     3241 taagttcttt tctcaaatcc ttgtagttat aaatttcacc gttgaatgtg agaacaaggc
     3301 ttttatcctc attgtatata ggctgatgac ctgcaccgag gtcaataatg gaaagtctgc
     3361 gaaatcccat ggaaatattg tcgtccgtaa aaaatccgtc cgagtcggga ccacggtgag
     3421 tgataacctc tgtcatattt ctgatgacat cggctctgtt ttcaacctgt cccataaagc
     3481 cggctatgcc gcacatatca caattcccct ttcaaggtaa ttttcaaaat taaccgcatt
     3541 agactaatgc cctattttaa acattttatc acaatttact cccaaattca actgtttgca
     3601 ggcataagtt tcaaatcaaa gcaacgcccc tcagcctttt ttattcttta agtattcatc
     3661 aagcaaatcc gctgcatatc tgcacaattc aggacacgga cgggcggcat aatatttctc
     3721 tgttcgctca gacggagtcg ggttatcctt tgcctttatt ctcatacaga gaaggtcacg
     3781 gcagatgatt gaaccgttgt cctctttgaa cctttcggca agctgctgaa cctcggtgta
     3841 gacacgcttt ttgtcggcat tatccttgct gtcactgtaa ccccttgcaa gacctaaagc
     3901 catatacatt cccgtaacag taccgcagac ctcacgcatt ctgcctattc cgccgccaaa
     3961 cggcgatgca agcataagag cagtctgttc atcaaatccc aaatcgtcac aaaatgcaag
     4021 caatacagcc tgagcgcagt tgtagccctg cataaaatta ttcttagcaa tttcaccttt
     4081 tgtcatacca atttctcctt ttatagggtt atttcatata tgcccagtat agcaccgaaa
     4141 accgcatact gcaacgattt tttgcttgtg aaagagattg ccatatgtta taatttaggg
     4201 agttatgatg ataaataaat caatattgct taaccgtagc ggcggtcagt gaccgccatt
     4261 cgctttggtt gcaaaaccaa aacgaacaaa tatacatctg caataagcgt tttaatcaat
     4321 aacatacgat aaacaaatgg tttgtattta atcgaaccga ttttacaatc gtttcgattg
     4381 gcgttcactg aacgccgcta ctgtttgtac aaacggtaac aatgctacgc tcgggcaaaa
     4441 tatgttgcgg atttttcaaa ctgtctgcac gaatagggaa cggcgctact gatttgagtc
     4501 acaatttctc ccacagattt aatcaaatac ggttgcttta ttcggcagaa ttggtataat
     4561 aatttacaga acataactca ttaaaattca gacattaaag aaaaaggtga ttagcattgt
     4621 cttttccaca ggataaaata agaaatttca gcattattgc tcacattgac cacggcaaga
     4681 gtacgcttgc ggacagaatt cttgagcaga ccaactccgt ttccgaaagg gatatggagg
     4741 cgcagctgct tgatgacatg gaccttgagc gtgagcgtgg cattacaatc aaggcgagag
     4801 cggtaagcgt tatctacaaa gccgatgacg gcgaagaata tctttttaac ctcatcgaca
     4861 cccccggtca tgttgacttc aattacgagg tttcccgttc actggcagct tgtgagggcg
     4921 ctattcttgt tgttgacgca tcacagggca ttgaggcgca gactcttgca aacacatatc
     4981 ttgccgttga cagcggactt gaaattgttc ccgttgtcaa caaaattgat ttgccgagtg
     5041 ccgaccccga cagggttatt caggaaatcg aagatgtaat cggtattcct gccgaggatg
     5101 ctccgaaaat ttccgcaaag gcaggaatca acattcacgc agttcttgaa aaggttgttc
     5161 acgatattcc tgctccgaca ggcgacatca acgcacctct gcgtgcattg atttttgaca
     5221 gctactacga cagctacaag ggcgtaatta tatatgtaag gctcaaagag ggacagattc
     5281 acccgggcga tgaattcaag cttatggcaa cgggcagtgt gttcaatgtt gttgagtgcg
     5341 gacttatgca cgcaaccgcc cccgaaaaga ccgacggtct ttatgcaggc gaggttggct
     5401 acatcacggg ttcaatcaag actcttgcag acgcaaaggt tggcgacacg gttacactca
     5461 catcaaatcc cgcagaagaa ccgctccccg gctaccgtga ggcacagcct atggttttct
     5521 gcggtatcta ccctgttgac ggcgcaagat acggcgacct taaagatgca cttgaaaagc
     5581 ttcagcttaa cgatgcggct ctttcgtttg atcccgaaac atcggttgcc ctcggattcg
     5641 gtttccgttg cggatttttg ggactgctcc acatggaaat cattcaggag cgacttgaaa
     5701 gagaatatca gcttgacctc atcacaaccg caccgagtgt aatttacaga atcacacgca
     5761 cagacggcac agttgagatg attgacaacc cgacaaacta ccccgatccg tcacttattc
     5821 aaatggcgga agagcctgta acagacgctc acatctatgc acctaaggaa tatgtcggca
     5881 atattatgga gctttgtcag gacagacgag gaacttttgt cgatatgcag tatattgatg
     5941 ccgaccgtgt tgatttacac tatgtacttc cgcttgccga gataatctac gactttttcg
     6001 acacgctcaa atcacgcaca agaggttatg catcattcga ctatgagctt aaagaatatg
     6061 ttcagagcaa ccttgttaag ctcgatatta tgctcaacgg cgaggttgtc gatgcacttt
     6121 cgttcatcat tcacgccgac aaggcatatt caagggcaag aaagatggct gagaagttaa
     6181 aggaaaagat tccgagacag ctctttgaaa ttccgattca ggcttgcatc ggcggtaaaa
     6241 ttattgccag agaaaccgta aaggctatgc gtaaggatgt acttgccaag tgctacggcg
     6301 gtgatatttc ccgtaagaaa aaactccttg aaaagcaaaa agaaggtaaa aagcgtatgc
     6361 gccagctcgg ttctgttgaa gttccgcagg aggcatttat ggcggttctc aagcttgata
     6421 cggattaacg gtgaagatat gaaagaactt gctatgtttt acaacttttc ggacgaaaga
     6481 tttcgcaagg caaagtttgc acttatgccg ttgaaaattc aggttaaaaa ggttgaaaaa
     6541 gaagatttca atcagcctat cggtttcctt gcaggcatca agggcgttga gcctgtggca
     6601 gaaaagtttg acggtgacgg ctttgacgaa gaaatgattg tcatgcacaa tttcacgaat
     6661 aaaacgattg actcgctaat cagagcgctg aacaagtgcg gtgtcggacg agttccgctc
     6721 aaggcggtta tcactcccac aagcaaagat tgggacagtc tgacgcttat caaagcactt
     6781 aaagccgacc atgaggaaat gcttaaaaaa ttgaaaacga aaagttgaaa atggaaaatg
     6841 aatgtcggga agacagtttg caaaatgcaa aacaatgtct tcccgactgt ttttatttat
     6901 atgaaattac cgccgcatta ttacaatcaa tttttaagaa ttttctcaat caattgtgca
     6961 acaccatcgt tattatttga cgggattacg atatcggcac ggtcaaggca tttttgactt
     7021 gcgttttcaa cagccgcacc aagtcccgac tcggcaatca tgtcggcatc attatcggca
     7081 tttccgcagg cgcagacatt ttccatgccg atattcaaaa agtcacacaa acgcaggagt
     7141 g
//
LOCUS       DAOMVE010000099         7136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2788_length_7136_cov_3.652874, whole genome shotgun sequence.
ACCESSION   DAOMVE010000099 DAOMVE010000000
VERSION     DAOMVE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7136
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_7136_cov_3.652874"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLS64_06485"
     CDS             complement(<1..417)
                     /locus_tag="PLS64_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRL43502.1"
                     /translation="MNKYKYYLFDLDGTISESALGIRESLENAIKSMGKPLPNLDDYT
                     LYIGPPLLDTFRNICHFNEEESARGVEIYREYYNTKGKLVNKLYDGIDRVLAELKNSG
                     AKLAVCSSKYEKFAEEITDLLGVHDMFDAICGSTLDG"
     gene            complement(410..799)
                     /locus_tag="PLS64_06490"
     CDS             complement(410..799)
                     /locus_tag="PLS64_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HRL43503.1"
                     /translation="MSEINTYSVTIKVEEENLAVSMGSGSLCVLATPCVVALMENAAA
                     ALADKVLDNDELTTVGTMISIEHTSPTPLGAEVTATAVLKSVDGRMFNFEVFAEDKKG
                     EIARGTHTRVSVKSEKFQMKADSKFDE"
     gene            965..2059
                     /gene="ychF"
                     /locus_tag="PLS64_06495"
     CDS             965..2059
                     /gene="ychF"
                     /locus_tag="PLS64_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRL43504.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITDAGAQSANYPFCTIEPNIGVVAV
                     PDKRLDKLAEMYDPDKYTPASIEFVDIAGLVKGASKGEGLGNKFLSNIRECDAIVHVV
                     RCFENDDIIHVEGNINPARDIETINLELILSDVEMLERRIDKTKKMLKGDKKYQKDID
                     LFERVLAALNEGKPARSVECDDDEKAILSEVALLTNKPVIYAANMSDEDFSNGIDSNE
                     GYKAVQKIAAEEHAGVLPICAQIEEEIVEMDKEEKEMFLADMGLEESGLDRLIKECYA
                     LLGLISYLTAGKQEVRAWTIKKGTKAPQAAGKIHSDFERGFIRAEVIAYDDLIACGSM
                     TAAKEKGLVRSEGKDYVMKDGDVVLFRFNV"
     gene            2173..2301
                     /locus_tag="PLS64_06500"
     CDS             2173..2301
                     /locus_tag="PLS64_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43505.1"
                     /translation="MCNFGNGNGCCSWIILLIIVLCCCGGNNGGCGCDNNSCGCGC"
     gene            complement(2485..3435)
                     /locus_tag="PLS64_06505"
     CDS             complement(2485..3435)
                     /locus_tag="PLS64_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HRL43506.1"
                     /translation="MAQVIKKATRESFGMALCELAKTNKDIIVFDADLAAATKTGIFK
                     KEFPERFFDCGIAEGNMVGVAAGMAAAGKIPVAASFAMFATGRAFEQIRNSVAYPHLN
                     VKIAGSHAGISTGEDGATHQCLEDIGIMRTIPGMVVLNPADHYEMMAATKAAIEYNGP
                     VYIRLGRLAVDSFNDPDTYTFELGKGITLHEGNDVAVIATGLVVNEALKAVKELEAEG
                     INARLINIHTIKPIDRDIIIKAAKETGRIITVEEHNVIGGLGDAVCDVVSAECPVKVT
                     KIGVNDRFGYSGPALELLDKFGLTQPHIAKVVRDVVKEDK"
     gene            complement(3435..4283)
                     /locus_tag="PLS64_06510"
     CDS             complement(3435..4283)
                     /locus_tag="PLS64_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRL43507.1"
                     /translation="MDSSEIKQLRILAARSRMGAILGTYHAKSGHPGGSLSAADIFTY
                     LYFKEMNVDPKNPDMQDRDRFVLSKGHCCPALYATLALKGFFEWDELTKLRHVGAMLQ
                     GHPDMKGTPGIDMSTGSLGQGISAACGMALAAKLDNKSYRTYTVLGDGEVEEGQVWEA
                     AMFAAHNKLDNLVVIVDQNGLQIDGTVEEVAGIEPLDKKFESFGFEVIKIDGHDFNQI
                     ESALEKAKTVKGKPTAILAKTIKGKGVSFMENQVGWHGTAPNKEQYEQATAELQAEID
                     RLEGNC"
     gene            4526..5293
                     /locus_tag="PLS64_06515"
     CDS             4526..5293
                     /locus_tag="PLS64_06515"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRL43508.1"
                     /translation="MLLAADVGNTNVKLGIFDGDNLKFKLRFSTDENKTSDELAVELY
                     TFLQIYNIDAKSIDGAIISSVVPKFTYNLRRAIMTVTDRKSLLIGPGLKTGLDIKIEH
                     PETLGADIVAGCVGACEKYGGPLIMIFMGTATAIVYVDASNAYHGGAIAPGMGISLDA
                     LTNHGALLSSVDMKAPKKVISSNTSDCIRSGIMFGTACMLDGMVDMFTEECKTECKVI
                     ATGGLAPLAIKNCRHDIIFDENIILDGLKIIYERNKK"
     gene            5418..6035
                     /locus_tag="PLS64_06520"
     CDS             5418..6035
                     /locus_tag="PLS64_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRL43509.1"
                     /translation="MNKTGNFSKVQRMVILAVLIALTLVMALTPLGYLKIGALSISFL
                     CIPIAIGATVLGPTAGLILGTLFGITSFVQCFGMDAFGTAMLSINWFYTFIICVVARA
                     LMGWFTGLIAKGLGKVFEGKTVGKFNLNDLIATILCPVMNTAFFLGFMAILFGSIEVM
                     GLNVITAVVIPALSINCILEIIACFVVGSAVSIAVKKIVAANKSK"
     gene            6112..7080
                     /locus_tag="PLS64_06525"
     CDS             6112..7080
                     /locus_tag="PLS64_06525"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRL43510.1"
                     /translation="MQKKIIELLTKTDGYVSGSEISEQLGVSRQAVWKAINNLKEQGY
                     KIDSVTNRGYRLVAYPPHLNEPAIEKYLKTSVIGKNLIVLDEVGSTNDYLKKLGADGC
                     ENGTVAVAREQTQGKGRLGRVWKAKKDDSITFSFLLRPKIAPSEVSSITPLAGLAVCK
                     AIREFTGLDCRIKWPNDIIIGKKKLVGILTEMSAEFDAVEYVITGIGINADTQDFPDD
                     IKEKATSIYLATGGHFDKNKFFACVLNHIEEEFIKNDLKLSPVALKEYTSLCATVGRD
                     ITFTKDGKSVTAKAVGVNPDGELIAELPDKTTKLINSGEVTVQGIY"
BASE COUNT         2060 a         1581 c         1504 g         1991 t
ORIGIN      
        1 gccgtcaagt gttgaaccgc agattgcgtc aaacatatca tgcacaccga gaaggtctgt
       61 aatttcttcg gcaaactttt catactttga cgaacacacg gcaagctttg caccgctgtt
      121 tttaagctct gcaagaaccc tgtcaatgcc gtcataaagc ttgttcacaa gcttgccctt
      181 ggtgttgtag tattcacgat aaatctcaac gcctcttgcc gattcttcct cattgaaatg
      241 gcagatattg cggaatgtat cgagcaacgg cggtccgatg tacaatgtgt aatcgtcaag
      301 attcggcaac ggcttgccca tacttttaat tgcgttttca agtgactctc ttatgcccaa
      361 tgcgctctcg ctgattgtgc cgtcaaggtc aaacagataa tatttgtact tattcatcaa
      421 acttgctgtc cgccttcatc tgaaattttt ctgattttac gcttactctt gtgtgagtgc
      481 ctcttgcaat ttcacccttt ttatcttcgg caaaaacttc aaaattaaac attctgccgt
      541 caacgctttt gagaacggct gttgccgtaa cctcagcacc gagaggagta gggcttgtgt
      601 gctcaattga aatcattgta cctactgttg taagctcatc gttgtcaaga accttgtctg
      661 caagagctgc ggcggcattt tccataagag ccacaacgca cggagttgca agcacacaaa
      721 gactgccgct gcccattgaa acggcaagat tttcttcttc aaccttaatt gttacagaat
      781 atgtatttat ttcgctcata aaaatttcct ttcttattgt ttaaagcaat tttatgtgat
      841 atacttaaca aagtgtgcat tactcgtctg tgaattgaca gagtaagtat ccatacgata
      901 taataataca atattcagtg aaaataggca agcattgccc gaatattatt tgaggtgaaa
      961 ataaatgaaa ctcggaatcg tcggacttcc aaatgtcggc aaaagtacac tttttaacgc
     1021 aattacagat gcaggtgctc agagtgcaaa ctatcctttc tgcacaattg aaccgaatat
     1081 cggtgttgtt gcagttccgg acaaaagact tgataaactt gcggaaatgt atgatcctga
     1141 caaatatact cctgcatcaa ttgaatttgt agatattgca ggtcttgtaa agggtgcgtc
     1201 aaagggcgag ggactcggca acaagttcct ttcaaacatc cgtgaatgtg acgctatcgt
     1261 tcatgttgtc cgttgctttg aaaacgatga cattatccat gttgaaggca acatcaatcc
     1321 tgcccgtgac attgaaacaa ttaatcttga gcttattctc tccgatgttg aaatgctcga
     1381 acgcagaatt gacaagacaa agaaaatgct caagggcgat aaaaagtatc agaaagacat
     1441 cgaccttttt gaaagagttc ttgccgcact caacgagggc aaacccgcaa gaagtgttga
     1501 atgtgacgat gacgaaaaag caattctttc agaggttgcg cttctcacaa acaagcctgt
     1561 tatctatgcc gcaaatatga gtgacgagga tttctcaaac ggcatcgact caaacgaggg
     1621 ctacaaggct gttcagaaga ttgccgccga ggaacacgcc ggcgttcttc caatctgtgc
     1681 tcagattgag gaagaaatcg ttgaaatgga caaggaagaa aaggaaatgt tccttgccga
     1741 tatgggactt gaggaaagcg gtcttgacag acttatcaag gaatgctatg cacttctcgg
     1801 acttatctcc taccttacag ccggcaaaca ggaggttcgt gcatggacaa tcaaaaaagg
     1861 aacaaaagca cctcaggcag caggcaaaat tcactcggat tttgagcgtg gatttatcag
     1921 agccgaggtt atcgcatatg acgaccttat cgcctgcgga agtatgacag ccgcaaaaga
     1981 aaaaggtctt gtccgcagtg aaggcaagga ctatgttatg aaagacggcg atgtcgttct
     2041 tttcagattc aatgtttaat taaatcatta caattgatga cggttacgaa tattttcgca
     2101 gccgtcattt tttgatgtca cacatttctc cttttgcaat aatatgtagt gtaaacgaaa
     2161 ggaggtaagc gtatgtgtaa ctttggtaac ggcaacggtt gctgctcatg gattattctt
     2221 cttatcatcg ttctctgctg ttgcggcgga aacaacggcg gctgtggttg tgacaacaac
     2281 agctgtggct gcggctgctg ataaagcacg ctgactaaag ccccattcgg cttattgctg
     2341 aggatgtttt aatataaaaa gggcggctgc cgttttttgc gatatccgcc ctttgctttt
     2401 tattcttgaa cggtacatac aaaaaacggt cggcttgaac aaaccgaccg ttaatatttt
     2461 gcaattatta aattaattca gaaattattt gtcctcttta acaacatctc tgacaacctt
     2521 tgcaatgtga ggctgtgtaa gaccgaactt gtcaagaagc tcaagagccg gaccgctgta
     2581 accgaaacga tcgttcacac cgatctttgt aaccttaaca ggacactctg cacttacaac
     2641 atcacaaaca gcgtcaccga gaccgccgat aacattgtgt tcctcaacgg tgatgattct
     2701 gcctgtttcc tttgcagcct taatgatgat atctctgtcg ataggcttta ttgtgtgaat
     2761 gttaataaga cgagcgttga tgccctcagc ctcaagttcc ttaacagcct ttaaagcctc
     2821 gtttacaaca agtcctgttg caataacagc cacatcgttg ccctcatgga gagtaatacc
     2881 cttgccgagc tcaaatgtat aggtatcggg atcattaaaa ctgtcaacag caagtctgcc
     2941 gagacggata taaacaggac cgttgtactc aattgcagcc tttgtagcgg ccatcatttc
     3001 gtagtgatcg gcagggttaa ggacaaccat acccggaatt gtacgcataa taccgatatc
     3061 ttcaagacac tgatgagtag caccgtcttc acctgttgaa atacctgcgt ggctgccggc
     3121 aattttaaca ttaagatgag ggtaagcaac cgagttgcga atctgctcaa atgctctgcc
     3181 tgttgcaaac attgcaaatg atgcggcaac cggaattttg cctgccgctg ccatacctgc
     3241 cgcaacgcct accatattgc cctcggcaat accgcagtca aaaaatcttt cgggaaactc
     3301 ttttttaaaa attcctgtct ttgtagcagc tgcgaggtct gcatcaaaaa caataatgtc
     3361 cttatttgtc tttgcaagct cacaaagagc catgccaaag ctttctcttg tagccttttt
     3421 aattacctgt gccatcagca gttgccctcc aatctgtcaa tttcagcctg aagctcggca
     3481 gtagcctgtt catactgttc cttgttggga gcagtgccgt gccagccaac ctgattttcc
     3541 ataaatgaaa cgcccttgcc cttgattgtc ttggcaagaa ttgcggtagg cttgcccttt
     3601 actgtcttag ccttttcgag agccgattca atctgattga agtcgtgacc gtcaattttg
     3661 attacttcaa agccaaagct ttcaaacttt ttatcaagag gctcaatgcc tgcaacctct
     3721 tcaacagtac cgtcaatctg aagtccgttc tggtcaacaa ttacaacgag attgtcaagc
     3781 ttgttgtgag cggcaaacat tgcagcctcc caaacctgac cttcttcaac ttcaccgtca
     3841 ccgagaactg tatatgtgcg atagctcttg ttatcaagct tagccgcaag tgccataccg
     3901 caggcagccg aaatgccctg accgagtgag cctgtactca tatcaatacc gggagtaccc
     3961 ttcatatcgg gatgtccctg aagcatagcg cctacatgac ggagctttgt aagctcgtcc
     4021 cattcaaaaa agcccttgag tgcaagtgtt gcataaagag ccggacagca gtgacctttt
     4081 gaaagaacaa aacgatccct gtcctgcata tcgggatttt taggatcaac attcatttcc
     4141 ttaaagtaga gatatgtgaa aatatctgct gccgaaagcg atccgccggg atgacctgat
     4201 ttggcgtggt aagtgccgag aattgcgccc attcttgacc ttgcggcaag aattcggagc
     4261 tgcttgattt ctgaactgtc catttaataa accattcctt tcagaatttt gcgtaataca
     4321 tgaccgtgtt atacacacct attttaatta tacacaatcc aacgcaaatt tcaacaattt
     4381 tttaggattt tccaaaaatc ggataaattc cttaaattca cccttgaaga aataattttt
     4441 tatgatataa taagcaaagt aaaatacgat taaatctaaa cttttgtaaa agcatattta
     4501 taaaaaccgt ttggagggaa cagcaatgct gcttgcggcc gatgtcggaa acaccaatgt
     4561 caagctcggc atttttgacg gagataattt aaaattcaaa ctccgttttt caaccgatga
     4621 aaacaaaaca agcgacgagc ttgccgttga actttacact tttttgcaga tttataacat
     4681 cgacgcaaaa agcattgacg gcgctattat aagctctgtt gttccgaaat ttacatataa
     4741 tctcagacgt gcgattatga ccgttacaga cagaaaaagt ctgctcatcg gtccgggact
     4801 caagacaggt cttgacatca agatagaaca tcccgaaacc ttgggtgcag atatcgttgc
     4861 cggctgtgtg ggagcgtgtg aaaaatacgg cggtcctctc attatgattt ttatgggaac
     4921 tgcaaccgca attgtctatg ttgacgcaag caacgcgtat cacggcggtg caatcgcccc
     4981 cggtatggga atttcgcttg acgcactcac aaatcacggc gcattgcttt catcggttga
     5041 tatgaaagct ccaaagaagg taatttcatc aaacacatcc gactgcattc gttcgggaat
     5101 tatgttcgga acagcctgta tgcttgacgg aatggttgat atgtttaccg aggaatgcaa
     5161 gacagaatgc aaggttattg ccacaggcgg tcttgctccg cttgcaatca aaaactgccg
     5221 tcacgatatt atctttgatg agaatattat tcttgacgga ctcaaaatta tttacgagag
     5281 aaataaaaaa taaagacaat ttaatataca acttgttttt gaattattaa tttgatgcaa
     5341 cggggttctc cccttgtaaa tattatttgc gtttcacctg cggaaaatga gcagggaaac
     5401 agcaggagga attttttatg aataagacag gaaacttttc aaaggtgcaa agaatggtta
     5461 ttcttgcagt gcttatcgca ttgacgcttg taatggcgct cacaccgctc ggatatctga
     5521 agatcggtgc gctttccatt tcattccttt gcattccgat tgcaatcggt gcaaccgttc
     5581 tcggtcctac agcaggattg attctcggca cactctttgg tatcacaagc tttgtacaat
     5641 gctttggtat ggacgcattc ggcacggcaa tgctttcaat caactggttt tacaccttta
     5701 taatttgtgt tgttgcacga gctcttatgg gttggttcac aggacttatc gcaaaaggtc
     5761 tgggcaaggt atttgaaggt aaaactgtag gcaagttcaa tcttaacgac cttatcgcta
     5821 ctatactctg ccctgttatg aacacagcct tcttccttgg ctttatggca atccttttcg
     5881 gcagtatcga agttatggga cttaatgtaa tcacagccgt ggttatccct gctctttcaa
     5941 tcaactgcat tcttgaaatc attgcttgct ttgttgtcgg ttcggcagta agcattgcag
     6001 tcaagaaaat tgttgcggca aacaaaagta aataattaac attcaaaaac gggcgatttt
     6061 gtttagaatc gtccgtaatt ttttacatta aattttaaaa tcggtgatgt tatgcaaaag
     6121 aaaattattg aacttttaac aaaaacagac ggctatgttt cgggcagtga aatttccgaa
     6181 cagcttggtg tgtcaagaca ggcagtgtgg aaagcgatta acaatctcaa ggaacagggc
     6241 tacaagattg actctgtaac aaaccgtgga taccgccttg ttgcctatcc gccacacctc
     6301 aacgagcctg caatcgaaaa atacctgaaa acctccgtaa tcggcaaaaa cctcatcgtc
     6361 cttgacgagg ttggttcaac aaacgattac ctcaaaaagc tgggtgcaga cggttgcgaa
     6421 aacggcacgg ttgccgttgc cagagaacaa actcagggca agggcagact cggcagagtt
     6481 tggaaagcaa aaaaggatga cagcatcacc ttttcgtttt tgttaagacc aaagattgcc
     6541 ccgtcagagg tatcgtcaat cacccctctt gcaggtcttg cagtctgcaa ggcaatcaga
     6601 gaattcacgg gacttgactg ccgtataaaa tggccgaacg atatcatcat cggcaagaaa
     6661 aagcttgtcg gtattttaac cgaaatgagt gccgaatttg acgccgtaga atatgtaatt
     6721 acaggcatcg gaatcaatgc cgatactcag gattttcccg atgacataaa ggaaaaggca
     6781 acttcaattt atcttgcaac aggcggtcat tttgacaaga acaaattctt tgcctgtgtg
     6841 cttaaccaca ttgaagagga attcatcaaa aacgatttaa agctttcccc tgtcgcatta
     6901 aaggaataca caagcctttg cgcaacagtc ggcagagata taacattcac caaagacgga
     6961 aagtccgtca ccgcaaaagc tgtgggagta aaccctgacg gcgagcttat tgcagagctt
     7021 cccgacaaaa ccaccaaact tatcaattca ggcgaagtca ccgtacaagg catttattaa
     7081 cggcgtgtcg ccgtaccccg ttcgtgcaga cagttttaca aatgcaaagc tataaa
//
LOCUS       DAOMVE010000100         7122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2798_length_7122_cov_4.099335, whole genome shotgun sequence.
ACCESSION   DAOMVE010000100 DAOMVE010000000
VERSION     DAOMVE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2798_length_7122_cov_4.099335"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLS64_06530"
     CDS             complement(<1..624)
                     /locus_tag="PLS64_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRL43511.1"
                     /translation="MDKNNGAIYDARKLGKPKMIILGVQHMFAMFGATILVPILTGLD
                     ISTTLLMAGLGTLLFHCITKFKVPAFLGSSFAFLGGYAAIKAFSPNDPNSMLPYACLG
                     VACAGLIYFILAAVIKAVGIEKVMRFFPPVVTGPIIIAIGLGLAPSAVSNCTTNWFLA
                     VVALAVIVVFNIWGKGMAKIIPIILGLLISYGTGLVLYFISQANPDLI"
     gene            818..1444
                     /gene="upp"
                     /locus_tag="PLS64_06535"
     CDS             818..1444
                     /gene="upp"
                     /locus_tag="PLS64_06535"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRL43512.1"
                     /translation="MSVSYMTHPLIRHKITLLRKEDTSTNEFRKLVEEIAMLMGYEAL
                     SDLETQDITVKTPIEESVQPVISGKKLAVVPILRAGLGMVNGILALVPSARVGHIGLY
                     RDPKTHEPHEYYCKLPDPIDQRQIVVVDPMLATGGSAIAAVDFIKKHGGQKIKFMSII
                     AAPEGVDALMKAHPDIQLYVGCLDRELNENAYICPGLGDAGDRIFGTK"
     gene            complement(1749..2483)
                     /locus_tag="PLS64_06540"
     CDS             complement(1749..2483)
                     /locus_tag="PLS64_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRL43513.1"
                     /translation="MILHCTICDDNPSAVKREKEIAERAFCEMNISADISVYTDSSQF
                     MFELGDNKQMDILILDIEMPKMSGFEIADYVKKSNPNCLIIFMTSHVNYAVDGYKLDI
                     FRFVPKQDGDYRLKDALLDAANVIDLESKKSYLIERHDMCGMLPCKYILYIIKRGKNS
                     EIYHLKSKEPIKIRKPLSVVFEELDSNEFIYISRGCIANMENVEKVDRREWVCKNGDR
                     VPISASLYAEIKEKLLDFWGNKIIHD"
     gene            complement(2665..4203)
                     /locus_tag="PLS64_06545"
     CDS             complement(2665..4203)
                     /locus_tag="PLS64_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRL43514.1"
                     /translation="MKKNTKILIIVCAAALILAGLMCLLIFLPKGDGSSSGAATYDEG
                     VKMSVTTDKDGVHQAQIQTNDKGEIDNNSYGTLMDYIPAKISKIHLENKKGTLDIKSY
                     TPTDKNGKTSATQYTIVGYEDFDLQGGIADNIANNAASIDFTKVMTLDGSKLADYGLD
                     KPRDTVTVTYTDKTKAIIYVGDDAPQNAGTYIKFGSNDTVYLVAKDSVSAFDYGLTDL
                     ISLTINDAASDNDNSQASSIEISGSNFSNTITLKPNSDNKNSASYVMTSPVECYANEK
                     ESSLVAGGIRGLYALTVKMVNPSSSQLSSVGLSNPYAKLKAVYPDTTVSLRASKPDGD
                     GNVCLMKEGGNIVYTISAEKVPWVKTSYDSLVNEYVLYPKMSALSEVSVNDGKNTYTF
                     SLSTAQKTKTDDNGSESTTTTTTVKNGKTEIELATFSGFYENLTMVELADTKSDSKNG
                     SPVLTVTYKYSSDGSTDTVSYYKSDGNRYVAVVNGRVAGHAYQSKVNTAVKQASSVAA
                     NKSE"
     gene            complement(4203..5810)
                     /locus_tag="PLS64_06550"
     CDS             complement(4203..5810)
                     /locus_tag="PLS64_06550"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HRL43515.1"
                     /translation="MSEENKNLNTISEDTANADSNAVAETADKKEDTKAKKNKKNKSE
                     KGRFKKFMKSRKAKHGTVAMAITALVIVMVIIVNIIVGLLVNRFPDLELDLTSNKSFA
                     LQDDTIDYVSHLDKDVTVNILMAKESFESQGTYFVQAQKMLNKMASKSDGKMKIKYVD
                     LTSNPSFTSDYPNVDWQSSSANNIVLVESGKQYKVLTLTDCFEYDEKTYNYYGSYSFT
                     GTKIEQAVVTAILNVTTDDKVVVDMIKGNNEQDYSSLKTLLENNAYQVNEVSLATGDF
                     DSNAKIAIMYAPSVDLDEKIVEKLSTWLSNDGKYGRSLIYVPTADMGEMPNLDDFLKE
                     WGMSIDRGYVFETDESTLVSASSPYAFTVSYGDYYKDNLKNSKIPVVVSESHAVNITD
                     ENTAHALLKTSDKAGVLPIDADKNWDYKDAVSGNGENVAAEGVMTNEDKNSSRVIIFG
                     SYVMFSDTIMKYNSFNNSAYFMNVINTIADKEDVGITIESKSIDNTELGITDVATQNT
                     MLVVFVIIIPIAILVAGFVFWLRRRNR"
     gene            complement(5824..6528)
                     /locus_tag="PLS64_06555"
     CDS             complement(5824..6528)
                     /locus_tag="PLS64_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL43516.1"
                     /translation="MGAILKRELSSYFNSAIAYVVLAAYFFFSGVFFYNYCIVYNSAS
                     LSYVYGNMLLIILFIIPIMTMKSFADEKYQRTDQALFTAPVSLTRVVLGKFLGAFVLY
                     AICSAIFLLYAVVISFFATPEWSVILCTFIGLLLFGAALLAINIFISSLTESTIVAAI
                     VSMAVNLVIYMMTNFTSMISIDWIKNIVEKIDFATYYNSFKYGLLSAPDIVFFLSVAG
                     LFVFFTVRVLEKRRWS"
     gene            complement(6528..>7122)
                     /locus_tag="PLS64_06560"
     CDS             complement(6528..>7122)
                     /locus_tag="PLS64_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL43517.1"
                     /translation="SSAERSLSSGPGEQRRQRVGLAQALLGNPPVIILDEPTVGLDPK
                     QIIEIRKLIRNLGKKHTVIFSSHVLSEVSEVCDRIVVISKGKIVADAKTDELSAQVSG
                     GQKLSLTAEGSTADITEAIKKIPAVKRVTKTGSNADGSGKYMIEYETSSDIRRDVYNA
                     MVRVGAVILDMQSGNETLEDMFLKLTSGKYDSRKEDM"
BASE COUNT         2026 a         1536 c         1414 g         2146 t
ORIGIN      
        1 aataaggtcg ggatttgcct gtgaaatgaa gtaaagcaca agacctgtac cgtaggagat
       61 gagcaagccg agtataatcg gaatgatttt agccattccc ttgccccaga tgttgaaaac
      121 aacaattaca gcaagagcaa ctacagccaa gaaccagttt gttgtgcagt ttgaaacagc
      181 cgacggagca agtccaagac cgattgcaat gatgatagga cctgttacaa caggcgggaa
      241 aaatctcatt accttttcaa tgcccaccgc cttgataact gcggcaagga tgaagtagat
      301 aagacctgca cacgcaacgc caaggcacgc atacgggagc atactgttag ggtcattggg
      361 tgaaaaagcc ttgattgcgg cataaccgcc caagaatgca aaggacgagc cgaggaaggc
      421 aggtacttta aactttgtaa tgcagtggaa caaaagtgtt cccaaacctg ccataagcaa
      481 agttgttgaa atgtcaagtc ccgttaaaat cggaacaagg attgttgcgc cgaacattgc
      541 aaacatatgc tgaacaccga gaattatcat cttcggtttt ccgagtttgc gggcatcata
      601 aattgcgccg ttgtttttgt ccattagaat tccctctttc tcatgttcgg gtttaaaatc
      661 tgtaaaaacc cattcctgtt aattatacct ttacacagat ataaaagcaa taacttttta
      721 ttttaatgcc aaaagttccc atatcattga cattttgaac ggtttttagt atcattaagt
      781 taatcaaata atcaaaattc aacaaggagg caatgatatg tcagtttcgt acatgacaca
      841 tccgcttata agacacaaaa ttacacttct cagaaaagaa gacacatcaa caaacgaatt
      901 ccgcaaattg gttgaagaaa ttgcaatgct tatgggttac gaggctcttt cggatcttga
      961 aactcaggac atcacagtta aaactccgat tgaggagtct gttcagcctg ttatttcggg
     1021 caaaaagctt gccgttgttc cgattcttcg tgcaggtctt ggtatggtaa acggtattct
     1081 cgcactcgtt ccgtctgcaa gagtaggtca catcggtctt taccgtgacc ccaaaactca
     1141 cgaaccgcac gaatattact gcaagctccc cgatccgatt gatcagcgtc agattgttgt
     1201 tgtcgatccg atgcttgcaa caggcggttc ggctattgcc gctgttgatt tcatcaaaaa
     1261 gcacggcggt cagaaaatca agttcatgtc aatcatcgca gctcccgagg gtgttgacgc
     1321 tcttatgaag gcgcaccccg atattcagct ttatgtcggc tgtcttgacc gtgagcttaa
     1381 cgaaaacgca tacatttgcc ccggtttggg cgatgcaggc gacagaattt tcggcacaaa
     1441 ataatttatt ttccgcattc aaaaagcctg agcaaaaatg ctcaggcttt ttctgtttcg
     1501 cataaatgta aatgcagcag tgttaaaagt gtttcggggt taggatgtgc gaaacaggat
     1561 aaggttttcc aaaataacat atctttcgga ataactttca aagaacaagg tctgtgtatt
     1621 tatttgtgac cggttaaatt cacataccct gttttcgata ttattatagc acaaaaaata
     1681 tgtttgtgtt aattagtctt taaattgccg atttttgacc gctaatttaa tactttagtc
     1741 aacacgcatc aatcatgaat gattttattt ccccagaaat caaggagctt ctccttaatt
     1801 tccgcataga gtgatgcgct tatcggcact ctgtcgccgt ttttgcacac ccattcccgg
     1861 cggtcaactt tctccacatt ctccatattt gctatacagc ctctggaaat ataaataaat
     1921 tcatttgagt caagttcttc aaacacaaca gacagcggtt ttcgtatttt tatcggctct
     1981 ttgcttttga ggtggtaaat ttcactgttt ttacctcttt tgataatgta cagaatgtac
     2041 ttacacggca acataccgca catatcgtgc cgttcaatca gatacgattt tttgctttca
     2101 aggtcaatga cattagctgc gtcaagcaat gcgtccttca gcctataatc tccgtcttgc
     2161 tttggaacaa atctgaaaat atcaagttta tatccgtcaa cggcataatt tacatgggag
     2221 gtcataaaaa taataagaca attcgggttt gattttttca cataatccgc aatctcaaaa
     2281 cccgacattt ttggcatttc gatatcaaga atcagaatat ccatttgttt gttgtcgcca
     2341 agctcaaaca taaactgact gctgtcggtg tataccgaaa tatccgccga tatattcatt
     2401 tcgcaaaaag cacgttcggc tatttccttt tcccgtttaa ccgccgaagg gttatcatca
     2461 catattgtac aatgcaaaat catattcata acattcaccg tttaaagtta atataaatca
     2521 cttctacaaa atcattatac tatagaaatt atcgcattgc aatatattgt ctgaatttta
     2581 taaaacttgt gcctgtatat accagacaaa agaaacaccc gaactaaaaa gttcgggtgt
     2641 aaaagaataa atttaactca aagattattc cgatttattt gcggcaacac ttgaagcctg
     2701 tttgacagca gtattgactt ttgactgata tgcatgacct gcaactctgc cgttgacaac
     2761 cgcaacataa cggttgccgt cagacttgta atagcttacg gtgtctgttg agccgtcaga
     2821 tgagtattta tatgtaacgg taagaacagg cgaaccgttt ttgctgtcag actttgtgtc
     2881 ggcaagctcg accattgtaa gattctcata gaaacccgag aatgttgcaa gctcaatttc
     2941 tgtcttgccg ttcttaacgg ttgttgtggt tgttgttgac tcactgccgt tatcgtctgt
     3001 ctttgttttc tgtgcagtgc tcagcgagaa tgtgtaggta ttcttgccgt catttacgga
     3061 aacctccgac aatgcgctca tcttcggata aagcacatat tcgtttacaa ggctgtcata
     3121 gcttgtttta acccacggaa ccttttcggc agaaattgta tatacaatat ttccgccctc
     3181 tttcataagg cacacattgc cgtcaccgtc aggctttgaa gctctgaggc tgactgttgt
     3241 gtcgggataa acagccttta actttgcata cggattagaa agacctacgc ttgaaagctg
     3301 tgaggatgac ggattaacca tttttaccgt gagggcataa agacctctga tgccgcctgc
     3361 aacaagactg ctttctttct cgtttgcgta gcactcaaca gggcttgtca taacataaga
     3421 agctgagttc ttattgtctg aatttggctt gagtgtgatt gtatttgaaa aattagaacc
     3481 tgaaatctca attgacgatg cctgactgtt gtcattatca gatgccgcat cgttgattgt
     3541 aagactgata agatctgtaa ggccgtagtc aaatgcagaa acagaatctt ttgcaacaag
     3601 ataaacagta tcgttgctgc cgaacttaat atatgttccg gcattctgcg gagcatcatc
     3661 accgacatag ataatagcct ttgtcttgtc ggtataagtt acggtaacag tatctctcgg
     3721 cttatcaagt ccgtaatctg caagctttga gccgtcaaga gtcataacct ttgtaaagtc
     3781 aatgcttgcg gcattgtttg cgatattgtc ggcaatacca ccctgaaggt caaaatcttc
     3841 ataacctaca atagtgtact gtgtagcact tgttttgccg tttttatcgg tcggtgtata
     3901 tgacttgatg tccaaagtac cctttttgtt ttcaagatga atctttgaaa tctttgcagg
     3961 gatataatcc ataagagtgc cgtagctgtt gttatcaatt tcacccttat cgtttgtctg
     4021 aatctgtgcc tgatgaacac cgtctttatc ggttgtaacg ctcatcttta caccctcatc
     4081 atatgttgct gctcccgatg acgaaccgtc gcccttcgga agaaagatga gcagacacat
     4141 aagtcccgca agaatcaatg ctgctgcgca gacaataatc aatattttag tattcttttt
     4201 cattatctgt tcctcctgcg taaccagaaa acaaagcctg caacaagtat tgcaatcggg
     4261 ataatgatta caaacacaac aagcattgtg ttctgtgttg caacatcggt gattccgagt
     4321 tccgtattgt caatcgactt actctcaatt gtaatgccga catcttcctt atctgcaatt
     4381 gtgttgatta cattcataaa gtaagcggag ttattaaagc tgttgtactt cataattgtg
     4441 tcgctgaaca taacatatga accgaatatg ataactctcg atgagttctt gtcctcgttt
     4501 gtcataacac cttctgccgc aacattttca ccgttacccg aaactgcgtc cttgtagtcc
     4561 cagttcttgt cggcgtcaat cggcaatacg cctgccttat cggaagtttt aagcaatgcg
     4621 tgtgcagtgt tttcatctgt aatattaaca gcgtgtgact ctgatacaac aacaggaatc
     4681 ttgctgtttt tgaggttatc tttgtagtaa tcaccgtagc ttaccgtgaa tgcatacggt
     4741 gagcttgcgc ttacaagggt gctttcatca gtttcaaaca catatcctct gtcaattgac
     4801 ataccccatt ccttgaggaa atcgtcaagg ttcggcatct cgcccatatc ggcagtagga
     4861 acatagatga gtgagcgtcc gtatttgccg tcatttgaaa gccatgtcga aagcttttca
     4921 acaatcttct cgtcaaggtc aacagacggt gcgtacataa ttgcaatctt tgcgttgctg
     4981 tcaaagtcgc ctgttgcgag tgaaacctcg ttcacctgat aagcattgtt ttcaagaagt
     5041 gttttaagtg atgaataatc ctgctcgttg ttgcccttaa tcatatcaac aacaaccttg
     5101 tcatctgttg taacattgag aatagccgtt acaacagcct gctcaatctt tgtgccggta
     5161 aagctgtatg aaccgtagta attgtaggtc ttctcgtcat attcaaagca atccgtaaga
     5221 gtaagcacct tgtactgctt gccggattca acaagaacaa tgttatttgc cgagcttgac
     5281 tgccagtcaa cattcggata atcggatgtg aacgacgggt tggaggtaag gtcaacatac
     5341 ttgattttca tcttgccgtc tgacttgctc gccattttgt taagcatttt ctgagcctgt
     5401 acaaagtagg taccctggct ctcaaatgat tcctttgcca taagtatatt taccgtaaca
     5461 tccttgtcaa ggtgtgaaac atagtcaatt gtatcgtcct gaagtgcaaa cgacttgttt
     5521 gatgtaaggt caagctccag gtcggggaat ctgttaacaa gaagtccgac aataatgttt
     5581 acaataatta ccataacgat aacaagcgcc gtaattgcca ttgcaaccgt gccgtgtttt
     5641 gcttttcttg acttcataaa ctttttgaat ctgccctttt cggacttatt cttcttattc
     5701 tttttggcct ttgtatcttc ctttttatcg gctgtttccg ccacagcatt gctgtctgca
     5761 tttgcagtat cttcggagat agtattcagg tttttatttt cttcgctcat ttatattacc
     5821 tccttagctc cagcgtcttt tttccagaac acgaacagta aaaaatacga acaatcctgc
     5881 cacactcagg aagaatacga tatcaggtgc gctgagcaga ccgtatttga atgaattata
     5941 atatgtagca aaatcaatct tttcaacaat gttcttgatc cagtcaattg aaatcatact
     6001 tgtgaagttt gtcatcatat agataacaag gttaaccgcc atgcttacga ttgcggcaac
     6061 aattgtgctt tccgtaagtg atgaaatgaa aatgttaatt gcaagcagag ccgcaccaaa
     6121 gaggagaagt ccgatgaatg tacagagaat aaccgaccat tccggagttg caaagaatga
     6181 aattacaacc gcatagagaa ggaagattgc actgcaaatt gcataaagca caaatgcacc
     6241 gaggaacttt cctaaaacga cccttgtaag gcttacaggt gcagtaaaga gcgcctgatc
     6301 tgttctctga tacttttcat ctgcaaaaga tttcattgtc ataatcggaa ttataaacag
     6361 aataatcaaa agcatattgc cgtatacata gcttaaactt gccgagttgt acacaataca
     6421 gtagttgtag aaaaacacac ccgaaaagaa gaagtaagcg gcaagaacaa catatgcaat
     6481 tgccgaattg aaataagagg aaagctccct ctttaaaata gcacccatta catatcttcc
     6541 tttctgctgt catatttacc tgaggtaagc ttgaggaaca tatcctcaag tgtttcattg
     6601 cctgactgca tatcaagaat taccgcaccg actcttacca tggcattgta aacatctctt
     6661 ctgatgtcgc ttgatgtttc gtattcaatc atatactttc cgctgccgtc ggcattactg
     6721 cctgtctttg taacacgctt aacagcaggg attttcttta ttgcctcggt aatgtcagcg
     6781 gtacttccct ctgcagtaag cgagagcttc tgaccgcccg aaacctgagc agaaagttca
     6841 tctgtttttg catcggcaac aattttgccc tttgaaataa cgacgattct gtcacacacc
     6901 tcggaaacct cggaaagaac atgagatgaa aaaattacag tatgcttttt gccgagattg
     6961 cggataagct ttctgatttc gataatctgc ttaggatcaa gacctactgt cggctcgtca
     7021 agaatgatta cgggcggatt gccgagaagc gcctgagcaa gacctacacg ctggcgacgc
     7081 tgttcacccg ggccggagga aagtgaacgc tcagcgctgg ag
//
LOCUS       DAOMVE010000101         7036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2849_length_7036_cov_2.699183, whole genome shotgun sequence.
ACCESSION   DAOMVE010000101 DAOMVE010000000
VERSION     DAOMVE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_7036_cov_2.699183"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1477
                     /gene="rseP"
                     /locus_tag="PLS64_06565"
     CDS             92..1477
                     /gene="rseP"
                     /locus_tag="PLS64_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRL43518.1"
                     /translation="MQILTVAALIVIGILLFELIIFSHEFGHFITAKLSGVKVNEFAL
                     GMGPKIISFVKGETRYSLRLFPIGGYCAMEGEDEDSEEKGAFNNAKVWKRMIIIIAGA
                     VMNILLGFVMMFAFTVQADSYSSTTISQFQPNAFTANTGLQTGDKIVEVNGYSIWNSR
                     DLQFAISTLPYETVEGNTLEVYKERATSAACGVYNEYAKDESLSKDELQKYYNALSEG
                     CSKINKAASKDEAKKLLDETCKSIYALDKSYDMSKYKTPEIDTTTSRPRFMGDVKVVR
                     DGKEITLENVQFFTYYADEDAEKENKPTVAFDFAVEPIEKNVGTVLGETFTQTCSMAK
                     TVWTSLVWLVQGRFTFNDMSGPVGIATAVTQVASMGLQTGFGDAVNNILFVMILITVN
                     LGIVNMLPFPALDGGRFLFLLIEWIFKKPIPRKAEQIVNTVGLVLLLAFMLIISVKDV
                     FQLVTGTFPGM"
     gene            1496..2539
                     /gene="ispG"
                     /locus_tag="PLS64_06570"
                     /gene_synonym="gcpE"
     CDS             1496..2539
                     /gene="ispG"
                     /locus_tag="PLS64_06570"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRL43519.1"
                     /translation="MGSKIQVTAGNVKIGGGAKVSVQSMLNIPSTDIEGSVRQAKELE
                     EAGCEIIRAAIPNKDAVKLIPALKEAVSVPIVADIHFDYKLALEACAAGIDKIRINPG
                     NIGSDDRVKAVADACRQRGIPIRIGVNSGSLEKEILAKYGHPTPEALCDSALYHASLL
                     EKFDFNDIVLSMKSSTVSTMIKAYELAAERCDYPLHLGVTEAGTERMGIIKSSAGIGA
                     LLLHGIGDTIRVSLTADPVKEVYAAHDILKALDIEKDGVQFVSCPTCGRTRIDLVKIA
                     NEVEDKLRNCKKNIKVAVMGCVVNGPGEAREADIGIAGGDGCGLVFKKGEILRKVPED
                     KLVDALLEEVEKL"
     gene            2590..6948
                     /locus_tag="PLS64_06575"
     CDS             2590..6948
                     /locus_tag="PLS64_06575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HRL43520.1"
                     /translation="MKTLGEVFDSQFGIKLPQQISQGELTQLNINKQARLITLAVKFN
                     GLVERNTLFDTEKLITKTLAYHTVIKPHFPTELFSADYFPQLYAAVRRDIPSINGTLN
                     NAEVRFENNTLTINLLNGGKTLLDSKGFDKALIKLISEEFNLCISVNYTGTFEVEENS
                     EEYKAAIQDAQEKINRENLQKAAEFYQEEVETAEKREEKHAENTTVEIEVREGKFATP
                     QIIQSSIRPLYGRSIRGKMIPISSISGDSGRIVVWGDVFDIEKKVTKSGDKNIFTIDI
                     TDYTGSTTAKVFNSIKESAVIDNIKKGDTIVVQGDDEYDKYAGELVVNARSIGTAQKV
                     KVVDNAEKKRVELHMHTNMSQMDAVTSAGDLVNRAYQWGHKAVAITDHGVAQAFPDAM
                     KAADKINKDEEKIKIIYGVEAYFMDDLVESVKGDADTGFDGTFICFDIETTGLSAARD
                     KITEIGAVKVENGVITDTFSTFANPEMPIPQKITQLTGITDDMVKDAPSQSEAVGAFL
                     EFAGDNVLVAHNAPFDTSFIAKACEDMGREYNYTSIDTVAISRAILTDIKNCKLDTVA
                     KFLRLGDFNHHRATDDAEMLARIFINLCQRLTDDYGITKTNDINTKIAGGDFKKLPTY
                     HQIILVKNKTGLKNLYRLISYSHLNYFYKKPRIPKSELVKYREGLIIGSACCAGQLYM
                     AILEGKPWGELKQIASFYDYLEIQPAGNNSFMIRGGRFNSVDELHEIDRTIIKLAKEL
                     GKPVCATCDVHFMDPTDSEFRKILMAGQGFKDAEQQAPLYFRTTAEMLKEFEWLGKDK
                     AYEYVVENPNKIADMCEYIRPIPKGTFPPNIEGAQEQLIDITWKRAKEKYGDPLPEIV
                     KARLDKELNSITTYGFSVLYMTAQKLVADSEAHGYLVGSRGSVGSSFVATMSGISEVN
                     PLCPHYVCPNCKHSEFITDGSYGSGFDMPPKNCPECGTLMDQDGHEIPFETFLGFKGD
                     KVPDIDLNFSGEYQSKSHRYTEELFGKNNVFKAGTISTVAEKTALGFVKKFAQERGLV
                     MHKAEEKRLAIGCTGVKRTTGQHPGGMVVVPRTNDVYDFCPVQHPANDVNSDNITTHF
                     DFHSIHDTITKLDELGHDVPTIYHYLELYTGIPVMKVSMSDPEVMSLFTSPKALGVTE
                     EDIDSKTGTFSLPECGTAFVRGMLIEAQPKTFTDLLQIAGLSHGTDVWLGNAQELIHN
                     GSCTISEVIGTRDSIMTYLMHKGLEPGMAFKIMEIVRKGNATKLLTEEHFKAMREHNV
                     PEWYIDSCMKIKYMFPKAHAAAYMIATLRLGWYKVHKPVEYYAAYFTVRSENLDGAIA
                     MQGHQAVRDKMNNIKQKQSVHEATAKDEAEFQTLQIVNEMMARKIEFLPVDIYKSEAK
                     MFKVEDGKIRLPFSSLPGVGGAAADSLAETGKHTEYLSIEDMQIKTKVTKAVIETLKD
                     VGVLKDLPESSQMSLF"
BASE COUNT         2202 a         1395 c         1554 g         1885 t
ORIGIN      
        1 gagtatgtac tctctaagct ataatttgcg gtaatatttt ccactgccgt ctttgcgacg
       61 gcaatgaata ttttggagtt gatttatttt aatgcaaatt ttaacagttg cggctctcat
      121 tgtaatagga atattacttt ttgagcttat aattttttct cacgaattcg gtcattttat
      181 taccgcaaag ctttcgggtg taaaggtaaa tgaatttgca ctcggaatgg gaccgaaaat
      241 aatcagtttt gttaaaggcg aaacaaggta ctcgcttcgt ctttttccta taggcggtta
      301 ctgtgccatg gaaggagagg atgaagacag tgaagaaaaa ggtgctttta ataacgcaaa
      361 agtttggaag agaatgatta tcataatcgc aggtgcggtt atgaatattc ttctcggctt
      421 tgttatgatg tttgccttta ccgttcaggc tgacagctac agttcaacta caatatctca
      481 gtttcaaccc aatgcattta ctgcaaacac aggtttacaa acaggggata agattgttga
      541 agtgaacggt tattcgattt ggaattcacg agatttgcag tttgcaatct caactcttcc
      601 atatgaaaca gtagaaggca atacacttga ggtatacaaa gaaagagcaa catctgccgc
      661 ttgcggtgta tataatgagt atgcaaaaga cgaatctctt tcaaaggatg aacttcaaaa
      721 atactacaat gctctttctg agggttgttc aaaaataaat aaggctgcaa gcaaagacga
      781 ggctaaaaaa cttcttgatg aaacttgcaa gtcaatttat gcgcttgaca aatcatatga
      841 tatgtcaaag tataaaacac ccgaaataga cacaactaca tcaagaccga gattcatggg
      901 tgatgtaaaa gttgttcgtg acggcaaaga aataacactt gaaaatgtac agtttttcac
      961 ctattacgct gatgaagatg ccgaaaagga aaacaaacct actgttgcgt ttgactttgc
     1021 agttgaaccg attgagaaaa atgtcggtac tgttctcggt gaaacattca cacagacctg
     1081 ttcaatggca aaaaccgtgt ggacatctct ggtgtggctt gttcagggca gatttacatt
     1141 caacgatatg tcaggtcctg ttggtattgc tacagctgtc actcaggttg catcaatggg
     1201 acttcaaaca ggcttcggag atgcagtcaa taacattctt tttgtaatga tactcattac
     1261 ggtaaacctc ggaattgtaa atatgctgcc gttcccggcg cttgacggcg gaagattctt
     1321 atttttgctg attgaatgga tattcaaaaa gccgattccg agaaaggcag aacagattgt
     1381 gaatacagtc ggtcttgttc tgttgcttgc atttatgctt attatttcgg tgaaagatgt
     1441 gttccagctt gtaacgggaa cattccccgg aatgtagaca aacggaggaa attttatggg
     1501 aagtaaaatt caggtaactg ccggcaatgt aaaaatcggc ggcggtgcta aagtaagcgt
     1561 tcagtcaatg ctcaacattc cctcaaccga tattgagggt tctgtaagac aggcaaaaga
     1621 gcttgaagag gccggttgcg aaattattcg tgcagctatt cctaataaag atgcagtaaa
     1681 gcttattcct gccctcaaag aggctgtaag cgtcccgatt gtagccgata tccattttga
     1741 ctataagctt gcgcttgagg cgtgtgcggc aggcatcgac aaaatccgta tcaatccggg
     1801 taacatcgga tcagatgata gggtaaaggc ggtggcagac gcttgccgac agcgtggcat
     1861 tccgattaga atcggtgtta actcaggttc gcttgaaaag gaaattcttg caaaatacgg
     1921 tcatcccaca cccgaggcac tttgcgacag cgccttgtac catgcatcac ttcttgaaaa
     1981 gtttgatttc aacgatattg ttctttcaat gaaaagttca accgtttcta ctatgattaa
     2041 agcctatgag cttgccgcag aaaggtgtga ctatccgctt cacctcggtg ttacagaggc
     2101 gggaacagaa agaatgggta ttatcaaatc ttctgccgga atcggtgcat tactcctcca
     2161 tggtatcggc gatacaatca gagtttcact cacagccgat cctgtaaagg aagtttatgc
     2221 cgctcatgat attctcaagg ctcttgacat tgagaaagac ggagttcaat ttgtttcatg
     2281 tccgacttgc ggaagaacaa gaattgacct tgtgaagatt gcaaacgagg ttgaagataa
     2341 actcagaaac tgtaaaaaga atatcaaagt agcggtaatg ggttgcgttg taaatggtcc
     2401 cggagaggca agagaagctg atatcggcat cgcaggcggt gacggttgcg gtcttgtatt
     2461 caaaaaaggc gaaattcttc gcaaagttcc ggaagataaa ctcgttgacg cacttttgga
     2521 agaagtagaa aaattataac tctatcagca gtcaaaagac atttgctgta atatgacaga
     2581 aggacatata tgaaaacatt aggagaagtc tttgactccc aattcggcat taaactgccg
     2641 caacagattt cacagggaga actgacacaa cttaatatta acaaacaagc ccgtttgatt
     2701 actttagcag taaaattcaa cgggctcgtt gagcgtaata cgctttttga cacggaaaag
     2761 ctgattacca agacacttgc gtatcatact gtaataaaac cgcattttcc gacagagttg
     2821 ttttccgctg attattttcc tcagctatac gcagccgtaa ggcgagatat tccgagtatt
     2881 aacggaactc tcaacaatgc tgaggttagg tttgaaaaca atacgcttac gataaatctt
     2941 cttaacggcg gaaaaactct ccttgacagt aaaggctttg acaaagcact tataaagctc
     3001 atttcggaag aattcaatct ttgcatttcc gttaattata ccggaacctt tgaggttgaa
     3061 gaaaacagcg aagaatacaa agccgctatt caggatgctc aggagaaaat caaccgagaa
     3121 aatcttcaga aagccgctga attctatcag gaagaggttg aaacagcgga aaaaagagaa
     3181 gaaaaacacg ctgaaaacac tactgtggaa attgaagtcc gtgagggcaa gtttgcaaca
     3241 ccgcagatta ttcagtcctc aatccgtccg ttatacggtc gttcaatcag gggaaaaatg
     3301 attcccattt cttccatctc aggtgattcg ggaagaattg tggtgtgggg cgatgtattt
     3361 gatattgaaa agaaagtcac taaaagcggt gacaaaaata ttttcactat cgacataacc
     3421 gattatacgg gttcaacaac agctaaagta ttcaattcaa tcaaggaatc agccgtaatt
     3481 gataatatta aaaagggtga tacaatcgtt gttcagggtg atgatgagta cgataaatat
     3541 gcaggcgaac ttgttgtaaa tgcccgttca atcggtacgg ctcaaaaagt aaaagtagtt
     3601 gataatgccg agaaaaagcg tgttgaactc catatgcata caaatatgtc gcagatggat
     3661 gccgtaacat cggcaggtga ccttgtaaac cgtgcttatc agtggggaca caaagccgtt
     3721 gcaattactg accacggtgt cgcacaggct tttcccgacg caatgaaagc cgcagataaa
     3781 atcaacaaag acgaagagaa aattaaaatc atctacggtg ttgaggcata ctttatggat
     3841 gaccttgtag agtctgtaaa aggtgatgcc gacacaggtt ttgacggcac attcatctgc
     3901 tttgatatag aaacaacagg tctttcagcc gcccgtgaca agattaccga aattggcgca
     3961 gtcaaggttg aaaacggtgt catcaccgat actttttcaa cctttgcaaa tcccgaaatg
     4021 ccgattccgc agaaaatcac tcagcttaca ggcattaccg atgatatggt aaaggacgct
     4081 ccgtcacaga gcgaggcggt tggtgcattt cttgaatttg ccggtgacaa tgtgcttgtt
     4141 gctcacaatg ctccctttga tacatccttc atagcaaaag cctgtgaaga tatgggcagg
     4201 gaatataact acacttcaat cgacacggtt gcaatttcaa gagcgattct tacagatata
     4261 aagaactgca agcttgatac ggttgcaaaa tttttaaggc tcggcgactt caatcaccac
     4321 agagcaaccg atgatgccga aatgcttgcg agaattttta taaatctctg tcagcgactt
     4381 acagatgatt acggtataac aaaaacaaac gacattaata caaagatagc aggcggagat
     4441 tttaaaaagc ttccgaccta tcatcagatt attcttgtaa agaataaaac aggtcttaaa
     4501 aatctttaca gacttatttc ttacagtcat cttaattatt tctataaaaa gccgagaatt
     4561 ccgaaaagcg agcttgtgaa gtacagagag ggactcataa tcggctcagc ttgttgtgca
     4621 ggtcagcttt atatggcaat tcttgagggc aagccgtggg gcgaactcaa acagattgcg
     4681 tcattctatg attatcttga aattcagcct gcaggcaaca acagctttat gattcgtggc
     4741 ggcagattta attctgttga cgaacttcat gaaattgacc gtacaataat taagcttgca
     4801 aaagaactcg gcaagcctgt atgcgcaacc tgcgatgttc actttatgga tccgacagat
     4861 tccgaattca gaaaaattct tatggcaggt cagggtttta aagatgccga acagcaagct
     4921 ccgctttatt tcagaacaac tgctgaaatg ctcaaggaat ttgaatggct cggcaaggat
     4981 aaagcctatg agtatgtagt agaaaatccc aataaaatcg ccgatatgtg cgaatatatc
     5041 agacctattc caaagggtac attcccgcct aacattgagg gcgcacagga acagcttatt
     5101 gatattacat ggaaaagagc aaaagagaaa tacggtgatc cattgcctga aatcgtaaag
     5161 gcaagacttg acaaggaact taattcaatc acaacctacg gattctctgt actttatatg
     5221 actgctcaaa agcttgttgc agacagtgag gcacacggat atcttgtcgg ttcacgagga
     5281 tcggtaggtt cgtctttcgt agctactatg tccggtattt cggaggttaa tccgctttgt
     5341 ccgcactatg tatgtccaaa ctgcaaacac agcgagttta taacagacgg cagttacggc
     5401 tcaggctttg atatgccccc aaaaaactgt cctgaatgcg gtactcttat ggatcaggac
     5461 ggtcacgaaa ttccgtttga aacatttctt ggctttaaag gcgacaaagt gcctgatatc
     5521 gaccttaact tcagcggtga gtatcagtca aaatctcacc gttacacgga agagctcttc
     5581 ggtaagaata atgtgttcaa agcgggaaca atttctaccg tagccgaaaa aacagcactc
     5641 ggcttcgtta agaagtttgc acaggagcga ggtcttgtaa tgcacaaggc tgaggaaaag
     5701 cgtcttgcaa tcggttgtac gggtgttaag agaaccacag gtcagcaccc gggcggtatg
     5761 gttgttgtgc cgagaacaaa cgatgtttac gatttctgcc ctgttcagca tcctgcaaat
     5821 gatgtgaact cagataacat tacaacccac ttcgacttcc attcaattca tgatacaatc
     5881 acaaaacttg atgagctcgg acacgatgtt ccgacaatct atcattatct tgaactctac
     5941 acaggtatcc ccgtaatgaa agtttcaatg agcgatcccg aggttatgtc gctgtttact
     6001 tctccgaaag cactcggagt tactgaggag gatatcgact caaaaaccgg tacattcagc
     6061 cttcctgaat gcggtacggc tttcgtaaga ggaatgctca ttgaggcaca gcccaaaacc
     6121 tttaccgacc ttttgcagat tgcaggtctt tcgcacggta ccgatgtatg gctcggaaac
     6181 gctcaggaac ttattcataa cggatcctgt acaatttcgg aagtaatcgg tactcgtgac
     6241 tcgattatga cttatcttat gcacaaaggt cttgaaccgg gcatggcatt caaaataatg
     6301 gagattgtcc gtaaaggtaa cgcaacaaaa cttcttacgg aagaacactt caaggctatg
     6361 cgtgaacaca atgttcccga atggtatatt gattcatgta tgaaaattaa gtatatgttc
     6421 ccgaaagccc atgccgcagc ctatatgatt gcaacgctca gactcggttg gtacaaggtt
     6481 cacaagcccg ttgaatatta tgcggcatac tttaccgtac gaagtgaaaa tcttgacggt
     6541 gcaattgcca tgcagggaca tcaggctgtc cgtgacaaga tgaacaacat aaaacaaaaa
     6601 cagagtgttc acgaagccac agccaaggat gaagcagagt tccagacctt gcagattgtc
     6661 aacgaaatga tggcaagaaa aattgaattt cttcctgttg atatctacaa atccgaggct
     6721 aaaatgttca aggtagagga cggaaaaatc agacttccgt tttcatcctt acccggtgta
     6781 ggcggcgccg ccgcagattc actcgcagaa acgggcaagc acacagaata tctttcaatt
     6841 gaagatatgc agataaaaac aaaggttact aaggctgtta ttgaaacact gaaagatgtc
     6901 ggagttctca aggatttgcc tgaaagctca cagatgtcat tattttaagg agttgtctaa
     6961 atgaaaaaac tgctttcgct aaaaactttg ctgatttgca tttcatttac agtatttctt
     7021 ttacttgtaa aagata
//
LOCUS       DAOMVE010000102         7000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2878_length_7000_cov_3.930310, whole genome shotgun sequence.
ACCESSION   DAOMVE010000102 DAOMVE010000000
VERSION     DAOMVE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2878_length_7000_cov_3.930310"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1381
                     /locus_tag="PLS64_06580"
     CDS             17..1381
                     /locus_tag="PLS64_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRL43521.1"
                     /translation="MTNSEYSDVTPQIHALAQKMTHNSVIDTDLYSKYNVKRGLRDLD
                     GKGVLTGLTDISTITQNKLVDGKLVPCEGELRYRGYNVKDIVDGILEDDRFGFEEVTY
                     LLLFGDMPNEEQLKDFKKLLVQYRTLPQHFVRDVILKATSNDMMNSIARSVLTLFCYD
                     KNANDTSIDNVLRQCIQLISVFPLLSVYGYHAYNHYQRDNSLYIHRAEPTMSTAEVIL
                     SLLRPDRHYTPLEAKVLDMALILHMEHGGGNNSTFTTHVVTSSGTDTYSAVAAALASL
                     KGPKHGGANVKVYYMFEDLKKHVKDWEDEDALEDYLEKLLDKQVFDKKGLIYGMGHAV
                     YTISDPRQQILHGAVKKLALAEGHNDEFDLYERVERLAPKVIGRKRKIYKGVAANVDF
                     YSGLLYSLLDIPCELYTPLFATARITGWSAHRLEELINCGKIIRPAYMSISENKEYVD
                     LKDR"
     gene            complement(1668..2108)
                     /locus_tag="PLS64_06585"
     CDS             complement(1668..2108)
                     /locus_tag="PLS64_06585"
                     /inference="COORDINATES: protein motif:HMM:NF040112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HRL43522.1"
                     /translation="MATTKKTTAKTAPAKTAPVKEEPVKKTVAKAEPAKTVAAAAKKA
                     EPTKTENVKVETVKKAEPVKKAPVKKAAPKKVAPKKPVPVETKVNFFVEYAGAQVPVE
                     EVIKNVKATYGKDAKEISIYLKPEESKAYFVADGNAEEMDVFFC"
     gene            2406..3344
                     /locus_tag="PLS64_06590"
     CDS             2406..3344
                     /locus_tag="PLS64_06590"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL43523.1"
                     /translation="MGENFIIIAQQVLVLFILIAVGFICGKKKIITDFSARHMTDIVL
                     YVVTPCVMISAFQREFSFELLSGLIICVACSALIFAVSILICNLIFHDKDESRKAVIR
                     FATIYSNCAFMSLPLQKAILGDDGWFYGSIFVAVFNIFVWTHGLVSMSGDKKQLSFKK
                     LVLNPGLIGVLLAIILFITGFKLPYIISQPVEYLAVLNTPLPMLIIGFYLSQADFKKA
                     FTDKGVYFSSAVRLIALPMITAVVMSLCRVTPIITMACVIATSAPTAATTTMFATKFN
                     KDVELSVSIVAATTVFSLATMPLVVMLTGMMSGFSS"
     gene            3741..4742
                     /locus_tag="PLS64_06595"
     CDS             3741..4742
                     /locus_tag="PLS64_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HRL43524.1"
                     /translation="MSRNPMQKQKLLYLRKILLEKTDENHGLTSSEILAELALYGITA
                     ERKSLYDDLQVLEKFGMDICKSKSSTVKYYVASREFELPELKLLVDAIQSSKFITEKK
                     SISLIHKLEGLASVHDGKELQRQVFVANRAKTMNERIYYSVDKIQNAIALNRKISFKY
                     YRWELDFSGTERIVKREKIRDGGYTASPWALCWDDENYYLIAYDTCADSIKHYRVDKM
                     DDIEVLDDERDGGKTFEKFDPADYSKGVFSMFGGEKATVKLSVDNDCIGVIVDRFGRD
                     IFVTKESDTTFGVSVDIMTSKQFYAWVFGLGGKVRIISPQNVVDEFKKQLENVNDSF"
     gene            5056..5916
                     /locus_tag="PLS64_06600"
     CDS             5056..5916
                     /locus_tag="PLS64_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL43525.1"
                     /translation="MDFDFLRSATASSSAYRVVCGEMIIDSNMWNVHNEVLVAVVRDG
                     YMTVEMGADTYSLSTGDIYFISPNQRYKINNTGNARVDVILLNLSNPASLTQEFIPQS
                     LIRGLVTGNCTHFAKVTRGDYRYNNLIDDMNTVISAENEKHEFFQILVHGKMYDIFYI
                     LFSSGLVKICDVEAQGKKYRALRRITEYINNNFCDSITLDTIAQTTGLSRYYVSHLFK
                     ELMNTTFVNYLNELRLTRAAMLLTTTDTPVIEIAGMSGFNNISNFNRAFKMYYETTPS
                     KYRRAEKQSF"
     gene            6135..6926
                     /locus_tag="PLS64_06605"
     CDS             6135..6926
                     /locus_tag="PLS64_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43526.1"
                     /translation="MKFFNKNINKVLSVVLVVVSVLVVLQFVCSNGTLNAGETPTESA
                     MARVNPNIRKIVPTTTVSATTVPVETTTAKPKATKATTQPQTKATKATKATTQPVTTT
                     QATVPFATAIPPKEPVDYQSQWDAGYLVAIDNPDKTYECSKVTLTDEDRDLLERLCMG
                     EFGSGGFIGAALIAQSVKDAMCFDGYPTVASVIENCHYTGSTKIGTNQECIQAVSYIF
                     DENKDAVQHRIMYMYNPDMVQSAFHESQNYILTYQTVRFFDRWGY"
BASE COUNT         2047 a         1361 c         1476 g         2116 t
ORIGIN      
        1 aaaaggagtg ttttttatga caaacagcga gtattcggat gttacaccgc agattcatgc
       61 tctggctcag aaaatgacgc ataactccgt tattgataca gatttataca gtaagtacaa
      121 tgtaaaaaga ggtctgcgtg accttgacgg taagggtgtt ctcacaggtc ttacagatat
      181 ttctacaatc acacagaata agcttgttga cggaaagctt gttccttgcg agggtgagct
      241 tcgttacagg ggctataatg taaaggatat cgttgacggt attcttgagg atgaccgttt
      301 cggttttgaa gaggttacat atcttcttct ctttggcgat atgcctaacg aagaacagct
      361 taaagatttt aagaagcttc tcgttcagta cagaaccctt cctcagcact ttgttcgtga
      421 tgttatcctc aaggccacaa gtaacgatat gatgaactca atagcaagaa gtgttctcac
      481 acttttctgc tatgataaga acgctaacga tacatcaatc gacaatgttt taagacagtg
      541 tatacagctt atctccgtat tccctctttt gtcggtttac ggctatcacg catataatca
      601 ctatcagcgt gataacagcc tttatatcca ccgtgcagag cctacaatgt caacagccga
      661 ggttattctg tcgcttcttc gtcccgacag acactatact ccgcttgagg caaaggttct
      721 tgatatggcg ctcattctcc atatggaaca cggcggcggt aacaactcaa catttacaac
      781 acatgttgta acatcttcgg gtactgatac atactcggca gtagctgcgg cacttgcatc
      841 actcaaaggt cctaagcacg gcggtgcaaa tgttaaggtt tactatatgt ttgaggatct
      901 caaaaagcat gttaaggact gggaggacga ggacgctctt gaagattatc ttgagaagct
      961 ccttgacaag caggtttttg ataaaaaagg tcttatctac ggtatgggac acgcagttta
     1021 tacaatttcc gacccccgtc agcagattct tcacggtgct gttaaaaagc ttgcgcttgc
     1081 cgaaggtcac aatgatgaat ttgatcttta tgagagagtt gaaagactgg ctccgaaggt
     1141 tatcggcaga aagcgtaaaa tctataaggg tgttgccgca aatgtcgatt tctacagcgg
     1201 acttctttac agccttcttg atattccgtg tgagctttat actccgcttt ttgcaacagc
     1261 tcgtattacg ggttggagcg cacacagact tgaggagctt atcaactgcg gtaagattat
     1321 ccgtcctgca tatatgagca tttccgaaaa caaggaatat gttgatttga aagacagata
     1381 atacggcgaa ccgccgttcc ccgttcgaga agttagtttt acaagtgcga gggttaaatt
     1441 gcccgagcgt atggaaaatt gaaagttaaa aatcaaagtc ggacgacagt ttgcaaaatg
     1501 caaatcattg tcgcccgacc gttttttata tatgaaatta actgtttggt cgtggggcga
     1561 ataatatccg cactacgatt tttcgcaaca ttcatcttaa aacaacaaaa gaccgctctt
     1621 ttgagcggtc tttaaagctt gtgttttaaa ttcaaaattt attgcaatca gcagaagaat
     1681 acatccattt cttctgcgtt gccgtcggca acaaagtatg ccttgctttc ttcgggcttg
     1741 agatagattg aaatttcctt tgcatccttg ccgtatgtag ccttaacatt tttgattact
     1801 tcttcaacag gaacctgtgc gcctgcatat tctacgaaga aattaacctt tgtttcaacc
     1861 ggaacgggct tctttggagc aaccttcttt ggagctgctt tcttaacagg agccttttta
     1921 acgggttctg ctttcttaac agtttcaacc tttacatttt ctgtctttgt gggttcagcc
     1981 ttctttgcag ctgcagcaac agtcttagca ggctcagcct ttgcaacagt cttttttaca
     2041 ggctcttcct taacgggagc agtttttgca ggggctgttt ttgcagttgt tttctttgtt
     2101 gttgccataa taaatccttc tttcaaagta ataaaatatt gcagaaataa aagttttatc
     2161 ttttgtaaat attatacatt tcagagtaaa aaatgtcaat aaactgtaca aaaattcggc
     2221 ggttaataca tatttgcgaa aaaataaaat tcctgttagt taaaatgcac aatgttatta
     2281 actcatattt gtgaactatg tcgttttttc tgtcaaaaaa aacgaaaaaa agcacatcat
     2341 catttgaaaa cctcttgcaa ctgtgctaaa atgatagcga ttaatttaag agagaagaat
     2401 gatttatggg agagaatttc attattattg cacaacaggt gcttgtactg ttcattttga
     2461 ttgcagtcgg ttttatttgc ggtaagaaga aaattatcac cgatttttcc gcccgtcaca
     2521 tgaccgatat agtattgtat gttgtgacgc cctgcgttat gatttctgct tttcagcggg
     2581 agttctcgtt tgaacttttg agcggactta taatttgcgt tgcctgctct gcacttattt
     2641 tcgctgtgtc aatacttatc tgtaacctga tttttcatga taaagatgaa agccgtaagg
     2701 ctgttatccg ctttgcaaca atctattcaa actgtgcatt tatgtctttg cccctccaga
     2761 aggcaattct cggtgatgac ggttggtttt acggctcaat tttcgttgcc gtattcaaca
     2821 tttttgtatg gacgcacgga cttgtttcaa tgagcggtga caaaaaacag ctttcattca
     2881 aaaagcttgt tctcaacccc ggccttatcg gtgtccttct tgcaattatc ctgtttatta
     2941 caggttttaa gcttccgtac ataatcagtc aaccggttga atatcttgca gttctgaaca
     3001 ctccgctccc gatgcttatt atcggttttt acctttcaca ggctgacttt aaaaaggcgt
     3061 tcactgacaa gggcgtatat ttttcttcgg cagtccgtct gattgcttta ccgatgatta
     3121 cagcggttgt aatgtcattg tgccgtgtca cacccataat taccatggct tgcgtaattg
     3181 ccacatcagc ccctactgcc gcaacaacaa ctatgtttgc aacaaagttc aacaaggatg
     3241 ttgagctgtc ggttagtatc gttgccgcaa ctacggtatt ttcacttgca acaatgccgc
     3301 ttgtcgtaat gcttacgggc atgatgagcg gatttagtag ttaatgtaaa atggaaaatt
     3361 gaaaattatg gtcggcggtg tgccaccgca gacctttcga gaagatagtt gagataaatg
     3421 aattgcattg atgcccgaaa cggcattgat gcaattttta ttttatatga atttaactgt
     3481 ttaattgcag aggaaaaccg tggcctcccg ttagtaatgg cagaaaattt ctgttctcga
     3541 attgggaacg gcggctcgct atgtgggagg attaaataaa caattacaat tctacgctcg
     3601 ggcatgatat gtttttgata ttacaaacta tctacacgaa ttgggaacgg cggctcgccg
     3661 taaaaatata tggaaatctc ggctgaaatt tgatataatg aacacataaa aatatataat
     3721 cctttgaggg ggactgtatt atgtcgagaa atcctatgca aaaacagaaa cttttgtatc
     3781 tgcggaaaat tcttcttgaa aagaccgatg aaaatcacgg gctgacttca agtgaaattc
     3841 tggcagagct tgcgttgtac ggaattactg cggagcgaaa aagtctttac gatgatttgc
     3901 aggtgcttga aaaattcgga atggacatct gcaagagcaa atcatcaacc gttaaatatt
     3961 atgttgcaag cagagaattt gaactgcccg agttgaagct tcttgttgac gcaattcaaa
     4021 gctcaaaatt cattactgaa aaaaagagca tttccctcat tcataagctt gaagggcttg
     4081 caagtgttca cgacggcaag gagctgcaac ggcaggtttt tgtcgcaaac agagccaaaa
     4141 caatgaatga acgaatttat tattcggttg acaaaattca gaatgcaata gcactcaacc
     4201 gcaaaatctc gttcaaatac taccgttggg aactcgattt ttcgggaacg gagcgaattg
     4261 tcaagcgtga aaaaatcagg gacggcggat acacggcttc accgtgggcg ctgtgctggg
     4321 atgatgagaa ttactatctc attgcctatg acacctgtgc cgacagtata aagcattacc
     4381 gtgttgacaa aatggacgat atcgaggtgc ttgacgatga gcgtgacggg ggaaagacct
     4441 ttgaaaaatt tgatcctgca gattattcaa agggcgtttt ctcaatgttc ggcggtgaaa
     4501 aagctacggt taagctgtcg gttgacaatg actgcatagg tgttattgtt gacaggttcg
     4561 gcagggatat ttttgttaca aaggaaagcg acacaacatt cggtgtatcg gttgatatta
     4621 tgacaagcaa acagttctac gcttgggtat tcggtcttgg cggaaaggtc agaattatat
     4681 caccgcaaaa tgttgttgat gaatttaaaa agcagcttga aaatgtaaac gattcatttt
     4741 aattgccata cggttggcaa tatttcatat taaaatcgtt aaataataat gtctgattcg
     4801 gaaacaatca taacaattat cagtagaaaa attgatacat tattgcgtag aattaaaaat
     4861 caattaacat acagcagaca gcggaatatt taaaatttgc actaattgca gtttatacat
     4921 ttattttttg cacatttgtc gtaattattg actttattgt gcataaagtc tattatattg
     4981 ttgcagtgat tatttgtgtt gtttttagac acaaaatgtt tgaaaacata attttttaga
     5041 atcggggtta ttattatgga ttttgatttc ttgcgttcgg caaccgcatc cagttcagct
     5101 tacagagtag tttgcggtga aatgattatt gacagcaata tgtggaatgt tcacaacgag
     5161 gttcttgtgg ctgttgtaag ggacggctat atgactgttg aaatgggtgc tgacacttat
     5221 tcgctctcaa caggcgacat ttactttatt tcacctaatc agagatacaa aattaataat
     5281 acaggcaacg caagagttga cgttattctt cttaaccttt caaatcctgc atcgctcact
     5341 caggagttta ttccgcagag tctgatcaga ggtcttgtaa caggcaactg cactcacttt
     5401 gcaaaggtta cgagaggcga ctaccgttat aacaatctta ttgacgatat gaacacggtt
     5461 atttctgccg agaacgaaaa gcacgagttc ttccagattc ttgttcacgg caaaatgtac
     5521 gacattttct atattctttt ctcatcgggc cttgttaaga tttgtgatgt tgaggctcag
     5581 ggcaaaaagt accgtgctct ccgcagaatt acggaatata tcaacaacaa cttctgcgac
     5641 agcattactc ttgatacaat agctcagaca acaggtctca gccgttacta tgtgtcgcat
     5701 ctctttaagg agcttatgaa tacaacattt gtaaactacc ttaacgagct cagactgaca
     5761 agagccgcaa tgcttcttac tacaacggac actcccgtta tcgaaattgc aggtatgtcg
     5821 ggcttcaaca acatttcaaa ctttaacaga gcattcaaaa tgtactacga aacaacacct
     5881 tcaaagtaca gaagagccga gaaacagtca ttttaaataa gtattttcaa aataaaaagc
     5941 cggtgcatat gcgtcggctt ttgtttatgg taataatttg tccgcttagg taatattgtt
     6001 taaaaaaggc agttcatttt tgtagaatgc ttaaattgaa aagaaaagca taccgtggta
     6061 taattattat atgtgtgaca gcgtgtcttt ttgtgcgtca tttgtgttta gaagtattaa
     6121 acggcggtga aacgatgaag tttttcaata aaaatataaa caaggttttg tcggttgtgc
     6181 ttgttgtagt atccgttttg gtagttttgc agttcgtctg ctcaaacggt actctcaatg
     6241 ccggagaaac tccgacagaa tcggcaatgg caagggtaaa tccgaatatt cggaagattg
     6301 ttccgacaac aactgtttcc gcaacaaccg tacccgttga aacaacaacg gcaaagccta
     6361 aagcaaccaa ggcaacaact cagcctcaga caaaagccac aaaggcaaca aaagcgacaa
     6421 ctcagcccgt gacaacaaca caggcaaccg ttccgtttgc tacggcaatt ccgccgaaag
     6481 agcctgttga ttaccaatct caatgggacg caggttatct tgtggcaatt gacaatccgg
     6541 acaaaaccta tgaatgctcc aaggtaacgc ttaccgatga ggacagggat cttcttgaaa
     6601 ggctttgcat gggcgaattc ggttcgggcg gttttatagg agcggcgctc attgctcagt
     6661 cggtaaaaga tgcaatgtgc tttgacggtt atccgacagt tgcctcggtt atcgagaact
     6721 gccattatac cggcagtacc aaaatcggca caaatcagga atgtattcag gcggtaagct
     6781 atatctttga cgaaaacaag gatgccgttc agcaccgcat tatgtatatg tataatcccg
     6841 atatggttca gagcgctttc cacgaaagtc agaactatat cctgacatat cagactgtca
     6901 gattttttga caggtggggg tattgacgaa aatctttgat tttcgtcagc taaattcacg
     6961 caagcgtgag ctaaatttgc tacgcaagct aaattatcct
//
LOCUS       DAOMVE010000103         6992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2885_length_6992_cov_3.450483, whole genome shotgun sequence.
ACCESSION   DAOMVE010000103 DAOMVE010000000
VERSION     DAOMVE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2885_length_6992_cov_3.450483"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..154)
                     /locus_tag="PLS64_06610"
     tRNA            complement(78..154)
                     /locus_tag="PLS64_06610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(118..120),aa:Arg,seq:ccg)
     gene            complement(335..2248)
                     /locus_tag="PLS64_06615"
     CDS             complement(335..2248)
                     /locus_tag="PLS64_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRL43527.1"
                     /translation="MPALSVRNLTMTFIERNLFTDVSFDVEERDKVGFIGANGVGKTT
                     LFKILNGEISPVSGTVTFSKNVRPGYMEQHACNNPRADVYHELLSVFDHLSDMETEIS
                     ALAHQIDNKSGNLDELVERQTMLIEQFERAGGLTYKSRTRSALLGLGFSENDFTMPVG
                     NLSGGQRSKLCLAKLLLSQSNMLLLDEPTNHLDIDAIAWLEGFLRDFKGAMIIISHDR
                     YFLDNVTNKTIELEHNRAMVYKGSYSEFVKKKESVNESLKNKYEHDLKEIKRIEGIVE
                     QQKRWGQAHNFITAASKQKEADRIKDGLVAPESELETMRMHFEPRCESGNDVLICKNL
                     AKSFDDKQLFKNVDIHIRKGERVFIIGGNGCGKTTLFRILTGKTPMNSGEYDYGANVE
                     IGYFDQMQQNLDLSKTALDEVWDTFPNMTQTEVRSALASFLFKGDEVFKPLSKMSGGE
                     RARVSLLKLMLKGSNFLLLDEPTNHLDASSREELEKTLLDYSGTMLIVSHDRYFINKI
                     ADRILLLTNNGVKEYLGNYDYYLERTTAEKSGAVPTENKKDKKEKTQNDYFLQKQKQS
                     EERKRQTKLKKAEAEIERLDEEIAKTQELLSSEEVAADYEKLMELSKLLEDLQKQQEE
                     QYEIWEELESMAE"
     gene            complement(2290..2712)
                     /locus_tag="PLS64_06620"
     CDS             complement(2290..2712)
                     /locus_tag="PLS64_06620"
                     /inference="COORDINATES: protein motif:HMM:NF039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HRL43528.1"
                     /translation="MTEKTGLEKTVVFNLKSANESDVNNIVNEKGHESRSFYYKLGLL
                     YFLNIVDWICTEALIGSGHFVEANPVMQPVLENFWLTLLIKGILPLVLILGCALIYKL
                     AEIGRSKTAEMLLTVGIVSYALVNLWHIFNFVLLFCRF"
     gene            complement(2805..3101)
                     /locus_tag="PLS64_06625"
     CDS             complement(2805..3101)
                     /locus_tag="PLS64_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRL43529.1"
                     /translation="MADTKNEIMNEEDQGMLITLEDENGQEVEFEFLDVIEFEGTEYI
                     VLIENDEDADEVVILQINQIDEETEEYVSIDDEDTLNKVFELFKKKYEGDINFE"
     gene            complement(3312..3578)
                     /locus_tag="PLS64_06630"
     CDS             complement(3312..3578)
                     /locus_tag="PLS64_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43530.1"
                     /translation="MTPILSLHNVDVTKFLAVLDTCEGNVYLVTSEGDRLNLRSKLCQ
                     LVGLTRLIEGGRIAEASIICENKNDESKLFRLNMFGDTGNAKVG"
     gene            4117..5619
                     /locus_tag="PLS64_06635"
     CDS             4117..5619
                     /locus_tag="PLS64_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRL43531.1"
                     /translation="MLNDFYKQFKSGTDIRGVASEGVEGQSVNLTDDVVADMADGFVL
                     WLSKKVSKKPSELKISVGRDSRISGPHIMKITTERFKRCGAEVLCCQLASTPSMFMTT
                     VDLGCDGALQITASHHPFNRNGLKFFTREGGLEGSDIEEILLYAQNGEHPEESNGGNI
                     TDVDYMSDYAKGLCEKIKKEVNAEDYEHPLKGFKIVVDAGNGAGGFYADKVLSVLGAD
                     TTGSRYLEPDGMFPNHVPNPEAKEAMDSICEAVRESGADLGVIFDTDVDRGGAVDSKG
                     NEINRNRLVAVAAAIALEGNDGGMIVTDSITSSGLKQFIENDLGGKHYRYRRGYKNVI
                     DKALELNAQGINCPLAIETSGHAAMRENYFLDDGAYLCTKIIIKAAQMRKEGKELDEL
                     TASLKEPLESTEIRYKILEKDFRACGEKIIADLTKYAEEQDGWCVADDNREGVRVSFD
                     RDNGDGWFLLRLSVHDPIMPLNVESDSKGGVKIICDKLGEFLKTTTGLDL"
     gene            5784..6626
                     /locus_tag="PLS64_06640"
     CDS             5784..6626
                     /locus_tag="PLS64_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL43532.1"
                     /translation="MNIETANRLLQYRKKMNLSQEELAARIGVSRQAVSKWERAEASP
                     DTDNLILLADIYGVSLDELLKGEKETESETQSEEPENNAENNPDSDTTYKKSDKVSFK
                     HGIHVDSKDGDSVHLNFRDGIHVKDKDGTKVDVGWNGIHVEENGKTKVYTDENGNVMY
                     DESIEEHKHSKSHRFWKHFPFWLIAVIGFLVWGFSGVLLGWVLSWVCFLTIPLYYTLV
                     DAIYKRKPSHFAYPLLVVIVYMFFGFLDICGGWSFGWIAFLTIPIYYEICKLFKSERK
                     SSDD"
     gene            complement(6782..>6992)
                     /locus_tag="PLS64_06645"
     CDS             complement(6782..>6992)
                     /locus_tag="PLS64_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRL43533.1"
                     /translation="RYVYLPENMTFVKLSGTSVTTEKMSKGTHYIEVALNEVPLFIKD
                     GRQIELCKPAMRTSQLDTTDTVMIP"
BASE COUNT         1982 a         1467 c         1438 g         2105 t
ORIGIN      
        1 ttttctttac ggtcggcttc aaatcccttc ggattctaaa cagaccaaaa aaagacaccc
       61 cgtagggtgt ccttttttgg cctgcccgaa gggatttgaa cccccattct ccggaatcgg
      121 aatccgttgc gttatccaat tgcgccacgg gcagatgtat gcggcagggt attttgcctg
      181 ccgtttttca tttttaattc gcataatctt tcatctgaaa atcaactgca aaaactgctg
      241 tgcaacagtc tttagcttat attatgcggt tattatacaa caatttttta ctttaagcaa
      301 gggaaatttg tcgctcctcc cgactgtaag gcgatcattc tgccatactt tcgagttctt
      361 cccagatttc gtactgctcc tcctgctgtt tttgcaaatc ctcgagcagt tttgaaagct
      421 ccataagttt ttcgtaatct gcggcaactt cctcacttga gagaagctcc tgtgttttgg
      481 cgatttcttc gtcaagtcgc tcaatttcag cctctgcctt tttgagctta gtctgtcttt
      541 tgcgttcttc actctgcttt tgcttttgca aaaagtaatc attctgcgtt ttttcttttt
      601 tatctttctt attctcagtc ggaacagcac ccgatttttc ggctgttgtg cgttcaagat
      661 aataatcgta attaccgaga tattccttta ctccgttatt tgtaaggagt aaaattctgt
      721 cggcaatttt atttataaaa taacggtcat ggctgacaat cagcattgta ccgctgtagt
      781 caagcaaagt tttttcaagt tcttcccgtg atgatgcgtc aaggtggttt gtcggctcat
      841 cgaggagcaa aaagttactg ccttttagca ttagtttcaa gagagaaact ctcgccctct
      901 cgccgccgct catttttgaa agcggtttaa acacttcgtc acctttgaac aggaatgacg
      961 caagcgcact tctgacttcg gtctgcgtca tattcgggaa ggtgtcccac acctcatcaa
     1021 gtgcggtttt tgacaggtca agattctgct gcatctgatc aaaataaccg atttcaacat
     1081 ttgcgccgta gtcatattca ccgctgttca taggagtttt gcccgttaaa attctgaaca
     1141 gcgttgtttt accgcaaccg ttgccgccga tgatgaacac tctctcgcct tttctgatat
     1201 gtatatcaac atttttgaaa agctgtttgt cgtcaaacga cttggcaagg tttttacaga
     1261 ttaaaacatc gttgccgctt tcacatctcg gctcaaaatg catacgcatt gtttcaagct
     1321 cgctttcggg ggcaacaagc ccgtctttga ttctgtcagc ctctttctgc ttacttgcgg
     1381 ctgtgataaa gttgtgtgcc tgtccccagc gtttttgctg ttcaacaatg ccctcaatac
     1441 gctttatctc ctttaaatcg tgttcatatt tatttttaag cgactcgttc accgattcct
     1501 ttttcttgac aaattccgaa tatgagccct tataaaccat agcacggttg tgttcaagtt
     1561 caatcgtctt gttggttaca ttatcaagaa aataacggtc atggctgatg ataatcattg
     1621 cgcctttgaa atctctcaaa aagccttcaa gccatgcgat tgcgtcaatg tcaaggtggt
     1681 ttgtcggctc gtcaaggagg agcatattgc tctgcgacag caacagcttt gcaaggcaga
     1741 gctttgaacg ctgaccgccg cttaaattgc cgacaggcat tgtaaagtca ttttcggaaa
     1801 atccgagtcc taaaagtgcg gaacgggttc tgcttttata ggtcagaccg cctgctcttt
     1861 caaactgttc aataagcatg gtctgccttt caacaagctc gtcaagattt cccgacttgt
     1921 tgtcaatctg atgtgcaagt gcggaaattt cggtttccat atccgaaaga tggtcgaaca
     1981 cggaaagcag ctcgtgatac acatctgccc tcggattgtt gcaggcatgc tgttccatat
     2041 atcccggacg gacatttttt gaaaaggtaa cagttcccga aacagggctg atttcaccgt
     2101 tgagtatctt aaagagagtt gttttaccga ctccgttagc tccgataaag cccaccttat
     2161 ctctttcttc aacatcaaac gaaacatcgg taaaaaggtt tctttcgata aaagtcattg
     2221 taagatttct tactgataat gccggcaaaa catacactcc ctttcaaatt cgctacccac
     2281 tattgtacat taaaatcgac aaaagagcaa tacaaaattg aaaatatgcc ataggtttac
     2341 aagggcataa gacacaattc cgaccgttaa aagcatttcg gctgttttgc tcctgccgat
     2401 ttcggcaagc ttgtaaatca atgcacaacc cagaataagc acaagcggca aaattccctt
     2461 gattaacaat gtcagccaaa agttttcaag cacaggctgc ataacgggat ttgcctcaac
     2521 aaaatgtccg ctgccgatga gcgcctctgt acaaatccag tcaacaatat tgagaaaata
     2581 gagtaagccg agtttgtaat aaaacgaacg gctttcgtga cctttttcat tgacgatatt
     2641 attgacatca gattcatttg ccgatttcaa attaaacaca acggtttttt caagtccggt
     2701 tttttctgtc atacacaaca cctcaacggt attttttccc gcagagaatg ttatattcac
     2761 acaaaagaaa agagcctccc ctcggaagct caatttgaaa cgaattattc aaagttaatg
     2821 tcgccctcat atttcttttt gaaaagctca aataccttgt taagagtatc ttcatcgtca
     2881 atgctgacat actcctctgt ttcttcgtca atctgattaa tctgaagaat aacaacctcg
     2941 tctgcatctt cgtcattttc gatgagaaca atgtactctg taccctcaaa ctcgattaca
     3001 tcaagaaatt caaattcaac ttcctgaccg ttttcgtcct caagagtgat gagcataccc
     3061 tgatcttctt cgttcataat ctcgtttttt gtatctgcca tagcgtattc ctttccgaat
     3121 gcgtttaacg cctcataaaa taaattaagt ttagtataac ccaaaacatc ggcaaaatca
     3181 acactttatt gatattttac atttaaaagg taaaagccaa aaacagctgc gattgctcgc
     3241 agctgtcctt gggtttagaa gttttgaaat taacgtgaaa accgtagata tttaagtcag
     3301 tgttttcaga attaacctac tttggcgtta cctgtgtcgc cgaacatatt aagtctgaaa
     3361 agcttgctct cgtcgttctt gttttcgcaa ataatacttg cttctgcaat cctgccgcct
     3421 tcgataagtc ttgtaagacc gacaagctgg caaagcttgc tgcggagatt gagtctgtcg
     3481 ccttcgcttg taacgagata tacattgccc tcgcaggtat cgagcacggc gaggaatttg
     3541 gttacatcaa cattgtgtaa gctaagaatt ggtgtcataa aatttcctcc attcaattct
     3601 ttaaaaatat aaagttttgt tttcgtgcgc catttaccat ttggcaaaat catgatcaat
     3661 ttatcgacta tgtgataatt caattttact cgagtaaggc taattggaaa tcagccgata
     3721 gtcaagcgga ctatttactt gacaattatt attatagtca aacattcgga aaagtcaaca
     3781 attttagcga ttaatttttg ttcacaaata cttttaggtg tcagttatgc acaattagtt
     3841 atcgtatttt ttgtacaata tgctttgtat ttgcttttat agaaatatag ttttaacaca
     3901 agcataacat ttgattgtgt aaattcaaca acttatttct ttttcagcat tatataactg
     3961 ctgtttttta caaacttaat ttaacaggca tattgagatt ttgctcattt taagttataa
     4021 tctatgttat gaaggctgat atcaaaaagg attcaggatt gctccgaatt aaattttcaa
     4081 taaaagcaat catacaaata agaaaggaat catgaaatgc ttaacgattt ttacaaacag
     4141 ttcaaaagcg gaacagacat acgaggagtt gcaagcgagg gtgttgaggg acaaagtgta
     4201 aaccttaccg acgatgttgt tgccgatatg gcagacggct ttgtgctttg gctttctaaa
     4261 aaagtttcaa aaaagccgag cgagctcaaa atctctgtcg gtcgtgacag ccgtatttcg
     4321 ggaccgcata taatgaaaat cacaaccgag cgttttaagc gttgcggtgc agaagttctc
     4381 tgctgtcagc ttgcgtcaac tccgtcaatg tttatgacaa ccgttgacct cggatgtgac
     4441 ggtgctcttc agattaccgc aagtcaccac ccgttcaaca gaaacggtct taaatttttc
     4501 acccgtgagg gcggtcttga gggcagtgac attgaggaaa ttcttctcta cgcacaaaac
     4561 ggtgaacatc ccgaagaaag caacggcgga aatattacgg atgttgacta tatgagcgac
     4621 tacgcaaagg gactttgcga gaagattaaa aaggaagtta atgcagaaga ttacgagcat
     4681 ccgctcaagg gctttaagat tgttgttgac gcaggcaacg gtgcaggcgg attttatgcc
     4741 gacaaggtgc tttctgttct cggagcagac acaacaggca gccgttatct tgaacctgac
     4801 ggaatgttcc cgaatcatgt gccgaatccc gaggctaaag aggcaatgga ttcaatctgt
     4861 gaggctgtca gagaatcggg cgcagacctt ggcgtaatct ttgatacgga tgttgaccgt
     4921 ggcggtgctg ttgacagcaa gggcaatgaa atcaatcgaa acagacttgt agccgttgcg
     4981 gcggcaattg ctcttgaggg caatgacggc ggtatgattg ttacagactc aatcacatca
     5041 agcggtctta aacaatttat tgaaaatgac ctcggcggaa agcattaccg ctatcgcaga
     5101 ggctataaga atgttatcga caaggctctt gaactcaacg ctcagggcat aaactgtccg
     5161 cttgcaatcg aaacatcggg acatgccgca atgcgtgaaa actactttct tgatgacggt
     5221 gcatatctct gcacaaaaat tattatcaag gccgctcaga tgagaaaaga gggcaaggag
     5281 cttgacgagc ttaccgcctc actcaaagaa ccgcttgaaa gcacagaaat cagatacaaa
     5341 attcttgaaa aagatttcag agcctgcggt gaaaaaatta ttgccgacct tacaaagtat
     5401 gccgaggagc aggacggctg gtgtgttgcc gatgataacc gtgagggcgt cagagtttcg
     5461 tttgacaggg ataacggtga cggctggttc ttgctcagac tttcggtaca cgatccgatt
     5521 atgcctctca atgttgaaag tgacagcaag ggcggtgtaa aaatcatctg cgacaagctt
     5581 ggtgaatttt taaagactac aacaggtctt gacctctgat attgggatat tttgcgtttt
     5641 tattgatttt tattgtcgca actacgggtt gcaaccgaga gttgcgacaa tggaacccga
     5701 aatgtattgt tttttggggc aggcaaccga tataattttc tcacaaggta aaaccctaaa
     5761 gcaatatttg aggaggaaat tttatgaaca ttgaaactgc caacagactt ttgcagtaca
     5821 gaaaaaagat gaatctaagc caggaggagc ttgccgcaag aataggtgta agccgtcagg
     5881 ctgtgtcaaa atgggagcgt gcagaggcat cgcccgacac ggataatctt attcttcttg
     5941 ctgatatcta cggtgtttcg cttgatgaat tgcttaaagg cgaaaaagaa accgaaagcg
     6001 aaacacagtc ggaagaaccc gaaaataatg ccgaaaataa tcccgacagc gatacaacat
     6061 acaaaaaatc agacaaggtt tcgttcaaac acggaatcca tgttgactca aaggacggtg
     6121 attctgttca ccttaatttc agggacggaa tccatgttaa agataaagac ggtacaaagg
     6181 ttgatgtcgg ctggaacgga atccatgttg aggaaaacgg caaaacaaaa gtctatacag
     6241 acgaaaacgg aaatgttatg tatgatgaaa gcattgaaga gcataaacat tcaaagtcgc
     6301 accgcttttg gaaacacttt ccgttttggt tgattgccgt tatcggattc ctcgtttggg
     6361 gattcagcgg agtattgctc ggttgggtac tcagctgggt atgctttctg acaattcctc
     6421 tctattacac acttgttgac gcaatctaca aacgcaagcc gagtcatttt gcttatcccc
     6481 tgcttgtcgt tattgtgtat atgtttttcg gatttctcga catctgcggc ggttggtcat
     6541 ttggttggat tgcattttta acaattccga tttattatga aatctgcaag cttttcaaaa
     6601 gcgaaagaaa aagttctgac gattaaaact gaattaaaga catacaaaaa cacggcagtt
     6661 ttttatcaac tgccgtgttt gattttgctg taaactatgt ccttgtataa agtgtaaacc
     6721 atgttgttga catagtattc gcccatagat tgtgcaaaca gataatcgta caaataaaat
     6781 tttacggaat cataactgtg tctgttgtgt cgagctgtga agttctcatt gcgggcttgc
     6841 agagttcaat ctgcctgccg tctttgataa agagcggaac ttcattgaga gcaacctcaa
     6901 tataatgagt acccttgctc attttttctg ttgtaacgct tgtaccgctg agttttacaa
     6961 aagtcatgtt ctcggggaga taaacatatc tt
//
LOCUS       DAOMVE010000104         6957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2903_length_6957_cov_3.895393, whole genome shotgun sequence.
ACCESSION   DAOMVE010000104 DAOMVE010000000
VERSION     DAOMVE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2903_length_6957_cov_3.895393"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..3009)
                     /gene="adhE"
                     /locus_tag="PLS64_06650"
                     /gene_synonym="adhC"
     CDS             complement(388..3009)
                     /gene="adhE"
                     /locus_tag="PLS64_06650"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HRL43534.1"
                     /translation="MSTKEYPIVDNAESFEAALARVREAQKIFATYTQEQVDKIFLAA
                     ATAANQMRIPLAKMAVEETGMGVAEDKVIKNHFASEYIYNAYRNVKTCGVLEEDKAYG
                     IKKIAEPIGVIAAVIPTTNPTSTAIFKTLVCLKTRNGIIISPHPRAKKSTIEAAKVVL
                     EAAVKAGAPEGIISWIDIPSLELTNTLMQEADCILATGGPGMVKAAYSSGKPALGVGA
                     GNTPAIIDETADILLAVNSIIHSKTFDNGMICASEQSVIVEKKIYSKVKKEFKARGCY
                     FLNEEETEKVRKTIIINGALNAKIVGQKPVTIAALAGVTIPEETKVLIGEVESVDISE
                     EFAHEKLSPVLAMYKAEDFEDAIAKAEHLIADGGYGHTSSLYVDAVNERAKIDEFASR
                     MKTCRILVNTPSSQGGIGDLYNFKLTPSLTLGCGSWGGNSVSENVGCKHLINIKTVAE
                     RRENMLWFRAPEKVYIKRGCMPVALQELKDVMGKKRAFIVTDSFLYKNGYTKPITDKL
                     DEMGITYTTFADVEPDPSLISAQKGAEAMRKFEPDCIIALGGGSAMDAGKIMWVLYEH
                     PEVDFQDMAMRFCDIRKRVYTFPKMGEKAYFIAIPTSSGTGSEVTPFAVITDQNTGVK
                     YPLADYQLMPKMAIVDANNMMSGPKGLTAASGIDAVSHALEAYASMMATDFTDGLALR
                     SLKLIFEYLPACYDNGMNEPVAREKVAHAATMAGMAFANAFLGVCHSMAHKLGAFHHV
                     PHGIANALMLEQVIRFNSAEVPAKMGTFPQYDHPHTLARYAEVARYLGLGGKNDTESV
                     ENLIKAVNDLKARVGIKNSIKDYVPDEEDFLNRLDAMTEQAFDDQCTGANPRYPLFKE
                     IKQMYLNAYYGNNSETV"
     gene            complement(3086..3877)
                     /locus_tag="PLS64_06655"
     CDS             complement(3086..3877)
                     /locus_tag="PLS64_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRL43535.1"
                     /translation="MKRILCFLMACTMCASAAVGLSGCGCDDNTSSNSSKPGYKVEPT
                     EPDLTNGDFGFFIINQEELMITKYTGSDTVIEIPESYKNYKVTVIGASVFNNSKITEV
                     TIPSSIKQIEDYAFSSCHSLTKVNLSEGLEILNNSVFFNCSELREIKLPSTLKEIGPS
                     AFSGAALNNVVLPDSGKLTKIDEYAFYQSRELTDITIPACITSIPDNVFAECSKEVTI
                     HGASGSYAQNYAKKNNLKFKADLGSSSTKATKASKASGKAAEKAE"
     gene            complement(4157..5293)
                     /locus_tag="PLS64_06660"
     CDS             complement(4157..5293)
                     /locus_tag="PLS64_06660"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRL43536.1"
                     /translation="MATAVICEFNPFHNGHKYLLESAKNVLKEPVIAIMSGSFTQRGE
                     VAVCDKFVRAKSALENGADLVLELPVVFSLSGAEGFAKAGVAIASAFDCTNHLAFGSE
                     SGDCELLKKSANAVCDERVQALVRDGMKNGGYYPRELENAVKTVFGDEISSVLCEPNN
                     ILGVEYIKALNGTGVEPFSVARTGVAHDSRIAGEGFASASHIREMLRNGENAKKYAPY
                     IPDEITFPENLDRPLLYRLRTMTREDIAKLPDVGEGLENRIADAAVRCSTSSEIADLV
                     KTKRYTHARIRRILACALLGIEKAHTQIPIEYVRVLGFTNEGAELLKDCRLNIVTSAS
                     NGIRTGGNTKALLEKDILAYDISALAYEIPKRSGLDFTTPIVKI"
     gene            5498..6706
                     /locus_tag="PLS64_06665"
     CDS             5498..6706
                     /locus_tag="PLS64_06665"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HRL43537.1"
                     /translation="MKILVINAGSSSLKYQLIDTDNEAVLAKGNCERIGQNGAFIGFK
                     TPDGKKINRKTQMLNHTQAFEAVKNALIDPEYGAISDLSEVSAVGHRVVQGGAYFDKS
                     VLVNNSVINKIRELAPLAPLHNPAHLQGIEACTRVFGPKVPQVAVFDTAFHQTMPEKA
                     FMYAVPYKYYEKFGVRKYGFHGTSHRYVSEKMADLMGRKKEELKLITCHIGNGSSITA
                     VKGGKVIDTTMGLTPLGGFMMGTRSGSLDPSVITFIAEKEHLTPEEMSKILNKESGLL
                     GISGVSSDDRDVCAAEADGNMRAHLAHEMLYYQIAKYIGSYYVALGGCDGIVFTAGIG
                     ENQPMLREKVCDYLECLGVKLDKEFNKQATCGVTGTLSTPDSAIRVELIATDEEMVIA
                     RDTRAIVEAL"
BASE COUNT         1883 a         1569 c         1487 g         2018 t
ORIGIN      
        1 atcatttatt gaaaacaaat tttataaatg tcacaaaatc attgatgttt tgtaagggag
       61 aaccgtgttc tccctttcgt gtgcaacgca cacgattgca agcaatgcgt gcaaatttga
      121 acacacagtt ttaatcacat caaaaaatat atgtataaac ataaatattc atcaagtcgg
      181 gcgcccaatg agcgccccta ctggtacatt tatataattt gttttaacat ataatttgta
      241 tatgttcgtt ttggttttgc aaccaaaacg attggcgaac acggttcgcc gctacagtat
      301 tacaaacaca ttgcaacatt attcatatac aacaaaacac cctgccacgg gggcagggtg
      361 ttttataatt atgcattacg agatagatta aacagtctcg ctgttgttgc catagtaagc
      421 attgaggtac atctgcttga tttccttgaa gagcggatat cttgggtttg cacctgtgca
      481 ctggtcatca aatgcctgct ctgtcatagc gtcaagacgg ttgaggaagt cctcttcgtc
      541 cggaacataa tccttgatag agttcttgat gccaactctt gccttgaggt cgttaacagc
      601 cttgataagg ttttcaacgc tctctgtatc gttcttgccg ccgaggccga ggtaacgagc
      661 tacttcagca taacgagcga gtgtgtgcgg gtgatcatac tgtgggaatg tacccatctt
      721 agcaggaact tctgctgagt tgaatctgat tacctgctca agcataagtg cgttagcaat
      781 accgtgcggt acatgatgga aagcaccgag cttatgagcc attgagtgac aaacaccgag
      841 gaatgcgtta gcgaatgcca tacctgccat tgtagcagcg tgagcaacct tctcacgagc
      901 aacaggctcg ttcataccgt tgtcatagca agcagggaga tactcgaaga taagcttgag
      961 tgaacggagt gcaagaccgt ctgtgaaatc tgtagccatc attgaagcgt aagcctcaag
     1021 tgcgtgagaa acagcgtcga tacctgatgc agctgtaagt cccttaggac cgctcatcat
     1081 gttgttagca tcaacgattg ccattttagg cataagctga tagtcagcaa gcggatactt
     1141 aacgcctgta ttctggtctg tgataactgc gaaaggagta acctcagaac ctgtacctga
     1201 tgatgttggg atagcaatga agtatgcctt ctcacccatc ttcgggaatg tgtaaactct
     1261 cttacggata tcgcagaaac gcatagccat atcctggaag tcaacttccg gatgctcata
     1321 gagaacccac ataatcttgc cggcatccat tgctgaaccg ccgccgagtg cgataatgca
     1381 gtcaggctca aacttacgca tagcctctgc acccttctga gctgaaataa gtgatgggtc
     1441 aggctcaaca tctgcgaatg ttgtatatgt gatgcccatt tcatcgagct tgtctgtgat
     1501 tggctttgtg tagccattct tataaaggaa tgagtcagta acgatgaatg ctctcttctt
     1561 acccattaca tccttgagtt cctgaagagc tacaggcata cagcccctct tgatataaac
     1621 cttttcaggt gctctgaacc aaagcatatt ttctctcctc tcggcaactg tcttaatatt
     1681 gataaggtgc ttgcaaccaa cattctcaga aacagagttg ccgccccatg aaccacagcc
     1741 gagtgtgagt gaaggagtaa gcttgaagtt gtaaaggtca ccgataccac cctgtgaaga
     1801 aggtgtgtta acaaggatac ggcaagtttt cattcttgat gcgaactcgt caatctttgc
     1861 tctttcgttt acagcgtcaa cataaagtga agatgtgtga ccgtaaccgc cgtcagcaat
     1921 aagatgctca gcctttgcaa tagcatcttc aaaatcttca gccttgtaca tagcgagtac
     1981 aggagaaagc ttctcgtgag caaactcttc gctgatgtca actgattcta cctcaccgat
     2041 aagaaccttt gtttcctcag gaattgttac acctgcaaga gctgcgattg taacaggctt
     2101 ctgaccaaca atcttagcgt taagagcgcc gttgatgatg atagtctttc taaccttctc
     2161 tgtctcttcc tcgttaagga agtagcagcc acgagccttg aattccttct taactttgct
     2221 gtaaatcttc ttttcaacga taacagactg ctctgaagca cagatcatac cgttatcaaa
     2281 tgtctttgag tggataattg agttaactgc gagtaaaatg tcagctgttt catcaatgat
     2341 agcaggtgtg ttaccggcgc caacaccgag tgcaggctta cctgatgaat aagcagcctt
     2401 aaccatgccg gggccgcctg ttgcgaggat gcagtcagct tcctgcataa gtgtgtttgt
     2461 gagttcgagt gaaggaatat caatccagct gatgataccc tccggagcgc ctgccttaac
     2521 agcagcttca agaacaacct tggcagcctc aattgtgctc ttcttagcac ggggatgcgg
     2581 gctgatgatg ataccgtttc ttgtcttaag acaaacgagt gtcttgaaga tagcggttga
     2641 agttgggttt gttgtaggaa taacggcagc gattacaccg attggctcag caatcttctt
     2701 aatgccgtaa gccttgtctt cctcaagaac gccgcaagtc ttaacatttc tgtaagcatt
     2761 gtaaatatac tcagatgcga agtggttctt gataacctta tcttctgcaa cgcccatgcc
     2821 tgtttcttca acagccatct tagcgagcgg aattctcatc tggttagcag cagtagcggc
     2881 agcaagaaaa atcttgtcaa cctgctcctg tgtgtaggta gcaaaaattt tctgagcttc
     2941 tcttactctt gcaagggcag cttcaaagct ttctgcgtta tctacgatag gatactcttt
     3001 tgtactcatt taatctacct cttatcttgc ataattatat tagattcagc aaagctgaaa
     3061 atgatatcaa attaatttgc ctcaattact cggctttctc ggcagcctta ccgcttgcct
     3121 ttgatgcctt tgttgccttt gttgaagaac tgcctaagtc agccttgaat ttaaggttgt
     3181 ttttcttagc atagttctgg gcatatgaac cgcttgctcc gtggattgta acttccttac
     3241 tgcattcagc gaatacattg tcgggaatac ttgtaataca agccggaatt gtaatgtctg
     3301 taagctcacg cgactgataa aaagcatatt catcaatctt tgtaagttta ccgctgtcgg
     3361 gaagaacaac attgttaagt gccgcacccg aaaaagcgct cggtccgatt tctttgagtg
     3421 tactcggaag cttgatttct cttaactcag agcagttaaa gaacacgctg ttgttaagga
     3481 tttcaagacc ttcggaaaga tttacctttg tgaggctgtg acagcttgaa aaggcataat
     3541 cttcaatctg cttgatactt gacggaattg ttacttccgt aattttggag ttattgaaca
     3601 cgcttgcacc gattacggta actttgtagt tcttgtagct ttcgggaatt tcaataaccg
     3661 tgtcagaacc tgtgtacttt gtaatcataa gttcttcctg attgataatg aaaaatccga
     3721 aatctccgtt tgtaaggtca ggctctgtcg gctcaacctt gtagccgggc ttactgctgt
     3781 ttgatgatgt gttgtcgtca caaccgcatc ccgagagtcc gaccgctgct gatgcgcaca
     3841 ttgtgcacgc catcagaaag caaagaattc ttttcatttt tctggatcct ttcaattgtt
     3901 gtttcagaat ttagctaatt ctcgattgat tctattttat catctttgtt aaatatttgt
     3961 caatgttttt ttgaccattt ttctactttt atcgggttca ataataatta caaaaagatt
     4021 acacacttat tgttgcactt ctcacaaaaa agataaaaat cccgtaaaat ccgctgtttt
     4081 ttgcggtaaa aaatgtaaga aaaatcctca gaaactgccg ttttcggcat atcattgacg
     4141 aatcaatcga ccaatgtcat attttcacaa taggcgttgt aaaatcaaga ccgctccttt
     4201 ttggaatttc atacgcaagt gccgaaatat cgtaggctaa aatgtccttt tcaagcaatg
     4261 cctttgtgtt gccgcctgtt ctgataccgt tgcttgcaga ggtgacaata ttcagtcggc
     4321 agtctttgag caattccgca ccctcgtttg taaagcctaa aacacggaca tactcaatcg
     4381 ggatttgcgt atgagctttt tcaattccga gcaatgcaca ggcgagtatt ctccgtatgc
     4441 gtgcatgagt gtagcgcttt gtctttacaa ggtcggcaat ttctgatgag gtactgcaac
     4501 gcactgccgc atcggcaatt ctgttttcaa gcccctcacc cacatcgggc agttttgcaa
     4561 tgtcctctct tgtcatagta cgcaggcggt acagtaacgg tctgtcaaga ttttcgggga
     4621 atgtgatttc atcgggaatg tacggagcgt acttcttcgc attttcaccg ttacgcagca
     4681 tttctcttat gtgtgacgca gacgcaaagc cctcgcccgc aattcggctg tcgtgtgcaa
     4741 cacctgttct tgcaaccgag aacggctcaa cacccgtacc gttcaatgcc tttatatact
     4801 caacgccaag aatattgtta ggttcgcaga gtacggacga aatctcatct ccgaaaaccg
     4861 ttttgacggc gttttcaagt tcacgagggt agtatccgcc gtttttcatt ccgtcacgca
     4921 caagagcctg taccctttca tcacaaacag cgtttgcgga ttttttgagc agttcgcaat
     4981 caccgctttc actgccgaag gcaagatgat ttgtgcagtc aaaagccgac gcaatcgcaa
     5041 cacctgcctt tgcaaagccc tcagcccccg aaagtgagaa cacaacgggc agttcaagca
     5101 ccaaatccgc accgttttca agggcagatt ttgcacggac aaatttgtcg cacacagcaa
     5161 cctcaccacg ctgggtaaag ctgccgctca taattgctat gaccggctct ttaagcacat
     5221 tttttgcact ttcgagcaaa tatttatgac cgttgtgaaa cggattgaat tcgcagatga
     5281 cagctgttgc cataactatt cctccgaagc ctttatttcg catacatatt gtaaaaaaca
     5341 cttgaaaaca tacataattt ggagtatcat atgtatgtgt gacctgtcaa ttttgaaaag
     5401 tctttttctg atttacatta tagtataaaa tatgacgaca ggcaaatata tttcttatat
     5461 tttaaactga aaacaactac caaaggggac gattgtaatg aaaattctcg taatcaacgc
     5521 aggcagttcc tcgctcaagt accagcttat tgatactgac aacgaggcag ttctcgcaaa
     5581 gggtaactgt gaaagaatcg gtcagaacgg tgctttcatt ggttttaaaa cacccgacgg
     5641 caaaaaaatc aaccgcaaaa cacagatgct taatcacact caggcttttg aggctgttaa
     5701 aaatgcactc atagatcccg aatacggtgc aatcagcgat ttgtccgagg tttccgccgt
     5761 aggtcacaga gttgtgcagg gcggtgcgta ctttgacaag tcggtgcttg taaataactc
     5821 ggtaatcaat aaaatcagag agcttgctcc tcttgcacct cttcacaacc ctgcacatct
     5881 tcagggcatt gaggcctgca caagagtttt cggtccgaaa gttccgcagg ttgcggtttt
     5941 tgatactgct tttcaccaga caatgcccga aaaagcattt atgtatgcag tgccttacaa
     6001 gtattatgaa aagttcggtg taagaaagta cggctttcac ggcacatctc accgttatgt
     6061 ttccgaaaaa atggctgacc ttatgggcag aaaaaaggaa gagctcaagc ttattacctg
     6121 tcacatcggc aacggttctt caatcacagc cgtaaagggc ggtaaggtta tcgacactac
     6181 aatgggactt actcctctcg gcggttttat gatgggtacc cgttcaggtt cacttgatcc
     6241 gtctgttatc acatttatcg ctgaaaaaga gcatctcact cccgaggaaa tgtcaaagat
     6301 tctcaacaag gaatcgggtc ttttgggcat ttcgggcgtt tcgtctgacg accgtgatgt
     6361 ttgtgcagcc gaggcagacg gcaatatgag agctcacctt gcccacgaaa tgctttacta
     6421 ccagattgca aagtacatcg gcagctatta tgtagcgctc ggcggttgtg acggcatcgt
     6481 attcacggca ggtatcggtg aaaaccagcc tatgctccgt gaaaaggttt gcgactatct
     6541 tgaatgcctt ggcgttaagt tggataagga attcaacaag caggctacat gcggtgttac
     6601 aggcacactc tcaactcctg attctgcaat cagagttgaa ctcattgcaa ccgacgaaga
     6661 aatggttatt gcaagagata caagagccat tgttgaggca ctttaattga aataaatctg
     6721 ccacgcagta aatattacat aagaaaacag gttgtcatat gacagcctgt tttttgttta
     6781 tggttatttc ttatccgtat ttttaaattt gaacgcattg cgttcaaccg tgtgcaacac
     6841 acacggctgg cgaacacggt tcgcccctac attacaataa ttcataaaat ctacgaattg
     6901 gtaactgttc cgtccgtcac ggtgtcgctt gtgtccctga ataaaagtgt aatgcac
//
LOCUS       DAOMVE010000105         6955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2905_length_6955_cov_3.947826, whole genome shotgun sequence.
ACCESSION   DAOMVE010000105 DAOMVE010000000
VERSION     DAOMVE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2905_length_6955_cov_3.947826"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /gene="rsmG"
                     /locus_tag="PLS64_06670"
     CDS             complement(<1..518)
                     /gene="rsmG"
                     /locus_tag="PLS64_06670"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRL43538.1"
                     /translation="MAIKDTLTTVTDGFKTKLDDEMLQRFEIYFKLLVEWNEKMNLTA
                     ITDEHGVAVKHFADSLTLYNFVDVKKNASIIDVGTGAGFPGVVLKIARPDIKLTLLDS
                     LNKRLTFLDTVLNNTGLDAELVHSRAEEGGKNKLYREKFDIAVSRAVARLNVLCEYCL
                     PYVKVGGLFVAM"
     gene            complement(518..2392)
                     /gene="mnmG"
                     /locus_tag="PLS64_06675"
     CDS             complement(518..2392)
                     /gene="mnmG"
                     /locus_tag="PLS64_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HRL43539.1"
                     /translation="MNYFAGEYDVAVVGAGHAGIEAALAAARLGCKTAIFTINMDAVG
                     NCPCNPSIGGTAKGHLVREIDALGGEMGKTADECFLQSRMLNRGKGPAVHSLRAQIDR
                     RKYSSVMKHKIELQKNLDLRQAEITDIEKTEGGYNLTTRMLAVFKCKTVIIATGTYLG
                     GRIFVGEVSYESGPDGIFPAAFLGSSLKKLGLPLRRFKTGTPARVLKSSIDFSELEVQ
                     KGDEPPQPFSYETENLGENKVECHVSWTNDETKQIILENINRSPLYAGKIEGVGPRYC
                     PSFEDKIMRFKDKPRHQLFIEPCGLDTEEMYLQGMSSSLPEEVQLKFYHTIKGLEHCL
                     IMRPAYAIEYDCVDPLAMNPTLEFKDFEGLFGAGQFNGSSGYEEAAAQGLVAGINAAM
                     KVLGREPVIFDRSESYIGTLVDDLVTKGANEPYRMMTSRSEYRLVLRQDNADERLTPL
                     GHRIGLISDERYAKFLKKQELKKEELNRLKSTVISPTDEVNEILVSRETSEITSGVRL
                     IDLMKRPQLGYDALKNIDKTRPELDPNIFEQVEIEIKYEGYIQKQLKQVEQMKKLEVK
                     QLPKDFDYNEIEGLRLEAREKLNKIKPLNIGQASRISGVSPADISVLLIWLAQNNRRQ
                     "
     gene            complement(2401..3768)
                     /gene="mnmE"
                     /locus_tag="PLS64_06680"
     CDS             complement(2401..3768)
                     /gene="mnmE"
                     /locus_tag="PLS64_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRL43540.1"
                     /translation="MNYDTVAAISTAQGEGGIGVIRISGDDALRIADKIFNSVSGKKV
                     TEMKGYTASFGKISEDGEDIDEAVALVFKAPHSYTGEDVVELSCHGGLYITKRVLRAV
                     LNAGAVPAQAGEFTKRAFLNGKIDLTEAEAVIDIISAKSRGAARSALCVKEGALRKKI
                     DGVKNDLLSMAAHLSAWADYPEEDIAEVTADSVIETCDNAIKCFKSLLDSYDCGQAVK
                     KGIDTVIAGRPNVGKSTLMNLLSGYEKSIVTDIPGTTRDIVEDTVTVGDVVLNLSDTA
                     GLRDTEDTVEKIGVDRARKRLEQCGLLLAVFDNSRELDEDDYALIESAKLVPSIAVIN
                     KTDLDNKLDIEYISKNIKYIVYISAISGDGRDELTKAVEKIAGTQNFNPSEGVLSNER
                     QRIAVKNALDSVVEAMNALKCGMTFDAVTVSIEDAISHLLELTGERTSDEVVDRVFHN
                     FCVGK"
     gene            complement(3811..4620)
                     /gene="jag"
                     /locus_tag="PLS64_06685"
     CDS             complement(3811..4620)
                     /gene="jag"
                     /locus_tag="PLS64_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRL43541.1"
                     /translation="MKSGDIVIKEAIGIGDNEVDALNDAKKQLGLDENAEVDFEVIQR
                     AEKKKFGLFGGQPAKVRVTLKETPAEKAEQFLRDVLNNMKLESVVIEKKEIEGGIEFN
                     LSGEDVGFVIGRRGETLDSLQYLTSLVANHSDNSYFKVTIDTGNYREKREKTLEILGR
                     KLAFKAVKTGRKTNLEPMNPYERRIIHTSVQKVNGAISWSEGENANRHVVIGPDPKAK
                     PVRRNGGYNNRGRGGRRPYSANRSEHNTPANPDRKPLNEGGATGLYGRIDK"
     gene            complement(4625..5632)
                     /locus_tag="PLS64_06690"
     CDS             complement(4625..5632)
                     /locus_tag="PLS64_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRL43542.1"
                     /translation="MQIFGFLGSLLGYVLWGAFYLLKDFGLSIIVFTIIVRLCMFPFT
                     IKQQKSMAGTARLSKKQKEIQEKYKNNRQKAQEEIYKLYEKEGVKPTGGCLTMIVPFL
                     VLFGVFYAVAYPLTNTLHIDSAKVTEALNYVNTIPGYTAASGATNATYQEIYFLKDFS
                     CFQNIDAIQQIFSADQLNTITMFEKGFNTFGMNLFAIPQDYGLWSPMILFPVICFASN
                     VLTQFITMRINGKNNPMQQQQGCMKVMMYAMPLFSAYIAYIVPSAVAFYWIVSSLVSL
                     VQSVIVGKLFSPQRMTATSEARHAALMFEQEALVQYNYVPHGLSESAEENTNSKKKKK
                     K"
     gene            complement(5642..5881)
                     /gene="yidD"
                     /locus_tag="PLS64_06695"
     CDS             complement(5642..5881)
                     /gene="yidD"
                     /locus_tag="PLS64_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRL43543.1"
                     /translation="MKRILLAIIRFYRTSVSPLTASHCKYIPTCSQYGIEAIERFGAL
                     KGTALTVWRILRCNPFSKGGYDPVPEKKPKSKKLK"
     gene            complement(5886..6227)
                     /gene="rnpA"
                     /locus_tag="PLS64_06700"
     CDS             complement(5886..6227)
                     /gene="rnpA"
                     /locus_tag="PLS64_06700"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRL43544.1"
                     /translation="MSEYITLKDNRDFSGLYRRGKSFVGPVLVTYVLKNKSDNLRFGI
                     TTGKKIGKAVRRTRSRRVIREAYYKLYPDIKKGYDFVFVARGKTPYVKSTVVYKEMKK
                     QLSLAGVLKEG"
     gene            complement(6294..6428)
                     /gene="rpmH"
                     /locus_tag="PLS64_06705"
     CDS             complement(6294..6428)
                     /gene="rpmH"
                     /locus_tag="PLS64_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRL43545.1"
                     /translation="MLRTYQPKKLHRKKEHGFRKRMSTSNGKKVLARRRAKGRKRLSY
                     "
BASE COUNT         1933 a         1589 c         1268 g         2165 t
ORIGIN      
        1 ttcatcgcaa caaacaaacc gcctaccttg acatacggca agcagtattc gcagagaaca
       61 ttcaaccttg caactgctct tgaaacggca atatcaaact tttcacgata aagcttattt
      121 ttaccgccct cctctgccct gctgtgaaca agctctgcat caagtcctgt attgttaaga
      181 actgtgtcga gaaatgtaag tcttttgtta aggctgtcaa gaagcgtcag cttaatgtca
      241 ggtcttgcaa ttttaagcac aacacccgga aaaccggcac ctgttcccac atcaataatg
      301 cttgcattct tctttacatc aacaaagttg taaagtgtaa ggctgtcggc aaaatgcttg
      361 accgcaacac cgtgttcatc ggttattgca gtaagattca tcttttcgtt ccactcaaca
      421 agcaatttga agtagatttc aaacctttgg agcatttcat cgtcaagttt ggttttaaaa
      481 ccatcagtaa cggtagtaag agtatcttta attgccatta ctgcctcctg ttgttctgag
      541 caagccagat aagaagtacg ctgatatccg caggagaaac gcccgaaatt cttgacgcct
      601 gtccgatatt gagcggttta atcttattaa gcttttccct tgcctcaagt cgaagtcctt
      661 caatttcatt gtaatcaaag tcctttggca attgcttaac ttcaagcttt ttcatttgtt
      721 caacctgttt aagctgtttt tgaatataac cctcatactt aatttcaatt tcaacctgtt
      781 caaagatatt cggatcaagc tccggtcttg ttttgtcaat gttcttgagt gcatcataac
      841 caagctgagg gcgtttcata aggtcaataa gtcgtacacc cgaagtaatt tcagatgttt
      901 cacgtgaaac aagtatctca tttacttcat cagtaggtga aatgaccgta cttttaagtc
      961 tattaagttc ttctttttta agctcctgtt ttttaagaaa ctttgcgtat ctttcgtcgc
     1021 ttataagacc aattctgtga ccgagaggag taagtctttc gtcggcatta tcctgacgaa
     1081 gtacaagtct gtactcactt cttgaagtca tcattctata cggctcattc gcaccctttg
     1141 taacaaggtc atcaacaagt gtgcctatat agctttccga acggtcgaat ataacaggct
     1201 ctctgccgag aaccttcatt gcggcattaa ttcctgcaac aagtccctgc gccgcagcct
     1261 cctcataacc cgaagagccg ttgaattgac cggcaccgaa aagtccttca aaatctttaa
     1321 attcaagcgt cgggttcatt gcaagcggat caacacaatc atattcaatt gcataagcag
     1381 gtcgcataat aaggcaatgt tcaagacctt ttattgtgtg gtagaattta agctgaactt
     1441 cttcaggaag tgacgagctc attccctgaa gatacatttc ctcggtatca agtccgcacg
     1501 gctcaataaa aagctgatgt cttggcttat ccttaaaacg cataatctta tcttcaaagc
     1561 ttggacaata acggggacca acaccctcaa ttttgccggc ataaagcgga gaacggttga
     1621 tattttcaag aataatctgc tttgtttcgt catttgtcca actgacatgg cattcaacct
     1681 tgttttcacc gagattctca gtttcataag agaacggctg gggaggttca tcaccttttt
     1741 gaacttcaag ctcagaaaaa tcaattgaac ttttcagaac tcttgccgga gtacctgtct
     1801 taaatcttct taaaggcaga ccgagttttt taaggcttga accgagaaat gcggcgggaa
     1861 aaattccgtc gggaccgctt tcatatgaaa cctcaccgac aaaaattctt ccgccgagat
     1921 atgtacccgt tgcaataata acggtcttgc acttaaatac ggcaagcatt cttgtagtaa
     1981 gattataacc gccctccgtc ttttcaatat ccgtaatttc agcctgacga aggtcgagat
     2041 ttttctgtaa ttcaatctta tgtttcataa cggaagaata ttttcttctg tcaatctgtg
     2101 ctctcagcga atgaacagcc ggaccttttc ccctgttaag cattctggac tgcaaaaaac
     2161 attcatcggc agtctttccc atttcaccgc cgagtgcgtc aatctcacgg acgagatgtc
     2221 cttttgctgt tcctccgatt gacggattgc agggacagtt tccgaccgca tccatattta
     2281 tggtaaatat tgcagtttta cagccaagcc ttgccgccgc aagagccgcc tcgattccgg
     2341 cgtgacctgc accgactact gcaacatcat attcacccgc aaaataattc ataatatcac
     2401 ctattttcct acgcagaagt tgtgaaacac cctatccaca acctcatcac ttgttctctc
     2461 acccgttaat tcaagtaagt gtgaaattgc atcctcaatt gaaactgtta ctgcgtcaaa
     2521 tgtcataccg catttaagtg cgttcattgc ctcaacaacc gaatcaagtg catttttaac
     2581 ggcaattcgc tgtctttcat ttgagagaac gccttcactc ggattaaagt tttgagttcc
     2641 tgcaattttt tcaacagcct ttgttaattc atctctgccg tcaccgctga ttgcggatat
     2701 atatactata tattttatat ttttactgat atattctata tcaagcttat tatcaagatc
     2761 agtcttatta attacagcaa ttgaaggcac aagctttgca gactcaatga gtgcataatc
     2821 gtcctcgtca agttctctgc tgttatcaaa aacagcaagc aaaagaccgc attgctcaag
     2881 tctttttctc gctctgtcaa caccgatttt ctcaaccgta tcctcagtat cacgaagtcc
     2941 tgccgtatcg ctgagattca gaacaacatc gcccacagtt accgtatctt caacaatatc
     3001 tctcgtagtt ccgggaatat cggttacaat actcttttca tatcccgaaa gcaaattcat
     3061 aagcgtgctt tttcctacat tcggtctgcc ggcaattacg gtatcaatgc ctttttttac
     3121 cgcctgaccg cagtcatagc tgtcaagtaa ggacttaaaa cacttgattg cattgtcaca
     3181 ggtttcaatt acggaatcag ccgtaacctc ggcaatatcc tcctcggggt aatctgccca
     3241 cgcagaaagg tgagccgcca ttgagaggag gtcatttttt acaccgtcaa ttttcttacg
     3301 caaagcaccc tcttttacac aaagagcaga ccttgccgcc cctctgcttt tagcgcttat
     3361 aatatcaatt acagcctctg cttcggtgag gtcaattttg ccgtttagga atgctcgctt
     3421 tgtaaattct cctgcctgag ccggaacggc gccggcattg agaacagcac gcagaactct
     3481 ttttgtaatg taaagtccgc cgtggcacga aagctcaaca acatcttcac cggtatagct
     3541 gtgaggcgcc ttaaaaacaa gtgcaaccgc ctcgtcgata tcttcaccgt cttctgaaat
     3601 ttttccgaac gatgccgtat agcctttcat ctcagtaacc tttttacccg aaacggagtt
     3661 gaaaattttg tcggcaatcc gaagtgcatc gtcacccgaa attctaataa cgccgatacc
     3721 gccctcgccc tgagcggtac ttattgcggc aactgtatcg taattcataa aatcaccaag
     3781 aaaaaagggc ggttaaaaac cgccctgtaa ttatttgtct attcttccgt aaagacctgt
     3841 tgcaccgccc tcgttaaggg gctttctgtc aggatttgcg ggagtgttat gctcggaacg
     3901 gtttgcgctg tatgggcgtc tgccgcctct gcctctgttg ttataaccgc cgtttctgcg
     3961 aacgggcttt gcctttggat caggaccgat aacaacatgg cggtttgcgt tttcgccttc
     4021 gctccatgaa atagcaccgt ttaccttctg aacagaagtg tgaataattc ttctctcata
     4081 aggattcatt ggctcaagat ttgtctttct gcctgtttta acagctttga aagcaagctt
     4141 tctaccgaga atttcaagag tcttttctct tttttctctg tagtttccgg tatcaatagt
     4201 taccttaaaa tagctgttgt cagagtggtt ggcaacaagg cttgtaaggt actgaagtga
     4261 gtcaagtgtt tcacctcttc tgccgattac aaaaccgaca tcctcacccg aaagattgaa
     4321 ctcgataccg ccttcaattt ctttcttctc tatcacaacg ctttcaagct tcatgttgtt
     4381 gagaacatct ctcaaaaact gttcagcttt ttctgcagga gtttctttga gggtaaccct
     4441 aacctttgca ggctgacctc cgaaaagacc gaattttttc ttttcggctc tctggataac
     4501 ctcaaaatca acctctgcat tttcgtcaag accaagctgc ttttttgcat cgtttaatgc
     4561 gtcaacttcg ttatcgccaa tgccgatagc ttctttaatc acaatatcac ctgatttcaa
     4621 acttctactt ctttttcttc tttttggaat ttgtattttc ttctgcactc tctgaaagac
     4681 cgtgcggaac atagttatac tgaacaagag cctcctgctc aaacataaga gcggcgtgtc
     4741 ttgcttcgga tgttgctgtc attctctgag ggctgaaaag cttgcccacg ataaccgact
     4801 gaacaaggct tacgagtgaa gatacaatcc agtaaaatgc aacggctgac ggaacaatgt
     4861 aggcgatata tgccgagaaa agcggcattg catacatcat aaccttcata cagccctgct
     4921 gttgctgcat aggattgttc tttccgttga ttctcattgt gataaactgt gtaagtacgt
     4981 ttgatgcaaa acatattaca gggaaaagaa tcatcggact ccaaagaccg tagtcctgcg
     5041 gaattgcgaa aaggttcata ccgaatgtat taaatccctt ttcaaacata gtgattgtat
     5101 tgagctggtc ggcagaaaaa atctgctgaa tagcgtcaat attttggaaa cagctgaaat
     5161 ccttcaaaaa ataaatttcc tgatatgtgg cattggttgc gcccgacgcc gcagtatatc
     5221 cgggaattgt atttacatag ttaagagctt cggttacctt tgcgctgtcg atatgaagtg
     5281 tgtttgtaag aggatatgca acagcgtaga atactccgaa aagaacaagg aacggaacaa
     5341 tcatcgtaag acagccgcct gtcggcttta cgccctcttt ttcatagagc ttatatattt
     5401 cttcctgtgc cttttgtctg ttatttttat atttttcctg aatttccttt tgttttttgg
     5461 aaagtcttgc ggtacctgcc attgattttt gctgtttgat tgtaaaagga aacatacaaa
     5521 gtcttacaat aattgtaaag acgataattg acagtccgaa atctttaaga agatagaaag
     5581 ctccccacag cacatagccg agcaagcttc caagaaaacc aaagatctgc atacggttac
     5641 ctcactttaa tttcttactc tttggctttt tttccggaac gggatcatac ccgccctttg
     5701 aaaacggatt gcagcgcaga atacgccaaa cggtaagcgc tgtgcccttg agtgcaccaa
     5761 acctttcaat tgcctcaata ccgtattgcg aacaagtcgg aatgtattta caatgacttg
     5821 ctgtcaacgg agaaactgag gtccggtaaa acctgataat tgccagaaga attcgtttca
     5881 taaaactatc cctctttaag aacacctgca agcgaaagct gttttttcat ttccttgtaa
     5941 acaacagtgc ttttaacata gggtgtttta cctctggcaa caaaaacaaa gtcatatcct
     6001 ttttttatat cgggataaag tttgtaataa gcctctctta ttaccctcct tgaccttgtg
     6061 cgccttacgg cttttccgat ttttttgccg gtggttattc caaaacgcag gttatcggat
     6121 ttattcttta acacataagt taccaaaaca ggaccaacaa aggattttcc ccgtctgtaa
     6181 agtccggaaa aatccctgtt atccttcaaa gtgatatatt cactcataaa tttaccacct
     6241 tgcaaacagg tgcattaaaa aagaaagacc accggttaaa agtggtcttc tctttaataa
     6301 gacaaccttt ttctaccttt agctcttctt ctggctaaaa ctttcttgcc gttagatgta
     6361 gacattctct ttctgaagcc gtgctccttt tttctgtgga gcttcttagg ctgataagtt
     6421 ctcaacataa tgaaaaccct cccttcaaaa tataaccttg caatatagca ttatatctta
     6481 atttcagccc gtagtcaaca aaaattttta tttttttatt ttctaaaatg aaatttaaaa
     6541 ttttcttttg attttgaaaa acagcagcaa attatttttc tttatcggta aatacaatat
     6601 attgcaacac agaatttaac acacactata tatccgaatt attgtaaaat ttgcaccctg
     6661 tttgcgcact ttaataataa taaagtataa attacaataa attacaaatg gctttttgta
     6721 attgcaattt taaatttgtt gcgttataat aaagcagaat ggtattttat caaaatggga
     6781 taattatgga aaatgggtgc agtgtgttgc accgtggaag ggaaagatta aattaatgga
     6841 ctcctttgaa gacgcatgga aagttataag cgattactgt aaatctaaaa ttaccgacat
     6901 cgcctacaat acatggatca gcagaattca gcccgttgac cttgactttg acaac
//
LOCUS       DAOMVE010000106         6867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2973_length_6867_cov_2.473576, whole genome shotgun sequence.
ACCESSION   DAOMVE010000106 DAOMVE010000000
VERSION     DAOMVE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2973_length_6867_cov_2.473576"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1300
                     /locus_tag="PLS64_06710"
     CDS             38..1300
                     /locus_tag="PLS64_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43546.1"
                     /translation="MKKFLCLILALSSIFVFTLFGCTNDKSKPISQNALIDYSNDMQI
                     TYLCSGGGSSIPATKSDTGYYYIGDDRIIIYIDSKTQKATPLCAKPDCMHNDPDTCDA
                     YVNSSENISQDSLWGSLGTAIQYYQGYLYMVCGEYDKSMIEYNTYLMKMDKDGTNRQK
                     VTDYFDSPFTNWFIHQGYFYYTSDSSVLRIPLTNPKSSPEEVYKAQHFIKDNENTYSS
                     FCAYKNYIYFQVDELDEEGNGIGRQTICLNLDTMKKSFLKIGKNNASFSAFAGDSMIM
                     YYSDGNEKVYVKADLEGNNCKNIFSQKSNEIQSITGDGTYYYFDNSFQVANGSSKEQK
                     ITVCDENLKEIDTFKLPNMDTKTYNFFAPQDENCFLFECFNEKDERCLVMADKSQIGT
                     IGGNTIKFKELCKLKWADDKNNNDAVAR"
     gene            1306..>1877
                     /locus_tag="PLS64_06715"
     CDS             1306..>1877
                     /locus_tag="PLS64_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43547.1"
                     /translation="MRIFFCELYKLLSKKIFIICLVVALCINAFTLIYSSAENYNDRT
                     KHNNIEYYNSLIKNCNSSKKASEYLKKELDIINERLTENSTDKDALVEKMTLLNDLIS
                     QQNYINRYNDFINNMQIRADNQLSFSIFAEPDSFAYNNIQQTPLDFQHLKGVELYAGN
                     NTFAENATQFQITDYLMIVLVALTCILLFYT"
     assembly_gap    1878..1895
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1896..>2851
                     /locus_tag="PLS64_06720"
     CDS             <1896..>2851
                     /locus_tag="PLS64_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43548.1"
                     /translation="GLYPLVRSTKNGRTSTIVAKLFAVIAVTVVTTILFYLSNILITG
                     IYFGFGDMSRYIQSIGIFMNCSLKISIFEYLILWILGKTLTLCAFSLLFSFCFTAVKT
                     SAKIYGIIVVFLAVEITANLFIEGASAFSFFKYVNIVYFLSNNNLFGEYLNVNIFSNP
                     VNIIMVWTVVVTTLALVGFFGSVTAFTKTSQISKKSSIISVIAEFLQKHYRIRGSVKI
                     YSGEAYKHYKISFAMVILIILVLFAVSNLNDDLSIRFNDASESAYCDYMEDIEGVIDS
                     DTENYLAKEQQYFDDLETERNEIFSDKNLSETEKRNKSSYIDA"
     assembly_gap    2852..2861
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2862..3599
                     /locus_tag="PLS64_06725"
     CDS             <2862..3599
                     /locus_tag="PLS64_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43549.1"
                     /translation="KGEAFNRILEQKYYVQIKAEEIDEEPAFVNELVCKRLTEDTYRE
                     WLYFTILMAVIIFCSSNIFACEYKNSMVNLIRSNRYGKGKLIMIKLLTVMFTSLLSFT
                     LVYLPYFINFISTFGTKIFDIPIAFMSDYNLLTSSVTVGEYIAILCIVHLVVTLTATT
                     VIFMLSMLLKNNIMTMIVTSGIILIPCLVAMELTDVRMVTAFQNNIWQTVVTVIVMVC
                     LILTVISIVSVFVKFSNIKWRKKICQH"
     gene            3587..4465
                     /locus_tag="PLS64_06730"
     CDS             3587..4465
                     /locus_tag="PLS64_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43550.1"
                     /translation="MSTLEIRNISKTYKKGAVKALDNFNVVLTPGVYGLLGPNGAGKS
                     TLMNIITDNLNADSGDVLYDGENIKKLGKNYRSVLGYMPQQQGLYDDFTLNRFLWYMA
                     ALKGLKKKEAKEKITSLLDTVNLTNAAHKKLGGFSGGMKQRALIAQALLNDPKILILD
                     EPTAGLDPKERIRIRNFISEIAEDKIVLISTHVVSDIEFIAKEIILLKQGKLVSHDTC
                     QNLVNEIANKVVEMEIEKEELKYFQDNYRVSNLYHNDDKILVRLVTDNPPENHKITIA
                     NPTLEDLYLYIFEQGM"
     gene            4648..5088
                     /locus_tag="PLS64_06735"
     CDS             4648..5088
                     /locus_tag="PLS64_06735"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRL43551.1"
                     /translation="MEQYMPIVWIGFAIFMLLCEGFTTQLVSIWFVLGSVCAAITTIF
                     TSDITIQSAVFLVVSLVSLIVTRPLVKRFKKRNKTTGTNADRHIGQVGFMLTDLTDID
                     KTAQAKVGAETWTVKSDKAPLLKGTKVKVLAIEGVKLIVEPVTE"
     gene            5099..6004
                     /locus_tag="PLS64_06740"
     CDS             5099..6004
                     /locus_tag="PLS64_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRL43552.1"
                     /translation="MLGLIILGIILLILLIVIVSNVKVVPQAHAYVIERLGTYHVTWS
                     TGLHVKIPFIDKISKKVSLKEQVIDFPPQPVITRDNVTMQIDTVVYFEITDPKLYTYG
                     VERPLSAIENLTATTLRNIIGDLELDNTLTSRDTINGKIRVILDEATDAWGIKVIRVE
                     LKNILPPREIQDAMEKQMKAERERRARILDAEGEKRSQILVAEGMKESAILKADAVKE
                     QKIREAQGEAEAILTVQKANADALKMLNEASPTDRIIQLKSLEAFGKAADGKATKIII
                     PSDIQGLAGNVTAIKELWKDDVPKA"
     gene            6083..>6867
                     /locus_tag="PLS64_06745"
     CDS             6083..>6867
                     /locus_tag="PLS64_06745"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HRL43553.1"
                     /translation="MQFLLDLLWYFVIFSFFGWVASSFRSLLLEKKFSNNGFLTSPFC
                     PMYGFSAVICYTALKPFENSKLILFIGSTLILSALMVVVGVLVEKTLKFKPWDFSSSK
                     FSIGNYITFPYALFLGLLGMLLVGLIIPVLRTAVEAIPFWVSLILVLCFCGIIVIDYV
                     FSMITTIRLLRRIAKLKNSSELMDKGATEVEIQELEENYNRLFTENILRKRLAHAYPD
                     LTHMAYVKQINAKIEEIKQDNMKEYTQTFESKEDKPFAYGFCFT"
BASE COUNT         2274 a         1120 c         1328 g         2117 t
ORIGIN      
        1 tgctacaagc actaagtaaa acaaaggtgg tattctgatg aaaaaatttt tatgtttaat
       61 tttagcgttg tcaagtatat ttgtttttac tttatttgga tgcacaaacg ataaaagcaa
      121 gccaatttca caaaacgcac taattgatta ttcaaacgat atgcagataa catatttgtg
      181 cagcggtggt ggctcttcga ttcctgcaac aaaatcagac actggatatt attatattgg
      241 tgatgacaga ataattattt atatcgatag caaaacacag aaagcaactc cgctttgcgc
      301 aaaacctgat tgtatgcaca atgatcctga tacttgtgat gcatatgtaa attcatcaga
      361 aaacatatcg caggattcac tatggggatc acttggaacg gcaattcagt attatcaggg
      421 ttatttgtat atggtttgcg gtgaatatga taaaagtatg attgaatata atacctattt
      481 gatgaaaatg gataaggacg gaacaaacag acaaaaagtc accgattatt ttgatagtcc
      541 attcacaaat tggtttattc atcaaggcta cttttattac acctcggact cttctgttct
      601 cagaataccg ctcacgaacc caaaatcaag cccagaagag gtatacaaag ctcagcattt
      661 tatcaaggat aacgaaaata catattcttc attctgtgca tataagaatt atatttattt
      721 tcaggttgac gagctcgacg aagaaggaaa cggtattggc agacagacta tttgcctaaa
      781 tcttgataca atgaaaaaat cttttcttaa gatcggcaag aacaacgcat ctttttctgc
      841 gtttgcgggg gacagtatga ttatgtatta ttccgatggt aatgaaaaag tgtatgtaaa
      901 ggcagattta gagggcaata attgcaaaaa tatattttca caaaaaagca acgaaattca
      961 atcaataaca ggcgatggaa catattacta ttttgataat agttttcaag tcgcaaatgg
     1021 ttcatcaaaa gaacagaaga taactgtttg tgatgaaaac ttaaaagaaa tcgacacatt
     1081 taaattgcca aatatggata ccaaaacata caactttttt gcccctcagg acgaaaattg
     1141 tttcctattt gaatgtttta acgagaaaga tgaacgttgc cttgttatgg cggataaaag
     1201 tcagattgga acaattggcg gaaatactat aaaattcaaa gaactgtgca aactcaagtg
     1261 ggctgatgat aaaaataaca acgatgctgt tgcaaggtaa ttattatgag aatatttttt
     1321 tgtgaactat ataagttact gtcaaaaaag atttttataa tatgtcttgt tgtcgcctta
     1381 tgtataaatg cttttacatt gatatattca tctgccgaaa attataatga cagaactaag
     1441 cataataaca tagagtatta taattcttta attaaaaact gtaactcttc gaaaaaagca
     1501 agtgaatatt taaaaaaaga gttggatatt atcaatgaaa ggttaacgga gaatagtacc
     1561 gataaggatg cacttgttga aaaaatgact cttttgaacg accttatctc acagcaaaat
     1621 tacataaaca gatataatga tttcataaat aatatgcaaa taagggcgga taatcaattg
     1681 tccttttcca tatttgcaga acccgatagt tttgcttata ataatataca acaaacaccc
     1741 ctggattttc agcatctaaa gggtgttgag ttatatgcag gcaacaatac atttgcagaa
     1801 aatgcaacgc agtttcaaat aacagattat ctgatgattg tacttgttgc cttaacttgt
     1861 atattgcttt tttacacnnn nnnnnnnnnn nnnnnggatt gtatccccta gttcgtagta
     1921 caaaaaacgg aagaacatca acaatagttg caaaactgtt tgcagtgata gctgttactg
     1981 tcgttacaac tatccttttc tacttatcga atatactgat aacaggaata tactttggct
     2041 ttggagatat gagcaggtac attcagtcaa tagggatctt tatgaattgt tccctaaaga
     2101 ttagtatttt tgaatattta attctttgga tattagggaa gaccctaact ttgtgtgcat
     2161 tttcattgtt gttttccttt tgtttcacag ctgtgaaaac ttcagcgaaa atttacggaa
     2221 ttattgttgt atttttagcc gttgaaatta ctgcaaacct ttttattgag ggtgcatcgg
     2281 cattttcatt ctttaaatat gtaaatattg tttatttcct gtcaaacaac aacttgttcg
     2341 gtgagtactt gaatgtaaat atattttcaa atcctgtcaa tataatcatg gtatggacgg
     2401 tggttgtaac aaccttagct ttggtcggct tttttggcag tgtgacagca tttacaaaaa
     2461 catctcaaat atcgaaaaaa tcttcaataa tttccgtcat tgcagaattt cttcaaaaac
     2521 attatagaat tcgaggaagt gtaaaaatat acagcggaga ggcatacaag cattataaaa
     2581 tctcctttgc aatggttatt cttataattc ttgtgttgtt tgcagtatct aatttgaatg
     2641 atgatttatc aattcgtttc aacgatgcaa gcgaaagtgc ttattgtgac tatatggagg
     2701 atattgaggg tgtaattgat tccgatacag agaattatct tgcaaaggaa caacagtatt
     2761 ttgatgacct tgaaactgag cggaatgaga tttttagtga taaaaacctg agtgaaacgg
     2821 agaaaagaaa taaatcaagt tatatagacg cnnnnnnnnn naagggtgaa gcatttaata
     2881 gaatattgga acaaaaatac tatgttcaaa ttaaagcaga ggaaatcgac gaagaacctg
     2941 cttttgttaa tgaattggtg tgcaagcgat taacagagga tacatacagg gaatggcttt
     3001 attttactat tcttatggcg gtcataatct tttgttcatc taacatattt gcttgtgaat
     3061 acaaaaattc aatggtcaat ttaatacgca gtaatcgtta tggcaaagga aaattgataa
     3121 tgataaaact acttactgtt atgtttacct cactcttatc ctttacattg gtgtacttac
     3181 catacttcat caattttatt tctacttttg ggactaaaat ttttgatata cccattgcgt
     3241 ttatgtctga ttataatctg ctaacttcct cggtaacagt gggagaatat atagcgattt
     3301 tgtgtatagt ccatcttgtt gtgacattaa ctgctactac tgttatattt atgttgtcaa
     3361 tgttgcttaa aaacaatatt atgacgatga tagttaccag cggaattatt ctgatacctt
     3421 gtcttgttgc tatggaactt actgacgtga gaatggtaac agcttttcaa aataatattt
     3481 ggcaaactgt ggtgacagtt atagtaatgg tatgtttgat tttgactgtg atatcaatag
     3541 tatcagtctt tgttaaattc agcaacataa agtggaggaa gaagatatgt caacattaga
     3601 aattagaaat ataagcaaaa cctacaaaaa aggagcagta aaggctcttg ataattttaa
     3661 tgttgttctt actcccggag tgtacggtct cttaggaccg aacggtgcag gaaagtcaac
     3721 gcttatgaat attatcactg acaatcttaa tgcagacagc ggagatgtgc tttatgacgg
     3781 cgagaatata aaaaagcttg gtaagaacta tcgttctgtc cttggctata tgccgcagca
     3841 acagggatta tatgatgatt ttactctcaa tcgctttctg tggtatatgg cggcattaaa
     3901 gggattaaaa aagaaggagg ctaaggaaaa aataacctca cttcttgata cggtaaatct
     3961 cactaatgcg gcacataaaa aactcggcgg attttcggga ggtatgaaac aaagagcctt
     4021 gattgctcag gcacttttga atgacccgaa aattttgatt cttgatgaac cgactgcggg
     4081 ccttgacccc aaagagagaa ttcgtatccg caattttata agtgaaattg cagaagataa
     4141 aatcgtgctc atttctactc atgttgtgtc cgatatcgag ttcattgcta aagagattat
     4201 acttctaaag cagggaaaac ttgtgtcaca cgatacctgc caaaatcttg taaatgaaat
     4261 tgcaaataag gtagttgaga tggaaattga aaaagaagaa ttaaaatatt ttcaggataa
     4321 ttatcgagtt tcaaatctct atcataatga tgataaaatc ttagtaagat tagtcacaga
     4381 taatccccct gaaaatcata aaataacaat tgcaaaccca acccttgaag atttgtacct
     4441 ctatattttt gaacaaggaa tgtgatatta aaattattac cgacaagtaa tggtgttcta
     4501 tgctaatact tgtcggtatt tttatgccct ttttacatga aattgtaagg ataaagcgga
     4561 atacagatac aaaataatca gttaacgcat aaaatagcaa tatataatat tgtgttaaaa
     4621 ttatgcgaaa aggagggtga cagctctatg gagcaatata tgccgatagt gtggataggc
     4681 tttgcaatat ttatgttgct ttgcgaaggc tttacaacac agcttgtatc aatatggttc
     4741 gttctcggct cggtttgtgc cgcaataaca actatattca caagcgacat aacaatccag
     4801 tcggcggtgt tccttgttgt gtcacttgta tcgctgattg tgacacgacc gcttgtaaaa
     4861 cgctttaaaa agcgtaacaa aacaacggga acaaacgcag accgccacat cggtcaagtc
     4921 gggtttatgc tcacagattt aaccgatatt gacaaaacag cacaggcaaa ggtcggtgcg
     4981 gaaacttgga cggtaaagtc cgacaaagca ccgcttttaa aaggaacaaa agttaaggtt
     5041 cttgcaatcg agggcgtaaa gctcatagta gaacccgtaa cagaatgaga ggtaaataat
     5101 gttaggactt attattttag gaattatcct tttaatcctg cttatagtaa tcgtaagcaa
     5161 tgtcaaggta gtaccgcagg cacacgcata tgtaattgaa agactcggaa catatcatgt
     5221 aacatggtca acaggcttgc atgtaaaaat tccgtttatc gacaaaattt caaagaaagt
     5281 ttcactaaaa gagcaagtga tcgacttccc tccacaaccc gtgatcacaa gagataacgt
     5341 tacgatgcag attgacacgg ttgtgtattt tgaaattacc gatccaaagc tttatactta
     5401 cggtgtagaa agaccgctca gcgctattga aaatcttact gcaacaacat tgcgtaatat
     5461 catcggtgat cttgagcttg ataacacact cacatcccgt gatacaatca acggtaaaat
     5521 cagagtgatt cttgacgagg caaccgacgc atggggcatt aaggtaatcc gtgtcgagct
     5581 taaaaacatt cttccgcccc gtgaaattca ggatgcgatg gaaaagcaga tgaaggcaga
     5641 gcgtgagcgc cgtgcaagaa ttcttgatgc agagggtgaa aaacgcagtc agattctcgt
     5701 tgcagagggt atgaaggaat ctgcaattct taaagccgac gctgttaagg agcagaaaat
     5761 ccgtgaggca cagggtgaag ctgaggctat tctcacagtt cagaaagcta atgccgacgc
     5821 acttaaaatg ctcaatgagg cttctcctac cgatagaatt attcagctta aatcgcttga
     5881 ggcattcggt aaagccgctg acggcaaggc tacaaagatt atcattcctt cagatattca
     5941 gggacttgcg ggtaatgtta ccgcaattaa agaactctgg aaagatgatg tgccaaaggc
     6001 ataaaaatgt gaaatttacg gcaatttttc gggcacagaa ttctgtgtcc gatttgttgc
     6061 ataaaattgt gggggatagg aaatgcaatt tttactcgac ctgttgtggt acttcgtaat
     6121 tttctcgttt tttggctggg tggcgagcag tttccgttcg cttcttcttg agaaaaaatt
     6181 cagtaacaac ggttttttga catcaccgtt ttgccctatg tatggatttt cggcggttat
     6241 ttgttatacg gcactaaaac cgtttgaaaa cagcaagctg attttattca tcggttcaac
     6301 tctgatatta tctgcactta tggttgtggt cggtgtgctt gtagaaaaaa ctctcaaatt
     6361 caagccatgg gatttttctt cctcgaaatt cagcataggc aattatataa cttttcctta
     6421 tgcacttttt ctcggattgc tcggtatgct gcttgtcgga ttgataattc cggtgcttcg
     6481 cacagccgtt gaagcaattc ctttctgggt aagtcttatt cttgttcttt gcttctgcgg
     6541 aattattgta attgattatg ttttttcaat gataacaaca atccgtctgc tcagaagaat
     6601 tgcaaagctt aaaaattctt ccgagcttat ggataaaggt gcaacagagg ttgaaattca
     6661 ggaacttgaa gaaaactata accgactttt tacagagaat attttaagaa aaagacttgc
     6721 acacgcttat cccgatctca ctcatatggc ttatgtaaag caaattaatg ctaaaattga
     6781 ggagattaag caggataata tgaaagaata tacacaaacc tttgaaagca aagaggacaa
     6841 gccgtttgct tacggattct gttttac
//
LOCUS       DAOMVE010000107         6865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_2974_length_6865_cov_3.348899, whole genome shotgun sequence.
ACCESSION   DAOMVE010000107 DAOMVE010000000
VERSION     DAOMVE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2974_length_6865_cov_3.348899"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..2439)
                     /gene="pheT"
                     /locus_tag="PLS64_06750"
     CDS             complement(70..2439)
                     /gene="pheT"
                     /locus_tag="PLS64_06750"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRL43554.1"
                     /translation="MFLSMNWIQDFVDLSGLDKIELIRKFSLSTAEVENDILRKGSEI
                     SGIVVGEIKSVENHPDSKKLHLLKIDAGEDELIDVVCGAPNVRVGLKTAFAKVGAKIG
                     EITITPRPLAGFTSNGMCCSEAEIGISDDNSGIMEITDDVKNGTDLKDIYEIDDIVFE
                     VDNKSLTNRPDLWGHYGIAREFAALAGRELKPFDLDDLKAYDGLKKIDMKIEDTLCQR
                     YSCLQIENINRNVSPVNMRIRLFYCGMRAINYLADLTNYLMLEMGQPMHAFDSRKVEK
                     IRIKRFDKPFTFKTLDGVERNIDENTLMICNGNTPVAIAGIMGGLDSEIVDDTTTLTL
                     ESATFNAVSVRKSTVRLAHRTDASMRYEKCLDPEMTVTAIARFVYLLKKYDSDIKVVS
                     SLTDEYAFRYDTVVLDFDKNFVDRYTGIEISNDRIVATLESLGFKVELNNDNFSVTVP
                     SWRATKDVTIKADIIEEITRIYGYDNFEIHTTRSPLYPVRASVVKTTENKIKDMLVKR
                     YNLHELHSYVWAYNDELKAIDIPVEDNVKLANATNPNIETLRNSIVPTQLCQIKSNLS
                     FSNDFGIFEIGRVVNGLDENGLCIENKKLAITLYSKTRNVKDIYFELRDILAVVTDEI
                     KHKALTFEKAEPTHSYEHPVNLYSVCLDGRNIGTIGIVHPTVNKKIDKKAAIVFAEID
                     VNAYANTEAAPIVYDEPSKFPPMDYDISVEVPQGMFFSHLAECWKNEGGEILKSTKIV
                     DTYDTDTVHSVTIRFEFSSNERTLSSAEVQKIMDKIISNLSENGVKLRG"
     gene            complement(2455..3507)
                     /gene="pheS"
                     /locus_tag="PLS64_06755"
     CDS             complement(2455..3507)
                     /gene="pheS"
                     /locus_tag="PLS64_06755"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRL43555.1"
                     /translation="MNEKLLSLKDEIKSRLGETQDLTDLDKIRVSYLGKKGSITALLK
                     GMKDLPNEERKTFGAEVNKLKAQAAQLIEERLAELKEKEIQREIALMPQFDITSPVAL
                     ARGSYHPITLVQRECEKIFSSMGFNIEDYSEIVTDYECFEALNIPKHHPARDMQDTYY
                     LDNGQLLKSHTSAAQNAILKKYGKGLIENGTPIKAIFPGRCFRNEATDACHENTFFQM
                     EGVMVDKDISISNLIYFMKTMLSEVFKKDIKVRLRPGFFPFVEPGFELDISCLICGGE
                     GCPSCKHSGWLELCPCGMIHPNVLREGGIDPEKYTGFAFGLGLTRLAMMKYGIKDIRD
                     LNSGSLKALSQFTDDE"
     misc_binding    complement(3553..3789)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3904..4446
                     /locus_tag="PLS64_06760"
     CDS             3904..4446
                     /locus_tag="PLS64_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43556.1"
                     /translation="MNNFNEQVVRRVNKPKNLIIKIVSVLLLILVPTTLFLLAFATIA
                     YLIYVAFFVFVGGIYAVWYVFSIQKVDFEYSVSGNELEIAKVVALRKRKTMCKVQINE
                     IERLEKDDTEIKKMRFLKTFIAARDIDSKDENYFAVFNSPAYGRCLLVFTPNEDILNG
                     MKPHLNKNIVVQLFYKRKMS"
     gene            4451..5323
                     /locus_tag="PLS64_06765"
     CDS             4451..5323
                     /locus_tag="PLS64_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRL43557.1"
                     /translation="MTVTDVNVVKSAIFERPDDANKGTLGSLLCVCGSYGMAGAAVMS
                     AKGALRTGIGLAKCALPRSIYPIVAQTILESVYYPLSENADGRISKVNVPFLVSESCK
                     SSATLVGCGLGVCDDTLKIVFSLIENSTTPLVLDADALNCVAKQPEILLRKKAPIIIT
                     PHPGEMSRLTGLATPLVNAERERVALDFAQKYGVVTVLKGAGTVIASPNGKVLVNPTG
                     NSGMATGGSGDVLAGMTASILAQGASAFSSAAAAVYLHGLAGDIAAEKYGKISMLPTD
                     LINCIPLAYKRCGF"
     gene            5400..5807
                     /locus_tag="PLS64_06770"
     CDS             5400..5807
                     /locus_tag="PLS64_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004853444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43558.1"
                     /translation="MMKKILCLVMCVFLVIGLSACGGDISKVKTHDVDSEIYSADDIN
                     SAVDTIEKEFDANWNGCTLTEIYYAGDDYCTDFHEFADRNNADEVIVLLSSFDVDSSG
                     GDGSLNPNSTYNNWNWILVRTKGGKWQHIDHGY"
     gene            5885..6406
                     /locus_tag="PLS64_06775"
     CDS             5885..6406
                     /locus_tag="PLS64_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43559.1"
                     /translation="MARKILIALCVLFVMPVFVITGCDEVQENLTPKSSFIADFSCEY
                     RKMNITGKLSVSGKKLINISLDSPNTVSGLSVTYKGSDLEISRENMICSADEAYIPES
                     SFPNITKEILYGIADGRAVFEGKCEDVCTYRLDSAFGKAVVSTNSKGCISKISVDGED
                     YEMVLSDVKDANG"
     gene            6510..6857
                     /locus_tag="PLS64_06780"
     CDS             6510..6857
                     /locus_tag="PLS64_06780"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRL43560.1"
                     /translation="MNIRRGDIYYADLSPVVGSEQGGIRPVLIVQNNVGNRFSPTVIA
                     AAITSQQSKANLPTHIPLHADSSGLAKDSVVLLEQVRTIDKKRLKEKMGTVDENSMNE
                     INNAISISFGLNA"
BASE COUNT         1929 a         1379 c         1490 g         2067 t
ORIGIN      
        1 ttttaatttt atcttatgct tgtcagatta tgtcacaaaa aggggacgga aaaaccgccc
       61 ccataatttt tagcctctga gctttactcc gttttcagaa agatttgaaa ttatcttgtc
      121 cataatcttc tgaacctcgg ctgatgaaag tgttctttca tttgatgaga actcaaagcg
      181 gattgttacg ctgtgaactg tatctgtgtc atatgtgtca acaatctttg tgcttttgag
      241 aatctctccg ccttcgtttt tccaacactc tgcaaggtgt gagaagaaca ttccctgagg
      301 aacttcaacg ctgatgtcgt agtccatcgg cgggaacttt gacggttcgt cataaacaat
      361 cggagcggcc tctgtgtttg cataagcgtt cacatcaatt tctgcaaata caattgcggc
      421 ttttttgtca atcttcttgt ttactgtcgg atgaacaatg ccgattgtac cgatatttct
      481 gccgtcaagg cataccgaat agaggtttac ggggtgttcg tagctgtgag tcggttcagc
      541 cttttcaaat gtgagagcct tgtgcttgat ttcatccgtt acaactgcga gaatgtcacg
      601 gagttcaaaa tagatgtcct taacatttct tgtcttgctg taaagtgtga ttgcaagctt
      661 tttgttctca atgcaaagac cgttttcatc aagaccgttt acaactctgc cgatttcaaa
      721 aataccgaaa tcgtttgaga atgaaaggtt tgatttaatc tgacaaagct gtgtgggaac
      781 aattgaattt cttaatgttt caatattcgg gttagttgcg tttgcaagct taacattatc
      841 ctcaacagga atgtcaattg ccttgagttc atcgttgtat gcccatacat atgagtgaag
      901 ttcgtggaga ttgtacctct taacaagcat atccttgatt ttgttttccg tagttttaac
      961 aacgcttgct cttacggggt agaggggaga gcgtgttgtg tgaatttcaa agttgtcata
     1021 gccgtaaata cgggtgattt cttcaatgat gtcagccttg attgtaacat cctttgttgc
     1081 tctccagctt ggaaccgtta cgctgaagtt atcgttgttg agctcaacct taaagccgag
     1141 gctttcgagt gttgcaacaa ttctgtcgtt gctgatttca atgcctgtgt agcggtcaac
     1201 aaagtttttg tcaaagtcaa gaacaactgt gtcatacctg aatgcgtact cgtctgtgag
     1261 cgaagaaaca accttgatgt cgctgtcata ctttttgaga agatatacaa atcttgcaat
     1321 tgcagtaaca gtcatttcag gatcaagaca tttttcatat ctcattgatg cgtctgttct
     1381 gtgagcaagt cttactgttg actttcttac ggaaactgcg ttgaatgttg cagattcaag
     1441 tgtgagggta gttgtatcgt caacaatttc cgaatcaaga ccgcccatga tgcctgcaat
     1501 tgctacagga gtgttgccgt tgcaaatcat aagtgtgttt tcgtcaatgt ttctttcaac
     1561 accgtcaagt gttttgaaag tgaagggctt gtcaaaacgc ttgattctga ttttttcaac
     1621 ctttcttgaa tcgaaagcgt gcatcggctg tcccatttca agcataaggt agtttgtaag
     1681 gtcggcaaga tagtttattg ctctcatacc gcagtagaaa agacggattc tcatatttac
     1741 ggggcttaca ttgcggttga tgttctcaat ctgtaagcag ctgtatctct gacagagagt
     1801 gtcctcaatc ttcatatcaa tctttttaag accgtcatat gcctttaaat cgtcaaggtc
     1861 aaaaggttta agctctctgc ctgcaagtgc ggcaaattct cttgcgattc cgtagtgtcc
     1921 ccaaaggtcg ggacggtttg taagcgactt gttatcaacc tcaaaaacga tatcgtcaat
     1981 ttcataaatg tccttaaggt ctgtgccgtt tttaacatca tctgtaattt ccatgatgcc
     2041 ggagttgtca tcgctgatac cgatttcagc ctcactgcag cacataccgt ttgatgtaaa
     2101 gccggcaaga ggtcttggtg taatcgtgat ttcgccgatt ttagcgccaa cctttgcaaa
     2161 agcggtttta agaccgactc ttacattcgg agcaccgcag acaacatcaa taagttcatc
     2221 ttcgcctgca tcaattttga ggagatgaag tttctttgaa tcgggatgat tttcaaccga
     2281 cttaatttca cctacaacaa taccgctgat ttcactgccc ttgcggagaa tatcgttttc
     2341 aacctcggct gtggaaagag aaaatttgcg gataagttca attttatcaa gaccgctcaa
     2401 atcaacaaag tcctgaatcc agttcataga taaaaacatt ttgtatctcc tctcttattc
     2461 gtcatcagta aactgtgaaa gtgctttaag actgccgctg ttaaggtcac ggatatcctt
     2521 gattccgtac ttcatcattg caagtcttgt aagaccgaga ccaaacgcaa agcctgtgta
     2581 tttttcggga tcaattccgc cctcacgcag aacattggga tgaatcatac cgcaggggca
     2641 tagctcaagc caaccgctgt gcttacatga cggacaaccc tcgccgccgc agataagaca
     2701 gctgatgtcg agttcaaaac ccggttcaac gaatgggaaa aagccggggc gaagtctaac
     2761 cttgatatcc tttttgaata cctcggaaag cattgttttc ataaagtaga taaggtttga
     2821 aattgaaatg tccttgtcaa ccataacgcc ttccatctgg aaaaatgtgt tttcgtgaca
     2881 agcgtccgta gcctcgtttc tgaagcatct gccggggaag atggctttta taggtgtgcc
     2941 gttttcaata agtcccttgc cgtacttttt aagaattgcg ttctgtgcgg ctgatgtgtg
     3001 tgactttaaa agctgaccgt tgtcaaggta gtaagtgtcc tgcatatctc ttgcggggtg
     3061 gtgcttagga atgttaagag cctcaaagca ttcgtagtcg gttacgattt cggagtagtc
     3121 ctcaatgtta aatcccattg atgagaaaat cttttcgcat tctctctgaa caagggtgat
     3181 tgggtgataa gaaccccttg caagtgcaac aggcgatgta atgtcaaact gcggcatcaa
     3241 ggcgatttca cgctgaattt ccttttcttt aagttccgca agtctttctt caataagctg
     3301 tgcggcctgt gctttgagct tgtttacctc agcaccgaaa gtttttcttt cctcgttggg
     3361 aaggtctttc atacccttta aaagagccgt gatgctgccc tttttgccaa gataagaaac
     3421 tctgatttta tcaaggtcgg tcaaatcttg tgtttcgccg agtcttgact tgatttcgtc
     3481 ttttaaactt aataatttct cgttcataaa ttacctccgt aaaaataata aagccctgcg
     3541 taaaatcaat tacgcaggga cgaaataatc cgtggtacca ccctgatttt tgcatcaaag
     3601 caaacactcc cgaaagcaga cactttcaca acctgtaacg gagtcagccg ttgagatcta
     3661 ctgaaaattt caacctcacc actcggaagg gaacttcaca cctgcaacac cgttgtatgc
     3721 tttcagcctc ggcacacatt ctctttgacg gtatcacgca aacgctactt tcttcgtcat
     3781 catgttatct ttttctatga taacaccaaa acattttttt tgcaagagtt tttgtatctt
     3841 ttgttgaaaa aaacaacagc ctgtgatatc atatgaaata tcaatcataa aggagaaaat
     3901 acattgaata attttaacga acaggttgta agacgggtta acaaacctaa aaatctgatt
     3961 ataaaaattg tttcggtgct tttgcttatt ttagtgccga caacattgtt tttacttgct
     4021 tttgcaacaa ttgcatatct tatctatgtg gcttttttcg tgtttgtagg cggaatttat
     4081 gctgtttggt atgtgttcag tattcagaag gtcgattttg aatattcggt ttcgggcaat
     4141 gagcttgaaa ttgcaaaggt tgttgcattg cgcaaaagaa aaaccatgtg caaggttcag
     4201 attaacgaga ttgaaagact tgaaaaagac gataccgaaa tcaagaagat gagatttctc
     4261 aaaactttta tcgccgcaag agatattgac tccaaggacg aaaactattt tgccgttttc
     4321 aacagtcctg catacggcag atgtcttctt gtttttactc cgaacgagga tattctcaac
     4381 ggtatgaaac cgcacctcaa caagaatatt gtggttcagt tattctataa aagaaaaatg
     4441 agttgattgt atgacagtaa cagatgtaaa tgttgtaaaa agtgcaattt ttgaacgccc
     4501 cgatgatgca aacaaaggaa ctctgggttc attgctctgc gtttgcggaa gctacggaat
     4561 ggcaggggcg gcggttatgt cggcaaaggg tgcgttaaga acgggcatag gtcttgcaaa
     4621 atgtgccttg ccgagaagta tttacccgat tgttgcccaa acgattcttg agagcgttta
     4681 ctatcctctc agcgaaaatg cagacggcag aatctcaaag gtaaatgttc cgtttttagt
     4741 cagcgaatcg tgcaaatcct ctgcaactct tgtcggttgc ggactcggtg tgtgcgacga
     4801 tacattaaaa attgtatttt ctttaataga aaattccaca acaccgcttg tgcttgacgc
     4861 agacgcactc aattgtgttg caaaacagcc tgaaattctt ttaaggaaaa aagcaccgat
     4921 tataattact cctcacccgg gcgaaatgtc aagacttaca ggtcttgcaa ctccgcttgt
     4981 aaatgcggaa agggagagag ttgccctcga ttttgcacaa aagtacggtg ttgtaaccgt
     5041 tctcaaagga gcgggaacgg taattgcctc tccaaacggc aaggtgcttg tgaacccgac
     5101 aggtaattcg ggaatggcaa caggcggcag cggtgatgtg cttgcaggaa tgaccgcatc
     5161 aattcttgca cagggcgcaa gtgcgttttc atcagccgca gcggcagtat atctccacgg
     5221 ccttgcaggc gacattgccg ccgaaaaata cggcaaaatc tcaatgctcc ccacagacct
     5281 gataaattgc atcccccttg cctataagcg ttgcggattt tgatttaaaa tacaagtaag
     5341 aaacgggaat ctttttgcga aaaaaagttg tcaaaatgat gacgattttg aaaggagtaa
     5401 tgatgaagaa aattttatgt ttagttatgt gtgtatttct tgttatcggt ttgagtgcct
     5461 gcggaggaga tataagcaaa gtgaagactc acgatgtgga ttctgaaatc tattccgcag
     5521 atgatattaa ctctgctgtt gatactattg aaaaagagtt cgacgccaat tggaacggct
     5581 gtaccctcac ggaaatttat tatgcaggag atgattactg caccgatttt catgaatttg
     5641 ccgacaggaa caatgcagat gaagttattg tgctgttatc ttcatttgat gttgattcat
     5701 caggcggcga cggatcatta aatccgaatt ccacatataa taactggaac tggattttag
     5761 tcagaacaaa aggcggaaaa tggcagcata ttgaccacgg atattaagat aaaatgcatt
     5821 aatcacttat ttgtacaatg tattaaaaat ttcacgggac atataatgta tcgggaggtg
     5881 tcttatggca cggaaaatct tgattgcatt atgtgtcctt tttgttatgc ctgtttttgt
     5941 cattacgggg tgtgatgaag tgcaggaaaa tctcactccg aaatcttcgt ttattgccga
     6001 tttcagctgt gaatacagaa aaatgaacat cacgggaaag ctgtcggtaa gcggcaaaaa
     6061 gctcatcaac atttcgcttg attctccgaa cacggtcagc ggtttgtcgg ttacatataa
     6121 gggttctgat cttgaaattt caagggagaa tatgatttgc tccgccgatg aagcgtacat
     6181 tcccgaaagc agtttcccga atattacaaa agaaatttta tacggaattg cagacggcag
     6241 ggcggttttt gaggggaagt gcgaagatgt gtgtacatac agacttgatt cagcgttcgg
     6301 caaagccgtt gtcagcacaa attcaaaggg ctgtataagc aaaatcagcg ttgacggcga
     6361 agattacgaa atggtgcttt ccgatgtgaa agatgcaaac ggctgaaccg attcacaaat
     6421 tttacgctca aaaatattat gtaatagctg tataaaagct tttggaaagg caaaaataca
     6481 agtaaacaat tatttggagc gtgaaagcct tgaatatacg aagaggagat atctattatg
     6541 ccgacctgag tcccgttgta ggctctgagc agggcggaat cagacctgtg cttattgttc
     6601 agaataatgt cggcaaccga ttcagtccga ctgtaatcgc cgccgcaatt acaagtcagc
     6661 agtcaaaggc gaatttgcct acccacattc cattgcacgc cgacagctcg ggactcgcca
     6721 aagacagcgt tgttttgctt gaacaggtac gaacaatcga caaaaaacgg ctcaaagaaa
     6781 aaatgggtac tgttgatgaa aattctatga acgaaataaa taacgcaata tcaatctcgt
     6841 tcggtcttaa cgcataattc cgcat
//
LOCUS       DAOMVE010000108         6818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3008_length_6818_cov_3.550643, whole genome shotgun sequence.
ACCESSION   DAOMVE010000108 DAOMVE010000000
VERSION     DAOMVE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3008_length_6818_cov_3.550643"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /gene="rplB"
                     /locus_tag="PLS64_06785"
     CDS             complement(<1..731)
                     /gene="rplB"
                     /locus_tag="PLS64_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019680102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRL43561.1"
                     /translation="MAIKVYKPTINSRRNMSVTDYSGLSKVEPEKSLLAPLNKKAGRN
                     SYGRITVRHRGGGNRKKYRIIDFKRQKFDVEGTVKTLEYDPNRSAFIALVEYTDGEKA
                     YIIAPEGLKVGDKVTAGANADIKPGNALPLTAIPVGTFIHNVELYPGRGAQLARSAGN
                     MAQLMAKENGLALLRLPSGELRNVPENCMATIGQVGNTDHENVKIGKAGRKRNMGWRP
                     TVRGSVMNPNDHPHGGGEGKSPVGRP"
     gene            complement(834..1127)
                     /gene="rplW"
                     /locus_tag="PLS64_06790"
     CDS             complement(834..1127)
                     /gene="rplW"
                     /locus_tag="PLS64_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRL43562.1"
                     /translation="MIAHDIVIRPVITEKSMLGAVNKIYTFEVAKSANKIEIAKACEE
                     VFGVKVAKVNTVSMRGKFRRQGRNNGGYSKSWKKAIVKLTEDSKPIEFFEGMM"
     gene            complement(1124..1747)
                     /gene="rplD"
                     /locus_tag="PLS64_06795"
     CDS             complement(1124..1747)
                     /gene="rplD"
                     /locus_tag="PLS64_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRL43563.1"
                     /translation="MPNLAVVDMEGKNVGTIELAESVFGIEPNAAVMHQMVVNYLAAQ
                     RQGTQSALTRSEVSGGGKKPWRQKGTGRARQGSTRAPQWTHGGVVFAPKPRDYRFSVN
                     KKVRRLAMKSAFSSKVMENELIVVDSISMDEYKTKKIVAMLSAIGAEKKALIVLPEVD
                     SKVIKSANNIPGVKTAQVNTLNVYDILNADKLIIVKDAVSKIEEVYA"
     gene            complement(1764..2396)
                     /gene="rplC"
                     /locus_tag="PLS64_06800"
     CDS             complement(1764..2396)
                     /gene="rplC"
                     /locus_tag="PLS64_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRL43564.1"
                     /translation="MQKAIIGKKIGMTQIFDEKGIMIPVTVVEAGPCVVTAKKTVEND
                     GYASVQLGYGDVKPHKVNKPMKGFFDKAGVAPKKTLKEFRFTDTDAYSIGDLVKADVF
                     TPGDKIDVTGKSKGKGTAGVIKRWNFHRLKESHGTGPCVRHGGSIGACSSPSRVWKGK
                     RMAGHLGAEQVTVQNLTVVKVDAENNLIAVKGAIPGPNKGIVVIKDSVKA"
     gene            complement(2470..2781)
                     /gene="rpsJ"
                     /locus_tag="PLS64_06805"
     CDS             complement(2470..2781)
                     /gene="rpsJ"
                     /locus_tag="PLS64_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRL43565.1"
                     /translation="MAVKEKIRIRLKGYDHQLVDQSAERIVATAKRTGARVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDILRPSNKTVEALMSLELPAGVDIEIK
                     L"
     assembly_gap    3168..3177
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3410..3925
                     /locus_tag="PLS64_06810"
     CDS             3410..3925
                     /locus_tag="PLS64_06810"
                     /inference="COORDINATES: protein motif:HMM:NF014614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRL43566.1"
                     /translation="MKHLYFGDGKGKTTAAVGIAVRAAGSNLKVLFVQFLKTEFSGER
                     HVLSHTENVTLTLCPADLKFTFQMNEKEKAQAAKIFKGIFDHSVTLALTEKYDMVVFD
                     EIIDAINAEMLTESEVVEFITNAPSSMEIIMTGHNPSQKMIDLCDYVTEFKKIKHPYD
                     RGITGRIGVEF"
     gene            complement(4154..5311)
                     /locus_tag="PLS64_06815"
     CDS             complement(4154..5311)
                     /locus_tag="PLS64_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HRL43567.1"
                     /translation="MRFADNRNKIGKYIPAVVFLIFIYGMALWFIFSPKPDYSSSEKR
                     YLQKFPEVTAEKLLSGDFGSEFETFFADRFPQRNTWVGLNAYTALAVGNNGASGVYNC
                     KNGYLINKPVSTENNLDKNIGAVADFAKSIDTPTTVMLVPSTGYIVDDVLPTFHDKYN
                     DDEDISKISSTLSKDKIGFVDLRERFKSEYKNGSQLYYKTDHHWTTKGAYTGYQELCK
                     ALGITPIDDSTLKKDSYPDFYGTTYSSSGFWLTPPDNIEIWNNPKNSDRNISVKITEG
                     ANIKTSGSMYFTDHLKEDDKYPVFIDGNHALTEITNTNAKNGTILLIKDSFSHSLAPF
                     LAENYSKVVLVDLRYYKESVSDLVTTYNPEQVVVLYGIDNLATDTDIVWLK"
     gene            complement(5351..6745)
                     /locus_tag="PLS64_06820"
     CDS             complement(5351..6745)
                     /locus_tag="PLS64_06820"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRL43568.1"
                     /translation="MVFSSLVFMFAYLPITLLAYYLVPRQGRNIFLFIVNLIFYGWGE
                     PKLVLLMVFNIFFNYIGGWLVDKYRADAKKKKLFLILTCVLDIGILAVFKYTGMITET
                     LNMLPFLNIPELQISLPIGISFYTFQTMSYVIDVYRDDAPVSKNFINFGTYVALFPQL
                     IAGPIVRYRDVAEQLVNRRETLEMFTRGVKLFMIGLAKKVIIANTMGTLTTNIFATTD
                     ENGVVGTWVGMIAYTFQIYFDFSGYSDMACGLGNMMGFEFLKNFNYPYIAKSITDFWR
                     RWHISLSTWFKEYVYIPLGGNRKGVKRQILNLLIVWGLTGLWHGAAYNFVLWGLYYGL
                     LLILEKFVLKKFLDRLPSFVQHIYTLFIIIIGWGLFYFTDVGQLGEFMVDLFNFGNGI
                     CGDQAFNLIMSNLPMLIIAAVASTPLATMLYTRFEHRRFMWIPETLYCMGVLVVSTAS
                     LVNQSYNPFLYFRF"
BASE COUNT         1831 a         1526 c         1416 g         2035 t
ORIGIN      
        1 ggacgaccaa caggtgactt accctcacca ccgccgtgcg gatggtcgtt agggttcatt
       61 acagaaccac gaacagtagg acgccaaccc atgtttctct tacgacctgc tttaccgatc
      121 ttaacatttt cgtgatcggt gttacctacc tgaccgattg tagccatgca gttttcagga
      181 acgtttctga gctcgcctga aggaagtctt aaaagtgcaa gaccgttttc cttagccatg
      241 agctgagcca tgttgccggc tgaacgagcg agctgagcgc ctctgccggg ataaagctct
      301 acattgtgga taaatgtacc aacagggatt gctgtaagcg gaagtgcgtt accgggctta
      361 atatcagcgt ttgcacctgc tgttaccttg tcgcctacct taagtccttc cggagcaata
      421 atgtaagcct tctcgccgtc tgtgtactct acaagagcaa tgaatgcaga acggtttgga
      481 tcgtactcta atgttttaac tgtaccttca acatcaaatt tctgacgctt gaagtcaata
      541 atacggtact ttttgcggtt gccgccgcct ctgtgacgaa cggtgattct gccgtagctg
      601 ttacgacctg cctttttatt taaaggagca agcaagcttt tttccggctc aacctttgaa
      661 agacccgaat aatctgtaac cgacatattt cttcttgagt taatggtcgg cttatatact
      721 ttaatggcca tttggtttca ctctcctcat gatggctttg cgggaatatg gcatttccca
      781 tacattctgc ctgaataatg tttttcagac tcaatactga acctgagtca gtattacatc
      841 atgccttcaa aaaattcgat aggcttgctg tcttcggtaa gttttacgat tgctttcttc
      901 cagctctttg agtagccgcc gttgtttctg ccctgacgac ggaatttgcc tctcatgctt
      961 actgtgttta cctttgcaac cttaacgccg aaaacttctt cacaagcttt tgcaatttca
     1021 atcttgtttg ctgacttagc aacttcaaag gtataaattt tgttaactgc gccgagcata
     1081 ctcttttcgg taataacagg gcggattaca atatcatgag ctatcatgca tatacctcct
     1141 caatcttgct taccgcatcc ttaacgatga taagcttgtc agcattcaaa atgtcatata
     1201 cattaagagt gtttacctga gctgttttaa caccggggat gttgttagct gatttaataa
     1261 ccttgctgtc aacctcagga agaacaatga gagccttctt ttcagctccg attgcgctga
     1321 gcattgcaac aatcttcttt gtcttgtact cgtccatgct gattgaatca acaacgatga
     1381 gctcgttctc cataaccttt gaagagaaag ctgacttcat agcaagtctt cttaccttct
     1441 tgtttacgga aaatctgtaa tctcttggct tcggagcgaa tacaacgccg ccgtgtgtcc
     1501 actgcggagc tcttgttgaa ccctgtcttg cacggcctgt acccttctgt ctccacggct
     1561 ttttaccgcc gcctgaaacc tctgatcttg taagagcgga ctgtgtgccc tgacgctgtg
     1621 ctgcgaggta gttaactacc atctgatgca taacagctgc atttggttcg ataccgaata
     1681 cggactcggc aagctcgatt gtaccgacat tcttgccttc catatctact actgctaaat
     1741 ttggcattta taatccccct tccttaagcc tttacggaat ctttgattac aacgattccc
     1801 ttgttaggac ccggaatagc acccttaaca gcgatgaggt tgttttctgc gtcaacctta
     1861 acaactgtga ggttctgaac cgttacctgt tctgcgccga ggtgacctgc cattctctta
     1921 cccttccaaa ctcttgaagg tgatgagcag gcaccgattg aaccgccgtg gcgtacgcaa
     1981 ggacctgtac cgtgtgattc cttaagacgg tggaagttcc atctcttaat aacacctgct
     2041 gtacccttac ctttgctctt gcctgttacg tcaatcttgt cgccgggggt gaatacgtct
     2101 gctttaacga gatcaccgat gctgtaagcg tctgtgtctg taaagcggaa ctctttgaga
     2161 gttttctttg gtgctacacc ggccttatca aagaagccct tcattggctt gtttaccttg
     2221 tgaggtttaa catcgccgta gccgagctgt accgaagcgt agccgtcgtt ttcaactgtt
     2281 ttcttggctg ttaccacgca agggcctgcc tcaacaacag ttacgggaat catgattccc
     2341 ttttcatcga aaatctgtgt cataccgatt ttcttaccga tgattgcttt ctgcataaat
     2401 tgtatcctcc ttataggtta ttggttggtt tcccaacatg accctgtctg cttgtaggtt
     2461 ggtatccgat tataacttaa tctcgatatc aacgccggca gggagctcta agctcataag
     2521 agcctcgact gtcttgtttg acggtcttaa gatgtcgata agacgcttgt gagttctgat
     2581 ttcaaactgc tcacggctgt ccttgtactt atgaacagca cggagaattg ttacaacctg
     2641 cttctcagtc gggagcggaa cgggacctga aactcttgcg cctgtgcgct tagctgttgc
     2701 cacgattctc tctgcagact gatcaacgag ctgatgatca taacccttta atcttattct
     2761 aatcttttcc ttgactgcca ttatcgtcgc ctccttaaaa atgttagttg gcgggcgttg
     2821 cttttcaaaa ctttttgaaa ctctgccggg tgtttcctca ccccgcatta aaaaacccgt
     2881 ttaactattc agttaaccgg gaagttctga aagcgtttga gcgcagttat ggcaaagcaa
     2941 ccccattgcc actcaatcgc tctgtgtttc aatttaatcg cccggtttaa aatcggacat
     3001 actccacgga aaaaccggct taatgccgca acctcccgct tcatcgcata tcttgtgcag
     3061 tcacacaagc attattataa acgaaatacc gcattatttc aaccttttcg gcaaaataag
     3121 cggtatagaa gtttttaaaa tatttttaat taattttgtt taatttgnnn nnnnnnnctg
     3181 actttgtaaa cacaaaggct gtcggctcgt ttttctttac tgcttttaaa aaacttaatg
     3241 gaaatatttt ctttaaaata tttttaatta attttgttta atttgtacat actcccgttt
     3301 gtcgatttta tgttataatt aggcagacga aaatgtcttt tctatactat tgtatataag
     3361 ggattccatt ttgtcaattg tgcagtttta tgaaaaggaa tgacagacaa tgaaacattt
     3421 gtattttggt gacggtaagg gcaaaaccac agccgctgta ggaattgccg tcagggcggc
     3481 aggcagtaac ctcaaggtgc tgtttgtgca atttttaaaa acggaatttt cgggtgaacg
     3541 ccatgttctc agtcacaccg agaatgtgac tctcacgctc tgccccgccg acctcaagtt
     3601 cacatttcag atgaacgaaa aggaaaaggc tcaggcggca aaaattttca agggtatttt
     3661 tgaccacagt gtcacacttg ccctgaccga aaagtacgac atggttgtat ttgacgagat
     3721 tattgacgca atcaatgctg aaatgctcac cgaatccgag gttgttgagt tcatcaccaa
     3781 cgctccctca agcatggaaa tcattatgac aggtcataac ccgtcacaaa aaatgattga
     3841 cctttgcgat tatgtgaccg agttcaagaa aatcaaacat ccctacgatc gaggcatcac
     3901 cggcagaatc ggtgttgaat tttaatttta agtttgtgta gatagtttaa tttacaaaaa
     3961 cataaaacgc tcgagcgtag cgcttataaa atcgtatccg acaccacccg atttatgggt
     4021 ggttattttt ttgcattgtt ttatggggaa tgtgggatat aactgattaa aaaattacaa
     4081 aaaacggctg tcacttgacg aaagtcaatt catcagcaac agccgttttt aattcaattg
     4141 aatattaaag aacttacttg agccatacga tatccgtatc ggttgcaagg ttgtcaattc
     4201 cgtaaagcac aacaacctgt tcggggttgt aggttgtcac aaggtctgac acgctttcct
     4261 tataataacg caggtcaacc agaaccacct tgctgtaatt ttctgcaagg aacggtgcaa
     4321 ggctgtgcga aaagctgtcc ttgatgagaa gaattgtgcc gttctttgca ttggtgtttg
     4381 taatctccgt gagagcgtga ttgccgtcaa taaacacggg gtacttgtcg tcctctttga
     4441 gatggtcggt aaagtacatt gaacccgaag ttttaatatt tgcaccctcg gtaattttta
     4501 cggagatatt cctgtcggaa tttttagggt tattccaaat ttcgatatta tcgggcggag
     4561 tgagccaaaa accgcttgaa gaatatgttg tgccgtaaaa gtcggggtag ctgtcttttt
     4621 tcagggttga atcatcaatc ggagtaatgc cgagtgcctt gcaaagctct tggtagcccg
     4681 tgtaagcacc ctttgttgtc caatggtggt cggttttgta gtaaagctga ctgccgtttt
     4741 tgtattcgct cttaaagcgt tcacgcaaat cgacaaatcc gattttatcc tttgaaagcg
     4801 ttgaagaaat cttgcttata tcctcatcat cgttgtattt atcgtggaat gtcggaagca
     4861 catcatcaac aatatatcct gttgacggaa cgagcataac cgttgtcgga gtgtcgattg
     4921 attttgcaaa atccgcaacc gcaccgatat tcttatcgag attgttctca gtcgaaacag
     4981 gcttgttgat aaggtagccg tttttgcagt tatacacacc gcttgcaccg ttgttaccca
     5041 ctgcaagggc ggtgtatgcg ttaagaccta cccatgtgtt tctctgcgga aacctgtcgg
     5101 caaagaatgt ttcaaactca ctgccgaaat cacccgaaag caatttttcg gctgtaacct
     5161 ccggaaactt ctgcaagtat cttttttcgg aagagctgta gtcgggtttc ggagaaaaga
     5221 taaaccacaa agccattccg tatatgaaaa tcagaaatac aaccgcagga atgtattttc
     5281 cgattttatt tctgttatca gcaaaacgca tatatattca cctgactttt tctaattgtt
     5341 cacagccgtt ttaaaatctg aaataaagga acgggttgta gctctgatta acaaggcttg
     5401 ccgtgctgac aacaagaaca cccatacagt agagtgtttc gggaatccac ataaagcgtc
     5461 tgtgttcaaa gcgtgtataa agcattgttg caagcggagt tgacgcaaca gccgcaataa
     5521 taagcattgg caggttgctc ataataagat tgaacgcctg atcaccgcaa attccgttgc
     5581 cgaaattgaa aaggtcaacc ataaactcac cgagctgtcc gacatccgtg aagtagaaaa
     5641 gcccccaacc gatgattatg ataaacagag tgtaaatgtg ctgaacaaat gacggcagtc
     5701 tgtcgagaaa ttttttgagt acgaattttt caagaataag aagaagtccg tagtaaagtc
     5761 cccaaagtac aaagttgtat gccgcaccgt gccaaagtcc tgtaagtccc cacacgataa
     5821 gaaggttgag aatctgcctt ttaacgccct ttctgttacc gccgagagga atataaacat
     5881 attctttgaa ccatgtcgaa agggaaatat gccatcttct ccaaaagtct gtaatcgact
     5941 ttgcgatgta gggatagtta aagtttttaa gaaactcgaa tcccatcata ttgccaagac
     6001 cgcaagccat atcggagtaa cccgagaaat caaagtaaat ctggaatgta taggctatca
     6061 tacccaccca tgttccgaca acgccgtttt catcggttgt tgcaaaaatg tttgttgtga
     6121 gcgttcccat ggtgtttgcg ataattacct tttttgcaag accgatcata aagagcttca
     6181 cgccccttgt gaacatttca agcgtttcac ggcggtttac aagctgttcg gcaacatcac
     6241 ggtaacggac aatcggacct gcaataagct gcggaaagag tgcaacataa gtgccgaagt
     6301 tgataaagtt tttcgacact ggcgcatcgt cacggtaaac atcaataaca tagctcattg
     6361 tctggaatgt gtagaagctg atgccgatcg gcaggctgat ttgaagctcc ggaatattga
     6421 gaaacggcag catattgagc gtttcggtta tcattcccgt atatttaaac accgcaagaa
     6481 tacctatatc aagcacacag gtaagaatca gaaacagctt tttctttttg gcgtcggctc
     6541 tgtatttgtc aacaagccaa ccgcctatat agttgaagaa gatgttgaac accataagca
     6601 gaacaagctt aggttcgccc caaccgtaga atataaggtt tacgataaag agaaaaatat
     6661 tgcgtccctg ccttggcaca agataatagg caagcaaggt aatcggcaga tacgcaaaca
     6721 taaatacaag gctcgaaaat accactcgtt ttatcctcct gcaaatttaa attcataaat
     6781 tttttcaaat accgtcctac atattttaac ttattgct
//
LOCUS       DAOMVE010000109         6599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3125_length_6599_cov_2.966840, whole genome shotgun sequence.
ACCESSION   DAOMVE010000109 DAOMVE010000000
VERSION     DAOMVE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3125_length_6599_cov_2.966840"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /gene="ftsY"
                     /locus_tag="PLS64_06825"
     CDS             <1..616
                     /gene="ftsY"
                     /locus_tag="PLS64_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRL43569.1"
                     /translation="SIILVIGVNGVGKTTTIGKLANALSKEGKKVILAAADTFRAAAI
                     EQLEIWADRSKCEIIKQKEGSDPAAVIYDAISAAKARHADVIICDTAGRLHNKKHLMD
                     ELAKINRVIDRELPDASKEKLLVLDATTGQNAVNQAEQFRQATGITGIVLTKLDGTAK
                     GGVVLAIKDGLGIPVKYIGVGEQIDDLQPFNPDAFADALFSIKE"
     gene            622..1218
                     /gene="rdgB"
                     /locus_tag="PLS64_06830"
     CDS             622..1218
                     /gene="rdgB"
                     /locus_tag="PLS64_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HRL43570.1"
                     /translation="MKFIIASNNKKKLVELERILNPLGINAVTPKEEGITLDDVEENG
                     TTFAENAFLKANAAYLKTGLPAVADDSGLCVDALDGRPGVYTARYGGEGLTDEERYIK
                     LLDEMKNVDDDKRSAHFTSSICCILPDGSKITAEGICEGKIGYEPIGKDGFGYDPIFM
                     FGDKSFAELSAEEKDAVSHRGKALRELKAKLEYYFKNN"
     gene            1237..1539
                     /gene="yhbY"
                     /locus_tag="PLS64_06835"
     CDS             1237..1539
                     /gene="yhbY"
                     /locus_tag="PLS64_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HRL43571.1"
                     /translation="MLNSKQRAFLRGLANRIDTILIVGKGEITDNIIKQADDALTARE
                     IIKGKVLENSAYSSREAAEAIAEKCSADVVQVIGSKFVLYRRNDDEPVIELPKAKK"
     gene            1542..2144
                     /gene="nadD"
                     /locus_tag="PLS64_06840"
     CDS             1542..2144
                     /gene="nadD"
                     /locus_tag="PLS64_06840"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRL43572.1"
                     /translation="MKTGVFGGTFNPVHKGHIMLAEYCMDSVGLDRIIMIPTAVPPHK
                     ISKNLASENDRLNMCELACKGKKNFSVSDIEIKRQGKSYTYETVTQLKEIYPDDHLYT
                     IMGADMFLTLDRWKNPKIIFEKSSIITIPRDDENKSELEKFYKNVLKPMGADAVILPN
                     PVISVSSTFIRDNLDEFDLISGMLDKSVYDYIIKNNLYRK"
     gene            2150..2737
                     /gene="yqeK"
                     /locus_tag="PLS64_06845"
     CDS             2150..2737
                     /gene="yqeK"
                     /locus_tag="PLS64_06845"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HRL43573.1"
                     /translation="MNISDYKKIIRERMGDYRFSHSVNVAKEAKKLAKHYGADENKAE
                     IAGILHDITKEMPKEQQLQIIIDSGKILDNVQLHAPKLWHGMSGSIVVRDELGIDDED
                     ILNAIRYHTTGRAGMSLLEKVIFTADFTSEERTYSGVATMRKKSKKSLEEAMLYGYQF
                     TFKDLSKRQLAIHPDEVACYNEIVLQISQTKGKNK"
     gene            2734..3087
                     /gene="rsfS"
                     /locus_tag="PLS64_06850"
     CDS             2734..3087
                     /gene="rsfS"
                     /locus_tag="PLS64_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRL43574.1"
                     /translation="MSSYDEAILAAKALSSKKGLDIQIIKIADVSVLADYMVIATGTS
                     STHVKALADEVEYKLDQAGISVSHIEGYRSNSWILLDYIDVIVNVFDDEARDFYDLER
                     LWQDGESVDLTGIVD"
     gene            3105..5516
                     /gene="leuS"
                     /locus_tag="PLS64_06855"
     CDS             3105..5516
                     /gene="leuS"
                     /locus_tag="PLS64_06855"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRL43575.1"
                     /translation="MKYNHKAVEEKWQKIWEDKGVFHASEDTGKEKFYALIEFPYPSG
                     QGLHVGHPRPYTALDTVARKRRLEGYNVLYPIGWDAFGLPTENYAIKNHIHPEIVTKK
                     NIARFKKQIQSLGISFDWSREINTTDPEYYKWTQWIFIQLYKHGLAYKKEMNVNWCTS
                     CKCVLANEEVVNGVCERCGSEVVHKVKSQWMLKITEYADRLINDLDLVNYPDRVKAQQ
                     KNWIGRSKGAEVDFTTTTGDTLTIYTTRADTLYGATYMVISPEHPMIEKWADKISNMD
                     EIKKYQEAAARKSDFERTEVAKEKTGVRIDGVNAINPVNNKEIPIFISDYVLVSYGTG
                     AIMAVPAHDTRDWEFAKKFDLPIIEVVKGGNVQEEAYTDCAKGIMVNSGMLDGLTVDE
                     AKKKIIDWLTSEGKGHSKVNYKLRDWVFSRQRYWGEPIPMVYCEKCGYVPIDEKDLPL
                     RLPMVESYEPTDNGESPLAKMTDWINTTCPCCGGKAKRETDTMPQWAGSSWYYLRYMD
                     PHNKNAIASKEALNYWSPVDWYNGGMEHTTLHLLYSRFWHKFLYDIGVVPTPEPYAKR
                     TSHGMILGENGEKMSKSRGNVVNPDEIVDEYGADTMRLYEMFIGDFEKAAPWSKASIR
                     GCRRFVERYWNLQSVLIDGDKIRPELEGAFNKAIKKVGEDIENIKFNTAIATLMALIN
                     DISNVKSINKEELRIFSILLNPFAPHVTEEVYEACKLGNGILAEAEWPEYDESKCVDE
                     SVEIVVQVNGKIKAKLNIPVDADKDAVLDLAKNDENVKKAIDGMKIIKEIVVPKKLVN
                     LVVKP"
     gene            5647..5823
                     /gene="rpsU"
                     /locus_tag="PLS64_06860"
     CDS             5647..5823
                     /gene="rpsU"
                     /locus_tag="PLS64_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRL43576.1"
                     /translation="MAEIRLKENESLESALRRFKKQCARAGVIAEVRKREAYEKPSVK
                     RKKKSEAARKRKYR"
     gene            5896..6327
                     /gene="aroQ"
                     /locus_tag="PLS64_06865"
     CDS             5896..6327
                     /gene="aroQ"
                     /locus_tag="PLS64_06865"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HRL43577.1"
                     /translation="MKKILVILGPNLNMVGVREKGIYGEETASDIEKQIISYAESNGF
                     SAEVFQSNHEGDLIDKIHSAKGNFDGVVINAGALTHYSYALRDAIACVKIPFVEVHMS
                     NIHAREEFRHTSVIAPVCVGQIAGFGKNSYFLAINALKDLM"
     gene            6327..>6599
                     /locus_tag="PLS64_06870"
     CDS             6327..>6599
                     /locus_tag="PLS64_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HRL43578.1"
                     /translation="MQNSLFEQRLENLKTVLDSNSQAVLLTNEKNIGYFCGFFHSEGY
                     LLVTENETVLFVDFRYYEAALKKSSGCRVVCFTKLFKDLISELKNNS"
BASE COUNT         2133 a         1165 c         1490 g         1811 t
ORIGIN      
        1 gtcaattatt cttgtaatcg gagtgaacgg tgtaggaaaa acaactacaa tcggtaagct
       61 tgcaaatgca ctttcaaaag agggcaaaaa agttattctt gccgcagccg atacattcag
      121 agctgccgca attgaacagc ttgaaatctg ggcagacaga agcaagtgcg aaattattaa
      181 gcagaaagaa ggcagtgatc ctgctgctgt aatttatgat gcaatttcag ccgcaaaggc
      241 aagacatgca gatgtaataa tctgtgatac tgcgggcaga cttcataaca aaaagcacct
      301 tatggatgag cttgcaaaaa tcaaccgtgt aattgaccgt gagcttccgg atgcgtcaaa
      361 agaaaagctt cttgttcttg atgcgacaac aggtcagaat gcggttaatc aggctgagca
      421 gttcagacag gcaaccggaa ttacgggtat tgttcttaca aagcttgacg gcactgcaaa
      481 aggcggtgta gttcttgcaa ttaaggacgg tcttggaatc cctgttaaat atatcggtgt
      541 aggcgagcaa attgacgatt tacagccgtt taatcccgat gcttttgcag acgcactttt
      601 cagcattaag gaatgatgaa tatgaagttt ataattgcga gcaataacaa aaagaaactt
      661 gtcgaacttg aaagaatact taatccgctc ggaatcaatg cggttactcc gaaagaagag
      721 ggaataactc ttgatgatgt agaggagaac ggaactactt ttgccgaaaa cgcttttctc
      781 aaagctaatg cggcttattt aaaaacggga cttccggctg ttgccgatga ttcgggtttg
      841 tgtgttgacg ctcttgacgg cagacccggc gtttacacgg ctcgctatgg cggtgaaggc
      901 ttgacagatg aagaaagata cattaaactt cttgatgaga tgaaaaatgt cgatgatgac
      961 aagcgttctg ctcacttcac ttcttcaatc tgttgcattc ttcccgacgg ttcaaagata
     1021 acagccgagg gtatttgtga aggtaaaatt ggttatgagc caatcggcaa agacggattt
     1081 ggctatgatc cgatttttat gtttggcgac aagagttttg cagagctttc agcggaagaa
     1141 aaggatgcag taagtcatcg cggaaaagcg ttaagagagc ttaaagcaaa actggaatac
     1201 tatttcaaaa acaactgatt tgaaaggaat taatatatgt tgaacagcaa acagagagca
     1261 tttctcagag gacttgcaaa tcgtattgat acaattctta ttgttggtaa gggtgaaatt
     1321 acggataata tcatcaaaca ggcagacgat gctcttacgg caagagaaat tataaaaggt
     1381 aaggttcttg aaaacagtgc atattcatca agagaagctg ccgaagctat cgcagaaaaa
     1441 tgttctgcgg atgttgttca ggtaatcggt tcaaaatttg tactttacag aagaaacgat
     1501 gatgagcctg ttattgagct tccaaaggca aagaagtaat tatgaaaaca ggtgttttcg
     1561 gcggaacttt taatcctgtt cacaaaggac acataatgct tgccgagtat tgtatggaca
     1621 gcgttggact tgatcgtatt attatgatac ctacggctgt tccgccgcac aaaataagca
     1681 agaatcttgc aagcgaaaac gatcgcctta atatgtgtga acttgcgtgc aagggaaaga
     1741 aaaacttttc tgtgtccgat atcgaaatta aaagacaggg aaaaagctac acctacgaaa
     1801 ctgttacaca gctaaaagaa atttatcctg atgatcactt atatacaata atgggtgcgg
     1861 atatgtttct gactctggat aggtggaaaa atcccaaaat tatatttgaa aaatcgtcaa
     1921 taataacaat tccccgtgat gatgaaaata agtctgaact tgaaaaattc tacaagaatg
     1981 ttctgaaacc tatgggagca gatgcggtta ttctgccaaa tcccgtaata agcgtatcgt
     2041 caacattcat aagggataac cttgatgaat ttgatttgat aagcggtatg cttgataaaa
     2101 gtgtttacga ttatattata aaaaataatt tatatcggaa gtgagtgctt tgaatatctc
     2161 tgattacaaa aaaataatcc gtgaaagaat gggcgattac agatttagtc acagtgtaaa
     2221 tgttgcgaaa gaggctaaaa agctcgcaaa acattatggt gctgatgaaa ataaggctga
     2281 gattgccggt attttgcatg atataaccaa agaaatgcca aaggaacagc agttgcaaat
     2341 tattatcgac agtggtaaaa ttttagacaa tgttcaactt catgcaccaa agctttggca
     2401 cggaatgagc ggaagcattg ttgtccgtga tgagcttggc attgacgatg aagatatact
     2461 caacgcaata aggtatcaca caacaggacg agcgggaatg agtcttttgg aaaaagttat
     2521 ttttaccgct gattttactt ccgaggagcg tacatacagc ggtgtggcaa caatgagaaa
     2581 gaaatctaaa aagagccttg aggaggctat gctttacggc tatcagttta cattcaagga
     2641 tctttctaaa agacagcttg cgattcatcc cgatgaagtt gcctgctata atgaaattgt
     2701 tctgcaaata tcccaaacga aaggaaaaaa caaatgagtt cttacgatga agcaatactt
     2761 gcagcaaaag ccctgagttc aaaaaaggga cttgatattc agattataaa aattgccgat
     2821 gtttctgttc ttgccgacta tatggtaatt gcaacaggta caagctcaac ccatgtcaag
     2881 gcgcttgccg atgaggttga atacaagctt gatcaggcag gaatttcggt aagtcatata
     2941 gaaggttaca gatcaaattc atggattctt cttgactaca ttgatgttat tgtcaatgtg
     3001 tttgatgatg aggcccgtga tttttatgac cttgaaagac tttggcagga cggcgaaagc
     3061 gttgacctga caggtatcgt tgattaattg caggaggggt tactttgaaa tacaatcata
     3121 aagccgtaga agagaaatgg caaaaaatct gggaagataa gggtgtgttc cacgcaagtg
     3181 aggacacagg aaaagaaaaa ttctatgctc ttatcgagtt tccgtatccg tcgggacagg
     3241 gccttcatgt aggtcatccc cgtccgtaca cagcacttga tacagttgca agaaaacgca
     3301 gacttgaggg ttacaatgtt ctttatccta tcggttggga cgctttcggt cttcctaccg
     3361 agaactatgc aatcaagaat cacattcacc ctgaaattgt aacaaagaag aatattgccc
     3421 gttttaaaaa gcagattcag tcactcggca tttcttttga ctggagcaga gaaatcaata
     3481 caacagatcc cgaatactac aagtggactc agtggatttt tattcagctc tataagcacg
     3541 gtcttgctta taaaaaagag atgaatgtaa actggtgtac ttcgtgcaaa tgcgttcttg
     3601 caaacgaaga agttgtaaac ggcgtttgcg agcgttgcgg aagtgaagtt gttcacaagg
     3661 ttaagtcaca gtggatgctt aaaatcacag aatatgcaga cagacttatc aatgaccttg
     3721 accttgtaaa ttatcctgac agagtaaaag cacagcagaa gaactggatt ggcagaagta
     3781 agggtgcgga ggttgatttt acaactacaa caggcgacac acttacaatt tatacaactc
     3841 gtgccgatac actttacggt gctacataca tggtaatttc tcctgaacac ccaatgattg
     3901 aaaagtgggc tgacaagatt tctaatatgg acgaaatcaa gaagtatcag gaggctgccg
     3961 ctcgtaagtc tgactttgaa agaacagagg tagctaaaga aaagacaggc gtcagaattg
     4021 acggtgttaa tgctataaac cctgtaaaca ataaggaaat cccgattttt atttctgact
     4081 atgttcttgt atcatacggt acaggtgcta ttatggctgt tcctgctcac gatacccgtg
     4141 actgggaatt tgcaaaaaaa ttcgaccttc ctattattga agttgttaaa ggcggcaatg
     4201 ttcaggaaga ggcttacaca gattgtgcca agggtattat ggttaactcg ggcatgcttg
     4261 acggccttac tgttgatgaa gcaaagaaga aaattattga ctggctcaca agtgagggta
     4321 agggccattc aaaggttaac tacaaactcc gtgactgggt attctcccgt cagcgttatt
     4381 ggggcgagcc tattccgatg gtatattgtg agaagtgcgg ctatgttccg attgatgaaa
     4441 aagaccttcc gctcagactg cctatggttg agtcatatga accgactgat aacggagaat
     4501 caccgcttgc caagatgaca gactggatta acacaacctg tccttgctgc ggcggtaagg
     4561 ctaagagaga aaccgataca atgcctcagt gggcaggttc atcatggtac tatctcagat
     4621 atatggatcc gcacaataaa aatgccattg catcaaaaga ggctcttaac tactggtcac
     4681 ctgttgattg gtacaacggc ggtatggagc atacaactct ccaccttctt tacagccgtt
     4741 tctggcataa gttcctctat gatatcggtg ttgttcctac tccggaacca tacgctaagc
     4801 gtacttcaca cggtatgatt ctcggtgaaa acggcgagaa gatgagtaaa tcaagaggca
     4861 atgtcgtaaa tcccgatgaa atcgttgacg aatacggtgc agatacaatg cgtttatatg
     4921 aaatgttcat tggtgacttt gaaaaggctg ctccgtggag taaggcaagc atcagaggct
     4981 gccgcagatt cgttgagcgt tactggaatc ttcagagtgt tctcattgac ggtgataaaa
     5041 tccgacctga gcttgaaggt gcatttaaca aggcaatcaa aaaggttggc gaggatattg
     5101 aaaatataaa gttcaatact gctatcgcaa ctcttatggc tttaatcaac gatatttcaa
     5161 atgtaaagtc aattaataag gaagaactca gaattttctc aattctcctt aatccgtttg
     5221 ctcctcatgt tacggaagaa gtttacgagg cttgtaagct tggtaacggt attcttgccg
     5281 aagccgagtg gcctgaatat gacgagtcaa aatgcgttga cgaaagtgta gaaattgttg
     5341 ttcaggtaaa cggcaaaatt aaagctaagc ttaacattcc tgttgatgca gacaaagatg
     5401 ctgttcttga tcttgcaaag aatgatgaaa atgtcaaaaa ggctattgac ggaatgaaaa
     5461 ttattaaaga aattgttgtt ccgaagaaac ttgtaaacct tgttgttaag ccataattca
     5521 acttttaaaa ttaatttgac ctaaatttct acaatgctat tgacattatt cgagttataa
     5581 tgtataataa catttgcaat tatatgcaaa ttacccatgc ccggtggcag atgggaggga
     5641 agatagatgg cagagattag acttaaagag aatgaatccc ttgaaagtgc tttaagaaga
     5701 ttcaaaaagc agtgtgccag agctggcgtt attgctgagg ttcgcaaaag agaagcttat
     5761 gaaaagccct ctgtaaagcg taagaaaaaa tccgaagcag ctcgcaaacg caaatacagg
     5821 taatcaaaca ggcggacaga gcattctgtc cgctttttgt tttatcttca tatttctttg
     5881 aaaaaaggtg acactatgaa aaagattctt gttatactcg gacctaacct caatatggta
     5941 ggcgtccgtg aaaaaggaat atacggagaa gaaactgcct ccgatattga aaagcagatt
     6001 atttcatatg cagaaagcaa cggcttttct gccgaagttt ttcagtcaaa tcacgaggga
     6061 gatcttatcg ataaaattca ttcggcaaag ggtaactttg acggtgttgt aattaacgca
     6121 ggcgctctca cacattacag ctatgcttta agagatgcca ttgcttgtgt aaagattcct
     6181 tttgtagagg ttcatatgtc taatattcac gcaagagaag agttcagaca tacatctgta
     6241 atcgcacctg tttgtgtcgg tcagattgca ggttttggca aaaacagtta ctttctcgca
     6301 atcaatgcat taaaggactt gatgtaatgc aaaactcttt atttgagcaa agacttgaaa
     6361 atttaaagac ggttcttgac agtaacagtc aggctgtttt gttgactaat gagaagaata
     6421 tcggttattt ttgcggattc tttcacagcg aaggctattt acttgtgact gaaaatgaaa
     6481 ctgtcctttt tgttgatttc agatattatg aggctgcttt aaaaaaatca agcggatgca
     6541 gggttgtttg ctttactaag ttgtttaaag accttataag cgaattgaag aacaattct
//
LOCUS       DAOMVE010000110         6361 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3283_length_6361_cov_3.919283, whole genome shotgun sequence.
ACCESSION   DAOMVE010000110 DAOMVE010000000
VERSION     DAOMVE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3283_length_6361_cov_3.919283"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PLS64_06875"
     CDS             <1..759
                     /locus_tag="PLS64_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HRL43579.1"
                     /translation="RKEIDKLTEYAKDIGAKGLAYVRWVDEPNASFKKFMSEEELSAI
                     YERLGAEKGDVILIVADKNSTVLSTLGALRLVVAKRLDIIPDVFKFLWIVDFPFFEYD
                     EESGEWLAMHHPFTMPKEECLQYLDTDPSKVIAKAYDLTLNGVELSSGSMRITDYELQ
                     QKMFKALKLTDEEIEAKFGFLVEAYKYGAPPHGGMGIGLDRVTMLMCKADSLRDVTAF
                     PKVQNASELMSACPAEVDDESLEVLGIKVVNKED"
     gene            776..1561
                     /locus_tag="PLS64_06880"
     CDS             776..1561
                     /locus_tag="PLS64_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRL43580.1"
                     /translation="MSPDTIATIISVSIVVLAIAIIVTVIVSKIRHLSRTLFGTSSLI
                     EGIQGVKEDQEQMRETPRSLHGMTSVYMPMIKKDFPELDVEQYMNKTRSLLRNYFSAV
                     ESKRLGVIAEEVTPNFTNYVQGIIDQLSSVANTQHYEDVNIYDVQIARYIKNGATVTI
                     LFEASVGYFSYVTDMNNEVTFGSRENRMQTIYEVGLMYVQDADRLHNRATALGVNCPN
                     CGAPVKTLGEKFCQFCGTAIKEINIRSWKFNSVNEQTLQQRKF"
     gene            1779..3020
                     /locus_tag="PLS64_06885"
     CDS             1779..3020
                     /locus_tag="PLS64_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRL43581.1"
                     /translation="MGIIKAAINAVGGGLADSWLEVIEPSPMGDNTLICPGQLVSQNG
                     KSQNKKGSENIVSNGSVIHVYDNQMMILIDGGKIVDFTAEPGYFKVNNSSMPSLFCGQ
                     FGDSIKETFNRIKYGGIPSQAQRVFYINLQEIKGIPFGTSTPVNYFDNFYNSELMLRA
                     HGTYSIKVVEPFKFYQEVIPREAVTENKSVDFADVRAQYNEEFVGALGSAINQYSADG
                     ERISFIKSKQRLIGQYMAQTLDEEWTQARGMEVFAVGMDVSYTEDSQELINMRNKGAM
                     LSDAAVQQGYVAANISEGLKDAGSNANGAMAGFMGMGIGMNAGGNIMGGYQQNPQYRQ
                     QQPTQPQQQAQPQQTPAPQAGGAAAGSWTCSCGAVNTGKFCSECGSKKPEAPKKRFCT
                     NCGYELSANAKFCPECGTKTE"
     gene            3098..4222
                     /locus_tag="PLS64_06890"
     CDS             3098..4222
                     /locus_tag="PLS64_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HRL43582.1"
                     /translation="MATVNYTCPNCAAPLKFNPDKQMFSCEYCMSDFTEKYVQDFFAK
                     KEQKENSAEKQEKKEQEKAKENAAKQAQSNDSAQAENKTEEEAVIYNCPSCGAQVVTT
                     ASTAATTCFYCQNPVVLGGRLSGEFKPDRVIPFKLSKQKAIDKFLEMCKRKWFLPKNF
                     VSEKHFDKLTGVYFPYWYVDEQRSAQMVAKGEKVRSWTVGDDRYTETKVFELHRAGNL
                     IVNNVFERALKGQDRDMLQCVHPYDLGEAHPFAMSYLSGFQAEKRDIEQNEVAQAVQT
                     RINGYCQQLMKDTASGYSAVQIQNYNDKIDLENWNYTLLPVWVVTYKYRGKILPFAVN
                     GQTGKTYGELPTSAGKLLAFAAIIAAALMALGLLGGLLFL"
     gene            4219..5037
                     /locus_tag="PLS64_06895"
     CDS             4219..5037
                     /locus_tag="PLS64_06895"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRL43583.1"
                     /translation="MMNVTKRLSAMLAAMIMVFCTPLFVFAAGQTELRDDAELFTSEE
                     ETQLQAELDAFAQKTGWVAVIYTNNEGKTEDNIKPFANRYYADKYGKTTAGVILTIDM
                     KGRAIDFRTKGDAMYYFGDGRVNTLLDDVQRDMKQGNYYSAAEQFLYYVDHYYDEGID
                     DDESNENIDLQEKEDNKFLYVVKHYGIVIAGGSIIVAVIVVIVVKNRYKHNGKAGTYD
                     LKKNSKTMLTDKKDIFLTKHVSVTRIRSDSDSSGGSSSSSSGGGGSSGGGSRSF"
     gene            5127..6173
                     /gene="hydE"
                     /locus_tag="PLS64_06900"
     CDS             5127..6173
                     /gene="hydE"
                     /locus_tag="PLS64_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRL43584.1"
                     /translation="MRNIEIIQKLNTMHTACESELLQLITTYDEKDFEYLRKCARDTA
                     DRIFGNKIYIRGLIEFSSICKNDCFYCGLRRSNSNAVRYRLSKDEILSCCQNGHELGF
                     RTFVLQGGEDGCFTDDVMCDIVSEIKNRFPDCAVTLSLGERSYESYQRLFDSGADRYL
                     LRHETADKKHYSMLHPPEMSLENRMQCLYNLKRIGYQTGAGFMVGSPFQTPEMLVKDL
                     KFIEKLSPEMCGIGPFVPHKDTKFKNENHGDVSLVLMLLSIIRLLKPNILLPATTALG
                     TVDPLGREKGILHGANVVMPNLSPVKHRKDYSLYDNKICTGDEAAQCIGCLADRMKKI
                     GYEIVTDRGDYTEK"
BASE COUNT         1895 a         1308 c         1506 g         1652 t
ORIGIN      
        1 agaaaagaaa ttgacaagct cacagaatat gcaaaggaca tcggcgcaaa gggacttgca
       61 tatgtaagat gggttgacga gcctaacgca tcattcaaga agtttatgag cgaagaagag
      121 ctttcggcaa tttacgaaag actcggtgcg gaaaagggcg atgttatcct catcgttgcc
      181 gacaagaaca gcacagttct ttcaacactc ggcgcactcc gtcttgttgt tgcaaagcgt
      241 cttgacatta tccccgatgt attcaagttc ctctggattg ttgacttccc gttctttgaa
      301 tatgatgagg aaagcggcga atggcttgca atgcatcacc cgtttacaat gcctaaggag
      361 gaatgcctgc agtaccttga cacagatccg tcaaaggtta ttgcaaaggc ttatgacctc
      421 acactcaacg gtgttgagct ttcaagcggt tcaatgcgta tcacagacta tgaactccag
      481 cagaagatgt tcaaggcttt gaagctcacc gatgaagaaa tcgaagcaaa gttcggattc
      541 cttgttgaag cctacaaata cggcgcaccg ccgcacggtg gtatgggtat cggtcttgac
      601 cgtgttacaa tgcttatgtg caaggctgac agcctccgtg atgtaacggc attcccgaag
      661 gttcagaatg caagcgagct tatgtctgcg tgtccggcag aagttgatga cgaaagcctt
      721 gaggttctcg gaatcaaggt tgtcaacaaa gaagactgat taagggtaca attatatgag
      781 tccggataca attgcaacaa tcatctctgt gtcgatagtc gtacttgcaa tagcaattat
      841 agttacggta attgtcagta agataagaca tctttcaaga acattgttcg gtacaagctc
      901 ccttatagag ggcattcagg gcgtgaaaga ggatcaggag caaatgcgtg aaactccccg
      961 ttcgcttcac ggaatgacct cggtttatat gccaatgata aaaaaggatt ttcccgagct
     1021 tgatgttgag cagtatatga acaaaacccg ttcacttttg cgaaactact tttcggcggt
     1081 tgagtcaaaa aggctcggtg ttattgcgga ggaggtcact ccgaatttta caaactatgt
     1141 tcagggaatt atcgaccagc tttcatctgt tgcaaataca cagcactatg aggatgtcaa
     1201 tatttacgat gttcagattg cccgctatat aaaaaacggt gcaacggtaa cgatactttt
     1261 tgaggcatct gtcggttact tttcgtatgt taccgatatg aacaatgagg tgaccttcgg
     1321 cagcagagag aacagaatgc agacaatcta tgaggttggg ttgatgtatg ttcaggacgc
     1381 tgaccggctt cataacagag caaccgctct cggagtgaat tgccccaact gcggcgctcc
     1441 cgtaaaaacg ctgggagaaa aattttgtca gttctgcgga actgcaataa aagaaattaa
     1501 cattcgttcg tggaagttta attccgtaaa cgaacagaca cttcaacaga gaaaattctg
     1561 aaagtttgac ggtcgggttg tcagagccaa atgccctctt cccgaccgtc tttttgcatt
     1621 atttatgatg gcaaaacatt ggaacaataa aaaaacatat gccattcagc ctgtttttta
     1681 ataatagtat tgaaattttt cttcaaacat attattatta gtgtaacagc aatatctgaa
     1741 aaatcagcta taagctacaa aaattaagga gttaaattat gggtattatt aaagcagcga
     1801 taaatgcagt cggaggcgga ctcgcggatt catggctcga ggtcattgaa ccgtccccta
     1861 tgggcgacaa caccctcatc tgtcccggtc agcttgtttc gcagaacggc aaaagtcaga
     1921 acaaaaaggg ttctgaaaac atcgtatcaa acggttctgt aattcatgtt tacgataacc
     1981 agatgatgat tcttattgac ggcggtaaga ttgttgactt tactgcagag ccgggttatt
     2041 ttaaggtaaa taacagctca atgccgtcac ttttctgcgg tcagttcggt gattcgatca
     2101 aggaaacatt taaccgtatt aagtacggcg gaatcccctc acaggctcag agagttttct
     2161 atataaatct tcaggaaatc aagggtattc ctttcggaac atcaactccc gtaaactact
     2221 ttgacaattt ttacaattca gagcttatgc tccgtgctca cggtacatat tcaatcaagg
     2281 ttgttgagcc gttcaagttc tatcaggagg ttattccgag agaggctgtt acggaaaaca
     2341 agtctgttga tttcgcagat gtaagagcac agtataacga ggagtttgtc ggcgctctcg
     2401 gttctgcaat caaccagtat tccgctgacg gtgagagaat cagctttatc aagtcaaaac
     2461 agcgtcttat cggtcagtat atggcacaga cgcttgatga ggaatggaca caggctcgtg
     2521 gtatggaagt ctttgccgtt ggtatggatg tttcctacac agaggacagt caggagctca
     2581 tcaatatgcg caacaagggt gcaatgctct cggacgcagc agttcagcag ggctatgttg
     2641 cagccaatat ttcagagggc cttaaagatg ccggctcaaa tgcaaacggt gcaatggcag
     2701 gctttatggg tatgggcata ggtatgaatg ccggcggaaa tatcatgggc ggttatcagc
     2761 agaatccgca gtaccgtcag cagcagccga ctcagccaca gcaacaggct cagccacagc
     2821 agactcccgc tccgcaggca ggcggtgcag ccgcaggctc atggacttgc tcatgcggtg
     2881 cggtaaacac aggcaagttc tgctcggagt gcggttcaaa gaagcccgag gctcccaaaa
     2941 agcgtttctg cacaaactgc ggatatgagc tttctgcaaa tgcaaagttc tgtcccgagt
     3001 gcggtacaaa gacagaataa tctgaatttt aagtacaaac aatccctctt ctcaaacgga
     3061 gggggatgtt tcagaattta attataaggg gtaagttatg gcaactgtaa actacacctg
     3121 tccgaactgc gccgctccgc tgaaattcaa tcccgataag cagatgttca gttgtgaata
     3181 ctgtatgtcc gattttacgg aaaagtatgt tcaggatttt tttgccaaga aggagcaaaa
     3241 agagaacagc gcagaaaagc aggagaaaaa ggaacaggaa aaggctaaag agaatgccgc
     3301 caagcaggcg cagagcaatg acagcgcaca ggcagaaaac aaaactgagg aagaggctgt
     3361 tatctacaac tgcccaagct gcggtgctca ggttgttaca acagcgtcaa ctgcggcaac
     3421 aacctgcttc tattgtcaga atcctgttgt tctcggcggc agactttcgg gtgaatttaa
     3481 acccgaccgt gtgattccgt tcaagctttc aaagcagaag gcaattgaca agtttcttga
     3541 aatgtgcaaa aggaaatggt ttttgccgaa gaattttgtc agcgaaaagc attttgacaa
     3601 actcacgggt gtgtattttc cgtattggta tgttgacgaa cagcgtagcg cacagatggt
     3661 tgcaaagggc gaaaaggttc gttcgtggac tgtcggagat gaccgttaca cggaaacaaa
     3721 ggtttttgag ctccacagag caggcaatct gattgtaaat aatgtttttg aaagagcgtt
     3781 aaaaggtcag gacagagata tgctccaatg cgtacaccct tacgatttgg gcgaggctca
     3841 cccgtttgca atgtcctatc tttcgggttt tcaggcagaa aagcgtgata ttgagcaaaa
     3901 tgaggttgca caggcagttc aaacaagaat caacggctat tgtcaacagc ttatgaaaga
     3961 cacggcaagc ggatacagtg ccgttcagat tcagaactac aacgataaaa tcgaccttga
     4021 aaattggaac tataccctct tgcccgtttg ggttgtaacc tataaataca ggggtaaaat
     4081 tctgccgttt gcagttaacg gtcagacggg caaaacctac ggtgagcttc ctacctccgc
     4141 aggaaagctt cttgctttcg ctgcaattat tgccgcagcg cttatggcac tcggcttgtt
     4201 gggaggtctg ttgttcttat gatgaatgtt acaaaaagac tttccgcaat gcttgcggca
     4261 atgattatgg ttttctgtac tccgctcttt gtttttgcgg cgggacaaac agaactccgt
     4321 gatgatgccg aactgtttac ctcggaggaa gaaacacagc ttcaggcgga acttgacgct
     4381 tttgctcaaa aaaccggctg ggttgcggtt atctatacta acaacgaggg caaaaccgag
     4441 gacaatatca aaccgtttgc aaaccgttat tatgccgaca aatacggcaa aactacggcg
     4501 ggcgttatac tcacgattga tatgaagggc agagcaatcg acttcagaac aaaaggcgat
     4561 gcgatgtatt atttcggcga tggccgtgtt aatactcttc ttgatgatgt tcagcgtgat
     4621 atgaagcaag gcaactatta cagtgctgcc gaacaattcc tctattatgt tgaccactac
     4681 tatgacgagg gtattgacga tgacgagtca aacgaaaata ttgatttgca ggaaaaagag
     4741 gataacaaat tcctctatgt cgtaaagcat tacggcattg tcattgcagg cggttcaatt
     4801 attgttgccg ttattgttgt tattgtagtg aaaaatcgtt acaagcacaa cggcaaagca
     4861 ggaacttatg acctcaagaa aaacagcaag actatgctta ccgacaagaa ggatatcttc
     4921 ctgacaaagc atgtttccgt tacgagaatc cggagtgatt ccgacagctc gggcggttca
     4981 tcaagcagct cctccggtgg cggcggttca tcgggcggcg gaagcagaag cttttaatgc
     5041 tgaaaactat ttgaatttta cggagaatgc tgtattctct gtttgggggc ggcagaaatg
     5101 ccgtcctctt ttaatgtgaa aaaattatga ggaatattga aattattcaa aaactgaata
     5161 ccatgcacac agcctgtgaa agcgaacttt tacagctgat tacaacttac gacgaaaaag
     5221 attttgaata tcttcgcaaa tgtgcaagag atactgccga cagaattttt ggcaacaaaa
     5281 tctatatccg tggcttgatt gaattttcga gtatatgcaa aaatgattgc ttttactgcg
     5341 gacttcgccg ttcaaacagc aatgccgtgc gttaccgact ttcaaaggat gaaattctct
     5401 catgttgcca aaacggacac gaactcggat tccgcacatt tgtgttgcag ggcggtgagg
     5461 acggctgttt taccgatgat gtgatgtgcg acattgtgtc ggaaataaaa aatagatttc
     5521 ccgattgtgc cgtaacgctc tcgcttggcg aaaggtcgta tgaaagctat caaagacttt
     5581 ttgacagcgg cgcagacaga tatcttctcc gtcacgaaac agccgacaaa aaacactatt
     5641 ccatgttgca tccgcccgaa atgtcacttg aaaaccgaat gcaatgcctg tataatttaa
     5701 agagaatagg ttatcagaca ggcgcaggat ttatggtcgg ctcaccgttc caaactcccg
     5761 aaatgcttgt caaggattta aaatttattg aaaaattaag tcccgaaatg tgcggaatag
     5821 gtccgtttgt accgcacaaa gacacaaaat ttaaaaatga aaatcatggc gatgtaagcc
     5881 ttgtgcttat gttgctttca ataataaggc ttttaaaacc gaacattctt ttgccggcaa
     5941 caaccgcctt gggtacggtc gatccgcttg gtcgggaaaa gggcattctt cacggtgcaa
     6001 atgttgtaat gcccaacctt tcccctgtta agcacagaaa agattactcg ctctacgaca
     6061 acaaaatctg tacgggagac gaagcggcac aatgtatcgg ctgtcttgca gacagaatga
     6121 aaaaaatcgg ctacgaaatc gtaactgaca gaggagatta tacagagaaa tagtgtgaaa
     6181 aatcacacta ttataatata ttgccctcaa aatttgcctt tggcataatt ttgagatggc
     6241 tgaaatcatc atttacgcct gttcaggcaa cgcaggttaa ttaggtgatt tttaataaac
     6301 tgtttgttgc ctgaaaggct atgatgatta ttatgaataa atacgataaa aattcgctga
     6361 a
//
LOCUS       DAOMVE010000111         6312 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3316_length_6312_cov_2.399712, whole genome shotgun sequence.
ACCESSION   DAOMVE010000111 DAOMVE010000000
VERSION     DAOMVE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3316_length_6312_cov_2.399712"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLS64_06905"
     CDS             <1..365
                     /locus_tag="PLS64_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43585.1"
                     /translation="EQALAFIKYTLSDEIQEIGATASGSLSFPVNISVYDNAKLVAGS
                     RTDDNNKIIGIDNDFMKAYIDIADNVNACTLYRDVSHSYYNDNVIGDIVDNYINKGIS
                     KDKFIRQLTSATEIYLTE"
     gene            471..1238
                     /locus_tag="PLS64_06910"
     CDS             471..1238
                     /locus_tag="PLS64_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRL43586.1"
                     /translation="MPYIICFTKSLYVGDTFVGIENYIQLFQNNTFLLALKNTGIFTV
                     TAIPLLMVISFLIALFLNSFNKISSFFRSALLIPVVVPIASLICVWQLVFDDYGAING
                     LLNSLGFNTVEFFNSEWSMVMIVFIYVWKYCGFCVILFTAGIANVSQSLYESARLDGA
                     KAHQLVTKITIPMIAPTTFFVFLMELIYSFKIFREVFALFGDYPNTNVYFLQNFINNN
                     YYNLNYPRLSAASIVLSLFIIIVLFIFFCFERKHSYS"
     gene            1249..2088
                     /locus_tag="PLS64_06915"
     CDS             1249..2088
                     /locus_tag="PLS64_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRL43587.1"
                     /translation="MKDTVMKILKYILLIAVTVLFLFPVIYMLACSFMTGSQVSEMLN
                     FSDGKYKELNMIPNMATLEQFYQTLFRKPEYLLKFWNSVVCTFPTVIGQVAVSSLAAF
                     AFAKLKFPFRDKLFFIYIILLILPLQVTLVPDYMILENMNLLNNFLAVILPGTFSAFG
                     ICLLRQSIKYIPDSAIEAARVDGASYLKIFTNIILPQIKGGLITLALLCFIDNWNVVE
                     QPLIYFDNEGMYPLSVTLTDVGSSDYGIIFACGVIFMIPALLIYLYGHRDIDSPFVGT
                     DKG"
     gene            2102..2524
                     /locus_tag="PLS64_06920"
     CDS             2102..2524
                     /locus_tag="PLS64_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43588.1"
                     /translation="MKKDKLKSVVLKFSIVFFIVIALLTYFSKTINNMLLPKVKVVSV
                     QTGVIDDTAGSNDMKTHYLLPVSSVDGAGNTGIVFVINKTENGDATVEEVSVDICNSD
                     ELYCEVTSDSLFGDSQVVYKTTKSIENGSSVYIEEETV"
     gene            2521..3657
                     /locus_tag="PLS64_06925"
     CDS             2521..3657
                     /locus_tag="PLS64_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL43589.1"
                     /translation="MSKRKIVSFLLLLLLTVSVFIANIIIIENAKGQLPDVTQLTYDK
                     NSETAPKTVSELEKLFERITDKGIAFCSEGKETKVKEETVTTVLVNENWFSDYKTEIN
                     GENISSKNIDNRDKVAIIGSSLALKLFFTTDAVGKKICIDGNQYTICGVICENESLIN
                     KFSADDKQRVYVPYTTSENYGDRTVDMIVYDNSVYTGAIVEQMDLPQYYSVNLNEKNQ
                     TLSSFEHILYLAIFIGFSIIALKLWYRFSRKFFEEIKENLKLNYFLKSLKNIPLKYVA
                     LVLVFAGIPAVLLIIFNICDFSIFIPSKYIPNDNIFDISNYLNVLVDNAQTLNSQSLT
                     GNQMLVNLFSRTFTASLWLTIIFLISWNFVICYFLLDYRIKTKK"
     gene            3694..5217
                     /locus_tag="PLS64_06930"
     CDS             3694..5217
                     /locus_tag="PLS64_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43590.1"
                     /translation="MINRINKIKSKNPKLRKIFKMKKLLSALLIALLLPTMMLMSACN
                     DASKTSDDKLSFDYTTNFKYYYSNLNGINFPVTKSEKGYYVFLPNNYLYYIDRESQAA
                     TPLCNKPNCSHNSVDCNAYFNLFVNASGESANVVQYYDGNLYIVVKDEDAMGNFLGNI
                     LYKVTPDGSTREKLIEFKDGISHWLIHKGYFYFSQDKFADDIDSSSVYDSFSIKRYKI
                     NNIKSKPEEIFNSKNYSDNLRGTFQFTAYDDYIYVPVLPISEDEIKKQEESGKVTVST
                     YQEFYSININTIKINQIRNDEGDVCSPFFYNGQLTYLIDNSDTNGKFKYFTSELDGSN
                     PKLLTEMNYGDNLFANESQLYLQNFSEDTDENAATESDGDNVKSLDASFNEKSSFLIP
                     FECSVLNIPQDSDCFIFVQQVDNMNCEIYFVDKSKTESLSGQTVEYKTLLSSVNVEGE
                     NNNALSQVDENEKIDTNDNELKNVFENTQNKLYKISTSYDKTLKLRRFISLPNRKIC"
     gene            5459..>6312
                     /locus_tag="PLS64_06935"
     CDS             5459..>6312
                     /locus_tag="PLS64_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43591.1"
                     /translation="MFFTANSIFLVYTQSNNLEVKTVHTNLSDYENIISKYSDYVPTE
                     AQRQLESELKTVEIALQFDRTADNSNGDDLKNLGRITEQYKAENPEEYKKARALHYTK
                     QELLDKQTYLSNLVNQCKSLVDYSSFIGDMDERAQMQTKFSVFAEEGSFSYNNILKTA
                     EDFKDVNNTKLKIGNNFAVENSTTFVLTDLLVFALVFLMCIYIFTLERDKELYGLIRT
                     AKYGRMPLIISKLFVLIFFTILICAVYYSSNIALCGLYSGFGDMTRNIQSCELFMNCN
                     LKLQIWQYL"
BASE COUNT         2081 a          944 c         1172 g         2115 t
ORIGIN      
        1 aggagcaagc acttgcgttt ataaaatata ctcttagtga tgaaattcaa gaaattggtg
       61 caacagcatc aggaagtttg agttttcctg tgaatatatc tgtttacgat aatgctaagc
      121 ttgttgccgg ctcaagaacg gatgataaca ataaaattat cgggattgac aacgatttta
      181 tgaaagcata cattgacatt gccgataatg taaacgcttg caccttgtat cgagatgtgt
      241 cacattcata ttataatgac aatgttatcg gtgacatagt tgacaattat ataaataaag
      301 gtatatcaaa agataaattt attcgtcagc ttacatcggc aacggaaatt tatttgactg
      361 agtaggaata aattatgaga aaatctaaat taacaagaaa acaaaaagaa catatagcag
      421 catacctgtt tatattaccg tcttttttag gcatagcggt gttttatatt gtgccgtaca
      481 taatttgttt taccaaaagc ctgtatgtcg gcgatacttt tgtgggcata gaaaattaca
      541 tacagctttt tcaaaacaac acatttttgc ttgcacttaa aaacacgggt atatttacag
      601 ttacggcgat accgttgtta atggtgattt cgtttttgat agcgttgttt cttaattctt
      661 ttaataaaat atcttccttt ttcagaagtg cattgcttat tcctgttgta gttccgattg
      721 catctctgat ttgcgtgtgg caattagttt ttgatgatta cggtgcaatc aacggattac
      781 ttaattcact tggatttaat actgttgaat tttttaattc ggaatggtct atggtgatga
      841 tcgtttttat ttatgtgtgg aagtactgcg gattttgtgt gattcttttt acggcaggca
      901 ttgccaatgt ttcgcaatct ctctacgaaa gcgcaaggct tgacggtgca aaagctcatc
      961 agcttgtgac taaaattact atacctatga ttgcgccaac tacatttttt gtttttctga
     1021 tggagcttat ttattcattt aaaattttcc gtgaggtatt tgctttgttt ggcgattatc
     1081 caaacaccaa tgtgtatttt ttgcagaatt tcatcaacaa caattattac aatcttaact
     1141 atccaagatt gtcggcggca tcaattgtct tatcgctgtt tataattatt gtgctgttta
     1201 ttttcttctg ctttgaaagg aaacacagct attcttgaga ggtttgttat gaaagatacg
     1261 gttatgaaaa tactaaaata tattttgctg attgctgtaa ctgtgctgtt tttgtttcct
     1321 gtcatttata tgcttgcttg ttcatttatg acaggttcac aggtaagcga gatgctcaat
     1381 ttttctgacg gaaaatataa agaactcaat atgattccga atatggcaac tttggagcag
     1441 ttttatcaga ctcttttcag aaaacccgaa tatttgttaa agttctggaa ttcggttgtt
     1501 tgtacttttc cgacagtaat cggtcaggtt gcagtatcat cattggcggc ttttgctttt
     1561 gcaaaattga aattcccgtt cagggacaag ctgtttttca tatacataat tttgctcatt
     1621 ttgccgttgc aggtaacact tgttcctgat tatatgattc ttgaaaatat gaatttgctg
     1681 aataattttc ttgcggtaat tttacccgga actttttctg cattcggaat ttgtcttttg
     1741 aggcaaagta taaaatacat tcccgattcc gcaattgagg cggcaagagt tgacggtgcg
     1801 tcatatttga agatatttac taatattatt cttccgcaga ttaagggcgg actcataaca
     1861 cttgcgttgc tctgctttat tgataattgg aatgtagttg aacaaccgct gatttatttt
     1921 gataatgagg gtatgtatcc gctttccgta acgcttactg atgtcggaag ttcggattac
     1981 ggcataatat ttgcctgcgg agttatattt atgattcctg cattgcttat ttatctttac
     2041 ggacacaggg atattgattc gccgtttgtc ggaactgata agggatagga gagaggcttt
     2101 tatgaaaaaa gataagctaa agagcgtagt gttgaaattt tcaattgtat tttttattgt
     2161 gattgcatta cttacatatt tttcaaaaac aatcaataat atgcttcttc caaaggtcaa
     2221 agtggtttcg gttcaaacag gtgtaattga cgataccgca ggttcaaatg atatgaaaac
     2281 acattatctt ttgcctgttt catcggtgga tggggcaggc aatacgggaa tagtatttgt
     2341 tataaataaa accgaaaacg gtgatgcaac agttgaagag gtttcggtgg atatatgtaa
     2401 cagtgacgag ctgtattgcg aggtgacatc ggactcactt tttggcgaca gtcaagttgt
     2461 atataaaaca accaagagta tagaaaacgg ttcaagtgtt tatattgagg aggaaacagt
     2521 gtgagcaaaa gaaaaattgt ttcgtttctg ttactgctgt tgctgactgt gagcgttttt
     2581 attgcgaata ttataattat tgaaaatgca aaagggcaat tacctgatgt tactcaatta
     2641 acttatgata aaaattccga aactgctcca aaaacggttt ctgaacttga aaagctgttt
     2701 gaacggatta ctgacaaagg catagctttt tgttccgagg gtaaagaaac aaaagttaag
     2761 gaagaaaccg taactacggt acttgtgaat gaaaactggt tttcggatta taagaccgaa
     2821 attaacggcg aaaatatttc gtccaaaaac atagataaca gagataaggt tgcaataatc
     2881 ggaagttctc ttgctctcaa actgtttttt acaacagatg cagtcggtaa aaagatttgc
     2941 attgacggaa atcaatatac aatttgcggt gtgatatgcg aaaacgaaag tttaataaat
     3001 aagttttctg ctgatgacaa acagagagtg tatgttccat atacaacatc cgaaaattat
     3061 ggtgacagaa cggttgatat gattgtttac gataactcgg tatatacggg agcaattgtt
     3121 gagcaaatgg atttgccgca gtattattcg gttaatctga atgagaaaaa tcaaacgctc
     3181 tcgtcatttg aacatatttt ataccttgca atatttatcg gatttagcat tattgcattg
     3241 aagttgtggt ataggttcag tagaaaattt tttgaggaga taaaagaaaa tctcaaatta
     3301 aactactttc tgaaatcgct aaaaaatatt ccgctaaaat atgttgcact tgtgcttgtt
     3361 tttgcaggaa tacctgccgt gcttttgatt atatttaata tttgtgattt cagcattttt
     3421 atcccctcaa aatatatacc gaatgataac attttcgaca tttcaaatta cctgaatgtg
     3481 cttgtcgata atgcacaaac actaaactca caaagcctaa caggaaatca aatgcttgta
     3541 aatctgttct caagaacatt tacagcgtca ctgtggctga caataatatt tttgattagc
     3601 tggaattttg ttatatgtta ctttttgtta gactatagaa ttaaaactaa aaaataaagc
     3661 gaatttaaaa ttatagtatt ggagaatata agtatgatta accgaataaa taagataaaa
     3721 agtaaaaatc ctaaactaag gaaaattttt aaaatgaaaa aattattaag tgcgttacta
     3781 attgctttat tgttgccgac tatgatgttg atgtctgcat gtaatgatgc aagcaaaact
     3841 tcagatgata aattatcatt tgactacaca acaaatttta aatattatta cagtaacttg
     3901 aacggaataa attttcctgt gacaaaatca gaaaaagggt actatgtgtt tttgcctaat
     3961 aattaccttt actatattga ccgtgaatca caggcggcaa caccgctatg taacaagcct
     4021 aattgttcgc ataacagtgt tgattgtaat gcatacttta atttatttgt aaatgcatca
     4081 ggagaatcag ctaatgttgt gcaatattat gacggtaatt tatatattgt tgtaaaagat
     4141 gaagatgcta tgggtaattt tctcggaaat atattgtaca aagttacacc tgatggatca
     4201 acaagggaaa aactgattga atttaaagac ggcatttcgc attggctgat tcataaaggt
     4261 tatttttatt tctcacagga taagtttgcc gatgatattg attcgtcctc agtttatgac
     4321 agcttttcga taaagagata taaaataaat aacattaaat ctaagccgga ggaaatcttt
     4381 aacagcaaaa attacagtga taatcttaga ggtactttcc aatttaccgc atatgatgac
     4441 tatatttatg tgccggttct tcctatttca gaagatgaaa ttaaaaagca ggaagaaagc
     4501 ggaaaggtaa cagtttctac gtatcaggaa ttttattcga ttaatatcaa tacgataaaa
     4561 ataaaccaaa ttagaaatga tgaaggtgat gtttgctcgc cgtttttcta taatggacag
     4621 ctaacatatc tcattgataa ttcggatact aacggtaagt ttaaatattt tacaagtgaa
     4681 ctcgacggaa gcaatcccaa acttttaacg gaaatgaatt acggcgataa tttgtttgca
     4741 aatgaatcac agctgtattt acagaacttt agtgaagata cagatgaaaa cgcagcaact
     4801 gaaagtgacg gagataatgt taagtctctt gacgcttcat ttaatgaaaa aagttcgttt
     4861 ttaatacctt ttgaatgttc agtgttaaac attccccagg attcagattg cttcatattt
     4921 gttcaacagg tggataatat gaattgcgag atttattttg ttgataagtc aaaaacagaa
     4981 tctctgtcag gacaaacagt ggaatataaa acattattaa gttccgtgaa tgtcgagggt
     5041 gaaaataata acgcattaag ccaagtagat gagaatgaaa aaattgatac taatgataat
     5101 gaattgaaaa atgtttttga aaatacacaa aataagttgt ataagatttc aacatcatat
     5161 gacaaaacgc ttaaactccg cagattcatt agtttaccaa accgaaaaat ttgttaaaga
     5221 aaatcctctt tcttttgtaa atggtcaata tgtagctttt gtatctgttg ataaaattcc
     5281 cgtagaaatt aaagatatgt tagtttctat tataagaaat gaacctattt cgccaattaa
     5341 cagcactgat tttggcggag aaatctactc atttaattaa ggtcgtaaca atatgaaatt
     5401 gattatgttc gagatttaca aactgttcag caaaaaactg cttttaatat gccttgtttt
     5461 gttttttact gcaaattcaa tattcttggt ttacactcaa tcaaataatc ttgaagtaaa
     5521 aacagtacat actaatttat ctgattatga gaatataata tctaaatatt cagattatgt
     5581 gccgactgaa gcacaacgac aacttgaaag cgaattaaaa acagttgaaa tcgctcttca
     5641 atttgacaga acggcagata acagcaacgg cgatgatcta aaaaatcttg ggcgaattac
     5701 cgaacaatat aaggcggaga acccggagga atacaaaaag gcgcgggcgt tacattatac
     5761 aaaacaagag cttcttgata aacaaaccta tctttcaaat ttggtaaatc aatgcaagtc
     5821 tttagttgat tatagctctt ttataggcga tatggatgag cgcgcgcaga tgcagacaaa
     5881 gttctcggtt tttgcggaag aaggttcgtt ttcatacaac aacatattaa aaaccgccga
     5941 ggattttaaa gatgttaata atacaaagct taaaatcgga aacaattttg ccgtagaaaa
     6001 ttcaacaaca tttgttctga ccgatttgct cgtatttgcg cttgtatttt taatgtgcat
     6061 ctatattttt acattggaaa gggacaagga attatacggg cttatcagaa ccgcaaaata
     6121 cggacgtatg ccgcttatta tttcaaaatt attcgtcctt atctttttta cgattttaat
     6181 atgcgcagta tattattctt caaatattgc actctgcgga ttgtacagcg gcttcggcga
     6241 tatgacaagg aatattcagt cctgcgagct ttttatgaat tgtaatttaa agctgcaaat
     6301 ttggcaatat ct
//
LOCUS       DAOMVE010000112         6233 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3379_length_6233_cov_3.430722, whole genome shotgun sequence.
ACCESSION   DAOMVE010000112 DAOMVE010000000
VERSION     DAOMVE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3379_length_6233_cov_3.430722"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLS64_06940"
     CDS             complement(<1..235)
                     /locus_tag="PLS64_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43592.1"
                     /translation="MITVIGYILTTLGFGGLAYSLFTVNTNKRYSWTSPFSSFEKTII
                     AVAIISFIILLVGIIMVIFSFVKKKNEDKLNSLK"
     gene            390..575
                     /locus_tag="PLS64_06945"
     CDS             390..575
                     /locus_tag="PLS64_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HRL43593.1"
                     /translation="MKKFKIPQIPQTTSKSIRFPNDVIDEVESVLVGTDCTFSAFVVE
                     AVRVALENLKEESAEDE"
     gene            complement(636..2183)
                     /locus_tag="PLS64_06950"
     CDS             complement(636..2183)
                     /locus_tag="PLS64_06950"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRL43594.1"
                     /translation="METKFIGLEGFNEIESLPTKKRKVCRRELTTAQKTVRFVKRATT
                     FCAKSVVRMSKNTAAKLAQAIENRKGSLKSVTIKAVNSSKKAKTQISAIDKAFNESRN
                     GTTKSAGEIVSELSFKSGKQYAHLAPSAIRSQKLLRKKAVLAVVACFSAITLSCVTVA
                     SALDMKDNNSVQTPALVATSDEAVPHTEIDGESASANLKAISPSCASLYIDGKFIGAT
                     EEIDQLNADLDQVLVDYRRDYDDETTTEFANSVEVVTGNASGTDLITADEVMALADGK
                     FSISLSTDIVYTRDVAYDTKVKYDEDKSSSYKKVTTKGVNGEEEVTVRTTFVDGVQTD
                     AVQTDAKTLKEAVDEVVVKGKAEDTSSSTGSSSTSSNSSSSSSSNSSSSYTTGSSGMF
                     AWPLPYTHTLTSTFGTRWGRLHGGLDISAGGVYGQPIYASASGTVTFSGGDNSGYGNY
                     VIIDHGSGYTTLYGHCSSLVAVTGQYVNQGDLIGYVGSTGNSTGPHLHFEIRLNGEKM
                     DPLGYTS"
     gene            complement(2355..2810)
                     /locus_tag="PLS64_06955"
     CDS             complement(2355..2810)
                     /locus_tag="PLS64_06955"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HRL43595.1"
                     /translation="MKKIASFTINHNTLMPGVYISRIDGDITTYDMRFVKPNTPPYLP
                     NPVMHTIEHLFATYARNSKFADNVIYFGPMGCRTGFYFLVRDMSNEDALQLIKDTIKQ
                     CAEHKGEIPGTTAIECGNYLEHDLDGALKELNKYYSLLKDYTVDNLQYN"
     gene            complement(2826..3734)
                     /locus_tag="PLS64_06960"
     CDS             complement(2826..3734)
                     /locus_tag="PLS64_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRL43596.1"
                     /translation="MKKTICAVTAAMLALTSVLCGCSSNGESSSQSGSTTPATVATDT
                     TVKTTGEKIHINDSTLGEIWITELDGVPKNTLNNDNFTSDDTFKYYSENGKAASMEGI
                     DISSYSGKIDWDKVKKSGVDFVMVRIGGRGYGSDGKMYSDDSALSYIKGAKAAGLKVG
                     VYFFSQAVNNDEAIEEADYIKTLLGDETIDFPVAYDWEIIKDDDARTDSVSAAQATAC
                     AKAFCNRVKELGYTPIIYSPSKELYFKYDLTQLADFDIWYCEYANVPTFYYQFSMWQY
                     SSSGKVDGIEGDVDMNICFTNIADYD"
     gene            3987..4319
                     /locus_tag="PLS64_06965"
     CDS             3987..4319
                     /locus_tag="PLS64_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43597.1"
                     /translation="MNETKVHGYRHKTTEELVKAIDECTSLSQLFALIQHEHITIQML
                     TRPGASNLAPKILSPKEITGNRDTPFERLRKQVRESVLEDERRLKQSKLIAECERLSR
                     LNKNDKIK"
     gene            4424..4630
                     /locus_tag="PLS64_06970"
     CDS             4424..4630
                     /locus_tag="PLS64_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43598.1"
                     /translation="MTFKFKHTLAVILAVVTVACSVVGTLAVSAAETDSQPATEATVA
                     SDVTDTSVEPTQATEPSQPVTERP"
     gene            4669..5901
                     /locus_tag="PLS64_06975"
     CDS             4669..5901
                     /locus_tag="PLS64_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRL43599.1"
                     /translation="MGAIKNLKRTQYTTNSLSFKWDKVSGATGYVIYYRNNDAHKNFS
                     RFTYVKSNACTLKKIRTATWYDVKVAAYVEHDGVKYEGKSQTYRTATLPNAVSVSLKE
                     SGSAITLSWSKNSQATGYKIYRMSGDTKGRYVLYKTITSNSTTSFRDKGVKNGRAYYY
                     QVKAYRAIKKNTYYSSPSTIRCVAGLNAVNFKTDTRLSRVNLTWGKAMTTPTAYEIFY
                     STSKNGKYTKLGETKNTFYNTKKLTVGKTYYFRIRAYKYSGPSSNPKKYKCLGTFQTK
                     SVKISKNAYGVSVGGTYVEISINQQHMWYYKNGKLVVETDVVTGNYGTNDTPKGAYSI
                     IYKASPATLMNNSHVTFWLPFTSDGCGIHDASWRSSWEYGGTRYKGHGSHGCVNTPYN
                     AAKKIYNNISSGTRVVVY"
BASE COUNT         1868 a         1280 c         1274 g         1811 t
ORIGIN      
        1 ttttaagtga attaagctta tcctcatttt tctttttaac aaaagaaaat ataaccataa
       61 taattcctac aagcagaata ataaatgaaa ttatagcaac tgcaataatt gttttttcaa
      121 atgaagaaaa tggagatgtc cacgaatatc ttttgtttgt attcacagta aataacgaat
      181 atgcaagtcc gccaaagccg agagttgtca gaatataccc aataacagta atcacttatt
      241 gcctccttat taaatttttt agatatgaag cttcattgat tcaattataa cattgcgtaa
      301 tacaaaatgc aagacaaaag taatacgcaa tttttgtgta aattgcatac aatagtaata
      361 tataatgtaa tacaggagca gttcacgcaa tgaaaaagtt taaaattccg caaattcccc
      421 aaacaacaag caaaagcata cgatttccaa acgatgtgat tgatgaagtg gaatctgtcc
      481 ttgtcggtac ggattgcaca ttttctgcct ttgttgtgga agcggtaagg gtagctcttg
      541 aaaatttaaa agaagaatca gccgaagatg agtaaacaaa accccctatt caagtgaata
      601 gggggttata attttgcatt ttatatttaa ttaaatcagc ttgtgtagcc gagggggtcc
      661 attttttcgc cgttgaggcg gatttcaaaa tggaggtgag ggcctgttga gttgcctgtt
      721 gaaccgacat aaccgatgag gtcaccctga tttacatact gacctgttac tgcaacaagt
      781 gaactgcaat gaccgtaaag tgttgtgtag ccactgccgt ggtcaatgat aacatagttg
      841 ccgtagccac tgttgtcacc gcccgagaat gttacggtac cgcttgcgga tgcataaata
      901 ggctgtccgt aaacaccgcc ggctgaaatg tcaagtccgc cgtgaagtct gccccagcgt
      961 gtgccgaagg tacttgtcaa tgtatgtgtg taggggagtg gccatgcaaa catacccgat
     1021 gaacctgttg tgtagcttga agatgaattt gaacttgatg aagaactgct gtttgatgag
     1081 gttgacgatg aacctgtgct tgatgaagta tcctcagcct tgccttttac aacaacttcg
     1141 tcaactgcct ctttaagagt ttttgcgtca gtctgaactg catcggtctg aacaccgtca
     1201 acaaatgtag ttcttaccgt tacttcttct tcaccattaa cgccctttgt ggtaactttt
     1261 ttgtatgaag aagacttgtc ctcgtcatac tttacctttg tgtcataagc cacatctctt
     1321 gtgtaaacaa tgtctgttga aagtgagatt gaaaacttgc cgtctgcaag tgccataacc
     1381 tcgtctgcgg taataaggtc tgtaccgctt gcgttacctg ttacaacctc caccgaattt
     1441 gcaaactcgg ttgttgtttc gtcatcgtaa tctctgcggt aatcaacaag aacctgatcg
     1501 aggtctgcat tgagctgatc aatttcttct gtcgcaccga tgaacttgcc gtcaatataa
     1561 agacttgcac atgacggtga aattgccttg aggtttgccg atgccgattc gccgtcaatt
     1621 tcagtatgag gaacagcctc gtcacttgtt gcaacaagtg caggtgtctg tacgctgttg
     1681 ttatctttca tatcaagtgc gcttgcaacc gttacacatg aaagtgtgat tgcactgaaa
     1741 caggctacta ctgcaagcac agcctttttt ctgagcaact tctgcgaacg gattgccgac
     1801 ggtgcaaggt gagcatactg ctttccgctt ttgaacgaaa gttccgatac aatctctccg
     1861 gcactctttg ttgtgccgtt tcttgattcg ttgaatgctt tgtcaatagc tgatatctgt
     1921 gtttttgctt tctttgacga attaacagcc ttgattgtaa cagactttaa agaacccttc
     1981 ctgttctcaa ttgcctgtgc gagttttgcg gctgtgtttt ttgacattct tacaactgat
     2041 tttgcacaaa atgttgttgc tctctttaca aagcgaacag ttttctgtgc tgttgtaagc
     2101 tcacgcctgc aaactttgcg tttctttgtc ggaagactct cgatttcatt gaaaccttcc
     2161 aaaccaatga actttgtctc caaaataatt tctcctatct cattaaccgt tacatttttg
     2221 taacaaacac gataccttta ttataccaaa tagttacgaa attgcaatat gagaattaaa
     2281 aaatgtcata attaataata agacagggga taaggttaca aaaaacaggg taaattaaac
     2341 aataattccc tatattaatt atactgtaag ttatcaaccg tgtaatcttt taacaaagag
     2401 taatatttat ttaactcttt taaagcaccg tcaaggtcgt gttcaaggta gtttccgcac
     2461 tcaattgcag ttgttccggg gatttccccc ttgtgctccg cacactgctt tattgtgtct
     2521 tttataagct gtaatgcgtc ctcatttgac atatcacgaa cgagaaaata aaaacccgtt
     2581 ctgcatccca taggtccaaa gtagattaca ttgtcggcaa atttgctgtt gcgggcatat
     2641 gttgcaaaaa ggtgttcaat tgtgtgcata acgggattcg gaagatacgg cggtgtgttt
     2701 ggctttacaa aacgcatatc gtatgttgta atatctccgt caattcttga gatgtaaaca
     2761 cccggcataa gtgtgttgtg gttgattgta aagcttgcta tctttttcat atatattatc
     2821 ctttcttaat cgtaatcggc tatgtttgtg aagcagatgt tcatatcaac atcgccctca
     2881 attccgtcaa ccttgcccga tgaggaatat tgccacatag aaaactgata atagaatgtc
     2941 ggaacatttg cgtattcgca ataccagatg tcaaaatctg caagctgtgt gaggtcatat
     3001 ttaaagtaaa gctccttgct cggagaatag ataatcggtg tgtagccaag ctcctttaca
     3061 cggttgcaga atgcctttgc acaggctgtt gcctgagcgg cggaaacgct gtcggttctt
     3121 gcgtcatcat ccttgatgat ttcccagtca tatgcaacgg gaaaatcgat tgtttcatcg
     3181 ccgagaagtg tctttatata gtcagcctct tcaattgcct catcgttatt gacagcctga
     3241 gagaagaaat acacgccgac tttaagtcct gccgccttag caccctttat atatgaaagg
     3301 gcggaatcgt ccgagtacat ttttccgtca gaaccgtagc ctctgccgcc gatgcgaacc
     3361 ataacaaaat caacacccga ttttttaacc ttgtcccagt caatctttcc gctgtaggag
     3421 gaaatgtcga ttccttccat tgaagcggcc ttgccgtttt cactgtagta tttgaaagtg
     3481 tcgtcagagg tgaagttatc gttgttgagt gtatttttcg gtacaccgtc aagctcggta
     3541 atccagattt caccgagagt gctgtcgttt atatgtattt tttcgcctgt tgtcttgacg
     3601 gttgtgtctg tggcaacagt tgccggggtt gtactgcccg actgcgaact gctttcaccg
     3661 ttgcttgagc aaccgcacaa aaccgaggta agtgcaagca tggcagccgt tacggcacaa
     3721 atagtttttt tcattattct actcctgaca aaaatttttt aacgctgaaa aatgtcgatt
     3781 atacggacat tttgcgtatc tgttagtcag catatcataa aaaagtgttg atgtaaacaa
     3841 agaaaccgaa aataaatatt gaaaatcttg tgaaaattaa gtataatcta aggtacaagt
     3901 gtccttcatc aaacagcgga ggcatttatt cggaaatata atgataacaa tgttccgcaa
     3961 aaggcgataa agggcggtga gtgaaattga atgaaacaaa ggtgcacgga taccgtcaca
     4021 aaacgacgga agaactcgtc aaggcaattg acgaatgtac ctctctcagt cagcttttcg
     4081 ctctcattca gcatgagcat atcaccattc agatgctcac aagaccgggt gcatcaaatc
     4141 ttgcaccaaa aattttaagc ccgaaagaga taacaggcaa ccgtgacaca ccatttgaaa
     4201 gactgcgaaa acaggttcgt gaatctgtgc ttgaggacga aagaaggctt aaacagagca
     4261 agctgattgc cgaatgcgaa cgcctttcaa gacttaataa aaatgacaag ataaagtaag
     4321 agtaattagt ggaagatttt tgaaattttt tactaaatac tctttatttt tttcaaaaaa
     4381 acatatataa tctaattatc aaatacgatt ggggatggaa tatatgacat tcaaattcaa
     4441 acacaccttg gctgtaattc tcgcagtagt taccgtggct tgttctgttg tcggaacatt
     4501 agctgtttct gccgcagaaa cagactctca gcctgcaaca gaggcaactg ttgcatctga
     4561 tgtaacagat acgagcgttg aaccaactca ggcaaccgag ccttcacagc cggtaacgga
     4621 gcgcccgtaa ctcctacggt tcctccgact cagccggtta agccgaatgt gggcgcaatc
     4681 aagaatctca aaagaacgca atatacaaca aattcgcttt cgttcaagtg ggataaagta
     4741 agcggtgcaa caggctatgt aatctactac cgcaacaatg acgcacacaa gaatttttca
     4801 agatttacat atgtaaagtc aaatgcctgt acactcaaaa agattagaac agcaacctgg
     4861 tatgatgtaa aggttgccgc ctatgttgag catgacggtg taaagtatga gggtaagagt
     4921 cagacctaca gaacagctac tctcccgaat gccgtaagcg tttcactcaa ggagtcgggt
     4981 tctgcaatta cactttcgtg gtcaaagaac tcacaggcaa caggctacaa aatctaccgt
     5041 atgagcggtg acacaaaggg caggtatgtt ctttacaaga caatcacttc aaattccaca
     5101 acttctttca gagataaggg agttaagaac ggcagagcgt attactatca ggttaaggct
     5161 taccgtgcaa tcaaaaagaa cacttactat tcgtcaccgt caacaataag atgtgtggca
     5221 ggtcttaatg ccgttaattt caagacagat acaagacttt caagagtaaa tctcacatgg
     5281 ggcaaggcaa tgaccactcc gacagcatat gagattttct attcaacctc aaagaacgga
     5341 aaatatacaa agcttggtga aacaaagaat actttctaca acaccaaaaa gcttacagtc
     5401 ggcaagacct attacttccg tattcgtgct tacaagtatt cgggaccgag cagtaacccg
     5461 aaaaagtaca agtgcctcgg cacattccag acaaagagtg taaagatcag caagaatgcc
     5521 tacggtgttt cagtcggcgg cacatatgtt gaaatcagta tcaatcagca gcatatgtgg
     5581 tactacaaga acggtaagct tgttgttgaa acagatgttg taaccggcaa ctacggtaca
     5641 aacgatacgc caaagggtgc atattcaatc atctacaagg catcgcctgc aactttgatg
     5701 aataactctc atgtaacatt ctggctaccg ttcacatctg acggttgcgg aatccatgac
     5761 gcatcatggc gttcaagctg ggaatacggc ggaacaaggt acaagggtca cggctcacac
     5821 ggctgtgtaa acacaccgta caacgcagct aagaaaatct ataacaacat ttcctcagga
     5881 acccgtgttg ttgtttattg attttaatcc gtagcgaaaa cttgataaaa taatttaagg
     5941 ctgacagatt tttaataacc tgtcagcctg atttttatat gaaagttatt gttaatcagt
     6001 aggggcgttc atcggacgcc cgctttgatg aaattatttg tttattcaca taatttttga
     6061 tgtgatgaaa actgtgtata atctgtagcg gcggtcagtg accgccaatc gtaccgattg
     6121 taaaatcggt gcgacttaac acaaattatg cccgagcgta aaattgttac cttttctaca
     6181 atacacacga aatgaataag taatcgcaga aaatattttg tttattaata tat
//
LOCUS       DAOMVE010000113         6202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3394_length_6202_cov_3.981292, whole genome shotgun sequence.
ACCESSION   DAOMVE010000113 DAOMVE010000000
VERSION     DAOMVE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3394_length_6202_cov_3.981292"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..3975)
                     /locus_tag="PLS64_06980"
     CDS             complement(208..3975)
                     /locus_tag="PLS64_06980"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HRL43600.1"
                     /translation="MSEVIRIFVEKKDGFNVLAKQTLWDIRHNLGMKSVEDLRYIVRY
                     DLEGLTKEEYDKAKTIVLSEPNADVIYEETLPVEDGWKVFAMEYLPGQYDQRGDSAEQ
                     CVQLLTQGERCKVLTARVIALKGNITDEELVKVEEYLINPVESRLASLEKPKTLDMEI
                     PVPENVKRVEGFNGWNDDEMQKYYDSMGFAMTLADLKFCRDYFRDTEHRDPSVTELRV
                     IDTYWSDHCRHTTFLTRLEEIEIEKSALGKVIEDALSEYYATRDEVYGKDTKRIVSLM
                     DMALIGMKSLKKKGLIPDLDESEEINACSIQVPVTIDGKTEQWLVQFKNETHNHPTEI
                     EPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSGDPTIPFKDTMHGKLPSRKITTGAA
                     QGYSSYGNQIGLATGQVTELYDQGYVAKRMEIGAVIGASPKENVIRETPLPDDVIVLL
                     GGRTGRDGCGGATGSSKAHDENSIETCGAEVQKGNPPTERKIQRLFRNPETAKLIKRC
                     NDFGAGGVCVAIGELADGLTVDLDKVTKKYDGLDGTELAISESQERMAVVLDKKDVDK
                     FISLASKENLEATAVAVVTESPRLTMNWRGDTIVDLSREFLNTNGVTQVAKAYIEAPK
                     WEGCYRKVAPAKLKDMPADEAFLENMSRLEVCSQIGLAERFDASIGAATVIMPFGGKN
                     QLTPQEAMAAKIPLEKGETDDATAMSYGYIPGVSRWSPFHGSVYAVVESLSKLLAIGA
                     NPMTARLTFQEYFERLKDVPSRWGKPAAALLGAMQAQLKLGLPSIGGKDSMSGTFEDI
                     DVPPTLVSFAVAMTKASKTISTEFKNAGSKVIFVPVPENKETLMPVWDKLIEMYNAVY
                     ALCEDGKVLSASVVKEGGTAASVCKACFGNGFGFKFANELTNDELFAPLSGSLVIELA
                     DGAELSNDVLHYDLGTVTNDAKITVNGKEIELSALLEKWTAPLEKVFPTKAEVPEIEV
                     DVPLYSERNTSSPAIKTAKPTVFIPVFPGTNCEVDTARAFEKAGANVEMLIVKNLSSN
                     DIEETIDEMEKLIAKSQMIMLPGGFSGGDEPDGSGKFIATTFRNPRIAEQVNNLLKNR
                     DGLMLGICNGFQALIKLGLVPYGEIRELKANDPTLTFNTIGRHISHMAYTRVTSVKSP
                     WFANVNAGDVFAVPVSHGEGRFMADVETVKELAKNGQIATQYVDLAGNPSSDIEFNLN
                     GSVCAIEGITSPDGRVLGKMGHSERKGENLYKNVPFAKDQQIFESGVKYFK"
     gene            complement(4179..4889)
                     /locus_tag="PLS64_06985"
     CDS             complement(4179..4889)
                     /locus_tag="PLS64_06985"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL43601.1"
                     /translation="MYKNYLFDLDGTLLPMDMEKFVKLYFSSLCKRCTPVIKIEPDAL
                     IKAMWAGTDAMTKNDNSRTNREVFWETAGKVSGADLEKFDDLFTDYYENEFIVPKDAT
                     SFTPYAKKSIDFIKKNGGRLIAATNPIFPFVATKRRLEWAGVSPDDFELVTTYENFGC
                     CKPNLKYFEEICKKQDIKPEDSIMIGNDVDEDLCSAKLGFDTYLITDTIVNRDNKDYS
                     DYKNGSFEEFYKDFLKGD"
     gene            5002..5949
                     /locus_tag="PLS64_06990"
     CDS             5002..5949
                     /locus_tag="PLS64_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRL43602.1"
                     /translation="MKIVLTDAQTVLDDIVNADVLNRFGEVESFGLLKYEEVAEKIAD
                     ADIVVCNKTLLDSHTMRLAKNLKYIGLFATGYNNIDLDYCTKNGITVCNAGSYSTNAV
                     AQHTFALILEHFNHTAEYNRFVQDGKWKRSKTFSPFVYPLNELAGKTLGIVGLGNIGK
                     AVAKIANAFDMNVIASSRTPKNIDGIKDVSFDELLETSDIVSVHCPLNPQSENMFDKN
                     AFANMKQGALFVNTARGGVMVEEDLLEALESGHLGGAAIDTLKVEPMVEDCILMGAKN
                     CIITPHIAWAPVETRERLMGIVSDNIKNFLNGTPTNKVN"
BASE COUNT         1720 a         1398 c         1320 g         1764 t
ORIGIN      
        1 agttttacaa atgcaaagct ataaatgccc gagcgtaaca ttgccgatta tattttaatg
       61 tcacaatgct actgccatac agtagtggcg aacctcgttc gccgctacaa taacaataca
      121 atattattgc gtactatacc gcacaaacaa caaggacatc cgaaaaacgg atgtccttag
      181 tttttatata aaatattcgg ttataaatta cttgaagtat tttacgccgc tttcaaaaat
      241 ctgctggtct tttgcaaaag gaacattttt gtaaaggttt tcgccctttc tctcgctgtg
      301 tcccatttta ccgagaactc tgccgtcagg tgatgtgata ccctcaattg cacaaacaga
      361 accgttgagg ttgaactcaa tgtcgctgct tgggttgccg gcaaggtcaa catactgtgt
      421 tgcaatctga ccgttctttg caagttcttt tacagtttca acatcagcca taaatctgcc
      481 ctcaccgtgt gacacgggaa cagcaaacac atcgcctgcg ttgacatttg caaaccacgg
      541 tgacttaacg gaagtaactc ttgtataagc catatgtgaa atatgtctgc cgattgtgtt
      601 gaatgtgagc gtcggatcat ttgctttaag ctctctgatt tcgccgtaag gaacaagtcc
      661 gagcttgata agagcctgga atccgttaca gataccgagc ataagaccgt cacgattctt
      721 gagaaggttg ttaacctgct ctgcaattct cgggttgcgg aatgttgttg caatgaactt
      781 acctgaaccg tcaggctcgt caccgcctga gaaaccgccc ggaagcataa tcatctgtga
      841 ctttgcaatg agcttttcca tttcgtcaat tgtttcctcg atatcgtttg aagaaaggtt
      901 cttgacgatg agcatttcaa catttgcacc tgctttttca aatgctcttg ccgtatcaac
      961 ctcgcagttt gtacccggga atacagggat aaatacagta ggctttgcag ttttgattgc
     1021 aggtgaagat gtatttctct cgctgtaaag cggaacatca acttcgattt caggcacttc
     1081 agcctttgtc gggaatacct tttcaagcgg agcagtccac ttttcgagga gtgcggaaag
     1141 ttcgatttcc ttgccgttta ccgtaatctt tgcatcgttc gtaacagtac cgaggtcata
     1201 gtgaagaaca tcgtttgaaa gctctgcacc gtctgcaagt tcgataacaa gcgaacccga
     1261 aagcggagcg aaaagctcgt catttgtgag ttcgtttgca aacttaaagc caaagccgtt
     1321 accgaagcaa gccttacata cgcttgcggc agtaccgccc tctttaacaa ccgatgcaga
     1381 aagcaccttg ccgtcctcgc agagagcata aacagcgttg tacatttcga taagcttatc
     1441 ccatacaggc ataagtgttt ctttattttc gggaacggga acaaagataa cctttgaacc
     1501 tgcgttcttg aattctgttg aaattgtctt tgatgccttt gtcattgcaa cggcgaaact
     1561 tacgagtgta ggcggaacat caatatcctc aaatgtaccc gacatactgt ccttaccgcc
     1621 gattgacgga agtccgagct tgagttgagc ctgcatagca ccgaggagtg ctgcggcagg
     1681 tttaccccaa cgggaaggaa catctttaag tctttcaaaa tattcctgga atgtaagtct
     1741 tgctgtcatc gggtttgcgc cgattgcaag cagctttgaa agcgattcaa caactgcata
     1801 aacagagccg tggaacggac tccagcgtga aacaccgggg atatagccgt aactcattgc
     1861 tgttgcatcg tctgtttcgc ccttctcaag cggaatcttt gcagccatag cctcctgagg
     1921 agtgagctga ttcttaccgc cgaacggcat aattaccgta gccgcaccga ttgatgcgtc
     1981 aaatctttct gcaagaccga tctgtgagca aacttcaagt cttgacatat tttcaaggaa
     2041 tgcctcgtca gcaggcatat ctttaagctt tgcgggagca accttacggt agcagccttc
     2101 ccacttcgga gcctcgatat aagccttggc aacctgagta acaccgtttg tgttgaggaa
     2161 ctcacggctg aggtcaacga ttgtgtcgcc tctccagttc attgtaagac gaggactttc
     2221 ggttacaacc gcaacggctg tagcctcaag gttttctttt gatgcaagtg aaataaactt
     2281 gtcaacatct tttttatcga gaacaacagc cattctttcc tgactttcgg atatagcaag
     2341 ctccgtaccg tcaaggccgt catatttctt tgttaccttg tcgaggtcaa ccgtaagacc
     2401 gtcggcaagt tcaccgatag caacgcaaac accgcctgca ccgaagtcgt tacatctctt
     2461 gatgagcttt gcagtttcgg ggttgcggaa caaacgctga atttttcttt ctgtcggagg
     2521 attacccttc tgaacctctg caccgcaggt ttcgattgag ttttcatcgt gagcctttga
     2581 agaacctgtt gcaccgccgc agccgtcacg gcctgttctt ccgccgagaa gtacgattac
     2641 atcatcgggg agaggagttt cacggataac attttccttt ggagatgcac cgataacagc
     2701 accgatttcc attctctttg caacatagcc ctgatcgtaa agctcggtta cctgacctgt
     2761 tgcaagaccg atctggttac cgtatgagct gtaaccctga gcggcacctg ttgtaatttt
     2821 gcgtgaaggg agcttgccgt gcattgtatc cttgaaagga attgtcggat cgcccgagcc
     2881 tgtaacacgc atagcctgat atacatatga acggcctgaa agcggatcac ggattgcacc
     2941 gccaagacag gttgcggcac cgccgaaagg ctcgatttct gtcgggtggt tgtgagtttc
     3001 gttcttgaac tgaacgagcc actgttctgt ttttccatct atagtaacgg gaacctgaat
     3061 tgagcaggca ttgatttctt cactttcgtc aaggtcggga atgagtccct ttttcttgag
     3121 tgacttcata ccgatgagag ccatatccat aagtgaaacg attctctttg tatccttgcc
     3181 gtaaacctcg tcacgggttg cataatactc gctgagtgcg tcctcgataa ccttgccgag
     3241 agcagacttt tcaatctcaa tctcttcaag tcttgtaagg aatgttgtgt gacggcaatg
     3301 gtccgaccag tatgtgtcaa ttacacgaag ttctgtaacc gacggatcac ggtgctctgt
     3361 atctctgaag taatcacggc agaacttgag gtctgcaagt gtcattgcaa agcccattga
     3421 atcgtaatac ttctgcatct catcgtcatt ccagccgttg aagccctcta ccctcttaac
     3481 attttcggga acgggaattt ccatatcaag agtctttggc ttttcaagag atgcgagacg
     3541 gctttcaaca gggttgatga ggtactcctc aacctttaca agctcttcat ctgtaatatt
     3601 gcccttgaga gcaattactc ttgctgtaag aaccttgcaa cgctcaccct gagtaagaag
     3661 ctgaacgcac tgctcggcag agtcaccgcg ctgatcgtac tgaccgggga gatactccat
     3721 tgcgaatacc ttccagccgt cctcaacggg gagagtttct tcatagatta catcggcgtt
     3781 aggctctgac aaaacaatgg ttttcgcctt gtcatattct tccttagtaa gtccctcaag
     3841 atcgtaacgc acaatgtaac gaagatcttc gacagacttc ataccgaggt tgtggcggat
     3901 gtcccaaagc gtctgctttg caagaacatt gaagccatcc tttttttcaa caaaaattct
     3961 gattacttct gacataattc attcacctgc ttaattaaga tataaaacaa aataaatccc
     4021 gtctgataac ctgtatcaca tcacataata ccacattcga ctattttttt atttcattta
     4081 aggcattaaa aggtattctg taaaagtaaa attaaatgca cacagaaaaa gcggtcagac
     4141 aaagcaaaat gccgtctgac cgcacaatat aatcatattt aatcgccttt gagaaaatct
     4201 ttgtaaaatt cctcaaaact gccgtttttg tagtctgaat aatccttatt atctctgttc
     4261 acaattgtgt cggtaataag gtatgtatca aagccgagtt ttgcgctgca caaatcctcg
     4321 tcaacatcgt tgccgatcat aatgctgtct tcgggcttga tatcctgctt tttgcagatt
     4381 tcttcaaaat atttcagatt cggcttgcag caaccaaagt tttcgtatgt tgtaacaagt
     4441 tcaaaatcgt cgggagaaac tcctgcccat tcaagtctgc gttttgttgc aacaaacgga
     4501 aaaatcggat ttgttgcggc aataagtctg ccgccgtttt tctttatgaa atcaatactt
     4561 ttctttgcat aaggagtaaa gcttgtagca tctttcggaa cgataaactc gttttcgtaa
     4621 taatcggtaa aaaggtcatc aaacttttca aggtcggcac cgctgacttt gcccgctgtt
     4681 tcccagaaaa cttcacggtt tgtacggctg ttatcgttct ttgtcatagc gtcagtacct
     4741 gcccacatcg cctttataag ggcgtcgggt tcgattttta taacaggagt gcaacgcttg
     4801 cagagcgatg aaaaatacag ctttacgaat ttttccatat ccatgggaag caaagttccg
     4861 tccaaatcaa aaagataatt tttatacatt aacataccgt tcctttcaat ttatgcacaa
     4921 atattataca aaatttcgat ttggattgca aggaaaaaat tttcggtata tactatagtc
     4981 agtaaaaaac gaggtgataa gatgaaaata gttttaacag acgcacagac tgttcttgac
     5041 gatattgtaa atgccgatgt gcttaatcgg ttcggcgagg ttgagtcgtt cggtcttttg
     5101 aaatatgaag aggttgcgga gaaaattgca gatgcagata tcgttgtgtg caacaaaact
     5161 ctgcttgatt cgcacacaat gcgacttgca aaaaatctga aatatatagg actttttgca
     5221 acaggctaca acaacattga ccttgactac tgcaccaaaa acggcataac cgtctgcaac
     5281 gcaggttcat attcaacaaa tgcggttgca cagcacacat ttgcactcat tcttgaacat
     5341 ttcaatcaca ctgcggaata taatcggttt gttcaggacg gcaagtggaa aagaagcaaa
     5401 actttttcac cgtttgtata tccgctgaat gaacttgcag gcaaaacact cggcattgtc
     5461 ggactcggaa acatcggaaa ggctgttgca aaaattgcaa acgcttttga tatgaatgta
     5521 atcgcatcga gcagaactcc gaaaaatatt gacggaatca aagatgtttc atttgacgaa
     5581 cttcttgaaa caagcgacat tgtaagcgtt cattgtccgc taaatccaca gtcggaaaat
     5641 atgtttgaca aaaatgcctt tgccaacatg aaacagggtg cattgttcgt aaacacggca
     5701 agaggcggtg ttatggttga agaagatttg cttgaggcac tcgaaagcgg tcacctcggc
     5761 ggtgcggcaa tcgacaccct caaggttgag cctatggttg aggactgcat tcttatgggt
     5821 gcaaaaaatt gcatcatcac cccccacatc gcatgggcgc ccgttgaaac cagagaacgc
     5881 cttatgggca tagtttcgga caacatcaaa aattttctga acggaactcc gacaaataaa
     5941 gtaaattaac ggcgagtaag taatgacaca aattatatat taagatatat tttataaatg
     6001 tcacaatgta attgacctac tgtagcggcg aacgaggttc gccaatcatt tcgtgtgcaa
     6061 acacgaaatg aataagtaaa tgtacaaatt tttgagcaat aaaattacta atgtaaatat
     6121 aaaaatccga cagaaacaac cgcccacctt tcggtgagcg gttgttttgt gtagtgtagt
     6181 attaggaaat tgggattaga tt
//
LOCUS       DAOMVE010000114         6179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3424_length_6179_cov_3.406760, whole genome shotgun sequence.
ACCESSION   DAOMVE010000114 DAOMVE010000000
VERSION     DAOMVE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3424_length_6179_cov_3.406760"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLS64_06995"
     CDS             complement(<1..246)
                     /locus_tag="PLS64_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43603.1"
                     /translation="MKKIISIILLAVIISTSVLFTGCGEVASNNAQTSDEADTTALSD
                     TTPTADPNQFSVKFYDYKTNELISKSNKNNLNEKMRAS"
     gene            complement(381..2846)
                     /locus_tag="PLS64_07000"
     CDS             complement(381..2846)
                     /locus_tag="PLS64_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRL43604.1"
                     /translation="MYQFKGFTEKANKALNLAIESAEEMRHNYVGTEHILYGLVKEGS
                     GVAATALNECGVTEDALREKLESINGTMSLVELTPDDFTPRTKRVLRAAVIISSKTGY
                     TYVGTEHLLLAILSESDSYAVAFLEELGVSVERLAQAVSKGMQGGADDGFGGFENESA
                     PNGSQKGGSALDKFGRDLTQAAKNGEIDPVIGREKEIQRVIQILSRRTKNNPVLIGEP
                     GVGKTAVAEGLALEIAKGNVPEILKDKRVVSLDLTGMVAGAKYRGDFEERIKAAIDEV
                     KKSKNTILFIDELHTIVGAGAAEGSADAANILKPSLARGDFQVIGATTLNEYRKYIEK
                     DAALERRFQPVKVGEPTPEQAVQILKGLRDSYEAHHKVKITDEAINAAVTLSSRYIAD
                     RYLPDKAIDLIDEGASKVRLASLTSPDNVKELEDEIADYEKEKASAINEQDFERAARL
                     RDEQKELQTKLDDAKKKWQEQQKGNSGEVTAEDIAKIVSEWTGIPVVQLTKEESERLL
                     NMENVLHERVIGQSEAVTAIAKAIRRGRVGLKDPKRPVGSFIFLGPTGVGKTELCKAL
                     AEAMFGDENAMLRLDMSEYMEKHTVSKLIGSPPGYVGFEEGGQLTEKVRRKPYSVVLF
                     DEIEKAHPDVFNMLLQILEDGRLTDSQGRTVDFKNTIIIMTSNVGARLITEKQSSLGF
                     NSENENAEESEKKDIKELVTGELRKVFRPEFLNRVDDIIVFNKLNKDEIKQIAVKMLK
                     TLENRLDKMNIKISFTDNAISEIADKGFDENYGARPLRRAIQNEIEDPLSEQMLEGKV
                     KDGAVVTCDFADGQFTFTTANAN"
     gene            complement(2893..3882)
                     /locus_tag="PLS64_07005"
     CDS             complement(2893..3882)
                     /locus_tag="PLS64_07005"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HRL43605.1"
                     /translation="MSNEVISTRVRLARNLKDYPFPCRLSEQGRKKVIEKVTSAIRDS
                     NSSIASDFNLIKLDDLTEAQGVSLVERHLVSPEFISETEGRALLLSKDESMSIMINEE
                     DHIRLQVITDGLSLEQAYDTADKLDTLLDENLEFAFDDKLGYLTQCPTNLGTGMRASV
                     MLHLPALEKSRTIGRIAGNLSKLGLTIRGAYGEGSEPSGSLYQLSNQVTLGISEKAAI
                     ENLENITKQLVSQERQARERLAKSIDIQDSVSRSLGLLKSAMVMTHDEALKLLSNVRF
                     GILSGQIKDVTAEVVDSLMEKIEPATLMVNSGKNLSAQERDIERAKILREALS"
     gene            complement(3885..4388)
                     /locus_tag="PLS64_07010"
     CDS             complement(3885..4388)
                     /locus_tag="PLS64_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRL43606.1"
                     /translation="MKCQKCGANNANTHVKTVINGEIKEYDLCSECAKKMGYTNIFGN
                     FEDEFSGLLGSFFGNSLPARTQATRCDFCGSSYSDIAKSGHVGCAKCYEIFGDQLLPS
                     IRRIHGNTTHCGKNSRFGTKKAEKSAEMTKKDKIKALKKELDKAISEQNFEHAAELRD
                     QIKEMEA"
     gene            complement(4410..4859)
                     /locus_tag="PLS64_07015"
     CDS             complement(4410..4859)
                     /locus_tag="PLS64_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HRL43607.1"
                     /translation="MRMSDLVAQYIIEMLDRENGSAEIQRNELAGNLGCVPSQINYVI
                     TSRFTPEKGYIVESRRGGGGFIRISRVKMDRGTALMHIINSIGTTLDKASAEAMLKNM
                     LQRDMIELTSARLIASALSDRTLTNVEQSKRDAVRADLFKNMLLTLS"
     gene            complement(5071..5499)
                     /locus_tag="PLS64_07020"
     CDS             complement(5071..5499)
                     /locus_tag="PLS64_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43608.1"
                     /translation="MTIEKIKRIYASTAIYKFSQGLKNAETDDFDSAFDNVLDFFGNK
                     IEEDALKIECSGDEVNMMFLDFVRMLSSTRHLAMCVFVNGNADVDTVKRLERFSGWSE
                     CFKVVRDMNKHDDKICIYIGFINWFDGEIYDVDYTVNVIM"
     gene            5721..>6179
                     /locus_tag="PLS64_07025"
     CDS             5721..>6179
                     /locus_tag="PLS64_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43609.1"
                     /translation="MYSDFALRTVLYTILCIGAIGISTGKLFAINHGISKYLAKKSYS
                     GNFKTVKTTFISLGVEYVSIAIIALFISLCTINTIYTNAFFDELLPEFFSTAVTVFAI
                     VVLGAECDFGLWLLFAIPYTFLIIANILILFFINYKFVYKDFEVPKIKKMK"
BASE COUNT         1591 a         1413 c         1168 g         2007 t
ORIGIN      
        1 acttgccctc atcttttcat taaggttatt tttgtttgac tttgaaataa gttcattagt
       61 tttgtagtca taaaatttaa ctgaaaattg gtttggatct gctgtcggag tagtatctga
      121 taaagcagtt gtatcggctt catcagaagt ctgagcgtta tttgatgcaa cctcaccaca
      181 acctgtaaat aaaacagaag ttgagataat aactgccaac aagataatcg agataatttt
      241 tttcataatt aacaccctct tgtgtttttg tcggcttaat tatacacctc aaattcattt
      301 tgtcaagata ttcaaaacaa aaaaatcaac cgcaaagttt ttcactctgc ggttgatttt
      361 atttttatta aggattacgc ttaattggcg tttgctgttg taaaggtaaa ctgaccgtct
      421 gcgaaatcgc aggtaactac tgcgccgtcc ttgaccttgc cctcgagcat ctgctctgaa
      481 agcggatctt caatctcgtt ctgtattgca cggcggagag gtcttgcacc gtagttttcg
      541 tcaaagccct tgtcggcaat ctcgctgatt gcgttgtctg taaagctgat tttaatattc
      601 atcttatcaa gacggttttc aagagttttg agcatcttaa cggcaatctg cttaatttcg
      661 tccttgttga gcttgttgaa cacgataata tcgtcaacac ggttcaagaa ttcaggacgg
      721 aataccttgc ggagttcgcc tgttacaagc tccttgatgt cctttttctc gctctcttcg
      781 gcattctcat tttcggaatt aaaaccgagt gagctctgct tttcggtgat aagtcttgcg
      841 ccgacattag aggtcattat gataatggtg ttcttaaagt caaccgttct gccctgtgag
      901 tcggtaagtc tgccgtcctc aagaatctgg aggagcatat tgaatacatc ggggtgagcc
      961 ttttcaatct catcaaaaag tacaaccgaa tacggttttc ttcttacctt ttcggtgagc
     1021 tgaccgccct cttcaaagcc tacatatccg ggcggtgagc cgataagctt tgacacggtg
     1081 tgtttctcca tatactcact catatcaaga cggagcattg cgttttcatc gccgaacatt
     1141 gcctctgcaa gagccttgca aagttctgtt ttaccgacac ccgtcggacc gaggaagata
     1201 aacgaaccga caggtctttt cgggtcttta agacctactc tgcctcgtct gatagccttt
     1261 gcgattgcag ttacagcctc gctctgaccg ataactcttt cgtggagaac attctccatg
     1321 ttaagaagtc tttcgctttc ttccttagta agctgaacaa cagggatacc tgtccattcg
     1381 gaaacaatct ttgcgatatc ctcggcagtt acctcgccgc tgttaccctt ctgctgttcc
     1441 tgccacttct tctttgcatc gtcaagcttt gtctgaagtt ccttctgctc atcacgaagt
     1501 cttgcagccc tttcaaagtc ctgctcgtta attgcgcttg ccttttcttt ttcatagtct
     1561 gcaatttcat cttcaagctc ctttacatta tcaggagagg taagagatgc aagccttacc
     1621 tttgacgcac cctcatcaat aaggtcaatt gccttatcgg ggaggtaacg gtctgcgata
     1681 tatctcgatg acaatgttac tgcggcattg attgcctcat cggtaatctt aaccttatgg
     1741 tgtgcctcgt agctgtcacg caagcctttg agaatctgta cagcctgttc gggagtaggc
     1801 tcgccaacct ttacgggctg gaaacgacgc tccaaagcgg catctttttc aatatatttt
     1861 ctgtactcgt taagagttgt tgcaccgata acctgaaaat cgcctcttgc aagtgacggt
     1921 ttaaggatat ttgcggcgtc tgcactgccc tctgcggcac ccgcaccaac gatagtatga
     1981 agttcatcaa tgaagaggat agtgttcttt gactttttaa cctcgtcaat agccgcctta
     2041 attctctcct caaagtcacc tctgtacttc gcacctgcta ccatacctgt gaggtcaagg
     2101 ctgacaactc gcttgtcttt aagaatttca ggcacattgc cctttgcaat ttcaagtgca
     2161 agtccctctg caaccgcagt tttacctaca ccgggttcac cgataagaac aggattgttc
     2221 tttgtacggc gggaaagaat ctggataacc ctctggattt ccttttctct gccgattaca
     2281 ggatcaattt caccgttctt tgcggcttga gtaagatctc tgccgaattt atcaagagcc
     2341 gaaccgcctt tctgtgaacc gttcggagcg gattcgtttt caaaaccgcc gaaaccgtca
     2401 tctgcaccgc cctgcatacc ttttgacaca gcctgtgcaa gtctttcaac agatacaccg
     2461 agttcttcaa gaaaagcaac tgcgtagctg tcgctctccg aaaggattgc aaggagcaaa
     2521 tgctctgtgc ctacataggt gtagcctgtt tttgaagaaa tgataactgc ggctctcaaa
     2581 actctctttg tacggggagt aaaatcatcg ggagtaagct caacgagcga cattgtaccg
     2641 ttaatggatt caagcttttc acggagagca tcttccgtta ctccgcactc attaagagcg
     2701 gttgcggcaa caccgctgcc ctcttttacg agtccgtaga gaatgtgttc tgtaccgaca
     2761 tagttgtggc gcatctcctc tgcactttca atcgcaagat taagcgcctt gtttgctttt
     2821 tctgtaaaac ctttgaactg atacataaaa tcatcaccct tccttgtgga taatgtatta
     2881 actgcttatt ttctatgaaa gagcctctct taaaattttt gccctttcaa tatctctttc
     2941 ctgtgcggaa agattttttc ctgagtttac cataagcgtt gcaggctcaa ttttttccat
     3001 aagagagtca acaacctctg cggtaacatc ttttatctgt cccgacaaaa tgccgaaacg
     3061 aacatttgac aacagcttta aagcctcgtc atgagtcata accattgccg atttcaacaa
     3121 gcctaagctt ctgcttacgc tgtcctgaat atcaatactc tttgcaagtc tttctcttgc
     3181 ctgccgttcc tgagaaacaa gctgttttgt gatgttctca agattttcaa tagcggcttt
     3241 ttcggagata ccgagcgtta cctgatttga aagctgataa agcgaaccgc tgggttctga
     3301 gccttcaccg taagcaccac ggattgtaag tccgagcttt gaaaggttgc ctgcaattct
     3361 gccgattgta cggctctttt caagtgccgg cagatggagc ataacagagg ctctcatgcc
     3421 tgtaccgagg tttgtagggc attgtgtaag ataaccgagc ttgtcatcaa aagcaaattc
     3481 aaggttttca tcaagcagag tatcaagctt atcggctgta tcatatgcct gttcaagcga
     3541 caagccgtct gtgataacct gaagtcttat gtgatcttcc tcgttaatca taatgctcat
     3601 gctttcatcc ttgctcaaca gcaatgctct gccctctgtt tcgctgatga attcgggact
     3661 tacaagatgt ctttcaacaa gtgacacacc ctgtgcctct gtcaaatcat caagcttaat
     3721 cagattgaaa tccgaggcaa ttgagctgtt gctgtccctg attgcacttg taaccttttc
     3781 aataaccttt tttctgccct gttcggacag tcggcacgga aacgggtaat ctttcaggtt
     3841 tcttgcaagt cttactctgg tggaaattac ttcgttactc ataattaagc ctccatttcc
     3901 ttaatctgat ctcttaactc tgcggcatgt tcaaaattct gttccgaaat tgccttgtcg
     3961 agttcctttt taagtgcctt gattttatct ttctttgtca tctcggcact cttttcggct
     4021 ttctttgtac cgaaacggct gttcttaccg caatgggttg tgttgccgtg gattcttctg
     4081 attgaaggaa gaagctggtc gccgaagatt tcgtaacatt ttgcacagcc tacatgaccc
     4141 gactttgcaa tatcggaata tgagcttccg cagaagtcac agcgtgttgc ctgagttctt
     4201 gcgggaaggc tgttgccgaa gaaacttccg aggagtcccg aaaattcgtc ctcaaagtta
     4261 ccgaagatgt ttgtgtatcc cattttcttt gcacattcac tgcacaaatc atattcttta
     4321 atttcaccgt tgataacggt ctttacatga gtatttgcat tgttagcacc gcatttttga
     4381 catttcataa taactgcctc cgttcaaaat caacttaatg tgagcaacat attcttgaac
     4441 aagtccgctc gcacggcatc tcgtttactc tgttcaacat ttgtaagcgt tctgtcggac
     4501 aacgccgagg ctatcagcct tgccgatgta agctcaatca tatctctttg caacatattt
     4561 ttgagcattg cctctgcact tgccttgtca agtgttgttc caatcgaatt tattatatgc
     4621 ataagcgccg tacctctatc catcttcaca cggcttattc ggataaatcc gccgccgccc
     4681 ctacggctct ctacaatgta gcctttttcg ggagtgaatc gggaggttat aacataattg
     4741 atttggctcg gtacacatcc gaggttgccg gcgagttcgt ttcgctgaat ctccgcactg
     4801 ccgttttcac ggtcaagcat ctctataatg tactgtgcca ccaaatcgct cattctcatt
     4861 ttggtcgcct cttttcattt gtttgactac attataatat caaggtcaga gaaagtcaaa
     4921 gtcaattaaa gtcaaaaatt aaagcataag ctttgaaatt ctcacgaatt ctcgcttatg
     4981 ctctcgtttc ctaatatttt aaaatatttt tgactttttc tgactttcga tttttatccg
     5041 ataaagtctg acttttcaag caatttgacc ttacataatt acattaaccg tgtaatcaac
     5101 atcgtaaatt tcaccgtcaa accaatttat aaaacctatg tagatgcaga ttttgtcatc
     5161 atgtttattc atatcacgaa caaccttaaa gcactccgac caacccgaaa agcgttcaag
     5221 tcttttgact gtatcaacat cggcattgcc gtttacaaac acacacattg caaggtgacg
     5281 ggttgacgat aacattctta caaaatcgag gaacatcata ttaacctcat cgcccgaaca
     5341 ttcaatttta agagcgtctt cctcgatttt gtttccgaaa aaatcaagca cattgtcaaa
     5401 cgctgaatca aagtcatcgg tttcagcgtt ctttagtccc tgcgaaaact tgtaaattgc
     5461 ggtagatgca taaattctct taattttttc aatcgtcacg gcaatcacct tctcggtatc
     5521 gttactttta ttattataca ttatataagc aaatttgaca acaagtatgt tattcgggcg
     5581 gttcaataca atttttgtaa taaaaacatt tgaaattaaa caaaaatttt gtgtttcccg
     5641 attgacaatt gtacagagat attgtattat ataattgtac taatgaatta gttcaattgc
     5701 ttgataagga gcgtgatttt atgtacagcg actttgcatt aagaacagtt ttatatacta
     5761 tactctgcat cggtgctatc ggaataagca caggaaaact gtttgccata aatcacggca
     5821 tttcaaaata tcttgctaaa aagtcatata gcggcaattt taaaacagta aaaacaacat
     5881 ttatttcact tggcgttgaa tatgtttcga tagcaataat tgcacttttc atcagtttat
     5941 gcaccataaa tacaatttac acaaatgcat tttttgatga actgttgcct gaatttttct
     6001 cgacagctgt taccgtattt gcaatcgttg ttcttggtgc agaatgtgat ttcggtttgt
     6061 ggttactctt tgcaataccc tatacttttc tgattattgc aaacattctc attctgtttt
     6121 tcatcaatta caaatttgtt tataaagatt ttgaagtccc taaaattaag aaaatgaaa
//
LOCUS       DAOMVE010000115         6121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3462_length_6121_cov_3.529344, whole genome shotgun sequence.
ACCESSION   DAOMVE010000115 DAOMVE010000000
VERSION     DAOMVE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3462_length_6121_cov_3.529344"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..399
                     /locus_tag="PLS64_07030"
     CDS             166..399
                     /locus_tag="PLS64_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43610.1"
                     /translation="MTESVLLIILLIALAEVINSIKNNRPTASNIGRLFYIRFKTLGL
                     TAFNGFPCVLIIYYAKSNVNIPFEFYIAENVER"
     gene            complement(424..1239)
                     /locus_tag="PLS64_07035"
     CDS             complement(424..1239)
                     /locus_tag="PLS64_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HRL43611.1"
                     /translation="MNFAEYNVKDANLEEVLLALKSLDTVEAVALGGSRSTGASDENS
                     DYDIYVYCAEIPTAETRENLLGKYCSIAEFGNHYWESEDNTVMNNGVPVDIIYREVDR
                     FGRYIDTVIKGGKAFNGYTTAFWHNIKNSKVLFDKTGTFTKFRDMAQIDFPENLRNAI
                     IKNNRNLLNGKLPSYDRQIKKAQERGDIVSVNHRITAFLESYFDVIFALNRQTHPGEK
                     RQVQLCKKYCTLLPKDFEENINALMKSISDGGSYKIVTAMVNNLDEILNDEFK"
     gene            complement(1285..2694)
                     /locus_tag="PLS64_07040"
     CDS             complement(1285..2694)
                     /locus_tag="PLS64_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL43612.1"
                     /translation="MSATSSKLKNPNDSVMNQRVIKLAWPIFLQLLLGISLGYVDTIM
                     LSNYNETAVGAIGNANQILGFLTLAFNIISSATGIIVSQYLGAGKKEKMNMIYTVALS
                     FNLVLSIVISFVVFIFSRNLLEAMQVPAAMLDESDMYMRIVGGTIFTQALINAFNSIF
                     ASNGKTVFGMIIGLGMNIINICGNALLLYGPLQVLGLGASGAAVSTCGSRVIAVIASV
                     IYFYTVIKGKFSLKYLRPFPYDVLKSLLKLGIPTAGENISYDCSQLFLQAFINTMGIV
                     AINAKIYANMLSMFTYMIALAAANATQIIVGHSVGAHDYDFAYRRVLKTLRFGMIISI
                     SVAIVNWLISPFTLGLFTGDKAVIALGSSVMFIAIILEFGRTTNLVIINSMKAAGDVK
                     FPTALAIFSMWLLSVGLGWLLGIYFGMGLTGIWIAMAADEIFRGVVVFIRWIKGGWRG
                     KRVVLEDKTSPTETAEVTA"
     gene            2943..3536
                     /locus_tag="PLS64_07045"
     CDS             2943..3536
                     /locus_tag="PLS64_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRL43613.1"
                     /translation="MNICVFGSSSEQIDKIYLESAEHLGKKIAEKGYNLVFGAGKYGV
                     MGASARGAYSAGGHTIGVTPDFFIDVGVVFDKCSELIVTDTMRERKGIMEDKADAFVI
                     CAGGMGTFEEFFEVLTLKQLNRHTKPIIIYNVKGYYNSMLAMLDNAVSEKFMTNACNR
                     LYTIADTEEEVFNQLENYVPFTYNKYEFLKKDGEQNG"
     gene            3529..3702
                     /locus_tag="PLS64_07050"
     CDS             3529..3702
                     /locus_tag="PLS64_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6472 family protein"
                     /protein_id="HRL43614.1"
                     /translation="MADQCEFCANFVSDDEFGDYCNINLDEDEMARFLTRSTDTCHYF
                     QLYDEYKIVRKQN"
     gene            4078..4896
                     /locus_tag="PLS64_07055"
     CDS             4078..4896
                     /locus_tag="PLS64_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43615.1"
                     /translation="MGLFNRIIDKMVSDKVPFKKRKRLIVFVAIITLLSMTTATVAWF
                     TVNTFAGVDKLDLNISLAAQLKVSMENHGKDLEKYGKVITNEMIDDYLKKHGSSLEDT
                     VLDPVTTREGTEFTNQRGTVREPNKHSYLEFECYFIATEEMWVHLTTESTKAGADDGT
                     KVSTESTGAKADVVNCARVDFTAEGAGNGTTIYEPNRSTPVNGQTTFDLPSGSMVYSN
                     NTRLFHLNQLEPVKVTVRLWIEGNDPECDDDVQDAQLSVQLGFVGCDKDNKPIS"
     gene            5048..5854
                     /locus_tag="PLS64_07060"
     CDS             5048..5854
                     /locus_tag="PLS64_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43616.1"
                     /translation="MFGKKKPKIEQPITPEELSRLNDKKMADAEIEHFHVKNDSIEIV
                     KYKKRRRLLSIILSVCIIILLILFIVSTLVTQWGDLIISIDSPAVKKGIVLSEDADFK
                     TQCASLTAEQVKDVTNITYSWLPVDLDTSKDGAHNGKNYVAYTFYCKNNGEVELDYDA
                     VLEITGAAKSADEATRVMIYKNGKSSIYGKGQYKDRSKAETDCTKFVSDKEVYKTSTE
                     KFKVGDIDKYTIVIWIEGNDPECIDDIRNGHVRMRMLFSVRDDEESTAKP"
BASE COUNT         1927 a         1195 c         1294 g         1705 t
ORIGIN      
        1 ataaccgaca atgcagttac ggcaaaagag attcttgatg agatgtatat gaagtaagcg
       61 tagtatttat ctatggttga catcgcaatt ccagcttgat acaatatgat acgagaatac
      121 aaggaagttt aaggcggtta gcactcccga aagggggtga tatgcatgac tgaatcggtt
      181 ttgctgataa ttttacttat agcactcgca gaagtcatca atagcataaa aaataatcgc
      241 ccgacagcct caaacatcgg acgattattt tatattcgtt ttaaaacttt gggactaacc
      301 gcttttaacg gttttccttg tgttcttatt atatattatg caaaaagcaa tgtcaatatt
      361 ccatttgaat tttatatagc tgaaaatgtt gagcggtgac ggaactttcc gacaccgctt
      421 tttttatttg aattcatcgt taaggatttc atcaaggtta ttgaccatcg ctgtgacgat
      481 tttgtatgaa ccgccgtctg agattgactt cataagagcg tttatgtttt cttcaaaatc
      541 ctttggcaag agggtgcaat actttttgca gagctgaacc tgtctttttt caccggggtg
      601 tgtctgacgg ttgagtgcga agataacatc aaaatagctt tcgagaaaag cggtgattct
      661 gtgattgaca ctgacgatat cgccacgctc ctgagccttt ttaatctgcc tgtcatatga
      721 cggaagcttt ccgttcaaaa ggttgcggtt gtttttgata atcgcattgc ggagattttc
      781 aggaaaatca atctgcgcca tatctctgaa ttttgtgaaa gtgcctgtct tgtcaaaaag
      841 aaccttgctg ttcttgatat tgtgccaaaa tgcggttgta tagccgttaa aagccttgcc
      901 gcccttgatt accgtatcaa tataccgacc gaacctgtca acttcacggt agattatgtc
      961 aacgggaaca ccgttattca taaccgtgtt gtcctcgctc tcccaataat gattgccaaa
     1021 ttctgcaatc gaacagtatt ttccgaggag attttctctt gtttcggctg tcggaatttc
     1081 agcgcagtaa acataaatat cgtagtccga attttcatca cttgcacctg ttgaacgaga
     1141 accgccgagt gcgacagcct caaccgtatc aagcgacttg agtgcaagca aaacttcttc
     1201 aaggtttgcg tccttaacat tgtattccgc aaaattcata gttttgatct cctttatttt
     1261 atggtatcat attcctttgt cggattaagc cgtaacttct gccgtttcgg ttggacttgt
     1321 cttatcttca agcacaaccc ttttgcctct ccaaccgccc tttatccagc ggataaacac
     1381 aacgacgcct ctgaaaattt catcggcagc catggcaatc caaattccgg taagtcccat
     1441 tccgaaataa attccgagca gccagccgag tccgacactc aaaagccaca ttgaaaaaat
     1501 tgcgagtgca gtcgggaatt taacatcgcc ggcggctttc atactgttga ttataacaag
     1561 gtttgttgtt ctgccaaatt caagaataat cgcaatgaac ataaccgatg aaccgagtgc
     1621 gataacagcc ttatcacccg tgaaaagtcc gagtgtaaac ggcgaaatca accagtttac
     1681 aattgcaacc gagattgaaa ttatcatacc gaaacgcaaa gttttcagca ctctgcggta
     1741 tgcaaaatcg taatcgtgag cgcctacgct gtgaccgaca ataatctgtg ttgcatttgc
     1801 agcggcaaga gcaatcatat atgtgaacat tgaaagcata ttggcgtaga tttttgcgtt
     1861 gattgcaacg atacccattg tgttgatgaa tgcctgtaaa aacagctgtg aacagtcgta
     1921 ggaaatgttc tcgcctgctg tcggaatgcc gagtttaaga aggcttttga gaacatcata
     1981 cggaaacggg cgaagatatt taaggctgaa cttgcctttg ataaccgtgt agaagtatat
     2041 aacggatgca attaccgcaa taactctgct tccgcatgtg gaaacagccg cacccgacgc
     2101 accgagtccg agaacctgaa gcggtccgta gagcaaaaga gcatttccgc agatattgat
     2161 aatgttcatt ccaagaccga taatcatacc gaaaacggtt ttgccgttgc ttgcaaaaat
     2221 tgagttaaaa gcattaataa gcgcctgagt aaagattgta ccgccgacta ttcgcatata
     2281 catatccgat tcatcaagca ttgccgcagg cacctgcatt gcctcaagca gattccgact
     2341 gaatatgaat acaacaaagc tgattacaat actcagcaca aggttgaatg acaacgcaac
     2401 cgtgtaaatc atattcatct tttctttttt acctgcccca aggtactggc tgacgattat
     2461 gcccgttgcg cttgatataa tgttaaaggc aagagtcaga aaaccgagaa tctggtttgc
     2521 gttgccgatt gcaccaacgg cggtttcgtt gtagtttgag agcataatcg tgtcaacata
     2581 gccgagtgag atgccgagca acagctgtaa aaatatcggc cacgcaagct tgattacgcg
     2641 ctgattcata actgaatcgt tcggattttt gagttttgaa gaagtagcag acataatgta
     2701 atctccgttg aataaaatga ttaatataag tttttatgtt ttgaatgttt tgaaaaaata
     2761 aattctgtgt aaattgtgca ttgccgttgt tctgactgct tgaccgccgt gtatttgaga
     2821 tgtataatct aattgcggat ggttgcaatc tgtcgcaatc ttcaaactaa gttcagataa
     2881 ccgacggttg ttttgaataa aatcagaaaa aatacaacgc aaatgcgaaa ggataaatag
     2941 gtatgaatat atgcgttttt ggctcatcaa gtgagcaaat tgacaaaatc tatctcgaat
     3001 ctgccgagca tctcggcaaa aagattgccg aaaagggcta caaccttgtt ttcggtgcgg
     3061 gaaaatacgg tgttatgggc gcatctgccc gaggcgcata cagcgcaggt ggccacacaa
     3121 tcggagttac acccgatttt tttattgatg tgggtgttgt ttttgacaaa tgctccgaac
     3181 ttattgtaac agatacaatg cgtgaacgca aaggtattat ggaagataaa gcggacgctt
     3241 ttgtaatctg tgccggcggt atgggtactt ttgaagaatt ttttgaggtt ctcacactaa
     3301 agcagcttaa cagacacaca aagcctatta ttatatataa tgtaaaaggc tactataact
     3361 caatgcttgc aatgcttgac aatgccgtaa gtgaaaagtt tatgaccaat gcctgtaaca
     3421 gactctacac gattgccgat accgaagaag aggttttcaa ccagctcgag aattatgttc
     3481 cgttcaccta caataaatat gagtttctca aaaaggacgg tgagcagaat ggctgaccaa
     3541 tgcgaatttt gtgcaaactt tgtcagtgat gacgaattcg gtgattattg caatatcaac
     3601 cttgacgagg acgaaatggc aaggttttta acccgaagca cagacacctg tcattacttt
     3661 caactttatg atgagtacaa aatagtgaga aaacaaaatt aaataattta aaacagcaat
     3721 ataacaaaat aagttacacg gttgtaactt ttgcctcaaa tttaattaaa tgtcgtttgt
     3781 tattattgta aacctaaaat ccccttaatt aaaggggatt ttttgttttt ttatatcatt
     3841 ttaaaggtta gaatatgaat aaaagacaag cagattaagt ccgttaaaat ataatcgaaa
     3901 aagaaaagtt tagaggaatg cacaaatgta atgtgaaaat cttgtgaatt catccaaaaa
     3961 aaatgacaga ggacaaataa atattgcaaa atgtgcgttg aaggtataga atattattat
     4021 attttgtaca gtggtgtgcc attttagtgc attatttttt aagaggagag ggtttttgtg
     4081 ggccttttta acaggattat tgataagatg gtcagcgaca aagttccatt taaaaagaga
     4141 aaaaggctga ttgtttttgt tgcaatcata acccttcttt caatgacaac agcaacggtt
     4201 gcgtggttca cggtaaatac atttgcaggt gtggacaagc ttgacctcaa tatcagtctt
     4261 gccgctcagc ttaaagtaag tatggaaaac cacggcaaag accttgaaaa atacggcaag
     4321 gtaatcacaa atgaaatgat tgacgattac cttaaaaagc acggttcaag ccttgaagat
     4381 acggtgcttg accctgttac aacaagagag ggtacagagt tcaccaatca gcgcggcaca
     4441 gtcagagagc ctaacaagca cagctacctt gagtttgaat gctacttcat cgcaaccgag
     4501 gaaatgtggg ttcacctcac aaccgaaagc acaaaagcag gcgctgatga cggtacaaag
     4561 gtatcgacag aatcaaccgg tgcaaaggct gatgtagtaa attgtgcgag agttgatttc
     4621 acagccgagg gagcaggcaa cggcacaaca atttatgagc cgaacagaag tactcccgta
     4681 aacggacaga caacttttga tttgccgtca ggttcaatgg tttactcaaa caacacaaga
     4741 cttttccatc ttaatcagct cgaacctgtt aaggtaacag ttcgcctttg gattgagggt
     4801 aacgaccccg agtgtgatga cgatgttcag gatgcacagc tttctgtaca gcttggcttt
     4861 gtcggatgcg ataaggacaa taaaccaata tcataattaa acaagatccc gtaggggcgg
     4921 tcatcggccg cccgccctgg agaagctgtc attcaacgca gatttcagac aggagaaaaa
     4981 ttatgggtta atgaaatacg cacaatccga cagttaaacg gatacaactt tgaggggaga
     5041 gaaaaacttg ttcggaaaaa agaaaccaaa aattgaacaa ccgattactc ccgaggagtt
     5101 aagtcgctta aacgataaga aaatggcaga tgccgagatt gaacattttc atgtaaaaaa
     5161 tgattcaatc gagattgtaa aatacaaaaa gcgcagacgg cttttgagta ttatcctttc
     5221 ggtctgcata atcattcttc ttattctgtt cattgtttca acgcttgtaa cgcaatgggg
     5281 cgacttgatt atcagcattg attctcctgc cgtaaaaaaa ggtatcgtgc ttagcgaaga
     5341 cgctgacttc aaaactcagt gtgcttcact tacagcggag caggttaagg atgttacaaa
     5401 cattacatat tcatggcttc ctgttgacct tgacacaagc aaggacggtg cgcacaacgg
     5461 caaaaactat gttgcttata ctttctattg taaaaataac ggagaggtcg agcttgacta
     5521 tgatgcagtg cttgaaatca caggtgcggc taagtcggca gatgaagcaa cccgagttat
     5581 gatttacaaa aacggcaaga gctcaattta cggcaaaggt cagtacaaag acagaagcaa
     5641 agccgaaacc gattgcacaa agtttgtaag cgataaggaa gtttataaaa catcaaccga
     5701 aaaattcaag gttggcgata ttgacaaata cacaatcgta atctggattg agggtaacga
     5761 ccccgaatgt atcgacgata tccgtaacgg tcatgtaaga atgagaatgc tgttctcggt
     5821 tcgtgacgat gaagaatcca cagcaaaacc gtaattttcg gtacagtatt acatcaactt
     5881 ggtattatgt gcctcggcac tgatatagca aaaaatccct atggtgtttt agggacaaaa
     5941 aattatttta tttaggagga atttctctat gaaaacaaca tcaagaaaga gattattaat
     6001 ctcttccgta gcaatgctcc tcgtggcaat gctcgcactt ggtacagcaa catttgcttg
     6061 gttcacaaca agcacaaatc cttatgctga taactttagt gcaaaaacta caaagcaaag
     6121 t
//
LOCUS       DAOMVE010000116         6072 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3493_length_6072_cov_2.864384, whole genome shotgun sequence.
ACCESSION   DAOMVE010000116 DAOMVE010000000
VERSION     DAOMVE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3493_length_6072_cov_2.864384"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2110)
                     /locus_tag="PLS64_07065"
     CDS             complement(<1..2110)
                     /locus_tag="PLS64_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRL43617.1"
                     /translation="MSRNWTPQQKNAIYATDGSVLVSAAAGSGKTAVLVERVIKLITR
                     EKNPLDVDRLLIVTFTRAAAAEMRERIQAAVNNLLEDDPYNAHLLAQRQLLYSANIST
                     IDSFCGDIVREYFHTLDVARDFRIADEGELEILRQEALDNTFESFYDSEDDCFYSLLD
                     LFKGKQGDQKLRETVLKISEFLTTQPFPDKWLDDMLENYSETDIEKTVWGKIIIDYAL
                     SAVLHGINLTENSILRLKDDEKLQDAVEPLLVGDLAYFTLVQLKFVSSSWDEIRQAIF
                     SFETGRMKSPKGYKDNPIKISVSSNRDEVKETVKKLQSLFFWSEEDARLELSQLHRFI
                     STLFTLVRAYDKKLSELKAKKNILSFADIESLTVKLLAKPDDDNGYTKTVQAQEISNR
                     FDAVMVDEFQDVNDVQDLIFKSVSNDESNLFVVGDVKQSIYGFRQAKPEIFIERKNEY
                     KRFNEENPEYPATIILDRNFRSRFEVCDAVNFIFERIMTKESAKMEYNSDERLVNGAE
                     FPKSDDCNFEISLIESENSDLEKEEIEAKYIADKIHDMINSGFRVKDGDIMREARYGD
                     FAIILRSPSGKAATYVNTLNNSGIPAYSENKSSFFDAVEIKIMLNLLRIIDNPGIDIP
                     LLSVLCSPMYAFTPDELAEMRCESRKSSLYSSVCEYAKTNDKARKFVDELKILRDCAC
                     TNSVDALISKACEMTGFMSIS"
     gene            complement(2110..5448)
                     /locus_tag="PLS64_07070"
     CDS             complement(2110..5448)
                     /locus_tag="PLS64_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRL43618.1"
                     /translation="MLRFILGKSGSGKSTETLKIAGELADSNLPVMLIVPDQSTFECE
                     KTLLDVFGAKKAEKVLVFGFSRLCKYVFELTGNSADNVIDEGTRAVVMSLALEELTEK
                     LRLLSSKGNKSLVDVMLQTVSDLKKSSVTSDELRSASMLVDDETLKTKLNETALISDT
                     FDALVSQSYIDPMDDLTRLNDILLNNNVLRGYTIIFDGFSGFTMQQLKVVRSLIRDCT
                     ATYFTLTLDPLTDGSEEVFATSQQTYKILKEYAKRDGVDIKAPIKLTDLHRFKNDNLA
                     LLEQNIFRPHIEPLEIVPENICVYNAVDMYDECEYVAGKIKHLVIEKGYLYSDISVIC
                     HDTEPYRGVLSEILEKYEIPYFSDEHADIDVKPVIRLVNSIFRCLIYDFDREDVLALL
                     KSGLTEFSVEEISLFENYLFVWNVSGSAFKNKFVQNPKGYSDRFSDYDREVLAKVENL
                     RESVIEPLLLFKENAKDKNGLEISKLFYALLEELKVPDALRRMYAEFERSYDKSVGAE
                     QIRVWNMFMAVLDKTAAVIGTKTMTVKRYYELLSLQINAMQLSDIPRTIDCVTITTAQ
                     RVRISKQKASFLIGCVDGIFPAIPHTSGIFSPFELKMLAMNEIELGDDFAAHNNLETF
                     MAYSCMTSASEFLSVSFYSSDITGTTYQPSEIVLQCKKVFKNIIAYDKFDFDGKKESM
                     YALRPAFDTVADMIGKGKKVPESLLEIFAQNPEYSAKLDSLINAKDKVPFKIFDKKNA
                     EKLFGKNLNISASQLEKFNLCRFSYFCNYGLNVRERQRAEINPMQYGTIVHYILERFF
                     GEYTKEEYLSLEEDKFQKIVCAYLAEYAVKYFGEEQLESNSFTYRLKTILDNVMYLMK
                     HIMEELSQSEFFVADCELKIGEDIPSYTIVLPDGHKIAVCGSVDRVDIMQRADITYLR
                     IIDYKTGTKQFKLSDILYGINLQMLLYLHSIEASGGDKYGEIVPAGILYMPATVPYIS
                     SDSLKSIDKLPDELNKKLKMNGLLLKDTEIIHGMDKTDVATYIPVKIKAGEPVSSTSL
                     ATLAEFGKIFKKVDMLIAEMGKNIYNGNIEAKPLKGGHDSCEYCPYDSVCAYRQSNPI
                     TCFSVDNKTVIEEITNEINGKEEE"
     gene            complement(5552..5701)
                     /locus_tag="PLS64_07075"
     CDS             complement(5552..5701)
                     /locus_tag="PLS64_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43619.1"
                     /translation="MYPSICISTADEGKDRAKDVLSDDEYSVVTDDKVEYKFFIVELF
                     QKIFV"
     gene            complement(5701..>6072)
                     /locus_tag="PLS64_07080"
     CDS             complement(5701..>6072)
                     /locus_tag="PLS64_07080"
                     /inference="COORDINATES: protein motif:HMM:NF021093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HRL43620.1"
                     /translation="LYSVIPFQAVCAEIPNNVFRFHILANSDTEEDQTLKLKVRDKVL
                     ERTKILFDTANSKSDAEEFVKANLETIEEIAQNEVYKNGYNYPVKAEIVNMHFDTRHY
                     ESYTLPAGMYDAMQKGIIGGA"
BASE COUNT         1765 a         1250 c         1120 g         1937 t
ORIGIN      
        1 gggaaataga cataaatcct gtcatttcac acgcttttga aatgagagcg tcaacggaat
       61 ttgtacaggc acaatcacgc agaattttaa gttcatcaac aaattttctt gccttatcgt
      121 ttgtttttgc atattcgcag acagaagagt aaagcgaaga ttttctgctt tcacaacgca
      181 tttcggcaag ctcatcggga gtgaacgcat acataggact gcacaaaacc gacaaaagcg
      241 gaatatcaat ccccgggttg tcaattatac gcagaagatt gagcataatt ttgatttcaa
      301 ctgcatcaaa aaagcttgac ttattctcgc tgtaggcagg aataccgctg ttgttcagag
      361 tgttgacata agttgcggcc tttccgcttg gactgcgaag aattattgca aagtcgccgt
      421 aacgggcttc acgcattata tctccgtcct taactctaaa gcccgaatta atcatatcgt
      481 gaattttatc cgctatgtac tttgcctcaa tttcttcttt ttcaaggtct gagttttcgg
      541 attcaatcaa tgaaatttca aaattgcaat catcagactt tggaaactca gcaccgttta
      601 ccagcctttc atcggaatta tattccattt ttgcggattc cttggtcatt attctttcaa
      661 aaataaaatt gaccgcatca cacacctcaa aacgactgcg gaaatttctg tcaagaatta
      721 tagttgcagg atattcggga ttttcttcat tgaatcgttt atattcattt ttgcgttcaa
      781 taaaaatttc gggctttgcc tgacgaaatc cgtagatact ctgctttaca tcgccaacca
      841 caaaaagatt gctttcatca ttgcttaccg acttgaagat aaggtcttga acatcattta
      901 catcttgaaa ttcgtcaacc ataacagcat caaatctgtt tgaaatctcc tgagcctgaa
      961 cggttttggt atagccgtta tcatcatcgg gctttgcaag aagttttaca gtaagcgatt
     1021 caatatcggc aaacgagaga atatttttct tcgctttgag ttcggaaagc tttttgtcat
     1081 atgcacgaac aagtgtaaac aaggtagata tgaacctatg aagctgtgac aattcaagcc
     1141 ttgcatcttc ctcactccaa aaaaacaatg attggagttt cttcaccgtt tctttgacct
     1201 catctctgtt agatgaaaca gaaattttaa tcggattatc cttgtatcct ttcggacttt
     1261 tcattcttcc tgtttcaaaa gaaaatattg cctgtctgat ttcatcccat gatgaagaaa
     1321 caaatttcaa ttgaacaagg gtaaaatatg ccaaatcacc gacaagaagg ggttcaactg
     1381 catcctgaag tttttcgtca tcttttaatc gaagaataga attttcagtc agattgattc
     1441 cgtgaagcac ggcactcaga gcatagtcga ttataatttt gccccacaca gttttttcaa
     1501 tatcggtttc agaatagttt tcgagcatat catcaagcca tttatcgggg aacggctgag
     1561 ttgtaagaaa ctcggatatt ttaaggactg tttcacgcag tttttggtcg ccttgtttcc
     1621 ctttaaaaag atcaagcaaa gaataaaagc agtcatcttc gctgtcataa aagctttcaa
     1681 atgtattatc aagtgcctct tgtctgagaa tttcaagctc accctcatcg gcaatacgaa
     1741 aatctcttgc aacatcgagc gtatgaaaat attcacggac aatatctccg caaaagctgt
     1801 ctattgtaga aatatttgca ctgtacaaaa gctgtcgctg tgcaagcaaa tgagcgttat
     1861 acggatcatc ttcaagaagg ttgttgactg ccgcctgtat tctttcacgc atttcggcag
     1921 ccgccgctct tgtgaatgtt acgataagaa ggcggtcaac atcaagggga tttttctccc
     1981 gtgtaataag ctttattacc cgctctacaa gcacggcagt tttgcccgaa ccggcggcag
     2041 ccgatacaag cacagaaccg tctgttgcat agattgcatt cttctgttgc ggagtccaat
     2101 ttcttgacat tactcttcct cctttccgtt aatttcgttt gtaatttctt cgataacggt
     2161 cttgttatcg acagaaaaac aagttatcgg gttactctgt ctgtaggcac atacgctgtc
     2221 gtaagggcag tattcgcacg aatcgtgacc gcctttgagc ggtttcgcct caatgttgcc
     2281 gttatagata tttttgccca tttcagcgat gagcatatca acctttttaa agattttacc
     2341 gaattctgca agtgttgcaa ggcttgttga agaaacaggt tcgcccgctt taatttttac
     2401 aggaatgtat gttgcgacgt ctgtcttatc cattccgtga attatttctg tatctttgag
     2461 caaaagtccg ttcatcttga gctttttatt aagttcatcg ggcagcttgt caattgattt
     2521 cagactgtct gacgaaatat acggaacggt tgcaggcata tacaaaatcc cggcaggtac
     2581 aatttctccg tatttgtcac cgcccgaagc ctcgattgaa tggagataca aaagcatctg
     2641 aagattgatt ccgtagagaa tatcggaaag cttaaactgt tttgtgcctg ttttataatc
     2701 aataatacgc aaatatgtta tatcggctct ctgcataata tctactctgt caacacttcc
     2761 gcagactgca attttgtgac cgtcgggcag tacgattgta tatgacggaa tatcctcgcc
     2821 aattttaagt tcacaatcgg caacaaaaaa ttcactctgg gaaagctcct ccataatatg
     2881 tttcatcaga tacatcacat tatcaagaat tgtctttagg cggtatgtga atgaatttga
     2941 ctcaagctgt tcttcaccga aatatttaac tgcatattcc gcaagatatg cacagacaat
     3001 tttttggaat ttatcttctt caagcgataa atactcttcc ttagtatatt cgccgaaaaa
     3061 gcgttcaagt atataatgaa caatcgttcc gtactgcatg gggttgattt ctgctctttg
     3121 tctttcccga acattcaagc cgtaattaca aaaataggaa aatctgcaga gattgaattt
     3181 ttcaagctgt gaagctgaaa tattgagatt tttaccaaaa agcttttcgg catttttctt
     3241 gtcaaatatc ttaaagggaa ctttgtcctt tgcatttatg agcgaatcaa gctttgcaga
     3301 atattcggga ttttgtgcaa aaatttcaag caatgattcg ggcacttttt tgccttttcc
     3361 gatcatatcg gcaactgtat caaaagcagg gcggagtgcg tacatacttt cttttttacc
     3421 gtcaaaatca aatttgtcat atgcaatgat atttttaaat acctttttac attgcaaaac
     3481 aatttcggac ggctgatagg ttgttcccgt tatgtccgat gaataaaatg atacggaaag
     3541 aaactcactt gcagaggtca tacaggaata tgccataaat gtttcaaggt tattgtgtgc
     3601 ggcaaaatcg tcaccgagtt caatttcgtt cattgcaagc atctttaact caaacggtga
     3661 gaatatgcct gatgtatgcg gtattgccgg gaaaattccg tcaacacagc caatgagaaa
     3721 tgacgctttc tgcttagaaa ttcgtaccct ttgagcggtt gttatcgtta cgcaatctat
     3781 tgttctcggg atatcggaca attgcattgc gttaatctgt aagctcaaaa gctcgtaata
     3841 acgctttaca gtcattgttt ttgtaccgat tacagcggcg gttttgtcaa gaacagccat
     3901 aaacatattc caaactctta tctgctccgc acccaccgat ttatcgtatg agcgttcaaa
     3961 ttccgcatac attctgcgta acgcatcggg aactttaagt tcttcaagaa gggcatagaa
     4021 cagctttgaa atttcaagtc cgtttttatc ctttgcattt tctttgaaaa ggagtaacgg
     4081 ttcaataact gattcacgca gattttcaac ctttgcaaga acctctctgt cataatccga
     4141 aaacctgtca gaatagcctt ttggattctg aacaaattta tttttaaaag ccgagcctga
     4201 aacattccat acaaagaggt agttttcaaa aagacttatt tcttcaacag aaaattcagt
     4261 taaacccgat ttcaaaagtg caagcacatc ttctctgtca aaatcgtaaa taaggcatct
     4321 gaaaattgag tttacaagcc ttattaccgg ttttacatca atatcggcgt gttcatcgct
     4381 gaaatacggg atttcgtatt tttcaagaat ttcggacaaa acacctctgt acggttctgt
     4441 atcgtgacaa attacggaaa tgtcggagta gagataacct ttttctataa caaggtgctt
     4501 gattttaccg gcaacatatt cgcactcgtc atacatatca acggcattat aaacacagat
     4561 attttcagga acaatttcaa gcggttcaat gtgcggtctg aaaatattct gttcaagcaa
     4621 agcaaggtta tcatttttaa agcggtgcaa atcggtaagc tttatcggag ctttaatatc
     4681 aactccgtca cgctttgcat attccttgag aattttatat gtctgttgtg aggttgcaaa
     4741 aacctcctca gaaccgtctg taaggggatc aagagtaagg gtaaaatatg tagcggtaca
     4801 gtcacgaata aggcttcgta ccaccttgag ctgttgcatt gtaaagcctg aaaatccgtc
     4861 aaagattatc gtataacccc taagaacatt attatttaaa agaatatcat taagccttgt
     4921 caagtcatcc ataggatcaa tatagctttg gctgacaaga gcgtcgaatg tatcgcttat
     4981 gagggcggtt tcgttgagtt ttgtctttaa ggtttcatcg tcaacaagca ttgacgcact
     5041 tctgagttca tcgcttgtaa ccgaactttt cttcaaatcg gaaacagtct gcagcataac
     5101 atcaacaagg cttttgttgc cctttgatga aagcagacgg agtttttcgg taagttcttc
     5161 aagcgcaagg ctcattacga cggctcttgt accctcgtca ataacattat cggctgaatt
     5221 tcccgtcaac tcaaaaacat atttgcaaag tcttgaaaaa ccgaatacaa gaactttctc
     5281 ggcttttttt gcaccgaaca catcgagcaa ggttttttca cattcaaatg tgctctggtc
     5341 aggcacaatc agcattacgg gaagattgct gtcggcaagc tcgcctgcaa tcttcaaagt
     5401 ctcggtactt tttccgctgc cgctttttcc gagaataaat ctcaacattt catcacttcc
     5461 ataaatttaa tttacaacaa agtaatttta ttttaaatat ctgtacaaat aataacatat
     5521 tttgtgggtt atttaaagaa ctttgcagtt gttaaacaaa aattttctga aaaagttcta
     5581 ctatgaaaaa tttatattcc accttatcat cagttactac gctgtattcg tcatccgaaa
     5641 gaacatcttt tgccctgtct tttccctcat ctgccgttga aatgcagatt gacgggtaca
     5701 ttacgcacca ccaattatgc ccttttgcat tgcgtcgtac attccggcag gcagagtgta
     5761 gctttcataa tggcgggtat caaagtgcat attgacaatt tcagctttta caggatagtt
     5821 atagccgttt ttgtacacct cattttgtgc aatttcttct atagtttcaa ggtttgcttt
     5881 tacaaattct tcggcatccg actttgaatt tgccgtatca aaaagaattt ttgtcctttc
     5941 aagaaccttg tcacgcacct tgagcttcag agtctgatct tcttccgtgt cagaatttgc
     6001 aagtatgtga aaacgaaaaa cattgttagg gatttcagca cacaccgcct gaaaaggaat
     6061 taccgaatag ag
//
LOCUS       DAOMVE010000117         6026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3532_length_6026_cov_2.876570, whole genome shotgun sequence.
ACCESSION   DAOMVE010000117 DAOMVE010000000
VERSION     DAOMVE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3532_length_6026_cov_2.876570"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PLS64_07085"
     CDS             <1..926
                     /locus_tag="PLS64_07085"
                     /inference="COORDINATES: protein motif:HMM:NF040318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HRL43621.1"
                     /translation="FTAALNCLCKNISRRLLPLAPKLVDSVQEIRLRLSRPLALVCPD
                     NTYYLTQNGGLSNTILDGAMLVVSKADIVDTFNNICNYSVYNRQNEIVNGFVTMYGGH
                     RAGICGTAVVNNGKIVNIRDITSINVRIAREHKGCADSLYNKIIAVSGGVLICGAPCS
                     GKTTVLRDLARLLSTKGKKNVALIDERGELAGTASGKFQNDIGMCDVYDSYNKSEAML
                     HAIRSMAPEIVICDEIGSKEDIYAVEKSINCGVRIISSTHCANEYELKQKANMVSLMK
                     TGAFQTLVFLSNRTHAGEIVKISQVGDNFAV"
     assembly_gap    1061..1070
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1071..1421
                     /locus_tag="PLS64_07090"
     CDS             <1071..1421
                     /locus_tag="PLS64_07090"
                     /inference="COORDINATES: protein motif:HMM:NF021090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HRL43622.1"
                     /translation="YSGDDIFTLVNFCAENSSLDLLLFSECDRPFDELWLERVKQLSS
                     EIPLSKSDISMLNDFGGQLGKTDTEGQLKHLELYEVSFSKQLSSALDAITKKSKLYKT
                     MGFFAGSAIALMMI"
     gene            1430..1624
                     /gene="spoIIIAC"
                     /locus_tag="PLS64_07095"
     CDS             1430..1624
                     /gene="spoIIIAC"
                     /locus_tag="PLS64_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HRL43623.1"
                     /translation="MDVELIFKIAAVGIIVAVLTQLLIRSGREEQAMLTSLAGLIVVL
                     TLIITQISTLFNTIKQLFGF"
     gene            1626..2015
                     /locus_tag="PLS64_07100"
     CDS             1626..2015
                     /locus_tag="PLS64_07100"
                     /inference="COORDINATES: protein motif:HMM:NF018401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HRL43624.1"
                     /translation="MNILSISVLAVVTVIICVMLKPKNAEISVMLGISCSVIILIGVL
                     TQVSAIVTTINQIIASSGISIDYIVILLKSIGICLVTEFAVNTCKDSGSQSLASNVSL
                     AGKIMVTVTALPLYSDILKTVLSLAGN"
     gene            2025..3143
                     /locus_tag="PLS64_07105"
     CDS             2025..3143
                     /locus_tag="PLS64_07105"
                     /inference="COORDINATES: protein motif:HMM:NF021088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43625.1"
                     /translation="MKKSVVIITVVFVLIALSCVCANAEEGEMIYDMSNVFDSLSDDI
                     KQSLQNMGVDLGDFSKLANLSFENVMGEILSLALGNISSPLKGLVNITALLLLCSVIS
                     AYNGSLSCDISNSLNITATLCITCAAVVPAVGVIASTGSVICTASNIMLAYVPVMAVI
                     MASSGSVAGSASYYAVMIGAGEGVSQLSSKIIVPLLNMFLGLSITSSVSPSVNLSGFM
                     SMISKSVKWLLGFAMTIFTAVLTFRQLISVSVDNVSTRAVRFTLNSFIPIVGSALSDA
                     YKVVQGSIGLLKSGIGIIVILSVTIVFMPVILQGVMWMFTLWIGKSTAEVLNLNGPAK
                     LLENISSVFSTLIAVLLCIMSIYILSTAAVIMLGGGSS"
     gene            3140..3613
                     /locus_tag="PLS64_07110"
     CDS             3140..3613
                     /locus_tag="PLS64_07110"
                     /inference="COORDINATES: protein motif:HMM:NF021121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HRL43626.1"
                     /translation="MSGLFTVVVVMCASALICSLVQTFVTDGSTKKIISLVLGAFIIC
                     CMVVPVKNAVSSFNTDIEEKQNSVKEISTDDEAYSNAVIKQTEINLETALKNLLLQNN
                     ITIKSCDIILSRVENNGIIIASISIYIDKEFVRYTNLVSSIVYDNFGITPNIMTE"
     gene            3617..4066
                     /locus_tag="PLS64_07115"
     CDS             3617..4066
                     /locus_tag="PLS64_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43627.1"
                     /translation="MEDKTSSKLKSMLDSKKIRNIIIVVGLIGIGLIFLSNWVDFGSL
                     TSGKGDEAFSVKTYSTKIENDLQTVISKIEGAGKTEVLLTMENSVEYVYLDNSTTKTK
                     EIEPLIRGVLVVCEGGDDPVVVERVTDAVTKALDISTAKVCITKLSE"
     gene            4081..4623
                     /locus_tag="PLS64_07120"
     CDS             4081..4623
                     /locus_tag="PLS64_07120"
                     /inference="COORDINATES: protein motif:HMM:NF024097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HRL43628.1"
                     /translation="MKSKKKYIVFASLVLALSAAVYINWQLSGAKTPQSAKELGAASY
                     VSATASASDDEVQQTAALSNESKSYFSAERTKRQATQDKIIDDAKEIFNLESSSDADK
                     SEAEKNVEELLKTFTIQDSIESIVKAKGFSECLCYISDQGVSVIVPKSQLDDTSVLII
                     DDAVVTHYEVDYDDISVIGA"
     gene            4857..5351
                     /gene="nusB"
                     /locus_tag="PLS64_07125"
     CDS             4857..5351
                     /gene="nusB"
                     /locus_tag="PLS64_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRL43629.1"
                     /translation="MNNEINNELNEIEVNDAETSDIFEEEVNLTRSESREQAFMLLFS
                     KSFDDEPLEETIDDNSEMFVGGVCAYAQAVVSGIEDKHEEIDEIIASHLKKGWTLSRI
                     SKPSLAILRLAIYEMKYLDNVPQSVSINEAVELAKKYTIDESKFVNGILGTYSREFSG
                     KSEK"
     gene            5353..>6026
                     /locus_tag="PLS64_07130"
     CDS             5353..>6026
                     /locus_tag="PLS64_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M22"
                     /protein_id="HRL43630.1"
                     /translation="MSLFLGIDTSNYTTSTALYDSKTGEMVQQKKLLPVKEGQLGLRQ
                     SDAVFHHTAQLHTLIEELLKDVDTSKIAAIAASSRPRPVDGSYMPCFTVGVNTAKNLS
                     SAMKIPLYTFSHQEGHISAALFGSQRTDLFDKQFIAFHVSGGTTEAVFAKGFGTGFSV
                     ELAAHTLDLNAGQAIDRTGLMLGLKFPCGPQLEQLALKNTEKIKVKPTLKGCDCCLSG
                     VENLSK"
BASE COUNT         1788 a         1165 c         1328 g         1735 t
ORIGIN      
        1 gatttacggc ggcactcaac tgcctttgca agaatatttc acgcagactt ttaccgcttg
       61 cgcctaaatt agtcgattcg gttcaggaaa tcagactgcg gctttcacga ccgcttgcac
      121 ttgtctgtcc cgacaataca tactacctaa cgcaaaacgg cggacttagc aatacaatcc
      181 ttgacggtgc aatgcttgtt gtgtcaaaag ccgatattgt cgatactttc aacaacattt
      241 gcaactactc cgtgtataac agacagaacg aaattgtaaa cggctttgtc acgatgtacg
      301 gcggtcacag ggcaggaatc tgcggaacag cggtcgtaaa caacggaaaa attgtgaata
      361 tccgtgacat aacctcaatc aatgtccgca tagcaaggga gcataagggt tgtgcggatt
      421 ctctttataa taagataatt gccgtttcgg gaggtgttct catatgcgga gcaccttgca
      481 gcggaaaaac aaccgtactt cgtgaccttg caagacttct gtcaaccaag ggcaagaaga
      541 atgttgccct gattgatgaa agaggagagc ttgcaggcac ggcatcgggt aaatttcaga
      601 atgatatcgg aatgtgcgat gtctatgatt catacaacaa gagcgaggca atgcttcacg
      661 caatccgcag tatggcaccc gaaattgtca tctgcgatga aatcggctca aaagaagata
      721 tctatgccgt agaaaagtcg atcaactgcg gagttcggat aatttcatca acgcattgtg
      781 caaatgaata cgaactgaaa cagaaagcaa acatggtttc tctcatgaaa acaggggcat
      841 ttcaaacgct tgtgtttctg tcaaatcgaa cccacgcagg tgaaattgtg aaaatatcgc
      901 aggtaggtga caactttgcc gtttaaactc ataggatgtt tgcttgttgt ctgcacgggt
      961 acaatgatag gttttgtttt gtcgggtagg ttatacaaac gcagagattt tctgaaatca
     1021 ttcacggaat ttatatcatt gcttgcaaca aatcttcgct nnnnnnnnnn tacagcggtg
     1081 atgatatctt cactctggtt aatttctgtg cagaaaattc aagccttgat ttattgcttt
     1141 tttcagaatg tgacagacct tttgacgaat tgtggcttga aagggtaaaa caattatctt
     1201 ctgaaatacc gctttcaaaa tccgacattt caatgctgaa cgatttcgga ggtcagctcg
     1261 gaaaaaccga tacagagggg cagttaaaac atcttgaact ctatgaggtg tcattttcaa
     1321 aacagctttc atcagccctt gatgccataa caaaaaaatc aaagctatat aaaacaatgg
     1381 gattttttgc aggaagtgca atcgccctta tgatgattta gaggtagtaa tggatgttga
     1441 gcttattttc aaaattgccg ctgtcggcat aattgttgcg gttcttaccc aacttctcat
     1501 acgaagcggt cgagaggagc aggcaatgct gacctcgctt gccggactta tagttgtgtt
     1561 gacgctgata ataacgcaaa tcagcactct gttcaacaca ataaaacagc ttttcggttt
     1621 ttaatatgaa tattctgtca atttccgtgc ttgccgttgt gaccgtaata atctgcgtaa
     1681 tgctcaaacc gaaaaatgcg gaaatctccg taatgctcgg tataagctgt tccgttatta
     1741 ttttaatcgg cgtgcttact caggtttcgg ctatcgttac aacaatcaat caaatcatag
     1801 cgtcatcggg aataagcatt gactacatag taattctgct gaaatcaatc ggaatatgcc
     1861 ttgtcaccga gttcgccgtg aatacctgca aggattcggg cagtcagtcg cttgcgtcaa
     1921 atgtttcgct tgccggaaaa attatggtca cggtaacggc acttccgctt tattcggata
     1981 ttttaaaaac cgtgttgtca cttgcaggca attgaggtga agaagtgaaa aaaagtgttg
     2041 taataattac tgtcgtgttt gtgcttatcg ccctttcatg tgtctgtgca aacgctgaag
     2101 aaggtgaaat gatttatgat atgtcaaatg tttttgattc gctgtccgac gatataaagc
     2161 aaagccttca gaatatgggc gttgatttgg gggatttttc aaagcttgcc aacctgtctt
     2221 ttgaaaatgt aatgggcgaa attctctcgc ttgcactcgg caacatttct tcaccgctca
     2281 aaggtcttgt caatataacc gccctgcttt tgctctgttc cgtaatcagc gcctacaacg
     2341 gttcgctctc ctgcgatatt tcaaactcgt tgaatataac cgcaacgctc tgtataacct
     2401 gtgccgcagt agtccccgct gtcggagtca tcgcatcaac gggttcggta atctgcaccg
     2461 catcaaatat aatgcttgcc tatgttcctg ttatggctgt aattatggca agctcgggtt
     2521 ctgtggcagg ttcggcatca tactatgccg taatgatagg ggcaggggag ggtgtgtcac
     2581 agctttcatc aaaaataatc gttcctctgc tcaatatgtt cctcggtctg tcaataacat
     2641 catccgtatc gccgtccgta aatctcagcg gttttatgtc aatgatttca aagagcgtaa
     2701 agtggctttt gggctttgct atgacaatct tcacagccgt gctgacattc agacagctta
     2761 taagcgtttc ggttgacaat gtttcaaccc gtgctgtaag gtttactctc aattcattta
     2821 tcccgatagt cggttcggca ctttcggatg cctacaaggt agttcagggc agtatcggac
     2881 ttttgaaatc gggaatagga ataatagtga ttctgtccgt tacaattgtg tttatgccag
     2941 tgattttgca gggagtaatg tggatgttca ccctttggat cggcaaatca acggcagagg
     3001 ttctcaatct aaacggtccg gcaaaactgc ttgaaaacat atcgtctgtc ttttcaactc
     3061 tcatagctgt tttgctgtgc attatgtcaa tttacattct ttcaacggcg gcagtaatta
     3121 tgcttggcgg aggttcatca tgagcggact ttttacggtt gttgttgtaa tgtgtgcgtc
     3181 tgcgttaata tgctcgcttg ttcagacctt tgttacagac ggcagtacaa agaaaatcat
     3241 cagccttgtt ttaggcgctt ttattatctg ctgtatggtt gttcccgtaa aaaatgccgt
     3301 atcgtcattt aataccgata ttgaggaaaa gcaaaattct gttaaagaaa tatcaactga
     3361 tgatgaggca tacagcaatg ccgttataaa gcagacggag ataaatcttg aaactgcatt
     3421 aaaaaatctt cttttacaga ataatataac aatcaaatcg tgtgacataa ttttgagcag
     3481 agttgaaaac aacggcataa tcatagcgtc aatcagcata tatatagaca aagaatttgt
     3541 tcggtacaca aatcttgtat cgtcaattgt ttatgacaat ttcggaatta caccaaacat
     3601 tatgacggag tgaattatgg aagacaaaac aagttcaaag ctgaaatcaa tgctcgacag
     3661 taaaaaaata agaaatatca taatcgttgt cggtcttatc ggtattggtt taatatttct
     3721 ttcaaactgg gttgatttcg gttctttgac atcaggaaaa ggagatgaag cattttccgt
     3781 aaaaacatac agcacaaaaa tagaaaatga tttgcagacg gtgatttcaa aaattgaggg
     3841 agcaggcaag acggaagttt tgctcacaat ggaaaattcc gttgaatatg tttatcttga
     3901 caacagcacc accaaaacaa aggaaattga acctttgata agaggagtgc ttgtagtgtg
     3961 tgaaggcggt gatgatcctg ttgtggttga gcgtgtcacc gatgcagtca caaaagcgct
     4021 tgatatttca acagcaaaag tatgtattac aaaattatca gaataaaggt cgggagtaat
     4081 atgaaatcta agaaaaaata tatcgttttt gcatcacttg tgcttgcact ttcagcggct
     4141 gtgtacataa actggcagct ttcgggagct aaaactcctc aaagtgccaa agaactcgga
     4201 gcggcatcat atgtcagtgc aaccgcttcg gcgtcggatg atgaggtaca gcagacggcg
     4261 gcactttcaa acgagagcaa aagctatttt tcggccgaaa ggacaaagcg gcaggcaact
     4321 caggataaaa taattgatga tgcaaaagaa attttcaatc ttgaaagctc gtctgatgcc
     4381 gacaaaagcg aggctgaaaa gaatgttgaa gagcttctca aaacctttac aattcaggac
     4441 agcattgaaa gtattgtcaa agccaaagga ttttcagaat gcctttgcta tataagcgat
     4501 caaggtgtgt cggtaattgt tccgaaatct cagcttgacg atacatctgt tctgattatt
     4561 gacgatgccg tggttactca ttatgaggtt gattatgacg atatatcggt aataggggca
     4621 taaaattagc agagggcata gtgtcctctg cttttttgtg cataaaatca aacgcattcg
     4681 gttgcgagga aagttaaaaa agtaaatgat ttttaacgga catctttgtt aattattgtt
     4741 tacattcatt ttcagttatg gtataatctt gtaagtaaat tagggagtta ctctgattta
     4801 aatcttaatg atatgatttt agtgatattg taatttaacg aaaagttggg tttattatga
     4861 ataacgaaat taacaatgaa ttaaatgaaa ttgaagtaaa tgacgctgaa acaagtgaca
     4921 tctttgaaga agaggttaat ctcacaagaa gtgagtcaag agagcaggct tttatgcttt
     4981 tgttttcaaa gtcttttgac gatgagcctc ttgaagaaac aatagatgat aattcggaaa
     5041 tgtttgtagg cggtgtttgc gcatatgctc aggctgtagt ttccggcatt gaggacaagc
     5101 atgaggagat tgacgaaatc attgcaagcc acctgaaaaa gggctggact ctttcaagaa
     5161 tttcaaagcc gtcgcttgct attcttcgcc ttgcaatcta tgagatgaag tatctcgaca
     5221 atgttccgca gagcgtttca atcaacgagg ctgttgaact tgccaagaaa tatacaattg
     5281 acgagagcaa atttgtaaac ggtattctcg gcacatattc ccgtgaattt tcgggaaagt
     5341 cggagaagtg atatgagcct ttttctcgga attgacactt caaattacac aacatcgacc
     5401 gccctttacg acagcaaaac gggcgaaatg gttcagcaga aaaaacttct tcccgtaaaa
     5461 gaaggtcagc tcggacttcg tcagagtgac gctgtgtttc accacacagc acagctgcac
     5521 acgcttattg aagaactttt gaaagatgtt gatacaagca aaatagccgc aattgcggcg
     5581 tcaagccgtc caagacctgt tgacggctca tatatgccgt gtttcactgt tggagtgaac
     5641 acggcaaaaa atttatcttc cgcaatgaaa atacctcttt acacattttc acatcaggag
     5701 ggacatatct cggcggcatt gttcggctcg cagagaacag atttgtttga taagcagttt
     5761 atcgctttcc atgtcagcgg aggtacaacc gaggcagtat ttgccaaagg attcggcacg
     5821 ggcttttccg ttgaacttgc ggcacacaca ctcgacctta atgcaggaca ggcgattgac
     5881 cgcacaggac ttatgctcgg tttaaaattt ccgtgcggtc ctcagcttga acagcttgcc
     5941 cttaaaaata cagaaaaaat caaggtgaaa ccaactctca aaggttgcga ttgttgcctg
     6001 agcggagttg aaaatctttc taaaaa
//
LOCUS       DAOMVE010000118         5901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3638_length_5901_cov_4.075436, whole genome shotgun sequence.
ACCESSION   DAOMVE010000118 DAOMVE010000000
VERSION     DAOMVE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3638_length_5901_cov_4.075436"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1269
                     /locus_tag="PLS64_07135"
     CDS             4..1269
                     /locus_tag="PLS64_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRL43631.1"
                     /translation="MNKPLADRIRPKTIDDIVGQKHLIAPDRPLRKIIESGDIPNLIF
                     YGPSGVGKTTLATYIAKKTNRTLKKLNGTTASTADIKEIVAQLNTFSGLNGILLYLDE
                     IQYFNKKQQQTLLEYIENGSITLIASTTENPYFYVYNAILSRSTVFEFKPVDQDEVEK
                     AVYRASDFLAEESGTDIEMPEECAKKIASGCGGDVRKAMNAVELSVIATDEIDGKKVV
                     RLETVEQLVQKSAVRYDREGDEHYDIISAYQKSMRGSDPDAALHYLARLLEAGDLPSA
                     CRRLMVCASEDVGLAYPQLLPIVKSCVDIAQAVGLPEARIPLADAVVMVCNAPKSNSA
                     YMGINRALADIRTGNSGPVPRQLQNRHCDGDDNPNKGQFYLYPHDFPNHYVEQQYLPD
                     ALKDKKYYEYGRNKNEQAFKAYWDKIKKK"
     gene            1548..3194
                     /locus_tag="PLS64_07140"
     CDS             1548..3194
                     /locus_tag="PLS64_07140"
                     /inference="COORDINATES: protein motif:HMM:NF028048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starch-binding protein"
                     /protein_id="HRL43632.1"
                     /translation="MVKRQHKIMCLVLCLMLAVSTVFVGVVSASAATGDTVYVRVNNG
                     WSKVNCYMWTDGLGNNASWPGKAMTKVEDGVYSYTLDKDYTKVIFNDGSNQTKDLTYP
                     GHGKIYDLSKGTWSDYAGAPSQGPTVQTPTSATTATTATTATTPSGEGTTVYLNNEAG
                     WATPVVYMWNGSTDSNGSWPGVKMTSIGGNIYQYTASKSYAKCIFSNSGQSQTSDLTA
                     KDGYIYNNKTNQWSVYDTSPLQVKEFTADPASNIYTGTDVQLSATAANKSGAAVSYKF
                     SVTNAQGGTSTLSDFSSAKSVTWTPTVAGEYTITFDFKDTDGNTNNRTMTLEVKDDSA
                     LVKPIIKSVTPANLNLIKANSTATVTVKAGGGKTGTNLLFYKYVVTDPNGAQNTPYYT
                     LNNIYTFVPTMKGEYKVNVYVQSSDNSTINKTYTYTAADDVTEPTTVVVPTTAPQPTT
                     VQPTTVKPTTPQPTTVKPTTPQPTTVQPTTAPERLRGDVDGDGEVTVMDSTYLQKSIV
                     GLSDVPALETLDLKICDLDGDGTISVKDATIIQKIVVNLE"
     gene            3233..5647
                     /locus_tag="PLS64_07145"
     CDS             3233..5647
                     /locus_tag="PLS64_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL43633.1"
                     /translation="MTRIKKLCALVLAVMMVVTAGVISVNAAQSSDSDAVSADTTETG
                     ITIHVRMSDGTQPYVYLWNSLPTNSAMSKSYPGEKMTKGDKWYNYHVADVTKVNAIVT
                     DANGKQYSSEKKLESAASADWYCENGKWSKYNPDEPDPIGSVDMREETIYFVMTTRFY
                     DGDTGNDVHCWDDGTAGNGDDDPAWRGDFKGLGEKLDYIKALGFSAIWVTPVVTNGSG
                     YDYHGYHAMDLSTVDARYESSDYTYEDLIHDAHQKGMKIIQDVVLQHTGNFGEAHFCD
                     LFTKDTTKDLGNLQESLIPTQYLLDTYGLKSPEEYWAQKPGVQYQQRLNLMKNVTYSG
                     DNGNSTGPQPAAQDFSMNKISQSDTYNPNNYYHTGYFQSPNYDDWTTKFSQIAGDCVD
                     LNTENPAVAEYVVDSYSKYMDMGVDGFRVDTVRHIPRVSLNIMFNDQLMDAAKAAGKP
                     NFYMFGEICTRYTSVWYRGHAEESTPYYTWKESNSKWADSWNWGTSASEINDNMNLVL
                     QHYLEEDNYNGDMDSTQPKSDNAYLDGITYHGSDRSMASGMDAIDFQMHRMFGSAKNA
                     YNFAVNNDQYYNDATYSVMYVDSHDYAPEQPDETTRFTGGTQTWAENMDLMFTFRGIP
                     CVYYGSEVEFKKGELIDKGTLISLENSGRAYFGDYLEGTVKATDFSEYTASGTVADTL
                     ASPLSKHLSKVNAIRRAIPALQKGQYTASSTYVTGGDMSYVRRYTDDNTDSLALVSIS
                     SGATFKNIPNGKYVDAVTGDVKYVTDGTLTVPELAKANMRVYVCCASGFTGIDGQIGG
                     DSAYAK"
BASE COUNT         1816 a         1281 c         1282 g         1522 t
ORIGIN      
        1 attatgaaca aaccgcttgc ggacaggatt cgtccaaaga caattgatga cattgtggga
       61 caaaagcatc ttattgcacc cgacagacct ttgcgtaaaa ttattgaaag cggagatatt
      121 ccgaatctta ttttctacgg tccttcgggg gtcggaaaaa ctacgcttgc cacatatatt
      181 gccaaaaaga caaaccgtac actcaaaaag cttaacggca caacggcttc aactgccgac
      241 ataaaggaga ttgttgcaca gcttaacact ttttcggggc ttaacggaat tctgctctat
      301 cttgacgaaa ttcagtattt taataaaaag caacagcaga ctttgctcga atacattgaa
      361 aacggcagta tcacgctgat tgcgtcaaca accgaaaatc cgtattttta tgtatataac
      421 gcaattctga gccgttcgac tgtttttgaa tttaagccgg ttgatcagga tgaggttgaa
      481 aaggcggttt accgtgcaag tgattttctt gccgaggaga gcggtacgga tattgaaatg
      541 cccgaagaat gtgcaaagaa aatagcatcg ggttgcggag gagatgttcg caaggctatg
      601 aatgccgttg agctttccgt gattgcaaca gatgaaattg acggaaaaaa ggttgtaaga
      661 cttgaaaccg ttgaacagct tgtgcagaaa agtgcggtca gatatgaccg tgagggcgat
      721 gagcattacg atattatttc ggcttatcag aaaagtatgc gtggaagcga tcccgatgcc
      781 gcacttcact atcttgcaag actgcttgag gcaggcgacc tgccgagtgc gtgtcgcaga
      841 cttatggtgt gtgcgagcga ggatgtggga cttgcgtatc cacagctttt gccgattgta
      901 aagtcatgcg ttgatatagc acaggctgtc ggacttcccg aggcgagaat tccgcttgcc
      961 gatgccgtgg ttatggtgtg caatgcccca aaatccaact cggcatatat gggtataaac
     1021 agagctttgg ctgacataag aacgggtaac tcgggacctg tacccagaca attgcagaac
     1081 aggcactgtg acggagatga caatccaaac aagggacagt tctatcttta tccccacgat
     1141 tttccgaacc attatgttga gcaacaatat ctgcccgatg ccctaaagga caagaagtat
     1201 tacgaatacg gcagaaataa aaacgagcag gcgttcaaag cctattggga caaaatcaaa
     1261 aagaagtaat caaatgaaaa ttttttgaca tgaaactgca aataaagtca gatgttgcta
     1321 accaaacatc tgactatttg cttttattat cctgtttttt tgctaattat tgtttcattg
     1381 tctattatgc acaatttgaa tgtgaataat ttgtgacgca agaaatttta ttattcttta
     1441 aaggaaaatt attagcggtt caatattgta gaattaaaga gtaattgtgg agtagtgttg
     1501 agtagcttca cacgtgcaac aaaaaaataa ttctggagga aatcaaaatg gtaaaaagac
     1561 aacacaaaat catgtgcctt gtgctttgcc ttatgctggc ggtttcaacc gtattcgtcg
     1621 gtgtagtcag tgcttctgct gcaacaggcg atactgttta cgttagagta aacaacggct
     1681 ggtcaaaagt taactgctat atgtggactg acggcctcgg caacaacgca tcttggccgg
     1741 gcaaggcaat gacaaaggtt gaagacggcg tatattccta cactcttgac aaggattata
     1801 caaaggtaat cttcaacgac ggctcaaatc agacaaaaga ccttacatac ccgggtcacg
     1861 gcaaaatcta tgacctttca aagggtacat ggtcggatta tgcaggtgct ccttcacagg
     1921 gtccaactgt tcagacacct acaagcgcaa caacagcaac aactgctaca acagcaacaa
     1981 ctcccagcgg tgaaggcaca actgtttacc tcaacaacga ggcaggttgg gcaacaccgg
     2041 tagtttatat gtggaacggc agtactgaca gcaacggctc atggccgggc gtaaagatga
     2101 caagcatcgg cggcaacatc tatcagtaca ccgcatcaaa gtcatatgca aaatgcatct
     2161 tcagcaacag cggtcagtca cagacatcag accttactgc aaaggacggc tatatataca
     2221 acaacaagac taatcaatgg tctgtttacg atacaagccc tctccaggtt aaggaattta
     2281 ctgctgatcc tgcatcaaac atctacacag gtacagatgt acagctttct gcaactgctg
     2341 ccaataagag cggcgcagca gtaagctaca agttctctgt tactaacgca cagggcggaa
     2401 catcaacact ttccgacttc tcttctgcta agtcagtaac atggactcct acagttgcag
     2461 gtgaatacac aattacattt gactttaagg acacagacgg taacacaaac aaccgtacaa
     2521 tgactcttga agttaaggac gactcagcac ttgttaagcc aatcatcaag agcgttactc
     2581 ctgcaaacct taaccttatc aaggctaact caactgctac tgtaacagtt aaggcaggcg
     2641 gcggtaagac aggtactaac cttcttttct acaagtatgt tgtaactgat cctaacggtg
     2701 ctcagaacac accttactac acactcaaca acatttacac attcgttcct acaatgaagg
     2761 gtgaatacaa ggttaatgtt tatgtacaga gttctgacaa cagcacaata aacaagactt
     2821 atacttacac agctgcagac gatgttactg agcctacaac agttgttgtt cctacaactg
     2881 ctcctcagcc aacaacagtt cagcctacaa ctgttaagcc tacaactcca cagccaacaa
     2941 ctgttaaacc aacaactcct cagccaacaa cagttcagcc tacaacagct cccgaaagac
     3001 tccgtggcga tgttgacggt gacggagaag taactgttat ggactcaaca tatcttcaga
     3061 agtcaattgt aggactttcg gatgttccgg ctctcgaaac acttgacctc aaaatttgcg
     3121 accttgacgg tgacggtaca atcagtgtta aggatgcaac aatcattcag aaaatcgttg
     3181 taaaccttga ataatccaaa cattaagtaa aagaaagatt ggagatatac ctatgacaag
     3241 aataaagaag ctgtgcgcac ttgttcttgc tgtgatgatg gttgtaaccg caggagtaat
     3301 ttctgtaaat gcagctcaga gttccgactc tgacgctgta tctgcggata caacagaaac
     3361 aggcatcaca attcatgtaa gaatgagtga cggtacacag ccatatgtat atttgtggaa
     3421 ctcgctcccc acaaacagcg ccatgtcaaa gtcttacccc ggtgagaaaa tgacaaaggg
     3481 tgacaagtgg tataactacc atgtagcaga tgttacaaag gtaaatgcca ttgttacaga
     3541 cgctaacggc aagcagtatt cttcggagaa aaaacttgaa tctgctgcaa gcgcagactg
     3601 gtactgtgaa aacggtaaat ggtcaaaata caaccctgac gagccggatc cgattggcag
     3661 cgttgatatg cgtgaagaaa caatttactt cgttatgaca acccgtttct atgacggcga
     3721 tacaggcaac gatgttcact gctgggatga cggcacagcc ggcaacggtg atgacgaccc
     3781 tgcatggcgt ggtgacttca agggtcttgg cgaaaagctc gactacatca aggctctcgg
     3841 cttcagcgca atctgggtaa cacctgttgt tacaaacgga tcaggctatg actaccacgg
     3901 ctatcatgct atggatttgt caacagttga tgctcgttat gaaagctcag actacacata
     3961 tgaggatctt attcatgatg ctcaccagaa gggcatgaag attattcagg acgtagttct
     4021 tcagcacaca ggtaactttg gtgaagcaca cttctgtgat cttttcacaa aggatacaac
     4081 taaagacctt ggcaaccttc aggagtcatt gattcctaca cagtatcttc ttgatacata
     4141 cggccttaag tcacctgagg aatactgggc tcagaagccg ggtgttcagt atcagcagag
     4201 acttaatctt atgaagaatg ttacttactc aggcgacaac ggtaactcaa ccggtcctca
     4261 gcctgcagct caggacttct ctatgaataa gatttcacag agtgatactt acaacccgaa
     4321 caactactat catacaggct atttccagag tcctaactat gatgactgga caacaaagtt
     4381 cagccagatt gcaggtgact gcgttgacct taacacagag aaccctgcag ttgcagaata
     4441 tgttgtagat tcatattcaa agtatatgga tatgggcgtt gacggcttcc gtgttgatac
     4501 cgttcgtcac attccgagag tatctctcaa cattatgttc aatgatcagc ttatggacgc
     4561 tgctaaggca gcaggcaagc ctaacttcta tatgttcggc gaaatctgca caagatacac
     4621 atcagtttgg tatcgtggcc atgccgagga gtcaactcct tactacacat ggaaagagtc
     4681 taactctaag tgggcagaca gctggaactg gggcacatca gcttcagaaa tcaacgacaa
     4741 catgaacctc gttcttcagc attatcttga agaagataat tacaacggtg atatggattc
     4801 tacacagcca aagagcgaca acgcttacct tgacggtatt acatatcacg gttccgaccg
     4861 ttctatggca tcaggcatgg atgcaattga cttccagatg cacagaatgt tcggtagcgc
     4921 taagaacgct tataacttcg ctgttaacaa cgaccagtat tacaatgatg caacatacag
     4981 cgtaatgtat gttgattcac acgactatgc tcctgaacag ccggatgaaa caactcgttt
     5041 cacaggcggt acacagacat gggcagagaa tatggatctt atgttcacat tccgtggtat
     5101 tccttgcgta tactatggtt cagaagttga gttcaagaag ggtgagctta ttgataaggg
     5161 tacacttatt tctcttgaaa actcaggtcg tgcatacttc ggtgattatc ttgagggtac
     5221 tgtaaaagct actgacttca gtgagtacac agcatcaggc actgttgctg acactcttgc
     5281 aagcccgctc agtaagcacc tttcaaaggt taatgctatt cgtcgtgcta tccccgctct
     5341 ccagaaggga cagtacacag catcaagcac atatgtaaca ggcggtgata tgtcttatgt
     5401 tcgccgttat actgacgaca acacagacag ccttgcactc gtttcaattt cttcaggtgc
     5461 aacattcaag aacattccta acggcaagta tgttgacgct gttacaggtg atgttaagta
     5521 tgttactgac ggtacactta cagttccgga acttgctaag gctaacatga gagtttatgt
     5581 atgctgcgca agcggattta caggtatcga cggtcagatc ggcggagatt ctgcttacgc
     5641 taaataatgc ggaatattcc acactcaaca cgattctgat tctgtaatca aaattaatta
     5701 cggcactccg agtaaaatcg gggtgccgtt ttttaattgt tattttattt gttcgtaata
     5761 ttttaaaaaa tatgcttata ataaaagtgt atccgaagtg ttatgtcagc ttaaaaatgc
     5821 ttgacaaaaa atcttttgaa cgaaaataca aatatttcac gcttaaatat ttctatgatc
     5881 ttctcggtat agaataagaa t
//
LOCUS       DAOMVE010000119         5644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_3933_length_5644_cov_4.052603, whole genome shotgun sequence.
ACCESSION   DAOMVE010000119 DAOMVE010000000
VERSION     DAOMVE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3933_length_5644_cov_4.052603"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(406..1608)
                     /gene="tuf"
                     /locus_tag="PLS64_07150"
     CDS             complement(406..1608)
                     /gene="tuf"
                     /locus_tag="PLS64_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRL43634.1"
                     /translation="MAREKFERNKPHVNIGTIGHVDHGKTTLTAAITKVLNLEGDADF
                     VDYANIDKAPEERERGITINTAHVEYETDKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKTDQVDDPELLELVEMEIRDLLNE
                     YEFPGDDTPIIKGSAYLALTSTSKDPNAPEYKCIHELMDAVDEYIPTPDRKADQPFLM
                     PVEDVFTITGRGTVATGRVERGQIKTGEEVEIVGLTDEKRKVVVTGLEMFRKTLDFAE
                     AGDNVGVLLRGVQRTEIQRGQVLAKPGTIHPHTKFRGQVYVLTKDEGGRHTPFFNNYR
                     PQFYFRTTDVTGTISLPEGVEMCMPGDNVEMDVELITPIAIEVGLRFAIREGGRTVGS
                     GAVIAINE"
     gene            complement(1724..3823)
                     /gene="fusA"
                     /locus_tag="PLS64_07155"
     CDS             complement(1724..3823)
                     /gene="fusA"
                     /locus_tag="PLS64_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRL43635.1"
                     /translation="MPRQVSLEKTRNIGIMAHIDAGKTTTTERILYYTGVNHKIGETH
                     DGASTMDWMVQEKERGITITSAATTAFWFGSKHQYDKHRINIIDTPGHVDFTVEVERS
                     LRVLDGSVTVLCAKGGVEPQSETVWRQADNYGVPRMVYVNKMDIMGANFYHVVQMMKD
                     RLKCNAVPIQLPIGAESDFRGIVDLVTMKAEIYHNEDGTDYSEEEIPEEMQDLANEYH
                     EKLIESVAELDEDLMEKYFGGEELTIDEIKSTIRKATIDNTMVPVTCGSSYRNKGVQM
                     LLDAIVDYMPAPTDVPAIKGVDPETGDEVERPSSDKEPFAALAFKIATDPFVGKLCFF
                     RVYSGTVSAGTTVYNSVKDTNERLGRILQMHANDRKDIDCVYAGDIAAAVGLKNTTTG
                     DTLCDEKAPVILESMEFPDPVIRVAIEPKTKAGQEKMGIALAKLAEEDPTFKAYTDEE
                     TGQTIIAGMGELHLEIIVDRLLREFKVEANIGAPQVAYKETIRKEASVDEKYARQSGG
                     KGQYGHVKINIYPNKDKGYEFVNNIVGGAIPKEYIPAVDQGIQGAMLSGVVAGYNVVD
                     VKVELYDGSYHEVDSSEMAFKIAGSMAFKNAMKKADPVLLEPIMKVTVITPEEYLGDV
                     IGDLNSRRGMIQSMEADNGVQRVIAHVPLSEMFGYATDMRSKTQGRGQYVMEPDTYAE
                     VPKNIAEKIMSERAKKD"
     gene            complement(3855..4325)
                     /gene="rpsG"
                     /locus_tag="PLS64_07160"
     CDS             complement(3855..4325)
                     /gene="rpsG"
                     /locus_tag="PLS64_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRL43636.1"
                     /translation="MPRRGSIAKRDVLPDPLYNSKLVTRLINNIMYDGKKGVAQKIVY
                     GAFDIIAEKTGNNPLEVFEQAMENVMPVLEVKARRVGGATYQVPMEVRPERRQTLGLR
                     WISTYSRARSEKTMKERLAGEILDATNSAGGAFKKREDTHKMAEANKAFAHYRW"
     gene            complement(4483..4902)
                     /gene="rpsL"
                     /locus_tag="PLS64_07165"
     CDS             complement(4483..4902)
                     /gene="rpsL"
                     /locus_tag="PLS64_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRL43637.1"
                     /translation="MPTFNQLVRKGRQVSEKKAKAPALLKGWNSKKRVAIDQSSPQKR
                     GVCTAVKTATPKKPNSALRKIARVRLSNGIEVSSYIPGVGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGTLDAQGVSGRMQSRSKYGAKRPKKGK"
BASE COUNT         1564 a         1277 c         1153 g         1650 t
ORIGIN      
        1 tgtgaatttg tataccatgt ccttgcacag gtggttacca tgttgcactt gacacgatga
       61 acgcccctac gagattgtgc cattatagaa tttgttttaa catataattt gtgatattac
      121 ctgcataagg atgatatctg cccctacaaa aaacacaaaa agagccgata caaatgtaat
      181 ggatgttgtg tataatctgt aggggaaaac ctcgttctcc cgcagataac agcacataca
      241 ttatgataat tacaaagcat ataaatatcg caaagtcaag ttttatctgt agcagcgaac
      301 cgagttcggc aatcgtatca tgtttgcaca cgatgcgaac acaaaaagag ccgatacaaa
      361 tgtatcggct ctgaatgtta gaaaataaac gataatcggt taaaattatt cgttaatagc
      421 gataactgcg cctgaaccta ctgttctgcc gccttcacga atagcgaaac gaagaccaac
      481 ttcgatagcg ataggagtga taagttcaac gtccatttca acgttatcac caggcatgca
      541 catctctacg ccctctggga gtgagattgt acctgttacg tcagttgttc tgaaatagaa
      601 ctgtggacga tagttgttga agaatggagt atgacggcca ccttcgtcct tagtaagaac
      661 gtaaacctgg ccacggaact ttgtgtgtgg gtgaattgta cctggcttag caagtacctg
      721 acctctctgg atttcagttc tctgaacacc acggagaaga acacctacgt tgtcgcctgc
      781 ttcagcgaag tcaagagtct ttctgaacat ttcaagacct gtaacaacaa cttttctctt
      841 ttcgtctgta agaccaacga tttcaacttc ttcaccagtc ttaatctgac ctctttcaac
      901 tctaccagtt gcaacagtac cacgacctgt gattgtgaat acgtcctcaa caggcataag
      961 gaatggctgg tcagccttac ggtcaggagt tggaatatac tcgtcaacag cgtccataag
     1021 ctcgtggatg cacttgtact caggagcgtt aggatcctta gatgtgcttg taagagcaag
     1081 ataagctgaa cccttgatga ttggtgtatc atcgcccggg aactcatact cgttgaggag
     1141 gtcacggatc tccatctcta cgagctcgag aagctcagga tcgtcaacct ggtcagtctt
     1201 gttcatgaat actacgatgt aaggtacacc tacctgacga gaaagaagga tgtgctctct
     1261 tgtctgtggc ataggaccgt cagcagctga tacaacgagg atagcgccgt ccatctgagc
     1321 agcacctgtg atcatgttct ttacatagtc agcatggcct gggcagtcaa cgtgtgcata
     1381 gtgacgcttg tctgtttcat actcaacgtg agctgtgtta attgtgatac cacgctctct
     1441 ctcttcagga gccttatcga tgttagcgta atcaacgaaa tcagcatcgc cttcgaggtt
     1501 gagaaccttt gtgatagcag cagtaagtgt tgttttacca tggtcaacgt ggccgattgt
     1561 accaatgtta acatggggct tatttctttc aaatttttct cttgccattg gaaatttcct
     1621 ccttattgtt gtaaaaagaa tttacaatag atttaatcat attgtatcaa ttttttctaa
     1681 aaaaatcaat acatttttga aaaaagtgct ttaaaaggta tgattagtcc tttttagctc
     1741 tttcactcat aatcttttca gcaatgttct tcggaacctc tgcatatgtg tcaggctcca
     1801 taacatactg accacgaccc tgagtcttgg aacgcatgtc tgttgcgtaa ccaaacatct
     1861 ctgaaagcgg aacatgagcg ataactctct gaacgccgtt gtcagcttcc atgctctgga
     1921 tcataccacg acgagagtta aggtcgccga ttacatcgcc gaggtattcc tcaggagtga
     1981 taacagtaac cttcatgatc ggttcgagaa gtacaggatc tgccttcttc atagcgttct
     2041 tgaatgccat tgaaccggca atcttaaatg ccatttctga tgagtcgact tcatgataag
     2101 aaccatcata aagctcaacc ttaacatcaa ctacattgta gcctgcaaca acaccgctga
     2161 gcattgcgcc ctgaataccc tggtcaacag ccggaatgta ttcctttgga atagcaccgc
     2221 cgacaatgtt gttaacgaac tcatagccct tgtccttgtt aggatagata ttgatcttaa
     2281 cgtgaccgta ctgaccctta ccacctgact gacgagcata cttttcgtca acagaagctt
     2341 ccttgcggat agtttccttg tatgcaacct gtggagcacc gatgtttgct tctaccttaa
     2401 attcacggag aagacggtca acaatgattt cgaggtggag ttcacccata cctgcgatga
     2461 ttgtctggcc tgtttcttcg tctgtataag ccttgaaagt aggatcttct tctgcaagct
     2521 ttgcaagagc gatacccatc ttttcctgac ctgcttttgt cttcggctca atagcaacac
     2581 gaattacagg atcagggaat tccatagact caaggataac aggagccttc tcatcacaga
     2641 gagtatcacc tgttgttgta tttttaagac caacggcagc agcgatgtca cctgcgtata
     2701 cgcagtcgat atcttttctg tcgttagcgt gcatctgcaa gatacgaccg agacgctcgt
     2761 tggtgtcctt aactgagttg taaacagttg tgccggcaga aactgtaccg gagtaaactc
     2821 tgaagaagca gagcttacca acaaacgggt cagtagcaat cttgaatgcg agagctgcga
     2881 atggctcttt atctgatgac ggtctttcaa cctcatcgcc tgtttcaggg tcaacaccct
     2941 tgatagcagg tacatctgtc ggagcaggca tataatcaac gatagcatca aggagcatct
     3001 gaacaccctt gttacggtaa gaagaaccgc aggttacagg taccattgtg ttgtcaatag
     3061 tagcctttct gattgtgctc ttgatttcgt cgattgtaag ctcttcgccg ccgaagtatt
     3121 tctccataag atcctcatcg agttcagcaa ctgattcgat aagtttctca tgatactcgt
     3181 tagcgagatc ctgcatctct tccggaattt cctcttcact gtagtctgtg ccgtcttcgt
     3241 tgtgatagat ttcggccttc atagtaacga ggtcaacgat gcctctgaaa tcactctcag
     3301 caccgatcgg aagctgaatc ggaacagcat tacatttaag tctgtccttc atcatctgaa
     3361 caacatggta gaagttagcg cccatgatgt ccatcttgtt tacataaacc attctcggaa
     3421 cgccgtagtt atcagcctga cgccatactg tttcagactg aggctcaacg ccgccttttg
     3481 cgcagaggac tgttacagaa ccgtcaagaa cacgaagtga acgctcaact tcaacagtga
     3541 agtcaacgtg accgggggtg tcgatgatgt tgattctgtg cttgtcatac tgatgcttgc
     3601 tgccgaacca gaatgcagtt gtagcagcag aggtgattgt gataccacgc tctttttcct
     3661 gaaccatcca gtccattgtg gaagcaccgt cgtgtgtctc accgatttta tggtttacgc
     3721 cggtataata caggatacgc tcagttgttg tggttttacc ggcatcaatg tgagccatga
     3781 taccgatgtt tcttgttttt tcaagtgaaa cctgtcttgg cataaaatcc tcctaataaa
     3841 cgctgatgat aaaattacca tctgtaatga gcgaaagcct tgttagcttc agccatctta
     3901 tgagtgtctt cacgcttctt gaaagcaccg ccggcactgt ttgtggcatc aaggatttct
     3961 cctgcaagtc tttccttcat tgtcttttcg ctgcgagctc ttgaataagt agaaatccag
     4021 cggagaccga gtgtctggcg tctttcagga cgaacctcca taggtacctg ataagtagca
     4081 ccgccgacac gacgagcctt aacttcgaga acgggcatta cattttccat agcctgttca
     4141 aaaacctcaa gcgggttgtt acctgtcttt tcagcaataa tatcgaatgc accgtaaaca
     4201 atcttctgag caacgccctt tttaccgtcg tacataatgt tgttgataag acgagttacg
     4261 agttttgaat tataaagcgg atccggtaat acgtcacgct tcgcaataga acctcttctt
     4321 ggcactttgc ttccctcctt cacaataatt gagatatcat aggtactcga aagcgacaaa
     4381 ctcccgtatg ccaacaagac attctaccat aatatatata gtaaaattcc tgccgcatag
     4441 cagaacttaa ataatttaag tcaagagagt tttgtcaatt ccttatttac ccttcttcgg
     4501 acgcttagca ccgtactttg aacggctctg catacggccc gaaacaccct gagcatcgag
     4561 tgtaccacga acgatgtggt aacgaacacc aggcaagtcc ttaacacggc caccacgaat
     4621 aagaacaacg ctgtgttcct gaaggttgtg acctacgccg ggaatatagg aactgacttc
     4681 aataccgtta gaaagtctta ctctggcaat ctttctgagc gctgagttag gctttttagg
     4741 tgtagcggtc tttactgcag tacaaacacc tctcttctgt ggggagctct gatcaatagc
     4801 aacacgcttt tttgagttcc aaccctttaa gagagcaggt gcctttgcct tcttctcgga
     4861 aacctgtctg cccttgcgta ccaactggtt aaatgtaggc attatttgtc ctcctttcaa
     4921 aaaatataag aattgataaa actgctttgc ttttgcaaag ccctgtgaaa catcaataat
     4981 atccccaaaa acgggcgcaa ttacagaatg tcccacgatt aaacattata aactatccgc
     5041 tcactttttg tcaagcattt ttttgtaaaa tttaagcggg cttatgcttg ataaatatta
     5101 tacattccca cgaatatgtt ttgatattat gaaagctgta tattaatcac agcaactagc
     5161 aaaaaattgt ctgcacgaat tgacaacggc tgcacaccgt aatgaaaaag aggctgtaaa
     5221 gaaatgaagt ttaagacttc gttcttcaca gcctccgttt tcatttttta tttaggagag
     5281 aggaaaatat atcaaataaa aacttataca ctgtttaatc gggacgatgt tcgccgttcc
     5341 cttatgttgt gactttatta taccacacca atattgacaa ctgtgtgacg aaaacaagaa
     5401 ttttttcgga attttcaatt tttatcattt gtagtaataa acaatgtatt tgagaattta
     5461 taaaatttgt ttttaaatat aatttctgcc gttactcacg ggagaacgag gttctctcct
     5521 acaaaattgt gcaaatggta gctatactac gctcgggcat aatatgattt atatattata
     5581 aaactgtctt cacgaacaaa aaatcattca gcagacttaa tgattaccgt tgactgtggc
     5641 ggta
//
LOCUS       DAOMVE010000120         5554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_4026_length_5554_cov_3.102928, whole genome shotgun sequence.
ACCESSION   DAOMVE010000120 DAOMVE010000000
VERSION     DAOMVE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4026_length_5554_cov_3.102928"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLS64_07170"
     CDS             <1..307
                     /locus_tag="PLS64_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRL43638.1"
                     /translation="DPFYNAPVVLIVLADKAAPTYLYDGTLVMENLMLAAHDEGLGSC
                     WIHRAKEEFESKEGKEILKSLGIEGDYEGIGHCVIGYVNGEYPKTSPRRENRVFYVE"
     gene            339..>786
                     /locus_tag="PLS64_07175"
     CDS             339..>786
                     /locus_tag="PLS64_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL43639.1"
                     /translation="MTLQQLKYVVTVARTGSMSEASKELFISQPGLTTSIRELETEMG
                     IKIFTRTNKGVVTSNEGERFLGYARQVLEQASLLEGVYKSGTKIKQQFCVSTQHYSFA
                     VNAFVDLIRRFAGEEYDFSLRETETYEIIDDVAKMKSEIGILYLNSF"
     assembly_gap    787..796
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <797..1237
                     /locus_tag="PLS64_07180"
     CDS             <797..1237
                     /locus_tag="PLS64_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRL43640.1"
                     /translation="ALMKIIKSKSLTFTELFTAKPHVFVSTDHPLADKKSVTMEELAD
                     YPYLSFEQGEHNSFYYSEEIFSTVGRTKNIRVTDRATLFNLLIGLNGYTVCSGVIDNE
                     LNGDNITAIPLDEEGNMKIGYIENKKILHSNLAEAYIEALKKHI"
     gene            1338..2450
                     /locus_tag="PLS64_07185"
     CDS             1338..2450
                     /locus_tag="PLS64_07185"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRL43641.1"
                     /translation="MSKQHTPFRFETVGSFLRPDYLKKARLDFENGKITKEELTAVED
                     KAILDLVAKQKKAGIKAITDGEFRRATWHLDFMWGFNGVEHKKTENGVTFHGEQALID
                     DTWLSGEISVDSHPFVEHYKFVKALEDENTVAKQTIPAPAQFFQQFIIPANIETTRKF
                     YSTDEELINDIANGYKKVIKDLYDAGCRNIQFDDCTWGVLVAEGSVNRYGEDADFKSI
                     SEKLLKVNNLAIEGKPDDLVINTHVCRGNYHSAYFSSGAYDPVAEKLFGEENVNAYYL
                     EFDDERSGGFEPLKYVSGDKKVVLGLITTKSPVLEDKQTVINRIHEAAKYIPLDRLYL
                     SPQCGFASCEIGNKLTEEEQWAKLALVKEIAEEVWK"
     gene            complement(2506..3438)
                     /locus_tag="PLS64_07190"
     CDS             complement(2506..3438)
                     /locus_tag="PLS64_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL43642.1"
                     /translation="MKFQRTLCSYKGMTKRNIKVYLKDKTAIIFSMMTQIIILGLYLL
                     FLKQNYVDSMTSMLDGFNIKTEDNLINALVNSWLVSGVIGTSVVTVAMNSLSVMISDK
                     QNKIDYDYNASSVKGSTVVLSYFSGAVINTIVISAILLTAGIAFSCISGSSFPEFTEL
                     LKLYGLVILGSVSAVLILMFVASFFKKNSTYAAFNTLISVAIGFVIGAYIPVSQFSDG
                     VQTFVNLIPGSHIAAMIRNVLVSPCINDISTSLAGADHGMFAAEAEKAFATNLNLFGN
                     EVDFTFMMMYSIGAILIFLILNLISYKFSSKRKD"
     gene            complement(3440..4351)
                     /locus_tag="PLS64_07195"
     CDS             complement(3440..4351)
                     /locus_tag="PLS64_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43643.1"
                     /translation="MSNIIEVKNLVKNYKDVHAVNDISFTVEEGSFFAFLGINGAGKS
                     TTINILCTVLEKTSGNVKIGGYDLDKEKNKIKELIGIVFQGSVLDKQLTVKENLESRA
                     SYYGLNKKEIAKRIESLTQTFDLSEILKRKYGTLSGGQRRRVDIARALINRPKLLFLD
                     EPTTGLDPKTRLQVWEIIHNLRKETGLTVFLTTHYMEETVDCDNVVIIDSGKIVANDS
                     PHNLKKDYSSNSLVWYTEENSENDNLLKADNLEFERVGDAYKIKIKNSRQALEITNKH
                     TEIVDFEIIKGNMDDVFLTVTGKRLGD"
     gene            complement(4467..4913)
                     /locus_tag="PLS64_07200"
     CDS             complement(4467..4913)
                     /locus_tag="PLS64_07200"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 family protein"
                     /protein_id="HRL43644.1"
                     /translation="MNDSLKEIIKEVIKDFFIITVAMLFVVSLANSIALIEYYPPDFP
                     WVVMGTAAVTSIPTLLFYFKEEPTKLQARIRIVIHFCLIQAIVMTEGFLLGWYKNFPM
                     ALLVFAMITAVYLLTFLFSYITRTSTAKSINESLKKFNADEDYQDE"
     gene            complement(4923..5363)
                     /locus_tag="PLS64_07205"
     CDS             complement(4923..5363)
                     /locus_tag="PLS64_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRL43645.1"
                     /translation="MFNVTIRQVGIEEREEIIICCHEISDEVMSLANSIKGQKSFLLA
                     SKDGEMFKLDTKEIFYIESVDNKTFIYCKNNIYETKQKLYELEEILKGTKFFRCSKSM
                     ILNIGKIRSVSPSVNSRFEAKLVNGETAIISRQYVPTLKKKLGM"
BASE COUNT         1697 a         1074 c         1136 g         1637 t
ORIGIN      
        1 tgatccgttc tataacgctc ccgttgttct gattgtgctt gccgataaag cagctcctac
       61 atatttgtat gacggtactc ttgtaatgga aaatcttatg cttgccgccc atgatgaggg
      121 actcggaagc tgttggattc accgtgcaaa ggaagaattt gaaagcaaag agggcaaaga
      181 aattctcaaa tcactcggta ttgagggtga ctatgagggt atcggtcact gcgttatcgg
      241 ttatgtgaac ggtgaatatc ccaaaacttc accgagaaga gaaaaccgtg ttttttatgt
      301 tgaatgaggg aatttgtttt tgaaaggatt gaacaaggtt gactttgcaa cagttgaaat
      361 atgttgtcac cgttgcccgc acaggctcaa tgagtgaagc gtcaaaagaa ctttttattt
      421 cccaaccggg acttacaaca tcaatccgtg agttggaaac ggaaatgggc ataaagattt
      481 ttacacgcac aaacaagggc gttgtaactt caaacgaggg tgaaagattt ctcggctatg
      541 cacgacaggt tcttgaacag gcaagcctgc ttgagggagt gtataagtcg ggaacaaaga
      601 taaagcaaca gttctgcgtt tcaacccagc actactcgtt tgcagtcaat gcatttgttg
      661 atcttatccg acggtttgca ggcgaggagt acgatttttc acttcgtgaa accgaaacct
      721 acgaaattat tgacgatgtt gcaaagatga aaagcgaaat cggaattctt tatctcaaca
      781 gctttannnn nnnnnngcgc tgatgaaaat tataaaatca aagtcgttga catttaccga
      841 gctttttacg gcaaagcctc atgtgtttgt atcaaccgac catccgcttg cagacaaaaa
      901 atccgttaca atggaggagc ttgccgatta tccgtatctc tcgtttgaac agggggagca
      961 taattctttc tattattcgg aagaaatttt cagcacggtc gggcgtacaa aaaatatcag
     1021 agtgaccgac agagcaaccc tgttcaacct tttaatcgga ctcaacggct acacagtttg
     1081 cagcggtgta atcgacaatg agcttaacgg tgataatata accgcaatcc cccttgatga
     1141 agagggcaat atgaaaatcg gttatatcga aaataaaaag attctgcaca gtaaccttgc
     1201 cgaggcatat attgaggctt taaagaaaca tatttgatat aagaaaaact aatagcaaac
     1261 ggctgataac aagtatttta caagtctgtg aattcataat ataattaact catcaaattt
     1321 ccacggaggt atttaagatg agtaaacagc acacaccatt tagatttgaa acagtaggca
     1381 gctttttaag acccgattat ctaaaaaaag caagacttga ttttgaaaac ggtaaaatca
     1441 caaaagaaga gcttaccgca gttgaggaca aggctattct tgaccttgtt gcaaaacaga
     1501 aaaaagccgg tataaaagcg attaccgacg gtgaattccg cagagcaaca tggcatcttg
     1561 acttcatgtg gggatttaac ggagttgaac acaaaaaaac cgaaaacggt gttacattcc
     1621 acggcgaaca ggctttgatt gacgatacat ggctttccgg cgaaatctcg gttgacagtc
     1681 atccgtttgt tgaacattac aaatttgtaa aggctcttga ggatgaaaac acagtagcaa
     1741 agcagacaat tcctgctccc gcacagtttt tccagcagtt catcattcct gcaaatattg
     1801 aaacgacaag gaagttctat tcgacagatg aagaacttat caacgatatt gcaaacggat
     1861 acaaaaaggt aatcaaagat ctttacgatg caggttgcag aaatatccag tttgacgact
     1921 gcacatgggg cgttcttgtt gcggagggta gcgtaaaccg ctatggcgag gatgcagatt
     1981 ttaagagtat cagtgagaaa cttctcaagg taaacaacct tgcaattgag ggtaagcccg
     2041 atgacctcgt aatcaatact catgtttgca gaggtaacta tcattctgca tacttcagca
     2101 gcggtgcata tgatcctgtt gcagagaaac ttttcggtga agaaaatgtc aacgcttatt
     2161 atcttgagtt tgacgatgag cgttcaggcg gttttgaacc gttaaaatat gtgagcggtg
     2221 acaaaaaggt tgttctcggt cttatcacaa caaagtcacc tgttcttgag gataagcaga
     2281 ctgtaatcaa tcgtattcat gaggcggcaa agtatattcc gcttgacaga ttgtatctca
     2341 gtccgcagtg cggttttgca tcatgcgaaa tcggtaataa gcttaccgag gaagagcagt
     2401 gggcaaagct tgctcttgta aaagaaattg cagaagaagt ttggaagtaa ctatatataa
     2461 aaaagccgac acttttcgga gtgtcggctt tttatctgtc ataaatcagt ccttgcgttt
     2521 tgaactgaat ttgtatgata taaggttgag gataaggaaa attaagattg caccgattga
     2581 gtacatcatc ataaatgtga aatccacttc gtttccgaaa aggttgaggt tggttgcaaa
     2641 cgctttttct gcctcagcgg caaacattcc gtggtctgct cctgcgagtg aggtcgaaat
     2701 atcgttaatg cacgggctga caagcacatt tcttatcata gccgcaatgt gtgagccggg
     2761 gataagattt acaaatgtct gaactccgtc cgaaaactgg ctgacgggaa tgtatgcgcc
     2821 gattacaaaa ccgattgcaa ccgaaataag tgtgttgaat gcggcatagg ttgaattctt
     2881 tttgaagaat gacgcaacaa acataagaat aagtacagcc gaaacagagc ctaaaatcac
     2941 aagtccgtat aattttaaaa gctctgtaaa ttcgggaaag cttgaacccg atatgcacga
     3001 aaatgcgatg cctgccgtca gcaaaattgc gctgattaca attgtgttta taaccgcacc
     3061 cgaaaagtag gagagaacaa ccgtactgcc ctttacggaa gaagcgttgt agtcgtagtc
     3121 aatcttgttc tgcttgtcgg aaatcattac cgaaagcgag ttcattgcaa ccgtaacaac
     3181 agatgttccg ataacgcccg atacaagcca tgaatttaca agtgcgttaa tcaaattgtc
     3241 ctctgttttg atgttgaagc cgtcaagcat tgaagtcatt gaatcaacat aattctgctt
     3301 gagaaacaaa agatacaatc cgagtatgat aatctgcgtc atcattgaga agataattgc
     3361 ggttttgtcc tttaaatata cctttatatt tcgttttgtc atacccttgt atgagcaaag
     3421 tgttctttga aatttcatat cagtcaccca gccttttacc cgttacggtc agaaacacat
     3481 cgtccatatt gcctttgata atctcaaaat cgacaatttc agtatgtttg tttgtgattt
     3541 caagagcctg tctgctgttt ttgattttga ttttatacgc atctccgact ctttcaaatt
     3601 cgagattatc ggccttgaga agattatcgt tttcagaatt ttcttccgtg taccaaacaa
     3661 ggctgtttga cgaataatct tttttgagat tatgcggaga atcgttggca acaattttgc
     3721 ccgaatcaat aatcaccaca ttgtcgcagt caactgtttc ttccatatag tgggttgtga
     3781 ggaataccgt aagtcctgtt tctttgcgaa gattgtggat gatttcccac acctgcagcc
     3841 ttgttttcgg gtcaagtccc gttgtcggtt cgtcaaggaa caggagctta ggtcggttaa
     3901 taagcgccct tgcaatgtca acccgtctgc gctggccacc gctcaatgtg ccgtattttc
     3961 ttttgagaat ttccgaaagg tcaaaggttt gtgtaaggct ttcaattctt ttggcgattt
     4021 cttttttgtt gagtccgtaa tatgacgctc tcgattcaag attttcttta acggtaagct
     4081 gtttgtcaag tacagaaccc tgaaaaacaa ttccgataag ctccttgatt ttgttctttt
     4141 ctttgtcaag gtcatatccg ccgattttaa cattgcccga tgttttttca agcaccgtac
     4201 aaagaatgtt gattgtggtt gatttgcctg caccgttaat tccgagaaat gcgaaaaacg
     4261 aaccttcttc aaccgtgaat gatatatcat ttactgcgtg aacatcttta taattcttta
     4321 caagattttt aacttcgatt atgttactca ttaaaaatcc cctccttctg tcaacatagt
     4381 agcaccttgc aattcaaaag aaaagcaatt ttagctgtgt tgcaaaaaga ggggagtaag
     4441 ttgcgatttt gtatgtcaaa atgcagttat tcgtcctgat aatcctcgtc tgcgttaaat
     4501 tttttgagcg attcattaat tgattttgcc gtgcttgtcc gtgtaatgta gctgaaaaga
     4561 aatgtcagca gatacacggc ggtaatcatt gcaaaaacca aaagcgccat aggaaaattc
     4621 ttgtaccaac caagcaaaaa tccctcggtc ataacaattg cctgaataag gcagaaatga
     4681 atgacaattc gtatgcgagc ctgcagtttg gtgggttctt ctttaaagta aaaaagcaga
     4741 gtcggaattg aagttaccgc agctgttccc attacaaccc acggaaaatc ggggggatag
     4801 tattcgatta acgcaattga atttgcaagg cttacaacaa aaagcattgc aaccgtgatt
     4861 atgaaaaagt cctttatgac ttcctttata atttctttta aagaatcatt cataacaata
     4921 cctcacattc caagcttttt cttcaaggtc ggcacatact gccttgaaat aattgccgtt
     4981 tcaccgttta caagctttgc ctcaaaacgg ctgttgacag acggtgacac ggaacggatt
     5041 ttaccgatat tcagtatcat cgactttgaa catctgaaaa attttgttcc ttttaaaatt
     5101 tcttcaagct catacagctt ttgtttggtt tcataaatat tgtttttgca gtatataaag
     5161 gttttgttgt caaccgattc tatgtagaaa atttcctttg tgtcaagctt gaacatctcg
     5221 ccgtcttttg atgcaagaag aaaacttttc tgccccttaa tgctgtttgc aagcgacata
     5281 acctcgtcgc tgatttcatg acagcaaatt ataatttctt cccgttcctc aatcccaacc
     5341 tgacggattg taacattaaa catcgcattc cctccgttca tcaaccattt taccatatca
     5401 aaaaggaaat taacagtacc gaaccctaac ttgcaaaatt atgtcggtaa attacaattt
     5461 tttgttgaca aaaaaatttt tttaaatata atagaaattg aataatacta ttattgaaaa
     5521 caaaatatat aaagaggtgt agcaatgaat ttat
//
LOCUS       DAOMVE010000121         5383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_4191_length_5383_cov_3.612237, whole genome shotgun sequence.
ACCESSION   DAOMVE010000121 DAOMVE010000000
VERSION     DAOMVE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4191_length_5383_cov_3.612237"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLS64_07210"
     CDS             complement(<1..205)
                     /locus_tag="PLS64_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL43646.1"
                     /translation="MKLNKNRDLTTGSITGGLWSFAVPLMLGNVFQQFYNLADTWMVG
                     KYVGDNALAAVGSSYDRFFVYGFK"
     gene            complement(242..658)
                     /locus_tag="PLS64_07215"
     CDS             complement(242..658)
                     /locus_tag="PLS64_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43647.1"
                     /translation="MRGLFNRFANFMQGRYGTDKLNNALWVLLLILWFVNIFVWNRVA
                     RYIIDGIMLLIIVLICFRMFSRNIVARSAENRKFLPIYNAVTGWFKLTFKKIRDRKDY
                     RYIKCPVCKAQLRVRNKKGNHTVRCPKCGSEFNKKI"
     gene            complement(768..3167)
                     /locus_tag="PLS64_07220"
     CDS             complement(768..3167)
                     /locus_tag="PLS64_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRL43648.1"
                     /translation="MFKNFSNFSEYLNQKNFILLDGATGTLIQKSGVKYDHVPEVLNI
                     THPELIESFHRRYIDAGSDIVYANTFGANAYKLQDSGYSVEEIIGAGVKIARKAVEGT
                     DALVALDIGPIGQLLEPAGSMKFDEAYEYYKQQILTGKEADVIAFETMTDLYELKAAV
                     LAAKENCDKPILTTMTFERNGRTFTGVSPACAAVTLTGLGVDALGVNCSLGPDELEPV
                     VSEMSKYTNLPLVIKANAGLPDPNSNEYNIMPDKFAECVCSLLKYGVKVIGGCCGTNP
                     DYIAKIKSEVADREYQPQTKSVDTTVCSSTTVVEINGPRIIGERINPTGKKLFKQALV
                     ENNIDYILTQALSQVQGGAEILDVNVGHPEIDEKKMMVRVLKAIQSVCDVPLQIDSTK
                     PEVIEAGLRYYNGKPILNSVNGEEQSLATVLPLVKKYGASVVGLALDENGIPKTAEGR
                     FEIAKRIVERAEAVGIDRKDVYIDCLTLTVSAEQAACRQTLEALHRVKTELGCKTCLG
                     VSNISFGLPNRELVNRTFLTMAMEQGLDLPIINPNVESMTGAVRAFRVLNGIDKNSLE
                     FINAYNDAVPAAPVKKADSNVDIFAAVYNGLKNEGAAATAKLLETTDAMQVVNEMLIP
                     ALDRVGEDFEKNKIFLPQLIQSANTAQECFDVIKKHLTKTSGTPVSKGKIIVATVKGD
                     IHDIGKNIVKVLLDNYGYTVVDLGRDVDPQLIVDTAVEQNITMIGLSALMTTTLKSME
                     DTIRLVRECKELQNPDGSSKCTIFVGGAVLTKDYAMKIGADYYCRDAKESVDTAKLVL
                     G"
     gene            complement(3160..3804)
                     /locus_tag="PLS64_07225"
     CDS             complement(3160..3804)
                     /locus_tag="PLS64_07225"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRL43649.1"
                     /translation="MITLDRLNRKEAIRYLGGAGISLNYRMDVLMDECEKEILKTAEP
                     RFLYVERKAPFDDILLGNDIKKHLEGCHTAVMMCATIGSEIDKLIRISQISDMARAVV
                     LDSFASVAVEQVCQKVDEILAEKYSGKYMTFRFSPGYGDYPIEMQKEYLRILDAPRKI
                     GLTTGDSCLLVPSKSVTAVLGISDNPIERRKRGCAICSMKGKCRFRRNGEHCGV"
     gene            complement(3801..4661)
                     /gene="metF"
                     /locus_tag="PLS64_07230"
     CDS             complement(3801..4661)
                     /gene="metF"
                     /locus_tag="PLS64_07230"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRL43650.1"
                     /translation="MKISNLFNSGKTVFSFEVFPPKKTSPIESVYGKLEEISALKPDF
                     ISVTYSAGGSGSKSRTCEIATKIKRDFGVESVAHLTCINSTKADIDRNLALFKENGIE
                     NILALRGDRVEGVEPQKDFTYASDLCRYIASQGDFDIAGACYPEVHSEATDEVEDIHN
                     LRKKVESGASHLISQLFFDNSVYYRFLERAKIAGVNVPIEAGIMPVTSKSQIERMVSM
                     CGASLPAKFAKIMQKYDTRPEALRDAGIAYAVDQIVDLIAHGVDGIHLYTMNNPYVAR
                     KITEAVSSLL"
     assembly_gap    4835..4844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    5083..5092
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1436 a         1240 c         1107 g         1580 t
ORIGIN      
        1 gcttaaatcc gtacacaaaa aatctatcat atgacgagcc gactgccgca agggcattgt
       61 cgcccacata ttttccgacc atccatgtat cggcaaggtt gtaaaactgc tgaaacacat
      121 tgccgagcat aagcggtact gcaaaagacc acaggccgcc tgttatactg cctgtggtca
      181 aatctctgtt tttatttagt tttatcacaa aatcatttcg ttgcaaccgt agattctgcg
      241 gtcatatttt tttgttaaat tcacttccgc attttggaca gcgaacagta tgattgcctt
      301 ttttgttgcg aacacgaagc tgcgccttgc acacgggaca cttgatataa cggtagtcct
      361 tgcggtcacg aattttcttg aaggtcagtt tgaaccaacc tgttaccgca ttgtaaatcg
      421 gaagaaattt tctgttttcg gcacttcttg caacaatgtt gcgtgagaac atacgaaaac
      481 agataagcac gataatcaag agcattattc cgtcaatgat gtatcttgca acacggttcc
      541 acacaaaaat attcacgaac cacagtataa gcaacagcac ccagagtgca ttgttgagct
      601 tatccgtgcc gtatctgccc tgcataaaat tggcgaatct gttaaataat cctcgcataa
      661 agttcatcct ttccgcaaaa agcggtaatt acaaaaatca tccttccacc attttatcac
      721 aggcagggcg tacattcaat aaacatttga cgatatatgt cactaaatca gccgagaacg
      781 agctttgccg tgtcaacgct ttcttttgcg tcacggcagt agtagtcagc gccgattttc
      841 atagcgtagt cctttgtaag aaccgcaccg ccgacaaata ttgtgcattt gcttgagccg
      901 tcgggattct gaagttcctt acattctctt acaagcctta tcgtgtcttc catactcttg
      961 agggttgttg tcataagagc cgaaagtcct atcattgtaa tattctgttc aacggcagta
     1021 tcaacaataa gctgaggatc aacatcacga ccgaggtcaa caactgtgta gccgtagtta
     1081 tcaagaagaa cctttacaat gttcttgccg atatcgtgaa tgtcgccctt tacggttgcc
     1141 acaataattt ttcccttgct tacgggagtt ccgcttgtct ttgtaaggtg ctttttaatt
     1201 acatcaaagc actcctgagc tgtgtttgca ctttggataa gctgtggaag gaagatttta
     1261 ttcttttcaa aatcttcacc cactctgtca agtgcaggaa taagcatttc gtttacaacc
     1321 tgcattgcat cggttgtttc aagaagcttt gcggttgcgg ctgcaccttc gtttttaagt
     1381 ccgttataaa cagccgcaaa aatatcaaca ttgctgtctg cctttttaac gggggcggca
     1441 ggaacagcat cgttatatgc gttgataaat tcaagcgagt tcttgtcaat gccgttaagc
     1501 acacggaatg ctcttacagc gcctgtcatc gactcaacat tcgggttgat aatcggcaaa
     1561 tcaagtccct gttccatagc cattgtaagg aaagttctgt tgacaagctc acggttcgga
     1621 agtccgaacg aaatgtttga tacaccgaga caggttttac agccaagttc cgtttttact
     1681 ctgtgcaagg cctcaagagt ctgacggcaa gccgcctgtt ctgcggaaac cgtaagagta
     1741 agacagtcga tatatacatc ttttctgtca atgccgacag cctcggctct ttcaacaatt
     1801 cgttttgcaa tctcaaatct gccctcggcg gttttcggga taccgttttc gtcaagtgca
     1861 agtccgacaa cagacgcacc gtactttttg acaagaggaa gaaccgttgc aaggctctgt
     1921 tcctcaccgt ttacggagtt cagaatcggt ttgccgttgt agtagcggag tccggcctca
     1981 ataacctcgg gctttgtgga gtcaatctgg agcggaacat cacacacact ctgaatagcc
     2041 ttgagaacac gcaccatcat ctttttttcg tcaatttcgg gatggcctac attgacatca
     2101 agaatttccg caccgccctg aacctgactg agagcctgag tgaggatata gtcaatatta
     2161 ttttcaacaa gcgcctgctt gaacagcttt ttgcctgtgg ggttaattct ttcgccgata
     2221 attctcggac cgttgatttc aacaaccgtc gttgaactgc aaacggttgt gtcaacgctc
     2281 tttgtctgag gctgatattc tctgtcggca acctccgact taatttttgc aatataatcg
     2341 ggattcgttc cgcagcatcc accgataacc ttaacaccgt atttgaggag tgaacaaacg
     2401 cactcggcaa atttatcggg cataatgttg tactcgttgc tgttaggatc gggcaaacct
     2461 gcgttagcct tgattacaag gggaagattt gtgtatttcg acatctcgga aacaacgggt
     2521 tcaagctcat ccggaccgag tgaacagttt acaccgagag cgtcaacacc gagtcccgta
     2581 agagtgacag ccgcacaggc aggggataca cccgtaaatg ttcgtccgtt tctctcaaag
     2641 gtcattgttg tgaggatagg tttgtcgcag ttttcttttg cggcaaggac tgcggcttta
     2701 agctcgtaaa ggtcggtcat tgtttcaaaa gctatgacat cggcctcttt accggtaaga
     2761 atctgctgtt tgtaatactc ataagcctcg tcaaatttca ttgaaccggc aggttcaagc
     2821 aactgaccta tcggaccgat atcaagggca acgagggcgt ctgtaccctc aacggctttt
     2881 cttgcaatct taacaccggc accgatgatt tcttcaacgc tgtaaccgct gtcctgaagc
     2941 ttgtaggcat ttgcaccgaa ggtgtttgcg tacacaatgt cagatccggc atcaatatac
     3001 ctcctgtgaa agctctcgat aagctcgggg tgagtaatgt tcagcacctc aggcacatgg
     3061 tcgtacttta caccgctttt ctgaatgaga gttcctgttg caccgtcaag cagaataaaa
     3121 ttcttttggt tgagatattc actaaagttt gaaaaattct taaacaccac aatgttcccc
     3181 atttcttctg aagcgacatt tgcctttcat actgcaaatt gcacaccctc gttttcttcg
     3241 ttcaatcgga ttgtctgaaa ttccgagaac cgccgtaacc gactttgacg gcacaagcag
     3301 acagctgtcg cctgttgtaa gacctatttt tctcggagcg tcaagtattc tcagatactc
     3361 cttctgcatc tcaataggat agtccccata gccggggctg aaacggaagg tcatatactt
     3421 tccgctgtat ttttcggcaa ggatttcgtc aaccttttga cacacctgtt caacggcaac
     3481 gcttgcaaag ctgtcaagca caaccgccct tgccatatcg gaaatctgac ttatacggat
     3541 aagcttgtca atttccgaac cgattgtcgc acacatcata acggctgtgt ggcaaccctc
     3601 aaggtgcttt tttatatcat tgccgagtaa aatatcgtca aacggagctt tgcgttcaac
     3661 atagagaaat ctcggctctg ctgttttcag tatttctttt tcgcattcat ccatcagcac
     3721 atccatgcgg taattaaggc tgattcctgc accgccgagg tagcgtatag cctctttgcg
     3781 gtttaatctg tcaagcgtta tcataaaaga cttgacaccg cctccgtaat ttttcttgca
     3841 acatacgggt tgttcattgt gtagaggtgg atgccgtcaa caccgtgtgc gataaggtca
     3901 acaatctggt caaccgcata tgcaatgcct gcgtcacgca aagcctcggg ccttgtatca
     3961 tacttctgca taatctttgc aaattttgca ggaaggcttg caccgcacat tgaaaccatt
     4021 ctttcaatct gacttttgct tgttacaggc ataatacctg cctcaatcgg aacattcacg
     4081 cctgcaatct tcgctctttc aagaaaacga tagtaaacgg agttgtcaaa gaaaagctgt
     4141 gaaataaggt ggcttgcacc cgactcaacc tttttgcgaa ggttgtgaat gtcctcaacc
     4201 tcatcggttg cctcgctgtg aacctcggga taacaagcgc cggcaatgtc aaaatcaccc
     4261 tgtgacgcaa tatatctgca caaatcgctt gcataagtga aatccttctg cggttcaaca
     4321 ccttcaaccc tgtcaccacg gagtgcaaga atattctcaa tgccgttctc tttaaaaagc
     4381 gcaaggttgc ggtcaatatc agcctttgtg ctgttaatgc aggtgaggtg tgccacggat
     4441 tcaactccga aatctctctt gatttttgtt gcgatttcgc aggtacggct ctttgaaccg
     4501 ctgccgcctg cgctgtatgt tacgctgatg aagtcaggct ttaaagccga aatttcttca
     4561 agcttgccgt aaaccgattc aatcggagag gtctttttag gcggaaatac ctcaaaagaa
     4621 aaaactgttt ttccgctgtt aaataaattg cttattttca tatataaaca cctcggtaat
     4681 ggttttgtta aacttgatta attatagcat aaataatttt tgcatacaat actgttttgg
     4741 tgggttttgt cgtttaaggc tatcattttt tgggagattt ggatataaat gaaagttatg
     4801 acaaggagtt ttgttatatg tagcacggtg taggnnnnnn nnnnattata attttgttaa
     4861 cagaccaaat tcaccattat actgtagggg cgttcattgg acgcccgcaa gtaacgacac
     4921 aatttttttg ataaaaataa accatataaa tgagatacaa ttacctaaat gatgtagggg
     4981 agaactgtgt tctcccgtta gcaatggtac aaatttgatg tcaaatcaca ttatataaaa
     5041 atcacaaagt tactgatatt ttgtagcggc gaacctcgtt cgnnnnnnnn nnaaccaaaa
     5101 cgaacacaca atttttgtag tatgaaatga tatggtagct gacaatagtc tgtattaagt
     5161 cgaaccgatt ttacaatcgt ttcgattggc gaacacggtt cgccgctaca gactatacac
     5221 cgttttcatc aaatcaaaaa tttatgttct aacaaattat ttcatcaagg cgggcgtcca
     5281 atgaacgccc ctactgatta aacaaaaacg gtcgggaaac aatgatttgc attttgcaaa
     5341 ctgtcttccc gacctcaatt ttcaattttc cattttcaac ttt
//
LOCUS       DAOMVE010000122         5370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_4210_length_5370_cov_3.127375, whole genome shotgun sequence.
ACCESSION   DAOMVE010000122 DAOMVE010000000
VERSION     DAOMVE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4210_length_5370_cov_3.127375"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLS64_07235"
     CDS             <1..301
                     /locus_tag="PLS64_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43651.1"
                     /translation="GYYVIMYYVGLNSKEESLARIENRVRKGGHDIPKNDVCRRFDNR
                     FDSLKRIVPLCDEVIFYDNENGFIKVAEIKNNKFNYSNGYRPKWITEFENKLKLG"
     gene            complement(389..1447)
                     /locus_tag="PLS64_07240"
     CDS             complement(389..1447)
                     /locus_tag="PLS64_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43652.1"
                     /translation="MYVELKNINKTFGDYKASDNVNFGIEKGKLIGLLGPSGSGKTTI
                     LRMIAGLENPDSGEIVIDGKVVNDIPASKRGIGFVFQNYALFRYMTVFDNIAFGLKIQ
                     KKSKKFIKERVNELVKLIGLEGLEKRYPSQLSGGQKQRVAFARALAPNPNLLLLDEPF
                     AAIDAKVRQELRSWLKDMIERLGVTSIFVTHDQSEAIEVADEIIITNKGRIEQIGSPI
                     DIYQHPQTAFSASFFGQTTTLDDYTKFKTFDVVDNVDRAVIRPEFVKVTKLSETTAQK
                     ASVSVGEVIKTSFRGDNIEIAVKCDGGTVYAYRGLDETPITVGEKVNVFLYRLFITKG
                     DEVQILRNKSLQEESVII"
     gene            complement(1447..2268)
                     /gene="cysW"
                     /locus_tag="PLS64_07245"
     CDS             complement(1447..2268)
                     /gene="cysW"
                     /locus_tag="PLS64_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HRL43653.1"
                     /translation="MYEETKRSKVVKYILIGIALLFVFVMLVLPLVTVICEAFKSGAE
                     VFWQAVSDDYTVKAIVLTVEATVFAVLFNTVFGIFAAWSITKFRFKGKKLLTTLIDLP
                     VTVSPIIAGLIFVLTFGRQSPIYPLLSELGIKVIFAVPGIILATVFVTFPFISRELIP
                     VLESEGTDEEEAAALMGAKGTTIFRKITFPHIKWAFLYGVVLCAARAMGEFGAVSVIS
                     GHLRGKTNTLPLHVEILFNEFKYVPAFAVSSILVLMAIAILIIRSVVEYKGKKDA"
     gene            complement(2270..3121)
                     /gene="cysT"
                     /locus_tag="PLS64_07250"
     CDS             complement(2270..3121)
                     /gene="cysT"
                     /locus_tag="PLS64_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HRL43654.1"
                     /translation="MMVQAKKSKKAKRVIPGFGLSLGVTITLLSLVVLIPLAALVIYT
                     AQMSPSEFWEAITRERVLASYKVSFITAFIASLINAVMGVILAWVLVRYKFPGKRILD
                     GMIELPFALPTAVAGIALTSLTADTGLIGGFFAKFGISIAYTRVGITVALVFVGIPFV
                     TRAVQPVLEKLDPVYEEAAGVMGAGRFRIFRKIILPEILPSVLTGFGLAFGRCLGEYG
                     SVVFIAGNKPFETEITPLIIMSELQEYDYKSATAIALVMLAASFLILFLVNMVQHRNA
                     KILKGGR"
     gene            complement(3204..>3775)
                     /locus_tag="PLS64_07255"
     CDS             complement(3204..>3775)
                     /locus_tag="PLS64_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL43655.1"
                     /translation="VITPNPKTSGGARWNYLAAWAYADKKYNGDEAKIEDFIKKLYQN
                     VLVLDSGARGATTSFVENGQGDVLLAWENEAYLSIKDNPDEYEIVTPSVSILAQPSVA
                     VVDEVVDQRGTRKVATEYLSYLYSDEAQRIAGDNYYRPYNKEILKEYSDVFDLNINLV
                     TINDFGGWDEAQKKHFADGGIFDKIYEKK"
     assembly_gap    3776..3785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3786..4254)
                     /locus_tag="PLS64_07260"
     CDS             complement(<3786..4254)
                     /locus_tag="PLS64_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRL43656.1"
                     /translation="MKNLRTKVLSAVLAVSVLGASAVAFTGCGKKDTTNDSKVTLTNV
                     SYDPTRELYESYNKIFAKHWKEETGQDVEVTQSHGGSGKQALEVANGLEADVVTLALE
                     YDVNAIRDAGLIEDGWVNEFDNDSSPYTSTIVFLVRKGNPKNIKDWDDVAKDGV"
     gene            4788..5333
                     /locus_tag="PLS64_07265"
     CDS             4788..5333
                     /locus_tag="PLS64_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRL43657.1"
                     /translation="MAKTTKYSGTQTEKNLEAAFAGESQARNKYTYFASVAKKQGFEQ
                     IANLFLKTADNEKEHAKMWFKELNGIGDTAENLLAAAEGENYEWTDMYEDFAKTAEEE
                     GFTELAHKFRLVAAIEKHHEERYRALLRNVETAEVFKRSEIKVWECRNCGHIVVGKEA
                     PEVCPVCNHPQSYFEIHAENY"
BASE COUNT         1617 a         1149 c         1036 g         1558 t
ORIGIN      
        1 gggctactat gttattatgt actatgtagg tcttaactca aaagaagaaa gtcttgcaag
       61 aattgaaaac agggtacgga aaggcggtca tgatattccg aaaaatgatg tgtgcagaag
      121 atttgataac cgcttcgatt ctttaaaaag aattgttccg ctgtgtgatg aagtaatttt
      181 ttatgataat gaaaacggat ttattaaagt tgccgaaata aaaaacaaca aattcaatta
      241 ttcaaacggt tacagaccta aatggattac agaatttgaa aataaactga aattaggcta
      301 aggaaatgca aagcaccgag agaaattacc ctcggtgctt tgcttgtttt tataggattt
      361 tttacagttg atatcgtgat taaacagatt agatgataac cgattcttcc tgaagtgatt
      421 tgttacggag aatctgaacc tcatcgcctt tggtaatgaa aagccgataa agaaatacat
      481 tgaccttttc cccgacggta atcggagttt cgtcaagtcc tctgtacgca taaaccgtgc
      541 ctccgtcaca ttttacggca atttcgatat tgtcgcctct gaacgaagtc tttataacct
      601 cgccgacgga tacagacgct ttttgtgcgg ttgtttcgct gagctttgtc acctttacaa
      661 attcgggacg gataactgca cggtcaacat tatcgacaac atcaaaggtc ttgaactttg
      721 tataatcgtc aagcgtagta gtctgaccga aaaatgatgc ggaaaatgcg gtttggggat
      781 gctgataaat gtcaatcggc gagcctatct gctcaattct gcccttgttt gtaattataa
      841 tttcatcggc gacttcaatc gcctcgctct gatcgtgggt tacaaatata cttgtaacgc
      901 cgagtctttc aatcatatct ttaagccatg agcgaagctc ctgcctaacc tttgcgtcaa
      961 ttgcggcaaa tggttcgtca agcagaagaa gattcgggtt tggcgcaagc gctcttgcaa
     1021 aggcaacacg ctgtttttga ccgcccgaaa gctggcttgg ataacgcttt tcaagtcctt
     1081 caagaccgat aagctttaca agctcgttta ctctttcctt gataaatttc ttgctctttt
     1141 tctgaatttt cagaccgaaa gcaatgttat caaaaactgt catatatctg aaaagagcgt
     1201 agttttggaa tacaaagcct atgcctcttt tgcttgcggg aatatcgttg acaacctttc
     1261 cgtcaattac aatttcgccg ctgtcgggat tttcaagtcc tgcaatcata cgaagaattg
     1321 ttgttttgcc gctgcccgaa ggaccgagaa gtccgataag cttacccttt tcaataccaa
     1381 agtttacatt gtcggatgcc ttgtaatcac cgaaagtttt atttatattt ttaagctcaa
     1441 catacattac gcatccttct ttcctttgta ttcaaccaca cttctgataa tcaaaatcgc
     1501 aatcgccata agcacaagta ttgatgaaac cgcaaatgcg ggaacatact tgaactcgtt
     1561 gaacaaaatt tcaacatgga gaggaagtgt gtttgttttt cctcttaaat gtcccgaaat
     1621 taccgaaact gcaccaaatt cacccattgc tcttgcggca cagagtacaa ctccgtaaag
     1681 gaatgcccat tttatgtgcg gaaatgttat ttttctgaaa attgttgtgc cttttgcacc
     1741 cataagtgcg gcggcttctt cctcatccgt accctcggat tcaagcacgg gaataagctc
     1801 ccttgagatg aacgggaatg ttacaaacac ggttgcaagg ataattcccg gaacggcaaa
     1861 gataaccttg attccaagtt cgcttaaaag cggatatatc gggctttgtc tgccgaatgt
     1921 gagtacaaat ataagacctg cgattatggg cgaaaccgtt acggggaggt cgatgagtgt
     1981 tgtaagaagt tttttgccct tgaatctgaa ctttgtaatt gaccaggcgg caaaaattcc
     2041 gaataccgta ttgaacagta ccgcaaacac ggttgcctca acagtaagca caattgcctt
     2101 taccgtgtaa tcatcgctga cagcctgcca gaacacctct gcaccgcttt tgaaagcctc
     2161 gcagattacc gtaacaagag gaagaacgag cattacgaat acaaataaaa gtgcaatgcc
     2221 tatgagtatg tactttacaa cctttgaacg ctttgtttct tcgtacattt tatcttcccc
     2281 ctttcagaat ttttgcattt ctgtgctgaa ccatatttac aaggaacaga atcaaaaaag
     2341 atgcggcaag cattacaagt gcgattgccg ttgcactttt gtagtcatat tcctgcagct
     2401 ccgacataat gataagcgga gtgatttctg tttcaaacgg cttgttgcct gcaataaaaa
     2461 caaccgaacc gtactcacca agacatcttc cgaatgcaag accaaagcct gtgagaaccg
     2521 agggaagaat ttcgggaaga ataatttttc tgaaaattct gaatctgccg gcacccataa
     2581 ctcctgccgc ctcttcataa acaggatcaa gcttttcaag aacaggctga actgctcttg
     2641 ttacaaaagg tattcccaca aatacaagtg caaccgtaat accaactctt gtgtaggcaa
     2701 tgcttatgcc gaactttgca aaaaatccac cgataagacc tgtgtcggca gtcagagaag
     2761 taagtgcaat gcctgcaacc gctgtcggaa gtgcaaacgg aagctcaatc ataccgtcaa
     2821 gaattctttt gccggggaat ttgtaacgaa caagcaccca cgcaagaatt acacccataa
     2881 cggcattgat gagagatgca ataaatgcgg ttataaaact aaccttgtag cttgcaagca
     2941 ctctttcacg ggtgattgcc tcccaaaatt cagaggggct catctgtgcc gtataaataa
     3001 cgagagcggc aagaggaatc agcacaacaa ggcttaataa tgtaattgtt acacccaaag
     3061 aaagaccgaa gcccgggatt actctttttg ctttttttga cttttttgcc tgaaccatca
     3121 tcacacctgt ttcccctgta aaatatcatc cgacatcaga cagaattgcc tataaactgc
     3181 ctgatgtcat tttttaatcg gttttatttt ttctcgtaaa tcttatcgaa gataccgccg
     3241 tctgcaaagt gctttttctg agcctcgtcc caaccgccga aatcgttgat tgttacaaga
     3301 ttaatgttga ggtcgaacac atcggagtat tccttcaaaa tttccttatt ataagggcgg
     3361 taatagttat cgcctgcaat acgctgagcc tcatcgctgt aaagataact taaatactct
     3421 gttgcaacct ttcgagtgcc tctttggtca accacttcat caacaacagc aacagacggc
     3481 tgtgcaagaa tgctgacgct cggagttacg atttcgtact cgtcgggatt gtccttgatt
     3541 gaaagatacg cctcattctc ccaagcaaga agaacatcgc cctgtccgtt ttctacaaat
     3601 gaagttgtag cacctcttgc gcctgagtca agcacaagca cattttgata aagcttttta
     3661 atgaaatctt cgatttttgc ctcatcaccg ttatatttct tgtctgcata tgcccaagcg
     3721 gcaaggtagt tccatcttgc acctcccgaa gttttcggat ttggagtgat aacgcnnnnn
     3781 nnnnnccaca ccgtcttttg caacatcgtc ccaatccttg atgtttttcg gattaccttt
     3841 gcgaacaagg aatacaattg ttgaagtgta tggtgaagag tcattgtcaa actcatttac
     3901 ccaaccgtcc tcaatcagac ctgcgtcacg aattgcgttt acatcgtatt caagtgcgag
     3961 tgttacaaca tccgcctcaa gaccgtttgc aacctcaaga gcctgtttgc ccgaaccgcc
     4021 gtgtgactgg gtaacttcaa catcctgtcc tgtttcttct ttccaatgct ttgcaaagat
     4081 tttgttatat gattcataaa gctctcgtgt agggtcatac gaaacatttg ttaatgttac
     4141 ttttgaatcg tttgttgtat ctttttttcc gcatcctgta aaggcaaccg ctgacgcacc
     4201 caatactgaa actgccaata ctgccgataa tacttttgtt cttaaatttt tcattttgat
     4261 tacctcactt taaattaatt tgattttaga ttctgacagg caattacata caacagtacg
     4321 gcttgcctcc aaatgaggta cacattcgta ttttcttcag attgtaaatg tttttcataa
     4381 gcgtcacctt ttggattttt gaggttattt tgtttaacct acaattccta tcaatttact
     4441 atgcgtttct ttgagaacta ttttatactg tacagggtga aaagtcaagg taaaattttt
     4501 acacatccaa cactttagca aattgcaaca atacacaagc aatatttttc gcaaaaatag
     4561 taataaaatc gaaaaaacag gcataaaata acggataggt attttcaaat gaaatatgcc
     4621 tatcctaaaa aagaaatatt tttttgcgta aatctattga caaatctata taaaaaaggt
     4681 atagtagtat taaatcaata atgattatta ttttattcaa aaattaaata cacaaaggcg
     4741 tattgagtat tttaaatttc aaaaattaat ttaacggagg aaatattatg gctaaaacaa
     4801 caaagtattc gggaacacag acagaaaaga accttgaggc tgcttttgca ggcgaatcgc
     4861 aggctcgtaa caaatacaca tattttgcat ctgtcgccaa aaagcagggc tttgagcaga
     4921 ttgcaaacct tttcttaaaa acggcagaca acgagaaaga acacgcaaaa atgtggttca
     4981 aggagcttaa cggaatcggc gacacagccg aaaatcttct tgccgctgcc gagggcgaaa
     5041 actacgagtg gacagatatg tacgaagatt tcgctaaaac tgccgaggaa gaaggcttta
     5101 cagagcttgc acacaagttc cgccttgttg ccgcaattga aaagcaccac gaagaaagat
     5161 accgtgcatt actccgcaat gttgaaactg cagaagtttt caaaaggagc gaaatcaagg
     5221 tttgggaatg ccgtaactgc ggtcacattg ttgtaggcaa agaggctccc gaggtttgcc
     5281 ccgtctgcaa ccacccacag agctattttg aaattcacgc agaaaactac taattctgat
     5341 ttaatcaaat atataaagga aactccaccg
//
LOCUS       DAOMVE010000123         5363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_4216_length_5363_cov_3.828937, whole genome shotgun sequence.
ACCESSION   DAOMVE010000123 DAOMVE010000000
VERSION     DAOMVE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4216_length_5363_cov_3.828937"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..244)
                     /gene="rrf"
                     /locus_tag="PLS64_07270"
     rRNA            complement(128..244)
                     /gene="rrf"
                     /locus_tag="PLS64_07270"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            549..1784
                     /locus_tag="PLS64_07275"
     CDS             549..1784
                     /locus_tag="PLS64_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL43658.1"
                     /translation="MNNVNKCKMLLSSLSVFRGIMNRSVPKAFYELLLSLDKKPEEFL
                     GAYGKLCSMLCERGCADKFAFAISETALFDDNCFARAATAGRQGELPENVVSAVKTDC
                     DAILTAAKLTSDEVLEAYTYADEIKELIPILPKWQTGKCAPCFDGFDGSLDKLSAYYK
                     ENGCGMFARYKAFIWRDGDIQPVEHPDRIDMDTFTGYERQRSQVVNNTLSFIQGKSCN
                     NCLLYGDKGTGKSSTVKAIANEFRKDGLRIVEMPKDRLIDFPLLVDKIAALPLKFIIF
                     IDDLSFQHQDQSYTTLKAVLEGGLAARPDNALIYATSNRRHLVKESISDRTHTVDDIN
                     VRDNMQESLSLSDRFGLAVCYTIPSKNDFLEIVYSLAEQRGVKMTHEELALGAEQFAL
                     SRGGRSPRCARQYVESLFC"
     gene            1802..3064
                     /locus_tag="PLS64_07280"
     CDS             1802..3064
                     /locus_tag="PLS64_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRL43659.1"
                     /translation="MKTKIISIALAVVTMLSAVALFSGCSDGEYPVEVANITIESQPK
                     NIVVLDAPTADILIATGYDRYIVGRSDEVDQQSIAVAPSVGSYENPDTDKIINSGTNL
                     VFAGQSINEDAKKKLQDKGIQVVTMSHGDTPKQVETNYVTIGKICGGTKTGAKKGEDT
                     YNELLDKMNNYKQQVNNRNSGILDTVCYLYTEDDRLKMMTSGTYGDMLLGYTGAVNAA
                     VNISDNNVDVATLKIANPNFIFYADKATLDKIKGDTTLASLGAVKTGKILEVSLEEMS
                     RPGNTALEALEKMVYFMYPDLKPKSATDTTKAQDATASKTDATTATQATTTASDSKSV
                     ADKYNLTITSDLSLKQEDENDTVKAMQLRLYNIGYIADKDNVTGYYGEVTAEAVKKFQ
                     KNNGLKETGTADNATLVLLFDSSAKKAK"
     assembly_gap    3199..3208
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3504..4337)
                     /locus_tag="PLS64_07285"
     CDS             complement(3504..4337)
                     /locus_tag="PLS64_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HRL43660.1"
                     /translation="MECIELDNKIKITDVHDLDLAQTLDCGQSFRWKPQADGSFHGVA
                     YGKSVTVSLDKTDMYIENATADDFKNIWYSYFDFSLDYGKIREEISTIHPVLNEAAKY
                     APGIRILRQEPFEALCTFIISQNNNIKRIKGIVERLCENFGTRLDDGEFAFPTAETLA
                     KLSPDDLAPLRAGFRNRYIIDAAQKVANGEVNLDSCFTLGYEDARAELMKITGVGKKV
                     ADCTLLFGMHRIEAFPIDVWMKRAMEKLFPNMNGDDFGQYAGIAQQYIFHYSRMHPEL
                     F"
     gene            4610..5299
                     /locus_tag="PLS64_07290"
     CDS             4610..5299
                     /locus_tag="PLS64_07290"
                     /inference="COORDINATES: protein motif:HMM:NF039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HRL43661.1"
                     /translation="MKETICTIPINDIFMPKCGCPFCRMESMLEEQYVKFITGDAMME
                     PNIRIETNKKGFCHRHFSQMFEKGQKLPNALILESHLQEIIDNDMPKKLKGKPDKKQL
                     EVIKKRLDSCYVCDRIEWDMHHFCDTIFVEWAKGEEFYKLYNEQPFICFKHYDFIMNV
                     ATQKSKLPSKLLPQFYAETAGLTKKYLLSLKEDITYFCSMFDYRNHGKDWGSSKDSIE
                     RSIEFLTGEKP"
BASE COUNT         1622 a         1099 c         1172 g         1460 t
ORIGIN      
        1 ttaaacatat agatactcca attagtgtat atggcattgt aattgttttc gcaatgaacg
       61 cccctacggg tttgcaaagc aaaaaaagta taaaaaaacg cccaccctac tgggtggaca
      121 ctttatatgt tggcatctcc ctatttttcc gggccgtcac cagccaagta tcttcggcac
      181 cactgagctt aacttctgtg ttcggtatgg gaacaggtgg accctcagcg tcatcaacac
      241 caacttatta acttttgttt ctctcttgag aacagtattg attatactac gggctgcacc
      301 aaatgtcaac acttttttta aaaaaattta aaaattcttt taaaaccctg aaaagcccgt
      361 atttatcccg atttatcgcc gttttaaccg tcattgaacc cgatatatac ttgatgcggt
      421 gaaaaattat acatattttg gcttgttaaa gcgtaaaaaa taggatgccg ttgtttgccg
      481 taaattattg actaaacgga taaaaatgtg tacaattaaa tagattagaa cagattggga
      541 tgtttagaat gaataatgta aataagtgta aaatgctttt gtcatcactc tctgtattca
      601 gaggtataat gaacagaagc gttccgaaag ccttttatga acttttgctt tcgctcgaca
      661 aaaagcctga ggaatttctc ggtgcatacg gcaagctttg ctcaatgctt tgcgaaagag
      721 gctgtgcgga taagttcgca tttgcaatca gcgaaacggc tctttttgac gataactgtt
      781 ttgcccgtgc cgcaacagcc ggcagacagg gcgagttacc cgaaaatgtt gtttctgccg
      841 ttaagacaga ctgcgacgct attttaacag ccgcaaagct tacttccgat gaagtgcttg
      901 aggcatatac ctatgccgat gaaatcaagg agcttattcc gattcttcca aagtggcaga
      961 caggcaagtg cgctccgtgc tttgacggat ttgacggttc acttgacaag ctttctgctt
     1021 attataagga aaacggttgc ggaatgtttg cccgttacaa ggcgttcatc tggcgtgacg
     1081 gtgacattca gcctgttgag catccggaca ggattgatat ggatacattt acgggatatg
     1141 agcgtcagag aagtcaggtt gtaaacaaca cactttcatt tattcagggc aagagctgta
     1201 acaactgtct gctttacggt gataagggta caggtaaaag ctcaacagtc aaggcaattg
     1261 caaacgagtt ccgcaaagac ggactccgca ttgttgaaat gcctaaggac aggctcattg
     1321 actttccgct tcttgttgac aagattgccg ctcttccgct taaattcatt atatttattg
     1381 atgatctttc gttccagcat caggatcaga gctatacaac ccttaaagcc gtgcttgagg
     1441 gcggacttgc ggctcgtccc gataatgcgt tgatttatgc aacatcaaat cgcagacacc
     1501 ttgttaagga aagcatttcc gacagaacgc atactgttga tgatatcaat gttcgtgaca
     1561 atatgcagga aagtctttcg ctttcggacc gtttcggtct tgcagtctgc tacacaattc
     1621 cttcaaagaa tgattttctt gaaattgtat attctcttgc agagcagaga ggcgtcaaga
     1681 tgactcacga agagcttgca ctcggcgctg agcagtttgc actttcaaga ggcggtcgtt
     1741 cgccaagatg tgcaagacag tatgttgaat cgctgttttg ctgattaatc ggaggtcagt
     1801 tatgaaaaca aaaataattt caattgctct tgcagttgtg acaatgcttt cggcagttgc
     1861 gcttttcagc ggttgttctg acggagaata tcctgttgag gtggcaaaca tcacgataga
     1921 aagtcagcca aagaacatag ttgttcttga tgcgccgaca gccgatatcc tcatcgcaac
     1981 aggctatgac agatatattg tgggcagaag tgatgaggtt gaccagcagt caatcgcagt
     2041 tgccccgagc gtcggttcat atgaaaatcc cgataccgac aaaattatca attccggcac
     2101 aaaccttgtg tttgcaggtc agtcaataaa tgaggacgca aagaagaagc ttcaggacaa
     2161 gggtattcag gttgtcacaa tgtcacacgg tgacactccc aagcaggttg aaacgaacta
     2221 tgtgacaatc ggcaaaattt gcggcggcac aaaaacgggc gcaaagaagg gtgaagatac
     2281 ctacaatgaa ttgcttgata agatgaacaa ctacaaacag caggttaata acagaaattc
     2341 cggcattctt gatacggttt gttatctcta tacagaagac gacagactca agatgatgac
     2401 aagcggaaca tacggcgata tgctcctcgg ctatacgggg gcggttaatg ccgcagtaaa
     2461 catcagcgac aacaatgttg atgttgcaac tctcaagatt gcaaatccga actttatctt
     2521 ctatgcagac aaagctactc ttgataaaat taagggcgat acaacactcg caagtcttgg
     2581 cgcagtcaag acaggcaaaa ttcttgaggt ttcgcttgaa gaaatgtcaa gacccggcaa
     2641 tactgctctg gaagcacttg aaaaaatggt ttactttatg tatcccgacc tcaagccaaa
     2701 gtctgcaaca gacacaacaa aggctcagga tgcgacagcg tcaaagactg acgcaacaac
     2761 cgcaactcag gcaacaacaa ccgcaagcga ttcaaagtcg gttgccgata agtataatct
     2821 taccattaca tcagaccttt cactcaagca ggaagatgaa aatgacacgg taaaggctat
     2881 gcagctccgc ctttataaca tcggttacat tgccgacaag gacaatgtga caggctacta
     2941 cggcgaagta acagccgagg ctgttaagaa attccagaaa aacaacggtc ttaaagaaac
     3001 aggtacagcc gataacgcaa ctcttgtatt gctttttgac agttcagcga aaaaagccaa
     3061 ataattcaaa ttgaaatttg aaaattgaaa atggaaaatt aatgttggca gtgtaccacc
     3121 gcccgtaatt tgagaagata gttagataaa cgaaatgcat cagatgcccg cataaggctc
     3181 tgatgcattt tgctttatnn nnnnnnnnac agttcagcga aaaaagcgaa ataaatcaga
     3241 aaattgaaaa tgtaaaatta tagtcgggaa gacagtttgc aaaatgcaaa acattgtctt
     3301 cccgactgtt ttttatatgt gatttaccga tataccgtag cggcgatcag cgatcgccat
     3361 ttgatttggt tgtaaaacca aatcaataat agatgccgca ataaaattgt gacaatgcac
     3421 ggtcgaaccg attttgcaat cgtttcgatt ggcggtcact gaccgccgct acaattaaac
     3481 cttaacatta cggtggtaca ccgttaaaac agttccgggt gcattcggct gtagtgaaaa
     3541 atgtactgct gggcgatgcc tgcgtactga ccgaaatcgt caccgttcat attcgggaac
     3601 agcttttcca ttgcccgttt catccacaca tcaatcggaa aagcctcaat tctgtgcata
     3661 ccgaaaagaa gtgtgcagtc ggcaaccttt ttgccgacgc ccgtaatttt cataagctcc
     3721 gctcttgcgt cttcatagcc aagcgtgaaa cagctgtcaa gattcacctc gccgtttgca
     3781 accttttgtg ccgcatcaat aatgtaacgg ttgcgaaatc ctgctctcag cggtgcaagg
     3841 tcatcgggcg aaagttttgc aagtgtttcg gctgtcggga acgcaaattc tccgtcatca
     3901 agccgtgtgc cgaagttctc acacaaacgc tcaacaatac ccttgattcg ctttatattg
     3961 ttgttctgcg aaatgatgaa tgtgcagagt gcctcaaaag gctcttgtct taaaatcctt
     4021 atgcccgggg cgtactttgc cgcctcgttc agaacgggat gtattgtgct gatttcttca
     4081 cggatttttc cgtaatcaag cgaaaagtca aagtagctgt accagatgtt tttgaaatcg
     4141 tcagccgttg cattttcaat gtacatatcc gttttgtcaa ggctgacggt tacgcttttg
     4201 ccgtaggcaa caccgtgaaa agaaccgtcg gcctgcggtt tccaacgaaa gctctgaccg
     4261 cagtcgagag tctgcgcaag gtcaaggtca tgcacatcgg ttattttgat tttgttgtcg
     4321 agttcaatac attccatgat tttcaccgcc gaaattttct taataaagta tatcacattc
     4381 gggaaaatta tgatatactt tatttaaaat tgaaaatgga aaattgaaaa tggaaaatga
     4441 atgtcgggaa gatagtttgt ttgtaaaaca gatttcagtc ccgactgtat ttcagacagg
     4501 aactcattga acgcaagcct tgatgaaata ttttgtttaa acacatatgt tttgatatga
     4561 tgaaaacggt gtgttataac caccacatta actcaaaagg aggattattg tgaaagaaac
     4621 tatctgcaca attccgatta acgatatatt tatgccgaaa tgcggttgtc cgttttgccg
     4681 aatggagagt atgcttgagg agcagtatgt aaaattcatt acgggcgatg caatgatgga
     4741 gcctaatatc aggattgaaa ccaacaaaaa aggcttttgt caccgccatt tttcgcagat
     4801 gtttgaaaaa ggacaaaaac tgccgaatgc gcttattctt gaaagtcatc ttcaggaaat
     4861 tattgataac gatatgccga aaaagctgaa gggcaagcct gataagaaac agcttgaagt
     4921 cattaaaaag cgacttgatt cgtgttatgt atgcgacaga atcgagtggg atatgcacca
     4981 tttttgcgac acgatttttg ttgagtgggc aaagggagag gagttttata agctgtacaa
     5041 cgaacagccg tttatctgct ttaaacacta cgattttatt atgaatgttg caacccaaaa
     5101 gagcaagctg ccgtcaaagc ttttaccgca gttctatgcc gaaacagcag gtcttacaaa
     5161 aaaatatctg ctgtcgctta aagaagatat cacatatttc tgctcaatgt ttgattaccg
     5221 caaccacggc aaagattggg gaagctccaa ggattcaatt gagagaagta ttgaattttt
     5281 gacaggcgaa aagccgtaaa cggcgagccg actttttatg ttcgtgaaga tagtttgtga
     5341 atgtcaaaaa acatattatg ccc
//
LOCUS       DAOMVE010000124         5124 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_4530_length_5124_cov_3.129414, whole genome shotgun sequence.
ACCESSION   DAOMVE010000124 DAOMVE010000000
VERSION     DAOMVE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4530_length_5124_cov_3.129414"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1238
                     /locus_tag="PLS64_07295"
     CDS             261..1238
                     /locus_tag="PLS64_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HRL43662.1"
                     /translation="MPLQIVRNDITKMKCDAIVNAANNSLLGGGGVDGAIHRAAGNEL
                     LNECRKLGGCRTGEAKITGAYKLPCKYVIHTVGPVWQGGNYHEEELLSSCYRNSLQLA
                     KAYECESVAFPLISSGVYGYPKEQALQVAINEICKFLADNDMLVYIVVFDKDGFRVSK
                     KLVRDIAEYIDEHYVETHYSENRSRGLSRLLRQPETYPMQTASLNLADVVNQLDESFS
                     QMLLRKIDEKGLTDSQCYKKANVDRKLFSKIRNNVNYKPKKTTAIAFAVALELSLDET
                     KEMLQKAGYALSHSNKFDVIIEYFIQKGEYDIFTVNEALFEFDQVLLGQ"
     gene            1328..1972
                     /locus_tag="PLS64_07300"
     CDS             1328..1972
                     /locus_tag="PLS64_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRL43663.1"
                     /translation="MKNNITELVFILDRSGSMSGLEQDTIGGFNSLIEKQRRQNGKCY
                     VSTVLFDHETEVLHDRVELSEISKMTENDYTVRGSTALIDAIGGAIHHIGNIHKYARK
                     EDVPEHTLFVIMTDGMENASRIYSSNKVKKMIERQKNRYGWEFLFIGANIDSVETAKH
                     FGINSDRSVNYHADGQGTAVVFDAVSKTVCNFRKSRPLSSSWSDEIDKDYESRK"
     gene            complement(2026..3393)
                     /locus_tag="PLS64_07305"
     CDS             complement(2026..3393)
                     /locus_tag="PLS64_07305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRL43664.1"
                     /translation="MMHKLKVRANHTVYIIFAVLSLFSLTLIFNDNIWFDEAYTLSLI
                     QHNYSDIINILKSDMHPPLYFLSLKTFCCIFGYSITATKIFSAIGYIATLFLGCTIIK
                     KHYGSKTSIIYMLTVGAVPMMLYFSVQQRSYSWSIFFVTLCFIESLLFIANKKFHHCI
                     LFAIAGLGAAYNHIYALLAVAIIFAFANIYLLIKDRNLFKRVIIADLIMVAGYSFWII
                     PLLNQTKSASSNFWLSGVEPLSVIVFISGIAVSALVLMKKSNRKLCIIFADVCVMGIQ
                     IIGLFVTVFIRPFYIARYSVVILGIFAILVAFGVKDIKPKPSKVICTLLCVVNIGCLV
                     ATGLFEYNPSMTNFRERFSSQQSESDTFVYCDSAFGIMSYYYPNNTHLCTYKENWFEA
                     FENVECINKNEIADKVDPNHKIWFVKNELTKMPKCIKSNFKYKKIDSFQCDFNKFDLY
                     LLILK"
     gene            complement(3487..3819)
                     /locus_tag="PLS64_07310"
     CDS             complement(3487..3819)
                     /locus_tag="PLS64_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRL43665.1"
                     /translation="MKISKELIKGSTAMLVMSVLKKSDMYGYKIIKELELQSESVFEL
                     KEGTLYPILHALEEENLLESYWEEFDGRKRKYYHLTRKGTKRLKEKQEEWKVYSKSVE
                     KVLSFSGV"
     gene            complement(3839..>5124)
                     /locus_tag="PLS64_07315"
     CDS             complement(3839..>5124)
                     /locus_tag="PLS64_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43666.1"
                     /translation="ATEEMGNPDDTAVPLNALHQNKYCGVFTFICLVAVTALFMATVN
                     ASPLFCYVAEYSNLTHYAMFDFWSLIILATYTVMIIYSQKKSHKSVAIFIIISLGFQV
                     LMSAISITLQGSFLLIIKFSSIFQPAVYTIMTIITKGPIAYIDSLFAYENIEYSAVNE
                     WCYTAIPFIIMALLLLWAIAVLIKIRGNERMKGRKIHLKITKVFKNIFIVFLSVNFLI
                     MGICAVISRVEINSKSTEILAEREKIIDFVMNANLKTTSNEFLDNAKANGFDFQLNFK
                     YGKQPTFDSSDKEYVYRHNGNMISVKDINCNMYLSLDYNALNTSIFYDDLLLTTKEVD
                     AINNLKVGDSISKLYEKSLIYKASAISKIYYANDNLGNPKYSLYPCMTFVIDKKGQTY
                     KTIDISVSDGKISKICEPIEHKKEYETGFNFFIQS"
BASE COUNT         1603 a          905 c          997 g         1619 t
ORIGIN      
        1 ttgatggtag atttcaaaat atcctagcat aatgagaata tccggattgc gatagtaaca
       61 ggcttaattt caatagttct gacaacacga aaaatagaat atatgataat cacaagcgac
      121 agaaattatg ctgtcgcttg tttggctgtt aaaatacaaa ccatactatt taattttgca
      181 ttgaatttaa ttgagaggtg ctatataatt aatatcagag taataaaaca ttaaaattct
      241 gaatttaaag ggtgattatt atgccgttgc agattgttcg gaatgatata actaagatga
      301 aatgtgacgc aattgttaat gcggcaaaca attcgttgct tggcggaggc ggtgttgacg
      361 gtgcgattca ccgtgcggcg gggaacgaat tgcttaatga atgcagaaaa cttggcggtt
      421 gcagaacggg tgaggcgaaa attacgggtg cgtacaaact gccctgcaag tatgttattc
      481 acacggtcgg ccctgtatgg caaggcggaa attatcatga ggaagaactg ctttcgtcat
      541 gttatcgtaa ctcgttgcag cttgcaaagg cttatgaatg cgaatcggtt gcctttcctt
      601 tgatttcatc gggggtttac ggctatccga aagaacaggc attacaggtg gcaatcaacg
      661 aaatctgtaa atttcttgca gacaacgata tgctcgttta tattgttgtg tttgacaaag
      721 acggatttcg tgtcagcaaa aagcttgttc gtgatattgc cgaatacatt gacgaacact
      781 atgttgaaac gcactattcc gaaaaccgca gtcgtggatt atcccgtctt ttaagacagc
      841 cggagactta tccaatgcaa accgcatctt tgaatttagc tgatgttgta aatcagcttg
      901 atgaaagctt ttcccagatg cttttgcgta aaattgacga aaaaggtctt acggattctc
      961 agtgttataa aaaggcgaat gttgaccgca agctgttttc taaaataaga aacaatgtca
     1021 actataaacc taagaaaact accgcaattg cctttgccgt tgcacttgaa ctgtcgcttg
     1081 atgaaactaa agaaatgctc caaaaggccg gctatgcact ttcgcactcg aataaatttg
     1141 atgttatcat tgaatatttt attcaaaagg gcgagtatga tatttttact gtaaacgagg
     1201 ctttatttga atttgaccaa gttttgttgg gtcagtaaat gtcgcatttc aggcgaccat
     1261 atgttatccc gaaagttgta tgattaagtc aagataaaaa acttaattac ttttggaggt
     1321 aacaactatg aaaaacaaca ttacagaatt ggtattcatt cttgacagaa gcggttcaat
     1381 gtcaggactt gaacaggaca caatcggcgg atttaattcg ctcattgaaa agcaacgcag
     1441 acagaacggc aagtgctatg tttcaaccgt attgtttgat cacgaaaccg aggttcttca
     1501 cgacagggtt gaattgtctg aaatttcaaa aatgactgaa aacgattata cggttcgtgg
     1561 ctcaactgca ctcattgacg ctatcggcgg tgcaattcat cacattggca acattcataa
     1621 gtatgcaaga aaagaagatg ttcccgaaca caccttgttt gtgattatga ccgacggaat
     1681 ggagaacgca agccgtatct attcaagcaa taaggtcaag aaaatgattg agcgtcagaa
     1741 aaatcgctac ggctgggaat ttctttttat cggagctaac attgattccg ttgaaaccgc
     1801 taagcatttt ggaatcaatt ccgaccgctc cgttaattat cacgctgacg gtcagggtac
     1861 ggctgttgtt tttgacgcag tatcgaaaac cgtgtgcaat ttcagaaaaa gcagaccgct
     1921 ttcatcgtct tggagtgatg agatagacaa ggactacgaa agcagaaaat aagaggcaaa
     1981 ttagttttaa tttctgcatt tataccataa acaaaagacc tatatttact ttagaataag
     2041 taaatatagg tcaaatttat tgaagtcaca ttggaaagaa tcaatctttt tatacttgaa
     2101 attactttta atgcattttg gcatttttgt gagttcattt tttacaaacc aaattttatg
     2161 atttgggtca actttgtctg caatttcatt tttgtttata cattcaacat tttcaaatgc
     2221 ctcaaaccag ttttctttgt aggtgcaaag gtgggtgttg ttcggataat aatacgacat
     2281 aattccgaat gcactgtcgc agtaaacaaa agtgtctgat tctgactgtt gagaactgaa
     2341 tctttcacga aaatttgtca ttgacggatt atattcaaac aatcctgttg caacaagaca
     2401 gccgatattt acaacgcaaa gcagggtgca tattactttg ctcggctttg gttttatatc
     2461 ttttacgccg aaagcaacga gaattgcaaa gataccgaga attacaacgc tgtatcttgc
     2521 aatgtaaaac gggcgtataa aaacggttac gaacaacccg attatttgaa tccccataac
     2581 gcagacatct gcgaaaatta tacacaattt tcggttgctc tttttcataa gaacaagtgc
     2641 agatactgct atgcccgata tgaatacaat tacagacaac ggttcaactc cgctgagcca
     2701 aaaattactt gaggctgatt ttgtttgatt taaaagagga attatccaaa atgaatatcc
     2761 tgccaccata ataaggtctg caataataac acgcttgaac aaatttctgt ctttaatgag
     2821 cagatatata tttgcaaaag caaagataat tgcaactgca agaagtgcgt agatgtgatt
     2881 gtatgcagcc ccaagtcctg ctatagcaaa taaaatgcag tgatgaaact ttttgtttgc
     2941 tataaaaagc agggactcaa taaagcataa tgtaacaaaa aatatgctcc atgagtatga
     3001 cctttgctga acgctgaaat agagcatcat tggcacagct ccgacagtca gcatataaat
     3061 tattgatgtt ttactgccgt agtgcttttt tatgattgtg catcccaaaa acagcgttgc
     3121 aatgtaaccg attgccgaaa atatttttgt tgctgttatt gagtatccaa agatgcaaca
     3181 aaatgttttc agcgacaaaa aatacagcgg tgggtgcata tcggatttca gaatattgat
     3241 tatgtctgaa taattgtgct gaatcagcga cagcgtgtac gcctcgtcaa accatatatt
     3301 gtcattaaat ataagtgtca gggaaaacaa tgacaaaacg gcaaatatga tatataccgt
     3361 atgatttgct ctgactttta atttgtgcat cataatatct ccgtttctga gtttatagga
     3421 taattttacc attacagggc tataaattca agattaacgg aaaacggtct gtctgtgata
     3481 agtgttttac acacctgaaa agctgagaac tttttcgact gattttgaat atactttcca
     3541 ttcctcctgc ttttctttaa ggcgttttgt gcctttgcgg gtgaggtggt agtatttacg
     3601 ctttctgccg tcaaattctt cccagtagct ttcaagcaga ttttcttctt caagtgcgtg
     3661 aagaatgggg tagagagtac cctctttaag ctcaaacacg ctctcgctct gtaattcaag
     3721 ctctttaata attttgtatc cgtacatatc gcttttcttt aaaacgctca taacaagcat
     3781 tgctgtactg cctttgataa gttctttact gattttcatt ttatttgctc ctttccgatt
     3841 agctctgaat gaaaaaatta aatccggttt cgtattcttt tttatgttca ataggttcgc
     3901 atatcttgga tattttaccg tccgaaacac ttatgtcaat tgttttgtat gtttgacctt
     3961 ttttgtcgat aacaaatgtc atacaggggt acagactgta tttcgggttt ccgagattat
     4021 cattagcata gtatattttt gagattgccg aagccttata gataagagat ttttcgtata
     4081 atttactgat tgaatctcca accttgagat tatttattgc gtcaacctct ttcgtggtta
     4141 ataacaaatc gtcatagaaa attgatgtgt ttaaagcgtt ataatctaaa gatagataca
     4201 tattgcaatt gatgtctttg actgaaatca tattgccgtt atgccgatat acatactctt
     4261 tgtctgaaga atcaaatgtc ggctgtttgc cgtatttgaa attcagttgg aagtcaaatc
     4321 cgtttgcttt tgcattgtcc aaaaattcat tgcttgtggt ttttagattg gcattcatca
     4381 caaaatcaat tattttttct ctttcggcaa gaatttcggt agattttgaa tttatctcta
     4441 ctcttgaaat aaccgcacaa attcccatga tgagaaaatt gacagagaga aatacaatga
     4501 aaatattttt gaatacttta gttattttca gatgaatttt tctgcctttc atccgctcgt
     4561 ttcctctgat tttaatcaga accgcaattg cccacagtaa caacagtgcc ataattataa
     4621 aaggaattgc cgtgtagcac cactcattga ctgccgaata ctcaatattt tcatatgcaa
     4681 acagactgtc aatatacgca atcggacctt ttgtgatgat agtcataatc gtataaaccg
     4741 caggttggaa gattgacgaa aatttgatta taagaagaaa tgagccttgc aatgttattg
     4801 agattgcaga cattaaaacc tgaaagccaa gcgatattat gatgaagatt gcaaccgatt
     4861 tgtgactttt cttttgactg taaattatca tcaccgtgta tgtggcaaga attatcagcg
     4921 accaaaaatc aaacattgcg taatgcgtca agtttgaata ttctgcaaca tagcagaata
     4981 gaggtgatgc gtttacggtt gccataaaaa gtgcggtaac agccacaaga caaatgaatg
     5041 tgaatactcc acaatattta ttttggtgaa gagcattcaa cggcacggcg gtgtcatcgg
     5101 gattgcccat ttcttcggta gcac
//
LOCUS       DAOMVE010000125         4870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_4902_length_4870_cov_2.598754, whole genome shotgun sequence.
ACCESSION   DAOMVE010000125 DAOMVE010000000
VERSION     DAOMVE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4902_length_4870_cov_2.598754"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /gene="sufB"
                     /locus_tag="PLS64_07320"
     CDS             <1..1036
                     /gene="sufB"
                     /locus_tag="PLS64_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRL43667.1"
                     /translation="YDSELVYHNVREEVAAQGVVYTDMESALKGEYADMVKKYFMKLV
                     RPNDHKFAALHGAVWSGGSFVYVPPGVSVEIPLQSYFRLNAPGAGQFEHTLIIVDEGA
                     DLHFIEGCSAPKYNIANLHAGCVELFVKKNARLRYSTIENWSKNMYNLNTKRALVEDG
                     GTMEWVSGSFGSHVSYLYPMTILKGDRSRMEFTGITFAGKGQNLDTGAKVVHIGKETS
                     SFMNTRSISKDGGISTFRSSVVVNKSAENAKSAVSCQSLMLDSKSRSDTIPVMDIRTP
                     HADIGHEAKIGRISDEAVFYLMSRGLSEEDARAMIVSGFADNVSKELPLEYAVEMNNL
                     IQLEMKGSIG"
     gene            1047..2132
                     /locus_tag="PLS64_07325"
     CDS             1047..2132
                     /locus_tag="PLS64_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRL43668.1"
                     /translation="MVDNKDLTVNFLPSPTWNRLGVNRAKIRNIPVDGWNSIPVQKEI
                     IEKYTNISDSKIRDSFANIQTGMGEEIDEISKISQSEKIRISADKTKSEKLFFNCKNG
                     ENAFADVELYAPENTELTVFMTMQSAWNANGICAVRTKFKAEKDAKIRLVQLNLLSQN
                     FRFINDVGAECEDNAAAEILQLFIGGNETYTGTKTTLLGRQSNLDLNVGYYCKDEQLL
                     DMNYVAEHIGKKTVSSMNAGGVLRDKAHKLFRGTIDFPLGSSGAKGDENEDVLILGED
                     VINQTVPLILCAEEDVEGNHGASIGRPSDDVLFYLASRGLDEEEACNLMARAKLDALC
                     SKIGDEELETLAKSCLEEVTGYANEKL"
     gene            2113..3330
                     /locus_tag="PLS64_07330"
     CDS             2113..3330
                     /locus_tag="PLS64_07330"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HRL43669.1"
                     /translation="MQMKNFKEDFPLLRENPVVYLDSAATAQRPESVINSEMEFYKKC
                     NANPLRGLYDLGFKATECYEQSRETVRKFINARSEREIIFTRNATESLNLVAYSYGMN
                     FLKKGDEILVTVMEHHSNQLPWRVVAEKTGAAVKYIECNPDGTITEEQLKQAFSERTR
                     LVAVTHISNVLGCKTPIKRITELAKECGAVVVLDASQSVPHIPVDVQSLDVDFLAFSG
                     HKLMAPFGIGVLYGKESLLEKMPPFLTGGEMIDSVTRDKVIYSELPHKFEAGTVNAAG
                     AWGLKTAIEYIQNIGFDTIGGIEEELTKRAFDGLMKIPHINIQGSNNPKEHCGIISFT
                     IDGVHPHDVSSILDADGIAVRAGHHCAQPLMEFLGIPSTTRASIYFYNTKDDIDAFLN
                     SVSSVRRRMGYGK"
     gene            3320..3745
                     /locus_tag="PLS64_07335"
     CDS             3320..3745
                     /locus_tag="PLS64_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HRL43670.1"
                     /translation="MENNRSFYNEILTDHNLHPMHKHPLDTATASLEGVNPSCGDDIV
                     LNLNIKDGVITDGSFTGDGCAISQASADIMLDLIIGKTVDEAEHLKDIFLKMIQGKAT
                     DEEIEELEEGGALSDISHMPARVKCAVLGWHTFENIVKE"
     gene            3914..>4870
                     /locus_tag="PLS64_07340"
     CDS             3914..>4870
                     /locus_tag="PLS64_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43671.1"
                     /translation="MKKKCTLVLISVLTVACIVFAYLLFFYNPSFNMVYDSDTDSYFN
                     NSYLSYNDGTLAAADYRKTKVTAYDSKNNSTVNLPSNGCLINDNLFYINGNKLCCLDT
                     TTNTRKIIDTDCRSFVCNNEVIAYTKNDSVILKNSDTLENIGDIKFDNQIYYINISDG
                     NLYIAERIFEDKTDEYGYSFKVGKQYIFKKYDLKSCKLLKSKNANYVNGIRYVTVCKD
                     TFYFFCDETQTVNNVCLDKDVNYPTIQHPDVKFITSNNDCVYYISEKTESAIIRKTVE
                     SPYNGIWKLEVGSNKPVKIADKCDCDELLATKNFLYCYTINYI"
BASE COUNT         1582 a          907 c         1054 g         1327 t
ORIGIN      
        1 gtatgattcc gagcttgtgt accacaatgt tcgtgaagag gttgccgcac agggcgtagt
       61 atataccgat atggagagtg cgttaaaggg cgaatatgcc gacatggtaa aaaaatattt
      121 tatgaaactt gtccgtccga atgaccacaa atttgccgca cttcacggtg cagtatggtc
      181 aggaggttcg tttgtgtatg ttccgccggg agtatcggta gaaattccgt tgcagtcata
      241 tttcagactt aatgcacccg gtgcaggtca gtttgaacac acgctcatta tcgttgacga
      301 gggtgcagac ctgcatttta tagagggttg ttctgctccg aaatataaca tagcaaatct
      361 tcacgcagga tgcgttgagc tttttgtaaa gaaaaatgca agactcagat attcaacaat
      421 tgagaactgg tcaaaaaata tgtacaacct taacaccaaa agagcacttg ttgaagatgg
      481 cggtactatg gaatgggtat cgggttcatt cggttctcat gtgtcatatc tttatccgat
      541 gacaatttta aaaggcgacc gttcaagaat ggaatttacg ggcattacat ttgcaggcaa
      601 gggacagaac cttgatacag gcgcaaaagt agttcatatc ggcaaagaaa catcttcgtt
      661 tatgaacaca aggtcaattt caaaagacgg cggtatcagc acattcagaa gctccgttgt
      721 tgtaaacaaa tctgccgaaa atgcaaaatc tgccgtttcg tgtcagtcgc ttatgcttga
      781 ttcaaagtca cgctcagata ctattcctgt aatggatatc aggactccgc acgcagatat
      841 cggtcacgag gcgaaaatag gcagaatcag cgatgaggct gtattctacc ttatgtcaag
      901 aggtcttagt gaagaggatg caagggcgat gatagtcagc ggttttgcag acaatgtgtc
      961 aaaggaactt ccgcttgaat atgcggttga aatgaacaat ctcattcaac ttgagatgaa
     1021 gggcagtatc ggataaggag gacattatgg ttgataataa agacttaaca gtgaactttc
     1081 ttccgtcacc cacttggaac cgattgggag tgaatcgggc aaaaatccga aatattcccg
     1141 ttgacggttg gaacagtatt cctgttcaaa aagaaataat cgaaaaatat acaaatattt
     1201 cagattcaaa aatccgggat tcatttgcaa atattcagac aggtatgggc gaggagattg
     1261 acgaaatctc aaaaatatca cagtctgaaa aaatcagaat atcggctgat aaaacaaaaa
     1321 gcgaaaagct gttttttaat tgcaaaaacg gtgaaaacgc ttttgcagat gtagaacttt
     1381 acgctcccga aaacacagaa cttaccgtgt ttatgacaat gcagtcagcg tggaatgcaa
     1441 acggtatttg tgccgtaaga acgaaattta aagctgaaaa agacgcaaaa atcagactcg
     1501 tacagcttaa tctcctttcg caaaatttcc gctttataaa cgatgtcggt gcagaatgtg
     1561 aagataatgc agccgcagag atattacagc tttttatcgg cggaaatgaa acctatacag
     1621 gcacaaagac aacgcttttg ggcaggcaga gcaatcttga cctcaatgtc ggctactatt
     1681 gcaaggacga gcagttgctt gatatgaact atgttgccga gcatatcggc aaaaagaccg
     1741 tcagctcaat gaatgccgga ggtgttttga gagataaagc acataaatta ttccgtggta
     1801 caattgattt cccactcggc tcatcgggtg caaaaggtga cgagaacgaa gatgttttaa
     1861 ttttaggcga agatgtaatc aatcagaccg taccgctcat tctttgtgcg gaagaggatg
     1921 ttgagggcaa ccacggtgcg tcaatcggca gaccgtccga tgatgtgctt ttctatcttg
     1981 caagcagagg tcttgacgaa gaagaggctt gtaaccttat ggctcgtgca aaacttgacg
     2041 ctttatgctc aaaaatcggt gatgaggagc ttgaaacgct tgcaaaatcc tgtctggagg
     2101 aggttacagg ctatgcaaat gaaaaacttt aaggaagatt ttccgctttt gcgagaaaat
     2161 cctgtagtct atctcgacag tgccgcaact gctcaaagac ctgaatcagt tataaattcg
     2221 gaaatggagt tctacaaaaa atgcaatgca aatcctctgc gtggacttta tgatttgggt
     2281 ttcaaagcta cagaatgtta tgagcaatcc cgtgaaacag tcagaaagtt cattaatgca
     2341 aggtcggagc gtgaaataat atttacacgc aatgcaaccg aaagcctcaa tcttgttgcc
     2401 tacagctacg gaatgaattt tctaaaaaaa ggtgacgaaa ttcttgtcac tgtaatggaa
     2461 caccacagca accagttgcc atggagagtt gttgcagaaa aaacgggcgc agcggtaaag
     2521 tacattgaat gcaaccctga cggcacaatt accgaagaac agctgaaaca ggctttttcg
     2581 gagcgtacaa ggcttgtggc agtaactcac atatcaaatg tactcggttg caaaactccc
     2641 ataaagcgta tcacagagct tgcaaaggaa tgcggtgcag ttgttgtgct tgacgcatca
     2701 cagagcgttc cgcacattcc cgttgatgtt cagtcgcttg atgtagattt tcttgcattt
     2761 tccggtcaca aacttatggc acccttcgga atcggtgtat tgtacggcaa agaaagcctt
     2821 cttgaaaaaa tgccgccgtt tttaacgggc ggtgagatga ttgattccgt tacaagggac
     2881 aaggttatct attccgaatt accgcataaa ttcgaggcag gaacggtaaa tgcggctggc
     2941 gcatgggggc tgaaaaccgc aattgaatat attcaaaata ttggttttga tacaatcggc
     3001 ggtattgaag aagagcttac caaaagagcc tttgacggac ttatgaaaat tcctcacatc
     3061 aatattcagg gaagcaacaa tccgaaagag cattgcggaa ttatcagctt tacaattgac
     3121 ggagttcatc cgcatgatgt cagcagtatt ctcgacgctg acggtattgc agtccgtgca
     3181 ggacaccatt gcgcacagcc tttgatggaa tttttaggca ttccgtcaac aacaagggca
     3241 agcatttatt tctacaatac gaaagatgac attgatgcgt ttttgaacag cgtgtcatct
     3301 gtcagaagga gaatgggata tggaaaataa cagaagcttt tacaatgaaa tcctgaccga
     3361 tcacaatctt catccaatgc acaaacatcc gcttgatacg gcaacggcaa gccttgaggg
     3421 tgtgaatcca agctgcggtg atgacattgt gcttaatctt aacatcaaag acggtgtgat
     3481 tacagacggt tcgtttacag gtgacggctg tgcaatttcc caggcgtcag ccgatattat
     3541 gcttgatttg attatcggca aaacagttga tgaagccgaa catctgaaag atattttcct
     3601 taaaatgatt caaggcaaag caactgacga agaaattgaa gagcttgagg agggcggagc
     3661 attgtctgac atttctcata tgcctgcccg tgtaaaatgt gcagttctcg gttggcacac
     3721 atttgaaaac attgtaaaag aataatttac tgaatttaat attttataaa acacattcgg
     3781 ggaacagata aaataatttc tgttccccga tttttattta aaaaatcggg attaatgtat
     3841 taaaatgact aatacaaatc ttgacaaata ttgctttaaa atagtataat aaaagcgcaa
     3901 agaggtgata cttatgaaaa agaaatgtac tctcgtttta atatccgtat tgacagttgc
     3961 atgcattgta tttgcatatt tgcttttctt ttataatcct tcatttaata tggtttacga
     4021 ttccgacacg gattcatatt ttaacaacag ttacctgtcc tataatgacg gaacgcttgc
     4081 agcggcagat tacagaaaaa ctaaggttac ggcttatgat tcaaaaaaca attcaaccgt
     4141 aaatttgcct tcaaacggtt gtctgattaa cgataattta ttctatatta acggcaataa
     4201 gctctgctgt cttgatacaa caacaaacac acgaaaaatc atagacaccg attgccgaag
     4261 ttttgtttgt aacaatgaag ttattgctta cacaaagaat gattccgtaa tattgaaaaa
     4321 ctctgatacc cttgaaaata taggggatat taagtttgat aatcagattt attatataaa
     4381 tatttcagat ggcaatctct atattgcgga gcgtattttt gaggataaaa cagatgaata
     4441 cggttacagc ttcaaagtag gaaagcagta tatttttaaa aaatatgacc taaaatcctg
     4501 caaattgctc aaaagtaaaa atgcaaatta tgtaaacgga attcgctatg taacggtatg
     4561 caaagatact ttctatttct tttgcgatga aacccaaacc gtaaacaatg tatgtcttga
     4621 taaagatgta aattatccga caattcagca tcccgatgtt aaatttatta catccaataa
     4681 cgattgtgtt tactacatat ccgagaaaac agaatcggcg ataatacgca aaacggttga
     4741 aagcccttat aacggtatat ggaaactcga agtcggctct aataaacctg ttaaaattgc
     4801 tgacaaatgc gactgtgacg aactgcttgc aacaaagaat ttcttgtatt gttacacaat
     4861 taactacatt
//
LOCUS       DAOMVE010000126         4866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_4905_length_4866_cov_3.362087, whole genome shotgun sequence.
ACCESSION   DAOMVE010000126 DAOMVE010000000
VERSION     DAOMVE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4905_length_4866_cov_3.362087"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2297)
                     /gene="pulA"
                     /locus_tag="PLS64_07345"
     CDS             complement(<1..2297)
                     /gene="pulA"
                     /locus_tag="PLS64_07345"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HRL43672.1"
                     /translation="MMITGMCAADVSAAEKKTVTDYQAYAANLDKSAYSGNDLGASYS
                     KKATTFKVWSPNAASVRVNIFEHGSDNEGDAGSIMSRAMSLDKTTGVWSVTINGDLLN
                     KYYTYSVTHGKTTKETADVYAKACGVNGQRSMVVDLSTTNPDGWENDKHVLVQNQTDA
                     SVWEISVADFSSSESSGVSEANRGKYLAFTEEGTTVNGVQGASSTCVDYLKKLGVKYV
                     QIMPFYDFGSVDESKNIMDQYNWGYDPVNYNCPEGSYSTNPKKGEVRIKECKQMIQAL
                     HNAGIGVIMDVVYNHTYTSDSWLQRTVPNYYYRMNNDGTFSNGSGCSNDTASEHLMFR
                     KYMIDSVTYWASEYHIDGFRFDLMGLHDVTTMNSIRTALDNLYADGSGSQILMYGEAW
                     DMATNCDEGTVLASQKNLKQLSDRIGAFDDTIRDAIKGSTGGTDGAFVQEGSRRANLK
                     TGIAGQSDTTTGWANVPSQCVTYASCHDNLCLYDKLVGSVYGADGKYRKRYEDLVAMN
                     KLSAAIVITSQGIPFSLGGEEFCRSKDGDENSYASSRKENMLDWENVDLYSDVIEYYR
                     GLYKIRDAFAAFSDSTAATANSLTYLSDVPKGVMGYTINNTESGKWSQMCVIFNGSDS
                     AQNVTAKGDWVVLADNKTAGLRNIKNVTNSVKVEAHSAVIMVDTKSYDSAGIMDDEGA
                     VVIDYYDNKTEKLIKSQTLTGELGTSYDLTNLASTLNYDVKKTDGEIKGVFTDQVGHA
                     KVYVEEYDGEMSTVTVKFVDETNNT"
     gene            complement(2693..3949)
                     /gene="eno"
                     /locus_tag="PLS64_07350"
     CDS             complement(2693..3949)
                     /gene="eno"
                     /locus_tag="PLS64_07350"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRL43673.1"
                     /translation="MEIKIDTIIGREIIDSRGNPTVEAEVGLSDGTFAKASVPSGAST
                     GEFEAVELRDGEKRYLGKGVQRAVENINSIISPNLCCDSPFDQCAIDSKMIELDGTEN
                     KELLGANAILAVSLACAKAAAKNLRLPLFRYIGGTYAVRMPVPMMNILNGGAHASNNI
                     DIQEFMIMPVGARCFSEGMMQCCEIYHTLGKILKENNMSTGVGDEGGYAPSLKSDEDA
                     IEYIIKAIEKTGYTTDEIKIALDAASSEWYSDGKYILPKRGDSLSSDELINYFDKLCS
                     DYPIISLEDPLSEHDWDGWQNITSKIGNKVQLVGDDLFVTNTKRLKKGIALGAGNAIL
                     IKINQIGTLTETLDAIDTAHKAGYRTIISHRSGETEDTTIADIAVAVNSGQIKTGAPC
                     RTDRVAKYNRLLRIESAIVNTSTYGL"
     gene            4128..4433
                     /locus_tag="PLS64_07355"
     CDS             4128..4433
                     /locus_tag="PLS64_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRL43674.1"
                     /translation="MEMVQYTVRPGNTLFGIAQFFQTTVNDILKYNNIQTPDAIFVGQ
                     VLTVPAGSAETQYHVARPGDSLWIIAQMHGTTVENLSRLNGLSNPNVIYPGQIIKVR"
     gene            complement(4488..>4866)
                     /locus_tag="PLS64_07360"
     CDS             complement(4488..>4866)
                     /locus_tag="PLS64_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HRL43675.1"
                     /translation="LIMVTFEECAQMLDEIADSMPYELYRDLNGGISLLPQLKVHPKA
                     LNNDLFILGEYIRNSLGNAIVFYYGSINRVYGSLDNEAYRKKLVEILHHEVRHHNEYL
                     AGCDDLGIFDRNQIDDYLRKHRK"
BASE COUNT         1329 a         1109 c          926 g         1502 t
ORIGIN      
        1 tccgtattat ttgtttcgtc aacaaacttt acggtaaccg ttgacatctc accgtcatat
       61 tcttcaacat aaacctttgc atgacctacc tgatccgtga acacaccttt gatttcaccg
      121 tcggttttct taacatcata attaagagtt gacgcaagat ttgtcaaatc atagcttgtg
      181 ccgagttcgc ctgtgagcgt ctgtgacttg atgagttttt cggttttgtt gtcataataa
      241 tcaatgacaa cagcgccctc atcatccata attcccgcag aatcatagct ctttgtgtca
      301 accataataa cggctgaatg agcctcaacc tttacgctgt ttgtaacatt ctttatgttg
      361 cgaagtcctg ctgtcttgtt atctgcaaga acaacccagt caccttttgc ggtaacattc
      421 tgtgcagaat cggagccgtt gaagattacg cacatttgac tccattttcc gctctcggtg
      481 ttatttattg tgtagcccat aacacccttt ggaacatcgg aaagatatgt aaggctgttg
      541 gctgttgcag cagtagaatc cgagaatgcc gcaaaagcgt cacggatttt ataaagacct
      601 ctgtaatatt caattacatc tgaatagaga tcgacatttt cccagtcaag catattttct
      661 tttctgcttg acgcatagct gttttcatca ccgtctttac tgcgacaaaa ctcctcaccg
      721 ccgagtgaga acggaatacc ctgtgaagta ataacaattg ccgcagaaag cttgttcatt
      781 gcaacaagat cttcatatct tttgcgatat ttaccgtcgg caccgtaaac cgagccgaca
      841 agcttatcgt aaagacaaag gttatcgtga catgacgcat atgttacaca ttgtgacgga
      901 acatttgccc agcctgttgt tgtgtcactc tgacctgcta tgcctgtttt caggttggct
      961 ctgcgtgaac cttcctgaac aaaagcaccg tccgtaccgc ctgtactgcc cttgattgca
     1021 tcacggattg tatcatcaaa cgcaccgatg cggtcgctca actgcttgag atttttctga
     1081 cttgccaata ctgtgccttc gtcacaattg gttgccatat cccacgcctc accgtacata
     1141 agaatctgac tgccgctgcc gtctgcataa agattgtcaa gtgcggtacg aattgaattc
     1201 atagttgtta catcatgaag tcccataagg tcaaagcgga aaccgtcaat gtgatattcg
     1261 cttgcccaat atgtaacgga atcaatcata tactttctga acataagatg ttcgcttgct
     1321 gtatcgtttg aacagcccga accgtttgag aaagttccgt cattgttcat tctgtagtaa
     1381 taattcggaa ctgttctctg aagccaagaa tcagaggtgt aggtgtggtt atagacaaca
     1441 tccataatta caccgattcc ggcattatga agtgcctgaa tcatctgctt acattccttg
     1501 attctcactt cacccttttt aggatttgtt gaataagaac cctcggggca gttgtagttg
     1561 acgggatcat agccccagtt gtactggtcc ataatgttct tgctctcgtc aacagaaccg
     1621 aaatcataga acggcataat ctgcacatac ttaacgccga gctttttcag atagtcaacg
     1681 caggttgatg acgcaccctg aacaccgttg actgttgtac cttcttcggt aaaagcaagg
     1741 tacttgccac ggttagcctc actcacgccg cttgattcgg atgatgaaaa gtctgctaca
     1801 gagatttccc agaccgacgc atcagtctga ttctgtacaa gcacatgctt atcattttcc
     1861 caaccatcgg gattggttgt gcttaaatcg acaaccattg agcgttgacc gttcacaccg
     1921 cacgcctttg catagacatc ggctgtttcc ttggtagttt ttccgtgtgt taccgagtag
     1981 gtataatatt tgttaagcag gtcaccgtta attgtaacag accacacgcc cgttgttttg
     2041 tcaagcgaca tggctcttga cattatagaa cctgcatctc cctcattatc actgccgtgt
     2101 tcaaaaatat tcacacgaac agacgccgca ttaggcgacc ataccttgaa ggtagttgcc
     2161 ttttttgagt aagaagcccc caaatcatta cctgaatatg ccgacttatc cagatttgca
     2221 gcatatgcct gataatctgt tacagttttc ttttccgctg ccgaaacatc agccgcacac
     2281 attcctgtaa tcatcatagc tgtcattact cctgccaata atctttttag aaacaatcaa
     2341 aaatgcctcc tgacttattc ggatatgagc aaaacaaagg ttacaatcag cactgtttta
     2401 caatagtcca tacgaattcc aatgttagca tttcattagt taattttatc atataaggca
     2461 atatatttta attccgatta agaatatagc aattgataaa ggctttattg ttgtagaatt
     2521 ttctttgaca aatatgtgca atatgctgat tttctaaaag tttattttta aatttatatt
     2581 aagcaaagaa aaatctgtca aaaaaataca attcgacagt taaatttact aaattattga
     2641 attttgttat caatagaaaa aaattccccg accgcagtcg gggatgacag aatcacaatc
     2701 cgtaggttga agtgttgaca attgcactct caatccgaag aagtctgtta tattttgcaa
     2761 ctctgtcggt tctgcacgga gcacctgttt ttatctgtcc cgaattaacc gcaacggcaa
     2821 tatccgcaat ggttgtgtcc tccgtttcgc cgctcctgtg tgaaataatc gtgcgataac
     2881 ccgccttgtg agccgtgtca attgcatcaa gcgtttccgt cagcgttcct atctgattaa
     2941 ttttaatcaa aattgcgttg cctgctccga gtgctatgcc ttttttaagt ctttttgtgt
     3001 ttgtaacaaa caaatcatcg ccgacaagct gaactttgtt gccgattttt gaagtaatat
     3061 tctgccaacc gtcccaatcg tgttcggaaa gcggatcttc aagcgaaata atcggataat
     3121 cagaacagag cttgtcaaaa taattaataa gttcgtcgct tgaaagcgaa tcgcctctct
     3181 ttggaagaat atattttccg tcggaatacc attcggaaga tgcggcatcg agcgcaattt
     3241 taatttcatc ggtagtataa cctgtttttt caatcgcttt aattatatac tcaattgcat
     3301 cctcatcact tttcaggctc ggtgcataac cgccctcatc acctacacct gttgacatat
     3361 tattctcttt tagaattttg ccgagtgtat ggtagatttc acagcactgc atcattccct
     3421 cggaaaaaca ccttgcgccc acaggcataa tcataaattc ctgaatgtcg atattgtttg
     3481 acgcatgagc gccgccgttg agaatattca tcattggaac aggcattctg accgcatatg
     3541 ttccgccgat ataacggaat aacggcagtc tgagattctt tgccgccgcc tttgcacagg
     3601 caagcgatac ggcaagaata gcatttgcac cgagcaattc cttattttcc gtgccgtcaa
     3661 gttcaatcat tttgctgtcg attgcacact ggtcaaaagg tgagtcgcaa caaagattag
     3721 gcgaaattat gctgttgata ttttcaacgg ctctctgcac acctttgccg agatagcgtt
     3781 tttcaccgtc acgaagttca accgcctcaa attctcccgt tgacgcaccc gaagggacag
     3841 acgcttttgc aaaggttccg tcggaaagtc cgacctccgc ctcaaccgta ggatttcctc
     3901 ttgaatcaat tatttctctg ccgattatcg tatctatttt tatttccatt tatatgccct
     3961 ctcgtattga aattttaaaa cataaaccgt tttcagatat ttttaatctg agcaacgctg
     4021 tgttttgtct gcattgttat ttttccgtac acggtttatt ttatgcagga gtaacgcatt
     4081 tttgtcacag gcatatattg ttactatgaa aagcaaggag gacagatatg gaaatggttc
     4141 agtacaccgt tcgtcccggc aatacgcttt ttggcattgc gcagttcttt cagacaacgg
     4201 tgaatgacat attaaaatac aacaacattc aaactcctga cgctattttt gtaggtcagg
     4261 ttcttacggt tcccgccggc agtgcggaaa cgcagtatca tgttgcccgt ccgggcgatt
     4321 cgctgtggat aattgcacaa atgcacggaa caaccgttga aaatctctca cggctcaacg
     4381 gactttcaaa tccgaatgta atttatccgg ggcagattat aaaggtaaga taacaaagga
     4441 ggacagcttt ctgtttagaa aagctgtcct cttaaaattt catttgatta tttcctgtgt
     4501 tttctaaggt aatcgtcaat ctgatttctg tcaaaaattc cgagatcatc gcaacctgca
     4561 agatactcgt tatgatggcg aacctcgtga tgaagtattt caacgagctt ttttcggtat
     4621 gcctcattat caagtgagcc gtaaaccctg ttgattgaac cgtagtaaaa cacaattgca
     4681 ttgccgagag aattgcgtat gtactcaccc aaaatgaaca aatcattgtt aagtgctttt
     4741 ggatgaacct ttaactgcgg aagaagtgaa atccctccgt ttaaatctct gtataattca
     4801 taaggcatgg aatctgcaat ttcatcaagc atttgagcac attcttcaaa agtaaccata
     4861 atcaat
//
LOCUS       DAOMVE010000127         4781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5043_length_4781_cov_3.395472, whole genome shotgun sequence.
ACCESSION   DAOMVE010000127 DAOMVE010000000
VERSION     DAOMVE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_4781_cov_3.395472"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLS64_07365"
     CDS             complement(<1..340)
                     /locus_tag="PLS64_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRL43676.1"
                     /translation="MVKMVVFDLDGTLMNSLTDLAISVNYGLKLNGLEEKPVENYKTY
                     VGNGREMLIRRAMGDAWTDHELFEKVKADYDRHYAVHCNDHTAAYDGCAEMLEKLAKK
                     GVKTGVLSNKP"
     gene            complement(351..944)
                     /locus_tag="PLS64_07370"
     CDS             complement(351..944)
                     /locus_tag="PLS64_07370"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRL43677.1"
                     /translation="MYIHDISRDTLTTETYPGDPETVVTPLRSIDEDDECNLSQISMC
                     VHAGTHIDAPLHFCPDGKGITDLRLNTFYGKCTVITVKGVLTGEDMDKLLPYCRKRIL
                     FRGNGKTYLSHSAAIVLAQSRVVLVGTDAESIAPPFDEVKTHLELGRADIAVLENLNL
                     SGVADGEYDLCAFPIKLGGVEAAPCRAILFEQEKGLN"
     gene            complement(1210..3087)
                     /locus_tag="PLS64_07375"
     CDS             complement(1210..3087)
                     /locus_tag="PLS64_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRL43678.1"
                     /translation="MKKSSKVLSLVLAVLMAVSCFSGLTIFSASAEETDTKIYFDASN
                     LPAEWGTTKTVYCHLYAVAGDDLPETSWQGKAEKCKKDTATGLYYFDTAKLKSADGTN
                     HGGLKDNADYAVIFSTIDTKSQSHQTCNVTLGKPCLGDTIYLTGGTVENTEDSSKRDF
                     AATWKNNSDNYGPKAAITSLGHVTEGRFPIYLSRAEMVAQAIFNWAVKNPKNYTPETV
                     ADICAQVEAEPMDVYNAYAEMYATELADPVAYPDCAPLTTVATLLGVDPSGTTAPATE
                     EPTTVEPTTVEPTTVEPTTEPTTEPATEPADATQYVVAGVESLTGYEWQGSPALAPEN
                     VMTKSGDVYTKTFTAVPVGKSYQLKVVANTGDEQKWIGLDGTDNNVTFDVESACDVTV
                     TFNPATNEIAVTGDGVKMVTDLEINSITVVGNGENSWLNGVAWGVDAEVNHMTQIADK
                     VYQITYTGVESADAAYQFKFAVNDDWAANWGLPEQSAATIGKDFDLTFNGENMLLNTV
                     SAGYPEDSLVDVTITLDLTKFDYPSRSGAKANIKIDGNRVPLLGDADGDYSITVVDAT
                     TIQKIAINLMSIAADDANAFKACDANEDGRISIKDATIVQKYIVGGYETGNVGSPISV
                     E"
     gene            3315..4691
                     /locus_tag="PLS64_07380"
     CDS             3315..4691
                     /locus_tag="PLS64_07380"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRL43679.1"
                     /translation="MENSKKTMDKIVALCKNRGFVYPGSEIYGGLANTWDYGPLGVAL
                     KSNIKNAWLKKFVQQNKYNVGLDSAILMNPQTWVASGHLGGFSDPLMDCKECKTRHRA
                     DNLIEDFDGTNVAGWTNEEMMNYIKEKEIPCPNCGKHNFTDIRQFNLMFKTFQGVTED
                     SKNEIYLRPETAQGIFVNFSNIQRTSRKKVPFGVAQIGKSFRNEITPGNFIFRVREFE
                     QMELEFFCKPGTDLEWFEYWRGFCRDWLYSLNIKEENLRLRDHAKEELCFYSKATTDF
                     EYLFPFGWGELWGVADRTDYDLTQHSKTSGKTLEYFDPTTNEKYIPYVIEPSLGVERL
                     FLALVVEAYDEEVIDEKDTRVVLRLHPTLAPYKACVLPLSKKLNEQAGKVYEQLSADF
                     MTDYDDAGSIGKRYRRQDEIGTPFCITYDFESVDDGCVTVRDRDTMQQERVAIDKLND
                     YIAEKIKF"
BASE COUNT         1304 a         1060 c         1013 g         1404 t
ORIGIN      
        1 cgggcttgtt tgaaagcaca cctgttttta cacccttttt tgcaagcttt tcaagcattt
       61 cggcacaacc gtcatatgcg gcggtgtggt cgttgcaatg aacggcatag tgtctgtcgt
      121 aatcagcctt gaccttttca aaaagctcgt ggtcggtcca cgcatctccc attgcccttc
      181 tgatgagcat ttctctgccg tttccgacat atgttttata attttcaacc ggcttttctt
      241 caagaccgtt aagcttcaga ccgtagttca cacttatagc aagatcggta agcgaattca
      301 taagcgtgcc gtcaagatca aatactacca tttttaccat aaatatcacc ttagtttaat
      361 cctttttcct gttcaaagag tattgctctg catggtgccg cctcaactcc gccgagcttt
      421 atcggaaacg cacacaaatc atattcgccg tcagccactc ccgaaagatt gagattttcg
      481 agaacggcaa tatccgctct gccaagttca agatgtgttt taacttcatc aaacggaggt
      541 gcgattgact cggcatccgt accgacaagc accaccctgc tttgtgcaag cacaattgcg
      601 gcagaatgtg aaaggtatgt tttgccgttc ccacgaaaga gtatgcgttt tcggcaatac
      661 ggcaacagct tgtccatatc ttcgcccgtc agcacaccct tgaccgttat tacggtgcat
      721 tttccgtaga aagtgttgag ccttaaatcg gttatccctt tgccgtcagg gcaaaagtgg
      781 agaggggcgt caatatgcgt gcctgcatga acgcacatag aaatctgcga aagattgcac
      841 tcgtcatctt catcaatact tctcagcgga gtcacaacag tttcgggatc acccggatat
      901 gtttcggttg taagagtatc tctcgaaata tcatgaatat acatacatcc ttcgcctctt
      961 ttctttttga acatttttaa ttcacgcaga gcatcagctc ctcagagtct aagtaaaata
     1021 taactctgct taaattcaga acgaacccac cgtatgagta aagcggaaca tctgcactat
     1081 gattatacca catattactg cactttgcac ctttatcggc aaaatttcct tgttttacgg
     1141 caaaaaataa ccccacagga aaaccctgtg gggtaaattg caattgtgaa tttaatcaac
     1201 agcgattaat tattcaacag aaatcggtga accgacattg cctgtttcgt aaccgccaac
     1261 gatgtacttc tgaacgatag tagcatcctt gattgagatt ctgccatcct cgttagcatc
     1321 acatgccttg aatgcgtttg catcatcagc agcaatactc ataaggttga tagcaatctt
     1381 ctgaattgtt gtagcatcta caactgtaat tgagtagtca ccgtcagcat cgccgagaag
     1441 cggaacacgg ttaccgtcaa tcttaatatt agccttagca cctgagcgtg acggataatc
     1501 aaacttagta agatcaagtg tgattgttac atcaacgagt gaatcttccg gataaccggc
     1561 agaaactgtg ttaaggagca tattttcacc attgaatgta aggtcaaaat ctttgcctat
     1621 agtagctgcg ctctgctcag gaagacccca gttagcagcc caatcgtcgt taactgcaaa
     1681 cttgaactgg tatgcagcat cagcagattc aacacctgtg tatgtaatct gataaacctt
     1741 gtcagcaatc tgagtcatgt ggttaacttc agcgtcaacg ccccatgcta caccgttaag
     1801 ccagctgttt tcgccgttac caacaactgt aatgctgttg atttcaaggt ctgtaaccat
     1861 tttaacgccg tcacctgtta cagcaatctc gtttgtagca ggattgaaag ttacagttac
     1921 atcacaagca ctttctacat cgaaagttac attgttgtct gtaccgtcaa gaccgatcca
     1981 cttctgctcg tcgcctgtgt ttgcaacgac cttgagctga tagcttttgc ccaccggaac
     2041 agctgtaaat gtctttgtat aaacatcgcc gctctttgtc attacatttt cgggagcgag
     2101 tgcaggagaa ccctgccatt cataacctgt gagtgactca acacctgcta caacatactg
     2161 tgttgcgtca gcaggctctg ttgcaggttc tgtagtgggt tctgttgtgg gttcaacagt
     2221 tgtaggctca acagttgtag gctcaacagt tgtaggttct tctgttgcag gagctgttgt
     2281 acctgaagga tcaacaccga gaagtgtagc tactgttgta agcggagcac agtcgggata
     2341 agctacggga tcagcaagct ctgttgcata catttcagca taagcgttgt aaacatccat
     2401 tggctcagcc tcaacctgtg cacaaatgtc agctacagtt tcgggagtgt agtttttagg
     2461 attcttaaca gcccagttga aaatagcctg tgcaaccatt tctgctcttg aaaggtaaat
     2521 cgggaatctg ccctcagtta catgaccgag tgatgtgatt gcagcctttg gaccgtagtt
     2581 atcgctgtta ttcttccatg ttgcagcaaa gtcacgcttt gatgaatctt cggtattctc
     2641 aactgtaccg cctgtgaggt aaattgtgtc gccgagacaa ggcttgccga gtgttacatt
     2701 acaggtctgg tgactctgtg atttagtatc aattgttgag aaaattacag catagtcagc
     2761 gttgtcttta agaccgccgt gatttgtgcc gtcagctgac ttcaattttg cagtatcgaa
     2821 gtaataaaga cctgtagcag tatccttctt acatttctct gccttaccct gccaagatgt
     2881 ttcaggtaaa tcatcgcctg ctacagcgta aaggtgacaa tatactgtct ttgttgttcc
     2941 ccactctgca gggagattag atgcgtcaaa atagattttt gtgtcggttt cttcagctga
     3001 tgcagagaat atggtcaagc ctgaaaaaca ggatacagcc ataagaactg caagcaccaa
     3061 actgagaacc ttggatgatt ttttcatcgc taattcctcc ttttttgtta ctcaaatttc
     3121 gagtaattac attatatatc aaaatgctca catttacaag tggtttttgg caagtttgta
     3181 aattttttct aattcttgtt ttaaacctat tgttatgcgc taacatttgg tgtttttact
     3241 tgacaacagt tgcaaataat tgtattattt taatggttat ttttatctat ctatctgtta
     3301 agagaggtta agttatggaa aacagcaaaa aaaccatgga taaaatcgtt gccctgtgta
     3361 aaaacagagg ctttgtttac ccaggctccg aaatttacgg cggtcttgca aacacatggg
     3421 attacggtcc gctcggtgtt gcacttaaaa gcaatatcaa aaacgcatgg ctcaaaaagt
     3481 ttgttcagca gaacaaatat aatgtaggtc ttgactctgc aattcttatg aacccgcaga
     3541 catgggttgc atcgggtcat ctcggcggtt tctctgatcc gcttatggat tgtaaggaat
     3601 gtaaaacccg tcaccgtgcc gacaacctta ttgaagattt tgacggcaca aatgttgcag
     3661 gctggacaaa tgaagaaatg atgaattaca tcaaggaaaa agagattccg tgtcctaact
     3721 gcggcaagca caactttacc gacattcgtc agttcaacct tatgttcaaa acattccagg
     3781 gcgttacaga ggacagcaag aatgaaattt atcttcgtcc cgaaactgct cagggcattt
     3841 tcgtgaactt ctctaacatt cagagaacaa gccgtaagaa agttcctttc ggtgttgcac
     3901 aaatcggtaa atctttcaga aacgaaatca cacccggtaa ctttattttc cgtgttcgtg
     3961 agtttgagca gatggagctt gaattcttct gcaagccggg cactgacctt gaatggtttg
     4021 aatactggag aggtttctgc cgtgactggc tctattctct caatattaag gaagaaaatc
     4081 tccgtcttcg tgaccatgca aaagaggagc tttgtttcta ttcaaaagcc acaacagact
     4141 ttgaatatct cttcccgttc ggttggggcg agctttgggg cgttgccgac agaacagact
     4201 atgaccttac acagcacagc aaaacaagcg gtaagacact tgagtatttt gatccgacaa
     4261 caaacgagaa gtacattccg tatgttattg agccgtcact cggtgttgag cgtcttttcc
     4321 ttgctcttgt tgttgaggct tacgatgaag aagttattga cgaaaaggat acacgagttg
     4381 tgttaagact tcatccgaca cttgctccgt acaaagcttg cgttcttccg ctttcaaaga
     4441 agctcaacga gcaggccggc aaggtttatg aacagctttc agccgacttt atgactgatt
     4501 atgacgatgc cggttcaatc ggcaagcgtt acagaagaca ggacgaaatc ggtactccgt
     4561 tctgtatcac atatgacttt gagtctgttg acgacggttg tgtaacggtt cgtgaccgtg
     4621 acacaatgca gcaggaaaga gttgcaattg acaagctcaa cgattatatc gctgaaaaga
     4681 ttaagttctg attttaatat gatatcaaaa ggacattcca aatggaatgt cctttttttg
     4741 cgtaggggcg gctcaccgta taagaacagc ggataacctt a
//
LOCUS       DAOMVE010000128         4761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5075_length_4761_cov_4.022737, whole genome shotgun sequence.
ACCESSION   DAOMVE010000128 DAOMVE010000000
VERSION     DAOMVE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5075_length_4761_cov_4.022737"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1158)
                     /locus_tag="PLS64_07385"
     CDS             complement(4..1158)
                     /locus_tag="PLS64_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HRL43680.1"
                     /translation="MYNILTLNKIAAIGTSRLGDNYKYGDDVQNPDAVLVRSAAMHDM
                     EFADNLLAIARAGAGTNNIPKDKCSEQGIVVFNTPGANANAVKELVIAGLLISNRKVI
                     EGYEWAKTLKGNGAAVGKMVEKGKSQFVGPEIKGKTLGVIGLGAIGILVANAAVALGM
                     NVIGFDPFMSVGNALKLAPTVKLMKSAEDVMVNCDYLTIHVPLTDDTRDMVDADMIAK
                     MKDGVRILNFSRDGLVNSTAVLEAVKSGKVAKYVTDFGTDDILGEENIICLPHLGAST
                     PESEENCAVMACDQVKEYLENGNIINSVNYPAISLARTNADDTRFCVAHKNVPELLKK
                     VLTQISGNVENMLSKSRGDYAYTIIDVAGAGADDLAKIAAVDGVIRVRAI"
     gene            complement(1246..2331)
                     /gene="serC"
                     /locus_tag="PLS64_07390"
     CDS             complement(1246..2331)
                     /gene="serC"
                     /locus_tag="PLS64_07390"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015533539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HRL43681.1"
                     /translation="MARVYNFSAGPSMLPESVLKKAAAEMLDYEGSGQSVMEMSHRSK
                     IYGTIIEGAEALLREVMNIPDNYKVLFLQGGASTQFAAIPMNLMTKSGKADYVITGQW
                     AKKAHAEAARYGQANVVASSADKTFTYIPKLDPSTFTKDADYFYICMNNTIYGTVYHE
                     LPDTGDVPLVADISSCILSRPIDVSKFGVLYAGAQKNMAPAGLTVVIVREDLIGNAMD
                     ITPTMLNWKTHADNGSMFNTPPCYTIYIAKLVLEWIKNEVGGLDKMYEINKKKADLLY
                     GFLDSSELFKGTVVPEDRSLMNVPFVTGDADLDAKFVKEATENGLVNIKGHRTVGGMR
                     ASIYNAMPYEGVEKLVEFMKKFEAENK"
     gene            complement(2484..3419)
                     /gene="pyrF"
                     /locus_tag="PLS64_07395"
     CDS             complement(2484..3419)
                     /gene="pyrF"
                     /locus_tag="PLS64_07395"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRL43682.1"
                     /translation="MAFDRLIENIVKMQNPTVAGLDPKLDYVPESIKTKCFEKYGKTL
                     EGAAAALFEFNKALIDALYDIVPAVKPQAAYYEMYGWQGVKALCDTIAYAKSKGMFVI
                     TDGKRNDIGTTMEAYATAHLGHTDVAGESIDAFGADALTVNGYLGTDGIKPLAKICDS
                     DDKGIFVLVKTSNPSSGELQDMKLETNESVYEHMGKMCEGWGEDLMGKHGYSAVGAVV
                     GATYPEQLGEMRAKLPHTFFLVPGYGAQGGGADDVKNAFDENGLGAIINSSRGIMCAW
                     KKQGLTEDDFAVAARKEAERMRDEIVGCIGKIKLG"
     gene            complement(3668..4648)
                     /locus_tag="PLS64_07400"
     CDS             complement(3668..4648)
                     /locus_tag="PLS64_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HRL43683.1"
                     /translation="MAQKGDLLSVRQDIRVVDATIRDGGLCNDFRFDDKFIKALYNAN
                     VKAGVDYMEFGYKASKEIFNEEDFGKWKFCNDADIRKIVGDNKTPLKIAVMADVGRTD
                     FKKDIIAKKDSPIDLIRVATYINAIPAAVEMIEDAAKKGYETTINIMAVSKARTEDIT
                     AALETLGKTPVNAFYIVDSYGALYPEESRRLADLYCSIADKYGKSVGIHAHNNQQLAF
                     ANTIEAMTQGVSYLDATVDGMGRGAGNCALELLLGFLKNPKYQVAPILKIIEEYTHKF
                     KAEGVKWGYDIPYLLTGQYNTHPRPAIQFVKEDRQDYYKFSNELYDIINS"
BASE COUNT         1281 a         1108 c          984 g         1388 t
ORIGIN      
        1 aaatcagatt gcacgaactc tgattacgcc gtcaactgct gcgatttttg caaggtcatc
       61 tgcaccggcg cctgcaacat caataattgt gtaagcatag tcgccacggc tctttgaaag
      121 catattttca acattgccgc tgatctgtgt gagaaccttc ttgagaagct cgggaacatt
      181 cttgtgagca acgcagaaac gggtatcgtc agcatttgtt cttgcaagtg aaattgcagg
      241 atagtttacg gagttgatga tgttaccgtt ctcgaggtac tccttaacct gatcacaagc
      301 cataacagca caattttctt cactttcggg agttgatgca ccgaggtgtg ggaggcagat
      361 aatattctct tcaccgagga tatcgtctgt tccgaagtct gtaacatact tagcaacctt
      421 gccgctctta acagcctcaa gaactgctgt tgaattaaca agaccgtcac ggctgaagtt
      481 gagaatacga acaccgtctt tcatcttggc aatcatatcg gcatcaacca tatctcttgt
      541 gtcgtcagta agcggaacat ggattgtgag gtagtcgcaa tttaccataa catcttctgc
      601 gctcttcatg agcttaactg taggagcaag cttgagagcg ttgccaacag acataaacgg
      661 gtcaaagccg attacattca taccgagagc aacagcggca tttgcaacaa gaataccgat
      721 tgctccaaga ccgataacac cgagggtctt gcccttgatt tcgggaccga cgaactgact
      781 cttgcccttt tcaaccatct taccgacagc tgcaccgttg cccttgagag tctttgccca
      841 ttcataaccc tcaataacct tacgatttga aataagaagt cctgcgataa caagctcctt
      901 aacagcgttt gcgttagcac ccggtgtatt gaacacaaca ataccctgct cgctgcactt
      961 atccttcgga atgttgtttg tgcctgcgcc tgctcttgca attgcaagaa ggttatcggc
     1021 aaattccata tcgtgcattg cggcacttct tacaagaact gcgtcagggt tctgaacatc
     1081 gtcaccgtat ttatagttat caccgagacg gcttgtgccg atagcggcga ttttgtttaa
     1141 tgtaagaatg ttatacatca aaatcaatcc tttttaattt tttattttaa gaatttacaa
     1201 taacaggcga acagcgttcg cccgttatat cataatattc aataattact tgttttcagc
     1261 ctcgaacttc ttcataaact caacgagttt ttcaacgccc tcataaggca tagcgttgta
     1321 gattgaagct ctcataccgc cgactgtgcg atgacccttg atattaacaa gaccgttttc
     1381 agtagcctct ttaacgaatt tagcgtcaag gtcagcgtca cctgtaacga acggaacatt
     1441 cataagcgaa cgatcctcgg gaacaacagt acccttgaaa agctctgaag aatcgaggaa
     1501 accgtagaga aggtcagcct tcttcttgtt gatttcatac atcttatcaa gaccgccaac
     1561 ctcgttctta atccactcaa gaacaagctt tgcaatgtag attgtgtagc aaggcggtgt
     1621 gttgaacatt gaaccgttgt cagcgtgagt tttccagtta agcattgtag gagtgatatc
     1681 catagcgttg ccgataagat cctcacgaac gataacaacc gtaagacctg ccggagccat
     1741 gttcttctgt gcgcctgcat agagaacgcc gaactttgaa acatcaatcg gtcttgaaag
     1801 aatacaggaa gaaatatctg caacgagcgg aacatcacct gtgtcgggaa gctcgtgata
     1861 aacagtgccg taaattgtgt tgttcatgca aatgtagaaa tagtcagcgt cctttgtgaa
     1921 tgttgaagga tcaagctttg gaatgtatgt gaaggtcttg tcagccgatg aagcaacaac
     1981 attagcctga ccgtaacggg cagcctctgc atgagccttt ttagcccact gacctgtgat
     2041 aacataatca gccttaccgc tctttgtcat aaggttcata gggatagccg caaactgtgt
     2101 tgacgcaccg ccctgaagga aaagaacctt gtagttgtca ggaatgttca taacctctct
     2161 caaaagagcc tcggcaccct cgatgattgt accgtaaatt tttgagcggt gtgacatttc
     2221 cataacgctc tgaccactgc cttcgtagtc gagcatttct gccgcagctt ttttaagcac
     2281 agactcaggc agcattgaag gacctgccga aaaattatat actcttgcca tttctgatat
     2341 ctcccttttt aaagaaaatt taaaaatata gttgtaaaaa taaatacact taaattatac
     2401 gcttttagca cgctaaagtc aataaattca tagcactaaa gcataaaaaa cgaaaaattt
     2461 cgtgattctg ttgtgcaaat gaatcagcca agcttaattt tgccgataca accaacgatt
     2521 tcatccctca tacgctcagc ctctttacga gcggcaacgg caaaatcatc ttctgtaagt
     2581 ccctgctttt tccaagcaca cataatgccg cggcttgagt tgatgattgc accaagaccg
     2641 ttttcgtcaa atgcgttttt aacatcatcg gcaccgccgc cctgagcacc gtaaccggga
     2701 acaaggaaga atgtgtgcgg aagttttgct ctcatttcac cgagctgttc gggatatgtt
     2761 gcacccacaa ctgcacctac tgccgaatag ccatgcttac ccataaggtc ttcgccccag
     2821 ccttcgcaca tcttgcccat atgctcataa acgctctcat ttgtttcaag cttcatatcc
     2881 tgaagttcgc ccgagcttgg gtttgaagtt tttacaagaa caaaaatgcc cttgtcgtcc
     2941 gaatcgcaaa tttttgcaag cggcttgata ccgtctgttc cgaggtagcc gtttaccgtg
     3001 agtgcgtctg caccgaatgc gtcaatactt tcgcctgcaa catctgtatg accgaggtgt
     3061 gccgtagcat atgcctccat agttgtgccg atatcgttac gcttgccgtc tgtgataacg
     3121 aacataccct ttgactttgc ataagcgatt gtgtcacaaa gagccttaac accctgccag
     3181 ccgtacattt cataatatgc agcctgtggc tttacggcag gaacaatatc ataaagtgcg
     3241 tcaataagcg ccttgttgaa ttcaaaaagt gcagcagccg caccctcaag cgtcttgccg
     3301 tacttttcaa agcattttgt tttgattgat tcgggaacat agtcaagctt agggtcaaga
     3361 cctgcaaccg taggattctg catttttaca atattttcga taagtctgtc aaatgccata
     3421 gtgattctcc tcattttttg taatgtaaaa acaatttgtt cgttcatatt ttgatttaaa
     3481 caatgtcatt gcttaaccgt agcggcggtc aatgaccgcc aatcgttttg gctgtgaaac
     3541 caaaacgaat aaacaacaaa tgtattgtta tatcgtggtt aattacttta attcaaatcg
     3601 tttgtatggt cgaaccgatt ttgcaatcgg ttcgattggc gttcaatgaa cgccgctaca
     3661 aatttaatta gctgttgata atatcataaa gttcatttga gaacttgtaa taatcctgtc
     3721 tgtcctcttt tacgaactga attgcaggac ggggatgtgt gttgtactga cctgtgagaa
     3781 ggtacggaat atcgtaaccc cacttaacgc cctcagcctt aaacttgtgg gtgtactcct
     3841 caatgatttt gagaatagga gcaacctgat acttcgggtt cttgaggaaa ccgagaagaa
     3901 gttcaagcgc acagttgcct gcgcctctgc ccatgccgtc aacggttgcg tcaaggtagc
     3961 tgacgccctg agtcatagcc tcaattgtgt tggcaaaagc aagctgctgg ttgttgtgag
     4021 cgtgaatacc gaccgacttg ccgtacttgt cggcaattga gcagtaaagg tctgcaagtc
     4081 gtcttgactc ctcgggatag agtgcgccgt agctgtcaac aatgtagaaa gcgtttacgg
     4141 gagttttgcc gagagtttca agagccgctg taatatcctc ggttcttgcc tttgaaacag
     4201 ccataatatt gattgttgtt tcgtagccct tctttgcagc gtcctcaatc atttcaacag
     4261 ccgcaggaat tgcgttgatg tatgttgcaa ctctgatgag gtcaatcggg ctgtccttct
     4321 ttgcgataat atccttctta aaatctgttc tgccgacatc agccataaca gcgattttaa
     4381 gaggagtctt gttgtcgcct acaatttttc tgatatctgc atcgttacag aatttccact
     4441 taccgaaatc ttcttcgtta aagatttcct ttgatgcctt gtagccgaat tccatataat
     4501 caacaccggc tttaacattt gcattgtaaa gagccttgat aaatttgtcg tcaaatctga
     4561 aatcgttaca aagaccgccg tcacggattg ttgcgtctac tactctgata tcctgtctta
     4621 ccgacaggag gtcgcctttc tgtgccataa ttttactcct ttatgttaaa taaattttct
     4681 gtttatttac cgacagtcat tgactgccat tttaaccgat tggcaaacac ggttcgccgc
     4741 tacagtatat cattaacttt g
//
LOCUS       DAOMVE010000129         4601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5331_length_4601_cov_2.923669, whole genome shotgun sequence.
ACCESSION   DAOMVE010000129 DAOMVE010000000
VERSION     DAOMVE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5331_length_4601_cov_2.923669"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    307..316
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            373..585
                     /locus_tag="PLS64_07405"
     CDS             373..585
                     /locus_tag="PLS64_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43684.1"
                     /translation="MVAFEDFDGQMHRHMIHLPNEDVDKILNTGIMVTVQFIMLKAER
                     AMQGKLLLILTERKCVKPPMAKQNAL"
     gene            731..1009
                     /locus_tag="PLS64_07410"
     CDS             731..1009
                     /locus_tag="PLS64_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43685.1"
                     /translation="MYNAYNEKTTDENLLREYKKYLDNGEVTEKESRLWKFVLSNYGT
                     DEFSTKMLENDFQNAAYAAIREFVQKFERLGLLSSTKYGTRIKYKINT"
     gene            complement(1086..1274)
                     /locus_tag="PLS64_07415"
     CDS             complement(1086..1274)
                     /locus_tag="PLS64_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL43686.1"
                     /translation="MSFLRLYLKYDGSVQRVAQETFVHRNTINYQLAKIKKILGNNLK
                     TFEERFKIILAFEVRDVL"
     gene            complement(1725..1928)
                     /locus_tag="PLS64_07420"
                     /pseudo
     CDS             complement(1725..1928)
                     /locus_tag="PLS64_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557237.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     gene            complement(2190..3089)
                     /locus_tag="PLS64_07425"
     CDS             complement(2190..3089)
                     /locus_tag="PLS64_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43687.1"
                     /translation="MGNSKSVKKGVAVVTAVIVAVVMVCVSVVCNLFSTKTESSNQTY
                     LDDAKAQLTEYSDNYNYTKFEFMNSLFEKANVIASVVTENTSADTNAALVKNLGINSF
                     VMTDADGKIIASSDDKKVGTNFLDDETTKQFKANLKGMKVKSISEPTAVEGEDGVYNL
                     MACVARTEGGIVIIDTNTSDYSAVIGADIAKSCKGDTIIVKNGEVVSTNMNLGDNGLK
                     SAGITDEMIRSENFNVTIDGTTYSLSSMPSGEYTIISGQAATESGFNMIYGVVIPCAA
                     GVVMIIISAIILSLGTTKKKENE"
     assembly_gap    3139..3148
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3304..3957
                     /locus_tag="PLS64_07430"
     CDS             3304..3957
                     /locus_tag="PLS64_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL43688.1"
                     /translation="MADILIVEDEKNISNLIEMALSRHGHKTDCVFDGESCADVLEKK
                     HFDLILLDVMLPKISGFELMDYIKGFDTPVIFITAKSDISDRVYGLNLGADDYITKPF
                     NISELQARVDAVLRRYNKAKTKLSFKDIVIDTNSRIVTKNGVQIDLTLKEYEILLLFV
                     RNKNIALYREMIYERVWNEPYMGDTRTVDLHIQRLRKKLDLKDNIQSVYKVGYRLVE"
     gene            3975..>4601
                     /locus_tag="PLS64_07435"
     CDS             3975..>4601
                     /locus_tag="PLS64_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43689.1"
                     /translation="MKFSWKIFFITFLIIITSFGAGGFLLINTVFTNTLNNRIDSAKK
                     NNNYITTALSVYADNNQPTYGNLEYLRVSAIGFAKQIAGNSDSKIKIGSINELSFSAE
                     NEFAHGMSVNSRKSRVVTENKKYYIQTISKIQLVTKSCYIETVDNITSVYSDRDMYCT
                     IYQFILIGVALFASILLVIFSKLLTRPLVKLKDASKEVAEGNFNMRVKE"
BASE COUNT         1439 a          876 c          847 g         1419 t
ORIGIN      
        1 agagtagtgg cagtaagtaa gcccgaaata atcttctttt ttatcttcat aactttctcc
       61 tttataattt ttttgcgttt ctatatatta gacagttgaa atgtcgaaaa ggttcatttt
      121 ttacgaaaat aaaatattat taaaaaggag tacatagaca agctacgcat ccgaaaaaca
      181 catagccctt ttgtttggct tctttatttt agcatacatt atgtgaaaat tcaatatttt
      241 ataaaagctt tcaaaaacta aaattccgat tactaaaaaa tttaattatc ttgcttcgta
      301 ttctttnnnn nnnnnnataa cgataagatg attagagcat tagtggagca ttgaaagaag
      361 acaaatgacc ttgtggtggc ttttgaagat tttgacggtc aaatgcacag gcatatgatt
      421 catttgccaa atgaagatgt tgacaaaatt cttaatactg ggattatggt gacggttcaa
      481 tttattatgt tgaaagccga aagagctatg cagggcaaat tactcttgat attaacggaa
      541 agaaaatgcg taaaaccgcc tatggcaaaa cagaacgcat tgtaaaaaac aaattgcttg
      601 agtaccgtat tcaagcaaaa ttgaaaaaac tcacaaagca tattataacg catacaccgc
      661 aattgaagaa aacaaaagat acagcggtaa aattgacatt acaccgtttt taatctattt
      721 tgccgataat gtgtacaatg catataacga gaaaacaact gacgaaaatc ttctccgtga
      781 atacaaaaaa tatcttgata acggtgaagt caccgaaaaa gaatcccggc tttggaaatt
      841 tgtactgtca aattacggta cagacgaatt ttctaccaaa atgcttgaaa atgactttca
      901 aaatgccgca tatgccgcaa taagagaatt tgtacagaaa tttgaaagac ttggtttgct
      961 ttcatcgaca aaatacggta caagaatcaa atataaaata aacacataaa aaatcccccg
     1021 acagcggttt ccgttaggct gtcgggggat tttcagtatt aacatcgtga cggggacgga
     1081 ggcggtcaaa gcacatcacg aacttcaaag gctaatataa ttttgaatcg ttcttcaaag
     1141 gttttgaggt tattgccgag aatctttttg atttttgcaa gctgataatt tatggtgttg
     1201 cggtgaacaa aggtttcctg agcaaccctc tgaacacttc cgtcatactt taggtaaagt
     1261 cttaaaaaac tcatataatc cgtgccgttg tcacggtcat acacttcaag ctttttgaga
     1321 ttttcgttat agatactttc aagtagcgaa atatcgtcaa ccgcaagaat cagcttttta
     1381 acttcaagtc ggtcatagaa cataggcgtc atatctgcac ggacggcaag ccttaacatt
     1441 cttaaaacaa tctggtatgt tttcgggagt gatttcagct tgttatttgt tttgctcact
     1501 gccacataca gcttgttgac gcttatatac ctctcctttt ccgactgaat tttttcggct
     1561 atgctttcaa tttcatcatc ggaaaagtca ttgaggatat agaacatcat gttgtcaacc
     1621 ttaaagccgc cccagtgctt tttgtaggaa taaattgttc gttcaaatgc cgattcaagc
     1681 tttcttgaag aaatatggtc agccatatcg gctttcacac cgatgattgc atcaccgatt
     1741 gctttgtttc cgtaaccgag attgaggttt acaagctgtg atgacatatc tgtatatctg
     1801 ttaccgtcaa aatcgtaaaa gtaaattccg tctgcctttg caacgggaat cggattgaga
     1861 ttaccctgct tgctccatga ctggaggaca tatttcttat ggttttcaac aattttctca
     1921 ggtgtcataa taatttcccc tttttaagat accaaaaaag ataaacagaa aaggagcctc
     1981 cttttcagaa gctcctttgc tttgcgtctt ttttgtttat gccattatta tatcaccaaa
     2041 atatggttat gcaatatttt tcggcacaat attctttgtg ctaaagcaca atgttttcgg
     2101 agatatttct tgaatttatg accttttcgt cattttacat acaaatttcg tgaaatgtcc
     2161 acttcttgtt tgtatgctgt aattaacaat tactcgtttt cctttttctt tgttgtgcca
     2221 aggctgagta taattgcgga aatgataatc attacaacac ctgcggcaca cggaattacg
     2281 acaccgtaaa tcatattgaa accgctttct gtcgcagcct gtccgctgat gattgtatat
     2341 tcacctgacg gcatactgct gagtgaatat gttgtgccgt caattgtaac attgaaattt
     2401 tccgaacgaa tcatttcgtc tgtaatacct gcgcttttaa gaccgttatc gccaaggttc
     2461 atatttgtgc ttacaacttc gccgttctta acgatgattg tatcaccctt gcagcttttt
     2521 gcaatatcag caccgataac agccgaatag tcgcttgtat ttgtatcaat aataacgatg
     2581 ccgccctctg ttcttgcaac gcaagccatg aggttataca caccgtcctc gccctcaacg
     2641 gctgtcggct ctgaaatgct cttgaccttc atacccttga ggttagcctt gaactgcttt
     2701 gttgtttcgt catcaaggaa atttgtgccg acctttttat cgtctgacga tgcgataatc
     2761 ttgccgtcag cgtctgtcat aacaaatgaa ttgatgccga ggttctttac aagtgcggca
     2821 tttgtgtcag ccgaagtatt ttccgtaaca acacttgcaa ttacatttgc cttttcaaaa
     2881 agtgagttca taaactcaaa tttagtgtag ttatagttat cgctgtattc tgtaagctga
     2941 gcctttgcgt catcaagata tgtctgattt gagctttctg tttttgttga aaacaaattg
     3001 caaactaccg aaacgcaaac cataacaaca gcaacaataa ctgccgttac aactgcaacg
     3061 ccttttttta ccgatttaga attgcccatt gtaatcttcc tttcaaaatg ctttgcttgc
     3121 ataaaataat accattatnn nnnnnnnngt acggttcgtg aaataatcaa caactgcgca
     3181 taatttgaca atattctgca ttagccgatt tttggataca gctctgcgaa aaaagataat
     3241 ttgaaaaaaa tgatacatct atgatacaat ggcaatataa actgtgtctt ggaggtgggg
     3301 ttgttggctg acatattgat tgttgaggac gaaaagaata tttcaaatct tattgaaatg
     3361 gctctttctc ggcacgggca caaaaccgat tgtgtgtttg acggcgaaag ctgtgccgat
     3421 gttctggaga aaaagcactt tgaccttatt ctgcttgatg ttatgttgcc gaaaatcagc
     3481 ggttttgagc ttatggacta catcaaaggc tttgacacac ccgtaatttt cattactgca
     3541 aaatcagata tatcggacag ggtttacggg cttaacctgg gggctgacga ttacataaca
     3601 aaaccgttta acatttcgga acttcaggca agagtcgatg cggtactgcg aagatacaac
     3661 aaggcaaaaa caaaactcag ctttaaagac atagtaattg acacaaattc aagaatagtc
     3721 accaaaaacg gagtacaaat tgaccttaca ttaaaggaat acgaaatact tctgcttttt
     3781 gtacgcaaca aaaacattgc cctttaccgt gaaatgattt acgaaagggt atggaatgaa
     3841 ccgtatatgg gtgacacaag aaccgtggat ctgcatattc agcggttaag aaaaaagctt
     3901 gatttaaaag acaacattca gtctgtgtat aaagtcggtt accgtcttgt tgaataagcg
     3961 gagggaaagc cctaatgaaa ttttcgtgga aaatattttt tattaccttt cttattataa
     4021 ttacttcctt cggtgcggga ggttttctcc tcatcaacac cgtttttaca aacacgctga
     4081 acaaccgcat tgactcagcc aaaaagaaca acaattacat cacaacggca ctttcggtat
     4141 atgcagacaa caaccaaccc acctacggaa acttggaata tctgcgcgtt tcggcaatcg
     4201 gatttgcaaa acaaattgca ggcaattcgg attctaaaat caaaatcggt tcaataaacg
     4261 agctcagctt ttcggcagaa aatgaatttg cccacggtat gtcggtaaat tccagaaaaa
     4321 gcagagtagt tactgaaaac aaaaaatact atattcagac aataagcaaa attcagcttg
     4381 taaccaaaag ctgttatatt gaaactgttg acaatataac aagcgtgtac agcgaccgtg
     4441 atatgtactg cacaatttat cagttcattc tcataggcgt tgcgttgttt gcatcaattc
     4501 tgcttgtaat cttctcaaag cttttgacaa gaccgcttgt taagttaaag gatgcgtcaa
     4561 aggaagttgc cgagggcaac tttaatatga gagtaaagga a
//
LOCUS       DAOMVE010000130         4582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5363_length_4582_cov_4.032030, whole genome shotgun sequence.
ACCESSION   DAOMVE010000130 DAOMVE010000000
VERSION     DAOMVE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5363_length_4582_cov_4.032030"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..2466)
                     /locus_tag="PLS64_07440"
     CDS             complement(211..2466)
                     /locus_tag="PLS64_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRL43690.1"
                     /translation="MAKIKKLLALILSICVICTVSVMSVSAANTDDDNDTSASSTITV
                     HYYCEEGTPTIYYWNSLPKNISTEYPGVKMTSEGNNYYKYTFNDVTKINMMFVTNGKQ
                     SAELTRNTGEWWYKNKRWSSKNPEDMQEFDRVDMREDTIYFVITTRFYDGDTGNDVHC
                     WDDSQANNPDSDPAWRGDFKGLIDKLDYIKALGFSAIWITPVVTNASGYDYHGYHAMD
                     FSTVDVRYESDGATYQDLIDAAHEKGIKIVQDIVVNHSGNFGEATLAPMFTKEYDSIQ
                     DLASVDCMKVIEGSDFAKTFPNYDDLEPKYQYAARLNIMKDTKELDSGNHDTENYYHH
                     FKDLSWESPTVQTGQIAGDCVDINTENPTVANYLNDVYNNYINMGVDAFRLDTEKHVS
                     RLTLNQYYFPSWLKTGGKNFFIFGEVCTRVSEFWNHNIPAISAPFYTWAESDSQYIDS
                     FTNDQAANINLTLKHYDSNASTANQPTSDNVYLDGLKYHTPDYSKSNGTSVIDFPMHW
                     NFSNASNAFTRACEEDPYYNDSSWNVTYVDSHDYGPDMDSRYDGGTQSWAENLDVLFT
                     FRGIPCLYYGSELEFQKGVPMDVGPNAPLSTTGRAYFGDYLEGDVTATDFGTYSNASG
                     AVASTLEAPLAVHIQQLNRIRRAVPALQKGQYTRSKTYVDGNMAFVRRYTQGATDSLA
                     CVTISGGATFKNLPNGKYIDAVTGDVKYVTDGTLTVSSVGRANMRVYVCCADGFTGID
                     GQIGTNGAYLK"
     gene            complement(2726..3538)
                     /locus_tag="PLS64_07445"
     CDS             complement(2726..3538)
                     /locus_tag="PLS64_07445"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRL43691.1"
                     /translation="MDYKLIALDIDGTLTNSQKEITPRTRYALMEAQKQGKKIILASG
                     RHPVGIMPIAKDLMLDRFGGFIMAFNGGRIINCETGETMVSKLFPLEYLPDIVNVLKD
                     SNITINTYDDRRIISDNKVNDYTYVERDVIKAEMVVVDDFISSVRFDINKILLAGEPD
                     ELDKYQKILADRYDGLLDVYKSAPYFLEIMPFGVTKGSMLPLLLDKLKIKREELAAFG
                     DNYNDMTMIGYAGFGVAMGNAETDVKKIADYICESNDNDGIANTLDKFVLKQ"
     gene            complement(3764..4507)
                     /locus_tag="PLS64_07450"
     CDS             complement(3764..4507)
                     /locus_tag="PLS64_07450"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRL43692.1"
                     /translation="MDKNNDFENAVEVTSVTTEHPKSKNRIKKRHESKLRKTLRIIKN
                     IICWGVLAILVLTVIVFMGSKVNGKTPSLFGYSVLRVSTGSMQPELMIGDVILGKSVD
                     DPTALKVGDIITYRGSGQTEGLLVTHKVVVAPYKTADGTYKLQTKGIANDAPDPEIST
                     DDVLYIMVCKLGFIDTLYNLFFSPWGLLIFIAALLLIFIDELVVIIRILTGHDKSPKD
                     GDINEIIDRLQKEKLQQKAESDNDTKSEE"
BASE COUNT         1252 a          982 c          931 g         1417 t
ORIGIN      
        1 tgcagaagtg tcataatgat ttttaacctt atatgtagaa tttgcataac tttctgtctt
       61 tttattcatc attatgcaac tttcgggatg tttatttata aatgaagtat aattttgacg
      121 gatataaatc tgcaatccgt catttgaggg tttagggcag aaaaatacgg agcaggaaaa
      181 acctgctccg taaaaaataa cattatttta ttacttgaga tatgcgccgt tagtgccgat
      241 ctgaccgtca attccggtga aaccgtctgc acagcaaaca taaactctca tattagccct
      301 accaacagaa gatactgtaa gtgtgccgtc tgtaacatac ttaacatcgc ctgtaactgc
      361 atcaatatac ttgccgttag gaaggttctt gaatgtagca ccgcctgaga tagttacaca
      421 agcaaggctg tctgtagcgc cctgtgtgta acggcgaacg aatgccatat taccgtcaac
      481 ataagtcttg cttcttgtat actgaccctt ctgaagtgca ggaactgctc ttctgattcg
      541 gttgagctgc tgaatatgaa cagcgagagg tgcttcaagt gtgcttgcaa cagcaccgct
      601 tgcattagaa tatgtaccaa agtctgttgc tgttacgtcg ccctcgaggt agtcaccgaa
      661 gtatgcacga cctgttgttg aaagaggtgc gttaggacct acgtccatag gaacaccctt
      721 ctggaactca agctcagaac cgtagtaaag acacggaata ccacggaaag taaagagtac
      781 atcaaggttc tcagcccatg actgtgtacc gccgtcgtaa cggctgtcca tatcaggacc
      841 gtagtcgtga gaatcaacat atgttacatt ccatgatgag tcattgtagt acggatcttc
      901 ttcacaagca cgggtaaatg cgttgcttgc atttgagaag ttccaatgca tcgggaagtc
      961 gattacgctt gtgccgtttg acttgctgta atcaggtgtg tgatacttaa gaccgtcaag
     1021 ataaacatta tcagatgtgg gctgatttgc tgtacttgcg ttgctgtcat agtgcttaag
     1081 tgtaagatta atgtttgcag cctgatcatt tgtaaatgaa tcgatgtact ggctgtctga
     1141 ttcagcccat gtatagaacg gagctgaaat tgcaggaatg ttgtggttcc agaattcact
     1201 tacacgagta caaacctcac caaagatgaa gaagttttta ccgcctgtct tgagccatga
     1261 cgggaagtaa tactggttaa gtgtaagtct tgaaacgtgc ttttcagtat caagtctgaa
     1321 agcgtcaacg cccatgttga tatagttgtt ataaacgtcg ttaaggtaat tagcaactgt
     1381 aggattctct gtgttgatat caacgcagtc accggcaatc tgacctgtct gtacagtcgg
     1441 gctttcccag ctgaggtctt tgaagtggtg atagtagttt tccgtgtcgt gattaccgga
     1501 gtcaagctcc ttagtatcct tcatgatatt aagtcttgcg gcatactgat acttaggctc
     1561 aaggtcatcg tagttcggga atgttttagc aaagtcgctt ccctcaataa ccttcatgca
     1621 atctacagaa gcaagatcct gaattgagtc gtattccttt gtaaacatag gagcaagagt
     1681 tgcctcaccg aagttacctg aatggttaac tacgatatcc tgaacaatct tgatgccctt
     1741 ttcatgagcg gcatcaataa gatcctgata tgtagcgccg tcgctctcat aacgaacatc
     1801 aactgttgag aagtccattg catggtagcc gtggtaatcg tagcctgacg catttgttac
     1861 aacaggagta atccagattg cgctgaatcc gagagccttg atgtagtcaa gcttgtcgat
     1921 aagacctttg aagtcaccac gccatgcggg atcgctgtcg gggttgtttg cctgtgagtc
     1981 gtcccagcaa tgaacatcgt tacctgtgtc accgtcatag aaacgggttg tgattacaaa
     2041 ataaattgtg tcttcacgca tgtcaacacg gtcaaattcc tgcatatctt caggattttt
     2101 gcttgaccat ctcttatttt tgtaccacca ctcgcctgtg tttcttgtga gttccgcaga
     2161 ctgctttccg tttgtaacaa acatcatatt gatttttgtt acatcgttga aagtgtactt
     2221 gtagtagttg tttccctcgg aagtcatctt aacgccgggg tactccgtgc tgatgttttt
     2281 cggaagactg ttccaataat agattgtggg cgtgccttcc tcacagtagt aatgtactgt
     2341 gattgtgctt gatgccgaag tgtcgttatc gtcatctgta ttagccgcac ttacggacat
     2401 aacagatacc gtgcaaatta cgcaaatcga caaaatcaaa gcaagcaatt ttttgatttt
     2461 tgccatagat tttcctcctt ttgttatact ttgtacaaca taacatacaa ttatattata
     2521 atttagacag cctcatatat ttttctttta aagaattatt acaactttga tgccgccaaa
     2581 gattaagccc tcaatttggc tattatatac aaaaaggagc gacagattct gttttttcaa
     2641 ctcttttaaa gggttaagtc aaatctgacg ctccgttaaa ttatatttta tattttcaag
     2701 cttatattag gttcaaactt tgcgactatt gctttaaaac gaatttatca agtgtgtttg
     2761 caataccgtc gttatcgttt gattcgcaga tgtaatcggc aatcttcttt acatcggttt
     2821 cggcattacc cattgcaacg ccgaagcctg cataacctat cattgtcata tcgttgtagt
     2881 tgtcgccgaa agcggcaagc tcttcccttt taatcttgag tttatcaagc aaaagcggaa
     2941 gcattgagcc ttttgtaaca ccaaacggca taatctcaag aaaataaggt gcgcttttat
     3001 atacatcgag caagccgtca taacggtctg caagaatttt ctgatactta tcaagctcat
     3061 cgggttcgcc tgcaagaaga attttgttga tatcaaatct tacagatgag ataaaatcgt
     3121 caacaacaac catttcagcc ttgataacgt ccctttcaac ataggtatag tcattaacct
     3181 tattatccga gatgattcgc ctgtcatcgt atgtgttgat tgtaatgttt gaatccttca
     3241 aaacattcac aatgtcggga agatattcaa gcggaaacag cttgctcacc atagtttcgc
     3301 ctgtttcgca attgataatc ctgccgccgt tgaaagccat aataaaaccg ccgaaacggt
     3361 caagcataag gtcttttgct atcggcataa ttcctacggg atgtctgcct gacgcaagaa
     3421 taattttctt gccctgcttc tgcgcctcca taagagcata tcttgtacgg ggagtaatct
     3481 ccttttggga gtttgtcagc gtaccgtcaa tgtcaagcgc tattaatttg taatccatga
     3541 aaccaccctt aacatatcaa tttctaaaca caatcagtat gttacagtca aatttgaccg
     3601 tataaaatat accacaaatc gcccaaaaat gcaactaaaa agccgatttt agcccatata
     3661 aaagctattt tcaccatttt tccgctttgt ctttttcgcc aacaaaaaac ggacgaaaag
     3721 agaattttcc tttcgtccga aattaaatat attcagcttt tatttattct tccgattttg
     3781 tatcgttgtc ggattcagcc ttttgttgca atttttcctt ttgcaaacgg tctataattt
     3841 cgttgatgtc gccgtccttc ggtgatttgt catgtcccgt aagtatgcgg attataacga
     3901 caagctcatc aatgaatatc agcagcagcg ccgcaataaa aatcagcagt ccccacggtg
     3961 agaagaaaag gttgtacaat gtgtcaatga atccaagctt gcacaccata atataaagca
     4021 catcatcggt tgaaatttca gggtcgggag catcgtttgc aatgcccttt gtctgtagct
     4081 tatatgtgcc gtcagctgtt ttatacggtg caactacaac cttatgagtc acgagaagtc
     4141 cctcggtctg cccactgccg cgataggtga taatatcgcc gaccttgagg gcggtcggat
     4201 cgtcaacgct ctttccgaga ataacatcgc cgatcataag ctcgggctgc atactgcccg
     4261 ttgaaactcg aagcacggaa tatccgaaaa gcgaaggtgt ttttccgttc acctttgaac
     4321 ccataaacac aattacggtc agcacaagta ttgcaagcac tccccagcaa attatatttt
     4381 taattatccg cagggttttg cgaagctttg attcatgcct tttctttatt ctattttttg
     4441 attttgggtg ttcggttgtt acagatgtca cttcaaccgc attttcaaaa tcgttatttt
     4501 tatccataaa ttcctccacg gcaagttgcc gttctcagtt cgtgtcagta ggggcggtca
     4561 ttggccgccc gccttgatga aa
//
LOCUS       DAOMVE010000131         4520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5475_length_4520_cov_3.735274, whole genome shotgun sequence.
ACCESSION   DAOMVE010000131 DAOMVE010000000
VERSION     DAOMVE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5475_length_4520_cov_3.735274"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..3797
                     /gene="nifJ"
                     /locus_tag="PLS64_07455"
     CDS             237..3797
                     /gene="nifJ"
                     /locus_tag="PLS64_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRL43693.1"
                     /translation="MARKMKTMDGNSAVAHVSYAFTDVAAIYPITPSSVMADLTDKFS
                     AQGAKNLFGRQVQVTEMQSEGGASGAVHGSLAAGALTTTYTASQGLLLMIPNMYKMAG
                     ELLPGVIHVSARALTSHALSIFGDHSDIYACRQTGYAMLCSNNPQECMDLAAVAHLAA
                     IEGRVPFLHFFDGFRTSHEIQKIEEWDYADLADMLNWDAVEAFRRRALNPEHPVTRGT
                     AQNDDIFFQAREACNKYYDAVPEVVVKYMDKVNAKIGTDYKPFNYYGAEDAEHVIVAM
                     GSVCDCTEEVVDYLNAAGEKVGLLKVHLYRPFVADYMLRELPKTVKTISVLDRTKEPG
                     SIGEPLYLDVLAAINGSDFAGVKVYTGRYGLGSKDTTPGDIIAVYRNAESETPKRRFT
                     IGIVDDVTNLSLPIVENPDTTPKGTHSCKFWGLGADGTVGANKNSIKIIGDNTDMYAQ
                     GYFAYDSKKSGGLTVSHLRFGKTPIKSTYYISKADFVACHNPSYVDKYDIVDDLKEGG
                     SFLLNCPWDTEELSERLPGKMKKILAERHINFYTIDGIKIGKEIGLGGRINTVLQSAF
                     FKIADIIPADKAKELMKAAAKKSYMKKGQAIVDMNYAAIDRGMEDLKKVEIPADWANA
                     VDNSVADKAEGNGALVEYVNEILKPVNAYKGNKLPVSTFMDHVDGTAPNGSAAYEKRG
                     IAVDVPEWNPENCIQCNRCAIVCPHAVIRPVAMTADELAKAPEGTKSLPMMGLKDLNF
                     VMTVSTLDCTGCGACAQVCPGKKGAKALTMQPIDSQRPKQAVFDYALTLEDKPEVHEK
                     FKFTTVKGSQFKQPLLEFSGACAGCGETPYAKLVTQLFGDRMFIANATGCSSIWGASA
                     PATPYTKNKKGYGPAWQNSLFEDNAEFGLGMALGQKAIRNRLIEYVEGIQKDTDSADL
                     KTACQNYLDTVTDSTSSRPATDALIAELEKNTEDAKVGELVKKTLVDKDELAKKSMWI
                     FGGDGWAYDIGFGGLDHVIASGENVNILVFDTEVYSNTGGQASKATPVGSVAQFAAAG
                     KAVKKKDLAAIAMSYGYVYVAQCAMGADNNQVLKAMVEAESYNGPSLVICYAPCINHG
                     IKGGMGIAQLEEKKAVEAGYWNIFRYDPRLADEGKNPFMLDGKAPSASYRDFIMGEVR
                     YNSLTRSFPERAEKLFEKAEKVAADKYAHLEKLASLPDAE"
BASE COUNT         1251 a          992 c         1039 g         1238 t
ORIGIN      
        1 ttgacaggaa caagtaaata taaaaaactt ttttgtaaaa aacgaaacaa aaatatatat
       61 cctgctttga atttcttgtg taaaacactt tacaaatcgg tgtttaagtg ataaaattta
      121 agatgataca aagtatccgt accgcttgtt gatttacggt gcgtaattaa cgggtatcga
      181 aaatttatgc ggcatcattt cctttgccgc tttgattaaa aaggaggacg attccattgg
      241 caagaaaaat gaaaactatg gatggtaaca gcgcagtagc tcatgtttct tatgcgttta
      301 ccgatgttgc ggctatctat cctattacac cttcttccgt aatggctgac cttaccgaca
      361 agttctcggc acagggtgcc aagaaccttt tcggcagaca ggttcaggta acggaaatgc
      421 agtcggaggg cggcgcatca ggtgctgttc acggctcact cgcagcaggt gctttgacca
      481 caacttacac agcatcacag ggcctgctcc ttatgattcc gaatatgtac aagatggcag
      541 gcgagctcct tccgggcgtt attcatgtat cagcccgtgc gctcacaagc cacgcacttt
      601 ctatttttgg tgaccactca gatatctacg cttgccgtca gacaggttat gcaatgcttt
      661 gttcaaacaa tcctcaggaa tgtatggacc ttgccgctgt tgctcacctt gcagctattg
      721 aaggcagagt tcctttcctt catttctttg acggtttcag aacttctcac gaaattcaga
      781 agattgagga gtgggattac gcagacctcg ccgatatgct taactgggat gcagttgagg
      841 ctttccgtcg tcgtgctctt aaccctgagc atcctgtaac aagaggtact gctcagaacg
      901 acgatatctt cttccaggca agagaagctt gtaacaagta ttatgacgca gttcccgaag
      961 ttgttgttaa gtacatggac aaggttaatg ctaaaatcgg cactgactac aagccgttca
     1021 actactacgg tgcagaggat gcagagcatg ttatcgttgc tatgggttct gtttgtgact
     1081 gtacggaaga agttgttgat tacctcaatg ccgcaggcga aaaggtcggt cttcttaaag
     1141 ttcaccttta cagacctttc gttgccgact atatgctcag agagcttcct aagactgtta
     1201 agacaatctc tgttcttgac agaacaaagg aacccggttc aatcggcgaa cctctttatc
     1261 ttgatgttct cgccgctatc aacggttcgg actttgcagg tgtaaaggtt tacacaggca
     1321 gatacggtct tggctcaaag gatacaacac ccggtgacat cattgctgtt tacagaaacg
     1381 ctgaaagcga aacacctaag agaagattta caatcggcat tgttgatgat gttacaaacc
     1441 tctcacttcc gattgttgaa aatcccgata caactccgaa gggtacacac agctgtaagt
     1501 tctggggtct tggtgctgac ggtactgtcg gtgctaacaa gaactcaatc aagattatcg
     1561 gcgataacac cgatatgtac gctcagggct actttgcata tgactcaaag aagtcgggcg
     1621 gtcttacagt ttctcacctt cgtttcggta aaactcctat taagtcaaca tactacattt
     1681 caaaggcaga ctttgttgcc tgtcacaatc cgtcttatgt tgacaagtac gatattgttg
     1741 atgaccttaa agaaggcggt tcattccttc ttaactgccc atgggataca gaagagcttt
     1801 ccgagcgtct cccgggtaag atgaagaaga ttctcgctga aagacacatc aacttctaca
     1861 caattgacgg tatcaagatc ggtaaggaaa tcggtcttgg cggtcgtatc aacacagttc
     1921 ttcagtcagc attcttcaag attgcagata tcattcctgc cgacaaggct aaggagctta
     1981 tgaaggctgc cgctaagaag tcatacatga agaaaggtca ggctatcgta gatatgaact
     2041 acgccgctat cgaccgtggt atggaagacc tcaaaaaggt tgaaattcct gctgattggg
     2101 ctaacgctgt tgataattca gttgccgaca aggcagaggg taacggcgct ctcgttgagt
     2161 atgtaaacga aatcctcaag cctgtaaatg catataaggg taacaagctc cctgtttcaa
     2221 catttatgga tcatgttgac ggtactgctc ctaacggttc tgccgcttat gaaaagagag
     2281 gtatcgcagt tgatgttccc gaatggaatc ctgaaaactg tattcagtgt aacagatgtg
     2341 caatcgtatg tcctcacgct gtaatccgtc ctgttgccat gacagccgat gagcttgcaa
     2401 aagctcccga gggtacaaag agcctcccga tgatgggact caaggacctc aactttgtaa
     2461 tgacagtttc aacactcgac tgtacgggtt gcggcgcttg tgctcaggtt tgtccgggta
     2521 agaagggcgc taaggctctc acaatgcagc caatcgactc acagcgtcct aagcaggcag
     2581 tatttgacta cgctctcaca cttgaagata agcctgaagt tcacgagaag ttcaagttca
     2641 ctacagttaa gggcagtcag ttcaaacagc cgttgcttga attctcaggc gcttgcgcag
     2701 gctgcggtga aacaccgtat gcaaagcttg ttacacagct tttcggtgac agaatgttta
     2761 tcgctaacgc aacaggctgt tcatcaattt ggggtgcatc tgctcctgca acaccttata
     2821 caaagaataa gaagggttac ggtcctgctt ggcagaactc actctttgaa gataacgcag
     2881 agtttggtct tggtatggct ctcggtcaga aggctatccg caacagactt atcgagtatg
     2941 ttgagggtat tcagaaggat acagacagcg ctgacctcaa gacagcttgc cagaactatc
     3001 tcgacacagt aacagattca acttcaagcc gtcctgcaac agacgctctt atcgctgaac
     3061 tcgaaaagaa cacagaggat gcaaaggttg gcgaacttgt taagaaaaca ctcgttgaca
     3121 aggacgaact cgctaagaag tcaatgtgga tcttcggcgg tgacggttgg gcttacgata
     3181 tcggtttcgg cggtcttgac catgtaatcg catcaggcga aaatgttaat atcctcgtat
     3241 tcgatactga ggtttattct aacacaggcg gtcaggcttc aaaggctact cctgtaggtt
     3301 cggttgcaca gtttgccgcc gcaggtaagg ctgttaagaa gaaagacctt gccgctattg
     3361 caatgagcta cggctatgtt tatgttgcac agtgcgctat gggtgctgac aacaatcagg
     3421 ttctcaaggc tatggttgag gctgaaagct acaacggtcc gtcactcgta atctgctacg
     3481 ctccgtgtat caaccacggt atcaagggtg gcatgggcat tgctcagctt gaggagaaaa
     3541 aggctgttga ggcaggttac tggaacatct tccgttacga tcctcgtctt gcagacgaag
     3601 gaaagaatcc gttcatgctt gacggtaaag ctccttctgc atcatatcgt gacttcatca
     3661 tgggcgaagt tcgttataac tcactcactc gttcattccc cgaaagagca gaaaaactct
     3721 ttgaaaaggc tgaaaaggtt gctgctgata agtatgctca ccttgaaaag cttgcaagcc
     3781 ttcccgatgc cgaataattt tggctaaagc ttgtacaaag tgaatatgtg aataaataaa
     3841 aaattctccc gaatatggca tttgccgtgt tcgggatttt tttaattgaa aattgaaaat
     3901 ggaaagtgga aaattgtagc ggcgttcagt gaacgccaat cgaaccgatt gcaaaatcgg
     3961 ttcgaccaca ggcaacggca gtattttttc gtttaaataa taatttataa atgtaaaccc
     4021 gtaggggcgg tcatcggccg cccgccttga tgaaatattt tgtttaatca catatgtttt
     4081 gatgtgatta taattgtgta ttgaaccttg cacgcattgc gtgcaatcgt atgcgttgca
     4141 tacgaacggg cgaacacggt tccccctaaa acatacattg caaaaataat tggaatttta
     4201 tacttggtca aaatatgtat gttgctgtgt ggttgatttg gctttgcaac caaatcaaat
     4261 ggcggtcact gaccgccgct actgtatgta caaacaatta ctatgttatg ctcgggcaac
     4321 atttgtaatt tactattaaa accgtctgca cgaacacacg aacataaaaa atgcggtggc
     4381 acattgccaa ccgcattttt attttacatt ttcaattttc cactttcaat ttaaaagctc
     4441 cgctcggaga acagtggcgc accatgttta ttgcaccgtt accgtaatcg ttacgcttga
     4501 tgttctttta agcacatcgg
//
LOCUS       DAOMVE010000132         4486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5536_length_4486_cov_3.302415, whole genome shotgun sequence.
ACCESSION   DAOMVE010000132 DAOMVE010000000
VERSION     DAOMVE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5536_length_4486_cov_3.302415"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1749)
                     /locus_tag="PLS64_07460"
     CDS             complement(<1..1749)
                     /locus_tag="PLS64_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRL43694.1"
                     /translation="MIKKFTSGNPIDTQAVVEKFNALPIAEFPLGGKFENGNFVFEFD
                     MADSDIVYGLGEAPRGINKRGWVYNSFCSDDPFHTETKSSLYAAHNFLMLSGSKTFGI
                     FIDFPSKIRWDIGYTTANKTVITIDGTDFDIYYIDGTKPLEIVKEFRKSIGTSYVPPF
                     WAFGYQQSRWSYHNAEAVDAVVDGYNKAGIPLDCVYLDIDYMERYKDFTVDDEKFPNF
                     KDYVSKKREEGIHLIPIIDAGVKKENGYDIYEEGRKNGYFCKNEDGTDFEGGVWPGIV
                     GFPDFLRPDVRKWFGSKYKILTDMGIDGFWNDMNEPAIFYSVKGLNKALDKAASLKGQ
                     NLDLSKFFELKDTFAGLSNSPEDYSSIYHTVDGKQVCHDQVHNIYGANMTKAAGEYFA
                     EEFGKEKILMFSRASYVGAHRSSGIWFGDNHSWWSHILLDLKMLPSANMCGFLYCGAD
                     LGGFNENATRDLVLRFLALGVFTPLMRNHAALGTRNQECYSFENSDDFKDIVEVRYRL
                     IPYLYRTFKKASKENDMIFRPLSFDYPDDKIARECETQLMLGDECMICPVYEQNVGGR
                     YVYLPENMTFVKLSGTS"
     gene            complement(1956..2660)
                     /locus_tag="PLS64_07465"
     CDS             complement(1956..2660)
                     /locus_tag="PLS64_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HRL43695.1"
                     /translation="MDFLRALITAPVSIAVLFLLSKFIGNKQMSSLNLFDYINGITIG
                     SIAAEMATAEMSDFWACLAALIVYVIIVMLCSWLSQKSTMLRRFLTGKSIVIFDRGKL
                     YKKNFSVAKIDINEFLTRMREKGYFSLDDVETVFLEQNGDLSVLPKDKKRPATPDDLK
                     IIVHQTRPESVVITDGKILEKNLAESGFNLEWLKKQLDNNRKRLKDVFLGVCDDTGNL
                     KIYQDGKSESKNDIFD"
     gene            complement(2717..3628)
                     /locus_tag="PLS64_07470"
     CDS             complement(2717..3628)
                     /locus_tag="PLS64_07470"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HRL43696.1"
                     /translation="MADLSVKVCGVEFNNPLIAASGTFGFGMEYSELYPLEKLGGISC
                     KGITLTRRDGNPVPRVAETPSGILNAVGLQNPGVDVFINEDLPWLKKQNTVIIANIAG
                     NTPEDYCAMAEKLSETDVDMIELNISCPNVKSGGVQFGTSCESVGAITAAVKKYCKKP
                     LIVKLSPNVTDIASIAQSAEANGADAISMINTLTGMRIDINSRRPIIRNNTGGMSGPA
                     IFPVAVRMVWQVANAVKIPIIGMGGISSWQDAIEMMMAGATALQIGTVLFTDPYAPIK
                     IADGMNEWLDKNGIKSVSEITGTVKPW"
     gene            complement(3621..4382)
                     /locus_tag="PLS64_07475"
     CDS             complement(3621..4382)
                     /locus_tag="PLS64_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HRL43697.1"
                     /translation="MKKYDVQKFELLSNEQIADGIFDMRVKNDELAPLAKCGQFAHVY
                     VPSKTLRRPISVCDSENGVLRLVYQVKGEGTKIMSEMKKGESVDILAPLGNGFKIEKG
                     KRYCLIGGGIGVPPMLYTAKQCENPLVITGFRNKDLIILQDDFKKAGAELVLTTDDGS
                     AGIHGFVTDVLKEKISEVDEVCACGPTPMLKAIADVCKEAGKPCQISLEERMACGMGA
                     CLVCAVKVRKNGEEIMQHVCKNGPVFNAEEVVFNG"
BASE COUNT         1199 a         1044 c          912 g         1331 t
ORIGIN      
        1 gcttgtaccg ctgagtttta caaaagtcat gttctcgggg agataaacat atcttccgcc
       61 tacattctgc tcatatacag ggcaaatcat gcactcgtca ccgagcataa gctgtgtttc
      121 acattcacgg gcaattttat catcgggata atcaaacgag agtggtctga aaatcatatc
      181 attttctttg cttgcctttt tgaatgttct gtaaaggtac ggaataagtc tgtaacgaac
      241 ctcaacaata tccttgaaat catcgctgtt ttcaaagctg tagcactcct gatttcttgt
      301 gccgagagcg gcatggttac gcataagcgg agtaaacaca ccgagtgcaa ggaaacgaag
      361 aacaaggtca cgggttgcgt tttcgttaaa gcctccgaga tctgcaccgc agtacagaaa
      421 gccgcacata tttgccgacg ggagcatttt gaggtcaaga agaatgtgcg accaccaaga
      481 gtggttatca ccgaaccaga taccgcttga acggtgagcg cctacatatg acgcacggga
      541 gaacataaga attttttctt taccgaactc ctccgcaaaa tattcgccgg cggcctttgt
      601 catattagca ccgtaaatgt tgtgaacctg atcatggcaa acctgtttgc cgtcaactgt
      661 atggtaaatg cttgaataat cctcgggact gtttgaaaga cctgcgaatg tgtccttgag
      721 ctcaaagaat tttgaaaggt caaggttctg ccctttgaga gaagccgcct tatcaagtgc
      781 tttgttaagt ccttttaccg aatagaaaat tgcaggctcg ttcatatcgt tccagaagcc
      841 gtcaattccc atatctgtga gaattttata ttttgagccg aaccactttc ttacatccgg
      901 tcggagaaaa tcgggaaagc caacgatacc gggccataca ccgccctcaa aatccgtgcc
      961 gtcctcgttt ttgcagaagt atccgttctt tctgccctct tcatagatat cgtagccgtt
     1021 ctcttttttt acgcctgcgt caataatcgg aatgaggtgg attccttctt cccttttctt
     1081 tgaaacatag tccttgaaat tcggaaattt ttcatcatca acagtaaagt ccttgtaacg
     1141 ctccatatag tcgatgtcga gatatacgca gtcaagcgga atccctgcct tgttgtaacc
     1201 gtcaacaaca gcgtcaacag cttcggcatt gtgatagctc caacggctct gctgatagcc
     1261 gaatgcccaa aacggcggaa catagcttgt gccgattgat tttctgaact cttttacgat
     1321 ttcaagcggc tttgtgccgt caatgtaata gatatcaaaa tccgtgccgt caattgtgat
     1381 aacggttttg tttgcggttg tgtagccgat gtcccaacgg atttttgacg ggaagtcgat
     1441 aaaaatacca aaagtttttg aaccgctgag cataaggaaa ttgtgagcgg catagaggga
     1501 tgactttgtt tcggtgtgga acgggtcatc actgcaaaag ctgttataca cccaaccacg
     1561 cttgttgatt cctctcggag cttcgccgag accgtagacg atgtcgctgt ctgccatatc
     1621 aaattcaaag acaaaatttc cgttttcaaa cttgccgccg agcggaaatt ccgcaatagg
     1681 cagagcgtta aatttttcaa caacagcctg cgtatcaatc gggttgcccg atgtaaactt
     1741 tttaatcatt aaagcacttc cttctttatt aaaattttcc gattttttca aagcgttctg
     1801 caaaatcttg ccgacacgca cttgcaattt tagtataaca ttaattttga ttattgcaat
     1861 gataaaattt atttttgtat tattttattt ttcgcacatt aaaaaagcac ctttacgagc
     1921 ctgtaaaggt gcaaatttta ttcttttacg ctttatcagt caaaaatatc attcttgctt
     1981 tcagactttc cgtcttggta aatcttgagg tttcccgtat catcacacac accgagaaaa
     2041 acatctttga gccgtttgcg gttgttgtca agctgttttt tgagccattc aagattaaag
     2101 cccgattcgg caagattttt ctcgagaatt tttccgtccg taataaccac ggactcgggt
     2161 cgggtttggt gaacaatgat ttttaaatca tccggagttg cagggcgctt tttgtccttt
     2221 ggcagaaccg acaaatcgcc gttctgttca agaaaaaccg tttcgacatc atcaagactg
     2281 aaatatccct tttcacgcat tcgtgtaaga aattcgttta tatcaatttt tgcaaccgaa
     2341 aaattctttt tgtacaactt tcctctgtcg aaaatcacaa tagacttgcc tgttaaaaat
     2401 cttcgtaaca ttgttgattt ttgcgaaagc cacgaacaaa gcataacaat tattacataa
     2461 acaatgagtg ccgcaagaca cgcccaaaag tctgacattt cggctgttgc catctccgct
     2521 gcgattgaac cgattgtgat tccgttgatg tagtcaaaaa gattaaggct tgacatctgc
     2581 ttgttgccga tgaactttga cagcaggaac aatactgcaa ttgaaacggg cgccgttatt
     2641 aatgcacgca aaaagtccat aatatcccct ccgatagggt tattatggac tttaaattat
     2701 tttttattcc gcaaaattac caaggcttaa ctgtgcctgt gatttccgaa acgctcttga
     2761 ttccgttttt gtcgagccat tcgttcattc cgtcggcaat cttaataggc gcatacggat
     2821 ccgtaaaaag gacagtaccg atttgaagtg cggttgcgcc tgccatcatc atctcgattg
     2881 catcctgcca gcttgagata ccgcccatac cgataatcgg aattttaact gcgtttgcaa
     2941 cctgccaaac cattcttact gcaacaggga aaattgcagg gccgctcatt ccgcctgtgt
     3001 tgttgcggat aatcggtctg cgtgagttga tgtcgatacg catacctgtg agcgtgttaa
     3061 tcattgaaat tgcgtctgca ccgtttgcct ctgccgactg tgcgattgat gcaatgtcgg
     3121 taacatttgg gctgagcttt acgataagcg gttttttgca atactttttg acagccgcag
     3181 taattgcacc tacggattca cagcttgtgc cgaactgcac tccgccgctc ttgacattag
     3241 ggcatgagat gttgagctca atcatatcta catcggtttc gctgagtttt tcagccattg
     3301 cacaataatc ttcgggagtg ttgccggcaa tgtttgcaat gataaccgta ttctgctttt
     3361 tgagccacgg caaatcctca ttgataaaaa catcaacacc ggggttttga agtcctactg
     3421 cattcaaaat gccggaagga gtttcggcta ctctcggaac agggttgccg tcacggcggg
     3481 taagtgtgat acccttgcat gaaataccgc cgagcttttc aaggggataa agctctgaat
     3541 attccatacc gaaaccgaat gttcccgatg cggcaatgag aggattgtta aattccactc
     3601 cgcaaacttt tacagataaa tcagccattg aaaactacct cctcagcatt aaatacgggg
     3661 ccgtttttgc agacatgctg cataatttct tcaccgtttt ttcttacctt tactgcgcac
     3721 acaaggcatg cacccattcc gcaggccatt ctctcctcaa gagaaatctg acacggcttt
     3781 cctgcctctt tgcaaacatc ggcaatagcc ttgagcatcg gggtaggacc gcatgcacaa
     3841 acctcgtcaa cctctgagat tttttctttg agaacatctg ttacaaagcc gtgaattccc
     3901 gcacttccgt catctgttgt cagcacaagc tctgcaccgg ctttcttgaa atcatcctga
     3961 agaataataa ggtctttgtt tctgaagcct gtgataacaa gcggattttc acactgcttt
     4021 gctgtgtaga gcatcggagg aacgcctata ccgccgccga taaggcagta acgcttgccc
     4081 ttttcaattt taaagccgtt gccgagagga gcgagaatat caacgctttc gccctttttc
     4141 atctcggaca taatttttgt tccctcaccc tttacctgat aaacaaggcg gagaacaccg
     4201 ttttcgctgt cacaaaccga aataggtctg cgcagagttt ttgaaggcac ataaacatgg
     4261 gcaaactgtc cgcactttgc aagcggagca agctcgtcat tttttaccct catatcaaaa
     4321 atgccgtctg caatctgttc gtttgacaaa agttcaaact tctgaacatc atactttttc
     4381 ataatatcat tcctttggtt agctttattt atataaaatc aattcataac aaatcaactt
     4441 tcaatcggag tgtccctgca ctgcggacaa aagtgaacat ctcatc
//
LOCUS       DAOMVE010000133         4484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5542_length_4484_cov_2.815308, whole genome shotgun sequence.
ACCESSION   DAOMVE010000133 DAOMVE010000000
VERSION     DAOMVE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5542_length_4484_cov_2.815308"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..477
                     /locus_tag="PLS64_07480"
     CDS             232..477
                     /locus_tag="PLS64_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006876286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HRL43698.1"
                     /translation="MLDKTMIFSLGEEKDDQIKAGLTIVYDALRQKGYNPINQIVGYI
                     LSEDPTYITTYNNARNIISKIDRDDLLEALVKSYLNV"
     gene            494..799
                     /locus_tag="PLS64_07485"
     CDS             494..799
                     /locus_tag="PLS64_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43699.1"
                     /translation="MADTKKTEKKFSTYKGRPLVRCNDEIYYGSMEEPYVIRMQIKSK
                     KDVNGTEIADKVTVQLMATDPYLSPRKQLVKSSEKKGLFLAMDIADIWLERALANKM"
     assembly_gap    1045..1077
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1149..1421)
                     /locus_tag="PLS64_07490"
     CDS             complement(1149..1421)
                     /locus_tag="PLS64_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HRL43700.1"
                     /translation="MATTNLNVRVDENLKKTADTLLNELGLNMSTAINIYLKQIVREN
                     GIPFEIKLDKPNAETLQAMQDVREGKNLHGPFNSLSELMEDLNADD"
     gene            complement(1608..>2888)
                     /locus_tag="PLS64_07495"
     CDS             complement(1608..>2888)
                     /locus_tag="PLS64_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-transporting ATPase"
                     /protein_id="HRL43701.1"
                     /translation="VGNGVGAKNGILFKTAVSLETAGRIDTVVLDKTGTVTKGEPAVT
                     DIVTAENISETELLKTAVALEKSSEHPLAKAIVVYANDRHIIGDAVTEFNAVTGNGLT
                     AKIGSESVYGGNYRYISQFAEISSDEKAKAEQLAEEGKTPLYFCKGNKFLGIIAVADI
                     IKEDGKQAVNELKNMGVRVVMLTGDNEKTAKAVAKNADIDEVVAGVLPDGKEKNVKEL
                     KKYGKVAMVGDGINDAPALTSADLGIAIGAGTDVAIDAADVVLVKSRLADVPATIRLG
                     RATLRNIHENLFWAFIYNVIGIPLAMGAFINLFGWQLNPMFGAAAMSLSSFCVVTNAL
                     RLNFVKVHSSKHDRKIKHKNKKENKTMKKTMKIEGMMCPHCEATVKKTLEAIDGVESA
                     EVSHEKGSAVLTMSKNVEDSVLKRAVEDAGYKVN"
     assembly_gap    2889..2898
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2899..4110)
                     /locus_tag="PLS64_07500"
     CDS             complement(<2899..4110)
                     /locus_tag="PLS64_07500"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRL43702.1"
                     /translation="MTQYNVTGMSCAACVARVEKAVSKIDGVNSCSVSLLTNSMNVDG
                     SADSSAIIKAVQNAGYGASLKGKEKKSPDAEDSLKDTETPKIRKRLIASLIVLAVLMY
                     FSMGHMMWGWPLPSFFDNNHIAMGLVQLILSGIVMVINQKFFVSGFKGIIRLAPNMDT
                     LVALGSGTAFVYSTIALFMMTDAQVRGDSAAVMSYMHEFYFESAAMILALITVGKMLE
                     AHSKGKTTDALKGLMKLAPKTATIVVDGVENTVPIEQVKKGDIFAVRPGESIPVDGVV
                     IDGESAVNESALTGESVPVDKLVGDTVSAATINQSGYIRCEATRIGEDTTLSQIIKMV
                     SDASATKAPIAKIADKVSGVFVPAVITIAVITAIVWLLLGQSVGFSLARAISVLVISC
                     PCALGLATPVAI"
     gene            complement(4119..4424)
                     /locus_tag="PLS64_07505"
     CDS             complement(4119..4424)
                     /locus_tag="PLS64_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002851018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRL43703.1"
                     /translation="MENNKCEHCAKHKHRTEKEYKSLVNRLNRIEGQIRGIKGMLEKD
                     AYCPDILVQVAAANAALNAFNKELLANHIRTCVADDIRAGNDETIDELVGVLQKLMK"
BASE COUNT         1275 a          984 c          825 g         1357 t
ORIGIN      
        1 tgaaaacgga gtaaagctca gaggctaaaa attatggggg cggttttttc cgtccccttt
       61 ttgtgacata atctgacaag cataagataa aattaaaaaa tattactgct gttttatcac
      121 acaaagcaat gtttttgaca ttgaaataac ataaattagg catatttatt aaaaagtgct
      181 tgaaatttac tttgaaatat ggtatagtag cagaatagga ggtgtttcag tatgttggat
      241 aaaacaatga ttttttctct tggtgaggaa aaagatgacc agataaaagc agggcttaca
      301 atcgtttatg atgccctccg ccaaaaagga tataacccta ttaatcagat tgtgggatat
      361 attttaagcg aggatccgac ctatattaca acatataata acgctcgcaa catcatcagc
      421 aagatcgacc gtgacgacct acttgaagca ctggtaaaat cgtatcttaa tgtttaagaa
      481 aggatgatgc cttgtggcag ataccaaaaa gaccgaaaag aaattttcga cttacaaggg
      541 cagaccgctc gttcgttgca atgacgagat ttactacggc agtatggaag aaccgtatgt
      601 tataagaatg caaatcaaaa gcaaaaagga tgtcaacgga actgagattg ccgataaggt
      661 aacagttcag ctaatggcta cagaccctta tctttcaccg agaaaacagc ttgttaagtc
      721 aagcgagaaa aagggattgt tccttgctat ggatattgca gacatttggc ttgaaagagc
      781 ccttgcaaac aaaatgtaat ttaacattga atttacggag taatcggttt tggttactcc
      841 gttttttctt atgtagaaca acatctttgt tgcatttata aaaccagtct tatcacaaac
      901 tttttaccgt tacatgaaaa gtgtatagtg gatgttccca tacaaatgat tacaaaactt
      961 tgaaatttta ttatacataa aaagcactct gcatttctac aaagtgcctt aatttaaaat
     1021 attaagttca gaataaatcg ccacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaac
     1081 tccttttgaa agtagatagt aagagttttt atatcctttt aaaatccttt ttaaacaggg
     1141 tatgatactt aatcgtcagc attcaagtcc tccataagct cgcttaaaga attaaaagga
     1201 ccgtgaagat ttttgccctc tctgacatcc tgcatagcct gcaaggtttc tgcatttggc
     1261 ttatcaagtt tgatttcaaa cggtatgccg ttctccctga caatttgttt gagatagata
     1321 ttgattgctg ttgacatatt caaaccaagt tcgttaagca atgtgtcagc tgttttttta
     1381 agattttcat caactctgac attcagattt gttgttgcca taaaatcaac tcctttatat
     1441 ctgtatatgt ataataccac atacaaatta caatgtcaag caattgtaat tacgctgtca
     1501 ttattttgca caattttaca tacaataaaa caaaggtcgg gaaacagagc atttcatttt
     1561 acacactctc ttcccgacct taattttcaa ttttacattt taaattttca atttactttg
     1621 tatcctgcgt cctctactgc tcttttaaga acggaatctt caacattttt tgacattgta
     1681 agaacggctg agcctttctc gtggcttact tctgccgatt caacaccgtc aattgcctca
     1741 agagttttct ttactgttgc ctcgcaatgc ggacacatca taccttcgat tttcattgtt
     1801 tttttcatgg ttttattctc ctttttattt ttatgtttta tttttctgtc atgctttgag
     1861 ctgtgaacct taacaaaatt aagccttaat gcatttgtta caacacaaaa gcttgaaagg
     1921 ctcattgccg ccgcaccgaa catcggattt aactgccagc cgaaaagatt gataaatgca
     1981 cccatggcaa gcggaatacc gattacattg taaataaatg cccagaaaag attttcgtga
     2041 atgttacgga gtgttgctct gccgagtctg attgttgcag gaacatctgc aaggcggctc
     2101 tttacgagta caacatctgc cgcatcaatt gcaacatccg ttcccgctcc gattgcaata
     2161 cccaagtctg cgcttgtgag ggcaggcgca tcgttaatgc cgtcaccgac cattgcaacc
     2221 ttgccgtatt ttttcagctc ttttacattc ttttccttac cgtcaggaag aactcctgca
     2281 acaacttcat caatgtctgc gttttttgca acggcttttg cggttttttc gttatcgccc
     2341 gtaagcatta caacacgcac acccatattt ttgagttcat taacagcttg tttgccgtcc
     2401 tcttttatta tgtctgcaac agcaataata ccgagaaatt tatttccttt acagaaatac
     2461 agcggagttt tgccctcttc cgcaagctgt tcagcctttg ctttttcgtc tgatgaaatt
     2521 tcagcaaact gtgaaatata tctgtaattg ccgccgtaaa cgctttcaga gccgattttt
     2581 gcggtaagtc cgttacccgt tacggcattg aactcagtca cggcatcgcc gataatatgt
     2641 ctgtcatttg cataaactac aattgccttt gcaagcggat gttcgcttga tttttcaaga
     2701 gcaacggctg ttttcaaaag ctcggtttcc gagatatttt cggctgtaac gatatcggtc
     2761 acagcaggtt cacccttagt aaccgtgcct gttttgtcaa gaacaaccgt gtcaattctg
     2821 cctgccgttt caagcgaaac tgcggtttta aagaggattc cgttctttgc accgacaccg
     2881 ttgccgacnn nnnnnnnnaa tcgcaacagg agttgcaagt cccaatgcac acggacaact
     2941 gattacaaga acggaaatgg ctcttgcaag cgaaaatcct acactttgac cgagcaacag
     3001 ccacacgatt gcagtaatca cggcaattgt gattaccgca ggaacaaaca cacccgatac
     3061 cttgtcggca atttttgcga taggtgcttt ggtggctgac gcatcgctga ccattttaat
     3121 aatctgcgac agcgttgtgt cctcaccgat tctcgtagcc tcacagcgga tatatcccga
     3181 ctgattgatt gttgcggctg aaaccgtatc gccgacaagc ttgtcaacag gaacgctttc
     3241 acccgtcaga gcggattcat tgacggcact ttctccgtca ataacaactc cgtcaacagg
     3301 aatactctca cccggacgaa ctgcgaatat atcgcctttt ttcacctgct ctatcggaac
     3361 agtattttca acaccgtcaa ctacaatcgt agcagtcttt ggtgcaagtt tcatcaaacc
     3421 tttgagagca tcggtagttt tgccctttga gtgagcctca agcattttac cgactgtgat
     3481 gagtgccaaa atcattgcgg ctgattcaaa atagaactcg tgcatatacg acataaccgc
     3541 cgccgagtca cctctcacct gtgcatcggt catcataaac aacgcaattg tgctgtaaac
     3601 aaaggctgtg cccgaaccaa gtgcaacaag cgtgtccata ttgggagcaa ggcggataat
     3661 ccctttgaat ccgctgacaa aaaatttctg attaatcacc atcactatgc ctgacaaaat
     3721 cagctgaaca agtcccattg caatatggtt gttgtcaaaa aatgacggca aaggccatcc
     3781 ccacatcata tgtcccattg aaaaatacat caacactgca aggacaatga gcgatgcgat
     3841 aagtcttttt ctgattttgg gtgtttcggt atctttgaga gaatcctctg catcgggcga
     3901 ttttttctct ttgcctttca aagacgcacc atagccggca ttctgcacgg cttttataat
     3961 tgcagacgaa tcagctgaac cgtcaacatt cattgaattt gtgagcagac tgacggaaca
     4021 ggaatttact ccgtcaatct ttgataccgc cttttcaaca cgggcaacac aggcggcaca
     4081 gctcataccc gttacattgt attgagtcat agtttctcct acttcataag cttttgcaaa
     4141 acaccgacaa gctcgtcaat tgtttcatcg ttacctgccc ttatgtcatc tgcaacacag
     4201 gtgcggatat gattagcaag caactccttg ttaaaagcgt tgagtgccgc attggcggcg
     4261 gcaacctgta ccagaatatc gggacagtag gcatcttttt caagcatacc ctttatcccc
     4321 cgtatctgac cttcaatacg attaagtcgg tttacaagcg acttgtattc tttttcggta
     4381 cggtgcttgt gttttgcaca atgttcacac ttattattct ccataatatc aacctcataa
     4441 tttcaaaatg ggatatatta ttcaaagccc ttttaagata cccc
//
LOCUS       DAOMVE010000134         4441 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5623_length_4441_cov_2.164615, whole genome shotgun sequence.
ACCESSION   DAOMVE010000134 DAOMVE010000000
VERSION     DAOMVE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5623_length_4441_cov_2.164615"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLS64_07510"
     CDS             complement(<1..336)
                     /locus_tag="PLS64_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43704.1"
                     /translation="MAEHFNSATRHTPIDIDTALFRELSKYTKEFALRIAAALKSYRS
                     SVFSDLSELESQLDFYGGAAQFISSVRARGMKMCRPKFLPSEKRVTRLKNVFDLNFYR
                     QLVTQNPQEI"
     gene            complement(293..1030)
                     /locus_tag="PLS64_07515"
     CDS             complement(293..1030)
                     /locus_tag="PLS64_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43705.1"
                     /translation="MKITDYVDLFYPSAEAEEYAKKRQKKYENYPQDFMVNLETDNIA
                     KLIAPMNFAVATSKMKEFTDDIETLDYRLDCLEDFMNVPELNGRFRQLINELAGNRID
                     FSDVDCVNSFMQIKTHMDSLEEFLGCIENINRFFAKYNHAIKSVAVKRLAQFFEKLPK
                     SENIFEIAYSISQLKDTFSKTIRSVKIGVNFDSAMNPDSAGLLEIGYDKIYPKGNILE
                     KLVFKSFAGREQFIGGAFQLGNKAHSH"
     gene            complement(1048..1719)
                     /locus_tag="PLS64_07520"
     CDS             complement(1048..1719)
                     /locus_tag="PLS64_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43706.1"
                     /translation="MVKLNNIVKIYNSKKANEFKALHGVSAEIRDGELIAIIGKSGAG
                     KSTLLHILACIDNYQEGEYYIDDVLVKDLSESQYAKIRNEKIGMVMQDFALVEDFTAL
                     ENVMIPLNFSKKKVSDKKEKALAALKSVGIEELAKKLCSKLSGGQKQRVAIARAIVNE
                     PSMILADEPTGALDTKTSAEIMELFKSLNKQGRTVVIVTHDPKIAEQCGRVIEISDGN
                     IVTAK"
     gene            complement(1722..3050)
                     /locus_tag="PLS64_07525"
     CDS             complement(1722..3050)
                     /locus_tag="PLS64_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43707.1"
                     /translation="MRIKLYGRVIGIFLKKHSFINVLISLQLIAVFVIGIIMTSIIDE
                     KASSYEAVKPLLNGEGLYCGGYFLKDNENGGLLENTDEIKTQLKDVDFISSVNFASLQ
                     TGVDTQTNEVKTAYALVYDDYSANLFRPTIEKGSWITDSKQSDDIPQAVITRNYQGYN
                     VNDIAEFETENGTLKVRIIGVIGDNEKYLGANSEYESNEPSYQLMLSTHFNCLNDQLK
                     NKGFTNEEIALKLESLGYSADSIVYNEPVLFFTDKEWNKTDLDSVMSDSLFIKYNRNI
                     SDDEKLYNRKYINENLSVLSFAIQFSQLNNNSQTIVYRELYTLMPIMCAIYLLVLIAS
                     VSVNAINCKNNIRNEAILYLGGAKRKQLLTINVIYNGLICIVSLIISIAGSLIFYKAG
                     LFSKTVITLGWKQITICLIVCAVYILVSCIIPCIILTPKALKKAIILSEE"
     gene            complement(3047..4192)
                     /locus_tag="PLS64_07530"
     CDS             complement(3047..4192)
                     /locus_tag="PLS64_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43708.1"
                     /translation="MYTLKNLRNMMKNHTGMLVLLVISMFVSFGVLFFGVGLYYQYSK
                     NIEDGEIDSYAVGFSINDIITKKNFCEFVKNMPNKLMGDVSYITCFSSTHVSGIDEEI
                     PVAFYLQYSEGKFEYSDKVFQPMIDDLVIKDGSFFTQEQYSNGEKKAVVMGSGNVNQP
                     TSAPEYTNSVTAFGQSYDVIGTINPANSSYFFYSIYVPFSSVPDDTIMNDGVYLALNN
                     KITKSEYDDFTAYISEFFKDKITLYEPAFDLASNTSYYITVVLISVIIAILSALNISI
                     LYNYIIISRTRQLTIMRICGGLTSNLSISTANEIILIMLPVSIISALCYDKFILTVLS
                     AKFPLMKAAYNPYVYAEIILIYSAISYLLNLFLLHHNLGKKIKEGLK"
BASE COUNT         1291 a          922 c          793 g         1435 t
ORIGIN      
        1 aatttcctgc ggattttgcg taacaagctg cctgtagaag ttgaggtcaa agacgttttt
       61 cagccttgta acacgcttct cagaggggag aaatttcggt cggcacattt tcattcccct
      121 tgccctaaca cttgatataa actgtgccgc tccgccatag aagtcaagct gtgattccag
      181 ctccgaaagg tctgaaaaaa ccgaggaacg atagctttta agtgctgcgg caatccgcag
      241 ggcgaactcc tttgtgtatt tgctcagttc acggaaaagg gcagtgtcta tatcaatggg
      301 agtgtgcctt gttgccgagt tgaaatgctc cgccaataaa ctgctctctg cctgcaaaac
      361 ttttgaacac aagcttttca agaatatttc ccttgggata aattttgtca tatccgattt
      421 caagcagtcc tgcactgtcg ggattcatag ccgaatcaaa attaacaccg atttttacgc
      481 tccgtatggt ttttgaaaag gtatctttca gctgtgaaat gctgtatgct atttcgaaaa
      541 tgttctcgct ttttggcagt ttttcaaaaa actgtgcaag cctttttacc gctaccgact
      601 taattgcgtg attatacttt gcaaagaacc tgttgatgtt ttcaatgcag ccgagaaatt
      661 cttcaaggct gtccatatgg gtcttaatct gcataaacga gttgacacaa tcaacatctg
      721 aaaagtcaat tctgtttcct gcgagttcat ttatcagctg tcgaaatctg ccgttaagtt
      781 cggggacatt cataaagtcc tcaagacaat ccagcctgta atcaagggtt tcaatgtcat
      841 ctgtaaactc tttcatttta gatgttgcga cagcaaaatt cataggtgca ataagttttg
      901 ctatgttatc cgtttcaaga ttgaccataa aatcctgcgg ataattttca tattttttct
      961 gccgcttttt tgcatactcc tcagcctccg ccgacggata gaacagatct acataatcgg
     1021 taattttcat tgttttcact ccacaattta ttttgctgta acaatattcc cgtcactaat
     1081 ctcaataacc ctgccgcatt gctccgcaat cttcgggtcg tgggtaacta taacaaccgt
     1141 cctgccctgt ttgttcaggg acttgaaaag ctccataatc tcagcggaag tctttgtgtc
     1201 cagcgctccc gtcggttcgt ccgccaaaat cattgacggt tcattgacta tcgctctggc
     1261 aattgccaca cgctgcttct gtccgcccga aagtttgctg catagctttt tggcaagctc
     1321 ctcaatgcct accgatttca gtgccgcaag ggctttttct ttcttgtcac tgactttctt
     1381 tttggaaaaa ttcagcggta tcatcacgtt ttccagtgcc gtgaaatcct caaccagtgc
     1441 aaaatcctgc ataaccatcc cgattttctc gtttctgatt tttgcatact gactttcgga
     1501 aaggtctttc acaagcacat catcaatgta gtattcgccc tcctgatagt tgtcaatgca
     1561 ggctaaaata tgcagaagcg ttgattttcc tgcacccgat tttccgataa tcgcaataag
     1621 ctcaccgtcc cgaatttctg ccgaaacacc gtggagagcc ttgaattcgt tggctttctt
     1681 tgaattatat atttttacta tgttattaag ttttaccatt actattcctc cgataatatt
     1741 attgcttttt tcaaagcctt tggtgtgagg ataatgcagg gaatgataca ggataccaag
     1801 atatatacag cacacacaat caggcaaatg gtgatctgtt tccagcccaa tgtaattact
     1861 gttttgctga acaatcctgc cttgtagaaa atcaacgatc ctgctattga aattatcaaa
     1921 gacacaatgc aaatcagccc attgtaaatt acattgattg taagcaactg ttttcttttt
     1981 gcaccaccca aatacagtat ggcttcgttt cttatattat ttttgcagtt aatcgcatta
     2041 acgctcactg acgcaatcag tacaagcagg tatattgcac acattattgg catcaatgtg
     2101 tacaactctc tataaacaat ggtctgactg ttgttgttca actgagaaaa ctgtatcgca
     2161 aatgataata cagaaagatt ctcgttgata tattttctgt tgtaaagctt ttcatcatcg
     2221 gaaatattcc ggttgtactt tataaaaagg gagtctgaca ttactgaatc caaatcggtt
     2281 ttattccact ccttatctgt aaaaaacaat acaggttcat tatatacaat gctgtccgct
     2341 gaatacccaa gagattcgag tttcaaagca atctcctcgt ttgtaaagcc tttgttttta
     2401 agctggtcat tcagacagtt gaaatgtgta gaaagcatta actgatagga tggttcattt
     2461 gattcatatt ccgagtttgc tccaaggtat ttttcattat caccaattac accgattatt
     2521 ctgaccttca acgttccatt ttcagtttca aattcagcaa tatcattaac attatagcct
     2581 tgataatttc gggtaattac agcctgcggt atatcatcgg attgcttgga atcagttatc
     2641 cacgaacctt tttctattgt cgggcgaaaa agatttgcag aataatcatc atatacaagt
     2701 gcatatgcgg tcttaacctc gttagtttgt gtatcaacgc ctgtttgcag ggacgcaaag
     2761 tttacagacg agataaaatc aacatctttc agctgagttt tgatttcatc tgtgttttcc
     2821 aaaagtccgc cattttcatt atctttcaaa aaatatccac cacaatacaa tccctctcca
     2881 ttaagcagcg gtttgacagc ctcatatgag gaagcctttt cgtctattat cgatgtcata
     2941 attattccga taacaaaaac agcaatcagc tggaggctta ttaaaacatt gataaacgag
     3001 tgctttttca ggaagatacc gataaccctg ccgtataact taattctcat ttcaatccct
     3061 ccttaatttt tttgccgaga ttatgatgaa gcaaaaacag attaagaaga tacgatattg
     3121 cagagtaaat caaaattatt tctgcgtaaa catatggatt atatgcagct ttcataagcg
     3181 gaaatttagc tgaaagtaca gtaagaataa atttatcata gcacaaagct gaaataatgc
     3241 ttacaggaag cataataaga ataatctcat ttgcggttga tatgctcaga tttgaggtta
     3301 accctccgca gatacgcata atggtcagtt gtcttgttct gctgataatg atgtaattat
     3361 acagaatgga aatgttcaat gccgaaagaa tggcaatgat taccgaaatc agtacaactg
     3421 ttatgtagta ggaagtgttt gatgccaagt cgaatgccgg ttcataaaga gtgattttat
     3481 ctttgaaaaa ttcgctgata tatgcggtaa aatcatcata ttcagattta gtgattttat
     3541 tgttgagtgc aagataaacc ccgtcgttca taattgtatc gtcggggact gagctgaacg
     3601 gaacatagat actgtaaaaa aaatagctgc tgttagcagg gtttattgtg ccgataacat
     3661 catagctctg accgaatgct gtaaccgaat ttgtatattc gggtgctgaa gtcggctgat
     3721 ttacatttcc gctgcccatt acaacagcct ttttttctcc gtttgaatat tgctcctgcg
     3781 tgaaaaaact gccgtcctta attacaaggt catcaatcat tggctgaaat actttatcgg
     3841 aatattcaaa ttttccttca gaatactgaa gatagaatgc gacaggtatt tcctcatcaa
     3901 ttccgcttac atgcgtggac gaaaaacagg taatatatga aacatcaccc ataagcttat
     3961 tcggcatatt cttcacaaac tcacaaaaat ttttttttgt aataatgtca tttatggaaa
     4021 aaccgacagc atagctgtca atttcgccgt cctcaatatt tttgctgtac tgatagtaca
     4081 atccaacacc gaaaaacaaa accccgaatg aaacaaacat agaaatcaca agtagaacca
     4141 gcataccggt atgatttttc atcatatttc taagattttt cagcgtgtac attctttcct
     4201 ccttcaaacc cattaaggca atgcattatc tgatgttatt cagataatgc attgcctttg
     4261 taaaatgttc aattgatttt tattaaaatt tctttaatgc atatttaatt cggagcaaag
     4321 cccgatgtaa gcgtagcttt tcatcggctg cgacgaagca agcttgccgc aggagcttta
     4381 gctcctcagg gttatatatt agtaatcaga ctgaatatcc ttaaaaactc tattatcttt
     4441 c
//
LOCUS       DAOMVE010000135         4420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5659_length_4420_cov_5.210538, whole genome shotgun sequence.
ACCESSION   DAOMVE010000135 DAOMVE010000000
VERSION     DAOMVE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5659_length_4420_cov_5.210538"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1211)
                     /locus_tag="PLS64_07535"
     CDS             complement(57..1211)
                     /locus_tag="PLS64_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL43709.1"
                     /translation="MFKSENNQITIEQVRKLDEEYTLVDIRDEISFEYGHIDGAKNIP
                     LAKIKEDNSLLPKDKLVVLCCKSGQISDELAENLRDDGFNAVNLEGGYYSWLRSQFEN
                     EDYATDVEKSIRKKFSKTIWSRFTAAIIEYKLVEPNDKIAVCISGGKDSMLMAKLFQE
                     LKRHNKFPFELVFLVMDPGYSVENRDVIESNARRLNIPITVFETDIFNSVYNVDKYPC
                     YLCARMRRGYLYKKAKQLGCNKIALGHHYDDVIETILMGMLYGGQVQTMMPKLHSTNY
                     EGMELIRPLYLVREAEIKHWRDYNKLNFIQCACRFTDTCTTCSPNSNTGSKRQEIKQL
                     IANLKKINPQIESNIFHSVENVNLDTIISYKQGDNKVSFLDRYDDMGKGK"
     gene            complement(1222..2865)
                     /locus_tag="PLS64_07540"
     CDS             complement(1222..2865)
                     /locus_tag="PLS64_07540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRL43710.1"
                     /translation="MKKSEKPIGKIKVKKSIFKRLFFHILVLFILAVIVSGTLNCIEN
                     IQRTRRFTRQLTESASKVATETFERCDINALLDNPDSEEYRKAVKTLRWICQTNHLEY
                     MYIYIPNFDENKVTYVMTVADDDSENENVLNVRSAGAEVDHSLWDAEKEAWENPNLVT
                     AYEYQNDSFGSFYTAFSAIQGNDGKPVALIGADYSLTQIYTEIIFYTAMRVLALFVVL
                     AIVFLLVMRFVRKKMYNPILLIFEKIRGYFSDKADGTNTKKAFVPIKLGSDDEIQLLA
                     DYFNDMAHDVETYVEKNSALASEKAKNETELEVARRIQYGIIAREKNVVFADCFDVSA
                     RMESARQVGGDFYDCFALPDGRICAVVGDVSGKGVAAAMFMAFAKTLIHEKMTENAEP
                     DRAFEEINRELCLSNPEGMFVTAFAVIFDKNSDSFLYINAGHNKPIAVSNGKAEFLEC
                     KPCIMLGVFDDARYVVNSAEFGNGDIICLYTDGVNEATNADNEFFGNERLVSACQNAE
                     QTAKGVCDGVYDALTDFTENAEQFDDITIVAIKNSKNRD"
     gene            3246..4370
                     /locus_tag="PLS64_07545"
     CDS             3246..4370
                     /locus_tag="PLS64_07545"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HRL43711.1"
                     /translation="MEKQRLEFVTKALETNNFSALCREYEITRKTGRKWVDRFKQGLP
                     LTDMSRRPLCSPNKTPDDIEELIVSVRFDNPGWGDDKIKIFLENQGYKNIPCPRTITN
                     ILHKHQLISEEESLKRKPFKRFQKEHCNDMWQTDFKGEFLTADNRYCYPLTILDDSSR
                     YSIKIACFPNTKNVVINAFKQAFYEFGMPSSVLSDNGSQFAGFKHGFTMFEKFLMNND
                     ILPIHCRIKHPQTQGKIERFHGSMKRELLNHNLFKNLDYADKALQEWRTKYNCLRPHE
                     ALGNKCPADVYTHSERIFADKVEPFEYEPGFKVIKVNSWGYVRFQNFQLYLSETFIND
                     YVQFRPNPNNDSFLVCYRNFVIAEYDSSSGKRIHRKISRL"
BASE COUNT         1282 a          970 c          798 g         1370 t
ORIGIN      
        1 attttgcaaa ctgtcttccc gacattcatt ttcaattttc aatttttaat ttttaattac
       61 ttgcccttcc ccatatcatc atatctgtcc agaaacgaaa ctttattatc gccttgttta
      121 tatgaaataa tcgtatcgag gttgacattt tcaacgctgt ggaaaatgtt gctttcaatc
      181 tggggattga ttttcttcag atttgctata agctgtttga tttcctgtcg ttttgagcct
      241 gtgttgctgt tcggggaaca ggttgtgcag gtgtccgtaa aacggcacgc acattgaatg
      301 aaattcagct tgttgtaatc tcgccaatgc ttgatttcag cctcacgaac gaggtagaga
      361 gggcggataa gttccattcc ctcatagttt gtgctgtgga gcttcggcat catcgtctga
      421 acctgaccgc cgtagagcat acccataaga atagtttcta taacatcgtc atagtgatga
      481 ccgagtgcga ttttgttaca gccgagctgc tttgcctttt tgtagagata acctctcctc
      541 attcttgcac aaaggtagca gggatatttg tcaacattgt aaacagaatt aaaaatatct
      601 gtttcaaaaa cagttatcgg aatgttcagc ctacgggcgt tgctctcgat tacatcacga
      661 ttttcaacgc tgtagcccgg gtccattaca agaaaaacaa gctcaaacgg aaatttattg
      721 tgccttttca actcctgaaa aagctttgcc atcagcattg aatccttacc gcccgaaatg
      781 cagacggcaa ttttatcgtt cggctcaaca agcttgtact cgattatcgc cgcagtaaat
      841 ctgctccaaa tcgtcttgct gaactttttg cggatgcttt tttcaacatc ggttgcatag
      901 tcctcattct cgaattgact tctcaaccaa ctgtaataac cgccctcaag attcacggca
      961 ttgaatccgt catcacgcag attttctgca agctcgtcac ttatctgacc gcttttgcaa
     1021 cagaggacaa caagcttgtc ttttggcaaa agtgaattat cctctttgat ttttgcaagc
     1081 ggaatattct ttgcaccgtc aatgtgaccg tattcaaacg aaatttcatc tcggatatcg
     1141 acaagagtat attcctcgtc aagcttcctc acctgttcta ttgtaatctg attgttttcc
     1201 gatttaaaca ttcataaccc ttcaatctct gtttttgctg ttttttattg caacaattgt
     1261 tatatcgtca aattgctccg cattttcggt aaagtccgtc aaagcgtcat aaactccgtc
     1321 acacacacct tttgcagtct gttcggcatt ttgacaggca gacacaagtc tttcgttgcc
     1381 gaaaaattca ttatccgcat ttgttgcctc attcacaccg tcggtgtaaa ggcaaattat
     1441 atcgccgttt ccaaattcag cgctgttcac aacatatctt gcatcatcaa acacaccgag
     1501 cattatacac ggcttacatt ctaaaaattc agccttgccg ttgctcaccg caataggctt
     1561 gttatgacct gcatttatgt acaaaaagct gtcgctgttt ttgtcaaaaa tcacggcgaa
     1621 tgcagttaca aacattccct cgggatttga caaacagagt tccctgttga tttcttcaaa
     1681 tgccctgtcg ggttctgcat tttcggtcat tttttcgtgg ataagagttt ttgcaaaagc
     1741 cataaacatt gccgccgcaa cacccttgcc cgacacatcg ccgactacgg cacatattct
     1801 gccgtcaggc aatgcaaagc aatcgtaaaa atcgccgcct acctgtcttg cagactccat
     1861 tcttgccgaa acatcaaaac aatcggcaaa gacaacattc ttttctctcg caattattcc
     1921 gtactgaatc cgccttgcaa cctcaagctc ggtttcattc ttagcctttt cactcgcaag
     1981 ggcggaattt ttttcaacat aagtttctac atcgtgagcc atatcgttga aataatcagc
     2041 caaaagctga atttcatcgt cagagccgag ttttatcggc acaaacgctt tctttgtatt
     2101 tgtgccgtca gccttgtcgg aaaagtatcc tcgaattttt tcaaaaatca aaagtatcgg
     2161 attatacatc ttttttctga caaaccgcat tacaagcaag aatacaattg ccagcacaac
     2221 aaacaacgca agaactcgca ttgcagtata gaaaatgatt tccgtataaa tctgcgtcag
     2281 cgaatagtcc gcaccgatta atgcaacagg cttgccgtca ttgccttgaa tcgctgaaaa
     2341 tgccgtgtaa aaactgccga agctgtcgtt ctgatattcg taagccgtga caaggttagg
     2401 attttcccac gcctcttttt cagcgtccca caagctgtgg tcaacctctg cacctgcact
     2461 tctcacattt aaaacattct cgttttcgct gtcatcatcg gcaacagtca taacatatgt
     2521 aaccttgttt tcgtcaaaat tcggaatgta aatgtacata tattcaaggt gattcgtctg
     2581 acaaatccac ctcaaggtct taacggcttt ccgatattct tcggaatcgg gattatcaag
     2641 caatgcgttt atatcgcagc gttcaaaagt ttctgttgca acctttgacg cactttcggt
     2701 cagctgtcgt gtaaaccgcc ttgtgcgctg aatgttttca atgcaattga gcgtacccga
     2761 aacaatcacc gccaaaataa acagcacaag tatgtgaaag aaaagccttt tgaaaatcga
     2821 ctttttgact tttattttgc cgattggttt ttcggatttt ttcattaaca gtcaccgctt
     2881 tctttccaca cactttttca tcttgtattt taacacgaat gtatgataaa tgcaaaacat
     2941 aataaatgtc ataaagtaat tgtcaacccg tagcggcggt cagtgaccgc catttgtttt
     3001 gtgtgcaaac acgaaacaat tcgtagcggc gaaccgtgtt cgccgctaca aacaatccat
     3061 aattttcatc acatcaaaac attttaataa aacaaatagt ttcatcaatg cgggcgtcca
     3121 atgtgtgcag tgtagtaaca tagtatacaa cagtgcaaag acatagtata cagttttatg
     3181 ataaaataaa tggcaagaaa atagcaaatg aaggagctgt ttttatgccg tgggagtgta
     3241 aaactgtgga aaaacaaaga ttagaatttg ttacaaaagc attagaaaca aataatttta
     3301 gtgctctatg tcgggaatat gaaatcacac gcaaaaccgg tcgcaagtgg gttgatagat
     3361 ttaaacaggg actgccctta actgatatgt cacgcagacc attatgttct cctaacaaaa
     3421 cgcctgacga cattgaagaa ttaatcgttt ctgttcgttt cgataacccg ggttggggtg
     3481 atgataaaat taaaattttt cttgaaaatc aagggtataa aaacattcct tgtccaagaa
     3541 ccattactaa cattcttcat aaacaccaac tcatatctga agaggaatct ctcaaaagaa
     3601 agccttttaa acgctttcag aaagaacatt gcaatgatat gtggcaaact gatttcaagg
     3661 gagagttttt aactgccgat aatcgttatt gctatccatt gactattctc gatgacagct
     3721 cacgatactc aatcaaaata gcatgctttc ctaacactaa aaatgtcgtt attaacgctt
     3781 ttaagcaagc tttttatgag tttggtatgc catcaagcgt actttctgac aatggttctc
     3841 agtttgcagg ttttaaacat ggatttacta tgtttgaaaa attccttatg aacaatgaca
     3901 ttcttcccat acattgcaga attaaacatc cgcaaacaca aggaaaaatt gaacggtttc
     3961 atggcagtat gaaaagagaa cttttaaatc ataatctatt caagaacttg gattacgctg
     4021 acaaggctct gcaagagtgg cgaactaagt ataattgcct gcgacctcat gaagcacttg
     4081 ggaacaaatg tcctgccgat gtctatactc acagcgaaag gatttttgct gacaaggtgg
     4141 agccgtttga atatgaaccc ggctttaaag tcataaaagt aaattcgtgg ggatatgtcc
     4201 gttttcagaa ttttcaatta tatcttagtg aaactttcat caatgattat gttcaattca
     4261 gacctaatcc aaacaatgat tcatttcttg tttgctatcg caattttgtt attgctgagt
     4321 atgactcgtc atccggcaag cgtattcacc gcaagatttc aagattgtga atttgtatac
     4381 catgtccttg cacaggtggt taccatgttg cacttgacac
//
LOCUS       DAOMVE010000136         4350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5785_length_4350_cov_3.604657, whole genome shotgun sequence.
ACCESSION   DAOMVE010000136 DAOMVE010000000
VERSION     DAOMVE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5785_length_4350_cov_3.604657"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            376..1065
                     /locus_tag="PLS64_07550"
     CDS             376..1065
                     /locus_tag="PLS64_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43712.1"
                     /translation="MKISECTLSKETQDLLYRYFAAAANLYGVIEIGKLLKIYNSQNE
                     PVSEEDFLSFVENMNFKGKYFCVFNQDDIYDDGSETPALEQEILAEYLYFFEFDDDSL
                     DKYEMLKYRQIGKKFCVLDKEQFLRYEDDCYFKKTPDFLEMRSFLRNETGLSKEDADD
                     YANDIMICFATQPDNSEESVYEVIMGSGIYNRDDIAFSHRKLEYLCQKVMYTSARLHV
                     LLGHTPEEIFG"
     gene            1224..1694
                     /locus_tag="PLS64_07555"
     CDS             1224..1694
                     /locus_tag="PLS64_07555"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRL43713.1"
                     /translation="MIRIMFVCHGNICRSPMAEFIFRDMVKKGEIADRFVIKSSATST
                     EEIWNGIGNPVYPPAKRELAKHGIGCDGKRAVQLKKSDYDKYDYFICMDSNNIRNTMR
                     IFGDDKDGKVCKMMSFAGMNRDVSDPWYSGDFEQAYNDIYSGCKGLFEKFKGKA"
     gene            1839..2453
                     /locus_tag="PLS64_07560"
     CDS             1839..2453
                     /locus_tag="PLS64_07560"
                     /inference="COORDINATES: protein motif:HMM:NF037674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL43714.1"
                     /translation="MIKNTFYNLPEEKRQRITDAIFNEFAGSSGERVSINNIIKNANI
                     SRGSFYQYFDDKVDLVEVMTRSFIDFSLKKATEVISRTHGDIFVTYDLLFEILCECSK
                     DAKQSIIMKNLIKNIKANDSLVSEYFINRFKGVAELVSVTNNFDRSNLKYTSDEDVKC
                     LSQILTQVLKNAVFNVFVIGKPFDEVHREYHRKLEIIKTGAIAD"
     gene            2576..>4350
                     /locus_tag="PLS64_07565"
     CDS             2576..>4350
                     /locus_tag="PLS64_07565"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL43715.1"
                     /translation="MLSKISVKKPMTVLVAVVLVLVLGIVSFFKMTPDLLPNMDFPYA
                     VIMTTYAGQTPEAVETTVSKPLEQSMSAIDGVKEITSTSSDNYSLLMIEFEDGTNMDS
                     ATVDMRSSLDTIKGNWPDGVGTPYLIKINPNILPVAMTAVDFEGKSQTELSDYVTNEL
                     LNELEGIDGVASVSDKGIVTEQENVVLSQDKLDKLNKKISAALDNQFGDAEDKIAKAK
                     KELQDNIGKAKDGQGTVSSSIEQINSQQEAVSKQLADAQNKAESGKTQLLSAKMQLLD
                     RKASLTSTKTLLETAYQGLLELKTTYDELTSEQSQLTQKLEQLSKFNEQYQEIVRKMN
                     DALPDSEEYKALQQQIDELNKVLEPYGIKAPDIAKTMQTVQNSINKSAEAIQNVEISL
                     KKLGTSGDAINSALSEMSEKISQINSGIAQLDNAISGLDDKSVSVDSALALISQQQSS
                     ADFKMSSAMSTLTSKQSELNSALTQLSSAEKQIETSEKELADKKKEAKSKADVSNTVT
                     LDTISSILTAQNFSMPAGYVTDDDNNKFMVRVGDKVNDEKEMKSLVLFDTGIDGIGVI
                     HLEDVADVFVADNSDETFARINGNPG"
BASE COUNT         1446 a          802 c          915 g         1187 t
ORIGIN      
        1 tgtgcaagat tttcgccata gatatgattt attccactaa agtgataatc aaatatagtc
       61 tattttggtt attttatgca aaatttgtca caaaatataa taaaacaccg caaagcagag
      121 caagaaatct atggcgtatt tcaggtaaaa aaagcactat ataatccgct atatttactg
      181 tgcaacagtc ctctgcacgg caacctgaaa gcttacatat acacagtgct aaaccactct
      241 gcaactgtct tttagaagtt aatcatctgg caagtatctg atatctgaaa ttgttcacag
      301 acttgttgaa atgtgggatt aattatgata aactgacaat ataagtcaga gtacgatata
      361 atttgatgta gacacatgaa aatttcagaa tgcacacttt caaaggaaac acaagacctt
      421 ttatacagat attttgcggc tgcggcaaat ctttacggag ttatcgaaat cggcaaactt
      481 cttaaaattt acaacagcca gaacgagcct gtgagcgagg aagattttct gtcatttgtt
      541 gaaaatatga attttaaggg aaagtatttt tgtgtgttta atcaggacga tatttacgat
      601 gacggctcag aaacccctgc tcttgaacag gaaattcttg cagaatatct ttactttttt
      661 gaatttgatg acgatagtct tgataaatac gaaatgctga aatatcgtca aatcggaaag
      721 aaattttgcg ttcttgacaa agagcaattt ctcagatatg aagatgactg ctattttaaa
      781 aagactcccg attttcttga aatgcgttcg tttttaagaa atgaaacagg actttcaaaa
      841 gaggacgcag acgattatgc gaacgatatt atgatttgtt ttgcaactca acccgacaat
      901 tccgaagaat ctgtatacga agtaatcatg ggaagcggaa tctacaaccg tgatgatatc
      961 gcattcagcc accgaaagct tgaatatttg tgtcaaaaag tcatgtacac aagtgccaga
     1021 ctccatgttc tgctcggaca cactcccgaa gaaattttcg gttaacaggt aaaattgcat
     1081 attatagcga caattcaaaa tcattttgag gggaacatat agatgaatag tgaaaataca
     1141 gcaacacaca aaatagcata ctcaaatata gatattgcaa aatttatagc ggcatattac
     1201 ggcaaatcgg gaggaatttt gttatgataa ggataatgtt tgtgtgccat gggaatattt
     1261 gtcggtcgcc tatggcggag tttatattta gggacatggt taagaagggc gagattgctg
     1321 acaggtttgt gattaaatca tctgctacga gtacggagga gatatggaac gggattggga
     1381 atcctgttta tccgccggct aagcgtgagc ttgcaaaaca cggaatcggt tgtgacggca
     1441 agagggctgt tcagctcaaa aaatccgact atgacaagta cgattatttc atctgcatgg
     1501 actctaataa tatcagaaac actatgcgaa ttttcggcga tgacaaagac ggaaaggttt
     1561 gcaagatgat gagctttgca ggtatgaatc gtgatgtatc ggatccgtgg tacagcggtg
     1621 attttgagca ggcatacaac gatatttaca gcggatgcaa aggacttttt gaaaaattca
     1681 aaggcaaagc ttaattttgt tgttaatatg cacaaagatt tttgatcgta tatgtgcaaa
     1741 atctaaaaca catttaattg acacaataaa taaacgatgt taaaattgtg acaggttgtc
     1801 atatgactgc ctgtcacatt tattttggag gattttttat gataaaaaac accttttata
     1861 atcttcccga agaaaagcgt cagagaatca cggacgcaat attcaatgaa tttgccggtt
     1921 cgtcgggaga gagagtcagc ataaacaata ttataaagaa cgcaaacatt tcgagaggca
     1981 gtttttatca gtactttgac gataaggttg accttgtgga ggttatgaca cgctcattta
     2041 ttgacttttc gcttaaaaaa gccacagagg ttatctctcg cactcacggc gacatatttg
     2101 taacttatga tttgcttttt gaaattctat gcgaatgttc aaaggatgca aaacaaagta
     2161 tcattatgaa gaatctcatc aaaaatataa aggcaaatga cagtcttgtt tcggaatatt
     2221 ttatcaaccg tttcaagggc gttgcagagc ttgtcagcgt tacaaacaat tttgacagaa
     2281 gtaaccttaa atatacttca gatgaagatg tgaagtgtct ttcacagatt ctcactcagg
     2341 tgctgaagaa tgccgtgttc aatgtttttg taataggaaa accttttgac gaggttcaca
     2401 gggagtatca cagaaaactt gaaattatca aaacaggtgc aattgcagat taagtttaat
     2461 attcacgcaa ccggttgcgg ccgatttcaa cagaaaatgt aaacctaagt tggtagaaat
     2521 tacatttaat atgaaatata ctcatagata aaaagcacaa acaaaggagt aatcaatgtt
     2581 atctaaaatc agcgtaaaaa aaccgatgac ggtattggtt gcggttgtac ttgttctggt
     2641 tctcggtata gtgtcatttt tcaagatgac gcccgacctt ttaccgaata tggactttcc
     2701 ctatgccgtt atcatgacca cctacgcagg tcagacgccc gaggcggttg aaacaacggt
     2761 ttcaaagcct cttgaacagt cgatgtcggc aattgacggt gttaaggaga tcacctctac
     2821 ctcatcggac aactattcac ttttgatgat tgaatttgag gacggcacaa atatggacag
     2881 cgcaactgtt gatatgcgtt caagccttga cacaatcaag ggcaattggc ctgacggagt
     2941 cggaacaccg tacctcatca aaatcaatcc gaacattctc cccgttgcaa tgacggcggt
     3001 tgactttgaa ggcaagtcgc agacagagct ttcggattat gtcacaaacg aacttttgaa
     3061 tgaacttgaa ggcattgacg gtgttgcctc tgtttctgac aagggcattg ttacggaaca
     3121 ggaaaatgtc gtactttcac aggataagct tgacaagctt aataagaaga tttctgctgc
     3181 actcgataat caattcggtg atgcggagga caaaattgcc aaggctaaaa aggaattgca
     3241 ggataatatc ggcaaagcca aggacggtca gggtactgtt tcatcttcaa ttgagcaaat
     3301 caactcacag caggaggctg tttcaaaaca gcttgccgat gcacagaaca aggctgaaag
     3361 cggtaaaaca cagcttttgt cggcaaagat gcagttgctt gaccgaaagg caagcctcac
     3421 ttcaacaaag acattgctcg aaaccgctta tcagggtttg cttgaactga aaacgaccta
     3481 tgatgagctg acttccgagc agtcacagct tacgcaaaag cttgaacagc tttcaaaatt
     3541 caacgaacag tatcaggaaa ttgttcggaa aatgaatgat gcactgcccg acagcgagga
     3601 atacaaggca cttcaacagc agattgacga gcttaacaag gtgcttgagc cttacggaat
     3661 caaagctccc gatatcgcaa aaacaatgca gacagttcag aattccatta acaaaagtgc
     3721 agaggcaatt caaaatgttg aaatttcact taaaaagctc ggaacttcgg gagatgcaat
     3781 caattctgca ttaagtgaaa tgtcggagaa aatctcacaa atcaactcgg gtattgcaca
     3841 gcttgataac gcaatttcgg gacttgacga taaatcggtt tcggttgatt ccgctcttgc
     3901 cctcatttca caacaacagt caagtgcgga cttcaaaatg tcctctgcca tgtcaactct
     3961 cacttcaaaa cagagtgaac tcaacagcgc acttacacag ctttcaagtg cagaaaaaca
     4021 gattgaaaca tccgaaaaag aacttgcaga taaaaagaaa gaggcaaaaa gcaaggctga
     4081 tgtgagcaac acggtcacac ttgataccat ttcttcaatt ctaacggcac aaaacttttc
     4141 aatgccggca ggctatgtta ccgatgacga taacaataaa ttcatggttc gtgtcggcga
     4201 caaggtgaat gatgaaaaag agatgaagtc gcttgtgctt tttgacacag gaattgacgg
     4261 aataggagtt attcatcttg aagatgttgc agatgttttt gttgccgata attccgacga
     4321 aacctttgca agaatcaacg gcaaccccgg
//
LOCUS       DAOMVE010000137         4303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5873_length_4303_cov_3.124058, whole genome shotgun sequence.
ACCESSION   DAOMVE010000137 DAOMVE010000000
VERSION     DAOMVE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5873_length_4303_cov_3.124058"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..521
                     /locus_tag="PLS64_07570"
     CDS             162..521
                     /locus_tag="PLS64_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43716.1"
                     /translation="MSYSKNHSNFDYCCSFYVRKKGVNFLFDAICKCGYIAFENVLID
                     ETDMRLLCDTAEDALEILKKYNPKNKGLTVDDATVTIVEIIFSDKNSMSVSMTEQTLD
                     SLEKAFFALAEKYCKDV"
     gene            681..1055
                     /locus_tag="PLS64_07575"
     CDS             681..1055
                     /locus_tag="PLS64_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HRL43717.1"
                     /translation="MQTIDEALNKLKKSDFRSRFHLKQKDIDYINEKGMDKIRSHAED
                     FVRTRLAPKYIPNDGKQTPMRGHPVFIAQHACACCCRGCLNKWYRVAKDKQLNEVEQQ
                     KIVNLLMAWIEREYNDYNSNKE"
     gene            1073..2878
                     /gene="recQ"
                     /locus_tag="PLS64_07580"
     CDS             1073..2878
                     /gene="recQ"
                     /locus_tag="PLS64_07580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HRL43718.1"
                     /translation="MSDKLSVLKDYFGHDSFRDGQEQIVDALLDGRDALCIMPTGAGK
                     SMCYQIPALLFDGVTIVVSPLISLMKDQVGSLVQSGVPAAYINSSLSYPQFLRVLSNV
                     EHGKYKIIYVAPERLLTDGFLDTCKKIKISMVAVDEAHCVSQWGQDFRPSYLKIISFV
                     ESLANRPIVGAFTATATNDVKEDIKKILRLENPFEITTGFDRPNLFFGVIKSSSKDEK
                     LIDLIRERGDRSGIVYCATRKNVESVCELLCDNGFSAARYHAGLDEYERRKNQEDFVF
                     DRKNIMVATNAFGMGIDKSNVTYVIHYNMPKNIESYYQEAGRAGRDGGEADCILLYSP
                     KDVRLNRFMIENSEGNDELTIEENEQIRERDFERLKYMTFYSTTNDCLRGFILRYFGG
                     EKKAYCGKCSNCLSVHKLVDVTIDAQKIMSCIARTGQRYGKTVICDVLKGSKSEKILK
                     AELNNQSTYGIMKEVTARHIFGTIDFLAEKEYISSDNETEVLKLLPKSRDVLFGRERL
                     VMKKVENSEKVVKTHRPEVPVNSELLDALKALRKGIASKKSVPAYVIFTDATLIDMCK
                     KCPETPDEMLEVSGVGRTKLEKFGKQFLEEIAKFR"
     gene            3008..4120
                     /locus_tag="PLS64_07585"
     CDS             3008..4120
                     /locus_tag="PLS64_07585"
                     /inference="COORDINATES: protein motif:HMM:NF022635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HRL43719.1"
                     /translation="MADIFDYISWRGDLTFEQDGFNELDALVLSRLSYLPFDGAVSSC
                     LFDEITLEQAAMRVFATDDYEKNLLWKGDADLLKATAESKRFGKILVSGYVNEVESDV
                     SMQFSAITFEFLKKNYFISYRGTDFSLVGWQEDFNMFFTFPLPSQKKALDYFEKAVRM
                     LNGKFILGGHSKGGNLAVYAGAFTDENSRNHIDYIYNFDGPGFSLDKIRDSGFYEIDD
                     RIYTFVPQSSIFGMIFEHEESYTIVKSNQKGFLQHDIYSWEIEQNSLIRLKSTTNFSV
                     FFDHTLKEFVESLTIAQRREFTKEVFALLSLTETATFNEMLKNPLKNTGTILKSFAGL
                     DSKTRNMLLKTIFAFVKSAKNNFSDITGGQNKITVS"
BASE COUNT         1356 a          802 c          940 g         1205 t
ORIGIN      
        1 gcggcaattg tttgctcggc ttgcggatat aagcctgacg gcaatccgcc gaaattctgt
       61 ccgttctgcg gggctgccat gacataaccg atatgacttt acggccgtta tttcataata
      121 tttccaaacg aaaaacgcgt gtaaaaaaag aaattgtatc cgtgagctat tcaaaaaatc
      181 attcaaattt tgactactgc tgttcttttt atgtgcgaaa aaaaggtgta aatttccttt
      241 ttgacgccat ctgcaaatgc ggttatatcg cttttgaaaa tgttttgatc gacgaaacgg
      301 atatgagact gctgtgtgac accgccgaag acgcattgga aattctgaaa aaatataatc
      361 caaaaaacaa aggactgaca gtcgatgacg ccacggttac aatcgttgaa atcatatttt
      421 ccgacaaaaa cagtatgtcg gtcagtatga cggaacaaac cttggattcg ctcgaaaaag
      481 cgttctttgc ccttgctgaa aaatattgca aagatgttta atatctaaaa ataattgaaa
      541 agcaacccta tgaaaacaac cccacggtaa aataatcgtg gggttgtttt ttctgtaaca
      601 gaaaataagt gcatttttcc ttgccattac cgtagtaatt gtttataatt aaggtatgac
      661 aataaaagaa ggcatgaaca atgcaaacga tagatgaggc actaaacaaa ctgaaaaaat
      721 ctgattttcg cagtcggttt catttaaagc aaaaggacat tgactatatt aatgaaaagg
      781 gaatggataa aatccgctcc catgcagaag attttgtcag aacacggctt gcaccaaaat
      841 atattccgaa tgacggaaag caaacgccta tgaggggaca ccctgttttt atcgcacagc
      901 acgcctgtgc ctgttgttgc agagggtgtc ttaacaagtg gtacagagtt gcaaaggaca
      961 aacagcttaa cgaggtcgaa cagcagaaaa ttgtaaatct gcttatggct tggattgaaa
     1021 gggaatacaa tgattataat tccaataaag aatgatagat aagggcaaaa agatgagtga
     1081 taaattaagc gtgttaaaag attacttcgg acacgattct ttcagagacg gacaggaaca
     1141 gatagttgac gctttgcttg acggcagaga tgcactttgc attatgccca caggtgcggg
     1201 caagtctatg tgctatcaga ttcccgcatt actttttgac ggggtgacaa ttgttgtgtc
     1261 acctctgatt tcgcttatga aggatcaggt cggctcactc gttcagtcgg gagttcctgc
     1321 ggcatacata aacagctcgc tttcatatcc gcaatttctg cgagtgcttt caaatgttga
     1381 acacggaaaa tacaaaatca tctatgttgc acccgaaagg ctgttgactg acggatttct
     1441 cgacacctgt aaaaagataa aaatctcaat ggttgcagtt gacgaggctc attgcgtttc
     1501 acaatgggga caggatttca gaccgagtta cctcaaaatt atttcatttg ttgaaagcct
     1561 tgcaaatcgt ccgattgtcg gagcatttac cgcaacggct acaaatgatg taaaagaaga
     1621 tataaagaaa attctcaggc ttgaaaatcc gtttgaaatt acaacgggat ttgaccgccc
     1681 caaccttttc ttcggtgtaa taaagtcatc gtcaaaggac gaaaagctta ttgacctcat
     1741 tcgggagcgt ggcgacaggt cgggaattgt ctattgtgca acccgaaaaa atgttgaatc
     1801 tgtctgcgaa ttgctttgcg ataacggctt ttcggcggca agataccatg caggtcttga
     1861 tgaatacgaa cgcaggaaaa atcaggagga ttttgttttt gaccgaaaaa atattatggt
     1921 cgcaaccaat gctttcggta tgggaattga caagtcaaat gttacatatg taattcatta
     1981 caatatgccg aaaaacattg aaagctacta tcaagaggca ggacgggcag gtcgtgacgg
     2041 cggtgaggct gattgtattt tgctctacag tcctaaggat gttcgcctaa atcgctttat
     2101 gattgaaaat tccgagggca acgatgagct tacaattgaa gaaaacgagc aaattcgtga
     2161 gcgtgatttt gaacgcttaa aatatatgac cttttacagc acaacaaacg attgtctgcg
     2221 tgggtttatt ctgcggtatt tcggcggtga aaaaaaggct tactgcggaa aatgctccaa
     2281 ctgtctttcc gttcacaagc ttgttgatgt cacaattgac gctcaaaaaa ttatgtcgtg
     2341 tattgcaaga acaggacaaa ggtacggcaa aaccgtgatt tgtgatgtgt taaagggcag
     2401 taaaagcgag aaaattctaa aagctgagct taacaatcag tcaacctacg gaataatgaa
     2461 agaggttacg gcaaggcata ttttcggcac aattgacttt cttgccgaaa aggagtatat
     2521 ttcatccgac aatgaaaccg aggttctaaa acttttgcca aagagccgag atgtgctttt
     2581 cggacgggaa cggcttgtga tgaagaaagt tgaaaactcc gaaaaggttg tcaaaacgca
     2641 ccgccccgaa gtgcctgtta attccgaatt acttgacgca ctcaaggctt tgcgaaaagg
     2701 cattgcatcc aaaaagagcg ttcctgccta tgttattttt accgatgcaa cgctgattga
     2761 tatgtgcaaa aaatgccccg aaacacccga tgaaatgctt gaagtttcgg gtgtgggcag
     2821 aacaaagctt gaaaaattcg gaaaacaatt tcttgaagaa atcgcaaaat tcagataact
     2881 tcatatactg ttaaaggatg aacgaattcg tttgtccttt tctttttcaa tttttaaaat
     2941 tgcattgaaa taattagtac aaagtgatac aataattaca gagagaaaac agattggagg
     3001 catttgtatg gctgacatat ttgattacat aagctggcgc ggcgatttga catttgaaca
     3061 ggatggtttt aacgagcttg acgcacttgt gctttcacgg ctttcatatc ttccttttga
     3121 cggtgcggta tcgtcctgtc tgtttgatga aattaccctt gaacaggcgg caatgcgtgt
     3181 gtttgcaacc gatgattacg agaaaaatct attgtggaag ggtgacgccg accttttgaa
     3241 agcaacggca gagagcaaga gattcggcaa aattcttgtt tcggggtatg taaacgaggt
     3301 tgagtccgat gtgagtatgc agttttccgc aatcactttt gaatttctca aaaagaatta
     3361 ttttatttca tacagaggaa cggacttttc tcttgtcggc tggcaggagg acttcaatat
     3421 gttcttcaca tttccgttgc cgtcacagaa aaaggctctt gattattttg aaaaggcagt
     3481 ccgtatgctc aacggaaaat tcattctggg cggtcactca aagggcggaa atcttgcggt
     3541 ttatgccggt gcttttaccg atgaaaattc aagaaatcac attgattata tttacaattt
     3601 tgacggaccg gggttttctc ttgacaaaat cagggacagc ggattttatg aaatcgatga
     3661 cagaatctac acctttgttc cgcagtcaag cattttcggc atgatatttg agcatgagga
     3721 gagctacaca attgtaaaga gcaatcaaaa aggttttctt cagcacgata tctattcatg
     3781 ggagattgag cagaacagtc ttatcaggct gaaaagcact acaaatttca gcgttttctt
     3841 tgaccacacg ctgaaagaat ttgttgaatc gctgacaatt gctcaaagac gggagttcac
     3901 aaaagaggtt tttgcccttt tgtcgctgac cgaaaccgca acctttaatg agatgctgaa
     3961 aaatccgctt aaaaacacgg gaacaatcct aaaatccttt gcaggtcttg acagcaaaac
     4021 aagaaatatg cttttgaaga caatcttcgc attcgttaaa agtgcaaaaa acaatttttc
     4081 cgacataaca ggcggtcaga acaaaattac cgtcagttaa ttataaaaaa gctcatatat
     4141 gctccgatac tttttagcgt cggagcattt tctttgcaaa aaatcatttg tcaagacgaa
     4201 attcccgttt tgtcaattgg cacttttgga atttgtattg tcagtcttga aaatttttca
     4261 taaaatacaa aaactcccga cacggtgttt gctgtgtcgg ggt
//
LOCUS       DAOMVE010000138         4282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5927_length_4282_cov_1.854270, whole genome shotgun sequence.
ACCESSION   DAOMVE010000138 DAOMVE010000000
VERSION     DAOMVE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5927_length_4282_cov_1.854270"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>257)
                     /locus_tag="PLS64_07590"
     CDS             complement(<1..>257)
                     /locus_tag="PLS64_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL43720.1"
                     /translation="DEGDFANAVENYKSAYEIADDDNAEVCLRDYAIALVKGKRFSVA
                     QSVISMAEEKFPNSSVNKLLQAEIEYGNGNLENSLNYANEC"
     assembly_gap    258..267
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<268..>981)
                     /locus_tag="PLS64_07595"
     CDS             complement(<268..>981)
                     /locus_tag="PLS64_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRL43721.1"
                     /translation="LRQLCEVLDYLHCNNPPIIHTDIKPANIIVRDNGDICLIDFGIS
                     LSADDAVKGYSKNYSSPEQYMNVMNILNGTPEFNAPLDGRTDIYSLGAAYYHLMTGCI
                     PDIEDETLPPLEEYELPYSQPFVSIISKAMEWDIKKRYKSAAKMLSAVDNIKKQDERY
                     KRYILLQVLSSVVSGIMIVSGVLMFVFGNYEIAGTELAGQYDLFVSYCDKGDTVNAER
                     LGTDLLNNSKYRSVMTDKTT"
     assembly_gap    982..991
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<992..1314)
                     /locus_tag="PLS64_07600"
     CDS             complement(<992..1314)
                     /locus_tag="PLS64_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRL43722.1"
                     /translation="MLFGGDIVNDTYSIVEEIGSGGMGVVYLAYHLRLEKYVVMKKIK
                     NRNLDSSFLRNEVDILKKLHHPYLPQVYDIIEYECDFYTVIDYIDGKDLEHFINDGYV
                     FTESQ"
     gene            complement(1390..>1741)
                     /locus_tag="PLS64_07605"
     CDS             complement(1390..>1741)
                     /locus_tag="PLS64_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43723.1"
                     /translation="DNLDKSKLVVTLDNKKLAEGEDYTIVDDKDGSIAGMLTAEIVLK
                     AETGGIKENLKVTIGDLAGNTGEKSVDNFILSANIFQRFFANPVLVICTFAGLALVIA
                     AVIFFVAKKRKKAE"
     assembly_gap    1742..1751
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1752..>4282)
                     /locus_tag="PLS64_07610"
     CDS             complement(<1752..>4282)
                     /locus_tag="PLS64_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRL43724.1"
                     /translation="ADKTEWYSGNIEFNISAKDSSDNKHHTGIKSVTATFNGTDVSKK
                     LKGLPVFTAEKVESVNDITFNTEGLNLNEGTNTVEVTVVNNSGMSSTKEYKVYVDKIA
                     PEITSFDIQNKDKQKIVDLTPEKQHYNYYFQTDTQVTVNADDNAEDKGNYSGIKTIYF
                     RAYDVVTGEEYKSSKNVSTDNDTASATFTVKANFKGELYAYAVDNVDNNGKTQHGEKK
                     PDDLIVETQQHHNGNASVTMSRAKASYKDNSGLDLYSGNVNARVVFEDAYAGIKDAEI
                     KVSDYRNTVTNTISVSVDNNGNITSSGNGSVTVTGTKAKNTNDNLITNIVIDYVVTDN
                     SNNIKITTSMTDRAGNKTDNVKDSLSIDKTKPVIDVKYDNNEHTTYGGNDYYKADRTA
                     TVTVTERNFDESLVEATIMRNGGRYTTIGGWTHNNNTADPDKLTHVAKIVYNTDGDFT
                     FDIAVKDKAMNSADKFTQQKFTVDKTAPVIDVSFDNNSAKNGNYYKADRTATIKITEH
                     NFNSGSQYVNIPVTAEGTTAPSVVGWSGSGDDHNATVSFNKDGKYSFTVDYTDLAGNK
                     AVQKKVDSFYIDKTAPEVEITGVADHQAYNGTVAPVVTYRDDNFTDDHDFKFTRIDIN
                     SKSDDTSKFDYDTDGNGVTEFIYKYRDFAEVLENDGIYNFTVELSDKAGNSTSKSVTF
                     SVNRFGSTFKASDESEKLINNGYTNAEQDIVVEEINVTPLTKHSVTLAKSGGNSTELV
                     ENTDYTFTSSNNGNEWCKSVYTVNKKNFSDEAAYTVTIMSVDKAKNTNNNRMADSSLS
                     TEQKNKRECAISFVVDKTSPLVSITGIKDNELYKEASKKVKIVCE"
BASE COUNT         1182 a          917 c          735 g         1418 t
ORIGIN      
        1 acattcattt gcataattta aagaattttc caaattgccg tttccatatt caatttccgc
       61 ctgaagtaat ttattgacag atgaattcgg aaatttttcc tctgccatag aaataacgct
      121 ctgagccact gaaaatcttt taccttttac aagggcaatt gcgtaatctc tcaagcaaac
      181 ttcggcattg tcatcgtcag cgatttcata tgccgactta taattttcaa cggcattggc
      241 aaaatcgccc tcgtcaannn nnnnnnngtt gttttatcgg tcataacact gcgatatttt
      301 gaattattca gcaaatccgt tccgagccgt tcagcattaa ccgtgtcgcc tttatcacaa
      361 tatgaaacaa acaaatcata ctgccctgca agctcggtac ctgcaatttc ataattaccg
      421 aacacaaaca taagcacgcc ggaaactatc ataattcccg aaacaaccga agacaaaacc
      481 tgcaataata tgtatctctt ataacgctca tcctgttttt tgatattgtc aacagccgaa
      541 agcatttttg ccgcagattt atagcgtttc tttatgtccc actccatagc cttgcttatt
      601 atcgaaacaa acggctgaga atacggaagt tcgtattctt caagaggcgg aagtgtttcg
      661 tcctcaatat cgggaataca ccctgtcata agatgataat aagcagcacc aaggctgtaa
      721 atgtctgtcc gtccgtcaag cggagcgttg aattccggag ttccgttcaa tatattcatc
      781 acattcatat actgctcggg tgatgaataa tttttgctgt aaccctttac agcgtcatcg
      841 gcactcaatg aaatgccaaa gtcaattaag caaatgtcgc cgttatccct cacaattata
      901 tttgcgggtt taatgtccgt atggataatc ggaggattat tgcaatgcaa atagtcaagc
      961 acctcacaaa gctgtcggag cnnnnnnnnn naactgactt tccgtaaaca cataaccgtc
     1021 attgataaaa tgttcaagat ccttgccgtc aatataatca ataacagtat aaaaatcaca
     1081 ttcgtattcg atgatgtcat atacctgcgg aagatacgga tgatgaagtt ttttgagaat
     1141 atccacttca tttcgcagaa aggaagaatc aagatttctg ttcttgattt ttttcataac
     1201 aacatatttt tccagtctga ggtgataggc aagatacacc actcccatac cgccgctgcc
     1261 gatttcttca acaatactgt aagtatcatt tacgatatca ccgccgaaaa gcatatctta
     1321 cccacctttc tttttcgggc atatcccgaa aagaggatac gcccgaatca ttaaaaatat
     1381 tgtctgactt tattcagcct tttttctttt ctttgcaaca aagaagatta ctgccgcaat
     1441 aacaagtgca agacctgcaa aagtacaaat taccagtacg ggatttgcaa agaatctctg
     1501 gaatatatta gcggaaagaa tgaagttatc aacactcttt tcacctgtat ttcctgcaag
     1561 gtcaccgatt gtaaccttaa gattttcttt aataccgcct gtttctgctt taagaacaat
     1621 ttctgcggta agcatacctg caatagaacc gtccttatca tcaacaatcg tgtaatcctc
     1681 accctcggca agctttttgt tatcaagagt tacaaccagc ttgctcttat caagattatc
     1741 gnnnnnnnnn ncctcacaaa caattttaac ctttttgctt gcttccttgt aaagctcatt
     1801 atccttgata cccgtaatac ttacgagagg gcttgtctta tcaacaacaa atgaaattgc
     1861 acattcacgc ttatttttct gttctgtgct gagagaagaa tctgccatac ggttgttatt
     1921 tgtgttctta gccttatcaa ccgacataat tgtaactgta tatgcagcct cgtcagagaa
     1981 gttcttcttg tttacggtat aaacactctt gcaccactca ttgccgttgt tgcttgaagt
     2041 aaatgtgtaa tcggtatttt caacaagttc tgtactattt ccgccgctct tggcaagtgt
     2101 tacgctgtgc tttgtaagcg gagtaacatt gatttcttca accacgatat cctgctctgc
     2161 atttgtgtaa ccattgttaa tgagcttttc cgactcatcg gatgctttaa atgttgagcc
     2221 gaatctgttt acagagaatg taacgctctt tgatgtgctg ttgcctgcct tatcgcttag
     2281 ctcaacggtg aagttataaa taccgtcatt ttcaagaacc tcggcaaaat ctctgtactt
     2341 atagataaat tctgtcacgc cgttgccgtc ggtatcgtaa tcgaacttag aagtatcatc
     2401 actcttgctg ttaatatcga ttcttgtaaa tttaaaatcg tgatcatcgg taaagttatc
     2461 gtccctgtat gtaacaacag gagcaaccgt gccgttataa gcctgatgat ctgcaacacc
     2521 tgtgatttca acttctggag cggttttgtc gatatagaac gaatcaacct ttttctgaac
     2581 agccttgtta cctgcaaggt cggtatagtc aacagtaaat gaatacttgc cgtccttgtt
     2641 gaacgaaact gttgcattgt ggtcatcgcc tgaacctgac caacctacaa ctgacggagc
     2701 agttgtaccc tctgcagtta cgggaatgtt tacatactga cttccgctgt tgaagttatg
     2761 ttccgtaatc ttgatagtag cagtacggtc agccttgtag tagttgccgt tctttgcaga
     2821 gttattgtca aatgaaacgt caattacagg agcagtcttg tcaaccgtga acttctgctg
     2881 agtaaacttg tcggcagaat tcatagcctt gtccttaacg gcaatgtcaa atgtaaagtc
     2941 gccgtctgtg ttgtaaacaa tcttagcaac atgagttaac ttgtcgggat ctgcggtatt
     3001 gttgttgtga gtccagccgc cgattgttgt gtatctgccg ccgtttctca taattgtcgc
     3061 ctcaacaagc gactcgtcaa agttacgctc tgtaacagtt acggtagccg tcctgtcagc
     3121 cttgtaataa tcatttccgc cgtatgtagt atgctcgttg ttgtcatact taacatcaat
     3181 aacaggctta gtcttgtcga tgctcaagct gtccttaaca ttgtctgtct tattacctgc
     3241 tctgtcagtc attgatgttg taattttaat attattactg ttatccgtaa caacataatc
     3301 aataacaata tttgtaataa ggttatcatt tgtattctta gccttagtac ctgtcacagt
     3361 tacagaaccg ttccctgagg aagtaatatt accgttattg tcaacagaaa cactaatcgt
     3421 gttagtaacg gtatttctat aatcagaaac cttaatttca gcgtctttaa tacctgcgta
     3481 agcgtcttca aatacaaccc ttgcgtttac atttccgctg taaaggtcaa gtcccgaatt
     3541 gtccttgtag cttgcctttg ctcttgacat agtaacactt gcattaccgt tgtggtgctg
     3601 ctgtgtttca acaattaagt catcaggctt tttctcgcca tgctgagttt ttccattgtt
     3661 atctacatta tcaacagcat aagcataaag ctcaccttta aagtttgctt taacagtaaa
     3721 tgttgctgat gcggtatcat tatcagtgga aacattctta cttgatttgt actcttcgcc
     3781 tgtcactaca tcatatgctc tgaaataaat agtcttaatt cccgagtaat tacctttatc
     3841 ttcagcatta tcgtcagcat ttacagttac ctgagtatcc gtctggaaat aatagttata
     3901 atgctgcttt tcaggtgtta aatctacgat tttttgttta tccttatttt ggatatcaaa
     3961 gcttgttatt tcaggtgcaa ttttatcaac ataaacttta tattccttag ttgaagacat
     4021 accgctgtta tttacaactg ttacttcaac tgtatttgta ccctcgttta aatttaatcc
     4081 ctctgtatta aaggttatgt cattaacact ttcaaccttt tcagcggtaa aaacaggaag
     4141 accttttaat tttttagaaa catcagttcc attgaatgta gcagttacac ttttgatacc
     4201 tgtatgatgt ttgttatcac tgctatcctt tgcagatata ttaaactcaa tatttcctga
     4261 ataccattct gttttgtctg ct
//
LOCUS       DAOMVE010000139         4263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_5963_length_4263_cov_3.207224, whole genome shotgun sequence.
ACCESSION   DAOMVE010000139 DAOMVE010000000
VERSION     DAOMVE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4263
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5963_length_4263_cov_3.207224"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /gene="rplF"
                     /locus_tag="PLS64_07615"
     CDS             complement(<1..293)
                     /gene="rplF"
                     /locus_tag="PLS64_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRL43725.1"
                     /translation="MSRIGRKPINIPAGVTASVDNGVITVKGPKGTLDFKFNPAMTVE
                     IKGDVIEVTRPNDAKENRSLHGLTRTLIHNMVIGVTEGYSKTLEVNGVGYRVQ"
     gene            complement(309..707)
                     /gene="rpsH"
                     /locus_tag="PLS64_07620"
     CDS             complement(309..707)
                     /gene="rpsH"
                     /locus_tag="PLS64_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRL43726.1"
                     /translation="MQITDTIADLLTRIRNANSAKHDSVEIPASNLKKSICQILVDEG
                     YVKNFTVIEDGKQGVIKVTLKYTDGKTPVITGLRRVSKPGLRIYSNAEDMPKVMKGLG
                     IAIISTSKGVMTDREARKQHIGGEVLAYIW"
     gene            complement(727..912)
                     /locus_tag="PLS64_07625"
     CDS             complement(727..912)
                     /locus_tag="PLS64_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRL43727.1"
                     /translation="MAKTSMKVKQQRKQKFSTREYTRCNICGRPHAYLRKYGICRICF
                     RELAYKGEIPGVKKASW"
     gene            complement(925..1464)
                     /gene="rplE"
                     /locus_tag="PLS64_07630"
     CDS             complement(925..1464)
                     /gene="rplE"
                     /locus_tag="PLS64_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRL43728.1"
                     /translation="MARLKEYYSKEVAPALMSKFSYKSTMQIPKLDKIVINVGAGEAK
                     DNSKVIDAICTDLSAITGQKPLICKARKSVANFKLREGMAIGCKVTLRGERMYEFLDR
                     FFNVALPRVRDFRGINPDSFDGRGNYNMGLKEQLIFPEIDYDKIDKVRGMDICFVTTA
                     TTDEEARELLKLMGAPFSK"
     gene            complement(1479..1796)
                     /gene="rplX"
                     /locus_tag="PLS64_07635"
     CDS             complement(1479..1796)
                     /gene="rplX"
                     /locus_tag="PLS64_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRL43729.1"
                     /translation="MSKVHVKTGDTVIVLSGKDKGKTGQVMAVSPKEGKVIVEKVNMV
                     SKHVKPKKMGEQGGIIKAEGAMYASKVQIVCPRCKKPTRVAHKFYEDGSKGRVCAKCG
                     EAL"
     gene            complement(1811..2179)
                     /gene="rplN"
                     /locus_tag="PLS64_07640"
     CDS             complement(1811..2179)
                     /gene="rplN"
                     /locus_tag="PLS64_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRL43730.1"
                     /translation="MIQQQTILKVADNTGAKELMCIRVLGGTRRRYANIGDVIVASVK
                     KAAPGGVVKKGDVVKAVVVRSAKGVRREDGTYIRFDENAAVIIKEDKNPKGTRIFGPV
                     ARELRDKDYMKILSLAPEVL"
     gene            complement(2219..2476)
                     /gene="rpsQ"
                     /locus_tag="PLS64_07645"
     CDS             complement(2219..2476)
                     /gene="rpsQ"
                     /locus_tag="PLS64_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRL43731.1"
                     /translation="MSERNMRKTVVGKVVSDKMDKTIVVAVEDSVKHKLYNKIVKRTV
                     RFKAHDENNECGIGDRVKIMETRPLSKDKRWRLVEIIEKAK"
     gene            complement(2494..2697)
                     /gene="rpmC"
                     /locus_tag="PLS64_07650"
     CDS             complement(2494..2697)
                     /gene="rpmC"
                     /locus_tag="PLS64_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRL43732.1"
                     /translation="MKASELRDMSVEELQTKLTELKEELFNLRFQLAVNQLENSSRIG
                     AVKKDIARISTVLRQMELNGAEA"
     gene            complement(2697..3116)
                     /gene="rplP"
                     /locus_tag="PLS64_07655"
     CDS             complement(2697..3116)
                     /gene="rplP"
                     /locus_tag="PLS64_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRL43733.1"
                     /translation="MLLPKRVKYRRVHRGRMTGKALRGNKVTYGDFGLQATEPAWITS
                     NQIEAARVAMTRYCKRFGKVWIKIFPDKPVTAKPAETRMGSGKGSPEYWVAVVKPGRV
                     MFELGGVDEATAREALRLAANKLPIKCKFVKKEEGEQ"
     gene            complement(3116..3787)
                     /gene="rpsC"
                     /locus_tag="PLS64_07660"
     CDS             complement(3116..3787)
                     /gene="rpsC"
                     /locus_tag="PLS64_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRL43734.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFADKKNFGATLVEDYNLRKTLK
                     AQLYNFGIAKIEIERDGKKVKISIHCAKPGLVIGKGGAEIEKLKAQCEAMLKKPVAIN
                     IVEVKAPELDAQLVAENVAQQLEKRISFRRAMKQSIGNAMRRGAKGIKIMCSGRLGGA
                     EIARSEQYHEGTIPLQTLRADIDYGFAEANTTYGKIGVKVWLYKGEVLNEVKSRKPRR
                     EGGRK"
     gene            complement(3802..4140)
                     /gene="rplV"
                     /locus_tag="PLS64_07665"
     CDS             complement(3802..4140)
                     /gene="rplV"
                     /locus_tag="PLS64_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRL43735.1"
                     /translation="MEAKAIAKFVRISPRKVGVVLDLIRGKDAKYAEAVLKHTPKAAS
                     EPLLKLLNSAMANAENNHDMDVSRLYVAECNATAGPILKRIRPRAQGRAFRINKRTSH
                     ISLVLKEKED"
     gene            complement(4161..>4263)
                     /locus_tag="PLS64_07670"
     CDS             complement(4161..>4263)
                     /locus_tag="PLS64_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HRL43736.1"
                     /translation="VPVYVTEDMVGHKLGEFAPTRTFKGHAGSKTSK"
BASE COUNT         1053 a         1052 c          827 g         1331 t
ORIGIN      
        1 ttctgaacac ggtaaccaac gccgttaacc tcaagagtct ttgaataacc ctctgtaaca
       61 ccgataacca tattatggat aagtgttctt gtaaggccgt gaagtgaacg gttttccttt
      121 gcgtcgttag gacgtgtaac ctcgattaca tcgcccttga tttcaacagt catagcagga
      181 ttaaacttga aatcaagtgt acccttagga cctttaactg tgataacgcc gttgtcaact
      241 gaagctgtaa cgccggcggg aatatttata ggttttcttc caattcgaga catttatcgc
      301 acctcctctt accaaatgta agcgagaacc tcgccgccga tgtgctgctt tcttgcctca
      361 cgatctgtca taacaccctt tgaggttgaa atgatagcga taccaagacc cttcattacc
      421 tttggcatat cttctgcatt actgtaaatt ctgagaccgg gctttgatac acggcggaga
      481 cctgtgataa caggagtctt gccgtctgta tacttgagtg ttaccttaat aacgccctgc
      541 ttgccgtctt caataactgt gaaattcttt acataacctt cgtcaacaag aatctgacaa
      601 attgacttct tgaggtttga agcgggaatt tcaacagaat catgctttgc tgaatttgcg
      661 ttacggattc ttgtaagtaa atcagcaatt gtatctgtaa tctgcattat gctttacctc
      721 ccttgcttac cagcttgctt tctttacacc cggaatttcg cccttgtaag cgagttcacg
      781 gaagcaaata cgacaaatgc catacttacg gaggtaggcg tgtggtctac cacagatgtt
      841 acatcttgtg tactctcttg tagagaattt ctgctttctc tgctgcttta ctttcattga
      901 agttttagcc acaacaatcc ctccttactt tgaaaacggt gcgcccataa gctttaagag
      961 ctcgcgagct tcttcgtctg ttgttgcagt ggttacgaaa caaatgtcca taccacgaac
     1021 cttgtcgatc ttgtcatagt caatctcggg gaagatgagc tgttctttga ggcccatgtt
     1081 gtagttacca cggccgtcaa atgaatcagg gttgatacct ctgaagtcac gaacacgggg
     1141 aagtgctaca ttgaagaatc tgtcgaggaa ttcatacatt ctctcgcctc ttaatgtaac
     1201 cttacagccg attgccatac cttctctgag tttgaagtta gcaactgact ttcttgcctt
     1261 acagatgaga ggcttctgac ctgtaatagc agaaaggtct gtgcagatag catcaattac
     1321 ttttgagtta tcttttgctt cgcctgcgcc tacattgata acgattttat caagcttagg
     1381 aatctgcatt gtgctcttgt aggagaactt tgacataaga gcaggtgcaa cttcttttga
     1441 ataatattct ttaagtcttg ccatatctta tttcctcctt aaagtgcttc gccgcattta
     1501 gcgcaaactc tgcccttaga gccgtcctca tagaacttat gagcaactct tgtaggcttt
     1561 ttgcatcttg ggcaaacgat ctgaacctta gatgcataca tagcaccctc agccttaatg
     1621 ataccgccct gctcgcccat cttcttgggc ttaacatgct ttgaaaccat gtttaccttt
     1681 tctacgataa ctttgccttc tttagggctt acagccataa cctgacctgt cttgcccttg
     1741 tccttaccgc ttaatacgat aacggtgtcg cctgttttaa catgaacttt actcattgtg
     1801 acacctccaa ttaaagtact tcgggagcga gagaaagaat cttcatataa tccttatcac
     1861 gaagctctct tgctaccggt ccgaagatac gagtaccctt cgggttctta tcttccttaa
     1921 taataacagc tgcattttca tcgaaacgga tataagtacc gtcttctctt ctgacgccct
     1981 ttgcagaacg aacaactaca gcctttacaa cgtcgccttt tttaacaaca ccgccgggtg
     2041 ctgccttttt aaccgaggct acgattacat cgccaatgtt ggcatatctc cttctggtac
     2101 cgccgagtac acgaatgcac ataagttcct ttgcgccggt gttgtcggca accttgagga
     2161 tagtttgctg ctgaatcaca gtgtaattcc tcctttactc aaagccgcta aaataaactt
     2221 atttagcttt ctcaataatc tcgacgagtc tccatctttt gtctttagaa agaggacggg
     2281 tctccataat ctttacacgg tcgccaatgc cacactcgtt gttttcgtcg tgtgccttga
     2341 aacgaactgt acgcttaaca attttgttgt aaagcttgtg ctttacgctg tcttcaactg
     2401 caacgacaat tgtcttgtcc atcttgtcgc ttacaacttt tccgacaaca gtttttctca
     2461 tatttctttc actcactgtt aagtcctcct ctcttaagct tcagcgccgt taagttccat
     2521 ctgacgaaga actgttgaaa ttcttgcgat gtccttttta acagcaccga ttctggatga
     2581 gttttcgagc tgattaacag caagctggaa acgaagattg aataattctt ctttgagttc
     2641 agtgagcttt gtctgaagct cttctactga catatctctg agttctgatg ctttcattac
     2701 tgctcaccct cctctttctt aacaaacttg cacttgatcg gaagcttatt tgcagcaagg
     2761 cgaagagcct ctcttgcagt agcttcatct acgccgccga gttcaaacat aactctgccg
     2821 ggctttacta cggctaccca gtactcgggt gaacctttac ctgaacccat tcgtgtttca
     2881 gcaggctttg ctgtaacagg cttatcaggg aaaattttga tccatacttt accgaatctt
     2941 ttgcagtaac gagtcatagc gacacgggca gcctcaatct ggtttgaggt gatccatgca
     3001 ggctctgtag cctgaagacc gaaatcgccg taagtaacct tgttaccacg aagagcctta
     3061 cctgtcatac gacctctgtg aactcttctg tattttactc tcttaggtaa taacattact
     3121 ttctgcctcc ttctctgcgt ggctttcttg acttaacttc gtttaaaacc tcgcccttgt
     3181 aaagccatac cttaacgcca atcttaccgt aagtagtgtt agcctcggca aagccgtagt
     3241 cgatgtcagc acgaagtgtc tgaagcggaa tagtaccttc gtggtactgc tctgaacgag
     3301 cgatttcagc gccgccaaga cgacctgaac acataatttt aataccctta gcgccacgac
     3361 gcattgcgtt accgattgac tgcttcatag cacgacggaa tgaaattctc ttttcaagct
     3421 gctgagcaac attttcagca acaagctgag cgtcaagctc aggagcttta acttctacta
     3481 tattgatagc aactggcttc ttaagcatag cttcgcactg agccttgagc ttctcaatct
     3541 ctgcgccgcc cttaccgata acaaggcccg gcttagcaca gtgaatgcta attttaacct
     3601 ttttgccgtc acgctcaatt tcaatttttg cgataccgaa gttgtagagc tgagccttga
     3661 gtgttttacg gaggttatag tcctcaacga gtgttgcacc aaagttcttc ttgtctgcaa
     3721 accaacggga atcccaatct ttaattacac cgacacgcaa accgtgcgga ttaactttct
     3781 gacccatatt taaacctccc cttagtcttc tttttccttg aggacaaggc tgatgtgaga
     3841 tgttctcttg ttaattctga acgctctgcc ctgtgctctc ggacgaattc ttttaaggat
     3901 ggggcctgct gttgcgttgc attcagcaac atacagtctt gatacatcca tatcatgatt
     3961 attctcagcg ttagccatag ctgaattgag caactttaaa agtggctcgc tagcggcctt
     4021 aggagtgtgc ttgagaacag cttccgcata tttagcgtct ttacctctga taaggtcaag
     4081 aacaacgcct accttgcggg gtgagatacg aacaaatttt gcaattgctt tagcttccat
     4141 ctgcaatact ctccttccgc ttacttagat gtcttagatc ctgcgtgtcc cttaaatgtt
     4201 ctggtaggag caaactcacc gagcttgtga ccaaccatat cttctgtaac atatacagga
     4261 aca
//
LOCUS       DAOMVE010000140         4237 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6008_length_4237_cov_3.441416, whole genome shotgun sequence.
ACCESSION   DAOMVE010000140 DAOMVE010000000
VERSION     DAOMVE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6008_length_4237_cov_3.441416"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLS64_07675"
     CDS             <1..856
                     /locus_tag="PLS64_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HRL43737.1"
                     /translation="TNPELRCKMMNNRFAGDTLKYLKRFADAGITLNCQIVSCPGIND
                     GDELVRTLTDLENLGVNMTAIVPVGLTRYRENLYPLVPYNKETAGQTIDIIEKMGDEC
                     VKKHGRRIFFPGDEFYLLAEREIPSPEFYEDFSALEDGIGMIAYLTDDVGWKLEELDA
                     DESLCHKVTIACGEGVFPYMKRIMSMINEKFPNITINTRAIKNNFFGGGVNVSGLVTG
                     GDLIDQLRDDDLGDRLIITSSMLRFENDLFLDDVSTDDVERELGVTLVPVNNNGNDLV
                     EAVIAGKD"
     gene            861..2183
                     /gene="der"
                     /locus_tag="PLS64_07680"
     CDS             861..2183
                     /gene="der"
                     /locus_tag="PLS64_07680"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRL43738.1"
                     /translation="MNKPVIAVVGRPNVGKSTLFNKLIGQRLSIVDDTPGVTRDRIYG
                     EVEWCGKTAFIVDTGGIEPKSDDIILVQMRRQAQLAIDTANVIILVTDCKSGMVATDM
                     DVAQMLQKSGKPVVLCVNKCDTLGEPSPEFYEFYNLGLGDPIEVSAVHGYGTGDLLDK
                     AFSSFDYDENAEDNDTIINVAVIGKPNAGKSSLVNKITNEERCIVSDIAGTTRDTIDT
                     LVENKYGKFNFTDTAGLRRQSKIYDNIEKYSIIRAKMAIERSDVCVIMIDATEGVTEQ
                     DTKVAGLAHEAGRACIIAVNKWDAVEKNDKTMQEFRKKLDADFAFMSYAPKVFISAKT
                     GMRIDRLFEYIISCNDSANRRIRTGMLNELLAQATTRVQPPSDKGKRLKIFYMTQASV
                     KPPTFVFFCNNAQLFHFSYQRYLENRIREAFALEGTPIKIVVRERGEK"
     gene            2183..2908
                     /gene="plsY"
                     /locus_tag="PLS64_07685"
     CDS             2183..2908
                     /gene="plsY"
                     /locus_tag="PLS64_07685"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRL43739.1"
                     /translation="MEHISAILANCWWMILLSALIAYLLGSINTAVLVTGIVTKGKKD
                     IRQMGSGNAGFTNVLRSVGKVPAIITIVCDALKCIIAVLIGGFIFSFASVAFQGESPI
                     FINELINCGKYVAGIFCILGHSYPVYFHFKGGKGVVTAAALMLTEDWRVFIAIIVTFL
                     IIFLCSKIISLASVLCAILYAPYTFAMTFIFDFIIYKDYSLTYVIFSTAAALLIGVFV
                     LVKHKENIKRLLKGEEKKITAKK"
     gene            3022..3363
                     /locus_tag="PLS64_07690"
     CDS             3022..3363
                     /locus_tag="PLS64_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRL43740.1"
                     /translation="MSDCIFCKIVDGSIPSKKVYEDEKILAFYDLEPQAPVHVLVIPK
                     EHICCADAVNEENVGNIAHIFTKIPEITKSLGLTNGYRIVNNCGEDGCQSVKHMHFHI
                     LGGKKLNGTMA"
     gene            3379..3915
                     /locus_tag="PLS64_07695"
     CDS             3379..3915
                     /locus_tag="PLS64_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRL43741.1"
                     /translation="MDTYKINIEGLDRELRKFPVNDKLDIAAFIMFGDIEMTEVAARE
                     LLKRVPEHDVVVTAEAKGIPLCYEMARQGCRNYVVARKSVKVYMGNPIGVTVNSITTK
                     NEQKLYLGEDDVNLLNGKRVLLVDDVISTGESLHALETLVEKTGANIVGRASVLAEGD
                     AKDRDDIIFLSELPLFFK"
BASE COUNT         1297 a          808 c          944 g         1188 t
ORIGIN      
        1 aacaaacccc gagcttcgtt gtaaaatgat gaataaccgc tttgcaggcg atacattgaa
       61 atatctcaaa cgctttgcag acgcaggaat aacgcttaac tgtcagattg tttcctgtcc
      121 cggaattaat gacggtgacg agcttgtcag aacattgacc gatcttgaaa acctcggtgt
      181 taatatgact gcgattgttc ctgtcggact tacacgctac cgtgaaaacc tttatccgct
      241 tgttccttat aataaggaaa cggcagggca gacaattgat ataattgaaa aaatgggtga
      301 tgagtgcgtc aaaaagcacg gcagacgaat tttctttccg ggtgatgaat tttacctcct
      361 tgccgaaaga gaaatcccgt cacctgaatt ttatgaggac ttttccgctc ttgaggacgg
      421 aatcggtatg attgcgtatc tgaccgatga tgtcggctgg aagcttgaag agcttgatgc
      481 agacgaaagc ctttgccata aggtaacaat tgcctgcggc gagggagtgt ttccgtacat
      541 gaagcggatt atgtcgatga ttaacgaaaa attcccgaat ataactataa atacaagggc
      601 gattaagaac aactttttcg gcggcggagt aaatgtgtcg ggacttgtca caggcggtga
      661 tcttattgat cagctccgtg acgatgacct tggtgacagg cttattatca cgtcatcaat
      721 gctccgtttt gaaaacgatt tgtttcttga tgatgtttca acagatgatg ttgaacggga
      781 actcggtgta actcttgttc ccgtcaataa caacggaaac gaccttgtag aggctgtaat
      841 tgcaggaaag gactgataag atgaacaaac ccgtaattgc cgttgtcggc agaccaaatg
      901 tgggaaagtc aacgcttttc aataaactca tcggtcagag attgtcaatt gttgacgata
      961 ctcccggtgt tacccgtgac agaatatacg gtgaggtgga atggtgcgga aaaactgcct
     1021 ttattgttga cacaggcggt attgaaccga aatcagatga tattattctt gtgcagatgc
     1081 gccgtcaggc tcagcttgca atcgacacgg ctaatgttat tatccttgta acagactgca
     1141 agtcgggcat ggttgcaacg gatatggatg ttgcgcagat gctccagaag tcgggcaagc
     1201 ctgttgtgct ttgtgtaaac aagtgcgaca cactcggcga accgtcacct gaattctatg
     1261 agttttataa tctcggttta ggcgatccta tcgaggtttc agccgttcac ggttacggta
     1321 cgggcgacct tttggacaag gctttttcaa gctttgatta tgatgaaaat gcggaagata
     1381 acgacacaat catcaatgtt gcggtaatcg gcaagccgaa cgcaggcaag tcatctcttg
     1441 taaacaaaat tacaaacgag gaacgctgta ttgtgtctga tatagcaggc acaacccgtg
     1501 acacaatcga cacgcttgtt gaaaataagt acggaaagtt taactttacc gataccgcag
     1561 gacttcgccg tcagagcaaa atatatgata atatcgaaaa atacagtatt atccgtgcaa
     1621 aaatggctat tgaacgcagt gatgtgtgcg ttattatgat tgatgcgaca gagggcgtta
     1681 cggaacagga taccaaggtt gcaggtcttg cgcatgaggc aggcagagcg tgtataattg
     1741 ccgtaaacaa gtgggacgct gtcgaaaaaa acgataagac aatgcaggag ttccgcaaaa
     1801 agcttgacgc agattttgcg tttatgtcat atgcgccaaa ggtatttatt tccgcaaaaa
     1861 caggtatgcg tatcgacagg ctgtttgaat atatcataag ctgtaatgac agcgcaaacc
     1921 gcagaatcag aacaggtatg ctcaacgaac ttcttgcaca ggctacaaca agagttcaac
     1981 cgccgtcgga caagggtaag cgacttaaaa ttttctatat gactcaggct tccgtaaaac
     2041 cgccgacatt cgttttcttc tgcaacaatg cacagctttt ccacttctca tatcagcgtt
     2101 atcttgaaaa cagaatccgt gaggcttttg ctcttgaggg tacgccgatc aaaattgtgg
     2161 taagagagag aggagaaaag taatggagca tatttcggca attttagcaa actgctggtg
     2221 gatgattctg ctttcggcat tgattgcgta tcttctcggc agtatcaaca cggctgttct
     2281 tgtcacaggc attgtaacaa agggcaaaaa ggatatccgt caaatgggca gcggcaatgc
     2341 aggctttaca aatgtactgc gcagtgtcgg taaagttccg gcaatcatca ccattgtgtg
     2401 tgacgcactg aagtgtatta tcgccgtgct tatcggaggt ttcatcttct cgtttgcatc
     2461 ggttgcattt cagggtgaaa gccctatttt tataaacgag cttatcaact gcggaaaata
     2521 tgttgcaggt attttctgta ttctcggaca ttcatacccc gtgtattttc actttaaggg
     2581 cggcaagggt gttgtaaccg ccgccgctct tatgctgaca gaggattggc gtgtatttat
     2641 cgcaattatt gtaacctttc ttattatctt cctctgctca aaaattatat cacttgcaag
     2701 tgttctctgt gcgattctct atgcgcctta cacctttgca atgacattta tctttgactt
     2761 cataatttac aaggattatt ctcttacata tgtaatcttc tcaactgcgg cggctctttt
     2821 aatcggcgtt ttcgttcttg taaagcacaa agaaaacatc aaaagacttc ttaaaggtga
     2881 ggaaaagaaa attacagcca aaaaatgacg gcagtattga ccgaaaagtg atttggttgt
     2941 acaataactt tggtatgata tttgttatat tttaaatatt tctgatacgg aaaataaatt
     3001 atattgagag gttgaattaa aatgtcagat tgtattttct gcaaaatcgt tgacggctca
     3061 attccgtcaa aaaaagttta tgaagacgaa aaaattctcg ctttctatga tcttgaacca
     3121 caggctcccg ttcatgtgct tgtaattcca aaggaacaca tctgctgtgc agacgcagtt
     3181 aatgaagaaa atgtcggcaa tattgctcac attttcacta aaattcccga aattacaaaa
     3241 tcactcggac ttacaaacgg ctaccgtatt gtaaataact gcggtgagga cggctgtcag
     3301 agcgtaaagc atatgcattt ccacatcctc ggcggcaaaa agctcaacgg cacaatggca
     3361 taagaaggga ctgcaataat ggatacatat aagattaata ttgaaggact tgacagagaa
     3421 ctcagaaaat tccctgtaaa cgataagctt gatattgccg catttattat gtttggtgac
     3481 attgagatga cagaggttgc cgcaagagaa ctcctcaagc gtgttcccga acacgatgtt
     3541 gttgtgactg ccgaagcaaa gggtattccg ctttgctatg aaatggctcg tcagggctgc
     3601 agaaactatg ttgtcgcaag aaaaagcgtt aaggtttata tgggcaatcc tatcggagtg
     3661 accgttaatt caatcacaac aaaaaacgaa cagaaactct acctcggcga agatgatgta
     3721 aatcttctca acggcaagag agttcttctt gttgatgatg taatttcaac aggcgaaagt
     3781 cttcacgctc ttgaaacgct cgttgaaaaa acaggcgcaa atatcgttgg cagggcgtca
     3841 gtccttgccg aaggtgacgc taaggacaga gatgacatta tattcctcag cgaacttccg
     3901 cttttcttta agtaatactg agtaattata aaatcaaatt taaacagctg ttgaagtgtg
     3961 cagattatac cgtccgcata ttttgacggc tgttttcctt ttttatacca tagatataaa
     4021 gtgtaaaaat taaagtgtag aaattgttca acttataaat gattgaatag aaacagtggt
     4081 gaatagataa tttaatgaga agagatagca gcaaaattaa caaaaataaa aatactttta
     4141 aaaaataata tttatattga caaatgaaat tctatatgat attataaaaa tacaaaaaat
     4201 gatactagaa taattgtaga tgtaaatcca agtaata
//
LOCUS       DAOMVE010000141         4139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6198_length_4139_cov_2.841332, whole genome shotgun sequence.
ACCESSION   DAOMVE010000141 DAOMVE010000000
VERSION     DAOMVE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6198_length_4139_cov_2.841332"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLS64_07700"
     CDS             complement(<1..598)
                     /locus_tag="PLS64_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HRL43742.1"
                     /translation="MFSTDKSSFNKKDVSDCDYKISLAGNPNVGKSTIFNELTGLRQH
                     TGNWTGKTVEFARGCCKIKDARFCITDLPGCYSLLSFSGEEAVTRECLIQNQNDCVVI
                     VVDCGVIERNLSFALQVLSVTKNAVLCLNLCDEAEKNGIKIDCDELSLNLGIPVVKTC
                     ATDKKGLDELKKAIYNVCSGKIKCFRVERNFEGVDILSE"
     gene            complement(614..850)
                     /locus_tag="PLS64_07705"
     CDS             complement(614..850)
                     /locus_tag="PLS64_07705"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRL43743.1"
                     /translation="MKEKCSLDKLPLGVTGIVYRVNCNENIKNRIFDFGIMENSSVIP
                     LFTSPFGDPSAYLVKNAVVALRNNDCKDIIVTPE"
     regulatory      1028..1172
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1318..3207
                     /locus_tag="PLS64_07710"
     CDS             1318..3207
                     /locus_tag="PLS64_07710"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRL43744.1"
                     /translation="MNKSFKKILSIVLSVMMISSLMTVSLSVSAVEDGKVRVIVRNDT
                     YSVENGAPWDGVLVDEWVSINNDTTMMSAVADALNNHGYTQEGAENNYISSINGLAAF
                     DGGTMSGWMGTLNDWFTNSGYASYTVADGTLESGDEIAIMYTSNGYGEDIGGTWANND
                     TTVKSVEITGAELSGEFDPSVTDYTLTIDTPSADVNVVPTATNKNFQTRKYKNEYLPS
                     DDSAFYKRSQTVSVSDGDKIIIGCGDTAWPSMNTSEGGTVYTFTVKYAPSAADTVSNK
                     IDEVAKYLASQDAPTVSSVGGEWTVLGLARAGKITDEIADSYYQNAVKYVEEKGSAKL
                     HNTKSTDNSRVILALTAIGKDVTDVASYNLLEPLADMDYVKKQGINGPIFALIALDTG
                     DYEIPQTDAANPTTREKLVQTILDAQVANGGWTFFGSTADPDMTGMAIQALAPYYSTN
                     SDVKEAIDKALTAMSNAQNENGGFASWGSVNSESCAQVLVALTSLGIDPTNDERFIKN
                     GNTLIDAMMSFSAENGFGHTDTTYNQMATEQGFYAFVSFDRLVNGKTSLYNMTDRLAE
                     NYAVGDVNLDNTVSVIDATLVQKQIVNLEQLSKVSLIKADVNHDGVINVVDATEIQKI
                     IVKLV"
     gene            3234..>4139
                     /locus_tag="PLS64_07715"
     CDS             3234..>4139
                     /locus_tag="PLS64_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HRL43745.1"
                     /translation="MEKIKKFVKKNQILLIAVCVCIAALTVAFFAGGNRSENKVQTAS
                     TASSLSTTNADTETTSVSATEKTTQNPTDSAKSNKDKKDRKNSEDKKNSKDNSQNSNS
                     SAVEKSEKSEQSSNNSSSDKKKPSSSSNSSSKPNKKNDVQPTTQDKYKTDPVPSGKPK
                     PVEPEEQETKDTNLYCTFTITCSTILDNMDDLDPEKIELVPKDGIILKKQKVQFKEGE
                     SVYDVLVKVCKDNNIHMESSWTPMYNSAYIEGINNLYEFDCGSLSGWMYKVNEWFPNY
                     GCSRYVLKNGDDVVWCYTCNLGYDVG"
BASE COUNT         1350 a          850 c          803 g         1136 t
ORIGIN      
        1 tttcagatag tatatcaacg ccttcaaaat ttctttctac cctaaaacat tttatctttc
       61 cgctgcatac attatagatt gcttttttta actcatccaa accttttttg tctgttgcac
      121 aggttttcac aacaggtatt ccgaggttta gcgataactc gtcgcagtcg atttttatac
      181 cgtttttttc agcctcatca caaagattaa gacaaagaac agcattcttt gtgacagata
      241 acacctgcaa tgcaaatgaa agatttcttt caataactcc gcagtcaaca actattacaa
      301 cgcagtcatt ttgattttgt ataagacact cccttgttac cgcctcttcg cccgaaaatg
      361 aaagcaggga atagcatccg ggtaagtccg taatacaaaa tcttgcgtct ttgattttgc
      421 aacaacctct ggcaaactca actgttttac ccgtccagtt tcccgtgtgc tgtcttaatc
      481 ccgtcagttc gttaaaaatt gtactcttgc cgacattagg attaccggca agtgaaattt
      541 tgtaatcaca atccgagaca tcctttttat taaaagatga tttatccgta gaaaacaata
      601 aaaaagcctc cttttattca ggagtaacaa tgatatcttt gcaatcgtta tttctcagtg
      661 ccacaacggc atttttaaca agatacgcag acggatcacc gaacggactt gtaaacaagg
      721 ggataacaga actgttctcc ataataccaa aatcaaatat tctgtttttg atattttcat
      781 tacagttcac acgatagaca atccccgtaa ctccaagcgg taacttgtca agcgaacatt
      841 tttctttcat ctgattcacc gatttcaact ttttattaac agcatattca attcatacaa
      901 ctgagttaca caaaaagccg gccataaact aaacttatat cgtacctgaa taaactctga
      961 tatcagaaaa attttaagga attgacacga ctataaacag gtgttataat taaatgcaga
     1021 tttcaaatgt atataactta atatgggttt acggttcaaa atgcatcttg cagagtagat
     1081 aacagagaat tcggtgagaa tccgaagcaa cagtcattac tgtatttggc attgccataa
     1141 gtcaggtcgc tgtccgtatg cctcggattt tgcaaatctt ggacaaggaa gaggtgcagc
     1201 cgaccatatc tgtacagact tttggtctgt atggttttgt ttgttgtgcc ttccaaaaac
     1261 ggagggcttt tttattttgc ctttcgatat acacaataca atatggagga aaacactatg
     1321 aacaaatctt ttaaaaaaat cctctcaatt gtactttctg taatgatgat ttcgtcactt
     1381 atgacggttt cactttcagt aagcgcagtt gaagacggaa aagtaagagt aattgtcaga
     1441 aatgatacat actcggtaga aaacggcgct ccgtgggacg gcgttctcgt tgatgagtgg
     1501 gtaagcatta ataatgacac tactatgatg tcagccgttg ctgatgcact caacaatcac
     1561 ggctacacac aggaaggtgc tgagaacaat tacatctcgt caattaacgg tcttgctgca
     1621 tttgacggcg gtacaatgag cggctggatg ggaactctca atgactggtt cacaaattca
     1681 ggttatgcat cctatacagt agcagacggc acacttgaga gtggtgatga aatcgctatt
     1741 atgtatactt caaacggtta cggcgaggat atcggcggta catgggcaaa taacgataca
     1801 actgttaaat cggttgagat cacaggtgcc gagctttcgg gcgaatttga tccgtcagtt
     1861 acagactaca cactcacaat cgacactccg tcagctgatg taaatgtagt tcctactgca
     1921 actaacaaaa acttccagac aagaaaatat aagaatgaat atcttccttc tgacgacagt
     1981 gcgttttaca agagaagtca gactgttagt gtttctgacg gtgacaaaat tatcatcggt
     2041 tgcggtgata ctgcatggcc gtcaatgaac acaagtgaag gcggtactgt atatacattc
     2101 acagtgaaat atgctccttc cgcagctgac acagtttcaa acaagattga cgaggtcgca
     2161 aaatatctcg catcacagga tgcccccaca gtttcttcag taggcggcga gtggacagtc
     2221 ctcggtcttg caagagcagg taaaatcaca gacgaaattg cagacagtta ctatcagaat
     2281 gcagttaagt atgttgaaga aaagggttct gcaaagcttc acaatactaa atcaacagac
     2341 aattcaagag taatccttgc acttacggca atcggtaagg atgttacgga tgttgcatca
     2401 tataaccttc ttgaaccgct tgcagatatg gattatgtta agaagcaggg tatcaacggt
     2461 cctatctttg cactcattgc acttgatacg ggcgattatg aaattccgca gactgacgcc
     2521 gcaaatccca caacaagaga aaagctcgta cagacaattc ttgatgcaca ggtagcaaac
     2581 ggtggctgga cattcttcgg ctcaactgca gatcctgata tgacaggtat ggcaattcag
     2641 gcacttgctc cttactactc aacaaacagc gatgttaaag aggcaattga taaagcactc
     2701 acagcaatgt caaacgcaca gaatgaaaac ggcggatttg catcatgggg ttctgtaaac
     2761 agcgaaagct gtgcacaggt actcgttgca cttacaagcc ttggaattga tcctacaaat
     2821 gatgaaagat tcatcaaaaa cggcaacaca ctcatcgatg ctatgatgag tttctccgct
     2881 gaaaacggat tcggtcatac agatacaaca tacaatcaga tggcaaccga acagggtttc
     2941 tatgcttttg tttcatttga ccgtcttgta aacggcaaaa cttcactcta caatatgact
     3001 gaccgacttg cagaaaatta tgccgtaggt gatgttaatc ttgacaacac agtttctgta
     3061 attgacgcaa cactcgttca gaagcagatt gtaaaccttg aacagctttc aaaggtttca
     3121 cttatcaaag ctgatgtaaa tcatgacggt gttatcaatg ttgttgatgc aacagaaatt
     3181 cagaaaatca tcgttaagct cgtttgatta aagttcacaa gtaaagattg gttatggaaa
     3241 agataaaaaa gtttgttaaa aaaaatcaaa tacttttaat tgcggtgtgc gtttgtattg
     3301 ccgcacttac agtagcattt tttgcaggcg gcaaccgatc ggaaaataaa gttcaaactg
     3361 catcaactgc ctcctcgcta tcgacaacaa atgcagacac agaaacaact tctgtgtctg
     3421 caacagagaa aaccactcaa aatcctaccg attccgctaa atcaaataaa gataaaaagg
     3481 acagaaaaaa ttccgaagat aaaaagaaca gtaaggataa ttcgcagaac agtaacagtt
     3541 cagctgttga aaaaagtgag aaatccgagc agagttcaaa caacagttct tcggataaga
     3601 aaaaaccgtc ttcttcatca aacagttctt caaaaccgaa caaaaagaat gatgttcagc
     3661 cgacaacaca ggataagtac aaaaccgatc ccgtaccgtc gggaaaacca aagccggttg
     3721 aaccggaaga acaggaaaca aaagatacaa atctttattg tacctttaca atcacctgtt
     3781 caacaattct tgacaacatg gatgaccttg atcccgaaaa aattgaactc gttccaaagg
     3841 acggtataat cctcaaaaaa cagaaagttc aattcaaaga gggtgaatct gtatatgatg
     3901 tccttgtaaa agtttgtaag gacaacaata ttcatatgga atcgagctgg actcctatgt
     3961 ataattccgc ttatattgaa ggaattaaca acctctacga atttgactgc ggttctcttt
     4021 cgggctggat gtacaaagtc aatgaatggt ttccaaacta cggctgttca cgatatgtac
     4081 tcaagaacgg cgacgatgtg gtctggtgct acacttgtaa cttaggctat gatgtcggc
//
LOCUS       DAOMVE010000142         4058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6375_length_4058_cov_2.139395, whole genome shotgun sequence.
ACCESSION   DAOMVE010000142 DAOMVE010000000
VERSION     DAOMVE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6375_length_4058_cov_2.139395"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /locus_tag="PLS64_07720"
     CDS             complement(<1..2028)
                     /locus_tag="PLS64_07720"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRL43746.1"
                     /translation="MTVRAFSDFVVNPESEPTELFRVDKGSSKTINIKVWLQDDKATT
                     EYAGKTVSISGVNIVTQNKKGNKVYFVDRTVNEDIKNWTKGVTFQQGDKTPVTMKFNE
                     NSHTYSCEYTPAAGDTIVKFTSGSVTWSTDMKSLNSGESMYYTAYGDHNSSNAGYGTW
                     GEVIEISVSSKTDVLPDTGNVSSVYMVPDKNDSYSKVRMPFTNDRNKWVGYIAKEKAD
                     NMTFSFTNNGNTYKIPAPNRGNSTLFVVTSATTGYWDPPATITVTAGKNDAGDPKVSY
                     DSLKSTTISVTPGTKVKLIANPKKGFVLKNWVNSDTGAVADGIGSDGSFTPTASGNYN
                     FTAVYVESLTFEAYVRTYDGASLSENTNGGSVEIKCGNQNSTVDSNDGTHITLNAVKG
                     STVTYYAKANDGYVFDGWYTDADCNSVPENLSDKYELANVETSKKLYAKFKVDIYTVK
                     AYAQHGNNPPSGDAGNVSFDNNNYASEVTTTVNRNGEVTFYAKPEKGYAFIGWYATKD
                     AADPKVAVKDCTLNGDVYSTKINIPYSDVKKYALYARFKALYTVEAKAMYNNENVVKA
                     GTVKVGNEKADKISSKPVMEGNDVKVEAIAKKGYKFAGWYTDEACKKPYFKENNNVSP
                     ITLNKVSKGITLYAKFESDSTTIYFYNSNDKWTNVYAYMWENAKGKGNENSA"
     assembly_gap    2153..2162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2163..2773)
                     /locus_tag="PLS64_07725"
     CDS             complement(<2163..2773)
                     /locus_tag="PLS64_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43747.1"
                     /translation="MRERLALLREYLKNRLAFGKNKKTRKGFILTMVALVEVLAISVV
                     SVSAWVETISTITIKAEGAKIDNYVYTNADICSGNGYSGKTIDLTKYFRAAGGVHLAS
                     ASSADGENIFFPIKKSDVSDFGANSYRCADVNDKNVNYIDFSFNVKVDKTFNANHAEF
                     YLDQVPKITIGGADVSGNLVRMAITDTDTQNTVVCGSEASNKNV"
     gene            complement(2802..>4058)
                     /locus_tag="PLS64_07730"
     CDS             complement(2802..>4058)
                     /locus_tag="PLS64_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43748.1"
                     /translation="SSVNSLGSATTTKAKGRSFSDYYFGTGAPLFTLDGLKSQNITMV
                     AWLEATGENCDAYEGQNVSITVELESNWKDMEYVTFVDKTKGDADNDENTELMWVGNA
                     GCFLVMTYYDENFQNPKSVVMSASKTKVNAEGKPQPIEWIAYLPKDKITNISFMRYSK
                     VKEKIKLDGTNEVEVGRIYNVWHTTAEVNDWIKANNSGKGEKAFNWSHQINKEHGLQT
                     YRTDDGTATGNKENTYYAVHGNGYGDTPTVAENVAPCIGYWGTKYPNSSGGSVEPTTT
                     EQQPTTGDAYAEADIVIDNKLWFNDYNWYNGGGGLRNLLSQNVFVLKAVFSDTHGNET
                     AYAMKPESSGDKCKLNRTSVKAGSKLVRFDLYISDTDTKKCEYDVPDTIQHTFVVREG
                     GSSYNISYELVNEGSGKIIKVGNWEN"
BASE COUNT         1125 a          886 c          671 g         1366 t
ORIGIN      
        1 cgcactattt tcatttcctt ttccctttgc gttttcccac atatacgcat agacattcgt
       61 ccatttatca ttagagttat aaaaatagat tgtagttgaa tcgctctcaa acttagcata
      121 cagagtgatt cccttgctaa ctttatttaa ggttataggt gaaacattgt tgttttcctt
      181 gaaatacggt tttttacaag cctcgtccgt ataccaaccg gcaaacttgt aacctttttt
      241 ggcaattgcc tcaactttta catcattgcc ttccataaca ggtttagatg aaattttatc
      301 ggctttttcg ttgccaacct ttacagtacc ggctttaaca acattttcgt tgttgtacat
      361 cgctttggct tctaccgtat acaaagcctt aaatcttgca tacagtgcat atttctttac
      421 atcggaatat ggtatattaa tttttgtaga atagacgtca ccatttaatg tacaatcttt
      481 aaccgctacc ttcggatctg ctgcatcctt agtcgcatac caaccgataa aagcatagcc
      541 tttttcaggc ttagcataaa aagtaacttc tccgttacga tttaccgttg ttgttacttc
      601 agacgcataa ttattattat caaaagatac attaccggca tcaccggaag gaggattgtt
      661 accgtgctga gcgtatgctt ttacagtata aatatcaact ttgaatttcg catacaactt
      721 cttggaggtc tcaacattag ccaattcata tttatcgctc aaattctccg gtacactatt
      781 acagtctgca tctgtgtacc agccgtcaaa aacatatccg tcattagctt ttgcgtaata
      841 tgtaactgtg ctgcccttaa cagcattgag tgtaatgtgt gttccatcgt tggaatctac
      901 agtcgagttt tggtttccac acttaatttc aactgagccg ccatttgtgt tttcagaaag
      961 gcttgcaccg tcatatgttc tgacataagc ctcaaatgtc aacgactcaa cataaacggc
     1021 tgtaaagtta tagttacctg aagctgtcgg ggtaaaagaa ccgtcgctac caatgccgtc
     1081 tgccacggca cctgtatcgc tgtttaccca gtttttcaat acgaaacctt ttttaggatt
     1141 tgctattagc ttaacctttg tacctggggt tacagaaatt gtagtacttt tcaaactatc
     1201 ataactaact ttaggatctc ctgcatcatt cttgcctgca gtaacagtaa tagtagcagg
     1261 tggatcccaa taacctgttg tagcagaagt aacaacaaaa agcgttgaat tgcctctgtt
     1321 aggagcagga attttatatg tattaccgtt atttgtaaaa gaaaaagtca tgttgtctgc
     1381 cttttccttt gcaatataac cgacccattt attcctgtca ttagtaaatg gcattctaac
     1441 tttactgtaa gagtcatttt tatccggaac catatataca cttgaaacat tgcctgtatc
     1501 agggagtaca tcggttttgc ttgaaacact gatttcaatc acttcgcccc atgtaccgta
     1561 tccggcatta ctgctattat gatcgccata ggcagtatag tacatacttt cgcctgaatt
     1621 gagcgacttc atatcagtag accaagtgac acttcccgag gtgaacttaa ctattgtatc
     1681 gccagcggcg ggtgtgtatt cacaggaata tgtatgcgaa ttctcattaa acttcatcgt
     1741 tacaggagtt ttgtcaccct gctgaaatgt tacacccttt gtccaatttt ttatatcttc
     1801 atttacggta cggtcaacaa aatacacttt attgcctttt ttgttctgag ttacaatatt
     1861 aacaccagat attgaaacag ttttaccggc atattcagta gtcgccttat catcctgaag
     1921 ccacacttta atatttattg ttttactgct tcctttatca accctaaaca actcggttgg
     1981 ttcagattca ggattaacaa caaaatcgct aaaagcacga acagtcacag gagtttgatt
     2041 gccctctgct ttactgacaa catttttatt acttgcctgt ctacttattt acgataggtg
     2101 ggagaacaat aacaacaatg caaatataga ggtgttgagg aaggaagtcc aannnnnnnn
     2161 nnacattttt attacttgcc tcagaaccgc atacaaccgt attttgagta tcggtatctg
     2221 taattgccat tcttacaagg tttcctgaaa catctgcacc acctatagtt atcttaggta
     2281 cttgatcaag gtaaaactct gcatgattcg cgttaaaggt tttatcaacc tttacattaa
     2341 atgaaaagtc aatataatta acattcttat cgtttacatc cgcacatctg taagaattag
     2401 caccaaaatc agaaacatcc gatttcttta tcgggaaaaa tatattttca ccgtcagcac
     2461 ttgaagccga agcaagatgc actccacccg cagcacggaa atatttagtc aagtcaatgg
     2521 ttttgcctga ataaccatta ccactgcaaa tatcggcatt agtataaaca tagttgtcaa
     2581 tttttgcgcc ctcagcttta attgtgattg ttgaaatagt ttcaacccaa gccgaaacgg
     2641 acacaaccga tatggcaagt acttcaacaa gtgccaccat agtgagtata aaaccttttc
     2701 ttgttttttt attcttgcca aatgcaagtc tgtttttcaa atactccctt aaaagagcaa
     2761 gtctttctct catcgtgaca cctccttaat gtgttaatca attaattttc ccaattgcct
     2821 acttttatga ttttgccaga tccttcattt accaattcat aggatatgtt ataagaactg
     2881 ccaccttcac gcactacaaa tgtatgctga atggtatcag gcacatcata ttcgcatttt
     2941 tttgtatctg tatcagaaat atacaaatca aatcttacaa gcttcgaacc ggctttaaca
     3001 cttgtcctat ttagtttaca cttatctccg ctgctctccg gtttcattgc ataagcggtt
     3061 tcattaccgt gtgtatcgct aaatacggct tttagtacaa aaacattctg actaagcaga
     3121 tttctcagac ctccgccgcc gttataccaa ttataatcat taaaccataa tttgttatca
     3181 attacgatat ccgcctctgc atatgcatct ccggttgtcg gctgttgttc ggttgtagtc
     3241 ggttcaacac tgccacctga actgttggga tactttgtac cccaatatcc gatacagggg
     3301 gcaacatttt cagcaacagt aggggtatcg ccgtaaccgt taccgtgtac ggcataatag
     3361 gtattttctt tatttccggt tgcagttccg tcatctgtgc ggtatgtctg aagaccgtgt
     3421 tctttattta tttgatgtga ccaattaaac gctttttcac ctttgccact attatttgct
     3481 ttaatccaat catttacttc agctgttgta tgccatacat tatatattct gcctacctca
     3541 acctcattag ttccgtcaag cttaattttc tcttttactt tactatatcg cataaaagat
     3601 atgtttgtta ttttatcctt tggcagataa gctatccatt caataggttg tggttttccc
     3661 tcagcattaa ccttagtctt tgacgcactc attactactg actttggatt ttgaaagttt
     3721 tcgtcataat aggtcataac aaggaaacat ccggcattgc caacccacat gagttccgta
     3781 ttttcgtcat tgtcagcatc accttttgtt ttgtcaacga aagtaacata ttccatatct
     3841 ttccagttac tttcaagttc aactgtaatt gacacattct gaccctcgta agcgtcacag
     3901 ttctctccgg ttgcctcaag ccaagcaacc atggttatgt tttgggattt aagaccgtca
     3961 agagtaaaaa gaggagcacc tgtgccgaaa tagtagtcgg aaaatgacct gccctttgct
     4021 ttagtggttg ttgccgagcc aagcgaatta acagagct
//
LOCUS       DAOMVE010000143         4057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6378_length_4057_cov_2.785107, whole genome shotgun sequence.
ACCESSION   DAOMVE010000143 DAOMVE010000000
VERSION     DAOMVE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6378_length_4057_cov_2.785107"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLS64_07735"
     CDS             complement(<1..317)
                     /locus_tag="PLS64_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43749.1"
                     /translation="MKKRIYCITTALCLVLCIVLCIAFAAVCSKAENSKTLLPEVRNN
                     GTAIQWRDTAEEEWHDLVALNTLRGTDGKNGKDGKDGTDGKDGIDGKNGLNGKDGTNG
                     KDGL"
     gene            complement(314..916)
                     /locus_tag="PLS64_07740"
     CDS             complement(314..916)
                     /locus_tag="PLS64_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL43750.1"
                     /translation="MRTTEEQHNERKREIMEKCFECYAENGLTGTGIKALAAACGCTT
                     GNLYSYFNSVDELIIESTAYCMEKVEDDFMEKAPTDPKDVVRFVKEVPYWTAKKHGKK
                     YRLMYQVYTHPKYIEHGKKFFEGVNERYTEYAKRLEPKIGIPYTVITPLIFIFVRACV
                     HYAMFEDEYYLKTQMEVLKQGVALFADKYRSQYLRGGNDK"
     gene            complement(1016..2158)
                     /locus_tag="PLS64_07745"
     CDS             complement(1016..2158)
                     /locus_tag="PLS64_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL43751.1"
                     /translation="MKKLSLQWRITLMTALLIGATCIFMNVLIGYSGSHYMDSIGSYV
                     TGYGDTDKGEPDFFDPEHEKLDRELTIVINGAQESFIATNWYITAAATLLGGVLAYFL
                     SGHALKPLRTFTARVEKVQPNNLTDMKITEEVPPELRQFVKSFNRMLDRLDEGFTAQR
                     QFTGNAAHELRTPLSLMQAQLELFSAEHPEVSPETAEFLRLLREQTERMTQMTKTLLE
                     MSELRTVSCADRIEIGPMVEEIFTDLAPLAEKNNVTLESTGDAVMTGSDTLIYRLLYN
                     LTENAIRYNRFDGTVNITVTHKDNQLVITVADTGYGIPEQYRESIFQPFFRVDKSRSR
                     EYGGVGLGLSLVWEIVALHNGKVRVEESSEKGTVIAVKFPTDVIKI"
     gene            complement(2155..2832)
                     /locus_tag="PLS64_07750"
     CDS             complement(2155..2832)
                     /locus_tag="PLS64_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015565319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL43752.1"
                     /translation="MRILVIEDERALCETIVRSLKRLAYSVDFCYDGNKANELLGIEK
                     YDLVLLDLNLPGADGMTVLRTLRQTDRDTGVLILSARCEVADKVEGLDAGANDYLTKP
                     FHLEELEARIRSLTRRRFTQNNVVLNCGKVAFDTKARVAVADGQELSLTRKEIGILEY
                     LLLNQGRPVSQEELLDHVWDNSVDNFSNSIRVHISALRKKLRAALGYDPIRNRIGEGY
                     VMEEEKV"
     gene            2987..3133
                     /locus_tag="PLS64_07755"
     CDS             2987..3133
                     /locus_tag="PLS64_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009260783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HRL43753.1"
                     /translation="MTRKKKAVFQIVLIICGIMMLCYGALRGEAETVLSKAIRLCLEC
                     VGIG"
     gene            3126..>4057
                     /locus_tag="PLS64_07760"
     CDS             3126..>4057
                     /locus_tag="PLS64_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRL43754.1"
                     /translation="MDNKEPKKKRGGVSQFLSRFRWLIQAGATLLSNIHLPNLLKGRI
                     YQGKGKAVCVPGLNCYSCPAASGACPIGSFQAVVGSSKFSFSYYITGFLILIGVLLGR
                     FICGFLCPFGWLQELLHKIPTKKLSTKKLKPLTYIKYAVLLFAVGLLPMLITNDVGMG
                     DPFFCKYLCPQGVLEGAIPLSAVDSGIRAALGTLFSWKLGILIAVIVLSVLFYRPFCK
                     WLCPLGAFYALLNKVSLFGMKVDKHKCISCGKCAKACKMDVDVTKTPNHTECIRCGKC
                     IRACPVEAVSFCYGFGKGKENDCPAKKAETAKQK"
BASE COUNT         1057 a          949 c          860 g         1191 t
ORIGIN      
        1 agaccgtctt ttccgttagt accgtcttta ccgttaagac cgttctttcc atcaatgcca
       61 tctttgccgt cagtaccgtc tttgccgtcc ttgccgtttt ttccgtcagt acctctgagc
      121 gtgttaagag ctacaagatc gtgccattcc tcttcggcgg tatctctcca ttggattgct
      181 gtgccgttgt ttcgcacctc cggcaacagg gttttgctgt tttccgcctt gctgcatacg
      241 gcagcaaatg caatgcaaag tacgatgcaa agcacaagac agagtgcggt agtaatacag
      301 tatattcgtt ttttcattta tcattgcctc ctctcaaata ttgtgaacgg tatttatcag
      361 caaacagggc aacaccctgt tttaagacct ccatttgggt ttttaaataa tattcatcct
      421 caaacatagc atagtgtaca caggcacgga caaatataaa aatgagcggt gtaatcaccg
      481 tgtacggaat gccgattttc ggctcaagcc gcttggcata ctcggtatag cgctcattta
      541 ccccctcaaa gaatttcttg ccgtgctcaa tatatttcgg atgggtatag acctgataca
      601 tcagccgata tttcttgccg tgcttctttg cggtccaata cggaacctct ttcacaaatc
      661 gaacaacatc cttcgggtcg gtgggagctt tttccataaa atcatcctcg accttttcca
      721 tacagtatgc agttgattca ataatcagtt catcaacact gttgaaatag gaatacagat
      781 ttcccgttgt acagccacac gctgccgcca aagccttgat gcccgtaccg gtcaatccgt
      841 tttcagcata gcattcaaag catttttcca ttatttccct cttacgctcg ttgtgctgtt
      901 cctctgttgt tctcataaag ttcgcttcct tttgataact aatcgattaa ttaattatag
      961 tatagcacag agctacggta atttcaacaa ttaattaaga aaaaatcgca aaatattaaa
     1021 tttttatcac atctgtcgga aactttactg ctataacagt acccttttcc gaactttctt
     1081 ccacacgaac tttaccattg tgcagtgcaa cgatttccca caccaaagaa agtcccaatc
     1141 cgactccgcc gtattctctg cttcgggatt tatccacacg aaagaagggc tgaaaaatac
     1201 tttctctgta ttgttcgggg atgccgtagc cggtatccgc aaccgtaata acaagctgat
     1261 tgtctttgtg cgttacggta atattcactg taccgtcaaa acggttgtag cgtatagcgt
     1321 tttcggtcag attgtataac aaacggtaaa tcagcgtgtc actgcctgtc ataacagcat
     1381 cgcccgtgct ttcaagtgta acattgttct tctctgcaag gggcgcaagg tcagtgaaaa
     1441 tctcttctac cataggacct atctctatgc ggtcggcaca cgatactgtc cgcagctcgc
     1501 tcatttccaa cagggttttt gtcatttgcg tcattcgttc ggtctgctca cgcaacaatc
     1561 ggagaaactc cgccgtttca ggcgatacct cgggatgctc tgccgaaaaa agttcaagct
     1621 gtgcctgcat aagcgagagc ggcgtgcgca actcgtgtgc ggcgtttccc gtgaactgtc
     1681 gttgagcggt aaatccctca tcaagacggt caagcatccg attgaacgac ttgacaaact
     1741 gtctcagttc aggcggaact tcttcggtta tcttcatatc cgtcaaattg ttaggttgaa
     1801 ccttttcaac ccgagcggta aaagtacgca gaggcttcag cgcatgcccg cttaaaaaat
     1861 acgcaagcac accgcctaac aatgttgccg ccgccgtaat ataccaattg gtagcgataa
     1921 aggattcctg tgctccgtta attacaatag tcaattcacg atcaagcttt tcgtgttcgg
     1981 gatcgaaaaa gtcaggttct cctttgtccg tatctccgta acctgtaaca taagagccta
     2041 tggaatccat ataatggctg ccggaatagc cgataagaac attcataaaa atgcaggtgg
     2101 ctccgataag aagtgcagtc atcagagtta tgcgccattg caaggatagc tttttcatac
     2161 tttttcttcc tccataacat atccttcacc aatgcgattg cgaatcgggt catagccgag
     2221 tgccgcacgc agtttctttc gtaatgcaga aatatgtact cttattgaat tgctgaaatt
     2281 gtcaacactg ttatcccaaa catggtcgag cagttcttcc tgactgacgg ggcgaccttg
     2341 attaagaagt aaatattcaa gtattccgat ttcttttcgg gtaagtgaca gctcctgacc
     2401 gtcagcaaca gcaacacggg ctttggtgtc aaaagcaacc tttccacagt tcaaaacaac
     2461 attgttttgt gtaaaccgcc ggcgagtcag actgcgtatt cttgcctcaa gctcctccaa
     2521 atgaaaaggc tttgtcaggt aatcgtttgc tccggcatcc agtccctcca ccttgtcggc
     2581 aacctcacag cgtgccgaaa gaattaacac tccggtatct ctgtcggttt gtcgcagagt
     2641 tcgcagtacc gtcatcccgt ctgcaccggg taaattcaga tcaagtaaaa caagatcgta
     2701 cttttctatt ccgagcaact catttgcttt gtttccgtca taacaaaaat caacgctgta
     2761 tgccaatctt ttaaggctgc gaacaattgt ctcgcacagc gcacgctcgt cttcaattac
     2821 taaaatacgc atagaaactc tccttgttgt gttttcatat ttctttgata atttaattat
     2881 atcattatat cgctcagaca taaagttttt gttaagtttt cgttcttaac agggatttta
     2941 catcctctgt gctacaatga gagcacaaaa gaaaggagtg accgaattga ctcgaaagaa
     3001 aaaagccgta tttcagatcg tactgattat ctgcggaatt atgatgctgt gttacggagc
     3061 tctgcgtgga gaggcagaaa ccgtgttaag caaggcaatc cggctgtgtt tggagtgtgt
     3121 gggaattgga taacaaagaa ccgaagaaaa aaagaggcgg tgtatcgcag tttctctcac
     3181 gctttcgatg gcttattcaa gcaggagcaa ctttgctgag taatattcat ctgccgaatt
     3241 tattgaaggg cagaatttat caaggcaaag gaaaggctgt ttgcgttccg ggactgaatt
     3301 gctattcctg ccctgcggca tcgggtgcat gcccaatcgg ctcgtttcag gctgtggtgg
     3361 gttcgtcaaa attcagtttt tcatattata tcacaggatt cctcatattg ataggagttc
     3421 tgctcgggcg ttttatctgc ggttttttgt gtccgttcgg atggcttcag gaactgttac
     3481 ataagatacc gacaaaaaag ctttccacta aaaagctgaa gccgctgaca tacataaaat
     3541 atgcagtttt gctttttgct gtaggtttgc tgccaatgct cattacaaac gatgtgggta
     3601 tgggagatcc ttttttctgt aagtatcttt gtcctcaagg ggtacttgag ggggcaatcc
     3661 ctctttccgc cgtcgattcg ggaatccgtg ccgcactggg tacgctcttt tcgtggaagc
     3721 tgggcatatt gattgcggta atcgtactga gtgtactttt ctacagaccg ttctgcaagt
     3781 ggctgtgtcc tctcggtgca ttttatgcat tgcttaacaa ggtgtcactt ttcggaatga
     3841 aagtagacaa acacaagtgc atttcctgtg gaaaatgtgc caaagcatgc aagatggatg
     3901 tagatgtaac caaaactccc aaccataccg aatgcatacg ctgcggtaag tgtatccgtg
     3961 cctgtcctgt cgaggcggtc agcttttgct acggctttgg caaaggaaaa gaaaatgatt
     4021 gtcccgctaa aaaagcggaa acagcaaaac aaaaaat
//
LOCUS       DAOMVE010000144         4019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6466_length_4019_cov_3.951060, whole genome shotgun sequence.
ACCESSION   DAOMVE010000144 DAOMVE010000000
VERSION     DAOMVE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6466_length_4019_cov_3.951060"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="PLS64_07765"
     CDS             <1..940
                     /locus_tag="PLS64_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRL43755.1"
                     /translation="IILGLLISYGTGLVLYFISQANPDLIQNVPWLFSGGADANGVYQ
                     PIFDFTSLNTICDNISKGHIFGSEGLIGIPIHWDKTVFGGIDYSNGALIASSIIAIVP
                     IAFATMMEHIGDICAISSTTGNNYIKDPGLHRTLVGDGLATTLASLFGGPANTTYGEN
                     TGVLALTRVYDPRVIRIAAYFAVAVSFFPIVSVIIGSIPSCIIGGISFVLYGMISAIG
                     VRNVVENKVDFTKSRNLIVAAVILVCALGLSSDTVSFTIGSAAITLSPLAVASIAGIV
                     LNAVFPGKDYKFDTEDVADAVNFEKEVKPKEKKEKK"
     gene            complement(1237..2904)
                     /gene="ilvB"
                     /locus_tag="PLS64_07770"
     CDS             complement(1237..2904)
                     /gene="ilvB"
                     /locus_tag="PLS64_07770"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRL43756.1"
                     /translation="MQLTGAEIICECLLEQGVDTVFGYPGGAALNTYDALYKYSDKIR
                     HILTAHEQGASHAADGYARATGKTGVVMTTSGPGATNIVTGLATANIDSIPLVAITGN
                     VTTDQLGRDSFQEVDIVNIVKPVTKASFLVEKVEDLADTIRKAFALAQHGRKGPVLVD
                     VPKDVTANKTEFVQTIPERPRTFEIRNPEKVRVVQEMIKNAKRPMIYAGGGIISADAS
                     EEFKAFAELIDAPVCCSLMGLGCIPADHPLCVGNIGMHGGYETGMATAECDLMIACGA
                     RFSDRVAGDREKFGEQAKIVQLDIDEKEINKNVKVDEYILGDIKEILIALTIGLDRQN
                     HPDWLAKIEEWKHHFDDKKLPECDLPLPQQVLDTINEIKCPSDIIATDVGQHQMWVAQ
                     FSKIMESRTLLTSGGMGTMGYGMGAAIGAQCARPNDRVCLITGDGSFHMNLNELVTLK
                     SYELPVVVVVMNNTVLGMVRQWQKLFYGSRFSQTDPHRATDFVKLANAFGVDGLRITK
                     PEEIKPILTKAFEMNKPVVVDCHISPDANVLPMIPPGKTINEIITEM"
     gene            complement(3065..3760)
                     /locus_tag="PLS64_07775"
     CDS             complement(3065..3760)
                     /locus_tag="PLS64_07775"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF004079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRL43757.1"
                     /translation="MTGIICAMQIEADGIIALSQNTQTEEIAGMKFTTGTLNGKDIAV
                     VVCGVGKVNAAMCAVVLIEKFNPDVVINSGVGGSLSPLVSIGDIVVATKSVQHDMNTT
                     ALGDRQGEVSFPDGTKIFFDCDKTVVEKLTQACRALPDTKVEQGIIASGDIFVSDRKK
                     RKRIADTFGALVCEMEGAAIGYVCYALKTPYGIIRAISDDLDENKGVDYEKFCKLASK
                     KTVGAICEYLKNE"
BASE COUNT         1017 a          917 c          896 g         1189 t
ORIGIN      
        1 gattatcctc ggtttgctta tctcttacgg tacaggtctt gtgctttact tcatttcaca
       61 ggcaaatccc gaccttattc agaatgttcc gtggcttttc tcgggcggtg ctgacgcaaa
      121 cggtgtttat cagccgattt tcgactttac aagcctgaac acaatttgcg acaacatctc
      181 aaaaggtcac attttcggca gtgagggact tatcggaatt ccgattcact gggataaaac
      241 ggtattcggc ggtattgatt actcaaacgg tgcgcttatc gcatcttcaa tcattgccat
      301 tgttccgatt gcttttgcta caatgatgga gcatatcggc gatatttgcg ccatcagttc
      361 aacaacaggc aataactaca tcaaagatcc gggactccac agaactcttg tcggtgacgg
      421 ccttgcaaca acacttgcat cacttttcgg cggccctgca aacacaacat acggtgaaaa
      481 cacaggcgtt ctcgctctta cgagagttta tgacccgaga gtaataagaa ttgccgctta
      541 ctttgccgtt gcagtatcat tcttcccgat tgtttcggta attatcggtt caattccaag
      601 ctgtatcatc ggcggtattt cgtttgttct ttacggtatg atttctgcta tcggcgtcag
      661 aaatgttgtt gaaaacaagg ttgacttcac aaagagccgt aaccttatcg ttgccgcagt
      721 tattcttgtt tgtgcgctcg gtctttcaag cgacacggta agctttacaa tcggcagtgc
      781 cgcaataaca ctttcgccac ttgctgttgc atcaattgca ggtatcgttc tcaacgcagt
      841 tttccccggt aaggactata agttcgatac ggaagatgtt gccgatgccg taaattttga
      901 aaaagaagtt aaaccaaagg aaaagaaaga aaagaagtaa ttttacttta atagtcgtaa
      961 cacaaaacag ccgtattttt acggctgttt ttttacgctt tgtttaaagc tatgtattca
     1021 aagttgaacg ccgctactga atggcataaa aaacagggta ctcgaacgag taccctatat
     1081 atgtttaatt attaagttag ctgtgttcgg aaatatgcag atgttattta atcaaactac
     1141 ctacacgaac aaggaacagc ggttttctgt gctcgggaac tgaaagctgt tccaaacaca
     1201 aactgcctac acgaactgcg tgccgaggct ctcggcttac atttcggtga tgatttcgtt
     1261 gattgttttt cccggaggaa tcatcgggag aacatttgca tcgggactga tgtggcagtc
     1321 aacaacaacg ggcttgttca tctcaaaagc ctttgtgaga atcggcttga tttcctcggg
     1381 ctttgtgatt cttaaaccgt caacgccgaa agcgtttgca agctttacaa agtcggttgc
     1441 tctgtgcgga tcggtctgtg aaaaacgact gccgtagaaa agcttctgcc actgacgaac
     1501 catgccgaga accgtgttgt tcataacaac tacaacaacg gggagttcat aggacttcag
     1561 tgttacgagt tcgtttaagt tcatgtggaa actgccgtca cctgtgatga ggcaaaccct
     1621 gtcattggga cgggcacact gcgcaccgat tgcggcgccc atgccgtaac ccatagtacc
     1681 cataccgccc gatgtgagga gcgttctgct ttccataatc ttgctgaact gtgcaaccca
     1741 catttggtgc tgacctacat ctgttgcgat gatgtctgac ggacacttga tttcgttgat
     1801 tgtatcaaga acctgctgtg ggagcggaag gtcgcactcg gggagctttt tatcgtcaaa
     1861 atgatgcttc cattcttcaa tctttgcaag ccagtccgga tggttctgac ggtcaagacc
     1921 gattgtgagt gcaatgagaa tttcttttat atctccgaga atgtattcat caacctttac
     1981 attcttgtta atttcttttt cgtcaatgtc aagctgaaca atctttgcct gttcgccgaa
     2041 cttttcacgg tcgcctgcaa cacggtcgga gaatcttgca ccgcacgcaa tcataaggtc
     2101 acattcggct gttgccatac ctgtttcgta accgccgtgc ataccgatgt tgccgacgca
     2161 gagcggatgg tcggcaggaa tacagccgag tcccataagc gaacaacaaa cgggagcgtc
     2221 aataagctct gcaaatgcct tgaattcttc gcttgcgtca gcgctgatga taccgccgcc
     2281 tgcgtaaatc ataggacgct ttgcgttttt aatcatttcc tgaacaacac gaactttttc
     2341 ggggtttctg atttcaaagg ttctcggtct ttcgggaata gtctgtacaa actctgtttt
     2401 gtttgccgtt acatcctttg gaacatcaac gagaacaggt ccctttctgc cgtgttgagc
     2461 aagagcaaaa gccttgcgta tggtgtcggc aagatcctca accttttcaa caaggaagct
     2521 tgcctttgta acgggcttta cgatgtttac aatgtcaact tcctgaaagc tgtctcttcc
     2581 aagctgatcg gttgttacat tgcctgtgat tgcaacaagc ggaatgctgt cgatattggc
     2641 tgttgcaaga cctgttacaa tgtttgttgc accgggaccc gatgttgtca taacaacgcc
     2701 tgttttgcct gttgctcttg cgtaaccgtc agccgcatga gaagcaccct gttcgtgtgc
     2761 tgtgagaatg tgacgaattt tgtcgctgta tttatagagc gcatcgtatg tgttgagtgc
     2821 cgcaccgccc ggataaccga aaaccgtgtc aacaccctgt tcaagaagac actcgcaaat
     2881 gatttctgca cctgtaagct gcatatatat ctctcccttg tatatgtagt gttattccat
     2941 aaaacaatgt taccttttat tttatccaaa tgtgattata ttgtcaatag aaatcgaaaa
     3001 agacaactca ttacaaaaga aaaaacgaga aacaaaagcg ttccccgttt tgagttggtc
     3061 ataattattc atttttgaga tattcacata ttgcgcctac ggtttttttg cttgcaagtt
     3121 tgcagaattt ttcatagtca acacccttgt tttcgtcaag gtcatcggag attgccctga
     3181 tgattccgta aggagttttg agtgcgtagc aaacataacc gattgccgca ccctccattt
     3241 cacagacaag agcaccgaat gtgtcggcaa ttcttttgcg ttttttgcgg tcggaaacga
     3301 atatgtcgcc gcttgcgata atgccctgct caaccttggt gtcggggagt gccctgcacg
     3361 cctgcgtgag cttttcaact actgttttgt cgcagtcaaa gaatatcttt gtaccgtcag
     3421 gaaatgaaac ctcgccctgt ctgtcgccga gagcagttgt gttcatatcg tgctgaacgg
     3481 attttgtcgc aacaacaata tctccgattg atacaagagg ggagagtgaa ccgcccacac
     3541 cgctgttgat tacaacatcg ggattgaact tttcaataag cacaacggca cacatggcgg
     3601 cattgacctt gccgactccg cagactacaa cggctatgtc cttgccgtta agagtacctg
     3661 tggtgaattt cattcctgcg atttcttcgg tttgtgtgtt ctgagaaagg gcgataattc
     3721 cgtcggcctc aatctgcatg gcacatataa ttcctgtcat aaattttcct ccgattttgg
     3781 tgttgaaatg tagcggcgtt cagcgaacgc caatcgtttt ggttgcaaaa ccaaaacgaa
     3841 caagcgataa atttatgttg tatcattgtc ggttaaaaat catttgtaat gtcgaaccga
     3901 ttttgtaatc ggttcgattg gcggtcactg accgccgcta ctgtttgcat aaatccatac
     3961 aatattgtgc tcgggtgaca attgttatca tatataaaac tgtcttcacg agaggggtg
//
LOCUS       DAOMVE010000145         4012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6477_length_4012_cov_4.129896, whole genome shotgun sequence.
ACCESSION   DAOMVE010000145 DAOMVE010000000
VERSION     DAOMVE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6477_length_4012_cov_4.129896"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..918)
                     /gene="pflA"
                     /locus_tag="PLS64_07780"
     CDS             complement(163..918)
                     /gene="pflA"
                     /locus_tag="PLS64_07780"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRL43758.1"
                     /translation="MSDNAVKGFVHSIETFGSVDGPGVRFVIFLSGCAMRCQFCHNPD
                     TWKHDENTVEYTADELLNKALAYRPYWKNKGGITVSGGEPLLQIDFLLDLFKKAKKQG
                     VNTCIDTSANPFTTKEPFYSKWKELMEYTDLLLLDIKHIDNEQHKILTGQPNTNILEM
                     ARQLSDMGKPVWIRHVLVPERNDKDEYLFRLADFIRSLKNVERVEVLPYHTLGVYKWK
                     ALGYDYPLEGINPPTKERVKNAEMILNGEKTRD"
     gene            complement(918..3155)
                     /gene="pflB"
                     /locus_tag="PLS64_07785"
     CDS             complement(918..3155)
                     /gene="pflB"
                     /locus_tag="PLS64_07785"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HRL43759.1"
                     /translation="MREEWTNFAGGKWESDIDVRDFIQRNYTPYDGDQSFLEGPTEAT
                     DKLWGELKELQKEERAKGGVLDMETEVVSGITAYGAAYLDKDLEKVVGLQTDKPLKRA
                     FMPYGGIKMAEQSCQMYGYTPSPKLHEIFTKYHKTHNQGVFDIYTPEMKLARHNKILT
                     GLPDTYGRGRIVGDYRRVALYGIDRLIEGKKEDFAETNRQGMRRGDFQLREEIADQIR
                     ALNDMKEMALSYGFDISQPAKNAAEAVQWLYFGYLAAIKTQNGAAMSVGRVSTFLDIY
                     IERDLENGTLTESQAQELVDHFVMKCRMVKFARIESYNQLFSGDPVWATLEVAGLGID
                     GRSMVTKNDYRFLHTLENMGPSPEPNLTVLYSSRLPENFKKYAAHISVTTSSIQYEND
                     DVMRPVWGDDYSICCCVSATQTGKEIQFFGARANLAKCLLYAINGGIDEKTKMQVGPE
                     YTPITSEYLDYDEVIKKYDTMMDWLAHLYVGTLNMIHYMHDKYYYEAAEMALIDTDVR
                     RTFATGIAGFSHVVDSLSAIKYAKVKVIRDEDGIAVDFETEGDFPRYGNNDERADKIA
                     VDLLKTFMAKLKYCHTYRDSEPTTSILTITSNVVYGKATGSLPDGRKAGEPLSPGANP
                     SYGAEQNGLLASLNSVAKLPYEYALDGISNTQTINPGALGHNDDERVDNLVNVMDGYF
                     DQGAHHLNVNVFGTEKLIDAMEHPEKEEYANFTIRVSGYAVKFIDLTREQQLDVIART
                     CHDRM"
     gene            complement(3422..3913)
                     /locus_tag="PLS64_07790"
     CDS             complement(3422..3913)
                     /locus_tag="PLS64_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRL43760.1"
                     /translation="MKTDRVITAMIEYFGSDKKRIHHFLKVYSFAKTIGESENLLPDD
                     QELLEISAIVHDIGIKVSEEKYNSSAGKYQELEGPHEAEKLLAALGYEKTFIDKVCYL
                     VGHHHTYGNIDTLPYRILVEADFLVNLYEDDSSRSAAEQAYDKIFRTNTGKNLLKIMF
                     LIP"
BASE COUNT         1063 a          904 c          851 g         1194 t
ORIGIN      
        1 tacgctgaga tttctcaaca aggacagctt aaaaatcttt gaatacacct ttgattcatc
       61 aattttgctg aagaaaaaac aacaaaagca gaataacaaa taaaaatcaa aacaaaaacg
      121 gcagtaccaa aaaaatcggt actgccgttg ttatataaga aattaatctc ttgttttttc
      181 gccgttaaga atcatttccg cattcttaac acgctctttt gtcgggggat taatgccctc
      241 gagaggatag tcataaccga gagccttcca cttgtacaca ccgagggtgt gatacggaag
      301 cacctctaca cgctcaacat tcttgagcga acggataaag tcggcaagtc tgaaaagata
      361 ctcgtcctta tcatttcttt caggcacaag aacatgtctt atccatacgg gcttgcccat
      421 atcggaaagc tgtcttgcca tttcaagaat attggtgttt ggctgtcctg taaggatttt
      481 gtgctgttcg ttgtcgatat gctttatatc aagcaacagc aaatccgtat attccattag
      541 ctctttccac tttgaataga acggctcttt ggttgtaaac gggttggcgc ttgtgtcaat
      601 acaggtatta acgccctgct ttttagcctt cttgaacaaa tcaagcaaaa agtcaatctg
      661 taaaagcggc tcacctccgc ttactgtgat accgccctta tttttccaat aggggcggta
      721 tgcaagcgct ttattaagca actcgtcggc ggtgtattcc acggtgtttt catcgtgttt
      781 ccaggtgtcg ggattgtggc agaactggca acgcattgcg caaccgctca gaaaaatcac
      841 aaatctcaca cccggaccgt caacagagcc aaaagtttct attgagtgta caaagccttt
      901 aacagcgttg tccgacatta cattctgtcg tggcaggttc tggcaataac atccagctgc
      961 tgctctcttg taaggtcgat gaacttaaca gcataacccg atacacggat tgtgaagtta
     1021 gcgtactctt ccttctcagg atgctccata gcgtcaatga gtttttctgt accgaataca
     1081 tttacattga ggtggtgtgc gccctgatca aagtagccgt ccataacatt tacgaggttg
     1141 tcaacacgct cgtcatcgtt gtgtccgaga gcacccgggt tgattgtctg agtatttgaa
     1201 ataccgtcaa gagcgtactc gtaaggaagc ttagcaacag agttaagtga agcaagaaga
     1261 ccgttctgct ctgcaccgta gcttgggtta gcacccggtg aaagcggctc gcctgctttt
     1321 ctgccgtcag gaagtgaacc tgtagcctta ccgtaaacaa catttgatgt gattgtaagg
     1381 atagaagttg taggctctga atctctgtat gtgtggcaat acttgagctt agccatgaat
     1441 gtcttgagaa gatcaacggc aatcttatct gctctctcgt cattgttacc gtatcttggg
     1501 aagtcgccct ctgtttcaaa gtcaacagca atgccgtcct cgtcacggat aaccttaacc
     1561 ttagcgtact taatagcgct gagtgagtca acaacatgtg agaagcctgc aatacctgtt
     1621 gcaaatgttc ttcttacatc tgtatcgata agagccattt ctgctgcctc atagtagtac
     1681 ttatcatgca tgtagtgaat catgttaagt gtgcctacat agaggtgagc aagccaatcc
     1741 atcattgtgt catacttctt gataacttcg tcataatcaa ggtactctga tgtgataggt
     1801 gtatattcag gaccaacctg catctttgtc ttttcatcaa taccgccgtt gatagcgtag
     1861 agaaggcact tagcaaggtt agctcttgcg ccgaagaact ggatttcctt acctgtctgt
     1921 gttgcggata cgcagcagca gattgagtaa tcatcgcccc atacaggacg cattacatca
     1981 tcgttctcgt actgaattga gcttgttgta actgaaatat gagcagcata cttcttgaag
     2041 ttctccggaa gtcttgaaga ataaagaact gtgaggtttg gctcaggtga aggtcccata
     2101 ttctcaagtg tgtggaggaa acggtagtcg ttctttgtaa ccattgaacg gccgtcaatg
     2161 ccaagacctg caacttcgag tgtagcccat acaggatcgc ccgagaagag ctggttgtaa
     2221 gactcaattc ttgcaaactt aaccatacgg cacttcataa caaagtggtc aacgagttcc
     2281 tgagcctgtg attctgtaag tgtaccgttc tcaaggtcac gctcgatgta gatgtcgagg
     2341 aatgttgata cacgaccaac gctcattgca gcgccgttct gagtcttgat agcagcaaga
     2401 taaccgaagt agagccactg aactgcctct gcagcgttct tagcaggctg tgaaatatca
     2461 aagccgtaag aaagtgccat ttccttcata tcgttaagag cacgaatctg atcggcgatt
     2521 tcttcacgaa gctggaagtc gcctcttctc ataccctgac ggtttgtttc tgcaaagtcc
     2581 tctttcttgc cttcgataag acggtcaata ccgtaaagag caactcttct gtagtcaccg
     2641 acgattctgc ctctgccgta tgtgtcggga agacctgtaa gaatcttgtt gtgacgggca
     2701 agcttcatct caggagtgta gatatcgaat acaccctggt tgtgagtctt gtgatatttt
     2761 gtaaagattt cgtggagttt agggcttgga gtgtagccat acatctggca tgactgctca
     2821 gccattttga taccgccgta aggcataaat gcacgcttga gaggcttgtc tgtctgaaga
     2881 cctacaacct tttcaaggtc tttatcaaga tatgcagcgc cgtaagctgt gatgccgctt
     2941 acaacctcag tttccatatc aagaacgccg cccttggctc tttcttcctt ctggagttcc
     3001 ttgagctcgc cccagagctt gtctgtagct tctgtaggac cttcaaggaa agactggtcg
     3061 ccgtcataag gtgtgtagtt tctctgaatg aagtctctga catcaatgtc ggactcccat
     3121 ttgccacctg caaaatttgt ccattcttct ctcattactg taaaacctcc gataatgata
     3181 attaatactg aattttgtat atgacacaaa aaaatgccga ctcaattcca agcagacgcc
     3241 cggtaaggtc agctccttga aaaaatcgtc atacatcctt gtattctgtc atttattcac
     3301 ttttataatc atataaccaa caccattaaa ataacacttt ttaagggcgt tgtcaagcat
     3361 tttgagatac tttttacacc actttttcac actcaaagca ctatggtaaa tgagtacaat
     3421 tttagggtat gaggaacatt attttcagca gattttttcc tgtatttgtg cggaatattt
     3481 tgtcatatgc ctgctctgct gcactgcggc ttgaatcgtc ctcataaagg ttcacaagga
     3541 agtctgcctc taccaaaatt cggtacggca aagtatcaat atttccatag gtgtgatggt
     3601 gtcccacaag gtaacaaact ttgtcaataa atgttttttc gtagccgaga gcggcaagca
     3661 acttttcagc ctcgtgagga ccttcaagtt cctgatactt tcctgcagac gaattgtact
     3721 tttcttcgct cacctttatg ccgatgtcgt gaacgattgc ggagatttca agaagctcct
     3781 gatcgtcggg caaaagattt tcactttcac cgattgtctt tgcgaatgaa taaaccttga
     3841 gaaaatggtg aattcttttc ttgtcacttc caaaatattc aatcatagca gttataaccc
     3901 tgtcggtttt catattatca ctccttacgg cgagccgccg taccctattc gtgtagacag
     3961 tttttttgtt tgtgcaattc tgtatgggta gcattgcgac tatatttcag ac
//
LOCUS       DAOMVE010000146         3941 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6655_length_3941_cov_3.481987, whole genome shotgun sequence.
ACCESSION   DAOMVE010000146 DAOMVE010000000
VERSION     DAOMVE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_3941_cov_3.481987"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..927)
                     /locus_tag="PLS64_07795"
     CDS             complement(172..927)
                     /locus_tag="PLS64_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43761.1"
                     /translation="MKKSLVLIVAAILMLSLFGCSDKKNADVSGVVDAPDKFSTLDEA
                     KVTENPNIRKESENVNGMCYAITLEEFTYNYNEMLKEAGNLDMINPKGWKMLGSPQND
                     ANGTEYQYYYYNADKINFTASVETKTNYIMNIGLGTTMSNFVSIENEKNNSDTILMKS
                     AVMAAAVSGMGINKVDTIQDIFYLTTFENTNELWYNGNVYALSTNEDKADSERSTMLF
                     RTFPISDEVKENWGIDEYESYVANATQVTTNAN"
     gene            complement(997..3567)
                     /gene="gyrA"
                     /locus_tag="PLS64_07800"
     CDS             complement(997..3567)
                     /gene="gyrA"
                     /locus_tag="PLS64_07800"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRL43762.1"
                     /translation="MDNEQNQNKNSRIIDVDLQNEMRKSFLDYSMSVIVSRALPDVRD
                     GLKPVHRRILYTMNQIGLDPSKPYHKCADTVGQVLGSYHPHGDASVYDAMVRLAQDFS
                     MRYMLVDGHGNFGSVDGDPPAAYRYTEARMSKISLEMLTDIEKNTVDFMSNYDDRLKE
                     PTVLPSRFPNLLVNGSSGIAVGMATEIPPHNLGETIDAVCTLIDNPDAELAEIMECMP
                     GPDFPTGGIIMGRSGIRAAYATGRGKITVRAKTEIIEAKNGRYKIIVTELPYKVNKAR
                     LIENIADLVKDKRIEGISNIEDHSDRKGMHIEIDIKRDASPQIVLNQLFSYTQLQTTF
                     GAIMLSIVDGEPKILSLKEMLQCYIEFQAEVIRRRTDFDLKKARDRAHILEGLKIALD
                     FIDEVIKIIRNSKDTASAKAGLMERFGLDDVQAQAIVQMRLGQLTNMEQTKIEDEIAA
                     LHAKIEEYLEILASEPRQLEIVKEEIMQIRNKYADPRRTEICAVSGEVDIEDLIPQEE
                     CVLTLTQFGYVKRLSADTYKIQRRGGRGVSGMSRREEDIAAEMFVINSHDYVLFFTDK
                     GRVYRLKCYEVPEGSRQSKGVNIANLIPISPDEKVTSMIRVPEFDEEKYLVMVTRQGI
                     IKRIELNAYNTSRKGGLIALELNEGDELAWVRMTDGNSQVIIATKKGMAIRFNETDIR
                     AMGRQARGVKAMALKEGDCVVGMSVVREGGLVLTVSETGYGRLSSPDDYRVQSRGGKG
                     LTNYHTEKYGDVAAIKVVNLDDDIIIISEEGIIIRIAAESIRVCARPSKGVTVMKVNG
                     DDRVVTIARVPHIDGEDDESAESEDNAENGEPSEAVTEEVSENNEGETSDSTEENTEE
                     "
BASE COUNT         1016 a          884 c          791 g         1250 t
ORIGIN      
        1 cttccgttat cggcggaata aagcattcta tcgcactgcg aagcttggga tgacgctcct
       61 caggcaacgg ctgttgacag aaagaaaaag taaaacaaat aacagaagag attaaactat
      121 aaggcggcta tcgaaaacga tagtcgcctt gtctttttgc aaaattcatt tttaatttgc
      181 gtttgttgtt acctgagttg cgtttgccac atagctttca tactcgtcaa tgccccagtt
      241 ttcttttact tcatcagaga taggaaaggt gcgaaacagc attgtactgc gttcactgtc
      301 tgccttatcc tcattggtgc tgagagcata gacattgccg ttgtaccaca gctcatttgt
      361 attttcaaag gttgtaagat agaaaatatc ctgaattgta tcaaccttat taatgcccat
      421 tccgcttact gcggcagcca ttacggcact tttcatcagg attgtatcgc tgttattttt
      481 ctcgttttca atcgaaacaa agttgctcat tgttgttccg agaccgatat tcataatata
      541 atttgtcttt gtttcaacag aggctgtgaa gttgatttta tcggcattgt agtaataata
      601 ctgatactcc gttccgtttg catcattctg aggactgccg agcattttcc aaccctttgg
      661 attaatcata tcaagattac ccgcctcttt gagcatttcg ttgtaattat acgtaaattc
      721 ttcaagagtg attgcgtagc acattccgtt tacattttca ctttctttgc gaatgttcgg
      781 attttctgtt acctttgcct catcaagagt agaaaattta tcgggcgcat ctactacacc
      841 cgaaacatcg gcattcttct tgtcagaaca gccgaaaagt gaaagcatga ggattgccgc
      901 tacaatcaga actaaagatt ttttcatcaa atcaccagat tttcatatgt gtcaaaaact
      961 ccacttaaaa aagtggagtt ttcttatttt tatcaattat tcttccgtat tttcttcggt
     1021 tgaatcagaa gtttcaccct cgttattttc ggaaacttct tctgtaaccg cttctgacgg
     1081 ttcgccgttt tctgcgttat cttcgctctc ggctgattca tcatcttcac cgtcaatgtg
     1141 aggaacacgg gcgattgtta caaccctgtc gtcaccgttt actttcatta cggttacacc
     1201 ctttgaagga cgggcgcaaa cacgaattga ttcggcggca attctgataa taataccttc
     1261 ttctgagatg ataataatat catcgtcaag gttaaccacc ttgatagcgg caacatcgcc
     1321 gtatttttcc gtgtggtagt tggtaagacc tttaccgcca cggctctgaa cacggtaatc
     1381 atcgggactt gaaagtctgc cgtagcctgt ttcggaaact gtgagaacaa gaccgccctc
     1441 acggacaaca ctcattccga ctacgcagtc gccctctttg agagccattg ccttaacacc
     1501 tcgagcctgt ctgcccattg cacgaatatc ggtttcgtta aagcgaatag ccataccctt
     1561 cttggtggcg ataataacct gactgttgcc gtcggtcatt cttacccatg caagctcgtc
     1621 accctcgtta agttcaagag cgataagacc gccctttctt gaggtgttgt atgcattgag
     1681 ttcaatacgc ttgataatgc cctgacgggt gaccataaca aggtattttt cctcgtcaaa
     1741 ttcaggcact cttatcattg aggttacctt ttcgtcgggt gaaatcggga tcagatttgc
     1801 aatattcaca ccctttgact gacggcttcc ctcgggaact tcatagcatt taaggcgata
     1861 aactctgccc ttgtcggtaa agaacagaac ataatcgtgt gaattgatta cgaacatttc
     1921 agccgcaata tcttcttcac ggcgtgacat acccgataca ccacgacctc ctctgcgctg
     1981 aattttgtag gtatcggcag aaagacgctt tacatatcca aactgtgtaa gtgtaagaac
     2041 gcactcctcc tgcggaataa gatcctcaat atcaacctca ccgctgacag cacaaatttc
     2101 tgttcttctc gggtcggcat atttattacg aatctgcata atttcttctt ttacgatttc
     2161 aagctgtctt ggctcgcttg caagaatttc gagatattct tcaatctttg cgtggagggc
     2221 tgcaatttca tcctcaatct tagtctgctc catatttgtg agctgtccga gacgcatctg
     2281 aacgatagcc tgagcctgaa catcatcaag accgaaacgc tccataagac ctgcttttgc
     2341 gcttgcagta tccttgctgt ttctgataat tttgattacc tcgtcaatga agtcaagcgc
     2401 aatcttcaag ccctcaagaa tgtgagctct gtcacgggct tttttgaggt caaaatcagt
     2461 acggcggcga ataacctcag cctgaaattc aatatagcat tggagcattt ccttgagtga
     2521 aagaatttta ggctcgccgt caacgataga aagcataatt gcaccgaagg ttgtctgcaa
     2581 ctgtgtgtat gagaacagct ggttaagaac aatctgaggt gaggcatctc tcttgatgtc
     2641 aatttcaatg tgcataccct tacggtcgga atgatcctcg atatttgaaa taccctcaat
     2701 tctcttatcc tttacgaggt cggcaatgtt ttcaataagt cttgctttgt tgaccttgta
     2761 cggaagctcc gtaacgataa ttttatatct gccgttttta gcttcgataa tttcagtctt
     2821 ggctcttacg gtgatttttc ctcgacctgt tgcataggcg gcacgaatac cgcttctgcc
     2881 catgataata ccgcctgtcg gaaagtcggg tccgggcata cactccataa tctctgcaag
     2941 ctcggcatcg ggattgtcaa tgagagtaca aactgcgtca attgtttcac cgaggttatg
     3001 cggaggaatt tcggttgcca taccaacggc aataccgctt gaaccgttta cgagaaggtt
     3061 tggaaaacgg cttggaagaa cagtcggttc tttaagacgg tcatcgtagt ttgacataaa
     3121 gtcaaccgtg tttttctcga tatccgtgag catttcaaga gaaattttac tcattcttgc
     3181 ctctgtataa cggtaagcag caggcggatc accgtccacc gaaccgaagt taccgtgacc
     3241 gtcaacaagc atatatctca tggaaaaatc ctgtgcaaga cgaaccattg cgtcataaac
     3301 cgaggcgtca ccgtgagggt gatacgaacc aagcacctga cctaccgtgt cggcacactt
     3361 gtggtacggc tttgacggat caagaccgat ttgattcatt gtatagagta ttcttctgtg
     3421 aacaggtttc aaaccgtctc tgacatcggg caacgcacgg gaaacaataa cgctcatgga
     3481 gtagtccaaa aagctttttc tcatttcatt ttgcagatcg acatcaataa ttctcgaatt
     3541 cttgttctga ttctgttcgt tatccatatt ctttcctcgc ttttcaagct cttgttactg
     3601 tctgttcaat aatttataat aatgttattt tgcgtttttt agggtggttt agtgtgtgat
     3661 tttgtttcag taggggcgtt cattggacgc ccgccttgat gaaacgattt gttttacaca
     3721 tattttttga tgtgatgaaa agtgtgtatg aattttgtag cggcaaaccg tgttcgccat
     3781 tcattttgct tgaaaaagca aaatgattaa acaacatttt ttatttaaca tcattaccga
     3841 tttaaacaaa tcatttgtat tgccaagtag gttttgcaat tgttttgatt ttgtaccttt
     3901 attcaattcg tgtttacaca cgaattgatt ggcaatcact g
//
LOCUS       DAOMVE010000147         3910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6731_length_3910_cov_2.975616, whole genome shotgun sequence.
ACCESSION   DAOMVE010000147 DAOMVE010000000
VERSION     DAOMVE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6731_length_3910_cov_2.975616"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..802)
                     /locus_tag="PLS64_07805"
     CDS             complement(134..802)
                     /locus_tag="PLS64_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRL43763.1"
                     /translation="MNKDFPRIITFLRKERGLSQKQVAGDMGISQALLSHYEKGIREC
                     GLDFLVKTAEYYGVSTDYLLGRTVQRNFTADPFEAAEKEVVINTEKGSMINKINRSLL
                     MNTTSVIYDLLAKIGNKHLTNAVSNYLMSAEYVIFRTIYNSEKNNSQTMFSLDKNRYR
                     NLTDASMILDLEIINELIENDKLTLTLSPDVISVCFEKDAPSLFNLIQYSERNIKNVY
                     AGKR"
     gene            1001..1627
                     /locus_tag="PLS64_07810"
     CDS             1001..1627
                     /locus_tag="PLS64_07810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRL43764.1"
                     /translation="MKSYIIHFIRHGSIDDTLAGKYIGTTDAPLSDRGRMDLRKLDYE
                     YKYPGTQVVFTSPLKRCTETAKILYPEQNPLVIDNLSECNFGEWEGKTADELKNDPDF
                     KKWLAGDSSVKPPRGESNADFTRRVCKMFESIVEGLMKTGTTESVIVTHGGVIMTLLA
                     VYGLPQAKPFEWTMDNGFGYSLRVTPVLWQRDKVCEVFQTIPLPSEND"
     gene            1729..2799
                     /gene="leuB"
                     /locus_tag="PLS64_07815"
     CDS             1729..2799
                     /gene="leuB"
                     /locus_tag="PLS64_07815"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRL43765.1"
                     /translation="MADYKITLLKGDGIGPEIVNQAVKVLDCAAEKFGFGVTYDEALL
                     GGCAIDATGVPLPDETVAKCKASDSVLLGAVGGPKWDSQPGNNRPEAGLLGIRGALGL
                     FANLRPSVIFGPLKSASPIKDEIIGGNLDVMIVRELTGGIYFGERGRKEVNGQPAAWD
                     TEMYTIGEVERIARVAFDLAMKRNKKLTSVDKANVLESSRLWRETVKRVAEDYPEVEL
                     NHMYVDNCAMQLVRNPRQFDVILTSNIFGDILSDEASMIAGSIGMLASASLSGSKLGL
                     YEPIHGSAPDIAGQNIANPLATILSVAMMLRYSLDQSEAADAIEAAVAKVLETYRTPD
                     IYEEGFTKVGCDEMGDRVCEAL"
     gene            2803..3534
                     /locus_tag="PLS64_07820"
     CDS             2803..3534
                     /locus_tag="PLS64_07820"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43766.1"
                     /translation="MNGMQLFDMHSHILPGIDDGAKTVEDSLELLSCLKNQGVTNVCL
                     TPHFYTNEISLEDFIAERAEKFEKFKPHIPNGMNIVLGTEVYVTKYLFNNENIKGINY
                     GNSNYILTEFPYNATFSDNSYEMLDRLMSEQGLIPVIPHVERYSFLVDNPDVIEDLKR
                     LGVVIQTNISNYTKKAPMMKRRRMLKLVSAGLIDIIGTDAHSFNHNTPELYSDAIECI
                     AKKCGQQAVDRLMKNSEKIFNAAIG"
     gene            complement(3688..>3910)
                     /locus_tag="PLS64_07825"
     CDS             complement(3688..>3910)
                     /locus_tag="PLS64_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HRL43767.1"
                     /translation="ELIKGKSINDALKLTNKAVMEALDGLPPVKVHCSVLAEQAIKAA
                     VSDYYKRRGVDPEPIVGNVPECDSCGCEG"
BASE COUNT         1123 a          758 c          881 g         1148 t
ORIGIN      
        1 aacggtcggt tcagcttgag aaaaactcca agccaaccga ccgttgtttg ttgtgttact
       61 ttacttctat gttacattat aagcgtatat gtagcacgaa agtaaactta ttatcctgaa
      121 aagcagttaa aaatcacctt tttcctgcat agacattttt tatgtttctt tcggaatatt
      181 gaattaggtt gaatagtgac ggggcatctt tttcaaaaca taccgaaatg acatcgggag
      241 agagggtgag ggtaagctta tcattttcaa taagttcgtt gatgatttca aggtcaagaa
      301 tcattgatgc gtccgtgaga tttctgtagc ggttcttatc aagcgaaaac atagtttgcg
      361 agttattttt ttcggaatta taaatcgtgc ggaaaattac atattcggcg ctcataaggt
      421 agttgctgac tgcgtttgta aggtgcttgt tgccgatttt tgcaagcaaa tcgtagatta
      481 ccgaggtggt gttcatcaaa agactgcggt ttattttgtt gatcatactg cctttttcgg
      541 tattgatgac aacctctttt tcggcagcct caaaaggatc ggctgtgaaa tttctctgta
      601 cggttctgcc gagaagataa tcggttgaaa caccgtagta ttcggctgtt tttacgagaa
      661 aatcaagacc gcattcacgg attccctttt cataatgtga caaaagtgcc tgcgaaattc
      721 ccatatcgcc tgcaacctgt ttttgactca aacctctttc tttacgaaga aatgttataa
      781 ttctcggaaa atctttgttc aagacttgac acctcatttt taaagatagt ataattaact
      841 tgattgtcag aaaaagcaaa gcagaatttg ctctaataat atatagtgca ttgaaagcat
      901 ttacagcaat atactttcta tacagacagt ataatacaaa atttactttt tgtaaataat
      961 taagtgccga tttgctgaaa gtcggtttat gggagaagtt atgaaatcat acataattca
     1021 ttttataaga cacggttcga ttgatgatac acttgcgggg aaatatatcg gaacaacaga
     1081 cgctccgctt tccgacaggg gcagaatgga tttgagaaaa ctcgattatg agtacaaata
     1141 tccgggtacg caggttgtgt tcacaagtcc gttaaagcgt tgtaccgaaa ccgcaaaaat
     1201 tctttatccc gaacaaaatc cgcttgttat cgacaacctt tccgaatgta atttcggtga
     1261 atgggagggc aaaactgccg atgagctgaa aaacgatccc gattttaaaa agtggcttgc
     1321 aggcgattct tccgtaaaac ctccgagggg ggagagcaat gccgatttta cacgcagagt
     1381 atgcaaaatg tttgaaagca ttgttgaggg acttatgaaa acaggcacaa ccgaaagtgt
     1441 aattgtcact cacggcggtg ttattatgac cttgcttgca gtctacggac ttccgcaggc
     1501 aaaaccgttt gaatggacaa tggacaacgg tttcggctat tcactcagag ttactcccgt
     1561 actttggcag agggataaag tctgtgaagt ttttcagacg attccgcttc cgtcggagaa
     1621 tgactgattt tttgaaactt tggcagaatt gtagcacagt ttacaatctt ttgccgtata
     1681 ttgtgggata atatccattg ataaacaatt gattaaggag atataaaaat ggcagattat
     1741 aaaattactc ttttaaaggg cgacggtatc ggtcctgaaa tcgtaaatca ggctgtaaag
     1801 gttcttgatt gtgccgctga aaaatttggt tttggcgtta catatgacga ggcacttctc
     1861 ggcggttgtg ctattgacgc aacaggcgtt ccgctccccg atgaaacagt tgcaaagtgt
     1921 aaggcatcgg acagcgtgct tctcggtgcg gtaggcggtc ctaagtggga ttcacagccg
     1981 ggtaacaacc gtcccgaggc aggacttctc ggtatcagag gcgcactcgg acttttcgca
     2041 aatcttcgtc cgtcggttat tttcggaccg ctcaagagtg catcacctat caaggatgaa
     2101 attatcggcg gcaacctcga tgttatgatt gttcgtgagc ttacaggcgg tatctacttt
     2161 ggcgaaagag gcagaaaaga agttaacggt cagcctgcgg cttgggatac ggaaatgtac
     2221 acaatcggtg aggttgagag aattgcaaga gttgcttttg accttgctat gaagagaaat
     2281 aaaaagctca caagcgttga taaggcaaat gttcttgagt cttcaagact ttggagagaa
     2341 acagttaaga gagttgccga ggattatccc gaggttgagc ttaaccatat gtatgttgac
     2401 aactgcgcta tgcagcttgt aagaaatccc cgtcagtttg atgttattct cacatcaaat
     2461 atcttcggtg atattctttc tgacgaggca tcaatgattg ccggctcaat cggtatgctc
     2521 gcatctgcaa gcctttcggg cagcaagctc ggtctttatg agcctatcca cggttctgct
     2581 cctgacattg cgggtcagaa tattgcaaat ccgcttgcta caattctttc tgttgcaatg
     2641 atgctccgtt attcacttga tcagagcgag gcggcagacg caattgaggc agctgttgca
     2701 aaggttcttg aaacctaccg tacgcccgat atctatgaag agggtttcac aaaggttggc
     2761 tgtgatgaaa tgggcgaccg tgtttgtgag gcactttaat tggtgaacgg tatgcagctt
     2821 ttcgatatgc actctcatat tcttccgggt atagatgacg gtgcaaaaac ggttgaggac
     2881 agtctggagt tgctctcctg tcttaaaaat cagggagtaa cgaatgtttg tcttacacct
     2941 catttttata caaatgaaat cagccttgaa gattttattg ccgagagggc ggaaaagttt
     3001 gaaaaattca aaccgcacat tccgaacggt atgaatatcg ttctcggaac agaggtttat
     3061 gttacaaagt atcttttcaa caatgaaaat atcaaaggta taaactacgg aaattcaaac
     3121 tatattctca ctgagtttcc gtataatgca acattttcgg acaattcgta tgaaatgctc
     3181 gacaggctta tgagcgaaca gggacttatt cccgtaattc ctcatgttga gcgttacagc
     3241 tttcttgttg acaatcctga tgtgattgaa gatttaaaac ggctcggtgt tgttattcag
     3301 acaaatattt caaattacac aaagaaagct ccgatgatga aacgccgcag aatgttaaag
     3361 cttgtaagtg caggacttat cgacattatc ggtacggacg cacattcatt caatcataat
     3421 acacctgagc tttattccga tgcaatcgaa tgtattgcca aaaaatgcgg tcagcaggcg
     3481 gttgacagat tgatgaaaaa cagcgaaaaa atcttcaatg ccgcaatcgg ctgatatatt
     3541 gcaaaaataa taatcacaca aataaaaaac gattgatttc ctttcggatt tcaatcgttt
     3601 ttttgttcaa ctgattctac ggtataatcg ggagcacaaa aatgcccgac ttttacgcag
     3661 tcgggcagat gtatgatttt aagttaatca gccttcacag ccacagctgt cacattcggg
     3721 aacattgccc acgattggct cgggatcaac gcctcttctt ttgtagtagt ccgaaacggc
     3781 tgccttgatt gcctgttctg caagaacaga gcagtgaacc ttgacaggcg gaagtccgtc
     3841 aagagcctcc ataaccgcct tgtttgtgag cttgagagcg tcattgattg acttgccctt
     3901 gataagctct
//
LOCUS       DAOMVE010000148         3833 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6922_length_3833_cov_3.017999, whole genome shotgun sequence.
ACCESSION   DAOMVE010000148 DAOMVE010000000
VERSION     DAOMVE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6922_length_3833_cov_3.017999"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLS64_07830"
     CDS             complement(<1..253)
                     /locus_tag="PLS64_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRL43768.1"
                     /translation="MTAPIYYQSDCNLDLLKGKKIAIIGYGSQGHAHALNLKDSGCDV
                     IIGLYKGSKSWAKAEAQGFKVYTAAEAAKQADIIMILIND"
     gene            complement(335..826)
                     /gene="ilvN"
                     /locus_tag="PLS64_07835"
     CDS             complement(335..826)
                     /gene="ilvN"
                     /locus_tag="PLS64_07835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005356988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRL43769.1"
                     /translation="MQYTLSILVENQAGVLSKISGLFSRRGFNIDSLAVGVTQDPSVS
                     RITIVANGDEYIVEQLEKQLNKLIPVIKVKRLNEGEFISRELVLIKVHCAASKRAEIV
                     KTVEIMQAKIVDVSPSSVTVEYCECASRINTLVDLLKPYGIREIVRTGTVAIDRGTAS
                     VSV"
     gene            complement(839..2461)
                     /gene="ilvB"
                     /locus_tag="PLS64_07840"
     CDS             complement(839..2461)
                     /gene="ilvB"
                     /locus_tag="PLS64_07840"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRL43770.1"
                     /translation="MISGAEIMVKCLEAEGVDIVFGYPGAAICPFYDKVYDSSITHIL
                     VRQEQNAAHAASGYARCSNRPGVCVATSGPGATNLITGIATAYTDSIPIVAITGQVRS
                     DLLGRDVFQEADITGACEPFVKHSYLVSKTEDLPRVFKEAFHIASTGRPGPVLIDIPV
                     DIQENEIESFEYPESVNIIGYKPNTKGHAIQVKKAVEAINASKRPVIVSGGGVLSSGI
                     KLKLQKFAETTGIPVISTMMGIGVMPSEHELYLGMLGTHGKAVANRALHEADLVIVCG
                     ARLGDRAVAAPDQMSENTKVIHIDIDPAEIGKNVKTEIPIVGDMKNVINMLLDSIGDY
                     KVTTMWSETVKNWKKDLYREPPVFDGFVEPRTLVGHLSAMFRSKAILVADVGQNQIWC
                     ANNFRIREGRFLTTGGMGTMGYSIPAAIGAKFARPDRDVIVICGDGSFQMGLNELATI
                     AGNQLGIKIIIMKNARLGMVRELQDKHYGGRHSATILNGDPDFLKIADAYGIDHAEAH
                     SNKEAENIIKGIVDSEKPFILVCDVHPDTPSI"
     gene            complement(2658..>3833)
                     /locus_tag="PLS64_07845"
     CDS             complement(2658..>3833)
                     /locus_tag="PLS64_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43771.1"
                     /translation="SSSSNNPTEQTTVTDTDDLSLDSDIETTELNHDFEIYNNGKKVD
                     TLNLYGLFKQIDNGFIYSKINKDSPNEMEYYRYYLDKKNSVKLGSIKNWSMQINDKAY
                     VNNHLCFTASTGDITDEENSTLEFIDIDLNKNKMTTVFSDKGGFPYDTVTGADNLVII
                     TKVLDNGTSLELYDTETQKFKQLKYLEFDDKNSIGETIRKVSIDKSTKTISLLVLDCI
                     SQNEASLKIETYDYDMNFLKSYDISNISDDSNELIQGVQVFDFKNNYLFYQNFSITRC
                     IGYIDSDKLKKSDISEDVDDTFSIVSASEDDSDTNLLYKGAESSSENNYIYLFDTTNK
                     TMKETKFNIEEKGYTIGGISRIGKDNLMIMMSPDNADKKSDLNSRIYFTKLSDLNFQ"
BASE COUNT         1028 a          789 c          766 g         1250 t
ORIGIN      
        1 catcgttgat aagaatcatg atgatgtcag cctgctttgc agcctctgct gctgtatata
       61 ccttaaagcc ctgtgcctca gcctttgccc agctcttgct gcctttgtaa agaccgatga
      121 ttacatcgca acctgaatcc ttgaggttga gagcgtgtgc atgaccctgg ctgccgtaac
      181 cgataatagc aattttctta cccttgagaa ggtcaaggtt acaatctgac tgatagtaga
      241 ttggtgctgt catgtttttt gtgtagtctt tcatattaaa gtcctcctta ccgcaaacgc
      301 ggcatataaa ttaaaatata tgctaactgt aagattaaac ggaaaccgaa gctgtgcctc
      361 tgtcaatggc aacagtacct gttcttacaa tctcacggat accataaggc ttgagtaagt
      421 caacaagcgt gttgatgcgg cttgcacatt cgcagtactc aacggtaact gacgaagggg
      481 atacatcaac gattttcgcc tgcataattt caaccgtttt gacaatttcg gctctttttg
      541 aggcggcgca gtgaaccttg attaaaacaa gctcacggct gataaattcg ccctcgttaa
      601 gcctttttac cttaataacg ggaatgagct tgttgagctg tttttcaagc tgttcaacaa
      661 tgtactcgtc accgtttgca acgattgtga ttcgggaaac cgacgggtcc tgagtaacac
      721 ctactgcgag tgagtcgatg ttaaaacctc ggcgagagaa aagtcccgaa atttttgaaa
      781 gaacgcccgc ctgattttca acaagtattg ataatgtata ctgcatttta tcacctcatt
      841 aaattgacgg agtgtcgggg tgaacatcgc aaacaagaat gaacggtttt tccgaatcaa
      901 caataccctt gatgatgttt tcggcttcct tgtttgaatg agcctctgca tggtcaatgc
      961 cgtaagcatc ggcaatcttc aagaagtcgg gatcaccgtt gagaattgtt gcggagtgtc
     1021 tgccgccgta gtgtttgtcc tgaagttcac gaaccattcc gagtcgtgcg tttttcataa
     1081 tgatgatttt aattccgagc tgattgcctg caatagtcgc aagctcatta agccccatct
     1141 gaaaactgcc gtcaccgcag attacaataa catcacggtc gggtcttgca aactttgcac
     1201 cgattgcagc gggaattgag taacccattg tacccatacc gcctgttgtg aggaatctgc
     1261 cctcacgaat acggaagttg tttgcacacc agatttgatt ttgtcctaca tcggcaacga
     1321 gaattgcctt tgagcggaac attgcgctga gatgaccgac aagtgttctc ggctcaacaa
     1381 agccgtcaaa aacgggaggc tcacggtaaa ggtctttttt ccagttcttt acggtttcgc
     1441 tccacattgt tgtaaccttg taatcgccga tgctgtcaag cagcatattg atgacatttt
     1501 tcatatcgcc tacaatcgga atttctgttt taacattctt accgatttca gcagggtcaa
     1561 tgtcaatgtg aattaccttt gtgttttcac tcatctggtc gggagccgca acagctctgt
     1621 caccgagtct tgcaccgcat acgattacaa ggtcggcttc gtgaagagca cggttggcaa
     1681 cagccttgcc gtgagtgccg agcattccga gataaagctc atgctcgctg ggcataacac
     1741 cgatgcccat catagttgaa ataacgggga tacccgtagt ttctgcaaat ttttgaagtt
     1801 taagcttgat acccgaactc agcacgccgc cgcctgatac gataacagga cgcttgcttg
     1861 cgttgatggc ctcaacagcc tttttaacct gaattgcgtg tcccttagtg ttcggcttgt
     1921 aaccgataat gtttacgctt tcgggatatt caaaactttc aatttcattt tcctgaatat
     1981 caacgggaat gtcgataagt acaggaccgg gtctgccggt tgaggcaata tggaaagcct
     2041 ctttgaaaac tctcggaagg tcttctgttt tgctgacgag ataactgtgc tttacaaacg
     2101 gttcacaagc tcccgtgatg tcagcttcct gaaagacatc tctgccgaga aggtcggaac
     2161 gaacctgacc tgtaatggca acaatcggaa tagagtccgt ataggcggtt gcaatgcctg
     2221 tgatgaggtt ggttgcaccg ggaccggaag ttgccacgca aacaccggga cgattactgc
     2281 atcttgcata gccgcttgcc gcatgagcag cgttctgttc ctgacgaaca agaatgtggg
     2341 taatgctcga gtcgtaaact ttatcgtaaa atgggcagat agccgcaccc ggataaccaa
     2401 agactatgtc aacgccctcg gcttccaaac attttaccat aatttcagca ccgctgatca
     2461 ttatgttttg tccctcctat cctttttatt gtgagcaaaa ggtgttttgt aacattaaat
     2521 ataccttttt gctttaccgc taattataca ataaatacct taaaaagtaa agagcagtat
     2581 tgcccataaa ttaaattttt tgcaggattt tgacagataa tttgcaaaat ggattttaat
     2641 tgatttttac cgcctgttta ctgaaaattt aagtcgctta gttttgtaaa gtatattctt
     2701 gaattaaggt cacttttttt gtctgcatta tcaggggaca tcattatcat caaattatct
     2761 tttcctatcc gtgatatacc gcctatagtg tacccttttt cttcaatatt aaattttgtt
     2821 tctttcattg ttttgttggt tgtatcaaaa agatatatgt agttattttc tgaacttgat
     2881 tccgccccct tatacaataa atttgtatca ctgtcatctt cagatgcgga tactattgaa
     2941 aaagtatcat ctacatcttc tgatatgtcg gattttttca gcttatcgct gtctatatat
     3001 cctatacatc ttgttatgct gaaattttga tagaatagat aattgttttt aaaatcaaaa
     3061 acctgtacgc cctgaattaa ttcgtttgaa tcatctgaaa tgttggaaat atcatagctt
     3121 ttgagaaaat tcatatcata gtcataagtt tctattttta aagaagcttc gttctgtgaa
     3181 atgcaatcca aaaccaaaag agagattgtt tttgtacttt tgtcaattga aacttttctt
     3241 atagtttccc cgatactgtt tttatcatca aactctaaat attttaattg cttgaatttt
     3301 tgagtttcgg tatcatacag ttccaaagaa gtaccgttgt ctagcacttt tgtgattatg
     3361 acaagattgt ctgctcctgt aacagtgtca tatggaaatc cgcctttgtc agaaaataca
     3421 gtcgtcattt tgtttttatt aaggtcgata tcaataaatt caagagtact gttttcttcg
     3481 tctgtaatgt cgcccgtgct tgccgtaaag caaaggtgat tatttacata tgccttatca
     3541 ttaatttgca tagaccagtt ttttattgag ccgagtttta cactgttttt tttatctaaa
     3601 taatatctgt agtattccat ttcatttgga gagtctttat tgatttttga atatatgaag
     3661 ccgttatcaa tttgtttaaa taagccgtaa agatttaaag tatccacctt tttgccgtta
     3721 ttgtaaattt caaaatcatg atttaattct gttgtttcaa tatctgaatc aagagataaa
     3781 tcatctgtat ccgttacggt tgtttgttca gtggggttat tgcttgatga aga
//
LOCUS       DAOMVE010000149         3808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_6989_length_3808_cov_3.918465, whole genome shotgun sequence.
ACCESSION   DAOMVE010000149 DAOMVE010000000
VERSION     DAOMVE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6989_length_3808_cov_3.918465"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLS64_07850"
     CDS             <1..238
                     /locus_tag="PLS64_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43772.1"
                     /translation="LADTWMVGKYVGDNALAAVARLPLRVYGSCILLAQLSEKITNNY
                     KWLQFTKIQLSRADYYTHFPHSFQQLLKKNGNAC"
     gene            346..2139
                     /locus_tag="PLS64_07855"
     CDS             346..2139
                     /locus_tag="PLS64_07855"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HRL43773.1"
                     /translation="MKKIINKKSLKGTLAVMLCAIMAFSVCACDFDSDTEPTKTDAQI
                     QFDKYCDDLFSEELEDDALTAHFDISNPSDYGLKYDEEDYTLGHVSDEDTKESFDELK
                     KAKTDLEEFDRSGLTSSQKQTYDTLESYFEIQLSYEGTTELQSIFAPQSGIVANLFTT
                     LSEFTFYEKDDTDLYLAVLKDTKRYMDECIEFTRKQAEDGYFMAEDIAQQSIDECEKH
                     IKNDKSVLVDEFESRIKSLGLSDSEKKTVVETNKKYFEEYYIPALKSANSALESLKKS
                     GKNEEGLCGYGKIGKKYYSAIVKDKTSSSMTPEELKSYLTNSFTKVGMSMSNVSQDDL
                     SKFQDYKPDFKDADEVLEFLIENIEEDFPTPVTTSYTADYMSDSAKSDNVGAYYVQGR
                     IDDTSVNIIKINPDFANKGMTQMYTTLAHEGYPGHLYQFTASNANKDIPNVRKILSFI
                     GATEGWAQYASKCTLDYLDTSEGIKKLIYANDILGYILYSMVDVGVNYNGWDYEKVKE
                     YMSTALGSADSESVTAATKEAYDLARSNPGYFLPYTVGYLKMIDMRETAESELGSSFD
                     AKEYHTFIMNLGITSIDVYEKALDMWLESKK"
     gene            2304..3635
                     /locus_tag="PLS64_07860"
     CDS             2304..3635
                     /locus_tag="PLS64_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL43774.1"
                     /translation="MGVKTENKMAYEPVNKLIIQMSAPPLISMFFQYSYNLVDSMFVA
                     GINEQALAAVSLAFPITTLMNALSVWIGVGVNVLIAGYLGQKNQQKANSAAALGVVIS
                     VVVGVLINFLSLFIINPYYSAFTQNKEIFEYGIAYMSVCVFMQIPNMVHIVIQKILQA
                     TGNMIAPMWFQIAGVVFNFVFDPLLIFGIGPFPKMGVVGAAVATVLGYVVSTIIALIQ
                     LFLTKQKVKPVFKNCLEPQMIKLIFSYGLPSFVMNALGAFTVNFANMFLVRYSDTAVA
                     FFGAYFKVQQLIFMTVNGLIQGCLPIMRFNYRAKEYGRLKQANKSGTIIATVMMIVGM
                     LLVLIFPKEILFLFSASEEMCAFGVPAMRIMSLGFVFSGFSAMIATYEQATDSIMPGV
                     VVQLLRQGVLLVPVIWLLNSAVQMTGIWLAFPITEFVTFLVAVVFAKIKNH"
BASE COUNT         1146 a          684 c          844 g         1134 t
ORIGIN      
        1 ccttgccgat acatggatgg tcggaaaata tgtgggcgac aatgcccttg cggcggtcgc
       61 aaggctaccc ctacgagttt atggttcttg catattgttg gctcaattat ccgagaaaat
      121 tacaaataat tacaaatggt tacaatttac aaaaatacaa ttgagcaggg ctgattatta
      181 tacacatttt ccacatagtt ttcaacaatt gttgaaaaag aacggcaatg cctgttgagc
      241 gttgctacgg ttactgcgga aaagggtaaa atgaaaaata attattgaat aacagcacga
      301 taatatgtta tactttggta aacaacaatc tttacggagg acaaaatgaa gaaaattata
      361 aataaaaagt cactcaaggg tacgcttgct gttatgttgt gtgccattat ggcgttttct
      421 gtttgtgcct gtgactttga ctctgacaca gaaccaacca aaacggatgc acaaattcag
      481 tttgataaat attgtgacga tttgttttcg gaagaacttg aggatgacgc tcttaccgca
      541 cattttgaca tttcaaatcc gtcggactac ggtctgaaat atgacgagga ggactatacc
      601 ctcggtcatg tgtccgatga agatacaaaa gaatcctttg atgaactgaa aaaggcaaaa
      661 acggatttgg aggagtttga ccgttcggga cttacttctt ctcaaaagca gacatatgac
      721 acccttgaaa gctactttga aattcagctt tcatatgagg gtacaacaga attgcagagt
      781 atatttgcac cgcagtcggg aattgttgca aacctcttta ccaccctgtc agaattcact
      841 ttctatgaaa aggatgatac cgacctttac cttgctgttt tgaaggacac aaaacgctat
      901 atggacgagt gcattgagtt cacaaggaaa caggctgagg acggttactt tatggctgaa
      961 gatattgcac agcagtcaat tgacgagtgc gaaaagcata ttaagaatga caagtccgtg
     1021 cttgttgacg agtttgagtc gagaataaag tcactcggtc ttagcgattc cgagaaaaag
     1081 actgttgtcg aaaccaataa aaagtatttt gaagaatact acattcctgc tttgaaatca
     1141 gcaaattcag cacttgaaag tctcaaaaaa tcaggcaaaa acgaagaagg tctttgcggt
     1201 tacggcaaaa tcggcaaaaa gtattattcg gcaattgtca aagacaaaac aagcagcagt
     1261 atgactcccg aggagcttaa gtcatacctt acaaacagct ttacaaaggt tggaatgagc
     1321 atgagcaatg tttcgcagga tgacttgagc aaatttcagg actacaaacc cgactttaag
     1381 gacgccgatg aggtgcttga atttttgatt gagaatatcg aggaggactt tccgactccc
     1441 gttacaacat cttacacggc agattatatg agcgattccg caaagagcga caatgtggga
     1501 gcgtactatg ttcagggacg aattgacgat acaagcgtta atatcatcaa aatcaacccc
     1561 gactttgcaa ataagggtat gactcagatg tacacaactc ttgcccatga aggctatcca
     1621 ggtcatcttt atcagtttac cgcatcaaat gcaaacaagg atatcccgaa tgtccgcaag
     1681 attttaagct ttatcggcgc aaccgagggt tgggcgcagt atgcctcgaa atgtacgctt
     1741 gattaccttg atacaagcga gggcattaag aagcttatct acgcaaatga tattctcggc
     1801 tatattctct actccatggt cgatgtcggt gtgaactaca acggctggga ttatgaaaaa
     1861 gtcaaggagt atatgtcaac ggcactcggt tcggctgaca gcgagagcgt tacagccgca
     1921 acaaaagagg cgtatgacct tgcccgttca aatccgggtt attttctccc ctatactgta
     1981 ggatacctca agatgattga tatgcgtgaa acagccgaaa gcgagcttgg ctcaagcttt
     2041 gacgcaaagg agtatcacac attcattatg aatctcggaa tcacaagcat tgatgtctac
     2101 gaaaaagccc ttgatatgtg gcttgaaagc aaaaagtaaa tttttggtga taatcttgaa
     2161 tattttttac aggtatgata taattaactc attgatatga taaatataaa ttggtatgga
     2221 tttttctaag atgtgattct tattttcgcc atacactcat tatgggtgta tggcgatttt
     2281 tattttacgc aaggggtgtt aatatgggcg ttaaaacgga aaacaaaatg gcttatgaac
     2341 ctgtcaacaa acttataatt caaatgtcgg caccgccgct gatttcaatg ttttttcagt
     2401 attcatacaa ccttgttgac agtatgtttg ttgcggggat aaatgaacag gcacttgcgg
     2461 cagtatcgct tgcattcccg attacaacgc ttatgaatgc gttgtccgtg tggataggtg
     2521 tgggtgttaa tgttttgatt gcaggttatt tgggacagaa aaatcagcaa aaggcaaatt
     2581 ctgcagcggc tctcggagtg gtaatttcgg tagttgtcgg agtgctgata aatttccttt
     2641 cgcttttcat aataaatccc tattattcgg cttttacgca aaacaaagag atttttgaat
     2701 acggaattgc gtatatgagc gtctgcgttt ttatgcagat accaaatatg gtgcatattg
     2761 taattcaaaa aatacttcag gcaacaggca atatgattgc tccgatgtgg tttcaaattg
     2821 caggtgttgt attcaatttt gtgtttgacc ctttgctcat tttcggcata ggtccgtttc
     2881 cgaaaatggg tgtggtcgga gctgcagttg ccacggtttt aggctatgtt gtttcaacaa
     2941 tcattgcgtt gatacagctg tttcttacaa aacaaaaggt taagccggtg tttaaaaact
     3001 gccttgaacc gcagatgata aagctgatat tttcctacgg attgccctcg tttgttatga
     3061 atgcgttggg agcattcacc gtgaattttg caaatatgtt tttggtgcga tattcggaca
     3121 cggcggttgc atttttcgga gcatatttca aggttcagca gttgattttt atgacggtga
     3181 acggactaat tcaaggctgt ttgcctataa tgcggtttaa ttacagagca aaagagtacg
     3241 gtcgccttaa acaagcaaat aagagcggaa caattatcgc gactgttatg atgattgtcg
     3301 gtatgctctt ggtgctgatt ttcccgaaag agatattgtt tttgttttcg gcatcggaag
     3361 aaatgtgtgc ttttggagtg ccggcaatga gaattatgtc actcggattt gtgttcagcg
     3421 gcttttctgc aatgattgca acctatgaac aggcaacaga cagtattatg cccggcgttg
     3481 tagttcaact gttgcgacag ggcgtgctgt tagtccccgt aatttggctt ttaaacagcg
     3541 ctgttcaaat gacaggcata tggcttgcgt tcccaattac agaatttgtc acatttcttg
     3601 ttgctgttgt atttgcaaaa attaaaaatc attgaataaa taaaaataac aaccgccaaa
     3661 acccgatata atggattttg gcggttgttt gttatttaaa atatgtttaa ttttaaattg
     3721 tttttgcaaa aacattatca gatgattttg ctctgaaaat tgtttttacg agcaagaatg
     3781 ttatataaac ataaagtgcg gtgtagat
//
LOCUS       DAOMVE010000150         3758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7113_length_3758_cov_1.800972, whole genome shotgun sequence.
ACCESSION   DAOMVE010000150 DAOMVE010000000
VERSION     DAOMVE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7113_length_3758_cov_1.800972"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PLS64_07865"
     CDS             complement(<1..439)
                     /locus_tag="PLS64_07865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HRL43775.1"
                     /translation="MSNVEITVFTPSYNRAKTLPRVFECLKKQTYKSFEWIIIDDGSE
                     DNTKDVVDGFLKENNFFDIIYVYQKNQGKHIATNNAVKMCRGNFFITLDSDDTCTNDA
                     LETFVNEWNKIPKQEQKKYYGISCRTYDKDGKINGIQMNVPYID"
     gene            complement(453..1982)
                     /locus_tag="PLS64_07870"
     CDS             complement(453..1982)
                     /locus_tag="PLS64_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRL43776.1"
                     /translation="MPKSAGKQMSINIIASIVSFAVTVGINFFLTPYLVKEVGSDAYG
                     FIGLANNFVQYATIVTTALNSISGRFISIAYHKGDVEKSSKIFSSVLVADLFLAAVML
                     ILSSIFVCFLDTVLKIPSNLVSGVKITFAFAFLTFVVSTVTAIFTTAAFVKNRIDINS
                     IRDIISNLIKVALIVLLFAIFPAQLYFVTLASFGSGIFLLLANITVKKKVLPEVKADI
                     RKFDIKIVKTLLASGIWLSLAQLCQVLLSGLDLLICNLTLGTALMGLLSIAKTVPNSI
                     GNLTVILGSVFTPHYTILYAKKKIPELIIEAKFSTKIVSFILSTPIAGFIAFGRQFYI
                     LWQPTKSPEEITMIQFMSVLTCITFLCSSQTQSLMMINTVCNKLRLPVFVNLTVGIVS
                     VIVAIAACNLTDYGVYFVAGTSSVLMGLRALIFTPAYSAYILKQKLSIFFPTVIRDCV
                     GFIIILVLFSIISNLITVTGWSSFIFICAICGILAYALSLPLLFNKAELCKLKNKLTK
                     K"
     gene            complement(1982..>2473)
                     /locus_tag="PLS64_07875"
     CDS             complement(1982..>2473)
                     /locus_tag="PLS64_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRL43777.1"
                     /translation="PVPKEPYIFLFSVRKPIKLISYALELSKKTGYKIYYLQKERLHK
                     IDGIEYLDPVNATEFVGLIKNAEYICTNSFHGTAFSVIYKKNFAVELQHIGGRNIRAE
                     ELLNNLGINDREITNDVFPNINGAVNWEKVDNVLLKERNKSKEYINKIKELIQNESSE
                     VTV"
     assembly_gap    2474..2483
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2484..3097)
                     /locus_tag="PLS64_07880"
     CDS             complement(<2484..3097)
                     /locus_tag="PLS64_07880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRL43778.1"
                     /translation="MKKIGIVTFHQALNYGAKLQAYALQQFLEKNGIENDVIDYTCHF
                     MYTRNIRPIRVGKHHKLKSFIRSLITMRQVGIDRKKSVTFRNEFLNLSRPFDAKNIGE
                     ASNEYAAFIAGSDQVWSPICVGFDTVYLLDFAKPKQKYSYAASFAVDKLPADKEHTYK
                     ELLSQFQLFSVREKSGADIIENLINRKAEISIDPSLLLTEDDWD"
     gene            complement(3094..3447)
                     /locus_tag="PLS64_07885"
     CDS             complement(3094..3447)
                     /locus_tag="PLS64_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43779.1"
                     /translation="MRVGGDRWNFNGHNCYFQIAVELGIVGSIFFLLFAVLSVVLTIL
                     AIRRVKNVCDDVRICYFSLYIQIMILAYSVTGNPTYSRQIVFIWFFSIGAVLHIARKH
                     NIDIVINKESERRHL"
     gene            complement(3455..>3758)
                     /locus_tag="PLS64_07890"
     CDS             complement(3455..>3758)
                     /locus_tag="PLS64_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43780.1"
                     /translation="IDILMLFVFIVSLMLTGKRTLFIIPVLSFALFMVISNIKGKFAK
                     TGGIVLSALSAVFILSMFIPKVANIFDRFMDEENIMALGNRDSLWRRIRLIQSVRI"
BASE COUNT         1199 a          683 c          673 g         1193 t
ORIGIN      
        1 tgtcaatata cggaacattc atttgtattc cgttaatttt accatcctta tcatatgtac
       61 ggcaggatat accgtaatat tttttttgct cctgtttagg aattttattc cattcattta
      121 caaatgtttc aagggcgtca tttgtacagg tatcatcaga gtcaagcgta atgaaaaagt
      181 ttcctctgca catttttaca gcattgtttg tggctatatg cttgccttga tttttttgat
      241 aaacataaat aatgtcaaaa aaattatttt ctttcagaaa tccgtcgaca acatcctttg
      301 tattatcttc actgccgtca tctataatta tccattcaaa gcttttatag gtttgttttt
      361 tcaaacattc aaaaactctt ggaagagttt ttgcacgatt ataggatggg gtaaatacag
      421 ttatttcaac attactcata attaactcca tttcattttt ttgtaagctt atttttgagc
      481 ttgcaaagtt ccgctttatt aaataacaac ggcaaggaca aggcatatgc aaggattccg
      541 catatagcac atatgaatat aaatgaactc caaccggtta ctgtaattaa attgctgata
      601 attgagaata ataccaatat aatgataaat ccaacacaat ctcggattac tgtcgggaaa
      661 aatattgaaa gtttttgctt tagtatgtat gcagaatatg caggggtaaa gatgagtgca
      721 cgcagtccca taagaacgga actcgttcct gctacaaaat atacgccata gtcggtgagg
      781 ttacaggctg caattgcaac tataacgctg acaatgccaa ctgtaaggtt tacaaataca
      841 ggtaatctaa gtttattaca tacggtgttt atcatcataa gcgactgagt ttgactggaa
      901 caaagaaatg taatacaagt caacaccgac ataaactgaa tcattgtaat ctcctccggt
      961 gactttgtcg gttgccataa tatgtagaac tgtctgccaa aggcaataaa accggcaata
     1021 ggagtcgaga gtataaagct tacaattttg gtactgaatt tcgcctcgat aattagctcc
     1081 ggtatttttt tctttgcata aagaattgta taatgaggag taaatactga acctaaaatt
     1141 acagttaggt tgccgatact gttcggtacg gtttttgcga tagagagaag tcccatcagt
     1201 gccgttccaa gggttaggtt gcatatcaga agatcaagac cgcttagaag cacctgacaa
     1261 agttgtgcaa gtgaaagcca aatgccggac gcaagaagtg ttttaactat tttaatgtca
     1321 aattttctaa tatcagcctt tacttccggg agtaccttct ttttaacagt aatatttgcc
     1381 agcaaaagga aaataccgct tccaaaactt gcaagggtaa caaagtaaag ctgtgcaggg
     1441 aaaattgcaa ataaaagtac tatgagtgca acttttataa ggttggatat aatatctcgt
     1501 atagaattta tatcaattct gttttttaca aaggctgctg ttgtgaaaat agcagtaact
     1561 gtgctgacaa caaatgtgag aaaagcaaaa gcaaaggtta tttttacgcc ggatacaaga
     1621 tttgaaggta ttttaagcac agtgtcaaga aaacaaacaa aaattgacga cagaatcaac
     1681 attactgcag cgaggaacaa atcggcaacg agaaccgaac tgaatatttt tgatgatttc
     1741 tcaacatcgc ccttatggta tgcaattgaa ataaatctgc ctgaaattga attcaaagcg
     1801 gtcgtaacga tggtagcata ctgtacaaag ttattagcaa gaccgataaa accataggca
     1861 tcactgccga cctcttttac aagatacggt gtcagaaaaa agttaattcc aacagtaact
     1921 gcaaatgaaa cgatacttgc aattatgttt atcgacattt gttttccggc ggattttggc
     1981 attaaacggt cacctctgaa gattcgttct gtatcagttc ctttatttta tttatatatt
     2041 cctttgattt atttctttcc tttaaaagta cattgtcaac cttttcccaa ttaaccgcac
     2101 cgtttatatt tggaaaaaca tcatttgtta tctcgcgatc attgattcca agattgttaa
     2161 gaagttcttc ggcacggatg tttctgccac cgatatgttg caattcaact gcaaaattct
     2221 ttttatagat taccgaaaag gcagtgccgt gaaatgaatt tgtgcaaata tattctgcgt
     2281 tttttattaa acctacaaat tcagtggcat tcaccggatc gaggtattct ataccgtcaa
     2341 tcttatggag cctctccttt tgcaggtagt agattttgta tccggttttt ttagaaagtt
     2401 caagagcgta gctgataagt ttaattggct ttcttacgct gaacagaaaa atatacggtt
     2461 cttttggtac aggnnnnnnn nnnttatccc aatcatcttc agtaagcaac aacgagggat
     2521 caatactgat ttcagctttt ctgttgataa gattttcaat aatatctgct ccgctttttt
     2581 cacgaactga aaagagctga aattgtgata acaactcttt atatgtatgt tccttatcag
     2641 caggaagctt atcaactgca aagcttgccg cataggagta tttttgcttt ggttttgcaa
     2701 aatcaagtaa atataccgta tcaaagccaa cgcagatggg actccacacc tgatcgctcc
     2761 ctgcaataaa tgctgcatat tcattggagg cttccccaat gtttttggca tcaaaaggac
     2821 gactgaggtt taaaaattcg tttctgaatg taacggactt ttttctgtca attcctactt
     2881 gacgcatagt gatgagtgaa cggataaatg acttcaactt atgatgcttg cctacgcgaa
     2941 taggtcttat gtttcttgta tacataaaat gacatgtgta gtcaattaca tcattttcta
     3001 tgccgttttt ttccaaaaac tgctgaaggg cataggcttg aagttttgct ccgtagttaa
     3061 gtgcctgatg aaaagttaca atacctatct ttttcataaa tgtcgccttt cagattcttt
     3121 gtttatcact atgtcaatat tgtgtttcct tgcaatatga agaacggctc cgattgagaa
     3181 aaaccagata aacacaatct gtcttgaata tgtcggatta cccgttacag agtaagctaa
     3241 aatcataatt tgtatatata gcgagaaata gcagattctt acatcatcgc agacattttt
     3301 tacccttctg attgcgagaa ttgtgagtac aacggaaagc actgcaaaca gaagaaaaaa
     3361 tatacttccg actattccga gctctaccgc aatctgaaag tagcaattgt gaccgttgaa
     3421 gttccatctg tctccgccta cacgcaatcc gttatcatat gcgaactgat tgtatgagcc
     3481 gaatcctgcg ccaaagagaa tctctattac ctaaggccat aatattttct tcatccataa
     3541 aacggtcaaa tatatttgca acctttggaa taaacattga aagaataaac acggcagata
     3601 aggcacttag cactattcct cctgttttag caaattttcc tttgatgttt gagatgacca
     3661 taaataatgc aaatgaaagg acgggaataa tgaaaagggt tcgttttccg gtcagcataa
     3721 gcgacacaat aaatacgaac aacataagaa tatcaatt
//
LOCUS       DAOMVE010000151         3751 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7130_length_3751_cov_3.754600, whole genome shotgun sequence.
ACCESSION   DAOMVE010000151 DAOMVE010000000
VERSION     DAOMVE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7130_length_3751_cov_3.754600"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..1442)
                     /locus_tag="PLS64_07895"
     CDS             complement(108..1442)
                     /locus_tag="PLS64_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HRL43781.1"
                     /translation="MNREKLGSRLGFIMLSAGCAIGCGNVWKFPWMCGQYGGGSFMLV
                     YIICLIVLGIPCLIMEFSVGRAAQTSPIKMYRKLEKPKQKWGGFGWACLVGNLAIMAF
                     YTVVCGWIIYYFINFLTGNNRDLGFTTMIANPSVNVIFMMITVVIAFVILSFNIQGGL
                     ERITKYMMIALLALMIVLAIHSLFLSGSGEGMTFYLKPDFSKINGDVVVGAMNQAFFS
                     LSTGMGGMAIFGSYIGKDHSLMGEAINVISLDTLVALLAGIIIFPACFTYNLEVNSGP
                     NLLFDTMATVFNNMPGGRIWGSLFFLFMVFAAMSTVLGVCENILAMIRDLTGWSRIKG
                     SLICGIVVFVLALTTALGFSVLHFQPFAEGTTWLDFWDFIVSTNVLPLGSLVLALFCC
                     NKFGWGWDNFIKEANMGIGMKVRSWMKPIFKYVVPTAIAFIYVYGLVTFKWR"
     gene            1621..2508
                     /locus_tag="PLS64_07900"
     CDS             1621..2508
                     /locus_tag="PLS64_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRL43782.1"
                     /translation="MDTNVLKTFVAVCEYSGFSAAAEKLGYTQSTVSSQIKQLESELN
                     TVLFDRFYHRISLTSDGITVLQHAREILESHEKMLEDLRTPETIEGNIRLAMSSSVCN
                     RFFKDDFLEFRKHFPNIHLVVVESGTEQMFDMLRKNEVDLIFTLDSHIYDSEFIICAE
                     HEEKVHFFASVDNPLLNKENISLKELCTEDFVLTESNMSYRKLLNNELAAQSLEIHPV
                     LEIGNPLQICSLIKNSKMISFLPDFITEDYYNPGEIKHLPVNDCDVSVWTQLLIHKNK
                     WKSPALNAFIDFYRDVIRK"
     gene            complement(2530..2625)
                     /locus_tag="PLS64_07905"
                     /pseudo
     CDS             complement(2530..2625)
                     /locus_tag="PLS64_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
     gene            complement(2625..2879)
                     /locus_tag="PLS64_07910"
     CDS             complement(2625..2879)
                     /locus_tag="PLS64_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HRL43783.1"
                     /translation="MTEREILLKKISTYEFAALDLQIYLDTHPHDTAMIQKMNGFKEK
                     LKPLVEEYEEKFGPLKKSMTSTNMWSWIKGPWPWENEEDC"
     gene            complement(2872..3072)
                     /locus_tag="PLS64_07915"
     CDS             complement(2872..3072)
                     /locus_tag="PLS64_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006568792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HRL43784.1"
                     /translation="MDIKEIISQQYGIPCLPKRTSEAMAYVPFQQFDSPTYSPLQGLE
                     SGTMFPVLNKPFYGKKCGEGLD"
     gene            complement(3227..>3751)
                     /locus_tag="PLS64_07920"
     CDS             complement(3227..>3751)
                     /locus_tag="PLS64_07920"
                     /inference="COORDINATES: protein motif:HMM:NF021062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HRL43785.1"
                     /translation="LAGFLRNKVSNPSAAAVTGTVGVCALRYICHVISGCTVWAGVSI
                     PSTDGLLYSLSYNATYMIPETIINAAAVFWLFGCLNFRSEKISVAKKIEKNLTETVTA
                     SISILSLMVAVIIDAVAVFASLQNPDSGVLDFSLISNTNFTLVGIVSAIGIVLCVVFA
                     IIAKVTSNSAKKVN"
BASE COUNT         1159 a          805 c          728 g         1059 t
ORIGIN      
        1 cgtaggattc aaaacctaca gagaatacaa ggaaatgaat atttaaaccg aaaaaatccg
       61 tatgaagatt gctccatacg gattttattt tgtgtatcaa attttattta tcgccattta
      121 aaggtgacga gtccgtaaac atagataaat gcgatagctg taggaactac atatttgaaa
      181 atcggtttca tccacgaacg cactttcata ccgatgccca tgttggcttc tttaatgaag
      241 ttatcccaac cccagccgaa cttgttgcaa cagaacagag caagcacaag agaaccgaga
      301 ggaagaacat ttgtggaaac aatgaagtcc caaaaatcaa gccatgttgt accctctgca
      361 aacggctgaa aatgaagaac gctgaatccg agagccgttg tgagtgcaag cacaaacact
      421 acaattccgc aaataagact gccttttatt cttgaccagc ctgtcaaatc acgaatcatt
      481 gcaagaatat tttcgcaaac acccaaaacg gttgacattg ccgcaaacac cataaacagg
      541 aagaagagcg aaccccatat tcttccgccg ggcatattgt tgaacacggt tgccattgtg
      601 tcaaacaaaa ggttcggacc tgaattaact tcaaggttgt aagtaaagca ggcagggaag
      661 atgattatgc ctgcaagcag agctacgagt gtgtcaagag agataacatt gattgcctcg
      721 cccataagtg aatggtcttt gccgatataa cttccgaaaa tagccatacc gcccatacct
      781 gttgacagcg agaaaaatgc ctgattcatc gcacccacaa caacatcacc gttgattttt
      841 gaaaagtcgg gtttgaggta aaatgtcata ccctcacccg aaccgctcaa aaacagactg
      901 tggattgcaa gcacaatcat aagtgcaagc aaagcaatca tcatatattt tgttatcctc
      961 tcaagtccgc cctgaatgtt gaacgagagt ataacaaagg cgataacaac cgttatcatc
     1021 ataaaaataa cattgactga cggatttgca atcattgtgg taaatccgag gtcacggttg
     1081 ttacccgtca gaaaatttat gaagtagtaa attatccaac cgcaaacaac tgtgtaaaaa
     1141 gccataattg caaggttgcc cacaaggcat gcccaaccga aaccgcccca tttttgcttt
     1201 ggcttttcaa gtttgcggta cattttaatc ggacttgtct gtgccgctct gcccaccgaa
     1261 aattccatta tcaaacacgg aattcccaaa acaataagac agatgatgta cacaagcata
     1321 aaacttccgc caccgtattg tccgcacatc cacggaaact tccacacatt tccgcaaccg
     1381 attgcacagc ctgccgaaag cattataaag ccgagtcggc ttcctaattt ttcacgattc
     1441 acaacacaag tcctcctgcc gaatttattt tatctacaca atattttttc gtcataaata
     1501 ctgctccttt gtttattttg caatacaaag agtagtatag caaataaaat accatttgta
     1561 aaattgataa aactgtgcta taataacgaa gatatcgttc gtataaaaga ggatttagtt
     1621 atggatacca atgtgcttaa aacctttgta gctgtgtgcg aatattcggg attttctgct
     1681 gcagcggaaa agctcggcta tacccagtca accgtttcat cacagataaa acagttggaa
     1741 agcgagctta acacggtgct ttttgaccgc ttttaccaca gaatcagcct gacttccgac
     1801 ggcataacgg ttctgcaaca cgcaagagaa attcttgaat ctcacgaaaa gatgcttgaa
     1861 gatttgcgta cccccgaaac aattgagggc aacattcgcc ttgcaatgtc aagttctgtc
     1921 tgcaaccgat tttttaaaga tgattttctt gagttcagaa agcattttcc taatattcac
     1981 cttgtcgttg tcgaaagcgg tacagagcag atgtttgata tgttacgcaa aaatgaggtt
     2041 gatttgattt ttacgcttga cagccacatt tatgattcgg aatttataat ttgtgcagag
     2101 catgaggaaa aggttcactt ctttgcatca gttgacaatc cacttttgaa taaagaaaat
     2161 atttcgctaa aagaattatg caccgaggat tttgtgctga ccgaaagtaa tatgagctac
     2221 agaaaattgc tcaacaatga acttgccgca caatcgcttg aaattcaccc tgttttggaa
     2281 ataggaaacc ctctgcaaat ttgttctcta atcaaaaaca gcaaaatgat ctcgttcttg
     2341 cctgatttca tcacggagga ttactacaat ccgggagaga taaaacatct tcccgtcaac
     2401 gattgtgatg tttcggtgtg gacgcagttg cttatccaca aaaataagtg gaaatctcct
     2461 gcgcttaatg cgtttattga tttttatcgg gatgttatac gaaaataaaa agtatcacat
     2521 tttgccctac gcaccgccgt attgcgttat gataatcttg gcaagctttg cgttcggctt
     2581 tttgatattg acggggtact gtaacttttt ttcatagaca aacatcagca atcctcctcg
     2641 ttttcccacg gccacggtcc ctttatccag ctccacatat tggtgcttgt catactcttt
     2701 ttcaaaggac cgaatttttc ttcatattct tcaacaagcg gtttcaattt ttctttgaaa
     2761 ccgttcattt tttgtatcat tgccgtatcg tgcggatgag tgtcaagata aatctgaagg
     2821 tcaagtgcgg caaattcgta ggttgaaatt ttctttaaaa gaatttctct ttcagtcaag
     2881 tccctcaccg catttctttc cgtagaatgg tttgttgaga acggggaaca ttgtacccga
     2941 ctcaagcccc tgcaacgggc tgtaggttgg ggagtcgaac tgctgaaagg gaacatatgc
     3001 catagcctcg ctcgttctct ttggcagaca cggtattccg tactgctgag aaataatttc
     3061 tttaatatcc aatttaattc tcctttttgt tcaaacttat gaacattgct gttcattctc
     3121 ataatatgcc cgtttcatac ttgtgtttac ataataaaaa aacagagccg acagcaatgc
     3181 cgtcagccct gatacagagg ttttaccgtg aatatccgat taaatgttaa ttaacctttt
     3241 tggcagaatt tgaagtaacc tttgcaatga ttgcaaaaac tacgcaaagc acaattccga
     3301 ttgccgaaac gatgcctaca agagtgaagt ttgtgtttga aatgagtgaa aaatcaagca
     3361 caccgctgtc ggggttctga agtgacgcaa aaactgcaac agcgtcaata attacggcaa
     3421 ccataagtga aagaattgaa attgatgccg taacagtttc tgtaaggttc ttttcaattt
     3481 tctttgcaac gctgattttt tcacttctga agttaagaca gccgaaaagc cagaatacag
     3541 ccgccgcatt gattattgtt tcgggaatca tatatgtagc gttgtagctg agtgaataga
     3601 gaagtccgtc ggttgagggg attgaaacac ctgcccatac ggtacagcct gaaattacat
     3661 gacagatata tctcaaagca caaactccta ccgtgcctgt tacggcagcg gcagacggat
     3721 tgctgacctt gtttctgaga aagcctgcaa g
//
LOCUS       DAOMVE010000152         3728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7193_length_3728_cov_2.528995, whole genome shotgun sequence.
ACCESSION   DAOMVE010000152 DAOMVE010000000
VERSION     DAOMVE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7193_length_3728_cov_2.528995"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLS64_07925"
     CDS             complement(<1..345)
                     /locus_tag="PLS64_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRL43786.1"
                     /translation="MTRVLIVEDQSMPRTLFEMYVNQSENYTLAGSISNADMTDYHCE
                     NSDVDLILMDICTSMGANGLEAAERIKAKFPKIKIIIVTSMPEYSYLKRAKDAGVDSF
                     WYKEVSSEPIIEL"
     gene            complement(323..1405)
                     /locus_tag="PLS64_07930"
     CDS             complement(323..1405)
                     /locus_tag="PLS64_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43787.1"
                     /translation="MAEFFGRMPFALSVGITAYCAIMSVYITVRLHHCRNTDITAMSI
                     KESFDGLPTGLCYFDENGLVVLKNNLMESICLELTGKSLNVAMPFREKIKEASQYEND
                     GAFTVRLKDGRVFSFSLSETEIFSKPYFQLAATDITEFTKLTSTLEYEIGELKKVNAH
                     LKKYNEMVYDLTREEEILRTKEIIHDRLGSTLITTRRYLENGTLSENNSKLLEMWKNN
                     ISVLNTAENDCKDENFAKDIENAAKLVGITLETVGNLPTDNNAVARVLTFATMQCLNN
                     AARHAKADKMILESLDDGEKYIFKFANNGRKPPRKINEGGGLSSIRKKAEQIGGSMKI
                     STSEIFVLEITIPKSEVLQYDKSADS"
     gene            complement(1627..3294)
                     /locus_tag="PLS64_07935"
     CDS             complement(1627..3294)
                     /locus_tag="PLS64_07935"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRL43788.1"
                     /translation="MVRKVDKTKLLTVVGIIIFLFGGAVRIFSHLTSSADNYIENFDV
                     IIFSGLIIGWGVSVAYRIVQKNIRICLVISAALMLLWMALRAIKYNSPADINTYGRYL
                     WYSYYIAMVFLPLMMFFAMLNIGKPENTNNRKYLLIIPAAVLVLLVMTNDFHQLAFVF
                     EPDFHNWNKQYSYGPVYYVIAVWIFILVLSSIVLSINRCRISATRKKLWIPIVIILVA
                     IIYTLWNNLNHGYSGLRIYNVPEVFCFASIALWESLIQIGLVPSNTGYGDFFNASNLN
                     TLIFDNEGNVKYRSKNATNVSKDVVLQNGNSVVIDENIILKKHNIKGGKAVWTEDISA
                     INRINRELSEVKEQISEYNVILKSEAELKKRRAAVTEQNKLYDSITEFIRPQLCDLEN
                     ILKNIENNRGDISVNYAGACVVNVYIKRISNLLIMAKSRKMLNAFELENSIRELAEYI
                     SVYGISCSFFSNVSGEISAEKTIALFKFIGIFIRHIMNCTDALLFNLKVNEKFIDLKI
                     NCDSKNEMKIPDDLLDDIIKLGGNVTTEYDADTLFVFVRMQSGGDDI"
BASE COUNT         1128 a          771 c          621 g         1208 t
ORIGIN      
        1 aagttcaata ataggttcgg aacttacctc tttgtaccaa aagctgtcaa ctcctgcatc
       61 cttggcacgc tttagatagg aatattcggg cattgaggta acaatgatta tcttaatttt
      121 cgggaacttt gcttttattc tttcagccgc ctcaagaccg tttgctccca ttgatgtgca
      181 tatatccatt aaaatcaggt caacatcgga attttcacaa tgataatccg tcatatcggc
      241 attacttatt gagcccgcaa gcgtatagtt ttcgctttgg tttacataca tttcaaataa
      301 agttctcggc attgactgat cttcaactat cagcactctt gtcatactgc agcacctcgc
      361 ttttcggtat tgtgatttca agaacaaaaa tttcggaagt cgatattttc atgcttccgc
      421 cgatttgctc cgcctttttt cttatggaag aaagtccgcc gccctcattg atttttcgag
      481 gcggttttct gccgttattt gcaaatttaa aaatatattt ttcaccgtcg tcaagacttt
      541 caagaatcat tttatctgct tttgcgtgtc ttgcggcatt gttaaggcat tgcattgttg
      601 caaaagtcag aacccgtgca accgcattgt tatcagtcgg aagatttccg actgtttcaa
      661 gcgttattcc gacaagcttt gcggcatttt caatatcctt agcaaagttt tcgtctttgc
      721 aatcattctc tgcggtattc agaacagaaa tattgttttt ccacatttca agtaatttac
      781 tgttattttc agacagagtg ccgttttcaa gatatcttct tgttgttatc agcgtactgc
      841 cgagcctgtc atgaattatc tcttttgtgc gaagaatttc ttcttctctt gtcagatcat
      901 agaccatttc attgtatttt ttcagatgag cattaacctt ttttaactcg cctatttcat
      961 attccaaggt tgatgtaagc tttgtaaact ctgttatatc ggttgccgca agctgaaaat
     1021 acggttttga aaaaatttcc gtttccgaca acgaaaagct gaaaaccctg ccgtccttta
     1081 atctgactgt aaatgctccg tcattttcgt attgagaagc ctcttttatc ttctctctga
     1141 agggcattgc aacattaagc gatttacccg taagttcaag gcatatgctt tccataaggt
     1201 tatttttcaa tacaacaagt ccgttttcgt caaaatagca aagtcctgtc ggcaagccgt
     1261 caaagctttc tttaattgac attgcggtaa tatcggtatt cctgcaatga tgaagcctta
     1321 cagttatgta cacagacatt atcgcacaat atgccgttat gccgacagac agagcaaacg
     1381 gcattctgcc aaaaaattca gccattttat taatttggtt atcgtaatat attttactca
     1441 agacatgagt gcttaccaaa aacattgtca aagagattac tgcaattata accgctgaaa
     1501 tcggtttcca caacctgatt ttataaagaa aagattgcgt tgcgttgtat attgccgcac
     1561 aggcaacaac aaattgcaca ctcatcaata taacacaaac aaacgaacgt atttcaagga
     1621 tggatgtcat atgtcatcac ctccgctttg catacgaaca aacacaaaca gcgtgtcggc
     1681 gtcatattcc gtggtgacat ttccgccgag ttttataata tcgtcaagta aatcatcggg
     1741 aattttcatt tcatttttgc tgtcacagtt gatttttaaa tcaataaatt tctcattgac
     1801 ttttaaatta aacagcaacg catctgtgca gttcattata tgccttataa aaatcccgat
     1861 gaatttaaaa agtgcaatcg ttttttcggc tgaaatttct cccgaaacat tactgaaaaa
     1921 cgaacacgaa attccgtaaa cagaaatgta ctcggcaagt tctcggatac tgttttcaag
     1981 ctcaaaagca ttcagcattt ttctgctttt tgccataatg agcagattgc ttattctctt
     2041 gatgtagaca ttaacaacac aggcaccggc ataattaacc gaaatatctc ccctgttgtt
     2101 ttcaatattt ttgagtatat tttcaaggtc gcaaagctga ggacggataa attcggttat
     2161 actgtcatag agcttgttct gttcggttac tgccgctctt cgttttttca gctctgcctc
     2221 tgattttaag attacattgt attccgaaat ctgctctttt acttccgaaa gttctctgtt
     2281 aattcggtta attgccgata tatcttccgt ccaaactgcc ttaccgcctt ttatattatg
     2341 ctttttaaga attatatttt catcaatcac aacactgtta ccgttttgca gaacaacatc
     2401 ttttgaaaca tttgttgcgt tttttgagcg gtatttcaca ttgccctcat tgtcaaatat
     2461 gagagtgtta agatttgacg cattgaaaaa atcgccgtaa cccgtatttg acggcacaag
     2521 acctatctga ataaggcttt cccataaagc aattgaggca aaacagaaca cctcgggaac
     2581 attgtaaatt ctaagtcccg aatatccgtg attcagatta ttccagagtg tgtaaattat
     2641 tgcgacaaga attattacaa tcggaatcca cagctttttg cgtgttgcgg aaattctgca
     2701 tcgatttatt gaaagcacaa ttgacgaaag tacaagtata aaaatccaca ctgcaattac
     2761 atagtaaaca ggaccgtaac tgtattgttt gttccaattg tgaaaatcgg gttcaaatac
     2821 aaaagcaagc tgatgaaaat cgttcgtcat aacaagcagc acaagaacgg cggcaggtat
     2881 aatcaaaaga tattttcggt tatttgtatt ttcgggctta ccgatattca gcattgcaaa
     2941 aaacatcata agcggcaaaa acaccattgc tatgtaatat gaataccaaa gataccgtcc
     3001 gtatgtgttg atgtcggcag ggctgttgta ttttatggca cgaagagcca tccacaagag
     3061 cataagcgcc gcagatatca caaggcagat tcgtatattc ttctgaacaa tgcgatatgc
     3121 aacagacaca ccccagccta taataagccc cgaaaagata ataacatcaa aattttcaat
     3181 ataattgtcc gccgaggaag taagatgaga aaaaattctc accgcacctc cgaacaggaa
     3241 tataattatc ccgacaacag tcagaagctt tgttttgtca actttgcgaa ccatatgcca
     3301 cccgtcagtt atttcaagtt taatttcagc aacataattt tttcaaaaca ccaatgttgc
     3361 acaccatttt actacataat aacacaataa aacggattat acaattcata tttaccgcaa
     3421 acttaaacaa aaaagtaaat agttaattcc caaagtaact tccacggtga aacttacttt
     3481 ggaaattaac gcactgaaat cttccttgtt ttggactgta atcaatcgtg gtattcttaa
     3541 caaacttatt aaactacaaa aggcacataa aaaggcacat aaccgaaagt gataacggtt
     3601 atgtgccata ttgttttttg caatgctgtt ttttcacagt ccacgagtaa aatttgtacc
     3661 ctaattagat ttacactatt ctcaaactac aattatcgta actgcgaaga tatcttagtt
     3721 ttactacc
//
LOCUS       DAOMVE010000153         3637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7442_length_3637_cov_4.315187, whole genome shotgun sequence.
ACCESSION   DAOMVE010000153 DAOMVE010000000
VERSION     DAOMVE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7442_length_3637_cov_4.315187"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLS64_07940"
     CDS             <1..806
                     /locus_tag="PLS64_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HRL43789.1"
                     /translation="DFEVTGTTQKISNNQLFVTTTGYTENTAKNFGAMFISTDYQKYS
                     VMFKDCIDAPKFVNLPKNQEFTRHMITADNRYFGNTGMKFNYDSTGGEYVKIRIKLSD
                     YFPDVFNDDGTMTGKLSDGTYKNFDFTYEDFGTDSSGKPCYATSGAYFVSGGAVTSFV
                     PDKNGYAEFYISRQLGEDTGLMAYKYCRLKNGAECKTTIGGKLNCLTCGDAYPDGNIT
                     VGDATSVQKYVVGIVKFGKTQLFNSDVNHDGEISVVDATLIQKYIVGLS"
     gene            complement(1125..1766)
                     /locus_tag="PLS64_07945"
     CDS             complement(1125..1766)
                     /locus_tag="PLS64_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRL43790.1"
                     /translation="MTDMVKKYVPYAVIILLVYMLLPLVFIPGAMQKFSPVAYDFVFP
                     ITAIGCSAHYCSKHGLDFLFALIAPIIYLPSMLIYNGGFSSSTLSTNLILLVVYLVAG
                     IFGLFLGDLAFGDKRRQKEKAEQEEAEEMLLEAKRREKEELENMTSSSKKSERKAPYR
                     QERKPARRPSPAPQKTETADDDDFDYDKYLSGIDRKQDPNESEIDDILNEYGH"
     gene            2190..3557
                     /gene="mgtE"
                     /locus_tag="PLS64_07950"
     CDS             2190..3557
                     /gene="mgtE"
                     /locus_tag="PLS64_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005944661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HRL43791.1"
                     /translation="MEQVNVTLTETIKVLLDDKKFSTLRDILITMKPFDIAAVFENLQ
                     DEKMPILFRILPKELAAETFVEMDDETQEFLIHGFSDSELKEVVDELFVDDAVDLIEE
                     MPANVVKRILRQADKDMRKQINELLKYPEDSAGSIMTTEFIVLRPDMTAEMAIKRIRR
                     TGVDKETIYTCYVTDANNKLIGITTVKDLLLAEDDELVKSIMEENVISVTTLADQEQV
                     AQMFSNYNFLALPVVDNENRLVGIVTIDDAIDVIQEEATEDIEKMAAVLPSDKPYMKT
                     SVFGLYKKRAPWLLILMLSATFTSAIISSFEAVLANVLILSSFIPMITGSGGNAGSQA
                     SVSVIRALSLGEIHFRSIFLVLWKEFRVSILCGITLAAANFVKLLLFDLNGQENAFMI
                     ALVISLTLVGTIIMAKLVGSSLPLLASKVGFDPAVMANPLISTVCDSLSLLIYFGVAK
                     LILHL"
BASE COUNT         1065 a          694 c          806 g         1072 t
ORIGIN      
        1 aggactttga agttacaggt acaacgcaga aaatctcaaa caatcagctt tttgttacaa
       61 caacgggata taccgaaaat acggcaaaga atttcggggc aatgttcata tcaacggatt
      121 atcaaaagta cagcgtgatg tttaaggact gtattgatgc accgaagttt gtaaatctcc
      181 cgaaaaatca ggaatttact cgccatatga taacagcgga taatcgttat tttggtaata
      241 cgggtatgaa attcaattat gattcaacag gcggagaata tgttaagata aggattaaac
      301 tctccgacta ttttcctgat gtctttaatg atgatggcac aatgaccgga aaactcagcg
      361 atggcacata caagaatttt gactttacat atgaggattt cggtacagac agcagcggca
      421 aaccttgcta tgcaacctcg ggcgcatatt ttgtcagcgg aggtgcggta acatcttttg
      481 tccccgataa gaacggctat gcggagtttt atatttcaag acagcttggc gaggatacgg
      541 gccttatggc ttataaatat tgcagactta aaaacggcgc agagtgtaaa acaacgattg
      601 gtggcaaatt gaactgcctg acttgcggtg atgcatatcc agacggaaac attacggttg
      661 gagatgcaac atctgttcag aaatatgttg taggtattgt aaaattcggc aagactcagc
      721 tttttaacag cgatgtaaac catgacggtg aaatcagtgt tgttgacgca accttgattc
      781 agaagtatat cgttgggttg agttaaataa aaattacata taaaagcaaa ttgacgaagt
      841 aatggtacaa tctttttgat aagacagatt atatatgtgt cgcaaattac acacaacttc
      901 cataacatcg actgattttg cgaaaacata aaattcaatc aaatcgggcg tccgatgaac
      961 acccctgctg attaaacaaa aacagtcggg aagacaatgc tttgcatttc gcaaattgtc
     1021 ttcccgactt taattttaca tttttagtta tagagttctg ctagggtatt ttattttcgc
     1081 ccttataaat actatctgca cgaactggga acggcggctc gccgttagtg tccgtattcg
     1141 tttaagatat cgtcaatttc gctttcgttc ggatcttgct ttctgtcaat accggaaagg
     1201 tacttgtcat aatcaaaatc atcgtcatct gctgtttcgg ttttctgcgg tgcaggcgac
     1261 ggtctgcgtg caggcttgcg ttcctgtctg taaggtgctt ttctttcgga ctttttgctt
     1321 gatgaagtca tgttctcaag ctcttccttt tcacggcgct ttgcctcaag aagcatttcc
     1381 tctgcttctt cctgttctgc cttttctttc tgacggcgct tgtcaccgaa agcaaggtcg
     1441 ccgaggaaaa gtccgaaaat tccggcaaca agataaacta cgaggagaat aaggtttgtt
     1501 gaaagtgtgc ttgaagaaaa tccgccgttg tagatgagca tactcggaag ataaataatc
     1561 ggcgcaatga gtgcaaagag aaagtcaagt ccgtgttttg agcagtagtg tgccgaacag
     1621 ccgattgctg taatcgggaa cacaaagtca tatgcaacgg gtgaaaactt ctgcattgcg
     1681 cccggaataa acacaagcgg caacagcata taaacaagca aaattattac cgcataggga
     1741 acatattttt taaccatatc tgtcataacg gttaatctcc aatctttgaa attttatttt
     1801 aaatcatgca tattcattat acattgatac acttcaaaaa gcaagaaata ttataccgag
     1861 ttaccaagct gttacagagg ataatgtaaa atgtttgcaa agtggatttt tagcggataa
     1921 atccgttgac atcggacata aaaaagaata aaataaatat gtacgggctt ttgcccgaat
     1981 ttaaatgcga atgaggtgaa aacttaatgg acgcagacgg taataaaggc gctgtacccg
     2041 ggcgaagttg caacagaatg tgcgcttgtg cgttcatttt gttttattga ttttgctttg
     2101 tatttttatt ttaatttgaa ttgatttaat ttaatactca gggtgacacc gtctttactt
     2161 ccaattgaat taaaaaggag gaaaagatga tggaacaggt taacgtaacc cttaccgaaa
     2221 ctatcaaagt gttgcttgat gacaaaaagt tcagcacttt gcgtgacatt ttgattacga
     2281 tgaaaccgtt tgacattgcg gcggtttttg aaaatctaca ggatgaaaaa atgccgattc
     2341 tgtttcgtat tctgccaaag gagcttgcgg cggaaacctt tgtcgaaatg gatgacgaaa
     2401 ctcaggaatt ccttatccac ggcttcagcg acagcgaact gaaagaggtt gtcgatgagt
     2461 tatttgtcga tgacgccgtt gaccttattg aggaaatgcc tgccaatgtt gtaaagcgta
     2521 ttttaagaca ggccgacaag gatatgagaa agcagattaa cgagcttttg aaatatcccg
     2581 aggacagcgc aggctcgatt atgacaaccg agtttattgt gcttcgtccc gatatgacgg
     2641 ctgaaatggc tattaaaaga atcagaagaa caggtgttga caaggaaaca atctacacct
     2701 gctatgtaac cgatgccaac aacaagctta tcggtattac caccgtaaaa gaccttttgc
     2761 ttgccgaaga tgacgagctt gtaaagtcga ttatggagga aaatgttatc tcggtaacta
     2821 ctcttgccga tcaggagcag gttgcccaga tgttctcaaa ctataacttc cttgctcttc
     2881 ctgttgttga taacgaaaac cgacttgtcg gtatcgtaac aattgacgac gctattgatg
     2941 ttattcagga agaggctacc gaggatatcg aaaagatggc agccgtattg ccgtctgaca
     3001 aaccgtatat gaaaacaagc gttttcggtc tgtacaaaaa gagagcgccg tggctgctta
     3061 tactcatgct ttcggcaacc tttacaagtg cgattatctc atcatttgag gctgtgcttg
     3121 caaatgtttt gattctctca tcgtttatcc cgatgattac gggttcgggc ggtaacgcag
     3181 gttctcaggc gtcggtttcc gtaatacgtg cgttgtcgct cggtgaaatt catttcagga
     3241 gcattttcct tgtgctgtgg aaagaattca gggtgtcaat tctttgcggt attacccttg
     3301 cggcggcaaa cttcgtaaaa ctgcttttgt ttgatctcaa cggtcaggaa aacgcattta
     3361 tgattgccct tgtaatttca cttacgcttg tcggcacaat tattatggca aagcttgtcg
     3421 gcagcagtct gcctcttttg gcaagcaagg tcggctttga ccctgccgtt atggcgaatc
     3481 cgctcatttc aacagtctgc gactcgcttt cattgcttat ctatttcggc gttgcaaagc
     3541 tgattttgca tctgtgattt ttttaaaatt gaaagttgaa aatggaaaat tgaaaattgc
     3601 ggtcgggcag ccagtttgca aaatgcaaat cattgtt
//
LOCUS       DAOMVE010000154         3623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7480_length_3623_cov_2.517377, whole genome shotgun sequence.
ACCESSION   DAOMVE010000154 DAOMVE010000000
VERSION     DAOMVE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7480_length_3623_cov_2.517377"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..305)
                     /gene="ssrS"
                     /locus_tag="PLS64_07955"
     ncRNA           complement(114..305)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLS64_07955"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(360..656)
                     /locus_tag="PLS64_07960"
     CDS             complement(360..656)
                     /locus_tag="PLS64_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HRL43792.1"
                     /translation="MANEYTPDLITLIGDDGKEIEFEILDIIENDEGKFYVLYPFFEN
                     PEDAVNDSGEYYIFEVTEIDGEEELAEIEDDDKLDRIAAVFEERYNEEFYDEDD"
     assembly_gap    801..810
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1140..1820
                     /locus_tag="PLS64_07965"
     CDS             1140..1820
                     /locus_tag="PLS64_07965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRL43793.1"
                     /translation="MKSFQKLPPQFQCAEVKEYYDILCKKQGSFVLKRILDIFASVIL
                     LVFLIIPIAIIAILVKTDSKGPVFYRQERVTTYGKKFRILKFRTMVTGADRLGTLVTT
                     DSDSRVTKTGRILRKYRLDELPQIFNVLSGSMSIVGTRPEVQHYVDMYEPEYLATLLM
                     PAGITSLASIMYKDEEKLLKGEIDVDRVYVEKILPEKMKFNLSYVKNFSFGSDIKLMF
                     KTVKEVFS"
     gene            1845..3347
                     /locus_tag="PLS64_07970"
     CDS             1845..3347
                     /locus_tag="PLS64_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRL43794.1"
                     /translation="MTDFKQKLRGFFSDSSLFRRIYIIDLFFTNIAFLQIPAYVLLVF
                     LFIWGVCLSVYNQRHNNTFFKLRFGIWIGAFLAVTVLSMLINFSQTFLFSLLMLLHVV
                     MCFFLFYGMHTEPEFDYRIELYHIAKFIMYATTVMNIIGITCLMFGFKFEWYWIKFTV
                     YENRFTGCYVNPNLLGFISVVSIFCCHILSKGHFMRRIAEKIPEPGISKIWIVACLAT
                     NAFSLILCDSNASLVLALGYAIVYIVYMFFADKAGLSPSKIILKITALFLVGVFLTGS
                     ALMFRTICQEGFSVVVSKTTSVVDLLLGKTESELVQEELTPEQREQLEKDKITFSHEN
                     KNIDSGRKKLWLESINLFKLSPIIGISNGNIVLYSAEYSNGALEYSYHKSDLHNGFLT
                     ILVSTGVIGFVLFGIFGFRFAKHSAQHLFLQKKTYRDDVYPCLFAFLFAYLIFACFEK
                     ALLYDISFMVVWFWLIMGYMSCYITKFEPTLESQYLFHGKRLRRVTRTML"
     gene            3432..>3623
                     /locus_tag="PLS64_07975"
     CDS             3432..>3623
                     /locus_tag="PLS64_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRL43795.1"
                     /translation="MKYGFIKVASATPKIRVADCKTNTINIIEQIKEAHKNGASLVVF
                     PELCVTGYTCSDLFYQRVLL"
BASE COUNT         1061 a          674 c          674 g         1204 t
ORIGIN      
        1 gagataccag gcaatgcagc tgccccagcg tccgcaacct aaaacggaaa tattcatatt
       61 attactactc ctgtttatat attatttata ttttttatag ctttaataac aataaaaaac
      121 ccgcccgacc gtatgatttt tgcaataacc tgcctacttg agtgacaggt gggtgcccgc
      181 ccttgaactt caggctccct tcttccgaga aaacgggagg tctgcacacc gttcaatgga
      241 gctaagctcc cgatttaaat gttgtagggt tattttaaaa tcattcgcgt atcgggcagg
      301 aattaaaact tattaatctt tagtcctgca atactattta tatgcagaaa aatgaactgt
      361 tagtcatctt catcataaaa ttcttcattg taacgctctt caaaaacagc ggcaattctg
      421 tcgagcttgt catcatcctc aatttcggca agctcttctt cgccgtcaat ttcggtaact
      481 tcaaaaatat aatattctcc cgaatcattt acagcatcct ctggattttc aaagaacggg
      541 tagagtacat aaaacttacc ctcatcgttt tcaatgatat caagaatttc aaattctatt
      601 tctttaccgt catcaccgat aagggtaatg aggtcgggtg tatattcgtt agccattcca
      661 gaacctccgg tttttaagaa atttgggttt gctcttttca ccgtaatcat tttacaattt
      721 tttgagcgtt tagtcaagat gtttaacact tttgattaaa ataattaaaa agtattaaaa
      781 aaatggaaaa gttgaaaaaa nnnnnnnnnn tgttgacaat gactaaaagg tatggtatag
      841 tataaacacg ataaatcata tatcaatatt aattaactaa caacttcctt tctatgtgct
      901 tgattactcc ataaaattac ctaaaaaccg acggctgcat accgtcggtt tttacgttgt
      961 gttaaatttt tttattgtaa actttttgta agacaaatag ccttgaaaaa tgattttaaa
     1021 agatatataa tgcttttgat aatggattag tgtgggaaaa taaggaaaaa tcaactttta
     1081 tcgcataaat ccgttccgcc gtatgttcaa tatacacaca gaatggagtt ttttatttaa
     1141 tgaaatcatt tcaaaagctt ccgccgcagt ttcagtgtgc cgaagtaaaa gaatactatg
     1201 acattctctg caaaaaacag ggaagttttg ttttaaagcg aattcttgat atttttgctt
     1261 ctgttattct tcttgtgttt ctcatcattc cgattgcaat tattgcgatt ttagttaaaa
     1321 ccgactcaaa aggtcctgtg ttttacagac aggaaagggt tacaacctac ggaaagaagt
     1381 tcagaattct taaattccgt acaatggtaa cgggagccga caggctcgga acacttgtca
     1441 caaccgacag cgactcccga gttacaaaaa caggaagaat tttgcgaaaa tatcgtcttg
     1501 acgagctgcc gcagattttc aatgttcttt cgggcagtat gtcaatagtc ggaacccgtc
     1561 cggaggttca gcattatgta gatatgtacg aacccgaata tcttgcaaca ctccttatgc
     1621 ctgccggaat aacctcgttg gcttcaatta tgtataagga cgaagaaaag ctgttgaaag
     1681 gcgagataga tgttgacagg gtttatgttg aaaaaattct tcccgaaaaa atgaagttca
     1741 atctttcata tgtcaaaaat ttcagctttg gttccgatat aaagctgatg ttcaaaaccg
     1801 taaaagaagt tttcagctga tttcgttttg attggagtat tcagatgacc gattttaaac
     1861 aaaagctcag aggatttttt tccgattcat cgctttttag aagaatctac attatagatt
     1921 tgttttttac aaacattgcg tttctccaga ttcctgcgta tgtacttctt gtttttctct
     1981 ttatatgggg tgtatgcctt tcggtatata atcaaagaca taataatact ttttttaaac
     2041 tgcgcttcgg aatatggatc ggagcatttc ttgccgtaac ggtattgtcg atgctcatca
     2101 atttttcaca gacatttctt ttcagccttc ttatgcttct gcacgttgta atgtgttttt
     2161 ttctttttta cggaatgcat acagagcctg aatttgatta cagaatagag ctttatcaca
     2221 ttgcaaaatt cataatgtac gcaaccacgg taatgaacat aatcggcata acctgcctta
     2281 tgttcggctt taaatttgaa tggtactgga ttaaatttac cgtttacgaa aacaggttca
     2341 ccggttgtta tgtcaacccg aatctcctcg gcttcatatc tgtggtgtcg attttctgtt
     2401 gccatatact ttcaaaaggg cattttatgc gcagaattgc cgaaaaaatt cctgaacccg
     2461 gcatcagtaa gatatggatt gtagcctgtc ttgccacaaa tgcgttttcg ctcattctgt
     2521 gcgattcaaa cgcctcactt gtgcttgctc tcggatatgc aattgtttat atagtttata
     2581 tgttctttgc cgacaaggcg ggactttcac cttcaaaaat tattttgaaa attacagcgc
     2641 tgtttcttgt gggagttttc cttacaggct ctgccttgat gttcagaacg atttgtcagg
     2701 aaggcttttc cgttgttgta tcaaagacaa cctctgttgt cgatttgctt ttaggcaaaa
     2761 ccgaaagcga acttgtgcag gaagaactga ctcccgaaca gagagaacag cttgaaaagg
     2821 ataaaatcac cttttcacac gaaaacaaaa acattgacag cggaagaaag aagctttggc
     2881 ttgaatcaat caatcttttc aaactgtcgc cgattatcgg aatttccaac ggaaatattg
     2941 ttctctacag tgccgaatat tcaaacggtg ctttggaata ttcgtatcac aaaagtgatt
     3001 tgcacaacgg ttttcttaca attcttgttt caacgggtgt aataggtttt gttcttttcg
     3061 gaattttcgg ttttcgattt gcaaaacact ccgcacagca cctgtttttg cagaaaaaaa
     3121 catatcgtga cgatgtttat ccctgtctgt ttgcttttct tttcgcatat ttgatttttg
     3181 cctgctttga aaaggcattg ctttatgaca tatcgtttat ggtggtgtgg ttctggctga
     3241 ttatgggata tatgagttgc tacatcacaa agtttgaacc gacgcttgaa agtcagtatc
     3301 tttttcacgg aaaaagactc agacgggtaa caagaacaat gctctgatgc ttgtataatc
     3361 ggaaaatttg atgtataata accgtaggag cgttttgttt ctgcggttat tattttgtga
     3421 ggtataattt tatgaaatac ggtttcatca aggttgcatc cgcaacaccg aaaatcaggg
     3481 ttgcagactg taaaaccaac acaataaata ttattgaaca gattaaagag gcacacaaaa
     3541 acggtgcgtc actggttgtg tttcccgagc tttgcgtaac gggctacacc tgttccgatt
     3601 tgttttatca gcgtgttttg ctt
//
LOCUS       DAOMVE010000155         3622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7484_length_3622_cov_3.015980, whole genome shotgun sequence.
ACCESSION   DAOMVE010000155 DAOMVE010000000
VERSION     DAOMVE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7484_length_3622_cov_3.015980"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            183..665
                     /locus_tag="PLS64_07980"
     CDS             183..665
                     /locus_tag="PLS64_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRL43796.1"
                     /translation="MPFEEFYRQNFKTVYGFLINLCKNSDLAEELTAETFLKAFEHYK
                     SYNKKYKPSTWLCAIAKNEYLKYLRKQKKIDGFDNAEIADISNLEEKLSDKDTALRIH
                     KYLHILDEPYREVFILKVFAELSYKEIAEIFGKKETWVRVTYYRAKVRLLERLGDDNE
                     "
     gene            658..1368
                     /locus_tag="PLS64_07985"
     CDS             658..1368
                     /locus_tag="PLS64_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRL43797.1"
                     /translation="MNKNCNIVRDLIPLYSDNTASDESRKFIEEHCRTCDKCNRILSL
                     SKTEIDKTAHLDDEINSVWKSIEKQNKKKRIIKVAIAAILVTVLIPLIIIGANYMYGA
                     DNTDTAKSPYFSDEMLNEFDKGYSQSDQKQLEPLLNDIKNVIDFNGEYETAKGKFGEL
                     AYYSYDRVEGDYTVKAKVELNSAKLYTDTGYMWIEYTKDLYTEDGTFWMTTEPVKSRI
                     TVINKDGEWTAVNIQSEQ"
     gene            1429..1839
                     /locus_tag="PLS64_07990"
     CDS             1429..1839
                     /locus_tag="PLS64_07990"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005358373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRL43798.1"
                     /translation="MVREIVHDPMFLQRKSEDATQADLQTAQDLLDTLRANFDRCVGM
                     AANMIGVAKKIIAINDNGKYLVMFNPEIISKFGEFETEEGCLSLDGERKTVRYKTIKV
                     KYFNENFKQIKRSFSDFTAQIIQHEIDHCNGILI"
     gene            2596..3111
                     /locus_tag="PLS64_07995"
     CDS             2596..3111
                     /locus_tag="PLS64_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43799.1"
                     /translation="MKSNKTLKYTGIIQGVLIATLLLLAIWIQVTFLYNYYCEDSSFW
                     AIHGDYPFLFIFNTLFTTFASFSSLLENYVLYVIFEIMDIASLVLPVYLVYKSIRSKS
                     TMANGLIIISSAVTFMIMVAYGVYTLLDIIRGAETFNIYSIIFIFIMLIKVTLCIVSL
                     LRIRAINNAVE"
     gene            3317..3571
                     /locus_tag="PLS64_08000"
     CDS             3317..3571
                     /locus_tag="PLS64_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43800.1"
                     /translation="MKKFVSFLLALMMIFWSSTLAFATDKTDTQDNSTSDESDGGSMP
                     TDFAIILLLIAGGTAVASFKRHRNDRDDYDEPDDDTEEEI"
BASE COUNT         1212 a          639 c          661 g         1110 t
ORIGIN      
        1 tcaacctcag cataaacatt gcacgataac tccggatatt tcacctaacc acctcggtaa
       61 tttatatgta gacaagaggt tgtctgttgc aaaacattga acagaaaaaa tttttatgat
      121 aaaatttcaa tatactgtaa caaaacagca atttgcggat ttaatatacg ggaggtgaca
      181 aagtgccatt tgaagaattt tacagacaaa actttaaaac agtttacgga tttctgatta
      241 atctctgtaa aaacagcgac cttgcagagg aattgactgc cgaaactttt cttaaagcat
      301 ttgaacatta caaaagctac aacaaaaagt acaagccctc aacatggctt tgtgccattg
      361 ccaaaaacga atatttaaaa tacctgcgta agcaaaagaa aattgatggt tttgacaatg
      421 cagaaattgc cgacatcagc aatttagaag aaaagctttc ggataaagac acggcattgc
      481 gaatacataa atatctgcat attcttgatg aaccgtatcg tgaggtcttt attttaaaag
      541 tttttgcaga gctgtcatat aaggaaattg ccgaaatttt cggcaaaaaa gaaacatggg
      601 tacgagtcac ctattaccgt gcaaaagtca gattattgga aaggcttggt gatgataatg
      661 aataagaact gcaacatagt gcgtgattta attcctttgt attccgataa taccgcaagt
      721 gatgaaagca gaaaatttat tgaggagcat tgccgtactt gcgataaatg caatcgtatt
      781 ttatcacttt caaaaaccga aattgacaaa acggcacacc ttgacgatga aattaacagc
      841 gtatggaaaa gtattgaaaa gcagaacaaa aagaaaagaa taattaaagt tgcaattgcg
      901 gctattttgg ttactgttct tatcccctta attattatcg gggcaaatta tatgtacggt
      961 gctgacaaca cagatacagc gaaaagccca tatttcagcg acgaaatgct aaatgaattt
     1021 gacaagggat attcacaatc ggaccaaaaa cagcttgaac cgcttttgaa tgatataaaa
     1081 aatgttattg atttcaacgg tgaatatgaa accgccaaag gaaaattcgg agaacttgcg
     1141 tattattcat atgaccgtgt tgagggcgat tacacagtca aagctaaggt tgaattgaac
     1201 tccgcaaagc tttacacaga cacgggatat atgtggattg aatacacaaa ggatttatac
     1261 acagaggacg gaactttttg gatgacaacc gaacccgtaa aatcacgaat taccgtaatc
     1321 aataaagacg gtgaatggac agcggtcaat attcagtcgg aacagtaagg cttgttttaa
     1381 atacaccgat gtgttatact cgggttagaa actgaaagag gcagagttat ggtaagagaa
     1441 attgtacatg atcctatgtt tttacagaga aagtcggagg acgcaacaca ggcagatttg
     1501 cagacggcac aggatttgct tgacacactc agggcaaatt ttgaccgttg tgtgggcatg
     1561 gcggctaaca tgataggcgt tgcaaaaaag ataatcgcaa ttaatgacaa cggaaaatat
     1621 cttgttatgt tcaatcccga aatcattagc aagtttggtg aatttgaaac cgaggaaggc
     1681 tgtctttcgc ttgacggcga acgcaaaaca gttcgttaca aaacgataaa agtaaaatat
     1741 ttcaacgaaa atttcaagca gataaaacgc agtttttcag actttaccgc acagattatt
     1801 caacatgaaa ttgaccattg caacgggatt ctgatttaaa gttaatacaa atccttctct
     1861 ccccgacagt cacacggtac aatgtgtggc tgtcgggttt tattttgcac atttattttg
     1921 cacaaaagtt ttactgcaat ttattcatta caactaattt taccgtcaat ctgaaccttt
     1981 ttaggattat tgttttgtaa aaatcgcacg aaattgcatg gcagaatatt ttgcaattgc
     2041 actttgaatt acattgtgct ataatattaa tacaatagaa acacagaaaa attgtaatta
     2101 atcaaaaggc ggtgatttta tgaaagaaat tacaaaaaat caagcgacag caatttggca
     2161 gattttcttt gtattgtggc ttactgtagg gtcaatcgtg tgttttaata ttcattgtat
     2221 aacatccgag ccgccgatat acgcacctat gtttgcaata actccgcttt atctcataga
     2281 gttctatttt gaccacccct acaccgtaca ggcaattctg tttcaaaact ttgtgctgat
     2341 attgatgccc gcactgcttt ggtttttaac atacaaatca gtattcaaaa acaaaaaatc
     2401 cgcaaataca attattctta ttcttatgat tcccgacacc gttttgtcag cattttacgg
     2461 cttatttttt acaataggat attttttcac atccaatcac tattttctat tgttagcgtt
     2521 ttacataatc aatttaataa taaaaatcgg attaattttt gtaacggtta aaagagatta
     2581 aaaggcggtg agattatgaa aagcaataaa actctcaagt acacaggaat tatacaaggt
     2641 gtactcattg caactctgtt actgcttgcc atttggattc aagtgacatt tttgtataac
     2701 tactattgtg aagattcttc tttttgggca atccacggcg actacccgtt cctattcatt
     2761 ttcaacactc tttttacaac ctttgcatct ttctcaagct tgttagaaaa ttatgtttta
     2821 tatgttattt ttgaaattat ggatattgca tcgttggtat tgcccgtata tctcgtttac
     2881 aaatccatac gcagtaaaag cacgatggca aacggattga ttattatatc atctgctgtt
     2941 acttttatga ttatggttgc ttacggagtt tatacccttc ttgacataat tcgcggtgct
     3001 gaaacattca atatttacag cataattttc atcttcataa tgttgataaa agttactctg
     3061 tgcattgttt cactgctcag aataagggca attaacaacg cagtcgaata gtattacata
     3121 taaacaagac agtttacggc tgtcttgttt tgttatttat taaaattcct tcattgtata
     3181 accctttccc tacaaaatct acagggacag ttgctgtgaa cattgacata tattgctgat
     3241 ttaatatccc aaatttaata ttgctaattt ggaattataa tggtataatt cgtgtagata
     3301 ttgcggaggt gttgtcgtga aaaaatttgt atcgtttttg cttgctctta tgatgatttt
     3361 ctggagcagt acccttgctt ttgcaactga caaaacagac acacaagaca actccacctc
     3421 cgatgaatcg gacggcggtt caatgcctac ggattttgcg ataattcttc tgcttatcgc
     3481 cggcggtacg gcagtcgcaa gctttaaacg ccacagaaat gaccgtgacg attacgatga
     3541 acccgatgat gacacggaag aagaaatttg attttacatt attaaaaaca acaaagccga
     3601 cagaatacaa tcgctgtatc tg
//
LOCUS       DAOMVE010000156         3611 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7519_length_3611_cov_3.443476, whole genome shotgun sequence.
ACCESSION   DAOMVE010000156 DAOMVE010000000
VERSION     DAOMVE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7519_length_3611_cov_3.443476"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..2620)
                     /locus_tag="PLS64_08005"
     CDS             complement(74..2620)
                     /locus_tag="PLS64_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43801.1"
                     /translation="MADFGKYFRAYMYMQDILKNDFTYNYITEGLKDGDKGEDKLDGK
                     TNEKVIDMEWVVAIEETLPYIQKAIDEQRRFIKQVDNVVRVELAKKIGPESVKHLSQH
                     TNFIAKVEGDMVTPNKILTIEREESFAIYENRVLMTLIRKALHFVDDKYSKMKDVPND
                     SYNKISMVRHIDFNEKKVDFNLNYINESHETLADDLDVLDVSQLSDFDRIRRIRTTLN
                     EFLNTQLIKEISKEPEVRPPLMQTNLLKKNPNFKRVVELWNFLDSYKRKGFEVVSEEY
                     NGKMTDTVQQDVYFAMGFQHFMMSIATNPALRSILKEQYDAENARIAEEESKPQKTRE
                     AVMKAQLDAVRKEEMEIRLREIREREKKILDLTNEIKNLKVIIDQKEQQILTLRGRVS
                     ALEDQLEEVKKELQQTKLKLMEAEKRIAELEEENQQLKDTIEELNKTIDELNQTIDTL
                     NKKIVELNNRILVLVQENARLQTKVKEQETVIAEQKAHIEELETQVAEQLAKITALTE
                     NLEKCHAQIEEDARKITDLSDKNEKLTDTLQTERIQHKETVDKMNADFAEEKQQMNDA
                     HSAEVESLNHKFNDAEAEHNTYVAKLNADFAAKANETEQKHLKELADKEETHKNELVE
                     IKEANLKSSAASNAAHASELKKAYKSVDKKVELAEKACEKRMKAKVAEVKKEAQAEIR
                     KAEKKASEKISLAKDEIKSVKDTGDLFVRDYGFGSVAVVSKYAEELAKFGKLSIADEL
                     INAQKSVRSLCICRSKKGVVLAGFSQSGASVYNVYKKQADLTVAFNDLCSMLKGMSNL
                     PIVITFSGVDRPFVMNFENIVRDATKRKTTVFHNKTLKANGFVSVYFGKE"
     gene            complement(2714..>3611)
                     /locus_tag="PLS64_08010"
     CDS             complement(2714..>3611)
                     /locus_tag="PLS64_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL43802.1"
                     /translation="TRLIILQGISGTGKTSLAYAFGKFVNNPSIVASVQPSWRDRTEL
                     FGYFNEFTKKFNETELLRAMYEASYNDNIYAVILDEMNIARVEYYFAEMLSILEMPSR
                     DEWVVDIIPNSWPSDPKHIVNGQLKIPPNMWYIGTANNDDSTFAITDKVYDRAMPINI
                     DTKGVPFEAPLTDSVYISYKHFEYILNAAKVQFVVSEENLKKIALLDDYVIEHFRIAF
                     GNRIVKQLRDFVPAYVGTGGTELDGLDYVLARKVFRKFESLNLSYIRDEIDGLCAYID
                     ELFGEENMTESKDYLRMLKKLV"
BASE COUNT          945 a          798 c          675 g         1193 t
ORIGIN      
        1 gaactattaa atttttagca atcattctgc ccgtttttct gttgggcgca gtagagtttt
       61 tcatttttgc cccttattct ttaccaaagt aaactgaaac aaatccgttt gctttcagag
      121 ttttgttatg gaaaacagtt gtttttcttt tggttgcatc tcttacaatg ttttcaaaat
      181 tcatcacaaa cggtctgtca acaccggaga atgtaattac aatcggcaga ttgctcatac
      241 ctttcagcat tgaacacaaa tcgttaaatg caacagtaag atctgcctgc tttttataaa
      301 cattatatac gcttgcgccg ctctgtgaaa agcctgcaag cacaacaccc tttttacttc
      361 tgcatatgca aagagatctt acgcttttct gtgcgtttat aagctcgtct gcgattgaaa
      421 gcttgccgaa ttttgcaagc tcttcggcat attttgaaac aactgcaaca ctgccgaagc
      481 cgtagtcacg aacgaacaaa tcgcctgtat ccttgacaga tttaatttca tccttagcaa
      541 ggctgatttt ttcgcttgct ttcttttcgg ctttgcgaat ttcagcctga gcctcttttt
      601 taacctcggc aaccttggct ttcatacgct tttcacaagc tttttctgca agctcaacct
      661 ttttgtcaac cgacttataa gcctttttaa gctcagaagc gtgagcggca ttgcttgcgg
      721 ctgatgattt aaggttggct tccttaattt caacaagttc attcttgtga gtctcttctt
      781 tatctgcaag ctctttaaga tgcttttgct ctgtttcatt tgcctttgcc gcaaaatccg
      841 cattcagctt tgctacataa gtgttatgtt cagcctctgc atcgttgaat ttatggttaa
      901 gcgattcaac ctctgccgaa tgagcgtcgt tcatctgctg tttttcttca gcaaaatcgg
      961 cattcatttt gtcgactgtt tccttatgct gaatgcgttc ggtttggaga gtatcagtga
     1021 gcttctcatt tttgtcagac aaatcggtaa tcttacgagc gtcctcttca atctgcgcat
     1081 gacatttttc aaggttttcc gtaagagccg taatctttgc aagttgctcg gcaacctgag
     1141 tttcaagttc ttcgatatga gctttctgct cggcgataac agtttcctgc tctttcacct
     1201 ttgtctgcaa acgagcattc tcctgcacaa gcacgagaat tctgttattt aattctacta
     1261 tttttttatt gagtgtgtca atagtctgat tgagttcatc aatcgtttta ttcaactctt
     1321 caatagtatc ttttaactgc tgattttctt cctcaagctc ggcaattctc ttttctgcct
     1381 ccatcaactt gagttttgtc tgctggagct ctttcttaac ctcttcaagt tggtcttcaa
     1441 gagcagaaac cctgccccta agagtgagaa tctgctgttc tttctgatca attataacct
     1501 ttaaattctt gatttcattc gtgaggtcaa gaattttctt ttcgcgttca cggatttcac
     1561 gaaggcggat ttccatctcc tctttacgaa cagcatcaag ctgtgccttc ataaccgcct
     1621 cgcgggtctt ctgcggcttt gattcttcct cggcaattcg tgcattttca gcgtcgtact
     1681 gttccttaag gattgaacga agtgccgggt ttgtagcaat agacatcata aaatgctgga
     1741 atcccatcgc aaagtaaaca tcctgctgaa cagtatctgt cattttgccg ttgtattcct
     1801 cgcttacaac ctcaaagcct ttacgcttgt aactgtcgag aaagttccac agctcaacca
     1861 ctcttttaaa gttggggttc tttttgagaa ggttggtctg catgagcggc ggacgaacct
     1921 caggctcttt tgagatttcc ttaatcaact gagtgtttaa aaactcatta agggttgttc
     1981 tgattcttct gattcggtca aagtccgaaa gctgagaaac atcaagaaca tcaaggtcat
     2041 ctgcaagagt ttcgtgagat tcatttatat agttaagatt aaaatctact tttttctcat
     2101 taaaatcaat gtgacgaacc atagaaattt tattatagct gtcgttcggc acatctttca
     2161 tttttgaata tttatcgtca acaaagtgaa gcgctttgcg aatcagcgtc ataagcacac
     2221 ggttttcata aatagcaaag ctttcttcgc gctcgattgt gaggattttg ttaggcgtta
     2281 ccatatcacc ctcaaccttg gcaataaagt tagtgtgctg cgaaaggtgc ttaaccgact
     2341 cggggccaat cttttttgca agctcaactc taacaacatt gtcaacctgc ttaataaaac
     2401 ggcgctgttc gtctattgct ttctgaatgt acggaagtgt ttcctcaatg gcaacaaccc
     2461 attccatatc aattaccttt tcgttggtct taccgtcgag cttatcctcg cccttatcgc
     2521 cgtccttaag gccttctgtt atgtaattgt aagtaaaatc atttttcaga atgtcctgca
     2581 tatacatata tgctctgaaa tatttaccaa aatcagccat tgatttttct ccaatcagat
     2641 agttttaccg ctaaaggaac aaagtccttt caccaagcaa agggcgaaca gagttcgccc
     2701 tgaattgcta aaattataca agcttcttga gcatacggag ataatccttg ctctcagtca
     2761 tattttcttc gccgaaaagc tcgtcaatat acgcacaaag gccatctatt tcatcacgaa
     2821 tgtatgaaag attgagtgac tcaaattttc taaatacctt gcgggcaaga acatagtcaa
     2881 gaccgtcaag ctctgtaccg cctgttccga cataagcagg aacaaagtca cgaagctgtt
     2941 ttacaatacg gttaccaaaa gctatacgga aatgctcaat aacataatca tccagaaggg
     3001 caatcttttt gaggttctct tccgaaacaa caaattgaac cttagcggca ttgaggatgt
     3061 actcaaaatg tttgtatgaa atgtatacag agtctgtgag aggtgcttca aaaggaacac
     3121 cctttgtgtc gatgttaatc ggcatagcac ggtcataaac cttatctgtg atagcaaatg
     3181 tcgagtcatc gttattcgca gtaccgatgt accacatatt cggcggaatt ttgagctgac
     3241 cgttcacgat atgcttcggg tcagacggcc aagagttcgg gatgatatca acaacccact
     3301 catctcttga cggcatttca agaattgaaa gcatttctgc aaagtaatac tcaactcttg
     3361 cgatgttcat ttcatcaagg attacagcat agatgttatc attgtaggaa gcctcgtaca
     3421 ttgcacggag aagctctgtt tcgttaaact tcttagtaaa ttcgttgaag taaccgaaaa
     3481 gctctgttct gtcacgccat gacggctgaa cagatgcaac gatggacggg ttgtttacaa
     3541 acttaccgaa tgcatatgca agtgaagttt tacctgtacc ggaaataccc tgaaggataa
     3601 taagtcttgt t
//
LOCUS       DAOMVE010000157         3602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7542_length_3602_cov_1.759797, whole genome shotgun sequence.
ACCESSION   DAOMVE010000157 DAOMVE010000000
VERSION     DAOMVE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7542_length_3602_cov_1.759797"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLS64_08015"
     CDS             complement(<1..830)
                     /locus_tag="PLS64_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HRL43803.1"
                     /translation="MININETNKKHCCGCTACESVCHKKCISMQTDNEGFKYPVVDKT
                     KCVNCRLCEKVCPILNKTHNVFPNTAYVVRNKNSEIVRSSTSGGAVTAFSKEIINNGG
                     IVFGGAFDGNFYVKHTYAETESELSKFRSSKYVQSDMGTTFTEVKQQLDNGKFVMFTG
                     TPCQVEGLQNYLRKPYDNLFTIDFVCRAVPSPLVWEKYREFMEKKFGSEITYANFREK
                     TYGYHSANLTLRFANGKKSIENTNTDYMLKSFFKGICSRPSCYDCKFREPGRVSDLTV
                     "
     gene            complement(832..>1077)
                     /locus_tag="PLS64_08020"
     CDS             complement(832..>1077)
                     /locus_tag="PLS64_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRL43804.1"
                     /translation="MLYKKQFSIFNRYSNDSVSSKNSRIDSFCETYGLNDRRFSGNIN
                     DVENAIDYDCVLEKVKNHRMLSKAFLDNALSCFSNGD"
     assembly_gap    1078..1087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1088..>1542)
                     /locus_tag="PLS64_08025"
     CDS             complement(<1088..>1542)
                     /locus_tag="PLS64_08025"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRL43805.1"
                     /translation="TKISYATSFGVSQIEQRYHKIYKNFLDRLNYVSVRENSGKVIVD
                     KLSINDAVVVCDPVILLDSDEWLEEIPNKNLFNEPYIFAYFLGKSQEYRDEVTKFAKE
                     KNLKIVTELHMDSYNKADRGFGDYTPFDIGPAEFVNLIRNAKYIFTDSF"
     assembly_gap    1543..1583
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1584..1961)
                     /locus_tag="PLS64_08030"
     CDS             complement(<1584..1961)
                     /locus_tag="PLS64_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRL43806.1"
                     /translation="MKKLGLCACYNHKNYGSQLQSYATTVELKKRNIDYEIIRYKKKI
                     TPLLLTKAIPRLFNPVFINDRIIETFQKKLMLKLHSQLAEQNAVRNDAFNKFSENRFK
                     KLSPIYYGYDELKKQSEKYTAVMV"
     gene            complement(1966..3213)
                     /locus_tag="PLS64_08035"
     CDS             complement(1966..3213)
                     /locus_tag="PLS64_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRL43807.1"
                     /translation="MKILLVNKYHYVKGGSETYYFGLADLLTKLGHEVIYFSMADINN
                     KPCEQEKYFVSNVDFNSNISTLQKVKAGFRVLYSFEAKKNISALIEQEHPDIVHINLV
                     HRHITLSIVRAIKKYNIPIVFTIHDLNCVCPNHEMLVNGHVCEKCLHGRYSNCIKNKC
                     VKGSTAKSTLAAIEATNYKRMKIYDDIDLYITPSYFYKKKLEESGIIHSEIIHIKNFL
                     PTDTVYSDNNPDNGYLLYFGRISEEKGVLTLVKAVEKLENNIPLIILGTGPIEDEIKE
                     YIKAHNLGNRIKALGFKSGDELKKYVAEAKCVILPSEWYENGPYTIMEAMSQGKPVIV
                     TNYGGLPEIVEDGKTGFICSPFDSDDLKNSIKKVCSLSDAEYKAMSSCAVEKAKIEFS
                     PVTYAQKLTQKYNEIINNHKKKG"
     gene            complement(3216..>3602)
                     /locus_tag="PLS64_08040"
     CDS             complement(3216..>3602)
                     /locus_tag="PLS64_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43808.1"
                     /translation="KYNAMTVFFDFNFKDLMKVRRPEHHLSEEEYIEAVTDPYIIHYT
                     SCFLSGTRPWNENNNHPFVGDYLKYKSMSPWKDFPQYPDDRKKSQKMMTSVCNAMPKR
                     IMIAGITLVHSKLYPMVRSLKGKRRG"
BASE COUNT          984 a          652 c          663 g         1252 t
ORIGIN      
        1 aatacagtca gatccgatac tcttcccggc tcacgaaatt tacagtcata acatgatggg
       61 cgtgagcata ttcccttaaa aaagctctta agcatatagt cagtattggt gttttctata
      121 ctttttttgc cgttggcaaa tctaagagtc agatttgcac tatggtatcc gtaggttttt
      181 tcgcgaaagt ttgcatatgt aatttcagaa ccgaattttt tttccataaa ttccctgtat
      241 ttttcccata ccaatggaga tgggacagct ctgcatacaa agtcaatggt gaagaggtta
      301 tcatatggtt ttcttaagta attttgaagc ccttcaacct gacacggagt ccccgtaaac
      361 ataacaaatt ttccgttgtc aagttgttgc tttacctcgg taaaggttgt acccatatca
      421 ctttgaacat attttgaact tctgaatttt gaaagttcac tttctgtttc agcgtatgta
      481 tgtttgacat aaaagttacc gtcaaatgca ccaccaaata cgattccccc gttattgatt
      541 atctctttgc tgaaagcggt aactgcaccg cctgatgtac tggagcggac aatttcagaa
      601 tttttattac gaacaacata tgcggtattt ggaaatacat tatgtgtttt attgagtata
      661 ggacaaacct tttcacaaag gcgacagtta acacatttag ttttatcgac aaccggatat
      721 ttaaatccct cgttgtctgt ctgcattgat atgcactttt tatggcacac agactcacat
      781 gctgtacatc cgcaacaatg ttttttattt gtttcgttta tatttatcat tttaatctcc
      841 gtttgagaaa caactgagtg cattgtcaag aaatgctttg gacagcattc tgtgattttt
      901 aactttttcg agaacacaat cataatctat tgcattttca acatcgttta tattaccgct
      961 gaatcttctg tcgtttaatc cgtatgtctc acaaaagctg tcaatacggg agtttttgga
     1021 agatacagaa tcgtttgaat agcggttgaa tatagaaaac tgttttttgt acagcatnnn
     1081 nnnnnnngga aagaatcggt aaatatatac ttagcatttc taatcaaatt gacaaattca
     1141 gccggtccga tgtcaaacgg tgtgtaatca ccaaaacccc tgtcagcctt attataactg
     1201 tccatatgca attctgtgac gatttttaaa tttttttctt ttgcaaactt ggttacttca
     1261 tctctgtatt cctgactttt accgagaaaa tatgcaaata tatacggttc attgaaaaga
     1321 ttcttgtttg gaatttcttc gagccattca tcggaatcaa gaagaattac agggtcacag
     1381 acgacgacgg catcatttat gctgagcttg tcaacaatta cctttccgct gttttctctg
     1441 acgcttacat aattaagtcg gtcaagaaag tttttgtaaa ttttatggta tctttgctca
     1501 atctgtgaaa caccgaagct tgttgcatat gatatttttg tgnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnaaccata acggcggtgt atttttcaga ttgcttctta
     1621 agttcatcgt aaccgtagta aataggtgaa agctttttaa atctgttttc cgaaaattta
     1681 ttaaacgcgt catttctaac ggcattctgt tccgcaagct gtgaatgaag tttaagcata
     1741 agcttttttt ggaatgtttc aataatcctg tcatttataa aaacggggtt aaaaagacgc
     1801 ggtattgctt ttgtaagaag caacggtgta atcttttttt tatatcggat tatttcgtaa
     1861 tcaatgttac gctttttcag ttcaactgtt gttgcatagc tttgaagctg gctgccgtag
     1921 tttttgtggt tgtagcaagc gcaaaggcca agttttttca tggtatcagc ctttcttttt
     1981 gtgattattt ataatctcat tatatttttg tgtgagtttc tgcgcatagg ttaccggaga
     2041 aaattcgatt tttgctttct caacggcaca actgctcatt gctttgtatt cagcatcaga
     2101 aagcgagcag acttttttaa ttgagttttt aaggtcatca ctgtcaaacg gtgagcagat
     2161 aaagcctgtt tttccgtctt ctacaatttc aggaagtccg ccgtaatttg ttacgataac
     2221 aggttttccc tgtgacattg cttccataat tgtatagggt ccgttttcgt accactcact
     2281 gggaagaatg acgcatttcg cttctgcaac atacttttta agctcgtcac cggacttaaa
     2341 gcctaatgct ttgattctgt ttccaaggtt atgtgctttt atatattcct tgatttcatc
     2401 ttcaatagga cctgtgccga gaatgatgag cggaatattg ttctcgagct tctcaacagc
     2461 cttaacgagt gtcagcacac ccttttcctc ggaaattctg ccaaagtata acagataacc
     2521 gttgtcggga ttattgtcgg aataaacagt atcggtaggc agaaagtttt ttatatggat
     2581 aatttcgctg tggataatac cggattcttc aagcttcttt ttgtaaaaat atgacggcgt
     2641 gatgtacaag tctatgtcgt cgtatatttt cattcgttta tagtttgttg cctcaatagc
     2701 cgcaagagta ctttttgcag ttgaaccttt tacacattta ttttttatgc aatttgaata
     2761 tctgccgtgc aggcattttt cacacacatg tccatttaca agcatttcgt ggttgggaca
     2821 tacacagtta aggtcgtgaa ttgtgaacac tattggtatg ttgtatttct ttattgctcg
     2881 aacaatcgaa agggtgatgt gacggtgaac aaggttgata tgaacaatat caggatgttc
     2941 ttgttcaatg agagcagaaa tatttttttt cgcctcaaaa gaatagagaa cgcgaaaacc
     3001 tgcttttacc ttctgaagcg tggaaatatt agaattaaaa tcaacatttg aaacaaagta
     3061 tttttcctgt tcacatggtt tgttgttaat gtctgccatt gaaaagtaaa tcacttcgtg
     3121 accaagcttt gtgagaaggt cggcaagacc gaaatagtag gtttcacttc cgccttttac
     3181 atagtggtat ttattaacaa gcagaatttt cattatcaac cccgcctttt tcctttaagt
     3241 gagcgtacca ttgggtacaa ctttgagtga acaagtgtga ttcctgcaat cattattctt
     3301 ttcggcatgg cattgcacac acttgtcatc attttttggg atttctttct gtcatcggga
     3361 tattgcggaa aatctttcca cggtgacatt gatttatatt tgagataatc cccgacaaaa
     3421 ggatgattgt tattttcatt ccatgggcga gttcccgaaa gaaaacacga ggtgtagtga
     3481 attatataag gatcggttac cgcctcaatg tattcttctt cactcagatg atgctcggga
     3541 cgacgcacct tcataagatc tttaaaatta aaatcaaaga aaacagtcat agcgttgtat
     3601 tt
//
LOCUS       DAOMVE010000158         3594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7564_length_3594_cov_3.171800, whole genome shotgun sequence.
ACCESSION   DAOMVE010000158 DAOMVE010000000
VERSION     DAOMVE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7564_length_3594_cov_3.171800"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /gene="nrdD"
                     /locus_tag="PLS64_08045"
     CDS             <1..1964
                     /gene="nrdD"
                     /locus_tag="PLS64_08045"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRL43809.1"
                     /translation="MAKGAFELARRYVRYRYNRSLVRKANTTDNRILSLIECNNEEVK
                     QENSNKNPTVNSVQRDYMAGEVSRDLTRRMLLPADIVEADKEGIIHFHDSDYFAQHMH
                     NCDLVNLEDMLQNGTVISDTLIERPHSFSTACNIATQIIAQVASNQYGGQSISLAHLA
                     PFVQVSREKIRRETLAEIEELGVHPTEEKINDIVEQRLRKEVNRGVQTIQYQVVTLLT
                     TNGQAPFVTVFMYLNEAKNEQEKHDLAMIIEETLKQRHKGVKNEKGVWITPAFPKLIY
                     VLEEDNITEDSKYWYLTELAAKCTAKRMVPDYISEKVMLQSKIDKNGEGHCFTCMGCR
                     SFLTPYVDENGKPKYYGRFNQGVVTVNLIDIGLSAGKDLDKFWKIFDERMELCHRALQ
                     CRHERLTGTLSDAAPILWQYGALARLKKGEKIDKLLHGGYSTLSLGYAGLWECVYSLI
                     GKKLTEKEGKELGLEIMQKLNDYCAKWKKAENIDYSLYGTPLESTTYKFAKCLQKRFG
                     IIKGVTDKNYITNSYHVHVTENIDAFSKLKLESEFQALSPGGAISYVEVPNMQDNIPA
                     VLQVIKFIYDNIMYAELNTKSDYCQVCGYDGEIKIVHDDGKLVWECPNCGNRDQDKMN
                     VARRTCGYIGTQFWNQGRTQEIEERVLHL"
     gene            1964..2500
                     /gene="nrdG"
                     /locus_tag="PLS64_08050"
     CDS             1964..2500
                     /gene="nrdG"
                     /locus_tag="PLS64_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRL43810.1"
                     /translation="MNYGEIKLCDIANGEGVRVSLFVSGCTHHCKNCFNPQTWSFDYG
                     EPFTKEVEDKILKELEPSYIDGLSLLGGEPFEPSNQRALVPFLKRVREKYSDKTIWCY
                     SGYLFDKELLSDSRARCECTDEMLSMIDVLVDGEFVEALHDISLAFRGSSNQRIIDVK
                     ASLKENKIIPHKLMKRGV"
     gene            2639..3352
                     /locus_tag="PLS64_08055"
     CDS             2639..3352
                     /locus_tag="PLS64_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HRL43811.1"
                     /translation="MVCKNCNHEIDNGAMFCTYCGTPVEQTPEPETQPVENASTEDAA
                     QNPYENVQTAQPEVDNAQPTADSQPQQNPYSQQYGQPNQTYYQQPQPNKTSYGQQFTN
                     SPNNDYGANQSKYGNQYQSYNNGYNQQYNTQYQQTYNSQPVPPVAPPYGTPDTQPVSI
                     GGWIGVMLLTCLPIVNLIMLFVWAFSSSTKKSLKNYARAALILALIGVVLVVVISIIL
                     AVCGISIFNGLSNSGYYYH"
BASE COUNT         1095 a          737 c          806 g          956 t
ORIGIN      
        1 taatggcaaa gggtgcgttt gagcttgcaa gaagatatgt gcgttaccgc tacaatcgtt
       61 cgcttgttcg taaggcaaat actaccgata acagaattct ttcgcttatc gagtgcaata
      121 acgaggaagt aaagcaggaa aattcaaata agaaccctac cgtcaacagc gttcagcgtg
      181 actatatggc cggtgaggtc agtcgtgacc ttacaagaag aatgcttttg ccggctgata
      241 ttgttgaggc tgataaggaa ggtattattc acttccacga ttcggactat tttgcacagc
      301 atatgcacaa ctgcgacctt gtaaaccttg aggatatgtt gcagaacggt accgttatca
      361 gcgatacgct tattgaaaga ccgcacagct tttcaacggc gtgtaacatt gcaactcaga
      421 ttattgcaca ggttgcaagt aaccagtacg gcggtcagag cataagcctt gctcatcttg
      481 caccgtttgt tcaggtttcc cgtgagaaaa tccgcagaga aactcttgcg gaaatcgaag
      541 aactcggtgt tcatcccaca gaagagaaaa tcaacgatat cgttgagcaa agacttcgca
      601 aagaagtaaa ccgtggtgtt cagaccattc agtatcaggt tgttactttg ctcacaacaa
      661 acggtcaggc tccgtttgta accgtgttta tgtatcttaa cgaggctaaa aacgagcagg
      721 aaaagcacga cctcgccatg attattgaag aaacactcaa acagcgtcac aagggcgtaa
      781 aaaatgaaaa gggtgtgtgg attacacctg cgtttccaaa gcttatttat gtgcttgagg
      841 aggataacat aacagaggac agcaagtatt ggtatcttac ggagcttgct gcaaaatgta
      901 ccgcaaagag aatggttccc gactatatct cggagaaggt tatgctccaa agcaaaattg
      961 ataaaaacgg tgagggacat tgctttacct gtatgggttg cagaagcttc cttactcctt
     1021 atgttgacga aaacggcaag ccgaaatatt acggcagatt caatcagggt gttgtgactg
     1081 taaatcttat tgatatcgga ctcagcgcag gcaaggatct tgataaattc tggaagattt
     1141 ttgacgagag aatggaactt tgccacagag cattgcagtg ccgtcacgag cgtcttacag
     1201 gcactctttc ggatgccgct cctattttgt ggcagtacgg tgctttggca agacttaaaa
     1261 agggtgagaa gattgacaag cttctccatg gcggttattc aacgctttca ctcggttatg
     1321 caggtctttg ggagtgcgtt tacagcctta tcggcaaaaa actcaccgaa aaagagggca
     1381 aagaactcgg ccttgagatt atgcagaaac taaacgacta ttgtgcaaag tggaaaaagg
     1441 ctgaaaacat tgactacagc ctttatggca ctcctcttga aagcacaaca tataagtttg
     1501 caaagtgtct tcaaaagcgt ttcggcatta tcaagggcgt aacagataaa aactacatca
     1561 ctaacagcta tcatgttcat gttaccgaga atattgacgc attcagcaag ctgaagcttg
     1621 aatcggaatt tcaggctttg tcgccgggcg gtgcaatcag ctatgttgaa gttccgaata
     1681 tgcaggataa cattcccgct gttttacagg ttatcaagtt catttacgat aacattatgt
     1741 atgcagagct taacacaaag agcgactact gtcaggtttg cggttatgac ggagaaatca
     1801 agatcgttca cgatgacggc aagcttgtgt gggagtgtcc taactgcggc aaccgtgatc
     1861 aggataagat gaatgttgca agaagaacct gtggatatat cggaactcag ttctggaatc
     1921 agggcagaac tcaggaaatt gaggaaaggg tgcttcacct ttgatgaatt acggagaaat
     1981 taaactctgc gatattgcaa acggtgaggg agtaagagta tcgctctttg tatccggctg
     2041 tacgcatcat tgcaaaaact gctttaatcc gcagacatgg agttttgatt acggcgaacc
     2101 ttttacaaaa gaggttgagg ataaaattct caaagagctt gaaccgtcat atattgacgg
     2161 cttgtcattg ctcggcggag aaccctttga accttcaaat caaagagcct tagtgccgtt
     2221 tttaaagcgt gtcagagaaa aatattccga caaaacaatt tggtgttatt cgggctattt
     2281 gtttgacaag gagcttcttt ccgacagcag agcaagatgt gaatgtacgg atgaaatgct
     2341 ttcaatgatt gatgtgcttg ttgacggtga atttgttgag gctcttcacg atataagcct
     2401 tgcgttcaga ggctcgtcaa atcagcgtat tatagatgta aaagcgtcac tcaaagaaaa
     2461 taaaataatt ccccacaagc ttatgaaaag gggagtgtaa taaaaagcga cagccgtaaa
     2521 actgtcgctt tttgtaatat tattgaatta ttgcgtggaa tgttgttgat tttgtaaggc
     2581 aaatagtgta aaatgatagc atactattta tagaatatta tgcaaggagc gattatatat
     2641 ggtatgtaaa aattgcaatc acgaaattga caacggagca atgttctgta cttactgcgg
     2701 aacacctgtt gagcagactc cagagcctga aacacagcct gttgaaaatg cttcaaccga
     2761 agatgctgcg cagaaccctt acgagaatgt gcagactgca cagcctgagg ttgataacgc
     2821 acagccgact gccgacagtc agccacagca gaatccgtat tctcagcagt acggtcagcc
     2881 aaatcaaact tattatcagc aaccgcagcc taacaaaact tcatacggtc agcagtttac
     2941 aaactcaccg aacaatgatt acggcgcaaa tcaaagcaag tacggcaatc aatatcagag
     3001 ctacaacaac ggctataatc agcagtacaa cacgcagtat cagcagacat acaactcaca
     3061 gcctgtaccg cccgttgcac cgccatacgg tactccggat actcagcctg taagcatagg
     3121 cggttggatc ggcgttatgc ttttaacctg tctgccgatt gtaaacctca ttatgctctt
     3181 tgtctgggca ttcagctcat caacaaagaa gtcactcaaa aactatgcaa gagccgctct
     3241 tatcctggct cttatcggag ttgtgcttgt ggtagttatt tcaattatac ttgcagtctg
     3301 cggtatcagc atcttcaacg gactttccaa ttcaggatat tactatcatt aaatttaacg
     3361 gtgaatcgct gtaccaaatt cgtacagacc gctttataat gttaaaaaca aagtttaccg
     3421 atacaaaaaa atccctgtcc ataacggaca gggattttca tttgcgttat aaaaaagttt
     3481 tagggttgcg aacctcggct cttctaatgt caagcctcgt tgttctccgc tattcctaaa
     3541 ataacttcca ctcgtctaat ctgtttcaat tacttgcgta attttcacgg ctta
//
LOCUS       DAOMVE010000159         3522 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7753_length_3522_cov_2.525526, whole genome shotgun sequence.
ACCESSION   DAOMVE010000159 DAOMVE010000000
VERSION     DAOMVE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7753_length_3522_cov_2.525526"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="queA"
                     /locus_tag="PLS64_08060"
     CDS             <1..586
                     /gene="queA"
                     /locus_tag="PLS64_08060"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRL43812.1"
                     /translation="MPLPPYITAELKDKERYQTVYSHELGSAAAPTAGLHFTTELMDR
                     IKAKGVKIAYVTLHVGLGTFRPVKVDDVTKHKMHSEHYEVPEKTAKLINETKKNGGRV
                     IAVGTTSCRTLESVAAMYGEIKPCEGFTDIFIYPGFEFKVLDGLITNFHLPESTLIML
                     VSAFAGYDFIMNAYKEAVKEKYRFFSFGDAMFIS"
     gene            604..1734
                     /gene="tgt"
                     /locus_tag="PLS64_08065"
     CDS             604..1734
                     /gene="tgt"
                     /locus_tag="PLS64_08065"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRL43813.1"
                     /translation="MFKVIKKEGSARRGEFVTVHGTVQTPAFMNVATCGAIKGAVSAL
                     DLKNIKCQVQLCNTYHLHLRPGDEKVKQMGGLHKFTRWNGPILTDSGGFQVFSLAKLR
                     NIKEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPSPYEYTKNSVE
                     RTTRWLKRCVTEMKRLNSLDDTINKNQMLFGINQGGIYDDLRVNHMKEIAELNLDGYA
                     IGGLAVGEPAETMYHIIEEVEPFAPKDKIRYLMGVGTPVNILESVARGVDLFDCVMPS
                     RNARHGQLFTWDGVRNINNAKYETDESPIDEHCDCPVCRNFSRAYIRHLLKSGEMLGM
                     RLAVLHNLYFYNNLMEVIRTQLDNGTYMEFYEKYRNILGVRI"
     gene            1818..2240
                     /gene="yajC"
                     /locus_tag="PLS64_08070"
     CDS             1818..2240
                     /gene="yajC"
                     /locus_tag="PLS64_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRL43814.1"
                     /translation="MTNFLPVVAEASASGGNQSSSMIFMILFYVAIFAALYFFLIRPN
                     SKKKKEEAQLRNSLEIGDEITTIGGIMGRVVAIKDDEDAIIIETGSDRVKMKFKKWCI
                     STVDTVKEQSQQAGQPEKKGFFGFGKKKDSDETKATKE"
     gene            2305..2697
                     /locus_tag="PLS64_08075"
     CDS             2305..2697
                     /locus_tag="PLS64_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HRL43815.1"
                     /translation="MSDKKLLAKAVLFSSVCGILILVILMCLLAAVMMSSGVISADIL
                     DYCTVGMVGVATLMSGIISSRITGSAGLAVGSLTGFFIFLVITSIALIFNGEPITVLT
                     LLKLMSSVLGGGIGGIIGVNKKEKIKIR"
     gene            2929..>3522
                     /locus_tag="PLS64_08080"
     CDS             2929..>3522
                     /locus_tag="PLS64_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRL43816.1"
                     /translation="MAKTIKLPADLRDWKVTSFVGEDNGCEVYKVSRKIDKNTAQNAI
                     LRHAFVGKSNYTDEHAEYFTEEADFIESVKELDGVSNYLDVYVQDNQNKKTCDLYIFT
                     EELTSLEELMKTKTFAEDEVVDFGIQMSEMLETLEAKNIFHGNISPKNIFVTADGRYK
                     IGGFTDFEGKIADNSFVAPEVYKQENVDYTTDIYSVGI"
BASE COUNT         1112 a          599 c          796 g         1015 t
ORIGIN      
        1 gatgcctctt cctccttata ttacggccga gcttaaggat aaggaacgct atcagacggt
       61 ttacagccat gaactcggtt ctgcggcagc tccgacggca ggacttcact ttacaaccga
      121 gcttatggac agaatcaagg ctaagggtgt aaagattgcg tatgttacac ttcatgtggg
      181 actcggtaca ttcagacccg ttaaggttga cgatgttaca aagcataaaa tgcacagcga
      241 gcattatgaa gtccccgaaa aaaccgcaaa gctcataaat gaaactaaga aaaacggcgg
      301 acgggttatt gcagtcggca caacatcctg cagaacgctt gaaagtgttg cggcaatgta
      361 cggagaaatc aaaccctgtg agggatttac ggatattttt atttatcccg gatttgaatt
      421 taaggtgctg gacggactta ttactaactt tcatcttccc gaaagtacac tcattatgct
      481 tgtatccgct tttgcgggat atgattttat tatgaatgca tacaaagaag ctgtaaagga
      541 aaagtacaga ttcttctctt tcggtgacgc tatgttcata tcataggagg attttacgga
      601 tttatgttta aggtaattaa aaaggaaggc agcgcaagac ggggcgaatt cgttacggtt
      661 cacggtacgg ttcagactcc tgcctttatg aatgttgcaa catgcggtgc aatcaagggt
      721 gctgtttctg cgcttgattt aaagaatata aaatgtcagg ttcagctttg caacacctat
      781 catctgcatc ttcgtccggg tgatgaaaag gttaagcaaa tgggcggact tcacaaattt
      841 acaaggtgga acggtcctat ccttaccgac agcggcggat ttcaggtgtt ctctcttgca
      901 aagttgagaa atattaaaga agaaggcgtt tacttcaatt ctcacattga cggccgcaaa
      961 atttttatgg gacctgagga gagtatgaga atccagtcaa atcttgcatc tacaattgcg
     1021 atggcatttg acgaatgcgt tgaaaaccct tctccatatg aatatacaaa aaacagcgtt
     1081 gaaagaacca caagatggct taaaagatgc gttacagaga tgaaaaggct caacagtctt
     1141 gatgatacca taaataaaaa ccagatgctg ttcggcataa atcagggcgg tatctatgac
     1201 gatttgcgcg tcaatcatat gaaagaaatt gcagagctta atcttgacgg ctatgcaatc
     1261 ggaggtcttg ccgtgggtga gcctgcagaa acgatgtacc atatcattga agaggttgag
     1321 ccgtttgctc cgaaagataa aatcagatat cttatgggag tcggaactcc cgtgaatatt
     1381 cttgaaagcg ttgcaagagg tgttgatctt tttgactgtg ttatgccgag cagaaacgca
     1441 agacacggtc agctcttcac ttgggacgga gtgcgtaaca tcaataatgc aaaatatgaa
     1501 acagatgaat ctccgattga cgagcattgt gactgccctg tttgccgaaa cttttcaagg
     1561 gcatacataa gacatcttct caagagcggt gagatgctcg gaatgcgact tgctgttttg
     1621 cacaacctct atttttacaa taatcttatg gaagttatca gaactcagct tgataacggc
     1681 acatatatgg aattttacga gaaatatcgc aacattttag gcgtgcgtat ctaatatttt
     1741 ttcaaaacag aaaaaatact tgcaagatgt gattaaaact gatataatca atacattgta
     1801 tgaaaggaat gacgaaaatg acaaactttt tacctgtagt tgcagaagca agtgcaagcg
     1861 gcggaaatca gtcatcaagt atgattttta tgattctctt ctatgttgct atttttgcag
     1921 ctctctattt cttcctcatc agacctaact caaagaagaa aaaagaagaa gctcagcttc
     1981 gtaactctct tgagatcggt gatgaaatta caacaatcgg cggaattatg ggcagagtag
     2041 ttgccattaa ggacgacgag gacgctatca tcattgaaac aggctccgac agagttaaga
     2101 tgaagtttaa aaagtggtgt atttctactg ttgacacagt taaggaacag tcacagcagg
     2161 caggtcagcc tgaaaagaaa ggcttcttcg gttttggcaa gaagaaagat tctgacgaaa
     2221 ctaaagctac aaaagaataa ctttttgaat gaatgttctt tcccccgaat atatttgtaa
     2281 cagaatatat tcgggggttg ttttatgtct gataaaaaac tacttgcaaa ggctgttttg
     2341 ttttcatcag tatgcggaat actcatactg gtaattttga tgtgtttgct tgcagccgtg
     2401 atgatgtcgt cgggagtgat ttcagcagat atacttgatt actgcacggt cggcatggtc
     2461 ggtgttgcaa cgctgatgtc gggaataata tcatcaagaa ttacaggaag tgcaggtctt
     2521 gcggtgggtt cgctgacagg attttttatt tttcttgtca taacctctat tgcattgatt
     2581 tttaacggtg aacctataac ggtactaacc ctgctaaaac tgatgtcatc cgttctggga
     2641 ggaggaatag gcggaattat cggagtcaac aaaaaggaaa aaataaaaat cagataagat
     2701 cgagtaagat attttactga aattatgttt tcggcagttc gtttaggcgg actgctgttt
     2761 ttatgtcgga tttaattaca aattagaatt attttttaaa aaattgaaat atctatggta
     2821 aaacagctcg taatatggta caataatccg gagaatgaga tagtgagggt taatgacctt
     2881 gatttagata taatatgaaa aataaggaaa tatttaagga gtgttagtat ggcaaaaacg
     2941 attaaattgc cggcagattt aagggattgg aaggttactt catttgttgg ggaagacaac
     3001 ggctgtgaag tttacaaggt atcaagaaag attgataaaa atactgctca gaatgcgatt
     3061 ctcagacacg catttgtcgg caaaagcaat tataccgatg aacatgccga gtatttcacc
     3121 gaagaggctg attttattga atcggttaag gagcttgacg gtgtttcaaa ctatttggat
     3181 gtttatgttc aggataatca gaacaaaaaa acctgtgatt tgtacatatt tacagaagaa
     3241 ctcacaagtc ttgaagaact tatgaaaacc aaaactttcg ctgaggatga agttgttgat
     3301 tttggtattc agatgagtga aatgcttgaa actttggaag caaagaatat ttttcacggt
     3361 aatatttcgc caaagaatat ttttgtaact gctgacggaa ggtataaaat cggcggattt
     3421 actgattttg aaggcaagat tgcggataac tcatttgttg cacctgaggt ttacaagcag
     3481 gaaaatgttg actataccac cgacatttat tctgtcggaa ta
//
LOCUS       DAOMVE010000160         3517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7779_length_3517_cov_3.172733, whole genome shotgun sequence.
ACCESSION   DAOMVE010000160 DAOMVE010000000
VERSION     DAOMVE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7779_length_3517_cov_3.172733"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /locus_tag="PLS64_08085"
     CDS             complement(<1..852)
                     /locus_tag="PLS64_08085"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRL43817.1"
                     /translation="MPDSQKISEKQSEYMNLIAEIMDIRKRGEKPVAYIRTYGCQQNV
                     ADSEKIKGMLEQSGFEFADEPDNADFILFNTCAVREHAEDRVFGNVGALKNLKRKHPQ
                     ILIALCGCMMEQEHVANKIYKSFPFVGLVFGTHSLHHFPELMYNALLDGKRVFERGND
                     DNQLYEGFPVKRDGTFKGWLPIMYGCNNFCTYCVVPYVRGRERSREKNIIVSEAKNMI
                     ESGYKDITLLGQNVNSYGKGLSEKVNFSQLLREVDSIDGDYWLRFMTSHPKDCSKELI
                     DTIASGTH"
     gene            complement(941..1594)
                     /gene="yunB"
                     /locus_tag="PLS64_08090"
     CDS             complement(941..1594)
                     /gene="yunB"
                     /locus_tag="PLS64_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HRL43818.1"
                     /translation="MLYRKRCKRKFVLLKKMLLSVVLLIIGVTVFCEVHLSDFRPEYI
                     RIQAEILSVNSVCDAVNNTLDKLNYSYSDIAKINCDKNGNVQSIETDSFKINRIKADI
                     TKAVQKEIAKVYDNEIEIPLGAFTNITILSNVGPCIPVNFNLTGSFSSEVISTFEQAG
                     INQTIHHIKLLLTSKIMTTSLDYSGKITFTTDFEIAQSVIVGNIPSGYGSLFQTYKK"
     gene            complement(1642..2130)
                     /locus_tag="PLS64_08095"
     CDS             complement(1642..2130)
                     /locus_tag="PLS64_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43819.1"
                     /translation="MNLTEKISYIRGLCDGLELDESKPEVKVLNAIVDLLDDMAFEVS
                     DMEELYDELSAQVDEIDQDLADVESDVYDDEDYDIEDYDDEDDDYDFDDEENPFYEVT
                     CDSCGQKLNVSEDVLLEGEIECPNCGENLEFDFSELFSNEEDDECDADCASCDGCSDT
                     EE"
     gene            complement(2151..2708)
                     /gene="efp"
                     /locus_tag="PLS64_08100"
     CDS             complement(2151..2708)
                     /gene="efp"
                     /locus_tag="PLS64_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRL43820.1"
                     /translation="MISAGDFRNGVTFEMDGQVVSIIEFQHVKPGKGAAFVRTKIRNV
                     ITGAVVEKTFNPNDKYPTAFIDRKDMEYSYSDGDLYYFMDTETWEQIPINKSVLGDNF
                     KFVKENMVCKVLSYKGNVFGIEPPNFVELKVTKTDPGFKGDTATNATKPATLETGAEI
                     KVPLFIDEGEVIQIDTRTGEYMGRA"
     gene            complement(2813..>3517)
                     /locus_tag="PLS64_08105"
     CDS             complement(2813..>3517)
                     /locus_tag="PLS64_08105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRL43821.1"
                     /translation="KIEKIRCIKDESEIKKIAKAQEITEKSYLEVLNYLKPGVSERRV
                     ALELEHLIKLNGGEGVSFDLITITGKKTSLPHGVPSDDIVSEGDFFTFDIGSIFDGYH
                     SDTTRTVAIKSADEEMKKVYDIVLKAQLAVLDSVKPGAKCSDVDKIARDIISENGYGK
                     YFGHATGHGVGLDIHESPVVSPRSETMLKKGMIITDEPGIYLPGKFGVRIEDMLCVTD
                     TGYKNFVSLPKELIIV"
BASE COUNT         1031 a          775 c          593 g         1118 t
ORIGIN      
        1 gtgagtaccc gacgcaattg tgtcaataag ttccttagaa caatcctttg gatgcgatgt
       61 cataaagcga agccaataat ctccgtcaat tgaatcgacc tcacgcaaaa gctgtgaaaa
      121 gtttactttc tcggaaagac cttttccgta ggaatttaca ttctgaccga gaagggtaat
      181 gtctttataa cccgactcaa tcatattttt agcctcagaa acaataatat tcttttcacg
      241 actgcgttct ctgcctctta cataaggcac aacgcaatat gtacagaagt tgttgcaacc
      301 gtacataatc ggtaaccaac ccttgaaagt gccgtcacgc ttaacaggaa atccctcata
      361 aagttgatta tcgtcattac ctctttcaaa cactcttttg ccatcaagaa gagcgttata
      421 cataagctca ggaaaatggt gcaatgaatg agtaccaaac acaagtccta caaaaggaaa
      481 gctcttgtaa attttattgg caacatgctc ctgctccatc atacaaccgc acaaagcaat
      541 aagaatctgc ggatgcttac gctttagatt ttttaatgca cctacattgc cgaacactct
      601 gtcctcagca tgttctctta cggcacaggt attgaaaagt atgaaatccg cattgtccgg
      661 ttcatctgca aactcaaatc ccgattgttc aagcataccc ttaatttttt cactatcagc
      721 cacattctgc tggcagccgt atgttctgat atacgcaaca ggcttctctc ctcttttgcg
      781 gatatccata atttcagcta ttaaattcat atactccgat tgtttttcgg aaattttctg
      841 actgtcaggc aaagaaatgc ctcctaaccc attgtattaa ctgttagatt ataacacaag
      901 atatacggtt tttcaatgtt tttcacaggt ttttcaaaat ttacttctta taagtctgaa
      961 acagagaacc gtatccggac ggtatattac cgactattac ggattgtgca atttcaaaat
     1021 cggtagtaaa tgttattttg cctgaataat caagcgaggt tgtcattatt tttgaggtga
     1081 gcaaaagctt tatatggtgg attgtctgat ttattcccgc ttgttcaaat gtacttataa
     1141 cttccgagga aaagcttcct gtaagattaa aatttacggg aatgcaaggt ccgacatttg
     1201 agagtattgt aatattcgta aatgcaccga gaggtatttc aatttcattg tcatatacct
     1261 tagctatttc cttttggaca gctttcgtga tgtcggcttt aattctgttg attttgaatg
     1321 aatctgtttc aattgactgc acatttccgt ttttatcaca attaatcttt gcaatatcac
     1381 tgtaagaata attaagcttg tcaagtgtgt tattcactgc atcacagaca gaatttacag
     1441 acaatatttc agcctgaatt cttatgtact ccggtctgaa atctgacaaa tgcacctcgc
     1501 agaaaacagt aacaccgata atcagtaaaa ccacagaaag aagcattttc tttaaaagaa
     1561 caaattttct tttacacctt tttctgtaca acataaaaac acccctcgta tatactatac
     1621 gaagggtgtt tgttttgtta attattcttc tgtgtccgaa caaccgtcgc atgatgcaca
     1681 atcggcatca cactcgtcat cttcctcatt gctgaaaagc tctgaaaaat caaactcaag
     1741 gttctcaccg cagttaggac actcgatttc gccttcaaga aggacatctt cggaaacatt
     1801 aagcttctga ccgcatgaat cacaagtcac ctcgtaaaaa ggattctctt catcatcaaa
     1861 atcgtaatcg tcatcttcat cgtcataatc ttcaatgtca tagtcctcgt catcgtaaac
     1921 atcagactca acatcagcaa gatcctgatc aatttcgtca acctgagcgc ttaactcatc
     1981 gtaaagttct tccatgtcag atacttcaaa agccatatca tcaagaagat caacgattgc
     2041 attaagaacc tttacttcag gttttgactc atcaagctcc agaccgtcgc aaagaccgcg
     2101 aatgtaagaa attttttcag taagattcat aactgattct cccgcagaaa ttatgctctg
     2161 cccatatatt cgcctgttct tgtgtcgatc tgaataactt cgccctcatc aataaagaga
     2221 ggaactttaa tttcagcacc tgtttcaaga gttgcaggct ttgtagcgtt ggttgctgtg
     2281 tcacccttaa agccgggatc tgtctttgta accttaagtt caacaaagtt cggaggctca
     2341 atgccgaata cattgccctt gtaagaaaga accttgcaca ccatattttc cttaacaaac
     2401 ttaaagttgt cgccgagaac gctcttgtta atcggaatct gctcccatgt ttctgtatcc
     2461 atgaagtagt aaagatcgcc gtctgaatag ctgtattcca tatcctttct gtcaatgaaa
     2521 gctgtgggat acttatcgtt cgggttgaaa gttttttcaa ctactgcgcc tgtaataaca
     2581 tttctgattt tagttcttac aaatgcagca cccttaccgg gctttacatg ctggaattca
     2641 atgattgata caacctgacc gtccatttca aatgtaacgc cgtttctgaa atcacctgct
     2701 gatatcatta gtaatacctc ctaattatct ttcatacgga aatacggtaa tgtatgccgt
     2761 aatataacaa tttacattat acataacaat gacaaaaaat gcaagacttt tattacacaa
     2821 taatgagttc tttggggagt gaaacaaagt ttttgtaccc tgtatcagtc acgcaaagca
     2881 tatcttcaat gcgaacaccg aatttacccg gcagatatat tcccggttca tcggttatta
     2941 tcataccttt tttcagcatt gtttcacttc tcggagatac aacgggagat tcgtggatat
     3001 caagtcctac accgtgaccg gttgcatggc cgaaatattt tccgtaaccg ttttccgaga
     3061 ttatatctct tgcaatttta tcgacatcag aacattttgc tccgggtttt acgctgtcaa
     3121 gtacagcaag ctgagctttt aatacgatgt cataaacctt tttcatttct tcgtcagccg
     3181 acttgattgc taccgtacgc gtagtatcgc tgtgatagcc gtcaaaaatt gagccgatat
     3241 caaaagtaaa gaaatctccc tcactgacaa tatcatctga cggaactccg tgaggcaatg
     3301 aagtcttttt gcctgtaatc gtgatgagat caaatgaaac accttcaccg ccgttaagct
     3361 ttatgagatg ttcaagctca agtgcaaccc tgcgttcgct gacaccgggt tttaggtaat
     3421 tcaacacctc aagatatgac ttttcggtta tctcctgcgc tttagctatt tttttaattt
     3481 ctgattcatc cttgatacat ctgatttttt caatctt
//
LOCUS       DAOMVE010000161         3487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_7870_length_3487_cov_2.991550, whole genome shotgun sequence.
ACCESSION   DAOMVE010000161 DAOMVE010000000
VERSION     DAOMVE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7870_length_3487_cov_2.991550"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..1342
                     /gene="argC"
                     /locus_tag="PLS64_08110"
     CDS             305..1342
                     /gene="argC"
                     /locus_tag="PLS64_08110"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HRL43822.1"
                     /translation="MKIKAGIVGATGYAGAELVRLLTAHPEAEISAISSVSFEGDKLS
                     DVYPSYTFLNDMVCENQDAVVEKSDVIFAALPHGLSQELAAKCIDAGKAFIDLGADFR
                     LESEEEYTEWYGGTFLDKKLHEQAVYGLPEFFRDKIKGKKLVANPGCYTTCSPLAIAP
                     AIVNGLVSTKGIICDCKSGVTGAGRKPNQGNHYPELNEGFHAYKVASHRHTPEIEQTL
                     SKLSGEKVIITFVPHLLPVNRGILATVYADIKDGVTFEQIRKAYEDFYKDEFFVRLLD
                     DGKCADIHNIKYSNFCDVSIHHDKRANKLVACAAIDNMVKGAAGQAIQNMNIIFGLDE
                     KTGLVIVPPAF"
     gene            1355..2581
                     /gene="argJ"
                     /locus_tag="PLS64_08115"
     CDS             1355..2581
                     /gene="argJ"
                     /locus_tag="PLS64_08115"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HRL43823.1"
                     /translation="MEIKYIENGSVTSAKGFTANGICAGVKASNTTKKDLALIYCDKV
                     CNSAAIFTKNLVKSDTIYVTKKHLENGTAQAVVVNSGNANACNPDGYEKAEKMCSLAA
                     DALGVDENDVIVASTGVIGQPLPIEPIASGIEKLKGTLSKDGGISAAEAIMTTDTVKK
                     QWAVETVLDGKKVTIGGIAKGSGMIHINMGTTLSFVTTDAAISSEMLKSALIEAADVS
                     YNMVSVDGDTSTNDTLAIMASGYAGNKEITEKNDDYKTFTAVLTDAFKKVAKKLAKDG
                     EGATKLLICKVDGAKDDKSAKIISKSIICSSLVKAAMFGADANCGRILCAIGYSGAET
                     DVKRIDVSYKSAKGEILVCKDGNGLVFDEDKAKEILLEDEIEILVKLGDGNASAEAYG
                     CDLTYDYVKINGDYRT"
     gene            2634..3485
                     /gene="argB"
                     /locus_tag="PLS64_08120"
     CDS             2634..3485
                     /gene="argB"
                     /locus_tag="PLS64_08120"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRL43824.1"
                     /translation="MDVSKRAKVLIQAMPYIKKYSGETIVVKYGGNAMINEELKAAVM
                     SDLVLMQLVGVNVVLVHGGGPEINAMLKAVGKESKFINGMRVTDKETIDIVQMVLAGK
                     VNKSLVQLLGSHGGKAMGLCGLDGRMLMAEKLVTGADLGYVGEIKEVNPEPLNNALAS
                     GYIPIVSTVAGGYDGNVYNINADIAASQIAAKIGAKKLILMTDIRGLLRDVHDEDSLI
                     PVVNVSEVPMLKRDGIISGGMIPKIDCCVEAVRSGVNRAHIIDGRIEHSILLELFSDE
                     GIGTMFY"
BASE COUNT         1048 a          746 c          821 g          872 t
ORIGIN      
        1 acaagaaatc tgacggtgca atatgcacaa acgatttaat cagtgattcc ttaaaacata
       61 aaaaaataac cgcccgacgc tctactttcg gacggttatt atgccagaca aagtaggtca
      121 accgctttaa acggttttcc ctgtagcctt attatatagt atatgcaaat aaaagtcaat
      181 atgaattgac agagatataa agtacggcga gccgccgttc ccggttcatg caaaaagtga
      241 actgccgtat tcaacacata caggcaaaga ttgatataaa tttacaatag attaggtgat
      301 agcaatgaaa ataaaagcag gtattgtcgg tgcaaccggt tatgcaggtg cggagcttgt
      361 aagacttctt accgctcatc ccgaggctga aatttcggca atcagctctg tttcctttga
      421 gggtgacaag ctgagcgatg tttatccgtc ttatacattt ttgaacgata tggtttgtga
      481 aaatcaggac gccgttgttg aaaagagcga tgttatcttt gccgctcttc cacacggtct
      541 ttcacaggag cttgccgcaa agtgcattga tgcgggcaag gcatttattg acctcggcgc
      601 tgacttccgc cttgaaagcg aagaagaata caccgaatgg tacggcggaa cttttcttga
      661 caaaaaactt cacgaacagg cagtttacgg acttcctgaa tttttcagag ataaaatcaa
      721 gggcaaaaag cttgttgcga atccgggttg ttacacaacc tgttcaccgc tcgcaattgc
      781 tcctgcaatt gtaaacggac ttgtcagcac aaagggcatc atttgcgact gcaaaagcgg
      841 tgttacgggt gccggcagaa agccaaatca gggcaaccac tatcccgaac ttaacgaagg
      901 attccatgcc tataaggttg caagccaccg ccacactccc gaaattgagc agactctttc
      961 aaagctttcg ggcgaaaagg ttattattac ctttgttccg caccttctcc ccgtaaaccg
     1021 tggcattctt gcaacagttt atgccgacat caaggacggt gtaacctttg aacaaatcag
     1081 aaaggcttat gaggactttt acaaggacga attttttgta agacttcttg atgacggcaa
     1141 atgtgccgac attcacaata tcaaatattc taacttctgt gatgtttcaa ttcaccacga
     1201 taagagagca aacaagctcg ttgcctgtgc cgcaatcgac aacatggtca agggtgccgc
     1261 aggtcaggct attcagaata tgaacatcat cttcggactt gacgaaaaaa caggtttggt
     1321 aattgttccg cctgcatttt aagggtgaat atctatggaa atcaagtata ttgaaaacgg
     1381 ctcggtcact tccgcaaagg gctttacagc caacggaatt tgtgccggag tcaaagcatc
     1441 taacacaact aaaaaagacc ttgcgctcat ttactgcgac aaggtttgca acagtgcggc
     1501 tatctttaca aagaaccttg taaagtccga cactatatat gttactaaaa aacaccttga
     1561 aaacggtacg gctcaggctg ttgtcgtaaa ttcaggcaat gcaaatgcct gtaaccctga
     1621 cggctatgaa aaggctgaga aaatgtgcag tcttgccgca gacgctctcg gtgttgacga
     1681 aaacgatgta atcgttgcat caacaggtgt catcggtcag cctctcccga ttgaacccat
     1741 tgcaagcgga attgaaaagc tcaaaggcac gctttcaaag gacggcggaa tctctgccgc
     1801 agaggcaatt atgacaaccg acaccgtgaa aaagcagtgg gctgttgaaa cggttcttga
     1861 cggcaaaaag gttacaatcg gcggtattgc aaagggcagc ggtatgattc atattaacat
     1921 gggtacaact ctttcctttg taacaaccga tgcggcaatt tcttccgaaa tgctcaaatc
     1981 cgcacttatc gaagccgctg atgtcagcta taatatggta agcgttgacg gtgatacctc
     2041 aacaaacgat acccttgcaa ttatggcatc gggttatgca ggcaacaagg aaatcacaga
     2101 aaagaacgat gattacaaaa cctttacagc cgttcttacc gatgcattca aaaaggttgc
     2161 aaagaagctt gcaaaagacg gtgaaggtgc aacaaagctc cttatctgca aggttgacgg
     2221 tgcaaaagac gataagagcg caaagattat ttcaaagagc atcatctgtt caagtcttgt
     2281 aaaggcggca atgttcgggg ctgacgcaaa ctgcggaaga atcctctgcg caatcggcta
     2341 cagcggtgcc gaaacagatg ttaaaaggat tgatgtttca tataaatccg caaagggtga
     2401 aatccttgtt tgcaaggacg gcaacggtct tgtgtttgat gaggacaagg ctaaggaaat
     2461 tttacttgag gatgaaattg aaattctcgt aaaattaggt gacggcaatg catctgccga
     2521 ggcttacggt tgcgacctta cctatgatta tgtaaaaatc aacggtgact accgcacata
     2581 atatgcgatt tgattgtgtg agcggaaatc aactacataa aggattgaag aaaatggatg
     2641 tttcaaagag agccaaggta cttatacagg caatgcccta tatcaagaaa tattcgggcg
     2701 aaacaattgt tgtaaaatac ggcggaaacg ctatgataaa cgaagaactt aaagcggcag
     2761 ttatgagcga cctcgttctt atgcagcttg tcggagtaaa tgttgtgctt gttcacggcg
     2821 gcggacccga aatcaatgca atgctcaaag ccgttggcaa ggaaagcaag tttatcaacg
     2881 gtatgagagt gaccgacaag gaaactatcg acattgttca gatggtgctt gcaggcaagg
     2941 tcaacaagag ccttgttcag cttctcggca gtcacggcgg taaggctatg ggactttgcg
     3001 gtcttgacgg cagaatgctc atggctgaaa agcttgttac aggcgcagac ctcggctatg
     3061 tgggcgagat taaggaggtt aaccccgaac cgctcaacaa cgctcttgca agcggttata
     3121 ttccgattgt ttcaaccgtt gcaggcggtt atgacggcaa tgtatacaat attaacgcag
     3181 acattgcggc atcgcagatt gccgctaaaa tcggcgcaaa aaagctcatt cttatgacag
     3241 atatcagagg acttctccgt gatgttcacg atgaggattc actcatcccc gttgtcaatg
     3301 tcagcgaagt tcctatgctc aagcgtgacg gcatcatcag cggcggtatg attcccaaga
     3361 ttgattgctg tgttgaggca gtccgcagcg gtgtaaaccg tgcccatatc attgacggca
     3421 gaatcgaaca ttcaattctg ctcgagcttt tcagcgatga aggcatcggt acaatgtttt
     3481 attgatt
//
LOCUS       DAOMVE010000162         3423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_8053_length_3423_cov_3.468824, whole genome shotgun sequence.
ACCESSION   DAOMVE010000162 DAOMVE010000000
VERSION     DAOMVE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8053_length_3423_cov_3.468824"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..1242
                     /locus_tag="PLS64_08125"
     CDS             493..1242
                     /locus_tag="PLS64_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRL43825.1"
                     /translation="MKLDTVLAKRENKVVYKDGALAAKVFDECYPKSDILNEALNQAR
                     VEETGLNIPKIEEVTKIDGKWAIISTFIEGKTLAELMAEHPEKEDEYLNLFVDIQVKI
                     LSQKAPLLNKLKDKMKRKISETDLDATTRYELETRLNGMPNHTKVCHGDFNPSNVIID
                     ESGTPYVIDWSHATQGNGSADAARTYLLFRLNHQEELAEKYMKLYCTKTDTARQYVDR
                     WLPIVAASQSVKGKPEEREFLLKWASVCEYQ"
     gene            1740..3332
                     /locus_tag="PLS64_08130"
     CDS             1740..3332
                     /locus_tag="PLS64_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HRL43826.1"
                     /translation="MQRIKKITAIILSVLTLSSVCVFGSSFSAGAKGTGAGLAEWALN
                     AYNSGWSYVYGGSTPGAVDCSGLIYSYAGGERCGNPQLETATETGSVSAGIPNVHGLG
                     LWRPGHVGVYVGNGMEVDARGDEYGVCYEAIGGYNNWTYWFKLAAVSYVTNGWESFNG
                     NYYYYENGEYIVNTSRTIDGTTYYFDSQGRSSKTPSNTSSSSSSSSSSSGSSGSSSSS
                     GSSSSSSNTPSVYKNGSSGAEVKKIQQRLADLGYYDGAVDGYFGDATEEAYKAFQKAA
                     GLTVDGIAGDSRNTLYSENAPEAPKADTTTKTEDKPEEETTAPTEATTEPVTEAVQYQ
                     NGDENDEVTAIQQQLIKLGYFAMDATGYYGEYTAQAVANFQNQNSLDSTGIVDEKTYN
                     ALFSDNAVANPKTEVVSGEVSPAVSAAPIEMPSSTGASQFILSNEKNQNYSDTASSVA
                     VQSVKSADKALAKSNAASTPTLVAAVQRSANVGLWFVLVAVILGAIAFVFFLRDRKLS
                     RYSRYVARKKKRATKAELDARW"
BASE COUNT         1055 a          677 c          733 g          958 t
ORIGIN      
        1 aaactatcta cacgaattgg gaacggcggc tcgccgtgcg ggcgtccaat gaacgcccct
       61 acgggattgt gcgaacggtt gcaatgttat gttcgggcat aatatgtttt tttacattac
      121 aaactgtctt cccgacatta attttccatt ttccattttc aattttacat ttaattaaac
      181 cttcctcttg ggcgtaatgt gcttttaata ttacaaacca ttatgcacaa tgcacaaaag
      241 aatgttaaaa atttcacaaa tatttagtta aaagtttaac aaatttttat gcacttcgca
      301 taaaatatct tgctcttttt gtttgaaagg taaaaaatat tttatttgca tttgctaaat
      361 gcacaaagaa aattaaaatt attttgttat ttttacacta tactaacccc tgaaattgtt
      421 gtataatata accgaagcaa tgaaagattg cttgatgaat ttttaatatt ttaaagcgaa
      481 aggggcttta ttatgaaatt agatacagtt cttgcaaaaa gagagaacaa ggtagtttat
      541 aaagacggtg cgcttgcagc caaagttttt gacgagtgtt atccgaaatc agatattctt
      601 aacgaggctc ttaatcaggc aagagttgag gaaacaggac tcaatattcc gaaaattgaa
      661 gaggttacaa agattgacgg aaaatgggca attatctcca ccttcatcga gggcaaaact
      721 cttgcggagc ttatggcaga gcatcccgaa aaagaggacg aatacctaaa tctctttgtt
      781 gacattcagg ttaaaatcct cagccaaaaa gcaccgttgc ttaacaaact caaggataag
      841 atgaaacgca aaatcagcga aaccgacctt gacgcaacaa caagatatga gcttgaaacc
      901 cgtcttaacg gtatgccgaa ccacacaaag gtttgtcacg gcgatttcaa cccgagtaat
      961 gttattattg acgagtcggg tacaccgtat gttattgact ggtcacacgc aacacagggt
     1021 aacggttcag ccgatgccgc aagaacttac ctcttgttcc gtttgaatca tcaggaagag
     1081 cttgcagaaa agtatatgaa gctttactgc acaaagaccg acacagcccg tcaatatgtt
     1141 gaccgctggc ttccgattgt agccgcatca cagtctgtta agggcaaacc cgaagagaga
     1201 gagttccttc tcaaatgggc aagtgtttgc gaataccagt aatgccgtat aaatgtattt
     1261 ttaattcata ttttactcag caaaatttca ttttgaaatt ttctctcctc tgtttttttc
     1321 ataattgtat tacggaaacc gaataaaagc agctcccgct ccaaatgctt ggtgcgggag
     1381 ttgctttttt gatgcgaaat ttatgttgac ggtacgctgt aaatattaca aaaggttaca
     1441 aattttgtaa taattcgctt taatttggtg cttgaaatga agattttgtg agtgaaactg
     1501 catcaaattt tctgaattag caaaaaatac gggctttggg gttgattttt taaaagagtg
     1561 atgttataat ggttacaatg ttgttatcaa ggtgtaacct gatttgtaat ttactgtcag
     1621 atttggtaac aatttttcgg tcaaatcttc cgtacagatt aaaacggact gtactttgag
     1681 tcggtcgtta tttttctgta agagctttga ctctctgata aaaatggagg taattaacga
     1741 tgcagagaat aaaaaagatt actgcaatca ttctgagtgt tctcacactt tcaagtgtat
     1801 gcgtattcgg ttcttcattt tctgccggtg caaaaggcac gggtgcaggt cttgcagaat
     1861 gggcactcaa tgcctataac agcggatggt catatgttta cggcggttca acccccggag
     1921 cagttgactg ttcaggtctt atctattcat atgccggcgg cgaaagatgc ggtaatccac
     1981 agcttgaaac agctaccgaa acaggttcgg taagcgcagg cattcctaat gttcacggtc
     2041 ttggactttg gcgtcccggt catgtcggtg tctatgtcgg caacggtatg gaggttgacg
     2101 caagaggtga tgaatacggt gtttgctatg aggcaatcgg cggttataac aattggacat
     2161 actggttcaa gcttgccgca gtttcatatg taacaaacgg ttgggaaagc tttaacggca
     2221 actactacta ctacgaaaac ggcgagtaca ttgtaaatac ttcaagaaca attgacggta
     2281 ctacatatta ctttgactca cagggcagat cgagcaagac tccttcaaac acttcttcaa
     2341 gcagttcatc aagttcaagc tcatcaggct catcaggttc ttcaagttca tcaggcagct
     2401 catcatcttc aagcaacaca ccgagtgttt acaagaacgg ttcatcgggt gctgaggtaa
     2461 agaagattca gcaaagactt gctgacctcg gctattatga cggtgcagtt gacggttact
     2521 tcggtgacgc aacagaagag gcttataagg cattccagaa agccgcaggt cttacagttg
     2581 acggtattgc aggcgattcg agaaatacac tttattcgga gaatgctccc gaagctccaa
     2641 aagccgatac aacaacaaag actgaggaca agcccgaaga agaaacaact gctcccacag
     2701 aggcgacaac agagcctgta acagaggcag ttcagtatca gaacggtgac gaaaacgatg
     2761 aagtaactgc aattcagcag cagcttataa aactcggcta ctttgcaatg gatgcaacag
     2821 gctactacgg tgaatataca gcgcaggccg ttgcaaattt ccagaatcag aattcactcg
     2881 attcaacagg tattgttgat gagaaaacat acaacgcact gttcagcgac aacgcagttg
     2941 caaaccccaa aacagaggtt gtaagtggcg aggtttctcc ggcggtttca gccgctccga
     3001 ttgagatgcc ttcttcaaca ggtgcgtcgc agttcattct ttcaaatgaa aagaatcaga
     3061 actactcgga cacagcatca agcgttgccg ttcagtccgt aaaatctgcc gacaaggctc
     3121 ttgcaaagtc aaatgccgca agcacaccga ctcttgttgc cgcagttcag agatccgcaa
     3181 atgtaggtct ttggtttgtg cttgttgcag ttatcctcgg cgcaatcgct tttgtattct
     3241 tccttcgtga cagaaagctt tcaagatatt caagatatgt cgcaagaaag aaaaaaagag
     3301 ccaccaaagc cgaacttgac gcaagatggt aattgatata taatccgatt attaaacagt
     3361 cgattgcaaa gcagtcggct gttttttgtt aattgaaaat ggaaaattga aaatggaaaa
     3421 ttg
//
LOCUS       DAOMVE010000163         3423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_8054_length_3423_cov_2.849466, whole genome shotgun sequence.
ACCESSION   DAOMVE010000163 DAOMVE010000000
VERSION     DAOMVE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8054_length_3423_cov_2.849466"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="PLS64_08135"
     CDS             complement(<1..199)
                     /locus_tag="PLS64_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43827.1"
                     /translation="MQISLTSAFATTSVSFEMQNADMKPNRLFSVALSANSGNRLSAA
                     TFEFTYDADYIEFRKVKAVNNG"
     gene            271..363
                     /locus_tag="PLS64_08140"
     tRNA            271..363
                     /locus_tag="PLS64_08140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:306..308,aa:Ser,seq:gga)
     gene            664..1377
                     /gene="pyrH"
                     /locus_tag="PLS64_08145"
     CDS             664..1377
                     /gene="pyrH"
                     /locus_tag="PLS64_08145"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRL43828.1"
                     /translation="MKPKYKRVLLKLSGESLAGEMKHGIDFETVLKICKPIKECVDAG
                     VEVGIVVGGGNFWRGRSSGEMDRTRADHMGMLATTINALGVCDALEQLGVNVRVQTAI
                     AMHQIAEPYIRNKAVLHLQKGRVVVFGCGTGNPFFSTDTAAALRAAEIEADIVMKATM
                     VDGVYDSDPKKNPNAKKYDTISFHDVLNKDLAVMDSTAASLCKDNNLPILVFSIEDPE
                     NIYKAVCGENIGTIVTANN"
     gene            1431..1985
                     /gene="frr"
                     /locus_tag="PLS64_08150"
     CDS             1431..1985
                     /gene="frr"
                     /locus_tag="PLS64_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRL43829.1"
                     /translation="MQTQLKKAGESMDRRLEHMRTEFSEIRAGRANPAVLDKVKVDYY
                     GAPTPVNQLAAVSVTEARTLTIQPWDASVLRQIEKAIQTSDIGINPQNDGKIIRLIFP
                     PLTEDRRKEIVKDVQKIAEETKIQIRNVRRELIDKLKAMKKDGELTEDDLKQGEKKAQ
                     DLTDKYVKEVESVSAVKQKEILEM"
     gene            1987..2733
                     /locus_tag="PLS64_08155"
     CDS             1987..2733
                     /locus_tag="PLS64_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRL43830.1"
                     /translation="MAFFSKKKDEQIEVTDLNLPSHIGIIMDGNGRWAKKRGLPRKAG
                     HRQGAKTFRTITRYCSDIGIKYLTVYAFSTENWKRPQEEVDALMSLFKSYLNEALEDF
                     KDDSIVVKFIGDKSGFNDELRELMIENEESSKDRDGMVLNIAMNYGSRDEIVRAVRNI
                     SEDVRNGKISSDDITENLFSNYLYTAGQPDPDLIIRPSGEYRISNFLLWQSAYTEFVI
                     MNKLWPDFQKSDLDEALKIYSSRNRRYGGV"
     gene            2750..>3423
                     /locus_tag="PLS64_08160"
     CDS             2750..>3423
                     /locus_tag="PLS64_08160"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRL43831.1"
                     /translation="MKSLKPRLLTAVIGIPILLIVLFVSELWHPFVGIVIGAFSAFMI
                     GEYLHARKMLYKLHLSIPCMLFAFLLSVLVTTKYLYVLIFAFILTVFAVMIFKHEKTN
                     YADVAYSVFGTFLISFGMSAVPIACASSVSITFCYICIFALPWMADAGGFFIGASFGK
                     HKLCPKISPKKTVEGAIGGILFCDLTAVIIGLVFKFLVMPDANINFWALILLGVIDAP
                     VSILGD"
BASE COUNT         1054 a          651 c          730 g          988 t
ORIGIN      
        1 agccgttatt cactgcttta acctttctga attcgatata atcggcatca tatgtaaatt
       61 caaatgttgc ggcagaaagt cgattgccgg aatttgcgga aagtgcaaca ctgaacagtc
      121 tgttcggctt catatctgca ttctgcattt caaaacttac gcttgtggtt gcaaatgcac
      181 ttgttaaaga tatttgcaat aaagctacca aaattaaaat aatctcactt gatttttctg
      241 atttcatttg atataataaa taggtcgttt ggagagatgt ccgagctggc cgaaggagca
      301 tgattggaaa tcatgtatac ggtgaacacc gtatcagggg ttcgaatccc cttctctccg
      361 ccacaaacgc tgaactttat tttaggttca gcgttatttt tttgacctgt ttttcttaat
      421 aaaaggtgtt gtttttcata tatataattg tatcgtatgg caagatttgt tgtcaacgga
      481 tttacagtga agagttagca catacagtgc atttacggtc atttttaata attttttagc
      541 atttcatatg acataaccat tcagctttcg gttatttatt caaaaaactg ccactaaacc
      601 tttacaaacc ctacaagata ttgtattata ttagtaatta taaattacca tggggtgtta
      661 tgtatgaaac caaaatataa aagagtatta ttaaaacttt caggcgaatc acttgcaggc
      721 gaaatgaaac acggaattga ttttgaaact gttctcaaaa tctgcaagcc tattaaagaa
      781 tgtgttgacg caggcgtaga agtcggcatc gtagtaggcg gcggtaactt ctggcgtggc
      841 agaagctcag gcgaaatgga cagaacaaga gccgaccata tgggtatgct tgcaacaaca
      901 atcaatgcac tcggtgtttg tgatgcactc gaacagcttg gagttaatgt tcgtgttcag
      961 acagctatcg ctatgcatca gattgcagaa ccgtacatca gaaacaaagc agttcttcac
     1021 cttcagaagg gcagagtagt agtattcggt tgcggaacag gcaacccgtt cttcagcaca
     1081 gacacagcag cggctcttcg tgcggcagaa attgaggctg atatcgtaat gaaggctact
     1141 atggttgacg gtgtatatga cagcgatccc aagaaaaatc cgaatgcaaa gaagtacgat
     1201 acaatttctt tccatgatgt tctcaacaag gatctcgctg ttatggacag cacagcggca
     1261 tcgctttgca aggacaataa tcttccgatt cttgtattca gtattgaaga tcccgaaaat
     1321 atctacaaag ctgtatgcgg tgaaaacatc ggtacaatcg taacagccaa caattaattt
     1381 gaatttttta ctgacttttt aacaataatt attacacagg aggcataaat atgcagacac
     1441 agttaaaaaa agccggcgaa agtatggaca gacgacttga gcatatgcgc acggaatttt
     1501 cagaaatcag agcaggcaga gcaaatcccg cagttcttga taaggtaaag gttgactatt
     1561 acggcgcacc cacacccgta aaccagcttg ccgcagtatc tgttacagag gcaagaactc
     1621 ttaccattca gccatgggat gcgtctgtat taagacagat tgaaaaggct atccagactt
     1681 ccgatatcgg catcaaccca cagaatgacg gtaagattat tcgtcttatt ttcccgcctc
     1741 tcacagagga cagaagaaaa gaaatcgtaa aagatgttca gaagattgct gaggaaacaa
     1801 agattcagat tcgtaatgtc cgccgtgaac ttatcgacaa gcttaaagct atgaagaagg
     1861 acggcgagct tacagaggac gatctcaagc agggcgaaaa gaaagctcag gatcttacag
     1921 ataagtatgt caaagaagtt gagagcgttt ctgcagtaaa gcagaaagaa attttggaga
     1981 tgtaatatgg cttttttctc aaagaaaaaa gatgagcaga ttgaggttac agacctcaat
     2041 ctgccttcac atatcggaat tattatggac ggcaacggcc gttgggcaaa gaaaagagga
     2101 cttccgagaa aagcaggtca ccgtcagggt gcgaaaactt tcagaacaat aacaagatac
     2161 tgttccgata tcggaataaa gtaccttacc gtatatgctt tttcaaccga aaactggaaa
     2221 agaccgcagg aggaagttga tgcactaatg tcgctcttta aatcatatct taatgaagcg
     2281 cttgaagatt ttaaggatga cagtattgtg gtaaaattca tcggtgacaa gtcgggtttt
     2341 aacgatgaac ttcgtgagct tatgattgaa aacgaggaga gttcaaaaga ccgtgacggt
     2401 atggttctca atatagctat gaattacggc agtcgtgatg aaattgtcag agccgtcaga
     2461 aacatttccg aagatgtcag aaacggaaaa atttcttccg atgacataac agaaaatctt
     2521 ttttcaaatt atctttatac cgcaggtcag cccgatcccg accttattat cagaccgagc
     2581 ggtgaataca gaatttcaaa tttccttttg tggcagtcag cctacacgga atttgttata
     2641 atgaacaagc tttggcctga ttttcagaaa tcagaccttg acgaagcgtt aaaaatatac
     2701 tcctccagaa acagaagata cggtggagtc tgatgagagt tgatataaaa tgaaatccct
     2761 caagccacgg ctgctgacag ccgtaatagg aataccaata ctgctgatcg ttctgtttgt
     2821 ttcggaactt tggcacccat ttgtaggcat agtcatagga gcattctcgg catttatgat
     2881 aggcgagtat cttcatgcaa gaaagatgct ttataaatta cacctttcaa taccttgtat
     2941 gctgtttgct tttctgcttt cggttttagt tacgacaaag tatctgtatg ttttgatttt
     3001 tgcttttata ctgacagtct ttgccgtaat gatttttaaa cacgaaaaga caaactatgc
     3061 cgatgttgca tattcagtgt tcggaacttt tttaatttct tttggaatga gtgccgtacc
     3121 gattgcctgt gcatcatctg tatcaattac tttctgctat atatgtattt tcgcattacc
     3181 gtggatggca gacgcaggcg gcttttttat cggagcatcg ttcggcaagc acaaactttg
     3241 cccgaaaatc agccctaaaa agactgttga gggcgcaatc ggcggaatac ttttctgtga
     3301 cctcacagca gtaattatcg gacttgtgtt caaatttttg gttatgccgg acgctaacat
     3361 taatttctgg gcattgattc ttctcggtgt aattgacgct cctgtttcaa ttctcggtga
     3421 ttt
//
LOCUS       DAOMVE010000164         3309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_8457_length_3309_cov_2.325446, whole genome shotgun sequence.
ACCESSION   DAOMVE010000164 DAOMVE010000000
VERSION     DAOMVE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8457_length_3309_cov_2.325446"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1720
                     /locus_tag="PLS64_08165"
     CDS             <1..1720
                     /locus_tag="PLS64_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43832.1"
                     /translation="QHKFALSERGAKDLIQGIIACAVQNISFMFPVGLLYFFVNDLLN
                     GGIKPEKIWFYIVGCIIALALIFAVTYWQYNSTFLATYVETGTRRITLAEKLRKIPLS
                     FFGKKDLADLTSTIMADCTYLETAFSHFIPPFIGAMISTVVIAISLFFFDWRMAIASL
                     WVLPVAFAIIGLSSKVQKSLNRKSMDAKMVCADGIQECIETIQDLKANNAEQSYMAGL
                     DTKIDNVEKRSIINEFGTSTFVVSAGLVLKLGIATTALVGSILLVNGQLDVLTFFMFL
                     LVVSRLYDPMQSALQNLAAIISTNTNVARMNEILDHDVQTGNTRLTNKGYDVKFKNVE
                     FSYNNKDNVLKDVSFTAKQGEVTALIGPSGGGKTTVSRLAARFWDINKGKITVGGMDI
                     SKIEPETLLSLYSIVFQDVTLFNNTIMENIRLGKKDATDEEVLLAAKLANCDEFVSKL
                     PNGYNTDIGENGCQLSGGERQRVSIARAFLKDAPIILLDEATASLDVENETLIQTALS
                     KLIKNKTVIVIAHRMRTVAGADKIVVLSDGVVAEEGTAESLKKLNGIFVHMMNLQSES
                     SNWKLN"
     gene            1825..3252
                     /locus_tag="PLS64_08170"
     CDS             1825..3252
                     /locus_tag="PLS64_08170"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HRL43833.1"
                     /translation="MNVIDLNNIEKYRENNRIEAKKATGGLPKSLWETYSSFANTLGG
                     IILLGVEEYRDKSLHPVNLPDPDELIEEFWEIANDIQKVSTNILSYDDVYVQKIEEKH
                     IIVITVPKAKRFDRPVYLDGSIHNSYRRNGEGDYRCTLDQINAMVRDSEIKTQDMNII
                     ESMTASVFNTDSLHSYRIKMSTVKPNNRLQNLSDKDFLQAIGAIGVGENLLFHPTAAG
                     LLMFGRSEFIKKEYPSYCLEYKETTQDDKHTIISSNLNSDCENLYDFFIKVFEKISSD
                     IKLTANIKSDITPITTALQEALANCLINADYYGSNGVVIMKDDESITISNPGGFRVEL
                     DAARAGGISDPRNTAIMRMFNLIGIGSNDGFGISNIFSVWKSHELVLPVIEESFNPES
                     ITLKLSFVPESKSTKTQILQNRPDIETDRQDAVISYITDNITVTSKEISELLGISHKK
                     SKQLLHKLMAKDFVIIKNGKYTLKA"
BASE COUNT         1126 a          654 c          616 g          913 t
ORIGIN      
        1 tcagcataaa tttgctcttt cggaaagagg cgcaaaagac cttattcaag gtataatcgc
       61 ctgtgctgta cagaacattt catttatgtt tcctgtaggt ttactttact ttttcgtaaa
      121 tgatttgcta aacggcggca taaaacccga aaaaatatgg ttctacattg tgggatgtat
      181 catagcactt gctctgattt ttgcagtaac atattggcag tacaattcaa cctttcttgc
      241 aacatatgta gaaacaggca caagaagaat tacgcttgct gaaaagttaa ggaaaatccc
      301 tctgtcattc tttggcaaaa aagaccttgc cgacctcaca agcacaatta tggcagactg
      361 tacatacctt gaaacagcat tttctcattt catcccgccg tttatcggag ctatgatttc
      421 aaccgtagta atcgcaatca gccttttctt ttttgattgg agaatggcaa ttgcttctct
      481 ttgggttctt ccggttgcat ttgcgataat cggcttgtca tcaaaagttc agaagagttt
      541 aaaccgcaag tcaatggatg ctaaaatggt ttgtgcagac ggaattcagg aatgtatcga
      601 aacaattcag gacttaaagg caaataacgc cgaacagtca tatatggcag gccttgacac
      661 aaagatagac aatgtagaaa aacgctcaat aatcaacgaa ttcggcacat caacatttgt
      721 tgtatccgca ggacttgtcc tcaaactcgg cattgccaca acagcacttg tcggctcaat
      781 tttgctcgta aacggacagc ttgatgtact tacattcttt atgtttttgc ttgttgtatc
      841 aagactctat gatccaatgc agtctgcttt acagaacctt gccgcaatca tcagcacaaa
      901 cacgaatgtt gcgagaatga acgaaattct tgatcatgat gttcagacag gcaacacaag
      961 gcttacaaat aaaggctatg atgttaaatt taaaaatgta gaattctcat acaacaacaa
     1021 agataatgtt ctcaaggatg tttcgttcac agctaagcaa ggcgaagtta ccgcacttat
     1081 cggcccatca ggcggcggta aaactaccgt atcaagactt gccgcaagat tctgggatat
     1141 taacaagggc aaaatcaccg tgggcggtat ggatatttcc aagattgaac ccgaaacttt
     1201 gttatcactt tattcaatcg ttttccaaga tgtaaccctc ttcaacaata cgattatgga
     1261 aaacatcaga ctcggcaaaa aggacgctac agatgaggaa gttctccttg ccgcaaaact
     1321 tgcaaactgc gatgaatttg tttctaaact tcccaacggt tataataccg acattggtga
     1381 aaacggctgt cagctctcag gcggtgaaag acagcgtgtt tcaattgcac gagcatttct
     1441 taaagacgct ccgattatcc tccttgatga agctacagca tcacttgatg tagaaaacga
     1501 aactcttatt caaacagctc tttcaaaact tatcaaaaat aagaccgtaa ttgtaattgc
     1561 tcacagaatg agaacagttg caggtgcaga caaaattgta gttttgtcag acggtgttgt
     1621 tgcagaagaa ggtacagcag aaagccttaa aaaattgaac ggaatatttg ttcatatgat
     1681 gaacttacag tccgaaagca gtaattggaa actcaattaa atataaccac taaacgcaaa
     1741 aggaacaact ataacgtttg ttccttttct gcgttgacat attaaaaaca atactgtaat
     1801 attattttat aggataacgg agggatgaat gtgattgacc tcaacaacat tgaaaaatac
     1861 agagaaaaca atcgaataga ggccaaaaag gcaacaggcg gattacctaa aagcttgtgg
     1921 gaaacatatt catcctttgc aaatacactc ggaggaatta tacttttagg tgtagaagaa
     1981 tacagggaca aatcacttca tcccgtaaat cttcccgatc cggatgaact tattgaggaa
     2041 ttttgggaga tagcaaacga tattcaaaaa gtaagcacaa acattctttc atatgatgat
     2101 gtttatgtgc aaaaaattga agaaaagcat attatcgtta ttacagtgcc aaaagcaaaa
     2161 agatttgacc gtcccgtgta tcttgacggc agtattcata attcatacag aagaaacggc
     2221 gagggtgact accgttgcac actcgaccaa attaacgcta tggtgcgtga ttccgagatt
     2281 aaaacacagg atatgaatat catcgaaagc atgactgctt ccgtatttaa taccgattca
     2341 ctccatagct accgaataaa aatgtcaact gttaagccaa ataatcggct tcaaaatttg
     2401 tcggataaag atttcttgca ggcaatagga gcaatcggag taggggaaaa ccttttgttt
     2461 caccctacag ccgcaggact tcttatgttc ggtaggtcag aattcatcaa aaaagaatac
     2521 ccgtcctatt gtcttgaata taaggaaaca acacaagatg ataaacacac aattatttca
     2581 tcaaatttaa attctgactg tgaaaacctt tatgatttct ttataaaggt ctttgaaaaa
     2641 atttcatccg atataaaact gacagcaaac ataaaatcag acattacacc gattacaact
     2701 gccttacagg aagctcttgc aaactgcctt ataaatgccg actattatgg ttcaaacggt
     2761 gttgttatta tgaaagatga tgaaagcatt acaatatcca atccaggcgg atttagagtt
     2821 gaactcgacg ccgcaagagc gggcggtata tccgacccca gaaacacggc aattatgcgt
     2881 atgtttaatt tgataggaat cggcagtaac gacggcttcg gtatttctaa tattttctct
     2941 gtttggaaaa gtcacgaact tgttctgccg gtcattgaag aaagtttcaa ccccgaaagc
     3001 atcaccctaa agctttcgtt tgttcctgaa agcaaatcaa ctaaaactca aattttacaa
     3061 aatcgtcccg atattgaaac agacagacag gatgccgtaa tttcatacat tactgacaac
     3121 atcacggtaa cttcaaaaga aatttccgaa cttctcggaa taagtcataa aaaatcgaag
     3181 caacttcttc acaaacttat ggcaaaggat tttgttataa tcaaaaacgg aaaatacacc
     3241 ctaaaagcat aaaaggaact taatcaaatt tcggaacatc aatccgcgta agcgttgctt
     3301 ataattgct
//
LOCUS       DAOMVE010000165         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_8661_length_3253_cov_2.468418, whole genome shotgun sequence.
ACCESSION   DAOMVE010000165 DAOMVE010000000
VERSION     DAOMVE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8661_length_3253_cov_2.468418"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..504)
                     /gene="rpiB"
                     /locus_tag="PLS64_08175"
     CDS             complement(52..504)
                     /gene="rpiB"
                     /locus_tag="PLS64_08175"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRL43834.1"
                     /translation="MIALGCDHGGYNLICAIKKYLDEKGLEYKEYGTFSEESVDYPVY
                     AYKVATAITSGECSTGILCCGTGIGISIAANKVKGIRAAVVSNEFCAEMTRRHNNANI
                     LCMGGRVTSEEDAVKFTEIFLNTPEEGDRHLKRLAMIDEIEAGTFKVD"
     gene            complement(501..1211)
                     /gene="tsaB"
                     /locus_tag="PLS64_08180"
     CDS             complement(501..1211)
                     /gene="tsaB"
                     /locus_tag="PLS64_08180"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRL43835.1"
                     /translation="MKILSVDTSATAASVALSEEGKLIGETFINTSLTHSQTLIPMVE
                     QLLNNTKTEISDIDAIAVNAGPGSFTGVRIGVAAVKGLAFANNIPCVSVSTLESMAYN
                     FLSTDCIVCAVMDARCSQVYNALFEIKNGVVTRLCDDRALSLDDLKNELLSVDGRIII
                     AGDGTDITCKYIGNEIKNAESAPVNLKYQRASSTALVAFEMINNGQTVSAQELMPVYL
                     RLPQAQRELNKKLGGRTK"
     gene            complement(1208..1630)
                     /gene="tsaE"
                     /locus_tag="PLS64_08185"
     CDS             complement(1208..1630)
                     /gene="tsaE"
                     /locus_tag="PLS64_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRL43836.1"
                     /translation="MVKLISHSADETEQIGEKIAKKLHGTEVIALFGGLGMGKTAFTR
                     GLARALGVDDGVSSPTFALVNEYSGKYNIYHFDMYRVNSWDDLYSTGFFDYIDNGILV
                     IEWSENIEGALPENAIRITIEKGESDDERIFEIEGVEI"
     gene            complement(1632..>1925)
                     /locus_tag="PLS64_08190"
     CDS             complement(1632..>1925)
                     /locus_tag="PLS64_08190"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRL43837.1"
                     /translation="GVDFTEEAQVQIAKFNELGWDKLPICMAKTQYSLSDDPTKLGAP
                     KDFEITVRDVTISNGAGFAVIYTGNIMTMPGLPKVPAAEKINIDENGKISGLF"
     assembly_gap    1926..1935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1936..>3253)
                     /locus_tag="PLS64_08195"
     CDS             complement(<1936..>3253)
                     /locus_tag="PLS64_08195"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRL43838.1"
                     /translation="KYKAKLSDELADRVKDNPDGKLILVTAINPTPAGEGKTTTSAGL
                     GQAMAKIGKKAIIALREPSLGPVFGIKGGAAGGGYAQVLPMEDINLHFTGDMHAITAA
                     NNLCCAMLDNHIQQGNTLGIDPRRILFKRCLDMNDRALRNITIGLGGKVNGVPREDHF
                     IITVASEVMAILCLANDISDLKERLGNILVAYTYSGEPVYAKDLKAEGAMTALLKDAI
                     KPNLVQTLEGTPAIMHGGPFANIAHGCNSIRATKLALKLADYCITEAGFGSDLGAEKF
                     LDIKCRYAGIAPSAIVIVATCRALKYNGGVPKSEVSNENIEALKKGIVNLGVHIDNMR
                     KYNVPVVVAINQFGTDTDEELKYIEEYCISKGADFALSNVFGKGGEGGVELANKVVEA
                     CEKPSAFKPLYSLDLTLKEKVEKIAKEIYGASKVNYTTAAEKAIKEV"
BASE COUNT          816 a          747 c          656 g         1024 t
ORIGIN      
        1 gatgtcattc aacacctcta attcttcgtc ggacataaaa atctcccgaa atcagtcaac
       61 cttgaaagta cctgcctcaa tctcatcaat catagcaaga cgctttaagt gtctgtcgcc
      121 ctcttcggga gtattgagga aaatttctgt aaacttaaca gcatcttctt cgcttgtaac
      181 tctgccgccc atacaaagaa tatttgcgtt attgtgtctg cgagtcattt cagcacaaaa
      241 ttcgtttgaa acaactgcgg ctctgattcc ctttaccttg tttgcggcaa ttgatatacc
      301 gatgcctgta ccgcaacaga gaatgcctgt tgagcactca cccgaggtga ttgcagttgc
      361 taccttgtag gcataaaccg gataatcaac gctttcttcg ctgaaagtgc cgtactcttt
      421 gtattcaagt cctttttcat ccagatattt tttgattgcg cagataaggt tatatccgcc
      481 gtggtcgcat ccgagtgcta tcattttgtt ctacctccaa gttttttatt aagctcacgc
      541 tgtgcctgag gcaagcggag atataccggc ataagctctt gtgccgaaac ggtttgtccg
      601 ttattaatca tttcaaatgc aacaagtgca gttgaagacg ctctctgata tttaagattt
      661 acgggagcag actctgcatt tttaatttca ttaccgatgt atttacaggt aatatcggta
      721 ccgtcacctg ctatgatgat tcttccgtca acagacagca gttcgttttt taagtcatca
      781 agcgataatg ctctgtcatc acaaagtcgg gtaacaactc cgtttttaat ttcaaacagt
      841 gcattgtata cctgtgaaca gcgtgcgtcc attacggcac atacaataca gtcggttgaa
      901 agaaaattat aagccatact ctcaagtgtt gaaaccgaaa cacacggtat attatttgca
      961 aaagcaagac cttttactgc cgcaacaccg attcttactc ccgtgaatga acccggacct
     1021 gcgttgactg caattgcatc aatatctgat atttccgttt ttgtattgtt caaaagctgt
     1081 tcaaccatag ggatgagcgt ctgactgtgg gtaaggcttg tgtttatgaa agtttcaccg
     1141 atcagcttgc cctcttctga aagtgcaaca gatgcggctg ttgcagatgt gtcaacagac
     1201 agaattttca aatttcaaca ccttcaattt caaagattct ttcgtcatcg ctctcccctt
     1261 tttcaatcgt tattctgatt gcattttcgg gaagtgcgcc ttctatattt tcactccact
     1321 caattacgag aatgccgttg tcaatgtagt caaaaaatcc cgttgagtac aaatcatccc
     1381 atgaattaac tctgtacata tcaaaatgat agatattgta ctttccgctg tattcgttta
     1441 caagggcgaa agtagggctt gacacaccgt catcaacacc aagggctctt gccagtcctc
     1501 ttgtaaaggc ggttttgccc ataccgagtc cgccgaaaag ggctattacc tctgtgccgt
     1561 ggagcttttt tgcaattttt tcgccgattt gctcggtttc atcggcactg tgagaaatca
     1621 atttaaccat atcagaaaag tccgctgatt ttgccgtttt cgtcaatatt aattttttct
     1681 gcggcaggaa ctttcgggag tcccggcatt gtcataatat tgcctgtata aattactgca
     1741 aaacctgcac cgtttgaaat tgttacatct cttactgtga tttcaaaatc ctttggagca
     1801 ccgagctttg taggatcgtc tgaaagcgaa tactgtgtct tagccataca gattggtaat
     1861 ttatcccatc ctaattcatt gaacttcgca atttgtactt gtgcttcttc tgtaaaatca
     1921 acaccnnnnn nnnnnacttc cttgattgct ttttctgcgg ctgttgtgta gtttaccttt
     1981 gacgcaccgt agatttcctt tgcaatcttt tcaacttttt ccttgagtgt gaggtcgagt
     2041 gaatagagcg gtttaaatgc agacggcttt tcacaagcct ctactacctt atttgcaagc
     2101 tctacgccgc cttctccgcc ttttccgaat acatttgaaa gggcaaagtc tgcacccttt
     2161 gaaatgcaat attcttcaat gtatttgagt tcttcatctg tatctgtacc gaactgatta
     2221 atcgctacaa ctacgggaac attgtacttt ctcatattgt caatgtgaac accgaggttt
     2281 acaatgcctt ttttgagtgc ctcaatgttt tcatttgaaa cctctgactt tggaacgccg
     2341 ccgttgtatt ttaatgcacg gcatgttgcc acgattacaa ttgccgacgg agcaatgccg
     2401 gcatatctgc acttgatgtc aagaaacttc tcagcaccga gatccgagcc gaaaccagct
     2461 tctgtaatgc agtaatcggc aagctttaat gcaagctttg ttgctcttat gctgttacag
     2521 ccgtgagcga tatttgcaaa aggtccgccg tgcataattg caggagtacc ctcaagcgtc
     2581 tgaacaaggt taggtttgat tgcatccttg agaagtgcag tcattgctcc ctctgctttc
     2641 aaatctttgg cataaacggg ttcaccactg tatgtgtatg caacaagaat gttgccgagt
     2701 ctttctttaa ggtcggagat atcatttgca agacagagaa ttgccataac ttccgatgca
     2761 actgtaatga tgaagtgatc ttccctcgga acaccgttca ccttaccgcc aagaccgatt
     2821 gtgatatttc ttaatgcacg gtcattcata tcaagacaac gcttgaagag aattcttctc
     2881 ggatcaatgc cgagtgtgtt gccctgctga atgtggttgt caagcattgc gcaacaaagg
     2941 ttgttggctg ctgtaattgc gtgcatatca cctgtgaaat gaaggttgat gtcctccatc
     3001 ggaagaacct gtgcataacc gccgcctgcg gcaccgcctt taattccgaa tacaggacca
     3061 agcgacggtt cacggagtgc gatgattgcc tttttaccga ttttagccat tgcctgacca
     3121 agacctgcag aagtggtggt tttgccctcg cctgccggtg ttgggttgat tgctgttacg
     3181 aggatgagct ttccgtcagg attgtccttt acacggtctg caagttcatc agaaagtttt
     3241 gctttgtact ttc
//
LOCUS       DAOMVE010000166         3251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_8667_length_3251_cov_3.652065, whole genome shotgun sequence.
ACCESSION   DAOMVE010000166 DAOMVE010000000
VERSION     DAOMVE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8667_length_3251_cov_3.652065"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="PLS64_08200"
     CDS             <1..131
                     /locus_tag="PLS64_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HRL43839.1"
                     /translation="SLYDNKICTGDEVAQCIGCLADRMKKIGFEIVTDRGDYKADI"
     gene            148..1575
                     /gene="hydG"
                     /locus_tag="PLS64_08205"
     CDS             148..1575
                     /gene="hydG"
                     /locus_tag="PLS64_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HRL43840.1"
                     /translation="MNKYDKNSLKAEEFINDGEILDSLKFADENKNNLELVDKIIEKA
                     RLKKGINHREASVLLACEDKERIEEIYNLARQIKKDFYGDRIVMFAPLYLSNYCVNGC
                     VYCPYHAKNKHICRKKLTQDEVRKEVIALQDMGHKRLAIEAGEDPVNNPIEYILDCIK
                     TIYSVNHKNGAIRRVNVNIAATTVENYKKLKEAGIGTYILFQETYNKKSYEQLHPTGP
                     KHNYNYHTEAMDRAMQGGIDDVGIGVLFGLELYRYELAGLLMHAEHLEAVHGVGPHTI
                     SVPRIKAADDINPDDFDNGIDDETFAKIVAIIRIAVPYTGMIISTRESESVRKKVLEL
                     GISQISGGSRTSVGGYDEPESEEENSAQFDVSDNRSLDEVVRWLMNLGYIPSFCTACY
                     REGRTGDRFMSLCKSGQIQNCCHPNALMTLEEYLVDYASDDTRNVGQKLIEQELEKIP
                     NEKVRTIAKEHIFDIRNNNKRDFRF"
     gene            1587..2762
                     /gene="hydF"
                     /locus_tag="PLS64_08210"
     CDS             1587..2762
                     /gene="hydF"
                     /locus_tag="PLS64_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HRL43841.1"
                     /translation="MGMNDTPSSERLHIGFFGRRNAGKSSVVNAVTNQNMSLVSDKKG
                     TTTDPVEKTMELLPLGPVVIIDTAGFDDGGTLGTMRVERTKAVLNRVDIAVLVVDGTI
                     GMTSVENELVSLFEEKKIPYLIVFNKCDLLDNTTDGKIFVSAKNNTNIELLKDKIAKV
                     VNAQKSDKRLCGDLVNKNDFVVLVIPIDSAAPKGRIILPQQQVIRDLLDSGAIPVCVR
                     ESELADTLKNLGTKPKLVITDSQVFKPVSEIVPNDIKLTSFSILMARYKGFLKTAVNG
                     ARAIESLNDGDKILISEGCTHHRQCDDIGTVKLPRLLKNYTGKNFEIVTSSGKGFPNN
                     LSEFSLAIHCGACMLNSREVISRMNRSVESGVPFTNYGIAIAYMRGILDRSIEMFDE"
BASE COUNT         1050 a          667 c          729 g          805 t
ORIGIN      
        1 actcgctcta cgacaacaaa atctgtacgg gagacgaagt ggcgcagtgt atcggctgtc
       61 ttgcagacag aatgaaaaaa atcggctttg aaatcgtgac cgaccgagga gattacaaag
      121 cggatattta atccgaaagg aaatattatg aataaatacg ataaaaattc gctgaaagcg
      181 gaggagttta tcaatgacgg ggagattctt gattccctga aatttgccga tgaaaataaa
      241 aataaccttg aacttgtcga taaaataatc gaaaaggcaa ggctcaaaaa gggtatcaat
      301 caccgtgagg caagcgttct tttagcttgt gaagataaag agagaatcga agaaatctac
      361 aaccttgccc gtcaaattaa gaaggatttt tacggtgaca gaattgtcat gttcgcccct
      421 ctctatcttt caaactactg cgtaaacggc tgtgtctatt gtccgtatca tgcaaagaac
      481 aagcacatct gccgtaaaaa gctcacacag gatgaggtca gaaaagaagt tatcgcactt
      541 caggatatgg gacacaagcg tcttgcaatt gaggcaggcg aagatcccgt aaacaacccg
      601 attgagtaca ttctcgactg cataaagacc atttattcgg tcaatcacaa gaacggcgca
      661 atcagacggg ttaatgtgaa cattgccgca acaaccgttg aaaattataa aaagctcaag
      721 gaagccggaa tcggcacata cattcttttt caggaaacct acaataaaaa gagttatgaa
      781 cagctccatc ccacaggtcc caagcacaac tacaattacc acaccgaggc aatggacaga
      841 gccatgcagg gcggtattga tgatgtggga atcggtgtgc ttttcgggct tgagctctac
      901 cgctatgaac ttgcaggact tcttatgcac gcagaacatc ttgaggctgt tcacggtgtc
      961 ggtccgcaca caatcagcgt tccccgaatc aaggcggcag acgatataaa tcccgatgat
     1021 tttgacaacg gaattgacga tgaaaccttt gccaaaatag tcgcaattat cagaattgca
     1081 gttccctaca cgggtatgat tatttcaacc cgagagagcg aaagcgttcg caaaaaggtg
     1141 cttgaactcg gcatttctca gatcagcgga ggctcacgca caagcgtagg aggatatgac
     1201 gaacccgaat cggaagaaga aaattcggct cagtttgatg tttccgacaa ccgcagtctt
     1261 gatgaggttg taaggtggct tatgaatctc ggctatattc cgtcattctg caccgcctgt
     1321 taccgtgagg gcagaacggg cgaccgcttt atgtcgcttt gcaagagcgg tcagattcag
     1381 aactgttgcc accccaacgc ccttatgacg cttgaagaat atcttgttga ttatgcaagc
     1441 gatgatacaa ggaatgtcgg tcaaaaactc attgaacagg aactcgaaaa aatccccaac
     1501 gaaaaagtcc gcaccattgc aaaagaacat atctttgaca taagaaacaa caataaaaga
     1561 gatttcagat tttgacggag ggcgtaatgg gaatgaatga tacaccgtcg tcggaacgac
     1621 tgcacatcgg atttttcggc agacgaaatg caggaaaatc aagcgttgtg aatgcggtca
     1681 caaatcaaaa tatgtcgctt gtatcggaca aaaaaggcac aacaaccgat cctgtggaaa
     1741 agacaatgga gcttcttccg ctcggtcctg ttgtgataat cgacacggca ggctttgatg
     1801 acggaggaac tctcggcaca atgcgtgttg aacgcacaaa agccgtgctg aaccgtgttg
     1861 acattgccgt gcttgtggtt gacggaacaa tcggaatgac ctcggttgaa aacgaacttg
     1921 tatcgctttt tgaggaaaag aaaattccgt atcttattgt gttcaacaaa tgcgatttgc
     1981 ttgacaatac aactgacggt aaaatctttg taagtgcaaa aaacaacacg aatatcgaac
     2041 ttttaaagga taaaatcgca aaagttgtaa acgcacaaaa atccgataaa aggctttgcg
     2101 gtgaccttgt taataaaaat gactttgttg tgcttgtaat cccgattgac tccgccgccc
     2161 ccaagggcag aataattctt ccgcagcagc aggttatccg tgatttgctc gacagcggag
     2221 caatccctgt ttgtgtgcgt gaaagcgaac ttgccgatac tctgaaaaat ctcggtacaa
     2281 aaccaaagct cgtcataacc gacagtcagg tatttaagcc tgtttcggag attgtgccga
     2341 acgatatcaa actgacctcg ttttcaattc ttatggcgag gtacaaggga tttctgaaaa
     2401 ctgccgtaaa cggtgcaagg gcaattgagt cgcttaatga cggtgacaaa attctcatca
     2461 gcgagggttg cactcatcac aggcaatgcg atgacatcgg cacggtaaaa cttccccgat
     2521 tgctgaaaaa ttacacgggt aaaaattttg aaatcgtgac aagctcaggc aaaggcttcc
     2581 cgaataattt gtcggaattt tcacttgcaa tccattgcgg tgcgtgtatg ctcaattcaa
     2641 gagaggtaat cagccgaatg aaccgttcgg ttgaaagcgg agttccgttc acaaactacg
     2701 gaatcgccat agcctatatg cgtggaattc tcgaccgttc aattgaaatg tttgatgaat
     2761 aaaataaaaa ttcggtatgt aaaaatacac accgaaacag attgacatat taaaaaattg
     2821 atgttaatat ataagtaagg aaacggcttt agctgtttcg caattgggat ttattatttc
     2881 aaaatcgctc tgtattgcag tacagggcgg ttatttcttt atgcataaaa ctataatagt
     2941 tactaacaaa gtaaaacaga ttatgtattc cacgcaaaca ccccctttca taagggtagt
     3001 cttgcgaccg ccgccagtgg cggaaaaagg gagcatagcg ctgtctgaaa taacgagtat
     3061 aaggaagtgc atttatgcac aacgagatac gactatattt cagactggac agccgccgcc
     3121 atttccttac agagttattt taacaaatat attgtttgtt ttcaagacgg aagtttgtgc
     3181 tttgtaaaat aaaaattcgg tatgtaaaaa tacacaccga aacagattga cataattaaa
     3241 aattgatgtt a
//
LOCUS       DAOMVE010000167         3197 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_8890_length_3197_cov_3.119351, whole genome shotgun sequence.
ACCESSION   DAOMVE010000167 DAOMVE010000000
VERSION     DAOMVE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8890_length_3197_cov_3.119351"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLS64_08215"
     CDS             complement(<1..874)
                     /locus_tag="PLS64_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43842.1"
                     /translation="MKKKVIIIIAVVCVAIIAVVGTIFGVNAYNNYTIQQQTEQQVKS
                     IDDTYSKFTKETDRAKKLVILSDFIKNKPSTSDEIKVEVLNSVEPKYNETLAKMQKFF
                     TDDYDKTIKDNTIDSKTLKKTNDKKKLQSCIDNLEALKKTIDSEKSNVFYKKDIGNYD
                     KKPDELISSYNDRITAIEKAEAEAKAKKEAEAKKKAEEKAKQEKKKQTESSNTNNTDN
                     SYSDNTNSYSDSGNSYDNGDNNYSSNDSTYSSGNNYNYDSGNSSSSSSEKHYDSWYED
                     ENGKSYYDESTGEAW"
     gene            complement(1016..2692)
                     /gene="leuA"
                     /locus_tag="PLS64_08220"
     CDS             complement(1016..2692)
                     /gene="leuA"
                     /locus_tag="PLS64_08220"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRL43843.1"
                     /translation="MKPSFEKYIPFKQINLPDRQWPNNTITKAPIWCSVDLRDGNQAL
                     VDPMNLEEKLEFFHALCDMGFKEIEIGFPSASETEYEICRELIENNHIPDDVTIQVLV
                     QAREHLIRKTFEAVKGAKNVIVHFYNSTSTLQRKVVFKTDMDGVIKIAVDGAKLIKQL
                     TDEYDGDTNFRFEYSPESFSGTEPENAVEICDRVIEALGSTAENKLIVNLPNTVEMCT
                     PNTYADQIEYFARNLKHRDAAIISIHPHNDRGCGVAAGELALLAGAERVEGTLFGNGE
                     RTGNMDIVTMGLNMFTQGVDPKLDFSNLPKLREIYERCTNMKIDPRQPYIGELVFTAF
                     SGSHQDAINKGMKYMKESGTDYWEIPYLPIDPADVGREYEPIIRINSQSGKGGAAFVM
                     NNNFGYDLPKRMHPEFSKLVQAKCEELGRELIPKELFEVFEKNYLEHKQKYVLSSRKI
                     YEESENGNDYVHFKGKMKINGNEEVSLSGVGNGPIDAFFNAIKKVGLDKYEFISYSQH
                     AISQGSDSKAVSYIELKKPDGKNIFGIGIDSNVNVASVLGVLNAINRAEA"
     misc_binding    complement(2738..2986)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          858 a          664 c          588 g         1087 t
ORIGIN      
        1 cccatgcttc tccagtgctt tcatcgtaat aacttttgcc attttcatct tcataccagc
       61 tatcataatg cttctcggat gagcttgatg atgaattgcc tgaatcatag ttataattat
      121 ttccactgct gtaggtacta tcgttagaac tgtaattatt gtcaccgttg tcgtagctgt
      181 ttccgctgtc gctgtaactg tttgtattat cactgtagga gttatcggtg ttgtttgtgt
      241 tactgctctc ggtttgcttc tttttttcct gtttagcttt ttcttcggct ttcttttttg
      301 cctcggcttc ttttttagcc tttgcttcag cttcggcttt ttcaatagca gttattctgt
      361 cattatacga actaatcaat tcgtcaggct ttttatcgta gttgccaata tcctttttat
      421 agaatacatt gcttttttcg gaatcaattg ttttctttaa agcctcaaga ttatcaatac
      481 aagactgcaa cttcttttta tcgttagttt ttttaagagt tttgctgtca atagtgttat
      541 cttttattgt cttatcataa tcatctgtga aaaacttttg catttttgca agtgtttcat
      601 tgtactttgg ctcaacagag tttaacactt caaccttgat ttcatcactt gttgatggct
      661 tgttcttaat gaaatcggat aatataacta actttttggc tctgtcggtt tcctttgtaa
      721 attttgagta tgtatcgtca atacttttga cttgctgttc tgtctgttgc tgtatggtgt
      781 aattgttgta agcatttacg ccgaagattg taccgacaac ggcaataatc gctacacaaa
      841 ccactgcaat aattataatt actttctttt tcattttaat gcctaccatt tctatttgcg
      901 attatatgtt tacattatat atgtctgcgt ttcaaaagtc aattattaac atcttgtttc
      961 ataaaaaaca ggactgcaca ttcgcacagc cctgaaaatt gaattgttaa attgattaag
     1021 cctcggctct gttgattgcg ttgagaacac cgagtaccga tgcaacattg acatttgagt
     1081 caatgccgat gccgaagata ttctttccgt caggcttttt gagttcgata tatgaaacag
     1141 cctttgagtc ggaaccctga gaaattgcgt gctgactgta tgagatgaac tcgtacttgt
     1201 caagaccgac ctttttgatt gcattgaaga atgcgtcaat aggaccgttg ccgacaccgc
     1261 tgagagaaac ctcttcgttg ccgttaatct tcatcttgcc cttgaagtga acataatcat
     1321 tgccgttttc gctctcttca taaatctttc tgcttgagag aacatatttc tgcttatgct
     1381 caagataatt cttctcgaac acctcaaaca gctctttcgg aataagctct ctgccaagct
     1441 cctcacattt tgcctgaaca agctttgaaa attcgggatg cattctcttc ggaaggtcat
     1501 aaccgaagtt attgttcata acaaaagccg caccgccctt accggactgt gagttgattc
     1561 tgataatcgg ctcatactct ctgccgacat cggcaggatc aatcggaaga tacggaattt
     1621 cccagtaatc agttccgctt tccttcatat atttcatacc cttgttgata gcgtcctgat
     1681 gtgagcccga gaatgcagta aatacaagct cgccgatata tggctgacga ggatcaatct
     1741 tcatatttgt gcaacgctcg tagatttcac ggagctttgg aagatttgaa aagtcaagct
     1801 taggatcaac gccctgtgtg aacatattaa gtcccattgt aacgatatcc atattacctg
     1861 tacgctcacc gttgccgaag agtgtaccct caactctttc tgcacctgca agcaatgcaa
     1921 gttcgcctgc ggcaacaccg catccacggt cattgtgtgg gtgaatactg atgatagcgg
     1981 cgtcacggtg tttaagattt cttgcaaagt attcaatctg atctgcatat gtgttcggtg
     2041 tgcacatctc aacagtattc ggaagattga caatcaactt gttttcggca gtcgaaccga
     2101 gtgcctcgat aaccctgtcg cagatttcaa cagcgttttc aggctcggta ccgctgaagc
     2161 tttcgggaga atattcaaat ctgaagtttg tatctccgtc atattcatcc gtaagctgtt
     2221 taataagctt tgcaccgtca acggcaattt tgattacgcc gtccatatct gttttgaaaa
     2281 caacttttct ttggagagtt gaagttgagt tatagaaatg aacgataaca ttcttagcac
     2341 ccttaacagc ctcaaaagtt tttctgataa gatgctctct tgcctgaaca agaacctgaa
     2401 ttgttacatc atcgggaatg tggttgtttt caataagctc acggcagatt tcatattctg
     2461 tttctgacgc actcggaaaa ccgatttcga tttccttaaa gcccatatcg caaagtgcgt
     2521 ggaaaaattc gagcttttct tcaaggttca taggatcgac aagcgcctga ttaccgtcac
     2581 gaaggtcaac actgcaccag atcggtgctt ttgttatagt attgtttggc cactgtctgt
     2641 ccggcagatt aatctgctta aagggaatgt acttttcaaa tgatggtttc atataataac
     2701 ctcctgttaa atagcacaaa aaaatcgccc ccctaaacaa agggacgaat aaaatccgtg
     2761 gtaccacccg tattcaagaa cacatcactg tgttctcctt aaagacttct gacaaagtcc
     2821 cgaccgataa cgcagtcttg cgtttcgcat tacatatata tagtatatac acttcacacg
     2881 aaaaactcgg ggatgagcta tccatacaat gttccatgct gactcacacc aaccgtcagc
     2941 tctctttgcc ggttcatttg catcttgttc ccgtcaatgt ttttaagggg attcatttgt
     3001 taatactatt atatatatat acccattctt tgtcaagaaa attgttttga aaatctgtcg
     3061 aataaagttt atttttaaaa aaagggtgta tatagtgaat tttgggataa aataaacaac
     3121 aaatgagttt aaaatgtaga aattgccgat aatgtaaaaa agataaaaaa attgtcaaaa
     3181 aggggtttac aaaagag
//
LOCUS       DAOMVE010000168         3176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_8981_length_3176_cov_3.334829, whole genome shotgun sequence.
ACCESSION   DAOMVE010000168 DAOMVE010000000
VERSION     DAOMVE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8981_length_3176_cov_3.334829"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLS64_08225"
     CDS             complement(<1..259)
                     /locus_tag="PLS64_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43844.1"
                     /translation="MKKIFLLLVTALFLLSLYGCNTSSSSNNSTEQTTVTNTDDLSLD
                     TDTDSETEFNYDLEIYYNDKKIDNLNLFDAFMPTEDGLIYSK"
     gene            complement(458..2554)
                     /locus_tag="PLS64_08230"
     CDS             complement(458..2554)
                     /locus_tag="PLS64_08230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRL43845.1"
                     /translation="MRYKYQLDNLNCAHCAGKIEAKIAETDGFEDVSFNFATKLLNFK
                     SEKQNPVSEIQAICDSIEDGVTVVDKTPKNDITTKAEPEITKKKINRDTIFLAISIVL
                     AVVSEILHLTLDGVTAVHYVVLAACFVATLLSGYKVFIKGAKNILKLRIDETTLMAVA
                     VIAAFCLGDFVEAAMVTILFSIGEIFEDRAVDASRRDIEKLANIRPDTATIIVDGAEQ
                     IVPAESVEIGSIIEVKPYERVPLDGIIIKGKTTLDTSALTGESLPVDAGEGSEVLSGA
                     INGSNLITVKTTKHFGDSTATRILQLVEDAAAQKGNSEKLISRFASVYTPVVVLIAVA
                     IAVIPPLCGLGAFSEWLYRALVCLVASCPCAIVISVPLSYYSGIGAASEVGVLIKGGK
                     YIEALAKADTFVFDKTGTLTSGKLSVNKVNTVGSYSADEVLALAAASEKYSAHPIASA
                     IREKANGLELPELSDYSESAGHGTSAVYNGKTIQCGGSKILSDEQKKIANKDDSVFVI
                     YDGQLIGSLNVSDTVRPEAKEVISQLKALGVKNTVMLTGDRQQPAENVKNELGLSECH
                     AELLPAQKLELFKGLKSKSKGACFVGDGINDAPVLSASDCGIAMGFGSEAAIEASDAV
                     LASGTLKQLPKAIKIARSVINTVKGNIIFALSIKALVIILAAFGLAQIWMSVIADTGV
                     SVVCVLIAARLLKKKY"
     gene            complement(2567..2884)
                     /locus_tag="PLS64_08235"
     CDS             complement(2567..2884)
                     /locus_tag="PLS64_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRL43846.1"
                     /translation="MENKEKTTTPEMPDIDVLFELADLFKVFGDSTRLRIMYTISENE
                     MSVLNIAEALSMEQSTISHQLRVLRQNKLVRVRREGKQIYYSLDDDHVKRIIEMGLDH
                     VLE"
BASE COUNT          890 a          642 c          671 g          973 t
ORIGIN      
        1 tttttgaata tatgagtcca tcctcagttg gcataaatgc atcgaaaaga tttaaattat
       61 caatcttttt atcgttatag taaatctcca aatcgtaatt aaattctgtt tcagaatctg
      121 tatctgtatc aagagataaa tcatctgtat tcgttacggt tgtttgttca gtggaattat
      181 tgcttgatga agaagtgtta catccatata atgaaagtaa aaataaagcg gttactaata
      241 ataaaaatat ctttttcaaa ttaatctctc cgaagtttat ttatcagtta caagcgctga
      301 caaaaacatt ataatttata taattataaa agtcaatgat tttaaaatac tataatataa
      361 gattatgttt agaaaaattt tggcaaatga aagcaccgtg aaacttgtga aagctcacgg
      421 tgctttatca taataaatga tatgatttgc aaaaaaatca atatttcttt ttgaggagtc
      481 ttgcggctat gaggacgcag actacgctga cgcctgtgtc ggcgataacg gacatccaga
      541 tttgtgcaag accgaatgcg gcgaggataa ttacaagagc cttgattgag agggcaaaga
      601 tgatgttgcc cttgacggtg ttgataacac ttcttgcaat tttaattgcc tttggaagct
      661 gtttgagtgt gcctgatgcg agaactgcgt ctgacgcttc aatagcggct tcactgccga
      721 atcccattgc gataccgcag tcgcttgccg aaagaacagg ggcatcattg atgccgtcgc
      781 ctacaaagca agcacccttg gattttgatt tcaagccctt gaaaagttca agcttttgag
      841 cagggagaag ctctgcgtga cattcggaaa gtccgagttc atttttaaca ttttcggcag
      901 gctgttgtct gtcgcctgtg agcattaccg tgttcttgac accgagcgct ttaagctgtg
      961 agataacctc ttttgcctca ggtcttaccg tatcgctgac attgagcgaa ccgataagct
     1021 gaccgtcata aattacgaaa acagaatcgt ctttatttgc aatcttcttc tgttcgtccg
     1081 aaagaatctt tgaaccgccg cactgaatag ttttgccgtt ataaactgcg cttgttccgt
     1141 gaccggcact ttcgctgtag tcggaaagct cgggaagttc aagaccgttt gccttttctc
     1201 tgattgcgga ggcaatcgga tgagcggaat atttttcgct tgcagcggca agagcaagaa
     1261 cttcgtcagc actgtatgaa ccgacagtat taaccttgtt tacgctgagc ttgccgcttg
     1321 tgagcgtgcc tgttttatcg aatacgaatg tgtctgcttt tgcaagagcc tcaatatatt
     1381 ttccgccctt gatgagtaca ccgacttcgg atgccgcacc gatacccgaa tagtaggaga
     1441 gaggtacgga aataacaata gcacacggac atgacgctac aagacaaaca agcgcacggt
     1501 agagccattc gctgaatgct ccgagtccgc aaagcggagg aattactgcg attgcaactg
     1561 caatgagtac aacaaccggt gtgtaaacag aggcaaatct tgaaataagc ttttcgctgt
     1621 tgcccttttg agcggcggca tcttcaacaa gctgaagaat tcttgttgcg gtactgtcgc
     1681 cgaaatgttt tgtggttttg acggtaataa ggttactgcc gttgattgcg ccgctgagta
     1741 cctcactgcc ctcgcctgca tcaacaggaa ggctttcgcc tgtgagagcc gatgtgtcga
     1801 gagtcgtttt acctttaata ataatgccgt caagcggaac tctttcgtat ggcttgactt
     1861 caatgatact tccgatttca acgctttcgg caggaacaat ctgttccgca ccgtcaacaa
     1921 taattgttgc tgtatcggga cgaatgtttg caagcttttc aatgtcacgg cgggatgcgt
     1981 caactgctcg atcctcaaaa atttcaccga tagaaaagag gattgttacc attgccgcct
     2041 caacaaaatc tccaagacag aatgccgcaa ttaccgcaac agccataagc gttgtttcgt
     2101 caattctcag ctttagaatg ttttttgcac ccttgatgaa taccttgtaa cccgaaagaa
     2161 gtgttgcaac aaaacacgct gcgagtacaa cataatgaac agccgtaaca ccgtcaagtg
     2221 taaggtggag aatttcggac actacagcaa gtacaattga aattgcaagg aagattgtgt
     2281 cacggttgat tttctttttt gtgatttcgg gttctgcctt tgttgtgata tcatttttcg
     2341 gagttttgtc aacaacagtt acaccgtcct caatgctgtc gcagattgcc tgaatttcgg
     2401 aaacaggatt ctgcttttcg cttttgaagt tgagaagttt tgtcgcaaaa ttgaagctta
     2461 catcctcaaa gccgtctgtt tctgcaattt tagcctcaat cttgcctgca caatgagcgc
     2521 agttgagatt gtcaagctga tatttgtacc tcatatgtct ccctccttat tcaagaacat
     2581 ggtcaagtcc catttctatg attcgtttca catggtcatc gtcaaggctg taataaatct
     2641 gctttccttc acggcgaaca cgcaccagct tgttctgcct taacacacgc agttggtggg
     2701 aaattgtgct ttgctccatg ctgagagctt cagcaatgtt gagcaccgac atttcatttt
     2761 ccgaaatggt gtacataatg cgaagtcggg ttgaatcacc gaaaacctta aaaaggtctg
     2821 caagctcaaa cagcacatca atgtcgggca tttcgggagt ggttgttttt tctttgtttt
     2881 ccataggcgt cccctttaat tttctctcag gttaaaagtt atacttgaac atatgaagat
     2941 atgctcatat gttaattata atatagcatt ccctttttaa aaagtcaata gacacacacg
     3001 aaaaaatgta atgtttttca agtttttttg ccccaaagtt tcacttgttt attgtgtccg
     3061 tttggaaagt atatatagta tagaaagtta attttaaatt tgaaaaaaga agattgaagg
     3121 tgtaaaatta taataggaat aacggaacga tatttttctg tgttcaattt gaattg
//
LOCUS       DAOMVE010000169         3145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9106_length_3145_cov_4.029450, whole genome shotgun sequence.
ACCESSION   DAOMVE010000169 DAOMVE010000000
VERSION     DAOMVE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9106_length_3145_cov_4.029450"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..2373)
                     /locus_tag="PLS64_08240"
     CDS             complement(7..2373)
                     /locus_tag="PLS64_08240"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016148404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HRL43847.1"
                     /translation="MGNIMEKLELTAQSMYCKKIEELTPAELHYSLGKAVMGEIAGNW
                     EASKIKHDSERRAYYFSAEFLMGRMMYNNLYCLGILEDVTKLLKKKGIDINVFEDIDD
                     AALGNGGLGRLAACFLDSAATQEVPLDGYGIRYKYGLFKQLIENGYQVETADNWQRFE
                     DPWCNRVEDEAVTVSFKGQTVKAVPYDMAVIGCKTTNINTLRLWQAEAVNDFDFTAFN
                     NTEYNNAVQEKNDAENITKVLYPNDNKYEGKVLRLKQQYFFCSASLQDIMRRYKKKHG
                     NDFSFFAKENTIQLNDTHPVSCVPELVRLLMNEGFNFDDAFEIARKTFNYTNHTVLGE
                     ALEKWPADLMTQVIPEIYHIICLIANKCQEELTAKGVHEDTKAKMRIIDGNVVHMARL
                     ATYASTYVNGVAELHTEILKADVLKEWYQVYPERFQNKTNGITQRRWLGLCNPELSAL
                     ITEKVGSDAWLTDLSLLEKLNDCIDTRTITKFNNIKKKKKQQLADYIKKMDGYDVNPD
                     SIYDIQVKRLHEYKRQLLNAFSIMTIYFRLKDKKLKNWTPTTFIFGAKAAPGYARAKA
                     IIKYINEIAKLVNNDPETKDLLQVYFISNYNVSYAEKIVVAADLSEQTSTAGLEASGT
                     GNMKFMLNGAPTLGTLDGANVEIAECAGIENEYIFGAKVEDIERMKKEGYHPKALYDA
                     NPEIKRVVDTLIDGTFDDGGAQGEGSFKELHDSLLKDSSWQKADNYFLIYDLPDYVDT
                     KIRANTEYANRKEFGKKCLINIANAGKFSSDRTILQYAKEIWHTSYKK"
     gene            complement(2378..>3145)
                     /locus_tag="PLS64_08245"
     CDS             complement(2378..>3145)
                     /locus_tag="PLS64_08245"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRL43848.1"
                     /translation="IAVAGCPPDYFSATGQLWGNPLYDWDYLEATDYEWWFERIKAAS
                     KLYDITRIDHFRAFASYYSIPYPAENAINGEWVEGPRIKFFNMMEEALGKIDIVAEDL
                     GTLTPDVTELMEQTGYPGMKVLEFAFDSGEENDYLPHKYTENCVVYTGTHDNDTVMGW
                     LETAKPEDVNYARSYCQMPDDEPFNWGLIRVAYESKADTAIVPMQDILGLGKEARMNI
                     PSTLGGNWVWRLDGAALTDELADKLKTMSEKSGRLED"
BASE COUNT          790 a          711 c          694 g          950 t
ORIGIN      
        1 aacaaattac ttcttgtaag atgtgtgcca gatttcctta gcatactgga ggattgtacg
       61 gtcagatgag aacttacctg catttgcaat gttgataagg cacttcttac cgaattcctt
      121 acggttagca tactctgtgt tagctctgat ctttgtatca acataatcag gaaggtcata
      181 gatgaggaag tagttatcag ccttctgcca tgaagaatcc ttgaggagtg aatcatggag
      241 ttccttgaag ctgccttcgc cctgagcgcc gccgtcatca aatgtgccgt cgataagagt
      301 atcaacaact ctcttaattt ccggattagc gtcataaaga gcctttggat ggtagccttc
      361 cttcttcatt ctctcgatat cctcaacctt tgcgccgaag atgtactcgt tctcaatgcc
      421 tgcgcactca gcgatttcaa cattagcgcc gtcaagtgta ccgagtgtag gagcaccgtt
      481 gagcatgaac ttcatgttac ctgtacccga tgcctcaaga cctgctgttg atgtctgctc
      541 agaaaggtca gcagcaacaa caatcttctc agcgtaagat acattgtagt tagaaatgaa
      601 gtaaacctga aggagatcct ttgtttcggg atcgttgttt acaagcttag caatctcgtt
      661 gatgtactta atgattgcct ttgctcttgc ataacccgga gcagcctttg cgccgaagat
      721 aaatgttgta ggagtccagt ttttaagctt cttatccttc aatctgaagt agattgtcat
      781 gattgagaaa gcgttgagga gctgtctctt gtactcgtga agtctcttaa cctgaatatc
      841 atagattgaa tcgggattta catcgtagcc gtccatcttc ttgatgtagt ctgcgagctg
      901 ctgtttcttc ttcttcttga tattgttgaa ctttgtgatt gttcttgtgt caatgcagtc
      961 attgagcttt tcaagaagtg aaaggtctgt aagccatgca tctgagccaa ccttctctgt
     1021 gataagagca gaaagctcag ggttgcaaag accgagccaa cggcgctgtg taataccgtt
     1081 tgtcttgttc tggaatctct cgggatatac ctgataccac tctttgagta catcagcctt
     1141 gaggatttct gtgtgaagct cggcaacacc gtttacatat gttgatgcgt atgttgcaag
     1201 tcttgccata tgaacaacat taccgtcgat aatgcgcatc ttagcctttg tatcctcatg
     1261 cacaccctta gcagtaagct cttcctggca cttgttggcg ataaggcaga taatgtgata
     1321 aatctcagga ataacctgtg tcataaggtc agcaggccac ttctcgagag cctcgccgag
     1381 aactgtgtgg tttgtatagt taaatgtctt tcttgcaatt tcaaaagcgt cgtcaaagtt
     1441 gaagccttcg ttcataagaa gtcttacaag ctcgggaaca catgatacag gatgtgtatc
     1501 gttaagctga attgtgtttt ccttagcaaa gaagctgaaa tcattgccgt gcttcttctt
     1561 ataacggcgc ataatgtcct gaagtgatgc agaacagaag aaatactgct gtttaagtct
     1621 gagaaccttg ccctcatact tgttatcgtt aggataaaga acctttgtga tgttttcggc
     1681 atcgttcttt tcctgaacgg cattgttgta ctctgtgttg ttgaaagcag taaaatcaaa
     1741 atcgtttact gcctcagcct gccaaagacg aagtgtgttg atgtttgttg tcttacagcc
     1801 gataacagcc atatcgtaag gaacagcctt aactgtctgg cccttgaatg atactgtaac
     1861 agcctcatct tcaacacggt tgcaccacgg atcttcaaat ctctgccagt tatcagctgt
     1921 ttcaacctga taaccgtttt cgataagctg cttgaaaaga ccgtacttgt aacggattcc
     1981 gtagccgtca agcggtactt cctgtgtagc agcactgtcg aggaaacaag cggcaagtct
     2041 gccgagaccg ccgttaccga gagcggcatc gtcgatatcc tcaaatacat tgatgtcaat
     2101 gcccttcttt ttgaggagct ttgtaacatc ttcaagaatg ccgaggcagt agagattgtt
     2161 gtacatcatt ctgcccatga ggaattctgc agagaaatag taagctctgc gttcgctgtc
     2221 atgtttaatc ttgcttgctt cccagttgcc tgcgatttcg cccattactg ctttgccgag
     2281 tgagtagtga agctcagcgg gtgtgagctc ttcaatcttt ttgcagtaca tagactgagc
     2341 tgtgagttca agcttttcca taatattacc catttgttta gtcctccagt cgtccgcttt
     2401 tttcagacat agtttttaat ttatcagcaa gctcatcagt aagagctgca ccatcgagtc
     2461 tccataccca gtttccgccg agtgttgacg gaatgttcat tcttgcctcc ttgccaaggc
     2521 cgagaatgtc ctgcataggt acgattgctg tatctgcctt gctttcataa gcaacacgga
     2581 tgagtcccca gttgaacggt tcgtcatcag gcatctgaca ataacttctt gcatagttaa
     2641 cgtcctcggg tttagctgtt tcaagccagc ccataacagt atcgttatcg tgagtacctg
     2701 tgtaaacaac acagttttcg gtgtatttat ggggaagata gtcgttctct tcaccactgt
     2761 cgaaagcgaa ttcaagaacc ttcattcccg gatagcctgt ctgctccata agctcggtta
     2821 catcgggagt gagtgttccg aggtcttcgg caacaatatc aatcttgccg agtgcctctt
     2881 ccatcatatt aaagaactta atgcgcggac cttcaaccca ttcaccgttg attgcgtttt
     2941 cggcaggata aggaattgaa tagtagcttg caaaagctct gaaatggtca atacgggtga
     3001 tgtcataaag ctttgatgcg gctttgatac gctcaaacca ccactcatag tcggtagcct
     3061 caaggtaatc ccaatcatag agaggattgc cccaaagctg acctgttgcc gagaagtaat
     3121 ccggcggaca gcctgcaact gcgat
//
LOCUS       DAOMVE010000170         3132 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9141_length_3132_cov_2.626909, whole genome shotgun sequence.
ACCESSION   DAOMVE010000170 DAOMVE010000000
VERSION     DAOMVE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9141_length_3132_cov_2.626909"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1549
                     /locus_tag="PLS64_08250"
                     /pseudo
     CDS             1..1549
                     /locus_tag="PLS64_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523761.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
     gene            1575..2075
                     /locus_tag="PLS64_08255"
     CDS             1575..2075
                     /locus_tag="PLS64_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43849.1"
                     /translation="MKLFITRDVSPSDVCFLIRDELGRDKYTAVMKRRKRSMRGVVNN
                     IVRLNILDENKNLVARLRQLPVAGVNSFTLKTDKTAATLVVLMTNNMVQCRFYGNNWR
                     ILGDVISKNFSIVDVDNAQICNHIKHPLGCELEIADAQNELICLMTALCVNMINTVDK
                     REVQVV"
     gene            2266..2748
                     /locus_tag="PLS64_08260"
     CDS             2266..2748
                     /locus_tag="PLS64_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRL43850.1"
                     /translation="MDKLLKLLSENSGFTTAEIAAMLGEPEDYIKAQIKEYENSGVIK
                     GYQAVVNWDMVDDSYVEALIELQVTPKKETGFDEMAELCMGFEEVESLYLMAGTYDFA
                     LIVRGEAMQDIAMFVAKKLSTIDGVLSTGTHFIMRRYKDRGACLFSDEEKRDGRHLIL
                     "
     gene            2745..>3132
                     /locus_tag="PLS64_08265"
     CDS             2745..>3132
                     /locus_tag="PLS64_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL43851.1"
                     /translation="MIDYNNFINKKISTVKPSGIRKFFDIANEMDDVISLSIGEPDFQ
                     TPWHIREEGINSLKKGKTWYSPNRGFAELREEIAAYYKRRFGIEYDAKKQTLVTVGGS
                     EAIDLVFRTLLDPGDEVIIPEPSFVCY"
BASE COUNT          954 a          611 c          701 g          866 t
ORIGIN      
        1 ccgcagtaat ttataaggga gatatcctcg gcattgtacc taaggtcaat atccctaact
       61 acagcgaatt ttacgaggtt cgtcactaca caagcggtaa gaacctctat gatgaaattt
      121 catatgcaag tgtcgaaacc ataatttcag acaatcttgt gttctgttgt gacaaaatgc
      181 gtgatttcag cttcggtgtt gaagtttgcg aggatttgtg ggttgccgca tctccgtctg
      241 ttgaacacgc aaaacacggc gcaactataa tctgcaaccc gtcaacatcg gatgatgtaa
      301 taggcaaggc acagtatcgc cgtgaccttg taaaaatgca gagcggtaag ctttgttgtg
      361 catatattta cagcgattcg ggctttggcg aaagcacaac ggatatggta ttttcgggac
      421 agaacataat ctccgaaaac gcatctctgc ttgctgaaag caagcgcttt acaacaggca
      481 ttatctatgc cgatattgat gtttgtaagc tttcggcaga acgcagaaag acaaatactt
      541 ttacaaaaag tgatgacaat aacagactcc gttcattccg tcaaacaaat cagatcttga
      601 tgctcgttgt gaggagatta taacaatgca ggcaacaggt cttgcaacaa gacttgctca
      661 cacgggaatt caaaatgcgg ttttaggact ttcaggaggt cttgacagta cgcttgctct
      721 catcgtctgc gttcatgctt ttgatatgct cggtattgac agaaagaata ttcatactgt
      781 aacaatgccg tgtttcggca caacaaagcg tacaaaatca aacgcacagc ttttggctga
      841 ggcttacgga gtgtcttttg aagatatcaa cattacaaaa gctgttcgtc agcattttgc
      901 agatatcaac cacgatgaaa gcgttacaaa tataacctac gaaaactcac aggctagaga
      961 gagaacacag attctcatgg atctttcaaa caaatataac ggtcttgtta tcggcacagg
     1021 cgacctttct gagcttgctc tcggttgggc aacctacaac ggtgaccata tgagtatgta
     1081 tgccgtaaat gtgtcaattc cgaaaaccct tgttcgctat ctcactgcct atgaggcaca
     1141 gcacagcgaa ggagtcctaa aaaccgttct tcttgatgtt ctcgacacac ctgtaagtcc
     1201 cgagcttctt ccgcccgata aaaacggcga aattgcacag aaaaccgaag atgtggttgg
     1261 tccttatgaa ctccacgact ttttcttata ttatttggta agatttggct ttgagccgaa
     1321 taaaatatac taccttgcaa agaaatcatt tgcaggaaaa tacgacaatg ccacaatcaa
     1381 aaagtggctc acaacctttg tcagaagatt ctttactcag cagttcaagc gttcatgtct
     1441 gcctgacgga ccgaaggttg gttctgtaac actttcgcct cgttcagact ggagaatgcc
     1501 gagtgatgcg tgtgtaaatc tttggcttga aaatcttgaa gaagattaag aaataaaatt
     1561 ttgacggtga ttttatgaaa ttgtttatta cacgagatgt atctccgtcc gatgtttgtt
     1621 ttttgattcg tgatgaattg ggcagggata aatatactgc tgttatgaaa cgcagaaaga
     1681 gaagtatgcg tggtgttgtc aacaacattg taaggcttaa tattctcgat gagaataaaa
     1741 accttgttgc aagacttcga cagttgcctg ttgcaggggt aaattcattt accttaaaaa
     1801 cagacaaaac agcggcaacc ctcgtggtgc ttatgaccaa caatatggtt caatgcaggt
     1861 tctacggcaa taattggaga attctcggtg atgtaatttc aaaaaatttc agcattgttg
     1921 atgttgacaa cgcacagata tgcaatcaca tcaagcaccc tttagggtgc gaacttgaaa
     1981 ttgccgatgc gcaaaacgaa ttgatatgtt tgatgaccgc cttatgcgtc aatatgataa
     2041 atacagttga caagcgagag gtacaggttg tataatctat gtgcagacag ttcccaccgc
     2101 taaagccatg tgaactgcca cagagcaagc aaataacagt gcaaacagcg cagataaata
     2161 ttaaaaattg aaattgtcaa agcgacatcc tttgatgtcg ctttttgcaa attcagcttg
     2221 caacgcaaaa ttttgcgttt aatatattct cagggaggaa taaatatgga caaattatta
     2281 aagcttttaa gtgaaaattc cggttttaca acagcggaaa tagcggcaat gctcggcgag
     2341 cctgaggatt acattaaggc gcagattaag gaatacgaaa attcgggggt aatcaagggc
     2401 tatcaggcag tcgtaaactg ggatatggtt gacgacagct atgttgaggc tttaatcgaa
     2461 cttcaggtaa ctcccaaaaa ggaaacaggc tttgacgaaa tggcagaact ctgcatgggc
     2521 tttgaagagg ttgaatctct ctatcttatg gcaggtacat atgactttgc tctcattgta
     2581 agaggggagg caatgcagga tatcgcaatg tttgttgcca aaaagctttc tacaattgac
     2641 ggtgtgcttt caacgggtac acactttatt atgcgccgct acaaggacag aggggcgtgc
     2701 cttttcagcg atgaagagaa gcgtgacggg aggcatttaa ttctgtgata gattacaata
     2761 attttattaa taaaaagatt tcaacggtaa aaccttcggg tatccgtaag ttttttgata
     2821 tagctaacga aatggacgat gtcatttctc tgagtatcgg cgaacccgat ttccagacgc
     2881 cgtggcacat ccgtgaagag ggcattaaca gtctgaaaaa aggtaagaca tggtattctc
     2941 cgaacagagg ctttgccgaa cttcgtgaag aaattgcagc ttactacaaa agaagattcg
     3001 gtattgagta tgatgccaaa aagcagacgc ttgttactgt cggcggcagc gaagcaattg
     3061 accttgtttt caggacattg cttgatcctg gtgatgaggt tatcattccc gaaccttcat
     3121 ttgtttgtta cg
//
LOCUS       DAOMVE010000171         3121 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9180_length_3121_cov_2.894651, whole genome shotgun sequence.
ACCESSION   DAOMVE010000171 DAOMVE010000000
VERSION     DAOMVE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9180_length_3121_cov_2.894651"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLS64_08270"
     CDS             <1..691
                     /locus_tag="PLS64_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRL43852.1"
                     /translation="LSEKVNFSQLLREVDSIDGDYWLRFMTSHPKDCSKELIDTIASG
                     THISKHLHLPFQCGSDRVLKAMNRHYDRRKYLEIINYAKEKIDGLSLTSDIIVGFPGE
                     TYEDFKETLSLIREVEFTSLFTFIFSPRVGTPAAKMDDPISAEEKSKWFRELLDVQEE
                     IAAKRCSSMVGKTEKVLIEGENDKNGELCGRTSGNIIVELEGSKDCIGTFRNVKITKA
                     RNWILKGELVK"
     gene            707..1114
                     /locus_tag="PLS64_08275"
     CDS             707..1114
                     /locus_tag="PLS64_08275"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRL43853.1"
                     /translation="MDIIEKARELGRLIQEEDSYKKLQDAQKNADADMELQRLIGEFN
                     LKRMSINNEASKKERDQEKLSKLNTEMREAYSQIMSNENMIAYNDAKAAFDVVANRVL
                     AIVQQSAEGADPETADYSQSSCSGSCATCGGCG"
     gene            1169..>3121
                     /gene="mutS"
                     /locus_tag="PLS64_08280"
     CDS             1169..>3121
                     /gene="mutS"
                     /locus_tag="PLS64_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRL43854.1"
                     /translation="MMQQYFKIKEENKDSILFFRLGDFYEMFYDDAKLASKELELTLT
                     GRDCGQAERAPMCGVPFHSCEGYIARLVAKGYKVAICEQTEDPAKAKGLVKRDIIRVI
                     TPGTVMESSMLDESKNNYICCMYSKNKTIGLCFCDISTGELYATEIRGHDSYNVLTNQ
                     LTSYNPREILIGGDIVKLKELPKFIKAKLSAGVEMLEDEKFDESVCTDVVKSQFADEY
                     STVSDKSVVVCVLGALLSYLRDTQKTGLERINHIEFYKENQYMSLDYNTQRNLELTQT
                     MLTKDKKGSLLWVLDKTKTAMGKRLMRSWLEHPLLNITSINNRQSAIEELVNDNMLRM
                     DVTDTLSGIFDIERLMTRIVYGSANARDLRSLCGAIQNLPQISDLLVDCKSVYLKYIY
                     KSIDKLEDIHSLIDSAIVEEPPFTVREGGMIKRGYNEELDSVTGDMNDSKGILARIEA
                     EQRDITGIPKLKVGYNRVFGYYIEVSNSYKSMVPETYIRKQTLTNCERYITQDLKDVE
                     GRILGAKDRSVALEYNLFDDVRKTVSDNLERIQKTAKAIANLDVITSLANVAADNRYI
                     RPDVNLSTAIRIKDSRHPVVEALLKDAPFVPNDVSLDSANDRVAIITGPNMAGKSTYM
                     RQIAIIVLMAQIGSFVPASSAEIGVVD"
BASE COUNT         1001 a          562 c          694 g          864 t
ORIGIN      
        1 tctttccgag aaagtaaact tttcacagct tttgcgtgag gttgattcaa ttgacggcga
       61 ttattggctt cgctttatga catctcaccc gaaggattgt tcaaaggaac ttattgacac
      121 aattgcatcg ggtactcata taagcaagca tcttcacctt ccgttccaat gcggttcgga
      181 cagagtgctt aaagcaatga accgtcacta tgaccgcaga aagtatcttg aaataatcaa
      241 ttatgctaaa gagaaaattg acggtctttc acttacaagt gatatcattg taggttttcc
      301 gggtgaaaca tatgaagatt tcaaagaaac tttatcgctc atccgtgagg ttgagtttac
      361 atcactcttt accttcattt tctcaccgag agtaggtact cctgctgcta aaatggacga
      421 tccgatttcc gctgaagaaa aatcaaaatg gttccgtgaa cttcttgatg ttcaggaaga
      481 aattgcggca aagcgttgtt cttcaatggt tggcaaaacc gaaaaggtac ttattgaagg
      541 tgaaaacgat aagaacggtg aattgtgcgg tcgtacaagc ggtaacataa tagttgagct
      601 tgaaggatca aaggactgta tcggaacatt cagaaatgta aaaataacaa aggctcgcaa
      661 ctggatatta aagggtgagc ttgtaaaata atcggaggtt aaaactatgg atatcattga
      721 gaaagcaagg gaactcggaa gactcattca ggaagaagac tcatataaaa aattgcagga
      781 cgctcagaag aatgcagatg cagatatgga gcttcagcgt cttatcggtg aattcaatct
      841 taaaagaatg tcaataaaca atgaagcatc aaaaaaagag agagatcagg aaaagctttc
      901 taagcttaat accgagatga gagaggcata ttctcagatt atgtcaaacg agaatatgat
      961 tgcctacaat gatgcaaagg ctgcttttga tgttgttgca aacagagttc tcgcaattgt
     1021 tcagcagtct gctgagggtg cagatcccga aactgctgat tacagccagt catcatgctc
     1081 aggcagttgt gctacatgcg gcggttgcgg ctgataaacg gttctaaatt ttgttaagga
     1141 agtgaaacaa atggcggagt tatctccaat gatgcaacag tatttcaaaa ttaaagaaga
     1201 aaacaaagac agcattttgt tcttccgact tggagatttc tacgaaatgt tttacgatga
     1261 tgcaaagctt gcatcaaagg agcttgaact tactcttaca ggaagggatt gcggacaggc
     1321 cgagcgtgct cccatgtgcg gagttccgtt tcacagctgt gagggttaca ttgcaagact
     1381 tgttgcaaag ggctataagg ttgcaatttg cgaacagacg gaagatcctg caaaggcaaa
     1441 aggtcttgta aagcgtgata ttattcgagt aattactccc ggtaccgtaa tggaatcgag
     1501 tatgcttgat gagagcaaaa acaactacat ttgctgtatg tattctaaaa ataaaactat
     1561 aggactttgt ttttgcgata tttccacagg agaattatac gctacggaaa tcaggggtca
     1621 tgacagctat aatgtcctta ccaatcagct tacaagctat aatccccgtg aaattcttat
     1681 tggcggtgac attgtaaagc tgaaagagct gccgaagttc attaaagcga aactttcggc
     1741 gggagttgaa atgctcgaag atgaaaaatt tgacgagtcg gtatgtactg atgttgtaaa
     1801 atcacagttt gcggatgaat acagtaccgt atctgacaaa tctgttgttg tatgcgttct
     1861 cggtgcattg ctttcatatc ttcgtgatac tcagaaaacg ggacttgaaa gaataaatca
     1921 cattgagttc tacaaagaaa atcaatatat gagccttgat tacaacacac agcgtaatct
     1981 tgagctcaca caaacaatgc tgacaaagga taaaaagggt tcacttttgt gggtgcttga
     2041 caagacaaaa accgcaatgg gtaaacgcct tatgcgttca tggcttgaac atcctttgct
     2101 taatataact tcaattaaca accgtcagag tgcgattgaa gagcttgtaa acgataatat
     2161 gctccgtatg gatgttacag acacactctc gggtatattt gacattgaaa gacttatgac
     2221 aagaatcgtt tacggttctg caaatgcaag agatttgcgt tcactctgcg gagcaattca
     2281 gaatcttccg cagatttcgg atttgctcgt tgattgcaaa tcagtctatc ttaaatatat
     2341 ttataaaagc attgataagc ttgaggacat tcattctctt attgattctg caatcgttga
     2401 agaacccccg tttacagtcc gtgagggcgg tatgattaag agaggttata acgaagaact
     2461 tgattccgtg acaggcgata tgaatgactc aaagggaatt cttgcccgta ttgaggctga
     2521 acagcgtgat ataacgggaa ttcctaagct taaagtcggt tataacaggg tattcggata
     2581 ttatattgaa gtatcaaatt catataaatc catggtaccc gaaacataca taagaaagca
     2641 gacactcacc aattgtgaaa gatatattac tcaggatttg aaagatgtag agggcagaat
     2701 cctcggtgca aaagacagat cggttgcact tgaatacaac ctctttgatg atgtcagaaa
     2761 gacggtttcg gataatcttg aaagaattca gaaaaccgca aaagccattg ctaatcttga
     2821 tgttattaca tcacttgcaa atgttgcggc tgacaacaga tacataagac cagatgtaaa
     2881 cctttcaacc gctataagaa ttaaagattc gagacatccg gttgttgagg ctctattaaa
     2941 ggatgcaccg tttgttccga atgatgtcag ccttgattct gccaatgaca gagtagcaat
     3001 tattacaggt cccaatatgg cgggtaagtc aacctatatg cgacagattg ccattattgt
     3061 tcttatggcg cagataggca gctttgttcc ggcgtcttct gccgaaatcg gagttgttga
     3121 c
//
LOCUS       DAOMVE010000172         3096 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9281_length_3096_cov_3.309438, whole genome shotgun sequence.
ACCESSION   DAOMVE010000172 DAOMVE010000000
VERSION     DAOMVE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9281_length_3096_cov_3.309438"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1817)
                     /gene="ade"
                     /locus_tag="PLS64_08285"
     CDS             complement(96..1817)
                     /gene="ade"
                     /locus_tag="PLS64_08285"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRL43855.1"
                     /translation="MLEIYGQKTDRQLAAKQRIIAVAAGREKADLVLKNAKYLNVFSN
                     EFLCGDIAVANGLIAGVGKYDGKTEIDVSGKLVLPGFIDAHIHLESSMVTPAEFAKAV
                     VAHGTTTVITDPHEITNVMGIDGVEYMIQASQNLPIDVHFMMPSCVPATEIDESGAEL
                     DCKDIDLYLDNKKVLGLAEMMNYVGVINGDKNVLSKIVTSQAHHKKIDGHAPELSGND
                     LNAYIAAGVYSDHECSTFENALEKLRKGQFIMIREGTAAHNLKALMPLLTQQYYSRCM
                     FATDDKHPSDLLYGGHIDYIVKQALKNGADPIVALKTATHHAARYFLLNNKGAIASGY
                     LADIVVVDNLEDFNVETVFKCGKLVFDGEVKDFSAPTVDEKLAEKCFDTFHLDSVTPS
                     SFKVDGKLGLIGLVGGELLTRNLGTADKIDVENDILKIACIERHKGTNHIGVGYVKGY
                     SLKSGAVATSVAHDSHNIITVGCNDDDIAVAVNAIKDSKGGIAVVENGKIKALLELPI
                     AGLMSDEPLTTVNEKLENAKSSAYELGADKSIDPFMTLSFLSLPVIPSLRITTKGVFD
                     AENWKML"
     gene            2054..>3096
                     /locus_tag="PLS64_08290"
     CDS             2054..>3096
                     /locus_tag="PLS64_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HRL43856.1"
                     /translation="MSHTYSEFLPSYSVGVDCYKAIPEIVKPYGKKAVAVGGKTAMEK
                     SKKALLEGIKGSDIEIIDFVWYGGDSSYENVEKIKNNPSVQKADMVFGIGGGRAVDTA
                     KTYCALYNKPLFTFPTIASNCASCTSLSVMYNPDGTFKEYSYHKAPAIHTFINTKIIA
                     ESPEKLMWAGIGDALSKEYEVIFACREKNMIHTPLLGKAIASASTEPLINFGKKALED
                     CKNNVPTEAIEQVALDIIITAGLVSNFTVHENNPNPEEDYYYNSSLAHCVYYGASLFP
                     QCERQHLHGEIVSFGVLCLLTYDGQFEERNRIFEFNHSIGLPCTLGEIALTPDDVPAI
                     AHKAASVVEWKYVP"
BASE COUNT          873 a          675 c          647 g          901 t
ORIGIN      
        1 acaaaataaa acaggaaaac aaagtgacaa tacgcatttt gttttcctgt ttaaattatt
       61 ttatgtttta ttgtattatt aaaagcttcc gcacatcaga gcattttcca attttctgca
      121 tcaaacacac ctttggtggt tattctcaag cttggaataa cgggcagact tagaaacgag
      181 agagtcataa acggatcaat gcttttatcc gcaccgagtt catatgcaga tgactttgca
      241 ttttcaagct tttcgtttac ggttgtcagc ggttcatcgg acatcaagcc tgcaatcggg
      301 agttcgagca aagccttgat tttgccgttt tcaacaactg cgataccgcc cttgctgtcc
      361 ttgattgcgt tcacggcaac ggcaatatca tcatcattac agccgactgt gataatgttg
      421 tgcgaatcgt gggcaaccga ggttgcgact gcgcccgatt tcagcgaata gcctttaaca
      481 tagccgacac cgatatgatt tgtgcctttg tgacgctcaa tgcaggcaat tttcagaata
      541 tcgttttcca catcaatttt atctgctgtg ccgagattgc gtgtgagcaa ttcaccgccg
      601 acaagaccga taagtccgag ttttccgtca accttgaaac tgctcggagt tacgctgtca
      661 aggtggaaag tgtcaaagca tttttcggca agcttttcat caactgtggg agcagaaaag
      721 tccttaactt cgccgtcaaa aacaagctta ccacacttga aaacagtttc aacattgaaa
      781 tcttcaagat tatcgacaac aacaatatcg gcaagataac ccgaagcaat cgcacctttg
      841 ttattgagca aaaagtatct tgcggcatgg tgggttgctg ttttgagggc aacaatcgga
      901 tctgcaccgt ttttcagcgc ctgttttaca atgtagtcaa tatgaccgcc gtagagcaaa
      961 tcgcttggat gcttatcatc tgttgcaaac atacaccttg agtaatactg ctgagtcaaa
     1021 agcggcataa gtgccttgag attatgagcg gcagtaccct ctcttatcat aataaactga
     1081 ccttttctca gcttttcaag ggcattttca aaggtggaac actcgtggtc ggaatagaca
     1141 cctgcggcaa tgtatgcgtt gaggtcattg cccgaaagct caggagcgtg accgtcaatt
     1201 ttcttgtgat gagcctgcga ggtgacaatt tttgaaagca catttttatc gccgttgata
     1261 acaccgacat agttcatcat ctctgcaagt ccgagtacct ttttattatc caagtacaag
     1321 tcaatatcct tgcagtcaag ctccgcaccg ctctcgtcaa tttctgtggc aggcacacat
     1381 gacggcatca taaaatgaac atcaatcggc aaattttgcg atgcctgaat catatattca
     1441 actccgtcaa tgcccattac atttgtgatt tcgtgagggt cggttatgac tgtggttgta
     1501 ccgtgggcaa caactgcctt tgcaaattca gcaggagtca ccattgaact ttcaaggtga
     1561 atatgtgcgt cgataaagcc ggggagaacg agttttccgc tgacatcaat ctctgttttg
     1621 ccgtcatatt ttccgacacc ggcaatcagt ccgtttgcaa cggctatgtc accgcacaaa
     1681 aattcatttg aaaacacatt aagatacttt gcgtttttta aaacaaggtc tgccttttct
     1741 ctgcctgccg caactgcaat tattctctgc tttgctgcaa gctgtctgtc tgttttctgt
     1801 ccgtatattt ccagcataaa agcaccgccc ttcttatata tatttacaag attatagcac
     1861 tttgcaaaag ccatttccat aagtattttt cacgaatatt cttcgcaatt ttttgctaaa
     1921 ttgcataaga tgtgtgcata ttttcatctg tcaggcaata ataattttaa caatttattg
     1981 tgctaatgta ttgaaatgta caaactgcgg tgatataatt tatttataag tattgttttc
     2041 ggagggataa attatgtcac acacatacag cgaattttta ccgagttaca gcgtgggagt
     2101 tgattgctac aaggcgattc ccgaaattgt gaagccgtac ggcaaaaagg cggttgcagt
     2161 cggcggtaaa acagctatgg aaaaatcaaa aaaggctttg cttgaaggca tcaaaggctc
     2221 tgacattgaa atcattgatt ttgtatggta cggcggtgac tccagctatg aaaatgttga
     2281 aaaaataaag aacaatccgt ctgtgcaaaa ggctgatatg gtgttcggta tcggaggcgg
     2341 acgagctgtt gacacggcaa aaacctattg tgctttatat aacaaaccgt tgttcacatt
     2401 cccgacaatt gcatcaaact gtgcgtcatg cacctcgctt tcggtaatgt acaatcccga
     2461 cggtactttc aaggaatatt cgtaccacaa agcccctgca attcacacat ttatcaacac
     2521 aaaaattatt gccgaatcac ccgaaaaact tatgtgggca ggaatcggtg acgcactttc
     2581 aaaggaatat gaagtaattt ttgcgtgtcg tgaaaagaat atgattcaca caccgttgct
     2641 tggcaaggca attgcctccg caagtaccga accgctcata aactttggca aaaaagctct
     2701 tgaggactgc aaaaacaatg tgccgacaga ggcaattgag caggttgcgc ttgacattat
     2761 tatcacggcg ggacttgttt caaattttac cgtacacgaa aacaatccga atcccgagga
     2821 ggattattac tacaattcat cgctcgcaca ttgtgtttat tacggtgcgt cactctttcc
     2881 gcaatgtgaa cggcagcatc tgcacggcga aattgtttca ttcggtgtgc tgtgtctgct
     2941 gacatatgac ggacaatttg aggaacgaaa cagaattttt gaatttaacc acagtatcgg
     3001 acttccttgc actctcggcg aaattgcact tactccggat gatgtgcctg caattgcgca
     3061 caaagccgca agtgttgttg agtggaagta tgtccc
//
LOCUS       DAOMVE010000173         3012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9621_length_3012_cov_2.919851, whole genome shotgun sequence.
ACCESSION   DAOMVE010000173 DAOMVE010000000
VERSION     DAOMVE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9621_length_3012_cov_2.919851"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PLS64_08295"
     CDS             <1..223
                     /locus_tag="PLS64_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRL43857.1"
                     /translation="STDFTERELEVLRELTSGDTNTEIAKRLYIAPGTVKNYIQYMLE
                     KTGFKSRTELAVKAREAGLVILDDRINDK"
     gene            409..810
                     /locus_tag="PLS64_08300"
     CDS             409..810
                     /locus_tag="PLS64_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43858.1"
                     /translation="MEAKGSTFLKVTGILMIVFGAIALVVSIIAILGIAALAAFNDGT
                     YDMTMLYVGGVFALISAVAEFVAGIIGVINAKLPHKANTCIVWGVVVAVMCIAGEIIS
                     MIGGSQFNVFSLICGLAIPVLYIIGAVKNKA"
     gene            1004..1390
                     /locus_tag="PLS64_08305"
     CDS             1004..1390
                     /locus_tag="PLS64_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43859.1"
                     /translation="MKKILLSVQNNLLSEAIQNALVKKGRFCIENVSPNQDGKILDLC
                     IAERVDVLLMDVTRLADSTIEKRLELCGKVKEYLPSCKTALLCDEQAYPDLADRVKQA
                     KQFGKISSFFYSSVTADYLAAALDAL"
     gene            1407..1991
                     /locus_tag="PLS64_08310"
     CDS             1407..1991
                     /locus_tag="PLS64_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43860.1"
                     /translation="MGIFDSLKNMTNQAVKNNVSKAVSGAVNNAMHKSKTFVFADYPK
                     NADELKKMPELDFSSPLSTAAFAMLVLLEYDESPENTIEMLNVLKGPQPMNGMDVQFL
                     RDRIKGRGYIPRSYFEGSSVKNDYTPNVPYKITVSEYAYTYQSEGYAKVQVQSSGADS
                     PRPIELRRKGNQWFLWRNLALSDIRTPASVDPWA"
     gene            2005..>3012
                     /locus_tag="PLS64_08315"
     CDS             2005..>3012
                     /locus_tag="PLS64_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43861.1"
                     /translation="MKIKKKVKRISSVVLCFLMLLTTLPMTAITANAAAQAYIKYIDT
                     EVYIGDVLNIIVGVNGGSTDETFTYQWQAKYPSLNWVNLSDKTDRPNSKFSGVFTDHL
                     KFQTYAELVGPDSGWKDVVFRCKVTGSKSGTFYSGKCNFPGLLEKTEIPIIGFLGLEK
                     PEQRKIPSTTATPINSTYYSFDYIEWYEYKNNKVSRKLNDGEAFDSGMYCCQLYFNMN
                     RGYAVAKNAVCGLYNDDGQATILQDETTGQYYIWAYYNVPYESPSFTHQFPEAEIMIM
                     KGKEEIIWVAAKNAASYQWQMKVKRRTPTGIIRMWKNIDDDSSTLNQFSFKGTKSNTL
                     RI"
BASE COUNT          941 a          554 c          661 g          856 t
ORIGIN      
        1 gagtacggat tttaccgaaa gggagcttga ggttttgcgt gaactgacaa gcggtgatac
       61 aaacacggaa attgccaaaa gactttacat tgctccgggt actgtcaaaa actatataca
      121 gtatatgctt gaaaaaacag gctttaagtc aagaacagag cttgccgtaa aggcaagaga
      181 ggcgggactt gtaatccttg acgatagaat taacgacaaa taaatacttt tttctgaata
      241 tgtgccgttt ggcacatatt tttttgctgt taaaacagta taatacaatt acaggatata
      301 aactgctcag acggtgaata tcttttgtgc aaaacgctat ctttgtccga ttaataatca
      361 aagacaaagg tgcataaaat ttattattta atgacaggag gaaaaactat ggaggcaaaa
      421 ggttcaactt ttcttaaagt aacgggaatc cttatgattg tattcggtgc tattgcattg
      481 gttgtgtcaa tcatcgccat tctcggtatc gccgcacttg cagcttttaa cgacggtaca
      541 tacgatatga ctatgcttta tgtcggcggc gttttcgcac ttatcagtgc ggttgctgag
      601 tttgttgcag gtattatcgg cgttatcaac gcaaaacttc cgcacaaggc aaatacatgt
      661 attgtatggg gcgttgttgt tgcggttatg tgtattgcag gcgaaattat cagcatgata
      721 ggcggatcac agttcaatgt attctctctt atctgcggtc ttgctatccc tgtactttac
      781 attataggcg ctgttaagaa caaagcataa ttattttatt atttaccccc ctttttaatt
      841 acccaccgta aatattttta gcacaaggtc acagctgtcc gaacaatcgg gcagctgtgc
      901 tttttttgtt tatgttgtga aatatgccgt ttggcacatt catattctat cgcaaaattg
      961 tacaatataa gtgaaagata taggtgaaaa ggggtgatgt ccgttgaaga aaattttgct
     1021 cagtgttcag aataatttgc tcagtgaggc tattcaaaat gcccttgtaa agaagggaag
     1081 attttgtatt gagaatgttt caccgaatca agacgggaaa attcttgatt tgtgcattgc
     1141 agaaagagtt gatgttttac ttatggatgt cacaagactt gccgattcaa ctattgagaa
     1201 acggcttgag ctgtgtggaa aagtaaagga atatctgcca agctgtaaaa cggctctgct
     1261 ctgtgacgaa caggcatatc ccgaccttgc cgacagggtt aaacaggcaa aacaattcgg
     1321 aaaaataagc tcgttttttt attcatccgt aacggcagat tatcttgccg ccgcacttga
     1381 cgcactttaa aaaggaggta ctgtttatgg gtatatttga cagcttaaaa aatatgacaa
     1441 atcaggctgt aaaaaacaat gtttcaaagg ccgtttcggg agcggtaaac aatgcgatgc
     1501 acaaaagcaa aacatttgta tttgccgatt atccgaaaaa tgcagatgaa ctcaaaaaaa
     1561 tgccggagct tgacttttcg tcacctcttt caacagcggc atttgcaatg cttgtgttgc
     1621 ttgaatatga tgaaagtccg gaaaacacaa tagaaatgct taatgtatta aaaggacctc
     1681 agccgatgaa cgggatggat gttcagtttt tgcgtgacag aatcaagggc aggggatata
     1741 ttcctcgctc atattttgag ggttcatccg tgaagaacga ttatacaccg aatgttccgt
     1801 acaaaataac cgtttccgag tatgcatata cctatcagag cgagggttat gcaaaggttc
     1861 aggttcagtc gtcgggagca gactcacctc gtccgattga gcttcgcaga aagggcaatc
     1921 aatggttctt atggcgtaat cttgcactct ctgatatacg aactccggct tcggttgatc
     1981 cttgggcatg aaggagggat ttttatgaaa atcaagaaaa aagtaaagcg tatttcgagt
     2041 gttgttttat gctttctcat gctgttgaca acacttccta tgacagccat aaccgcaaat
     2101 gcggcggctc aggcttatat caaatatatc gacaccgagg tttacatagg agatgtcctt
     2161 aacattatcg tgggagtaaa cggcggcagt acagacgaaa cctttaccta tcaatggcag
     2221 gcaaaatatc cgagcctaaa ctgggttaac ctctccgata aaaccgacag acccaactcg
     2281 aaattttcgg gtgtttttac agaccatctt aaatttcaga cctacgcaga acttgtagga
     2341 cctgactcag ggtggaaaga tgttgtcttt agatgtaaag taacgggttc aaagagcggt
     2401 actttttatt cgggaaaatg taactttccg ggacttcttg agaagacaga aattcctata
     2461 attggatttt taggcttgga gaaacctgaa cagcggaaaa tcccctcaac tacggcaact
     2521 ccgataaaca gcacttacta cagttttgat tatattgagt ggtatgagta caaaaacaac
     2581 aaagttagca gaaagctcaa cgacggagaa gcttttgaca gcggtatgta ttgttgtcag
     2641 ctatacttta atatgaacag aggatatgcg gttgccaaga acgcagtatg tggcttgtac
     2701 aacgacgacg gtcaggcgac aattcttcag gatgaaacca caggtcaata ttatatttgg
     2761 gcatattaca atgttccata tgaatcgcca tcatttacac atcagttccc tgaggcagaa
     2821 attatgatta tgaaaggcaa agaagaaatt atatgggttg ctgccaaaaa tgccgcaagc
     2881 tatcagtggc agatgaaagt aaagagaaga acacccaccg gtataataag aatgtggaaa
     2941 aacatagatg acgactcatc tacattaaac cagttttcct tcaagggtac aaaatcaaat
     3001 actttgagaa tc
//
LOCUS       DAOMVE010000174         3003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9657_length_3003_cov_3.843962, whole genome shotgun sequence.
ACCESSION   DAOMVE010000174 DAOMVE010000000
VERSION     DAOMVE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9657_length_3003_cov_3.843962"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..1553)
                     /locus_tag="PLS64_08320"
     CDS             complement(102..1553)
                     /locus_tag="PLS64_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRL43862.1"
                     /translation="MFIGRETELKFLNDKYNSDGGQLVVLYGRRRVGKTETLREFCKE
                     KPHVFFSCTQTTDRVQLQKFSKQILKENIPAKNYITEFADWEKAFGAVLDLPYGDKKK
                     LFIIDEFPYMCKGNKSIPSILQNLWDTQLRDSNVMIVLCGSAMSFIEKEILAEKNPLY
                     GRATGIFKMKEMGFYDAAKFFTAYSERDKVIAYSVLGGIPHYLRQWNPKLTIEENIKQ
                     NILTKGCVLYSEVDFLLHQELRETPVYNSIIEAVALGNTTLNNISQKSLIEDTSKTSV
                     YLKNLIELGIVEREFSVDAKIKEQANGNRGTYKLTDNFFRFWYAFGFANFSQLEDGDV
                     DGVYEYIVKPELHKFASVAFEDVCREFVRKMQKNNELPFRYSKMGRWTGKTTIRDKNT
                     ANGLRIGETEIDILCIDQKAENYLVGECKFKKEAFTYAEYLNTLAKLTPLKEKTQFYY
                     ALFSENGFDEKIKADTKSSGTLLFSLEQIVNYS"
     gene            complement(1810..>3003)
                     /locus_tag="PLS64_08325"
     CDS             complement(1810..>3003)
                     /locus_tag="PLS64_08325"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522855.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRL43863.1"
                     /translation="ANKKFTLAGKEFHEGDYISIDGSTGNIYDGVIPTVDATIAGEFG
                     RIMAWADKYRTLKVRTNADTPADAKKARELGAEGIGLCRTEHMFFEEDRIAAFREMIC
                     SDTVEEREAALDKILPYQQGDFEALYEALEGNPVTIRFLDPPLHEFVPTEEADIEKLA
                     AAQGKSVEDIKTIIASLHEFNPMMGHRGCRLAVTYPEIAKMQTKAVIRAAINVQKAHA
                     DWTVEPEIMIPLVCDVKELKFVKKVVVETADAEIAAAGIDLKYEVGTMIEIPRAALTA
                     DEIATEADFFCFGTNDLTQMTYGFSRDDAGKFLNAYYDAKIFENDPFAKLDQTGVGKL
                     METAIKLGKPVNNKLHVGICGEHGGDPSSVEFCHKIGLDYVSCSPFRVPIARLAAAQA
                     AIANK"
BASE COUNT          833 a          629 c          581 g          960 t
ORIGIN      
        1 tcttataaaa agcactctgc aaacgcttag gcttaattat atttttgcta ttattatacc
       61 tcatattcga gagcggtttt taggttaatt caaaatatat gtcaactgta attgacaatt
      121 tgttcaaggg aaaagagaag tgtgccactt gattttgtat cggcttttat cttttcatca
      181 aaaccgtttt cagagaacaa tgcataataa aactgtgtct tttccttcaa tggtgttaat
      241 tttgcaagtg tgttcagata ttctgcatag gtaaatgctt cttttttgaa tttgcattcc
      301 ccaactaaat aattttctgc tttttggtcg atacataaaa tatctatttc tgtttctccg
      361 attctaagtc cgtttgctgt gtttttatcc cttattgttg tctttcctgt ccaccgtccc
      421 attttggaat atcggaacgg cagttcgtta tttttctgca ttttccgaac aaactcacgg
      481 catacatctt caaatgcgac tgatgcaaat ttatggagtt cgggctttac aatatattcg
      541 taaacgccgt caacatcgcc gtcctcaagc tgtgaaaaat ttgcaaaacc gaatgcatac
      601 cagaatcgga agaagttatc tgttaattta tatgttcctc tgttaccgtt tgcctgttct
      661 tttatctttg catcaacaga aaattctcgt tccacaatgc cgagttcaat cagatttttt
      721 aaataaacgc ttgttttgga agtatcctca atgagtgact tttgactgat attgtttaaa
      781 gttgtatttc caagtgctac cgcttcaatg atagagttat atacaggcgt ttcccgaagt
      841 tcctgatgaa gaagaaaatc aacctcgctg tatagaacac aacctttggt caaaatgttt
      901 tgtttaatat tttcttcaat agttagtttc ggattccatt gccttaaata gtgggggata
      961 ccgccaagaa cagaataagc aataactttg tcccgttcgg aatatgcggt aaagaattta
     1021 gctgcatcgt aaaatcccat ttctttcatt ttgaaaattc cggtagcacg accgtagagc
     1081 ggattttttt cggcaagaat ttccttttca ataaaactca ttgcactgcc gcacaaaaca
     1141 atcataacat ttgaatcccg aagttgtgta tcccacaaat tttgcagaat agaaggaatg
     1201 ctcttgttcc ctttgcacat atacgggaat tcatctatga taaagagttt ctttttatcc
     1261 ccatacggca aatcgagaac agcgccgaaa gctttttccc aatcggcaaa ttcggtaatg
     1321 taattttttg caggaatgtt ttcttttaat atttgctttg aaaatttttg aagttgcacc
     1381 ctgtcggttg tctgagtaca ggaaaagaat acatgtggtt tttctttgca aaattctctt
     1441 aaggtttccg ttttgccgac acgccgtctg ccatacaaaa ctacaagctg tccgccatct
     1501 gaattgtatt tatcgttcag aaattttagc tcggtttctc tgcctataaa cataaaaaca
     1561 cctcttatgc tatctaatct tgatttacta aatcgtgatt tggtattatt atacccaaga
     1621 acaaatgtaa aatcaataga ttttgcaaaa acaataaaaa ttttcaaaat acgccgttat
     1681 ataaattgtg ggaaaccttt aaagattttt ataagacata aaaagacctt gcagagtaaa
     1741 atctgcaagg tcaaatttat atcaggaata aacttgtatt aagttacctg tcaatacatc
     1801 ctgtgtggat tacttgtttg cgatagcagc ctgagcagca gcaagacgag caatcggaac
     1861 acggaacggt gaacatgata catagtcaag accgatcttg tggcagaact caacagaaga
     1921 aggatcgccg ccgtgctcac cgcagatacc aacatggagc ttgttgttta caggcttacc
     1981 gagtttgata gctgtttcca tgagcttgcc aacgcctgtc tggtcaagct tagcgaacgg
     2041 atcgttctcg aagatcttag catcatagta agcgttgagg aacttacctg cgtcatctct
     2101 tgagaagccg tatgtcatct gtgtaaggtc gttagtaccg aagcagaaga agtcagcttc
     2161 cgtagcaatc tcatcagctg taagagcagc tcttgggatt tcgatcattg taccaacttc
     2221 gtacttgagg tcgatgcctg cagcagcgat ttcagcgtca gctgtttcaa caacaacctt
     2281 cttaacgaac ttgagttcct taacatcgca tacgagcgga atcatgattt caggctcaac
     2341 tgtccagtct gcgtgtgcct tctgaacatt gatagcagca cggataactg cctttgtctg
     2401 catctttgca atttcaggat atgttactgc aagacggcaa ccacggtgac ccatcattgg
     2461 gttgaactcg tggagtgaag cgatgattgt cttaatgtct tcaacgctct taccctgagc
     2521 agctgcgagc ttttcgatgt cagcttcctc tgtaggaacg aactcgtgaa gcggcggatc
     2581 aaggaatctg attgttacag gattgccttc gagtgcctca taaagagcct caaagtcgcc
     2641 ctgctgataa ggaagaatct tgtcaagagc agcttctctt tcttcaactg tatctgagca
     2701 gatcatctct ctgaatgcag cgattctgtc ctcttcaaag aacatatgct ctgtacggca
     2761 aagaccgata ccttcagcac cgagctcacg agccttctta gcgtcagcag gtgtatcagc
     2821 gtttgttcta accttaagag ttctgtactt gtcagcccaa gccatgattc tgccgaactc
     2881 acctgcgatt gtagcgtcaa ctgttgggat aacgccgtcg tagatgttac ctgtagaacc
     2941 gtcgattgaa atgtagtcac cctcgtggaa ttccttgcct gcgagtgtga acttcttgtt
     3001 agc
//
LOCUS       DAOMVE010000175         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9664_length_3002_cov_2.952155, whole genome shotgun sequence.
ACCESSION   DAOMVE010000175 DAOMVE010000000
VERSION     DAOMVE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9664_length_3002_cov_2.952155"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="PLS64_08330"
     CDS             <1..649
                     /locus_tag="PLS64_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43864.1"
                     /translation="FVMCFSVVTTAFAATPDEISESDVASEIRQVMIDTYYGGKEPEY
                     LNIEIVGSTSDGGVYFMHNEGFSLDYSIEETIGDYTYHYNHNDDVMLYKNGNVYSIKE
                     AYESGVIDDSILEELSKMNFGLESNKPNTTVPTEPTTSVATVDEPVTVPDTTSATSAT
                     KPVSTSDTATNDTPNNSQNNSGAVQTGQNSVAVILLVAMLAGCAVIFAKRRNYFK"
     gene            846..2987
                     /locus_tag="PLS64_08335"
     CDS             846..2987
                     /locus_tag="PLS64_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRL43865.1"
                     /translation="MKKFLPIAMAAVMSMSAVAVSAVSANAVEPLNTPVQYAQVARAS
                     LATPKISKAESVYGGVKLTWNKVSGATKYRVYYKGRKGWTRMVDTTSTSYIDKDVSSG
                     RNYTYTVRCISADGKSFTSGYDSKGTTVKYVAAPEISKLENVNGGVKITWNKVSGASK
                     YRIYQKNSSDWFRVSDTTSNSIVDKSVDVGTYTYTVRCISDDGKSFESGFNPKGLSIR
                     YNQAPKILETNVVNGGIKVIWDSEENTNYRLYCKTEGKGWTKVCDSKTGVVTHKNLQP
                     NKTYTYTVRAISSDAKKYLSGYDPVGMSAKYVATPKISKTEVTYDGVKLTWNKVAGAE
                     KYRVYYKDGEGWNKLADTTGNSYLDMGLVSRELSIKDNSVNGQYIYTVRCISSNGKVF
                     QSGYDTKGSKANLGNECPEIVRVDSMHGGITVSWTDVCEDNSKYRIYVKEKGSWKKLA
                     DTTNNYYTHKNVKAGQTYTYTVRCVSKDGKKFTSIYNPSGVTFKYKTTDKTRDAYIDY
                     LLKHESEWLPELKVGNVALAGVQFADLDFDGVPELIVQEAGDKEYYLNAKVYTFKDGK
                     FSLVGFDDYRTYIPNQLTYFYDKSAKKYIINAVELSTSNVDRYLERENYTLNYDGTVL
                     VVRGYSSAHLALESRGLEYHYYNNTGRTTKQAYNATNVSVLGNCVNAHMYSQFVHCSV
                     DGYPKLWNNYSETQKKQHLLDSYNAFSYDKY"
BASE COUNT         1018 a          487 c          639 g          858 t
ORIGIN      
        1 atttgtaatg tgcttttcgg ttgtaacaac agcttttgcg gctacacctg atgaaatatc
       61 agaatcggat gttgcatcag aaatcagaca ggtgatgata gatacatatt acggtggtaa
      121 agagcctgag tatctcaaca ttgaaattgt tggttcaaca agtgacggcg gagtatattt
      181 tatgcacaat gaaggcttta gtcttgatta ttcaattgaa gaaacaattg gtgactatac
      241 ttatcattac aaccataacg atgatgttat gctttataag aatggcaatg tttactcaat
      301 taaagaggct tatgaaagcg gagttattga cgatagtatt cttgaagaat tgtcaaaaat
      361 gaattttggt ttggaatcta ataaaccgaa tacaactgtt cctactgagc ctacaacttc
      421 ggttgcaaca gttgacgaac ctgtaacagt acccgataca acttctgcaa cttcggcaac
      481 taagcctgtt tcaacaagcg acacagctac aaatgacaca ccgaacaatt cacagaataa
      541 cagcggtgct gtacagacag gacagaacag cgttgctgta attcttcttg ttgcaatgct
      601 tgcaggttgt gcagtaattt ttgcaaaacg cagaaattat tttaaataat tcaaaacatc
      661 aacagaatat ataaaataaa ccgttctcgc ttttgtgaac ggtttattta ttttagctta
      721 aaaatttgtg atagtttatc aagatgtatt gctttttata tcatttaatg ttacaattaa
      781 cttgcataaa aatttagcga aagcttttaa taatgcgtag aatttgaaca aagcgaggga
      841 tagatatgaa aaagttttta cctatagcta tggcggcggt aatgtcgatg tctgcggttg
      901 cggtttcggc agtttctgca aatgcggttg aaccgcttaa cacacccgtg cagtatgcac
      961 aggttgcaag ggcaagcttg gcaacaccga aaatttcaaa agccgaaagc gtttacggcg
     1021 gtgtaaagct tacatggaac aaagtaagcg gtgcaactaa atacagagtt tactacaagg
     1081 gccgtaaagg ttggacacga atggtcgata caacttcaac atcatatatc gacaaggatg
     1141 tttcatctgg caggaattac acctatactg taagatgtat ttctgctgac ggaaagagct
     1201 ttacaagcgg ttatgattca aaggggacaa ctgtaaaata tgttgctgcc cccgaaattt
     1261 caaagcttga aaatgtaaac ggcggtgtga aaattacatg gaacaaagta agcggtgcat
     1321 caaagtatag aatttaccag aaaaattcgt cagattggtt cagagtatca gatacgactt
     1381 ctaattctat agttgataaa tctgttgatg tgggaaccta tacttatacc gtgaggtgta
     1441 taagtgatga cggaaagagt tttgagagtg gatttaaccc aaaaggctta agtatcagat
     1501 ataatcaagc tcctaaaata cttgaaacca atgttgtaaa cggcggtatt aaggtgattt
     1561 gggattctga agaaaataca aattacagat tgtactgcaa aacagaaggt aagggctgga
     1621 caaaagtatg cgatagcaaa acaggtgttg tgacacataa aaatttacag ccgaataaaa
     1681 cctatacata cactgtaaga gcaatcagtt ccgacgctaa aaagtatttg agcggttatg
     1741 accctgtggg aatgagcgca aaatatgttg ccacaccgaa aatttcaaaa actgaggtca
     1801 catatgacgg cgtaaagctg acatggaaca aggttgcagg tgcagagaag taccgtgtgt
     1861 actacaaaga cggtgagggt tggaacaaac ttgccgatac aacgggtaac agttatcttg
     1921 atatgggact tgtatcaaga gaactaagca taaaagataa ttccgtaaac ggtcagtata
     1981 tttatactgt tcgttgcatt tcaagtaacg gcaaggtttt tcagagcggt tatgatacaa
     2041 aaggttctaa ggcgaattta ggcaatgaat gtccggagat tgttcgtgtt gactcaatgc
     2101 atggcggaat tacagtttca tggacagatg tttgcgaaga taattcaaag tatcgtattt
     2161 atgtaaaaga aaaaggttcg tggaaaaagc ttgctgatac aacaaataat tattacactc
     2221 ataaaaatgt aaaagcagga cagacctaca cttataccgt aagatgtgtt tctaaggacg
     2281 gcaagaaatt tacaagtatc tataatccgt caggtgtgac atttaagtat aagactacag
     2341 ataaaacaag agatgcatac atagattatc tgctcaagca tgaatcggaa tggttacctg
     2401 aactcaaggt aggaaatgtt gctttggctg gtgtgcagtt tgccgatctc gattttgacg
     2461 gtgttcccga gcttatagta caggaagcag gagataagga atactatttg aatgcaaagg
     2521 tgtatacctt taaggacgga aagttttcgt tagtcggatt tgatgattat agaacttata
     2581 tcccgaatca actgacttat ttctatgaca aatctgctaa gaaatatata attaatgccg
     2641 ttgaattgtc aacctcaaat gttgaccgtt atcttgaacg cgaaaactat acattgaatt
     2701 atgacggcac agttttggtt gttcgtggat actcttctgc tcatttggct cttgaaagtc
     2761 ggggattgga ataccattat tacaataata ccggaagaac aacaaaacag gcatataatg
     2821 ccacaaatgt gagtgtactc ggtaattgtg taaatgcaca tatgtacagt cagtttgttc
     2881 actgttctgt tgacggctat ccaaaacttt ggaataatta tagtgaaaca cagaagaaac
     2941 agcaccttct tgattcatat aacgcttttt cttacgacaa atattgattg tcaaaattga
     3001 ta
//
LOCUS       DAOMVE010000176         2997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_9692_length_2997_cov_3.716179, whole genome shotgun sequence.
ACCESSION   DAOMVE010000176 DAOMVE010000000
VERSION     DAOMVE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9692_length_2997_cov_3.716179"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..945)
                     /locus_tag="PLS64_08340"
     CDS             complement(115..945)
                     /locus_tag="PLS64_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43866.1"
                     /translation="MNSKVRHIIYGIISFVLSFVLFLLSFAIVLQSTILNPSYIMDNM
                     NTSNYFVDKRDEIKESLVDLGYASGLDEKFFENVVDEVTIHDNTQAYLNSFYAGEEAK
                     IDTTAFKQKFNSELDSYISKNNLKVANDGSREYLINQAANIYAAALRIPLFATLSAYL
                     IALKNMMPLIIGGLAVLVAILCVIIIFTNSWKHRAVRYICYSTSAAFLTVGIIPAVLL
                     STGYMSKINIDSRAFYNLFVQSMNNILIALAICSVIFFIVSIGLFFLHKSMRRHASEE
                     "
     gene            complement(945..1670)
                     /locus_tag="PLS64_08345"
     CDS             complement(945..1670)
                     /locus_tag="PLS64_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006353591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HRL43867.1"
                     /translation="MSDKKIRHGHKSKNGKSFIILPIIFCLITYLVLYLAFVPSFSPV
                     ISAVGMFFSDNEKDYSTEYDNIFVPVSENSTVPTVTKKDSNGQEKTYVKNDSVQYPNY
                     GNLFGELIINDCNVDANLFFGDNDVALRNGVGIYAGSFVPGYGGTILVAGHNNTYFNG
                     LKNAAVGQKVEIKTSYGNYIYEITDTAVKKATDRTAYDLSADYENLIMYTCYPFDALG
                     LTTQRYFVYAKLVSGTPIDKEAN"
     gene            complement(1676..2266)
                     /locus_tag="PLS64_08350"
     CDS             complement(1676..2266)
                     /locus_tag="PLS64_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43868.1"
                     /translation="MKKLSAMLVAFILVAATAFTAFAAGINDSEQAVLNELKTSVKMN
                     GSEMVIPSEFVNQAENYFNTIDMTADESSQIVAILKKGESFLENSGASNIADLTFAQK
                     QTLLSYGKEVVGVLDMTMSYDTSSKKLTIYGKDGKVAFSAVPTLTKAGSVQDNSVIKT
                     TGAEADFSGIIAVSAVAFVMVAGGAFYLAKSKKERA"
     assembly_gap    2771..2780
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          889 a          601 c          532 g          965 t
ORIGIN      
        1 ataaaaacaa aaattgcatc agaacctttt cgggcatcta atgcaattcg tttatctaac
       61 tatcttctcg aacggggaac ggcgacttgc cgtaattttc aactttcaat ttcatcattc
      121 ttcacttgcg tgtctgcgca tactcttgtg caggaagaaa agtccgatcg aaactataaa
      181 aaagattaca gaacagattg caagggcgat aagaatattg ttcatactct gaacaaagag
      241 attgtagaac gcccttgaat caatattaat cttgctcata tagcctgttg aaagcaacac
      301 cgccggaatt ataccaaccg taaggaatgc cgcagatgtt gaataacaga tataccttac
      361 cgctctgtgc ttccaactat ttgtaaagat gatgattacg catagaattg cgacaaggac
      421 tgcaagaccg ccgataatca acggcatcat atttttcaat gcaatgagat aagcagaaag
      481 tgtggcaaaa agcggtattc tgagcgccgc cgcataaatg ttagcagcct gattgatgag
      541 atattctctg ctcccatcgt ttgcaacctt gagattattc ttacttatgt agctgtcaag
      601 ctcgctgtta aatttctgct taaacgctgt tgtgtcaatc ttagcctcct cgcctgcata
      661 aaagctgttg agataggcct gtgtattatc gtgaattgtc acttcgtcaa caacattttc
      721 aaaaaacttt tcatcaagtc ccgacgcata accgaggtca acaaggctct ctttgatttc
      781 gtcacgcttg tcaacaaaat agttgctcgt attcatatta tccataatat acgatgggtt
      841 gaggattgtt gactgaagaa ctattgcaaa cgaaagcaaa aacagtacaa acgagagaac
      901 gaacgaaatt attccgtaaa taatatgtct tactttagaa ttcatcagtt cgcctcctta
      961 tcaatcggag tacccgacac taactttgca tacacaaaat atctctgtgt tgtaagaccg
     1021 agtgcatcaa acggatagca ggtgtacatt atgagatttt cataatccgc cgaaaggtca
     1081 taagcggttc tgtcagtcgc ttttttgaca gccgtgtcgg tgatttcata gatgtaatta
     1141 ccgtagcttg tcttgatttc aaccttctga ccgactgctg cgtttttaag accgttgaaa
     1201 taagtattgt tatgacctgc aacgaggatt gttccgccgt aaccggggac aaaacttccg
     1261 gcataaatgc caacaccgtt tctgagtgca acatcattgt caccgaaaaa aagatttgcg
     1321 tcaacattgc agtcattgat tataagctca ccgaacagat ttccgtagtt agggtactga
     1381 acggagtcgt ttttaacata agttttttcc tgaccgtttg aatccttttt tgtaacggta
     1441 ggaacggttg agttttctga cacgggcaca aaaatattat cgtactctgt tgaataatcc
     1501 ttttcattat ctgagaagaa catacctact gccgaaatca caggtgaaaa ggacggaaca
     1561 aaagcaagat acagcacaag atatgttatc aaacaaaaga taatgggaag aattataaaa
     1621 ctcttcccat tctttgattt atgaccgtgt ctgatttttt tatcagacat tagaatcaag
     1681 ctctttcttt tttagatttt gcaaggtaga aagcaccgcc tgctaccatt acaaatgcaa
     1741 cagcagaaac agcgataatg ccgctgaagt cagcctctgc gcctgttgtc ttgataacac
     1801 tgttatcctg aacactgcct gcctttgtaa gtgtaggaac agctgagaat gcaaccttgc
     1861 cgtctttacc gtaaattgta agcttctttg aagatgtgtc atatgacatt gtcatatcaa
     1921 gcacaccgac aacttcctta ccgtaagaaa gaagtgtctg cttctgagca aatgtgagat
     1981 ctgcaatatt agatgcacct gagttctcaa ggaagctctc gcccttcttg agaatagcaa
     2041 cgatctgaga tgactcatct gctgtcatat cgattgtgtt aaagtagttt tcagcctggt
     2101 ttacaaactc gctcggaata accatctccg aaccgttcat cttaacagat gtcttgagtt
     2161 cgttgagaac agcctgttcg ctgtcattga ttcctgctgc aaaagcagtg aaagcagttg
     2221 ctgcaacaag gatgaacgct actaacattg ctgataactt tttcatagtt cataccacct
     2281 tgtattttat tttgttttca tcagcaagaa gcttcacgga ttcattggca taatacgcat
     2341 ctgcaagccg aaaactgcag ggagcacaat ggaaaacttc accatttaac ttctttgtac
     2401 agtattatac accacatttc gcattttgta aagcatttta ttataaaaat ttgttatatt
     2461 cggaaaaatt acaatccttt tgtcaaatca ggcattttca ttgtgcatta tgccgtttta
     2521 aatcaatttt gcgctgcatc gttttcttct aatcttgtat tttaattcat ttgtacactt
     2581 gccttttgca taaaaacacg cctgccgatt tataaattcc ttcttttgca aatattcgtg
     2641 tacagacctt atccgcttac cctacacctt tttctgtcat tgttttctta ttatgacata
     2701 ttgtgacata ctaatatata agtagaaaat ttatcggtta tttgccatat ttttgtttat
     2761 tttttttgca nnnnnnnnnn tatttttcgg ctaactgcaa caaaataatc atatatttta
     2821 tgtatgtttt gtacacatga aaaatcagag taattgcata ttttgcaaaa aagacatatt
     2881 tttcctttat taaacctttg ttcatattga tttttttact gatttaaaat ataataggag
     2941 tgtcatgttt aaaagtattt tgtgcttttg tgccgttttt cgcctacagc actttcg
//
LOCUS       DAOMVE010000177         2910 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10097_length_2910_cov_3.357268, whole genome shotgun sequence.
ACCESSION   DAOMVE010000177 DAOMVE010000000
VERSION     DAOMVE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10097_length_2910_cov_3.357268"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1084
                     /locus_tag="PLS64_08355"
     CDS             <1..1084
                     /locus_tag="PLS64_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRL43869.1"
                     /translation="SGIDTKQAQTIEKMPEIENYLTVGSQYGCVSADVPVNECGENFL
                     YDNADGTKSFSVEFLEFEHDTFVQICRELKLDYNKVKGGVLAYSKVTPDNDEDSRSYD
                     EPVPLYGKDAPTKFIVYGNDDNGNELIAGKLKVSSVFDEIPKSADSVIGEGTIFGQGL
                     IIGEQGVISSQVGKNGCAVTLYANTSDHTSLTNRIESMSGTGDSESYISIFDYEEIVR
                     QFNAVMLIVGIFVYGFIGVISLIGLTNIFNTISTNMQLRSKEFASLKSIGMTKKEFNR
                     MIRLESLMYGIKSLIIGIPLGVLGVFAIFSAFSRGNVPMSFVFPWKAILISIAAVIIV
                     VWLIMKYSISKVNKQNIIETIRNDNI"
     gene            1255..1902
                     /locus_tag="PLS64_08360"
     CDS             1255..1902
                     /locus_tag="PLS64_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRL43870.1"
                     /translation="MKILLIEDNRAISKGLVYTLEQNGFEVALCENAESTLNSLGGDF
                     DLFIVDVSLPDGNGFELCDEIKRVCETPVIFLTALDDEDSIVKGFDLGAEDYITKPFS
                     SRELLARIKRITKKLDKTMAMNFGDISIDFDKKQVCKNGNPVVLTALEYRLFAMLAKN
                     SGKVLTREQILERIWDLSGNYVEDNTLTVYIKRLRKKLDTNMIKTVKGLGYRLEV"
     gene            1902..>2910
                     /locus_tag="PLS64_08365"
     CDS             1902..>2910
                     /locus_tag="PLS64_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRL43871.1"
                     /translation="MKNTSKFLAVMLAITGLFCCAVAVGIGAGFNAVRESTDKKIIAV
                     AEKVREQYPDISEKELAQILNSDTAGIKGDFAKYGIKSDSGWVDIQSGSYATITIVIA
                     TAGVCVFGILLCVVFIRYCKKQKRQRLEIVKCLEKINNREYDLDLDGSTDDDMSLLKQ
                     EIQKTTVMLREYADNSMREKEILKNSLADISHQLKTPLTSILITTENILDDDDMPVEI
                     RRDFVMDIAHNAHSINFLVKSLLTLSMLDSGTVELKFGDVSVEKIIDECISRTEVLAD
                     IRDVRIEKTVKNDFYLNCDFRWICEAVSNIVKNCIEHTDGGYVKISAEKNSMYSEITI
                     SD"
BASE COUNT          885 a          538 c          655 g          832 t
ORIGIN      
        1 ttccggaatt gatacaaagc aggctcagac aattgaaaaa atgcctgaaa ttgagaatta
       61 tcttacagtc ggatcgcagt acggttgtgt ttctgcggat gtgcctgtta acgaatgcgg
      121 agaaaatttc ctttatgaca atgctgacgg tacaaagtca ttcagcgtgg aatttcttga
      181 gtttgaacac gatacctttg ttcagatttg cagggaatta aaacttgatt acaacaaggt
      241 taagggcggt gtacttgcgt attcaaaggt gacgcccgat aacgacgagg acagccggag
      301 ttatgatgag cctgtaccgc tgtacggcaa agacgccccg acaaagttta tcgtttacgg
      361 aaatgatgat aacggaaatg agctgatagc aggcaaatta aaggtttcgt cagtgtttga
      421 tgaaattcca aagtcggctg attctgtaat cggcgaagga acaattttcg gtcagggatt
      481 gataatcggt gaacagggtg taatttcatc tcaggtcggc aaaaacggat gtgccgttac
      541 tctctatgcc aatacatcag atcacacttc gcttacaaat cgtattgagt ctatgtcggg
      601 tacaggtgac tcggaaagct atatttctat ttttgactat gaagaaattg taagacagtt
      661 caatgccgtt atgctgattg tcggaatatt tgtttacggt ttcatcggtg ttatctcgct
      721 tatcggactt acaaacattt tcaacacaat ttcaaccaat atgcagttga ggagcaagga
      781 atttgcatcg cttaaatcta tcggtatgac aaagaaagaa tttaaccgta tgattcgcct
      841 tgaaagcctt atgtacggca taaaatcttt gattatcggt attcctctcg gcgttctcgg
      901 tgtatttgca atcttctcgg cattttcaag aggcaatgtt ccaatgagct ttgtattccc
      961 gtggaaagct atattgatta gtattgcggc tgtaattatt gtagtatggc tgattatgaa
     1021 gtactcgatc tcaaaggtta ataagcaaaa tattattgaa acaatcagaa atgacaatat
     1081 ctgagcgtca tattcagcca acagactgta ttttgttgaa atttgttgac aatagtttta
     1141 cactctgtta taatgtagtt gtaacaacag ataccacaat attttgtatg ttttcattgc
     1201 tgacgggcgg attattgatt taatccgccc gaatttcatt ttggaggact tgttgtgaaa
     1261 attcttctta ttgaggacaa ccgtgcaatt tcaaaaggac ttgtttacac tcttgaacaa
     1321 aacggctttg aggttgcgtt gtgcgaaaat gccgaatcaa cccttaactc tctcggcggt
     1381 gactttgacc tttttattgt tgatgtgtcg cttcccgacg gcaacggttt tgaactttgt
     1441 gacgagataa aaagagtgtg cgaaactccc gtgatttttc tcacagcact tgacgatgag
     1501 gacagcattg taaaaggctt tgacctcgga gcggaggact acataacaaa accgttctca
     1561 agccgtgagc ttcttgcaag aattaaacga attaccaaaa agctcgacaa aactatggct
     1621 atgaattttg gcgacatcag cattgacttt gacaaaaaac aggtttgcaa aaacggcaat
     1681 cccgttgtgc ttacggcgct tgagtaccgc ctgtttgcaa tgcttgccaa aaacagcggc
     1741 aaggtgctga cccgtgaaca gattcttgaa cgcatatggg atttgtcggg gaattatgtt
     1801 gaggacaaca cgctgaccgt gtacataaaa agacttcgca aaaaacttga tacaaatatg
     1861 attaaaaccg taaagggcct tggttacagg ctggaggtat aatgaaaaac acatcaaaat
     1921 ttcttgcggt aatgcttgcg ataacgggac ttttctgttg tgccgttgca gtaggcatcg
     1981 gtgcaggctt taacgctgtt cgtgaaagca cggataaaaa gataattgcc gttgcggaaa
     2041 aggttagaga acaatatccc gacatttcgg aaaaggagct tgctcaaatt ttgaacagcg
     2101 acactgccgg cataaaaggc gactttgcaa agtacggaat aaaatccgac agcggctggg
     2161 ttgacattca gagcggaagt tatgcaacga ttacaattgt gattgcaacc gcaggtgttt
     2221 gtgtgttcgg aattttgctc tgcgttgtgt tcataagata ctgtaaaaag cagaagcgac
     2281 agcgacttga aattgtaaaa tgtcttgaaa aaataaacaa ccgagagtac gacctcgacc
     2341 ttgacggctc aaccgacgac gatatgtcgc tgttaaagca ggaaattcaa aaaacaacgg
     2401 taatgcttcg tgagtatgcc gacaattcaa tgcgagagaa agaaatcctc aaaaattcgc
     2461 ttgccgacat ttcacatcag cttaaaacac cgctgacttc aattttgatt acaaccgaaa
     2521 atatccttga tgacgatgat atgcccgttg aaatcagacg ggattttgtt atggatatag
     2581 cgcataatgc ccattcgata aatttccttg taaaaagtct gctcacgctc tcaatgcttg
     2641 attcgggaac tgttgaattg aagtttggag atgtgtcggt tgaaaaaata attgacgagt
     2701 gcatttcacg cacagaggtt cttgccgata tccgtgatgt gagaattgaa aaaaccgtca
     2761 aaaatgattt ttatcttaat tgcgattttc gctggatttg cgaagcggtt tcaaacattg
     2821 taaaaaactg tattgagcat accgacggcg gttatgtcaa aatttcagcc gaaaagaaca
     2881 gtatgtattc ggaaatcacc ataagcgaca
//
LOCUS       DAOMVE010000178         2874 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10270_length_2874_cov_2.196878, whole genome shotgun sequence.
ACCESSION   DAOMVE010000178 DAOMVE010000000
VERSION     DAOMVE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10270_length_2874_cov_2.196878"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..508
                     /locus_tag="PLS64_08370"
     CDS             107..508
                     /locus_tag="PLS64_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009445760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HRL43872.1"
                     /translation="MRYMLDTNICIYSIKHKPKQVFLKLQEHDPEDICISSVTYAELV
                     HGVEKSQAIEKNQLALAILLANIEVLDFDSAAAESYGKIRAYLEKQGTPIGSLDTMIA
                     GHAKSLDYVLVTNNTKGFSRVQDLKLENWAE"
     gene            551..805
                     /locus_tag="PLS64_08375"
     CDS             551..805
                     /locus_tag="PLS64_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43873.1"
                     /translation="MSTNKITCSFTVDRDIYNAYKSIVVKGGENVKGNLIRYMLNVIN
                     YETPNADTISAIKEVEELKKNPNKKTYASFRELLEDITNE"
     gene            798..1076
                     /locus_tag="PLS64_08380"
                     /pseudo
     CDS             798..1076
                     /locus_tag="PLS64_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
     gene            complement(1168..>2874)
                     /locus_tag="PLS64_08385"
     CDS             complement(1168..>2874)
                     /locus_tag="PLS64_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRL43874.1"
                     /translation="GSQFDTSEPSSLTETTSAYGTEASTESTKMTTENMEFTETTISD
                     FSSACTEVTTEYTETTAYTETTTECVEETTIVDEPSTESTETTTEEVTEPSTEEYTEQ
                     ITEQPTTEPKPTVPPKSVKFNKNGITLGVGESYTLITTIENGDISQVEFTTDNSGVIT
                     VDDKGKMTAVGIGVSTITAKTYNGLTAKCKVTVKKLANSIKLDKTSIILGVGEQYDFS
                     SYVPSGTAAYYRSYYSDDPNIAFVQKAGGLMTAKKAGTTTMRCKMPNGTQATCNVTVK
                     PLATSLKLNASEIVLYIGQSFDINSSVPKGTAAYYRLYSSSNSKIAAVTRGGGVVKGV
                     ATGKATVTCTLNNGKKAICNVYIMPQSKKISNVPLIGQSKLPTGCETCSATMLLNFYG
                     YKISETTFADKYLVKKPFGYSNGSYTGPDPNCAFVGTPYSSNSYGAYAPIMVKCMNKY
                     LSDKSYKAVEISGKSLEYLSGKYVAQGQPIMVWATINMSPSFKTTTWRVNYTDENAKY
                     KLGSYYTWTAGEHCLLLTGYDKDYYYFNDPWTNARTRYSKSLVNARYNELGKQAVVMV
                     KK"
BASE COUNT          859 a          528 c          531 g          956 t
ORIGIN      
        1 ctatgaccaa tcaatggagt tctttcattc aagcagtaaa tatgttttct gatgattttt
       61 ttgaaaacgg cagagaagat tcagaaccac aggagcgtga aaaactatga gatacatgct
      121 tgatactaat atttgcattt attctattaa gcataaacca aaacaggttt ttcttaaatt
      181 acaggaacat gatcctgaag atatatgtat ctcatcggta acatatgctg agttagtaca
      241 cggagttgaa aaaagccaag ctattgaaaa aaaccaatta gctttagcta tattgctggc
      301 gaatattgaa gttcttgatt ttgattcagc cgctgcggaa tcttacggga aaatacgagc
      361 ctatttagaa aagcaaggta cacccatcgg ttcacttgac acaatgattg caggacatgc
      421 aaaatcatta gattatgtgt tggttactaa taacacaaaa ggattttcga gagtacaaga
      481 tttaaaactt gaaaactggg ctgaatgatt taacaataat agtcatattt ttttagaagg
      541 tgataaaatt atgagtacca ataagattac ttgttcattt accgttgacc gtgatattta
      601 caacgcttat aaaagtattg ttgtaaaagg tggtgaaaat gtaaagggca accttatccg
      661 ttatatgctc aatgtcatta actatgaaac tccaaatgca gatacaattt ctgcaataaa
      721 agaagttgaa gaactgaaaa aaaatccaaa caaaaaaacc tatgcttcat ttagagagct
      781 tttagaggat attaccaatg agtaaattaa gaattgtgcc atcacagaaa ttcaacagag
      841 atgttaagct ggcaattaag cgtggataca atattcaatt gcttgatgat gttgttgaaa
      901 tgatagctga acaacaacta ctgccaccgg aatataaaga ttacaaacta attggaaatt
      961 attccggctg tagggagtgt cacattaccc ccgactggct cctgatttaa gaaattagta
     1021 atgaagaact tattttgtat ctgacaagga ccggaacaca cagcgattta ttttaaactt
     1081 ctaaaggcgt ttttgcctta atagaatata ccaacaaata aaagaaagaa ccttccctaa
     1141 tgataaattc atcagagaag gttcttgtta ttttttcacc ataactacag cctgtttgcc
     1201 gagttcatta tatcttgcat ttacaaggct ttttgagtat cttgtacgag cgtttgtcca
     1261 tgggtcgttg aaatagtaat aatctttgtc atagcccgtg agcaacaaac aatgttcgcc
     1321 ggcagtccaa gtgtagtaac tgccgagttt atattttgcg ttttcatcgg tgtagtttac
     1381 tctccaagtg gttgtcttaa atgacggtga catattaatt gttgcccaca ccataatcgg
     1441 ctgaccttgt gcaacatact tacccgaaag atattcaaga cttttcccgc tgatttctac
     1501 agccttgtat gatttgtcgg aaaggtattt attcatacat tttaccataa tcggtgcata
     1561 cgctccgtag ctgttggaac tgtatggtgt tccaacaaag gcacaattcg gatcaggtcc
     1621 tgtgtatgaa ccgtttgaat aaccgaaagg tttttttacc aaatacttat ctgcaaatgt
     1681 cgtttcagaa attttatatc cgtagaaatt caacagcatt gttgcactac aggtttcaca
     1741 gcctgtcggg agcttacttt gaccgattaa cggaacattt gaaattttct tgctctgtgg
     1801 cattatgtat acattgcaaa tggctttttt tccgttattc aaagtgcaag ttacagttgc
     1861 ttttcctgtc gctactcctt tgacgacacc tccgcctctt gttacagcag ctatttttga
     1921 attgcttgat gaataaagtc tgtaatatgc cgctgtacct tttggaacac tgctgttaat
     1981 gtcaaaactc tgacctatgt acagtacaat ttcacttgcg ttaagtttaa gagatgttgc
     2041 aagcggcttg accgttacat tacaggttgc ttgtgtaccg tttggcattt tgcatctcat
     2101 agttgtggtt cctgcctttt ttgcggtcat caaacctccg gctttttgaa caaaagcaat
     2161 attcggatca tctgaataat aagaacgata ataagctgct gttccgctcg gcacatagct
     2221 tgaaaaatca tactgttcac cgactccaag tataatactt gtcttatcaa gctttatgct
     2281 attagcaagc tttttaacag taaccttaca tttggctgta agtccgttat aagtttttgc
     2341 tgtaattgta gagacaccaa tgcccacagc agtcattttg cctttatcgt ctacagtaat
     2401 taccccacta ttatctgtag taaactctac ttgcgaaata tctccatttt caatagtagt
     2461 aataagagta tatgactccc ctaccccaag tgttattcca ttcttattga atttcacact
     2521 tttaggcgga acagttggtt ttggctctgt agtaggctgt tcagtaatct gctcagtata
     2581 ctcttctgtt gaaggttcag taacttcttc cgtagttgtt tcagtcgatt ctgtcgatgg
     2641 ttcatcaaca attgttgttt cctcaacaca ttctgtagta gtttcagtat atgctgtagt
     2701 ttcagtatat tcagttgtta cttcagtaca agcagaggag aaatccgata tcgttgtttc
     2761 tgtgaattcc atattttctg ttgtcatctt tgtactttct gtggatgctt cagttccgta
     2821 agccgaggta gtttctgtca aagatgacgg ttctgatgta tcgaattgag atcc
//
LOCUS       DAOMVE010000179         2866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10299_length_2866_cov_3.837780, whole genome shotgun sequence.
ACCESSION   DAOMVE010000179 DAOMVE010000000
VERSION     DAOMVE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10299_length_2866_cov_3.837780"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..678)
                     /gene="rpsC"
                     /locus_tag="PLS64_08390"
     CDS             complement(7..678)
                     /gene="rpsC"
                     /locus_tag="PLS64_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRL43875.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFANKKDFGATLVEDYNLRKTLK
                     AQLYNFGISKIEIERDGKKVKISIHCAKPGLVIGKGGSEIEKLRAQCEKMLSKPVAIN
                     IVEVKAPELDAQLVAESIAQQLEKRISFRRAMKMSIGNAMRRGAKGIKIMCSGRLGGA
                     EIARSEQYHEGTIPLQTLRADIDYGFAEANTTYGKVGVKVWLYKGEVLNEVKSRKPRR
                     EGGRK"
     gene            complement(693..1034)
                     /gene="rplV"
                     /locus_tag="PLS64_08395"
     CDS             complement(693..1034)
                     /gene="rplV"
                     /locus_tag="PLS64_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRL43876.1"
                     /translation="MEAKANAKFVRISPRKVNVVLDLVRGKDVKYAEAVLKHTPKAAC
                     EPLLKLLNSAMANAENNHDMDVSKLYIAQCNATAGPILKRIRPRAQGRAFRINKRTSH
                     ISLVLKEREDD"
     gene            complement(1055..1327)
                     /gene="rpsS"
                     /locus_tag="PLS64_08400"
     CDS             complement(1055..1327)
                     /gene="rpsS"
                     /locus_tag="PLS64_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRL43877.1"
                     /translation="MSRSTKKGPYVQPVLLKRVIEMNEAGEKRILKTWSRSSTIFPEF
                     VGHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHAGSKTSK"
     gene            complement(1347..1442)
                     /gene="rplB"
                     /locus_tag="PLS64_08405"
                     /pseudo
     CDS             complement(1347..1442)
                     /gene="rplB"
                     /locus_tag="PLS64_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966409.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            1425..1520
                     /gene="rplB"
                     /locus_tag="PLS64_08410"
                     /pseudo
     CDS             1425..1520
                     /gene="rplB"
                     /locus_tag="PLS64_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966409.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
     gene            1540..1812
                     /gene="rpsS"
                     /locus_tag="PLS64_08415"
     CDS             1540..1812
                     /gene="rpsS"
                     /locus_tag="PLS64_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRL43878.1"
                     /translation="MSRSTKKGPYVQPVLLKRVIEMNEAGEKRILKTWSRSSTIFPEF
                     VGHTFAVHDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHAGSKTSK"
     gene            1833..2174
                     /gene="rplV"
                     /locus_tag="PLS64_08420"
     CDS             1833..2174
                     /gene="rplV"
                     /locus_tag="PLS64_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007047291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRL43879.1"
                     /translation="MEAKANAKFVRISPRKVNVVLDLVRGKDVKYAEAVLKHTPKAAC
                     EPLLKLLNSAMANAENNHDMDVSKLYIAQCNATAGPILKRIRPRAQGRAFRINKRTSH
                     ISLVLKEREDD"
     gene            2189..2860
                     /gene="rpsC"
                     /locus_tag="PLS64_08425"
     CDS             2189..2860
                     /gene="rpsC"
                     /locus_tag="PLS64_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRL43880.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFANKKDFGATLVEDYNLRKTLK
                     AQLYNFGISKIEIERDGKKVKISIHCAKPGLVIGKGGSEIEKLRAQCEKMLSKPVAIN
                     IVEVKAPELDAQLVAESIAQQLEKRISFRRAMKMSIGNAMRRGAKGIKIMCSGRLGGA
                     EIARSEQYHEGTIPLQTLRADIDYGFAEANTTYGKVGVKVWLYKGEVLNEVKSRKPRR
                     EGGRK"
BASE COUNT          770 a          663 c          663 g          770 t
ORIGIN      
        1 ataacattac tttctgcctc cttctctgcg tggctttctt gacttaactt cgttaagaac
       61 ctcgcccttg taaagccaaa ccttaacgcc tactttaccg taggttgtgt tagcttctgc
      121 aaagccgtag tcgatgtcgg cacgaagtgt ctgaagcgga atagtacctt cgtggtactg
      181 ctctgaacga gcaatttcag caccgccaag acggcctgaa cacataatct tgataccctt
      241 agcgccacga cgcattgcgt tgccgattga catcttcatt gcacggcgga aagagattct
      301 cttttcaagc tgctgagcga tgctttctgc aacgagctgt gcatcaagct caggagcctt
      361 aacttctacg atattgattg caacgggctt tgagagcatc ttttcgcact gtgctctgag
      421 cttttcaatc tcagaaccgc ccttaccgat tacaagaccg ggcttagcgc agtggatgct
      481 gattttaacc tttttgccgt cacgctcaat ttcaatcttt gagataccga agttgtaaag
      541 ctgagccttc agagtcttac ggaggttata gtcctcaacg agtgtagcac cgaagtcctt
      601 cttgtttgca aaccaacggg aatcccaatc tttaataacg ccgacacgga gaccgtgcgg
      661 attaactttc tgacccataa tctaacctcc ccttagtcgt cttctctttc cttgagtacg
      721 agggaaatgt gagaagttct cttgttgatt ctgaacgctc tgccctgtgc tctcggacga
      781 attcttttaa ggatggggcc tgctgttgca ttgcactgag caatgtaaag cttggataca
      841 tccatgtcat ggttgttctc agcatttgcc atagctgaat tgagcagctt taaaagcggc
      901 tcgcaagcgg ccttgggagt gtgcttaaga acagcttccg catacttaac gtctttgcct
      961 cttacgaggt caagaactac attaaccttg cggggtgaaa tacgaacaaa ttttgcattt
     1021 gcctttgctt ccatctgcaa tactctcctt ccgcttattt gcttgtcttt gatcctgcgt
     1081 gacctttgaa tgttcttgtc ggagcaaact caccgagctt gtgaccaacc atatcttcgg
     1141 ttacatatac aggaacatgt tttctgccgt catgaacggc aaatgtgtga ccaacgaatt
     1201 cagggaaaat tgttgaactt cttgaccaag tcttgagaat tctcttttcg cctgcttcgt
     1261 tcatttcgat aacccttttg agcagtacag gctgaacata agggcccttt ttagtacttc
     1321 tactcatgtt ttagctcctt tccgccttac ttaccgtttc ttcttcttac gataagcttg
     1381 tcgctgctct tgttgttctt acgagtcttg tagccgagtg cgggcttacc ccatggggta
     1441 agcccgcact cggctacaag actcgtaaga acaacaagag cagcgacaag cttatcgtaa
     1501 gaagaagaaa cggtaagtaa ggcggaaagg agctaaaaca tgagtagaag tactaaaaag
     1561 ggcccttatg ttcagcctgt actgctcaaa agggttatcg aaatgaacga agcaggcgaa
     1621 aagagaattc tcaagacttg gtcaagaagt tcaacaattt tccctgaatt cgttggtcac
     1681 acatttgccg ttcatgacgg cagaaaacat gttcctgtat atgtaaccga agatatggtt
     1741 ggtcacaagc tcggtgagtt tgctccgaca agaacattca aaggtcacgc aggatcaaag
     1801 acaagcaaat aagcggaagg agagtattgc agatggaagc aaaggcaaat gcaaaatttg
     1861 ttcgtatttc accccgcaag gttaatgtag ttcttgacct cgtaagaggc aaagacgtta
     1921 agtatgcgga agctgttctt aagcacactc ccaaggccgc ttgcgagccg cttttaaagc
     1981 tgctcaattc agctatggca aatgctgaga acaaccatga catggatgta tccaagcttt
     2041 acattgctca gtgcaatgca acagcaggcc ccatccttaa aagaattcgt ccgagagcac
     2101 agggcagagc gttcagaatc aacaagagaa cttctcacat ttccctcgta ctcaaggaaa
     2161 gagaagacga ctaaggggag gttagattat gggtcagaaa gttaatccgc acggtctccg
     2221 tgtcggcgtt attaaagatt gggattcccg ttggtttgca aacaagaagg acttcggtgc
     2281 tacactcgtt gaggactata acctccgtaa gactctgaag gctcagcttt acaacttcgg
     2341 tatctcaaag attgaaattg agcgtgacgg caaaaaggtt aaaatcagca tccactgcgc
     2401 taagcccggt cttgtaatcg gtaagggcgg ttctgagatt gaaaagctca gagcacagtg
     2461 cgaaaagatg ctctcaaagc ccgttgcaat caatatcgta gaagttaagg ctcctgagct
     2521 tgatgcacag ctcgttgcag aaagcatcgc tcagcagctt gaaaagagaa tctctttccg
     2581 ccgtgcaatg aagatgtcaa tcggcaacgc aatgcgtcgt ggcgctaagg gtatcaagat
     2641 tatgtgttca ggccgtcttg gcggtgctga aattgctcgt tcagagcagt accacgaagg
     2701 tactattccg cttcagacac ttcgtgccga catcgactac ggctttgcag aagctaacac
     2761 aacctacggt aaagtaggcg ttaaggtttg gctttacaag ggcgaggttc ttaacgaagt
     2821 taagtcaaga aagccacgca gagaaggagg cagaaagtaa tgttat
//
LOCUS       DAOMVE010000180         2864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10310_length_2864_cov_2.547170, whole genome shotgun sequence.
ACCESSION   DAOMVE010000180 DAOMVE010000000
VERSION     DAOMVE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10310_length_2864_cov_2.547170"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLS64_08430"
     CDS             <1..609
                     /locus_tag="PLS64_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HRL43881.1"
                     /translation="SGGITDTVRFRKFLVGCAALVSEQVNYSTLAESADIAPSTAKEW
                     LKVLVGLHIVYLLAPYSNNELKRLSKTPKLYFCDTGLCAYLSMWLTADTLRNGAASGH
                     YYENYVVMELVKNYAYAKSKVNITYFRDSNAKEIDVFIEENNVIHPLEIKKSASPDRR
                     EVKKYSVIDKASLNRGNGGIICMCEEPIPIDKDNCFIPSNLI"
     gene            complement(775..2496)
                     /locus_tag="PLS64_08435"
     CDS             complement(775..2496)
                     /locus_tag="PLS64_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43882.1"
                     /translation="MKDKILNALKSKTFFALAINIVIMALIIGVTAFSYDSADDFYNS
                     LYICQYHNYYNNDINYIFATITGSLQYILLNFNCFVLFQILLSCAAFSSVTFVFADKF
                     GKHKAFIFTLVLNILFSFDHYSNILSSKTAALLLTAGFLMALNAIRNKRYSLPFWIGV
                     LEVIFGTFLCFKYFFVGLAFFITFFIGDMIAKRKYKLPFRKFFWYFRPFVLVFVFIVL
                     VGCGLEYYSYSVNNANAETSGLYRYSVLADKTDDNAFPNYSDYCEELNSVGINSANEY
                     ELFVDGYYDENNGLNTAALEKVADIQKRENPYNFFDNAGALFSDAWGHIINFDSYLIA
                     FAVILAVMVVFVTVQKKRFAFFPALYFAVGFAACMYVRYAMDSSAYTMYGILLMILSF
                     TMYSFDFGHLRKDEYSNITDKSRKTVLISVIVLVILGVASCVSYFFHITPVSYDKKPS
                     DLYTEVDRHPENYYVLDPVTAKEYISYTDNYIHPLWGFSSDFLHNVDGFGYLHRTNQL
                     VNRNLSTNIYEAVVDGRNVYVIDKNITFIKENYLNQYYGKKDSVIVYKDEKEFNGFTI
                     YSVDRVK"
     gene            complement(2532..>2864)
                     /locus_tag="PLS64_08440"
     CDS             complement(2532..>2864)
                     /locus_tag="PLS64_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YARHG domain-containing protein"
                     /protein_id="HRL43883.1"
                     /translation="NSNSQSSNAQSSSNTNTNNNGGFIFPNSDSSYLSNAQVSALSDD
                     DLQLAINEIYARRGRIFKDASLNAYFNSQSWYEGKYTPEEFEKNVKFNTYEQKNLQLL
                     INERRSRK"
BASE COUNT          956 a          544 c          515 g          849 t
ORIGIN      
        1 tccggcggta ttaccgacac agttcgtttt cgtaagtttc ttgtcggttg tgctgctctt
       61 gtttcagagc aggtaaatta ttctactctt gcggaatccg ctgacattgc accaagtacc
      121 gcaaaggaat ggctgaaagt acttgtggga cttcacattg tttatctgct tgccccatat
      181 tcaaacaatg aactgaaacg gctcagcaaa acaccgaaac tgtatttctg tgacaccggt
      241 ctttgtgctt acctttcgat gtggctgacg gcagacactc tccgtaacgg tgctgcaagc
      301 ggtcattact acgaaaatta tgtggtaatg gagcttgtga aaaactacgc ttacgctaaa
      361 tctaaagtaa atattaccta ttttcgtgat tctaacgcaa aagagattga tgttttcatc
      421 gaggaaaaca atgtcattca tccgttggaa atcaaaaaga gtgcttctcc tgatcgtcgt
      481 gaggtcaaaa agtacagcgt aattgacaag gcttctttga accgcggaaa cggcggcatc
      541 atatgtatgt gtgaggagcc gataccgatt gacaaggata attgttttat tccgagcaat
      601 ctaatctgaa tcactcccat agcaaaagta tattttcaag aaactaagca gagcccaaaa
      661 gctctgcttt ttgttttagt ttgcccggaa aataacagaa aaggaatgaa cgaaatgcct
      721 aagaaaaacg acttaaaccg tctccccaaa aatcgtgata tacctttttt aatgttattt
      781 aactctgtct acgctgtaaa ttgtaaatcc gttgaattcc ttttcatcct tgtaaacaat
      841 gacggaatcc tttttgccgt aatattggtt aagataattt tctttgataa aagtaatgtt
      901 tttatcgata acatatacat ttcttccgtc aacgaccgcc tcataaatat ttgttgagag
      961 gttgcggttc acaagctgat ttgtccgatg aagatatccg aagccgtcaa cattatggag
     1021 aaaatcggaa gaaaatcccc acagcggatg aatatagtta tcggtatatg aaatgtattc
     1081 tttagctgtt acaggatcga gaacataata attttcggga tgtctgtcaa cctcagtata
     1141 aagatcactc ggctttttgt catatgaaac aggagtgata tggaagaaat atgatacaca
     1201 gcttgccaca cctaatatca caagtacaat aaccgaaata agaacagttt ttctgctttt
     1261 atctgtaata ttgctgtatt cgtcttttct gagatgaccg aaatcaaacg aatacattgt
     1321 aaacgaaaga atcatcagca aaattccgta cattgtgtat gccgaagagt ccattgcata
     1381 acgaacatac atacaagccg caaatcccac cgcaaaatac aaagcaggga agaaggcaaa
     1441 tctttttttc tgaacggtaa caaaaacaac cataacggca agtattaccg caaaagcaat
     1501 aagatatgaa tcaaaattta taatatgtcc ccatgcatct gaaaacaatg cacccgcatt
     1561 atcaaagaaa ttatacggat tttcccgttt ttggatatcc gcaacctttt caagggcagc
     1621 agtattcagt ccattgtttt catcataata tccgtcaaca aaaagttcat actcatttgc
     1681 cgaatttatt ccgaccgaat taagctcctc acagtaatcg gaatagtttg gaaatgcatt
     1741 atcatcggtc ttatcggcaa ggacgctgta tctgtacagc cccgaagttt ctgcatttgc
     1801 attattgacc gaatatgaat aatattcaag accgcagccg acaagaacaa taaagacaaa
     1861 tacaagcaca aaaggtctga aataccagaa gaattttctg aacggcaact tgtactttct
     1921 ttttgcaatc atatcaccga tgaagaatgt aataaaaaat gcaagtccca caaagaaata
     1981 tttgaaacat aaaaatgttc cgaatataac ttcaagtaca ccaatccaaa acgggagaga
     2041 gtacctttta tttctgattg cattaagtgc catcagaaac cctgccgtaa gcagcaaagc
     2101 ggcggtttta ctgcttaaaa tgtttgaata atggtcaaat gaaaacaaaa tattcagcac
     2161 aagagtgaat atgaaagcct tgtgctttcc gaatttatcg gcaaaaacaa atgtaacaga
     2221 agaaaaagcg gcacaggaga gaagaatttg aaaaagtaca aaacagttga aattcaaaag
     2281 aatgtactgc aatgaaccgg taattgtcgc aaaaatgtag ttaatatcat tattgtaata
     2341 gttatgatac tgacagatat ataaagaatt gtaaaaatcg tcagcactgt cataggaaaa
     2401 tgcagtaaca cctataatca aagccataat aacaatatta attgccaaag caaagaaggt
     2461 cttgcttttt agtgcgttta atattttatc tttcataata actcctctgc attatctttc
     2521 ttgtttaaaa atcactttct tgatcttctt tcgttaatca aaagctgaag attcttctgt
     2581 tcgtaagtat taaatttaac attcttctca aattcttcgg gtgtatattt gccctcatac
     2641 catgactgcg agttaaagta tgcattaagg cttgcgtcct taaaaattct gcctcgtctt
     2701 gcataaatct cattaattgc aagctgtaag tcgtcatcac tcaaagcgct tacctgtgcg
     2761 ttgctgagat atgaagaatc gctgttaggg aagataaatc cgccgttatt attagtgttt
     2821 gtattgcttg atgactgtgc atttgaactc tgtgaatttg aatt
//
LOCUS       DAOMVE010000181         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10341_length_2857_cov_1.747323, whole genome shotgun sequence.
ACCESSION   DAOMVE010000181 DAOMVE010000000
VERSION     DAOMVE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10341_length_2857_cov_1.747323"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="PLS64_08445"
     CDS             complement(<1..1727)
                     /locus_tag="PLS64_08445"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HRL43884.1"
                     /translation="MKRKEQEKLRWYLFLYRCARSISKSDEFLKEADSNQGDVINLLA
                     DKIIENARKNKFNIGNGNSDINAVKNFLKLIPKDVSDDTEAVKKIIFSASLNNIKLPE
                     VKEKTQLEAVVEYIAKTAKSNGYSFKARLWMPLLKEEIILSDITKSKFNGWNESDSWS
                     LNAVTGIYDDPENQAQLPLSVDFANGGHLAVCGSVVTGKSTFLQTLIYSLAVKYSPNV
                     VNFYILDFSSGMLSSFEKLPHTGGVLRENDVEKIGKFFSMIKGIIDERKKLFNGGNYR
                     QYVKVHGTEIPSIVIVIDNFAGFKEKTENAYEDTLIHLSREGVGYGIFLVLSSAGFGM
                     AEIQNRIGDNIRTVVSLEMGDKFKYMDVMRATHIDVIPESGIKGRGIAFVEGRLLEFQ
                     TALAIDAEDDYKRNAKIEAMSQKMRDMWDGNTAKQIPFIPENPVFDDLKNLDEYSVAV
                     SNKRLIPFAYNYVDASVYSIDLADTYCCSISGKRRTGKTNLLKLLMYAVSQKNGKGVV
                     IEKESNELKLLSSELGFEYIDSDKGVFDYFKSITDEFVARNKYKHTLEEDGLSDLQIY
                     EKMSAKEPM"
     assembly_gap    1743..1760
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1761..>2040)
                     /locus_tag="PLS64_08450"
     CDS             complement(<1761..>2040)
                     /locus_tag="PLS64_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43885.1"
                     /translation="TQKPSGTVDDNIWSNAKFRLCLRVQDRQDSNDMLHKPDAAYITQ
                     AGRCYLQVGNDEIYELFQSGWSGAPYDDSNEGGKQEIATMITPTGKTA"
     assembly_gap    2041..2050
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2051..>2461)
                     /locus_tag="PLS64_08455"
     CDS             complement(<2051..>2461)
                     /locus_tag="PLS64_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HRL43886.1"
                     /translation="KYHGPHGLIAGTTGSGKSELIQTFMLSLAINFSPDDVAFFVIDF
                     KGGGMANLFVDLPHMAGQISNLSGNQVRRAMISIKSENLRRQRIFGEYGVNNINNYTR
                     LYKSGEAPTPIPHLVIIIDEFAELKKEEPDFMREL"
     assembly_gap    2462..2471
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2472..2738)
                     /locus_tag="PLS64_08460"
     CDS             complement(<2472..2738)
                     /locus_tag="PLS64_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43887.1"
                     /translation="MSRYTEACSVHFDSVSSKKMNMFAKTLSGVAVREIKGDNSIAQS
                     LDFFEMYGVQKLDDFNIIEQWQKNRTFNTMKALVGKKAGGADCYL"
BASE COUNT          773 a          677 c          481 g          888 t
ORIGIN      
        1 aacatcggtt ctttagcgct cattttttcg tatatttgca gatctgacaa tccgtcctcc
       61 tcaagagtat gcttatattt atttcttgca acaaactcat cggtaattga cttaaagtaa
      121 tcgaaaactc ccttatcgct gtcaatatac tcaaagccga gttccgaaga aagcagtttt
      181 aattcattgc tttctttctc aatcaccaca cccttgccgt tcttttgact tactgcgtac
      241 ataagcaatt taagcagatt ggtttttccg gttctcctct ttccgcttat tgaacagcaa
      301 tatgtatccg ccaaatcaat gctgtaaaca gatgcgtcaa catagttgta tgcaaacggt
      361 atcaagcgtt tattgcttac ggcaacgctg tattcatcaa gatttttcaa atcgtcaaac
      421 acgggatttt ccggaatgaa cggtatctgt tttgccgtat ttccgtccca catatcacgc
      481 attttctgac tcattgcctc aatttttgcg tttctcttgt agtcatcctc tgcgtcaatc
      541 gcaagagcgg tctgaaattc aagcaatctg ccctcaacaa atgcaatgcc tctgccttta
      601 atacccgatt cgggaataac atcaatgtga gttgccctca taacatccat atacttaaac
      661 ttgtcgccca tttccagact tacaacggta cgaatattat cgcctattct gttttgaatt
      721 tcagccatac cgaatcccgc cgatgataac acaagaaaga taccgtagcc tacaccctct
      781 cgtgaaaggt ggatgagagt atcctcataa gcattctctg tcttttcctt aaagcctgca
      841 aagttgtcaa tcacaattac aatagagggg atttctgttc cgtgtacttt aacatactga
      901 cgatagttac cgccgttaaa cagtttcttt ctttcgtcaa taataccctt gatcatactg
      961 aaaaatttgc cgattttttc aacatcattt tcacgcaaaa caccgcccgt atgcggtaat
     1021 ttttcaaatg acgacaacat tccgctgctg aaatcgagaa tgtaaaaatt cacaacattc
     1081 ggagaatact ttactgcaag cgaataaatc aatgtctgta aaaatgtact cttacccgta
     1141 actacagaac cgcaaacggc aagatgaccg ccgtttgcaa aatcaaccga caacggcaat
     1201 tgtgcctgat tttccggatc gtcataaata cccgtcactg cattaagtga ccacgaatcg
     1261 ctttcattcc aaccgttgaa cttgcttttt gtaatatcgg aaagaatgat ttcttctttc
     1321 agcaacggca tccacagtct tgctttaaag ctatatccgt tgctttttgc ggtttttgca
     1381 atgtattcaa caacagcctc aagctgagtt ttttccttaa cctcgggtaa tttaatattg
     1441 ttaagcgacg ccgagaaaat aattttcttt accgcctcgg tatcgtcgga aacatctttc
     1501 ggaatcagct ttaaaaaatt ctttacggca ttaatatccg agttgccgtt tccgatatta
     1561 aacttattct tccttgcatt ttcaataatc ttatccgcaa gtaaattaat aacatcgccc
     1621 tgattgctgt ctgcctcttt taaaaattcg tcactttttg aaattgacct tgcacacctg
     1681 taaagaaaca gataccacct cagtttttcc tgctctttgc gtttcatttt tgtatgactt
     1741 ccnnnnnnnn nnnnnnnnnn atggcggttt tgcctgtagg agtaatcatt gttgcaatct
     1801 cctgcttgcc gccctcgttg ctgtcgtcat acggtgcacc gctccaaccg gattgaaaca
     1861 gctcatatat ttcatcattg ccgacctgaa gataacacct gccagcctgt gtaatataag
     1921 ccgcatccgg cttgtgcagc atatcgttac tgtcctgtcg gtcctgaaca cgcagacaaa
     1981 gtctgaattt tgcatttgac caaatgttat cgtcaaccgt accgctcggt ttctgcgttg
     2041 nnnnnnnnnn caactcacgc ataaaatcgg gttcttcctt tttcagctcc gcaaattcat
     2101 caattatgat aacaaggtgc ggaatcggtg tgggagcttc accgctctta tacaacctcg
     2161 tgtagttgtt tatgttattc acaccgtatt cgccgaaaat tctctgacgg cgaaggtttt
     2221 cgctcttaat tgaaatcatc gctctacgaa cctgattacc cgaaaggtta gaaatctgac
     2281 ctgccatatg cggtaaatca acaaacagat ttgccatgcc gccgcccttg aagtcgatta
     2341 caaaaaaggc aacatcatcg ggactgaaat tgattgcaag cgaaagcata aatgtctgaa
     2401 taagctcact tttacccgaa cccgttgtac ctgcaataag tccgtgaggt ccgtgatact
     2461 tnnnnnnnnn naagataaca atcagctccg cctgcctttt tgcccacaag agctttcatc
     2521 gtgttgaaag ttctgttttt ttgccattgc tcaataatgt taaaatcatc aagcttttgc
     2581 acaccgtaca tttcaaagaa atcaagtgat tgtgcaatgc tgttgtcgcc cttgatttct
     2641 cttacggcaa ctcccgacag agtctttgca aacatattca tttttttgct tgacaccgaa
     2701 tcaaaatgaa cggagcaagc ctctgtatag cggctcataa cattataaaa cccgcaaaat
     2761 tcactgtcgt tctgaattat attttcacac acattaggta aattacggca gtaatccgtt
     2821 ataagaaaag tcgtaagccc cgtgttattt tctctgt
//
LOCUS       DAOMVE010000182         2810 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10584_length_2810_cov_1.781125, whole genome shotgun sequence.
ACCESSION   DAOMVE010000182 DAOMVE010000000
VERSION     DAOMVE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10584_length_2810_cov_1.781125"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>646)
                     /locus_tag="PLS64_08465"
     CDS             complement(<1..>646)
                     /locus_tag="PLS64_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRL43888.1"
                     /translation="ELHIVDVEPTELQKELVDELSDRADEVNSGSVDPSQDNMLKITG
                     DGRKLGLDPRLISPDFEDDPNTKLNVCVNNVFDIYTDTAKDKLTQIVFCDLGVPSKNT
                     GNDSSKEQNDNKSVAELDSLEETGKFCVYDDIRDKLIAKGIPATEIAYIHNAKTEAQK
                     SELFSKVRSGEVRILLGSTGKMGTGTNVQDRIIALHDLDVPWRPADLEQRRGRMV"
     assembly_gap    647..656
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<657..1239)
                     /locus_tag="PLS64_08470"
     CDS             complement(<657..1239)
                     /locus_tag="PLS64_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43889.1"
                     /translation="MKAQDGNKFTVKEMERTKKNLENQLEKLQSSKKDNTVYFEELGI
                     DKLFVDEAHEFKNLYCATKLQNVSGISSRASQRATELFAKCRYLDEKTGGKGVVFATG
                     TPLSNSVTELHTMMRYLNYDFLASHNNMQNFDNWISAFGVQKKEYELAPAGNKFKERT
                     RIAEYSNLPELMSMFKMVADVKTAETLKLKVPDC"
     assembly_gap    1334..1343
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1344..>2810)
                     /locus_tag="PLS64_08475"
     CDS             complement(<1344..>2810)
                     /locus_tag="PLS64_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43890.1"
                     /translation="TLRELLDVQQYDNKDSEIAQLQEKLNNCYDSFYSKYGLLHSSYN
                     HSLLNSDGAYPLVASLEADFAKDNNLLVKKSDIFTERVNKPTATISHVDTAEEALAVC
                     VAEKGVIDFEYMSQITDVPPEDLKIGLREKGEIFAVPSYTGDEDYEYQTASEYLSGDI
                     YAKLDMAKAYASVNGIYESNVVALKEVLPTPLKAGDIDINLGATWIDKKYYEQFMYEV
                     FETPQSLKSDYVSRFFKSDRIELDYSEYSGRWNITNKRADLSVTTVKTYGASGLSAYQ
                     IFESVLNLSEPKVYKDKVDEYGVVERDKNNKPVRVLDVEATQVVQQKANAIRREFKNW
                     IFKDANRRRDIVDTYNRTFNCVHAREYDGSHLNFPGMNSNIQLHEHQKNAIAHAIYGG
                     NTLFAHSVGAGKTFEIIATAMECKRLGLCHKSLFAVPNHLTEQVGADFLKLYPNANIL
                     VATKDDFKSSNRKMLMSKIATGNYDIIVIGHSQFERIPL"
BASE COUNT          730 a          631 c          534 g          895 t
ORIGIN      
        1 tgaccattct gccacgcctc tgctcgaggt ctgccggtcg ccacggaaca tctaaatcgt
       61 gcagagcaat gattctgtcc tgcacatttg tgcctgttcc cattttgccc gttgaaccga
      121 gtaaaattct cacttcaccg cttctgacct ttgaaaacaa ttcagacttt tgagcttcgg
      181 tttttgcatt gtgtatatat gctatttcag tcgcagggat gccctttgca atgagcttgt
      241 cccttatgtc gtcatacacg caaaacttgc ctgtttcctc aagagagtca agttccgcaa
      301 cactcttgtt atcgttttgc tcttttgagg agtcattgcc tgtgtttttt gacggcacac
      361 cgaggtcaca aaaaacaatt tgagtaagct tgtcttttgc tgtgtctgta taaatgtcaa
      421 atacattgtt tacacacaca ttcagctttg tatttggatc atcctcaaaa tcgggactaa
      481 taagtcgggg gtcaagaccg agctttctgc cgtcaccggt aattttgagc atattatcct
      541 gtgacggatc tacggagcct gaattaacct catctgctct gtcggataat tcatcgacaa
      601 gctccttttg aagctctgta ggctctacat caacaatgtg aagctcnnnn nnnnnncaca
      661 atcaggcact ttaagtttaa gagtttcagc tgtctttaca tcggctacca tcttaaacat
      721 agacataagc tcaggcagat tggaatattc cgcaatcctt gtgcgttcct taaacttgtt
      781 tcctgccgga gctaactcgt attctttctt ttgcacaccg aatgcagaaa tccaattatc
      841 aaaattctgc atattgttgt gacttgcaag aaaatcataa ttaaggtatc tcatcattgt
      901 gtgaagctct gtaacgctgt ttgacaacgg tgtacctgtg gcaaatacaa cacccttgcc
      961 gcctgtcttt tcgtcaagat aacggcattt tgcaaacagc tctgttgccc tctgagaagc
     1021 tcttgaagat atacccgaaa cattttgcag ctttgttgca cagtacagat tcttaaattc
     1081 gtgagcttcg tcaacaaaca atttgtcaat tccaagctcc tcgaaatata cggtattgtc
     1141 tttcttactt gattgaagct tctcaagctg attttcgaga ttcttttttg tacgctccat
     1201 ttccttaacg gtaaatttat taccgtcctg agctttcatc tcagcaagac cggcaacaat
     1261 atcatcaatt tgagattgta aaaaggcttc ctgcctttca ggagataggg gcagcatctt
     1321 taattgcgta tgtnnnnnnn nnncagcggg atgcgctcaa actggctgtg cccgatcacg
     1381 atgatgtcat aattgccagt tgcaattttt gacattaaca tcttgcggtt tgatgattta
     1441 aaatcatcct ttgttgcaac caatatgttt gcgttgggat agagcttcaa aaagtctgct
     1501 cccacctgtt ctgtcagatg attcggtaca gcaaacaagg atttgtgaca caacccaagc
     1561 cgtttacatt ccatagcagt agctatgatt tcaaaggttt ttcctgcacc taccgaatga
     1621 gcaaacagtg tgttgcctcc gtaaatagcg tgagcaatgg cattcttttg atgttcgtga
     1681 agctgaatgt ttgaattcat tcccggaaaa ttaaggtgtg aaccgtcata ctctcgtgcg
     1741 tggacgcaat tgaatgtgcg attgtatgtg tcaacaatat ctctgcggcg gttagcatct
     1801 ttaaaaatcc aattcttaaa ttcacggcga atggcattgg ctttttgctg tacaacctga
     1861 gtcgcttcta catcaagcac tcttacaggc ttattgtttt tatcacgctc aacaacaccg
     1921 tattcgtcaa ccttgtcttt atacactttc ggttcggata aattgagaac ggattcaaat
     1981 atctgatatg cagataatcc gcttgcaccg taggtcttta cagtagttac cgacaaatcc
     2041 gctctcttat tcgtgatatt ccatctgccg gaatactctg aatagtcaag ctctattctg
     2101 tcggatttaa aaaatcttga cacataatcc gatttaaggc tttgcggtgt ttcaaaaacc
     2161 tcatacataa attgctcata gtatttttta tcaatccaag tagctccgag gttaatatca
     2221 atatcgccgg ctttcagagg agtaggcaat acttccttta aggcgacaac atttgattca
     2281 tatatgccgt tgaccgaagc gtatgctttt gccatgtcaa gctttgcata tatatctccc
     2341 gacaaatatt cggaagcagt ttgatattcg tagtcctcat cgcctgtata tgacggtacg
     2401 gcaaatattt cacctttttc tctcagcccg attttcaaat cctcgggcgg cacatccgta
     2461 atttggctca tatactcgaa gtcgataaca cctttttctg caacgcagac agcaagagct
     2521 tcttcagccg tatcaacatg acttattgtt gctgtcggct tatttacacg ctctgtaaat
     2581 atatcggact tcttaacaag taaattatta tctttggcaa aatcggcttc gagagaagca
     2641 acaagcggat atgctccgtc agagtttaac aagctgtgat tgtaactgct gtgaagcaag
     2701 ccgtacttac tgtagaagct gtcataacag ttgttgagct tttcctgcaa ttgagctatc
     2761 tctgaatcct tgttatcgta ctgctgaaca tcaagcaatt cacgcaaagt
//
LOCUS       DAOMVE010000183         2783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10728_length_2783_cov_2.217742, whole genome shotgun sequence.
ACCESSION   DAOMVE010000183 DAOMVE010000000
VERSION     DAOMVE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10728_length_2783_cov_2.217742"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..520)
                     /locus_tag="PLS64_08480"
     CDS             complement(56..520)
                     /locus_tag="PLS64_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43891.1"
                     /translation="MLNSATLTAILVNILAALFFTCITICIATHFAKKKANEADKKLI
                     NNKCYGIIAVIFTIINFIASFTAIAVAYVADSTDYYHISIPKFSYLSLILVAGIIEFL
                     VVKAIVFKKISCSKKERAKQAAIFAAICTPYYIIAANLPIIMYMVAVALDPC"
     assembly_gap    581..590
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(620..1105)
                     /locus_tag="PLS64_08485"
     CDS             complement(620..1105)
                     /locus_tag="PLS64_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43892.1"
                     /translation="MNENSNPEILITIIILSVLLAILSVTTAFCHTERSAGYKNKVNI
                     GKKNHIIIYPIFFVLHMTAILLFNLPFPDLTCYLSFSIGSIVFIFLIQAVIAYFTMFK
                     KFECDTGTKVGKSIVFSLISTPFYSILFGVAFAFFFPYSVGIPILMIIGLFIYCITSY
                     R"
     gene            complement(1124..1588)
                     /locus_tag="PLS64_08490"
     CDS             complement(1124..1588)
                     /locus_tag="PLS64_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43893.1"
                     /translation="MLNSATLAVILLYILSAILFTSTTVSIAIYYARKKADTEEKTLI
                     TKNDYKKIILIFSIINFIASFAATAVAHIADSGDYNHLSIGQFSYLSLIFVAGVVEFF
                     IAKSTIFQRFSCSKADKTERAAVFAAICAPYYIFIVSSPIVPYIIAVSLYPC"
     gene            complement(1614..2156)
                     /locus_tag="PLS64_08495"
     CDS             complement(1614..2156)
                     /locus_tag="PLS64_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43894.1"
                     /translation="MKYTYIYVILGICAVAVSIGKALAVNFGISKYLSKKSYNGKFKV
                     IKTASISFGVGYILLALASLFIVTMVMDAIYSHIRFDQLLQDFLSIFYMAIIGANYEF
                     LDSPYGLGLIYVFPFIFVIIVSIVVLIFVNYTFVYRKFGIPNNKKWKLSFFTALANAP
                     YELLIPYGQIASMIINRMMF"
     gene            complement(2175..>2783)
                     /locus_tag="PLS64_08500"
     CDS             complement(2175..>2783)
                     /locus_tag="PLS64_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43895.1"
                     /translation="RDIVLYNCTNELVQLLKKERDFMLKFILYMLLAVVILSGGIFKT
                     FAINYGISKYLSKKPYKGDFEFKKVALHTLKTNYILLALVVLIVLGITLCSASSYINI
                     VENLVELILGFFIALTMSLLGPWYNTIGNEYGAYVIPFFILVIISIPMLFLINYKFVY
                     KDFEVSKNKKMKLSFFTALANAPYELLIPYGQFGTMIFKSMI"
BASE COUNT          995 a          443 c          462 g          873 t
ORIGIN      
        1 cggtgggcat ctgctcaccg ttttttatgt ttttcataat atcacttcgt taaaatcagc
       61 aggggtcgag agctactgcg accatataca taattatcgg taagttggcg gcaataatat
      121 agtatggagt acatattgcc gcaaaaattg cagcctgttt ggctctttct ttttttgaac
      181 aggaaatttt cttgaatact attgctttga caacaagaaa ttcaattatg cctgccacca
      241 atattaaaga taaataagaa aattttggga ttgagatgtg gtagtaatca gtcgagtctg
      301 caacatatgc aacagctatt gcggtgaatg acgcaataaa gtttataatt gtaaaaataa
      361 cagctattat tccgtaacat ttgttgttta taagtttttt atctgcttca ttagcttttt
      421 tcttggcaaa atgtgtagca atgcagattg tgatacaagt aaaaaataaa gccgcaagaa
      481 tgtttactaa aatagcggta agagttgccg agttaagcat aaatcatctc tccttaaaag
      541 aaaatcatct ttatgaaaag tgcatatgga ataaacaggt nnnnnnnnnn gctcaccgtt
      601 ttttatgttt ttcataatat cacctataac ttgtaatgca gtaaatgaat aagccgatta
      661 tcataagaat cggaataccg acactgtacg ggaaaaagaa tgcaaaagcc acaccaaaaa
      721 ggatactgta gaatggtgtt gaaatcagcg aaaaaacaat tgatttgccg acttttgtac
      781 ccgtatcgca ttcaaacttt ttaaacattg taaaataagc aataacagct tgaatcaaaa
      841 atataaaaac aatacttcct atgctgaatg aaagatagca agttaagtca ggaaacggaa
      901 ggttaaaaag cagtattgct gtcatatgca aaacaaaaaa tatcgggtaa attattatgt
      961 gattcttttt gccgatgttt actttgtttt tgtaacccgc acttctttcc gtatggcaaa
     1021 atgctgttgt aacagaaagt atggcaagaa gtacagataa aataattata gtgattaaga
     1081 tttcgggatt tgagttttca ttcataatat cacttcgtta aaatcagcag gggtaaagag
     1141 atactgcgat tatatacgga actatcggtg agcttacgat aaatatgtaa tacggtgcgc
     1201 atattgcggc aaagactgcg gctctttccg ttttgtctgc ctttgaacag gaaaatcttt
     1261 ggaatatagt tgactttgca ataaaaaatt caactacgcc tgccacaaat attaaagata
     1321 aataagaaaa ttgtcctatt gaaaggtggt tgtaatcacc tgagtctgca atatgtgcaa
     1381 cagccgttgc ggcaaatgat gcaatgaagt ttataattga aaaaatcaga attatttttt
     1441 tgtaatcatt ttttgttata agtgtttttt cttctgtatc agcttttttt cttgcataat
     1501 atatagcaat cgaaacggta gtagatgtaa aaagtatggc tgaaagtata tataataata
     1561 taacagcgag agttgccgag ttaagcataa atcatctctc cttaaacaca aaatcaaaac
     1621 atcatacggt tgataatcat acttgcaatc tgtccgtaag ggatcaacaa ttcatacggt
     1681 gcgtttgcaa gtgcggtgaa aaatgagagt ttccattttt tattgtttgg tattccaaat
     1741 ttgcgataaa caaatgtgta gttcacaaaa atcaatacca ctatgcttac gataataacg
     1801 aaaataaacg gaaaaacata tatcaaacca agaccgtatg gactatccaa aaattcataa
     1861 tttgctccga taatagccat gtaaaatata gataagaagt cctgcaataa ctggtcaaat
     1921 cttatgtgtg agtaaattgc atccatcacc atcgtcacaa taaataaaga agctaaagcc
     1981 aataatatgt atccgactcc gaatgaaata gaagctgttt ttatgacctt gaatttaccg
     2041 ttgtaagatt ttttagaaag gtattttgaa ataccgaaat ttactgctaa agcttttcct
     2101 atgcttactg caacggcaca aatgccgagt ataacatata tgtatgtgta tttcataacc
     2161 aagcctcctt aacattaaat catacttttg aaaatcatcg ttccaaactg accgtatggt
     2221 attaacaatt catacggtgc gtttgcaagt gctgtgaaaa atgaaagttt cattttctta
     2281 tttttagaaa cttcaaaatc tttgtaaacg aatttgtaat tgataagaaa cagcattgga
     2341 atgcttatga taactaaaat aaagaaaggt ataacatacg caccgtactc attaccgatt
     2401 gtattgtacc atggacctaa aagagacatt gtaagtgcaa taaaaaatcc taaaatcaat
     2461 tctactaaat tttctactat attaatgtaa ctcgatgcac tgcaaagcgt tatacctaaa
     2521 acgatcagaa caactaatgc caacaaaata tagttcgttt tcaaagtatg taacgcaact
     2581 tttttgaatt caaaatcgcc cttatatggc ttttttgaga ggtattttga aataccatag
     2641 tttatcgcaa aggttttaaa gatacctccg ctgagaatta caacagcaag caacatatat
     2701 aagataaatt tcaacataaa atcacgctcc tttttaagca attgaactaa ttcattagta
     2761 caattatata atacaatatc tct
//
LOCUS       DAOMVE010000184         2777 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_10753_length_2777_cov_2.690669, whole genome shotgun sequence.
ACCESSION   DAOMVE010000184 DAOMVE010000000
VERSION     DAOMVE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10753_length_2777_cov_2.690669"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLS64_08505"
     CDS             complement(<1..891)
                     /locus_tag="PLS64_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRL43896.1"
                     /translation="MLRILHIVTYMGRGGLETMIMNYYRNIDRTKIQFDFLVHRQEKA
                     DYDDEILSLGGHIYHMPMLNPFSKAYFNALDDFFDNHKYDIVHSHLDCMSAYPLKIAQ
                     KHGVKVRIAHSHNKSQDKNLKYPIKLLSKHLIPKYATHLFSCGKEAGDWMFGGNAYTV
                     LNNAIDAEKYRYDCQIRNQVRDELKISKSDFVIGHVGRFNPPKNHPFLIDVFKSVHDK
                     NRNSKLLLVGTGNGQSNIREKVDNLGLSDSVFFLGNRGDVNRILQAMDVFLFPSLYEG
                     LGIAALEAQASGLPCIISDGV"
     gene            complement(921..2012)
                     /locus_tag="PLS64_08510"
     CDS             complement(921..2012)
                     /locus_tag="PLS64_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRL43897.1"
                     /translation="MKNNRKKVMILTNHSYMLYRFRKELIEELMKNYDVVLSMPFVGH
                     HEDFINMGLRCIETKMERRGVNPIHDFSLMKFYKKILQEENSDLVITYSIKPNVYGGL
                     MCEKLNIPFYANVQGLGTAFQKPGLAQFATVLYKTAFKKVKTVFFENQVNADEFVDRK
                     IISVEKETILNGAGINLEIYEYKPYPKSDPPHFLYLGRIMKEKGMDELFSAAEKLHDD
                     GYKFVLDLVGFFEDEYKERVELLESKGVVKFHGFQEEPRPYYATADCVVMPSYHEGMS
                     NVNLEASATGRPVITTDIPGCRESVENEYTGWLCEPKSVDSLYEKMKAFLETDISKRE
                     VMGKYARQKMVDEFDKEIIVNDTIKALKL"
     gene            complement(2002..2615)
                     /locus_tag="PLS64_08515"
                     /pseudo
     CDS             complement(2002..2615)
                     /locus_tag="PLS64_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
BASE COUNT          893 a          586 c          396 g          902 t
ORIGIN      
        1 aacaccatca gaaataatgc acggcaatcc tgaagcctgt gcttcaagtg ctgcaatacc
       61 taatccttcg tataaagacg gaaacagaaa tacatccatt gcctgtagta ttctattcac
      121 atctcccctg tttcctaaaa aaaacacgga atccgataaa ccaagattat caactttttc
      181 tcttatatta ctctgtccgt tacctgttcc tacaagaagc agtttggaat ttctgttttt
      241 atcatgtaca ctcttaaaaa catcaatcaa aaacggatga tttttgggtg gattaaatct
      301 tccgacatgt ccgattacaa aatcagattt actgattttc agttcgtcac gcacttgatt
      361 tcttatttga cagtcatacc gatatttttc agcgtcaatt gcattattta agacagtata
      421 tgcatttccg ccaaacatcc aatctcctgc ctctttaccg caagaaaaaa gatgcgtagc
      481 gtacttagga attaaatgtt ttgaaagaag cttaatcgga tactttaaat ttttgtcttg
      541 acttttatta tgcgaatgtg caattctcac cttaacgcca tgtttttgtg caattttcaa
      601 aggatatgca ctcatgcaat ccaaatgtga atgcacaata tcgtacttat gattatcaaa
      661 gaagtcatca agcgcattaa aataagcctt agaaaaggga ttcagcatag gcatatgata
      721 aatatgtccg cctaaagata atatctcatc atcataatca gccttttcct gacgatgcac
      781 aagaaaatca aattgaattt tagtacggtc tatgtttcta taataattca taatcatagt
      841 ttcaagaccg ccacgcccca tgtatgttac aatatgtaat attcttaaca taaaattcaa
      901 ttacccttca ttttatagaa ttaaagtttt aaagccttta ttgtatcgtt tacaataatt
      961 tctttatcaa attcgtcaac cattttttgt cgggcgtatt tacccataac ttctcgtttt
     1021 gaaatatctg tttcaagaaa agctttcatt ttttcataaa ggctgtcaac gcttttaggt
     1081 tcacaaagcc agcctgtata ttcattttcc accgactctc tgcagcccgg aatatcggtg
     1141 gtaatgacag gtctgcctgt tgccgaagct tcgagattta cattactcat accctcgtga
     1201 tagctcggca ttacaacaca atcggcggtc gcatagtaag gtcttggttc ttcctgaaat
     1261 ccgtggaatt ttacaacgcc cttactttca agtaactcaa ctctttcctt gtattcatct
     1321 tcaaaaaagc ctacaaggtc aagtacaaat ttatagccgt catcgtgaag cttctcagcc
     1381 gcagaaaaca attcatccat gcccttttcc ttcatgattc tgccaagata caaaaaatgc
     1441 ggcggatcac ttttcgggta aggcttgtac tcataaattt caagattgat tcctgcgccg
     1501 ttcaaaatag tttctttctc gacagaaata atctttctgt caacaaattc atctgcatta
     1561 acctgatttt caaaaaatac cgtttttacc tttttaaagg ctgttttata aagaactgtc
     1621 gcaaactgag caagtccggg cttttgaaat gccgttccta atccctgaac attcgcataa
     1681 aacggaatat ttaacttttc acacatcaat ccaccgtaaa catttggttt gatagagtat
     1741 gtaattacca aatcagagtt ttcttcctgt aatatttttt tataaaattt cataagtgaa
     1801 aaatcgtgaa tcggattaac ccctctgcgc tccatcttag tttcaataca acgcaatccc
     1861 atattgatga aatcctcatg atgtcctaca aaaggcatac taagaacaac atcataattt
     1921 ttcataagtt cttcaatcaa ttcttttctg aaacgataca gcatataaga atgattggta
     1981 agaatcataa cttttttacg attattcttc atctgcaatt gctcctgttc cgccctcaac
     2041 tacaccatca tgcttaaaca cacttacaac tgtcataaaa agacatttta catcaaagac
     2101 aaaactgaca ttatcacaat aaatcttatc catctgaact ttttcaggga taggaagctc
     2161 atctctgccg ttaatctgcg cccaacccgt caagccgggt tttaaatcat ttataccgta
     2221 ctcatctctt tcagaaatca aatcatcctg attccaaagt gcaggtcttg gaccgataac
     2281 cgacatatcg cccttaaaaa tattaaaaat ctgaggcaat tcgtcaagac ttgtctttct
     2341 taaaaacgca cccaccttag ttatatactg ttcaggattt ttaagaagat gtgtaggaca
     2401 atccttggga gtatcaattc tcattgtgcg gaatttcaga atattgaaat attttttgtt
     2461 ttttcctatt cttttctgcc taaaaaaaca ggaccttcgc tcgtgcattt tattatggca
     2521 catatcggaa ggtaaagtac tgacagacac actatcgctg caagtgaaca gataatatca
     2581 agcattcttt tcacaattct gttataaaac gacatttttc tgtctttcat atttaatact
     2641 cctcaaccca aaactaactt acgcaataca tctatagaat tataccacag aaagcatcaa
     2701 taagcaataa aagaggagaa tatggaaaaa acaattaaaa aaaagtgctt tttaacaaaa
     2761 tatagggata attacgc
//
LOCUS       DAOMVE010000185         2733 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_11004_length_2733_cov_1.835325, whole genome shotgun sequence.
ACCESSION   DAOMVE010000185 DAOMVE010000000
VERSION     DAOMVE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11004_length_2733_cov_1.835325"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    513..522
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            544..>1353
                     /locus_tag="PLS64_08520"
     CDS             544..>1353
                     /locus_tag="PLS64_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRL43898.1"
                     /translation="MKNFNDDYYAGFDIGTDSVGYAVADTDYNLCKFKGNAMWGVDLF
                     EESNSAAERRTLRSARRRGLRKRNRIEWLQMLFDEEISKVDNAFYQRLKESCLYLDDK
                     SSNVPYAVFADGNYTDKEFHTDYPTIYHLRKELIKSSQPHDIRLVYLALHHIIKHRGH
                     FLFDNMGSDFESESSFEALFDDLKLYLKEEYEIEFECNDSLRFSEILKDKTLKKTAKS
                     SESYKLFGYSKRNNPYETALIDLMCGRKVKFSDMFGDKSFDSEEVNGITFES"
     assembly_gap    1354..1363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1364..>2733
                     /gene="cas9"
                     /locus_tag="PLS64_08525"
     CDS             <1364..>2733
                     /gene="cas9"
                     /locus_tag="PLS64_08525"
                     /inference="COORDINATES: protein motif:HMM:NF027906.2"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRL43899.1"
                     /translation="DDNENTYRDLLQEKFELIEKAKAVYDWAILADILNGEKYNGKKY
                     ISFAKVKIYEEHSSDLKMLKDFVKERCKSLYGEIFRITKDKLDNYTAYCGKYKENGRN
                     GVIQYRTNQADFCKYLKKKFEKLDKTGYEEMFDKIENGTFMPKIVVKDNGVIPMQVNR
                     SELKAILKNASTYLEFLNKKDENGISVSDKIVKIFEFRIPYYVGPLNNHSLKSWLVRS
                     DEKIYPWNFDSVVDIEQSAENFINNLTSKCTYLPTKDVIPKNSILYSAFTVLNELNNL
                     RLDGKKPDVSLKQAIFNDLFMTHKKVRRKDLLNYLKSEKGITPDITGIDGDFKSSMRS
                     AIEMSQFNLTDSEKEDAIKAITVFGDDKKLLRKRLKRQLGSKLSDEDIMRISKLKYKD
                     WGRLSKEFLTEVYNVDKNTGELQFNIIHALWQTNDNLMELLGSKYGFEQSRQNYLDGI
                     QTGQSL"
BASE COUNT          999 a          378 c          535 g          801 t
ORIGIN      
        1 cgatgtcttt tgcagagaac attcgagcag aaccctaaga gcttttctgt aaaatatctg
       61 tagagcatat tttgtcctcc gtttcaggtt tttaagcaat tccttttaac ggattttggc
      121 aaaatatgct ctactttttt tgctttttat ttgcagctct ttttctgccc taacttttat
      181 tatagagcaa tatttgaaat agaagggcgt tgatgagggt taaggttacg ctccgcaagt
      241 aattggcggg gcagaaatct tttgatttta aaaatgtata atacaacata ctgatagtag
      301 tatgttatac tacgcaaaat atatagaaag tataccaaaa ttgtacaatt gaataaaata
      361 tactaaaaat aaggataaaa taacttaaag atattgaaaa taactacagc gtgtgctaaa
      421 attagacaaa agaaaaggat tcggaaaacc gaatcctcaa atcacacact gtgatggttg
      481 cctaagcaac ggatagaatt aatttttatt tgnnnnnnnn nncaatagaa aaggaggaaa
      541 tttatgaaga attttaatga cgattattat gcaggatttg atataggaac ggattctgtc
      601 ggatatgcag tagccgatac agattacaat ctttgcaaat ttaaaggcaa tgctatgtgg
      661 ggtgttgatc tgtttgagga gagtaattca gctgccgaga gaagaactct tagatcggca
      721 cgaagaagag gtttgagaaa aagaaaccgt attgaatggc tgcaaatgct ttttgatgag
      781 gaaatttcaa aagttgacaa tgctttttat cagagattaa aagaaagttg tctgtatctt
      841 gatgacaaat catcaaatgt tccgtatgcg gtttttgcag acggtaatta caccgataaa
      901 gaattccata cagactaccc tacaatatat catcttagaa aagaattgat taagagctca
      961 caaccacacg atataagact tgtatatctt gcgttacatc atattataaa acacagggga
     1021 catttcttat ttgataatat ggggtctgat tttgaaagtg aatcctcgtt tgaagccctt
     1081 tttgacgatt taaaacttta cttaaaagaa gaatatgaaa ttgaatttga gtgcaatgat
     1141 tcgttaagat tttcagaaat attaaaggac aaaacattga agaaaactgc aaaatcgtcc
     1201 gaatcgtaca agcttttcgg ttacagtaaa agaaataatc catatgaaac cgctttgata
     1261 gatttgatgt gtggcagaaa agttaaattt tccgatatgt tcggtgataa gtcgtttgac
     1321 agtgaggaag taaacggtat tacctttgaa agcnnnnnnn nnnatgacga taatgaaaat
     1381 acataccgtg atttgttaca agaaaagttt gaacttatcg aaaaagcaaa ggcggtttat
     1441 gactgggcaa ttcttgcaga tattttgaac ggtgaaaagt ataatggtaa aaagtatatt
     1501 tcatttgcca aagttaaaat atacgaagaa catagttcag atttgaaaat gctcaaagat
     1561 ttcgttaaag aaagatgtaa atcgttatat ggcgaaatct ttagaattac aaaggacaag
     1621 cttgataact atacggcata ttgcggaaag tacaaagaaa acggcagaaa cggtgtgatt
     1681 cagtatcgta caaatcaggc agatttctgc aaatatctga agaaaaagtt tgaaaagctt
     1741 gacaaaacag gctatgaaga aatgtttgat aagattgaaa acggtacatt tatgccgaaa
     1801 attgtcgtaa aagataacgg cgttattcct atgcaggtga ataggagtga gctgaaagca
     1861 attctgaaaa atgcttcaac ttatcttgaa tttcttaata aaaaagatga aaacggaatt
     1921 tctgtaagtg acaagatagt caaaattttt gaattcagaa taccgtatta tgtaggtccg
     1981 ctgaataatc acagcctaaa atcctggctt gtcagaagtg atgaaaaaat atatccgtgg
     2041 aattttgaca gtgttgttga catagagcaa tcggcagaga attttataaa taatctgact
     2101 tcaaaatgta catatcttcc aacgaaggat gtaataccta agaattcgat tttatacagt
     2161 gcttttacgg ttcttaatga actgaataat ttaagacttg acggaaaaaa gcctgatgta
     2221 agtcttaaac aggcaatatt taatgacttg tttatgacgc ataaaaaggt cagaagaaaa
     2281 gatttgctaa attatctaaa aagcgaaaaa ggtattacgc ccgatatcac aggcattgac
     2341 ggtgatttta agtcaagtat gcgttccgca attgaaatgt cacagtttaa tctgaccgac
     2401 agcgaaaaag aggacgctat aaaagctata actgtatttg gcgatgataa aaaactttta
     2461 agaaagcgtt taaaaaggca gctcggttca aaattatcgg atgaagatat aatgagaatt
     2521 tctaaactca aatacaagga ttggggaaga ttatcaaagg agtttcttac cgaagtttat
     2581 aatgtcgata aaaatacagg cgagttgcaa tttaacatta tccatgcact atggcagaca
     2641 aatgataatc ttatggagct gcttggctca aaatacggtt ttgaacagag cagacaaaac
     2701 tatcttgacg gtattcagac agggcaaagc ctt
//
LOCUS       DAOMVE010000186         2717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_11097_length_2717_cov_2.200977, whole genome shotgun sequence.
ACCESSION   DAOMVE010000186 DAOMVE010000000
VERSION     DAOMVE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11097_length_2717_cov_2.200977"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..918
                     /locus_tag="PLS64_08530"
     CDS             223..918
                     /locus_tag="PLS64_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRL43900.1"
                     /translation="MNIAIVDDEPVFLKQLHNRLREMKLPDCEIHEFTSGRDLLSFYV
                     KGMYEVIILDVEMPDLTGLQTAERIRQIDGSVIISFLTNYAEFAVKGYDVGAFRYILK
                     NQPDYVYTKQLNSIIAECQQRFRTFTFNNKNLSFKFRLTDILYFEGHRRKVSLFTVNG
                     ELEYGGDFSTVCGELLKYNFAIINRGILVNLDHIQNITKYDIILSNGRKIPIGKTYKD
                     EIVARYLNYATGR"
     gene            924..2192
                     /locus_tag="PLS64_08535"
     CDS             924..2192
                     /locus_tag="PLS64_08535"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRL43901.1"
                     /translation="MPVLIENIIEIVNVAIEMMLVFLYFSLLSKAKVNKAVLYLSYLL
                     STVILSATVLLTDNILVYLITTIILISSMSFICYDDSIRHKIFWNILFLLIISISEPI
                     VIGLLCIANMGTPDEFLESGLGRYLGMVGTNIIYLWLIGLMHRIINKKIRELPIKYWI
                     LIIVIPIISIFLLQTMLDGFVGSNTYSYVSLGVSLAGIIFINLAMFNFFESFEDKIKL
                     KYLETIKQQEQENYKLLTLSYKQVREFKHDIENQFLVLNDMLENNDTDSAKEYLVKLS
                     SFVRLGNNAVDSIVNIKGSLAQTYGIEFICKVNIITNIKADELELCRIIGNALDNAIE
                     GCQRAGVSHKHIWISLMEEREKLFIVITNTSDKVDTSVLTSTKKEQGLHGIGINSIKS
                     SVDRLGGLVSFDYDDGIFKLNIMVRNCLQI"
     gene            2311..>2717
                     /locus_tag="PLS64_08540"
     CDS             2311..>2717
                     /locus_tag="PLS64_08540"
                     /inference="COORDINATES: protein motif:HMM:NF016526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HRL43902.1"
                     /translation="MISKLAKSIAHFFVVQNITEESKEVIYAYGMELLISDVLNTIIV
                     LLIALFSHTLPAVVVFIAVFMGLRQFVGGFHANSHLSCMFTLVMVMMVFSYGICNVSG
                     HITPIFSISFIMIALPIILCIAPVPHPNKPMSE"
BASE COUNT          854 a          446 c          534 g          883 t
ORIGIN      
        1 atttcggcgt tctctcgctg tcttctgtga agcacttata agtacaccca ctcttaaatc
       61 aggcaataga ccatattcga tgaatataaa ataacgcaac atgaggctat gtctccgaaa
      121 ctattaatgg aggcatagct attttttctc ttgaaataat ctctccgatg tggtataatg
      181 ataactgata cataaagcat gaccaattgt gaggtgattg ctatgaatat tgctatcgtt
      241 gatgatgaac ctgtattctt aaaacaactt cataacagat taagagaaat gaaactcccc
      301 gactgcgaaa tacacgaatt tacaagcggg agagacttat tgagtttcta tgtcaaggga
      361 atgtatgaag tcatcatact cgatgttgaa atgcccgatc tgaccggatt gcagaccgct
      421 gaaagaatca gacagataga tggttcagtt attatttctt tcctgacgaa ttatgccgaa
      481 tttgccgtta agggctacga tgtgggagcg tttcgctata tattgaaaaa tcagccagac
      541 tatgtatata ccaaacagct aaattctatc attgctgagt gccagcagcg atttagaact
      601 ttcactttca ataataaaaa tctgtcattc aagttcaggc tgacagatat tctttacttt
      661 gagggacata gaagaaaggt atcactattc acagttaatg gtgaattgga gtacggcggc
      721 gatttttcaa ctgtgtgtgg cgagttgtta aagtacaatt tcgctattat aaaccgtgga
      781 atacttgtaa atcttgacca tatacagaat attaccaagt acgatattat tctaagcaac
      841 ggcagaaaaa tccccatagg caaaacatat aaagatgaga tagttgctcg ctatcttaac
      901 tatgcaacag ggagatgatc gagttgcccg tattgattga aaatatcatt gagattgtca
      961 atgtagcaat tgaaatgatg ttggtattct tatacttttc cttgttgagt aaggcaaaag
     1021 ttaataaagc agttctttat ctgtcgtatc ttctttctac tgtaatactg tcagcgacag
     1081 ttttgttgac agataatatt ttagtgtatc tgattacaac cattattctt attagctcca
     1141 tgtcctttat ttgttatgat gattcaatcc gtcataaaat attttggaac atattgtttt
     1201 tactgattat atctatatct gaacctattg tgattggatt actttgcatt gctaatatgg
     1261 gaacaccaga tgaattctta gagtctggtt taggacgtta tctcggaatg gtaggcacaa
     1321 atataattta tttatggctt ataggattga tgcaccgcat aataaacaaa aaaattcgtg
     1381 aactgccgat aaaatattgg atattgataa tagttattcc aatcataagt atttttcttt
     1441 tacaaacaat gcttgatgga tttgttggca gcaacactta tagttatgtt tctttgggcg
     1501 tttctcttgc aggaattatc tttataaacc ttgctatgtt taatttcttt gaaagttttg
     1561 aagataagat aaagttaaaa taccttgaaa caataaagca acaggaacag gaaaactata
     1621 agctgcttac cctgtcatac aagcaagtac gagagtttaa acacgatatt gaaaatcaat
     1681 ttttagttct gaatgatatg ttggaaaaca atgatactga ttctgctaag gaatatcttg
     1741 tcaagctaag ctcatttgta aggcttggta ataatgccgt cgattctatt gtgaacatca
     1801 agggttctct tgctcagaca tacggtatag agtttatttg caaggtcaat atcataacaa
     1861 atatcaaggc agacgaattg gagttgtgcc gtatcatcgg aaacgctctt gacaatgcaa
     1921 tagaaggttg ccagagagct ggtgtatcac ataagcatat ttggataagc ctaatggaag
     1981 aacgagagaa gctatttatt gtcattacaa atacatcaga taaggtagat acatctgttt
     2041 taacatcaac aaagaaagaa caagggcttc acggcatagg gataaacagt atcaaatcct
     2101 ccgttgatag attgggtggg ttagtaagtt tcgattatga tgacggaatt tttaagctga
     2161 acattatggt tcgtaattgt ttacaaattt aactatttgc atttcgggac aagaagacga
     2221 catttgggga cactttgaga ccttttttct caaaacctat tgaaaaaatt tgcaacttgt
     2281 gttatgattt ttgcaggaag tgattcagaa atgatttcaa aacttgcaaa aagcatagca
     2341 cacttttttg tggttcagaa cataacggaa gaatccaaag aggtgatcta cgcatatgga
     2401 atggagctgc tgatctctga tgtgctgaat accattatcg tcctgctgat tgctctgttt
     2461 tcccatacgc tgcctgcggt tgttgtattc atagccgtat ttatggggtt gagacagttt
     2521 gtaggtgggt ttcacgctaa cagtcatctt agttgtatgt tcacgcttgt catggtgatg
     2581 atggttttct cttacggcat ttgcaatgta agcggacata ttacgccaat attcagcatc
     2641 agtttcataa tgatagcatt accaatcatt ctatgtattg ctcccgttcc gcatccgaat
     2701 aaaccgatgt cagagga
//
LOCUS       DAOMVE010000187         2704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_11178_length_2704_cov_1.655342, whole genome shotgun sequence.
ACCESSION   DAOMVE010000187 DAOMVE010000000
VERSION     DAOMVE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11178_length_2704_cov_1.655342"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLS64_08545"
     CDS             complement(<1..240)
                     /locus_tag="PLS64_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43903.1"
                     /translation="MTAKSITISVKGKRTIGDAGNDQIAALAFENGRKTEVSFMWTFP
                     NGATVLFKNAVVSVTSNGAGAGTGVAPLEFEVMSNG"
     gene            complement(188..355)
                     /locus_tag="PLS64_08550"
     CDS             complement(188..355)
                     /locus_tag="PLS64_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43904.1"
                     /translation="MAKVESGVFPCYENQFAVGKAGTESATTNIANCEEFKAYDSKVN
                     HNFGKGQAYNR"
     gene            complement(360..752)
                     /locus_tag="PLS64_08555"
     CDS             complement(360..752)
                     /locus_tag="PLS64_08555"
                     /inference="COORDINATES: protein motif:HMM:NF024103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HRL43905.1"
                     /translation="MIYLSDIRDWLKSVTSAEHYYIGKLDNKQDRSIGVYSLKQSGTP
                     TRAIGGESTYDTISVSLLIHYTDNARETEEFARRLYETLYGIKNVEIKEHKIYIIELL
                     TEEPVDVGTDDKGVYEQVIEVKFYYERK"
     gene            complement(749..1135)
                     /locus_tag="PLS64_08560"
     CDS             complement(749..1135)
                     /locus_tag="PLS64_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43906.1"
                     /translation="MGFSVSSKIKLNMPVVKQLDRAKQQALEQTGDALLKQVKNTQVM
                     PFDTGNLQNENTFEDCVQSWNGTVKIVSSTPYARRLYFHPEYNFSRKENIAAGGKWFS
                     PWLEGGTRQNFCSRAFVRLYRKEAGL"
     gene            complement(1135..1527)
                     /locus_tag="PLS64_08565"
     CDS             complement(1135..1527)
                     /locus_tag="PLS64_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43907.1"
                     /translation="MKFPSLVKKQFCKTPVEVTIYGEGVTEDGAPLTVFECKNLYPSD
                     SLYPSATLHGGSALCNMQSKAKTVYTKEQKIVQVSAVLLFDGDIAPDSPTLSGGFVIL
                     DGVKRNIVQGTKHRNPDGKVNFTELDVI"
     gene            complement(1544..1873)
                     /locus_tag="PLS64_08570"
     CDS             complement(1544..1873)
                     /locus_tag="PLS64_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43908.1"
                     /translation="MYADYIEHQGGDENSIISAEHIDVLTFNRIDFEKLSEMQKRIIG
                     RVHSRLTAFEEENADMISSYLKSYSINGTSMEFGASWNLMCISGVAIPADLYALLKST
                     GLCYPAI"
     gene            complement(1873..>2704)
                     /locus_tag="PLS64_08575"
     CDS             complement(1873..>2704)
                     /locus_tag="PLS64_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HRL43909.1"
                     /translation="GARTFHFTQMSTTGFKNHSREGGWNKGKYTQTDVPFTCEHDRDI
                     EFLVDKADVDETNATAKVENISKVFEQTQVAPETDALFFSKVAAKAQATDGYHSSTKT
                     SEWTKENAYSKLKTILSAGKLRRYKARGTLVAYVTSHIMDCLEQSTEFTRKIELTQIA
                     EGGIGIETRVTEIDGCPIIEVIDDERFYDNFNFNPDDGGFEPATGAHKINVLVACGET
                     CKTVPKISSIYFFAPGSHTEGDGWLYQNRSLSDTFVFPNGKDGKIDSIYADVDTTAVA
                     "
BASE COUNT          660 a          646 c          585 g          813 t
ORIGIN      
        1 gccgtttgac ataacttcaa attcaagcgg agcaacaccc gtacctgcgc ctgcaccgtt
       61 tgatgtaacg gatacaactg catttttaaa gaggacggtt gcaccgttgg ggaaggtcca
      121 cataaacgaa acttctgtct ttctgccgtt ttcaaatgca agggcggcaa tctggtcatt
      181 gcctgcgtca ccgattgtac gcttgccctt taccgaaatt gtgattgact ttgctgtcat
      241 aagccttgaa ttcttcgcag ttagcaatat ttgtcgtggc ggattctgtt cctgccttgc
      301 caaccgcaaa ctgattttca tagcacggga atactcccga ttcaactttt gccataaaat
      361 tacttccttt cgtaataaaa tttaacttca atgacctgct catacacacc cttgtcgtct
      421 gttcccacat caacgggttc ttccgtgagc agttcgatta tatagatttt gtgttcctta
      481 atttcaacat ttttaatgcc gtaaagcgtt tcgtaaagtc tgcgtgcaaa ctcctcggtt
      541 tctcttgcgt tgtcggtgta atggataagc aaagacacgc ttattgtatc gtaggtgctt
      601 tcaccgccga ttgcccttgt gggtgttccc gactgcttta atgaatacac accgattgac
      661 ctgtcctgct tgttgtcaag cttgccgatg taataatgct cggctgaggt aacgcttttg
      721 agccaatctc tgatgtccga taagtaaatc aaagtcctgc ttcctttctg tataatctca
      781 caaatgcccg actgcaaaaa ttctgccgtg taccgccctc aagccacggt gagaaccatt
      841 taccgccggc ggcaatgttt tccttacggc tgaaattata ctcgggatga aaatacaacc
      901 gccttgcata cggagtgctt gacacgattt taaccgtgcc gttccaactc tgcacacaat
      961 cttcaaaggt attttcgttc tgaagattac ccgtatcaaa cggcattacc tgcgtgtttt
     1021 tcacctgttt aagaagtgcg tcacctgtct gttcaagagc ctgttgcttt gccctatcaa
     1081 gctgttttac aacaggcata ttgagtttga tttttgatga taccgaaaat cccattaaat
     1141 cacatccaat tccgtaaaat taactttgcc gtcggggttg cggtgttttg taccctgtac
     1201 gatgtttcgt tttacgccgt caaggattac aaagccaccg cttaaagtgg ggctgtcggg
     1261 agcaatatcg ccgtcaaaaa gcaagacagc cgacacctga acaattttct gctctttggt
     1321 atagaccgtc tttgcctttg actgcatatt acacaaggca gagccaccgt gcagggttgc
     1381 tgacgggtac aagctgtcgg agggatacag atttttgcat tcaaacacgg tcaggggtgc
     1441 tccgtcttcg gtaacaccct caccgtagat tgtgacctcg acaggagttt tgcagaactg
     1501 cttttttaca agtgacggaa atttcacggt tttcacgcac ctttcagatt gcaggataac
     1561 aaagtcctgt tgattttagc aacgcataga ggtcggcagg aattgccact ccgctgatgc
     1621 acattaaatt ccagcttgcg ccaaattcca ttgatgtacc gttgattgaa tagcttttca
     1681 gataggaaga aatcatatcg gcattttctt cttcaaaagc agtaagtctg ctatgcactc
     1741 tgccgatgat tctcttctgc atttccgaaa gtttttcaaa atcaatgcgg ttaaaagtca
     1801 gaacatcaat gtgttcggca gagataatac tgttttcatc tccaccctga tgttcaatgt
     1861 aatcggcata cattacgcaa ccgccgttgt gtcaacatcg gcataaatgc tgtcaatttt
     1921 gccgtccttg ccgttcggga atacgaatgt gtcggaaagc gaacggttct gatagagcca
     1981 gccgtcaccc tctgtgtgtg agccgggagc aaagaagtaa atgcttgaaa tcttcggaac
     2041 agtcttgcag gtttcaccgc aagcaacaag aacattgatt ttgtgagcgc ctgttgcagg
     2101 ctcaaaaccg ccgtcatcgg ggttaaagtt gaagttatcg tagaaacgct catcgtcaat
     2161 aacctcgatg atagggcaac cgtcaatctc ggtcactctt gtttcaatgc cgataccgcc
     2221 ctctgcaatc tgtgtaagct caatcttacg agtgaactct gttgactgtt caaggcagtc
     2281 cataatgtga gatgtcacat aggcaacaag tgtgcctctt gccttgtatc tgcggagctt
     2341 gccggcagag agaattgttt tgagctttga ataagcgttc tccttagtcc actccgatgt
     2401 ctttgttgaa gaatggtagc cgtctgttgc ctgagccttt gctgcaacct ttgagaagaa
     2461 aagtgcgtct gtttcgggag caacctgtgt ctgctcaaac acctttgaaa tattctcaac
     2521 ctttgcggtt gcgttagttt cgtcaacatc tgccttatcc acaaggaact caatatctct
     2581 gtcgtgctcg caagtgaaag gaacatctgt ctgtgtatat ttgcctttgt tccaaccgcc
     2641 ctctctgctg tggttcttaa agcctgttgt tgacatctgt gtaaagtgga atgttcttgc
     2701 accc
//
LOCUS       DAOMVE010000188         2622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_11628_length_2622_cov_3.008570, whole genome shotgun sequence.
ACCESSION   DAOMVE010000188 DAOMVE010000000
VERSION     DAOMVE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11628_length_2622_cov_3.008570"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLS64_08580"
     CDS             complement(<1..317)
                     /locus_tag="PLS64_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRL43910.1"
                     /translation="MSEFIEKLKSRRSVRSFKSEMPPKEVIDEIISAGLYAPSGMNRQ
                     PVIIVAVTNKEMRDRLSRMNAEIMGKDVDPFYNAPVVLIVLADKAAPTYLYDGTLVME
                     NLML"
     gene            complement(330..869)
                     /locus_tag="PLS64_08585"
     CDS             complement(330..869)
                     /locus_tag="PLS64_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRL43911.1"
                     /translation="MELSEALNNRKSTRHYIDKKVDKSLLDEMLSAANLAPSWKNSQT
                     PRFYVATGEKSKNAVREALPEFNRKNSENASAYIVTTVVLNRSGFDKDGTAVTEIGNG
                     WGYYDLGLNNMALLLKATELGISTLVMGIRDGEKLRKEFDIPETEAVVSVIAVGYTDS
                     EIVRPKRKSVEDFAKFYED"
     gene            complement(931..1857)
                     /locus_tag="PLS64_08590"
     CDS             complement(931..1857)
                     /locus_tag="PLS64_08590"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL43912.1"
                     /translation="MEIFSSVIFQIAVLFSFIFIGFTIRQGGFLPENSAEIFSKLENR
                     ILMPAVVINTFRTNCTVKNISEKWVFIAYSTGVLLFCIATGFVASRFLGKTEYLKKVY
                     RYSISVSNFGFVGTAMVSGIYGAESMELFDYLMFTLPLNLFTYSIGIAWLVPNGHGKF
                     SMKNFVNPIFVSIFIGAILGLLPIPKFRLVTTIINSTAACMSPIAMILTGFVIGGYSF
                     ANLFGKWQTYVVAGIRLVFLPTVSVLVLKLFNAPQEAVVATLCANAMPLGLNTVIIPS
                     AYGKSPDDGASMALVSQLTAVFTIPILFYILL"
     gene            complement(1927..2109)
                     /locus_tag="PLS64_08595"
     CDS             complement(1927..2109)
                     /locus_tag="PLS64_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HRL43913.1"
                     /translation="MENNAYCNQSIMCSVTNCKHHNSTKDYCSLEAIKVGTHEANPTV
                     CQCTDCQSFEAKCSCK"
     gene            2230..2562
                     /locus_tag="PLS64_08600"
     CDS             2230..2562
                     /locus_tag="PLS64_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43914.1"
                     /translation="MNVVSAVFLVVFAVIGICAFVRELTFFLFRNRKNNTVMFVAPVS
                     GKCENAEYILRSAAAKVKWISRGRNDCVICLDCGMDDETKRICQKICRDYGFAKLLTK
                     QEFLKMIK"
BASE COUNT          783 a          554 c          509 g          776 t
ORIGIN      
        1 agcataagat tttccattac aagagtaccg tcatacagat atgtaggcgc tgctttatcg
       61 gcaagcacaa tcagaacaac gggagcgtta tagaacggat caacatcttt tcccataatt
      121 tcggcattca ttcttgaaag cctgtcacgc atttctttgt ttgtaacggc aacgataata
      181 accggctgtc tgttcattcc tgacggtgca tacagacctg cggaaataat ttcgtcaata
      241 acttcctttg gcggcatttc agacttaaag ctgcgaacgc ttcttcttga tttcagtttt
      301 tcgataaatt cactcattat gtaaacctct cagtcctcat aaaattttgc aaaatcctca
      361 acagattttc tcttcggtct tacgatttcg gaatcagtgt aaccaactgc gataacgctg
      421 acaacagcct ctgtttcggg aatgtcaaat tcttttctga gcttttcacc gtcacggata
      481 cccataacaa gggttgaaat gccgagttcg gttgccttta gcaaaagtgc catattgtta
      541 aggccgagat cgtaatatcc ccagccgttg ccgatttcag tcacggcagt accgtctttg
      601 tcaaaacccg aacggttgag aacaactgtt gtcacgatat atgcagacgc attttctgaa
      661 ttttttctgt taaattcggg gagtgcctct cttacggcat tcttactttt ctcgcctgtt
      721 gcaacataaa agcgcggcgt ctgcgagttc ttccacgaag gagcaagatt tgccgccgac
      781 agcatttcat caagcaagct tttgtcaact tttttatcta tataatgtct tgtgcttttt
      841 ctgttattta aagcctcaga taattccata atcaaacctc cgatattttt atacctatat
      901 tgtaccaatg gagtgggaat taatcaaggg ttaaagcaga atatagaaca aaatcggtat
      961 cgtaaaaact gccgtaagct gtgacacaag cgccatactt gcaccgtcat cgggactttt
     1021 tccgtaggct gacggaatga ttaccgtgtt aagtccaagc ggcattgcat ttgcgcaaag
     1081 tgttgcaaca actgcctcct gaggtgcgtt aaaaagcttt aacacaagca ccgaaaccgt
     1141 tggcagaaac acaaggcgga ttcctgcaac aacataggtc tgccatttgc cgaaaagatt
     1201 tgcaaagctg tagccgccta ttacaaaacc tgtcagaatc attgcaatgg gcgacataca
     1261 ggcggcggtt gaattgatta ttgttgtaac aagtctgaat tttggaatcg gcaataaacc
     1321 gagaattgct ccgataaaaa ttgaaacaaa aatcggatta acaaaatttt tcattgaaaa
     1381 tttgccgtgt ccgttcggca caagccacgc aattcctatt gaataagtaa acagattaag
     1441 cggtaaagtg aacataagat agtcgaaaag ctccatgctt tctgcgccgt atattcccga
     1501 caccattgca gttccgacaa atccgaaatt tgaaacggaa attgaatagc ggtacacctt
     1561 tttcagatat tccgtcttac caagaaatct tgacgcaaca aatcccgttg caatgcaaaa
     1621 cagcagaacc cccgtgctgt aggcaatgaa cacccatttt tcgcttatgt tttttactgt
     1681 acaattggtg cggaatgtgt taattacaac ggcaggcata agaattctgt tctcaagctt
     1741 tgaaaaaatt tcggctgaat tttccggcaa aaatccgccc tgtcggattg taaaaccgat
     1801 aaatataaac gaaaacaaaa ccgcaatctg aaaaattacc gatgaaaaaa tctccacaaa
     1861 ttcatcccct tttaaagcca aaacaccccc agtccgaggg gctgagggtg taagcttcaa
     1921 atcaaattac ttgcagctgc actttgcttc aaagctctgg cagtccgtac actgacaaac
     1981 agtcggattt gcctcgtgag ttccgacctt gattgcttca agcgaacagt agtcttttgt
     2041 gctgttgtga tgcttacagt ttgttaccga gcacataatg ctttggttac aatatgcatt
     2101 gttttccata gtattcattc tcctcattca tttttgcgga ttagccgctt tttatattgt
     2161 ttctgcattt tgcggattta taccgcacac tttgaaacat ctgcaataaa atgtattgag
     2221 gtgaattaca tgaatgttgt atcggctgtt tttcttgttg tttttgccgt aatcggaatt
     2281 tgtgcatttg tccgtgaatt aacatttttt ctctttcgta acagaaaaaa taataccgtt
     2341 atgtttgttg ctcccgtcag cggaaaatgt gaaaatgccg aatatatttt gcgaagtgct
     2401 gccgcaaagg taaaatggat aagcagagga agaaatgact gtgtaatttg ccttgactgc
     2461 ggtatggacg atgaaacaaa aaggatttgc caaaaaatat gtcgtgacta cggctttgca
     2521 aagcttttga caaaacagga atttttaaaa atgataaagt aaaaatccaa acgaaattta
     2581 aacaaaaata ctgccacaga atatgcccag gttttataaa gc
//
LOCUS       DAOMVE010000189         2601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_11739_length_2601_cov_4.493716, whole genome shotgun sequence.
ACCESSION   DAOMVE010000189 DAOMVE010000000
VERSION     DAOMVE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11739_length_2601_cov_4.493716"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..1256)
                     /locus_tag="PLS64_08605"
     CDS             complement(228..1256)
                     /locus_tag="PLS64_08605"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HRL43915.1"
                     /translation="MAVSVHKWLLNKFRDINHSRMDKHIDIIAKNSGKSKAYIKFDII
                     RNFLIRGTGYTDYFRCDFINLSAKEKKTFVTAKTFYKILEYLNDEEYIVLLRDKLVFD
                     ELFKKYLKRDFINLRTGSKEDFRKFLDGRETVFAKDPTGEGGHGISKITVADVKDSNK
                     LYDELKANGQLLVEEAIVQSDDLNEINPCVVNSWRVVTLYKDGKAHIINNALRINQDE
                     SNVIGCTNDLYLSLDADGRIDSNVIDDYGNVYDKHPMTGKKFSEVKIAGVREALDMVV
                     EAAEKIPQVRYIGWDIAFSKNGPVMVEGNEYPGYGLLQFYKLKGKRTGHLKEIADVLG
                     DEMNNIKL"
     gene            complement(1258..2442)
                     /locus_tag="PLS64_08610"
     CDS             complement(1258..2442)
                     /locus_tag="PLS64_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRL43916.1"
                     /translation="MLDSYVEIDLDKIGANARAITQKYSDYKYFIAVLKGDGYGHGME
                     VANEMYKNGINYFAVSSLEEAQSFRKVNKDAPLLCLHPVHLSRIEEAKRLNLTIAVNE
                     LDYLKRLLDLNIDCNFKVHLQIDTGFNRLGFKDKNEVKTAVDMINGSNFVLEGIYQHF
                     ATAGIFDPHWDEQVRNFKELTSLIDLNKIPIVHMGSSVSMLTHPKQPFTTGVRMGIAI
                     YGYNVAPDSLSNSPKDKLRKMRNNYFIKKYNISETYFDVKIDLQPAMKMKTRILQLKK
                     VNKGEWFGYNASYTADEDIILAILPVGYNNGIGHANHNRQVVINGKKYPVVGEMCMNM
                     TAVKVDSSVKIDDEVTLLGDGITVGMFGRSSGMGNAEALVNIGKNNPRFYVKDGKVVK
                     EK"
BASE COUNT          698 a          573 c          522 g          808 t
ORIGIN      
        1 ttttcgcaca ttttacaaag cgtggaatgc cacgacagaa ttacggttat tttgtgatat
       61 ttatattatg atttaatcta aattattgtg tctttatttg cgggcggctg atgaccgccc
      121 atacaggatt acgcaacctc acacaataca tcccatacaa cgcacaacag caccgatatt
      181 acttcgatgc tgttttttgt ttttgttata aagtttttgc tgaataatca aagcttaatg
      241 ttattcatct cgtcaccgag gacatcggcg atttctttca ggtggcctgt gcgtttgcct
      301 tttagtttat agaactgcaa aagtccgtag cccgggtact cgttgccctc aaccataacg
      361 ggaccgttct ttgaaaaggc gatatcccaa ccgatatatc tcacctgcgg aatcttttcc
      421 gctgcctcaa caaccatgtc aagagcctct cgaacgcctg caatcttaac ctcggagaac
      481 tttttgcctg tcatcgggtg tttgtcataa acattgccgt agtcgtcaat tacattactg
      541 tcgattctgc cgtctgcatc aaggctgagg tagaggtcat ttgtacagcc gataacattt
      601 gactcgtcct gattgatacg gagagcgtta tttattatat gtgccttgcc gtccttatag
      661 agcgtaacaa ctcgccatga atttacaacg cacggattga tttcgttgag gtcatcgctc
      721 tgaacgattg cttcctcaac aagaagctga ccgtttgcct tgagttcatc ataaagcttg
      781 ttgctgtcct ttacatcggc aacggtgatt tttgaaatgc cgtgaccgcc ctcgcctgtc
      841 ggatcttttg caaaaacggt ttctctgccg tcaaggaact tcctgaaatc ttccttactg
      901 cctgtgcgaa ggttgatgaa atctcttttg agatactttt tgaaaagctc gtcaaaaaca
      961 agcttgtcac gaagaagaac gatatattcc tcatcgttga gatattcaag aattttgtag
     1021 aaagtttttg cagttacaaa ggtttttttc tctttagccg aaaggttgat aaaatcgcaa
     1081 cggaaatagt ccgtgtagcc tgtgcctctg atgagaaagt tgcggattat gtcgaacttg
     1141 atataagcct ttgacttacc gctgttcttg gcgataatgt caatgtgctt atccattctg
     1201 ctgtggttaa tatctctgaa tttattcaaa agccatttgt gtaccgaaac tgccatctta
     1261 cttctccttt acaaccttac cgtcttttac ataaaatctc ggattgttct tgcctatatt
     1321 cacaagcgcc tcggcattgc ccatacccga acttctgccg aacattccaa cggttatgcc
     1381 gtcaccgagg agtgttacct catcatcaat ttttaccgat gaatcaacct taacagccgt
     1441 catattcata cacatctcac cgacaacagg atattttttg ccgttgataa caacctgtct
     1501 gttgtgattt gcgtgaccga tgccgttgtt gtagccgaca ggaagaattg caagaataat
     1561 atcctcatct gccgtgtatg acgcattgta gccgaaccac tcgcccttgt tcaccttttt
     1621 aagctgaaga atacgggttt tcatcttcat agccggctgt aaatctatct ttacatcaaa
     1681 atatgtttcg ctgatgttgt acttcttaat gaagtaattg tttcgcattt tgcgaagttt
     1741 gtcctttggc gaatttgaaa gagaatcggg ggcaacattg tagccgtaga ttgcaatgcc
     1801 cattctcacg cctgttgtaa aaggctgttt cgggtgggtg agcattgaaa cggaactgcc
     1861 catatggaca atcgggattt tattcaaatc aataagcgat gtaagctcct tgaagtttct
     1921 gacctgctcg tcccaatggg gatcaaaaat tccggctgtt gcaaaatgct gataaattcc
     1981 ctcaagaaca aaatttgaac cgttaatcat atcaacggca gttttaacct cgttcttatc
     2041 cttgaagccg aggcggttaa atcctgtatc aatctggaga tgaaccttga aattgcagtc
     2101 aatgttcaaa tcaagcaacc ttttgaggta gtcaagctca ttcacggcaa tggtgagatt
     2161 aagccgcttt gcctcctcaa ttctcgaaag atgaaccgga tgcaggcaca aaagcggagc
     2221 gtccttgttg accttacgaa agctttgagc ctcctcaaga gaggacacgg caaaatagtt
     2281 aatgccgttt ttgtacattt catttgccac ttccataccg tgaccgtaac cgtcaccctt
     2341 gagaacggca ataaaatatt tgtagtcgct gtatttttgt gttatcgcac gggcatttgc
     2401 accgattttg tcaaggtcaa tttcaacata tgaatccagc attccaaagc ctccgttaat
     2461 acactatatc cttagaatta tagcataatt gcatgggtaa atcaatagcg atattgaaca
     2521 gtaacggtgt acggttcggg cataaaattg aaaattgaaa atgtaaaatg gaaaattatg
     2581 gtcgggaaga caatttgcaa g
//
LOCUS       DAOMVE010000190         2574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_11899_length_2574_cov_3.992457, whole genome shotgun sequence.
ACCESSION   DAOMVE010000190 DAOMVE010000000
VERSION     DAOMVE010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11899_length_2574_cov_3.992457"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLS64_08615"
     CDS             complement(<1..775)
                     /locus_tag="PLS64_08615"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRL43917.1"
                     /translation="MARSAGVLLSITSLPSPYGIGTIGKEARKFADFLKKSGQTIWQI
                     LPVGPTSYGDSPYQSFSTYAGNPYLIDLDTLCEEGLLTKEEVMSRDWGSDDAEVDYEK
                     IYNNRFEVLKIAYDNFKKGDQKAFTSFKRKNSSWLKNYALYMAVKKSFDMVSWTEWPD
                     EEIKMRDEAAVKRYERKLKDDVDFWKFVQFKFYEQWESFRAYVNGLGIKILGDMPIYV
                     AMDSADTWANPELFQLYDDGDPIAVAGCPPDYFSATGQLW"
     gene            complement(917..1462)
                     /locus_tag="PLS64_08620"
     CDS             complement(917..1462)
                     /locus_tag="PLS64_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HRL43918.1"
                     /translation="MITLTDTDVVRLLRKNNLNSDTLQENVVAKLREKGVKLATAESC
                     TGGLLSERITAVSGASDVFDFGVCTYANEMKHKVLGVSNETLSVLGAVSAETAMQMAE
                     GARNVSGADLAVSTTGIAGPTGGTPEKPVGLVYCGISTKDKSYAIKLMLGGSINMVNS
                     RGYIRKLTSDAVLLTILSILQ"
     gene            1602..2330
                     /locus_tag="PLS64_08625"
     CDS             1602..2330
                     /locus_tag="PLS64_08625"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRL43919.1"
                     /translation="MAWFFTEKNIFGNNYTLVGENAKHISKVLRMRTGDDITLVSPDK
                     TQYECKISLITQGEVTVDIISQKPCENEPDVFVTLYQALPKGDKMDFIVQKCTELGIS
                     RIVPMISARCVSRPDEKSLKKKCERWQKIALQAAMQSRRGIIPEVTPCISFKEATEQT
                     KQNEKTVFFYEMGGESVRKILNGTKPKTVGMFIGSEGGFEQSEVDSVTAIGGQAATLG
                     KRILRAETAPLAALSIIMYETDNF"
     gene            complement(2327..>2574)
                     /locus_tag="PLS64_08630"
     CDS             complement(2327..>2574)
                     /locus_tag="PLS64_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43920.1"
                     /translation="CCALHKLSSREGCEALAYESVYATAMLRSGIICCALHKLSSREG
                     CEALAYESVYATAMLRSGIICCALHKLSSREGCEALA"
BASE COUNT          737 a          620 c          535 g          682 t
ORIGIN      
        1 cccaaagctg acctgttgcc gagaagtaat ccggcggaca gcctgcaact gcgatgggat
       61 caccgtcgtc ataaagctga aacagctcgg gatttgccca tgtgtctgcg ctgtccatgg
      121 caacatagat tggcatatcg cccaaaatct tgatgccgag accgtttaca tacgcacgga
      181 aggattccca ctgctcatag aatttgaact gtacaaactt ccagaaatca acatcatcct
      241 tgagcttgcg ttcataacgc ttcacggcag cctcatcacg cattttgatt tcctcatccg
      301 gccattctgt ccaggaaacc atgtcaaagc tctttttaac tgccatataa agtgcatagt
      361 ttttgagcca tgaactgttt ttacgcttga aggatgtaaa tgccttctgg tcaccctttt
      421 tgaagttatc gtaagcaatc ttcaaaactt caaaacggtt gttgtagatt ttctcatagt
      481 caacctcggc gtcatctgag ccccagtcac ggctcatgac ctcttcctta gtcaaaagac
      541 cttcttcaca aagagtgtca aggtcaatga ggtaaggatt gcctgcatag gttgaaaaag
      601 actgatacgg tgaatcaccg taacttgtcg gtccaacggg gagaatctgc cagattgtct
      661 gacctgattt tttcagaaag tcagcaaact tgcgagcctc tttgccgatg gtgccgatac
      721 cgtaggggga tggaagcgaa gtaatcgaaa ggagaacacc tgcgcttcta gccataaaaa
      781 tcaactccat tctgacggac tgttaaattt tttaaatctg cgaattcggg cgcaattcgc
      841 ccctgaaccc acagtatcaa gataatttta gcacataatc taaattttta caagttattt
      901 ttttaacaac gacaagttat tgcaatatac tcaaaatagt cagtaaaaca gcgtcagaag
      961 ttagttttct tatatagcct cttgaattta ccatgttaat actgccgcca agcataagct
     1021 ttattgcata acttttgtct tttgtgctga ttccgcaata aacaagaccg acaggctttt
     1081 cgggagttcc gcccgtagga cctgcaattc ccgttgtact tacggctaaa tccgcacccg
     1141 aaacatttct tgcaccctct gccatctgca ttgcagtttc agccgaaact gcgccaagca
     1201 cagacagcgt ttcgtttgaa actccaagca ccttatgttt catttcattt gcatatgtgc
     1261 atacgccaaa atcaaacaca tcgcttgcac cgcttactgc ggtaattctt tcgcttaaaa
     1321 gaccgcccgt acagctttct gcagttgcaa gctttacacc cttttcacgg agctttgcaa
     1381 caacattttc ctggagcgtg tcggaattga gattgttttt tctcaaaagc cgaaccacat
     1441 ctgtatctgt cagagttatc attaataatc atctccgaaa atttttcgta tatccatatt
     1501 ttagcaccgg catatgaata ttgcaattat ttttattttt tgatatatta aatagaaaag
     1561 gaaagcgctc acgcaaaaac gcttaaaaaa gaggtaattt tatggcttgg ttttttaccg
     1621 aaaaaaatat attcggcaac aactacaccc ttgtcggaga aaacgcaaag catatttcta
     1681 aggtgctgcg tatgcgtaca ggcgatgaca tcacccttgt ttctccggac aaaacgcagt
     1741 atgaatgtaa aatttcgctc atcacacagg gtgaagtcac ggttgacatc atctcacaaa
     1801 agccgtgtga aaacgaaccc gatgtttttg taaccctcta tcaggctctg cctaagggtg
     1861 ataaaatgga tttcatcgtt cagaaatgca cggaactcgg aatcagcaga attgtgccga
     1921 tgatttcggc aagatgtgtt tcccgtcccg atgaaaagtc gctgaaaaag aagtgtgaac
     1981 gctggcagaa aattgctctt caggcggcaa tgcagagtcg caggggcatt atccccgaag
     2041 taactccgtg catttcgttt aaagaggcaa cagaacagac aaagcagaac gaaaagaccg
     2101 tattttttta cgaaatgggc ggagaatccg tcagaaaaat tctgaatggc acaaagccta
     2161 aaaccgtcgg aatgttcatc ggcagtgaag gcggatttga acaatcggag gttgacagcg
     2221 taaccgcaat cggcggtcag gcggcaaccc tcggcaaacg aattctccgt gcagaaaccg
     2281 cccccctcgc cgccctatcc attattatgt acgaaaccga caatttttag gcgagtgcct
     2341 cgcacccctc tcgtgaagac agtttgtgca atgcacaaca aatgatgccc gagcgcagca
     2401 tagcagttgc atataccgac tcgtaggcga gtgcctcgca cccctctcgt gaagacagtt
     2461 tgtgcaatgc acaacaaatg atgcccgagc gcagcatagc agttgcatat accgactcgt
     2521 aggcgagtgc ctcgcacccc tctcgtgaag acagtttgtg caatgcacaa caaa
//
LOCUS       DAOMVE010000191         2557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_12032_length_2557_cov_2.300160, whole genome shotgun sequence.
ACCESSION   DAOMVE010000191 DAOMVE010000000
VERSION     DAOMVE010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12032_length_2557_cov_2.300160"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="PLS64_08635"
     CDS             complement(<1..1243)
                     /locus_tag="PLS64_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRL43921.1"
                     /translation="MYSTIKSIQLLTALLKKYNINKLVLSPGGSDIPIIHSLENDDFF
                     ECHSVVDERSAVYYGIGLAQQTKSPVACICTSGTAVSNYLPGMTEAFYQDVPIVAITA
                     DKDPYRLNQLMLQKTQQVNIFESVTKKSVNLPVVRGGNDRWYCERLINEALSELDHHG
                     TGPVHINIPIVENSAVYDCENLPQVRKINRISPDMPSEKWREYAERLSKYKKILVIAG
                     QNNCFSEDDRACVEKFFEKYNCLISVEHMSNLKCRGCLMTYPLSECSMQGMFGELCPD
                     LIISFGNNIASYKLKPMIKAHKEKFVHWQIDTAGRIRDFSDKLTDVFECTPQYFFNCF
                     AENAPQNSKNDMTYYNMWNDDIKMLDYSSNLPFSNMYVAKKLAEIIPDRSVLHLAILS
                     STRTMQFFDLKPNVKVYSNIGA"
     gene            complement(1259..1417)
                     /locus_tag="PLS64_08640"
     CDS             complement(1259..1417)
                     /locus_tag="PLS64_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43922.1"
                     /translation="MSLKNTLKKVRQGIIKTFKAKNVVPVYVQKENNNILDGKTALIT
                     GGSGIIKN"
     gene            complement(1419..2498)
                     /locus_tag="PLS64_08645"
     CDS             complement(1419..2498)
                     /locus_tag="PLS64_08645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HRL43923.1"
                     /translation="MKKVGIVTFHRALNYGAVLQSYALQKTVSSLGAECEIVDYICPK
                     ITADYKPFRIYKNDLLKSFAKSCVMVRRRAKRRDAFKSFFNNYLVKSKKSYTPQNIGE
                     LKNDYDLFISGSDQVWSPRCVGFDPAYFLTFADDKQKYSYAASFAVPTLPDEFIDEYK
                     KRLNGFQKFSVREPSGEKLVKELTGRTAETHLDPTLLISADEWKKIAVSKIDKPYILI
                     FTANPAVSLVDFALKLSKEKNLPVYCINDAPHPVSHGINYIVAPTVEEFVGCFKNAEY
                     VVTNSFHGTAFSVIFNKNLFVEFKNKSGRNIRSEGLLKSLGIDREIVDGNCRETEINW
                     YDVNEKIKNQTIISLDYLKSFVSGG"
BASE COUNT          713 a          528 c          512 g          804 t
ORIGIN      
        1 gagcgccgat attgctgtaa accttgacat tcggtttcaa atcgaagaac tgcatagttc
       61 ttgtactgct gagaattgca aggtgcagaa cagaacggtc gggaataatt tcagcaagct
      121 ttttggcaac atacatattt gagaacggaa gattgcttga ataatcaagc attttgatat
      181 cgtcattcca catattgtag tatgtcatat cattttttga attctgcggt gcgttctctg
      241 caaagcagtt aaagaaatac tgcggtgtac attcaaatac atctgtcagc ttgtcggaaa
      301 aatctctgat tcttcccgca gtatcaattt gccagtgaac aaatttttcc ttgtgagcct
      361 taatcatagg cttgagttta tatgaagcaa tattgttgcc gaacgaaata ataagatcgg
      421 ggcataactc gccgaacatt ccctgcattg agcattctga caaaggatag gtcataaggc
      481 agcccctgca cttgaggttt gacatatgct caaccgatat aaggcaattg tatttctcaa
      541 agaacttctc gacgcacgct ctgtcatctt ccgagaaaca gttgttctga ccggcaatga
      601 caagaatttt cttatatttt gaaagccttt ctgcatattc acgccacttc tcggagggca
      661 tatcgggaga aattctgttg attttcctaa cctgaggaag attttcacaa tcatatacag
      721 ccgagttttc aacaatcgga atattgatat gcacaggtcc tgttccgtga tggtcaagct
      781 ccgaaagagc ctcgttaata agtctttcgc agtaccatct gtcatttccg ccacgcacaa
      841 cgggaagatt aacggatttt tttgtaaccg attcaaaaat attgacctgc tgggttttct
      901 gcagcataag ctgatttaat ctgtacgggt ctttgtcagc ggtaattgcg acaatcggaa
      961 catcctgata aaatgcctct gtcatacccg gaagatagtt gcttacagcc gtacccgagg
     1021 tacagataca ggcaacgggg gattttgtct gttgtgcaag tccgatgccg taatacacgg
     1081 cacttctctc gtcaacgacc gaatgacact caaaaaaatc gtcattttca agtgaatgaa
     1141 ttataggtat atcactgcct ccgggagaaa gcaccaattt gttgatatta tattttttca
     1201 gaagtgcagt gagcaactga atgcttttta ttgtagaata catacgcttt cctccgcttt
     1261 agtttttgat aattccggaa ccgcctgtaa tcagagcggt tttgccgtca agaatattgt
     1321 tattctcctt ttgtacatac acgggaacaa cattttttgc tttaaatgtt ttgattatgc
     1381 cctgtctgac tttttttagt gtatttttaa gactcatttt aaccaccgct gacaaaggat
     1441 ttaagataat ccaaagatat tattgtctgg ttcttgattt tttcgttcac atcgtaccag
     1501 tttatttcgg tttctcggca gtttccgtca acaatttcac ggtcgattcc aagacttttc
     1561 aaaaggcctt cggagcggat gtttcttccg cttttgttct tgaattcaac aaaaagattc
     1621 ttgttgaaaa taacagaaaa cgctgttccg tgaaacgagt ttgtaacaac atattcggca
     1681 tttttgaaac agccgacaaa ttcctcaaca gtaggtgcaa caatataatt tataccgtga
     1741 cttacagggt gaggtgcatc gtttatgcag taaacgggaa ggttcttttc ttttgacagt
     1801 ttaagggcaa aatccacaag tgaaactgcc ggattagccg taaaaatgag gatatacggt
     1861 ttgtcgattt ttgaaacggc aatctttttc cattcgtctg ccgaaatcag cagggtagga
     1921 tcaagatgag tttcggcggt tctgcccgtg agttctttta caagcttttc tccgctcggt
     1981 tcacgaactg agaacttctg aaaaccgttc agcctttttt tatattcatc aataaattca
     2041 tcgggcaatg tcggaacggc aaagcttgcc gcatatgaat acttctgttt gtcatcggca
     2101 aatgtcagaa aataagcggg atcaaaaccg acgcatctcg gactccacac ctgatcgctt
     2161 cccgaaatga acaagtcata atcgttttta agctccccga tattctgcgg agtatacgac
     2221 tttttactct ttacaagata gttattgaaa aacgacttaa aagcgtctct gcgttttgct
     2281 cttcttctta ccatgacaca gctttttgca aaggatttca gaaggtcgtt tttataaatg
     2341 cgaaagggtt tgtagtcggc tgtgattttc ggacagatat agtcaacaat ttcacactcc
     2401 gcaccgagcg aggaaacggt tttttgcaac gcatagctct gaagcacagc gccgtagttc
     2461 aatgcacggt gaaatgttac aatgcctacc tttttcatta tctgcttccc cctgattgtt
     2521 ttttgtacaa aaaatatccc atgggataaa atatgat
//
LOCUS       DAOMVE010000192         2500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_12414_length_2500_cov_3.469530, whole genome shotgun sequence.
ACCESSION   DAOMVE010000192 DAOMVE010000000
VERSION     DAOMVE010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12414_length_2500_cov_3.469530"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(429..1616)
                     /locus_tag="PLS64_08650"
     CDS             complement(429..1616)
                     /locus_tag="PLS64_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRL43924.1"
                     /translation="MKKKIFILLLCVVLIATCILCGVTGIFGNDSQNSPKPQTPDTAV
                     TENMTFPSKVDSLTLKVGESKLIELSSEKAKSVTKWTSSDSKIVTVDDGGRVDALKEG
                     TALISAISKDKSKSEFQVTVAKSTTKKQQSYSTCITANLDKLENNKRNTAKNLYAIKV
                     NRTANCVTVYTYDEKGKYTIPVRAMICSTGLDNSTITGDYTIGIKSEWLFLVGDVFGR
                     YISGISGDYLFHSVPYYSMSEEDLELEEFNKLGEQASQGCVRLAVSDAKWVYDNCPTG
                     TNVSIYDDAENAGPLGKPDAIKITDFTNKWDPTDSNKKCPYAKATPIISGANDYTIKS
                     GGEFYALAGVTAVDTCGNDITSNIEVFGNVVTTRKGKYKVTYSVTDVLKRTSSVTITV
                     TVQ"
     gene            1793..2317
                     /locus_tag="PLS64_08655"
     CDS             1793..2317
                     /locus_tag="PLS64_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HRL43925.1"
                     /translation="MAHPIKHFRTITRHRHKVIANCARAGILRQGLIHDLSKYSPTEF
                     ILGAKYFQGTRSPNEKERELYGYSQAWMHHKGRNRHHYEYWNDYNPKTKQIENVEMPV
                     KYVIEMFCDRVAASKIYNGKNYTDGAPFTYFCRIRGKHRMHPNTEALLEKLLVMLRDE
                     GEEKTFAYIRSMKK"
BASE COUNT          677 a          511 c          530 g          782 t
ORIGIN      
        1 aattgaaaat gtaaaatggg aaattgaaaa ttatggtcag cggtatgcca tcgcagccct
       61 ttcgggaaga cagtcttgtt aatgagagag tgaatatgcc cgagtgtagg tatgcaatgt
      121 ttgtacaaat ctgtatgggc gaacacggtt catccataca ttattacagt tgctttgcag
      181 catttataaa atttgtatta atatgtagtc tgtaccgttg cttgcgggcg aatgatatcc
      241 gccccaacag ataaacaaca actttcatat ataaaaaaca gtcgggaaga cgatgttttg
      301 cattttgcaa accgtcttcc cgacattcat tttccatttt caattttcca ttttcaattt
      361 aaaatctccg ttcgggcatc tttattttcg cttttgcgga actatcttct taaattgact
      421 gcggcggttt attgcaccgt taccgtaatc gttacgcttg atgttctttt aagcacatcg
      481 gtgactgaat atgttacctt gtacttgcct tttcgggtgg ttactacatt gccgaaaact
      541 tcgatattcg atgttatgtc gttgccgcag gtgtcaacgg ctgttactcc tgcaagggcg
      601 taaaattcgc cgccgctttt gattgtgtaa tcgtttgcac cgctgattat tggtgttgct
      661 ttggcatacg ggcatttttt attgctgtct gtcggatccc atttgttggt aaaatcggtg
      721 attttgattg catccggttt tccgagaggg cctgcatttt ctgcgtcgtc atatatgctt
      781 acattcgtgc ctgtggggca gttgtcataa acccactttg catccgaaac cgcaagtctt
      841 acgcatcctt gtgatgcctg ttcgccaagc ttgttgaact cctcaagctc caaatcttct
      901 tcgctcattg agtagtaggg aacggagtgg aaaagataat cgcccgaaat tccgctgatg
      961 tatcttccga aaacatcgcc cacaaggaaa agccattcgg acttgattcc gattgtgtag
     1021 tcacccgtga ttgtcgaatt gtcaagacct gtggagcaaa tcatcgccct tacggggatt
     1081 gtgtacttgc ccttttcgtc atatgtgtaa accgtcacgc agtttgccgt gcggttgacc
     1141 ttgattgcgt acaggttttt tgccgtgtta cgcttattgt tttcaagctt gtcaagattt
     1201 gccgttatac aggttgagta gctttgctgt tttttcgtgg ttgattttgc caccgtaacc
     1261 tgaaattccg actttgattt gtcttttgaa atggcagaaa tcagagccgt accctctttg
     1321 agagcgtcaa cccttccgcc gtcatcaacg gtaacaattt tactgtccga gcttgtccat
     1381 ttggtaacgg attttgcttt ttcggatgac agttcaataa gctttgattc gccgactttt
     1441 aatgtgagcg aatcgacctt gctcggaaat gtcatatttt cggttactgc cgtatcgggc
     1501 gtctgcggtt ttggagaatt ttgagaatca tttccgaaaa ttcccgtcac accgcataat
     1561 atgcaggttg caatgaggac aacacaaagt aaaagaatga aaattttctt tttcatagat
     1621 tacctatcct tatagaattt aacattaaat ggttacaaaa ttttaacctt tgtatattat
     1681 aacttttttt cgacaatctt gcaagtgcta ttttaaatcg gcaagaaatg tggtaaaatg
     1741 tcagtcggag aatgttttgt attttccaaa ctaacaattt atggtgataa ttatggcaca
     1801 tccgattaaa catttcagaa caataacccg tcacaggcac aaggtaattg ccaactgtgc
     1861 aagggcagga attctcagac agggattaat acacgatttg tcaaagtatt cgcctacaga
     1921 atttattctg ggtgcaaagt atttccaggg cacgagaagt cccaatgaaa aggaaagaga
     1981 actgtacggc tattcacagg cgtggatgca tcacaagggc agaaaccgtc accattacga
     2041 atactggaac gattacaacc caaagacaaa gcagattgaa aatgttgaaa tgcctgtaaa
     2101 atatgttatc gaaatgttct gcgaccgagt tgccgcaagc aaaatctaca acggcaaaaa
     2161 ttacacggac ggcgctccgt ttacatattt ttgcagaatc agaggcaaac accgtatgca
     2221 ccccaacacc gaggctttgc ttgaaaaatt attggttatg ctgagagatg agggtgaaga
     2281 aaagaccttt gcatatataa gaagtatgaa aaagtgattg tttgtgcgaa tcctcccgtc
     2341 tgaaatatag tcgcaatgcg ttgcacataa atgtgcttcc tattgctcgt tatttcagac
     2401 agcgcattgc tccctttttc cgccaccggc ggcggtcgca aggctaccaa tcaacacgaa
     2461 atgaataagt aatcgcagaa aatattttgt ttattaatat
//
LOCUS       DAOMVE010000193         2475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_12583_length_2475_cov_2.126033, whole genome shotgun sequence.
ACCESSION   DAOMVE010000193 DAOMVE010000000
VERSION     DAOMVE010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12583_length_2475_cov_2.126033"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..758)
                     /locus_tag="PLS64_08660"
     CDS             complement(144..758)
                     /locus_tag="PLS64_08660"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HRL43926.1"
                     /translation="MSDKPKCSKKANRSTEYIYKDKYVNGELISHKCIKQQVLTYPTD
                     KIIVKGNRNIDIINKSYNNKTSYLIKTKYDNKDFKLPMVKLSDKDRDMLERIVTGEFG
                     GSYVGSCLIAQSIKCAIVYDGYTSVSAVIKGMGYVGSTANRSQNAVNAVKYIFDDNNL
                     AVRHRLFYMCTKDYYDSTPGNFHSTQSFILQYENVLFFDRWQKK"
     gene            complement(700..1155)
                     /locus_tag="PLS64_08665"
     CDS             complement(700..1155)
                     /locus_tag="PLS64_08665"
                     /inference="COORDINATES: protein motif:HMM:NF015923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HRL43927.1"
                     /translation="MNKFIHTTIKLSFLSVIISMTALLNTAPIAGATTQTAHNGQSVV
                     DNRLINPEIGIHITVDDVSKDYYVPMGTVSNALNYLNISLSDDDILNADLSDTVYLGQ
                     EIKIDRVNYACYPIYKSIPYKTVEQESDKLFVGQTKMLQKGKPQYRIHI"
     gene            complement(1163..1591)
                     /locus_tag="PLS64_08670"
     CDS             complement(1163..1591)
                     /locus_tag="PLS64_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43928.1"
                     /translation="MERERSRQEQTADTDKLYPQIAEALNISISQLEQYPSDMKQILC
                     QAYINNCELGKDDVAEALNGIVNLDVDEVGIKEVEKQQDNTAVDNELRQTEVTERKNK
                     RSYLLSRNQILQNARTIADSDKSKQYDRQQPENITRNNNS"
     assembly_gap    1758..1817
                     /estimated_length=60
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1818..>2475)
                     /locus_tag="PLS64_08675"
     CDS             complement(<1818..>2475)
                     /locus_tag="PLS64_08675"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRL43929.1"
                     /translation="RTFTCELKSNNSDKPFVSKATYPVNLGWKFIDISAKEKDKNEVD
                     KLCCGDEVPDKIQRNSKYCSVPASVSEHFTSPPKQYTDATLLSAMETAGNTDYVEDSN
                     VEKKGLGTPATRAGIIENLVKRQYIQRDGKKILPTEKGIQLIDCVPDDVKSPKMTAVW
                     ETALQHIEKGAASADEFMNQIEDSVKYLVDTYSEVDNSKHFTRWETVGICPKCGQKVL
                     S"
BASE COUNT          634 a          463 c          452 g          866 t
ORIGIN      
        1 aacagcgata acgcaaattc tttttttgtt tttcatttgc tgccatccct ttcattttta
       61 aggattctta aaccctttct gattatatta tacatcattt aggggtaaag tcaagtattt
      121 ttggaatttt ttcaaaaaaa gttttatttt ttctgccaac ggtcaaagaa gagtacattt
      181 tcatattgca agataaagct ttgtgttgaa tgaaaattac cgggtgtgct gtcataataa
      241 tcctttgtac acatataaaa cagcctgtgc ctgaccgcaa ggttgttatc atcgaatatg
      301 tatttgacag cattaactgc gttctgcgaa cggttggcag ttgagccgac atatcccata
      361 cctttaatta cagccgatac ggaagtatag ccgtcataca ctatggcaca cttgattgac
      421 tgagcaataa ggcaagagcc tacataactg ccgccaaatt cgcccgtaac aattcgttca
      481 agcatatccc tgtccttatc cgacagcttt accataggta atttaaagtc tttgttatca
      541 tattttgttt tgataagata gcttgttttg ttattataag atttattgat aatatcaata
      601 ttcctgttac ctttaactat aatcttatcg gtcggatatg tcaatacttg ttgcttaata
      661 catttatgtg atataagttc gccgtttaca tatttatcct tatatatgta ttctgtactg
      721 cggtttgcct ttttggagca ttttggtttg tccgacaaaa agtttgtccg actcctgttc
      781 aacagttttg tatgggatag atttgtatat cggatagcaa gcataattaa cacggtcaat
      841 tttaatctct tgaccgagat agaccgtgtc tgataaatca gcatttaata tatcatcatc
      901 cgagagtgat atattaagat aatttagtgc attgctaact gtccccatag gcacataata
      961 atctttgctt acatcatcta cggttatatg tattcctatt tcgggattta taagtctgtt
     1021 atcaacaaca gactgcccat tatgagcagt ctgtgttgtt gcacctgcta tgggtgctgt
     1081 attgagtaac gctgtcatag atattatgac agacaaaaag gagagcttaa ttgttgtatg
     1141 tataaatttg ttcataatcc acctaactgt tattattcct tgttatgttt tccggttgct
     1201 gacggtcata ttgttttgat ttatcggagt cggcaattgt tcgtgcattt tgaagaattt
     1261 gatttctgct caataagtat gaccgtttgt tttttcgttc tgtgacttcg gtttgccgta
     1321 actcattatc aacggcagta ttgtcctgtt gcttttcaac ctctttgata cccacctcat
     1381 caacatcaag attgactata ccgttcagag cttcggcaac atcatccttg ccaagctcac
     1441 aattgttgat gtaagcctga caaaggatct gtttcatatc ggatgggtac tgctcaagct
     1501 gagagataga tatattaaga gcttctgcta tttgtggata cagcttgtcg gtgtcggcag
     1561 tctgttcctg tcttgagcgt tctcgttcca tagtttactt cctgtaagat tttttagatt
     1621 ttgaagagct tgaaaaaaca aactctgttt taaaatccga gttaagttta atagctgcgt
     1681 caaaggattc gcctaatttg gacttcaagc ccttgagcgt gtccgtcttg cctttggtaa
     1741 ggagctttac aacctgtnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnatg acaatacttt ctgtccgcat ttagggcaaa tgccgactgt
     1861 ttcccaacgg gtaaaatgtt tactgttgtc aacttcgctg tatgtatcaa caagatactt
     1921 tacgctgtcc tcaatctgat tcataaattc atcggcactt gcagcacctt tttcaatgtg
     1981 ttgcaaagcg gtttcccata cagccgtcat tttcggagat ttgacatcat cgggaacaca
     2041 gtctatgagc tgaatgcctt tttcggtcgg cagaattttc ttaccatcac gctgtatgta
     2101 ttgtcgctta acaaggtttt cgataatgcc tgcacgggtt gcaggagtac cgagtccttt
     2161 tttttcaaca ttgctgtctt caacataatc ggtatttccg gctgtttcca tagcagacaa
     2221 taaagtagcg tccgtatatt gctttggcgg tgaagtgaag tgttctgata cgcttgcagg
     2281 tacggagcaa tatttactgt tacgctgtat tttatcaggc acttcatctc cgcaacacaa
     2341 cttatctact tcgtttttat ctttttcttt agcagatatg tcaataaatt tccaaccgag
     2401 attaacggga tatgttgctt tggaaacaaa tggtttatcg ctgttattag attttaattc
     2461 acaagtgaat gtacg
//
LOCUS       DAOMVE010000194         2472 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_12604_length_2472_cov_2.948697, whole genome shotgun sequence.
ACCESSION   DAOMVE010000194 DAOMVE010000000
VERSION     DAOMVE010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12604_length_2472_cov_2.948697"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..>2472)
                     /gene="uvrA"
                     /locus_tag="PLS64_08680"
     CDS             complement(86..>2472)
                     /gene="uvrA"
                     /locus_tag="PLS64_08680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRL43930.1"
                     /translation="TGTKIQVMAPVVRGRKGEHAKVLDSAGKSGYVRARVDGIIYDLS
                     ENIPIEKNKKHNIEIIVDRLVIKPEIASRLSDSIETASKLGGGLVTVNFNGDEDVTFS
                     QKYACPEHGVSIDELSPRMFSFNNPFGACPKCTGLGVFLKIDENKIIPDMSKSIRDGG
                     IKGSGWAMEGSTIAAMYMEALAKKYNFSLDEPLENLPKETIDKILYGTGDDELTINKK
                     SVYGGGTYQHKFEGIINNLERRYHDTSSNWIKDEIRSYMAEIPCDLCHGDRLSAESLA
                     VTVGGLNIADFCKMSVIDALEFVGQLKLTEKEQIIGAEIIKEINERLNFLKSVGLGYL
                     TLSRSSGTLSGGESQRIRLATQIGSSLMGVLYILDEPSIGLHQRDNEKLLATLKRLRD
                     LGNTVIVVEHDEDTMYAADCIVDVGPGAGIHGGEIVCVGDVEKIKQCPNSITGKYLSG
                     ERKVPVPEKRRKGNGLFLEVRGAAENNLKNINVKIPLGEFVCVTGVSGSGKSSLVNEI
                     LYKALARDLNRAKTIPGKHKEIRGMENLDKVIAIDQSPIGRTPRSNPATYTGVFTDIR
                     MLYAATTDAKMRGFTPSRFSFNVKGGRCEACQGDGIIKIEMHFLSDVYVPCEVCKGKR
                     YNRETLEVKYKGKSINDVLEMSVEEGMEFFSNIPKIYRKLKTLYEVGLGYIKLGQPAT
                     TLSGGEAQRVKLATELSKRSTGKTIYILDEPTTGLHIADVHRLVDVLQLLVDGGNTVV
                     VIEHNLDLIKTADYLIDLGPEGGDGGGTIVAKGTPEQVAKVEKSFTGQYLKPLL"
BASE COUNT          624 a          606 c          497 g          745 t
ORIGIN      
        1 atctttctat tccgtctgtc cgttctccat cccacaaaaa atcgtgatga accatttttt
       61 aaacaattgt ttttctctat aagaattata gcaacggttt aagatactgt cccgtaaatg
      121 atttttcaac ctttgcaacc tgttcgggag tgcctttggc aacaattgta ccgccgccgt
      181 caccgccctc gggaccgagg tcgataagat aatcggctgt cttgataagg tcaaggttgt
      241 gttcaattac aacaaccgta tttccgccgt caacaagaag ctgaagaaca tcaacaagtc
      301 tgtgtacatc ggcaatgtgc aagcccgttg tcggctcgtc aagaatataa attgtcttgc
      361 ccgtactgcg ttttgaaagc tctgttgcaa gctttacacg ctgagcctca ccgcccgaaa
      421 gcgttgttgc aggctgaccg agctttatat aaccaagacc cacttcatac agggttttca
      481 gctttctgta aatcttcggg atatttgaga agaactccat tccctcctca acggacattt
      541 caagaacatc attgattgac ttgcccttgt actttacttc gagggtttca cggttatatc
      601 tcttgccctt gcagacctca cacggaacat aaacatctga caaaaagtgc atttcaatct
      661 tgataatacc gtcaccctga caagcctcac atctgccgcc ctttacattg aatgaaaatc
      721 tgcttggtgt aaaaccacgc attttcgcat cggttgtagc ggcataaagc attctgatat
      781 ccgtaaacac acctgtgtat gttgcaggat ttgaacgggg agttctgccg ataggacttt
      841 ggtcaatggc aattacctta tcaagatttt ccatacccct gatttccttg tgcttgccgg
      901 ggattgtctt tgctcgatta agatcacggg caagggcttt gtaaagaatt tcgtttacaa
      961 gtgagctttt gcccgaaccc gaaacacctg ttacgcagac aaattcaccg agcggaattt
     1021 taacattgat atttttaagg ttattttcag ccgctcctct gacttcaagg aaaagtccgt
     1081 tgccttttct gcgcttttcg ggaacgggaa ctttgcgttc gcccgaaaga tacttgcctg
     1141 tgattgaatt tggacattgc ttaatctttt caacatcacc gacacagaca atttcaccgc
     1201 cgtgaatacc tgccccgggg cctacatcca caatgcaatc tgcggcatac atagtgtcct
     1261 catcgtgttc aacaacgata acagtatttc cgaggtcacg aagtcgcttt aaggttgcaa
     1321 gaagtttttc attgtcacgc tgatgaagtc cgatactcgg ctcgtcaaga atatacagca
     1381 cacccataag cgaactgccg atttgcgttg caaggcggat tcgctgactt tcaccgccgc
     1441 tgagtgttcc cgagcttctt gaaagagtaa ggtagccaag tcctacgctc ttgaggaagt
     1501 taagtctttc gttaatttct tttataattt cagcaccgat aatctgctct ttttctgtaa
     1561 gcttgagctg acctacaaat tcgagtgcgt caataaccga cattttgcag aaatcggcaa
     1621 tgttaagtcc gccgactgtt actgcaaggc tttcggctga aagtctgtca ccgtgacaaa
     1681 gatcgcacgg aatttctgcc atatacgaac ggatttcatc cttaatccag ttactgcttg
     1741 tgtcatgata acggcgttca aggttgttga ttataccctc aaatttatgc tgataagtac
     1801 ctccgccgta aaccgatttt ttattgattg tcagctcatc atcacctgtg ccgtagagga
     1861 ttttgtcgat agtttccttc gggagatttt caagcggttc atcaagcgaa aaattgtact
     1921 ttttcgctaa agcctccata tacattgcgg caattgtact gccttccatt gcccatccgc
     1981 tgcccttgat accgccgtca cggatactct ttgacatatc gggaataatt ttgttctcat
     2041 caattttgag gaatacgccg agtcccgtac actttgggca cgcaccgaac ggattgttga
     2101 atgaaaacat tctcggtgaa agctcgtcaa tgctcacacc gtgttcgggg caggcatatt
     2161 tttgtgaaaa agtcacatcc tcatcgccgt tgaagttcac cgtaacaaga ccgccgccaa
     2221 gctttgacgc tgtttcgatg ctgtctgaaa gtcttgatgc tatttcaggc ttgattacaa
     2281 gacggtcaac aatgatttct atattatgct ttttattttt ctcaatcgga atattttccg
     2341 acaaatcata gataatcccg tcaactcttg cacggacata gcccgatttt ccggcactgt
     2401 caagaacctt tgcgtgttcg ccctttctgc cccttacaac gggagccata acctgaattt
     2461 tcgtacccgt tt
//
LOCUS       DAOMVE010000195         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_13219_length_2379_cov_3.174699, whole genome shotgun sequence.
ACCESSION   DAOMVE010000195 DAOMVE010000000
VERSION     DAOMVE010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13219_length_2379_cov_3.174699"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1089)
                     /locus_tag="PLS64_08685"
     CDS             complement(94..1089)
                     /locus_tag="PLS64_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRL43931.1"
                     /translation="MKSKLFTHKFFVVLCISVAALLGLSFLARATIVNNRFEQMAVDE
                     KYSLKASADGQFLIQFQDDENGNFYATVGLFQGGYYGLDDAYIPTSYDENTKITAIEK
                     EAFSEFDCLKKVVIPNGICEIKDDAFKDSKNITEIYIAPSVNKIAKTAFDGIDNLTIY
                     AEKGSYAEKFAVENKINYKNYTTEPENPEAKNTDYSKIRNGAYYGEYYNYDVIYDDGK
                     PVCVITNYNPMSTEEKLEIPAHIDGLDVISIADDAINYGGAKETVVPDTVKFIADNAF
                     KESYSLEDIYLTKNVSYIGKSAFKDSKDLTIHAPTDSYAHTFATENKINFKATDD"
     gene            complement(1079..1702)
                     /locus_tag="PLS64_08690"
     CDS             complement(1079..1702)
                     /locus_tag="PLS64_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43932.1"
                     /translation="MVEIKNYCKSIKSRPILNNVSYNFEYGKIYGIYGHNGSGKTMLL
                     RAIAGLLVPDSGSVVIDGKVLHKDMSFPPSIGIVIENMNLLPQYNAFDNLKILGKIKK
                     TATDEDIKTALERVGLKSDLKVKKFSLGMKQRLNIAQAVFEKQKIILLDEPTNALDND
                     GVQLIYKLLKEEKERGALVVITTHHKEDLEEVCDVVLEMTEGELHEK"
     gene            complement(1712..>2379)
                     /locus_tag="PLS64_08695"
     CDS             complement(1712..>2379)
                     /locus_tag="PLS64_08695"
                     /inference="COORDINATES: protein motif:HMM:NF022373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2705 family protein"
                     /protein_id="HRL43933.1"
                     /translation="IILIVACFAQLFSCVFQGDFQNAFMIGLAPSDYGLEITKFLPLL
                     LPVCFILFFTSGSIENLKQGYGKMLIVRNYSKTVLILKRCLNNFIALICIVLFQFIIF
                     FVARESMTPVESGTLKSLIMYFLTIFSLIVIQSLLEISIPAHIVNIGIFTYCFIAYYL
                     VQNFVDAPIWKMILFPSLMFGMQNGAVSGESIYYGYLLFIVALNALCIFILNLRFKKT
                     DIF"
BASE COUNT          728 a          485 c          420 g          746 t
ORIGIN      
        1 cactgtccca ttatctaccc tttgattgca aaaacagccc tgcaaatggg tacagggctg
       61 taaatttaaa gatttattca ttgcttatca gatttaatcg tctgtagctt tgaaatttat
      121 tttattttcg gtcgcaaagg tgtgtgcata ggaatctgtc ggggcgtgga ttgttaggtc
      181 ttttgagtct ttgaaggcag attttcctat gtaggacaca tttttggtca ggtagatatc
      241 ctcaagggag taggattctt tgaacgcatt atcggcaatg aattttactg tgtcgggaac
      301 gactgtttcc tttgcaccgc cataatttat tgcgtcatct gcaattgaaa tcacatccaa
      361 gccgtcaata tgtgcaggaa tttcaagctt ttcctctgtc gacattgggt tatagttagt
      421 tattacgcac acgggcttgc cgtcatcata gataacatca taattataat attcgccgta
      481 atacgcaccg tttctgattt ttgaataatc ggtattttta gcctccggat tttcaggctc
      541 ggtcgtatag tttttataat tgattttatt ttcaaccgca aatttttcag cataagaacc
      601 cttttcggca tatatcgtaa ggttgtcaat gccgtcaaaa gcggtttttg caatcttatt
      661 tacggagggc gcaatataaa tttccgttat attttttgaa tccttaaatg cgtcatcctt
      721 gatttcacaa atgccgttgg ggataacaac ttttttcaga caatcaaatt cgctgaaagc
      781 ttccttttca atagctgtta tctttgtatt ttcatcatat gaagtaggaa tatacgcatc
      841 gtccaagccg taatatccgc cctgaaacaa gccgacagtg gcataaaaat ttccgttttc
      901 atcgtcttga aactgaatta aaaactgacc gtcggcagaa gcctttagac tatatttttc
      961 atcaacagcc atttgttcaa atctgttatt tacgattgtc gctctggcaa ggaaagacaa
     1021 tccgagcaaa gcggcaaccg agatgcaaag aacaacgaaa aatttatgag taaacagttt
     1081 acttttcatg cagctcacct tctgtcattt ccaaaacaac atcacaaact tcttccaaat
     1141 cctctttgtg atgagttgtg ataactacga gcgcacctct ttctttttct tctttaagaa
     1201 gcttgtaaat cagctgtaca ccgtcattat caagtgcatt tgtcggttca tcaagcagga
     1261 ttattttctg cttttcaaac acagcctgag caatattcag cctctgtttc ataccaagcg
     1321 aaaacttttt cacctttaaa tcggacttta atccaacacg ctcaagcgct gttttaatat
     1381 cttcatcggt ggcggttttc ttgattttgc cgaggatttt cagattgtca aaagcattgt
     1441 actggggcaa caggttcata ttttcaatca cgatgcctat gctcggaggg aacgacatat
     1501 ccttgtgcaa aacctttccg tcaatcacaa cagagccgct gtcgggaaca agcaagcctg
     1561 caattgctct caaaagcata gttttgcccg aaccgttgtg tccgtaaatt ccgtagattt
     1621 tgccgtactc aaaattgtaa cttacattat ttaaaatcgg tcttgatttg atacttttac
     1681 aataattttt tatttctacc ataatatcac ctcaaaaaat atcggttttc ttaaatctca
     1741 aattcagaat gaaaatgcac aaagcattta acgcaactat gaataacagg tatccgtagt
     1801 aaatgctctc gcccgacaca gcaccgttct gcattccgaa catcaaactc gggaagagaa
     1861 tcattttcca aatcggtgcg tcaacgaaat tctgaaccaa atagtacgca ataaagcaat
     1921 aggtgaaaat tcctatgttc acaatgtgtg caggaatact gatttcaagc aaagactgaa
     1981 taactatcag agaaaaaatt gtcaaaaagt acataatcaa agatttaaga gttccgcttt
     2041 caacgggagt catactttct cttgcaacaa aaaagattat gaactggaac aaaacaatgc
     2101 aaatcaatgc tataaaattg ttaagacaac gctttaaaat cagcactgtt tttgaataat
     2161 tgcgtacaat caacatcttg ccgtagcctt gtttaaggtt ctcaattgag ccgcttgtaa
     2221 agaagagaat aaaacacaca ggcagcaaaa gcggcagaaa ttttgtaatt tcaaggccgt
     2281 aatcgcttgg ggctaaacca atcataaagg cattttgaaa atctccctga aatacacaac
     2341 tgaacaattg agcaaagcac gcaacaatca gtattatcg
//
LOCUS       DAOMVE010000196         2356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_13376_length_2356_cov_3.946545, whole genome shotgun sequence.
ACCESSION   DAOMVE010000196 DAOMVE010000000
VERSION     DAOMVE010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13376_length_2356_cov_3.946545"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..2206
                     /locus_tag="PLS64_08700"
     CDS             317..2206
                     /locus_tag="PLS64_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRL43934.1"
                     /translation="MGSDYMPVPFDSRKIYYRSPFGAVEEGTEIHFRILLPKAEHTQK
                     AHLCVKYDYDCNWEFTELIWCGKFDEDTEIWECDFTPKKIGLYWYNFKLTAIDKTRYV
                     IPSDPYKVSTIEDYMGRSWQITCYKKGFKTPGWLVGGIMYQIFPDRFYFSGEEKNIKR
                     TDFKRNKDWYALPEWKPDENGMIKNNDFFMGDLKGITMKLDYLESLGVSCIYLNPISE
                     AYSNHRYDTGDYSKVDPILGTEEDFKHLCAEAKKRGIHIINDGVFSHTGSDSVYFNRY
                     GKYGKGGAYNDKNSPYFSWYKFNEWPNNYQSWWGFDTLPEVIEETPSFNEYINGKDGI
                     VRKWMKCGNSGWRLDVADELPDVFIENLRKAVKDENPNAFLVGEVWEDASNKESYGAR
                     RKFLLGEQFDSVMNYVFRDAILNFCKGQHGKYTMDLIMSVIENYPRPVLRVLMNSLST
                     HDTERAITVMAGEPLDGKDREWQADTKLDDEHKKLGVKLLKVASAMQFTLPGFPCIYY
                     GDEAGMEGYRDPFNRCCYPWGKENKELIEWHKKLADVRKSCSALWDGDFINVLSEERR
                     ISYIRHDKDSAIFCTFNLYDYEVEAKMPPGYADGKPVFGSKLENGILTIPPMSAEFVV
                     LELGK"
BASE COUNT          718 a          453 c          518 g          667 t
ORIGIN      
        1 tactctcttt ttctccatag gaaatgcatc tcccttttat agctttttcg tcctcatttg
       61 acttctttgc atacataatc attttacatt ataatataaa attggaaata ataaaagagc
      121 aaagagaata ttgtttaaat attttttcat tcgtagacaa atttggcgat tttatgctgt
      181 ttacaactta tattggttga aatatttatt ctcttatgat aaaataatct ttgcgtcatt
      241 ttggcggatt tgtcttaatt tccgattaac agggcataac cgccaaaaca aaattgacat
      301 ttcggaatat attattatgg gaagtgatta tatgcccgta ccgtttgatt cacgaaaaat
      361 ttactatcgt tcaccgtttg gtgcggttga agagggtacc gagattcatt tcagaattct
      421 tttgcctaag gcggagcata cacaaaaagc acacttgtgt gtaaaatatg attatgactg
      481 caattgggaa tttaccgagc ttatctggtg cggtaaattt gacgaggata ccgaaatttg
      541 ggagtgcgat tttaccccga aaaaaatcgg actttattgg tataacttta agcttactgc
      601 cattgataaa acaagatatg ttattccgtc cgatccgtat aaggtcagca ccattgaaga
      661 ctatatgggc agaagctggc agattacctg ctacaaaaaa ggctttaaaa ctcccggctg
      721 gcttgtcggc ggaattatgt atcagatttt tcccgaccgt ttttatttca gcggtgaaga
      781 aaagaatata aagcgtaccg acttcaaacg caacaaggat tggtatgctc tccccgaatg
      841 gaaacccgat gaaaacggaa tgattaagaa caatgatttc tttatgggcg acctcaaggg
      901 aattacgatg aagcttgatt accttgaaag cctcggtgta agctgtattt accttaatcc
      961 tatcagcgag gcatactcaa accaccgcta tgatacgggc gactactcaa aggttgatcc
     1021 gattctcgga acggaggaag attttaagca cctttgcgca gaggctaaaa agaggggaat
     1081 acacatcata aatgacggtg tgttctctca tacgggaagt gacagcgttt acttcaaccg
     1141 ttacggcaaa tacggcaagg gcggtgcgta taatgacaaa aactcaccgt atttcagttg
     1201 gtacaaattc aacgaatggc cgaacaatta ccaaagctgg tggggctttg atactctgcc
     1261 cgaggttatt gaggaaactc cgtcattcaa cgaatatatc aacggcaaag acggcattgt
     1321 cagaaaatgg atgaaatgcg gcaactcggg ttggcggctt gatgttgcag atgaacttcc
     1381 cgatgtgttc attgaaaatc tacgcaaagc cgttaaggac gaaaatccga acgcctttct
     1441 tgtaggtgaa gtgtgggagg acgcaagcaa caaggaaagc tacggtgcaa gaagaaagtt
     1501 cttgctcggc gaacagtttg actccgttat gaactatgtg ttcagggatg caattctcaa
     1561 cttctgcaaa ggtcagcacg gcaaatacac aatggatctg attatgagcg taatcgaaaa
     1621 ttatccccgt cctgttctca gagtgctgat gaattcactt tcgactcacg ataccgaaag
     1681 agcaatcaca gttatggcag gcgaaccgct tgacggcaag gacagagaat ggcaggctga
     1741 cactaagctt gatgacgagc ataaaaaact cggtgtaaag ctgctcaagg ttgcaagtgc
     1801 aatgcagttt actctccccg gatttccgtg tatttattac ggtgacgagg caggaatgga
     1861 gggatatcgt gatccgttca accgttgctg ttatccgtgg ggcaaagaaa ataaggaact
     1921 cattgaatgg cacaaaaagc ttgccgatgt tagaaaaagc tgttcggcat tgtgggacgg
     1981 cgattttata aatgttcttt cggaggaaag aagaatttca tacatccgcc acgacaaaga
     2041 ttccgctatt ttctgcacat tcaatctcta tgattatgaa gtcgaggcaa aaatgccgcc
     2101 cggatatgca gacggcaaac ctgttttcgg ttcaaagctt gagaacggta tactcacaat
     2161 tccgcccatg tccgcagaat ttgtagttct tgaactcggt aaatgatgta tatagtataa
     2221 aacagaataa aatataatta caagcgacag aaattaaact gtcgcttgtt ttgttgcggt
     2281 atttatatgt ttgttatatt taatttaccg acttactcgc cttccgttat cggcggaata
     2341 aagcattcta tcgcac
//
LOCUS       DAOMVE010000197         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_13453_length_2346_cov_3.280663, whole genome shotgun sequence.
ACCESSION   DAOMVE010000197 DAOMVE010000000
VERSION     DAOMVE010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13453_length_2346_cov_3.280663"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLS64_08705"
     CDS             complement(<1..367)
                     /locus_tag="PLS64_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRL43935.1"
                     /translation="MAVKIFDVTTGSHADKAGIKKGETLLSINSNEIVDVLDYRFYQV
                     NRKLTLEVADENKNVRTIEMTKGEYEEIGLEFETYLMDKQHSCRNKCIFCFIDQLPKG
                     MRESLYFKDDDSRLSFLFGN"
     gene            complement(524..712)
                     /gene="rpmF"
                     /locus_tag="PLS64_08710"
     CDS             complement(524..712)
                     /gene="rpmF"
                     /locus_tag="PLS64_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRL43936.1"
                     /translation="MAVPKNKTSHARKSSRRSTVWKINAPELTACPNCGELTAAHKAC
                     TNCGMYNGRQVVVKKDEQ"
     gene            complement(740..1234)
                     /locus_tag="PLS64_08715"
     CDS             complement(740..1234)
                     /locus_tag="PLS64_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRL43937.1"
                     /translation="MLFELKSVFQNDGEEKQVEYNLDLSGLDVDGVYPFKTPVVVTAK
                     ATNRASLITLVINAHFDYSRDCDRCGEPYTREMNMSFEHRLIQTLADEENDDYIETPD
                     FTLELDDVVISDIFLSLPSKNLCRDDCKGLCQICGQNLNNGECSCDKRQTDPRLEILK
                     QLID"
     gene            1656..>2346
                     /locus_tag="PLS64_08720"
     CDS             1656..>2346
                     /locus_tag="PLS64_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRL43938.1"
                     /translation="MERYSIINEKNNREIVLLRGSGCVYRKCIFCDYYTDRCSDEDEN
                     FRLNKAVLEKVTGIFGDLEVINSGSVFELDKKTLDFIKQVCKEKKINTIHFEAHYLYR
                     NKISQLRKDFADFDLKMKLGLETFDCDFRENVLKKGIKESSPAVIAENFDEANFLFGI
                     KGQTAETMQKDIELGLKYFERICVNIMCDNTTEVEPDKAVIKEFMQKIYPVYKDNPRT
                     DILINNTDFGVG"
BASE COUNT          723 a          459 c          455 g          709 t
ORIGIN      
        1 agttgccgaa caaaaacgaa agtcggctgt catcgtcctt aaaatagagc gactcacgca
       61 tacccttcgg aagctggtca ataaagcaga aaatgcactt attacggcac gaatgctgtt
      121 tgtccataag ataggtttca aactcaagac cgatttcctc atattcgccc ttggtcattt
      181 ctatggtacg aacatttttg ttctcatccg caacctcaag agtcagcttg cggtttacct
      241 gatagaatcg gtaatcaaga acatcgacaa tctcgtttga attgattgaa aggagggttt
      301 cccctttttt tatccctgct ttgtcggcat gactgccggt tgtgacatca aaaattttaa
      361 cagccacacc ttcacctcgc aaaacatttt tacatacact tttattctac acaaaatacc
      421 gtttttaatc aacaggtttg caaattgttt ttggatatac ggtaaaattt gcaaaacaaa
      481 agggcgaaca gataactgcc gcccgtaatg tttgtaataa aaattactgt tcgtctttct
      541 ttacaacaac ctgtctgccg ttgtacatac cacagtttgt gcaagcctta tgagctgctg
      601 ttaattcacc gcagttagga caagcagtaa gttcaggagc attaatcttc catactgttg
      661 aacgtcttga gctctttctt gcgtgtgatg ttttgttctt gggtactgcc attacaaaaa
      721 cctccttaca aaatttatct tagtcaatca actgtttaag tatctcaagt cttggatccg
      781 tttgacgctt atcacaggaa cattcaccgt tgttgaggtt ttgtccgcaa atctgacaca
      841 gacctttgca atcgtcccta caaagattct ttgacgggag cgaaagaaaa atgtcagaaa
      901 ttacaacatc atcaagctcg agtgtaaaat caggtgtttc aatatagtca tcattttcct
      961 catcggcaag agtctgaata agcctgtgtt caaagctcat attcatctct cttgtataag
     1021 gctcaccgca acggtcacag tcacggctgt aatcaaagtg tgcattgatt acaagagtaa
     1081 tcaaacttgc tctgtttgtt gctttggcag tcacgacaac gggagttttg aatggataaa
     1141 ctccgtcaac atcaagacct gacaagtcaa ggttatattc aacttgtttt tcttcaccgt
     1201 cattctgaaa gacggattta agctcaaaaa gcatatctgc acctcttgac agcatagaca
     1261 catattaaaa cgctgtttat tattatacaa aaggtttgtc cctttgtcaa ctaaaaaata
     1321 caagaaaata ggctgttttt ttcgggaatt tatgacaata ttcgctaatt ttcccatatg
     1381 gaattttgag cctgcaaagt cactaagaaa gttactttgc acttgatttt cgcttttcat
     1441 tatacctctt aaacatatat aatgcaataa taaattgcag ttgacataaa acggaatttg
     1501 tgatagcata ctgtgtggca gttcgtaacc atcctgccgg aaggacgccg ataggctgct
     1561 ccttctatca aaactaagga gaaaggcaca gggctttttg cgcccttttt gcaccctctt
     1621 ttgtgtgggt gctttttttg tgccggaaag agagtatgga gagatacagt attattaatg
     1681 aaaagaacaa tcgggagatt gttcttctgc gtggaagcgg ttgcgtttac agaaaatgca
     1741 tcttctgcga ctactacacc gacaggtgca gtgacgagga cgaaaacttt cgtcttaaca
     1801 aagccgtgct tgaaaaagtt acgggaattt tcggtgacct tgaggtcatt aactcaggct
     1861 cggtttttga gcttgacaaa aaaacacttg attttatcaa acaggtgtgc aaagaaaaaa
     1921 agataaatac cattcatttt gaggcacatt atctttatcg gaacaaaatc agtcagctca
     1981 gaaaagattt tgcggatttt gatttaaaaa tgaagctcgg tcttgaaacc tttgactgcg
     2041 atttcagaga aaatgttctg aaaaaaggaa tcaaagagag cagtcccgct gttattgcag
     2101 agaatttcga cgaagctaat ttcctttttg gaattaaagg tcagacagcc gaaaccatgc
     2161 aaaaggatat tgagttggga ttaaaatatt ttgaacgaat ttgcgttaac attatgtgtg
     2221 acaacacaac ggaagttgaa cccgacaaag ctgtcataaa agagtttatg cagaaaatct
     2281 atcctgtata taaggataac ccaagaacgg atattttaat taacaacacg gattttggag
     2341 taggtg
//
LOCUS       DAOMVE010000198         2265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_14057_length_2265_cov_3.350679, whole genome shotgun sequence.
ACCESSION   DAOMVE010000198 DAOMVE010000000
VERSION     DAOMVE010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14057_length_2265_cov_3.350679"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(396..1184)
                     /locus_tag="PLS64_08725"
     CDS             complement(396..1184)
                     /locus_tag="PLS64_08725"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRL43939.1"
                     /translation="MIKKILTGVAIAMLVVGIGLVAFPPISNFIGTQISNGEADGFDT
                     RVEEVEDGSFEQAVKDGKVDEDGYKIDREGKRTSKQPVVFKADLDRLFNDSVAYNKKL
                     ETNQYEMLRNDTYSQAAVNLEDYGITDGIYGYVTAETINMRLPIYLGANSSNMSYGAA
                     HMSATSLPIGGKNTNAVLAGHTGYIGRIFFDNLRNLKIGDRVSITNYWDTLNYKVVET
                     KVNKPSESGDCYLQKDRDLLTMFTCISDGNGGFDRYYVICERAS"
     gene            complement(1177..2121)
                     /locus_tag="PLS64_08730"
     CDS             complement(1177..2121)
                     /locus_tag="PLS64_08730"
                     /inference="COORDINATES: protein motif:HMM:NF033745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HRL43940.1"
                     /translation="MKRKLSILLIVLIFVAGIGIMSYPLVSSAINNYVSRSRVKEYTD
                     RVAQMPSEKTQKMFEEAAKYNNSLSNNMIITDPFDEKAFQKIGANYEKTLNIDDNGLI
                     GYIDVPKINVYLPIYHGTSEEILSKGAGHLQNTSLPVGGASTHSVISAHSGFPGETFF
                     DYLTDMKVGDEFYVHILDRTLKYEVDQIEVVLPSEINSLRIVDGEDLVTLLTCTPYGI
                     NTHRLLVRGKRVDYDDTKYIETGESLAKFDNGYIFFLGYKIPYAVAIGIIVGFVALVV
                     FVVVFLLKRKKKKSNGAKRLRDDLADENQNDLGGDSDD"
BASE COUNT          612 a          497 c          446 g          710 t
ORIGIN      
        1 acaaaaagct gattgtttga gattttctgc gttgtacctg taacttcaaa gtccttgtga
       61 ttttcatcct cgcttaccgc acctgtcata agaaccgaac cgacggtaag cacaccgcac
      121 attgcgattg cagttatttt tttaattata tttttcattg ttatcctccg tgtttgtaat
      181 tttatccgca aatttgttca tacttgtcta agtttatcaa tctatttttc ttatgtcaat
      241 aattttaaaa taaattcggc atttacggca atatatgcgt atttttcaaa tattaactat
      301 tacaaatgac agacaaaata ctcggtcatt tctgttttaa acaattggtc tttaaacaaa
      361 aaaacagact gcaccgtgat gcagtctgct gtaaattatg acgcacgctc acatattaca
      421 tagtaacggt caaaaccgcc gttgccgtca cttatgcagg taaacatcgt gagcaaatct
      481 ctgtccttct gcaaatagca gtcgcctgat tcgctcggct tattgacttt ggtttcaacg
      541 accttgtaat tgagtgtatc ccaataattt gtgattgaaa ccctgtcacc gattttgagg
      601 ttgcggaggt tgtcaaagaa tattcgaccg atgtatcccg tgtgacctgc aaggactgcg
      661 tttgtattct taccgccgat cggcaatgag gttgcactca tatgtgccgc accgtagctc
      721 atatttgagc tgttcgcacc gaggtaaatc ggcagacgca tatttattgt ttcggcggta
      781 acatagccgt aaatgccgtc ggttatgccg taatcctcaa gattgacggc agcctgtgaa
      841 taggtatcgt tgcggagcat ttcatactgg tttgtttcaa gttttttgtt gtaggcaacg
      901 ctgtcattga aaagtctgtc gaggtcagcc ttgaaaacaa cgggctgttt ggaagtacgc
      961 ttgccttccc tgtcaatttt gtaaccatct tcgtcaacct tgccgtcctt gaccgcctgt
     1021 tcaaaagagc cgtcctcaac ttcttcaaca cgggtatcaa agccgtcagc ctcaccgttg
     1081 gaaatctggg ttccgataaa atttgatata ggggggaatg cgacaagacc tattccgaca
     1141 acgagcattg ctattgccac acccgtaagt atttttttaa tcatcggaat caccgcccaa
     1201 atcattctga ttttcgtcag ccaagtcatc tctcagccgt tttgcaccgt ttgacttctt
     1261 ctttttacgc ttgagcagaa atacaaccac aaacacaaca agtgcaacaa agccgacaat
     1321 tatgccgatt gcgactgcat aagggatttt gtaaccgaga aagaatatgt aaccgttgtc
     1381 aaattttgca agcgactcgc ccgtttcaat atactttgta tcgtcataat cgacacgctt
     1441 gccacgcacg agaagtctgt gagtattaat gccgtatggg gtacaggtga ggagcgttac
     1501 aaggtcttca ccgtcaacaa ttcgcaagga attgatttcc gagggaagaa caacttcgat
     1561 ttgatcaacc tcatatttca gcgttctgtc gagaatatga acataaaatt catcgcctac
     1621 tttcatatca gtgagataat caaagaaagt ttctcccgga aaacccgaat gggcagaaat
     1681 tacggaatgt gtgcttgcac cgccgacagg cagggaagtg ttttgaagat gccctgcacc
     1741 tttagaaaga atctcttcgc ttgtgccgtg ataaatcggc agataaacat tgattttcgg
     1801 cacatcaata tagccgataa gaccgttatc atcaatattg agagttttct cataatttgc
     1861 accgattttc tgaaaagcct tttcgtcgaa cgggtcggta attatcatat tatttgaaag
     1921 gctgttattg tacttagcag cctcttcaaa cattttttga gttttctcac tcggcatctg
     1981 agcaaccctg tcggtgtact ccttaaccct gcttcgactg acatagttat taattgcgga
     2041 actgacaagc ggatagctca ttatgccgat tcctgcaacg aatattaaaa ctattagtaa
     2101 aattgacagc tttcgtttca taaaattttc cttttctgcg tgtttttgtt ccgttgttat
     2161 tatactatat tttactattt atcgcaactg cgtgccgcag tcccagttcg tgaagatagt
     2221 ttgtgcaatg ccaaaacata ttccgcccga gcgtagcatt gtaat
//
LOCUS       DAOMVE010000199         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_14177_length_2250_cov_3.135308, whole genome shotgun sequence.
ACCESSION   DAOMVE010000199 DAOMVE010000000
VERSION     DAOMVE010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14177_length_2250_cov_3.135308"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..684)
                     /locus_tag="PLS64_08735"
     CDS             complement(37..684)
                     /locus_tag="PLS64_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRL43941.1"
                     /translation="MFKGAVFDMDGLMIDSERLVYSIWQEMLDEDGKEFNLDIFKKTI
                     GLRSEDSYEYYKSVYGDDFDYPAYKAESRIRYFKRIEKNGVPVKKGLYEIFDYLKSVG
                     CKISLATSTSSQTALKIMKKINLYDKFDAFVCGDDVKNGKPDPEVFLIAAKKLGLEPV
                     DCVAFEDSINGIKSAHAAGMTTVMVPDLSEPTDEIKPMIDFLCTDLSEAIERISN"
     gene            830..2194
                     /locus_tag="PLS64_08740"
     CDS             830..2194
                     /locus_tag="PLS64_08740"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRL43942.1"
                     /translation="MATSIYAKPIKAKTILKFTLPTVLMMVFMSLYTVVDGIVVANCV
                     GSDALSAINIVYPFTLVFMAVGLMFSTGSNAIIAKKMGEGKQEEANRLMSGVAITATV
                     ISVIIAVLFTIFAEPMYMMFGSNEKILPYCIDYGSVMIPYGFVMCWQMLNQSYLVTAD
                     KPHIMLVFSILSGCTNIVLDILFMAVWDFGIIGAGLGTIIGMAVGAIPLLLFFNKKQT
                     LHFGKPEFKVSEILFAMANGSSEAVTNLSTAVTTALFNIQMMHFAGAKGVAAISAILY
                     IHFIFTAVSFGFTSGVSPVISFNYGAQNHEKLQKLFKLCIKITLIISVAMITASEFFA
                     EPLVGIFSAGNEKIQQIALSGFRIFAINYLLCGMNIFASGLFTALNNGKISAVISVAR
                     TLVFECAAMLILPMFIGINGVWSALPVAELCAVAISVTFIIANAKKYHLVGVPPLPVR
                     ADSL"
BASE COUNT          633 a          487 c          446 g          684 t
ORIGIN      
        1 acacgacatt aattttccat tttcaatttt caattttcaa tttgaaattc gttctatcgc
       61 ttcgcttaaa tccgtacaca aaaaatcaat catcggttta atttcgtctg tcggctctga
      121 caaatcgggt accataaccg tggtcatacc tgcggcatgt gcagatttta tgccgttaat
      181 gctgtcctca aacgcaacgc agtccaccgg ctcaagcccg agcttctttg cggcaatcaa
      241 aaaaacctcg ggatcgggtt tgccgttttt aacatcatca ccgcacacga atgcgtcaaa
      301 tttatcatag agattgattt tcttcataat cttcaaagcc gtttgtgacg aggttgaagt
      361 tgcaagcgaa attttacaac cgactgattt taaataatca aaaatttcgt acaagccttt
      421 ttttacaggc acaccgttct tttcgatacg cttaaaatag cggattctcg actccgcctt
      481 gtaagcagga tagtcaaaat catcgccgta aaccgacttg taatattcgt aggaatcctc
      541 actgcgaagt ccgattgtct ttttgaaaat gtcaagatta aactctttgc cgtcctcatc
      601 aagcatttcc tgccagattg aataaacaag cctttcgctg tcaatcataa gcccgtccat
      661 atcaaaaact gcgcccttaa acataaaacc gccccttttt tcgcttttaa gtctgtatat
      721 aatagcatat tttacaaaaa aagacaaatc ggttgtaatt gcgacaaagt tatgatacaa
      781 tcgtttgaat ggtctgtgaa aaatataaat ttgagggtaa ggaagtttta tggcaacatc
      841 aatttatgca aaacctatca aagcaaaaac gattctgaag ttcactctgc caaccgttct
      901 tatgatggtt tttatgtcac tttacacggt tgttgacgga atcgttgttg cgaactgtgt
      961 cggaagtgac gcattgtccg caatcaatat tgtatatccg ttcacgctcg tatttatggc
     1021 tgtgggctta atgttttcaa caggctcaaa tgctataatc gcaaagaaaa tgggagaagg
     1081 caaacaggag gaagcaaacc gacttatgtc cggtgttgca atcacggcaa cggtcatttc
     1141 tgttatcatt gctgtgctgt tcacaatttt tgccgaacct atgtacatga tgttcggttc
     1201 aaacgaaaaa attctcccct actgcattga ctacggcagc gttatgattc cgtacggctt
     1261 tgtaatgtgc tggcagatgc tcaaccaatc gtaccttgta acagcagaca aaccacatat
     1321 catgcttgtt ttctcaatac tgtcgggttg cacaaacatt gtgcttgaca ttctttttat
     1381 ggcagtgtgg gattttggaa taatcggtgc aggactcggc acaatcatcg gaatggctgt
     1441 gggtgcaatt ccgcttttgc tctttttcaa caaaaagcag actctgcact ttggcaagcc
     1501 tgaattcaag gtcagtgaaa tcctctttgc aatggcaaac ggctcgtcag aggctgtgac
     1561 aaatctttca acagccgtga caacagcgct gttcaacatt cagatgatgc actttgcagg
     1621 cgcaaaaggc gttgcggcta taagtgctat tttgtacatt cactttattt tcactgcggt
     1681 ttcgtttgga ttcacttcgg gagtttcacc cgtcatcagc tttaactacg gcgcacaaaa
     1741 tcacgaaaaa ctgcaaaagc tgttcaaatt atgcataaaa atcacgctga taatttcggt
     1801 tgcaatgatt acggcatctg aattttttgc agaaccgctt gtcggaattt tctcggcagg
     1861 caacgaaaaa attcagcaga ttgcactcag cggattcaga atttttgcaa taaattatct
     1921 gttgtgcgga atgaatattt tcgcatcggg acttttcacc gcactcaaca acggcaagat
     1981 ttcagccgta atttcagttg caagaacgct tgttttcgag tgtgccgcaa tgctgatttt
     2041 gccgatgttt ataggcataa acggtgtatg gtcggcattg cccgttgcag aattgtgtgc
     2101 cgttgcaatt tccgtaacat ttataattgc caatgcaaaa aaataccatc tggtcggtgt
     2161 accaccgctc cccgttcgtg cagacagttt gtaatattga aagcatatta tgcccgagtg
     2221 tagcattgta accatttgta caaataaacg
//
LOCUS       DAOMVE010000200         2240 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_14262_length_2240_cov_2.408696, whole genome shotgun sequence.
ACCESSION   DAOMVE010000200 DAOMVE010000000
VERSION     DAOMVE010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14262_length_2240_cov_2.408696"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1165
                     /locus_tag="PLS64_08745"
     CDS             <1..>1165
                     /locus_tag="PLS64_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43943.1"
                     /translation="SYIDKDVSSGRNYTYTVRCITSDAKKFTSGYNPTGKSVKYVATP
                     KISKLEVTYDGVKLTWNKVAGAEKYRVYYKDRNGWTKLADTTGTTMLDMNVVSKEFDL
                     SIDDIYYTVRCISKDAKSFTSGYDSKGTPIGNHQDCPEIYSIGAVNNGVEMHWTGDAP
                     KFRVYIKENGSWKKIAETYDNYYVHKGAKSGSNTYTVRGVSKDGKKFTTMFNPTGVTY
                     RYKTSDKTRDAYINYMMNHQSEWMPSLGSDHNLSGVMFLDFDFDGVPELVAQKADSNE
                     YSFHSVVYKFEDGKLKRVGNVNDGFAIYYNKTAKKYEVHDLKISLNVDDGYYWSGNSI
                     ITYDGENLFANMYSARGVGEESNNNIYYNYFDGNDKNISRAKYNEINLKQFNN"
     assembly_gap    1166..1184
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1611..>2240)
                     /gene="ilvC"
                     /locus_tag="PLS64_08750"
     CDS             complement(1611..>2240)
                     /gene="ilvC"
                     /locus_tag="PLS64_08750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRL43944.1"
                     /translation="PKGPGHTVRSEYQVGKGVPCLVAVEQDATGQAKDLALAYALGIG
                     GARAGVLETTFRTETETDLFGEQAVLCGGVCALMQAGFETLCDAGYDPRNAYFECIHE
                     MKLIVDLIYQSGFAGMRYSISNTAEYGDYITGPKIITEDTKKAMRKVLSDIQDGTFAK
                     DFLLDMSDAGSQVHFKAMRKLAAEAPAEVFGKEVRKLYSWSDEDKLINN"
BASE COUNT          707 a          418 c          481 g          615 t
ORIGIN      
        1 cctcatatat cgacaaggat gtttcatccg gcagaaatta cacttatacg gtaagatgta
       61 ttacatcaga cgcaaagaaa tttacaagcg gttataaccc gacaggcaag tcggtaaagt
      121 atgttgccac accgaaaatt tcaaaacttg aggttacata tgacggtgta aagctgacat
      181 ggaacaaggt tgcaggtgca gagaagtatc gtgtgtacta caaggacaga aacggttgga
      241 caaagcttgc cgatacaaca ggcacgacta tgcttgatat gaatgttgtt tcaaaggaat
      301 ttgatttaag cattgatgat atttattaca cggttcgttg tatcagcaag gatgccaaga
      361 gttttacaag cggctacgat tcaaagggta cgcctattgg caatcatcag gattgtcccg
      421 aaatttacag cattggtgca gtaaacaacg gtgttgaaat gcattggacg ggagatgcgc
      481 caaagttccg tgtttatatt aaagaaaacg gttcttggaa gaagatagca gaaacttatg
      541 acaactatta tgttcacaag ggtgcaaagt ccggcagtaa cacttatacg gtaagaggtg
      601 tttcaaaaga cggtaaaaag tttactacga tgtttaaccc aaccggtgta acatacagat
      661 ataagacatc cgataaaaca agggacgctt atatcaatta tatgatgaat catcagagtg
      721 aatggatgcc aagcttggga agtgatcaca atttgtcagg tgttatgttt ttggatttcg
      781 actttgacgg agttccggag cttgttgcac aaaaggctga ttccaatgag tatagtttcc
      841 attccgttgt ttacaaattt gaagacggta agttaaaaag agtgggaaat gttaacgacg
      901 gttttgcgat atactataat aaaactgcca aaaagtatga ggttcacgac ctaaagatat
      961 ctttaaatgt agatgacggt tactattggt cgggtaattc aataatcaca tacgacggag
     1021 agaatctttt tgcaaatatg tattcggcaa gaggtgtagg cgaagaatca aataataata
     1081 tttattataa ttattttgac ggtaatgaca aaaatatttc acgggcaaaa tataacgaaa
     1141 ttaaccttaa acagtttaat aattgnnnnn nnnnnnnnnn nnnncgtgaa tgcacatatg
     1201 tttaccgaat ttattgcgac aggttatggt gataacagcg tatggggtga gaacactacc
     1261 gctgagaata agcagtcttt gcttgacgct tataattctt tctcctatga gaagtattga
     1321 ttatttaaaa attaataagt tttaactgca aaagggttca gccgtaaaaa gctgaaccct
     1381 tttattgcgc aacaaaaacg gtttagccaa attgactgaa ccgttaatct gtactatgtt
     1441 aagctcggga acaagtagtt gtaaattatc acaaactacc tacacgaact gcgggcgagg
     1501 tactcgcaaa aagggtagct tgaaaatcaa gctaccctaa attcgtacta tgtaatgctc
     1561 gggtgacaga agtttgaata tatcaaacta acttcacgaa ctaaaaaaca ttagttgttg
     1621 atgagcttat cttcgtcaga ccagctgtag agctttctaa cttccttacc gaatacttcg
     1681 gcaggagctt cggcagcgag ctttctcata gccttgaagt gaacctgtga accggcatcg
     1741 ctcatgtcaa ggaggaagtc cttagcaaat gtaccgtcct gaatgtcaga aagaaccttt
     1801 ctcatagctt tcttagtatc ctctgtgatg atctttggac ctgtgatgta gtcaccgtac
     1861 tcagctgtgt tagagattga atatctcatg cctgcaaaac ctgactgata gataaggtca
     1921 acgatgagct tcatctcatg gatacactca aagtaagcgt ttcttgggtc gtaacctgca
     1981 tcgcaaagtg tttcaaaacc tgcctgcata agtgcgcaaa caccgccgca aagaacagcc
     2041 tgctcaccaa agaggtctgt ttctgtttct gttctgaatg ttgtttcaag aacacctgct
     2101 cttgcgccgc caataccgag tgcatatgca agtgcgagat ccttagcctg acctgtagca
     2161 tcctgctcaa cagcaacgag gcaaggtaca cccttaccaa cctgatactc tgaacgaact
     2221 gtgtgacccg gtcccttagg
//
LOCUS       DAOMVE010000201         2208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_14532_length_2208_cov_1.790989, whole genome shotgun sequence.
ACCESSION   DAOMVE010000201 DAOMVE010000000
VERSION     DAOMVE010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14532_length_2208_cov_1.790989"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2208
                     /locus_tag="PLS64_08755"
     CDS             <1..>2208
                     /locus_tag="PLS64_08755"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HRL43945.1"
                     /translation="FSDKTTGDPSLGAGWATKNNRAIWINQDGKSEYEKLSKDSSGNY
                     FIKLGSDYTDNPNAAVTFYSCESTVTSNPQNSYVAKWTTTLQTAVDAESQTFTAYGYK
                     DSSNNGYGTWGEVQKILLSSEDASTLPMTQDDGKYLAVDMYVKDSSTPIAMTFEPNEK
                     ASLSGWVAYLPNPNSDAAHSITFKFTYKGKDYSISAPNRNSSVNYVITSQNTGYWAPP
                     AIVSVYSTCTDEKDNNAVMGTVSVTGGMDGATSVKVTKGTTVTLNAKPTDSNKYRFIG
                     WYSDPEFTAPVPLSNGAYQANDTSAEHKFYAKFQRQYKVEAKAVSDGAVANSTGGTVK
                     ISGGEEVAYAVGSYLEGQNTSITATPKEGYDFMGWYSDENCTKLESQDLTLSIKNIQA
                     NHLYYAKFMIKQFSVTAVANHPNDKKNSTVQFSSPLSEASDTRVTVKVNYNGSATFVA
                     NAGEGYEFVGWYSDENCQTLVSKTTPYKVSSIKADYTLYAKFKIINLNLKVYSVTEGE
                     INGAGGTVQLGADPAAAKIETTVEWGTLATLTAKANANYEFKGWFTDPQCSIKADSKI
                     LNNCQYTDKTVETAAIKKDLTLYAKFSDVSSRKVTANAVFGGNIVDTAGTVKAGDSQE
                     GATSTAVVTNGNSVTLVANTKPNYKFMGWYSDRECTNSVASEQQLVLTNVDAGCEYYA
                     LFKLQSFSVNAVVDGDSVGTVKFTAPKEVGPSTAVTVSVDYDGSATFVANPAEG"
BASE COUNT          684 a          463 c          514 g          547 t
ORIGIN      
        1 ttttccgata aaacaacggg cgatccatct ctcggtgcgg gttgggctac caaaaacaat
       61 cgtgcaatct ggattaacca ggatggcaaa agcgagtatg aaaagctttc aaaggattcg
      121 agcggtaact attttattaa gctcggttca gactatacag ataatcctaa cgcagcagtt
      181 accttctaca gctgtgaatc aaccgtaaca tcaaatccgc agaacagcta tgttgcaaaa
      241 tggacaacca ctctgcaaac tgctgttgat gccgaatcac agacattcac agcctacgga
      301 tataaggatt cgtcaaataa cggctacggc acatggggag aggttcagaa aattctcctc
      361 tcaagtgaag atgcaagtac attgccgatg acacaggatg acggaaagta tcttgcggtt
      421 gatatgtatg ttaaggacag ttcaactccg atagcaatga cttttgaacc gaatgaaaaa
      481 gcgtcactca gcggttgggt tgcatatttg cccaatccta acagcgatgc cgcacattcg
      541 attacattca aatttactta caaaggcaaa gattacagca tcagcgcacc gaacagaaat
      601 tcttcggtga actatgttat tacttcacaa aacacaggct actgggcacc tccggctatt
      661 gtttccgttt attctacatg tacggatgaa aaggataata atgccgtaat gggaacagta
      721 agcgtaacag gcggtatgga cggcgcaaca agcgttaagg ttacaaaggg tacaaccgtc
      781 actcttaatg caaaaccgac cgacagcaac aaatacagat ttatcggttg gtactctgac
      841 cccgaattta cagctccagt tcccctcagc aacggtgctt accaggcaaa tgatacatca
      901 gccgagcata agttttatgc gaaattccaa agacagtaca aggttgaggc aaaagctgtt
      961 tcggacggag cagttgcaaa cagcacaggt ggtactgtta agatttcagg cggcgaagag
     1021 gtcgcatacg ctgtcggttc gtaccttgag gggcagaata catcaatcac agccacacca
     1081 aaagagggct atgacttcat gggttggtat tctgacgaga attgcacaaa actggaaagt
     1141 caggatctta cgctttcaat taagaatatt caggcaaatc atttgtatta tgccaaattt
     1201 atgattaagc agttctcggt aacagcggtt gcaaatcatc cgaatgataa gaaaaacagc
     1261 acagtacagt tttcttcacc gttatccgag gcgtctgata caagagttac cgtcaaggta
     1321 aactacaacg gttctgctac ttttgttgcg aatgcaggcg agggttatga gtttgtcggc
     1381 tggtattcgg acgaaaactg tcagacactt gtcagtaaaa cgactccgta caaagtaagc
     1441 agcataaagg ctgactatac tctgtatgca aaattcaaga ttatcaatct taatctcaaa
     1501 gtttattcag taacagaggg agaaatcaac ggagcgggcg gtacggttca gcttggagct
     1561 gatcccgctg ccgcaaagat tgagacaacc gttgagtggg gtacgcttgc cacactcacg
     1621 gctaaagcaa atgccaacta tgagtttaaa ggttggttta ccgatccgca atgcagtata
     1681 aaggcagaca gcaagattct taacaattgt cagtacacag ataagacagt ggaaactgcg
     1741 gcaataaaga aggatttgac tctgtatgca aagttttcgg atgtatcatc tcgcaaagtt
     1801 accgctaatg ccgtgttcgg cggaaatatt gtcgatacag caggtactgt taaggcaggc
     1861 gattcgcagg aaggtgcaac atcaactgcg gtggttacca acggtaatag cgtaactctt
     1921 gttgcaaata caaagccaaa ttataagttt atgggttggt attccgacag agaatgtaca
     1981 aactctgttg catcagaaca acagcttgtt cttacaaatg tcgatgccgg ttgcgaatac
     2041 tacgcattgt ttaagctgca gtcgttttca gtcaacgcag ttgttgacgg tgattcagta
     2101 ggtacggtaa agtttaccgc acctaaagaa gtcggaccgt caactgcagt tacagtttcg
     2161 gtggattatg acggaagtgc aacatttgtt gcaaaccctg ccgagggt
//
LOCUS       DAOMVE010000202         2196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_14637_length_2196_cov_3.620271, whole genome shotgun sequence.
ACCESSION   DAOMVE010000202 DAOMVE010000000
VERSION     DAOMVE010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14637_length_2196_cov_3.620271"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLS64_08760"
     CDS             <1..346
                     /locus_tag="PLS64_08760"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HRL43946.1"
                     /translation="ADFGFMSEPDITALLDNLLENAVEAAQNTENGFIDFSAFVRKNK
                     YLSIQLTNSVKNIVAVKNNRIASSKKSDAIHGTGLKSIKRVVKKYDGEINMKFNEDDM
                     TFTSNIVFEIRR"
     assembly_gap    486..495
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(683..2091)
                     /locus_tag="PLS64_08765"
                     /pseudo
     CDS             complement(683..2091)
                     /locus_tag="PLS64_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          616 a          415 c          341 g          814 t
ORIGIN      
        1 tgctgatttt ggctttatga gtgaacccga cataactgct ctgcttgaca atcttcttga
       61 aaacgcagtt gaagcggcac aaaatacaga gaacggattt atagattttt cggctttcgt
      121 ccgcaaaaat aagtatctgt caatacagct tacaaactcg gtcaagaata ttgtagccgt
      181 caagaacaac cgcatagcat catcaaaaaa atctgacgca atacatggaa cgggattgaa
      241 aagcattaaa agagtagtta aaaaatatga cggtgaaata aatatgaaat tcaatgagga
      301 tgatatgacc ttcacctcaa acattgtgtt tgaaattcgg agataacgca gtaatcgaat
      361 catataattc cttgactaat gaaatgcatt atataatgag aaattccaat acctaataat
      421 actgatatca acaagatgag atctaattga tggaattaag aatcggaaag aaattgcaaa
      481 tataannnnn nnnnnctatt aaacatttta tatagcaagc tacttttacc cccatgttca
      541 cggtatctct gcttggtttc gattagtcca acttttttta aatctttaat agctcttctt
      601 actgtggcag gcgacagctt tatgtggtga cccccaaaag ttagatttga aagcgtagtg
      661 gcttttagct gctacgcttt tgttatgcaa ctaaggcatt aatcctgtac tcaaccggac
      721 ttaaccagcc gagtttttgt ttgattctct tgttgttgta gtaatcaatg tatttgataa
      781 ttgcttgttt aagctcttca tagctgtaat aaacttttcc ataatatatt tcttgcttta
      841 atattccaaa gaaattttcc ataacagagt tatcgtgaca atttcctttt cttgacatac
      901 tctgaaatat tctgttttct ttcagctttt gtgaatatgc tttcatctga taagcccatc
      961 ctctgtcaga gtgaaaagtc ctgcgatatg aacagtcatt agtgatagta atagcttcat
     1021 ctaatgcact cataatgttc tcggcagatg gcttttgaga tatgccaaaa ctcagaattt
     1081 ctccgttaaa catatccata aatggatcaa aatagagctt tttgattatc attctgccct
     1141 tgctgtcaac ctcataatac ttaaattcac ttgtatctgt tgtgattttc tgatgcggta
     1201 cacttgtgtt aaatctacgg ttaatcctgt ttggagcaat ctttcctact ttacctttgt
     1261 atgaactgta ttttctgctt ttatgagtaa acgaggttac ttgcaggttc aatttctgaa
     1321 caattctttg aacttttttc ttgtttacaa aaatatttct gtttcgcaat tctccccaaa
     1381 ttctgcgata accatagtct ttatgcactt ttcgtatatc aagaatttct ttttctaatt
     1441 tttcatccgg gttctctcgc tcaaatctat tttgccaata catatatgtt gactttggaa
     1501 aacctgttac ggcaagaata tcttttagcc tgaattctcc tcggagactg tgtattattc
     1561 ttgcctgttt ttcggaggtt tttcttcctc caaacgcaat ctcctcagct cttttaaata
     1621 cgcattttca atccttaatc ttaaattctc atcttcaagc tgtttcagat attctgattc
     1681 agctttttct tttcctgtgt tgggtattat cttttttggt ttagacactt taggtttccg
     1741 tccttttctc tttggtcgta acgcatcagg tccaactgca cggtaatcgg ttacccattt
     1801 tgctatcatt ggtggattat tgatacctaa actcaatgca agttctcgat acgatacttc
     1861 cgttgttaag tataattcta ccacatgcat cttaaattca aaagagtaat ttttcttttc
     1921 tcttgatcgc attaatcctt tgtttccaaa ttctttgtat gctgagatcc aatctcgtaa
     1981 tattgtttga ctcggtatcc cattttcttt acataaactt ttatatcctc cttttccatt
     2041 tatgtagtcc attactacct tcttcttgaa ttcataactg tattttgaca taaaaatacc
     2101 gacctccaaa gttagatttt ttaggtctaa cttttggggg tcgggtcaac acaaaaggtc
     2161 aatgagcggt ttatattttt ttaagcggaa tatttt
//
LOCUS       DAOMVE010000203         2182 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_14769_length_2182_cov_3.102492, whole genome shotgun sequence.
ACCESSION   DAOMVE010000203 DAOMVE010000000
VERSION     DAOMVE010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14769_length_2182_cov_3.102492"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1168
                     /locus_tag="PLS64_08770"
     CDS             227..1168
                     /locus_tag="PLS64_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43947.1"
                     /translation="MRKLNFTKALKNALPYVLTLILVGIMTFVAEILGEGEIIFPEIT
                     ALAIGYMVTQKQGWKVNDSRMIALIAICAVAGVLTVRYIKVGLYLEILIAFVFAQILF
                     MFSGTTFAPMISAIVLPVMMQTESFIYPIAAFLLTIAVVLFRRFLLKVKIRDKEDFQS
                     VNLHAKSDVIDTAVRTICVAVVGFFAIWSGFKFAIAPPLLVAFTEFSRPKNKARNKPV
                     KTVLVITVCAFVGAVSRYVLNMTLGLPLTVAALTATAIMLVIVYCTKMYIPPAGALTI
                     LSMIIPSESVLLYPLQILVGTAVIMLISRLLFMKRQN"
     gene            1211..1990
                     /locus_tag="PLS64_08775"
     CDS             1211..1990
                     /locus_tag="PLS64_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRL43948.1"
                     /translation="MDIFITILVTFFAGMGAGLGTGFAGMSAAAVISPMLITFLNMDP
                     YMAVGIALSSDVLASAVSAYTYHKNKNLDVRNGLIMMASVLAFTIVGSYVASIVPSAT
                     MGGFSVFMTFLLGIKFIIRPVMTTKEAMLGVSAKKRAIQSVICGMLIGFICGFIGAGG
                     GMMMLLILTTVLNYELKTAVGTSVFIMTFTALTGAASHFMIGGTPDWFVFILCIAFTL
                     IWARIAAVFANKAKPKTLNRATGIVLVVLGVVIMGFKLLAK"
BASE COUNT          610 a          381 c          500 g          691 t
ORIGIN      
        1 aaaccgtagg tgacttaaaa aacgccgcta atttctgcac cgtttttatc atatgctgaa
       61 cctccaatat gtacagcgtt ttttgcaaga cctgcatatc cacgcataat gacagatacg
      121 ctgttaaatg aaaaatctgt gaataaattt tgtctgttaa tgctgacaga tttatgatac
      181 aatagtgctg ttagtttgaa ttgaaaagtg agtagaagta taaaaaatga ggaaactgaa
      241 ttttacaaaa gcacttaaaa acgcattacc gtatgtatta acgcttatac ttgtcggtat
      301 aatgactttt gttgcggaaa tattgggtga gggggagata atctttcctg aaataacggc
      361 acttgcaatt ggttatatgg ttacacaaaa gcaaggttgg aaagtcaatg acagccgaat
      421 gattgcgtta attgcgattt gcgctgttgc gggtgtgctg acagtccgat acataaaagt
      481 cgggctttat cttgaaatat tgattgcatt tgtatttgca caaattctct ttatgttttc
      541 gggaacaaca tttgcaccga tgatttcggc aattgtgttg cccgttatga tgcagaccga
      601 gagctttata tacccgattg ccgcattttt gctgacaatt gcggttgtat tattccgcag
      661 atttcttttg aaagttaaaa taagagataa agaggatttt cagtcggtaa acctgcatgc
      721 aaagagcgat gtgattgaca cagccgtaag aacaatctgt gttgccgttg tgggattctt
      781 cgcaatttgg tcgggcttta aatttgcaat tgcaccgccg ttgcttgtgg cgtttacgga
      841 attttcccgt ccgaaaaata aggctcgcaa caagccggta aagacggttt tggtgataac
      901 agtctgtgca tttgtcggag cggtttcaag atatgtgctg aatatgactc tcggattgcc
      961 gttgacagtt gccgcattga cagccacggc gataatgctt gtaattgttt actgcacaaa
     1021 aatgtatatt ccgcctgccg gagcattgac aattttgagt atgattattc cgagtgaaag
     1081 cgtacttttg tacccgttgc agatacttgt cggaacggcg gtaataatgc tgatttcacg
     1141 gcttttgttt atgaaacggc aaaattaaaa taatcttaat ttagttatat tattctatag
     1201 aggaaattaa atggatatat ttattacaat tttagttaca ttttttgcag gaatgggcgc
     1261 aggtctcgga acaggttttg caggaatgag tgcggcggct gtaataagtc cgatgctcat
     1321 aacttttctt aatatggatc cgtatatggc agtcggaatc gcactttcgt ccgatgtttt
     1381 ggcgagtgca gtttcggctt atacatacca taaaaataaa aatctcgatg tcagaaacgg
     1441 tttgattatg atggcgagtg tgcttgcgtt tacgattgtc ggcagttatg ttgcaagcat
     1501 tgttccatca gctacaatgg gcggattttc cgtgtttatg acatttttgc tcggcataaa
     1561 atttatcatc cgtcccgtta tgacaactaa agaagctatg ctcggtgtgt cggcaaaaaa
     1621 gagggcaatc cagtctgtaa tctgcggaat gttaatcggc tttatctgcg gatttatagg
     1681 tgcgggcgga ggaatgatga tgcttttgat tttgacgacc gtgctgaatt atgaattgaa
     1741 aaccgctgtc ggcacaagcg tattcattat gacattcacc gccctgacag gcgcagcttc
     1801 gcactttatg attggtggca cacccgattg gtttgtgttc attctctgca tagcgttcac
     1861 ccttatatgg gcgagaattg ccgccgtctt tgcaaacaaa gctaagccca aaaccctgaa
     1921 ccgtgcaaca ggcattgtcc tcgtggtgtt aggtgttgtt attatgggct ttaagctttt
     1981 agcaaaataa aaattgacag tttaaaatta aaaattcagg tcggcaatat atttgtaaaa
     2041 tgcaaaattt ctgttgcccg atttttcaac ggttatgttg gggctaatcg agtagggcga
     2101 aacaaatgct cgctttgaac aatattcagg gggaagtatg cttttttaca attgcattat
     2161 tatttcgcaa aaaacaaagg ag
//
LOCUS       DAOMVE010000204         2153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_15059_length_2153_cov_4.405148, whole genome shotgun sequence.
ACCESSION   DAOMVE010000204 DAOMVE010000000
VERSION     DAOMVE010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15059_length_2153_cov_4.405148"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..392
                     /gene="rbfA"
                     /locus_tag="PLS64_08780"
     CDS             15..392
                     /gene="rbfA"
                     /locus_tag="PLS64_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009061868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRL43949.1"
                     /translation="MGSHRIERTTEDIKRELTAIFRELKDPRVQQAFISIVRVEVTND
                     LSYCTVQISAIEGLDRAKEAVKGLKSASGFIRRELGHRLHLRHVPELIFHATDSIEYS
                     ANISRILNSLDIKEDEEENDESV"
     gene            376..1317
                     /locus_tag="PLS64_08785"
     CDS             376..1317
                     /locus_tag="PLS64_08785"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRL43950.1"
                     /translation="MMNLYEVARFLEAHDNYDILTHAYPDGDTLGSGFALCLALRQIG
                     KKARVITTGVPTKFTYLQKNIEEEDFTTETVVSVDVAADSLLGSNMGKYIGKVDLCID
                     HHGSNTFTAKEKFVDKYAAANCEILYKLFLRMHINFTKDIADCLYTGISTDTGCFRYT
                     NTTAETMRVAASLMDFGCNTAYINKAMFETKSKNKIQLERAVYESMTYCADGRCAIIY
                     TTLDMLKKLNTGDDEMEGLASIPRQIEGVLIGITMREKEGGFFKISVRTNNNINASDF
                     CSQFGGGGHPAAAGCSIEGDLETVKTTLEKAAEKFLS"
     gene            1314..>2153
                     /gene="truB"
                     /locus_tag="PLS64_08790"
     CDS             1314..>2153
                     /gene="truB"
                     /locus_tag="PLS64_08790"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRL43951.1"
                     /translation="MSNLNGVLLIDKPKEFTSFDVIAVVRRLTGQRKLGHTGTLDPNA
                     TGVLPVLLGTATKTQDLILNHDKSYTAEFQLGKTTDTLDIWGTVTGECESSVTEEQLR
                     RVIPNFTGEIEQIPPMYSAVQKNGQRLYDLARQGIEVERESRKVTVYSLSLANFDEKA
                     QTGTLEISCSKGTYVRTIIDDIGKVLGVGAVMTALCRTSACGFSLDECITLDELKSIC
                     ENGNVEEKLRSVESLFMSLGYLAVSSAQAKRFANGGALSADRTYLAKSGYENGQLFRM
                     KTHD"
BASE COUNT          633 a          480 c          524 g          516 t
ORIGIN      
        1 aaggatgtga tagcatggga agccatagaa ttgaaagaac aacagaggac atcaaaagag
       61 agcttactgc tattttcaga gaattaaaag acccacgggt tcagcaggcg tttatctcta
      121 tcgtaagagt tgaggttaca aacgaccttt catactgcac ggtacagatc agcgcaattg
      181 aaggtcttga cagagccaaa gaggctgtca agggcttaaa gtctgcatcg ggctttatca
      241 gacgagagct tggacacaga cttcatcttc gtcatgttcc cgaactcatt ttccacgcaa
      301 cagacagcat agaatacagc gcaaacatca gccgtattct caacagcctt gacattaagg
      361 aagacgagga agaaaatgat gaatctgtat gaggttgccc gttttcttga ggcacacgat
      421 aattatgata ttctgactca cgcttatcct gacggtgaca ccctcggcag cggatttgca
      481 ctctgccttg cgctccgcca aatcggcaaa aaagcaagag ttatcacaac aggtgttccg
      541 acaaagttta cctatcttca gaaaaacata gaggaagaag attttacaac cgaaacggtt
      601 gtcagcgttg atgttgcggc tgacagcctt ctcggttcaa atatgggcaa atatatcggc
      661 aaggttgatt tgtgcattga ccaccacggt tcaaacacat ttaccgctaa ggaaaagttt
      721 gttgacaaat atgcggcggc aaactgcgaa attctctaca agctgttcct cagaatgcac
      781 atcaacttta caaaggacat tgcagactgc ctctacacag gcatttcaac cgacaccggc
      841 tgtttcagat acacaaacac aaccgccgaa accatgcgtg ttgccgcaag tcttatggac
      901 tttggctgta acaccgcata cataaacaag gcgatgtttg aaacaaagtc gaaaaacaaa
      961 attcagcttg aaagagccgt ttatgagtca atgacctatt gtgcagacgg caggtgcgca
     1021 ataatttaca caacgcttga tatgctcaaa aagctcaaca cgggtgatga cgaaatggag
     1081 ggacttgcct ctattccccg tcagattgag ggtgttctca tcggaattac aatgcgtgaa
     1141 aaagagggcg gattcttcaa gatttccgtt cgtacaaaca ataatatcaa tgcctcggat
     1201 ttctgttcgc agttcggagg cggcggtcat cctgcggcgg caggctgctc aattgagggc
     1261 gaccttgaaa ccgtaaagac aacgcttgaa aaagctgcgg agaagttttt atcatgagca
     1321 atcttaacgg tgtactgctg attgacaagc cgaaggaatt cacctcgttt gatgttattg
     1381 ccgttgtccg cagactgaca ggacagagaa agctcggtca cacgggaaca cttgatccga
     1441 acgcaacggg cgttctgcct gttttgctcg gaactgcaac gaaaacgcag gatttgattc
     1501 tcaatcacga caagtcatac acggcagaat ttcagcttgg caaaaccacc gacaccctcg
     1561 acatctgggg tacggtaacg ggagaatgcg agtcttcggt tacagaggag cagcttcgca
     1621 gagtaattcc caatttcacg ggcgaaattg agcagattcc gccgatgtac tctgccgtac
     1681 aaaagaacgg tcaaaggctt tatgaccttg caagacaggg aatcgaggtt gagcgtgaga
     1741 gccgaaaggt tactgtctat tctctttcgc ttgcgaattt tgacgaaaaa gcacagacag
     1801 gcacgcttga aatttcctgt tcaaagggta cttatgtacg cacgataatt gatgacatcg
     1861 gcaaggttct tggtgtcggt gcggttatga cggcactttg cagaacctcg gcttgcggat
     1921 tttcgcttga cgagtgcata acgcttgacg agctgaaaag catttgcgaa aacggaaatg
     1981 ttgaagaaaa actgcgttcc gttgagagcc tgtttatgag tttgggctat cttgccgtat
     2041 cgtctgcaca ggcaaaacga tttgcaaacg gcggtgcgtt gagtgccgac agaacatatc
     2101 ttgccaagtc ggggtacgaa aacggtcagc ttttcagaat gaaaacccat gat
//
LOCUS       DAOMVE010000205         2142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_15174_length_2142_cov_3.908002, whole genome shotgun sequence.
ACCESSION   DAOMVE010000205 DAOMVE010000000
VERSION     DAOMVE010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15174_length_2142_cov_3.908002"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1675)
                     /locus_tag="PLS64_08795"
     CDS             complement(233..1675)
                     /locus_tag="PLS64_08795"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HRL43952.1"
                     /translation="MKKENMAMLCDFYEFTMSNGYFKNGFYKKNVYFDVFFRKVPDNG
                     GFAIMAGLEQAIEYIRELHFEDEDIEYLKSKGIFDEQFLEYLRNFKFSGDVYAIPEGT
                     PIFPNEPVMTIKAPAIEAQLVETFLLLTINHQTLIATKANRIVRAAQGRAVLEFGSRR
                     AQGANAAVDGARAAYIGGCKGTACTLTDELYGVPAGGTMAHSWVQMFNSEYDAFKTYC
                     EMYPDNPTLLVDTYNTLKSGVPNAIKVFKEVLLPQGKTKCAIRLDSGDISYLSKKARK
                     MLDDAGLTECTITASNALDEYLIRDLMMQGAQVDTFGVGERLITSSSSPVFGGVYKLV
                     AVENDGGEIVPKIKVSENTTKITNPHFKKVYRYFDKDSGKAIADELCIYDEVVDDSKP
                     RTIFDPNAVWKTKMLDNYTAKELLVPIFKNGKCVYESPSIEEIATYCREQIDLLWDEV
                     KRFENPHNYYVDLSKKLYDIKALLLNIYEK"
     gene            1886..2053
                     /locus_tag="PLS64_08800"
     CDS             1886..2053
                     /locus_tag="PLS64_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43953.1"
                     /translation="MNSENNKLCPFCESEMMIIQDNLNNGKPYLECPTCGLRFQIEGF
                     PENPQEIRETE"
BASE COUNT          644 a          471 c          412 g          615 t
ORIGIN      
        1 tttcaatggt aaaaacaagc aaaaatcccg acagcacaaa acctgtcggg attttttctt
       61 tacactaatt atatcaaaaa gcactgtacc attatccacc ctttccttta caaaaccatt
      121 tgttatgatt atttaattta gccatgacca aaaaacagtc ggatagacga actacccgac
      181 cgtaaatttt acttttcaat tttcagattt ttaattaaaa aataatcaaa aatcactttt
      241 catagatatt gagcaaaaga gccttgatgt catagagttt ctttgagagg tcaacataat
      301 agttatgcgg attttcaaaa cgcttaacct cgtcccagag aaggtcaatc tgctcacggc
      361 aataggttgc gatttcctca attgacggtg actcatacac gcacttgccg tttttgaaga
      421 ttggcacaag aagttccttt gcggtatagt tatcaagcat ttttgttttc caaactgcat
      481 tcggatcgaa aattgttctc ggctttgaat cgtcaacaac ctcatcataa atgcaaagct
      541 catcggcaat agccttaccg ctgtccttgt caaaatagcg gtaaaccttt ttgaagtgcg
      601 gatttgtaat cttggttgta ttttcgctga ccttgatttt cggcacaatt tcaccgccgt
      661 cattttcaac ggcaacaagc ttataaacac cgccgaatac aggcgaactt gatgaagtga
      721 tgagtctttc accgacaccg aatgtgtcaa cctgagcgcc ctgcatcata aggtcacgaa
      781 taaggtactc gtcaagagcg tttgacgcag taattgtaca ttcagtaaga cctgcgtcat
      841 caagcatttt tcttgctttc tttgaaagat aggaaatatc gcccgaatca aggcggattg
      901 cacacttggt cttaccctgc ggaagaagaa cctccttgaa caccttaatg gcattcggga
      961 caccgctctt taatgtgttg tatgtatcaa caaggagagt aggattatcg ggatacatct
     1021 cgcagtaggt tttaaaagcg tcatactcgc tgttgaacat ctgaacccat gaatgtgcca
     1081 ttgtaccgcc ggcgggaaca ccgtaaagct cgtctgtaag tgtgcaggct gtacccttgc
     1141 agccgccgat atatgcggct cttgcaccgt caactgcggc atttgcaccc tgagcacggc
     1201 gtgaaccgaa ttcaagtacg gctctgccct gagcggcacg gacaattctg ttagcctttg
     1261 tcgcaatgag agtctgatga ttgattgtga ggagaaggaa tgtttcaaca agctgagctt
     1321 caattgcagg cgctttgatt gtcataacag gctcgttcgg gaaaatcgga gttccctcgg
     1381 ggattgcata aacatcaccg ctgaatttga agtttctcaa atactcaaga aactgctcgt
     1441 caaaaatgcc cttgctcttg agatattcga tatcctcgtc ttcaaaatga agttccctga
     1501 tgtactcaat agcctgttca agacctgcca taatcgcaaa accgccgttg tcgggaactt
     1561 tgcggaagaa cacatcaaaa tagacattct ttttataaaa accgttttta aaatagccgt
     1621 ttgacattgt aaattcgtag aagtcacaca gcatagccat attttctttt ttcataataa
     1681 tcaccttgaa tgagattgta tattctgcaa cgcagaagca tagatatagt ttagtctttt
     1741 ttcgtcaatt tgtaaagcgg aatttgcaca aaagccgtta aaatccaacc ctcttgccta
     1801 tacattttta atatctcggt tgacatttta ccgcttaaag gtataattaa gttatatcga
     1861 aaacaatcca ctcggaggta aaaatatgaa cagcgaaaat aataaactct gcccattctg
     1921 cgaaagcgaa atgatgatta ttcaggacaa cctcaacaac ggcaagccgt accttgaatg
     1981 ccccacctgc ggactccgtt tccaaattga aggcttcccc gaaaatccgc aggaaatcag
     2041 agaaactgag tgatttggtc tgaacatttg tattgcagat attgaaaaaa tcaccgctcg
     2101 gctcaaaatg tcaaatttca caaaaatgtg ttgacaaatc ga
//
LOCUS       DAOMVE010000206         2139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_15201_length_2139_cov_2.135797, whole genome shotgun sequence.
ACCESSION   DAOMVE010000206 DAOMVE010000000
VERSION     DAOMVE010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15201_length_2139_cov_2.135797"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PLS64_08805"
     CDS             <1..512
                     /locus_tag="PLS64_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43954.1"
                     /translation="WANIKNMQEVSKLINFLSENNMRSESDIENRAAEKYNDRMVIVS
                     KLNRTQNQINDIADVIKLIRTYEKYKPYHKNLMTAKNQKQYKKENITALAKYDDAVAK
                     LLSLYPDRKLPTISTLEEKRKKLTSDVQQMNEEYKAIIDELKKIEYARQSINDYLRNN
                     DRSQNKSLE"
     gene            complement(516..956)
                     /locus_tag="PLS64_08810"
     CDS             complement(516..956)
                     /locus_tag="PLS64_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43955.1"
                     /translation="MVTIKEFTCERCGNKFETTAKHAKYCPECREIKKLERIQAHKEK
                     QKAGTARRVGTEQICPNCGKSFILKSGSQKICEECAKKRVYPKNSTVKPKDLYDIIHI
                     YVPKGEKEKLQNWVKDKGLNLNKAYNYAMQLFMEEIDREEEKYK"
     gene            1162..1320
                     /locus_tag="PLS64_08815"
     CDS             1162..1320
                     /locus_tag="PLS64_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43956.1"
                     /translation="MKTVRVVEYIDLEGENHTDEFEESADAVKFFMRITSEKLVKRAA
                     LYDKVIEN"
     gene            1380..1805
                     /locus_tag="PLS64_08820"
     CDS             1380..1805
                     /locus_tag="PLS64_08820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRL43957.1"
                     /translation="MIRNEVKLIGRLTADPELKYTKETNKALCNVVLAVNSIYSNEAE
                     YLHLVFWNKTAENLCKYKGKGDLIAVTGYLKTRSYIDKENIVRNIVEVIVEQVDYLGY
                     TKRGVDKSEPIPKLDPDHKKQEEYQMIHDALCADEDLPF"
     gene            1818..>2139
                     /locus_tag="PLS64_08825"
     CDS             1818..>2139
                     /locus_tag="PLS64_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43958.1"
                     /translation="MKFKYKVEIVIDEEKVINDDIYDPKEMYEFIRNMFKRFDLAEIK
                     TDKPYYLIFADKGRSRDYGALWRVILDLYDMDWFKNYAVKYFWHNKSNGSIEDIMLGL
                     NKCRK"
BASE COUNT          773 a          346 c          390 g          630 t
ORIGIN      
        1 attgggcgaa tattaagaat atgcaggagg tttcaaagct cataaatttt ttgtctgaaa
       61 acaatatgag aagtgagtcg gatatagaaa acagagctgc agaaaaatac aatgacagaa
      121 tggttattgt ttccaaactt aacagaactc aaaaccaaat taacgatatt gcagatgtaa
      181 taaagctcat aaggacatat gagaagtaca agccttatca caaaaatctg atgactgcaa
      241 aaaatcaaaa acaatacaaa aaagaaaaca tcaccgcact tgcaaagtac gatgatgcag
      301 ttgctaaatt attaagtttg tatcccgaca gaaaattgcc gacaatttca actcttgaag
      361 aaaaacggaa aaaattaact tccgatgttc agcagatgaa tgaagaatac aaggcaatta
      421 ttgatgagct taaaaagatt gaatatgccc gtcaaagcat taacgattat ctcagaaaca
      481 atgacaggtc acaaaataaa agcttggaat aattattatt tatatttttc ttcttcacgg
      541 tcaatctctt ccataaataa ttgcattgca taattatatg ccttgttaag atttaaccct
      601 ttgtctttga cccaattctg caatttttcc ttttcgccct tcggcacata aatatggatt
      661 atgtcatata agtccttcgg ctttaccgta gaatttttag gataaactct cttctttgca
      721 cattcctcac agattttctg actgccggat tttagaatga aggattttcc gcagttcggg
      781 catatctgtt ccgtgccgac tcttcgtgcc gttcctgctt tttgtttttc cttgtgtgcc
      841 tgaatccgtt cgagcttctt tatttctctg cactcaggac aatatttagc gtgtttagcg
      901 gtggtttcaa atttatttcc gcatcgttcg caggtaaatt ctttaattgt aaccatcaga
      961 ccaatccctt tcgctatact tttgctacta ctattatttt atcaagtata aacaaattgt
     1021 caataaaaat tgaaaaaaat gccgtttttg aacaaaaaat ttaaaattac acttgacatt
     1081 acccctaaat gaagtataat ataatcagaa atagtttagg aaatgatttc aagtgtgaaa
     1141 agttaaggag gtaaaacaaa gatgaaaaca gtaagagtag ttgaatatat cgaccttgag
     1201 ggagaaaatc acaccgatga atttgaagaa tcagccgatg cggttaaatt ttttatgagg
     1261 attacatcgg aaaagctcgt aaaaagagct gcgttatatg acaaagtaat tgaaaattaa
     1321 cttgtgcccc aacggggcag aagttgcaaa acacaaatat ttttaaagga gttaaaatta
     1381 tgattcgcaa tgaagtaaaa cttatcggca gacttaccgc cgaccctgaa ctcaaataca
     1441 caaaagaaac aaacaaagct ctgtgcaatg tagtgttagc tgttaacagt atttacagta
     1501 atgaagcaga gtatttgcac cttgtctttt ggaacaagac ggctgaaaat ctttgcaaat
     1561 ataaaggcaa aggtgatttg attgctgtta caggatacct taaaacgagg agctacatag
     1621 acaaagagaa tattgtcaga aatattgtag aagtcattgt tgaacaagtt gactatctcg
     1681 gttacaccaa aagaggagtt gacaaaagcg aaccaatacc gaagcttgac cctgaccaca
     1741 agaaacagga agagtatcag atgatacacg atgcactgtg tgctgacgaa gacttaccgt
     1801 tttaaggagt gatataaatg aaatttaaat acaaggtcga aattgttatt gatgaagaaa
     1861 aggttatcaa tgacgatatt tacgacccta aagaaatgta tgaattcatc aggaatatgt
     1921 ttaaacgctt tgacttagct gaaattaaaa ccgataaacc ctattacttg atttttgcag
     1981 ataaaggcag aagcagagat tacggtgcat tatggagagt aattcttgat ttgtatgata
     2041 tggattggtt taaaaattat gccgtaaaat atttttggca taacaagtca aatggcagca
     2101 tcgaagatat tatgttaggt ttaaacaaat gcagaaagg
//
LOCUS       DAOMVE010000207         2111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_15462_length_2111_cov_3.262646, whole genome shotgun sequence.
ACCESSION   DAOMVE010000207 DAOMVE010000000
VERSION     DAOMVE010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15462_length_2111_cov_3.262646"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLS64_08830"
     CDS             complement(<1..213)
                     /locus_tag="PLS64_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HRL43959.1"
                     /translation="MLDKIALTLLIIGGLNWGSVGIFQFDLVAWICGGQTGIISRIVY
                     ILVGLSAIWCITLLFRDTSDTVTDGTV"
     gene            complement(280..660)
                     /locus_tag="PLS64_08835"
     CDS             complement(280..660)
                     /locus_tag="PLS64_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43960.1"
                     /translation="MKKCEFCGKQISYFDQYCDDECHIKANKFYEKGERFGKLFAVIN
                     MICVFGIPIGLFMLSFAREVGLTVALASCVILGIMLIILPFPTENMISKFKIQKAVKI
                     TRMIGGAVIFLGVLILLLALLIKF"
     gene            810..1748
                     /locus_tag="PLS64_08840"
     CDS             810..1748
                     /locus_tag="PLS64_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HRL43961.1"
                     /translation="MESFFVALNAVLPMFIMLFIGFLVRKLKILQDSFLPQLNKIVFN
                     VFFPFLMFNNIYGSDFSSVFSPKLLAFAVIGVFTIYFLSIGFTLLVEKSNYSRGAMIQ
                     AIYRSNFVLMGLPIAANIFGKDKLGMTAVLVAVVVPIYNMLAVITLEIFRGQKINVLK
                     ILKGIAKNPLIIGSVLGLLSVAFNISLPETIDKTVSDIAAVATPLALIVLGASVTFDS
                     IKGCSRNLIICIIARLVVVPALCLGTAALIGIRGVDFVSLIGLFASPCAVSSFTMAQQ
                     MDSDFKLAGAAVVFTSVLACFTMFLWIFFFRQAGFF"
BASE COUNT          585 a          421 c          416 g          689 t
ORIGIN      
        1 aactgttccg tccgtcacgg tgtcgcttgt gtccctgaat aaaagtgtaa tgcaccagat
       61 tgcggaaagt ccgacaagga tataaacaat acggcttatg attcctgtct gtccgccgca
      121 aatccacgca acaagatcaa actgaaaaat gccgacactt ccccagttga gtccgccgat
      181 aataagaagc gtcaatgcta ttttatcgag cattcaaagt cctcctttgt atttgctgaa
      241 aatagtattg acgcaatttt tcgttttatt caatttaaat cagaatttaa ttaacaatgc
      301 aaggagcaaa atcagcactc cgaggaaaat taccgcaccg ccgatcattc ttgtgatttt
      361 tacggctttc tgaattttga attttgaaat catattttcc gtgggaaacg gaagaataat
      421 gagcataatt ccgagaatta cacagcttgc aagtgcgacg gtaagaccga cctctcttgc
      481 aaacgagagc ataaaaagtc cgattggaat tccgaacaca caaatcatat tgattaccgc
      541 aaacagcttg ccgaaacgct cgcccttttc gtaaaattta tttgctttaa tatgacactc
      601 atcatcacaa tactggtcaa aatacgatat ctgttttccg cagaattcac attttttcat
      661 ggacacacct cgttgtttca ttttaaatcg ttcacagaat attttaacac acaaatcgac
      721 atatttcaac ttgaacaaat gtacattttc gggtatatgc tttgttgaaa aaatttttgc
      781 aatgtggtat gctgaaaaga ggtgatttta tggagagctt tttcgttgcg cttaatgcgg
      841 ttctgccgat gttcataatg cttttcatcg gttttcttgt gagaaaatta aaaattttgc
      901 aggacagctt tctgcctcag cttaacaaaa ttgttttcaa tgtatttttt ccgtttctga
      961 tgttcaataa catctacggc tcagattttt caagcgtttt cagcccaaag ctgcttgcct
     1021 ttgcggttat tggcgtgttc acgatttact ttttgtcaat cgggtttaca ctccttgttg
     1081 aaaagtcaaa ttacagccgt ggtgcgatga ttcaggcgat ttaccgcagt aattttgtcc
     1141 ttatgggact tccgattgcg gcgaatattt tcggcaagga taagcttggc atgacagccg
     1201 tgcttgttgc tgttgttgtt ccgatttaca atatgcttgc cgtcatcacg cttgaaattt
     1261 tcagagggca aaagataaat gtgctgaaaa ttttaaaagg cattgccaaa aatccgctga
     1321 ttatcggctc tgtactcgga cttttgtctg ttgcgttcaa tataagcctt ccggaaacaa
     1381 ttgacaaaac ggtttcggat attgcggcgg ttgcaactcc gcttgcgctg attgtactcg
     1441 gtgcgtctgt cacatttgac agcataaaag gctgttcacg caacctcata atctgcataa
     1501 tcgcaagact tgttgttgtg ccggcacttt gtctgggtac ggcggcgctt ataggaatca
     1561 gaggagttga ctttgtgtcg cttatcggac tttttgcatc gccgtgtgcg gtttcatcat
     1621 tcacaatggc tcagcaaatg gacagcgact tcaaacttgc aggtgcggcg gttgtgttca
     1681 cttcggtttt agcgtgtttc acgatgtttt tgtggatttt cttcttcaga caggcaggtt
     1741 tcttttgata atctgcacga atttcagaca catgatttat tcaatgtgta aagttaaacg
     1801 atattattac tatcaataac aaaaaatagt ataacagtct tgacttttat tactgttagt
     1861 gttagaatac tcgcataggg taacggtttg caggaaatga cttgaaatcc tgcaaggtta
     1921 aactgttact ttattaaaca gctatttcct tcttttttta ttattttgtg ggcgtaagaa
     1981 aacggagtgc gtaatgcact ccgttttctt ttatacgaat tttctgaata taacaaaata
     2041 attgcaacac cgtagcatcc gcccatacat ttaaacgcaa cttctatata tacaaaaaca
     2101 gtcgggcgac a
//
LOCUS       DAOMVE010000208         2109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_15486_length_2109_cov_3.925024, whole genome shotgun sequence.
ACCESSION   DAOMVE010000208 DAOMVE010000000
VERSION     DAOMVE010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15486_length_2109_cov_3.925024"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..834)
                     /locus_tag="PLS64_08845"
     CDS             complement(100..834)
                     /locus_tag="PLS64_08845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL43962.1"
                     /translation="MYNNYIFDLYGTLIDINTDEWNDDLWKKIAILYAYKGAHYTYDE
                     LNEEYDRLVQAEKKAVLKKSPDTKVVDIKIEKVFRKLFTQKGIKVTNAEVLFIAEAFR
                     CYSTKYIKLYDGVLDLLDTLKAKGKKVYLLSNAQRSFTENELNMFDLTKYFDGICISS
                     DEECSKPDEKYFKTLFDRYGLEKSESIMIGNDYISDIGGAADFGIDSLYIHQSISPEI
                     DGELRSTYKVMDGDVFKIKKLIINHS"
     gene            984..1610
                     /gene="nth"
                     /locus_tag="PLS64_08850"
     CDS             984..1610
                     /gene="nth"
                     /locus_tag="PLS64_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRL43963.1"
                     /translation="MTKKEIAVNAVHALKKEYPDAICSLVYTDPLQLLIATRLAAQCT
                     DARVNMVTPSLFDRFKTAQDFADSTPEEVAEYIKSCGLYKTKSKDIVEMARMLCDDFG
                     GVVPDNIDDLTKLPGIGRKTANLVCGDIFGQPAVVVDTHCIRITKRLGLHDLKDQKKI
                     EFALRELLPPDESNDFCHRLVLHGRAVCTARKAKCEECCMNGFCPKKI"
     gene            1624..>2109
                     /locus_tag="PLS64_08855"
     CDS             1624..>2109
                     /locus_tag="PLS64_08855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRL43964.1"
                     /translation="MDKMKDIKLIALDLDGTTLADSDTLSKRTQTAIESAIKSGITVV
                     AASGRPFVMMPDSVMNIVGLDYAITSNGAVISKCGKTVHRSLILPDDVLKILGAVRND
                     DVILEGFIDGFTYADVRYVHRPLDYGCEPEYFEYTRSCHGKIVDMRSFLSKHRNELDL
                     IS"
BASE COUNT          594 a          424 c          452 g          639 t
ORIGIN      
        1 aactgtcttc ccgacattaa ttttccattt tccattttca attttacatt taataactct
       61 cgctcgggca ttaaatgtta tttattagtc aaactgtctt cacgaatgat taataataag
      121 tttcttgatt ttgaacacat cgccgtccat aaccttatat gtactgcgga gttcgccgtc
      181 aatctcgggg gaaatgctct ggtgaatgta caggctgtct atgccgaaat cagccgcacc
      241 gccgatatcg ctgatatagt cgttgccaat cataattgac tcgctctttt caagtccgta
      301 acggtcaaaa agtgttttga aatatttttc gtcaggctta gaacattctt cgtcagagct
      361 gatgcaaatg ccgtcaaaat attttgtcag atcaaacata ttcagctcat tttctgtgaa
      421 tgaacgctgt gcgtttgaga gaagataaac ctttttgcct tttgccttga gagtgtcgag
      481 aagatcaagt acaccgtcat aaagcttgat gtactttgtt gagtagcaac ggaatgcctc
      541 ggcaataaaa agtacctcgg catttgtaac cttaatgcct ttctgtgtga aaagcttacg
      601 gaaaaccttt tcaattttga tgtcaacaac ttttgtgtcg ggagactttt tgagaacagc
      661 ctttttttca gcctgaacaa gtctgtcgta ctcctcgtta agctcgtcat atgtgtagtg
      721 tgcacccttg taggcataga gaatggcaat tttcttccac aaatcatcgt tccattcatc
      781 tgtattaatg tcgataagag tgccgtataa gtcaaaaatg taattattgt acattttttg
      841 tcctccgaaa caaatttcta acttttatgt gtctattgta ccagtttttt atcctgattg
      901 caatagcaga atttttgtgt tatactgaag aaaacagtat ttaatcgcat tttttgcatt
      961 ttttaaaatt acggggtgaa tttatgacta aaaaagaaat tgccgtgaac gctgttcatg
     1021 ccttgaaaaa agaatatccc gacgcaatat gttcgcttgt ctacacagat ccgttgcagc
     1081 tgctcattgc aacaaggctt gccgctcaat gcacggacgc aagggttaat atggtcactc
     1141 cttcgttgtt tgacaggttc aaaacggctc aggattttgc cgactcaact cccgaagaag
     1201 ttgctgagta tataaaaagc tgcggacttt ataagaccaa atcaaaagat attgttgaaa
     1261 tggcaagaat gctttgtgac gattttggcg gtgtcgttcc cgataatatt gacgatttga
     1321 caaaactccc cggaatcggc agaaaaaccg caaatcttgt gtgcggtgac attttcggtc
     1381 agcctgcggt tgtggttgat acacattgca tacgcattac aaaaaggctc ggacttcacg
     1441 atttaaagga tcagaaaaag attgaatttg cattgcgtga gcttttgccg cccgatgaaa
     1501 gcaatgactt ttgtcaccga cttgttctgc acggcagagc agtctgcacc gcaagaaagg
     1561 caaaatgtga agaatgctgt atgaacggat tttgcccaaa gaaaatctga cagagggatt
     1621 tgtatggata agatgaaaga tataaagctg attgcacttg accttgacgg cacaactctt
     1681 gccgacagcg acaccttgag caaacgaacg caaactgcca ttgaatctgc gatcaaaagc
     1741 ggaataaccg ttgttgcggc aagcggcaga ccgttcgtta tgatgcccga cagcgtgatg
     1801 aacattgtcg gtctggacta tgcgattacc tcgaacggcg ctgtaataag caagtgcggt
     1861 aaaacggttc accgttcgct gattttaccc gatgatgtgt tgaaaattct cggtgctgtg
     1921 cgtaatgacg atgttattct tgagggattt attgacggat ttacatatgc cgatgtcaga
     1981 tatgtgcata gaccgcttga ctacggctgt gagcctgaat attttgaata cacccgttca
     2041 tgccacggca agattgtcga tatgagaagc tttttgagca agcatagaaa tgagcttgac
     2101 ctcatcagc
//
LOCUS       DAOMVE010000209         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_15548_length_2104_cov_3.715471, whole genome shotgun sequence.
ACCESSION   DAOMVE010000209 DAOMVE010000000
VERSION     DAOMVE010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15548_length_2104_cov_3.715471"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..966)
                     /locus_tag="PLS64_08860"
     CDS             complement(73..966)
                     /locus_tag="PLS64_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HRL43965.1"
                     /translation="MPDYNVFPDYKTRKEIINELCDRYKFIKHCFAGKSVCGRGIDVL
                     HIGNTKNRVLYCGGFHGSEYLTILALLKFFEECCEAMESDKTVGGCKIGNFLSIRGLT
                     IVPCVNPDGTEIALHGSDAAFKYKPLVEKVCTDTYKWQANARGVDINHNFNAGWCRLK
                     QLELKNNIKCPAPTRFGGNHPESEPETKALTTLCRNIDFERAIALHSQGREIYCSFGR
                     HTPVLSFRLASMFSQASGYNIAFPEEIATGGGFKDWFIEKFRRPALTIEMGLGKNPLP
                     LSDFEEEYKILQNILCLGAVV"
     gene            1018..1563
                     /locus_tag="PLS64_08865"
     CDS             1018..1563
                     /locus_tag="PLS64_08865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRL43966.1"
                     /translation="MELFEKTLDSKKIFDGRVLHIVLDEVELPDGKRSKREVVNHPGG
                     VCVAALDEDNNLSFVKQFRYPYKEVVLELPAGKLEKGSTPLENGKRELLEETGLEGYS
                     YISLGQVYPSPGYTSEIIHLYACRVKSQGEQKLDEGEFLNVEKIPLNKAVEMVLNNMI
                     PDSKTQIAVLKTAALLKSGKI"
     gene            1584..>2104
                     /locus_tag="PLS64_08870"
     CDS             1584..>2104
                     /locus_tag="PLS64_08870"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HRL43967.1"
                     /translation="MQNFTVSEHEKLNTAVALGFFDGLHKGHRRVILSAVEQKQNGLL
                     PVCFTFAQSPKEVLGKSSKGALMTTEDKLKTLESLGIEHTFSPDFKKLMNMSAKDFVE
                     KIIFGDLNAKFVVCGFNYRFGKNGEGDTELLKKLCDENGTKLKVIEPERDDGDVVSST
                     LIRLLISGGSIRRA"
BASE COUNT          573 a          444 c          496 g          591 t
ORIGIN      
        1 ttaatcaaag ttattaaaaa taacaaaata aacaagggca accgaggttg cccttgaatt
       61 atatcagata atttaaacta ccgctccgag acagagaatg ttttgcagga ttttgtattc
      121 ttcttcaaaa tcggagaggg ggagaggatt tttgccgagt cccatttcta tggtgagggc
      181 aggtcggcgg aatttttcga tgaaccaatc cttaaagccg ccgcctgtgg caatctcctc
      241 gggaaaggct atgttgtagc cgcttgcctg tgagaacatt gacgcaagcc gaaagctcag
      301 aacgggtgtg tgtctgccga atgagcagta aatttcccgt ccctgactgt gcagagcgat
      361 tgcccgttca aagtcaatat tacggcaaag tgttgtaagt gcctttgttt cgggttcgct
      421 ttcgggatga ttgccgccga atcttgtcgg cgccggacac ttgatattgt tctttaattc
      481 aagctgttta agtctgcacc aacccgcatt gaaattgtgg ttgatgtcaa ctccacgggc
      541 atttgcctgc catttatatg tatcggtgca gaccttttcg acaagcggtt tgtatttgaa
      601 tgcggcgtca ctgccgtgca aggcaatttc cgtgccgtcg ggatttacgc aaggcacaat
      661 cgtaagacca cgaatgctta aaaaattccc gattttgcat ccgccgacag ttttgtcgct
      721 ttccattgcc tcacaacatt cttcaaaaaa tttcaacagg gcaagtattg tgaggtactc
      781 cgagccgtga aaaccaccgc agtagagtac acgattcttg gtgttgccga tgtgcagaac
      841 atcaatacct cttccgcaca cgctttttcc ggcaaagcaa tgcttgatga acttgtacct
      901 gtcgcagagc tcgtttatga tttccttgcg tgttttgtaa tcgggaaaaa cattataatc
      961 gggcataaca aaatcctttc ctttcgataa ttcaatcttt aaaacggagg cataattatg
     1021 gagctttttg aaaaaactct tgacagcaag aaaatttttg acggcagagt tctgcatata
     1081 gtccttgacg aagtggagct tcctgacggt aaaaggtcaa agagggaggt tgtcaatcat
     1141 ccgggcggag tatgcgttgc cgctcttgat gaggacaata acctttcttt tgtaaagcag
     1201 tttcgctatc cgtacaagga ggttgtgctt gagcttcctg ccggcaagct tgaaaagggt
     1261 tctactccgc ttgaaaacgg aaagcgtgag cttcttgaag aaacgggact tgagggttac
     1321 tcctacattt ctctcggtca ggtctatccg tctcccggat acacctcgga aattatccat
     1381 ctttatgcct gtcgggtaaa gtcacagggt gagcaaaagc ttgacgaggg tgagtttttg
     1441 aatgttgaaa aaatccccct caacaaagcc gttgaaatgg tgcttaacaa tatgataccc
     1501 gactccaaaa cacagattgc cgttttaaag actgccgcac ttttgaaaag cggtaaaatt
     1561 taatttaatc ataggaaata tatatgcaga attttactgt ttcagaacac gaaaaattga
     1621 atactgccgt tgctctcgga ttttttgacg gacttcacaa gggacaccga agggttattc
     1681 tctcggctgt tgagcagaag caaaacggac ttttgcccgt gtgctttaca tttgctcaaa
     1741 gcccgaagga ggttctcgga aaatcttcta agggtgcgct tatgacaacg gaagataagc
     1801 tgaaaacgct tgaaagcctg ggtattgaac ataccttcag tcccgacttt aaaaagctga
     1861 tgaatatgtc tgcaaaggac tttgtcgaga aaattatttt tggcgacctc aatgcaaaat
     1921 ttgttgtctg cggttttaac tatcgtttcg gaaaaaacgg agaaggcgat accgagttgc
     1981 tcaaaaagct ttgtgacgag aacggtacaa agcttaaagt gattgagcct gagcgtgacg
     2041 atggcgatgt tgtttcatca acacttatcc gtctgcttat aagtgggggc agcattcgcc
     2101 gcgc
//
LOCUS       DAOMVE010000210         2047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_16132_length_2047_cov_2.438253, whole genome shotgun sequence.
ACCESSION   DAOMVE010000210 DAOMVE010000000
VERSION     DAOMVE010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16132_length_2047_cov_2.438253"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..777
                     /locus_tag="PLS64_08875"
     CDS             424..777
                     /locus_tag="PLS64_08875"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRL43968.1"
                     /translation="MGKQNYFFSGITDILILAILCEHDSYVYEIVKFIKEKSNGCLSI
                     SQNTVYAVTYKLVNEGKISEYSKLVGKKRTRIYYHIEDEGRAYYENLLESFHNAYSGV
                     ESILNILKEDKGNEQ"
     gene            complement(1117..1701)
                     /locus_tag="PLS64_08880"
     CDS             complement(1117..1701)
                     /locus_tag="PLS64_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43969.1"
                     /translation="MTENNKIDFETIDYSKVNPKKLNGNKRIVKSYLIYFAFDVVLTV
                     AIFLLTLFKLIDSSDNTVNTVIFILSGILCLCLYANNYYKSKDWRFYESLRSDTLTKL
                     IALFFVINGVLLLLTFNLAGVHISIVASWGGASLSILINNIVAYLDDLNKMYTTSKYD
                     TAIKFLKFWSVFILFAMFAYGYSSIGRYEYMTFR"
     gene            1795..1998
                     /locus_tag="PLS64_08885"
                     /pseudo
     CDS             1795..1998
                     /locus_tag="PLS64_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          703 a          337 c          321 g          686 t
ORIGIN      
        1 atttttacat agctgtaacg ctgtttatca tcacaataaa tttgtattct gttcaagcat
       61 taaaaaggag gctctttcga gcctccttac agcagttgtt ccaccctatt ttattgtaga
      121 gtttgttgct gtcaaaatgc aaatatcaaa actgccgatc tgttatttta cttttcatat
      181 ttgactatat caaaaagaat tcaaacaaaa tcaataatgt gtatggtgat atcatggctg
      241 atgagattaa tacacaaacg aattttttgg tcaattcgtt ttttacgaat actattgacg
      301 aaaaaatcac aatgtcggca acaaaaggct ttgctgtaat aactgctcat attaaattct
      361 tcttgaatgt attagattga tttttagtga ttaagtgtgt ataatgtata tcgggaggta
      421 tctatgggaa aacaaaatta ttttttcagc ggaattacgg acatactgat tcttgccatt
      481 ttgtgtgagc atgacagtta tgtgtatgaa attgtaaaat tcataaaaga aaaatcaaac
      541 ggatgcctgt ccatatctca gaacaccgtt tacgcagtca cctacaagct tgttaacgaa
      601 ggaaaaatca gcgaatattc aaagcttgtc ggcaaaaagc gcacacgaat ttattatcat
      661 attgaggacg aaggtcgggc atattatgaa aaccttttgg agagttttca taatgcttac
      721 agcggtgtag agagcatttt aaatatttta aaagaggaca agggcaatga acaataacgg
      781 ggattcccta tgttgtcaag aaaataagga caggcaaata catcaaaaga cttattgcgg
      841 ttgtacttat tcttgcactc atatcaactg ttacatactg cattgttttg catgaagacc
      901 atcagattac aaaaagacag gaagctgtta tatccgaaga tgtaatcgaa tagtataaat
      961 tccgttcgga tatttttaaa atttacagac cgatttcaaa tttgattttg aagtcggtct
     1021 ttttttgtat atacgctttt tacgcaatga aaatacatat tctgattgta ttgtatcaat
     1081 agacattaca aaaagcaata cttattaata ttttctttat ctaaaagtca tatattcata
     1141 tctgccaatg cttgaataac catatgcaaa catagcaaac aatataaata ctgaccaaaa
     1201 tttcaaaaat tttatagccg tatcatattt cgatgttgtg tacattttgt ttaagtcatc
     1261 taaataagca actatattat taattagaat agataatgat gcgccgcccc atgatgctac
     1321 tattgaaata tgtacacccg ctaaattaaa tgtcaaaagc agcaacacac catttatgac
     1381 aaagaatagt gcaatcagtt ttgtaagcgt atcagaacga aggctctcat agaatcgcca
     1441 atctttcgat ttataataat tattagcata aaggcacaaa cacaatattc cgcttaatat
     1501 aaatatcaca gtatttaccg tgttatcaga actatcaatc agtttaaata atgtgagcaa
     1561 gaatatagca actgtcagta caacatcaaa agcaaaatat atcaaatatg actttacaat
     1621 acgtttgttg ccgtttaact ttttcggatt gactttactg taatctatag tttcaaagtc
     1681 tattttattg ttttctgtca tgtcaaacac ctcatctcgc ccattctctt tttttcaatt
     1741 ggttggatac caatataatg actaatttta tcaatatcgg ttttgggatt atctgtccaa
     1801 agaatgaact caaccataat caaaagagaa tggcttaaca gatttcatat cattaactac
     1861 aaacatgcct ataggcttgt atttgagtat attgaaacct tttataatac cgttaggatt
     1921 cacagccact gcgactatat gtctcctaat gattttgaaa aattgtttga gagggtatct
     1981 tctctgccag cagcttagtt ggcagaactt ctcattttat tttgtgctaa atcttgacac
     2041 agaacca
//
LOCUS       DAOMVE010000211         1997 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_16643_length_1997_cov_3.084964, whole genome shotgun sequence.
ACCESSION   DAOMVE010000211 DAOMVE010000000
VERSION     DAOMVE010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16643_length_1997_cov_3.084964"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLS64_08890"
     CDS             complement(<1..421)
                     /locus_tag="PLS64_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRL43970.1"
                     /translation="MTDAKPPLQTYRTNRLVLPTDGKRESVKSINKSASSSGHTTEQK
                     PVYDFFKRIFDIICSSAALIILSPVFLILTVAIKTTDKGPVFFTHRRVGKDGKPLNIY
                     KFRSMVTNAEDLIKQFTPEQKAEYERNFKLEDDPRVTK"
     gene            complement(414..1127)
                     /locus_tag="PLS64_08895"
     CDS             complement(414..1127)
                     /locus_tag="PLS64_08895"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HRL43971.1"
                     /translation="MKKTKIDRSIDTEALGFQVRESYKTARTNIAYSIFKKGCKKVAF
                     TSSTKGEGKTVTSINVASALAQQVGTKVIVVECDLRRPRVNTALKITPTPGLTNYLNE
                     ECSLEDIVKDTKIENLKAITYGAIPPNPSELLASESMFDLIKTLEKNYDYIIFDTPPI
                     GVVIDALPIMKASDGVVIIARNNSTTYPELSRTVEVIKRTNAKILGVILNRVKPHETR
                     KGKSYYSRYGGYYKTSTND"
     gene            complement(1150..1872)
                     /locus_tag="PLS64_08900"
     CDS             complement(1150..1872)
                     /locus_tag="PLS64_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HRL43972.1"
                     /translation="MEITFKDITRIIKKNIVFIAVLSLLCAAASYFVTTFLVQKTYTS
                     TVKLYVETNYKSQSAYDDYQSINYAKNLVLTYIELLDSNSFYNSVSKELNEKYTASQL
                     KSMIKFESIEDTEVFKALVNSGSPSESKNIGNAIAKIAPDTIANVKDNAKLKIVDKAT
                     TPKAPTSPNVSRNVMIAFAAGLIISLIISFVRDFLDVKIKYNDEMTTVLDLPLLAAIP
                     DFEYFSNQKAAEKKYGDYESGY"
BASE COUNT          541 a          376 c          417 g          663 t
ORIGIN      
        1 cctttgtgac tctgggatcg tcttcaagct tgaagtttct ttcgtactca gctttttgct
       61 cgggagtaaa ctgcttgata agatcttcgg cattcgttac catacttctg aatttatata
      121 tgtttaaggg ttttccgtct tttcctacac gcctatgagt aaaaaataca ggacctttgt
      181 ctgttgtttt gatagctact gtaagaatta aaaagacagg cgacaatata atcagagcgg
      241 ctgacgagca tatgatgtca aaaatccgtt taaaaaaatc ataaacgggc ttttgttcag
      301 ttgtatgtcc gcttgatgac gcacttttgt ttatgctttt tacggattcc ctttttccat
      361 ccgtgggtag gacaagcctg ttagtgcggt atgtttgcaa aggcggtttg gcatcagtca
      421 tttgttgatg tcttgtaata tccgccgtat cttgagtaat agctcttgcc cttacgggtt
      481 tcgtgaggct ttacacggtt aagaataaca ccgagaattt ttgcattggt tcttttgata
      541 acttcaactg ttcttgaaag ctcgggatat gttgttgagt tatttcttgc aataataaca
      601 acaccgtcag acgccttcat aataggcagg gcgtcaataa caacgccgat tggcggtgta
      661 tcgaaaataa tgtagtcgta atttttttca agagttttga taagatcaaa catggattca
      721 ctcgcaagaa gctctgacgg gttcggcgga atagcgccgt atgtaattgc cttgagattc
      781 tcgatttttg tatcctttac aatatcctca agtgaacatt cctcgttgag gtagtttgta
      841 agaccgggag taggtgtgat ttttaaagct gtgttaactc tcggacggcg aaggtcacat
      901 tcaacaacga taacctttgt gccaacctgt tgagcaagag cagatgcaac attgattgaa
      961 gttacggttt tgccctcacc ttttgttgaa gaagtaaagg ctaccttttt acaacccttt
     1021 ttgaaaattg agtatgcgat gtttgttctt gctgttttgt atgattcacg aacctgaaag
     1081 ccaagtgcct cggtgtcgat tgaacggtcg attttagtct ttttcattgc agtttcactc
     1141 ctttaacaat taataacccg attcgtagtc accgtacttc ttttcggctg ccttctgatt
     1201 tgaaaaatat tcaaagtcgg gaatagcggc aagcaacgga aggtcaagaa cggttgtcat
     1261 ttcatcattg tacttgattt taacatcaag gaagtcacgg acgaatgaaa tgatgagtga
     1321 aataataaga cctgcggcga aagctatcat aacatttctc gaaacattcg gggatgtagg
     1381 agctttcggt gttgtagcct tgtcaacaat tttaagcttt gcattgtcct ttacattggc
     1441 aattgtatct ggtgcaattt ttgcaattgc gttgcctatg ttttttgatt cggacggact
     1501 gcccgaattt acaagcgcct taaaaacctc ggtatcttca atgctttcaa acttaatcat
     1561 tgatttaagc tgtgacgccg tgtatttttc gttgagttcc tttgaaacgg aattgtagaa
     1621 agaatttgaa tcgagcaact cgatataggt gagaacaagg ttctttgcgt agttgatact
     1681 ctgatagtcg tcataagccg actggctttt gtagttggtt tcaacataaa gctttacggt
     1741 tgatgtatat gttttctgaa ccaaaaatgt tgttacaaaa tatgacgcag ccgcacataa
     1801 aagcgacagc accgcaataa acacaatatt tttcttgata attctcgtta tgtccttgaa
     1861 tgttatctcc attaaattac tcctcttctc ggcttttgta cgcatatgac aagataatgc
     1921 cacagtcgac acgaaagtgc tgtaggcgaa aacggcacaa aagcacaaaa tacttttaaa
     1981 catgatactc ctattat
//
LOCUS       DAOMVE010000212         1993 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_16699_length_1993_cov_2.034572, whole genome shotgun sequence.
ACCESSION   DAOMVE010000212 DAOMVE010000000
VERSION     DAOMVE010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16699_length_1993_cov_2.034572"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1386)
                     /locus_tag="PLS64_08905"
     CDS             complement(<1..>1386)
                     /locus_tag="PLS64_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRL43973.1"
                     /translation="EFEKTSLGGGDIVSIITPEKDSLRCIVVDADPVLASFEKYFIGN
                     GLVTVGSEADNTVQCSFLNLISKHHCNLRFDGADTVLEDTSSNGTFVNHIRVGGRKVL
                     GFGEIIDVFGLKIICFGDMIAVGSLFSKFSVSDKIEKFTLPKVSYDTAHAVSNVEMFN
                     RSPRNFREICSETVVIEPPTVPQFSKKRSMLSVIGPSFTMAIPMVLGCGLTVFSTTLN
                     GSGANAFMFTGIITSVGSALMGGLWAYYNIKETKKREAEDESERFNIYGNYLIEIADY
                     IKEKYRQNFEALNTMYPSADICAEYTRNNPELWCRNFTHSDFLFCRLGTGDIDFQVDI
                     QIPKEKFSVTYDGLKDKPAMLYDNFKKLNNVPVGIDLQSKKLWGIAGGENKTGAFEIV
                     DDIIAQIAANISYTDVKIAFCFDEKEFEDKNRWDYVRWLPHVWSENKNTRYYATNRQE
                     CADVFFELANI"
     assembly_gap    1387..1396
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1397..1593)
                     /locus_tag="PLS64_08910"
     CDS             complement(<1397..1593)
                     /locus_tag="PLS64_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43974.1"
                     /translation="MILIIYRSQVCREIVLPNADNTDYRIFIDSKEFKLKKSFYLSLE
                     VIAHKWSITNENGEYKIVKNQT"
     gene            complement(1609..>1993)
                     /locus_tag="PLS64_08915"
     CDS             complement(1609..>1993)
                     /locus_tag="PLS64_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRL43975.1"
                     /translation="DINSDNKADAPNGQKIMIYIKTLNRKQKAQFVFDPEKGVNIGRD
                     SEKNNIFINEASVSMNHCKIFSDNMKVYICDLNSSNGTVLKRGLFGNYLLEGGSSCEL
                     MSGDRVCIGSNVFKITLFYYDMVTM"
BASE COUNT          592 a          428 c          333 g          630 t
ORIGIN      
        1 attatatttg caagttcaaa aaatacatcg gcacattcct gcctgtttgt cgcataatat
       61 ctcgtgtttt tattttccga ccatacatgc ggcagccaac gaacataatc ccacctgttt
      121 ttatcttcaa attctttttc gtcaaaacaa aatgcaattt tgacatccgt atagctgatg
      181 tttgccgcaa tctgtgcaat tatgtcgtca acaatttcaa aagctccggt tttattttca
      241 ccgcctgcaa taccccacaa tttttttgac tgcaaatcaa ttcccacggg aacattgttc
      301 agctttttaa agttgtcata aagcattgcc ggtttatcct ttaagccgtc atatgtaacg
      361 gaaaactttt ctttcggaat ttgaatatca acctgaaaat caatgtcacc cgttccgagt
      421 cggcaaaaca aaaaatcgct atgggtaaag tttctgcacc acagctccgg attgtttctt
      481 gtgtactctg cgcatatatc cgcagacgga tacattgtgt tcaatgcctc aaaattttgt
      541 ctgtattttt ccttaatgta atcggcaatt tcaataaggt aattaccgta aatgttaaat
      601 ctttcagatt cgtcctctgc ttcacgcttt tttgtttctt taatattgta atatgcccac
      661 aagccgccca taagagcaga accgaccgaa gtgatgatac ccgtaaacat aaatgcattt
      721 gcacccgaac cgttaagcgt agtgctgaac acggtaagtc cgcagccaag caccatagga
      781 attgccatgg taaatgacgg gcctataacg cttagcatag aacgcttttt tgaaaactgc
      841 ggaactgtag gcggttcaat aacaaccgtt tcgctgcata tctctctgaa atttctcggc
      901 gaacggttaa acatttccac atttgaaaca gcgtgagcag tatcatatga aactttcgga
      961 agggtgaatt tttcaatttt gtcggatacc gaaaattttg aaaacagcga gcccaccgca
     1021 atcatatctc cgaaacaaat tattttcaga ccgaaaacat ctattatttc tccgaaaccg
     1081 aggacttttc ttccgcctac cctgatatga tttacaaaag ttccgtttga gcttgtatct
     1141 tcaagaacag tgtcagctcc gtcaaatctg aggttgcaat ggtgttttga aatcaaattc
     1201 aaaaatgaac attgcacggt attgtcagcc tcactaccaa ccgtaacaag cccgtttccg
     1261 ataaaatact tttcaaaaga tgcaagcacg ggatcggcgt caacgacaat gcaacgaaga
     1321 gaatcctttt cgggagtgat aatagaaaca atatctcctc cgccgaggga tgttttttca
     1381 aattcannnn nnnnnngttt gattttttac aattttatac tcgccgtttt cgtttgttat
     1441 tgaccattta tgagcaatta cttcaagaga aagataaaaa ctttttttca gtttaaattc
     1501 cttgctgtca atgaaaatac gataatccgt attatctgca ttcgggagaa caatttctct
     1561 gcaaacctga ctgcggtaaa ttatcagtat cataacaaac ctccgttgct acatagtcac
     1621 catatcataa taaaacagcg taattttgaa tacattcgag cctatgcaga cacgatcacc
     1681 cgacataagc tcacatgaac ttccgccttc aagcaagtaa tttccgaaca gacctctttt
     1741 aagaactgtt ccgttagatg aattaaggtc gcatatatac actttcatat tatcggaaaa
     1801 gattttgcaa tggttcatag aaaccgatgc ttcattgata aaaatgttat ttttctcgga
     1861 atctctgccg atatttacac ctttttcggg atcaaataca aactgtgcct tttgtttacg
     1921 gttcaaagtt tttatgtata tcataatttt ctgaccgtta ggagcgtcag ctttgttatc
     1981 tgaatttata tca
//
LOCUS       DAOMVE010000213         1923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_17465_length_1923_cov_2.746253, whole genome shotgun sequence.
ACCESSION   DAOMVE010000213 DAOMVE010000000
VERSION     DAOMVE010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17465_length_1923_cov_2.746253"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLS64_08920"
     CDS             complement(<1..609)
                     /locus_tag="PLS64_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRL43976.1"
                     /translation="MKNKKIVPIIVSVIAMLSVICISSFTREKPPKKQNDINNIAALS
                     SKATADTPESDEEMRGVWLSYMELSMENESSKTQKAFEDKFTEIAQKCRESGFNTLIV
                     QVRPFCDALYKSSYFPWSHILTGTQGENPQYDALQIMCDICKENNLKIHAWINPYRVS
                     SNETPKKLSDNNPYIKNSEIGIKTDNGIFLDPSNETAQQLICD"
     gene            633..905
                     /locus_tag="PLS64_08925"
     CDS             633..905
                     /locus_tag="PLS64_08925"
                     /inference="COORDINATES: protein motif:HMM:NF018644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HRL43977.1"
                     /translation="MEIFIIYICIISLISIIVTVCDKYIAIHRKWRVRESTLLILSAL
                     GGSVAMYITMQLIRHKTRHIKFMLGIPIIFIIQLIILFFVWRMIYV"
     gene            898..1773
                     /locus_tag="PLS64_08930"
     CDS             898..1773
                     /locus_tag="PLS64_08930"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRL43978.1"
                     /translation="MSDRLYFSVPDECDNMLAQRFLRDKCGLSARIITRLKREKDGIL
                     MDGKILRTIDCVQSGKTVSIAIPDEKCEIEPTKGTLDIRYEDEHFLVVNKPHSMPVHP
                     VKQHQSDTLANIVSYYCSQNGKPFVFRAINRLDKDTSGLVLIAKNRFTANAVKNKTHK
                     VYYALCEGEINIEGIVDKPMDLRDDSKMVRIVRDDGMKAVTHYKPLVATKDFTLLELW
                     LETGRTHQIRCHMAYLGHPLMGDDLYGGKLDKISRQALHCARLEFYHPIKNEYITVTA
                     EIPEDMQSVINNKED"
BASE COUNT          594 a          356 c          371 g          602 t
ORIGIN      
        1 gtcacaaatc agctgttgtg cagtttcatt tgacggatca agaaaaattc cgttatcggt
       61 ttttatacct atttcggaat tctttatgta aggattgttg tctgaaagtt ttttcggagt
      121 ttcatttgaa ctgactctgt atggattaat ccacgcatga attttcaaat tgttttcttt
      181 gcatatatca cacataattt gcagtgcgtc atattgagga ttttcaccct gcgtacccgt
      241 gagaatatgt gaccacggaa aataacttga cttatataat gcgtcacaaa aggggcgtac
      301 ctgtacaata agcgtgttga aaccgctttc acggcatttt tgtgctattt cggtaaattt
      361 atcttcaaac gctttttgtg tttttgagct ttcattttcc atagaaagct ccatatatga
      421 taaccacaca ccacgcattt cttcatcact ttcgggtgtg tcggcagttg cctttgatga
      481 taaggctgca atgttgttga tatcattctg ctttttcggc ggtttttctc ttgtaaatga
      541 gcttatgcag ataacgctta acatagctat tacagataca ataatcggaa caatcttttt
      601 gtttttcaat caaatcatcc tttggagttc tcatggaaat tttcatcata tacatttgca
      661 tcatcagcct gatatcaatt attgtaacag tctgtgacaa atacattgca attcaccgca
      721 aatggagagt acgagaaagc acattgctga ttctctccgc actcggagga agtgttgcaa
      781 tgtacattac aatgcaatta atcagacaca aaacaagaca cattaaattt atgctcggca
      841 taccgataat atttattatt cagctgataa ttctgttttt tgtttggagg atgatttatg
      901 tctgacagac tgtatttttc cgtacctgac gaatgtgaca atatgcttgc tcaaagattt
      961 ctgcgtgata aatgcggtct ttctgcaaga ataataacac gactgaaaag agaaaaagac
     1021 ggtattttga tggacgggaa aattttaaga acaattgact gtgtgcaaag cggaaaaacc
     1081 gtttcaattg caattcccga tgaaaaatgt gaaattgaac cgacaaaggg gacacttgat
     1141 ataagatatg aggatgaaca ttttcttgtg gtaaacaaac cgcattccat gcctgttcat
     1201 cctgtaaagc agcaccaaag cgatacgctt gcaaatattg tttcgtacta ttgcagtcaa
     1261 aacggcaagc cgtttgtatt tcgggcaatt aatcgacttg acaaggatac ctcaggactt
     1321 gttctcattg ccaaaaaccg ttttacagcc aatgccgtaa aaaataaaac gcacaaagtc
     1381 tattacgctt tatgcgaggg cgaaataaat attgaaggta tagttgataa gccaatggat
     1441 ttgcgtgatg attccaaaat ggtgcgaatt gtccgtgatg acggaatgaa agcagttacg
     1501 cactacaaac cgcttgttgc aacaaaagat tttacattgc ttgaattgtg gcttgaaacc
     1561 ggcagaacac atcagattcg ctgccatatg gcttatttag ggcatccgct tatgggcgat
     1621 gacttgtacg gcggaaagct tgataaaatt tcaaggcagg cacttcattg tgcaaggctt
     1681 gaattttatc atccgataaa aaatgaatac attacggtta ctgccgaaat tcccgaagat
     1741 atgcagtctg taataaacaa caaagaggat tgaacaataa aaaggttcaa tcctctttaa
     1801 aacttacaaa cttttattga caatgtccgg acaaaaaaat ctatgtgcgt tcctcacact
     1861 tgcaatgaca gcaacattat ttgctggttg ttcagggaat tccgattcat aggattcaac
     1921 atc
//
LOCUS       DAOMVE010000214         1870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_18173_length_1870_cov_3.433058, whole genome shotgun sequence.
ACCESSION   DAOMVE010000214 DAOMVE010000000
VERSION     DAOMVE010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18173_length_1870_cov_3.433058"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1595
                     /gene="argH"
                     /locus_tag="PLS64_08935"
     CDS             219..1595
                     /gene="argH"
                     /locus_tag="PLS64_08935"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRL43979.1"
                     /translation="MQLWKGRFKKELSKTTNDFNSSISFDSRMYKEDIEGSIAHATML
                     GRCGIIKEEEANDIVKGLKGILADIENGTLHIDMEAEDIHTFIEGELTKRIGDNGKRL
                     HTSRSRNDQVAVDIKLYLKKEVVNVKNLVVGLIKVIADKAEKYSETVMPGYTHLQRAQ
                     PITFGHHLLAYGEMLLRDVSRLEDCLKRMDEMPLGSCALAGTTYPIDRTITAELLGFD
                     RITNNSLDGVSDRDYCIEFASVLSIIMVHLSRFSEEIIMWCSWEFKFIELDDAFSTGS
                     SIMPQKKNPDIAELVRGKSGRVFGDNMTLLTIMKGTALAYNKDMQEDKEAIFDALDTV
                     KMCLTAFTPMLDTMRVIPENMRKAAAGGFINATDCADWLTKNGVPFRDAYKATGELVA
                     RCIELGTDLENLPMDEYKKVCDVFNDDVYSAISLERCTNERTVFGGPASANVKAQAKR
                     VAEIAEKL"
BASE COUNT          585 a          394 c          410 g          481 t
ORIGIN      
        1 cgggggcagg gaatttctct gccccgataa gtgtttatta caccttttta ttttttacgg
       61 tatttgtcgg ttgaaaaata ttctgcgatt acttgcggtc gtctaatgaa cgcccccaca
      121 ggttaaccca acattaacaa caaggtgtaa ataaatattt aaaaccaaaa cgacaaagtg
      181 ctttacaacg gctaaacata atgtaaggag aaataataat gcagctttgg aaaggacgct
      241 tcaaaaagga gctttcaaaa accactaacg attttaacag ctcgatttca ttcgacagca
      301 gaatgtataa agaagacatc gagggcagca ttgcccacgc aacaatgctt ggcagatgcg
      361 gcattattaa agaagaagag gcaaacgata tcgtaaaagg tcttaaaggc attcttgctg
      421 atatcgaaaa cggtactctg cacattgata tggaggctga ggacattcac acctttatcg
      481 agggtgagct taccaaaaga atcggcgaca acggcaaaag acttcacact tcaagaagcc
      541 gtaacgatca ggttgcagtt gacatcaagc tttacctcaa aaaagaggtt gtcaatgtaa
      601 aaaacctcgt tgtaggtctt attaaggtta ttgccgacaa ggctgaaaag tacagcgaaa
      661 ccgttatgcc cggctatact cacctccaga gagcacagcc gattaccttc ggacatcatc
      721 tccttgctta cggtgaaatg cttttgagag atgtttcaag acttgaagac tgccttaaaa
      781 gaatggacga aatgccactc ggttcatgtg cgcttgcagg cacaacatat ccgattgaca
      841 gaacaatcac agccgaactc ctaggctttg acagaattac aaacaactct cttgacggtg
      901 tttccgacag agattactgc atcgaatttg catcggtgct ttcgataata atggttcacc
      961 tctcaagatt ttcagaggaa atcattatgt ggtgcagttg ggaatttaaa ttcattgaac
     1021 ttgatgacgc tttttcaaca ggatcatcaa ttatgccgca gaaaaagaac cccgatattg
     1081 cagagcttgt tcgtggtaag agcggcagag ttttcggtga caatatgacg ctcctcacaa
     1141 ttatgaaggg tactgcactt gcatacaaca aggatatgca ggaggacaag gaggctatct
     1201 ttgacgcact cgacacggtt aaaatgtgcc tcacagcctt tactcccatg ctcgacacaa
     1261 tgcgtgtcat tcccgaaaat atgagaaaag ccgcagcagg cggtttcatc aacgcaacag
     1321 actgtgccga ctggctcaca aagaacggtg ttccgttcag agatgcctac aaggcaacag
     1381 gcgaactcgt tgcaagatgt attgaactcg gtacagacct cgaaaatctt ccaatggacg
     1441 aatacaaaaa ggtttgcgat gtgttcaacg atgatgttta cagcgcaatt tcacttgaac
     1501 gctgtacaaa cgagcgtaca gtattcggcg gccctgcatc ggcaaatgta aaggctcagg
     1561 ctaagcgtgt tgcagaaatc gcagaaaaac tgtaattgca aatccgtagg ggcggatatt
     1621 atccgcccga cttgatgaaa ttttatgttt tatcaaaatg ttttaaagcg ataaaaagca
     1681 atgtatataa atatttgaac gctatgcgtt caatccgtat gcgattgcgt acggtcgggt
     1741 gtccgatgaa ctccactaca gataacagta aacttgttcc gcttaaatgg tttgttttta
     1801 ttataaaata tctgccatta cttgcgggcg gatgatatcc gcccctacaa acgggaacat
     1861 atgctttgtg
//
LOCUS       DAOMVE010000215         1790 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_19258_length_1790_cov_2.678963, whole genome shotgun sequence.
ACCESSION   DAOMVE010000215 DAOMVE010000000
VERSION     DAOMVE010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19258_length_1790_cov_2.678963"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(448..1653)
                     /locus_tag="PLS64_08940"
     CDS             complement(448..1653)
                     /locus_tag="PLS64_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HRL43980.1"
                     /translation="MRKIPFSPPDLSQAEIDEVIKVLKSGWITTGPETKLFESRIAER
                     CHTEKAVCLSSQTSCAELTLRILGIGPGDEVIVPAYTYTATASIIYHVGAKPVMVDCK
                     SGSFEMDYDKMEAAINHNTKVIIPVDLAGVVCDYDRIFEAVERKKGVFNPRNELQEKF
                     GRVIVMADGAHAFGAQWHGKMCGEIADFTNFSFHAVKNMTTAEGGAAVHRSHDGIDEE
                     EMYKQYMLLSLHGQTKDAYQKNKVASWEYDVVDTQYKCNMTDVLAAIGVAQLERYDQM
                     LERRHQLIKLYNEEFKDLPVQVLNHCDENHRSSGHLYFVRFIGKDAEYRNKFYNMMAD
                     HGVMCNVHFKPLPLLSAYSKRGFKITDYPNAYNMHKNQLTLPMNSTLTDDEAWYVIET
                     FKQCMKTLK"
BASE COUNT          491 a          416 c          358 g          525 t
ORIGIN      
        1 gcgaaccgtg ttcgccaatc gttttggttg caaaaccaaa acgaataaaa tgcccgtaca
       61 taatctttga tatatcaaac aaaataataa acaaatattt tgtattaggt cgaaccgatt
      121 ttacaatcgt ttaaaatggc gaacacggtt cgccgctaca ttatagcagt aactttgcaa
      181 catttatata attggattta acatagattt tctgccgtta cttagcgggc gtccaatgaa
      241 cgcccctaca gattccttac aaaacaaaaa tgcatcagag ccttaccggg catctgatgc
      301 attttattta tctgactgtc ttctcaaatt acgggcggtg gcacaccacc aaccttaatt
      361 ttcaactttc aatttcccat acgctcgggc gattttattc tcgcatttat aaaactgtct
      421 tcacgaaccg ggaacggcga ctcgccgtta tttaagtgtt ttcatacatt gcttgaatgt
      481 ttcgattaca taccatgcct catcgtctgt gagagttgag ttcatcggga gggtgagctg
      541 gtttttgtgc atattatacg cattcggata atcggtaatc ttgaaaccac gcttgctgta
      601 tgccgaaagc aacggcaacg gcttgaagtg gacattgcac attacgccgt ggtcagccat
      661 catattatag aacttgttac ggtattctgc gtctttgccg ataaatctta caaagtaaag
      721 gtgaccgctg ctgcggtggt tttcatcgca gtgattaagc acctgaacag gcaaatcctt
      781 aaattcttcg ttgtaaagct ttataagttg atgtctgcgc tcaagcattt gatcgtagcg
      841 ttcaagctga gccacaccga ttgcggcaag tacatcggtc atattacact tgtactgagt
      901 gtcaacaaca tcatattccc acgatgcgac tttgtttttt tgatatgcgt ccttggtctg
      961 accgtgaagt gagagaagca tatactgctt gtacatttct tcctcgtcaa ttccgtcatg
     1021 tgaacggtga acagccgcac cgccctcggc ggttgtcata ttctttacag cgtgaaagct
     1081 gaagtttgtg aagtcggcaa tttcaccgca cattttaccg tgccattgcg caccgaatgc
     1141 gtgagcgccg tcagccataa caataactct gccgaacttt tcctgaagct cgttgcgtgg
     1201 attgaaaacg cctttctttc tttcaacagc ctcaaaaata cggtcataat cgcacacaac
     1261 acctgcaaga tcaacaggaa tgataacctt tgtattgtgg ttgattgccg cctccatttt
     1321 atcgtagtcc atttcaaagc tgccgctttt gcagtcaacc ataacaggct ttgcgcccac
     1381 atggtagatg attgatgcgg ttgccgtgta ggtgtatgcc ggaacgataa cctcatctcc
     1441 cggaccgatg ccgagaatac gcagagtaag ttcggcacaa gaggtctgcg atgaaagaca
     1501 gacagccttt tccgtgtgac atctttctgc aatacggctt tcaaaaagct ttgtttcggg
     1561 acctgttgta atccagcccg atttcagaac ctttataact tcgtcaattt ctgcctgaga
     1621 aaggtcaggc ggcgaaaagg gaatttttct catttttaat ttctcctcag gtacagccgt
     1681 gcattaacca ctgccgtaca caaactatag acaaatctga aaatatttac actccattat
     1741 acaacgcaag tcataaaaag taaagttcca aaatacccaa aatatactct
//
LOCUS       DAOMVE010000216         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_19483_length_1775_cov_3.043023, whole genome shotgun sequence.
ACCESSION   DAOMVE010000216 DAOMVE010000000
VERSION     DAOMVE010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19483_length_1775_cov_3.043023"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            478..1467
                     /locus_tag="PLS64_08945"
     CDS             478..1467
                     /locus_tag="PLS64_08945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRL43981.1"
                     /translation="MKKLLCVFFCVMLAALSAVVFTGGKDTQKNYIKYVEFNVTKDAL
                     QNAVDLDIQTHDDDRHTDCITTLAYLGAKYGGDFTKYKYSDMTDFADKIKNGETVENL
                     TKDMKYFNYYSQAYGAALSGIVGDYEKETSNGTEKDYGLCWFSPIAKSFPYSCYDDFG
                     AVRTYGYTRPHLGHDLMAAVGTPVVAVESGTVEIMGWNRYGGWRIGIRSADKKRYWYY
                     AHLRQNRPFAENLKEGDKVCAGDVIGYVGRTGYSDTENTNGITESHLHLGLELVFDES
                     QKESNNEIWIDVGAITSVVEQNQSEVVRNNETKEFTRKYKFLVNNDLQTGATG"
BASE COUNT          583 a          318 c          367 g          507 t
ORIGIN      
        1 tgccctgcaa aattttcgat gggtgattct atcataactc atttcatgat ttatgataga
       61 ataagcacag caagtgtgat tagatttata attttgcaaa gtgcttatta tgtcaattta
      121 tattcccctc aaattttgct ctgcaaaatt ctcgatgggt gattctatca taactcattt
      181 cctgatttat gatagaataa actcacaaca taagaaaggg ttaacaaaca acccttattt
      241 acagttcgta aaggcagaac ggcttgattt ataacaagtc ttgcccgagc gtgacagaat
      301 aagtggtgat aacccatgtt aaacagctta caagattttc atttgatata tttttcctct
      361 ttctttaatt aaaaatatgg aagaaacagc gagttacacc gctgtttttt tcattttacg
      421 gcaaagccgc aatgaataac tgtatgccgt ttttcatata cattatcggt gattgatatg
      481 aaaaaactgc tctgtgtgtt tttttgcgta atgcttgcgg cattgtcggc tgtcgtgttt
      541 acgggcggaa aggacacgca aaaaaactac ataaaatatg ttgaatttaa tgttacgaaa
      601 gacgctttgc aaaatgccgt tgacctcgac atacaaactc acgatgacga caggcatacc
      661 gactgcataa ccacgcttgc gtatctcggt gcgaaatacg gcggtgattt tacaaagtac
      721 aaatacagcg atatgacaga ttttgccgac aaaatcaaaa acggcgaaac cgttgaaaac
      781 ctgacaaagg atatgaaata tttcaattac tactctcagg catacggtgc ggcactttcg
      841 ggaattgtcg gtgattacga aaaagaaact tcaaacggca ctgaaaaaga ctacggcttg
      901 tgctggtttt caccgattgc caaatctttt ccgtattcct gctatgatga tttcggtgcg
      961 gtcagaacct acggctacac aagaccgcac ctggggcatg atcttatggc ggctgtcggc
     1021 acacctgttg tggcggttga gtcgggaacg gttgaaatta tgggctggaa caggtacggc
     1081 ggttggcgaa tcggaatccg ttctgccgac aaaaaacgct attggtacta tgcacaccta
     1141 aggcagaacc gtccgtttgc agaaaactta aaagagggcg acaaggtttg tgcaggcgat
     1201 gtaatcggtt atgtgggaag aacgggttac agcgataccg aaaatacaaa cggaataacc
     1261 gaaagtcatt tgcatctcgg acttgagctt gtgtttgatg agagccaaaa agaaagcaat
     1321 aacgaaatat ggattgatgt gggtgctata acaagcgttg ttgagcaaaa tcaaagcgaa
     1381 gttgtgcgta ataacgaaac aaaggaattt acaaggaaat acaagttttt ggttaataat
     1441 gatttgcaaa caggtgcaac aggatagacg ctttggcaat tgttggcaat actgattctt
     1501 aacaaagtga ccaatgcaat ttttataata tcaaatgact ttactccaaa acacagactg
     1561 tcatacggca gtctgtgttt tgtctctaat tcgaaatttg tcacgaaatg tcaacaatac
     1621 cgaacagaac aatttattct gtcaaaagca caaaatcata aacattactt ttattcttta
     1681 caggcaaaaa atcatataat ttgtacgttg actgaaaaat agcaataagt taaaatatgt
     1741 aggacggtat ttgaaaaaat ttatgaattt aaatt
//
LOCUS       DAOMVE010000217         1753 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_19807_length_1753_cov_2.679034, whole genome shotgun sequence.
ACCESSION   DAOMVE010000217 DAOMVE010000000
VERSION     DAOMVE010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19807_length_1753_cov_2.679034"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..260
                     /locus_tag="PLS64_08950"
     tRNA            187..260
                     /locus_tag="PLS64_08950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:219..221,aa:Gly,seq:tcc)
     gene            complement(671..1246)
                     /locus_tag="PLS64_08955"
     CDS             complement(671..1246)
                     /locus_tag="PLS64_08955"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRL43982.1"
                     /translation="MSLFDKMKKYQPPEKPNDGEFKSDVSTILHGTSVQNRKEMCELE
                     YMKARRLLKNPTVETVARAKNIMNTLATDFDYLPAVLWMAEFSVNGVKNPEQAAMWYK
                     KAADLGSANGASKIADILMDGRGVTRNPKLAMEYYKIAADNGIASASFALGEYYAKTG
                     NREEAVKAYEKAVKGGYKDAEKKLAKLKKKF"
     gene            1534..>1753
                     /locus_tag="PLS64_08960"
     CDS             1534..>1753
                     /locus_tag="PLS64_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43983.1"
                     /translation="MNIFDNIAANLSLKYENSEFYKNKLIKRYKPLITPGVKSQDELI
                     ISLEKHFDLELTDKAVMSKTFLELCVMGN"
BASE COUNT          535 a          362 c          289 g          567 t
ORIGIN      
        1 gtcagccgca tcgaaaccaa agcgttaaaa caattgcgaa aacgatttga aacaagttcg
       61 aatttgtgag ataatatgtt atttaaattc atcaggggtg ttcatcgaat gcccctgtat
      121 atttcacaac aaaaaaattc ttgacatata ccaaactgcg tgatataata acaatgttac
      181 agataagcgg atgtagttta gtggtagaac ttcagccttc caagctgatc gtgtgggttc
      241 gattcccatc atccgctcca tactaaaagg tactcaatac tgagtacctt ttctttttgc
      301 atattgaaga aataaatcaa tcagatggga atcgaaagcc cgtttagaaa acagttcagc
      361 gaactgtttt tagggcgtag cgttgatcca agttaagcct aaacgcttta gacaaagacg
      421 agattggcaa cttctgtatc acaaagtaat catccgctcc atactaaaag gtactcgaaa
      481 atgagtacct tttctttttg cacatattac tgcgttcatg cgttcaaatt taaacacaac
      541 atcaatcaaa tcagaacatt tttataaagc ttacaatcta attgcaatgg gagaacacgg
      601 ttcttcccta caaaatgaca actactttgc ttcatttata tattatttga ctaaattaaa
      661 atcttaccaa tcaaaacttt ttcttcaatt tggcaagctt tttttcggca tctttatatc
      721 cgcctttgac ggctttttca taggctttta cggcttcttc cctattgcct gtttttgcat
      781 aatattcacc caatgcgaat gatgctgacg ctatgccgtt atcggcggca attttatagt
      841 actccattgc aagtttcgga ttgcgggtaa cacctctgcc gtccataaga atatctgcaa
      901 ttttggacgc tccattcgca ctgccaaggt ctgcggcttt tttgtaccac atagccgcct
      961 gttcgggatt tttgacaccg ttcacggaaa attcagccat ccacaatacg gcaggaagat
     1021 aatcgaaatc ggttgcaagg gtattcatta tatttttagc acgggcaacg gtttcaacgg
     1081 tcggattttt caaaagccgt cttgccttca tatattcaag ctcgcacatt tcttttctgt
     1141 tctgaacgct tgtaccgtgc agaatcgtgc ttacatcgct cttaaattca ccgtcattcg
     1201 gtttttcagg gggctgatat tttttcattt tatcaaacaa gctcataact ctcctcctta
     1261 ccgttttctg cttatcatat attttacttc aatattacga atacacaatt tttatcatat
     1321 caaaacattc agaaaaacat atgatagcat caaggctggc ggatgatatc cgcccctaca
     1381 agtttgtcat tctacattaa gattataaat gaaaactgcg aatttataaa tctgtttggg
     1441 cattatattc aaaaagcata ttgattttat ccttaaattc gattataatt tacttgtaaa
     1501 cataaaaacc acttgttttt taaggagata acgatgaata tatttgacaa cattgccgct
     1561 aatctttcat taaagtatga aaattcggaa ttttataaaa acaaactcat caaacgatac
     1621 aagccactca tcacaccggg tgtaaagtca caggacgagc ttatcatttc tcttgaaaag
     1681 cactttgacc ttgaactgac cgacaaggct gttatgagca agacttttct tgaactctgc
     1741 gttatgggca ata
//
LOCUS       DAOMVE010000218         1749 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_19870_length_1749_cov_3.068477, whole genome shotgun sequence.
ACCESSION   DAOMVE010000218 DAOMVE010000000
VERSION     DAOMVE010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19870_length_1749_cov_3.068477"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1749
                     /locus_tag="PLS64_08965"
     CDS             <1..>1749
                     /locus_tag="PLS64_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRL43984.1"
                     /translation="VLSFSKSSNTASSTVCENINAKLDSLSKEVDGLHFTNLYNEGDY
                     INLVINSVLQNLLMGAVLAIIILFLFLRDIKPTLIVACSIPISVVFAIVLMYFSGVTL
                     NMISLSGLAIGVGMLVDNSVVVIENIYRLRSMGVSPIKAALNGARQVAGAITASTLTT
                     VCVFLPIVFVEGITRQLFVDLALTVTYSLLASLIVALTLVPAMGQRMLRKMKPNSAPK
                     DGKIKKAYERSLRFVLKHKVPAILVAVALLFTSAGLCLMRGFSFMPDMSSTQIQVSLK
                     LDDNATFEETVKEGEKLNDLLSKYDQFETVGVMAGNSSSLMGLTGGSSSNDAGSLMAY
                     AVLKDDFTKQSGEISKKIEKDLESLDGEAAVSGGTSSSMSSLMGDGSVQITLYGDDLD
                     TLKSTAEDIGKTLEKVKGVASVDNGIGAVSPEIKVTVDKSKAAEKGLTVAQVYQQVAA
                     AISTEKTAATLTNDDGNDMNVVVVKDDSETVTPKNLRDIDITYKDSSGNEKTVKLSSV
                     SDISKSETMDKISRENQKRYLTISAEVKDGYTLTSVSNNVKSAMNDYKLPKSCSIDYA
                     GTDKMTMEAVSQLMLML"
BASE COUNT          499 a          385 c          393 g          472 t
ORIGIN      
        1 tgtattgagc ttttcaaagt caagcaacac agcgtcttct accgtttgcg aaaatattaa
       61 cgcaaagctt gattcgcttt caaaagaggt tgacggactt cactttacaa acctttataa
      121 tgaaggcgac tacattaatc ttgtaatcaa cagcgttttg cagaacctcc ttatgggtgc
      181 ggttcttgca attatcatac ttttcctatt tctcagagat atcaaaccga cgctcattgt
      241 tgcctgctca attccgataa gcgttgtgtt tgcaattgtc ctcatgtatt tcagcggtgt
      301 aacactcaat atgatttcgc tgtcaggact tgcgatagga gtcggtatgc ttgttgacaa
      361 ctcggttgtt gttatcgaaa atatctaccg actgagaagt atgggcgttt cgccgattaa
      421 ggcggcactt aacggtgcaa gacaggttgc cggcgcaatt acggcatcaa ctctcaccac
      481 agtatgcgtg ttcctgccga ttgtatttgt tgaaggcatc acccgtcagc tgtttgttga
      541 ccttgcactc acggttacat attcacttct tgcaagcctt attgttgcgc tgacgcttgt
      601 tccggccatg ggacagagaa tgctccgcaa gatgaaacct aattccgctc cgaaggacgg
      661 aaaaatcaag aaggcatatg aacgctcact tcgtttcgta ttaaagcaca aagttcctgc
      721 aattctcgtt gcagttgcac ttctctttac aagtgcaggt ctttgtctga tgagaggttt
      781 cagctttatg cccgatatga gcagtacaca gattcaggta tcattgaagc ttgacgataa
      841 cgcaaccttt gaagaaacag ttaaagaggg cgaaaagctc aacgatttgc tttcaaaata
      901 tgaccagttt gaaaccgtag gcgttatggc gggcaactcg tcaagcctta tgggacttac
      961 aggcggttca tcgtcaaatg atgccggctc acttatggca tatgccgttc tcaaggatga
     1021 ttttacaaaa cagagcggtg agatttcaaa gaagattgaa aaggatcttg aaagccttga
     1081 cggtgaggct gccgtaagcg gcggcacttc aagttcaatg tcaagcctta tgggtgacgg
     1141 ttccgttcag attacgctct acggtgacga ccttgacaca ctcaaaagca ctgccgagga
     1201 tatcggcaag acacttgaaa aggttaaagg cgttgcatcg gttgacaacg gcatcggtgc
     1261 tgtttctccc gaaatcaagg ttactgttga caagtcaaaa gccgctgaaa agggacttac
     1321 cgttgcacag gtttatcagc aggttgccgc tgcaatttca acagaaaaaa ccgctgccac
     1381 tcttacgaat gatgacggca atgatatgaa tgttgtggtt gtaaaggacg acagcgaaac
     1441 cgttactccg aaaaatctcc gtgacattga cattacctac aaggattcaa gcggtaacga
     1501 aaagacagtt aaactctcct ctgtttcgga tatttcaaag tcggaaacta tggataaaat
     1561 cagccgtgaa aatcaaaagc gttatcttac aatcagcgcc gaagtcaagg acggctacac
     1621 acttacatct gtttcaaaca atgtaaaatc cgcaatgaac gattacaagc tcccgaaaag
     1681 ctgttcaatc gactatgcag gtacagacaa aatgacaatg gaggcagttt cacagcttat
     1741 gcttatgct
//
LOCUS       DAOMVE010000219         1712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_20492_length_1712_cov_2.073627, whole genome shotgun sequence.
ACCESSION   DAOMVE010000219 DAOMVE010000000
VERSION     DAOMVE010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20492_length_1712_cov_2.073627"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLS64_08970"
     CDS             complement(<1..203)
                     /locus_tag="PLS64_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43985.1"
                     /translation="MKFTVPKLIFLLATIAIIAIILSGCNIDDVLGYLSTVRVDEYDM
                     EVNYYNEGATYLFSGKVVLTKYD"
     gene            complement(221..>1712)
                     /locus_tag="PLS64_08975"
     CDS             complement(221..>1712)
                     /locus_tag="PLS64_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRL43986.1"
                     /translation="YSQSYGSDSVLSYDYSYDANGNITEIKQNGKLTNKYVYDSLNEL
                     KEEYDYVNKFYINYSYDRAGNLQNKYEQVLDPTYGYPTGTQNGNTYEYTDTSWKDKLT
                     KVNGSNISYDANGNPLTYRDGMSFEWENGRVLKKINTSDKSVQMSYDSSGMRTQKTVD
                     GVKTNYYYDSNKNLIALVKGNDTLLFYYDSDGSATSFSYNGTMYFYVKNLQGDVIRII
                     DLAGTEVASYVYDAWGNIKDTKGEPTIREINPIRYRGYVYDTETSLYYLQSRYYDPFT
                     GRFLNADDTHYIMFPSLMANLYTYCDNNAISNTDTNGKTAWLWMLGLYAVKKSMPLQF
                     YFHSILNRQRYKCYDFSDNSMYADKILGSMAYYNFKKKYHIINLYPALERMYHQNRLY
                     MIYSGTFDPYHTGERDLYLSIGQCKVNICYVIYGNVNKRHDIAYSVTVTFTDDYDFEH
                     WTKSKKNLLTMCINNILGYYPQSLGLIVPYSWRIQYKFSGTFRCRH"
BASE COUNT          500 a          340 c          293 g          579 t
ORIGIN      
        1 tcatcatatt tggtcagcac taccttgccg gaaaagagat atgttgcgcc ctcattatag
       61 taatttacct ccatatcata ttcatcaacc ctgacggttg acaaatatcc caacacatcg
      121 tcaatattgc atcccgacaa aataatagca ataatagcaa tagtagctaa caaaaatatc
      181 agcttcggta cggtaaattt catcttagcc tccttttgca tcaatgtcga catctgaacg
      241 tgcctgaaaa tttatactgt atacgccatg agtagggaac tatcaatccg agcgattgag
      301 gataatatcc taaaatgtta tttatacaca tggtcaataa attcttttta cttttagtcc
      361 aatgttcaaa atcgtagtca tcagtaaaag ttactgtcac actataagca atgtcatgcc
      421 ttttgttaac attaccgtat attacatagc agatatttac tttacattga cctatcgata
      481 aatataagtc tcgttcaccc gtatgataag gatcaaaagt tccactataa atcatatata
      541 atcgattttg gtgatacatt ctttctaatg ccggatataa atttatgatg tgatatttct
      601 tcttaaaatt ataatatgcc atcgaaccca aaattttatc ggcatacata ctattatccg
      661 aaaaatcgta acacttgtag cgttgcctat taagtattga atgaaaatag aattgtaatg
      721 gcattgactt cttcactgcg tacaagccta acatccataa ccaagctgtt ttaccatttg
      781 tatccgtatt actaattgca ttattatcac aatatgtata caaatttgcc atcaaggacg
      841 ggaacataat gtaatgtgta tcgtccgcat tcaaaaatct gcctgtgaat ggatcgtagt
      901 agcgactttg gagataatag agtgaagttt cggtatcata aacatagcca cggtagcgaa
      961 tcgggtttat ttctcgaatt gtcggctcgc ctttggtatc ttttatgtta ccccaagcgt
     1021 cataaacata gcttgcgact tctgtacctg cgaggtcaat aattctgatt acatcaccct
     1081 gcaagttctt cacataaaag tacattgtac cgttgtatga gaatgaggtt gcacttccgt
     1141 cactgtcata gtagaacagc agagtatcat tgccctttac aagtgcaatt aggtttttgt
     1201 tgctgtcata gtaatagttg gtcttaactc cgtcaacggt tttctgcgtt ctcataccgc
     1261 ttgagtcata gctcatttga acggatttgt cggaagtatt gatttttttg agaactcttc
     1321 cgttctccca ttcaaagctc attccgtcac ggtatgtcag agggttgccg tttgcatcgt
     1381 atgaaatatt actgccgtta acctttgtca gcttatcctt ccagcttgta tcggtgtact
     1441 cataagtatt gccgttctgc gttcctgtcg gataaccgta ggtgggatca agaacctgtt
     1501 catatttatt ttgcagattt cctgctctgt cataggaata gttaatgtaa aatttattta
     1561 catagtcgta ttcttcttta agctcgttaa ggctgtcata aacatacttg tttgtaagct
     1621 taccgttttg cttaatctcg gtaatattac cgtttgcatc ataagaataa tcgtaggata
     1681 aaacgctgtc actgccgtat gactgactgt ac
//
LOCUS       DAOMVE010000220         1703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_20632_length_1703_cov_3.223301, whole genome shotgun sequence.
ACCESSION   DAOMVE010000220 DAOMVE010000000
VERSION     DAOMVE010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20632_length_1703_cov_3.223301"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..700)
                     /locus_tag="PLS64_08980"
     CDS             complement(119..700)
                     /locus_tag="PLS64_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRL43987.1"
                     /translation="MDANVTDLRIVKTRTNIKNSLIDLLAEKNVSKITVTELAEKAMI
                     NRKTFYRHYHTVQDVVDDINYDIVNDVISHAKKSDRDGNNLVNQLNFIGLSIVENKEQ
                     INKILKNSPEVFGSGRSVELLKRFIEVSIRPVLNFKNETKLKYLVEFVVSGFISVYVR
                     WFNDGCAESSEKLADAVNEFVLGALSEYVRPKS"
     gene            765..1604
                     /locus_tag="PLS64_08985"
     CDS             765..1604
                     /locus_tag="PLS64_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HRL43988.1"
                     /translation="MTGLIIEGGARRGIFTAGITDCLLENKIHFDYVAGVSAGAEVAL
                     DFTAKQEGRSRRVIMPHRTGDFSVLGFLSMDLDNMIYRFPYQDYPFDFDTFFASDTNC
                     EFVATDCVTGKAKYFSENKSEQRLLDSVRASCTVPILYQISEFEGGKYVDGSIADAVP
                     FDRAFEQGCSRVLVLLTKPENEPCTDYRKFEFLINKKYKDYPNLINALMTRFDRYNEQ
                     CKRMKQLEEDGILMVLRPSHKYVKSFEMNTDVLDEAYNAGKNLAKEKLAEIEDFLNVL
                     EKK"
BASE COUNT          511 a          355 c          350 g          487 t
ORIGIN      
        1 cagggaattt tgcaaaaacc gctgtctttg cgatgaaaca atgaaaaaaa tttcaaagcc
       61 tattctctcc cccaaaggat tcgaggcaat tactaaggct cattggaaaa tgagttaatc
      121 agcttttcgg gcgaacatat tcggacaatg ctccgagaac gaactcgttg acagcgtctg
      181 caagcttttc ggagctttct gcacagccgt cattgaacca acggacataa accgagatga
      241 atcccgaaac gacaaactca acaaggtatt tgagttttgt ttcattttta aaattaagca
      301 caggtcttat tgaaacctcg ataaatcttt tcaaaagctc aacagagcgt cccgagccga
      361 acacctcggg agaatttttc agaattttat taatctgttc tttattttca acaatggaaa
      421 gaccgataaa attcagctga ttcacaagat tgttgccgtc acggtcggat tttttcgcat
      481 ggctgataac atcgttgact atatcgtaat tgatatcatc gacaacatcc tgaaccgtgt
      541 gataatggcg gtaaaaggtt ttgcggttaa tcatcgcctt ttctgcaagc tcagtcaccg
      601 tgattttgga aacatttttt tcggcaagca aatcaatcag cgaatttttt atattggttc
      661 ttgtttttac aatgcgaagg tcagtcacat tagcgtccat actatcaccc cataaaacag
      721 tttgattata gcaccaaaat ttttttgagt caacggaggt ccttatgaca ggtctgataa
      781 ttgaaggcgg agcaaggcga ggaattttta ccgccggtat aaccgactgt ctgcttgaaa
      841 acaaaataca ttttgactat gttgcaggcg tttcggcggg agctgaggtc gcccttgatt
      901 ttaccgcaaa acaggaaggt cgttcccgca gggttattat gccccacaga acaggcgatt
      961 tttcggttct cggttttctt tcaatggatc ttgacaatat gatttacaga tttccttatc
     1021 aggactatcc gttcgatttt gatacatttt ttgcgtcaga tacaaactgc gaatttgtgg
     1081 caaccgactg cgtaacaggc aaggcaaaat atttttccga gaacaaaagc gaacagcgat
     1141 tgcttgactc cgtaagagcg tcctgcacag ttcctattct ctatcagata agcgaatttg
     1201 agggcggaaa gtatgttgac ggcagtattg ccgacgctgt tccctttgac cgtgcgtttg
     1261 aacagggctg ttcccgtgtg cttgtgcttt tgacaaagcc cgaaaatgag ccttgcacgg
     1321 actatcgcaa gtttgaattt ttaatcaaca aaaaatacaa ggactatccg aacctcatca
     1381 acgctcttat gacgagattt gaccgctata atgagcaatg caaaagaatg aaacagcttg
     1441 aggaggacgg catacttatg gtactccgcc cgtcacacaa gtatgtaaaa tcatttgaga
     1501 tgaacacgga tgtgcttgac gaggcgtata acgccggcaa aaatcttgcc aaagaaaagc
     1561 ttgccgaaat tgaagatttc ctcaatgttc tcgaaaagaa ataatcaaaa caacaagtta
     1621 cattaatata tcttcccaaa aagcaaggcg caatttcgtg ccttgctttc tttttatatg
     1681 caatttcaac aagtaaaaag tgt
//
LOCUS       DAOMVE010000221         1670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_21164_length_1670_cov_3.114551, whole genome shotgun sequence.
ACCESSION   DAOMVE010000221 DAOMVE010000000
VERSION     DAOMVE010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21164_length_1670_cov_3.114551"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..>1670)
                     /locus_tag="PLS64_08990"
     CDS             complement(240..>1670)
                     /locus_tag="PLS64_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRL43989.1"
                     /translation="SAYTAPKEGKIFYNKTMYDKYGKAEGMVLDSLKNFDEEIDISSL
                     NVPRSDVAEFFKVLTLTHPELYYVNQGFSYSYYPSEDKVTSIYPEYTISKSEYATQKK
                     SLDKEVERILSLVDENMTDSEKALVIHDELAIMSEYSTSDYNKADIYNSLVEKTSVCQ
                     GYALAYSYMLSLVGIDSELVDSNSMNHMWNKVHIGNAWYNVDVTWDDPINDRPGHAQH
                     TYFLLSDNAIQNLPSKHYDYTISYGANSTKYDNYEIHNFDTRLCEVNGEFYGFVNNNS
                     SANKGALLKYKLDSNTYEKKLKLNERWSAGGNSYWINTFMSLEKYNDTLYFNTSDAVY
                     SYDVETGRQAVAASDISGVSASSGKLCYGMVLKGDAMYVAVSDSPNNKATLQYVKRVD
                     KGNLAIGMTNYVMTASTNSAEKAYYGDANGDGKINISDATAIQKAIVGIVNQNEIERI
                     NSDVNFDSDITVIDATTIQMYIVGLI"
BASE COUNT          455 a          357 c          325 g          533 t
ORIGIN      
        1 tagaattata gcataacaat tcctctaaat caatacttaa agtatgtgcg gatattatac
       61 gcctgtagat agccacgcaa agatttgtac aaattaaaat tatataaata aagcaaaaat
      121 tgcatcaatg ccgtatcggg cattggtgca attcctttat ctaactatct tccggaaagg
      181 actgcggtgt gctaccgcag tcctaaattt tccattttca atttgccaac ggcttgccgt
      241 tatataagcc ctacaatata catctgaatt gtggttgcgt caatgacggt aatatcggaa
      301 tcaaagttta catcagagtt tatacgctct atctcgttct gattgacgat acccacaatt
      361 gctttctgaa ttgcggttgc atcggaaata tttattttgc cgtcaccgtt tgcgtcaccg
      421 taatatgcct tttcggctga attggtgctt gcagtcataa catagtttgt cataccgatt
      481 gcaaggttgc ccttgtcaac tctttttaca tactgcaaag ttgccttgtt gttcggagaa
      541 tccgacacgg caacatacat tgcgtcaccc ttgagaacca taccgtagca aagcttgcct
      601 gatgatgcgg acacaccgct gatgtcggat gcggcaactg cctgtctgcc tgtttcaaca
      661 tcgtatgaat acacggcgtc ggaggtatta aaatacagag tatcgttgta tttttcaagg
      721 ctcataaatg tatttatcca gtaggaatta ccgcctgcgc tccatctttc gttcaatttc
      781 agctttttct catatgtatt gctgtcaagc ttgtatttta acaatgcacc cttgttagct
      841 gaagaattgt tgttgacaaa gccgtaaaat tcaccgttaa cctcgcaaag ccttgtgtcg
      901 aaattatgga tttcgtaatt atcatatttt gtcgaatttg caccgtagct tattgtgtaa
      961 tcatagtgct ttgacggaag attctgaatg gcattgtcac tcaaaaggaa gtatgtatgc
     1021 tgagcgtgtc ccggtcggtc attaatagga tcatcccatg taacatcaac attataccaa
     1081 gcgttgccga tatgaacctt gttccacata tggttcattg agtttgaatc aacaagttcg
     1141 gagtctattc cgacaagtga aagcatgtag gaatacgcaa gtgcatagcc ctgacatacg
     1201 gaagtttttt caacaagcga attgtaaata tcagccttgt tgtaatctga tgtgctgtac
     1261 tcgctcataa tggcaagctc atcgtgaatt acgagtgcct tttcggaatc ggtcatattc
     1321 tcgtccacaa gcgaaagaat tctctcaacc tctttatcaa gcgacttttt ctgtgttgca
     1381 tactcgctct tgctgattgt gtactcggga tagattgacg taaccttatc ctctgacgga
     1441 tagtaggagt acgaaaatcc ctgattcaca tagtaaagct ccggatgagt caaggtcagc
     1501 accttaaaaa attccgcaac atcgcttctc ggaacattta gactgctgat atcaatttcc
     1561 tcgtcaaaat ttttaaggct gtcaagaacc ataccttcag ccttaccgta tttgtcgtac
     1621 attgttttgt tataaaaaat tttgccctct ttcggcgctg tgtatgccga
//
LOCUS       DAOMVE010000222         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_21504_length_1649_cov_3.212045, whole genome shotgun sequence.
ACCESSION   DAOMVE010000222 DAOMVE010000000
VERSION     DAOMVE010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21504_length_1649_cov_3.212045"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..926)
                     /gene="ispE"
                     /locus_tag="PLS64_08995"
     CDS             complement(57..926)
                     /gene="ispE"
                     /locus_tag="PLS64_08995"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HRL43990.1"
                     /translation="MEVKVLAPAKINLSLDVLSKRADGYHNISTVMQAVDLYDTITIT
                     DNESGTVTISCNCEGVPCDDSNICAKAAYKFFDYCKTDVKGIHIDIDKKIPTQAGLAG
                     GSSDGAAVILGLNNMFGTMLKPAEMREIGEKVGADVPFCLEGGTKLCTGIGTAIKKVA
                     SFRCDSIVICKPDTVSVSTADAYKKVDALNPHACYTDEMVKALFSRDMFMISTTIFND
                     FELALQIPEVNEIKGIMLKSKARGAGMSGSGSAVFGVFTSSRRAKKCYDKLCESYKEV
                     FLCKPVSDGCKVL"
     gene            complement(930..>1649)
                     /locus_tag="PLS64_09000"
     CDS             complement(930..>1649)
                     /locus_tag="PLS64_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRL43991.1"
                     /translation="KASDLRAAITPKTKLLVLPFPNNPTGAIMEREDLEEIAEVIKEH
                     NIFILSDEIYCELTYGNKNHVSIAEIDGMYERTIVVNGFSKSYAMTGWRLGYALGPVP
                     VIAQMTKLHQYGIMSAPTTAQYAAIEALRNGDRDVEMMRGEYDMRRRLVVDSFNAMGL
                     TCFEPLGAFYVFPCIKSTGLTSEEFCTRLIVDKHVAVVPGTAFGESGEGFVRVSYSYS
                     IKHLKIALERIKEFLDELKKG"
BASE COUNT          455 a          388 c          326 g          480 t
ORIGIN      
        1 tcttgaaata agtatggaca ggttattgta attttaatca gaattattaa attttatcaa
       61 agcaccttgc aaccgtctga aacgggcttg cagagaaaaa cttctttgta gctttcacaa
      121 agcttgtcat agcacttctt ggctctgcgt gatgaagtga acacaccgaa cacggctgaa
      181 cccgaaccgc tcatacctgc tccccttgcc tttgacttga gcataatacc cttgatttca
      241 ttaacttccg gaatttgcag agcaagttca aagtcattga agattgttgt tgaaatcata
      301 aacatatctc tgctgaacag tgcctttacc atttcatcgg tatagcaggc gtgaggattg
      361 agtgcgtcaa cctttttgta tgcatcggca gtagacacgc tgactgtgtc gggtttgcag
      421 attacaatgc tgtcacaacg aaaagaggca acttttttga ttgcagtgcc tattcccgtg
      481 caaagctttg ttccgccctc aaggcagaaa ggcacatccg cacctacttt ttcaccgatt
      541 tctctcatct ctgccggctt gagcattgtt ccgaacatat tgttcaagcc taaaataaca
      601 gccgcaccgt cactgcttcc gccggcaaga cctgcctgtg tgggaatttt cttgtcaatg
      661 tcgatatgaa tgcccttaac atccgttttg caataatcaa agaacttgta agccgccttt
      721 gcacaaatgt ttgaatcgtc gcaaggcacg ccctcacagt tacatgaaat tgtcaccgtg
      781 ccgctttcat tgtctgtgat tgtgattgta tcatacaaat caacggcttg cataacagtt
      841 gaaatattgt gataaccgtc tgctctcttt gaaagaacat cgagagaaag atttattttt
      901 gcaggagcaa gaacttttac ttccatattt taaccctttt tcagttcatc aagaaactct
      961 ttgattcttt caagcgcaat tttaagatgc ttgatagaat atgaatatga cacacggaca
     1021 aagccttcgc ccgattcacc gaacgcagta cccggaacaa cggcaacatg cttgtccaca
     1081 ataagtcttg tacagaattc ctcacttgta agacctgtgc ttttgataca cgggaacaca
     1141 tagaatgcac ccaaaggttc aaagcaggtg agtcccattg cgttaaagct gtccacaaca
     1201 agccttctcc tcatatcata ctcgccacgc atcatctcta catccctgtc accgttgcgc
     1261 agagcctcaa tagcggcata ctgtgcggtt gtaggagcag acataattcc gtactggtgg
     1321 agctttgtca tctgagcaat aacaggaaca ggaccgagtg cataaccaag cctccagcct
     1381 gtcatggcat atgattttga aaagccgttt acaacaatgg tgcgctcata cataccgtcg
     1441 atttcagcaa ttgaaacatg gttcttattg ccgtatgtaa gctcacaata aatttcgtcc
     1501 gaaaggataa aaatgttatg ttccttaatt acctctgcaa tttcttcaag atcttccctt
     1561 tccataattg caccagttgg gttgttcggg aacggaagta caagaagctt tgtctttgga
     1621 gtaattgcgg cacgaaggtc tgacgcttt
//
LOCUS       DAOMVE010000223         1631 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_21833_length_1631_cov_1.732868, whole genome shotgun sequence.
ACCESSION   DAOMVE010000223 DAOMVE010000000
VERSION     DAOMVE010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21833_length_1631_cov_1.732868"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1631)
                     /locus_tag="PLS64_09005"
     CDS             complement(<1..>1631)
                     /locus_tag="PLS64_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43992.1"
                     /translation="IDSMMRNFSRHRAEETKEGYNWTQWQAEQLKSLEEYRKHNAKKF
                     GKRFKTINGKVEEMIRTAKADGNASQEAEILEAVKDGFKAPKKPSAHSTAEFFKVNDR
                     KLDALIKSTTDDLKRAETAVLRMSNDKYRKAIFNAQVAMNTGAVTYEKAVDIACKDML
                     NAGLNCVEYKNGARHTLSDYADMAVKTANKRAYLRGEGEKRAEWGVSLVVVNSRQGGC
                     PDCAKYIGKVFIDDVYSNGKKSDGNYPLLSTAIKNGLFHPRCKDSTSTYYPELDDLDA
                     PLSEDEIKELDRQRGIEEKQQYAQRQAERFDRRAEYSLDEDNKRIAQTRADEWHDRAN
                     TLEEKAKQFSLNTNEQKYYRPVFEEDISKTFERKIEGETITIDTHKANTLCDNVYISD
                     KVKLKRKELHNFDMQVRKAFDMLGEVETSGKPEICIVTPEEMRVNAIASYMPMQNVLN
                     VNSAYFSTSDLSGLQENLACPQDGLSTILHELIHWQDAKNYRAKFGSINDYFEYCDYL
                     NKIYAPKVEKLINNGYNIEDISEYAFECLKDKAMD"
BASE COUNT          375 a          375 c          310 g          571 t
ORIGIN      
        1 tcatccatag ctttatcttt taagcattca aaagcatact cacttatatc ctctatatta
       61 taaccgttat ttatcaattt ttcaaccttt ggggcataaa ttttattaag gtaatcgcaa
      121 tattcaaaat aatcgttaat acttccgaat tttgctctgt aatttttagc gtcttgccaa
      181 tgaatcagtt cgtgcagaat tgtactcaat ccgtcttgcg gacaagccaa gttttcttgt
      241 aagcctgaca aatcacttgt tgaaaagtat gctgaattga catttagaac attttgcatt
      301 ggcatatatg aagcaatagc atttactcgc atttcttcgg gagtgacaat acaaatttca
      361 ggctttccgc ttgtttcaac ctctccgagc atatcaaacg cttttctcac ttgcatatca
      421 aaattatgaa gttcttttcg ttttagcttt accttatctg aaatataaac attatcacac
      481 aatgtatttg ccttgtgggt atcaattgta attgtttcgc cctcaatttt gcgttcaaaa
      541 gtttttgata tatcttcttc aaaaacaggt ctgtaatatt tctgttcatt ggtgtttagt
      601 gagaattgtt ttgccttttc ttcaagcgta ttcgccctat cgtgccactc atcggctcgg
      661 gtttgggcaa tgcgtttatt gtcctcgtca agactgtatt cggcacggcg gtcaaagcgt
      721 tctgcctgtc gctgtgcata ctgctgtttt tcctcaattc ctcgctgacg gtcaagctct
      781 ttgatttcat cttcagacaa cggtgcgtcc aaatcatcaa gttcgggata atatgtactt
      841 gtgctgtcct tacatctcgg atgaaacaaa ccgttcttga ttgcggttga gaggagcgga
      901 tagtttccgt ctgacttttt gccgtttgaa taaacatcgt caataaacac cttgccgata
      961 tattttgcac aatcggggca accgccctgt cttgagttca caacaacaag ggatactccc
     1021 cattcggctc gcttttcgcc ctcgccacga agataggctc ttttgttggc tgttttaacc
     1081 gccatatccg cataatcaga gagcgtgtgc cttgcaccgt tcttgtattc cacacaatta
     1141 agacctgcgt tgagcatatc tttgcaagct atatcaacgg ctttttcgta tgtaaccgca
     1201 cccgtgttca ttgcaacctg tgcgttaaaa atcgccttgc ggtacttgtc gttgctcata
     1261 cgcaaaactg ccgtttctgc cctctttaaa tcgtctgtgg tcgattttat gagtgcgtca
     1321 agtttacggt cattcacctt aaaaaactcg gctgtgctgt gtgctgacgg ctttttcggg
     1381 gctttgaaac cgtccttgac agcttcaaga atttctgcct cctgacttgc atttccgtca
     1441 gctttggcgg tgcgaatcat ctcttcaacc ttgccgttaa tggttttgaa acgcttgcca
     1501 aatttctttg cgttgtgctt acggtactct tcaagacttt tgagctgttc agcctgccat
     1561 tgtgtccagt tgtaaccctc tttggtttct tcggctctgt gacggctgaa attgcgcatc
     1621 atgctgtcaa t
//
LOCUS       DAOMVE010000224         1612 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_22198_length_1612_cov_1.696211, whole genome shotgun sequence.
ACCESSION   DAOMVE010000224 DAOMVE010000000
VERSION     DAOMVE010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22198_length_1612_cov_1.696211"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(205..690)
                     /locus_tag="PLS64_09010"
     CDS             complement(205..690)
                     /locus_tag="PLS64_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HRL43993.1"
                     /translation="MIVYAQGDITKFDVDAIVNASNGFGYMGGKRCVNELHRGVAESI
                     QFASCGAVERLARAKCKAHSFFGYSPGTVFVTDAPNLECKKIIHAVTMRTPGSKAKIK
                     KIKVLIPKIVAVAENLNINSVAIPLLGCGTGGLDRKTVQNLLAHNLISDKVTFYIVTS
                     L"
     gene            complement(687..>1612)
                     /locus_tag="PLS64_09015"
     CDS             complement(687..>1612)
                     /locus_tag="PLS64_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43994.1"
                     /translation="QLLETKQKFISQVMTDKAPARKCADVDQAALSYGEIKALCTGDD
                     RIREKLTLDNRVKELNSLKADYNNTKYELEDAVAAYPEKRKQLLCTIGNIKSDIDNVK
                     SFVDKDGKLICKLTLGSQTFNLKNDEERKNAAKALTTAVAQVRIADNRKKQVSVGNIC
                     GFDISVINNGNVYDDIVVTIKGKYSYSAQLGVSAVHNLSKIEGLLYKTESVLDYSQNS
                     LNKLDLDYKTSVALLEKPFEYEAELQEKSARLSELTKILNIELAHQKTESKPKTHYFG
                     KDKILGRNKSSKQVQNKTVMKSEPKRSEPEL"
BASE COUNT          463 a          320 c          288 g          541 t
ORIGIN      
        1 attcctcaat aaaagggagc aactgcatat aatgcagttg ctccaaaaat cgttactttt
       61 tgtcgcattc tcctgatatg gtggtcagac ggtagcgaag cacaacacta agatgagtaa
      121 ccaacatcgt tcaaatacta ttatatgtta taactaagaa aaaacaagtg ttttaaaaag
      181 aaattcagaa aagaatttct gattttaaag tgaagtaaca atataaaaag tcactttgtc
      241 acttataaga ttatgagcta ataaattttg aacggttttt ctgtcaagtc cacctgttcc
      301 acacccaagt aaaggtatgg ctactgaatt tatgtttaag ttttcagcta ccgctactat
      361 ctttggaatc agaactttaa tttttttgat ttttgctttg ctacccggag tacgcatagt
      421 aactgcgtgt attatttttt tacactcaag atttggagca tcagtcacaa aaacagttcc
      481 gggtgaataa ccaaaaaagg agtgtgcctt acattttgct cttgcaagcc gttcaacagc
      541 accgcaagat gcaaattgaa tgctctctgc aactcctcta tgtaattcat taacacatct
      601 tttacctccc atataaccaa aaccgttgga tgcatttaca atagcatcta catcaaattt
      661 tgttatatcg ccttgtgcgt aaactatcat aattcaggtt cactcctttt aggctctgat
      721 ttcataactg ttttattctg aacttgcttt gaagatttgt ttctgccgag gattttatcc
      781 ttaccaaagt aatgtgtttt gggctttgat tctgtttttt ggtgagcaag ctcaatgttc
      841 aaaatctttg ttaattcaga caggcgtgct gacttttcct gcaattccgc ttcatattca
      901 aaaggctttt caagcagggc aacacttgtt ttgtaatcaa ggtcaagctt gttaagagaa
      961 ttctgactgt aatcaagaac actttcggtt ttgtacagca acccctctat tttactgagg
     1021 ttatgcacag cagaaacacc gagctgagca gaatatgaat atttaccctt gattgtaact
     1081 acaatgtcat catacacatt tccgttgttt ataacgctta tatcgaatcc gcaaatgttg
     1141 ccaacgctaa cctgcttctt tctgttatcg gctattctta cctgagcaac agcagttgta
     1201 agagctttgg cagcattttt tcgttcctca tcgttcttca ggttaaaagt ctgagaacct
     1261 aaagttaatt tgcaaataag tttaccgtcc ttatcgacaa atgacttaac attatcgata
     1321 tcggatttta tgttgccgat agtacaaagg agttgcttgc gtttttcggg ataagccgca
     1381 acagcatctt caagctcata ttttgtattg ttgtaatcag ctttgagaga gttaagctcc
     1441 tttactctgt tatcaagcgt gagcttctca cggatacggt catcgccggt acagagagcc
     1501 ttaatttcgc catatgaaag agccgcttgg tcaacatcgg cacacttgcg tgcaggagct
     1561 ttgtcagtca taacctgact tataaatttt tgcttggttt cgaggagctg at
//
LOCUS       DAOMVE010000225         1608 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_22265_length_1608_cov_3.595621, whole genome shotgun sequence.
ACCESSION   DAOMVE010000225 DAOMVE010000000
VERSION     DAOMVE010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22265_length_1608_cov_3.595621"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="PLS64_09020"
     CDS             complement(<1..146)
                     /locus_tag="PLS64_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRL43995.1"
                     /translation="MFCDLHTHSTFSDGTFSPTEIVLKAKEIGLDAVALCDHNTVTGL
                     DEFVS"
     gene            complement(236..1240)
                     /locus_tag="PLS64_09025"
     CDS             complement(236..1240)
                     /locus_tag="PLS64_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRL43996.1"
                     /translation="MDIKEYEEFCKNSKYGNFMQSADWAKVKKGWIPEYVAVRDEYGK
                     INGCTLVLVKKIPILNTAMLYAPRGFVCDTHDSKTVRKIFEQIKLIAEKYHAYTLKTD
                     PLIDETDFISIKNLVDLGFVYHGEKQGYDNTQCRENYVLDIKDKSCDEVFANFKSKWR
                     YNIRLAMRKGVECKFCGEDELDDFMELMKETGRRDGFEIRSEEYFRNFLQAFHGNAKL
                     CITKLDGKVLSGALLVNYANVASYVYGCSSNEYRKYMPNYLMQWTMIKYAIESGCHTY
                     DFCGIPYWYDETHKNYGVYRFKQGFNGKVKTYAGEFDYVFRNGVNHIADLALKLKKLV
                     "
     gene            complement(1324..>1608)
                     /locus_tag="PLS64_09030"
     CDS             complement(1324..>1608)
                     /locus_tag="PLS64_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43997.1"
                     /translation="MLFDTKTFTIYELNIDTTYLSDSGTESENPLYSVIPYSDSYEQE
                     LSDALIKYWGVSSDSITVNVSPESFSINICPQPFLEYSKINNEQFDYYVL"
BASE COUNT          453 a          338 c          295 g          522 t
ORIGIN      
        1 gaaacaaatt cgtcaagtcc tgtaaccgtg ttgtggtcgc agagtgcaac agcgtcaagc
       61 ccgatttctt tggcttttaa aacgatttcc gtaggtgaaa atgtgccgtc cgaaaatgtt
      121 gaatgtgtat gcaaatcgca gaacataaaa ttcctccgat acaaaatgga gtgggataat
      181 tttacccact ccattttagc atatacggtt tgcaaattca acgcagatga atatttcaca
      241 caagcttttt cagtttcagt gcaaggtcgg caatgtgatt tactccgttt ctgaaaacat
      301 agtcaaactc gccggcatag gttttgactt tgccgttaaa tccctgttta aagcgatata
      361 caccgtagtt tttatgcgtt tcgtcatacc aatagggaat accgcaaaaa tcgtatgtat
      421 ggcaaccgct ctcaattgcg tacttaatca tcgtccattg cataagataa ttcggcatat
      481 attttctgta ttcgtttgat gaacagccgt aaacatagct tgcaacattt gcataattta
      541 caagcaatgc acccgacaaa acttttccgt caagctttgt gatacagagc tttgcgttgc
      601 cgtgaaatgc ctgtaaaaaa tttctgaaat attcttccga acgaatttca aagccgtcac
      661 gcctgcctgt ttctttcata agctccataa aatcgtcaag ctcatcttca ccgcagaatt
      721 tacactcaac gcctttcctc attgcaaggc ggatgttgta ccgccatttt gatttaaaat
      781 ttgcaaaaac ttcatcacac gatttatctt ttatgtcaag cacataattt tctctgcatt
      841 gggtgttgtc atatccttgc ttttcaccgt gataaacaaa gccgaggtca acaagatttt
      901 taatcgagat aaagtcggtt tcgtcaatca gcggatcggt tttgagtgtg tatgcgtgat
      961 atttttctgc aatcagcttt atctgctcaa atatttttct gacggtttta ctgtcgtgag
     1021 tgtcgcatac aaagcccctc ggtgcataaa gcattgccgt gttgagaatc ggtattttct
     1081 ttacaagcac gagtgtacag ccgttgattt ttccgtattc gtcacgaact gcaacatact
     1141 cgggaatcca acccttttta acctttgccc agtctgccga ttgcataaag tttccgtatt
     1201 tactgttttt acaaaattcc tcatactctt taatatccat aaaaaaacaa gcctttcgta
     1261 agtgtatcta tactatacaa aaggcttgtt tctgaaattg ttcaaaaatg tatcatagtt
     1321 gtttcataac acataataat caaattgttc gttgtttatt ttgctgtatt ccaaaaacgg
     1381 ctgtgggcag atgttgatgc tgaaagattc gggacttaca ttaaccgtta ttgaatcgga
     1441 actgacaccc cagtatttta tgagtgcatc ggacaactcc tgttcataac tgtcgctgta
     1501 cggaataacc gaatacagcg gattttcact ctcggtaccg ctgtccgaca ggtaggttgt
     1561 atcaatgtta agctcataaa ttgtaaaggt tttcgtgtca aacagcat
//
LOCUS       DAOMVE010000226         1586 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_22701_length_1586_cov_2.073155, whole genome shotgun sequence.
ACCESSION   DAOMVE010000226 DAOMVE010000000
VERSION     DAOMVE010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22701_length_1586_cov_2.073155"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1081)
                     /locus_tag="PLS64_09035"
     CDS             complement(203..1081)
                     /locus_tag="PLS64_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL43998.1"
                     /translation="MPKLEIKGITKVYKKGAVKALDNFSVTLTPGVYGLLGPNGAGKS
                     TLMNIITDNLNADGGEVVYGGENIKKLGKDYRAVLGYMPQQQGLYDDFTLNRFLWYMA
                     ALKGLKKKEAKEKITQLLETVNLTDAAHKKLGSFSGGMKQRALIAQALLNNPEILILD
                     EPTAGLDPKERIRIRNFISEIAEDKIVLISTHVVSDIEFIAKEIILLKKGQLISHDSP
                     NKLTKELENKVFEVEIQREDLKYFQDNYRVSNLCHNDNSIIVRIVTDNPPTDCQYTTA
                     KPTLEDLYLYIFEQGM"
     gene            complement(1068..>1586)
                     /locus_tag="PLS64_09040"
     CDS             complement(1068..>1586)
                     /locus_tag="PLS64_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL43999.1"
                     /translation="NLIRTTENGKTKLFFGKIVVECIYLLIAFTALYVPYFVRFINTY
                     GANSLNTPLVCIFENVQETSFSVINAVVVNLICYFLLATAVTFVITAVSIFTRSSMFT
                     MVVSTVLVILPLLALYSIENVRIGYWVVNSHIIAIVMTCLLSILIAIVTLEISKLKFT
                     ETRIWRRINAKA"
BASE COUNT          471 a          323 c          288 g          504 t
ORIGIN      
        1 gaacaaacca tattgataca agctgtgttg taaagccttt tcatagcaac ataaatattg
       61 caaagcctat ccacactatc ggcatatgag catttttgta tttacattcc gttataccat
      121 tccgatttta tgtaaaaagt gcataaaaat accgacaagt attagcatag aacgccatta
      181 cttgtcggca ataattttaa tatcacattc cttgttcaaa aatatagagg tacaaatctt
      241 caagagttgg ttttgcggtt gtgtattggc agtctgtcgg cgggttatcg gtcacgatgc
      301 ggactattat gctgttgtcg ttgtgacaga gatttgaaac acggtaattg tcttgaaaat
      361 attttaaatc ttccctttga atttcgacct caaagacttt attttcaagc tcctttgtca
      421 acttattcgg ggaatcgtgg gagatgagct gacctttttt aagcaaaata atctcctttg
      481 cgataaactc aatatccgac acaacatggg tggagattag gacaatttta tcctcggcaa
      541 tctcgctgat gaaattgcgt atgcgtattc tctccttcgg gtcaagtcct gccgttggct
      601 cgtcaagtat gagaatttcg ggattgttca gaagcgcttg agcaatcaag gctctctgct
      661 tcataccgcc cgaaaacgag cctaactttt tatgtgcggc atctgtcaga ttaaccgttt
      721 caagaagctg agtgatcttt tccttggctt ctttcttttt taagcccttt aatgccgcca
      781 tataccacaa aaagcggttg agggtgaaat catcatacaa gccctgctgt tggggcatat
      841 agccgagtac ggctcggtaa tccttgccga gctttttgat attttcaccg ccgtaaacca
      901 cctctccgcc gtcggcattg aggttgtcgg tgataatgtt catcagcgtg gacttgcctg
      961 ctccgttcgg tccgagcaag ccgtatactc ccggagttaa ggtaacgctg aaattatcaa
     1021 gcgcctttac cgctcctttt ttatagactt ttgtaatacc tttaatctca agctttggca
     1081 tttattcgcc tccatattct tgtttcggta aattttaatt tagaaatttc aagtgtgaca
     1141 attgcaatta agatactcag caagcaagtc ataacgatag ctataatatg ggaatttaca
     1201 acccaataac ctattcttac attttcaatc gaatacaaag caagcaatgg taatataact
     1261 aaaacagttg aaaccaccat tgtaaacata ctgctacggg taaaaattga aactgctgta
     1321 ataacaaatg ttactgctgt tgcaagcaaa aagtaacaaa tgagattgac aactaccgca
     1381 tttatcacac taaaagatgt ttcttgaaca ttttcaaaaa tacatactaa gggtgtattc
     1441 aaactgttcg caccgtatgt atttataaat cttacaaaat acggcacata cagagctgta
     1501 aatgctatta acaaatatat acactccact actatttttc caaaaaacag ttttgtttta
     1561 ccgttttcag tagtacgaat aaggtt
//
LOCUS       DAOMVE010000227         1520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_23943_length_1520_cov_3.316724, whole genome shotgun sequence.
ACCESSION   DAOMVE010000227 DAOMVE010000000
VERSION     DAOMVE010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23943_length_1520_cov_3.316724"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..762)
                     /locus_tag="PLS64_09045"
     CDS             complement(91..762)
                     /locus_tag="PLS64_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRL44000.1"
                     /translation="MEILKVENLTKTYGSGENLVHAVDDVSFSVEKGEFVAIVGASGS
                     GKSTLLHLIGGVDRPTSGKIFVDGNDISKMNDDKLAVFRRRQVGIVYQFYNLIPILTV
                     EENITLPCDLDGRGVDRERLEMILDSFGLRARRKHLPNQLSGGQQQRTSIARALINNP
                     SLVLADEPTGNLDSKSSEEVMSMLKMCNQSYGQTVIMITHNLDIAKQADRIITISDGK
                     IVEEG"
     assembly_gap    969..978
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          426 a          327 c          297 g          460 t
ORIGIN      
        1 ccgagagcat aattcccacg attgtaacaa ttgtacgcac cttgttaagc cgcaggtttt
       61 taagagtaag cttgttgaga agtttcataa tcaaccctcc tcaacgattt taccgtcact
      121 tattgtgata attctgtcag cctgttttgc aatgtcgagg ttgtgcgtaa tcattataac
      181 agtctgtccg taactctgat tgcacatttt gagcatactc ataacctctt cgcttgactt
      241 tgaatcaagg ttgcctgtcg gttcatcggc aagcacaaga gacggattgt taatgagcgc
      301 tctggcaata gatgttcttt gttgctgacc gcccgaaagc tggtttggaa ggtgttttct
      361 tcttgcacgc agtccgaacg aatcaagaat catctcaagt ctttctctgt caacacctct
      421 gccgtcaagg tcacagggga gcgtgatgtt ttcttcaaca gtcagaatcg ggataagatt
      481 gtagaactga tagacaatgc ccacctgtct gcggcggaat acggcaagct tgtcatcatt
      541 cattttgctt atatcgttgc cgtcaacaaa aatttttccg cttgtgggac ggtcaacacc
      601 gccgataaga tgaagaagag ttgacttgcc gctgccgctt gcaccgacaa tggcaacaaa
      661 ttcgcccttt tcaaccgaga acgaaacatc gtcaaccgca tggacaaggt tttcaccgct
      721 tccgtaggtt tttgtaagat tttcaacttt cagaatttcc ataaaatata cctccaaatt
      781 cattttacac ccatattata gtgacgcaaa atgactgtaa agtgaatcct gcaaacaaat
      841 tgttttaata tctaaaacat tggaatacac aactttcttc acatcaaaac atacgaggat
      901 aaacataaat tttcatcttg aatacaagta aaacatttga tataattgtt cggtaaggaa
      961 atggcggcnn nnnnnnnnca caaaataatg tagcggcaaa ccgtgttcgc caatcgtatc
     1021 gtgtgcaaac acgaaaggat taactatcaa tttttctgtt catctgtagg ggcgttcatc
     1081 ggacgcccgc tttgatggaa tgttatgttt atacacatat gttttgatat gatgaaagta
     1141 gtgcaataac tttcatataa acaaaaaatg catcaatgct gtatcgggca tcaatgcaat
     1201 ttctttatct gactatcttc tcgaattgag tgcggtggct taccgccgac cgcaattttt
     1261 cattttcaat tttcaacttt caatttaaaa gtaacgctcg ggcgataatg cttgaactat
     1321 cgcaaactgt cttcacgaat tgggcgtagc gacacgctac ctgcagtttt gaaaatatat
     1381 tttgatgaga ttattcggct tgtcggcaat gcctatgcct aaaaaatcac catcatgggt
     1441 tttcattctg aaaagctgac cgttttcgta ccccgacttg gcaaggtatg ttctgtcggc
     1501 acttaacgca ccgccgtttg
//
LOCUS       DAOMVE010000228         1487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_24660_length_1487_cov_1.977654, whole genome shotgun sequence.
ACCESSION   DAOMVE010000228 DAOMVE010000000
VERSION     DAOMVE010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24660_length_1487_cov_1.977654"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    232..241
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<242..1427)
                     /locus_tag="PLS64_09050"
     CDS             complement(<242..1427)
                     /locus_tag="PLS64_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRL44001.1"
                     /translation="MKKLLSLKTLLICISFTVFLLLVKDNMNVVWSAIGWITGIITPF
                     AIGFLFAYILNYPYKFFYSKVFGRMGRKRKFLANFRKPLALIITYAIVVAIFVVIISI
                     VVPQIAASLFSLINSLPQYAGVLSDMTKTVVHWINHTFSMEIDASEVINNFFKYITNF
                     ISAGNITSFLGNSTNAVFSAIMSTCNGVYNFLLGIVISVYFLAAKETLCRQIKKLAVA
                     FIPIKYLPKIYEIVDITDTKCGRFLVGDIIDAAFLGVLTFITMSICQLPYAPLIAVLI
                     GVTNIIPFFGPFIGAIPSAFILLLIDPIYMIIFIIIIVIIQQLDGNLFKPKIIGSQVG
                     LSSFWVLFSVIVGGGMFGVLGLILGTPIYAVIYALVGKRVKNKIDSKGRIAQEALDFE
                     VLN"
BASE COUNT          469 a          289 c          284 g          435 t
ORIGIN      
        1 attatctgag gattcgtctt catcatttgt tttgttacgg ttgatgaatt tcataaattt
       61 ttctctttgc tgcatttctt tttcttcacg aagttttttc tgctctgcgg caattttggt
      121 gtagttaagc acctcgaaat caagtgcttc ctgagcaatt ttgccctttg attcaattct
      181 gtttttaact cttttacctc atgttgttgc atgtaatact ctaaatcttc annnnnnnnn
      241 nagttaagca cctcaaagtc aagagcctcc tgagctattc ttccctttga gtcaattttg
      301 tttttaactc tcttgccgac aagagcatag attacggcat agattggtgt gccgagaata
      361 agaccgagca caccgaacat tcctccgccg acaattacgc tgaacagtac ccagaagctt
      421 gaaagaccga cctgactgcc gataatcttc ggcttgaaaa gattgccgtc aagctgctga
      481 attataacta taatgattat gaaaataatc atgtaaatcg gatcaataag caagaggata
      541 aaagcactcg gaattgcacc gatgaacggt ccaaagaacg gaataatatt cgtcacaccg
      601 ataagaaccg caataagcgg tgcgtacggt aattgacaaa ttgacattgt aatgaatgtg
      661 agaacaccga gaaaagccgc atcaatgata tcgccaacga gaaaacgacc gcattttgta
      721 tctgtaatat cgacaatttc ataaatctta ggaagatact ttatcggaat aaaagcaaca
      781 gcaagctttt ttatctgacg gcaaagtgtt tcctttgcgg caaggaagta aactgaaata
      841 acgattccga gcaggaagtt atacacaccg ttacacgtag acataattgc gctgaatacg
      901 gcatttgtag agttgccgag aaaactggtg atattgcctg ccgaaataaa gtttgtaata
      961 tacttgaaga agttgtttat tacttcactt gcgtcaattt ccatactgaa tgtatggtta
     1021 atccaatgca ccactgtttt tgtcatgtca gaaagtacac ctgcatactg cggcaggctg
     1081 ttaataagac tgaacaggct ggctgcaatc tgcggaacta caattgagat tataacaaca
     1141 aaaattgcta ctacaattgc ataggttata ataagcgcaa ggggttttct gaagttagca
     1201 aggaattttc gctttcgccc cattctgccg aaaactttgg agtagaaaaa tttgtaagga
     1261 tagttaagga tgtaagcaaa aaggaacccg attgcaaacg gagtgattat gcctgttatc
     1321 caaccgattg ccgaccacac aacattcata ttatctttta caagtaaaag aaataccgta
     1381 aatgaaatgc aaatcagcag agtttttagc gaaagcagtt ttttcattta aacaactcct
     1441 taaaataacg acatctgtga gctttccggc aaattattga ggactcc
//
LOCUS       DAOMVE010000229         1442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_25712_length_1442_cov_2.456381, whole genome shotgun sequence.
ACCESSION   DAOMVE010000229 DAOMVE010000000
VERSION     DAOMVE010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25712_length_1442_cov_2.456381"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLS64_09055"
     CDS             <1..212
                     /locus_tag="PLS64_09055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRL44002.1"
                     /translation="ALILLGVIDAPVSILGDLSFSLIKRNYGIKDYGSIFPGHGGMLD
                     RFDSIIFTAPVLVAINQFIPFITVG"
     gene            219..1364
                     /locus_tag="PLS64_09060"
     CDS             219..1364
                     /locus_tag="PLS64_09060"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRL44003.1"
                     /translation="MVNSLSILGSTGSIGTQTLDVVDKLNLKVSALTASTNIKLLEEQ
                     VRKYKPELAVVFNEEKAKEFKDNIKDTNTRVLSGMDGLIEAATLENADLVVNSVVGMV
                     GLKPTLAAANAKKDIAFANKETLVTGGKLVCDAVKKNGVKLLPVDSEHSAIFQCLQGM
                     PEKKMLKKLILTASGGPFFGKTTEELKNVTVKEALNHPNWSMGAKITIDSATMMNKGL
                     EIIEARWLFDVQPENIDVVVHRESIIHSLIEYVDNSVIAQLGLPDMRIPIQYAITYPE
                     RYESPVGELSLAQIGKMTFFEPDYDTFKCLRACKKALSLGGVATAIANGANEEANRLF
                     REGKITFLEIGDLVMGAIDNIDNFEPLCVDDVLKADKLAREYVLSKL"
BASE COUNT          449 a          266 c          306 g          421 t
ORIGIN      
        1 gggcattgat tcttctcggt gtaattgacg ctcctgtttc aattctcggt gatttaagct
       61 tttcacttat caaaagaaat tacggaatca aggactacgg ttcaattttt cccggtcacg
      121 gcggaatgct tgacaggttt gacagcataa tatttactgc ccctgttctt gtggcaatca
      181 atcaattcat acctttcata acagtggggt aaactaaaat ggtaaattca ctttcaattc
      241 tcggatcgac gggttcaatc ggaactcaga cgcttgatgt tgtagataaa cttaatttaa
      301 aagtttctgc gctcacggcg tcaacaaata taaagctgct tgaagaacag gtaagaaaat
      361 acaaacctga acttgcagta gtttttaacg aagaaaaagc caaagaattc aaagataata
      421 taaaagatac aaacacccgt gttttaagcg gtatggacgg acttattgag gcggcaacgc
      481 ttgaaaatgc cgaccttgtt gtaaattcgg ttgtcggaat ggtaggctta aagcccacac
      541 ttgcagcggc aaacgctaaa aaagatattg cttttgcaaa caaggaaacg cttgtaacag
      601 gcggtaagct tgtatgtgat gcggtaaaga agaacggtgt gaagcttctt cccgttgaca
      661 gtgaacattc cgctatcttc caatgcctgc agggaatgcc tgaaaagaaa atgcttaaaa
      721 agctcattct tacagcttca ggcggtcctt tcttcggaaa aacaaccgaa gagttaaaga
      781 atgtaacagt taaagaagct cttaatcatc caaattggag tatgggtgca aaaataacca
      841 tcgactctgc tacaatgatg aacaaaggtc ttgaaatcat tgaggcgaga tggctttttg
      901 atgttcagcc cgaaaacatt gatgttgttg ttcatcgtga aagtatcata cattctctca
      961 tagaatatgt agataattcc gtaattgcac agcttggact ccccgatatg aggattccga
     1021 tacagtacgc aattacatat ccggaaagat atgagtcacc tgtcggtgaa ttaagtcttg
     1081 ctcaaatcgg caaaatgact ttctttgaac ctgactatga tactttcaaa tgtctgagag
     1141 cctgtaaaaa agctctttca ctcggaggcg ttgcaacagc aattgccaac ggtgctaatg
     1201 aggaggctaa ccgtcttttc agagaaggta aaatcacttt tcttgaaatc ggtgaccttg
     1261 ttatgggtgc aattgataac atagacaatt ttgagccttt atgcgttgat gatgtgttaa
     1321 aggcagacaa acttgcaaga gagtatgtac tctctaagct ataatttgcg gtaatatttt
     1381 tcactgctgt ctttgcgacg gcaatgaata ttttggagtt gatttatttt aatgcaaatt
     1441 tt
//
LOCUS       DAOMVE010000230         1427 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_26032_length_1427_cov_3.512391, whole genome shotgun sequence.
ACCESSION   DAOMVE010000230 DAOMVE010000000
VERSION     DAOMVE010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26032_length_1427_cov_3.512391"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1290)
                     /locus_tag="PLS64_09065"
     CDS             complement(178..1290)
                     /locus_tag="PLS64_09065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRL44004.1"
                     /translation="MQFKLLSAIGIIIIVSGHCYHGGMELAYNWFPPYSYNLALFVFI
                     SGYFYKTDYEENVGKYIWKRTKRLLIPAYLWNIFYGGMVAFLGLFGFTIGAKPDLYNL
                     FVMPFVDGEAFQYNLGSWFVYPLFLVCIINVLFRKFLKLIHLDNEFIVLIVYLAIGMI
                     GINTAIGNPTAINGIVKLLVRTMFFLPCYEFGRFYKAVLEKKDTLNNVAYFAIIFAVQ
                     LILLTFCKDLEYTPSSFTKFNNGFIIPYISSITAIAFWLRVSRLLVPAIGNSKLVRLI
                     ADNTYGIMVNQLVGFMCLKFVFYGLSRITSGSLFGDFNVASFKSSIWYYYLPNGLQQW
                     AFVYLIFGLFVPILISIILNKICHIAQKHIVKFCNK"
BASE COUNT          511 a          263 c          199 g          454 t
ORIGIN      
        1 aattgatttt atatttttaa atcaattcgc tacttctgta atttaaaatt aagcagtttc
       61 caatatttta tatttgcaat atagctttgg tattgtaatt acatttatta atcaatttat
      121 ctatcttgta agacaatttt aacatatcaa atatataaac gcaatgattt ttagatatta
      181 tttgttacaa aatttcacta tatgtttttg tgcaatatga cagattttat ttaaaataat
      241 gctaattaat atcggtacaa acagaccaaa tataagatat acaaaagccc actgttgcaa
      301 accgtttgga agatagtagt accatatact tgatttaaat gatgcaacat taaagtcacc
      361 aaacaatgag cctgaagtaa tgcggcttaa tccgtagaat acaaatttta ggcacataaa
      421 acctacaagc tgatttacca taattccgta tgtgttgtca gctataagcc ttacaagctt
      481 gctgtttcct atggcaggta caagcaatct tgaaactctc agccaaaatg caattgctgt
      541 gattgacgag atatacggaa ttataaaacc gttgttgaat ttagtaaatg atgacggcgt
      601 gtactccaaa tctttgcaaa aagttaaaag tatcagctgt acagcaaata taattgcaaa
      661 gtacgcaaca ttattgagag tgtctttctt ttcaagcaca gctttataaa agcgaccgaa
      721 ttcataacac ggcaggaaaa acattgttct gacaagaagt ttcactatac cgttaattgc
      781 agtcggattt cctattgcag tgttaattcc tatcattccg attgcgagat acactatcaa
      841 aactataaat tcattgtcaa gatgaatcag tttcaaaaat tttctgaaca gcacatttat
      901 tatacaaacc aaaaacagcg ggtacacaaa ccatgaaccg aggttatatt gaaaagcctc
      961 accgtcaaca aacggcataa caaaaagatt gtataaatca ggctttgcac ctatagtaaa
     1021 tccaaacaaa ccgagaaatg caaccatacc accgtaaaat atattccaca gatacgcagg
     1081 aataagaagc cgctttgttc gtttccatat gtattttccg acattttctt cataatcggt
     1141 tttgtaaaaa tatcccgata taaatacaaa cagtgccaaa ttatacgaat acggcggaaa
     1201 ccagttataa gcaagttcca ttccgccatg ataacaatgt ccggaaacta taatgataat
     1261 tccgattgcc gacaaaagtt tgaattgcaa attcacccct ccgtcagtat taacgctacg
     1321 caaatttcta ccctttttca aaattcaact ctccttaatt tttgatttat attaaatatt
     1381 actgcttatt ttccctttta ctttgttaaa tcatatctca aaacagc
//
LOCUS       DAOMVE010000231         1410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_26474_length_1410_cov_2.433210, whole genome shotgun sequence.
ACCESSION   DAOMVE010000231 DAOMVE010000000
VERSION     DAOMVE010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26474_length_1410_cov_2.433210"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..>1410)
                     /locus_tag="PLS64_09070"
     CDS             complement(176..>1410)
                     /locus_tag="PLS64_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HRL44005.1"
                     /translation="LITCAVAFFGCSCSLTDDSESSEPKRSSIIEKSVTTPSAQDVEN
                     DKDYADVKTDYAYETLTDDYVKELYMRIAKSVDTVYAPNITTTGSLSDYQITQAVEAY
                     KNDHPETFWLKGSSLFVPYGDTTSVKLDYTVQNDELIAAKKKFDTAVTSALKKLPSGN
                     DFEREEYINNYIIDNCRYDEEAAENNDVQGNENDAYGALVDGKAVCEGYARAFQLLCN
                     KANIDCVLLSGTADSDNHAWNGVKIGGDWYQIDVTWNDTDGENNYAENDYFNLTDDLM
                     FKDHKLSYKYSELNSQTYLSVATWCNFYVPKCTAEKYNYFNYKYPTVSDPDNADDVSQ
                     AIANAAKNGDEYFSIVVDKNTDFDYMYDRIMNDGYLYNWVSEANEINGYSPRLAESCY
                     VYRKDELGVLTVELEYVD"
BASE COUNT          421 a          303 c          236 g          450 t
ORIGIN      
        1 cagaagaatt atcagaacaa ttaaaaagaa caacaaaaaa attattatta aaagatcctc
       61 aacgagaatt attatcaaaa gtttttttta tattacctta tatttatata aaatcaattt
      121 ttaagcagag tgtccctgca ttgcggacaa aagtgagcat ctcatcacat cgccgttaat
      181 caacatattc aagttcaaca gtcagcacac ctaactcatc cttgcgataa acataacagc
      241 tttccgcaag ccttggtgaa taaccgttaa tctcattagc ctctgaaacc cagttataaa
      301 gataaccgtc attcataatc ctgtcataca tataatcgaa atcggtattc ttatcaacaa
      361 caatgctgaa atattcatct ccgttttttg cagcatttgc aatagcttgg cttacatcat
      421 cggcattatc aggatctgaa acagtcgggt acttatagtt aaaatagttg tacttttcag
      481 ccgtacactt tggaacataa aaattacacc atgtagcaac gctcaaataa gtttgtgaat
      541 taagctcgga atacttatat gacagcttat gatccttgaa cataaggtca tcggttaggt
      601 taaaataatc gttttccgca taattatttt cgccgtcggt atcattccat gtaacatcaa
      661 tttggtacca atcgccgccg atttttacac cgttccatgc gtgattgtct gaatcggctg
      721 ttcccgaaag taatacgcag tcaatattcg ccttgttgca caaaagctga aaggctcttg
      781 cgtaaccctc acacacggct ttgccgtcaa ccaacgcacc gtaggcatcg ttttcattgc
      841 cctgaacatc attgttttca gccgcttctt catcatacct gcaattgtca attatatagt
      901 tgttaatgta ttcttctctt tcaaaatcat tgccggaggg cagttttttc aaagcagaag
      961 taacggcagt gtcaaatttt ttcttggcgg ctataagctc atcattttga acagtataat
     1021 caagctttac gcttgttgtg tcaccatacg gaacaaaaag acttgacccc ttgagccaaa
     1081 aagtttcagg gtggtcgttt ttgtatgcct caacagcttg tgtaatctga taatcggaaa
     1141 gcgagcctgt tgttgtaatg tttggtgcat acactgtgtc aacgctcttt gcaattctca
     1201 tatacagttc ttttacataa tcatcggtaa gcgtctcgta ggcataatcg gtttttacat
     1261 ctgcataatc cttgtcgttt tcaacatctt gtgcactcgg agttgtaacg gatttttcta
     1321 tgatagagct tctctttggt tcactacttt ccgaatcatc cgtcaacgaa catgaacagc
     1381 cgaaaaatgc aaccgcacag gttattaaac
//
LOCUS       DAOMVE010000232         1362 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_27706_length_1362_cov_2.094109, whole genome shotgun sequence.
ACCESSION   DAOMVE010000232 DAOMVE010000000
VERSION     DAOMVE010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27706_length_1362_cov_2.094109"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1362
                     /locus_tag="PLS64_09075"
     CDS             <1..>1362
                     /locus_tag="PLS64_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HRL44006.1"
                     /translation="NDAFGSCEKLTSVNFSETLKSIGAFAFVECKKLSEITIPQSVTS
                     VGEYAFSGCENLASVTVSDDLPYVGGRAFEKTKWLNSQPDGVVYIGKSAYGYKGDMPK
                     NTELSLKSGITNISGYAFYEEKNLTSVKIPETVSRIGNWAFLDCEGLKNVNIPDGVKR
                     IESWTFSNCSSLTNITVPDSVTVLDGLAFSYCTNLKNIELSKNLTEIGMGALSHCTSL
                     ETIDIPDSVIIMDNIAMAGCSELKSVNIGSNLKTVGGQVFAGCTSLEKVNVNLNNKNY
                     TSENGIWYDKNKTKIILYPYNKKDSAYTTPTSLKELCNGYVGSYGMLLDNSNLKTVTI
                     EKNVAKIDDYAIGFVFDFDNYKINKVKDFTVKGYRGTVAESYAKKNSFNFVALDKTLQ
                     TPSISKLENTSGGIKISWNKVSGAYGYRVYQKTSNGWKRIKDTTATSYTDSAVSVNQT
                     KTYT"
BASE COUNT          465 a          219 c          279 g          399 t
ORIGIN      
        1 aatgatgctt ttggctcttg tgaaaaactt acaagcgtta atttctcgga aaccttgaaa
       61 agtatcggcg cttttgcttt tgttgagtgt aaaaaacttt cggaaataac aattcctcag
      121 agtgttacaa gtgtgggcga gtatgcgttt tcaggttgtg aaaatcttgc aagtgttaca
      181 gtttcggatg atttaccata tgtcggcgga agagcttttg aaaaaacaaa gtggcttaac
      241 agtcagcctg acggtgtggt atatataggc aagagtgctt acggttacaa aggcgatatg
      301 cctaaaaata cagaattatc cctcaagtcg ggcattacca atatctcggg ttatgctttt
      361 tatgaagaaa agaatctgac aagtgtgaaa attcccgaaa cagtaagcag aatcggtaat
      421 tgggcatttt tagattgtga gggactgaaa aatgtaaata ttcctgacgg tgtcaaacgc
      481 attgaatcat ggactttttc taattgttca agtttgacaa acattactgt tcccgacagc
      541 gttacggttc ttgacggact tgctttctct tattgcacca atctgaaaaa tatagagctt
      601 tcaaagaatt tgaccgaaat cggaatgggt gcattatcgc attgtacaag tcttgaaact
      661 attgacatcc ccgacagcgt gataattatg gataatatag caatggcagg atgttcggaa
      721 ttaaagagtg ttaatatagg aagcaatttg aaaacagtag gcggacaggt atttgcagga
      781 tgtacttcgc ttgaaaaggt gaatgtcaac ctcaataata aaaactatac ctctgaaaac
      841 ggtatttggt atgacaagaa taagactaaa attattcttt atccgtataa caaaaaggat
      901 tctgcctata caacacctac ttcattaaaa gaactatgca atggttatgt cggaagttac
      961 ggtatgctac ttgataactc aaatcttaaa acagttacaa ttgagaaaaa tgtagctaaa
     1021 atagacgatt atgcaatcgg ctttgttttt gattttgata attataaaat aaacaaggtt
     1081 aaagatttta ctgttaaggg ttacagaggt actgtcgcag aatcttacgc taagaaaaac
     1141 agctttaact ttgttgcact tgataaaact ttgcagacac cgtcaatttc aaaacttgaa
     1201 aatacttcgg gcggtatcaa aataagctgg aataaagttt cgggtgctta cggttacaga
     1261 gtttatcaga aaacttcaaa cggttggaaa cgaattaagg acacaactgc aacaagttat
     1321 accgattcag cggtaagcgt aaatcagaca aaaacctaca ca
//
LOCUS       DAOMVE010000233         1319 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_28944_length_1319_cov_4.330696, whole genome shotgun sequence.
ACCESSION   DAOMVE010000233 DAOMVE010000000
VERSION     DAOMVE010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28944_length_1319_cov_4.330696"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..1247
                     /locus_tag="PLS64_09080"
     CDS             321..1247
                     /locus_tag="PLS64_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I repeat-containing protein"
                     /protein_id="HRL44007.1"
                     /translation="MKKLLSIILIGILAVSSFILTTFAAENEKSETEIEREKLGWLLD
                     AFKILNTNIGEARIYNCVAGDTYNAAYNLYNQDELATAEEYKNMKDKLYSAAYNLHVD
                     VAYAKTAYDNACKEQNYNNWYSDEQWNEFQYKLADLKTDLEPYIAGTNDYDVWAITNA
                     YNELLAVYNKMTNAYTLKGDVNKDGVVNVVDATLIQKYIAHEVEFTGAQKMLTNVAKY
                     ESDISVIAATDLQKFIVGLNGDFGNNHIFLDEQDGIQYFDDTMRWYAYGNYNISPRYP
                     YITYGPYVKNEHTDFFVVGYYDMMCKEHGYTK"
BASE COUNT          436 a          213 c          246 g          424 t
ORIGIN      
        1 actttaaccc gattggtcat tccaatatga atttaactaa tctttcttct taatcttctt
       61 tcctttattc agttttcagg gtgtttaccc ttttatatac accgagtaat cggtggttca
      121 tttgaacttt cagcaccaca taatgtggtg cttttttgtt tatatttcac aaaaaatgaa
      181 ggaatatttt ataaccaaat attgaaatag tattgattat aattactgta tatggtacac
      241 tgataacagt tctaaaaact atgatgtaaa tttggatggt acagcacatt gatacagaag
      301 atgatatagg gggataaaca atgaaaaaat tattatcaat catactcatt ggaattttgg
      361 cggtttcatc tttcatttta acaacatttg ctgctgaaaa tgaaaagtcc gaaactgaaa
      421 tagagcgtga gaaacttggc tggctccttg acgcatttaa aattctcaac acaaacattg
      481 gtgaggcgag aatctataac tgtgttgcag gtgatacata taatgctgca tacaatcttt
      541 ataatcagga cgaacttgca acagccgagg aatataaaaa tatgaaggac aagctgtata
      601 gtgccgctta taaccttcat gttgatgttg catatgctaa aactgcttac gataatgcct
      661 gtaaggaaca gaattacaac aactggtatt ctgatgagca atggaacgaa tttcagtata
      721 agcttgctga tttaaaaacc gacttagagc cgtatattgc aggcactaat gattacgatg
      781 tttgggctat tacaaatgcc tacaacgaac ttcttgctgt gtacaacaag atgacgaatg
      841 cctatacttt aaaaggtgat gtgaacaaag acggtgttgt taatgttgtc gatgctaccc
      901 ttattcagaa atatatcgcc catgaggttg aatttacagg tgcgcagaag atgttaacaa
      961 atgtagcaaa gtatgaaagc gatatatcgg tcattgccgc tactgattta caaaaattta
     1021 ttgttggtct taatggcgac tttggaaaca atcatatttt ccttgacgag caggatggta
     1081 tacagtattt tgatgataca atgcgttggt atgcttacgg taactataat atttctccaa
     1141 ggtacccgta cattacctat ggtccgtatg ttaaaaatga gcatacagac tttttcgtag
     1201 tcggttatta cgatatgatg tgtaaagaac acggctatac aaaataattt cttcagatta
     1261 aaattgaaaa tataaaatcg gcggaagtaa tgcacaaaca cattgcttcc gctgttttt
//
LOCUS       DAOMVE010000234         1316 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_29037_length_1316_cov_1.659794, whole genome shotgun sequence.
ACCESSION   DAOMVE010000234 DAOMVE010000000
VERSION     DAOMVE010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29037_length_1316_cov_1.659794"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLS64_09085"
     CDS             complement(<1..544)
                     /locus_tag="PLS64_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL44008.1"
                     /translation="MKLKERMQNSFFALSDNRLLGRKKLAISYVSLFLVFTLVLVTTV
                     AWFTVKDTASINSQAFSLESSAALRVNDGQEDLSNHLVVKGFKLEEASSVDGRNMFFP
                     SEGNFKDSTSAMKFREGTAGDRNKTYVYKDFKLNADSDMTNVYIKGYNITIVSADGKT
                     VLGKFDGSTEIIRNDKGVPVD"
     gene            complement(573..>1316)
                     /locus_tag="PLS64_09090"
     CDS             complement(573..>1316)
                     /locus_tag="PLS64_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL44009.1"
                     /translation="EVSSDGYTFYKPPLKQENGVASPILDVADWDLADYSVHYLSFDL
                     YVRSKSKFDIYLDSKSKISPNAKNLTGENADNKSDFGESGDSKGISRDCVTGAVRFSV
                     ANYDTNETKLLWIPAPNIFLNVNSDTTQYSVNTDKSSGESYSHVYYEKDGSRWVKKSV
                     SVNDGTSLGQVFVANSNGFTTNGDYTYELGSKKKIMTLEKDTTKNDKYTGMVTCNMWV
                     DGEDAEARLALVNGKFKANLVLSKGDVQQ"
BASE COUNT          365 a          288 c          241 g          422 t
ORIGIN      
        1 gatcgacagg aacgccttta tcgttacgaa taatctcggt tgagccgtca aacttaccga
       61 gaacagtttt accgtctgcc gaaacaattg taatattata gcctttgata taaacatttg
      121 tcatatctga atcggcatta agcttaaaat ccttatatac atatgtttta ttcctgtcgc
      181 ctgcagttcc ttcacggaat ttcatggcag atgttgaatc cttaaagttg ccctctgacg
      241 ggaagaacat attccttccg tcaacgcttg aagcctcttc aagcttaaag cctttaacaa
      301 caaggtggtt tgaaagatct tcctgaccgt cattgacacg gagtgccgca cttgactcaa
      361 gtgaaaacgc ctgagaatta atacttgccg tatcttttac ggtaaaccat gccaccgttg
      421 tcacaagcac aagagtaaaa acaaggaaca atgatacata tgaaattgcg agcttttttc
      481 tgccgagaag tctgttatcg gaaagtgcaa aaaacgaatt ctgcattctt tctttcagtt
      541 tcatacttca ttgctccttt cctgattttt atttattgtt gtacatcgcc ctttgagaga
      601 acaaggtttg ctttaaactt tccgtttaca agggcaagtc ttgcctctgc gtcttcgccg
      661 tcaacccaca tattacaggt taccattcct gtatatttgt catttttagt tgtgtccttt
      721 tccaaagtca taattttttt ctttgaacca agctcgtatg tgtaatcccc gttggttgta
      781 aagccgttgc tgtttgcaac aaaaacctga ccgagtgatg taccgtcatt aacggaaacg
      841 gattttttaa cccatctact accgtctttt tcataataaa cgtgcgaata tgattcgccc
      901 gaactcttgt ctgtgtttac agaatactgt gttgtatctg agtttacatt aagaaaaatg
      961 ttcggagcag gaatccacaa aagctttgtt tcgttagtat cataatttgc aaccgaaaac
     1021 cttaccgcac cggttacgca gtctcttgaa attccctttg agtcaccgct ttcaccgaaa
     1081 tcactcttat tatctgcatt ttcacctgtc agattttttg cattcggact gattttagat
     1141 ttactgtcca gataaatatc gaacttggat tttgaacgaa catacaggtc aaaagaaagg
     1201 tagtgaaccg aatagtcggc aagatcccag tcggcaacat caagaatagg agaagcaact
     1261 ccgttttcct gtttgagggg cggcttataa aatgtgtatc cgtcgctcga aacctc
//
LOCUS       DAOMVE010000235         1277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_30210_length_1277_cov_2.745499, whole genome shotgun sequence.
ACCESSION   DAOMVE010000235 DAOMVE010000000
VERSION     DAOMVE010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30210_length_1277_cov_2.745499"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLS64_09095"
     CDS             <1..375
                     /locus_tag="PLS64_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRL44010.1"
                     /translation="ESGKKFFAEQSCCAVAVDENGEILGMYILHPNNIGRCGHICNAS
                     YAVSSSARGKGVGRKLVSDCLERAKKYGFKILQFNAVVKTNTSARNLYESLGFVKLGV
                     IPKGFLMKDGHYEDICPYYKEL"
     gene            406..738
                     /locus_tag="PLS64_09100"
     CDS             406..738
                     /locus_tag="PLS64_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRL44011.1"
                     /translation="MIIDFKNMEEQILPNFKGGEKAYAVKKFTDDRNMIMHGRLESGA
                     SIGLHTHEGNCEMFYILEGTGKVLMDGEYERVEAGVCHYCPKGHTHSLINDSDSDLVF
                     FAVVAEQK"
     gene            821..1042
                     /locus_tag="PLS64_09105"
     CDS             821..1042
                     /locus_tag="PLS64_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL44012.1"
                     /translation="MNRTAEILGEKWDLPLGFTMALGENPDAMFVFSSMPKKYRESCI
                     DRARNANSRSEMQRIVSDIAQKSFNDFIG"
     gene            complement(1091..>1277)
                     /locus_tag="PLS64_09110"
     CDS             complement(1091..>1277)
                     /locus_tag="PLS64_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL44013.1"
                     /translation="CEILDASFFIKDGLIYWCDNGDVKEDDIHNYKGTVICSKKVRWR
                     NASELIGKRDFYKSANE"
BASE COUNT          395 a          237 c          276 g          369 t
ORIGIN      
        1 gaaagcggaa aaaagttttt tgcagaacaa agctgttgcg cagttgcagt cgatgaaaac
       61 ggcgagattc tcggaatgta tattcttcat ccaaataata tcggcagatg cggtcatatt
      121 tgtaatgcaa gctatgcggt ttcttcttct gcaaggggaa aaggtgtcgg cagaaagctt
      181 gtgtcggatt gccttgagcg tgctaaaaaa tacggcttca aaattttgca gtttaacgct
      241 gttgtaaaaa ccaacacatc tgcaagaaat ctttatgaaa gcctcggctt tgtaaaactc
      301 ggagttattc cgaaaggctt tttgatgaaa gacggtcact atgaggacat ctgtccttat
      361 tataaagaac tttaaaattt caatttaatc aggaggacac gagatatgat tattgatttc
      421 aagaatatgg aagaacaaat tttgccgaat tttaaaggcg gcgaaaaggc atatgccgta
      481 aagaaattca cggatgacag aaatatgatt atgcacggtc gccttgaaag cggtgcgtca
      541 atcggacttc acactcacga gggcaactgc gaaatgttct acatccttga gggaacaggc
      601 aaggtgctta tggacggcga gtatgaaagg gttgaggcag gcgtgtgcca ctattgcccg
      661 aaaggtcata cacacagcct tattaatgac agcgactccg accttgtatt ctttgctgtt
      721 gttgcagagc agaagtaatt ttattttcgg tttatgattt ccgattattc tctgttgtat
      781 cttaaatccc tatgcaaaaa ctaaaacagg ggtgattacg gtgaacagaa ctgcagaaat
      841 actcggagaa aaatgggatt tgccgcttgg ctttacaatg gcactcggtg aaaatcccga
      901 cgcaatgttt gtcttttcaa gtatgccgaa aaagtaccgt gaaagctgca ttgacagagc
      961 gagaaacgca aattcccgtt ccgaaatgca gagaatagta tcggatatag cacaaaaatc
     1021 ttttaatgat tttataggtt aacaaaaaag caacggagca aaaacttcgt tgcttttaaa
     1081 ttgctgtctg ttattcgttt gcacttttat aaaaatctct tttaccgata agctcgcttg
     1141 catttctcca acgaactttc tttgagcaga tgaccgtgcc tttataattg tgtatgtcgt
     1201 cttcctttac atcaccgtta tcacaccaat aaataagacc gtcttttatg aaaaatgatg
     1261 cgtccaatat ttcacag
//
LOCUS       DAOMVE010000236         1242 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_31299_length_1242_cov_4.498736, whole genome shotgun sequence.
ACCESSION   DAOMVE010000236 DAOMVE010000000
VERSION     DAOMVE010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31299_length_1242_cov_4.498736"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..>1242)
                     /locus_tag="PLS64_09115"
     CDS             complement(38..>1242)
                     /locus_tag="PLS64_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRL44014.1"
                     /translation="TGSPITDIEITLVSGKAHAKHTEGGDFRQATYRAVRQGLRSAKS
                     ILLEPVYDFTLEVPNENVGRAMSDIQRMSGTFNSPEAKGENSVLTGTAPVSEMGNYTK
                     EVMQYTHGRGRLSCSLKGYEPCHNSDEVIAQIGYDCDADVDNPCGSVFCSHGAGYNVP
                     WNEVGEHMHLPSVLDAPKDDEIGTNSENAFAKCKTQDDIFALDKELMQIFEQTYGPVT
                     RRKHAPEKRHVKAVSSIDKAAEKYKKSPVYDGTEYLLVDGYNVIFAWEHLKELSERSL
                     DGARHALINILCNYQGYSKCNLILVFDAYKVKGEHREVETVNNISIVYTKEAETADMF
                     IEKTSHKLAKTNKVRVVTSDALEQMIILGNGALRVSSRAFLEEVRQAENEIREIINSS
                     NELNNNMN"
BASE COUNT          291 a          301 c          269 g          381 t
ORIGIN      
        1 atcgctgtat ctgtcggctt attattatgc ttaaaaacta attcatatta ttgtttaatt
       61 cgtttgaaga atttattatt tctctgattt cattttcggc ttgtctgact tcttctaaaa
      121 atgctcttga tgaaacacgc aacgcaccgt tgcccagaat tatcatctgt tcgagtgcgt
      181 cggatgttac aacacgcacc ttgttggttt tggcaagctt gtgagaggtt ttctcaataa
      241 acatatcggc ggtttctgcc tctttggtat atacaatgct gatattattc acggtttcaa
      301 cctctctgtg ttcgcccttt accttgtacg catcaaacac gagaataagg ttgcacttgc
      361 tgtagccctg atagttgcag agaatgttta tcagagcgtg ccttgcaccg tcaagacttc
      421 tttcggaaag ctctttgagg tgttcccacg caaaaataac attgtagccg tcaacaagca
      481 gatactccgt gccgtcatac acgggggatt ttttatactt ttcagccgcc ttgtcaatag
      541 acgaaaccgc cttgacatga cgcttttcgg gagcgtgttt tctgcgtgtc acaggaccgt
      601 aggtctgttc aaaaatctgc ataagctcct tgtcgagagc aaaaatatcg tcctgagttt
      661 tgcactttgc aaaagcgttt tcgctgtttg tgccgatttc atcgtctttc ggagcatcga
      721 gaacactcgg cagatgcata tgctcgccga cctcattcca cggcacattg tagcctgctc
      781 cgtgagaaca gaacacagaa ccgcatggat tgtcaacatc ggcatcgcaa tcgtagccga
      841 tttgtgcaat aacttcatcg ctgttgtgac acggctcata gcctttaaga ctgcacgaaa
      901 gtctgcctct gccgtgagtg tactgcataa cttcttttgt gtagttgccc atttcggaca
      961 cgggtgcagt acctgtgaga acggaatttt ctcccttggc ttcgggagaa ttgaaagtac
     1021 cgctcatccg ctgaatatct gacattgccc gtcccacatt ttcgttaggc acttcaagcg
     1081 taaaatcata aacaggctca agcagaatgc tctttgccga gcgcagtccc tgtcttacgg
     1141 cacggtaggt tgcctgtctg aaatctccgc cctcggtatg ctttgcgtga gcttttcccg
     1201 aaacaagcgt gatttcaata tctgtaatcg gcgagcctgt ca
//
LOCUS       DAOMVE010000237         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_32503_length_1207_cov_2.050347, whole genome shotgun sequence.
ACCESSION   DAOMVE010000237 DAOMVE010000000
VERSION     DAOMVE010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32503_length_1207_cov_2.050347"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..967)
                     /locus_tag="PLS64_09120"
     CDS             complement(185..967)
                     /locus_tag="PLS64_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRL44015.1"
                     /translation="MCKIISIANQKGGVGKTTTAFNIATALALKNNKVLAIDFDPQAD
                     LSKYAAFDNTDGNPTMSDLLIGKVGMSKAVEIKDCIRHNEVNHLDYIPCDINLANADL
                     FLAGVLSRETILKRILDNDIIKQYDYVIIDCNPSLGVLLMNALTASTGVIIPVQVQDF
                     AFGGLKTLTGVISQIQETINPNLRILGVLPTMVDRTNASKRVIEQLESIYNNALFNTQ
                     IHRATQAVESTKQHKALCLIKGSKLGEEYIACAEELTKRIGV"
     gene            complement(982..>1207)
                     /locus_tag="PLS64_09125"
     CDS             complement(982..>1207)
                     /locus_tag="PLS64_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL44016.1"
                     /translation="CVKKFDVTDVGEQHDLMYIFQKPEKSKLQSKSVETKENGTKGKA
                     PISRKQIKNNAKIISQKNTVSKEKQTDKSR"
BASE COUNT          303 a          238 c          239 g          427 t
ORIGIN      
        1 ataggtgtca ttactccgtt tttgcggatt gattcaacca tatcatccct gcgttcgccg
       61 tcgtaaagag aaaactgatg attgtgaaac ggtacaagca tatcaagcgg tatttgcttt
      121 atttgattgt tttgcgagag cattgctcca aagttaaagc ctgtcattgt cgttcctccg
      181 taaattacac accaattctt tttgtcagtt cttcggcaca agctatgtat tcctctccga
      241 gcttactgcc tttaattaag cataatgctt tgtgctgctt agtgctttca actgcctgtg
      301 tggcacggtg tatctgagtg ttaaaaagag cattattgta aatgctttca agttgctcaa
      361 tgacacgctt gcttgcgttt gtgcggtcaa ccattgttgg cagcacaccg agaattctta
      421 aattaggatt gatggtttcc tgtatttgtg aaataacacc tgtaagagtt tttaaaccgc
      481 cgaaggcaaa gtcctgcacc tgtaccggga taattacacc ggtgcttgcc gtgagagcgt
      541 tcatcagcaa aactccaagt gacggattac aatcaataat tacataatcg tactgtttga
      601 taatatcatt atcaagaata cgcttgagta ttgtttccct cgaaagtacc cctgcgagga
      661 ataagtctgc atttgcaagg ttgatgtcac aaggaatgta atcaaggtga ttaacctcgt
      721 tatgtctgat gcagtctttt atttcaactg ccttactcat acctacctta ccaataagaa
      781 ggtcgctcat tgttgggttg ccgtctgtat tgtcgaaagc ggcatatttg gataaatccg
      841 cctgtgggtc aaagtctatt gcaagcactt tgttgttttt gagggcgaga gcggttgcta
      901 tattaaaagc tgttgttgtc ttaccgactc cgcccttctg attagctatt gaaatgattt
      961 tgcacataat ttatacctcc gttatcttga tttatctgtt tgtttttctt tgctgactgt
     1021 atttttttga gagattattt tagcgttatt cttaatctgc ttacgagaaa taggagcttt
     1081 tcctttagtt ccgttttctt tagtttcaac cgatttagac tgcaactttg atttttcagg
     1141 cttttgaaat atgtacatca aatcgtgttg ttctccaaca tcggtaacat caaacttttt
     1201 gacacat
//
LOCUS       DAOMVE010000238         1070 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_37929_length_1070_cov_2.258128, whole genome shotgun sequence.
ACCESSION   DAOMVE010000238 DAOMVE010000000
VERSION     DAOMVE010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37929_length_1070_cov_2.258128"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..>1070)
                     /locus_tag="PLS64_09130"
     CDS             complement(126..>1070)
                     /locus_tag="PLS64_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL44017.1"
                     /translation="RIYDILLDKERDDMKRAIYISILCTVMLTACGNVSESTKRAETS
                     DVSQTAVTVTSAKEEMDTSKTTTTIKKSSQTVSSSSKPTDTEVTAAKKSSTSNTGGNS
                     VQGQNNAVQGNNQAVQNNTPSNNSQDNNLAEQNAQPSNEKSQNNEPSATRSTTAKPSA
                     SAEPAVTNPPDTTTKPQTTKVPQTQPPLPVPTEEERYMDIGRSLSHNGSDYSKAEAVY
                     NWMTENGSGTCVNYSWQTYYLCKGTGLECYLAWTPSKLYGHVANVVKVNGTWFVLDTQ
                     GQGFLLNNDCGFSEIIDIDESYVSGADLISSQRYDEIY"
BASE COUNT          233 a          255 c          218 g          364 t
ORIGIN      
        1 agtcctgcca tgaaccctgc gctgagtttc tttaatattt tcgatttttt gaacataaag
       61 tgcatttctc cttttcattt ttgcaaagaa aaagagcctg atttcggctc tttcgtttgc
      121 acattttaat atatttcatc atatctttgg ctgcttatca aatccgcccc tgacacatat
      181 gattcatcaa tatctattat ttcgctgaat ccgcagtcgt tattcagcag aaatccttgt
      241 ccctgcgtat caagaacaaa ccatgttccg tttaccttaa cgacatttgc tacatgacca
      301 tacagttttg acggcgtcca tgcaagatag cattccaaac ctgtaccttt gcacaaatag
      361 taggtctgcc acgaataatt cacacaagtg cccgaaccgt tttcagtcat ccagttgtat
      421 accgcctctg ccttgctgta atcagagccg ttatgagata agcttctgcc gatatccata
      481 tatcgttctt cctcagtagg caccggcaag ggtggttgag tctgcggaac ttttgtagtc
      541 tgcggctttg tcgttgtgtc aggcggattt gttaccgcag gctctgccga tgctgacggc
      601 tttgccgttg ttgatcgggt ggcagacggc tcgttattct gagacttctc attggacggc
      661 tgagcgttct gctccgctaa gttattgtcc tgagaattgt tggatggagt attattctgc
      721 acagcttggt tattaccctg tacagcgtta ttctgcccct gaaccgaatt gccgcctgta
      781 tttgaagtgc ttgatttctt agccgcagtt acctcagtat ctgtcggctt tgaggaagat
      841 gataccgtct gcgaactctt tttaatggtc gttgtggttt tacttgtatc catttcttcc
      901 tttgccgatg ttaccgtgac cgcagtttgg ctcacatctg aggtttctgc tcttttcgtt
      961 gactccgata cattcccgca ggcagtcagc attactgtgc aaagtattga aatatatatc
     1021 gctcttttca tatcatcacg ctccttgtca agcaatatat catatatccg
//
LOCUS       DAOMVE010000239         1059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_38500_length_1059_cov_1.800797, whole genome shotgun sequence.
ACCESSION   DAOMVE010000239 DAOMVE010000000
VERSION     DAOMVE010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38500_length_1059_cov_1.800797"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /gene="cas9"
                     /locus_tag="PLS64_09135"
     CDS             complement(<1..1053)
                     /gene="cas9"
                     /locus_tag="PLS64_09135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01865.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HRL44018.1"
                     /translation="MVENLYISPAVKRPVYQSLKIMHEINKIQGHAPKKIFVEMTRKD
                     GVKGDKGRKESRKTKLVDLYKKCGEDSGELWESLEKTPDDEFKRDRLYFYYTQFGKCM
                     YTGEPINLSELYNQNIYDVDHIFPRSKVKDDSLDNRVLVKKQVNAHKDNTYPLDSSIR
                     EKMKGFWHLLMDKGLISKKKYERLTRATELTDSELSDFIARQIVETSQSTKAVASLFK
                     ELYPDTEIVYVKASTVSEFRHEYDFLKCREVNDFHHAKDAYLNIVVGNVYNERCEGLK
                     TKAYSLNKMFSFNTPNAWSIDDNKSIKIVRKTMNKNNIRFTRYAFTKNGELFKRKPLK
                     KGRGQAPLKQNSPLSDI"
BASE COUNT          278 a          214 c          183 g          384 t
ORIGIN      
        1 gatatccgaa agcggagagt tctgctttaa tggagcctgt cctcttcctt ttttaagggg
       61 ttttctcttg aaaagttcgc cgtttttggt gaatgcatac cttgtaaaac ggatattgtt
      121 cttgttcata gtctttctga caattttgat tgacttatta tcatcaattg accatgcgtt
      181 cggtgtatta aaactgaaca ttttgttcag gctgtaagct ttggttttca aaccttcaca
      241 gcgttcgtta tacacattgc cgacaacaat atttagatag gcatcttttg catggtgaaa
      301 atcgtttact tctctgcatt ttaagaaatc atattcgtgt ctgaattcag aaaccgttga
      361 cgcttttaca tacacaattt cagtatcggg atacaattct ttaaaaagtg acgcaactgc
      421 ttttgttgat tgagatgttt caacaatctg tcttgcaata aaatctgaaa gttcgctgtc
      481 agtaagctca gttgcccttg taagcctctc gtactttttc ttgctaataa gacctttatc
      541 cataagcaaa tgccagaagc ctttcatctt ttcacgaatg gagctgtcaa gcggatatgt
      601 attatccttg tgagcattaa cctgtttctt tacaaggaca cggttatcaa ggctgtcatc
      661 tttaactttt gaacgaggga aaatgtgatc cacatcataa atgttttgat tgtacaattc
      721 cgacagattt atcggttctc ccgtgtacat acacttgccg aattgagtat agtaaaaata
      781 cagtctgtct cttttaaatt catcatcagg tgttttttca agagattccc agagttcgcc
      841 cgaatcctca ccgcattttt tatataaatc tacaagcttg gtctttcttg actctttacg
      901 gcctttgtcg cccttcacac cgtcttttct tgtcatctca acaaatatct ttttaggtgc
      961 atgaccttga attttgttga tttcatgcat aattttcaaa gactgataca caggtcgttt
     1021 taccgcagga gaaatataga gattctccac cattttctc
//
LOCUS       DAOMVE010000240         1056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Ruminococcus bromii isolate AMR_MDS_5712
            NODE_38627_length_1056_cov_2.816184, whole genome shotgun sequence.
ACCESSION   DAOMVE010000240 DAOMVE010000000
VERSION     DAOMVE010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867933
            Sequence Read Archive: SRR1616981
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ruminococcus bromii (wastewater metagenome)
  ORGANISM  Ruminococcus bromii
            Bacteria; Bacillati; Bacillota; Clostridia; Eubacteriales;
            Oscillospiraceae; Ruminococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.113
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 21:33:02
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,829
            CDSs (total)                      :: 1,794
            Genes (coding)                    :: 1,775
            CDSs (with protein)               :: 1,775
            Genes (RNA)                       :: 35
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 11 of 19
            Pseudo Genes (internal stop)      :: 2 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Ruminococcus bromii"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38627_length_1056_cov_2.816184"
                     /isolate="AMR_MDS_5712"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:40518"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /collection_date="2012-06-14"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLS64_09140"
     CDS             <1..245
                     /locus_tag="PLS64_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRL44019.1"
                     /translation="IVGSGATALAMAFPNRFILYPLIFILWYIPSIAKQSIMMAFQPF
                     TEYPLSYALPSVIFVLATNVVAVLFSFFKVTKYDQV"
     gene            232..990
                     /locus_tag="PLS64_09145"
     CDS             232..990
                     /locus_tag="PLS64_09145"
                     /inference="COORDINATES: protein motif:HMM:NF035952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxPxxD family membrane protein"
                     /protein_id="HRL44020.1"
                     /translation="MIRCKLRKYAIVLPLCAVFFISLCTYTYLRFYNYLNSDYSEKLS
                     QKNIFYNVAGGDGDFITSGNVIGFYIILFVLFLFYIFTDENESYIVRLKSRASFVTGR
                     IADCAVFAFLFSFLIEAVSVVAALICFDTNLILESNFLQYSALELLTLFLFYFRAGLV
                     MLSFGIIISTKVAPIITIALIFTEFFADVAFMISRVWLPFRDAIVVSKLMNGEMVLSD
                     MWGIILRALLMMFMLIFSSYFLYQKKDVLSNVKK"
BASE COUNT          266 a          161 c          211 g          418 t
ORIGIN      
        1 gtattgtggg aagcggtgca acggcacttg caatggcatt tccaaacaga tttatccttt
       61 atccgttaat atttatattg tggtatatac cgtcaattgc taaacagtct attatgatgg
      121 cttttcagcc ttttaccgaa tatccattgt catatgcact tccttcagtt attttcgtat
      181 tagctacaaa tgtggttgct gttttgtttt cgttctttaa ggtgactaag tatgatcagg
      241 tgtaaattaa gaaaatatgc aatcgtactt ccgctttgcg cagtgttttt tatttcgctt
      301 tgcacataca catatttacg attttataat tatttgaatt cggattattc tgagaagtta
      361 agccaaaaaa atatttttta taatgttgcg ggcggtgatg gtgactttat cacatctggc
      421 aatgttatcg gtttttatat aatattgttc gttttgtttt tgttttacat atttaccgat
      481 gagaatgaaa gctacattgt aagactgaaa tcgagagcct cttttgtgac aggaagaatt
      541 gctgattgtg cggtatttgc ttttctgttt tcatttttga tagaagcagt cagcgtagtt
      601 gcggcactta tctgctttga cactaacctg attttagaat cgaattttct gcaatattcg
      661 gcacttgaac ttttgacttt gtttttgttt tatttcagag caggactcgt tatgctctcg
      721 ttcggaataa tcatttctac aaaagttgct ccaattatca ccatagctct gatttttaca
      781 gagttttttg cagatgtagc ctttatgata agtagggtat ggcttccgtt cagagatgcc
      841 atagtggtgt caaaacttat gaacggcgaa atggttctgt cggatatgtg gggcattata
      901 ttaagagcat tgcttatgat gtttatgctt atcttctctt cctacttttt gtatcagaaa
      961 aaggatgttt tgagtaatgt taaaaagtaa gcttgttttg tcgataatac tgattgttgc
     1021 gtgctttgct caattgttca gttgtgtatt tcaggg
//
